LOCUS       DAOPID010000001        31912 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_1345_length_31912_cov_4.646451, whole genome shotgun sequence.
ACCESSION   DAOPID010000001 DAOPID010000000
VERSION     DAOPID010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 31912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31912
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1345_length_31912_cov_4.646451"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="PK641_00005"
     CDS             complement(<1..339)
                     /locus_tag="PK641_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the hydrolysis of Xaa-His dipeptides;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HPG54771.1"
                     /translation="MTSTTLFWKYFDEICKVPRPSGNCSAIIKHIEQFAKTNNFDSSI
                     DSAGNIVIRKPATIGMESRPAIILQGHVDMVPQIAPGTIHDFTKDSIKYSEKDGKIFA
                     QGTTLGADNGV"
     gene            complement(342..2252)
                     /gene="dnaG"
                     /locus_tag="PK641_00010"
     CDS             complement(342..2252)
                     /gene="dnaG"
                     /locus_tag="PK641_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPG54772.1"
                     /translation="MIDQATKDRILTAAKIEEVVGDFVTLKKRGVNLIGLCPFHNEKT
                     PSFTVSPAKNICKCFGCSKGGSPVNFIMLHEQLDYADALRYLARKYNIEIIEKELSSE
                     EIEHRNDRESMFAINQFALTYFTKILWENEDGRALGISYFKERGFRDDIIKKFQLGFC
                     LDKYDSLSKEAIKSGYNKQFLLKTGLCTESERGGLVDRFRGRVMFPVHSLSGKIVAFG
                     GRILKKTDNAAKYVNSPESEIYHKSNELYGIFFAKNSIVKEDRCFLVEGYTDVISMHQ
                     AGIENVVASSGTSLTPGQIRLIHRFTSNITVLYDGDAAGIHASIRGIDMLLSEGMNVK
                     VVLLPDGEDPDSFARSRDSSEFNAFIKEHQTDFIRFKTQLLLDDAAGDPIKKANLIND
                     IVTSISVIPDTIIRSLYIKECADRFEMRESVLAEVLAKKISGIQKTIAQNEIKQAVVE
                     KEQEKPFAFMQEQNLIRYIIRYGKNLIPVEVDENKIEQISVLDYINQSLIADNMKLEY
                     GLHKHILQEAIVVEGDLRHYFINHPDESVRNYAFYLSEDKYERSNIHDKYNKTETEEE
                     RLRLIIPRITLEYKNKILEKQSNELIAALKQANAEHDHEKINELLEKQNELNKYRQTL
                     ARYLGERTIIAT"
     gene            complement(2342..3292)
                     /gene="fmt"
                     /locus_tag="PK641_00015"
     CDS             complement(2342..3292)
                     /gene="fmt"
                     /locus_tag="PK641_00015"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPG54773.1"
                     /translation="MEKKTLRIVYMGTPDFAVEPLKKLVEDSYNVVGVVTMPDKPAGR
                     GYQLQQSAVKKFALERNLPLLQPENLKDEGFLTSLREWNADLQIVVAFRMLPEVVWNM
                     PKYGTFNLHASLLPQYRGAAPINWAVINGDKETGVTTFFLKHEIDTGNIIMQSKVSIE
                     DDENVGDIHDKLMYLGASVVTETVDLIISGNYILTPQDESIVLKPAPKIFKDTCRIDW
                     SKSVIEIKNFVRGLSPYPAAFTEVKDEKDRVLSLKIYDVSVEETKISEPSGVLLTDNK
                     TYLKFTASDGYVQVHRLQLAGKKTMDVQDLLRGTHLKSVE"
     gene            complement(3362..3943)
                     /locus_tag="PK641_00020"
     CDS             complement(3362..3943)
                     /locus_tag="PK641_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPG54774.1"
                     /translation="MKNLVLYIINGVLVVAVVVLFILFFINKEENSKEQALTEGEVLP
                     IAYVNIDTLLLNYDLYKSMSEELLQEEERSRASVNQKANALQAEMVDFQNKIENRAFL
                     SEERARSEQERLVRKQRELQELDNKLAQDLVVKQKNMNDKLFKTLDSVVTVYNKDKKY
                     HFIFSNTGSNNILYGNKAYNITNEILELLNEKK"
     gene            complement(3940..4143)
                     /locus_tag="PK641_00025"
     CDS             complement(3940..4143)
                     /locus_tag="PK641_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005807575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG54775.1"
                     /translation="MLKAFLLSLVILIPAILLMGYRVFFTQKGKFPGLHVDSNAALRE
                     KGIHCVQTQDKEARKQKTNQIKA"
     gene            4379..5800
                     /locus_tag="PK641_00030"
     CDS             4379..5800
                     /locus_tag="PK641_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HPG54776.1"
                     /translation="MNQNMPNPIDVWKYFSEICKIPRPSKHEEKITAYLIRFAQKNNL
                     EYKQDEVGNVLIKKEGISPTVILQAHTDMVCEKNDNVIHDFMIDPINFTQDGDWISAN
                     GTTLGADDGIGVAMMLALLAGNETQRSLECLFTIDEETGLTGASALKNDFISANSLIN
                     LDSETDDEIIIGCAGGCDSILTFNIEKEPMPEHLFAISLKIFGLQGGHSGDDINKDRA
                     NANKLLNEFLNLASKQFDLSVCNINGGGLRNAIAREASATIAVPWADKERIRIAWNIF
                     SAETEDNWIKKEPGMKLSLDSCKVPDTRIKHDDALNIINAISECPHGVLEYSSEIPGL
                     VKTSTNLASIKTLENSIVICTSQRSDDIDKLHNTASMIDKTANKHHALVTHSNEYPGW
                     KPNFESPLVKKADSIYKQLFKKAPKIKVIHAGLECGLFLQKFPNLDMISIGPNLEGVH
                     SPDERVSVSSVEKTWRYLIELLK"
     gene            complement(5803..6576)
                     /locus_tag="PK641_00035"
     CDS             complement(5803..6576)
                     /locus_tag="PK641_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HPG54777.1"
                     /translation="MKILILGAGKMGTFLTDVLCVQHEVALFDLNPKNLRFVFNTYRM
                     TKYEEIKEFEPELMINCVTLKYTIDAFNAVIQYLPKTCIISDIASVKTGLPEYYKQCG
                     MRYVSTHPMFGPTFANLEDLSNQSAIIISESDHMGKAFFKDLYGSLKLKIFEYTFLEH
                     DETIAYSLSIPFASTMVFAAVMKHQEAPGTTFKKHMNIARGLFSEDDYLISEIMFNPF
                     TSGQLTKIREELKHLLEIVDAKDTDKLHTYLKKLRKNIE"
     gene            complement(6580..7644)
                     /locus_tag="PK641_00040"
     CDS             complement(6580..7644)
                     /locus_tag="PK641_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HPG54778.1"
                     /translation="MNDLKSILLPGVEDKRPLVISGPCSAETEEQVITTAKALADKKV
                     KIFRAGIWKPRTKPGGFEGVGVDGLSWLQEVKQETGMYVSTEVATSKHVEEALKAGID
                     MLWIGARTSANPFAVQELADALKGVDIPVLVKNPVNPDIELWIGALERIYNAGIRRLG
                     AIHRGFSTYDNKIYRNLPQWNVPIELRRRIPNMPIICDPSHIGGKRELIAPLCQQAMD
                     LSFDGLMIESHCNPDKAWSDASQQITPDILEYILNTLVIRETNQTTENLTELRKQIDA
                     IDNELLDLLSKRMRVSREIGTYKKEHSMPVLQSARYDEIMQKRVILGKSLGMSADFVS
                     EFLKSVHEESVRQQIDIINN"
     gene            complement(7684..8847)
                     /locus_tag="PK641_00045"
     CDS             complement(7684..8847)
                     /locus_tag="PK641_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPG54779.1"
                     /translation="MIQPAKRLSNVSEYYFSKKLKQIAEMNAAGLDIISLGVGSPDMP
                     PSEATIQTLCDQAKKADVHGYQPYTGIPELREAFAEWYKRWYGVTLNPKNEIQPLIGS
                     KEGILHVTLAFVNPGDKVLVPNPGYPTYTSLSTLLGADVIPYDLKEDNNWQPDFDALE
                     TMDLSGVKLMWTNYPNMPTGANATMQLYEKLVNFARKHQIVIVNDNPYSFILNKKPLS
                     LLSVEGAKECCIEFNSMSKSHNMPGWRIGMLATNATFVSWILKVKSNIDSGMFRPMQL
                     AAATALTNENEWHEAMNYDVYAKRRALAGQIMDELECKYDDSQVGMFLWGKIPDSYSD
                     VEVLTEKVLNEARVFITPGFIFGSNGKRYVRISLCAKEEKLQEALNRVKSIKK"
     gene            complement(8861..9703)
                     /locus_tag="PK641_00050"
     CDS             complement(8861..9703)
                     /locus_tag="PK641_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HPG54780.1"
                     /translation="MKRKIAIQGVAGSFHDIAAKNYFKGDELDIIPCGTFKEVFATIK
                     KDNSVIGVIAIENTIAGSLLPNHNLLKDSGLRIMGETKLRIEHNLVCLKGQTIDDVHD
                     VYSHPIALMQCEDFLAQHPHLCAVESDDTAQSAKRIAENNIMGRAAICGKLAADIYGL
                     EILASGIETNKRNFTRFLIIGDPWTADEHLKGKTVDKSSMVFALPHEEGSLAKVLSIL
                     SYYNINLTKIQSLPIIGREWEYLFYVNLTFDNYLSYSQSLDAIRPFTKDLRILGEYVG
                     SKEQ"
     gene            complement(10069..10398)
                     /locus_tag="PK641_00055"
     CDS             complement(10069..10398)
                     /locus_tag="PK641_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG54781.1"
                     /translation="MKNKRLYDILFGLAAFFILTGAVINLYYPQYAPYIFSVGVALLI
                     IVRINTMYKGNDFRLKRLQNMQIIATLLYIPAAYFMFVQSKVWVAILTAAAVMELVTS
                     FRAPSKK"
     gene            complement(10388..10696)
                     /locus_tag="PK641_00060"
     CDS             complement(10388..10696)
                     /locus_tag="PK641_00060"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HPG54782.1"
                     /translation="MKKSLQTILQIIFNKYLIVLVLFAVIMVTSENHNLLHRYKNKKT
                     IESLEKQISYYKNETAENKEKLDELRSDSSNLEKYARERYQMKKVDEDVFVIIQENEK
                     "
     gene            10758..11846
                     /locus_tag="PK641_00065"
                     /pseudo
     CDS             10758..11846
                     /locus_tag="PK641_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005802867.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
     gene            12010..12441
                     /locus_tag="PK641_00070"
     CDS             12010..12441
                     /locus_tag="PK641_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG54783.1"
                     /translation="MSLRGLLNRQESKNKPEQKKIEKIRLKEFTTDELIAAWYSFPNK
                     ISEQPKLHYLFKQIPLVDEEKNITIKVPNSIVDSEVKELIPTLHKHLADALDNDTIAI
                     STELMEQQSVLPTSSKEKARYMAEKNHELRKLFKDLKLDFI"
     gene            complement(12448..13149)
                     /locus_tag="PK641_00075"
     CDS             complement(12448..13149)
                     /locus_tag="PK641_00075"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HPG54784.1"
                     /translation="MHLFYAPDIEKTMCLPEDESNHAVKVLRLEAGNDIVILNGRGTM
                     YNATIAVAHPKHCVVNSLTPNKVKSTRDYYLHIAIAPTKNMDRLEWFVEKAAEIGIDE
                     ISPVFCRFSERKNLNNSRLERILVSAMKQSMQPFITKLNEPRQLSDLLKTAEADQKFI
                     AHCHEADKRLLSKSVEKAKSILILIGPEGDFSDEEVATALSMGYNAVSLGENRLRVET
                     AALVACHTVCLINEL"
     gene            complement(13153..13848)
                     /locus_tag="PK641_00080"
     CDS             complement(13153..13848)
                     /locus_tag="PK641_00080"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HPG54785.1"
                     /translation="MKYNKIGIIVAMTKEYELVKSTLQNTTEKEVHKFKFCTGLLGEK
                     KIILMKSGIGKVNAAVASIEMIEIYSPDCIINTGVAGGIDRNVNVADIVIGEQTTYHD
                     YYAGDVADHIKELGFPEIIPADSSMLNSLHTLAAKNNGIKFGLICTGDQFITNNVVLE
                     QIKAKHPFGLAVDMESNSIAHVCFLRDVPFLSFRIISDTPWVDNQNEQYLNFWESAPQ
                     KNFSLLSELIKNI"
     gene            complement(13857..15230)
                     /locus_tag="PK641_00085"
     CDS             complement(13857..15230)
                     /locus_tag="PK641_00085"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF002600.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HPG54786.1"
                     /translation="MKDLFYKRSHAASIIDDKDIDAAFVFCDKYKQFLDCAKTEREAA
                     TEIEKQLKAKKFKSYKYGDKIVAGDMIFMNNRAKSIIAAKIGKDSIDTGVNILVAHID
                     SPRIDLKQNPLYQNSEIALFKTHYYGGIKKYQWTTIALALHGVIIKSDGTKIQVNIGE
                     DEHDPVFCISDLLPHLAQEQMKRDASEIIKGEELNVMVGSLPFKNTKNAEKVKANILS
                     ILHDKYGIVEKDFLSAELELVPQNKAKDVGFDRSMIGAYGQDDRVCAYTVLQAFLDTE
                     TPQKTTIAVFADKEEIGSMGNTGMQSSILRYFIADIAEAFGSSERVVLSNSNCLSADV
                     NAAFDPTFPNVSEINNASLLNYGVVVTKYNGSKGKAGSSDANAEYISQIHAMLDKNKL
                     VWQTGELGLVDMGGGGTVAAYIANLGPQVVDVGVPVISMHSPFEITAKLDVYMAYKAF
                     CAFCKNR"
     gene            complement(15246..16667)
                     /locus_tag="PK641_00090"
     CDS             complement(15246..16667)
                     /locus_tag="PK641_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HPG54787.1"
                     /translation="MITFSFALLLLVVGYFAYGAFIERLFAPDASKTTPAILKTDGVD
                     YVPMSGWRIFMIQFLNIAGLGPVFGAIMGAKFGMSSYLWIVLGTIFGGAVHDYLAGMI
                     SIRNDGCNLPEIHGKYLGNQMKQFSRFFAVLLMILVGAVFVAGPAELLGSLTPSFFNT
                     TFWILIIFLYYFIATMLPIDKIIGKIYPLFAFALIFMAVGILVMIYIKFPALPEITDG
                     LANTHPGGESMPIFPMMFISIACGAISGFHATQSPLMARCMTNEKQGRRIFYGAMIAE
                     GIVALIWAAAATYFFQKNGMGISNAAEIVNDITKDWLGTFGGLLAILGVIAAPITSGD
                     TALRSARLMISDMFNFNQKPIKNRLLISIPLFLVTAGILLYSLRDKEGFNIIWRYFAW
                     ANQTLAVMTLWAITVYLLKEKKCYWVTLLPALFMTSVVVTYILVAPEGFGLSYYLSLS
                     IALSVVLLLLGLFFYKKIKNKVD"
     gene            16929..17180
                     /locus_tag="PK641_00095"
     CDS             16929..17180
                     /locus_tag="PK641_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HPG54788.1"
                     /translation="MDIHSPIVLLVVGMGTVFVALLIIIYSGEGLIRLVNRFLPEDEK
                     PTVDNKQTASVSPKTAQAISAAVQIITNNKGMVEKIEKI"
     gene            17196..19037
                     /locus_tag="PK641_00100"
     CDS             17196..19037
                     /locus_tag="PK641_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005815278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HPG54789.1"
                     /translation="MSKKKEVKFCLMFRDMWQSAGKYVPRVDQLLKVAPSIVKMGCFA
                     RVETNGGGFEQVNLLFGENPNKAVREWTKPFNEAGIQTQLLDRGLNGLRMSPVPADVR
                     KLFYKVKKAQGTDIARTFCGLNDTRNIIPSIGYAKEGGMISQCSLCITFSPIHTVEYY
                     TNMAFELIEAGADEICLKDMAGIGRPVSLGKIVKNIKEKYPNIPIQYHSHAGPGFSMA
                     SILEVCKAGCDFVDVGMEPLSWGTGHADILAVQAMLKDAGFDVPEINMEAYMETRSLI
                     QEMMDDFLGYYIPSKNRLMSSLLIAPGLPGGMMGSLMTDLETNLESINKYKAKNGQAA
                     ITQDQLLIKLFNEVSYVWPKMGYPCLVTPFSQYVKNMALMNVMQMEKGKERWSMIADD
                     IWDMMLGKAGQLPGKLDKELIAKAKEQGLEFHNEDPQSNYPDALDSFRKEMKDNKWEL
                     GQDDEELFELAMHPQQYRAYKSGQAKADFEADLAKRKADRENCNGSLSLPQTVTVDVN
                     GEKFAVTIGANNGKETSATTTVSAAPGEGTALTSPLEGKFYLVKNASDATVKVGDFVK
                     KGQTICYIEAMKTFNAIAAEFDGVITEISMPIGATVSEDDVLMKIKA"
     gene            19042..20277
                     /locus_tag="PK641_00105"
     CDS             19042..20277
                     /locus_tag="PK641_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HPG54790.1"
                     /translation="MEEILNKIYEMTAISNIVADPRFLIMYALAFILLYLGIKKQYEP
                     LLLVPIAFGVLIANFPGGDMGVTQAAEDGTIKLINGEIVNIWEMSLPQIAHELGIMNF
                     IYYLLIKTGFLPPIIFMGVGALTDFGPMLRNLKLAVFGAGAQFGIFSVLLVASQFFTM
                     NEAASLAIIGGADGPTAIFTTIKLAPHLLGPIAIAAYSYMALVPVIIPLVVKLTCTKK
                     ELSINMKEQDKLYPSKTEIKNMRVLKILFPIVTTTIVAIIVPSAVALVGMLMFGNLVK
                     EIGANTSRLFDAASNSIMNAATIFLGLSVGATMTVDAFLNFTTIGIVIGGFFAFGLSI
                     FGGIMLVKIMNLFSKKKINPLIGATGLSAVPMASRVANEIALKYDSRNHVLNYCMSSN
                     VAGVIGSAVAAGILISFLG"
     gene            complement(20352..21218)
                     /locus_tag="PK641_00110"
     CDS             complement(20352..21218)
                     /locus_tag="PK641_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005776070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HPG54791.1"
                     /translation="MEESVKKQIIIAQISEITEYIIYRKLASIEKDSANKAVLEQIAS
                     EENKHYKILQKLSGASPKPNMLKVYWYVFCARTFGLTFAIKLMESGEQGASEKYGMYD
                     IPEIKALAKEEQVHEQRLIALINEERLSYMGSVVLGLNDALVEFTGALAGFTLALGDH
                     KLIALTGSITGIAAALSMASSEYLSTKSEGGDSDKHPIKASVYTGVAYLITVIALISP
                     FVFCSNVIISLVIMLLSALLIIAIFNFYYSVARGESFKKRFLEMAILSFSVAGVSFLI
                     GYLLKLFTGIEA"
     gene            complement(21229..23292)
                     /gene="metG"
                     /locus_tag="PK641_00115"
     CDS             complement(21229..23292)
                     /gene="metG"
                     /locus_tag="PK641_00115"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPG54792.1"
                     /translation="MQFKTKNMQKKFKRTTVTSALPYANGPVHIGHLAGVYVPADIYV
                     RYLRLKGEDVLFIGGSDEHGVPITIKAKKEGLRPKDIVDRYHSIIKKSFEEFGISFDI
                     YSRTTSKTHHEVASQIFKTIYDKGGFVEKTSEQYYDEEAKQFLADRYITGECPHCHNQ
                     DAYGDQCEKCGTSLSPLELINPKSAISGSKPVLKETKHWYLPLDKYENWLREWILDGH
                     KEWKTNVYGQCKSWLDMGLQPRAVSRDLDWGIPLPIENTEGKVLYVWFDAPIGYISNT
                     KELLPNDWEKWWKSEDTRLLHFIGKDNIVFHCIVFPTMLKAEGSYILPDNVPANEFLN
                     LEGDKISTSRNWAVWLNEYLVDFPGKQDVLRYVLTANAPETKDNDFTWKDFQARNNNE
                     LVAIYGNFVNRTLVLTQKYFDGKIPACGELTECDSATLKEFADVKKSVEDLLDVFKFR
                     DAQKEAMNLARIGNKYLADTEPWKLAKTDMKRVETIMYLALQITANLAIAFEPFLPFS
                     SQKLRNLLNMDSFNWNELGRTDLLKTGESVSQPELLFEKIEDDAIEAQIQRLLNIKQQ
                     NALNNFKPAEIKQQVSFDDFGKLDIRVGTILECVKVPKADKLLQFKIDDGMGGRTIVS
                     GIAKYYPNPEVLVGKQICFIANFESRKLKGVESQGMILSAEDSDGRLVVVSPSDIVRS
                     GVSVC"
     gene            23359..23796
                     /locus_tag="PK641_00120"
     CDS             23359..23796
                     /locus_tag="PK641_00120"
                     /inference="COORDINATES: protein motif:HMM:NF022838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-like domain-containing protein"
                     /protein_id="HPG54793.1"
                     /translation="MKKALILLVCTSFFSFAAYADNEDMITTDQLPVVSQQFIKKYFP
                     NETVAYAKYDPEFFDSAYEITFKSGNSIEFNKQGEWKDYECKTCIVPDELLPNEISSY
                     IKEKYPDKNIVSIERGKKTYEVKLSNGLELKFNLRGQLLEIDL"
     gene            23827..25377
                     /locus_tag="PK641_00125"
     CDS             23827..25377
                     /locus_tag="PK641_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B family protein"
                     /protein_id="HPG54794.1"
                     /translation="MEYILSHSWIRGQKCFVRGYCFDEQSNFIKSEKLLSLFSGIQTE
                     EDFSKKIKQLNGIFQVIIETSQGVLAAGDIAGTLPLYYRNYNNKCFLSDNVYSLLKGN
                     ETIDKNAETDYLNLNYTLNSKTLINGIHRIEPMTYASISNNSVKTSSYYSYRIYKNEI
                     NNIDIQYQFSVALLNIFGRLIEYINGRQVIVPLSGGYDSRLIVAMLYRLNYKNIICYT
                     IGDENSEEVEIAKSVAKSLGFKHYCIDNTLMAKKCDLSKDVDFLDYCKYVGQLCNKIW
                     TFDFFSVKWLKENGIAEYDAVFVPGHSGDCLAGSHSSKNHITQHSTASYMAVKVLEDQ
                     SFLGRNKELQNKITKLLQKKEKTLRTSNFDDFVIKNRLAKYINSATRVYSYFGFDIAL
                     PFWDIEMMDFFKTLDPSLKQNQKFYHNYLDSSLFGPLGISFKGRLQASDMDLRLHCIK
                     SFIKRMLPLKIAVSLSHTPDLVGMNDILKPFIKELAKVGIKTKTNNPNEIMALWYLYQ
                     VKQNLCAH"
     gene            25365..26636
                     /locus_tag="PK641_00130"
     CDS             25365..26636
                     /locus_tag="PK641_00130"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HPG54795.1"
                     /translation="MRALVNIKKLVSREFIRHTATLISGNIIAQAISLLIYPVLTRLY
                     SPDDFGVFNLFLSIGGCIVILSTGAFHYSIILPKQEKDSLTCVRLCAYCIIIATLISL
                     LPAFFKTFTADLFNTTDLADSLIYLPLFVLSGGLWSCINYWFIRQKSFKHIAEYQITL
                     NASNSGLKTVFGIIQSGYGLIIGTIIGQITAISISLIHCKKYFKSFFKYGNFSELRQT
                     ASKYADFPRYALPRNLINYFGSNLLVLLLSPYFGMAKIGIFGMAIALSMRPIILINNA
                     LSQTITQKISYCIKEHTSAVPMIKKYFKYSIPTIIVGFGVLYLVLPATCSWLLGETWR
                     DAGEYIRLMLPWLACLLWFYPFSCIPDILNKQRTDMLFEITITVVRVMAIIIGIAIND
                     FYVTILLYSLLSSLIIIIRGFWFFKLAKTCT"
     gene            26699..27229
                     /locus_tag="PK641_00135"
     CDS             26699..27229
                     /locus_tag="PK641_00135"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPG54796.1"
                     /translation="MKQNRLIWSLIGRIFYPHFKSKSYINSTLLKFVFWQKILGINRN
                     TPWPVHFTSKVICSEKIQNDNGFRAPGYAMGCYIDGRNGIIIESGSIVGPKVSIISQN
                     HNKSDYSRFDASNPIIIRKNSWLACGCTILAGVELGEHTIVAAGAVVTKSFLEGNCIL
                     AGNPAKKIKDIPPYNV"
     gene            27249..28220
                     /locus_tag="PK641_00140"
     CDS             27249..28220
                     /locus_tag="PK641_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG54797.1"
                     /translation="MNNRELYKQLCNTHPEIPLFMQYWWMEAACKPDSWNVLFCGDDD
                     NTEAVLVFHIQEKLGIKRVIQPTLTQYNGIWINYPKDISLDKRYDFENRVMENLINQL
                     DAMNLDFYEQKFHYSFTNWQPFYWKQYKQTTHYTYLIENIADRDVVFAMMSHKKRQKK
                     ILSSEDNFTLDTDMPINDFYEFCKNTIEKKQHAPILYTFDTLRKIYDASVERNQGMIF
                     TIRDKENNIHCSLFTVWDDNSAYNLIIAINPKYKSSGASSCIIWMVMDALKDKTLNYD
                     FEGSMIQGVALKNQSYGAKQIPYSVISKSSCKYNFIKSLRNLLHIGF"
     gene            28327..29013
                     /locus_tag="PK641_00145"
     CDS             28327..29013
                     /locus_tag="PK641_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HPG54798.1"
                     /translation="MKQYLLDLLRLVYPEICLVCGGYLVEGEKCICLECVHKLPKTGY
                     HLINDNMAEQLLWGRVKIEYGTALCRFQKGNSVQKVLHQLKYKGEKELGIVLGQFFGY
                     EIQGSNISQVDAIIPVPLHPVKLKKRGYNQSEQIAIGLSEVLNIPIVNDLLYRKLANS
                     TQTKKNVYERWENSQNIFDIKENSLSLCEKHILLVDDVITTGATIEACTEVLQKIKGL
                     RVSFAALAIA"
     gene            29024..29761
                     /locus_tag="PK641_00150"
     CDS             29024..29761
                     /locus_tag="PK641_00150"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HPG54799.1"
                     /translation="MTTNFLTHLDEIESTNEWMLQSLKQNKNLNHGDMVMADFQSNGR
                     GQMNNHWESERGKNLLCSIILFPKRMQANAQFPISEAVALAVADYLDIRSSFISVKWP
                     NDIYWNDKKIAGILIENNLSGNYLSDCIVGIGLNLNQSEFKSDAPNPISLNTITKRFF
                     DPKTAAKRIRIHLMSRFWQAENTPDILHEEYLKKLYRRDIAATYKDANGTFIGVITDV
                     ETTGHIVIKDESGNLRRYAFKEVQFVL"
     gene            29877..30338
                     /locus_tag="PK641_00155"
     CDS             29877..30338
                     /locus_tag="PK641_00155"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPG54800.1"
                     /translation="MRKNSIIITLILTFTINVLAADEGLVIINQDNRLDQLVHQNTGI
                     SQLPANNAISVSGYRVQLYSGNNPQTAKQEALKLEKELTEFYPEIKSYVTYNAPFWKV
                     RIGDFTSYYEALLFSRRLKVDFSRFENEIYVMKEDAVKPIYFENNGLNLIP"
     gene            30335..31357
                     /locus_tag="PK641_00160"
     CDS             30335..31357
                     /locus_tag="PK641_00160"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HPG54801.1"
                     /translation="MKKTFLIITSALCILFASCSVVEPDPSLDDKDLVEGYKFSQTIS
                     INYGEDGEPQITNPLSGQGVEITNNGQHVTIRSTMTDPVCYELKGTCTEGSVKIYGEE
                     KFKLSLNGLDMSSTDSCAINIQSSKRCFLVLEDGTTNKITDGAGYPTDKEELTSGGED
                     KKGAFFSEGQVIVSGNGTLELRGRNKHALATDEYLRIRSGNITITNAASDGLHVKEYY
                     WQEGGEINVNNQNDGLQCSEGYIKIDSGILNIVTADDGILTNYGDEPTDTEAIDASIT
                     INGGDINIIAMSGKGMYSNGNIYLNAGKLTITCIKKEKVISTNPTKTASIILVDTETH
                     YTPAQD"
     gene            31366..>31912
                     /locus_tag="PK641_00165"
     CDS             31366..>31912
                     /locus_tag="PK641_00165"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPG54802.1"
                     /translation="MLIRLYEENPNSKEIERIVGILKDGGIIIYPTDSLYAFGCDITN
                     AHAVEKICKIKNIDPGKMPLSFVCSNISHISQYSQLDNNTFKLLKECLPGPFTFLLNG
                     NSNLPKLFKKKKTVGVRIPDNGIALALVEALGNPILSTSIFIDEDEPEYSTNPELIEE
                     NYAGQVDCVIDGGGGSLEPSTI"
BASE COUNT        10608 a         5716 c         5132 g        10456 t
ORIGIN      
        1 tacaccatta tcagctccca atgttgtgcc ttgagcaaaa attttaccgt ctttttcaga
       61 atattttata gaatcttttg taaaatcgtg aattgtaccc ggcgcaattt gtggcaccat
      121 atcaacatgt ccttgtaata ttatggcagg acgtgattcc atacctatag ttgccggctt
      181 tcgtataaca atatttcctg cactatctat acttgagtca aaattatttg ttttagcaaa
      241 ttgttcaata tgcttgatta tagcactgca gtttcctgag ggacgtggta ccttacatat
      301 ctcatcaaag tatttccaaa acaaagttgt actagtcatc tttatgttgc aattatagtt
      361 cgctctccca aatagcgagc caaagtctgt ctgtatttat tcaattcatt ttgcttttca
      421 agcaattcat ttatcttttc atgatcatgt tccgcattag cttgtttcaa tgctgctatc
      481 aactcattac tttgtttctc gagtatttta tttttgtatt caagagtaat tctaggtatt
      541 ataagtctca acctttcctc ttcagtttct gttttattat atttatcatg aatattactt
      601 cgttcatatt tatcttcaga taaataaaaa gcgtaatttc ttacagattc atcaggatga
      661 ttaataaaat aatggcgaag atcgccttct accacaatcg cttcttgtag aatgtgctta
      721 tgtaaaccgt attccaattt catattatca gcaattaaac tctgattaat ataatcaagt
      781 acagaaatct gctcaatttt attttcgtct acctctaccg gtataagatt tttgccgtat
      841 ctgattatat atcttataag attttgctct tgcataaatg caaaaggctt ttcttgctct
      901 ttttcaacta cagcctgttt tatttcattt tgagcaatag ttttttgaat gcctgatatt
      961 ttctttgcca atacttcagc taatactgat tcacgcattt caaaacgatc tgcgcactct
     1021 tttatatata aagaacgtat tattgtatcc ggtataacag atatgctggt tacaatatcg
     1081 ttaattaaat tggctttttt tattggatct cctgcagcat catctaataa tagttgagtt
     1141 ttaaatctga taaaatcagt ttgatgctct tttataaatg cattaaattc gcttgaatct
     1201 ctacttctgg caaaagagtc aggatcttca ccatcaggaa gtaaaaccac ttttacattc
     1261 atgccttcag acaaaagcat gtctattcct ctaattgatg catgtatgcc tgctgcatca
     1321 ccatcataca gaactgttat gtttgaagta aatctatgaa ttagtcttat ctgaccgggt
     1381 gttaatgatg tccctgaaga tgcaaccacg ttttcaattc cggcttgatg catcgaaata
     1441 acatcagtat agccctcaac taaaaaacaa cgatcttctt taacgatact attttttgca
     1501 aaaaatattc catatagttc gttactttta tgatagattt cagactccgg tgagtttaca
     1561 tactttgctg cattgtctgt ctttttaagt atacgaccac caaatgctac aatttttccg
     1621 gaaagagaat gtacaggaaa catcacccta cccctgaacc tatcaactaa gccaccgcgt
     1681 tcagattcgg tacataatcc tgtttttagt aaaaactgtt tgttataacc acttttaatt
     1741 gcttctttgc tcaaagaatc gtatttatct aaacaaaatc caagttgaaa tttctttata
     1801 atatcatctc taaaacctct ttctttgaaa tacgatatcc ccagagccct tccgtcttcg
     1861 ttttcccata agattttagt aaaataagtt aaagcgaact gattaattgc aaacatactc
     1921 tccctgtcat tgcgatgctc aatctcttca gaagacaact ctttttcgat aatctctata
     1981 ttgtattttc gagctaaata acgtaaggca tctgcataat ccaattgctc atgcaacata
     2041 ataaaattaa caggtgaccc gccttttgaa catccgaaac acttgcaaat attttttgcg
     2101 ggagatactg taaatgaagg tgttttttca ttatggaaag ggcatagacc aattagatta
     2161 acacctcgtt ttttaagtgt tacaaagtca cctacaacct cctctatttt ggctgcagtt
     2221 aatattctat cttttgtagc ttgatctatc ataataaaac aaagataaga tttttttatg
     2281 accctataac aataattatt ttgttaatct actgttgtaa tcttttgatt tcaccggatt
     2341 atcattcaac agatttcaga tgagttccac gtaataaatc ctgcacgtcc atagtttttt
     2401 tacctgctaa ttgtaaacga tgcacttgta catagccgtc agaagcagta aacttcaaat
     2461 atgttttatt gtcagttaat aaaacacctg atggttctga tatttttgtc tcttcaactg
     2521 atacatcata aattttaaga ctgagaaccc tatctttctc gtcttttact tctgtaaaag
     2581 cagcaggata aggagataaa cctctaacaa aatttttaat ctcaatcacc gacttactcc
     2641 aatctattcg gcaagtatct ttaaatattt taggtgccgg ttttaaaaca attgattcat
     2701 cttgaggtgt taaaatataa tttccggaga ttataagatc aacagtttct gttactactg
     2761 atgcacctaa gtacattaat ttatcgtgta tatctcctac attctcatca tcttctatag
     2821 agaccttgct ttgcataatt atattacctg tatcaatctc atgttttaaa aaaaatgtag
     2881 ttacaccggt ctctttatct ccatttatta ctgcccaatt aattggggca gctcccctat
     2941 attgtggtaa tagagatgcg tgaagattaa acgtaccata tttgggcata ttccaaacaa
     3001 cttcaggtaa cattctaaaa gctacaacaa tttgtaaatc tgcattccac tcgcgtagag
     3061 atgtcaaaaa accttcatct ttcaagtttt caggttgcaa taatggtaaa tttctttcaa
     3121 gtgcaaactt cttaactgca gattgttgca attgataccc tcgccctgcg ggtttatcag
     3181 gcattgttac aacacctaca acattgtatg agtcttcaac caatttcttt aatggttcaa
     3241 ctgcaaaatc aggtgtaccc atgtacacta tacgcaatgt ctttttttcc atttctgcta
     3301 ttaaatgggt agagacgtac cttggcacgt ctctactcgt atttattaca attaaagttg
     3361 attatttctt ttcattcaga agctctaata tctcattggt gatattatat gctttattac
     3421 catatagaat attgttacta ccggtattac taaaaataaa atgatatttc ttgtctttgt
     3481 tataaacggt aactacagaa tcaagtgttt tgaataattt atcattcata tttttttgtt
     3541 ttacaacaag atcttgggcc aatttgttgt caagctcttg taattcgcgt tgcttgcgaa
     3601 ccaaacgttc ttgttcgcta cgtgcgcgtt cttctgacaa aaatgctctg ttctctattt
     3661 tattctgaaa atcaaccatt tcagcttgta aagcattagc tttttgatta acacttgcac
     3721 gagaacgttc ttcttcttgt agtaactctt cgctcattga tttatataaa tcataattga
     3781 gcaacaatgt atcaatattt acgtatgcta ttggtaaaac ttcaccttcg gttaaagcct
     3841 gttcttttga attctcttct ttattgataa agaataatat aaacaagact acaactgcaa
     3901 cgactaatac gccattaata atgtaaagta ctaaattttt catgctttta tttgatttgt
     3961 tttttgtttt ctagcctctt tatcttgtgt ttgcacgcaa tgaattcctt tttctcttaa
     4021 tgcagcattc gaatctacat gaagacccgg aaatttaccc ttctgtgtaa aaaacacacg
     4081 ataccccatc aataaaattg ctggtatcaa aataactaga gacaacaaaa aggctttaag
     4141 catacattgt ttaataatga ggagcaaata taggtcataa aaaaattaat tgtgaaacat
     4201 cacacataat tttgtacttt aataacgaaa tttttaacgt tttcatcttt tttaaaaact
     4261 gcgctataat tattacctaa atactatgtc ctttttaaag tttaattaca taataaagac
     4321 ttaaatttat ttacttttgc aatatataaa ataagccatt tagatataaa catttacaat
     4381 gaatcaaaat atgccgaacc ctattgatgt gtggaaatat ttttctgaga tatgcaaaat
     4441 tccacgtcca tcaaagcacg aagaaaagat tactgcttat ttaattcgat ttgcacaaaa
     4501 aaataatctt gaatacaaac aagatgaggt aggaaatgta ttgataaaaa aagaaggaat
     4561 cagccctact gtaatattac aagcacacac tgacatggtt tgtgaaaaaa acgacaatgt
     4621 gattcatgat tttatgatag accctatcaa ctttactcaa gatggagatt ggatttctgc
     4681 aaatggtact accctgggag ctgacgatgg aattggtgtt gcaatgatgc ttgcattatt
     4741 agcaggtaat gaaactcaac gttcgcttga atgtttattc acaattgatg aagagaccgg
     4801 acttacagga gcatcagcac taaagaatga ttttatttcg gctaattcgc taataaattt
     4861 agactctgaa acagatgatg aaattattat aggctgtgcc ggtggatgcg actcaattct
     4921 aacttttaat atcgaaaaag agcctatgcc ggagcattta ttcgcaatat ccttaaaaat
     4981 tttcggatta caaggaggac atagtggaga tgacataaac aaagatcggg caaatgcaaa
     5041 taaactactg aacgaatttt taaacttagc atctaaacaa tttgatttat cagtatgcaa
     5101 tattaatggt ggtggactaa gaaatgcaat tgcgcgtgaa gcaagtgcta caatagcagt
     5161 tccatgggct gacaaagaaa gaatacgaat agcatggaat attttcagtg cagaaactga
     5221 ggataattgg ataaaaaaag agcccggcat gaaattatca cttgattctt gcaaagtccc
     5281 tgacacaaga attaaacatg atgatgcgct gaacattatt aatgcaataa gcgaatgccc
     5341 acatggtgtg ttagaataca gcagtgagat tccgggactt gttaagacat ctacaaatct
     5401 tgcatcaatt aaaacattag aaaattctat agttatatgt acaagtcaac gcagcgacga
     5461 tatagataaa ttacataata cggcatctat gattgataaa actgcgaata agcatcacgc
     5521 attagttaca cactcaaacg aataccccgg ttggaagcca aattttgaat ctcctttggt
     5581 aaaaaaggct gatagtatat acaagcaact ttttaaaaag gctccaaaga taaaagtgat
     5641 acatgcaggc ttggaatgtg gtcttttctt gcaaaaattt cccaacttgg acatgatttc
     5701 aataggacct aacttggaag gagtccattc gcccgatgaa cgcgtttctg tttcatcggt
     5761 cgaaaagaca tggagatact tgattgaact cctaaaataa atttactcta tatttttgcg
     5821 aagtttcttt aaataagtgt gcagtttatc agtatccttc gcatcaacta tttctaaaag
     5881 atgtttgagc tcttcgcgaa tttttgtcaa ctgccctgaa gtaaatggat taaacatgat
     5941 ttccgaaata agataatcat cttcagaaaa caatcctctg gctatattca tgtgtttctt
     6001 aaatgttgta cccggagctt cttggtgttt cattacggca gcaaacacca tagtagatgc
     6061 aaatggtatt gacaatgaat atgcgattgt ttcgtcatgt tcaaggaatg tatattcaaa
     6121 tattttaagc ttaagtgagc catataaatc tttaaagaaa gccttgccca tgtgatctga
     6181 ctcggatatt atgattgcac tctgattgct tagatcttct aaatttgcaa atgtaggtcc
     6241 aaacataggg tgcgttgata cataacgcat tccacattgt ttgtaatatt cagggagtcc
     6301 cgttttaaca gatgctatat ctgaaataat acaagtctta ggtaaatatt gtatcactgc
     6361 attaaatgca tcaatagtat atttaagcgt tacgcagtta atcattaatt caggttcaaa
     6421 ctcttttatt tcctcgtatt ttgtcattcg atatgtattg aacacaaatc gaagattttt
     6481 aggattaagg tcgaataacg ctacctcatg ttgaacgcac aaaacatctg ttaaaaaggt
     6541 gcccatttta cctgcaccca aaattaatat cttcatcgtt taattattaa tgatatcaat
     6601 ttgttgacgc actgattcct catgtactga ttttaaaaac tcacttacaa aatctgctga
     6661 cattccaaga cttttgccta gaataacacg tttttgcata atttcatcgt aacgagctga
     6721 ttgcaaaaca ggcattgaat gttccttctt atatgttcct atttcgcgag aaacacgcat
     6781 acgttttgac aataaatcaa gcaattcatt atcgattgca tctatttgtt tacgtaattc
     6841 tgtcaaattt tcggttgtct gatttgtttc acgaattact aatgtattca aaatatattc
     6901 caatatatca ggagttattt gttgagatgc atcactccaa gctttatcag gattacaatg
     6961 tgactctatc atcaatccat caaaactcag atccatagct tgttgacaaa gtggtgctat
     7021 aagttcgcgt tttccaccaa tatggcttgg gtcacagata attggcatgt ttggaatacg
     7081 acgacgtagt tcaattggga cattccactg cggcagattt ctatatattt tgttatcgta
     7141 agtactaaaa cctctatgaa tagcacctaa tctgcgaatt cctgcattat agattctctc
     7201 taaagctcct atccataact ctatatcagg gttcacagga ttttttacta atacaggtat
     7261 gtcaactcct tttaaagcat ctgccaattc ttgcactgca aatggatttg cagatgtacg
     7321 agcgcctatc cataacatat caattcccgc ttttaaggct tcttctacat gttttgaagt
     7381 agcaacctcg gtacttacat acattccggt ttcttgttta acctcttgta accatgataa
     7441 accatccaca cctacacctt caaaaccacc gggttttgtc cttggtttcc atattcccgc
     7501 acgaaatatt tttacttttt tatcggccaa ggctttggct gttgtaataa cttgttcctc
     7561 ggtttctgca ctacatggtc cggaaataac tagaggacgt ttgtcttcta cacccggaag
     7621 taaaattgat tttaaatcat tcatattttt tatatttgtt tgttttataa tttctgttac
     7681 caattatttt ttaatagact ttacacgatt taaagcttct tgaagttttt cttctttagc
     7741 gcataaagat attcgcacgt atcgcttacc attactaccg aatataaatc cgggagttat
     7801 aaatacacgt gcttcattta atactttttc ggtaaggact tcaacatctg aataagaatc
     7861 aggaatcttt ccccataaaa acataccaac ttgagaatcg tcatatttac attccaactc
     7921 atccattatt tgtcctgcta atgcgcgacg ttttgcataa acatcatagt tcatagcttc
     7981 gtgccattcg ttttcatttg tcaaggcagt agctgcagct agttgcatgg gtctgaacat
     8041 tcctgaatct atattgcttt ttacctttaa tatccaactt acaaatgttg cgttagttgc
     8101 caacatacct atacgccaac ccggcatatt atgactttta ctcatcgaat taaattcaat
     8161 acaacattct tttgcacctt ctacagacaa taaactaaga ggttttttat ttaaaataaa
     8221 actataagga ttatcgttta caattacaat ttggtgtttg cgtgcaaaat ttactagttt
     8281 ctcatataat tgcattgttg cattagctcc ggttggcata ttaggataat ttgtccacat
     8341 caacttaaca ccgcttaaat ccattgtttc caaagcatca aagtcaggct gccaattatt
     8401 atcctctttt aaatcataag gaatcacatc tgctcctaac aatgtactta aagacgtata
     8461 agttggataa cctgggttag gtactaaaac cttatctccc ggattaacaa atgccaaagt
     8521 tacgtgaaga ataccttctt ttgaacctat taatggttga atttcgttct ttggattaag
     8581 cgttacacca taccaacgct tataccattc tgcaaatgct tcgcgcaatt cagggatacc
     8641 tgtatatggt tgataaccat gaacatcagc tttttttgct tgatcgcata atgtctgaat
     8701 tgttgcctca gatggtggca tatccggact accgactcct aaactgatga tgtctaatcc
     8761 ggcagcattc atttcagcaa tctgttttag tttttttgaa aaatagtatt ctgaaacgtt
     8821 cgacaatctt tttgcaggtt gtatcataat gattagtatt ttattgttct tttgatccta
     8881 catattcacc taatatacgc aagtcttttg taaagggtct tattgcatcc aatgattgac
     8941 tatatgataa atagttatca aatgtcagat taacatagaa caaatattcc cattctctgc
     9001 cgattattgg tagtgattgt attttggtta agtttatgtt atagtaagac aatattgaca
     9061 aaacctttgc taaactacct tcttcatgag gcaaggcaaa taccattgat gatttatcca
     9121 cagtttttcc tttgaggtgt tcatcagcag tccaaggatc gccaataatt aaaaaacggg
     9181 taaagttcct tttattagtc tctatacccg atgctaaaat ctcaagtcca taaatatctg
     9241 ctgctaattt tccgcaaatt gctgctctac ccattatatt attttcggct atccgcttag
     9301 cgctttgtgc agtatcatca ctttctactg cgcaaagatg aggatgttgt gccaagaaat
     9361 cttcacactg catcaaagca attggatgtg aataaacatc atgaacatcg tcaattgttt
     9421 ggccttttaa acaaaccaaa ttgtgttcta ttcgtaattt tgtttctccc atgatacgca
     9481 acccactatc tttcaataga ttgtgattgg gaagcaagct tccggcaata gtattttcta
     9541 ttgcaataac tcctattaca gaattatctt ttttaatggt cgcaaatact tccttaaaag
     9601 taccgcatgg tattatatca agttcatcac ccttaaaata attttttgca gcgatgtcgt
     9661 ggaaagatcc tgctactccc tgaattgcaa tttttctttt cataaatgtg tgtttaaaaa
     9721 aaagaatccc gaccttacgg acgggattct tatatatttt agaatgattt ttatttcata
     9781 ttacatacat taaatcccgt tcacttcgtg ctaaagtaaa agtaaaaata aaagtatgta
     9841 aaagatatat ttctcatttg ttctcaataa ttatgggtca aaggtaagga ttatttttaa
     9901 atatgaaaca tttattcaat aaaaaactac aaattaataa tatgtacctt aaatagtttc
     9961 aatttaacaa gcaacgatgc ctattctaat gattcattat ttacggttgt caagaaatta
    10021 agcatcaatg tgtatgactt ttgacaataa gcattaacaa tcatgatgtt attttttaga
    10081 aggagctctg aaagacgtaa caagttccat aacagccgca gcagtaagta ttgctaccca
    10141 cactttactt tgaacaaaca taaaataagc tgcaggtata tataataatg tggcaatgat
    10201 ttgcatgttt tgcaagcgtt taagacggaa atcattgcct ttatacatag tattaatacg
    10261 cactataatt aacaaggcaa caccaactga aaatatatat ggagcatact gcggataata
    10321 taaattaatt actgcacccg ttaaaataaa aaacgcagcc agcccaaata atatatcgta
    10381 taaacgttta tttttcattt tcttgtatta ttacaaacac atcttcatca actttcttca
    10441 tttgataacg ctctcttgca tatttttcaa gattactgct atccgaacga agttcatcca
    10501 atttttcttt gttttcagca gtctcgtttt tgtagtaaga tatttgtttt tcaagcgatt
    10561 caattgtttt cttattttta taacgatgga gcagattgtg attttcgctt gttaccataa
    10621 ttacggcaaa caaaactaat acaatcaagt atttgttgaa gataatttgt aatatcgttt
    10681 gtaaactttt tttcatttct gcaaaggtac agaatttgtt tttaatttat tatctttgcc
    10741 aatgtaaaaa aagaaagatg gataattact tggtatcggc acgaaaatac agacctataa
    10801 catttgacac tgttgttggc caagcgccgc tcgtcactac attaaagaat gcaattaaaa
    10861 gcggacaatt agctcatgca tacttatttt gcggttcgag aggggttgga aagaccacca
    10921 ccgcacgcat ttttgcaaaa accattaatt gttttaatcc tacaaaagac ggagaagctt
    10981 gtggcgaatg cgaatcttgc aaagcatttg ctgatggcag atctttcaat attcacgaat
    11041 tagatgccgc atcaaacaat agtgttgacg atataagaaa tttgacagat caggttagaa
    11101 tcccgccaca aataggtaaa tacagcgtgt acattattga tgaggttcac atgcttacat
    11161 cgtctgcttt taatgccttt ctgaaaacct tggaagaacc gccgtcatat gctattttca
    11221 tcttagcaac taccgaaaag cacaaggtaa ttgctactat attatctcgt tgccaaattt
    11281 atgattttaa tcgcatcagt gtaaaagata cagttgcgca tttaaaagac attgcagcca
    11341 aagaaggagt tgaaatagat gacgaagctt taaatgttat agcattaaaa tctgatggag
    11401 gtatgagaga tgcactttct acattcgacc aggttgtaag cttctgtggt tcaaaagtcg
    11461 actatcatag tgtaattgag aatctaaaca tacttgatta cgattattat tttcgttcga
    11521 ttgatttatt cttaacaaac aatgttcctg ctgccttatt acttttcaac gaaatcttag
    11581 acagaggatt tgatgcacat aattatgtta ccggtttgtc agaacattta cgtgatactt
    11641 tagtttgcaa agacaaaatt acggcagatc ttctagaagt cggttccagc atcagagaca
    11701 aatacataga acaagcttca agatgttcgg tcgatttttt gtatgatgcc ttagaagttt
    11761 gtaatgattg tgatatttct tataagcaga gcaaaaataa gcgtttaggt gttgaactaa
    11821 tgttaatccg attgtgccaa ctatcggggc aaaaaaaaaa ttaggcgact aagaaagaaa
    11881 aatccgctac ttcccttctt cccttccgaa ttcaccaaag ctaagtctgt tgcatcaaat
    11941 atcagtccac aaatcaacaa gacaaatgag gcagcaccat ctgcatcaga tattaaagtt
    12001 gctcctataa tgtcgttacg tggtttactc aataggcaag agagtaaaaa caagccggaa
    12061 caaaagaaaa tagaaaaaat acgtttaaaa gaatttacta ctgacgaact tattgcagca
    12121 tggtattctt ttccgaacaa aatatcggaa caaccaaagt tacactatct gtttaaacaa
    12181 atcccgttgg ttgatgaaga aaaaaatata acaataaagg ttcctaatag cattgtcgat
    12241 tcggaggtta aagaattaat accaactcta cacaaacacc ttgcggatgc acttgataat
    12301 gatactattg caattagcac cgagctaatg gagcaacaat cagtattgcc aacctcatca
    12361 aaagagaaag cacgctacat ggctgaaaaa aatcatgaat taagaaaatt atttaaggat
    12421 ctgaaattag acttcatttg attaagtcta taactcatta attaagcaaa ctgtatggca
    12481 tgctactaat gcagctgttt ctacccttaa tctattctca ccaagcgata cagcattata
    12541 tcccatagat aatgcagtag cgacttcttc gtcactaaaa tcgccttccg gacctattaa
    12601 tatcaatatc gatttcgctt tctctacact ttttgaaagc aagcgtttat cagcttcatg
    12661 gcaatgagct ataaactttt gatccgcttc tgcggtttta agcaaatccg acaactgacg
    12721 cggttcattt aatttagtaa taaatggctg catcgactgt ttcattgccg aaacaaggat
    12781 tctttctaaa cgactgttat taagattttt tcgctctgaa aaccgacaaa aaaccggaga
    12841 aatttcatct atgcctattt ctgcggcttt ttctacaaac cactcaagtc tatccatgtt
    12901 tttagttgga gcaatggcta tatgcagata gtaatcgcgc gtagatttta ctttattagg
    12961 tgttaatgaa ttgacaacac aatgctttgg atgagcaact gcaatagttg cattatacat
    13021 agtgcctcgt ccattaagta ttacaatatc attgcctgcc tccaaacgta aaaccttgac
    13081 agcatgattt gattcatctt caggcaaaca catcgtcttt tctatgtcgg gagcgtaaaa
    13141 caaatgcatt tatcaaatat tttttattaa ttctgaaagt aatgagaaat tcttttgtgg
    13201 agcactctcc cagaaattta aatattgctc attctgatta tcaacccatg gagtgtcact
    13261 tataattcta aaactcaaaa atggaacatc acgcaaaaaa catacatggg caatactatt
    13321 agattccata tcaactgcca aaccgaaagg atgttttgct ttaatttgct ctaaaactac
    13381 attattggtt ataaattgat cacctgtgca tattaagccg aacttaatgc cattattttt
    13441 tgctgctaat gtatgcaaac tatttagcat agaggaatct gccgggataa tctcaggaaa
    13501 acctaattct ttaatatgat cggctacatc acctgcataa taatcatgat acgtagtttg
    13561 ttctcctatc acaatgtcag ccacattcac atttctatct atacctccgg caactccggt
    13621 atttattata caatcaggag agtatatctc aatcatttcg atggatgcaa ctgcagcatt
    13681 aacttttcct ataccacttt tcatcaaaat aattttcttt tcgccaagta atcctgtaca
    13741 aaatttaaac ttgtgtacct ctttttcagt ggtattttgt aaggttgatt ttacaagttc
    13801 atattctttt gtcatggcaa cgatgatgcc tatcttatta tatttcataa ttaaatttat
    13861 ctgtttttgc aaaatgcgca aaatgcttta taagccatat aaacatcgag ttttgctgta
    13921 atttcaaatg gagaatgcat acttattaca ggaactccga catctacaac ttgagggccc
    13981 aaattagcta tataagctgc tacagtacca ccacctccca tatctaccaa tcctaattcc
    14041 cctgtttgcc aaactaattt atttttgtca agcattgcat gtatttgcga aatatattct
    14101 gcattggcat cagaagagcc cgccttccct ttacttccgt tatatttagt aacaactacg
    14161 ccataattga gtaatgatgc attattaatt tctgatacat ttgggaatgt cggatcaaaa
    14221 gctgcgttta catctgccga taaacaattt gaatttgaca gtacaactcg ctccgaactg
    14281 ccaaaagctt ctgcaatgtc agcaataaaa taacgtagaa tagatgattg cattcctgta
    14341 tttcccatac tgcctatttc ttctttatcg gcaaacactg ctattgtggt tttctgtggg
    14401 gtttcggtat ctagaaatgc ttgaagcaca gtatatgcac aaactctgtc atcttgtcca
    14461 tacgcaccaa tcatactgcg atcaaaacca acatcttttg ctttattttg aggaacaagt
    14521 tccaattcag ctgacaagaa atctttttct acaataccat acttgtcatg taatatagat
    14581 aaaatatttg cttttacttt ttctgcattt ttggtatttt taaagggaag agaacctacc
    14641 atcacattta gctcctcacc cttaataatt tctgaagcat cacgcttcat ttgttcttga
    14701 gctaaatgag gcaatagatc acttatacaa aaaacagggt catgttcatc ctcacctata
    14761 ttaacttgaa ttttagtacc atctgacttg ataataaccc catgtaaagc aagagctata
    14821 gttgtccact gatatttttt aatacctccg taataatgag tcttaaacaa tgctatttca
    14881 gagttttggt ataaaggatt ttgttttaaa tctatgcgag gcgaatctat atgtgcaact
    14941 aatatattaa cccctgtatc aatagaatct tttcctattt ttgccgcaat gattgatttt
    15001 gctctattat tcataaaaat catatcgcct gcaacaatct tatcaccata tttatatgat
    15061 ttaaattttt ttgcttttaa ttgcttttct atttcagttg ctgcttctcg ttctgttttt
    15121 gcgcaatcca aaaactgttt atacttatca cagaacacaa atgcagcatc aatatcttta
    15181 tcatcaataa tacttgctgc atgcgaacgt ttataaaata aatctttcat gttaatatat
    15241 ttactttagt cgactttgtt ttttattttc ttatagaaga acaatcctag taacaataat
    15301 actactgata aggctattga caaactcaaa taatatgata atccaaatcc ttcaggtgct
    15361 actaatatgt atgttacaac aactgatgtc ataaataatg ccggtagcaa tgttacccaa
    15421 taacatttct tctcttttaa taaatacact gtaattgccc ataatgtcat tacagctaat
    15481 gtttgatttg cccatgcaaa atatctccaa attatattga agccttcttt gtcgcgcaaa
    15541 ctatatagca aaattcctgc tgtaaccaaa aacagaggta tacttatcaa caaacggttt
    15601 ttaataggtt tctggttaaa attaaacata tcagaaatca tcaaacgagc tgatctcaat
    15661 gctgtatcac cagaggtaat aggtgctgca ataactccta atatagctag caaaccacca
    15721 aaagtgccta gccaatcttt tgtaatgtca tttacaattt ctgcagcgtt tgatattccc
    15781 ataccatttt tttgaaaaaa ataagtggct gctgccgccc atattaaagc aacaatacct
    15841 tctgcaatca tagctccata aaagattctt cgtccttgtt tttcatttgt catacaacgt
    15901 gccatcaacg gagattgtgt tgcatgaaat cctgatattg cgccacaagc tatactgata
    15961 aacatcattg gaaatattgg catactctct ccgcccggat gtgtgtttgc caaaccatct
    16021 gttatttcag gcaatgcagg aaatttaatg tatatcatca ctagtattcc aactgccata
    16081 aatatcaaag cgaatgcaaa taatggatat atcttaccta ttattttatc aatcggtagc
    16141 atagttgcta taaaatagta caagaaaatg ataagaatcc aaaatgttgt attaaaaaat
    16201 gatggagtca aactgcctag taattcggca ggtcctgcaa caaatactgc accgaccaat
    16261 atcattagca aaacagcaaa gaatcgggaa aattgtttca tttgattacc taggtatttc
    16321 ccgtgtattt caggtaaatt gcatccgtca ttgcgtatcg atatcatacc tgccaaataa
    16381 tcatgcacag ctccaccaaa tatagtaccc aagactatcc atagatatga agacatgccg
    16441 aacttagcac ccataattgc accaaaaaca ggtcccaagc ctgcgatatt caaaaattgt
    16501 atcatgaaaa tacgccagcc agacatgggc acataatcaa ctccatctgt ctttaatatt
    16561 gccggtgtag ttttagaagc atcaggagca aataatcgct caataaaagc accatatgca
    16621 aaatatccta caacaagcag taataatgca aaactaaatg taatcatact tttatggaat
    16681 tttaaaccgc aaagttaatg atttagctct taaaagtaac aattacaagc aaatatgtac
    16741 taaatcacgc aaaaaaacaa gtacaaaaaa acaaattaca aatttatcag gaatatgttt
    16801 tataaatatt catttgaatt tttactttaa acaaaaagcc cataaaaact ttaaaattca
    16861 aataaaagta gtacctttat gccaattttt aattttaaca ttcattttac ttaaaatttg
    16921 attctaatat ggatattcat tctccaatag tacttttagt ggttggtatg ggaacagtat
    16981 tcgtagccct actgattatc atatattcag gcgaaggtct cattcgtttg gtaaatcgtt
    17041 ttttacctga agacgaaaag cctactgttg ataacaaaca aaccgcatca gtaagcccta
    17101 aaacagctca agcaatatct gcagctgttc aaatcatcac caataataaa gggatggttg
    17161 aaaaaatcga aaaaatataa taaaaccaaa taactatgtc aaagaagaaa gaagtaaaat
    17221 tttgcctaat gtttcgcgac atgtggcagt cggccggaaa atatgttcca cgtgttgatc
    17281 aattattaaa agtagctcct tctattgtaa aaatgggatg tttcgcacgt gttgaaacca
    17341 atggcggcgg ttttgagcaa gtgaatttat tgttcggaga aaatccaaac aaagctgttc
    17401 gcgaatggac aaaacctttt aatgaagccg gtatacaaac acaattgcta gatcgcggtt
    17461 taaatggtct tcgtatgagc cctgtacctg ctgacgttcg taaattattt tacaaagtaa
    17521 aaaaggcgca aggtacagat attgcacgta cgttctgcgg attaaacgac actcgtaata
    17581 ttattccgtc aattggatac gcaaaagaag gtggtatgat ttcacaatgt agtttgtgta
    17641 ttacattttc tccaattcat acggtagaat attacaccaa tatggctttt gaattaattg
    17701 aagccggtgc tgacgaaatc tgcttaaaag atatggcagg tataggtcgt cctgtttctt
    17761 taggaaaaat cgtaaaaaat atcaaagaaa aatatcctaa cattcctatt caatatcata
    17821 gtcatgcagg tccgggattt agcatggcat caatccttga agtatgtaaa gcaggatgcg
    17881 atttcgttga tgttggtatg gaaccacttt catggggcac aggacatgct gatatattgg
    17941 ctgttcaggc tatgttgaaa gatgcaggat ttgatgttcc tgaaatcaat atggaagcat
    18001 atatggaaac acgttcgtta attcaagaaa tgatggatga tttcttagga tattatatcc
    18061 cttccaaaaa cagattaatg agctcattac ttattgctcc gggacttccc ggaggaatga
    18121 tgggtagttt aatgacagat ttggaaacca atcttgaatc tatcaataaa tacaaggcca
    18181 aaaatggtca agctgctatt acacaagatc aacttcttat taagttattt aatgaggtat
    18241 cttatgtatg gccaaaaatg ggctatcctt gcttagttac tccatttagt caatatgtaa
    18301 agaatatggc tttgatgaat gtaatgcaaa tggaaaaagg caaagaacgc tggtctatga
    18361 ttgctgacga tatttgggat atgatgttag ggaaagcagg ccaattaccg ggtaaattag
    18421 acaaagaatt aattgcaaaa gcaaaagaac aaggtctcga atttcataat gaagatcctc
    18481 aaagcaacta tcctgatgca ttggattcat tccgcaagga aatgaaagac aacaaatggg
    18541 aattaggtca ggatgatgaa gaattattcg aattagctat gcatccacaa caatatcgag
    18601 catacaaatc tggtcaagct aaagcagatt tcgaagccga cttagcaaaa cgcaaagctg
    18661 atcgtgaaaa ttgcaatgga tctttatcac taccacaaac tgtaacagtt gatgttaatg
    18721 gcgaaaaatt tgcggtaaca attggagcaa acaacggaaa agaaacttct gcaacaacta
    18781 cagtttccgc agcacccggc gaaggcacag ctttaacatc cccattggaa ggtaaatttt
    18841 acttagtaaa aaatgcatcg gacgccacag taaaagttgg tgattttgta aagaaaggcc
    18901 aaacaatatg ctacatcgaa gcaatgaaaa cgttcaatgc tattgcagct gaatttgacg
    18961 gagttatcac agaaattagt atgccaatag gtgcgacggt atcggaagat gatgtattga
    19021 tgaaaataaa agcataacac catggaagaa atattaaata aaatatatga aatgactgcg
    19081 atcagcaata tagtagctga tcctagattt cttatcatgt acgctttggc ttttattcta
    19141 ttatatctag gtataaagaa acaatatgag cctctgctat tggtacctat cgcttttggc
    19201 gtattgattg ccaattttcc tggaggcgat atgggtgtta cacaagctgc cgaagatggc
    19261 actattaagc ttattaacgg cgaaatagtg aacatatggg aaatgtcgtt accacaaata
    19321 gcacacgaac ttggtataat gaactttata tactatttgc tcattaaaac aggttttctg
    19381 cctcctatta ttttcatggg tgtaggtgca ttaaccgact tcggaccaat gcttagaaat
    19441 cttaaattag cagtttttgg tgcaggtgca caattcggta tattttcagt attacttgtt
    19501 gccagccaat tttttacaat gaatgaagct gcatctttag caattattgg tggagctgat
    19561 ggccctacag ctatttttac aacaataaaa ttagcgcctc acttactagg gcctattgca
    19621 attgcagcat actcttacat ggcattagta cctgtaatta ttcctttagt agttaaactt
    19681 acatgcacaa aaaaggaatt gagcattaat atgaaagaac aagataaact ttatccttct
    19741 aaaacagaaa taaagaatat gcgtgttctt aaaattttat tccctattgt tacaacaaca
    19801 attgttgcta ttatagttcc atctgcagta gccctagttg gtatgttgat gtttggtaat
    19861 cttgtaaaag agattggcgc caacacatca cgtttatttg atgctgcttc aaatagcatt
    19921 atgaatgctg caactatatt cttaggacta tctgttggtg caacaatgac agttgatgct
    19981 ttcttaaatt tcacaactat aggaattgta ataggtggtt tctttgcatt tggtttatca
    20041 atatttggag gtataatgtt ggtaaaaatc atgaatctct tttcaaagaa aaaaatcaat
    20101 ccacttattg gagcaaccgg tttgagcgct gttcctatgg catcacgtgt tgcaaatgaa
    20161 atagcattga agtacgatag tcgtaaccac gttttaaatt attgcatgtc ttcgaacgtt
    20221 gccggagtta tcggttctgc agttgcagcc ggtattctaa tttcgttctt aggataaaac
    20281 aactaattaa caacacacaa aaaggcagaa gaaaatcttc tgcctttttg tatctatata
    20341 atctaaaaaa attaggcttc tattcccgtg aaaagtttca acaagtatcc tatcaagaaa
    20401 cttacacctg caacactaaa acttagtatt gccatttcaa gaaatcgctt cttaaaactt
    20461 tcgcctctag ctacagaata ataaaaattg aatatcgcaa taataagcaa tgctgaaagt
    20521 aacattatta ccaacgaaat tataacatta gagcaaaaaa caaacggact tatcaatgca
    20581 ataaccgtta ttagatatgc aacaccagta tatacagatg cttttatagg atgtttatca
    20641 ctatcaccac cttccgattt tgttgaaagg tactcggaag atgccataga caaagctgct
    20701 gctattccgg taatacttcc ggttaaggca atgagtttat gatctcctaa ggccaatgta
    20761 aatccggcca aggctccggt aaactcaact aaagcatcat tcaaacctaa aacaacgctt
    20821 cccatataag acaaacgttc ttcatttatt aaagctatca aacgttgttc atgtacctgc
    20881 tcttctttag ctaaggcttt aatttcaggg atatcataca ttccatactt ttcacttgca
    20941 ccttgttcac ctgattccat taacttaatg gcaaatgtca aaccaaaagt acgcgcacaa
    21001 aatacatacc aataaacttt taacatatta ggctttggtg atgcacctga taatttttgc
    21061 aatattttgt aatgtttatt ctcttccgat gctatttgct ctaagactgc tttattcgct
    21121 gaatcctttt ctattgatgc taatttgcga tatataatgt actcggtaat ttcacttatc
    21181 tgagcaatta taatttgttt ttttactgat tcttccatga tttataaatc aacatacact
    21241 tacaccgctt cttactatat cactcggaga tacaactacc aatcttccat ccgaatcctc
    21301 agcactcaaa atcattcctt gactttctac tcctttaagt tttctgcttt caaagtttgc
    21361 tataaaacaa atttgttttc ctactaaaac ttcaggatta ggataatact tagctatgcc
    21421 ggatacaatt gttcttcctc ccatcccatc gtcaatctta aattgcaata gcttatcagc
    21481 ttttggtact ttaacacatt ctaaaattgt acctactctt atatcaagtt tgccgaaatc
    21541 atcaaaactg acttgttgtt taatttctgc aggtttaaaa ttattcagcg cattttgttg
    21601 tttaatattt aaaagacgct gtatctgagc ttcaattgca tcgtcttcta ttttctcaaa
    21661 taatagttca ggttgtgaaa cagattcgcc tgtcttcaat aaatcggtac gtcccaattc
    21721 attccaatta aacgaatcca tattaagaag attgcgcaat ttttgagagc tgaatggtaa
    21781 gaacggctca aatgcaattg ccaaattagc tgttatctgc aaagccaaat acataatagt
    21841 ttcgactcgc ttcatatctg ttttggctaa cttccaaggt tctgtatctg ctaaatattt
    21901 attcccaata cgtgccaagt tcatagcttc tttttgagca tctctaaact tgaacacatc
    21961 aagcaaatct tctacacttt tcttaacatc agcaaattct ttaagagttg cagaatcaca
    22021 ttcagttaat tcgccacaag caggtatttt cccatcaaaa tacttttgcg taaggaccaa
    22081 tgttctattt acaaagttcc cgtatatagc aactaattcg ttattgttgc gtgcttgaaa
    22141 atctttccat gtaaaatcgt tatcctttgt ttcaggtgca tttgccgtta acacataacg
    22201 taaaacatcc tgtttacccg gaaaatcaac cagatattca ttcaaccaaa ctgcccagtt
    22261 tctagatgtt gaaatcttgt cgccctctaa atttaaaaat tcatttgcag gaacattatc
    22321 aggcaaaata tatgaaccct ctgccttaag cattgttgga aatactatgc aatgaaatac
    22381 tatattgtcc ttgcctataa aatgaagcaa acgtgtatcc tcagatttcc accatttttc
    22441 ccaatcatta ggaagtaatt cttttgtgtt tgaaatatat cctattggag catcaaacca
    22501 aacatataaa actttcccct cggtattttc aatcggcaat ggaattcccc aatccaaatc
    22561 acgacttact gctcgcggct gcaatcccat gtctaaccaa cttttgcatt gtccgtaaac
    22621 attggttttc cattctttgt gtccatctaa gatccactcg cgcaaccaat tctcgtattt
    22681 atccaaaggc aaataccagt gcttagtttc cttcaataca ggcttactac cgcttattgc
    22741 agatttagga ttaatcaatt ctagtggtga taaagacgtt ccacatttct cacactgatc
    22801 accgtatgca tcttgattgt gacaatgagg gcattcgccg gtgatgtatc tatcagctaa
    22861 aaattgctta gcctcctcat cataatattg ctcagaagtt ttctcaacaa atccaccctt
    22921 atcatatatg gttttaaaga tttgcgatgc cacttcatga tgagttttac tagtagttct
    22981 agaatatata tcaaacgaaa taccgaattc ttcaaatgat tttttaatga tactatgata
    23041 acgatctact atatcttttg gccttaaacc ttctttcttt gcttttattg taataggtac
    23101 tccgtgttcg tcagaaccac ctataaaaag cacatcttct cctttaaggc gcaaataacg
    23161 aacataaata tcagcaggca cgtaaactcc tgccaagtga cctatatgaa ccggaccatt
    23221 tgcgtatggt aaagccgaag taacagtagt acgcttgaat ttcttttgca tgtttttcgt
    23281 tttaaattgc aaaaataatg ctttttactg aatttattgt acatttgtgg tatgtctaac
    23341 aattaaaacc acactgtaat gaaaaaagca ctgattttat tagtttgtac gtcattcttt
    23401 tcgtttgctg cttatgcaga taacgaagat atgattacta cagaccaact tccggttgtt
    23461 tctcaacagt ttattaagaa atacttccct aacgaaacag ttgcatacgc aaaatacgat
    23521 cccgaatttt tcgattctgc atacgaaatc acatttaaaa gcggtaatag tattgaattt
    23581 aacaaacaag gagaatggaa agattacgaa tgcaaaactt gcattgtacc ggacgaactt
    23641 cttcctaatg agatttcgtc atatataaaa gaaaaatacc ctgacaaaaa tatcgtaagt
    23701 atcgaaaggg gcaaaaaaac atatgaagta aaattatcaa acggacttga actaaaattt
    23761 aacctaagag gacaactttt agaaatcgat ttataataat ctattattag tgttctcttt
    23821 ataaacatgg aatatatact ttctcattct tggattcgcg gacaaaaatg ttttgttcgc
    23881 ggatattgtt ttgatgaaca atcaaatttt attaaatcgg aaaaattgtt gtctttattt
    23941 tcaggcatac aaaccgaaga agatttttca aaaaaaataa agcaattaaa tggaattttt
    24001 caagttataa tagagacatc acaaggggtt cttgccgcag gtgatattgc aggaacgctt
    24061 ccgttgtatt accgcaatta caataacaaa tgtttccttt ctgacaatgt atattcgcta
    24121 ttaaaaggca atgagacaat agataaaaat gcggaaaccg attatttaaa tcttaattat
    24181 actcttaata gcaaaactct cattaatggt atacacagaa ttgagccaat gacttatgca
    24241 agcattagca acaactcggt aaaaacatca tcatattatt cttatcgtat ctataaaaat
    24301 gagataaaca atattgatat acagtatcag ttttcggttg ccttgcttaa tatttttgga
    24361 agattaattg aatatattaa tggtagacag gtaattgttc cgttaagcgg aggttatgat
    24421 tctcgtctta tagttgctat gttatatcgg ctcaattaca aaaatattat ttgctatacc
    24481 ataggtgatg agaatagtga agaggtcgaa attgcaaaat cagttgctaa aagcttaggt
    24541 tttaagcatt attgcataga caatacatta atggcaaaaa aatgcgattt atccaaagat
    24601 gttgattttt tggactattg taagtatgtc ggacaactat gtaacaaaat atggacgttc
    24661 gatttttttt ctgttaaatg gcttaaagaa aatgggattg ctgaatatga tgctgttttt
    24721 gttccgggtc acagcggtga ttgcttggcc ggtagtcata gtagtaaaaa ccatattaca
    24781 cagcatagta cagcatcata tatggctgtt aaagtattag aagaccaaag ttttttagga
    24841 cgaaataaag aactgcaaaa taagattacc aagctattac aaaagaagga aaaaacactt
    24901 aggacatcta actttgacga ttttgtaatc aaaaatagac ttgcgaaata cattaatagt
    24961 gccactcgcg tatatagtta ttttggtttt gatatagctt tacctttttg ggatattgaa
    25021 atgatggact tttttaaaac attggatcca tcattaaagc agaaccaaaa attttatcat
    25081 aattatttgg attctagcct atttggacca ctaggcataa gttttaaagg cagactacaa
    25141 gcaagtgata tggatttaag acttcattgt attaagtcgt ttatcaaaag aatgctccct
    25201 ctaaaaatag cggtttcact atcacatacg cctgatttag tgggaatgaa cgatatatta
    25261 aaaccattca taaaagaact tgcgaaagtc ggcattaaaa caaaaactaa taacccaaat
    25321 gaaattatgg cactttggta cctataccaa gttaaacaga atttatgcgc gcattagtta
    25381 acataaagaa attagtatct cgcgaattta ttcgtcatac agcgactctt ataagcggga
    25441 atattattgc gcaagctata tcattgttaa tttatcccgt cttaacgcga ttatattcac
    25501 ctgatgactt tggtgttttt aacttattct taagtatagg aggttgtata gtgatactat
    25561 caacaggagc ctttcattat agcataatat tacctaagca agaaaaagat tcattaactt
    25621 gtgtgcgttt atgcgcatat tgcattatca ttgccacact aatttcttta ttaccggctt
    25681 tttttaaaac ttttaccgcc gatttattca acactactga cttagctgat tcgctaatct
    25741 atttgccttt gtttgttttg agtggaggtt tatggtcttg tataaattat tggtttataa
    25801 ggcaaaaatc atttaagcat atcgcagaat atcaaattac acttaacgcc tcaaactcag
    25861 gattaaaaac tgtttttggt ataattcaat cgggatacgg attaattatt ggtaccataa
    25921 taggacaaat aacggctatt tctatttcat taattcattg caagaaatac tttaagtcat
    25981 tttttaaata cggcaacttt agcgaattaa gacaaaccgc atcaaaatat gcagattttc
    26041 caagatatgc attacctcga aacctaataa attattttgg ttcgaactta cttgtcttgc
    26101 tgctaagccc gtatttcgga atggcaaaaa taggtatatt cggaatggct attgctctat
    26161 ctatgagacc aataatactt ataaacaatg ccctttcgca aactattact caaaaaataa
    26221 gttattgcat aaaagagcat acaagcgcag ttccgatgat taagaagtac tttaaatatt
    26281 ctataccaac tattattgta ggattcggag tcttatattt agtattacct gcaacatgtt
    26341 cgtggctact tggcgaaaca tggagagatg ccggcgaata tattcgtttg atgctaccat
    26401 ggttagcttg cttattgtgg ttttatcctt ttagttgtat tcctgatata ttaaacaaac
    26461 agcgtacaga tatgttgttt gagatcacaa ttacagtagt aagagtaatg gcaatcatta
    26521 tcggtattgc aataaatgat ttttatgtga ccatactgtt atacagctta ttatcatcac
    26581 taattattat aatcagaggt ttttggtttt ttaaattagc taaaacttgt acttagtttg
    26641 atttttaatg tattatcttt gaaaatgaat tcattagaat tattttaaac caatataaat
    26701 gaaacagaat agattaatat ggtcgcttat tgggagaata ttttaccccc actttaagtc
    26761 aaaatcatat ataaattcga ccttactgaa atttgtattt tggcaaaaaa tattaggtat
    26821 caatagaaat actccatggc ctgtacattt cacatcaaaa gttatatgct ctgaaaagat
    26881 tcaaaacgac aatggattta gagctccggg gtatgccatg ggatgttata ttgatggaag
    26941 gaatggtatc attatagaat caggatctat tgttggacct aaagtttcta taattagtca
    27001 gaatcacaac aaatcagact atagccggtt tgatgcaagt aatcctatta ttatacgcaa
    27061 aaattcatgg ttagcatgtg gttgcactat acttgcagga gtagaattag gagaacatac
    27121 cattgttgca gcaggtgctg tggttaccaa atcattttta gaaggtaatt gcattttagc
    27181 cggaaatcct gcaaaaaaga taaaagacat acctccatat aatgtttaat ttttcgtcaa
    27241 acataaatat gaataaccga gaactataca agcagctatg caatacacat cctgaaatcc
    27301 cattatttat gcaatattgg tggatggaag cagcttgtaa acctgattct tggaatgtgc
    27361 ttttctgtgg tgatgatgat aatacagaag cagtacttgt ttttcatata caagaaaagc
    27421 tagggataaa acgagttatt caaccaacct taactcagta taatggcatt tggattaatt
    27481 atcccaaaga tataagttta gataagcgtt acgatttcga aaacagagtc atggaaaatc
    27541 taattaatca gttagatgcc atgaatttag acttttacga gcagaaattt cattatagct
    27601 ttaccaactg gcaacctttt tattggaagc aatacaagca aaccacacat tacacatatt
    27661 taattgaaaa tattgcagat agagatgtag tatttgcaat gatgtcgcat aaaaaaagac
    27721 aaaaaaagat actttcatca gaagataatt ttaccctcga tactgacatg ccaataaacg
    27781 acttctacga gttttgcaaa aacacgatag aaaaaaaaca gcatgctcct attttgtaca
    27841 catttgatac tctgagaaaa atttatgatg catctgttga aagaaatcag gggatgattt
    27901 ttactatacg tgataaagaa aataacatac attgttcatt atttaccgtg tgggacgaca
    27961 attctgcata caatcttatt attgcaatta atccaaaata caaaagtagc ggagcctcat
    28021 catgcattat atggatggtt atggatgcac ttaaagacaa aaccttgaat tatgatttcg
    28081 aaggaagcat gattcaggga gttgccttaa agaatcagtc ttatggagca aaacaaattc
    28141 cttattctgt aatttcaaaa agcagctgta aatacaattt tataaaatcc ctaagaaatc
    28201 ttttacatat aggattttaa atatatctac tcaaaattac agtccacaca ttccgtttaa
    28261 gtcttattta gcttgttcta atcacatatt ttattgtaac tttgtgcatc ttaattttaa
    28321 ttgacgatga aacaatattt gttagacttg ctgagacttg tttaccctga aatatgttta
    28381 gtttgtggtg gatatttagt agaaggtgaa aaatgtatct gcttagagtg tgtacataaa
    28441 cttcctaaaa caggatacca tctgataaac gataatatgg cagaacaatt gctttgggga
    28501 agagtaaaaa ttgaatatgg aactgcttta tgtagatttc aaaaaggcaa ttctgtacaa
    28561 aaagtattgc atcaattaaa atataaaggc gaaaaagaat taggaattgt tcttgggcaa
    28621 ttttttggat acgaaatcca aggaagcaat atttctcagg ttgatgctat tattccggtt
    28681 ccactgcatc ccgtcaaact aaaaaaacga ggatacaatc agagtgaaca aatagctatt
    28741 ggcttatctg aagtattgaa tataccaata gtaaacgatc tgttatatag aaaattagca
    28801 aacagcacac aaacaaagaa aaacgtttac gaacgttggg aaaattctca aaacattttt
    28861 gacataaagg aaaatagttt atccctctgc gaaaaacaca ttttattagt tgatgacgtt
    28921 ataacaacag gagctacaat agaggcttgt accgaagttt tacaaaaaat aaaaggactg
    28981 cgcgtgagtt ttgcagcctt ggcaattgca taataaatca aacatgacga ctaattttct
    29041 tacacactta gacgaaatag aatcaaccaa tgaatggatg ttgcaatcat tgaaacagaa
    29101 caaaaatctc aatcacggag atatggttat ggctgatttt cagagcaatg gccgcggaca
    29161 aatgaacaac cattgggaat cggaacgagg aaagaatcta ctatgttcaa ttatattgtt
    29221 ccctaaacgt atgcaagcta atgctcaatt ccctatatct gaagctgtgg cactggctgt
    29281 cgcagattat ttagatataa gaagttcttt tattagcgta aaatggccta acgacattta
    29341 ctggaacgac aaaaagattg ccggcatatt aattgaaaat aatttatcag gcaattattt
    29401 gagtgattgc attgtaggca taggcttaaa tctaaaccaa tccgagttta agagtgatgc
    29461 acctaatccc atatctctaa ataccataac aaaaagattc tttgatccta aaacagcagc
    29521 taaacgcatt cggatacatc taatgtcaag attttggcaa gcagaaaata ctcctgatat
    29581 tttgcatgag gaatatttaa aaaaactata tagaagagat attgcggcta catacaaaga
    29641 tgcaaatgga acctttatcg gcgtaataac tgatgttgaa actaccgggc atattgttat
    29701 taaagacgaa tccggaaatt tgagaagata cgcatttaaa gaagtacaat tcgtacttta
    29761 aaataaatac tatatgtcaa aacacaataa gctgaattaa aattagttat agtaaacata
    29821 aaaagataaa caagtttatt atctttgcag ttaataaatt ggttgatatt atagatatgc
    29881 gaaaaaattc aattataatt acattaatac ttacattcac aataaatgtg cttgcagcgg
    29941 acgaaggttt ggtaataata aatcaggaca accgcttaga tcaattagta catcaaaata
    30001 ccggcatttc acaacttccg gctaataacg caataagtgt ttcgggatac agagtacaat
    30061 tatactcagg taataacccg caaacggcta aacaagaggc cttaaaatta gaaaaagaac
    30121 tcactgaatt ttatcctgag attaagtcgt atgtaactta caatgctcca ttttggaaag
    30181 ttagaatagg agattttacg agctactacg aagctttatt atttagtcgc agattaaaag
    30241 ttgatttttc cagatttgaa aatgaaattt atgtaatgaa agaagatgcg gtaaaaccta
    30301 tatattttga aaataacggc ttaaacctaa taccatgaaa aaaacatttt taatcattac
    30361 ttcagcatta tgcattctat ttgcatcatg ttctgttgta gaacctgacc cttcacttga
    30421 cgacaaagat ttagtagagg gatataaatt ctctcagact attagtatca actacggcga
    30481 agacggcgaa cctcaaatca cgaatccctt aagtggtcaa ggagttgaaa ttactaataa
    30541 tggtcagcac gttacaatac gctcaacaat gacagatcct gtatgttacg aacttaaagg
    30601 tacttgtact gaaggttctg taaaaatata tggagaagaa aaattcaagc ttagccttaa
    30661 tggtctagat atgagttcaa cagatagttg tgctatcaac atacaatcgt caaaacgctg
    30721 ctttttagta ttggaagacg gtacaactaa taaaattact gatggtgccg gatacccgac
    30781 agacaaagaa gaacttacta gtggaggcga agacaagaaa ggtgcttttt ttagcgaggg
    30841 tcaagtaata gtaagtggta atggtactct tgaactaaga ggtagaaaca aacatgcact
    30901 tgcaactgac gaatatcttc gtattagaag cggaaatata actattacaa atgcagcatc
    30961 agatggttta catgttaaag aatattattg gcaagaaggt ggagaaatta atgtaaacaa
    31021 tcagaatgat ggcttgcaat gctctgaagg ttatataaag attgattccg gaatactcaa
    31081 tattgtaaca gctgatgatg gtatacttac caattatgga gatgaaccga ctgacactga
    31141 agctattgat gcatctatca caataaatgg tggagatatc aatattattg ctatgtcagg
    31201 taaaggtatg tattcaaatg gtaacattta cctaaatgca ggtaaattaa ctattacgtg
    31261 tattaaaaag gaaaaagtaa tatctacgaa tccgacaaag actgcaagta ttatacttgt
    31321 tgatacggaa acgcattaca cccctgcaca agactaattg taactatgtt aatacgctta
    31381 tacgaagaaa atccgaattc taaggaaatt gaaagaatcg tgggtattct aaaagatggg
    31441 ggtattatta tatatccaac agactctttg tacgcatttg gttgcgatat tacgaatgca
    31501 catgcggtag aaaagatttg taaaatcaaa aatattgatc cgggtaaaat gcctctgtca
    31561 tttgtgtgca gtaatataag ccacattagc caatattcgc aattagacaa caatacattt
    31621 aaactattaa aagaatgctt acccggacct tttacattcc ttctaaatgg taacagcaat
    31681 ctgcctaaac tatttaaaaa gaagaaaacc gtaggtgttc gtattcctga caatggtatt
    31741 gcgctagcat tagtagaagc cctaggaaac cccattttat caacttcaat ttttattgat
    31801 gaagacgaac ctgaatatag taccaatccg gaattgattg aagaaaatta tgcaggacaa
    31861 gtcgattgcg taattgatgg cggtggaggt tctttagagc cttcaacaat aa
//
LOCUS       DAOPID010000002        29813 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_1504_length_29813_cov_4.647490, whole genome shotgun sequence.
ACCESSION   DAOPID010000002 DAOPID010000000
VERSION     DAOPID010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 29813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29813
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1504_length_29813_cov_4.647490"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1447
                     /locus_tag="PK641_00170"
     CDS             <1..1447
                     /locus_tag="PK641_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPG54803.1"
                     /translation="KKQSKKARTAVSGGMYGSFNTALSGSYFIKDWSIGASVSHNQST
                     GYINNTDYNYTNLYLTSEYASKKLGEFKFRIGGQIKAFGSNAFYSTTYPDQFERTRTL
                     LIGLDWSKRLGNFGIIINSNYRRHYDQFELFRDTTNAPSWYTNPNYHLTDVSGISAKG
                     VWYSGIGKSSIGINFKDEHIFSNVLGDSLAEAKPIPFIDNTVFNLGKNRYYIRYYAEQ
                     LFLIKNFSAAIGLSGFYHSQFGSHFDFGLNTSYSFTDASKLYLNVNSATRMPTFTDLY
                     YKSATQVSNPNLQAEKSITAEIGGSVGIKWFNASINGYYRYGKDIIDWIQFEENGKWY
                     SMNHTVIHATGGDIILGAKYGKYLKNLQITYTFNFLSKNTDQYVSKYALDYLKHKVTL
                     NANINIYKGFGINLQYSYQNRNGSYYLPNGSISQYPDSHMLDVRIYWEGMGVKIYAEA
                     TNILNQIYYDYGNIPQPGIWVKAGISYLIGK"
     gene            1513..2919
                     /gene="asnS"
                     /locus_tag="PK641_00175"
     CDS             1513..2919
                     /gene="asnS"
                     /locus_tag="PK641_00175"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HPG54804.1"
                     /translation="MGISGRIKVAQALKMTCYGEKINVRGWVRTRRGNKNVGFIALND
                     GSTINNIQIVADIEKFGDEFLKPITTGACIGVTGLLVQSMGQGQNVEIQAENIEIYGA
                     ADPETYPLQKKGHTLEFLRDIAYLRPRTNTFGAVFRIRHNMAMAIHTFFHERGFYYWH
                     TPLITASDCEGAGQMFQVTTLNLYDLKKDDNGSIDYSKDFFGKQASLTVSGQLEGELG
                     ALSLGEIYTFGPTFRAENSNTPRHLSEFWMIEPEMAFYEIEDNMRLAEDFIKYCVKWA
                     LDNCREDVEFLNNMYDKELISRLESVIQEDFVRLTYTEGIKILEEAVAKGRKFEFPIG
                     WGIDLQSEHERFLVEEHFKRPVILTDYPKEIKSFYMKQNDDGKTVRAMDVLFPKIGEI
                     IGGSEREADYEKLKKHAQAMHVPEKDIWWYMDTRRWGSAPHSGFGLGFERLLLFVTGM
                     SNIRDVIPFPRTPNNAEF"
     gene            complement(3087..3467)
                     /gene="crcB"
                     /locus_tag="PK641_00180"
     CDS             complement(3087..3467)
                     /gene="crcB"
                     /locus_tag="PK641_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HPG54805.1"
                     /translation="MMFKILFIIGTGSFLGGILRYLISRFIQNSIISAFPYGTFVVNI
                     LGCFLIGLFYGISERSNIMSAEWRMFLTVGFCGGFTTFSTFANENLALLRDGNIFNFA
                     IYAGLSVFIGILATYSGNLITKLF"
     gene            complement(3457..4512)
                     /locus_tag="PK641_00185"
     CDS             complement(3457..4512)
                     /locus_tag="PK641_00185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPG54806.1"
                     /translation="MPPQLIKTSLLAYSYKNGDDLVDYVSQNGGYAHIMPDLKKTGFL
                     SWKKNIDNFFSDNHFDILHCHIDGWQSAVVAYYAKKHGVKVCAVHAHRSLEENSQSGR
                     LEQFINRYCSVHCFDLHFACNKVATTCVYGRKFLKDVYYLKNGLDISKYTPALCNFER
                     ESYNELFDIKKGQIVIGHIGRFSPIKNHKFLLQLALSMMSKQISFKMLFIGDGNDINL
                     VKKQVLELGLSDNVEFLAYRNDIIKLLKYMDVLLLPSFAEALSNVSIEVQAAGTPAIL
                     SDTIKTDCDLGLNLLQFKPLVINVWQEAILSAIKTQKPSASEIKSHFIESGFDSESIA
                     RYYFSILDEAITKKDDV"
     gene            complement(4602..5810)
                     /locus_tag="PK641_00190"
     CDS             complement(4602..5810)
                     /locus_tag="PK641_00190"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPG54807.1"
                     /translation="MKRIIILFIECFILTLSINAQSVDELQKRKERAMKNLEMTSNLI
                     SETKETTAKTMTKVNLLSAEIKERKVVINSLNAEINGINQQLSRLRSEATAKQKALES
                     IKKEYAALMYHQYYKKGNFDKLMFVLSAGNFAESYRRYRYMQQYSEYCKKKAVEIEEA
                     KKELNAKLEEVEAVRLKRLDVLNNRKQENSKLQSEKNRHSVMVSKLKRKEKDLRKELK
                     KQQKVADQLNAQIERKIAEEARKSEKESIKQGKKVDDVLTKEEKLVSGNFEKNQGRLP
                     WPVEKGIVVGSFGIQPHPVLERVTTNNKGIYIQCPSGSNARSVFEGEVTQCFSIPGSN
                     NAVIIKHGLYRTVYANLTKVNVKVGDKVSLKQTIGKIYSDPDEDNKTVLYFQVWKDKS
                     INNPENWLAH"
     gene            complement(5818..6606)
                     /locus_tag="PK641_00195"
     CDS             complement(5818..6606)
                     /locus_tag="PK641_00195"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HPG54808.1"
                     /translation="MLNRSNIILPLFLVFCIFLASCKTQKIAKNKELLTEPTTILQEY
                     GNKIKTIQARVEYQLPGAKPISVKAMMRVCIDSAIVISIQPFLSIEMGRLHFTKDSII
                     LIDRYHKQYACGLYKDLSSNISMSYEMIQALLFNRVFDPSNKRYMGFVGSNDNMWQSW
                     KGSVDAFDIEFLFDQGRYLRRTVLNSNDKVSYLSVEYTDFTNFTEIDYPMTANYMLFS
                     PTYKSTLNLYVDKVSFNTKIDLISSIPQGYTKITIPKLIKGLIQ"
     gene            complement(6596..8326)
                     /locus_tag="PK641_00200"
     CDS             complement(6596..8326)
                     /locus_tag="PK641_00200"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG54809.1"
                     /translation="MRLKASIVLLVGLLCLLQPFSLMAKTQKGFDTLYTAEFDSLFLD
                     ALCAKSSGKIQESIDLYKLCNQLNPRSAIVCYELGILYLKLDSVKLGIDYINKSCDYD
                     PENYYYQTNLANIYSKKGMYNESLKKCEFIAKKFPQNDNVFYQLSMLYTQTGQYDKAL
                     SALDKLEKRVGINKDITFEKVRLYRTLNKNNKAIKEIENFIKESPFDESAWIFKGDLE
                     MSTNQMDEALASYMKSLEIAPGNGYAMASLCSYYDYIGDKEKTDEYLYNIFKATDVSL
                     SNKLAFLERVAKYYERSPDYVIRLEKIYLSMIESDPENAQVHLNYSEFLGYIGRDEEC
                     IESLRTSVYLDPSCSDCWLLLFKTAFDKEDSVMIDKVLKDAMEAVPNSPEFCYFAGVK
                     ALDEKNNADALKYIQKAKENLSAAKSEELQKSVNSLLAELLRAEGKKQEARECMLEAL
                     SKAPNDLMLMNNYAYYLALDNEELDKAAEYGAKLVEKEPLESTYLDTYAYILMKQAKY
                     DLAIFYIRQALTYDSKEAPNFEIIEHYGDILYFLGNIDQAIEEWSKAKKINPNSSILN
                     EKIKQKKYVE"
     gene            complement(8323..8760)
                     /gene="dut"
                     /locus_tag="PK641_00205"
     CDS             complement(8323..8760)
                     /gene="dut"
                     /locus_tag="PK641_00205"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HPG54810.1"
                     /translation="MVTVKIVNKSKHQLPAYATAFSAGMDLRANLSESVELKPLERKL
                     VPTGLYIELPVGYEAQIRPRSGLAIKKGITVLNTPGTIDADYRGEICVILVNLSAEPF
                     IVEDGERICQMVIAQHAQVEWNEVGSLSETERGAGGFGHTGKK"
     gene            complement(8820..10925)
                     /gene="rnr"
                     /locus_tag="PK641_00210"
     CDS             complement(8820..10925)
                     /gene="rnr"
                     /locus_tag="PK641_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008016060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HPG54811.1"
                     /translation="MNKKELQTALIEKLPSLGNKAFNYKQIASAAGITKAADKSNIPM
                     VLAEMVLDGLIKEVDRGKFKAVLHSNFLVGIIDRNSNGKTHLVPEDGGEQIFIADRNV
                     NHAMKGDKVRILLYARRRNRQIEGEVVEILKRARETFVGVLSICNNVAFVVVDNRILS
                     NDIYVPLDSLNGGKDGQKVIVKMIEWPEKAKNPIGKIIDVLGNAGENNTEMHAILAEY
                     GLPYSYPEEVAREAEKIDISISDAEIAKRIDCRNITTFTIDPKDAKDFDDAISYRKLA
                     NGNFEVGVHIADVTYYVSHNDIIDKEAYDRATSVYLVDRTVPMLPEKLCNLVCSLRPN
                     EDKLTYSVIFEMNDKAEVLHSKISRAIIRSDRRFTYEDAQKVIETGEGDLSEAILALD
                     KLAKQLREQRFKEGAIAFERYEVKFEIDAAGKPLDVYFKESKDANKLIEEFMLLANKT
                     VAEHIGKVKKGTNPKTFVYRIHDEPNQEKLANLSLFIKKFGYKFKSTGKRQEVSSSIN
                     KLLDSVEGKKEQNLIETVTIRSMAKAIYSTENIGHYGLAFDYYTHFTSPIRRYPDMMV
                     HRLLSAYDDGASSVNQSKYEEYCEHCSDREQVAASAERASIKYKQVEFMSDKRGQVFD
                     GVISGVTEWGIYVEIISNKCEGMVPMRDMDDDFYTYDEKNYCIIGRRTHRCYRLGDQV
                     SIMVSKCNLDKKQLDFVLV"
     gene            complement(10956..11756)
                     /locus_tag="PK641_00215"
     CDS             complement(10956..11756)
                     /locus_tag="PK641_00215"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HPG54812.1"
                     /translation="MGFFGGLIGISLGWTLGGPIGALIGFIVGTTITSGVKLISNKST
                     GGQAYSGANTTTQGDFIVSLMVLVATVMKADNRVTKAELDEVKHFLSRHFNEQDSLEA
                     LQLLKNILKQEYDYEPVCSQIGSRMNYSGKLELIHWLFKVSCADGTLNNSELSLITRM
                     AQLMSISSGDFISIKAIYLAYSNGSYNSQSKASKQNKMTATTACQILEVNINASEQEI
                     KKAYRRMAMKYHPDKVNTLGEQVRKSATEKFKAVNEAYETLKVLKGFK"
     gene            12028..12480
                     /locus_tag="PK641_00220"
     CDS             12028..12480
                     /locus_tag="PK641_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG54813.1"
                     /translation="MKNKTDRLNIIKEIVTENTIGSQEDLLKALIDRGFELTQATLSR
                     DLKRLKISRSLTDDGKYRYNLPVNSYYGPKQTVKLIGVNSIEFSGSLAIMKTRTGYAS
                     GIAAEIDQNAAHAILGTIAGDDTILIIPREGFSRTEITNILSNLIENN"
     gene            12509..13120
                     /locus_tag="PK641_00225"
     CDS             12509..13120
                     /locus_tag="PK641_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPG54814.1"
                     /translation="MTADESHIKYVDTILKTIEDAAKVRGTGIAKRNPEYVKQKMLEG
                     KAVIALYGDEFAGFSYIETWGNNRQYVTTSGLIVPEKFRGLGIARRIKHTTFSLARKR
                     WPDAKIFSLTSGAAVMKLNTALGYLPVTFADLTDDEDFWKGCQGCVNYDVLQRTGRKY
                     CICTGMLFDPKEKMCIESAKRELKKDEQSHVIAKILSHLKHNK"
     gene            13129..14352
                     /locus_tag="PK641_00230"
     CDS             13129..14352
                     /locus_tag="PK641_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007483715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HPG54815.1"
                     /translation="MMEKKKVVVAFSGGLDTSYTVMYLAKEKGYDVYAACANTGGFSP
                     EQLKENEQNAYKLGATKYITLDVTKEYYDKSLRYMIYGNILRNNCYPISVSSERIFQG
                     MAIARYAKEIGASAIAHGSTGAGNDQVRFDMTFLVMAPGVEIITLTRDKALSRQEEVD
                     YLNKNGFFADFTKLKYSYNVGIWGTSICGGEILDSKQGLPESAYLKQIEKTESEKLEL
                     GFDKGEICCVNGEKFEDKIAAIQKIESICAPYGIGRDIHVGDTIIGIKGRVGFEAAAP
                     MVIIGAHRFLEKYTLSKWQQYWKDQVANWYGMFLHESQYLEPVMRDIEAMLESSQRHV
                     NGTVILELQARTFTTVGVDSPDDLVKNKFGEYGEMQKGWTSDDAKGFIKVLSTPLRAY
                     YSNHKEEGEELEKSL"
     gene            14365..15327
                     /gene="argC"
                     /locus_tag="PK641_00235"
     CDS             14365..15327
                     /gene="argC"
                     /locus_tag="PK641_00235"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HPG54816.1"
                     /translation="MIKVGIVGGAGYTAGELLRILINHPKTEIIFVNSSSNAGNKITD
                     VHEGMFGETDLVFADTFDFDNIDVLFLCSAHGESKRFLENNEIPEQVKIIDLAQDFRD
                     ESEGFVYGLPEMNLERIKKATKLANPGCFATCLQLGLLPLAANGFLKSDVHITGITGS
                     TGAGVKPGATSHFSWRSNNLSIYKAFTHQHLLEVNRSIKNLQPSFESEINFVPVRGDF
                     ARGIFVSMYTECDLELAEARKIYGDYYHDAAFTFMMEANPDMKQVVNTNKAIVHIEKY
                     GNKLHIISTIDNLLKGASGQAVQNMNLMFGLEETCGLGLKSLAF"
     gene            15346..16464
                     /locus_tag="PK641_00240"
     CDS             15346..16464
                     /locus_tag="PK641_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPG54817.1"
                     /translation="MELFNVYPLFPIEIVKGSGCKVYDKEGQEYLDLYGGHAVISVGH
                     THPTYVKAISEQVAKLGFYSNSVINGLQKEFASRLGKICGYDEYFLFLINSGAEANEN
                     ALKLASFHNGKKKVLSFKHSFHGRTSAAVKVTDNPKYVAPVNEGFDVTFVELGNIEAV
                     RSELQKGTYSSIIIEGIRGIGGIQIPDDEFMRQLRNECTKNNVVMIIDEIQSGYGRSG
                     KFFAHQYTGIKPDIITVAKGICNGFPMAGLLINPMFDPIYGQLGTTFGGNHLACAGAI
                     AVLDIIEKENLIDNAAKVGDYLLTELKKLPIKEVRGRGLMIGIEFEQSIKEIRDKLLF
                     EQKVFTGVAGTNTIRLLPPLCLSMSEAEEFIKRLKNCI"
     gene            16507..17301
                     /gene="proC"
                     /locus_tag="PK641_00245"
     CDS             16507..17301
                     /gene="proC"
                     /locus_tag="PK641_00245"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HPG54818.1"
                     /translation="MKNIAIIGAGNMGGAIARSLSQGTFPAENISVSDLNENSLRDLQ
                     NFNNKINVTSSNIENVEDAEIVLLAVKPWLVPSIIENIKTAINYDKQIFISIAAGVTF
                     EELKSLLEEEGKIPALFRIIPNTAIDVLQSVSIISSFNASKDQEDKILNIFGELGKAF
                     LVPENQLNAFMSLSSCGIAYAMRYVRAAVEGGVEIGIYPNVAKQVVIQTLRGAIDLLE
                     TNDSHPEVEIDKVTTPGGLTIKGLNEMEANGFTNSIIKGLKASLIE"
     gene            17319..17867
                     /locus_tag="PK641_00250"
     CDS             17319..17867
                     /locus_tag="PK641_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HPG54819.1"
                     /translation="MYIMMKEIAERLRGLRDSLDISSDEMAEKTGIDKELYAAYETGN
                     ADIPMNYLCKVAQTYGIDTAVLISGDETHAQSYFVTRKGLGISVERSKAYKYRDLAGG
                     FKNAKATPFLVTVEPNNNPIHLNTHEGQEFNMVEKGRMLLQIDGKDIILNEGDSIYFD
                     ASRPHGMKALDNEKARFLAIII"
     gene            17873..19531
                     /locus_tag="PK641_00255"
     CDS             17873..19531
                     /locus_tag="PK641_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPG54820.1"
                     /translation="MLERFLEKTLFSSQEDFEKNFKIKAPKDFNFGYDVVDAWAEENP
                     NKRALCWTDSNGKHIDFTYSDLKKYTDQTASYFQTLGIEKGDMVMLILKRRYEFWFSI
                     IALHKLGAVAIPATHLLTDKDIVYRCKSADIKAIVCVGEKVVLDHVANAKPHSPSLQH
                     IISIGDEITEGFEDFYKGIESAPTFVRPEFVNHNNDISLLYFTSGTTGEPKMVIHDFL
                     YPLGHIVTASYWHNLNEDSLHLTVADTGWGKAVWGKLYGQMIAGATIFVYEHEKFTPS
                     DMLKMIQDYKITSFCAPPTIFRFMIREDLRKYDLSSLKWGTIAGEALNPSVYNEFFEL
                     TGIKLREGFGQTETTCTVLTTPWMEPKPGSMGVPNPGYNVDIIMSDGRTAEVGEQGEI
                     IIRADHNNKPEGLFLGYYRDEKLTKEAYSDGIYHTGDMAWKDEDGYFWFVGRNDDVIK
                     SSGYRIGPFEVESALVSHEAVVECAITAVPDPIRGQIVKATVVLTSAYKDRAGEDLIK
                     ELQDYVKSITAPYKYPRIIEFVEELPKTISGKIRRVEIRNRDKQ"
     gene            19534..20610
                     /gene="proB"
                     /locus_tag="PK641_00260"
     CDS             19534..20610
                     /gene="proB"
                     /locus_tag="PK641_00260"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HPG54821.1"
                     /translation="MYRYKRIAVKIGSNVLSKADGTLDLERMESLVTQVAQLHKKGVE
                     IILISSGAVASGRSELKLKKKLDPIAARQLFSAVGQAKLINHYYDLFAKHNIACGQVL
                     TTKESFGSRTHYLTQKNCISVMLENNVVPIVNENDTISVTELMFTDNDELSGLIASMM
                     NMEALIILSNIDGIFDGNPNDPKSKVIPIVDNGKDLSQYIQTGKSSFGRGGMLTKTNI
                     AQKVADEGITVIIANGKRENILPQLLENSNTISTRFTPNPKPVSGVKKWIAHSESFAK
                     GEIHINKKAADALIGNKATSLLFVGITEIKGDFEKNDIVKIITDDSQILGVGKAQYDS
                     TKARLLIGKKGQRPLIHYDYLYLD"
     gene            20801..21109
                     /locus_tag="PK641_00265"
     CDS             20801..21109
                     /locus_tag="PK641_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG54822.1"
                     /translation="MMNKKVRKFAVFLFLTACAGCTNSLQEPLSTDPTILSVETPKQV
                     NFKAPTFTELQNKYMDIALNHELSQSSDSKLRAANLLVAEMTTDEKMEMIDLLTEQKN
                     "
     gene            21221..21955
                     /locus_tag="PK641_00270"
     CDS             21221..21955
                     /locus_tag="PK641_00270"
                     /inference="COORDINATES: protein motif:HMM:NF013559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallopeptidase"
                     /protein_id="HPG54823.1"
                     /translation="MQKSDGLEPIESMMISYHEGDSVISKMQVLKGIEENKNTTTMRA
                     ARIMLAKWGKEIHYRTCNGLSPVTKTNLRQAMNEWEEAVNYKFRFKEIPNSGWNQFVW
                     GIGCYWHICIKTCNNQYINGSATLGCVPWSLVQLKNDMQISTGLHEFGHVLGLIHEHQ
                     RPDRDKYITIHWNNILPQYKNQFYVMCNSYVYGDFDYQSIMMYQSYTGFSVIGPTHTK
                     NNGQLCFEGDIMLSVMDKQKIKEIYR"
     gene            21952..22506
                     /locus_tag="PK641_00275"
     CDS             21952..22506
                     /locus_tag="PK641_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG54824.1"
                     /translation="MNTKNNIICLTLMMFCMSCTTIDLDGYYARATYMQNTLSYAIDV
                     QCFYHPIKQNIANRCAEKNSSWSEPITIEPGETSLIMDYVEPDYINIYRHSDGLLLNN
                     EYGAMGKYIGSDSNKSVVFVLPIKDIYYDSKYAKSAIDWMLIPIGANCEGSNSVEYRK
                     NCYLSDGWAHKYVLALNPEAECFQ"
     gene            22568..23011
                     /locus_tag="PK641_00280"
     CDS             22568..23011
                     /locus_tag="PK641_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG54825.1"
                     /translation="MKSIKYLLSVAIAIICLSCNEQGGDPINVCCNSVATYAKNTLNY
                     SISIQCFYQPLNGEQLWCEQEPIAWSEPIEIQPGERMQVMDFVRPYRIKIFRTSDGKL
                     LSDAYGFAEPTVEHILLPQEELPNGWVLRLSEKMQKRLEFLCLIK"
     gene            22996..23232
                     /locus_tag="PK641_00285"
     CDS             22996..23232
                     /locus_tag="PK641_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG54826.1"
                     /translation="MSDKITNGCSYSGDYYIQDSKYLTENIDWDQYPLYIDRLSCGVE
                     NISDELRKKYYMWGVDGYAEVNCISLNPEAKCFQ"
     gene            23426..24670
                     /locus_tag="PK641_00290"
     CDS             23426..24670
                     /locus_tag="PK641_00290"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HPG54827.1"
                     /translation="MEQNWKEAALNASRKLNLINETVINNVLSKLADKIMENMNVLLS
                     ANQIDLNTMDKSNPMYDRLQLNEARLTDIASDIRKVASLPSPLNKTLEEKALANGLNL
                     RKMTVPFGVIGIIYEARPNVSFDVFSLCFKSGNVCVLKGGTDAYNSNCAIVKLIQSVL
                     TEYNLDINTIHLMPAGREATAELMDAVGYVDLIIPRGGSGLINFVRDNSRVPVIETGA
                     GVVHTYFDETGDIVKGAAIINNAKTRRVSVCNALDCLLINEKRLADLPALCEALAKSK
                     VVLEADNKAMTFLNSRYPIELLKKADENTYGTEFKDYRMGIKTVSNIDAAIAHISKYG
                     SKHSECIITQDVNVADKFQKEVDAACVYVNAPTSFTDGAQFGMGAEIGISTQKLHARG
                     PMALPELCSYKWLINGDGQIRK"
     gene            24682..26856
                     /locus_tag="PK641_00295"
     CDS             24682..26856
                     /locus_tag="PK641_00295"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPG54828.1"
                     /translation="MEQISNFLNLPFTDPVLIFSLILLIILFAPLIFDKIKIPHIVGL
                     IFAGALFGPNGLNVLAYDSSFHLFGQVGVLYIMFLAGIDVDMNDFKQNKNKSIVFGMY
                     TFIIPMVLGTITGIFFMYLIYNNLSGGEPITWVSGEASDSNTLIKYCALSAIVLASMY
                     ASNTLLAYPIVSRFGVSRNRSVNIAVGGTIITTVLALLVLAIVLEVARGEMNYIFWLR
                     FIISLSIFGFIIFYLFPRIARWFFKRYADNIVQYIFVLAMVFFASFLAKLAGIEYIIG
                     AFLAGITLNRLVPKQSPLMNRIDFVGNAIFIPFFLISVGMIVNFRILFDGYLTLLTAV
                     LMSVIATLSKYLSAKAARKTFKMSNSEESMIFGLTNAQAAATLAAVMIAYNFVIGYTA
                     SGEAVRLLSEEILNGTIIMILVTCIISSIVTERASKKIAISDISNEKSAGIYKPEHRI
                     LIPVSNPNTINSLMELAVLIRNKNEKQPIYALSVMSNNNYESQGAIESQKLLEKAVSL
                     GAATDNKVKMISRYDLNIASGISNVVKEKNISDVVMGLHSKTTFSDSFFGSMTDALLS
                     GVNRMICIFKSTQPMGTIKRVVVTVPADAEKESGFNKWCDRVFTLSEHIDAEIVFYCN
                     ANTTRSIASLAVARKNHIQPKFMPFENWDEFMTIKTAITSNDLLVVISARKLSPSYQT
                     SFEKLPTQLSESFQENSFIVLYPEQFKEGEVDQMQNSTTKII"
     gene            complement(26939..27520)
                     /locus_tag="PK641_00300"
     CDS             complement(26939..27520)
                     /locus_tag="PK641_00300"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPG54829.1"
                     /translation="MLLPYYKQGLIECGCDEAGRGCLAGSVYAAAVILPPDFSNELLN
                     DSKQLTEKQRYELRPIIESSALAWAVGVVIAEEIDEINILKSSIIAMHRAIDKLVIRP
                     EHLIIDGNKFTTYQPIPHTCVIKGDGKYMSIAAASILAKTYRDDYMRDLHKQYPVYNW
                     DVNKGYPTKKHRQAILNYGVSPYHRKTFRLLPE"
     gene            complement(27525..28499)
                     /locus_tag="PK641_00305"
     CDS             complement(27525..28499)
                     /locus_tag="PK641_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HPG54830.1"
                     /translation="MERKKNMKNTYYILLTAFLLVSCTENKANQTNQTTEKTASVPVF
                     NEDSCFNYVKVQTDFGARVPNTQSHRQCADYLSEKLRIHADTVIVQECLLKAYNGTML
                     QSKNIIASFNIATKNRIMLCAHWDSRPFCDEETEQTKQKKPVMGANDGASGVAVLLEL
                     ARIMDSVPSKIGVDIILFDAEDYGVSEVEGSYCLGSQYWARNLHETNYNPKYGILLDM
                     VGDKSAIFCKDAVSAHFAPDVQNKIWKRAAELGYSNLFVDKAVGALIDDHYYINLIAN
                     IPCIDIIHYDSEAGFPATWHTNNDIIDNISKSTLKAVGTVLTTIIYEE"
     gene            complement(28478..29542)
                     /locus_tag="PK641_00310"
     CDS             complement(28478..29542)
                     /locus_tag="PK641_00310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPG54831.1"
                     /translation="MIWILVFGFYAFLMIAYAYGWLKSQRKTDKVDVTECPKLTVVVS
                     LRNEESAVEHLMNQFENQSFKDFNLILVDDNSTDNTAALLQKYADGNKIIFVKANNQG
                     KKQAIRQAVSIIDEGLVVCTDADCSFGAFWLESIVNTYRQTNADLLIGPVMMDKNKPL
                     QALEFLSLSAVTGGSAFIKRPVMCNGANLAFVVDVYKSVMKHLHDEQASGDDMFLLSA
                     IKLYGGNIEYILDTNAIVTIEGKSQLNAFLNQRARWVSKSKAYTDREIMFVGATVVSA
                     QLLLILSLILIPWQRSLLLFWLVKYIFDLPLLFLASRFFKQESLLLWSIPASLLYPFY
                     VAASIIFAMIGKIEWKGRKI"
     gene            complement(29539..>29813)
                     /locus_tag="PK641_00315"
     CDS             complement(29539..>29813)
                     /locus_tag="PK641_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HPG54832.1"
                     /translation="LMIFLFLGLFARFSFLTNADSLSENILGMVAVFVGSSSWWFTLT
                     LIASIFHKKFNIRGLWFLNKITGSLIFIIALGGLILTLTGASIAGL"
BASE COUNT         9902 a         5254 c         5179 g         9478 t
ORIGIN      
        1 taaaaaacag agcaagaaag ccagaactgc tgtaagtgga ggtatgtacg gcagttttaa
       61 tacagcactt agcggcagct actttatcaa agattggtct attggggctt ctgtttcgca
      121 taatcagtct accggatata ttaataacac cgattataac tataccaact tgtatttaac
      181 aagcgaatat gcatcaaaga aactcggtga atttaaattc cgcattggtg gtcaaattaa
      241 ggcttttggg tcaaatgctt tttattctac tacttatccg gaccaattcg agcgcacaag
      301 aactttactg ataggtcttg attggagcaa gcgcttaggt aattttggta ttataataaa
      361 ctcaaattat agaaggcact acgaccaatt cgagctattt agggatacaa caaatgcccc
      421 atcttggtac acaaatccga actatcatct tactgatgta tcaggcattt ctgcaaaagg
      481 cgtttggtac tcaggcatag gtaaaagtag tataggaatt aattttaagg acgagcacat
      541 ttttagcaat gttttaggtg attccttggc agaagctaaa ccaattcctt ttattgacaa
      601 tactgttttt aatctaggaa aaaatagata ttatataaga tactacgccg aacaattatt
      661 tttgattaaa aatttctctg cagcaatagg tttatcagga ttttatcatt ctcaatttgg
      721 tagccatttt gattttggat taaatacttc gtattcattt actgatgctt caaagctgta
      781 tttaaatgtc aatagtgcta ctcgtatgcc tacatttacg gatttgtact ataaaagtgc
      841 tactcaagta agcaacccta atctgcaagc tgaaaaatca ataacagcag aaattggagg
      901 ctcggtaggt ataaaatggt ttaatgcctc aattaatgga tattacagat atggaaaaga
      961 cattattgat tggatacaat ttgaagaaaa tggtaaatgg tatagcatga atcacacagt
     1021 tatacatgca acaggtggtg acataatttt aggagccaaa tacggcaaat atttaaagaa
     1081 ccttcagatt acatatacat ttaatttttt gtcaaaaaat acagatcagt atgtatctaa
     1141 atatgctttg gattatctga aacataaggt aactttgaat gcaaatatca atatatataa
     1201 gggtttcggt attaatcttc aatatagcta ccaaaatagg aacggatcat attatttacc
     1261 gaatggatct attagtcaat atcctgactc gcatatgtta gatgtacgca tatattggga
     1321 aggcatgggt gtcaaaattt atgcggaagc aacaaatata ttaaaccaaa tatactatga
     1381 ttatggcaat attccacagc ctggtatatg ggtaaaagcc ggtataagtt atttgattgg
     1441 taaataatga ttatatgctg actaaataga gaaaaaattt agtacttttg cgctttcaaa
     1501 catttagaaa tcatgggaat atctggaaga atcaaagtag cacaggctct aaaaatgacc
     1561 tgttatggag aaaaaattaa tgtgagaggt tgggttagaa ctcgacgagg caacaaaaat
     1621 gtcggtttta tagcgttaaa tgatggatct acaattaata acatacaaat tgtagcagat
     1681 attgaaaaat ttggagacga atttttaaaa cctattacaa caggtgcttg cataggagtt
     1741 accggacttt tagtacaatc aatgggacaa ggtcaaaatg ttgaaattca agcagaaaat
     1801 attgaaatat atggtgctgc cgatcctgaa acatatccac tgcaaaagaa aggtcacact
     1861 cttgaatttt tacgcgacat agcttattta agacctagaa ctaacacttt tggagctgta
     1921 ttccgtattc gtcacaatat ggcaatggct atacacacat tcttccacga acgcggattt
     1981 tattattggc atacaccatt aattactgca tcagactgtg agggtgcagg acaaatgttt
     2041 caggtaacaa cattgaattt atacgattta aaaaaagacg ataatggatc aattgattac
     2101 tctaaagatt tcttcggaaa acaagcgagt ctaactgtat caggccaact agaaggagaa
     2161 cttggagctt tgtctttagg cgaaatttat acattcggac ctacatttag agctgaaaat
     2221 tctaatactc ccagacatct ttcagaattc tggatgattg aacctgaaat ggcattctac
     2281 gaaattgaag acaatatgcg attggcagaa gatttcatta aatactgcgt aaaatgggca
     2341 ttagacaatt gtagagaaga tgtagaattt ttaaacaaca tgtatgacaa agaacttatc
     2401 agtcgacttg aatctgttat tcaagaagac tttgtaagac ttacatacac tgagggaatt
     2461 aaaatattgg aagaagcggt tgctaaaggt cgtaaatttg agttccctat tggttggggt
     2521 atcgatttac aatctgaaca tgagcgtttc ttggttgagg aacattttaa acgtccggtt
     2581 atacttaccg actatcctaa agaaatcaaa tcattttaca tgaaacaaaa tgatgatgga
     2641 aaaacagtaa gagcaatgga tgtcttgttc ccgaaaatag gcgaaatcat cggaggatct
     2701 gaacgtgagg ccgattatga aaaacttaag aaacacgcac aagctatgca tgttcctgag
     2761 aaagatattt ggtggtatat ggatactcgt cgctggggtt ctgcacctca ctccggtttt
     2821 ggtctaggat ttgaaagact actattattt gtaacgggta tgtcaaatat aagagatgtg
     2881 attcctttcc cgagaacacc taataatgct gagttctaaa tccgaataaa atcagtaaat
     2941 gacaatgacc cccaaaagtt agacaaaaaa acttttgggg gttgtagtat acactaatac
     3001 ttcgtacata aggttatgtt taaacttgtc agtattgcta atatatattc ttagcaattt
     3061 tacttcacct ttaagttctt ccatagttaa aatagtttag ttattagatt ccctgaatag
     3121 gttgcaagaa tgccaataaa aacactcaat cccgcatata ttgcaaaatt aaagatattt
     3181 ccatctctaa gtaaagctaa attctcatta gcgaatgtcg aaaaagtagt aaagccccca
     3241 caaaaaccaa cagtaaggaa catacgccat tcagcactca taatattgct tcgctcggaa
     3301 ataccataaa atagaccaat aagaaaacag cctaaaatat ttacaacaaa cgttccgtat
     3361 gggaaagcag aaataataga attttgaata aagcgtgata taaggtagcg taaaattcca
     3421 cctaaaaaac taccggtacc aataatgaat agtattttaa acatcatctt tttttgttat
     3481 tgcctcatct aaaatagaaa aataatatct agcaatgctt tccgaatcaa agccagattc
     3541 aataaaatgg cttttaattt cgctcgcaga aggtttttga gtctttatgg cagaaagtat
     3601 agcttcttgc caaacattta ttacaagagg cttaaattgc aataaattca agcctaaatc
     3661 gcaatcagtc ttaatagtat cagataatat agccggagtt ccggctgctt gaacttctat
     3721 tgaaacatta gacaaagctt cggcaaaaga aggtaataac aaaacgtcca tgtatttcaa
     3781 tagttttatt atatcattcc tgtaagctaa aaactcaaca ttatcagata aacctaactc
     3841 caatacctgt tttttcacta aattgatatc attgccgtca cctataaaca acattttaaa
     3901 tgaaatttgc ttagacatca ttgataatgc caattgcaat agaaatttat gatttttgat
     3961 aggactaaac ctaccaatat gacctattac aatttgacct tttttgatat caaacaattc
     4021 attatacgac tccctttcaa aattacacag tgcaggtgtg tatttactaa tatctaatcc
     4081 gttttttaaa taatacacat ccttcaaaaa ttttcgacca taaacacatg tagtagctac
     4141 tttgttacac gcaaaatgta aatcaaaaca atgcacggaa caatatctgt taataaattg
     4201 ctctaatcta ccgctttgcg aattttcttc aaggcttcta tgtgcatgta cggcacaaac
     4261 cttaacgccg tgtttctttg cataataagc tactacggcc gattgccaac catctatatg
     4321 acagtgcaga atatcgaagt gattatcaga aaaaaaatta tctatgtttt ttttccatga
     4381 taaaaaacct gtctttttta aatcaggcat aatatgggca tatcccccat tttgactaac
     4441 gtaatcgact aaatcatcac catttttgta agaataggcg agtaaacttg tctttattaa
     4501 ttgcggaggc atggcatacg ttatattacg tatgcctgag gtgattcctt cgttgaaatc
     4561 acgaaaaaat cgtagtaagt gatatttagg aactattggc gtcaatgtgc caaccagttt
     4621 tccggattgt taatagattt atccttccac acttggaaat ataacacagt cttattatcc
     4681 tcatctggat ctgaatatat ttttcctatt gtctgtttca atgatacctt atcaccaact
     4741 tttacgttaa ccttagtaag attcgcatat actgttctat aaagaccatg tttaataata
     4801 acagcgttat tactacccgg tatagagaag cattgtgtta cctctccttc aaaaacagaa
     4861 cgcgcatttg agccactagg acattgaata tatatacctt tgttatttgt tgtaacacgc
     4921 tctaaaacag gatgcggttg tattccgaaa cttcccacta caatcccttt ttctacaggc
     4981 catggcaatc ttccttgatt cttttcaaaa ttgcccgaaa ctaatttttc ttcttttgtt
     5041 aatacatcat ctactttttt gccttgtttt atagattctt tttcagattt tcgagcttct
     5101 tctgcaatct ttctttctat ttgagcattt aattgatctg caactttttg ctgcttcttc
     5161 aattctttgc gtaggtcttt ctcttttcgt tttaatttag ataccattac agaatgccta
     5221 ttcttttctg actgcagttt tgagttttct tgcttgcgat tatttaaaac atctaaacgt
     5281 tttaagcgaa ctgcttcaac ttcttctaac ttagcattaa gttccttctt tgcctcttct
     5341 atttcaactg cctttttctt acaatactct gaatattgct gcatgtatct ataccttcta
     5401 tatgattctg caaaattgcc tgccgataaa acaaacatga gtttatcaaa attgcctttt
     5461 ttataatatt ggtgatacat taaggctgca tattcttttt tgatagattc caaagccttt
     5521 tgttttgccg ttgcctcact gcgtaaacgc gacaattgtt gattaatacc atttatttca
     5581 gcattcaaag aattaattac aacctttcgc tcttttattt cggcagacaa aagattaact
     5641 tttgtcatag ttttggcggt agtctctttt gtttctgaaa taagattact agtcatctct
     5701 aagttcttca ttgcacgctc cttacgtttt tgcaactcat ctacagattg tgcattaata
     5761 ctaagtgtta gtataaaaca ttcgatgaaa aggataatta ttcgtttcat ataaatatta
     5821 ttgtatcagg cccttaatta atttcggaat tgtaattttt gtatatcctt gaggtattga
     5881 acttataaga tcaattttag tattaaaaga aaccttatcc acataaagat ttagcgttga
     5941 tttatatgtc ggagaaaata acatataatt tgcggtcatg ggataatcta tttcagtaaa
     6001 gtttgtaaag tcagtatatt caaccgataa atatgaaacc ttatcatttg aatttaatac
     6061 cgttcttcgt aaatatcttc cttgatcaaa taaaaattca atatcaaaag catcaacact
     6121 tcctttccaa gactgccaca tattgtcatt actgcctaca aaacccatgt atcttttatt
     6181 tgaaggatca aacactctgt taaataataa agcttgaatc atttcgtaag acattgaaat
     6241 attagatgat aaatctttat ataatccgca agcatattgc ttgtgataac gatcaatcaa
     6301 aattatactg tcttttgtaa aatgtaggcg tcccatttct atgcttaaaa aaggttgtat
     6361 agagattact attgcggaat ctatacaaac acgcatcata gcttttactg atataggttt
     6421 ggctcccggc aattgatatt caacacgagc ctgaatcgtt tttatcttgt ttccatattc
     6481 ttgaagaata gtagttggct cggttaaaag ttccttattc ttagctatct tttgagtctt
     6541 gcaagacgcg agaaatatgc aaaaaactaa aaatagaggc aatataatat ttgatctatt
     6601 caacatattt tttttgtttt atcttctcat ttaaaatcga agagtttgga tttatttttt
     6661 tggccttact ccactcctca attgcctgat ctatatttcc tagaaaatat aaaatatctc
     6721 cataatgttc tattatttcg aaatttggag cttctttact gtcataagtt aatgcctggc
     6781 gtatataaaa aattgcaaga tcatatttgg cttgtttcat caatatatat gcataggtat
     6841 caagatatgt ggactctaat ggttctttct ctaccagttt tgctccatat tcagctgctt
     6901 tgtcaagttc ttcgttatct aaagccaaat aatatgcgta attattcata agcataagat
     6961 cattaggagc cttcgacaaa gcttcaagca tgcactccct tgcctcttgt tttttacctt
     7021 ctgctcgcaa aagttctgcc agtaaagaat ttacagattt ctgtaactct tctgatttgg
     7081 cagcacttag attttctttt gctttttgaa tgtattttag ggcatctgca ttatttttct
     7141 catccaatgc tttaacacct gcaaaataac aaaactcagg agaattaggc acagcttcca
     7201 tagcatcttt aagaacttta tctatcatca cactgtcttc tttatcaaaa gctgttttaa
     7261 aaagaagaag ccaacaatct gagcatgacg gatctaaata tactgaagta cgcaaagatt
     7321 ctatacattc ctcatccctt ccaatatagc ctaaaaattc tgagtaattc aaatgcactt
     7381 gagcattttc agggtctgat tctatcatac ttagataaat cttttcgagt ctgataacat
     7441 aatccggact cctttcgtaa tacttagcaa ctctttctaa gaatgctaat ttattagaaa
     7501 gacttacatc tgtagcctta aaaatattat ataaatattc gtctgttttt tctttgtctc
     7561 ctatataatc gtaatagctg cataatgaag ccattgcgta tccattaccc ggtgcaattt
     7621 cgagagattt catataactg gcgagagctt catccatttg attagtcgac atttccaaat
     7681 cgcccttaaa aatccaagca gattcatcaa aaggagattc tttgataaag ttttctattt
     7741 ccttaattgc cttattgttt ttatttaagg tcctatacaa cctgactttt tcaaatgtta
     7801 tatccttatt tatgcctact cgtttctcta atttgtccaa agctgataaa gctttatcgt
     7861 attgtcctgt ctgtgtataa agcattgaca attggtaaaa aacattatca ttttgtggaa
     7921 acttcttcgc gatgaactcg cactttttaa gagattcatt atacatccct tttttgctgt
     7981 aaatattagc caaatttgtt tggtaataat agttttcggg atcataatca catgatttat
     8041 ttatataatc aatccctagc ttcacagaat caagtttcaa atacaaaata cccaattcat
     8101 aacacacaat tgcactgcgt ggatttaatt ggttacaaag tttatacaaa tcaattgatt
     8161 cttgaatctt tccggaactt ttagcgcaca aagcatctaa aaacaggcta tcgaattcgg
     8221 ctgtatataa agtatcaaag cctttttgtg ttttggccat caaagaaaat ggctgcaaca
     8281 aacacaatag gccgaccaat aatactatac tagcttttaa tctcatttct tacctgtatg
     8341 tccaaatccg cctgcacctc tttctgtttc gcttaaggat cctacctcat tccactctac
     8401 ctgcgcatgt tgtgctatta ccatttggca gattctttcg ccatcttcaa caataaatgg
     8461 ctctgcagat aaattcacta gtatgacaca aatctctcct ctgtagtcag catcaatagt
     8521 accgggagta tttagaacag taattccttt tttaatagcc aatccacttc ttggtcttat
     8581 ttgcgcctca taaccaacag gcaattctat atataaaccg gttggtacta atttgcgttc
     8641 taatggtttt aattctactg attcagaaag atttgcacgt aaatccatgc ctgccgaaaa
     8701 tgctgttgca taagctggaa gttgatgttt cgacttgttt acaattttta ctgttaccat
     8761 aatttttagc tacaaaatta ctagtttaaa ttcatattac attacacaaa aacaaagaac
     8821 tatactagaa caaaatccaa ttgttttttg tctaaattac acttacttac cataatacta
     8881 acctgatcgc ccaaacgata acatctatgg gttctacgtc ctattatgca ataatttttc
     8941 tcatcgtatg tatagaaatc atcatccatg tcgcgcattg gtaccatgcc ttcacattta
     9001 ttacttatta tttctacata aattccccat tcggtaactc cggaaattac gccatcaaat
     9061 acttgacctc gcttgtctga cataaattct acttgcttat atttgattga tgctctttcg
     9121 gcagatgcag ctacttgctc acgatcggaa caatgttcgc agtattcttc gtacttcgat
     9181 tgatttacac ttgaagcacc atcatcataa gctgacaaca aacgatgaac catcatatcc
     9241 ggatacctac gaattggaga tgtaaaatgt gtataatagt cgaaagccaa tccgtaatgt
     9301 cctatatttt ctgtagaata tattgctttg gccatagaac gaatagttac tgtctctatc
     9361 aaattctgct ctttcttacc ttctacgcta tcaagcaatt tgtttattga ggaagacact
     9421 tcttgacgtt tcccggtaga tttaaattta tatccaaatt tcttgatgaa aagggataaa
     9481 ttagctaatt tttcttgatt aggttcatca tggattctat atacaaaggt ttttggattt
     9541 gttccttttt taactttccc gatatgctct gctactgttt tattagccaa taacataaat
     9601 tcctctatca atttatttgc atccttcgat tctttaaaat aaacatctaa tggtttaccg
     9661 gcggcgtcta tctcaaattt tacctcgtaa cgttcaaatg ctatagctcc ttctttaaaa
     9721 cgttgttcac gtaattgttt agctaattta tcaagagcta atatcgcttc gcttaaatca
     9781 ccttctcctg tttctattac tttttgagca tcttcgtatg taaatcttct atcggaacga
     9841 ataattgctc tggatatttt agaatgtaaa acttcggctt tatcattcat ctcaaaaata
     9901 accgaatagg ttaatttgtc ttcgttagga cgcaatgaac aaaccaaatt acatagtttt
     9961 tcgggaagca tcgggacggt tctatctacc aaataaacag atgttgctct atcgtatgct
    10021 tctttatcta ttatatcatt gtggctaacg tagtatgtta catctgctat atgcacgcca
    10081 acctcaaaat tgccattagc caatttgcga tacgaaatgg catcatcaaa gtcttttgca
    10141 tctttcggat ctatagtaaa tgttgtaata tttctgcaat caatacgctt agctatttct
    10201 gcgtcactaa ttgaaatgtc tattttttcg gcttctcgag ctacttcttc cggataagaa
    10261 tagggcaaac catactctgc caaaatcgca tgcatctctg tattgttttc gcctgcattg
    10321 cctaacacgt cgataatctt acctatcgga tttttagctt tttcaggcca ttcaatcatt
    10381 tttacaataa ccttttgacc atctttacct ccattcaaag aatccaatgg cacgtaaata
    10441 tcgtttgaca aaattctgtt atctactaca acaaaggcta cattattaca aatactaagt
    10501 acacctacaa atgtttctct agctcgttta agaatctcta caacctcgcc ttcaatttga
    10561 cgatttcttc ttcgcgcata caataatata cgaaccttgt cgcctttcat tgcatgatta
    10621 acatttctat ctgcgataaa tatttgctct ccgccatctt ccggaaccaa atgggttttt
    10681 ccattgctat ttctgtcgat gattccgacc aaaaagttac tatgaagcac tgccttaaac
    10741 tttccgcgat ctacttcttt tattaaacca tcaagcacca tttctgccaa aaccatcgga
    10801 atattagatt tatcggctgc ttttgtaatc cctgcggcag atgctatctg cttgtaatta
    10861 aacgccttat ttccaagaga aggtagcttc tctattaaag ctgtttgtaa ttcttttttg
    10921 ttcatatatt atattttata aactgtgtaa agacactact taaatccttt gaggacttta
    10981 agagtttcat aagcctcatt aaccgcttta aatttttcgg ttgcagattt acgcacttgc
    11041 tctcctaatg tatttacttt gtccggatga tacttcattg ccattcttcg ataagctttt
    11101 ttaatttctt gttcagatgc attaatattt acttctagaa tttggcaagc tgtcgtagct
    11161 gtcattttat tttgcttgct ggccttcgac tgcgaattgt atgagccatt tgagtatgct
    11221 aaatatatag ctttaataga aataaaatct cccgatgaaa tgctcatcaa ttgtgccatt
    11281 ctagttatga gagataattc tgaattgttt aatgttccat cagcacaaga aaccttaaag
    11341 agccaatgga ttaactccaa tttgcctgaa taattcatac gactgcctat ttgtgaacaa
    11401 accggctcat aatcatattc ttgttttaga atatttttca ataattgcag tgcttctaaa
    11461 ctatcttgct cattaaaatg tctcgacaaa aagtgcttta cctcatctaa ttcggctttg
    11521 gttacacgat tatctgcctt catcactgtg gcaaccaaaa ccattaagga tacaataaag
    11581 tctccttgtg tagttgtatt tgctccagaa taagcttgtc caccggtaga tttatttgat
    11641 attaatttaa ctcctgatgt tatcgtcgtg ccaacaataa aaccaattag tgctcctatc
    11701 ggacctccta gtgtccaacc caacgaaatt cctattaatc ccccaaaaaa ccccataata
    11761 tctttattct aaacgtacat acatcgtgta tgtacaatca agcaaagata gtttttttta
    11821 atagtaattc atatagctat aataaaaaaa gaaatcacat tttttacatg aaccccgaaa
    11881 attatatata taactcttga gatttacata aaataagcgt ccctatttat ttattttttt
    11941 cgaattttca tcgccatatg aataattatg tatatttttg caagaaattt gcaataatac
    12001 cgaatattta ttcaattatg cactgtgatg aaaaacaaaa cagaccgttt aaatattatc
    12061 aaagaaattg ttaccgaaaa tacaatcggt agccaagagg atttacttaa agccttaata
    12121 gatagaggtt ttgagttaac acaagcaaca ttatctagag atttaaaacg cctaaaaatt
    12181 tcaagatctc ttacagatga tggcaagtac agatataatt tacctgtcaa ttcatactac
    12241 ggaccaaaac aaactgttaa gctaatcggc gtaaactcta ttgaattttc aggatcacta
    12301 gcaataatga aaacaagaac cggatatgca agtggtattg ctgctgaaat agaccaaaat
    12361 gctgctcatg caatacttgg tacaattgca ggagacgata caattttaat aattcctcgc
    12421 gaaggattta gtagaacaga aataacgaac atactttcta atttgattga aaacaattaa
    12481 aaccgtggaa gctaaggata ttaaaataat gacggcagat gaaagccaca ttaagtatgt
    12541 tgacaccata ctaaaaacaa ttgaggatgc agcaaaagtt cgtgggaccg gaatagccaa
    12601 acgtaatcct gaatatgtca aacaaaaaat gcttgagggc aaggctgtaa ttgctcttta
    12661 tggcgatgaa ttcgcaggtt ttagctatat cgaaacttgg ggtaataata gacaatatgt
    12721 aactacatcc ggtttgatag tacctgaaaa atttagaggc ttgggaatcg caagacgcat
    12781 caaacatacc actttttcac ttgcaagaaa aagatggcct gatgcaaaaa tattcagtct
    12841 tacatccgga gctgctgtaa tgaaactaaa tacagccctt ggttatttac ctgttacatt
    12901 tgccgatctt actgacgacg aagacttttg gaaaggctgt caaggatgcg taaattacga
    12961 tgtgttacaa cgtacaggac gtaagtattg catttgtacc ggcatgctat ttgatcctaa
    13021 agagaaaatg tgcatagaat cagctaaacg cgaattaaag aaagacgaac aatcgcatgt
    13081 cattgcaaaa atattaagtc atttgaaaca taataaataa catatataat catggaaaag
    13141 aaaaaagtag tagttgcttt tagtggaggt ttggatactt catacaccgt tatgtacctt
    13201 gcaaaagaaa aaggctacga tgtatatgca gcttgtgcaa acacaggagg ttttagtcct
    13261 gaacagctaa aagaaaatga acaaaatgca tataaattag gtgctacaaa atatataact
    13321 ttagatgtta caaaggagta ttatgacaag agtttacgtt acatgatata tggaaatatt
    13381 ttgcgcaaca actgctatcc tatttcagta tcttcagaac gtatattcca aggaatggca
    13441 attgctcgtt atgcaaaaga gattggagca tctgcaatag cacatggctc gacaggagca
    13501 ggtaatgacc aagttcgttt tgatatgacc tttttggtaa tggcaccggg cgttgaaatt
    13561 ataacactta cacgcgataa agcattaagc cgtcaagagg aagttgatta tctaaacaaa
    13621 aacggatttt ttgcagattt taccaaactg aaatattctt acaacgtagg tatttgggga
    13681 acatcaattt gtggaggtga gattcttgat tcaaaacaag gtttaccgga atctgcatat
    13741 cttaagcaaa ttgaaaaaac cgaaagtgaa aaattagaac ttggtttcga taaaggtgaa
    13801 atttgctgtg taaatggaga aaagtttgaa gataagattg cagcaattca aaaaatagaa
    13861 tcaatatgtg caccatatgg cataggtcgt gacatacacg ttggtgacac aattataggt
    13921 attaaaggac gtgtcggatt tgaagcagct gctcctatgg taattatcgg agcgcaccgc
    13981 tttttagaga aatatacatt aagtaaatgg caacaatatt ggaaagatca agttgcaaat
    14041 tggtatggaa tgtttttaca cgaaagccaa tacttggaac ctgttatgcg cgatattgaa
    14101 gctatgctcg aaagttctca acgacacgta aacggtactg taattcttga attgcaagct
    14161 agaacattta caacagttgg agttgattcg cctgacgatt tagttaagaa taaatttggc
    14221 gagtacggtg aaatgcaaaa aggttggact tctgatgatg caaaaggttt tataaaagta
    14281 ttgtcaactc ctttacgtgc atattactct aaccacaaag aagagggaga ggaattagaa
    14341 aaatcattat aatatagaat caaaatgata aaagtaggga ttgtaggagg agcaggttat
    14401 acagcaggcg aattgcttcg catattgatt aatcatccta aaacagaaat tatttttgtt
    14461 aacagcagta gtaatgccgg taacaaaata actgacgtgc acgaaggtat gtttggtgaa
    14521 actgatttag tatttgccga tacatttgat tttgataata ttgatgtact atttttatgt
    14581 tctgcacatg gcgaaagcaa gagatttttg gagaataatg aaattccgga acaagtaaaa
    14641 ataattgatc ttgcacaaga ctttagagat gagtcagagg gatttgtata tggtctgcct
    14701 gaaatgaatt tagaacgcat taaaaaagct accaaacttg caaatccggg atgctttgca
    14761 acctgcctgc agttaggact acttcctctg gcagcaaacg gttttttaaa atctgatgtt
    14821 catattacag gtattacagg atcgacaggt gcaggtgtaa aacctggtgc cacaagtcat
    14881 tttagctggc ggagcaataa tttatcaata tacaaagcct tcacacacca acatttgtta
    14941 gaagttaatc gttctattaa aaatttgcaa ccaagcttcg aatctgaaat taattttgtg
    15001 cctgtaagag gtgattttgc tagaggaatt tttgtatcaa tgtatacaga atgtgattta
    15061 gaacttgcgg aagcgcgtaa aatatacggc gattattatc atgatgcagc attcacattt
    15121 atgatggagg caaatcctga tatgaaacag gttgtaaata caaataaggc aattgtacat
    15181 attgagaaat atggcaacaa actgcatatc atctcaacaa tcgacaattt acttaaagga
    15241 gcatcaggtc aggcagtaca aaatatgaat ttgatgttcg gattagaaga gacttgtggt
    15301 ttaggtctca aaagtttagc tttttaaatt ttaaaatgtg caattatgga attattcaat
    15361 gtatatccgc tttttccaat tgaaattgta aaaggttcag gatgtaaagt atacgataaa
    15421 gagggtcaag agtatttaga cttatatggt gggcacgcag taataagcgt aggacacacg
    15481 catcctactt atgtaaaagc cattagcgaa caagttgcta aattaggttt ttattcaaac
    15541 tcagtaatta acggattgca aaaagaattt gcatctcgtt tgggtaaaat ttgcggatac
    15601 gatgagtatt ttttgttttt aattaattcg ggagcagaag caaacgaaaa cgcactcaaa
    15661 cttgcatcat ttcataatgg caagaaaaaa gtattgtcgt ttaaacattc atttcacgga
    15721 cgtacatccg cagccgtaaa agttacggat aatcctaaat atgttgctcc ggtaaacgaa
    15781 ggttttgacg ttacttttgt ggagttaggt aatatagaag ctgttcgcag cgaacttcaa
    15841 aaaggcacgt actcatcgat aataattgag ggtatacgag gtattggagg aatacaaatt
    15901 cctgatgatg aatttatgcg tcaacttcgc aatgaatgta caaagaataa tgttgtaatg
    15961 attattgacg aaattcagtc aggatacgga cgtagcggaa aattctttgc acatcaatat
    16021 accggcataa aacccgatat tattactgta gctaaaggta tttgcaacgg attcccaatg
    16081 gcaggattat taataaatcc aatgtttgac cctatatatg gacaactagg tacaactttt
    16141 ggaggcaatc acttagcttg cgccggtgcc atagcagtac tagatattat tgaaaaggaa
    16201 aatctgatag ataatgcagc taaagttggt gattacctac ttaccgagtt gaaaaaatta
    16261 cctattaaag aagttcgtgg tagaggatta atgattggca ttgaatttga gcaatcaatt
    16321 aaagagatca gagataagtt attatttgag cagaaagtat ttacaggcgt tgcaggaact
    16381 aatacaatcc gtttgcttcc gccattatgc ttatcaatgt cagaggctga agaatttatc
    16441 aaacgcctta aaaactgtat ttaatatttt atacttacat ttgtagaaca actaaatata
    16501 agaaccatga agaacattgc aattatcgga gccggaaata tgggtggagc cattgcacgg
    16561 agcttatcac agggaacttt tcctgccgaa aacatatcgg ttagtgattt gaatgaaaac
    16621 tcgctacgcg atctgcaaaa ttttaataat aaaattaatg taacatcctc aaatattgag
    16681 aatgtagaag atgcagaaat cgttttgcta gcagtaaaac catggttggt tccatcaata
    16741 attgaaaata ttaaaactgc tattaattac gacaaacaaa tatttatttc aatagcagcc
    16801 ggagttactt ttgaggaatt aaaatctcta cttgaagaag aaggaaagat accggcacta
    16861 tttagaataa tacctaatac cgctattgat gtacttcaaa gtgtttctat tatttcatca
    16921 ttcaatgcgt cgaaagatca agaagataag attttgaaca tattcggcga attaggtaaa
    16981 gcatttttag ttccggagaa tcaacttaac gctttcatgt cattgtcaag ctgcggtatt
    17041 gcttacgcaa tgcgttatgt acgtgctgct gttgaaggcg gcgttgaaat aggcatttat
    17101 cctaatgttg ccaaacaagt tgtaatacaa actttacgtg gagcaattga cttgttagaa
    17161 actaatgatt ctcatcctga agttgaaatt gacaaagtaa caacaccggg aggcttaacc
    17221 ataaaaggct tgaatgaaat ggaagctaat ggcttcacaa attcgattat taagggatta
    17281 aaagcaagcc ttatcgaata agtattagtt attaaaacat atatattatg atgaaagaaa
    17341 tagccgaacg gctacggggt ttacgcgatt cattggatat ttcgtccgat gaaatggcag
    17401 aaaaaaccgg tatagacaaa gaattatatg ccgcttacga aacagggaat gcagatatcc
    17461 caatgaatta tctatgcaag gtagctcaaa catatggaat tgacactgct gtgcttattt
    17521 cgggtgatga aacgcatgct caatcatatt ttgtaactag aaagggtctt ggtatatctg
    17581 tagagcgaag caaagcctac aaatacagag atcttgcagg cggttttaaa aatgctaaag
    17641 ctactccatt tttagtaaca gttgagccta acaacaatcc tattcatcta aatacacacg
    17701 aaggacaaga attcaatatg gttgagaaag gtcgtatgct attacaaata gacggcaaag
    17761 atatcatttt gaatgaaggc gacagcatat attttgatgc aagcagacca cacggaatga
    17821 aagcactaga caatgaaaaa gcacgtttct tagcaattat catataatta ccatgctcga
    17881 aagattttta gaaaaaacat tgttctcttc tcaagaagat tttgagaaga actttaagat
    17941 aaaagcaccc aaagatttta atttcggtta tgatgtagtt gatgcatggg ccgaagaaaa
    18001 ccctaataaa cgcgctttat gctggactga cagtaatggt aaacatatag attttactta
    18061 ttcagacctt aaaaaataca cagatcaaac agcatcttat ttccaaactt taggaataga
    18121 aaaaggcgat atggtaatgc ttatacttaa acgccgctat gaattttggt tttctataat
    18181 tgcattacat aaattaggag cagtagcaat tcctgcaact catctattaa ctgataagga
    18241 tatcgtatat cgatgcaaat ctgctgatat taaggcaatt gtgtgcgtag gcgaaaaagt
    18301 agtattagac catgtcgcaa atgctaagcc acattctcca agtttgcagc atataatttc
    18361 tattggtgat gaaattaccg aaggattcga ggacttttac aaaggcattg aatcagctcc
    18421 aacttttgtt cgtcctgaat ttgtaaatca taataacgac atatcgctac tttatttcac
    18481 ttctggaaca acaggtgaac caaaaatggt tatacacgac tttttgtatc ctctaggaca
    18541 tatagttaca gcttcatatt ggcataactt aaacgaagac agcttacatc ttacagtagc
    18601 tgatacaggt tggggaaaag ctgtatgggg taaattgtat ggacaaatga tagcaggagc
    18661 aacaatcttt gtatacgaac acgaaaagtt cactccgtct gatatgctga aaatgattca
    18721 agattataag ataacttcat tctgtgctcc tccaaccatt tttcgtttta tgatacgcga
    18781 agatttacgc aaatacgacc tgtcttcatt aaaatgggga actatagcag gtgaggcttt
    18841 aaatccgagt gtgtacaacg aatttttcga attaacaggt ataaaattac gcgaaggatt
    18901 tggtcagaca gagactacat gtacagtact tactactcca tggatggaac caaaaccagg
    18961 ctcaatggga gttcctaacc ccggttataa tgttgatatt attatgtctg atggtagaac
    19021 tgccgaggtc ggagagcagg gagaaataat tattcgtgct gatcataata acaaaccaga
    19081 aggtcttttc ttaggatatt acagagatga aaaattaact aaagaagcat acagtgatgg
    19141 tatataccat acaggagata tggcttggaa agatgaagat ggctatttct ggtttgttgg
    19201 acgtaatgat gatgttatta aatcatccgg ataccgtatc ggacctttcg aggttgaaag
    19261 cgcattggta tcgcatgaag cagttgtaga atgcgctata acagcagtac ctgaccctat
    19321 tagaggacaa attgtaaaag caactgtagt actaaccagt gcatataaag atagggcagg
    19381 agaagatctt ataaaagaat tgcaggacta tgtaaaaagt attactgcac cttataagta
    19441 tccacgtata attgagttcg tagaagaact gcctaaaaca ataagtggga agattcgtcg
    19501 tgttgaaatt cgtaacagag ataaacaata atcatgtacc gctacaaacg catcgcagta
    19561 aaaataggaa gcaacgtgct aagcaaagct gatggtacgt tagatcttga gcgcatggaa
    19621 tctttagtaa ctcaagttgc tcaactgcat aaaaagggag ttgaaattat actgatttca
    19681 tcgggagctg ttgcatccgg acgtagcgaa ttaaaattaa aaaagaaatt agatcctatt
    19741 gcagccagac aattattttc ggcagtaggt caggcaaaac tcatcaatca ttactacgat
    19801 ttatttgcta aacacaacat tgcttgcggg caggttctta caacaaaaga aagttttggc
    19861 agcagaacac actacctcac acagaaaaat tgcatatctg tgatgctcga aaacaatgta
    19921 gtgcctatag taaacgaaaa cgatactatt tcagttaccg aacttatgtt tactgataat
    19981 gatgaacttt caggtctcat tgcctcaatg atgaatatgg aagctctaat aattcttagc
    20041 aatattgatg gcatttttga tggcaatccg aatgatccca aatcgaaggt aattcctatt
    20101 gtagataatg ggaaagactt atctcaatat attcaaaccg gaaaatcatc attcggaaga
    20161 ggtggaatgc ttaccaaaac caatattgca caaaaagttg ctgacgaagg catcaccgta
    20221 attattgcaa atggaaagcg cgaaaatatt ttgcctcaac tactagaaaa cagcaatact
    20281 atttctactc gctttactcc taatcctaaa cctgtttcag gcgtaaagaa atggattgca
    20341 cacagcgaaa gctttgcaaa aggagaaata cacataaata agaaggctgc tgatgcacta
    20401 ataggcaaca aagccacatc gcttttattt gtaggaatca ccgaaataaa gggtgatttt
    20461 gagaaaaatg acatcgttaa aattataaca gatgactctc agatcttagg agttggtaaa
    20521 gctcaatacg atagtacaaa agcacgctta ctcatcggca aaaaaggtca acgcccatta
    20581 attcattacg actacttgta tttggattga tagccttatt ctttaaaatt ggctgttgac
    20641 agagcagctt agtttcttta attcaagtat agacgtttcg cgcaacgtct atatttaatc
    20701 atcgtccgct acacattaaa cattattatg ccaaaaacgt ttgcaccatt aaaagatttt
    20761 attacttttg ctgccgaatt taataaatac aacacaaaac atgatgaaca aaaaagtaag
    20821 aaaatttgcg gtattccttt ttttaaccgc ctgtgccgga tgcacgaaca gcctccaaga
    20881 gcccctctca accgacccaa ctattttatc ggttgaaaca ccaaagcagg taaattttaa
    20941 agctcctaca tttaccgaat tgcaaaacaa atatatggat atcgcgctga atcacgaatt
    21001 atcgcaatcg tccgatagta aattacgggc agcaaacctg ttggttgctg aaatgactac
    21061 tgacgaaaaa atggagatga ttgatttatt gacagagcaa aaaaattaaa cgacaaccct
    21121 tcgcacagcg caggaaaaca gcgaagaaga tgctttttat aacgcgcaat atgacaccct
    21181 tgtacaagaa ttaaatagac atcccttggt catcgaagcg atgcaaaaaa gcgatggact
    21241 agagcctatc gaatctatga tgataagtta ccacgaaggt gacagcgtaa tatcaaaaat
    21301 gcaagtacta aaaggaattg aagagaacaa gaatacaaca acgatgcgag ctgcacgtat
    21361 tatgcttgca aaatggggaa aagaaattca ttatcgcact tgtaacggat tgtcgccagt
    21421 tacgaaaact aatttgcgcc aggctatgaa cgaatgggag gaagcggtaa attataaatt
    21481 tagatttaaa gaaattccca atagtggctg gaatcaattt gtatggggaa ttggatgcta
    21541 ctggcatatt tgtataaaaa cttgtaataa ccaatatata aatggaagtg ccacccttgg
    21601 ttgtgtacct tggtctcttg ttcaactaaa aaatgatatg caaatatcaa ccggattgca
    21661 tgaatttggg catgtattag gtctgatcca cgaacaccaa cgtcctgatc gcgataaata
    21721 tataacaatt cactggaata atattttacc tcagtataaa aatcaatttt atgtaatgtg
    21781 caatagttat gtgtatggcg attttgatta tcaatctatt atgatgtatc aatcctatac
    21841 tggcttttct gtaataggac cgactcacac aaaaaataat ggacaattat gctttgaagg
    21901 agatataatg ttatcagtca tggataaaca aaaaattaaa gagatatacc gatgaataca
    21961 aaaaataata ttatctgttt aacattgatg atgttttgta tgtcttgtac taccatcgat
    22021 ttagatggat attatgcaag agccacctat atgcaaaata ctttaagtta cgcaatagat
    22081 gttcaatgct tttaccatcc aataaaacaa aatatcgcga atcgttgtgc agaaaaaaac
    22141 agttcatggt ctgaacctat tacaattgaa cctggtgaaa caagtttgat aatggattat
    22201 gtagaacctg attacattaa tatttatcga cattcagatg gtttattgtt aaataatgaa
    22261 tacggtgcaa tggggaaata tatcggatcc gattcaaata aatctgttgt ttttgtactg
    22321 ccaataaaag atatttatta tgatagcaag tatgctaagt ctgctataga ttggatgtta
    22381 atacccattg gtgctaattg tgagggttca aactccgtag aatataggaa gaactgctac
    22441 ctaagtgacg gatgggctca taaatatgta ttggctttga atcccgaagc agaatgcttc
    22501 caataataca taaaccttgg ctatttttca ttagctgaac atattggtta ttttaatttc
    22561 aataattatg aaatcaataa aatatttgtt aagtgtggcg attgccatta tatgtttaag
    22621 ttgtaacgaa caaggaggag atccaattaa tgtgtgttgt aattcggtag ctacgtatgc
    22681 taaaaacacc ttaaactatt cgatttccat tcagtgcttt tatcagccgc tcaatggaga
    22741 acaactgtgg tgcgaacaag aacctattgc gtggtctgaa cctatagaaa tacagccggg
    22801 agaacgcatg caagtaatgg attttgtacg cccatatcgt ataaagattt ttcgcacttc
    22861 cgatggtaaa ctgcttagcg atgcatacgg atttgcagaa cctacagtag agcatattct
    22921 tctacctcaa gaagaactac ctaatgggtg ggttctacgt ctgtcggaaa agatgcaaaa
    22981 gagattggag ttcctttgtc tgataaaata acaaatggat gctcttactc tggtgattac
    23041 tacatacaag actcaaaata tttaacggag aacattgatt gggatcaata tcctttatat
    23101 attgatcgtc tttcatgtgg tgttgaaaac atatcggatg aattacgaaa aaaatattat
    23161 atgtgggggg tagatggtta tgctgaagta aattgtattt ctttgaatcc cgaagcaaaa
    23221 tgcttccaat agagaaatag tttgtaagta atatttatat aaaagggcgg agaataaatc
    23281 gttttccgcc cttagtatgt ttggatagcc ttagtcaatt atttaaaact tcttttaaga
    23341 aaattatttc acctttaacc cttcacttat gcacttaaat ccgccaaatt atcgtatttt
    23401 tgcaaaaatc gaaagactac gctttatgga acaaaattgg aaagaagcag cgctaaatgc
    23461 ttcacgaaag cttaatttga taaacgaaac tgttatcaat aatgtccttt ctaagcttgc
    23521 agataaaata atggaaaata tgaatgttct gttatctgca aatcagattg acttaaatac
    23581 aatggataaa agtaatccaa tgtacgacag actacagctt aatgaggcgc gtcttacaga
    23641 tattgcatcc gacatacgga aagtagcatc actaccatct cctcttaata agacattgga
    23701 agaaaaagcc cttgccaacg ggctgaactt acgtaaaatg actgttcctt tcggagttat
    23761 aggtataatt tatgaggccc gtccgaacgt aagttttgat gttttttctc tttgctttaa
    23821 aagtggaaat gtatgtgtgc taaaaggtgg cacagatgca tacaattcaa attgcgcaat
    23881 cgtaaaattg atacaatctg tcttaaccga atacaattta gacataaaca ccattcattt
    23941 gatgccggca ggacgtgaag ctactgccga attaatggat gcagtaggtt atgttgatct
    24001 aattattcca cgagggggca gcggtcttat taattttgta agagacaact caagagttcc
    24061 tgttatagaa acgggagccg gtgttgtgca tacttatttc gatgaaaccg gagatattgt
    24121 aaagggcgct gcaattatta ataatgcaaa gacacgtcgc gtaagtgtat gcaacgcttt
    24181 agattgtttg cttataaacg aaaagagatt ggcggatctt cctgctcttt gcgaagcact
    24241 tgcaaaaagc aaggtagttc ttgaggccga taataaagcc atgactttct taaacagtag
    24301 atatcctatt gaattactta aaaaagctga tgaaaatacc tatggaactg agtttaaaga
    24361 ctatcgcatg ggtattaaaa ctgtttcaaa tattgatgct gcaattgctc atatatcaaa
    24421 atatgggtca aaacacagtg aatgcataat cacacaagat gttaatgtgg cagataagtt
    24481 tcagaaagaa gttgacgccg catgcgtata tgtaaatgct ccaacaagtt ttacagatgg
    24541 tgcacaattc ggtatgggcg cagagattgg catcagcaca caaaagttac atgctcgcgg
    24601 acctatggct ttacctgaat tatgcagcta taaatggctt attaatggtg acggacaaat
    24661 ccgcaaataa caattaataa tatggaacaa ataagtaatt tcttgaatct tccatttaca
    24721 gatccggtat taatcttttc gttgatactg ttgattatct tgtttgctcc gctcatattt
    24781 gacaaaatca agataccaca tattgtgggc ttaatttttg cgggggcgct ttttggccct
    24841 aatggtttaa acgttttggc atatgatagt agtttccatt tatttggaca agttggtgta
    24901 ttatacatta tgtttttagc cggtattgat gtagatatga acgacttcaa acagaataaa
    24961 aacaaaagta ttgtgtttgg tatgtacact ttcatcattc caatggtgct tggtaccatc
    25021 acaggaatct tcttcatgta tttaatctac aataatttgt cgggaggaga gcctataaca
    25081 tgggtttcgg gagaagcttc agactcaaac actctaataa aatattgcgc actatctgca
    25141 atagttttag caagtatgta cgcatcaaac accttacttg cttaccctat tgttagcaga
    25201 ttcggtgtat cacgcaatag gtctgtaaat atagccgtgg gcgggactat tattactaca
    25261 gtgcttgcct tgttggtttt agctattgta ctggaagtag ctaggggaga aatgaattat
    25321 atattttggt tacgattcat catatctttg tcaattttcg gatttattat attttatcta
    25381 tttcctcgca tagcacgctg gttctttaaa agatatgccg acaatatagt gcaatatatt
    25441 tttgtactgg caatggtatt ttttgcatca ttcttggcta aacttgcagg tatcgaatat
    25501 attattggag cattcttagc aggtatcaca ttaaacagac ttgttcctaa acaatcgcca
    25561 cttatgaatc gaattgactt tgtaggtaat gcaattttca ttccattctt tctaattagt
    25621 gtcggtatga ttgttaattt ccgcatcttg tttgacggat atctgacttt acttacagca
    25681 gttcttatgt cggtaattgc tacactttcg aaatatttat cggccaaagc agcaagaaaa
    25741 acctttaaaa tgagtaattc agaagaatca atgatatttg gtcttacaaa tgcacaggca
    25801 gcagctacct tggcagcagt gatgattgca tacaattttg ttattggtta tactgcaagt
    25861 ggcgaagcag taagattatt gtcggaagag attcttaatg gaaccatcat tatgatattg
    25921 gttacttgta taattagttc tatagttaca gaacgagcat caaagaagat agcaataagc
    25981 gacatttcca atgaaaagtc tgcaggcatc tataagcctg aacatcgtat tcttataccg
    26041 gtatctaatc ctaacactat aaatagtttg atggaactag ctgttttaat tcgaaacaag
    26101 aatgaaaaac agcctattta tgcactaagt gtaatgagca ataataacta cgaatcacaa
    26161 ggtgctatag aaagtcaaaa attattagaa aaagctgtgt cactgggagc ggccacagac
    26221 aataaggtta aaatgatttc gagatacgat ttaaatattg caagcggtat ctctaatgta
    26281 gttaaggaaa agaatatatc tgacgttgtt atgggacttc acagtaaaac aacgttttct
    26341 gattcgttct tcggttcaat gaccgatgca ttactttcgg gtgttaaccg catgatttgc
    26401 atttttaaat caacacaacc aatgggtacg ataaaaagag ttgttgtgac tgtacctgct
    26461 gatgccgaaa aagaaagcgg atttaataag tggtgcgacc gtgtgtttac tctttccgaa
    26521 catattgatg ccgaaattgt attttattgt aatgccaaca caacgcgaag catagcctca
    26581 ttagcagttg cacgtaaaaa tcatattcaa cctaaattta tgcccttcga aaattgggat
    26641 gagtttatga ccataaagac tgctataaca agcaacgatt tgcttgttgt tatatctgct
    26701 cgaaaactga gtccatcata tcaaacatct ttcgagaaat taccaactca actctcagaa
    26761 agttttcaag aaaatagttt tattgtgctt tatcctgaac agtttaaaga aggtgaagtt
    26821 gaccagatgc aaaattcaac tacaaaaatt atttaggatt aaacggttcg catcgtaggg
    26881 gcgccaccta tgttttcgcc ctgtgttgtg tgtttgccca tacaaaatat cttagaattt
    26941 attcaggcaa caatctaaat gtttttcgat gataggggct gacgccataa tttaatattg
    27001 cttgtctgtg tttttttgta ggataccctt tgtttacgtc ccaattatat acaggatatt
    27061 gtttatgaag gtctcgcata taatcatcac gataggtttt agccaaaata gaagcggcgg
    27121 ctatcgacat atatttcccg tctcctttta ttacgcatgt atgtggtatt ggctgataag
    27181 tagtaaattt atttccatct attataagat gttccggtcg tataacaagc ttatctattg
    27241 cccgatgcat tgctattatt gaagatttaa gaatgtttat ttcgtctatt tcttcggcaa
    27301 ttacaactcc tacagcccaa gctaaagctg atgattcaat tattgggcgc aattcgtaac
    27361 gctgcttctc tgttaattgc tttgaatcat taagcaattc gttagaaaaa tcaggaggta
    27421 aaataactgc agctgcatac acagacccgg ctaaacatcc acggcctgct tcatcgcatc
    27481 cacattctat aagtccttgt ttgtaatacg gcaatagcat cttattattc ttcgtaaata
    27541 atagttgtca gcactgttcc aactgccttt aaagtagatt tcgatatatt atcaataata
    27601 tcattatttg tatgccaagt ggctggaaat ccggcctcag aatcataatg tattatatct
    27661 atacatggta tatttgcaat tagattaata taataatggt catcaattaa agctcctaca
    27721 gctttatcaa caaacaaatt actataaccc aattctgctg cgcgtttcca aattttgttc
    27781 tgaacatcag gagcaaaatg ggcagaaaca gcatctttac aaaatatcgc agacttgtct
    27841 ccaaccatgt ctaatagaat cccatacttc ggattgtaat tagtttcgtg caaatttcta
    27901 gcccaatatt gtgaacctaa acaataactt ccctccactt cagaaactcc ataatcttcg
    27961 gcatcaaaca aaattatatc aactcctatc ttagatggaa ctgaatccat tatacgagct
    28021 aactccaaca acacagcgac tccacttgca ccatcgttag ctcccattac aggttttttt
    28081 tgcttagttt gctcggtctc ttcgtcacaa aaaggtcttg aatcccaatg cgcacatagc
    28141 attattctgt ttttagtagc tatattaaaa gatgctatta tattcttaga ttgcagcata
    28201 gttccgttgt atgctttaag caaacattct tgcacaataa ctgtatcagc atgtattctc
    28261 aacttttctg acaaataatc agcacattgt ctatgagatt gagtgtttgg aactctggca
    28321 ccaaagtctg tttgtacttt tacataatta aaacagctat cttcattaaa aaccggtact
    28381 gaagcagtct tttctgttgt ctgatttgtc tgatttgcct tgttttcggt gcaggacact
    28441 aataaaaaag cagtaagtaa tatatagtat gtgtttttca tatttttctt cctttccatt
    28501 ctattttacc aatcatggca aagattatgc ttgctgctac gtaaaatgga tacaataaac
    28561 ttgcaggaat actccatagt aacaaacttt cttgtttaaa gaatcttgat gccaagaata
    28621 ataaaggtaa gtcaaatatg tacttcacaa gccaaaatag taacaaactt ctctgccagg
    28681 ggatcagtat taatgataag attaataata attgagctga tacaacagta gctcctacaa
    28741 acattatttc tctatcggta tatgcctttg attttgacac ccatctggct cgctgattca
    28801 aaaacgcatt gagctgactt ttcccttcaa tagttacaat agcgttagta tctagtatat
    28861 attctatatt accaccatat aattttattg cagacaacaa aaacatatca tctcctgatg
    28921 cttgttcgtc gtgcaaatgt ttcattactg atttataaac atcaactaca aaggctaaat
    28981 ttgctccgtt acacattacg ggtcgcttaa taaatgcact accaccggtt acagcagaca
    29041 aacttaaaaa ctccaaggcc tgcaatggtt tatttttatc catcattaca gggcctataa
    29101 gcaaatctgc attagtttgt cgatatgtgt ttactatgct ctctaaccaa aacgctccga
    29161 aagagcaatc tgcatctgta cacacaacca aaccctcatc tataatagat acggcttgac
    29221 gtattgcttg tttcttgccc tgattattag ccttaacgaa gataatcttg ttgccgtcag
    29281 catacttttg caataatgcg gcagtattgt cagtactatt atcatcaacc aatattagat
    29341 taaaatcttt aaaagattga ttttcaaact gattcattag atgttctaca gcagattctt
    29401 cattacgtaa agaaaccacc acagtaagct ttggacattc agtaacatcc actttatccg
    29461 tttttcgctg agattttaac caaccatacg cgtaagctat catcaaaaag gcataaaagc
    29521 caaaaactaa tatccaaatc ataggccagc tatagaggct cctgtaaggg taagtattaa
    29581 gcctccaagt gcaataataa atatcaagga accggttatt ttattcaaaa accacaaacc
    29641 acgtatgttg aattttttat gaaagatgct tgcgataaga gtaagtgtaa accaccatga
    29701 acttgaacct acaaaaaccg caaccatgcc taaaatattt tcagataaac tgtcagcatt
    29761 tgtcaagaag ctaaaacgcg caaataatcc cagaaataga aaaatcatta ggg
//
LOCUS       DAOPID010000003        23443 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_2265_length_23443_cov_4.306781, whole genome shotgun sequence.
ACCESSION   DAOPID010000003 DAOPID010000000
VERSION     DAOPID010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 23443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23443
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2265_length_23443_cov_4.306781"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PK641_00320"
     CDS             <1..218
                     /locus_tag="PK641_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG54833.1"
                     /translation="FGEMEVWALEAFGAAHVLQEILTIKSDDVMGRAKAYEAIVKGDP
                     LPQPGIPESLNVLLHELRGLGLSINLE"
     gene            231..4505
                     /gene="rpoC"
                     /locus_tag="PK641_00325"
     CDS             231..4505
                     /gene="rpoC"
                     /locus_tag="PK641_00325"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPG54834.1"
                     /translation="MAFRTDNKVKTNFKQIAIGLASPEEILEQSSGEVLKPETINYRT
                     YKPERDGLFCERIFGPTKDYECHCGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHIQ
                     LVVPVAHIWYFRSLPNKIGYLLGLPTKKLDAIIYYEKYVIIQAGIRTDLAEGDLLTEE
                     EYLDIVDSLPRENYLLEDTDPNKFIAKMGADAVYDMLTRLDLDSLSYELRDKASNDSS
                     QQRKTEALKRLQVIESFRASKNVNKPEWMIMKVIPVIPPELRPLVPLDGGRFATSDLN
                     DLYRRVIIRNNRLKKLIEIKAPEVILRNEKRMLQEAVDSLFDNTRKSTAVKADANRPL
                     KSLSDSLKGKQGRFRQNLLGKRVDYSARSVIVVGPELKMHECGLPKDMAAELYKPFVI
                     RKLIERGIVKTVKSAKKIVDRKDPVVWDILEYVMKGHPVLLNRAPTLHRLGIQAFQPK
                     MIEGKAIQLHPLACTAFNADFDGDQMAVHLPLGNDAVLEAQILMLASHNILNPANGAP
                     ITVPSQDMVLGLYYMTKPRPGALGEGLKFYSPKEVIIAYNERKVSLNAKITVKTKDVQ
                     NGLIITRLIDTTVGRVLVNECVPDEVGFINEVLSKKSLRDIISKVISVCGVARTADFL
                     DEIKNMGYYMAFKGCLSFNLQNVIIPKEKDELVQEGYEKVEAILNDYNMGFITYNERY
                     NQIIDTWTHVNSKLSNILMKQLSEDDQGFNSVFMMLDSGARGSKEQIRQLSGMRGLMA
                     KPQKAGAEGGQIIENPILANFKEGLSVLEYFISTHGARKGLADTALKTADAGYLTRRL
                     VDVSHDVIITEEDCGTLRGLVASELKNNEDVISSLYERILGRVSVHDIQHPLTGKIIV
                     GAGEEITEEAAKTIQNSPIEHVEIRSVLTCESKKGVCAKCYGRNLATGCMVQKGESVG
                     VIAAQSIGEPGTQLTLRTFHVGGVAANVAAENKITSRYDGILEIDELRTVTSDDNVTL
                     VVSRLAELRIIEPTTKVILTQQNIPYASTLFFKSGSTVKKGDVICEWDPFNAVIIAEG
                     SGKMAFESMIENITFKTESDEQTGLKERVIIESKDKNKIPSAHVLDEKGEVVRTYALP
                     VGAHISIEEKAKIKTGQILFKIPRAVSKAGDITGGLPRVTELFEARNPSNPAIVSEID
                     GEVTFGKIKRGQREIVITSKTGEERKYLVPLSKQILIQENDFIRAGGALSDGAITPSD
                     ILAIKGPTAVQEYIVNEVQDVYRLQGVKINDKHFEVIVRQMMRKVEIEDPGDTRFLEK
                     QVVDKQEFMAENDQIWGKKVITDVGDSTIFQRGQIVTARKLRDENSALKRKDLRPIET
                     RDAIPATSSQILQGITRAALQTTSFMSAASFQETTKVLNDAAINGKVDKLEGMKENVI
                     CGHLIPAGAGQAEFEKIIVASREEYEKIMEAKRNVIDVSEPANINE"
     gene            4644..6752
                     /locus_tag="PK641_00330"
     CDS             4644..6752
                     /locus_tag="PK641_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004347450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HPG54835.1"
                     /translation="MKRINALFLFLCLLLGINAQSLLSPDGNLKLIFQLDETGCPTYA
                     LQYKNIDVIKTSKMGFALKNEAALQNGFTILSSQNSSFDETWKPVWGEVNEIRDQHNE
                     LLVNLHQTRTNRYMQIRFRVFNDGIGFRYEFEKQDKLGYFKIQDELTNFNLTGDHKTF
                     WIPGNFDSNEYYYTTSNFSEINANKADGSGIGTHKLFSATGVQSPILMKTDKGLYILI
                     FEAGALDYPAMHLDANLKNLTLTSALAPDAYGTKAYMQTPCKTPWRTILVTDKATELL
                     ASKTILNLNEPCKIENTDFIHPQKYIGIWWEMHVPNKSSWNYADGYNIKIKDTDWNKL
                     KPNGRHGATTENTKRYIDFAAEHGFNGVLVEGWNIGWEDWAGNWKEEVFDFVTPYPDF
                     NVEELSQYAKSKGVKIIMHHETSGSVTNYERRIDDAIDFMKKYGYDAVKTGYVGWIIP
                     RGEEHDGQWMAQHYTRTIEKFANNRIMVDQHEPTRPTGQHRTYPNLMACEAARGNEYN
                     AWSSGNPPEHETIIPFTRLMGGPMDYTPGIFEIKMDYYKKGNLCQVHTTLAKQLALYV
                     TLYSPLQMAADLPENYEKHMDAFRFIKDVAVDWDDTKILEAEPGDYICIARKAKGSNN
                     WFVGSITDENARTSIIDLSFLDKDKTYDAVIYKDAKDADWQSNPMAYIIENKKVNNKT
                     TLKINLAKGGGCAISLRPRP"
     gene            complement(6735..8207)
                     /locus_tag="PK641_00335"
     CDS             complement(6735..8207)
                     /locus_tag="PK641_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HPG54836.1"
                     /translation="MKKRIAKIAQKNNNSYIYGIKLETLNIIVMYRVTKLFLYIVSLT
                     LLVSISACGGFGNDDDVVDLKQIKEIGKLRVITLFGPMSYFSYKDNDMGYEYEFASDL
                     CKEIGVEMELIIAPDEETMVEMLVNGEGDLIAYRLPSTIEYKDKVSFTNREYITNQVI
                     VQCKSDSMARDVLDLSGKVVHVKPNTIYADRLSDLNNEIGGGMEIVYAPDSVSTDDLI
                     AQTAMHKIPMTIADNDIARLNKTYFGNLNYDLDVSFPQRAAWAVSKNSPELLEYINNW
                     SKNSKKKLYFSQIYRKYFEKSKYFEAAGLTRIPGRNQISSFDDYFRKYASIPSWDWRL
                     LAAVAYKESKFDPQIVSWAGACGLMQLMPNTAISLGMTEDDFHVPELSIKAGAKYIKQ
                     LNNMFLSVQDPAERVKFVLASYNSGPGHIFDARALAEKYGKDPNVWHGNVETYIRLKS
                     ESTYYTDNVCRFGYCRGDDIANYVDVVLSKYEEYKLWARP"
     gene            8236..8553
                     /locus_tag="PK641_00340"
     CDS             8236..8553
                     /locus_tag="PK641_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG54837.1"
                     /translation="MRKIVTSLCLCLLLALGACSNSEPNSKVVGTYELSSTNMYGIEM
                     SIDQLIVTLEQMIPNTNFDCIREISLELKTDYTFETVSDCYFQFETPLTIGNGTYQMN
                     EKA"
     gene            8736..9212
                     /gene="ssb"
                     /locus_tag="PK641_00345"
     CDS             8736..9212
                     /gene="ssb"
                     /locus_tag="PK641_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPG54838.1"
                     /translation="MSVNKVILLGNVGKDPEVRYVDNNVAVANFTLATTERGYTTRDG
                     KTIPDRTEWHNIVVWRGLAETAEKYVKKGMQLYIEGKIQTRSWTDKDGNKRYTVDILG
                     DVMQMLGRKNDSNSIEVNDSNPAEASTTEKKVVSSKKAEEEPFSNVPQQDGDDLPF"
     gene            complement(9280..10089)
                     /gene="nagB"
                     /locus_tag="PK641_00350"
     CDS             complement(9280..10089)
                     /gene="nagB"
                     /locus_tag="PK641_00350"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007751131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HPG54839.1"
                     /translation="MRLIIEPDYANVSKWAANYVVAKINAFKPTDSKPFVLGLPTGSS
                     PLGMYRELIALNKSGKVSFKNVITFNMDEYVNLPKSHPESYYSFMWNNFFHHIDIKSE
                     NVNILNGNANDLVAECADYEVRIKKAGGIDLFLGGIGPDGHIAFNEPGSSLTSRTRIK
                     NLNTDTVIANARFFENDVNEVPKTALTVGVGTVMDAREVMILVSGHNKARALAAAVEG
                     GVSQMCTLSALQMHPKGIIVCDDAATDELKVGTYKYFKDIEKDNLNPTCLL"
     gene            10320..11408
                     /locus_tag="PK641_00355"
     CDS             10320..11408
                     /locus_tag="PK641_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HPG54840.1"
                     /translation="MQFKYIISQSKIKAYLIKCVQNNRIPHAQMFWGAAGIGKLSLAL
                     AYAQYLNCTNRTADDSCGKCPSCIQYQKAVHPDLHFVFPYTKIKGKEYCDDFLPEWRE
                     MLKNQYFSLQQWLEAIGADNKQGIIYSNESEEILRKLSLKSYESDFKTMIIWLPEKMH
                     ESCANKLLKILEEPPSNTVFLLVSEQPELLLSTIISRAQMIHIPNIQADDMKDLFPPE
                     IVRTANGSYLQAKMLLDQKEESQANFILYRMLMAYVFKKDLVSLKKFSEDMVAKGREP
                     IKSFLEYAQHVTRECFIANLQDNTLNYTYPYEAEFTSKFKRFVSIYNIDALTEQFSRA
                     SSDIERNVNAKIVFFDLGLQLMTKIKSS"
     gene            11413..12501
                     /gene="ricT"
                     /locus_tag="PK641_00360"
                     /pseudo
     CDS             11413..12501
                     /gene="ricT"
                     /locus_tag="PK641_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773373.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
     gene            12554..13042
                     /locus_tag="PK641_00365"
     CDS             12554..13042
                     /locus_tag="PK641_00365"
                     /inference="COORDINATES: protein motif:HMM:NF025474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HPG54841.1"
                     /translation="MMKNKILYIAGVSALILFGCVKNTAYNSYKGTDVNGWNQDTSVI
                     FDVDITDTVSAYNIILNVRHTTEYPYQNLWMFVRSCSPDGIVRDDTTACFLADNTGKW
                     LGQSYFSVYEMPVMFMDKIRFPKEGVYHFEIYQGMRDSMLKGIRDIGITIEKSDSKDG
                     KE"
     gene            13029..13793
                     /gene="trmB"
                     /locus_tag="PK641_00370"
     CDS             13029..13793
                     /gene="trmB"
                     /locus_tag="PK641_00370"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HPG54842.1"
                     /translation="MGKNKLQKFADMETFSHVFQYPFGKLQSGVVFPYKGKWNSFFGN
                     DNPIVLELGCGKGEYTVGLARKYPNKNFIGIDIKGARIWTGAKDSNETGMKNVAFLRT
                     NIELLNYFFASDEVSEIWITFPDPQMKKVRKRLTSTRFMQEYRAIMRKNGLLHLKTDS
                     PFLYQYTLLMAQKNNLYIEQCTDDLYHTPNIDENLLSIQTYYEQQWLERGMNIKYIRL
                     KLSSSAEWLEPDDIIEEDDYRSFNRSRRSANNQRTK"
     gene            13825..14880
                     /locus_tag="PK641_00375"
     CDS             13825..14880
                     /locus_tag="PK641_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HPG54843.1"
                     /translation="MTLYPKLILDALQHVRYPGTGEDIVTAGMVADNIRIEGNSVWLS
                     LMLKPNDPLQKSLVKASEQAIITYIGDFVNIKGHISVMQVEKETKTAEVPLSGVKCKI
                     AVSSGKGGVGKSTVSANLAIALQKQGYKVGLLDADIYGPSIPKMFGIENERPFANESG
                     MIVPIEKYGIKILSIGFFVNKESAVVWRGAMASNAIKQLLTEALWGDLDFFIIDLPPG
                     TSDIHLTIVQTIKLDGAIVVSTPQEVALADAVKGIDMFQNDKVGVPILGLIENMAWFT
                     PAELPENKYYIFGKDGVKKLAEERGIELLGQIPIVQSICESGDNGKPICLNNDGIVSS
                     AFDNVAKNSIFAQILQK"
     gene            14877..15233
                     /gene="rsfS"
                     /locus_tag="PK641_00380"
     CDS             14877..15233
                     /gene="rsfS"
                     /locus_tag="PK641_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005679873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HPG54844.1"
                     /translation="MMTTKNLVNTIVGGIQEQKGLRIVTADLTKIPNTICKYLIICEG
                     NSNTHVNSIALKTKDYVREHAKEKPIAMEGFENCEWIIADYGDIMLHIMQREPRAFYD
                     LEHLWADAKLTEIADL"
     gene            15235..17241
                     /gene="ftsH"
                     /locus_tag="PK641_00385"
     CDS             15235..17241
                     /gene="ftsH"
                     /locus_tag="PK641_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPG54845.1"
                     /translation="MENKQQPQKNPNNQPPKFRFNMYWLYGAIAVFLIAITFINEESP
                     KKDVPYSNFQEYVEKGYVEKIVVITNKNEADVSLKDDPQAIKIVLGEGGEKLYPKKPQ
                     LTVRIPSPEKLDDFITANSAKTGEKIDVRYEERKDYWDSLIWSFGPILLIVFIWVIAM
                     RRMGGGGAGGGIFSVGKSKAQLFDKGTAAIKVTFKDIAGLAEAKQEVQEIVDFLKNPA
                     KYTSLGGKIPKGALLVGPPGTGKTLLAKAVAGEANVPFFSMSGSDFVEMFVGVGASRV
                     RDLFRQAKEKAPCIIFIDEIDAVGRARGKNPSFNSNDERENTLNQLLTEMDGFGSNSG
                     VIILAATNRADVLDKALLRAGRFDRQISVDLPDIHERNEIFQVHLRPLKLAKDLNVDF
                     LAKQTPGFSGADIANVCNEAALIAARHNKTEVEKQDFLDAVDRIIGGLEKKNKITTAS
                     EKKSIATHEAGHASVSWLLEFANPLVKVTIVPRGKALGAAWYLPEERQLTTKDQMLDE
                     MCALLGGRAAEEVVFGKVSTGALNDLERATKMAYSMVVYYGMSDKLSNISYYDSTQSD
                     YSFTKPYSDKTSEIIDNEIKSIVNSQYQRALTIITENRSKHKALADLLVEKEVIFAED
                     VEHIFGKRPWASRMEEIIKESAKNDESKPIIEDEPQNTEDTSNE"
     gene            17234..18046
                     /locus_tag="PK641_00390"
     CDS             17234..18046
                     /locus_tag="PK641_00390"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPG54846.1"
                     /translation="MNNFWQRTLTGIAFVAVILTSIYFQDKYFIFTGIFGLVNILALY
                     EFYKITNISDRVNVPTIYLIICGTILYFGCFWLAKYGGEIVIVCYAMCIVALLIAELY
                     RKKAAPTHNIAFSLMGQAIVAVPFGLLNFIAIQQNVYWLYALFILIWTFDTGAYVFGV
                     SFGKHKIFPRISPKKSWEGEIGGAILTIIGSLIFAYFTPEVAIWKWIVFGSLIIIFGT
                     YGDLSESLLKRTASLKDSGTILPGHGGMLDRFDSLMLIIPVIYIYLQFIEEI"
     gene            18046..18717
                     /locus_tag="PK641_00395"
     CDS             18046..18717
                     /locus_tag="PK641_00395"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HPG54847.1"
                     /translation="MRIHKEGKNMLLVLLFTLIIVNGLLFYIFLTPTVPIIVAVLSVG
                     LFIFFTHFFRISNRILVADDTYVVAPADGKIVVIEPVMENDLLKEKRIQVSIFMSVFD
                     VHANWYPISGTVKHVSHQNGRKLAAYLPKSSSENERSSIIIESTNKQTILVRQIAGAL
                     ARRIVTYAKVGEKCTLNSQLGFIKFGSRVDMFLPLDAEILVDLHEETVGNETLIAKLK
                     HTSAE"
     gene            18778..20316
                     /locus_tag="PK641_00400"
     CDS             18778..20316
                     /locus_tag="PK641_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008781391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HPG54848.1"
                     /translation="MLVKSFGAAVQGIDATIITVEVNVSSGIKFFLVGLPDNAIKESH
                     ERIMSAFGHKGYKFPRQQVIINMAPADIRKAGSSYDLPIAMGILAASGELSEKLLNEY
                     VIMGELSLDGKVLPIKGALPIAVKAREVGFKGLILPKENAREAAVVNELDILGVDDIS
                     EVVDFLNEKTKIETTIVNTKEDFLNHVNKDEPDFADVKGQESVKRALEVAAAGGHNII
                     LIGPPGAGKSMMAKRIPSILPPLSLSEALETTKIHSVAGKLDKTTSLLHHRPFRSPHH
                     TISDTALVGGGTYPQPGEISLSHNGVLFLDELPEFKRSVLEVMRQPLEDRKITISRTS
                     CSIEYPASFMLVASMNPCPCGYYNHPERPCVCAPGAVQKYLSRISGPLLDRIDLQIEI
                     TPISFGQLSNRNQSESSAIIRERVINARNIQEKRYKNSNGVHCNAQMTVKLLHKYVQP
                     DEAGLKMLQYAMSKLHLSARAYDRILKVARTIADLDNSERVTAEHLSEAIGYRNLDRE
                     GWGG"
     gene            complement(20317..21129)
                     /locus_tag="PK641_00405"
     CDS             complement(20317..21129)
                     /locus_tag="PK641_00405"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HPG54849.1"
                     /translation="MLKRINETSSHLKTKINDIPKIGIILGTGLGNLATQITNRQEIN
                     YASIPNFPLSTVEGHSGKLIIGSLGAVRVLAMQGRFHYYEGYDMKEVTFPVRVMKSLG
                     IEILLVSNAAGGMNPDFKIGDLMIISDHINLFPENPLRGKNFNELGPRFPDMSAAYDQ
                     DLRTKAKKIAQQLGVQVKEGVYVGTSGPTFETPAEYRYFRRIGGDAVGMSTVPEVIVA
                     VHSGIRVFAISIITDLGIEGIVDTVSHEEVQRIADSVQPKMTAIMKGLIEEL"
     gene            complement(21035..22180)
                     /gene="lpxK"
                     /locus_tag="PK641_00410"
     CDS             complement(21035..22180)
                     /gene="lpxK"
                     /locus_tag="PK641_00410"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HPG54850.1"
                     /translation="MKSQFFKCLRIVCYPIGWIYGLVVMIRNYLFDAKIIKSTKYKIA
                     IISVGNLTVGGTGKTPHVEYLLHILSDKFNTAMLSRGYKRKSKGLVFANTINANASIL
                     GDEAYQVYSKFPQVKVVVDKDRREALREIESIYSNIDVVVMDDAFQHRYVKAGLSVLL
                     VDYNRIITEDKLLPYGDLREPASGKNRADIIVVTKCPIDLSPLEVRILRKKLYLRPFQ
                     SLFFSTYTYQKPVAVFKEISGELKEVGSVLLVTAIAQPKPLKEYLEEQYKIVQSVSYS
                     DHYLLSEKDWCDISERFRLLSGPNKYIFVTEKDAAKLVGCKYIPNDLKSYIYAIPLQI
                     KILQGKQDNFNNKFIDYVEKNKRNIVAPKDQNKRYSKDWHNIGYRFG"
     gene            22262..23281
                     /locus_tag="PK641_00415"
     CDS             22262..23281
                     /locus_tag="PK641_00415"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HPG54851.1"
                     /translation="MESINWSELSFGYIKTDCNIRCEYHNGAWGQLETHTDEYINMHM
                     AATCLHYAQEAFEGLKAFRGKDGKIRIFRIDENEKRLESSCNGIKMPCLPKGYFREAV
                     LSAVKKNARFVPPYESGASLYIRPVLFGYTPQIGVNPAKDYLFIVFVSPVGPYFKGGF
                     STNPYVITRNHDRSAPLGTGIYKVGGNYAASLAAHEEAHEKGYCSEFYLDAKEKKYID
                     ECGAANFFGIKNNTYITPKSTSILPSITNKSLMVLAEDLGLKVERRPILEEELDSFEE
                     AGACGTAAVISPIERLDDIDNNKSYVFSKDGKPGPICTILYNKLRAIQYGDEADIHNW
                     VTVLD"
     gene            23299..>23443
                     /locus_tag="PK641_00420"
     CDS             23299..>23443
                     /locus_tag="PK641_00420"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004342928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HPG54852.1"
                     /translation="MKLLILNGPNLNLLGTREPGIYGQEGFDTYLTKLNKRFPNVEIA
                     YFQS"
BASE COUNT         8139 a         4098 c         4314 g         6892 t
ORIGIN      
        1 gttttggtga aatggaagtt tgggcattgg aagcatttgg tgctgcgcac gtattacaag
       61 aaatcttgac tattaagtca gatgacgtta tgggacgtgc aaaagcttac gaagctattg
      121 ttaaaggtga tccactaccg caacctggta ttccggaatc actcaatgta ttattacatg
      181 agttacgtgg tttaggtctt agcatcaact tagagtaaaa agaaagcact atggcattta
      241 gaacagataa taaagtaaag acaaacttca agcaaatagc tattggattg gcttctccgg
      301 aagaaatcct agagcagtcg agtggcgagg tcctaaaacc ggaaaccata aactaccgca
      361 catacaaacc tgaacgtgat ggtctgtttt gcgaacgcat ttttggtcct acaaaggact
      421 atgaatgtca ttgcggaaaa tacaaacgta tccgttataa gggtatcgtt tgcgaccgct
      481 gcggtgtgga agtaacagaa aagaaagtac gtcgtgaaag aatgggacac atacaattag
      541 ttgttcctgt tgcacacatt tggtattttc gctcattacc aaacaaaatt ggttatcttt
      601 taggattgcc aactaaaaaa ttggatgcaa tcatatatta cgaaaaatat gtgattatac
      661 aagccggtat acgtaccgat ttagcagaag gagatttact aactgaagag gaatacctcg
      721 acatagttga ttctcttcct cgcgaaaatt atttgttgga agatacagat cctaataagt
      781 ttatcgcaaa aatgggcgca gatgccgttt acgatatgct tacacgtttg gatttggata
      841 gcttatcata tgaattacgt gataaagcaa gcaacgactc atcacaacaa cgtaaaacag
      901 aagcattaaa acgcttacaa gtgatcgaat cattcagagc ttcgaaaaac gttaacaagc
      961 ctgaatggat gattatgaaa gtaatccctg ttattccacc cgaattgaga cctttggttc
     1021 ctcttgatgg tggacgtttt gcaacttcag atttaaatga cttatacaga cgtgttatta
     1081 ttcgtaacaa tcgtcttaag aaacttatag agataaaagc tcctgaagtt attttgagaa
     1141 acgagaaacg tatgttgcag gaagctgtag attcattatt cgacaacacg cgtaagtcaa
     1201 ctgctgttaa agcagatgct aatcgtcctc ttaaatcttt atctgatagt ttgaaaggta
     1261 aacaaggtcg tttccgtcaa aacttattag gtaaacgtgt tgactattct gcacgttcag
     1321 taattgtcgt tggacctgaa ttaaaaatgc acgaatgcgg acttccaaaa gacatggctg
     1381 ctgaattata caaaccattt gtcattcgta agttaattga acgtggtatt gtaaaaacag
     1441 taaaatcagc taagaaaatt gttgatcgta aagatcctgt cgtttgggac atcttggaat
     1501 atgtcatgaa aggacatcct gtattactta accgtgctcc gactttgcac cgtttaggta
     1561 ttcaggcatt tcaacctaaa atgatagaag gaaaagctat tcagttacac ccattagcat
     1621 gtacagcatt caatgctgac ttcgatggtg accaaatggc tgttcactta cctcttggta
     1681 atgatgctgt tttggaagca caaatattga tgctggcatc acacaatatt ttaaatcctg
     1741 caaatggagc tcctatcaca gtaccttcac aagatatggt tcttggtctt tactatatga
     1801 caaaaccaag acccggtgca ctaggtgaag gattgaaatt ttattctcca aaagaggtaa
     1861 ttattgcata caacgaacgt aaagtatcat taaacgcaaa aataacagta aaaactaagg
     1921 acgttcaaaa cggacttatc attactagat taattgatac aacagttggt cgtgtattgg
     1981 ttaacgaatg tgtacctgat gaagtaggat ttatcaatga agtgttatct aaaaaatcac
     2041 ttcgcgatat tattagcaaa gtaatcagcg tatgtggagt tgctcgtaca gccgacttct
     2101 tggatgaaat caagaacatg ggttattata tggctttcaa aggatgtttg tcatttaact
     2161 tacaaaacgt catcattcct aaagaaaaag atgaattggt acaagaggga tatgaaaaag
     2221 ttgaagccat attgaatgac tataacatgg gtttcattac ttataacgaa cgttacaacc
     2281 aaatcattga tacttggaca cacgtcaact caaaattgtc aaatattttg atgaaacaat
     2341 tgtctgaaga tgatcaaggt ttcaactctg tattcatgat gttggattca ggtgctcgtg
     2401 gttctaaaga gcaaattcgt cagttgtccg gtatgcgtgg tttgatggca aaacctcaaa
     2461 aagccggtgc cgaaggtggt caaatcattg aaaaccctat tttggctaac tttaaagagg
     2521 gtctatctgt gttggaatac tttatttcaa ctcacggtgc tcgtaaaggt ttggctgata
     2581 ccgcattaaa gactgcagat gcaggttact taacacgtcg tttggtcgat gtatcacatg
     2641 atgttattat aactgaagaa gactgcggta ccttacgtgg attagtagct tctgaattaa
     2701 agaataatga agatgttatt tcttctcttt atgaacgtat cctaggtaga gtttctgtac
     2761 acgacattca acatccttta acaggaaaaa taattgttgg agccggtgag gaaatcacag
     2821 aagaagctgc aaaaacaata caaaattctc caatcgaaca cgtagaaata cgttcggtat
     2881 taacatgcga atctaaaaaa ggtgtttgcg caaaatgcta tggcagaaac ttggcaacag
     2941 gatgcatggt tcaaaaagga gaatctgttg gtgttatcgc tgcacaatct atcggtgaac
     3001 ccggaacaca gttgacattg cgtacattcc acgtcggtgg tgttgctgcc aacgtagctg
     3061 cagaaaataa gattacatct agatacgatg gtatattaga aattgacgag ttacgtacag
     3121 ttacttcaga cgacaatgtt acattagtag taagccgttt agctgaatta cgtatcattg
     3181 agccaaccac taaagttatt ttgacacaac aaaatattcc ttacgcatct acattgttct
     3241 tcaaatcagg atctactgtt aaaaaaggag acgtaatttg cgaatgggat cctttcaacg
     3301 ctgttatcat agctgaagga agtggtaaaa tggctttcga aagcatgatt gaaaatataa
     3361 cctttaaaac agagtctgac gaacagaccg gtttgaaaga aagggttatt attgaatcta
     3421 aggataagaa caagattcct tctgctcatg tattagatga aaagggagaa gttgttcgta
     3481 catacgcatt gccggttggt gctcacatct ctatcgaaga gaaagcaaaa atcaagaccg
     3541 gacaaatctt gttcaagatt ccacgtgctg taagcaaagc gggtgatatc accggtggtc
     3601 ttccgcgtgt aacagaatta tttgaagctc gtaacccttc aaatcctgca atagtatctg
     3661 aaattgatgg agaggtaaca ttcggtaaga tcaaaagagg tcaacgtgaa attgttataa
     3721 catcaaaaac aggagaagaa agaaagtatc ttgtacctct gtcaaaacag attctaattc
     3781 aagaaaacga ctttattcgt gccggtggag ctctttcaga tggagcaatc acaccttctg
     3841 atattttggc aatcaaagga cctactgcag tacaagagta catagtaaat gaagtccaag
     3901 acgtatatcg tctacaaggt gttaaaatta atgataaaca ctttgaagta attgttcgtc
     3961 aaatgatgcg taaagttgaa attgaagatc cgggtgatac acgtttcctt gaaaaacaag
     4021 ttgttgacaa acaagaattt atggctgaaa atgatcaaat atggggtaaa aaagttatca
     4081 ctgatgtagg tgattctact atattccaac gcggtcaaat tgtaacagca cgtaaattac
     4141 gtgacgagaa tagtgcttta aaacgtaaag acctacgtcc aatagaaact cgcgatgcaa
     4201 tacctgcaac atcaagtcag atcctgcaag gtattacaag agcagctcta caaacaacaa
     4261 gctttatgtc tgctgcatca ttccaagaga caacaaaagt tttgaatgat gctgcaatta
     4321 acggcaaggt tgataaactt gaaggaatga aggaaaatgt aatctgtggc catttaattc
     4381 cggcaggcgc tggtcaagct gaatttgaaa aaattatcgt agcatcacgc gaagagtacg
     4441 agaaaatcat ggaagctaaa cgtaacgtca ttgacgtttc agaaccggct aatataaatg
     4501 aataaaccta attaaggttt ctttaatacg aagaaaggct gcccatttgg cagcctttct
     4561 tgttttaaaa aaaaacaaaa ccaaacttct tacttcatat tgttttttac tatctttgga
     4621 cgataaccga aatcatatca aatatgaaac gaataaatgc tttatttcta ttcttatgcc
     4681 tattacttgg cattaatgca caatctcttt tatcacctga cggcaactta aaactaatat
     4741 ttcaactaga cgaaaccggt tgcccaacat atgcgcttca atataagaat attgatgtaa
     4801 ttaaaactag taaaatgggt tttgcactta aaaacgaggc agcattacaa aacggcttta
     4861 ctatattgtc atcacaaaac tcaagttttg acgaaacatg gaaacctgtt tggggtgaag
     4921 ttaatgaaat tagagatcaa cacaatgaat tactagttaa tttacatcag actagaacaa
     4981 accgttatat gcaaatccga tttagagtct ttaatgacgg aataggattc agatatgaat
     5041 ttgaaaaaca agataaacta ggatatttta aaatacaaga cgaattaact aatttcaact
     5101 taacaggaga tcataaaaca ttttggattc ccggaaattt tgattcaaac gagtattact
     5161 acacgacttc aaattttagc gaaataaatg ctaacaaggc tgatggatca ggtattggca
     5221 cacataaatt attttcagca acaggtgttc aaagtccaat actaatgaaa acagataaag
     5281 gattgtatat attgatattt gaagcaggcg cattagatta tcccgcgatg catttagatg
     5341 caaatctaaa gaacttgact cttacatctg ctcttgcccc tgatgcgtac ggcacaaaag
     5401 catacatgca aacgccatgt aaaacgccat ggagaaccat tttagttact gataaagcca
     5461 cagaactatt agcttctaaa acaatattaa acctaaatga accatgcaaa attgaaaata
     5521 ccgattttat acatccccag aaatatattg gtatatggtg ggaaatgcat gtccctaata
     5581 aatcatcatg gaattacgca gatggataca atataaaaat aaaggataca gattggaata
     5641 aacttaagcc taacggcaga catggcgcta ccacagaaaa tacaaaaagg tatattgact
     5701 ttgctgcaga acatggtttt aacggtgtac ttgttgaagg atggaatatt ggatgggaag
     5761 attgggccgg caactggaaa gaagaagtat ttgattttgt tacaccctat ccggatttta
     5821 atgtagaaga gttatcacaa tatgcaaaaa gtaaaggcgt taaaataata atgcaccatg
     5881 aaacatccgg atctgtgaca aattatgaac gcaggataga tgatgcaata gactttatga
     5941 aaaagtacgg atatgatgct gtgaaaactg gctatgtagg ttggattata ccacgtggag
     6001 aagaacacga tggccaatgg atggcacaac actatactcg tacaatcgaa aaattcgcaa
     6061 ataatagaat aatggtagat caacatgaac ctacccgccc tacaggtcaa catcgcacat
     6121 atccgaactt aatggcatgt gaggcagctc gtggaaatga atacaatgca tggagtagcg
     6181 gcaacccacc tgaacatgaa acaattatac cttttacaag acttatggga ggaccaatgg
     6241 actatacacc aggtatattt gagattaaaa tggattatta caaaaaaggg aatttgtgcc
     6301 aagttcatac tactttagca aaacaattag ctctttacgt tacattatat agcccattgc
     6361 aaatggctgc tgacctgcct gaaaactatg agaaacacat ggatgcattt cggtttataa
     6421 aagacgttgc tgttgactgg gacgatacaa aaatattgga ggcagagccc ggcgattata
     6481 tttgcatcgc tagaaaagca aagggttcaa ataattggtt tgttggatca ataaccgacg
     6541 aaaatgcacg cacatctata attgatcttt cattcttgga taaagataaa acttacgacg
     6601 cggtaatata caaagatgca aaagatgcag attggcaatc taatcctatg gcatacatta
     6661 ttgaaaacaa aaaggtgaat aacaaaacca cacttaagat taatttagca aaaggaggtg
     6721 gatgcgccat tagtctaagg cctcgcccat aatttgtatt cctcatattt tgaaagtacg
     6781 acatctacat agtttgcgat gtcatctcca cgacaatatc cgaatctgca tacattatca
     6841 gtataatatg tagattcgga ttttaatctt atatatgttt cgacatttcc gtgccaaaca
     6901 ttaggatctt taccatactt ttctgctaag gctcgcgcat caaaaatatg acccggacct
     6961 gaattatatg atgcaagtac aaactttaca cgttctgccg ggtcttgtac tgataaaaac
     7021 atattattca attgttttat atatttagca cctgctttaa tacttagttc aggaacatga
     7081 aagtcgtctt ctgtcattcc taaagatatt gcagtattag gcatcaactg cattaaccca
     7141 catgcaccgg cccaagacac aatttgagga tcaaatttcg attctttata tgctacggcc
     7201 gccagcaaac gccaatccca tgatggaata gatgcatatt tacgaaaata atcatcaaag
     7261 gatgatattt gatttctacc gggaattcgt gttagtccgg ccgcttcaaa atatttactt
     7321 ttttcaaaat acttgcggta tatttgtgag aaatacagtt tcttctttga attctttgac
     7381 caattattaa tgtattcaag taattcagga gaatttttag atacagccca tgcagctctt
     7441 tgcgggaaac ttacatctaa atcataattt aaattgccaa aataagtttt gtttaaacga
     7501 gcaatatcgt tgtctgctat tgtcataggt attttatgca tagctgtctg agctattaaa
     7561 tcatcagttg aaacagaatc cggagcatac actatctcca ttccaccacc tatttcatta
     7621 ttcaaatcag ataatctgtc tgcatatatt gtgtttggct ttacatgaac gactttaccg
     7681 gataaatcaa gtacgtcacg ggccattgaa tctgatttac attgcacaat aacctgattg
     7741 gtaatatatt ctcgatttgt aaaactaact ttatccttat actcaattgt acttggtaaa
     7801 cgatatgcaa tcaaatcgcc ttcgccattt accaacatct ctaccattgt ttcttcatca
     7861 ggtgcaatta ttaactccat ttctactcct atttctttac acaaatcact tgcaaattcg
     7921 tattcataac ccatatcatt gtctttataa ctaaaatacg acatgggtcc gaataatgtg
     7981 ataacacgta atttacctat ctctttaatt tgcttaagat ccacaacatc atcgtcgtta
     8041 ccaaatccac cacacgcact tatcgaaact aacaaagtca atgatactat gtataaaaat
     8101 aattttgtta cacgatacat aacaataatg tttaatgttt ctaattttat tccgtaaatg
     8161 tatgaattat tatttttttg tgctattttt gcaatacgtt ttttcatact agtactcata
     8221 taaatttatt gaatcatgag aaaaattgtt acttctttat gtttgtgtct tttattagca
     8281 cttggagcat gttctaattc agaacctaat agcaaagtag tcggcactta tgaattaagt
     8341 tcaacaaata tgtacggaat agaaatgtcg attgaccaat taatcgtcac tttggaacaa
     8401 atgattccca acacaaactt tgattgtata agagaaatct ccttggaact aaaaaccgat
     8461 tacacatttg aaacagtaag cgattgctac ttccaatttg aaaccccttt aacaattggc
     8521 aatggcacat atcaaatgaa cgaaaaagca taacttttac agagacagaa agcgaaacta
     8581 taattgaggg aacgatcgct gatgattaca atagtttcac aatagaaatg actgaacaat
     8641 ttggggatac tccaatgatt gttgtactta ctctaacaaa aaaataatct tattttttat
     8701 tcatatattt cttaatcttt aaaaaactaa tttttatgtc agtaaacaaa gtaatccttt
     8761 taggaaatgt gggcaaagac cctgaagttc gttacgttga taacaatgta gcagttgcaa
     8821 actttacatt agctacaaca gagcgtggtt atacaactag agatggaaaa accattcctg
     8881 accgcacaga atggcacaat attgttgttt ggcgcggatt ggcagaaact gctgaaaagt
     8941 atgtcaaaaa aggcatgcaa ttatacatag aagggaagat tcagactcgc tcgtggactg
     9001 ataaggatgg caacaaacgc tatacagtag acattttagg tgatgtcatg caaatgcttg
     9061 gacgcaaaaa tgattctaat tcaatagaag taaacgattc taatccggca gaagcatcta
     9121 caactgagaa aaaagtagta tcatctaaaa aagctgagga agaaccattt tcaaacgttc
     9181 ctcaacaaga tggagatgat ctaccatttt aatagaataa aacaatcaaa aaaagcgcat
     9241 ctttacagat gcgctttttt attgcattta tatcttactt tatagtaaac aagtaggatt
     9301 taaattatcc ttctcgatat ctttaaaata tttatatgta ccaactttaa gttcatctgt
     9361 agcagcatca tcacatacga taataccttt tggatgcatt tgtaaagcac ttaacgtaca
     9421 catttgacta acaccgcctt caacagctgc tgctaatgca cgcgctttat tatgtccact
     9481 tactaaaatc ataacctctc tcgcatccat cacagttcca acaccaactg tcaatgctgt
     9541 tttaggaact tcgttaacat cattctcaaa aaaacgagca tttgctatta cagtgtctgt
     9601 gttcaaattc tttattcgtg tgcgagatgt taatgatgat ccgggttcat taaatgcaat
     9661 atgaccatca ggtcctatcc cacctaaaaa cagatcgata ccccctgctt ttttaatacg
     9721 aacttcataa tcagcacact cagcaaccaa atcattagca ttcccgttta aaatattgac
     9781 attctcagac ttaatatcta tatgatgaaa gaaattattc cacataaaag aatagtaact
     9841 ctccggatga ctctttggta gattgacgta ttcatccatg ttaaacgtta taacattttt
     9901 gaatgaaact ttaccgcttt tattcaaagc aattaactct ctatacatac caagaggaga
     9961 agatcctgtt ggcaaaccta atacaaatgg ttttgaatct gtaggtttaa acgcattaat
    10021 ttttgccaca acataatttg cagcccattt agatacgttt gcgtaatccg gctcaataat
    10081 taatctcata attttataaa taaatttagc aattagcgcc tggcaaaggt aataatattt
    10141 caacaaattg tacttttgta caaaaaaaat atagctgcaa gagaaaaaaa tacgacttat
    10201 aaataactac acaatctatt agttaacaac tcaaaagcaa tgattaaatg tattacaaaa
    10261 cacaaattta atctgttttt tgattatctt tgtagcttag actaattgtg aaattttaga
    10321 tgcaattcaa atatataatc agccaaagca aaataaaagc ataccttata aaatgtgtgc
    10381 agaataatcg aatcccgcac gctcaaatgt tttggggagc agctggaatt ggtaaattat
    10441 ctcttgcact agcttatgcg caatatctta attgtacaaa tagaacagct gatgattctt
    10501 gcgggaagtg tccgtcttgt atacaatatc aaaaagctgt gcatccggat cttcatttcg
    10561 tatttcctta cactaaaata aagggcaaag aatattgtga tgactttcta cccgaatgga
    10621 gagaaatgct taagaaccaa tactttagtt tacaacaatg gcttgaagca attggagctg
    10681 acaataagca gggaattatt tatagcaatg aaagtgaaga gatcttgaga aagctcagtt
    10741 taaaaagcta tgaatcggat tttaaaacca tgataatatg gttacccgaa aaaatgcacg
    10801 agtcctgcgc caataaatta ctaaagatat tagaggaacc tccatcaaac acagtatttt
    10861 tgctggttag cgagcagcca gagttattac tatctactat tatttctagg gcacaaatga
    10921 tacatattcc gaatatacaa gctgatgata tgaaggatct gtttcctccg gagatagtac
    10981 gcacagcaaa cggaagttat ttacaggcta aaatgctttt agaccaaaaa gaagaatcac
    11041 aagcaaactt cattttgtat agaatgttga tggcttatgt gtttaaaaaa gacctagtta
    11101 gcttgaaaaa gttttctgaa gatatggtcg ctaaaggtag agaacctata aaaagtttct
    11161 tagaatatgc acaacacgta acaagagaat gctttattgc aaatttacaa gataacacac
    11221 taaattacac atacccatac gaagccgagt ttacaagcaa attcaaacgt tttgtcagta
    11281 tttacaacat tgatgcacta acagaacaat tttcaagagc atcatccgat attgaacgca
    11341 atgtaaatgc aaaaatagta ttcttcgatt tgggattaca attaatgaca aaaataaaat
    11401 catcataata ttatggatta tacactagaa aaaggtaatt gtcgaatgac tgttaatgga
    11461 tgtcgcaaga attctaatca gatgctgagc gttcacgatt ggttagctga cattgcagta
    11521 gatagagaat catgccaata catagaagtt cagtttaaaa acactagaaa aggtttctat
    11581 ttaaactcta acaacctgca acttgaaaag ggtgatttgg tggcagttga atctaatccg
    11641 ggtcatgaca taggtactgt aacattacaa ggatatctag ttgcattaca gatgcaaaaa
    11701 aatggctttg atccacaaaa aaacgaagta aaaagagttt accgcaaagc aaagcaagcc
    11761 gatcttgata aatgggaaga atcaaaagca aaagagcatg atgcaatgct tcgttcgcgt
    11821 aagattgcac aagacttaca tttagacatg aagattggtg atgtagaata tcaaggagat
    11881 ggtaatcgag ctatctttta ctatatagct gatcagcgag tagattttag agagttaata
    11941 aaagtcttgg ctgatacgtt tcatgtgcga atcgaaatga agcaaattgg agccagacaa
    12001 gaggctggac gtattggagg tacaggacct tgtagtcgcg aattatgttg ctcaactttt
    12061 ttaagcagtt tttcttcagt aggaacaatt gctgctcgta ttcaagatct atcaatgaat
    12121 ccgcaaaaac tagccggaca atgtgcaaaa cttaaatgct gtctaaattt tgaagtagat
    12181 aattatgttg aagcccttaa agaatttcca agcaaagata ttaatttaga gacaggcact
    12241 ggcacctatt atttctttaa gctagataca tttgctcgca aaatgaccta ttcggctgct
    12301 cctaattttc ctaaagactt agtagaacta agtttagaca gagtaaagga aattattgca
    12361 atgaataaaa atggcgaaaa acctgatagc ctaaatgcgc aaacaaaagc tacggctcaa
    12421 cctgtaggat ttgcaacagc tgttggagaa ggtgttttaa cgcgtttcga caaaaagaag
    12481 aaaaagaaca agaggttccc tcgtcaagga aatacaaacg gaaacgaacg atcaacaaac
    12541 aacgaagtca ataatgatga agaataaaat tctatatatt gccggagtgt ctgcattaat
    12601 attattcgga tgcgtcaaaa acacagcata taattcttat aaaggtactg acgtaaacgg
    12661 ttggaaccaa gatacttctg taatatttga tgttgatatt acagacactg tatctgcata
    12721 caatataatt ttaaatgtac gacacacaac tgaatatcca tatcaaaatc tctggatgtt
    12781 tgtacgttcc tgttctcccg atggaattgt acgtgatgat acaactgcat gttttctagc
    12841 cgacaataca ggtaaatggc tcggacaatc atatttctct gtatatgaaa tgcctgttat
    12901 gtttatggat aaaatacgat tccctaaaga aggagtttat catttcgaaa tatatcaagg
    12961 gatgcgtgat agtatgttaa agggtataag agatatcggt attactattg aaaaatccga
    13021 ttcgaaagat gggaaagaat aaattacaga aatttgcaga catggaaaca ttttctcatg
    13081 tctttcagta tccctttggt aaacttcaat ccggagttgt tttcccatac aaaggaaaat
    13141 ggaatagttt ttttggaaac gacaatccaa ttgttttgga attaggttgc ggtaaaggtg
    13201 agtatacggt aggattggca cggaaatatc caaataaaaa ttttattggc attgacataa
    13261 aaggagctcg tatctggaca ggtgcaaaag attcaaacga aacaggcatg aagaacgtag
    13321 catttttgcg cacaaacata gagttactca attatttttt tgcatcggat gaagtgtctg
    13381 aaatatggat taccttccct gatccacaaa tgaaaaaagt gagaaaacgc ttaacatcaa
    13441 cacgtttcat gcaagaatac agagctataa tgcgtaaaaa cggattgtta catcttaaaa
    13501 ccgatagtcc ttttttgtat cagtatacat tgcttatggc acaaaaaaac aatttgtata
    13561 ttgaacaatg cacagacgat ttgtaccata caccaaacat tgatgaaaat ttattatcta
    13621 ttcaaacata ctacgaacag caatggctag aacgtggcat gaatataaaa tacataagat
    13681 taaaattatc atcgtctgca gaatggcttg aacctgacga tataattgaa gaagatgatt
    13741 accgcagttt taatagaagc aggcgcagtg ctaacaatca aagaacaaaa tagacttatt
    13801 aatattatag ctaaaaacaa aaaaatgaca ttgtatccaa aactaatact tgatgctctt
    13861 caacatgtaa gatatccggg gacaggtgaa gatattgtta cagccggaat ggtagcagat
    13921 aatattcgca ttgaaggcaa ttctgtttgg ttatcgctaa tgctaaagcc caacgatcca
    13981 ctacaaaaat cacttgtaaa ggcttcagag caagcaataa taacatacat cggagatttt
    14041 gtaaatataa aaggacatat atctgtaatg caagttgaaa aagaaacaaa aacagcagaa
    14101 gttcctttgt caggagtaaa atgtaaaatc gcagtatcat caggaaaggg aggagtcggt
    14161 aaatcaactg tatctgcaaa tcttgcaata gcattgcaaa aacaaggata taaagtagga
    14221 cttcttgatg ctgatattta tggcccttct ataccaaaaa tgtttggcat agaaaatgaa
    14281 cgaccctttg caaatgaatc aggaatgata gttcctatag aaaaatatgg cattaaaata
    14341 ttatcaatag gatttttcgt caacaaagaa agtgcagtag tatggcgtgg agcaatggca
    14401 agcaatgcaa tcaaacaatt acttaccgaa gcgttatggg gagatcttga cttttttatt
    14461 atagatcttc ctcccggcac aagcgacata catcttacta tagttcaaac aattaaactt
    14521 gatggtgcta ttgttgtaag tacaccgcaa gaagttgctt tagcagatgc ggtaaagggc
    14581 attgatatgt ttcagaacga caaagtggga gttcctattt tgggacttat tgaaaatatg
    14641 gcatggttca caccggccga acttcctgaa aataagtact atatattcgg gaaagatggt
    14701 gtgaaaaaat tagctgaaga acgcggaatt gaattattag gacaaatacc gattgtacaa
    14761 agcatctgcg aatcaggtga taacggcaaa ccaatatgtt taaacaacga tggcattgta
    14821 tcttcggcat ttgacaatgt agcaaaaaat agtatctttg cacaaatatt acaaaaataa
    14881 tgacaacaaa aaatctagta aatacaattg ttggcggcat acaagaacaa aaaggattac
    14941 gtattgtaac tgctgactta acaaaaatac ctaataccat ttgcaaatat ttaattatat
    15001 gcgaaggtaa ttcaaacacg catgtaaact caattgcact aaaaactaaa gattacgttc
    15061 gcgaacacgc caaagaaaaa ccaattgcaa tggagggatt tgaaaattgc gaatggataa
    15121 ttgccgatta cggagacata atgttgcata ttatgcaacg tgaacctcgt gcattctacg
    15181 atttagagca cttatgggca gatgcaaaat taacagaaat cgctgattta taatatggaa
    15241 aacaaacaac agcctcaaaa aaacccgaac aaccaaccac caaaatttcg ctttaacatg
    15301 tattggctgt acggagctat tgcagtcttt ttaattgcaa taactttcat aaatgaggaa
    15361 tctccaaaga aggatgttcc atattctaat tttcaagaat atgttgaaaa aggatatgtt
    15421 gaaaaaatag tagttattac caataaaaat gaggccgatg ttagtttaaa agacgatcca
    15481 caagcaataa aaattgtact tggtgaggga ggagaaaaac tatatccaaa gaaacctcaa
    15541 ttaacagttc gtataccatc tcctgaaaaa ctagacgatt ttattacggc aaattcagca
    15601 aaaaccggag agaaaattga tgtcagatat gaggaaagaa aagattattg ggactcccta
    15661 atatggtcgt ttggtcctat cctgcttatc gtttttattt gggttattgc aatgcgtcgc
    15721 atgggaggtg gaggtgcagg tggcggcata tttagtgtgg gtaaatcaaa agctcaatta
    15781 tttgacaaag gcacagctgc aataaaagta acattcaaag atattgccgg tttggcggaa
    15841 gctaaacaag aggttcaaga aatagttgat ttcctgaaaa acccggctaa atacacttct
    15901 ctgggaggga aaattccaaa gggtgcccta cttgttggcc ctccaggaac aggtaaaaca
    15961 ttattagcta aggctgtagc cggagaagca aatgttcctt tcttctctat gtcaggatct
    16021 gatttcgtag aaatgttcgt aggtgttggc gcatcacgcg taagagattt attccgccaa
    16081 gctaaagaaa aagctccatg tattattttc atagatgaga ttgatgcagt aggtcgcgca
    16141 agaggtaaaa atccatcctt caactctaat gatgagagag aaaacactct gaatcagttg
    16201 ctcacagaaa tggacggatt cggttctaat agtggagtta taatacttgc agcaactaac
    16261 agagctgatg tattggataa agccctactt cgtgccggac gttttgacag acaaataagt
    16321 gtagacttac cggatattca cgaacgcaat gaaatatttc aagttcattt acgtccacta
    16381 aaattggcaa aagaccttaa cgtagatttt ctcgcaaaac aaactccggg tttttccgga
    16441 gctgatattg ctaatgtctg caacgaagct gccttaattg ctgccagaca taacaaaaca
    16501 gaagttgaaa aacaagattt tctagatgct gttgacagaa ttattggagg tcttgagaag
    16561 aaaaacaaga ttacaactgc atcagaaaaa aaatctattg ccactcacga agccggacac
    16621 gcatctgtta gttggttgct tgaatttgca aatcctttag ttaaagttac cattgtacca
    16681 cgtggtaagg cactaggagc agcatggtat ttacctgaag agagacaatt gactactaaa
    16741 gaccaaatgc ttgatgaaat gtgcgcactt ttgggaggta gagctgctga ggaggttgtt
    16801 tttggaaaag tatcaacagg agccttaaat gacttggaaa gagctacaaa aatggcatac
    16861 tcaatggtag tttactatgg tatgagcgat aagttgtcta atataagtta ttatgactcg
    16921 acacaatcag actactcatt cacaaagcca tacagcgata aaacgtctga aattattgat
    16981 aacgaaatta agagtatagt aaactcacaa tatcaaagag ctttaaccat aattacagaa
    17041 aatcgcagca agcataaagc attagctgat ttattagttg aaaaagaagt tatctttgct
    17101 gaggacgttg aacatatttt tggaaaacgc ccttgggcca gtcgcatgga agaaattatt
    17161 aaggaaagtg caaagaacga tgagagtaaa cctattatag aagatgaacc tcaaaatacg
    17221 gaggacacaa gcaatgaata atttttggca acgcacacta accgggattg catttgtagc
    17281 agtcatactt acaagtattt actttcaaga taaatatttc attttcactg ggatatttgg
    17341 cttagtaaac atattagcac tatatgaatt ttacaaaata acaaacatta gtgacagagt
    17401 aaatgtgcct accatatatt taattatttg cggtacaatc ctatacttcg gatgcttttg
    17461 gcttgcaaaa tacgggggag aaattgttat agtatgctat gcaatgtgta ttgtagccct
    17521 actgattgca gaattataca gaaaaaaagc agctccaact cacaatattg ctttttcgct
    17581 tatgggacaa gcaattgtag ctgttccatt cggcttacta aattttattg cgattcaaca
    17641 aaacgtatat tggttgtatg ccctatttat tctaatctgg acattcgaca ccggagctta
    17701 tgtatttggt gtaagttttg gaaaacataa gatattcccg cgtatttcac ccaaaaaatc
    17761 atgggaaggc gaaataggag gagctatact cactattatc ggatcgttaa tatttgcgta
    17821 cttcacaccg gaagttgcta tttggaaatg gattgttttt ggttctttga ttattatttt
    17881 tggaacatac ggagatttgt cagaatcttt gcttaaacgc acagcatcac ttaaagactc
    17941 cggtacaatt ttacccggac atggtggaat gctagatcgt tttgatagtt taatgctgat
    18001 aattccggtt atttacattt accttcaatt tatagaagaa atatgatacg catccataaa
    18061 gaaggtaaaa atatgttgct tgtactacta tttacgctca tcatcgtaaa tggtttgctg
    18121 ttttacatat ttttaacacc aaccgttcca ataatagtag ctgtactttc ggttggctta
    18181 tttatatttt tcacacactt tttccgcata tccaacagaa tattagttgc tgatgatact
    18241 tatgtagttg cacctgccga tggaaaaatt gttgtgatag aaccggttat ggaaaatgac
    18301 ttactaaaag aaaagcgcat tcaagtgtct atttttatgt ctgtttttga tgttcacgca
    18361 aattggtacc ctatatcagg aaccgtgaaa cacgtatcac accaaaatgg ccgaaaatta
    18421 gcagcttatc ttccaaaatc cagttcagaa aatgaacgtt cgtcaattat aattgaatca
    18481 acaaataaac aaacaatatt agttagacaa attgccggtg cattagcacg ccgtattgta
    18541 acatatgcca aagttggtga aaaatgtaca ctaaacagtc aacttggatt tattaaattc
    18601 ggttcaagag tagatatgtt tttacccttg gatgcagaaa ttcttgttga tttgcatgaa
    18661 gaaactgttg gcaatgaaac actgattgca aaattgaagc atacaagtgc agaataaatt
    18721 gtaaaccact aaggtttata atacacatct taatttatta atttttatta ctacccaatg
    18781 ttggtaaaat cattcggggc agcggtgcaa ggtatcgatg ccacaattat tactgttgaa
    18841 gtaaacgttt cttcaggtat taaattcttt ttagttggct tacctgataa tgcaatcaag
    18901 gaaagtcacg aacgtatcat gtctgccttc ggacataaag gatacaagtt tcctagacaa
    18961 caagtaatta taaatatggc acctgcagac atacgaaaag caggttcatc atacgacttg
    19021 ccaattgcaa tgggaatttt ggctgcttcc ggagaactgt cagagaaatt acttaacgaa
    19081 tatgtaataa tgggtgaact atcactggat ggaaaagttt tgcctataaa aggagcttta
    19141 cctattgcag tgaaagctcg agaagtcgga ttcaaagggc tgattctacc gaaagaaaat
    19201 gcccgcgaag ccgcagttgt gaacgaattg gatattttag gcgtagatga cattagtgag
    19261 gtagtcgatt ttttaaacga aaaaacaaaa atagaaacca caatagtaaa tactaaagag
    19321 gattttttaa atcatgtaaa taaagatgag cctgactttg cagatgtaaa aggtcaagaa
    19381 tcagttaaac gcgcactgga agtggctgca gcaggtggac ataatattat attaattggc
    19441 cctccgggag caggaaaatc gatgatggct aaaagaatac catcaatact tccaccatta
    19501 tcacttagcg aagcattaga aacgacaaaa atacactctg ttgcagggaa attagataaa
    19561 acgacttcct tactacatca tagacctttt agaagtcctc atcatacaat atcagacacc
    19621 gctctagtag gtggtggcac atatcctcaa ccaggtgaaa tatccttgtc acataatgga
    19681 gtactatttc ttgacgaatt acctgaattt aagagatctg tattggaagt tatgagacag
    19741 ccattagaag acagaaagat tacaatttca cgcacaagtt gcagtatcga atatcctgca
    19801 agttttatgc tagttgcatc tatgaatcca tgtccttgcg gttattataa tcatcctgaa
    19861 cgtccttgtg tttgtgcacc cggagctgtt caaaaatatt tgagccgaat ttcaggtcca
    19921 cttttagatc gtattgatct tcaaattgag attactccaa tttcatttgg acaattgtca
    19981 aatagaaatc aatcagaatc aagtgccatt attcgtgaga gggtaataaa cgctcgcaac
    20041 atacaagaaa aacgatataa gaattcaaat ggagtgcact gcaatgcaca aatgactgtt
    20101 aagctattac acaaatatgt tcaacccgac gaagccggcc tcaaaatgtt acaatatgca
    20161 atgtctaaac tgcacttgtc agcaagagca tacgacagaa tccttaaagt tgctcgcaca
    20221 atagccgact tagataattc agaaagagta acagcagaac atttatccga agccatagga
    20281 tacaggaatc tagaccgcga aggttgggga ggctgattac aactcctcta tcaatccttt
    20341 cataattgca gtcattttag gctgaacgct atcggcaatg cgttgcactt cttcgtgcga
    20401 taccgtgtcg acaatacctt ctatgcctaa atctgttatg atagaaattg caaatacacg
    20461 tatacctgag tgaactgcta ctattacttc aggtactgtg gacattccaa ctgcatcacc
    20521 acctatacgc ctaaaatatc tgtactcggc aggagtttcg aaagtaggac ctgaagtacc
    20581 aacatagact ccttccttta cttgaacacc gagttgttgt gcgattttct tcgcttttgt
    20641 tcgcaaatct tgatcgtatg cagcactcat atcagggaaa cgaggcccta actcattaaa
    20701 atttttgcct cgcaatgggt tttccggaaa caaattaata tgatctgata ttatcatcaa
    20761 atcacctatt ttaaaatcag gattcatacc tcctgcggca ttcgaaacca ataatatttc
    20821 aatacctaaa gacttcatta cccttacagg gaatgttact tctttcatgt cgtatccttc
    20881 gtaataatgg aaacgacctt gcattgcaag cacacgcact gctcccaaac taccaataat
    20941 taattttcca ctgtgcccct caactgtaga taatggaaag ttaggaatac ttgcataatt
    21001 tatttcctgg cggttagtaa tttgtgtagc aagattaccc aaacctgtac ccaatattat
    21061 gccaatcttt ggaatatcgt ttattttggt ctttaggtgc gacgatgttt cgtttattct
    21121 tttcaacata atcaataaat ttattattaa aattatcttg tttcccttgc aaaattttta
    21181 tctgcaacgg aatggcatat atatacgatt ttaggtcatt cggaatatat ttgcacccta
    21241 ctaatttagc agcatccttt tctgtaacaa atatatattt attaggtccc gacaacaaac
    21301 ggaatctctc agaaatatca caccaatcct tttcacttaa taaataatga tctgaatatg
    21361 atacagattg tactatttta tattgttctt ccaaatattc tttcaagggt tttggctggg
    21421 ctattgccgt taccaataag actgatccaa cttcctttaa ttcaccgctt atttctttaa
    21481 atacagctac cggtttttga tatgtatatg tcgaaaaaaa caaagactga aatggtctga
    21541 gatataattt ttttcgtagt attcttactt caagcggact aagatctata gggcattttg
    21601 ttactactat aatatctgcg cgattttttc cgctagcagg ctcccgcaaa tcaccatatg
    21661 gcaacaattt atcttcagtt attatcctat tataatctac aagtaacaca gataaaccgg
    21721 ccttaacata cctatgttga aaggcatcat ccataactac cacatcaatg tttgaataaa
    21781 tagactcaat ttctcgtaat gcctctcttc tatctttatc aacgacaact ttaacttgtg
    21841 gaaattttga atatacttgg tatgcttcat ctcccaaaat tgacgcattt gcattaatcg
    21901 tatttgcaaa aactaaacct ttagatttac gtttatagcc acgactcagc attgctgtat
    21961 taaatttatc agataagata tgcagtaaat attcaacatg tggagtcttt ccggttccgc
    22021 ctactgtcaa gttaccaaca gaaatgatcg caattttata ctttgtactt ttgatgattt
    22081 ttgcatcaaa caaataattg cgtatcataa ctaccaaacc atatatccaa ccaatgggat
    22141 aacagacaat tcgtaaacat ttgaagaatt gtgatttcat agtggtacaa agttagtttt
    22201 tttgattgga aattcattag aaaagaagta cttttgtagt gaattagaaa attttacgtt
    22261 tatggaatct attaattggt ctgaactttc gttcggttac ataaaaacag actgcaatat
    22321 tcgctgtgaa tatcacaatg gtgcatgggg acaattagaa acccatacag acgagtatat
    22381 taatatgcac atggctgcaa cttgcttgca ctatgcacaa gaagcatttg aagggctgaa
    22441 agctttccgt ggtaaagacg gaaaaatcag aatattccgt atcgatgaaa acgaaaaacg
    22501 attagaatcc tcttgcaatg gaatcaagat gccgtgcttg cctaaaggat attttcgtga
    22561 agcagtatta tctgctgtta aaaaaaatgc acgttttgtt ccaccttacg aaagtggcgc
    22621 atcactatat attagacctg tattgttcgg ttacacacca caaattggtg taaatccggc
    22681 caaagattat ttatttattg tttttgtatc accggtaggt ccatatttta aaggagggtt
    22741 ttcaactaat ccttatgtaa ttacgcgtaa tcatgatcgt tctgctcctc taggtacagg
    22801 tatatataaa gtaggtggca attatgccgc aagtttagct gcacacgaag aagcacacga
    22861 aaaaggatat tgttcagaat tctatttgga tgcaaaagaa aagaaatata ttgacgaatg
    22921 tggtgctgca aatttctttg gaattaaaaa caatacatat ataactccaa aatcaacatc
    22981 tatcctacct tcaataacta acaagagttt gatggtatta gctgaagatt taggattaaa
    23041 ggttgagcgt cgcccaattc tggaagaaga acttgacagt tttgaagaag caggagcttg
    23101 cggaacagcg gctgtaatca gtcctatcga acgtttggat gatatcgaca ataataaatc
    23161 atacgttttc tcgaaagatg gaaaaccagg tcctatttgt accatattgt ataacaaact
    23221 acgtgctata caatatggag acgaggctga cattcataat tgggtaacag tattagatta
    23281 atttaaaaca ataataagat gaaacttctc attttaaatg gtccaaattt aaatttgctt
    23341 ggcacacgcg aacctggtat atatggtcaa gaggggtttg atacttattt aacaaaacta
    23401 aataagcgat tccctaatgt ggaaatcgct tattttcaat caa
//
LOCUS       DAOPID010000004        22134 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_2470_length_22134_cov_5.686082, whole genome shotgun sequence.
ACCESSION   DAOPID010000004 DAOPID010000000
VERSION     DAOPID010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 22134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22134
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2470_length_22134_cov_5.686082"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="PK641_00425"
     CDS             complement(<1..657)
                     /locus_tag="PK641_00425"
                     /inference="COORDINATES: protein motif:HMM:NF024393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3875 domain-containing protein"
                     /protein_id="HPG54853.1"
                     /translation="MKINSHNIEKCLPILCWENSCLVSKTADITVPFEITLPEVYSLG
                     CADFENLHQVWVRALRSLPDYCIVHKQDWFINSEVVSEQVSSSFLSGASAKHFKSRKC
                     LNHRSFLYITLSSKNNMSTSSYYSILCRSRIVPIEMENLNHINDFFDAVKQFCTILKE
                     CGVSTRRLEESEMIGDKSHSGIIEQYLSLDFSEKCVLVDLYTQEKDWMVGDKYVSCFS
                     I"
     gene            complement(975..1283)
                     /locus_tag="PK641_00430"
     CDS             complement(975..1283)
                     /locus_tag="PK641_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002980936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4134 domain-containing protein"
                     /protein_id="HPG54854.1"
                     /translation="MKTPKKIVFSTVLLCINVYDILAQGNGVAALEQANQEIKSYIEP
                     ITNLFYVVCAIVGFIGAVKVYGKWSSGSPDTTRTAASWFGACVFAIVAVTAIRAFFIG
                     "
     gene            1853..3085
                     /locus_tag="PK641_00435"
     CDS             1853..3085
                     /locus_tag="PK641_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HPG54855.1"
                     /translation="MLNYIWAGFFIIGFIVAICRAIFLQDLGVFERIITGTFDMSIFA
                     VMDIALPLAGVMTLWLGLMNIGEKAGAIRFMSRIVGPFFSKLFPEIPKDHPANGQLLM
                     NFSANMLGLDNAATPLGLKAMQSLQDLNPKKETASNAQIMFLALNTSGLTIIPIAILA
                     QRAILGSANPTDIFIPLLISTYISTLTAILFVGIKQKLNLINKTVIGWLSGITIAIGL
                     LCWYVTTLNKEEMMTFSSIFSNSLLLIIIVAFISGALYKKVNIFETFIDGAKSGFETS
                     VKIMPYMVGMLVGIAVFRASGALDLIVLGIQKIVLLFSDHTEFVNALPTAFMKPLSGS
                     GAKAMMVECMRTYGPDSFVGRLSCLFQGAADTTFYIVALYFGSVGIKKTRYAIGAGLI
                     ADLAGVIAAIASAYLFFG"
     gene            3178..3612
                     /gene="ybeY"
                     /locus_tag="PK641_00440"
     CDS             3178..3612
                     /gene="ybeY"
                     /locus_tag="PK641_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HPG54856.1"
                     /translation="MMISYQTEGVEKPKICTRRTSKWIKEIANSHNKKIGEIAYIFCS
                     DEKILQINKEYLKHDYYTDIITFDYTEDDKISGDIFISLETVKSNSEQFGTDYYDELS
                     RVIIHGILHLCGFKDKEPSAKAKMTDEENKALSAMSEKGIKK"
     gene            3636..5510
                     /gene="mnmG"
                     /locus_tag="PK641_00445"
     CDS             3636..5510
                     /gene="mnmG"
                     /locus_tag="PK641_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HPG54857.1"
                     /translation="MNFDYDVIVVGAGHAGCEAARAAAKLGSRTLLITMDMAKIAQMS
                     CNPAIGGIAKGQIVREIDALGGGIGYVTDRSSLQFRMLNLSKGPAMWSPRSQCDFTKF
                     ILTWRYLLEQTENLNIWQDTVSQLIISNNKVVGLKTSLGAEFKAKSVVLTNGTFLNGL
                     IHVGKVQVKGGRMSEPASTGLTEQLTSIGFTSQRMKTGTPVRIDIRSVDLSLTEIQNG
                     DCDFHKFSYLDYEPRNLKQLPCYLLNTNEEVHNVLRSGIKDSPLYNGQIKSIGPRYCP
                     SIETKIITFPDKQTHPLFLEPESEESTEYYLNGFSSSLPMDIQINALQAIPAFKNIHI
                     FRPGYAIEYDYFEPTQLTHSLETKQIQNLFFAGQINGTTGYEEAAGQGLIAGINAHQK
                     CHGGSEFTLQRNEAYIGVLIDDLVTKGVDEPYRMFTSRAEYRILLRQDDADARLTTKS
                     HKIGLADNTRYKLWKEKEKQCAILKDFLETTTASPAEINPLLERLGNSPIKQGAKYKD
                     ILLRPDLSITKMAEELPLLKLKIDEILNRKEEITEAVEISVKYAGYISREKLIADKLQ
                     RLEEIKIKGKFEYSSIQAISIEARQKLTKADPETIAQAARIPGISPSDINILLTLMGR
                     "
     gene            5574..6098
                     /locus_tag="PK641_00450"
     CDS             5574..6098
                     /locus_tag="PK641_00450"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HPG54858.1"
                     /translation="MTIEEVKKEIRDVPDFPQKGILFRDFTTAFKKPECMKWFENRLV
                     DMYSNLNITKVVGIESRGFIMAPILANKINAGFVPIRKKGKLPANVIEISYAKEYGVD
                     TIQIHEDALSSDDIVLLHDDLLATGGTMEAAYELIKKFGVKKVYINFLAELDGLNGRR
                     LFDANAQIDSLIHY"
     gene            6107..7939
                     /gene="uvrC"
                     /locus_tag="PK641_00455"
     CDS             6107..7939
                     /gene="uvrC"
                     /locus_tag="PK641_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HPG54859.1"
                     /translation="MSTRLEHIKQIVSSLPETPGVYQYYNEDGLIIYVGKAKNLKRRV
                     SSYFNKTQTSAKVNVLVKKIYDLKYIVVNTEADALLLENNLIKKYQPRYNILLKDGKT
                     YPWLCITKEEFPRVFKSRQVFRGAEYFGPYSSVWVLDTLLELIKKIYPIRTCRLPLKE
                     DSIAAGKYSVCLQYHIHNCLGCCVGYQKKDDYQKMIAEIKEIAKGNSRAITDYLLKEI
                     QNLAGNYQFEKAQELKLKYDALLQYQSKTIITTTHDDDLDVFGYEEYDNIAYINILRI
                     SKGSIIQGFTIECHKKLDESKEELLAIAILELRSRLNSNSKQIIVPFLPELELNDTQI
                     SIPQKGDKKKLLDLSLQNVKQYRIDRLKQSEKLNLEQRSTQILKDLQNALHLDRLPMH
                     IECFDNSNISGSDAVAACVVYRKAKPCKSDYRKYNIKTVQGPDDYASMREVVFRRYQR
                     MINENTTLPDLIIADGGIGQMNAIRDIVVSQLGLSIPIAGLAKNDKHQTNELLFGFPP
                     QAIGLKPTDNLFKFLSGIQDEVHRFAISFHREKRSKTQIHSELDEIKGIGEKTKSLLI
                     LHFKSVKRIQTAEKIELAEIVGNARASIIYEHFHPILTAPELKI"
     gene            7952..8407
                     /gene="dtd"
                     /locus_tag="PK641_00460"
     CDS             7952..8407
                     /gene="dtd"
                     /locus_tag="PK641_00460"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HPG54860.1"
                     /translation="MRAVIQRVKHAKVEVDKKIVGEIKTGLLVFVGIEDSDTEEDENY
                     LAQKIVNLRIFNDRQKVMNLSVLDIDGEILCISQFTLMAITQKGNRPSYIRASKGLIA
                     MPIYEDFCKKLSELLKKEIKKGIFGADMQIMLQNDGPTTIWMDSKSKAY"
     gene            8437..9309
                     /gene="deoC"
                     /locus_tag="PK641_00465"
     CDS             8437..9309
                     /gene="deoC"
                     /locus_tag="PK641_00465"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HPG54861.1"
                     /translation="MKDFEGLLKNYPFNGTENKIKSEAKRIIDEHYENNDTSASLKLI
                     FSCLEITSLKSTDNEKTIESLVEKVNLLNEKIPELPKLAGICVYPVCVETVKSLLIEE
                     IGITSVIGFPSSQTFTAVKLAESALAISDGATEIDMVLPVGQFLIGNYDYIFDEIQEI
                     KAAVHEYTLKVILETALLNTYENIFKASIIAMEAGADYIKTSTGKEGSTDIYSVYTMC
                     LAIKAYYEKTGIQHGIKIAGGINKTQDALLYYTIIKDVLGEEWLNNEYLRFGASRLAN
                     ELISGILKEENNYF"
     gene            9367..12240
                     /locus_tag="PK641_00470"
     CDS             9367..12240
                     /locus_tag="PK641_00470"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HPG54862.1"
                     /translation="MKRLLTVLIVFLSILASVSATIRQPIQNFYKMDYKLGAQNWGVS
                     QQRNGLMYFANMNGLLSYDGIRWTSSQFPGSGNNVRSLKITPAGDIYVGAYNEFGVFK
                     AQRNGLSEYESLSALLPEKDLTFGEIWHISILNNSIIFQGTNQIFFYENGQITKLDTP
                     AKIKFSAVFNNALYLTSDTDGVFVLIGTSFISLPNCEELQNKSVCAILPMEDNAIAFI
                     TESHGIYDYQNGEMKKSTLPINDILIKYQAFCAEINGQTLAVGTVQNGVVVLNLKTKE
                     YTIINMDSGLQNNTVLSLDFDMEGNLWLGLDKGIDYVKINSPFRSLFAQNNPYGSGYC
                     SLIVGSDLYLGTNQGLFVTKYANSEIGAIETIDGTQGQVYAIKQIDNHIYCCHHKGLF
                     EINGKYSRKIANIEGVWTIQKLRSNPNYLIAGHYNGFFLMKKQDGFWVFSHNIEGFNE
                     SSRLFEQDEAGDIWMSHGLKGVYRIHLSKDLKKAESVKFYGTEKGFPTNEHISVFSVN
                     GDLIFAAEDGLHRYNSATDFMDVNTQLTNSLLGGGQYYSLKEYQDDRLWFVKFDQIGL
                     AIKQKDDVYTCNPNKCFSIPEELLYEYFDLNALDSENILVSNEEGFTLVNLEMLDKTN
                     LFDVFIRQIYSTKSEKNITSQYLKTTEEFAPIKIPYSDNSLRFTFGTVSFSSNKNNQT
                     LYSTRLLNYEDNWSKPNLSSSREYTNLHEGTYTFQVKTFNRNSESSFTEYTFTISPPW
                     YRSFWAYAIYFLLLIGLLLLLRKYIQYKESLLENENKKQMEAQKKLHEEESAEKEKEI
                     IALKNEQLSSELVYKSNELASSTMNLIRKNEVLIDIKDEISKIAGGLSNSEEGKSISK
                     KLTRLVHSINSNIEHDDDWMKFEQNFDNIHQDFMKRLSEQYKGLTVSDKKLCAYLKMN
                     LVSKDIAPLLNISVRGVEIGRYRLRKKLNLDRDTNLTEFLQNF"
     gene            complement(12301..13401)
                     /gene="ychF"
                     /locus_tag="PK641_00475"
     CDS             complement(12301..13401)
                     /gene="ychF"
                     /locus_tag="PK641_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HPG54863.1"
                     /translation="MSLQCGIVGLPNVGKSTLFNCLSNAKAQAANFPFCTIEPNVGVI
                     TVPDERLTALAELIHPQRIVPTTVEVVDIAGLVKGASKGEGLGNKFLANIRETDAIIH
                     VLRCFDDANVTHVDGSVNPVRDKEIIDTELQLKDLETVESRIIKVQKQAQSGGDKTAK
                     TILEILFKYKEVLLQGKSARTLVLDKEERKLTKDFCLLTDKPILYVCNVDEASAVNGN
                     HYVDELKASLKDENAEILIVAAATEADIAELETYEERQMFLEEVGLKESGVSRLIKAA
                     YKLLDLQTYLTAGEPEVRAWTFRKGWKAPQCAGVIHTDFEKGFIRAEVIKYEDYISLG
                     SESACKEAGKIYVEGKDYEVQDGDIMHFRFNV"
     gene            13476..14375
                     /locus_tag="PK641_00480"
     CDS             13476..14375
                     /locus_tag="PK641_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG54864.1"
                     /translation="MKNSSKNIPIIISIILSIAVIALGVLFFLQHKDLQEKTQELSSV
                     TAEMEFEKQQSVEEFEQIAHEYENFYINTSNDSLLKLIDNEKQKVQQLLQELKTVKAT
                     NTRRIGELKKELSTVRGVLKSYVEKVDSLNALNQNLQKENYQVRQQYEQTAKELEQKA
                     AEAAELDKKVSMAAILEASNINISLLNEKGQPTKSLRKTASFEICFDILRNITAERGK
                     KMVYMRITDYKEELMTLPGQNTFNFEGNQIEFSGKKEIEYGGEAVHVCIYYPIQEKLT
                     TGTYSIAIFMEGNLIGSKSFAIK"
     gene            14378..15481
                     /gene="hemW"
                     /locus_tag="PK641_00485"
     CDS             14378..15481
                     /gene="hemW"
                     /locus_tag="PK641_00485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HPG54865.1"
                     /translation="MAGIYIHVPFCTQRCSYCDFFSTIRLEEMDSYTDSVCLEIIQRK
                     DYIKDKHINTIYFGGGTPSLLSKENLTKIFDTLYKNFDFSNLKEVTLECNPDDIRKDF
                     FCKISDTPINRLSIGIQSFQDDELVMLNRRHNAEQAKKAVKMAQDNYFNNISIDLMYG
                     LPQQKIDLWNKTIQEALSLNVQHISAYHLSYEVGTKMYSMRNKAVDEETSLELFQSLR
                     HSLIGGGFEHYEISNFAKTEFRSIHNSSYWKNVEYLGLGAGAHSYNGCNSRQWNLSNL
                     DKYQQAVKNNGIYYEQEILNDDDIYNELIIKSLRTCEGININNIDNKYLDHCIKNSNL
                     YTKEGLLCKQDNFIKLNENGIFVSDRIIRDLMI"
     gene            15478..15876
                     /locus_tag="PK641_00490"
     CDS             15478..15876
                     /locus_tag="PK641_00490"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HPG54866.1"
                     /translation="MNIIKAYWKTSLISIIIIYLSMIKMPSSIEIPKIPYYDKWVHLI
                     MYALLCTIVLFDSIKNLHRRPYIPAAVYSIILGGILELLQILFPPRSASFADFLANLL
                     GCSLSLIIFFLIKFIHGRTTNSKNHCINSD"
     gene            15830..16936
                     /gene="dprA"
                     /locus_tag="PK641_00495"
     CDS             15830..16936
                     /gene="dprA"
                     /locus_tag="PK641_00495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPG54867.1"
                     /translation="MEEQLIQKTIALTLIDGIGTQNTKKLIDYFENADNVLRQSARSL
                     ASLSGIGQSKAESIVSQFNDVLKKAEVELKYIYDNRINLHFYKDSNFPKKLLECSDCP
                     VLLYSKGHFDFENGKYISIVGTRNATEYGKKLCQDFVRDVSIRQADTTIISGLAYGID
                     ICAHLSAIENDLPTIAIMAGGFAHIYPGAHKHVAELMVEKGGIITEGISSTFPERQNF
                     LKRNRIIAGLADAVVVVESAAKGGALVTADIAHSYNRDIFTFPGRVNDTWSKGCNQLI
                     KQQKAALIESFSDLEYLMSWDKNKIKYIQTSLFNDLTEKEQAVYSIIKKEKKLHKDLL
                     NQFFENETSLQSILLSLECKDIIKCLPGNFFIPL"
     gene            17561..18484
                     /gene="nadA"
                     /locus_tag="PK641_00500"
     CDS             17561..18484
                     /gene="nadA"
                     /locus_tag="PK641_00500"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HPG54868.1"
                     /translation="MDFIKEINKLRKEKNAVIMAHYYQSGDIQDIADNVGDSLQLAQW
                     AAKTDADIIVLCGVHFMGETAKILSPNKKVLIPDIEAGCSLADSCSADEFEKFIKKHP
                     NHRVISYVNTTAAVKALSDVLVTSTNTRNIVESFNKDEKIIFGPDKNLGNYINSVTGR
                     NMVLWNGACHVHEKFSVEKIAVLKKEYPNALVLAHPECKATVLAMADFVGSTAAILNF
                     AGKSEKKNFIVATESGILHKMKLSYPNKQFIPAPPDDEICACNECSYMRLNTMEKLYK
                     CLRDENPEIKIDPEIQEKAVKPILRMLELSK"
     gene            complement(18486..20228)
                     /locus_tag="PK641_00505"
     CDS             complement(18486..20228)
                     /locus_tag="PK641_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPG54869.1"
                     /translation="MNLNEINREYLEQFPFLNETQIENLLEYRFDYGDFQSIYELSLI
                     KNFDDETISNCLPFLIVYAKADLEKVSLKNIFKFAKHRITIRADYGFPQKKGYTNGAY
                     LGKAWASYFKYAFTHKNASIAVSMDKDAGEPFDWKYNKGFDFYSGHISIKDIKHCKLM
                     IIGDFKANFGQGLIYKSNSYHINSTDLAALCQRQDGFQPFFSVSEQGFLRGFATTLQF
                     NKIQLSAFASHTQYNSENYHRTISSFIKKNTNNSYSLGFNALYRHKFFKIGSSFCYEF
                     LSKKINVGLDYRAKIKRFYIAGEFAVNEKGGFATINHINFLMNEKLSFSALYRYYDLN
                     YNSTYGCAYSESGLNDEQGLLIGFEIRPFRYWKISAFADVFAFKSFKYQIKQPSEGFK
                     TFCQLIYTPNNNHYILLRYNCKQMSVNDNDDNLSHTQTVTNYRKHSLKFTYQAKLSDF
                     FTFQTGAQSNIYIKNSNIDYAWLLYMDFNYIFIKFPLQISLKCAFFDVPLYENRIYCY
                     EKDVLFAYSSPSYSGQGFRNCLMLKYMPSAQWSIYIKSALNFYTDRETIASANEEIIG
                     NKKIDLRLLVSFKF"
     gene            complement(20408..21235)
                     /locus_tag="PK641_00510"
     CDS             complement(20408..21235)
                     /locus_tag="PK641_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG54870.1"
                     /translation="MKRSFFLLLIIFPHCSYAMELGNRIDPVGLAIGKTNASANINQN
                     YVPYNQAVAALCEFPIIQTTYTNSFFLPSLGRGQLGLVYPCKFGTWCASIDYYGFSEY
                     GEISAGIAFAKWFKPYFSIGLELDYIGIFLSKDDGVASTAIVQLGMIVFPTNSLNISV
                     HAYNLSFSPVHCTNLKIRIPSIFKIGVSYKFEKNILLKFELSKELSSPILIGGGIEYE
                     PIKFVTIRAGLCGQKNLVSTIGIGLNYKSFGCDLGISYDTKLGISSAFGLYYRLKKR"
     gene            21301..21909
                     /locus_tag="PK641_00515"
     CDS             21301..21909
                     /locus_tag="PK641_00515"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HPG54871.1"
                     /translation="MKNKLIILFITATILSCGSVCAQNYYYSESLHGYILPIAVYGTD
                     TIPWVELREVPIYPPIKNKKQEKFYWKTVRDVRKVYPYVKIVGGEYARINSMFDTIQD
                     VKIRKKFMKKYEKELLKKYEPILRTFTLSQGKMMIKLIDRETNKTSYTLIKEFRGGFV
                     AWWWQLFAKLVGADLKEDYDFSKKEKDRIIERVITLYEAGLL"
     gene            21955..>22134
                     /locus_tag="PK641_00520"
     CDS             21955..>22134
                     /locus_tag="PK641_00520"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537985.1"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPG54872.1"
                     /translation="MAKELTPRSENYSQWYNDLVVKADLAENSAVRGCMVIKPYGYAI
                     WEKIQRQLDQMFKDTG"
BASE COUNT         8164 a         3573 c         3638 g         6759 t
ORIGIN      
        1 aatagaaaaa caacttacat atttatcgcc taccatccaa tctttctctt gtgtgtataa
       61 gtcgacaagc acacactttt cagaaaaatc gagacttaag tattgctcaa taataccgga
      121 atgtgattta tcccctatca tttcactctc ttccaatctt cttgtagaaa ctccacattc
      181 ctttaatatc gtacaaaatt gtttcactgc atcaaaaaaa tcattaatat gatttaaatt
      241 ttccatttca atagggacaa tcctacttcg gcaaagtatt gaatagtaac tacttgtcga
      301 catattattt ttagaagaca aagtaatgta aagaaaactc ctatgattta ggcatttacg
      361 tgatttaaag tgttttgcag atgctcctga taagaaactg gaagatacct gttctgatac
      421 aacttcacta ttaataaacc aatcttgttt gtgaacaata caataatcag gtaaacttct
      481 caatgctcta acccaaactt gatgaaggtt ctcaaaatca gcacaaccaa gtgaatatac
      541 ctctggtaaa gtaatttcga aaggcacagt tatgtctgct gttttactta ccaaacatga
      601 attttcccaa cacagaatcg gcaagcattt ttcaatattg tgcgaattaa ttttcataaa
      661 aaatttaaat tatattttta attcgcttat tgttacaaac tctttgggta aggtttttat
      721 atgcaaaaaa ttgcgtaagt ccattttctc caaatctctt attcaaataa aagcatataa
      781 acaaggataa taatcctata attagtgctc ctgataatgc aatccactga ttaaatataa
      841 gagaagaaat aaaaaatatg acgaaagaaa gaacaagacc ggcaacagca tacaaaagat
      901 attgcgctcg caaacctttt acttcaacag atcttccaac tcctttgttt attgaatacc
      961 tgctcatctg ataatcaacc tataaagaat gcacgaatag ctgttactgc cacaatagca
     1021 aaaacacaag ctccaaacca agacgctgca gtacgagttg tatcaggact gcctgaagac
     1081 catttcccgt atacttttac agctcctata aatccaacaa ttgcacaaac cacataaaat
     1141 aaatttgtga tcggttctat gtatgattta atttcttgat ttgcctgctc tagcgcagct
     1201 actccgttac cttgtgctaa tatatcgtag acattgatac acaaaagcac tgtactaaaa
     1261 acgatttttt ttggtgtttt cataaaaata aagatggggt ttattttaaa aatgaaattt
     1321 ttttatgcgc tcttaaattt gttttcgcgc ttaatttcta gattaacttg tttttgttta
     1381 gataaatatt taaagtataa atacacaaat aaattgtaca gcacaccaag tattgtaatg
     1441 gtgcacaaaa actccgtcca tgatattagt gataaactaa acatggacga ttagggtttt
     1501 tgtaataaat aaaaattgta acttgatatt agcaaaatct cttttgcaat tcaatttgac
     1561 tatataaaac ataaattaaa aattaagatg ttgggtgtcg ccttacaaac gaattagtat
     1621 catttgcttt gcgggtgcaa ataaaagcaa aatattttta actgcaaacg tttaagtata
     1681 acatttaaca tttttttata acattttttt taccacatta ttaaaaaata tattatctag
     1741 taaatattaa ttatattagt agaaataata ccttatcaac taaatttttt gcctaaatct
     1801 ataatttgca gaaaaacact aactttggca atcaatactt atacttactt ctatgttgaa
     1861 ctacatttgg gcaggattct ttatcatcgg ctttatagtt gcaatatgcc gcgccatatt
     1921 tttgcaagac ctcggagttt ttgagcgaat aataacagga acattcgata tgtcaatatt
     1981 tgcagtaatg gatatcgccc taccccttgc aggcgtaatg acactatggc taggcttaat
     2041 gaacattgga gaaaaagccg gagcaataag atttatgtca cgaattgtag gaccattctt
     2101 ttcaaaacta tttcctgaaa tacccaaaga ccaccctgcc aacggtcaac tactaatgaa
     2161 cttttcggca aacatgttag gattagacaa tgcagcaacc ccactcggac taaaagccat
     2221 gcaaagttta caagacctaa atccaaaaaa agagacagct tcgaacgccc aaataatgtt
     2281 tttagcactt aacacttcag gactcacgat tattcctatt gcaattttag cacaacgcgc
     2341 catactcgga tctgcaaatc cgacagatat ttttatcccc ctcttaatct caacatacat
     2401 atcaacatta actgctattt tgttcgttgg aatcaagcaa aaactcaatt taataaacaa
     2461 aacagttata ggctggctaa gtggtataac aatagctatc ggacttcttt gttggtacgt
     2521 gacgacactg aataaggaag aaatgatgac attttcaagt atcttcagca actccttgct
     2581 acttataatt atagtggctt ttatcagtgg agctctatac aaaaaagtaa acatatttga
     2641 gacttttatt gacggagcaa agtccggctt tgaaacatct gtgaagatta tgccttatat
     2701 ggtaggaatg ctcgttggaa tcgccgtttt ccgcgcatca ggagcattag accttattgt
     2761 tttaggaata caaaaaattg tgctgctatt ttcagatcat acagaatttg ttaatgcact
     2821 cccaaccgca tttatgaaac cgctaagtgg aagtggcgca aaagcaatga tggtagaatg
     2881 tatgagaact tacggacctg attctttcgt tggaagactt tcatgcctat tccaaggcgc
     2941 cgcagataca actttttata ttgttgcatt atacttcgga tctgtaggta ttaagaaaac
     3001 aagatatgca attggagccg gtttaattgc agatcttgca ggagttattg cagcaattgc
     3061 ctccgcatac ctcttctttg gataagcgat taaatcgtaa taaacgattt ttattaatag
     3121 tctaaattgt cttttttagt acctttgcag ttaaattctt tattatcaaa ccagaacatt
     3181 atgattagtt atcaaacaga aggtgttgag aaacctaaaa tatgcacaag acgcacaagt
     3241 aagtggataa aagaaattgc aaattcacat aacaaaaaga tcggtgaaat tgcgtatatt
     3301 ttttgttctg acgaaaaaat actacagata aataaagaat atctaaaaca cgattattat
     3361 acagatatta ttacttttga ttacacagaa gacgataaaa tatcgggaga tatttttata
     3421 tcacttgaaa cagtaaaaag caattcagaa caatttggaa ctgattatta tgacgaacta
     3481 tcaagagtta taatacatgg aattcttcat ttatgcggat ttaaagacaa agaaccaagt
     3541 gcaaaagcaa aaatgactga tgaagaaaat aaggcacttt cagcaatgag cgaaaagggt
     3601 ataaaaaagt aagacagccg ataaaaaaat aacagatgaa ttttgattac gatgtgattg
     3661 tagtaggtgc aggacacgca ggatgcgagg ccgctagagc tgccgctaag cttggttcgc
     3721 gcactctatt gatcacaatg gacatggcaa aaattgcaca aatgagttgt aatcctgcaa
     3781 taggaggtat tgctaaaggt cagatagtta gagagataga tgcacttggc ggaggaatag
     3841 gctatgtaac agatcgttct tctttacagt tcaggatgtt gaatctttca aaaggaccgg
     3901 caatgtggag tccaagatcg caatgcgact ttacaaagtt tatcctaaca tggagatact
     3961 tactagagca gactgagaac ttaaatatat ggcaagacac tgttagtcag ctaattatat
     4021 ctaacaataa agtagtagga ctaaaaacca gcttaggggc agagtttaaa gctaaaagtg
     4081 ttgtgctgac aaatggtacg tttttaaatg gtttaattca tgttggtaaa gtacaagtga
     4141 aaggtggaag aatgtcagaa cccgcaagta ccggccttac agaacaactc acttctattg
     4201 gatttacaag ccagcgtatg aaaacaggaa cacctgtaag aatagacata cgcagtgttg
     4261 atttaagcct aactgaaatt caaaatggag attgcgattt tcataagttt tcttatctag
     4321 actacgaacc aagaaactta aagcaattac catgttacct tttaaacaca aacgaagagg
     4381 tacataatgt tttgagaagt ggtataaaag actcccctct ttataacgga caaatcaaaa
     4441 gtataggacc aagatattgt cccagcattg aaacaaaaat tattactttc cctgacaaac
     4501 aaacacatcc tttgtttttg gaaccggaga gtgaagaaag cacagaatat tatcttaatg
     4561 gattttcttc ttcgcttcct atggatatac aaataaacgc tcttcaagca attcctgcat
     4621 ttaagaatat tcatattttt agaccgggat atgcaataga atatgactat tttgaaccga
     4681 cacaactaac acactccttg gaaaccaagc aaattcaaaa tctatttttt gcaggtcaaa
     4741 taaatggcac tacaggatat gaagaagctg ccggtcaagg tttgattgca ggtatcaatg
     4801 cacatcaaaa atgccatgga ggatctgaat ttacactgca aagaaacgaa gcatatatcg
     4861 gcgtattaat tgatgattta gtaacaaaag gagttgatga accatatcga atgtttacat
     4921 ctagagccga atatagaatt ctattgagac aagatgatgc tgatgcacga ctaactacaa
     4981 agtcacacaa aattggattg gcagataaca ctcgttataa actttggaag gaaaaagaaa
     5041 aacaatgtgc gattctaaaa gattttctag aaaccacaac tgctagtccg gctgagatta
     5101 atcctctttt agaaagactt ggaaattctc caataaaaca aggagccaaa tacaaagata
     5161 tactcttgcg ccctgatttg tctattacaa aaatggcaga agaacttcct ttgctcaaat
     5221 taaaaattga cgaaattctg aacagaaaag aagaaattac agaagctgta gaaatctctg
     5281 ttaaatatgc cggatatatt agtagagaaa aattgattgc tgacaaatta caaagactag
     5341 aagaaataaa aataaaaggc aaattcgaat actcaagtat tcaggcaatt tcaatagaag
     5401 ctaggcaaaa gctaacaaaa gcagatcccg aaacaattgc acaagcagct cgtataccag
     5461 gcatatcgcc aagtgatata aatattttgt taaccttaat gggtagataa tcatgttcca
     5521 cgtggaacaa ttttagattc gttccacgtg gaacatgtaa aaaaaataaa atcatgacta
     5581 ttgaagaagt aaaaaaagaa atcagagatg ttccggattt tccgcaaaaa ggaattttgt
     5641 ttcgcgactt cacaactgca tttaaaaaac cggaatgcat gaaatggttt gaaaataggc
     5701 tggtagatat gtacagcaac ctaaatatta caaaagtagt aggaatagaa tctcgtggct
     5761 ttataatggc tcctattctt gcaaataaaa ttaatgcagg ttttgttcct atccgcaaaa
     5821 aaggcaaact tccggcaaac gttattgaaa taagttacgc aaaagaatat ggagtcgaca
     5881 caatacaaat acacgaagat gccctatcat cagacgatat tgttttgctg cacgatgacc
     5941 tattagcaac cggtggaact atggaagcag cttacgagtt aattaaaaag tttggagtca
     6001 aaaaagtata tataaatttt ttagcagaac tagatggctt aaatggtaga agactatttg
     6061 atgctaatgc acaaattgat tcgcttattc attattaaat ataaatgtga gcacacgtct
     6121 tgaacacata aaacaaattg tatcttcatt acctgaaaca cccggtgttt accaatacta
     6181 caatgaagac ggtttgataa tatatgtagg caaggcgaag aatttaaaac gtagggtttc
     6241 ttcttacttt aacaaaacac aaacttctgc aaaagtaaac gtacttgtaa aaaaaatata
     6301 cgacttaaag tatattgtgg taaacacaga agcagacgct ttacttttag agaacaatct
     6361 cataaaaaaa tatcaacctc gatataatat tttacttaaa gatggtaaaa catatccttg
     6421 gttgtgtatc acaaaagaag aatttcctcg cgtttttaaa tcaagacagg tttttcgcgg
     6481 agctgaatac tttggtccat atagttcagt ttgggtttta gatacactac tcgaactcat
     6541 taaaaaaatt tatcctatac gcacttgtag acttccgctt aaagaagata gtatcgctgc
     6601 aggtaaatat agtgtttgct tgcagtatca cattcataat tgtcttggat gctgcgtagg
     6661 atatcaaaaa aaagacgact accaaaagat gatagctgaa atcaaggaaa tagccaaagg
     6721 aaatagccgc gcaataacag actatttatt aaaagagata caaaacttag ccggaaatta
     6781 tcaatttgaa aaggctcaag aattaaagct aaaatacgat gctctattac aatatcaaag
     6841 taaaacaata ataacaacca cacacgatga tgatttagac gtgtttggat acgaggaata
     6901 tgataatatt gcctacataa acatcctacg aataagcaaa ggatctatta ttcagggatt
     6961 tacaatagaa tgccataaaa aattagacga atcaaaagaa gagctattag caatagccat
     7021 attagaactt agaagtaggc tgaatagtaa ttctaaacaa ataattgtgc ctttcttacc
     7081 agaacttgaa ttaaatgata cacagataag catcccacaa aaaggcgata aaaagaaact
     7141 actagatctt tctctgcaaa atgttaagca atatcgtatt gacagactga aacaaagtga
     7201 gaaactcaat ctcgaacaaa gatcaacaca gattttaaaa gatctgcaaa acgctcttca
     7261 ccttgacaga ttgccaatgc atatagaatg ttttgataat tcaaatatct cgggcagtga
     7321 tgctgtcgca gcatgcgtcg tataccgcaa ggctaaacca tgcaaatccg attatagaaa
     7381 atacaatata aaaacagtac agggtcctga tgactatgct tcaatgcgcg aggtagtttt
     7441 tcgtagatac caaagaatga taaacgaaaa tactacacta cccgacttga taattgccga
     7501 tggcggtatc ggccaaatga atgcaataag agatatagtt gtatcccaac ttggtttgtc
     7561 aattccaata gccggccttg ccaaaaacga caagcatcaa accaatgaat tactttttgg
     7621 ttttccacca caagcaatcg gactcaaacc aaccgacaat ttatttaaat ttttatcagg
     7681 catacaagac gaagttcatc gctttgccat ttcctttcac agagaaaaaa gaagtaagac
     7741 acaaatccat tctgaacttg acgaaataaa aggaattggt gaaaaaacca aatcattact
     7801 gattttacat ttcaaaagcg taaaacgcat ccaaacggca gaaaaaatag aattagcaga
     7861 aattgttgga aacgccagag catcaataat ttatgaacat tttcatccta ttttgacggc
     7921 tccagaatta aaaatttaaa aacgattcgt aatgagggca gtaatacagc gcgtaaaaca
     7981 cgcaaaagtt gaagtagata aaaaaatagt tggggagata aaaaccggac ttttagtttt
     8041 tgttgggatt gaagacagcg ataccgaaga agacgaaaac tatcttgcac aaaaaattgt
     8101 taacctcaga attttcaatg accgacaaaa agtaatgaat ttatcagtct tagatattga
     8161 cggggaaatt ttatgcatca gccaattcac tttaatggca ataacacaaa aagggaaccg
     8221 cccttcatac attcgcgcat caaaaggatt gatagcaatg cctatttatg aagatttttg
     8281 caaaaaattg tccgaattgt tgaaaaaaga gataaaaaaa ggtatttttg gagcagatat
     8341 gcaaattatg ctacaaaatg atggtccaac tactatatgg atggactcaa aatctaaagc
     8401 gtattaacaa tcaccaagca aaagaatcaa tacaatatga aagatttcga aggattattg
     8461 aagaactacc cgtttaatgg aactgagaat aaaataaaaa gtgaggcaaa aagaattatt
     8521 gatgagcatt acgaaaacaa tgatacaagt gcctccttga aactcatatt ttcttgttta
     8581 gaaatcacat cactaaaatc aacagataac gaaaaaacaa ttgaatcact tgttgaaaaa
     8641 gtaaatcttc taaatgaaaa aataccggaa cttccaaaac tcgcaggaat ttgtgtgtat
     8701 cctgtatgtg tagaaactgt aaaaagttta cttattgaag aaatcggcat aacaagcgta
     8761 atcggcttcc cttcatccca gacattcaca gctgttaaac ttgcagaatc agcacttgct
     8821 atttcagatg gagcaacaga aatagatatg gttctacctg ttggacaatt cttaattggt
     8881 aattacgatt atattttcga cgagattcag gagataaaag cagcagtcca cgaatatact
     8941 ctaaaggtga tattggaaac agcattactt aacacatacg agaacatctt caaagcatca
     9001 ataattgcaa tggaggctgg agctgactat attaaaacaa gtacaggcaa ggaaggatct
     9061 actgatatat actctgttta cacaatgtgt ttagcaataa aagcatatta cgaaaaaaca
     9121 ggaatacaac atggcatcaa aatagcaggc ggtataaaca aaacgcaaga tgctctatta
     9181 tactacacca taataaaaga tgttttaggt gaagaatggt tgaataatga atatcttcgt
     9241 tttggtgcaa gccgactagc aaacgaatta attagcggca ttttgaaaga agagaataac
     9301 tatttttagc aactatatac ttttatgtga agtaatttgt acaagaaaac cttaaattaa
     9361 atacttatga aacggctact aactgtccta attgtttttt taagcatact ggcatcagta
     9421 agtgcaacaa ttcgtcagcc cattcagaat ttctacaaga tggactacaa gttaggtgca
     9481 cagaactggg gtgtatctca acaacgtaat ggcttgatgt attttgccaa tatgaatgga
     9541 ctgttgtctt atgacggaat aagatggact tcatctcaat ttccgggttc aggcaataat
     9601 gtcagatcat taaaaattac tccggcaggc gatatttacg ttggcgcata taacgaattc
     9661 ggagttttta aagctcaaag aaatggctta tcagaatacg aatcattatc agctctccta
     9721 ccggagaaag acctgacgtt cggtgaaata tggcacatta gtatacttaa caacagtatt
     9781 atttttcaag gaacaaatca aattttcttc tatgaaaatg gacaaataac taaattggat
     9841 actcctgcaa aaatcaaatt ctcagcagtt tttaataacg cactttatct aacatctgac
     9901 acagatggcg tttttgtttt aattggtaca agtttcattt cacttcctaa ttgcgaggaa
     9961 ttacagaata aaagcgtttg cgccattcta ccaatggaag acaatgctat cgcattcatc
    10021 accgaaagtc atggaatata cgattaccaa aatggtgaaa tgaagaaatc tacacttcct
    10081 attaacgaca ttttgattaa atatcaagca ttttgcgcag aaattaatgg tcaaacactt
    10141 gcagttggta ctgtacaaaa tggtgttgtt gttttaaatc taaaaacaaa ggaatacaca
    10201 attatcaata tggattccgg cttacaaaac aatactgttt taagcctaga ctttgatatg
    10261 gaaggtaatt tgtggttagg actagacaag ggtattgact atgtaaaaat aaactcgcca
    10321 ttccgttcat tatttgcaca aaataatccc tatggtagtg gctattgttc tttaattgtt
    10381 ggatcagatt tatatttagg cacaaaccaa ggattgtttg ttacaaagta tgctaatagt
    10441 gaaataggag ccattgaaac tattgatgga actcaaggtc aggtatatgc aatcaaacag
    10501 attgataatc atatttattg ctgccatcac aaaggactat ttgagataaa tggtaaatat
    10561 tcgcgcaaaa ttgcaaacat tgaaggtgtt tggactatac aaaaactgcg tagtaatcct
    10621 aattatttga tagcaggcca ctataatggt ttctttttga tgaaaaaaca agatggattt
    10681 tgggttttct cacataatat tgaaggtttt aatgaatctt cccgcttatt tgaacaagat
    10741 gaagccggtg atatttggat gagtcatggt ttaaaaggag tttacagaat acatctgtct
    10801 aaggatttaa aaaaagccga aagtgtaaaa ttctatggca ctgaaaaagg attcccaaca
    10861 aatgaacata tttccgtttt cagtgtgaac ggagatttga tatttgctgc agaagacggc
    10921 ttacatagat acaactctgc aacagacttc atggatgtta atactcaact tactaatagt
    10981 ttattaggag gaggacaata ttattcttta aaagaatacc aagatgacag attatggttc
    11041 gttaaatttg atcagattgg tcttgccata aaacaaaaag atgatgtgta tacatgtaat
    11101 cccaataaat gtttttctat accggaagaa ctattgtacg aatattttga cctaaatgct
    11161 ctagactcag aaaatatact tgtatctaat gaagagggat ttacattggt taatctggaa
    11221 atgttggata aaacaaattt gtttgatgtc tttattcgcc aaatttactc aacaaaaagt
    11281 gagaaaaaca ttactagtca atatcttaaa acaactgaag agtttgcgcc aattaaaata
    11341 ccatatagcg acaactcatt aagatttacg tttggtacgg ttagtttctc atcaaataaa
    11401 aacaatcaaa ccctctactc tactcgtctg ctcaattacg aagataattg gtcaaaacca
    11461 aacttatctt caagtagaga atacactaac ttgcacgaag gcacttacac attccaagta
    11521 aagacattta atcgtaattc agaaagttca tttacagaat atacattcac gatatctcca
    11581 ccatggtatc gctctttctg ggcatatgcc atatatttct tacttttaat tggtttattg
    11641 ttgctgctac gcaaatatat ccaatacaaa gaaagcttgc ttgaaaatga aaacaagaaa
    11701 caaatggagg ctcaaaaaaa attgcatgaa gaagagtctg ccgaaaaaga gaaagaaatt
    11761 attgctctta aaaacgaaca actttcgagt gaacttgttt ataaaagcaa cgaattagcc
    11821 agttcaacaa tgaacttaat ccgtaaaaat gaagttctaa ttgacataaa agatgaaatc
    11881 agcaagattg ccggcggctt atcaaatagt gaagaaggaa agtctatttc aaaaaaacta
    11941 actcgattag tacattccat taactcaaac atagaacacg acgacgattg gatgaagttt
    12001 gaacaaaact ttgacaatat tcaccaagac tttatgaagc gtttgagtga acaatacaaa
    12061 ggacttaccg taagcgataa gaaactatgt gcatacctaa aaatgaatct tgtaagtaaa
    12121 gatattgcac ctcttttaaa tatctcagtt agaggcgtag aaattggtcg ttatagatta
    12181 cgcaaaaaac ttaatttaga tagggacaca aatcttacag aattcttaca gaacttttag
    12241 aaaataaaaa ccacatggaa acaaaaaatc catgtggttt ttatttaata tttagtattt
    12301 ctaaacgttg aaacggaaat gcattatatc gccatcttgc acttcatagt ccttcccttc
    12361 cacatatatc tttccggcct ctttacaggc actttctgac cccaatgata tataatcctc
    12421 atacttgata acctcggccc gtataaagcc tttctcaaag tctgtgtgta ttacacctgc
    12481 acattgtgga gccttccagc cttttctaaa tgtccaagca cgaacttcag gctcgcctgc
    12541 cgtcaaatat gtttgtaaat ccaacaattt gtatgcagct ttaattaatc gagaaactcc
    12601 ggattctttc aaacctactt cttctaaaaa catttgtcgc tcttcataag tttcaagttc
    12661 tgcaatatca gcttccgttg cagctgcaac aattaatatc tctgcatttt catcctttaa
    12721 agacgctttc aattcatcaa cgtagtgatt gccgttaaca gcactagcct catcaacatt
    12781 gcaaacataa agaatcggct tatctgtaag caaacaaaag tctttagtca atttgcgctc
    12841 ttccttatct aaaacaagag ttcttgcaga cttcccttgt aacagaactt ctttatattt
    12901 aaataagatc tcaagtatcg tctttgccgt cttatcaccc ccactctgtg cctgtttttg
    12961 aactttaata attctgcttt caactgtttc taaatccttt aattgcaatt ctgtgtctat
    13021 gatttctttg tctctaacag gatttacact tccgtcaaca tgggttacat tggcatcatc
    13081 aaagcatctt aaaacatgaa ttattgcatc tgtttctcta atgttagcca agaatttatt
    13141 tcccaagcct tcgcccttgc tagctccttt taccagacct gcaatatcaa caacttcaac
    13201 tgttgttgga actatacgtt gtggatgtat aagttctgct aatgcagtta aacgctcatc
    13261 aggaactgta attacgccta cattgggctc tatagtacaa aaaggaaaat tagcagcctg
    13321 agctttggca ttagacaaac aattgaataa tgtagatttt cctacgttag gtaatccaac
    13381 aattccacat tgtaaagaca tcttgtttat ttttaggatt taaggctgca aaagtactaa
    13441 aaaattgatt aattttgccg aaactttaac gagcaatgaa aaattcttcc aaaaacatac
    13501 caatcatcat ctcaattatt ctgagtattg ctgtgattgc tttaggagtt ttgtttttcc
    13561 tacagcataa ggacttacaa gaaaaaactc aggaactaag ttctgtgaca gccgaaatgg
    13621 agtttgaaaa acagcaatct gtagaagaat tcgaacaaat agctcatgaa tacgaaaact
    13681 tttacattaa cacaagtaat gactctttac ttaaattaat tgataatgaa aagcaaaaag
    13741 tacagcaatt attgcaagaa ttaaaaacag taaaagcaac taatacacgt cgtattggtg
    13801 aattgaaaaa agaattaagc actgttcgag gtgttcttaa atcttatgtt gaaaaagttg
    13861 attctcttaa cgctttaaat caaaacttac aaaaagaaaa ttatcaggtt agacaacaat
    13921 acgaacaaac ggccaaggaa ttggaacaaa aagctgctga agcagcagag ttagataaaa
    13981 aagtcagtat ggcagccata ttagaggcta gtaatatcaa tataagtctg ctaaacgaaa
    14041 agggccaacc aacaaaaagc cttagaaaaa ctgcaagttt tgaaatttgc tttgatattc
    14101 tcagaaacat cacagcagaa cggggtaaaa aaatggttta tatgcgtatt acagattata
    14161 aagaagagtt gatgacttta cccgggcaaa acacctttaa tttcgaaggt aatcagatag
    14221 aattttccgg caagaaagag attgaatatg gtggagaagc cgttcatgtt tgtatatatt
    14281 atcctattca agaaaaactt actaccggta catacagcat tgctattttt atggaaggca
    14341 atttaatagg atcaaaaagt tttgctatta aatagaaatg gccggaatct acatacacgt
    14401 ccctttttgt acacaacgct gctcttactg tgatttcttt tctaccatcc gattagagga
    14461 aatggattca tatactgatt ctgtttgttt agagattata caaagaaaag actatatcaa
    14521 agacaaacat attaatacaa tatattttgg tggaggaacc ccttctcttc tttcaaaaga
    14581 aaatttaaca aaaatatttg atactctata taaaaacttc gattttagca accttaaaga
    14641 agttacttta gaatgcaacc ccgatgatat tcggaaggat tttttctgta aaattagtga
    14701 cacacctatc aatcgtctaa gcattggcat ccaatctttt caggatgatg aattagtaat
    14761 gctaaatagg cgacacaatg ctgaacaagc aaaaaaagcc gtaaaaatgg ctcaagacaa
    14821 ttattttaac aatatcagta ttgaccttat gtatggactt cctcaacaaa aaattgatct
    14881 ttggaataag acaatacaag aagctctgtc actaaatgtt caacatatat cagcatatca
    14941 tttaagttat gaagtaggta caaaaatgta ttcaatgaga aataaagctg ttgatgaaga
    15001 gactagttta gaattatttc aatcgttgag acattcttta attggtggcg gttttgagca
    15061 ctatgaaata tctaattttg ctaaaacaga atttcgatct atacataatt cttcttattg
    15121 gaaaaatgtc gaatacttag gattgggtgc cggtgcacac tcttacaacg ggtgcaattc
    15181 aagacaatgg aacctatcta atctagataa atatcaacaa gcggtaaaaa acaatggaat
    15241 atattatgaa caagaaattt taaatgatga cgatatttat aatgaattga taataaaatc
    15301 attacgtacc tgtgaaggca taaacataaa taatatcgac aataaatatt tagatcattg
    15361 tattaaaaat tctaacttat acacaaaaga aggcctactc tgtaaacaag ataattttat
    15421 taagctaaac gaaaatggta tttttgtatc cgatagaatt atacgcgatt tgatgatatg
    15481 aatattatta aagcatattg gaaaacatca ttgataagca ttattattat atacttatca
    15541 atgattaaaa tgccttcttc tatagaaatt cctaagattc cttattacga taaatgggtc
    15601 catcttatca tgtacgccct tttgtgtaca attgttttat ttgatagtat taaaaactta
    15661 caccgcagac catatattcc tgcggctgtt tattcaatta tacttggtgg tatattagaa
    15721 ttattacaaa tactatttcc accaagaagc gcgagttttg ccgatttttt agcaaattta
    15781 ctcggctgta gtttaagcct aatcatcttt ttcttaataa aatttattca tggaagaaca
    15841 actaattcaa aaaaccattg cattaactct gattgatgga attggtacac aaaacacaaa
    15901 aaaactaatt gattactttg agaacgcaga taacgtgtta agacaatcag cacgttcatt
    15961 ggcttcttta tccggaatag gacaaagcaa ggcagaaagt attgtaagtc aatttaatga
    16021 tgtattaaaa aaagctgaag tagagcttaa atatatctac gacaacagga ttaatcttca
    16081 tttttacaaa gattctaatt ttccaaagaa actgcttgaa tgctccgatt gccctgtttt
    16141 gttatactct aaaggacatt ttgattttga aaatggtaaa tatatatcta ttgtaggtac
    16201 tcgcaatgcc accgaatatg gaaaaaaatt atgccaagat tttgttcgtg atgtttctat
    16261 aagacaagct gatactacaa ttataagtgg attagcttat ggtatagaca tttgcgctca
    16321 tctttctgca atagaaaacg atttaccaac cattgcaatt atggcaggtg gttttgcaca
    16381 tatttatccg ggtgcacaca aacatgttgc tgaattaatg gtagaaaaag ggggtatcat
    16441 aaccgaaggt atatctagta cctttccgga acgacaaaat tttttaaaac gcaatcgcat
    16501 tattgcaggt ttagcagatg ctgtggttgt agtagaaagt gcagctaaag gcggtgcatt
    16561 agttaccgca gatattgctc attcgtacaa tagagatatt tttacttttc cgggtagggt
    16621 taatgatact tggtctaaag gttgtaatca attaattaaa caacaaaaag cggccttaat
    16681 tgaatctttt agtgatttag aatatcttat gtcgtgggat aaaaacaaaa taaaatatat
    16741 tcaaacatct ttatttaatg atttaacaga aaaagaacag gctgtttact ctattataaa
    16801 gaaggaaaaa aagttgcaca aagacttatt aaatcagttt tttgaaaatg aaacttcatt
    16861 acaaagtata ttgctttcac ttgagtgtaa ggatattata aaatgtttac ctggtaattt
    16921 tttcattcct ttataattat ggagggtata taaaaaacaa aaggcactca cgcttgagta
    16981 cctatttgct aaccttaaaa tcaaatacta tgaaaaaatc acaatgcaaa tgtaagggta
    17041 attatatgct gtgcaacata taattctttt tttttacaaa tttggcaaca aaaagaacta
    17101 gaattcttgt taatattaca aattagttac aatactattt tttatgtcaa tacagtgtta
    17161 caaaaattgt ttataacttg aacattattt ttcgaaaata aattttgatt attgaaatta
    17221 gattgtctat aaaaactatt ttttaaaata aaaatatata aaaatgaatt tctatttaat
    17281 aataaacagt caaatgaact tattttcaga gaatctaata aaaaagttta ctacatttgc
    17341 acttatcaac agtatgttag taaacttctt agtattcaaa taaacaataa gttaagctgt
    17401 tcattatttg ttattagtat gttagcttaa aacaaattat tttaaaaaca agcttatttg
    17461 atttatttta ttgacagtat taacacccta ttattattat catttatttt ttaaaaacta
    17521 aaaacaataa tatatatagt aaacaataaa aactcatgat atggatttta taaaagaaat
    17581 aaataaacta agaaaagaaa aaaatgcagt aataatggct cattattacc aatccggtga
    17641 tattcaagat atagctgata atgtaggaga tagtttgcaa ttagctcaat gggcagctaa
    17701 aacagatgca gatataatag ttttatgtgg tgttcatttt atgggcgaaa cagcaaaaat
    17761 tttatctcct aataaaaaag tattaatacc tgatattgag gcaggatgtt cattagcaga
    17821 tagttgttct gctgatgaat ttgaaaaatt tataaagaag catcctaacc atagagttat
    17881 atcttatgta aatactacag cagcagtgaa agctctttca gatgtattag taacttcaac
    17941 aaatacaaga aatatagtag aatcatttaa taaggatgaa aaaataattt ttggtcctga
    18001 taaaaactta ggtaattata ttaatagtgt aacaggcaga aatatggtat tatggaacgg
    18061 agcatgccat gtacacgaaa aattttcagt tgaaaaaatt gctgtattaa aaaaagaata
    18121 cccgaatgca ttagtattag ctcatcccga atgtaaagca actgttttgg caatggcaga
    18181 ttttgtgggt tctactgctg ctatattaaa ttttgcggga aaatcagaaa agaaaaattt
    18241 tatagtagca acagaaagcg gaattttaca taaaatgaaa ttatcatatc ctaataaaca
    18301 atttataccg gctcctccgg atgatgaaat atgtgcttgt aatgaatgta gttacatgcg
    18361 tttaaacact atggaaaaat tatataaatg tttaagagat gaaaatcctg aaattaaaat
    18421 agatcccgaa atacaagaga aagctgttaa acctattttg agaatgttgg aactatcaaa
    18481 ataaattaaa atttaaagga aactaaaagc cttaaatcta tttttttatt tcctataatt
    18541 tcttcatttg cactagctat agtttctcta tctgtataaa aatttaatgc agatttaata
    18601 tatatagacc attgcgcaga tggcatatat ttaagcatta agcaattccg aaatccttga
    18661 ccagaatatg aaggagagga atatgcaaat aaaacgtctt tttcgtagca gtatattcta
    18721 ttttcatata atggaacatc aaaaaatgcg catttgaggc ttatttgtaa gggaaatttt
    18781 atgaaaatat aattgaaatc catatataaa agccaagcat aatcaatatt tgaattcttt
    18841 atatatatat ttgactgtgc gcctgtttga aaagtaaaaa aatcactaag ttttgcttga
    18901 tatgtaaatt tcaacgaatg ttttctataa ttagtaaccg tctgtgtatg cgataaatta
    18961 tcatcattat cgtttacact catttgttta caattatatc ttagtaaaat ataatgatta
    19021 ttatttggcg tgtaaattaa ctgacagaaa gttttaaagc cttctgaagg ttgttttatt
    19081 tgatatttaa aagatttgaa agcaaaaaca tctgcaaaag ccgaaatctt ccaataccta
    19141 aaaggcctga tttcaaaacc aattaataat ccttgctcat cattcaagcc ggattctgaa
    19201 tatgcgcatc cgtatgtaga attgtagttc aaatcataat acctatacag tgcagaaaaa
    19261 ctcagttttt cattcataag aaaattaata tgatttatag tagcaaaacc acctttttca
    19321 ttgactgcaa attctccggc tatataaaag cgcttaattt tggctctata atctaatccc
    19381 acattaattt ttttagaaag aaattcgtaa caaaaagagc tcccaatttt aaaaaattta
    19441 tgtctgtaaa gcgcattaaa tcctaaggag taagagttgt tggtgttttt tttgataaaa
    19501 gatgaaattg tacgatgata attttctgaa ttatactgag tatgagatgc aaaggcagat
    19561 aattgtattt tgttgaattg caaagtagta gcaaaacctc taagaaaacc ttgttctgaa
    19621 acagaaaaga aaggttgaaa cccatcttgt ctttgacata aagcagctaa atcggttgag
    19681 tttatatgat acgaatttga tttataaatt aacccctgtc caaaattcgc tttaaaatca
    19741 ccaataatca ttaatttaca atgtttaatg tctttgatag aaatatggcc tgaataaaaa
    19801 tcaaatcctt tattgtattt ccaatcaaaa ggttctcctg catctttgtc catactaact
    19861 gcaatacttg cgttcttatg tgtaaaggca tatttaaaat aggaagccca agcttttcct
    19921 aaataagccc catttgtata tccttttttt tgtggaaaac cgtaatcagc tctaatagta
    19981 attctatgct ttgcaaactt gaaaatattt tttaaggaaa ccttttctaa atcggctttg
    20041 gcatatacta ttaagaaagg taaacaatta gaaatggttt catcatcaaa attttttatt
    20101 aatgatagtt catatatact ttgaaaatca ccatagtcaa atctatactc taaaagattt
    20161 tctatttgcg tttcgtttaa aaaagggaat tgttctaggt attctctgtt tatttcattt
    20221 agattcatgg gattctcagc agccttgtac aagaaatcag caatactatt attatcatca
    20281 gcgttatcat tatcatgtgt attttctgaa aactcagaaa caatatcttc aatacttttc
    20341 gtagtgggtg atgaaaatac tttggtacac atgaaccata aaattagtat ataaaaaagt
    20401 atttttctca tcttttttta agcctgtagt ataaaccaaa tgcagaagat atgcctaatt
    20461 ttgtatcgta gctgatacct aaatcgcaac caaaggattt ataatttaat ccgataccaa
    20521 tggtagatac taagtttttt tgaccacaca aaccggctct tatagtgaca aatttgatag
    20581 gttcgtattc tataccgcca ccaataagaa taggggagct taattcttta gataattcaa
    20641 atttaagaag aatgtttttt tcaaatttat aacttactcc gattttaaaa atagaaggaa
    20701 tacggatttt caaattggta caatgaacag gagaaaagga taaattatag gcatgtacgc
    20761 taatattcaa agagttagta ggaaatacta tcatacccag ctgaactatt gcagtgcttg
    20821 caacaccatc atcttttgat aaaaaaatgc cgatataatc taattctaat ccgatagaaa
    20881 agtaaggttt aaaccatttt gcaaaagcta tccctgcgga tatttctcca tattcggaaa
    20941 aaccataata atctattgat gcgcaccatg ttccaaattt acaaggataa actaatccta
    21001 attgtcctcg tccaagggag ggtagaaaaa aagagttagt gtatgtagtt tgtattatag
    21061 gaaattcaca caatgctgca acagcctgat tgtaaggtac atagttctga ttaatatttg
    21121 cagaggcatt ggttttacca attgcaagac caacggggtc tattctattt cctaattcca
    21181 ttgcgtaaga gcaatgtgga aaaataatta aaagcaggaa aaatgagcgt ttcatgcgta
    21241 agaaaaatta aaatggtgca caaagataca tataatgaat tatttttgtc aagagaataa
    21301 atgaaaaata aattaatcat attatttatt acagcaacaa tattaagttg tgggtctgta
    21361 tgtgcgcaga attactatta cagtgaatct ttgcatggct atatattgcc cattgcagta
    21421 tatggaacag atacaatacc atgggtagaa ttgcgtgaag tgcctattta tcctcccata
    21481 aaaaacaaaa aacaagaaaa attttattgg aaaacagtaa gagacgtaag aaaggtttac
    21541 ccatatgtaa aaatagtagg aggtgaatat gctcgcatta acagcatgtt tgatactatt
    21601 caggatgtaa aaatcaggaa aaaatttatg aaaaaatatg agaaagagct acttaaaaaa
    21661 tatgaaccga tattacgaac atttacttta tcacaaggta aaatgatgat aaaactaatt
    21721 gatagagaaa caaataaaac ttcatatacc ttgattaaag agttcagagg gggctttgtg
    21781 gcttggtggt ggcaactttt tgccaaattg gtaggagccg atttgaaaga agattatgat
    21841 ttctcaaaaa aagaaaaaga tcgcatcata gaaagagtaa tcacattata tgaagccggt
    21901 ttgttgtaaa ccggcaaatt aactatcttt gcagcactta taatattagg tgcaatggca
    21961 aaagaattaa ctccacgtag cgagaattat tcgcaatggt acaatgattt agtagtaaaa
    22021 gcagacttag ccgaaaattc ggcagtcaga ggctgtatgg taattaaacc ttatggctac
    22081 gcaatatggg aaaagattca acgtcagttg gatcaaatgt ttaaggacac gggt
//
LOCUS       DAOPID010000005        20070 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_2893_length_20070_cov_5.292581, whole genome shotgun sequence.
ACCESSION   DAOPID010000005 DAOPID010000000
VERSION     DAOPID010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 20070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20070
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2893_length_20070_cov_5.292581"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PK641_00525"
     CDS             complement(<1..208)
                     /locus_tag="PK641_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HPG54873.1"
                     /translation="MIESILRNTVLFIVLVFLQVVLFDNIDYFGFISPYIYVIFILSL
                     PVGINKNWAMILSFILGMLIDMFSN"
     gene            complement(208..1041)
                     /gene="mreC"
                     /locus_tag="PK641_00530"
     CDS             complement(208..1041)
                     /gene="mreC"
                     /locus_tag="PK641_00530"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HPG54874.1"
                     /translation="MGNLIRFLIKFSSLLLFLCLEIVAIILMVNQNNYHRSVFLSTSN
                     VVSGNVFNTVDMVTGYFNLRHNNDLLSEQNTALKNQLLEMSNRMSTYQDTVQDSIYID
                     ADKDYHYIRAKVIGNTVNKFQNYITLNKGERDGVKPDMGVVCSEGIVGVVQTVSKNFC
                     VVLSLLNPQSKISCRLDSCKNFGSLVWDGVNPMYASLEEIPRHVPVRLNETLSTSGFS
                     SIFPEGIPAGKVVEKELTESDNFYSIKVELSTDFRRLSYVDIIVFANSTELQELERSI
                     K"
     gene            complement(1099..2124)
                     /locus_tag="PK641_00535"
     CDS             complement(1099..2124)
                     /locus_tag="PK641_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPG54875.1"
                     /translation="MGLFSFTQEIAMDLGTANTIIIQNGKIVLDEPSIVALDKRTDKL
                     VAIGKQARQMQGKEHDHIRTVRPLRDGVIADFYAAEMMIRGMIKMISSKNSFFSPSLK
                     MVVGIPSGITEVEMRAVRDSSEHAGGRDIYMVFEPMASAMGIGLDVEAPEGNMIVDIG
                     GGTTEIAVISLGGLVCNRSIRIAGDDLTADIQEYMGRMHNIKVGERTAELIKINVGSA
                     LTELSDEEAPEDYIVHGPNRMTALPMEVPVSYQEIAHCLDKSISKMETAILSALEQTP
                     PELYADIVRNGIFLSGGGALLRGLSKRLYDKMNIQFHIADDPLHAVARGTGLALKNVD
                     NYSFLMR"
     gene            complement(2166..3686)
                     /gene="purH"
                     /locus_tag="PK641_00540"
     CDS             complement(2166..3686)
                     /gene="purH"
                     /locus_tag="PK641_00540"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HPG54876.1"
                     /translation="MEQKQIKTALVSVYHKENLDKIILKLNSEGVKLLSTGGTQSFIE
                     SLGVPCQAVENLTGYPSILGGRVKTLHPKVFGGILGRRNNVEDLEQMSKYEIPEIDLV
                     IVDLYPFEATVASGASEKDIIEKIDIGGISLIRAAAKNFADVIIVASQNQYASLIDLL
                     DENGANSTLEQRKFFAKEAFGVSSLYDSAIFNYFDGDAQSSFRYAKDDIKSLRYGENP
                     HQKGFFYGNFDEMFEQLQGKEISYNNLLDIDAAVNLIADFDETTFAILKHNNACGIAS
                     RNTLLEAWTDALASDPVSAFGGVLITNKPIDKTTSEEINKIFFEVIIAPDYDMDALEI
                     LSQKKNRIILIQKETKIPTKQFRSVLNGLLSQDRDTSVETEKDFKQVTTKQPSKAEIE
                     DMIFANKVVKHSKSNAIVLAKNKQLCASGIGQTSRVDSLRQAIEKAGTFNMDLHGAVM
                     ASDAFFPFPDCVEIAYNAGITAVVQPGGSIKDQLTIDFCENNGIAMVTTGIRHFKH"
     gene            complement(3797..4288)
                     /locus_tag="PK641_00545"
     CDS             complement(3797..4288)
                     /locus_tag="PK641_00545"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007766775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HPG54877.1"
                     /translation="MPNLVLIVAMDEMRGIGYKGQLLCYLPNDLKHFKQLTTGHTVIM
                     GRKTYDSLPNGALPNRRNIVISRQKDLKIAKCEVVDSLDSAFALCSSDEDVFVIGGAS
                     IYSQALAHANRLELTIIDGRFESDVFFPNIDTQIWSFEKEEKHPADEKHKYSYSFVTY
                     LRK"
     gene            complement(4424..5218)
                     /locus_tag="PK641_00550"
     CDS             complement(4424..5218)
                     /locus_tag="PK641_00550"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HPG54878.1"
                     /translation="MKQYLDLLNRVMNEGVRKEDRTGTGTISVFGHQMRFNMADGFPT
                     LTTKKLHLKSIIYELLWFLNGDTNVKYLQDNGVKIWNEWADADGNLGHIYGYQWRSWP
                     DYNGGHIDQIKETVETIKNNPDSRRIIVSAWNVADIPNMNLPPCHAFFQFYVADGKLS
                     LQLYQRSADIFLGVPFNIASYSLLLLMMAQVCDLEPGDFIHTFGDAHIYLNHIDQVKL
                     QLSREPRELPTMNVNPDVKDIFGFHFEDFELVNYNPHPHIAGAVSV"
     gene            complement(5258..7255)
                     /locus_tag="PK641_00555"
     CDS             complement(5258..7255)
                     /locus_tag="PK641_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPG54879.1"
                     /translation="MMIKRILIGLLTVVLFASCSLNARITKADKKFALGEYYRAAEMY
                     RSIYPNVPTKKRVLKADVAYKMGNAYRIVENNKRAETAFKNAVKYGCKDSLLTYYYAE
                     VLRRNGKYKEAIKMYYQYEQMRPGNELAKNGIESCEKAIADWEKITAYEIIKDPTFNS
                     KYSEFCPAFASADGDVLYFNSTRVARGAKKTKTSRITGQRNNDIYSSKTNAQGKWESP
                     NPLEGEINTEFDEGTCSFSADGQELYYTLSKVVKGETLGAAIYMCKRNGGAWSEPQKL
                     QILKDSSITTAHPALSPDGAYLYFVSDMDGGFGGRDIWRVAKTGDGWDIPENLGPTIN
                     TKGEEMFPYFRADGVMYFSSDGHPGLGGLDIFAASKRPKAKFSDADEWDVVNLLKPIN
                     SNFDDFGISFAGKQDWGFFSSNRNDRKGYDHIYKFSKPVLNFSVVGVITDSKGENLGD
                     ARVRMVGNDGSIANIQTKKNGSYTASINKNTKYVLLATCRGFLNSKKILDVADLEKSQ
                     NFTMDFQLNSVSKPVKMNNIFFKFGSAELTPESSAGLDELVELLKDNPNITIEIGAHT
                     DFVGSDETNLLLSGQRAKSVVDYLTVKGIEKDRLSSKGYGESVPVVPDKALVKQYRFL
                     KVDTPLDNEFIEGLKSEEQEIANQINRRTEFKVLKTTYKMY"
     gene            complement(7252..7956)
                     /locus_tag="PK641_00560"
     CDS             complement(7252..7956)
                     /locus_tag="PK641_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPG54880.1"
                     /translation="MNEEKVKILLCEDDENLGMLLREYLQAKGYDTDLLPDGDAGYKA
                     FMKTNYDVCVFDVMMPKKDGFTLAQEIRAVNTEVPILFLTAKNLKEDIIEGFKIGGDD
                     YITKPFSMEELLFRIEAILRRIKSKKMKEKVVYKLGNFTFDSQKQQLTINGKTTKLTT
                     KESELLSLLCANANDILERNYTLKTIWIDDNYFNARSMDVYITKLRKLLKEDETVQII
                     NIHGKGYKLIVPETAE"
     gene            complement(7961..9451)
                     /locus_tag="PK641_00565"
     CDS             complement(7961..9451)
                     /locus_tag="PK641_00565"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPG54881.1"
                     /translation="MRKGTIILLAILMALTFLGLLAVQLNYFSTLIEIRQQHFDDGVK
                     RSLYQMSRTLEEEETSEYLNDALQKESERKTIIPKVDNGTFLSIDTNPSIQTVSLKSV
                     NQTNTVQELTENLQSRMKNNYARKKILLNDAAMRWLKEAPEKPIEQRVDMEHAEQLLR
                     QELLNNGIDTPFHLILLDYLGRQVYICPDFDSTCVSSNYYSQILFPNDYNPKMNLIRA
                     YFPERSQYILMSVSSLAITSIIITLMLFCTFVSTILLMFKQSKLSKSKTDFMNNMTHE
                     FKTPISSISLASQMLQDDSIIKTPEVLKSTSRIIRDETKRLSFQVEKVLQISLLENEK
                     SIMKFKDADVNKIIDGVVASFRIKVKSTGGTINTDLKAADSWATVDELHFTNVIFNLL
                     DNAVKYSKPDEPLYLMIQTWNELEKDLLCISVEDNGMGIKKEHLKKIFDKFHRVPTGN
                     VHNVKGFGLGLAYVKKMVEKHNGHIKAESELGKGTKFIITIPTLKK"
     gene            complement(9554..10714)
                     /locus_tag="PK641_00570"
     CDS             complement(9554..10714)
                     /locus_tag="PK641_00570"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HPG54882.1"
                     /translation="MNDKINLVAKTFQGLESVLAKELENIGAKNVRIERRAVLFEGTT
                     ETMYKANLHCRTALRILKPIAIFQAKDAEEIYNNVKKIAWEKIFDVKQSFLIEATVYS
                     EVFTHSRFATYKTKDAIVDYFQEKVGKRPSISVSNPDIFFNLHISHNTCTLSLDSSGD
                     ALYKRGYRVAQTEAPINEVLAAGMILLSGWNGQCDFLDPMCGSGTIPIEAAMIALNIP
                     PGVFRKNFAFEKWLDFDEELFDKLYNDDSGEKKFNHKIYASDISSKAISVADANIKNA
                     GFTKYISTQVIDFANIPQPKEKTFIITNPPYGERIGGDIDSLYGMIGTTLKHKYADTD
                     AWIISSNKRGFDQIGLKPNDKIMLQNAEIDCQYRHYHLFPGKLKTYKSANKE"
     gene            complement(10707..11162)
                     /locus_tag="PK641_00575"
     CDS             complement(10707..11162)
                     /locus_tag="PK641_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HPG54883.1"
                     /translation="MRTLYLFIAFIAFGILIVILNKFTLKHKKDVKASNNPNGEAQKM
                     QKQKQASKRVIPEGCCGAHEVCEKESLITTKKDIIYFADEELDAYKGRTSDSYTDEEI
                     DEFEDVLLTLRENETAEWLKSLQLRGIEIPEIIKDQALLMVAERRNKHE"
     gene            complement(11216..12214)
                     /locus_tag="PK641_00580"
     CDS             complement(11216..12214)
                     /locus_tag="PK641_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPG54884.1"
                     /translation="MKKIALIGASGFVGTRLIDLLEEAGSYNLKNLDLLQSKFYEKYT
                     VIADVRSLDQMNKELADANCVVLLAAQHRDDVKPHSLYYDTNVEGMKVTLQAMENNGI
                     KELIFFSSVAVYGLNKENPNENSIKDPFNHYGKSKWQAEQVLQEWYKTHNDWVISVLR
                     PTVIFGERNRGNVYNLLRQIASGRFLMVGNGMNKKSMAYVGNVVAFVKYLIENRQKGY
                     EEYNYIDKPDYNMNDLVSLVSSVLGKHIPSIHIPYFIGKSMGYCFDLLAFITRKKLPI
                     SSVRVEKFCATTEFSADKVKKTGFKAPFTLKEGLSKTLMFEFVQKDNKDGHIFESE"
     gene            complement(12211..13329)
                     /locus_tag="PK641_00585"
     CDS             complement(12211..13329)
                     /locus_tag="PK641_00585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPG54885.1"
                     /translation="MRKILHIPNFYPPHKGGIEDVCHNIIEILKKEPDVQQKVICFSG
                     NNLSITDKFDGVDIVRVGARIKISSQFLSFAYYIKLKNLIKSFKPDYIHLHCPNPLVS
                     LYVYLAIPKGTKLIIHWHSDIVDQRKLYRIVSGIERKVLDRSNKIIATSPNYIEKSLP
                     LLRVKEKVSVVPNIINKSLFEYNEEIKKKVESIKSKYQNKKIVLFVGRHVPYKGLRYL
                     LEAMPLVSSDCVVLIGGSGVLEESLKSMNKDGRVYFIGRIPDDMLPAYYYAADVFAFP
                     SITKNEAFGVALAEAMYCNTPAVTFTIDGSGVNWVNINGVTGLEVQNKDCEAYAKALD
                     YILSNDSVCKEMASNARQRVIENFVEKEIEEKIKKLYE"
     gene            complement(13326..14369)
                     /locus_tag="PK641_00590"
     CDS             complement(13326..14369)
                     /locus_tag="PK641_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPG54886.1"
                     /translation="MKIAVDCRMLGMSGIGVYLENILYYWLETNKAHSFVLIGDEKKL
                     SKLKFGENCNVVSCSSSVFSFGAMFSFPVKEVNNCDVFYSPTFGIPFGIKVPVYSTIH
                     DVVFLDVKGLVGGLSLLVRKLFYCRAIYASKKIFTVSEFSKTRIKKHFNTNKEIVVAY
                     NGIRHDLFEWKNKKRSSDLYKNYLLYVGNIKRYKGLDILLSAVKAMSDVKLIVVGKYD
                     DMRTSDKELLNESSLNENKVVFVGQKSDEELYSLISDAEALIQPSRYEGFGIPPLEAL
                     FLHTKVIISDIPVFKEVYSKLPVIFFENGNSNDLQKKIEGISNEKSPDCIIPEIYSYK
                     NTAAIILKNITIE"
     gene            complement(14366..15433)
                     /locus_tag="PK641_00595"
     CDS             complement(14366..15433)
                     /locus_tag="PK641_00595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPG54887.1"
                     /translation="MKIFFLIKSLESAGGTERVTTVIANELSREGNEVSIICLQKCVP
                     FFKLRETVNVIPLNNKESINIYKTYLPTVWRLRQLLKREKPNCIIDVCSAMSLWSLPA
                     KFALDIKLISWEHFNAHVNWNPITSPLARKLVSKFADHVVVLTETDREVYQNEYKAQR
                     VTCIVNPITIEKKQTTTLSSKKVLAVGRLEDQKGFDLLLQTWKLCNCVNLGWKLEIIG
                     SGSKESELKSMITSLNLHDSVTMREPVTDMVSEYLNASLYVLSSRFEGLPLVLIEAMS
                     MGLPIVSFDCETGPRDIVKDGENGFLVPPMDIKALAEKIDFLVSSPNLLKSFSENAVR
                     YSMRFDLAPVMEEWKLLLKDL"
     gene            complement(15456..16277)
                     /locus_tag="PK641_00600"
     CDS             complement(15456..16277)
                     /locus_tag="PK641_00600"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPG54888.1"
                     /translation="MISVCLATYNGEKYIKEQLISILSELSEDDEIIISDDHSTDKTI
                     DIIKALNDKRVKIFENPGKNGVSTNFANAIEHATGSYIFLSDQDDVWIKGKVELSLNS
                     INAAESANPSVPVLVYTDIKIVDECLNVIVESSFAQSGQNPEDAIKENVLTVSNRLMG
                     CTMAFNELTKQYILPMPENIIMHDWWIALCVAKNGIIVPIKNATLLYRQHSGNVIGST
                     IVNKPSLMSRIKLLPQKFAFNIKIYQMVKRVYKIDFFTFALRKALLHVPQMGLKK"
     gene            complement(16274..17500)
                     /locus_tag="PK641_00605"
     CDS             complement(16274..17500)
                     /locus_tag="PK641_00605"
                     /inference="COORDINATES: protein motif:HMM:NF026247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HPG54889.1"
                     /translation="MLLEMNLMLLPILILFPLSLIEFIFPNNERMNKQVYFIAFAISY
                     FIVAIKYYYGPDIAIYVNLYDSLSSPIKDLINPPKSSLDTERLFIFFFSFLKSIGCSY
                     WFCSVIITTIYFAAIYNLFTSLNKYKTFALFLIVLFDANLLFFEFRQNLTVALFLFSL
                     SAYYKRSYFVCVLLAVLSFFIHKSAVFVYMLMILLLFFQRVKVSKTIFLVASGLLLVF
                     LFLPIDQIIIWIISKTPASTNVIESVKHHLMYTKTFQIIFFVYAIVLLLLIFSVENKD
                     LNSKYAKIVALFFIVVSISYRDWFFLNRLRSYFLPVVIVYLIPILSQSKSKQIWISKL
                     AVCLIYLYSINYARGIWLSNYYSEAKLLNSTTIFSRFYKSENEIRRENMRKASYFFQK
                     EYMKNNIEEYNKSIKE"
     gene            complement(17485..18756)
                     /locus_tag="PK641_00610"
     CDS             complement(17485..18756)
                     /locus_tag="PK641_00610"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HPG54890.1"
                     /translation="MRTKELINNNKREVKDVFYLIALQGLNYVAPLIVYPYLMVTLGA
                     EKFGYIGFSMSFCTYLMLIVDFGFNLTATKRVALYKDNKSKLNEIFSATIYAKILLLA
                     ISFLILVLFSFIPQFKVYVTTMFVVFLMVVGNAFSFVWLFQGLGNIRLVSIVNTICKL
                     SIFPITFFIVKYPNDYLLAAIVLSSVYIVSSIINAIIIWHKKWVSLIRCKIVSVKQEI
                     KESYPIFLSNAATSIYTASFVVILGYFASPDEVGKYAAVDKLMRAMSLLVLMPVTQSF
                     YPKVSRLGKQDKLGAIHLSDKLLWLVIVGMLCIAGGMFFFSDTIVELLGSDYADAELL
                     FKIMAFVPLFIGIGGVNGQLKIVAVGNSENLITFKRIYIAAAAFALVLVFILVPKFDS
                     VGAAVALLLTEFLVAVGMSIWSRNKILCSWK"
     gene            complement(18769..>20070)
                     /gene="proS"
                     /locus_tag="PK641_00615"
     CDS             complement(18769..>20070)
                     /gene="proS"
                     /locus_tag="PK641_00615"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPG54891.1"
                     /translation="DTGHVNAYFPLFIPKSFLSKEADHVEGFAKECAVVTHYRLKKNP
                     DGSGVVVDPAAKLEEELIVRPTSETIIWSTYKNWIHSYRDLPILCNQWANVVRWEMRT
                     RLFLRTAEFLWQEGHTAHATKEEAEEEAVKMLNVYADFAENFMAIPVLKGVKSANERF
                     AGAVETYCIEALMQDGKALQAGTSHFLGQNFAKAFDVTFMNKDNKQELVWATSWGVST
                     RLMGALIMSHSDDDGLILPPNLAPYQVVIVPIYKSDEQLKDIDAKVDTIIANLKLLGV
                     SVKYDNADNKRPGFKFAEYELKGVPVRLAIGARDLENNTVEVFRRDTKEKETRSLDGI
                     EQYVRDLMSDIQSNIYKKALDYRSSVTREVNSYDEFKEEIEKGGFLLCHWDGTTETEE
                     KIKEETKATIRCIPLEGDQIPGKCMVTDKPSKQRVIFARSY"
BASE COUNT         6418 a         3937 c         2901 g         6814 t
ORIGIN      
        1 tattactaaa catatctata agcataccaa gaataaacga caaaatcatt gcccaattct
       61 tattgatgcc aacaggtaaa cttaatatga aaattacata tatgtatggg cttataaatc
      121 caaaataatc aatattgtca aataacacaa cttgtaagaa aacaagcaca ataaataaga
      181 cagtatttcg taagattgat tctatcatta ttttatgctc ctttccaatt cttgtaattc
      241 agtactgttt gcaaatacga taatatcaac atacgacaaa cgtctgaaat cggttgataa
      301 ttctacttta atagaataaa aattatctga ttctgtcaat tctttttcta caactttacc
      361 tgcaggaatt ccttccggaa agattgatga aaaaccacta gtactaagcg tttcgttaag
      421 acgaacaggt acgtgacgcg gaatttcctc aagactggca tacataggat taactccatc
      481 ccaaactaaa gaaccaaaat ttttacaaga atcaagacga caacttattt ttgactgcgg
      541 atttaataat gataaaacta cgcagaaatt ctttgaaacg gtttgaacaa cacccacaat
      601 accttcgcta caaacaactc ccatatcagg ttttactccg tctctttcac ctttgtttaa
      661 agttatataa ttttgaaatt tattgaccgt attgccaata accttagccc tgatataatg
      721 ataatcttta tctgcatcaa tataaattga atcttgtaca gtgtcttggt aagtcgacat
      781 cctatttgac atttccaata attgattttt taatgcagta ttttgttctg atagtaaatc
      841 gttattatga cgtaggttaa agtaacctgt taccatatca acagtattaa aaacatttcc
      901 gctaacaaca ttcgatgtgc ttaaaaacac cgaacgatgg taattgtttt gatttaccat
      961 caatattatc gcaactatct ctaaacataa gaacaatagt aacgaactga attttatcaa
     1021 aaaacggata aggtttccca ttttattatg tacaagaagc cgccgattaa gaaaccggcg
     1081 gctgtgttca taaaactatt aacgcattaa gaacgaataa ttatcaacat tcttaagagc
     1141 aagaccggtt ccacgtgcta ctgcatgtaa tggatcgtct gctatatgaa attgaatatt
     1201 cattttatcg tacaaacgct tgcttaaacc gcgtaacaaa gcaccgcccc ctgacaagaa
     1261 aataccattt cgcactatat ctgcatacaa ctcaggagga gtttgctcta aagcacttaa
     1321 tatagctgtt tccatttttg aaattgattt atccaaacaa tgtgcgatct cctgataaga
     1381 aacaggtact tccattggca aggctgtcat acgatttgga ccatgaacta tataatcttc
     1441 cggagcttcc tcatcgctaa gttcagttaa agctgaacca acattaatct taatcaactc
     1501 tgcagtacgc tcacctactt taatgttgtg cattctgccc atatattctt gaatatcggc
     1561 agttaaatca tcacctgcga tacgaattga acgattacaa accaaacctc ctaaagagat
     1621 aacagcaatt tctgttgtac caccacctat gtcaactatc atgttgcctt cgggtgcttc
     1681 tacatcaagt ccgataccca tagctgaagc cattggctcg aaaaccatat aaatatcacg
     1741 accaccggca tgttcagaag agtcacgtac agcacgcatt tctacctcag taattccgga
     1801 aggaatacct acaaccattt ttaatgatgg agagaagaaa ctattcttcg aactaatcat
     1861 tttaatcatg ccgcgaatca tcatctctgc agcataaaag tctgcaataa caccatctct
     1921 tagaggacgt actgtgcgga tatgatcatg ctctttccct tgcatctgac gagcttgttt
     1981 accaatagca accaatttgt cagttcgctt gtctagagct actattgatg gttcatctaa
     2041 tacgattttt ccattctgga taataatcgt attcgccgtt ccaagatcca tggcgatttc
     2101 ttgtgtaaat gaaaataatc ccatcttatt ctaaataatt tatttaatac aatttcaaaa
     2161 atatcttaat gtttaaagtg cctaattcct gtcgtcacca ttgctattcc attgttctca
     2221 cagaaatcaa ttgtcaactg atctttaata gatcctcccg gttgaacaac tgccgtgata
     2281 cctgcattgt aagcaatctc tacgcaatca ggaaatggga agaaagcatc agatgccata
     2341 actgcaccat gtaaatccat attgaatgtg ccggcttttt ctatagcttg gcgtaaagaa
     2401 tctactcttg atgtttgacc tataccgcta gcacataatt gtttgttttt agcaagaaca
     2461 atagcattgg attttgaatg ttttacaact ttatttgcaa aaatcatatc ttcaatctca
     2521 gctttgctcg gctgtttggt agttacttgt ttaaaatcct tttctgtttc tacactagta
     2581 tcacgatctt gagatagtaa accatttaaa actgaacgga attgctttgt tggaattttt
     2641 gtctcttttt gaataagaat aatacgattt tttttctgag ataagatttc caaagcatcc
     2701 atatcataat caggtgcaat aattacttca aaaaatattt tgtttatttc ttctgatgta
     2761 gttttatcaa tcggtttatt tgttattagt acaccaccaa aagcagaaac aggatcactg
     2821 gctaaagcat cagtccatgc ctctaaaaga gtattgcgag atgctattcc acaggcattg
     2881 ttatgtttta atattgcaaa tgtagtttca tcaaaatcag caattaaatt aactgctgca
     2941 tcaatgtcaa gcagattatt gtatgatatt tccttacctt gcaattgttc gaacatttca
     3001 tcaaaattgc cgtagaaaaa tcctttttga tgagggtttt caccgtaacg taatgattta
     3061 atatcatctt ttgcgtacct gaaagatgat tgtgcatcac cgtcgaaata attgaaaata
     3121 gctgaatcat acaaagatga tactccaaat gcctctttag caaagaattt gcgttgctct
     3181 aaagtactgt ttgcaccatt ctcgtctaac aaatctatta gcgaagcata ttgattctga
     3241 gatgcaacga ttattacatc agcaaaattc ttagcagctg ctctaataag agatatgccg
     3301 cctatgtcaa ttttttcaat aatatctttt tcagaagctc ctgaagcaac agttgcttca
     3361 aatggataca agtcaacaat tacaaggtca atttcaggaa tttcgtattt agacatttgc
     3421 tctaagtcct ctacattatt acgacgtccc aaaatacctc cgaatacttt cggatgaaga
     3481 gtttttactc tgcctcctaa tatagaggga tagcctgtca agttttcaac agcttgacac
     3541 ggaacgccca atgattcaat gaatgattga gttccacctg ttgaaagtag ttttacccct
     3601 tctgagttta atttcagaat gattttgtct aagttctcct tgtgataaac tgatacaagg
     3661 gcagttttga tttgtttctg ttccattgcg ggtatgtcga tttgggatta aagccatgca
     3721 aagctacaaa atgtaacgac aattatcgcg tacattaata agaaaaaagt aaaaaaacgc
     3781 cgattgttga aagattttac ttgcgtaggt atgttacgaa agaatatgag tatttatgct
     3841 tctcgtccgc cgggtgtttt tcctcttttt cgaagctcca aatttgagta tcaatattag
     3901 ggaaaaacac gtctgattca aagcgaccat ctattatagt taattcaaga cgattggcat
     3961 gcgcaagtgc ttgtgaatag attgaagcac ctccaattac aaaaacatcc tcgtccgatg
     4021 aacaaagagc gaacgcacta tccaaagagt caaccacttc gcatttcgca atttttaaat
     4081 ctttttgacg agaaattaca atatttcgtc tatttggcaa tgcgccattg ggtaaagaat
     4141 cataggtttt tcgtcccata attacagtat gacctgtcgt taattgcttg aaatgcttta
     4201 aatcattagg taagtaacaa agcaactggc ctttataacc tattcctcgc atctcatcca
     4261 ttgcgactat caaaaccaaa tttggcatat tctaattatt taacttaaat atatgttcta
     4321 caacataaaa actccgtact tttgcagtgt gatttttcat agtatttgat tttaaggtta
     4381 acaaataggt actcaggcgt gagtgccttt tgttttttat aggctaaaca gatactgcac
     4441 ctgcaatatg aggatgtgga ttataattca ctagttcgaa atcttcaaaa tgaaaaccaa
     4501 agatatcttt tacatcagga ttcacattca ttgtaggtaa ttcgcgtggt tcacgagata
     4561 attgcaattt cacttgatca atatgattca ggtatatatg cgcatctcca aatgtatgaa
     4621 taaaatcacc cggctccaaa tcacatacct gcgccatcat aagcaataat aaagaatatg
     4681 atgcaatatt aaacggaacc cctaagaaga tgtctgcact gcgttgatac aactgaagac
     4741 ttaacttacc atctgcaaca taaaactgaa aaaatgcatg acacggcggt aagttcatat
     4801 tcggaatatc tgctacgttc caagcactta ctatgatacg tcttgagtcc ggattatttt
     4861 taattgtttc aaccgtttct ttaatttgat ctatatgacc gccattataa tcgggccatg
     4921 accgccattg ataaccataa atgtgaccta agtttccgtc agcgtcagcc cactcattcc
     4981 aaattttaac gccattatct tgcagatact taacattggt atcgccattt aaaaaccaca
     5041 gtaattcgta aataattgat ttaagatgca atttctttgt ggttaacgta gggaatccat
     5101 ctgccatatt aaaccgcatc tgatgcccaa aaacacttat tgtacctgtg ccggtacgat
     5161 cttccttacg cactccttcg ttcattacac gattcaataa atccaaatac tgcttcatag
     5221 attaaatatt atagaggtcg aaacaaagat actaatttta atacattttg taagtagtct
     5281 tcaaaacttt aaactcagta cgtcgattta tttgattagc tatttcttgt tcttctgatt
     5341 ttaaaccttc aataaactcg ttatccaaag gagtatcaac ttttaagaaa cgatattgct
     5401 taacaagtgc cttatcagga acaacaggta cagactcgcc atatcctttt gaagacaacc
     5461 tatccttttc aatgcctttt actgtaagat aatcaacaac tgattttgct ctttgacctg
     5521 acaataacaa gtttgtctca tcagagccta caaagtctgt atgtgcacca atctcaatag
     5581 ttatattcgg attgtctttt agcaattcaa ctaattcgtc aagacctgca gatgattccg
     5641 gagtcaactc tgcagaacca aatttaaaga atatattatt cattttaacc ggcttgctca
     5701 cagaattaag ttgaaaatcc atagtaaagt tttgactctt ttctagatct gccacatcaa
     5761 gaattttctt gctgtttaaa aatcctctgc aagtagctaa aagcacatac ttggtatttt
     5821 tattaataga tgcggtataa gaaccgttct ttttagtctg gatgtttgct atagaaccat
     5881 cattccccac cattctcaca cgagcatcac ccaagttctc tcctttagaa tctgtaatta
     5941 cacccaccac cgaaaaattc aaaacaggtt ttgaaaattt atatatatga tcatagcctt
     6001 tgcggtcatt tctgtttgaa gagaagaatc cccaatcttg ttttccggca aaacttattc
     6061 caaaatcatc aaaatttgaa tttataggct tcaaaagatt aactacgtcc cattcatcgg
     6121 catcagagaa ttttgctttt ggccttttag acgctgcaaa aatatcaagt cctcctaaac
     6181 ccggatgtcc atctgaagag aaatacatta ctccatcagc acggaaataa ggaaacattt
     6241 cttcgccctt tgtattaatt gtaggtccta gattttcggg tatatcccat ccatcaccgg
     6301 tcttagcaac acgccaaata tctcttcccc caaatcctcc atccatatca gaaacaaaat
     6361 ataaatatgc accatcaggc gataaagcag gatgagcagt agttatacta gagtctttta
     6421 agatttgaag tttttgcggt tcagaccatg ctccaccatt acgtttgcac atataaatgg
     6481 cagctcccaa tgtctcacct ttaactactt tgctcagggt ataatacaat tcctggccat
     6541 cagccgagaa agagcaagta ccctcatcaa actcagtatt tatctctcct tctaaaggat
     6601 ttggcgactc ccacttacct tgagcatttg ttttagaaga ataaatatcg ttattgcgtt
     6661 gacctgttat tctgctggtc tttgtttttt ttgcacctct tgcgactcgt gtggaattaa
     6721 aatatagtac atcgccatcg gcagatgcaa aggcaggaca aaattccgaa tacttagaat
     6781 tgaaagtagg atcttttata atttcgtaag cggtgatttt ttcccaatcg gcaattgctt
     6841 tctcacaaga ttctattccg ttttttgcca attcattacc cggtcgcatt tgctcgtact
     6901 gataatacat tttaattgct tccttgtatt taccattacg acgtaatact tcagcgtaat
     6961 aatatgtcag caagctatcc ttgcatccat attttacagc atttttaaat gcagtttcgg
     7021 cacgtttatt gttttcgaca atccgataag cattgcccat tttataagct acatccgcct
     7081 ttagaactcg tttcttagtt ggtacattcg gatatatgct gcgatacatt tcagcagcac
     7141 gataatattc acccaaggcg aactttttat ccgccttggt gattcgggca ttaagactac
     7201 aggaagcgaa taaaacgacc gttagaaggc ctatcaatat tcgcttaatc atcattcagc
     7261 agtttctgga acaataagtt tatatccttt tccgtgaata ttgataattt gaactgtctc
     7321 gtcttctttc aacaatttac gtaatttggt gatataaaca tccatactac gtgcattgaa
     7381 ataattatcg tctatccaaa ttgtttttaa tgtgtagtta cgctctaata tatcattagc
     7441 atttgcgcac aacaacgaaa gtaattcgga ttccttagtt gtcaacttgg ttgttttacc
     7501 attgattgtc aattgttgtt tttgagaatc gaaagtaaag tttcctaatt tgtagacaac
     7561 tttttctttc atcttcttgc tcttgatacg gcgtaaaata gcttcaatac ggaacaacag
     7621 ctcttccata ctgaaaggtt ttgtgatgta gtcatcacca ccgatcttaa atccctcaat
     7681 aatatcttcc ttcaagtttt ttgctgtcaa aaacaaaatg ggtacttctg tattaacggc
     7741 acgaatctct tgtgccaaag taaagccatc ttttttaggc atcattacat caaatacaca
     7801 aacatcgtaa tttgttttca tgaatgcttt ataaccagca tctccgtcag gaagcaaatc
     7861 agtgtcataa cctttggcct gtagatactc acgtaataac attcctaagt tttcatcatc
     7921 ctcacataaa aggattttta ctttttcttc attcataatt ttactttttt aaagtgggta
     7981 ttgttattat aaatttagtc ccttttccta actcgctttc tgctttaatg tgaccattat
     8041 gcttttcaac cattttcttt acatacgcga gccccaatcc aaaaccttta acattgtgca
     8101 cattaccggt tggtactctg tgaaatttgt cgaaaatctt ctttaaatgt tctttcttga
     8161 tgcccattcc gttatcttct accgaaatac acaacaagtc tttctctagt tcattccaag
     8221 tctgtatcat aagatacaaa ggctcatcgg gcttcgaata ctttacagca ttatcaagca
     8281 agttaaaaat gacatttgta aaatgcagct catcaactgt tgcccacgaa tcagcagctt
     8341 ttaaatctgt atttattgtg ccaccagtac tttttacttt aatacgaaaa ctagcaacta
     8401 ctccatcaat aatcttatta acatcagcat ccttaaactt cattattgac ttttcatttt
     8461 ccaataacga aatctgcaaa actttttcaa cttggaaact cagacgcttt gtttcgtctc
     8521 taattatcct cgatgtgctt tttaaaacct ccggtgtttt tattatgcta tcgtcttgca
     8581 acatttgtga tgccaatgat atgctggata taggtgtttt aaattcatgc gtcatattgt
     8641 tcataaaatc cgttttgctc tttgataatt tactctgttt aaacatcagt aaaattgttg
     8701 aaacaaaagt acaaaacaac attaacgtta taatgattga tgttattgcc aaagaagaca
     8761 cactcatcaa aatatattga gagcgctccg ggaaataggc tcttatcaaa ttcatcttgg
     8821 gattatagtc atttggaaat aatatctgcg aataataatt acttgataca catgtagaat
     8881 caaaatcagg gcagatatag acttgacgtc ctaaataatc caacaatata agatgaaatg
     8941 gagtatcaat accattgtta agtaattctt gccgcaataa ttgttcggca tgttccatat
     9001 ctacacgttg ttctataggt ttttcaggtg cttctttcaa ccaacgcata gctgcatcat
     9061 tcaacaatat tttttttcga gcataattat tcttcatacg gctttgcaga ttttctgtta
     9121 attcctgaac tgtatttgtt tgatttacag attttagaga aacagtttgt atgctcggat
     9181 ttgtatctat agatagaaaa gttccattat ctacttttgg tataattgtt ttacgttcac
     9241 tctctttttg taaagcatcg tttaaatatt ctgaggtttc ctcttcttcc aaagttctgc
     9301 tcatttggta caagctacgc ttaactccat cgtcaaaatg ctgttggcgt atttctatca
     9361 aagtactaaa ataattaagc tgcactgcca ataaacctaa aaaggtcagc gccattagta
     9421 ttgcaagaag aatgatagtt ccctttctca tatcttaaac ttaacctgac aaatgtacgt
     9481 tattaacatt tgatttgttt ttttccttac aatcatttaa caattcattt taacatttga
     9541 cgaaatgtta aaattactct ttatttgctg atttataagt tttaagttta ccgggaaata
     9601 agtgataatg acgatattgg caatcaattt cggcattctg tagcatgatt ttatcgttag
     9661 gtttcaatcc tatttgatca aaacctcttt tatttgaaga tataatccat gcgtctgtat
     9721 cggcatattt atgttttaaa gtagttccta tcattccata caaactatct atatcacctc
     9781 cgattctttc accataagga ggatttgtta tgataaaggt tttttcttta ggctggggta
     9841 tattagcaaa atcaattact tgtgtgctga tatatttagt aaaacctgca tttttaatat
     9901 tcgcatctgc cacagatatg gccttagaag atatatcgga agcataaatc ttatgattaa
     9961 acttcttttc tccgctatca tcattatata gcttatcaaa taattcctcg tcaaaatcca
    10021 accatttttc gaatgcgaag ttctttctga aaactcccgg aggaatattt aatgctatca
    10081 tcgcagcttc tattggaatt gttccggagc cacacatagg gtctaggaaa tcgcattgtc
    10141 cattccatcc tgaaagtaaa atcattccgg ctgcaagtac ttcattaatc ggtgcttcgg
    10201 tttgtgctac tctataacca cgtttgtaca atgcatctcc tgaactatct aatgacaagg
    10261 tacatgtgtt gtgagaaata tgaagattaa aaaaaatatc cggattacta acacttatag
    10321 aaggcctttt ccctaccttc tcctgaaaat aatctacaat tgcatctttc gttttatatg
    10381 ttgcgaaacg cgagtgagta aatacttctg aataaacagt tgcctctatc aaaaagcttt
    10441 gcttaacatc aaaaattttt tcccacgcaa tcttttttac gttattataa atctcttctg
    10501 catcctttgc ctgaaaaata gctatcggtt ttagtattct taaagctgtt ctacaatgta
    10561 gattagcttt atacatggtt tcggtagtac cttcaaatag aacagcacgt ctttctatac
    10621 gaacattctt agcccctata ttttcaagtt cttttgccaa cacgctttcc aatccttgga
    10681 atgtcttggc tactaagttt attttatcat tcatgcttgt ttcttctttc agctaccatt
    10741 aataaagcct ggtctttaat tatttcggga atctctattc cacgtaattg caaacttttt
    10801 agccattctg cagtttcatt ctcgcgtaaa gtaagcagaa cgtcttcaaa ttcatcaatt
    10861 tcctcgtctg tatatgaatc agaagttcta cctttgtatg catctaattc ttcatctgca
    10921 aaatatatta tatccttttt tgttgtgata agactttctt tttcgcaaac ttcatgcgct
    10981 ccgcagcatc cttccggtat aactcttttt gatgcttgtt tctgtttttg cattttttga
    11041 gcttcgccat taggattatt agatgctttt acatctttct tgtgctttag tgtaaacttg
    11101 ttaagtatca ctattaaaat tccaaatgct ataaatgcaa taaacagata taaggttcgc
    11161 ataaattatt gttttatatg ttctgcaaaa ttactttgtt gtatcagatc ttatactact
    11221 cgctctcaaa aatatgtccg tccttgttat ccttttgaac aaattcaaac attaaagttt
    11281 tagacaatcc ttcttttaaa gtaaaaggag ctttaaagcc ggttttttta actttatccg
    11341 cagaaaattc ggtagttgca caaaattttt ctactctaac cgaactgata ggtaatttct
    11401 ttcgagttat aaatgccaat aaatcaaagc aatatcccat agatttacct atgaaatatg
    11461 gaatatgaat agatggaatg tgtttgccta aaacagaact aactaatgaa actaaatcgt
    11521 tcatgttata gtcgggcttg tctatataat tatactcttc atatcctttc tgacgatttt
    11581 ctatcaaata ttttacgaat gcaacaacat taccaacgta tgccattgat tttttattca
    11641 ttccgttccc aaccatcaaa aacctacctg aagctatttg tctgagaaga ttataaacat
    11701 ttcctctatt cctttctcca aatattaccg tggggcgcaa tacacttatt acccaatcat
    11761 tgtgtgtttt ataccattct tgtaaaactt gctcggcctg ccatttgctc tttccataat
    11821 ggttaaacgg atcttttatt gagttttcat ttggattctc cttatttaag ccgtatactg
    11881 ctactgagga aaaaaatatt agttctttaa ttccgttatt ttccattgcc tgcaaagtaa
    11941 ccttcattcc ttctacatta gtatcgtagt ataaagaatg cggctttaca tcatcccggt
    12001 gttgggctgc caagagaaca acgcaatttg catcagccaa ttctttattc atttgatcta
    12061 aagatctgac atcagcaatt accgtatatt tctcataaaa tttactctgt aaaagatcta
    12121 agtttttcaa gttgtaagaa cctgcctctt ctagcaaatc aattagtcta gttccaacaa
    12181 aaccactggc tcctataagt gctatttttt tcattcgtat aatttcttta ttttttcttc
    12241 aatttctttt tctacaaaat tctcgattac tctctgcctt gcgtttgatg ccatttcttt
    12301 acaaactgaa tcattcgaca atatataatc taaggctttg gcataagcct cgcaatcttt
    12361 attctgaact tccaatccgg taactccatt aatatttacc caattaacac cagatccatc
    12421 tatagtaaat gttacggcgg gagtattgca atacatagct tctgcaagag caactccaaa
    12481 cgcctcgttt ttcgttattg aagggaatgc gaacacatct gcagcataat aataggccgg
    12541 caacatatca tcaggtattc gtcctatgaa ataaactctg ccgtctttat tcatagattt
    12601 aagactttcc tccaaaacgc cactgccacc tatcaataca acacaatcgg aagaaacaag
    12661 aggcatcgct tccaataaat atcgcaatcc cttataagga acatgacgcc ccacaaaaag
    12721 aactattttt ttattctgat atttagattt aatactttca acttttttct taatttcctc
    12781 attgtattca aacaatgatt tgttaataat attcggaaca acacttactt tttcttttac
    12841 tcgaagcaag ggaagcgatt tttcaatata attcggacta gttgctataa tcttattaga
    12901 tctatctaaa acctttcttt ctatgcccga tacaattcta tacaatttac gctgatctac
    12961 tatgtctgaa tgccaatgaa ttattagctt tgttccttta ggtatagcca aataaacata
    13021 caaagacact aatggatttg ggcaatgcaa atgaatataa tcaggtttaa aactctttat
    13081 taagtttttt aatttaatat aatatgcaaa tgataaaaat tgactcgata tttttattcg
    13141 ggcccctact ctcacaatat caacaccatc aaacttatct gtaatactaa gattatttcc
    13201 actaaagcat attacctttt gttgtacatc gggctctttc tttaatattt caattatgtt
    13261 atggcaaaca tcttcaattc cacctttatg cggaggataa aaattaggta tatgtagtat
    13321 ttttctcatt ctatagtgat gtttttcagt attattgcgg ctgtattctt atacgaatat
    13381 atttcgggta taatgcaatc agggcttttc tcattgctga tgccttcaat ttttttctgc
    13441 aaatcgtttg aatttccatt ttcaaaaaat attacaggca attttgaata tacttcttta
    13501 aatacaggta tatctgatat aattactttt gtatgtaaaa ataatgcttc taatggaggt
    13561 attccaaaac cctcatacct tgatggttgt atcaaagcct cagcatcact tataagagaa
    13621 tacagttctt catcactttt ttgaccaaca aatacaactt tattctcatt taaagaactt
    13681 tcgttaagta attctttatc agaagtcctc atatcatcgt atttgccaac aacaataagt
    13741 tttacatcag acattgcttt tacagccgac aacagtatat cgagtccttt ataacgcttt
    13801 atattaccaa cgtacagcaa ataattttta tataaatcag aactcctttt tttattcttc
    13861 cattcaaata aatcatgcct aatgccatta taggcaacca caatttcttt gtttgtatta
    13921 aaatgctttt tgatccttgt ttttgagaac tctgatacag taaagatttt ttttgatgcg
    13981 tatatagccc gacaatagaa caattttctc accaataaac tcaatccgcc tactaatccc
    14041 tttacatcta aaaaaaccac atcgtgaatg gtcgagtaga caggaacctt tattccaaaa
    14101 gggataccaa atgttggact ataaaagaca tcacaattgt ttacctcttt tacaggaaac
    14161 gaaaacattg ccccaaatga aaatacagaa cttgaacaag atacaacatt acaattttca
    14221 ccaaatttca atttactgag tttcttctca tcacctatca atacaaaaga gtgggctttg
    14281 tttgtttcaa gccaataata tagaatattt tcaagatata ctccaattcc gctcattcca
    14341 agcattctac aatctacagc tattttcata aatctttcaa taatagtttc cactcttcca
    14401 taaccggagc taaatcaaaa cgcatactat agcgaacggc attttcagag aatgatttaa
    14461 gtaaatttgg agaagacaca agaaaatcta ttttttctgc caaagctttt atatccattg
    14521 gaggtactaa gaatccattt tccccatctt tcactatgtc tcttggaccg gtttcacaat
    14581 caaaactcac aataggcaaa cccatagaca ttgcttcaat caatactagt ggcaatcctt
    14641 caaatcttga tgataaaaca tataatgatg cgtttaaata ctccgacacc atatctgtta
    14701 caggttcccg catagttacg ctatcgtgca agtttagtga agtaatcatt gatttaagtt
    14761 cgctttcttt agaaccgctg ccaataattt ccaacttcca acctaagtta acacaattgc
    14821 aaagtttcca tgtttgtaac aacaaatcga aacctttttg atcttctaaa cgacccactg
    14881 ctaaaacttt tttagatgac aatgtcgttg tttgtttttt ctctatagta atgggattta
    14941 caatacaagt aacgcgttgc gctttatatt cattttgata aacttcacga tctgtttcgg
    15001 ttaaaacaac tacatgatca gcaaattttg aaactaattt tcgggcaagc ggggatgtta
    15061 ttggattcca atttacatga gcgttaaaat gctcccatga aattagtttt atatctaatg
    15121 caaatttcgc aggcaggctc cataaagaca ttgcgctaca tacatcaatt atacaattcg
    15181 gtttttcacg ttttagcaat tgacgcaaac gccatactgt tggcaagtat gttttatata
    15241 tgtttatgga ttctttgtta ttgagaggaa taacatttac agtttcgcga agtttaaaaa
    15301 aaggtacgca cttttgtaaa catattattg acacctcatt accttctcta gataattcat
    15361 tggcgataac tgtagtaacc cgttctgttc ctcctgcaga ttccaaagat tttatcaaaa
    15421 aaaatatttt catgatgaca atattctgtt atagttcact tctttaaacc catttggggt
    15481 acatgtaaca atgctttacg caatgcaaag gtaaaaaaat ctattttata tactcttttt
    15541 accatttgat atatttttat attaaatgca aacttttgcg gcaaaagctt tatccgactc
    15601 atcaaagacg gcttgtttac aatagtagat cctatcacat ttccgctatg ttgtctatac
    15661 aaaagtgttg cattttttat tggtacaata attccatttt tagcaacaca caaagctatc
    15721 caccaatcat gcataattat attctcaggc attggtaata tatattgctt tgttaattca
    15781 ttaaaagcca ttgtacatcc cattaaacga tttgatactg ttaaaacatt ttctttgatg
    15841 gcgtcttctg gattttgtcc ggattgagca aacgacgact caactattac attcaagcat
    15901 tcgtctacta ttttaatatc agtataaacc agcaccggaa cagatggatt agctgattcg
    15961 gcagcattta ttgaattcaa cgacaattcg actttacctt ttatccaaac atcatcttga
    16021 tctgataaaa aaatatagct tcctgttgca tgttcaattg cattggcaaa attagtagaa
    16081 actccatttt tacccggatt ttcaaaaatt ttgacgcgtt tatcattcaa agcctttatt
    16141 atatcaatag ttttatctgt agaatgatcg tcagagatta ttatttcatc atcctcagaa
    16201 agttctgaca aaattgatat caattgttct ttgatgtact tttctccatt atatgttgct
    16261 aaacaaacag aaatcattct ttgatagatt tattgtactc ttcaatattg tttttcatat
    16321 attctttctg aaaaaagtaa ctcgctttac gcatattttc ccgtctaatt tcattttcac
    16381 ttttatagaa ccttgaaaat atcgtggtag aattaagtaa ttttgcttca ctataatagt
    16441 tagatagcca aataccgcgt gcataattaa ttgaatataa gtaaataaga cataccgcca
    16501 atttactaat ccagatttgt ttgctttttg attgagataa aataggaatt agataaacga
    16561 tcaccaccgg taaaaaataa gacctcaagc gatttaaaaa gaaccaatct ctataagaaa
    16621 tagatacaac aataaaaaac aatgctacta ttttggcata ttttgagttc aaatctttat
    16681 tttccaccga aaatattaat agcaataata ctatggcata cacaaaaaat attatttgaa
    16741 atgttttagt gtacataagg tgatgcttaa cagattcgat gacatttgtt gaagccggag
    16801 tttttgaaat aatccaaatt attatctgat ctattggcaa aaataaaaag acaagcaata
    16861 aacccgaagc cactagaaaa atggttttgc ttacttttac tcgctgaaaa aaaagcaaca
    16921 aaatcataag catatataca aatacagccg atttgtgaat aaaaaaagat aacacagcaa
    16981 gtaatacaca aacaaaataa cttcgtttat aatatgcact cagcgaaaac aagaaaagag
    17041 ctacagtaag attttgtcta aattcaaaga ataacaaatt ggcatcgaat aaaacaataa
    17101 ggaataatgc aaatgtttta tacttgttta atgacgtaaa taaattataa attgcagcaa
    17161 aatatattgt agtgattata actgagcaaa accaataact acaccctata cttttaagaa
    17221 aagaaaagaa aaaaataaac agacgctctg tatcaagcga acttttggga ggattaataa
    17281 ggtcttttat tggagatgat aaagaatcgt ataagtttac ataaatggca atatccggtc
    17341 cgtaataata cttaattgcc acaataaagt aacttatcgc aaatgctata aaatacactt
    17401 gcttgttcat tctctcattg ttcgggaata taaactcgat caaagacaat gggaacagaa
    17461 tcaatatggg caaaagcatt aagttcattt ccaggagcat aaaattttat ttctactcca
    17521 aattgacata cccacagcta ccagaaattc tgtgagcaat aaagcaacag cggctcctac
    17581 tgaatcgaat ttcggtacta atataaaaac cagaactaaa gcaaatgcgg ctgccgcaat
    17641 atatattctt ttaaacgtaa tcaaattttc gctatttcca actgccacga ttttcaactg
    17701 tccattcacg cctccaatgc caataaataa tggcacaaaa gccataattt taaagagaag
    17761 ttctgcatca gcataatcgg aacccagcaa ttcgacaatg gtatcagaaa agaaaaacat
    17821 accacctgct atacacaaca taccgactat aaccagccaa agtaacttgt cgcttagatg
    17881 tatcgctccc aacttatctt gtttgccaag tctgctgact tttggataaa aagactgagt
    17941 aaccggcatc agtacgagta aactcattgc cctcatcaat ttatctactg cagcatattt
    18001 ccccacttca tccggacttg caaaataacc aaggattact acaaaacttg ctgtatatat
    18061 gcttgttgcg gcattcgata aaaaaatagg gtaactttcc tttatttctt gcttaacaga
    18121 aactatctta caacgaatta gcgacaccca ttttttatgc catataatta ttgcatttat
    18181 tattgatgaa actatatata cagaacttaa aactattgct gccaacaaat aatcgttggg
    18241 atattttact ataaaaaagg taatcggaaa tattgataat ttacaaattg tatttacaat
    18301 tgatactaaa cgtatatttc ccaaaccctg aaacaaccat acaaaagaga aggcattgcc
    18361 aacaaccatt aaaaacacaa caaacatagt tgtaacatat accttaaact gggggataaa
    18421 agaaaacaaa accaatatca aaaagctgat tgccaataat aatatcttag cgtaaattgt
    18481 tgctgaaaat atttcgttta gctttgattt attatcctta tacaatgcta ctcgctttgt
    18541 tgcggttaaa ttaaatccaa aatctacaat aagcattaga taggtgcaaa atgacatcga
    18601 aaatcctata taaccgaatt tttctgcacc taacgttacc attaaataag gatatacgat
    18661 tagtggagct acataattaa gaccctgcaa agcgatcaaa taaaatacat cttttacttc
    18721 tcgcttatta ttgtttatta gttctttagt tctcaaaaat ggggcgtttt aatatgatct
    18781 cgcaaatatt actctttgtt ttgatggctt gtccgttacc atacacttgc cggggatttg
    18841 atctccttct aaaggtatgc aacgtattgt cgcctttgtt tcttctttta ttttttcttc
    18901 agtctctgtc gttccatccc aatggcataa caagaatcca cccttttcta tttcttcctt
    18961 aaattcatca taagaattaa cttcacgggt aacagaagaa cgataatcta aagctttctt
    19021 gtatatatta gactgaatat cagacattaa gtctcttaca tattgctcta ttccgtctaa
    19081 actacgtgtt tctttttctt ttgtatcacg acggaacacc tctactgtat tgttttccaa
    19141 atcgcgtgct ccaattgcta aacgcacagg aactcctttt aactcatatt cggcaaattt
    19201 aaagcccgga cgtttattat cagcattatc atatttaaca gatactccta ataattttaa
    19261 attagcaata attgtatcaa cctttgcatc aatatctttc aattgctcat cactcttgta
    19321 aataggaaca ataaccactt gataaggagc aagatttgga ggtaatatta aaccatcatc
    19381 atccgaatgc gacataataa gagcgcccat caaacgggtt gatacacccc atgatgttgc
    19441 ccaaactaat tcttgtttat tgtctttatt cataaatgta acatcaaaag ccttagcaaa
    19501 gttttgacca aggaagtgag atgttccggc ttgaagagcc ttaccatctt gcattaatgc
    19561 ttcaatacaa tatgtttcaa cagctccggc aaaacgttcg tttgcagatt taacaccttt
    19621 caaaaccggt atagccataa agttttctgc aaaatcggca tacacattca acattttaac
    19681 agcttcttct tctgcctcct cttttgttgc atgggctgta tgaccttctt gccacaaaaa
    19741 ttcggcagta cgtaagaata atcttgtacg catctcccaa cggactacat tagcccattg
    19801 attgcacaaa ataggaagat ctctatatga atgaatccag tttttatatg tgctccaaat
    19861 tattgtttct gaagttggac gaacaattaa ttcttcttct aattttgctg caggatcgac
    19921 aacaactcca cttccgtccg gatttttttt aagacgatag tgagttacaa cagcacactc
    19981 tttagcaaag ccttctacgt gatctgcttc tttgcttaaa aatgattttg gaataaataa
    20041 agggaaatat gcgttaacat gacccgtgtc
//
LOCUS       DAOPID010000006        18001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_3471_length_18001_cov_4.149281, whole genome shotgun sequence.
ACCESSION   DAOPID010000006 DAOPID010000000
VERSION     DAOPID010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 18001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18001
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3471_length_18001_cov_4.149281"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PK641_00620"
     CDS             <1..391
                     /locus_tag="PK641_00620"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HPG54892.1"
                     /translation="QIVNQAQSTNESGWKAFEDNKNRYALINNILDEVVTPYREYFYT
                     YHRLGLDEMSLSADKSRTRIVEGLPILKTVYKSRPSVIIINEFVETKMDEIINIYSKA
                     SSQERKDAYEILSYIVPTMQNSLSALK"
     gene            399..2051
                     /gene="recN"
                     /locus_tag="PK641_00625"
     CDS             399..2051
                     /gene="recN"
                     /locus_tag="PK641_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HPG54893.1"
                     /translation="MLRSLHIENYVLIKSLDLDFKSGFSVITGETGAGKSILLGALGL
                     VLGNRADLKTISDGQEKCVIEAVFDISNYNIKKLFEDNDLDYYDECIFRRELLRSGKS
                     RLFLNDTPVSISILKAIGDKLIDIHSQHSNLLLKDDSFQLSVLDCIADNKDIRNKYRE
                     IYDKHITVSKLLKNTIELAASDKKDLDYIEFQCKQLCDAKLRADELPELESELLYLEH
                     ATELKTALAQTEDALLNETGIISLLRIAENQTQTASNFQAELQALHDRISTCTIELKD
                     IADDISKRCESIEMNPERLEQVRERLDLIYTLLQKHKVADVSELLDLQKVLENKISKL
                     NCSDENIDKLKKEENLLNIELQKLATALSESRKGTIKQIESHLTASLSNLGIPNANFV
                     IEINTTDTPSRSGIDKVSFLFSANKLQKPQAVAQIASGGEISRLMLCIKAMIAKSENL
                     PTLIFDEIDTGISGEVAAKMGNILKEMGLHSQIICITHLPQIAAKGSSHYMVYKKDEE
                     GTETHVKLLNQNERVEEISKMLSGTAISSAAIENAQDLLNNN"
     gene            complement(2027..3058)
                     /locus_tag="PK641_00630"
     CDS             complement(2027..3058)
                     /locus_tag="PK641_00630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPG54894.1"
                     /translation="MKKVAVIILNWNGKSFLKKYLPKLIEYTPYDDADLYVADNASTD
                     DSVDFLKQNFPTVKLIEFKKNYGFAEGYNLAIAKVDTEYVVLLNSDVQVTPDWLQAPI
                     DYMDKNPRVAACQPKILQINNRNFFEFAGAGGGYIDILGYPFCRGRILSDIEYDDGQY
                     NDVRQVFWASGACLIMRRDDFQDYGGLEASFFAHQEEIDLCWRLNARGRLVMVVPQSV
                     VYHVGGGTLSAENPHKTYLNFRNNLCMLYKNVPMLSLWWVWIIRFFLDYLAAIIMLLQ
                     GKKKDAAAAIKGRLAAYSMCCSLKEKRKENMHKYITKRIPVIFKGSILFSYYVLGKNK
                     FSQLLFSKS"
     gene            complement(3030..3908)
                     /locus_tag="PK641_00635"
     CDS             complement(3030..3908)
                     /locus_tag="PK641_00635"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPG54895.1"
                     /translation="MKTLFSLIARLPWCVLFALSDFFYVIVYYIARYRRKVVRKNLVN
                     SFPEKDLKSIIKLEKKFYHHFCDLFVETIKLFHISVPEIQKHFKFTNIDEIKASLAAG
                     DRLFMFSAHHASWEYLISLSIYIDFPSIHFYKQLTNKTFDAMMLEMREQFGSISIETN
                     NGMRELAKRDKEGGAYITCFIVDQAPLPQSRQYWTKFLNQDTPFIFTAERMARKFSYK
                     ACYSRVVKVKRGYYEVTILPMCDNVANTAQNEITQMYAEMLEADIRNQPECYLWSHNR
                     WKYSRKDYEKSSSNNS"
     gene            complement(3905..4195)
                     /locus_tag="PK641_00640"
     CDS             complement(3905..4195)
                     /locus_tag="PK641_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG54896.1"
                     /translation="MSSEKNLSAYDDDDAIAFILKNISSDYQSFFEDDDIQYFLDLMY
                     EMDEKFIVDEDELISKIIKESKKDGMDKFTAENVTALLDAENKYSKSIGLFE"
     gene            4285..5106
                     /gene="panB"
                     /locus_tag="PK641_00645"
     CDS             4285..5106
                     /gene="panB"
                     /locus_tag="PK641_00645"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HPG54897.1"
                     /translation="MSIHQIEDQRKVTTHRLLEMKMRGEKISMLTAYDYSMATIIDEA
                     GMDVILVGDSASNVICGNVTTLPITLNEMIFLARGVARAAKRALVVVDMPFGTCSGNP
                     RMSLEAAIRIMKETGADSLKIEGGKEVVEDIKKIIDAGIPVMGHLGLMPQSINKYGTY
                     TVRAKEKAEADKLLEDAKLLEEAGCYGIVLEKIPAKLAQKVAQSLSIPVIGIGAGGYV
                     DGQVLVMHDMLGISQGFSPRFLRRYADINGIATAAVQQYIKDVKSKDFPNESEQY"
     gene            complement(5178..7832)
                     /locus_tag="PK641_00650"
     CDS             complement(5178..7832)
                     /locus_tag="PK641_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1939 domain-containing protein"
                     /protein_id="HPG54898.1"
                     /translation="MEKMYFRAKKICFLFLLALFCSAPLSADVEKKVVLQAFWWDYWH
                     NDYPNSYANYLTELAPRLKELDINAVWVPPFFKNPHSGSVGYSPFDHYDLGDKYQKGS
                     NTTRFGTKDEVLRMIAVMHANGIEVIEDAVLNHVDGAGSEKDGNGGEDPGARDNKWKN
                     FRYVSYDTPATDGSESDYWSRSGRWAKNWPNFHPNQAHDCNTGDWCSDWWGPDICYYE
                     GAYGQSSNAVGYNPTQSANYMRNQARDWLIWFTKQTAVDGYRWDAVKHFPEWAQQDFV
                     YNVKYSLPSWCAGGEAMFNVGEFIDGKSRIDQYCNDVKYANGGSEFLMGAYDVPLRGT
                     IKNIVSGGGYANLSDVPGSQLTMRYENYATQKVHRSLNYVNSHDTFRPEFDEDGKYIG
                     WDGNNETGGHIDPFDKRLGMAYAIAMALDGNISVYMEDLFNLSNGKRWTHDPKNEEEL
                     PVRDAVKNLIWCHQNLDFKYGDYKVRWQDGDLLIVERAGKAIIAITDNGASDREEWID
                     TDFRNVELKDYSGGVSGNRTAYSDGRFCAKVTNTGTGLGYSVWAPANKETNYTPYRSP
                     ITVQEWEMANDLGDSHCESLGQGGALPENSTAQRLVGKIYAENDKELSIVMHPADEGN
                     DITLAIYDLDGNVLSKANGTQDVTLTYTPTQVGWIAIKIWNSSTNNIAQKAWVKATYE
                     APAVVTETMSDVADTRASIWTGNKGTTDWTDCANWEQGMVPTTSSIVVIPDNGMVRPI
                     INTNVTISELIIEKGKGSAESPDIMVNATLNVTGLSKSIEGNSFICGEGAVYMNDKEG
                     NFDLCATEVSEINETSFSIFPNPATSQITITSDYLTQTELIIYDMSGRIVSKNIFDET
                     IANIDISTLSDGVYYVRLNGKIEKFIKR"
     gene            7978..8502
                     /locus_tag="PK641_00655"
     CDS             7978..8502
                     /locus_tag="PK641_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HPG54899.1"
                     /translation="MKQYLLLIFAFTWALLPLSAQIDQGAMLDAINELRAEGCTCGTS
                     ELKPVTSVSWNKDLAEIAKSYSDHLKDNNKSSKNNFLFLSHVGIDGSTLESRLAEANF
                     NTKFACENIAFIEGNEDTAIDYWLNNPESCQNIMSKQSTALGAARSGDFWVLILAQPI
                     IKSKKDKTQEDITE"
     gene            8529..9779
                     /locus_tag="PK641_00660"
     CDS             8529..9779
                     /locus_tag="PK641_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPG54900.1"
                     /translation="MQTIDNYKFAGKKAIVRVDFNVPLDENGNITDDTRIRGALPTLK
                     KVLNDGGSLIIMSHMGKPKGKVAAKYSLKQILDSVSKTLGTAVKFAPDCAKASQAAAE
                     LKAGEVLLLENLRFYAEEEGKPTDIEKEDPRYEEAKKAMKASQKEFAKTLASYADCYI
                     NDAFGTAHRKHASTAVIADFFGTDDKMLGYLMEKEVNAVDNILSNIKRPFTAIMGGSK
                     VSTKIGIIENLMDKVDNLILCGGMTYTFAKALGGEVGKSICENDKLDLALDIMKKAKE
                     KGVNLVLGTDCVAADSFSNDAKTKIVPSNAIPEEWEGLDAGPETRKAFADAIEDAKTI
                     LWNGPAGVFEFDNFTGGSRAIADAIAKATAKGAFSLIGGGDSVACINKFGMADQVSYI
                     STGGGALLEAIEGKVLPGIAAIKG"
     gene            9922..10197
                     /locus_tag="PK641_00665"
     CDS             9922..10197
                     /locus_tag="PK641_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG54901.1"
                     /translation="MIFNCVSVFLAISIGKLSDKIGKDLKGTGFGLYHAILGITLLPA
                     SLIAGLLYDNVNSDAPFYFGSLMAMLSVILMIIFVISKPKKQIINKD"
     gene            10211..10894
                     /locus_tag="PK641_00670"
     CDS             10211..10894
                     /locus_tag="PK641_00670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HPG54902.1"
                     /translation="MDASNIIRFLNELRANNNKNWFDANRKWYEDVKSDFFEFSKKLI
                     IEVSKFDSRINNLDIKDCTYRINRDIRFSNDKSPYKTHFGTFICPKGKKSGFGGYYFH
                     IEPQGGDYLGNHLMAVGAYCPEKDELESIRTEIYDNPEKFIHALKQAKGFTFDESAKL
                     KKNPKSYPADFKYIEYLKYKNYCIEKVLSEKDLQDPNLLEILLDNYKSAHAFNEIINK
                     SIEYIKENA"
     gene            complement(10903..11325)
                     /locus_tag="PK641_00675"
     CDS             complement(10903..11325)
                     /locus_tag="PK641_00675"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HPG54903.1"
                     /translation="MELRVKNIKGKPYIFDACRMKWVAATPEEKVRQGFCAYLIDSLN
                     YPAVCINNECIVEINGLKQRCDTLVYKQMTPIMLVEYKAPSVIIDKSVFEQVMRYNSQ
                     FHVPYIVVTNQSQTYCCKMDYDNMSYDFIKEIPMWSDL"
     gene            11376..12383
                     /gene="holA"
                     /locus_tag="PK641_00680"
     CDS             11376..12383
                     /gene="holA"
                     /locus_tag="PK641_00680"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HPG54904.1"
                     /translation="MAKQEVTYQDILQQLQLRQFSPIYVLMGEEPYYLDKLTDYFESN
                     VLSEDERLMNQVVYYGKDSQVETIINEAMQFPLMSPYRLVILKEAQTLDNKNKLDLLE
                     HYLKQPAPSTILIITYKYGTIDRRKKWIQQAEKFGVVLDSKKLRDYQVTTFIESLARS
                     VKLSIDEEAVAMLIEFIGTDLTQLESVINKFNILLTTNRRITTEIIEKTIGISKDYTA
                     FELINALITKDVVRSNRIIMHTTSAIQQIISVLFNYFSGLMIYHYLQNKSKDNVVAQL
                     RINPYFVKDYELGARNFSKMKTFNTIGYLREYDAKSKGVNNISANDLDLLKELIYKIL
                     H"
     gene            12458..13831
                     /locus_tag="PK641_00685"
     CDS             12458..13831
                     /locus_tag="PK641_00685"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPG54905.1"
                     /translation="MDINSYSSYFFVGIAGTGMSAIAQYLNGIGKQVSGSDRLFSTTE
                     KMLSQTQFEAQGISCYFQDGSGISNQTDVVIVSTAIEESNIEYQKALKLNIPIVKRSE
                     LLAAISDSIKTIAVGGTSGKSTTTAMIFHILEKCGKSPSLITGAGLSDLQDKGLPGNA
                     WNGKGEWLVIESDESDGSIVNYHPEIALLLNIDRDHKEFEELIDLFSIFKSHTKSTFI
                     VNQDFELTRKLSQDIKNDFGTQNNAGITGSNFEQKGFSINFQIDGKNCVVPLIGRHNM
                     ENALAAIAAAKAVGISLNDAINALADFKGIYRRAQLVGSRKDTNQYVIDDFAHNPAEV
                     ASAIRACQTIAPKVIAYFQPHGFGPLSFMHKELGEMVAKTLRNEDIFLIGDVYYAGGT
                     VDKNISPVIVSDAINKTGKNAKFVGNKQQALKAILEELNNNTVILTMGARDPKLDVFA
                     KEIFEAL"
     gene            complement(13898..14851)
                     /locus_tag="PK641_00690"
     CDS             complement(13898..14851)
                     /locus_tag="PK641_00690"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007764101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HPG54906.1"
                     /translation="MNIAEKLNNSDHPLFTFELLPPLKGRGIDAIYDTIDNLLEFNPS
                     YINITSHAADIVYKNMPDGSISKMVVRKRPGSVATAAAIKYKYNIEVVPHIICSGLNR
                     EEIENLLIDLNFLDINNIFVLRGDPQKGSRIFIPKEDGHDHTTSLMQQVNMMNSAKYL
                     DDSLKNPKATQFSMGVACYPEKHIEAPNLATDIQYLKEKVNLGAGYAVTQMFFDNQKY
                     KDFVKSCRASGINIPIVPGIKPIAALNDISLLPQIFNIDLPEDLVKEIQKCKTKEQVR
                     ELGVEWSVAQAKDLLAFGVPSLHFYSLGESDNLKKICKAVF"
     gene            complement(14903..>18001)
                     /gene="metH"
                     /locus_tag="PK641_00695"
     CDS             complement(14903..>18001)
                     /gene="metH"
                     /locus_tag="PK641_00695"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HPG54907.1"
                     /translation="IHRMYIEAGADIISTNTFTANEGSDIRNVNLRYASLAIDEARKC
                     DKRNIYVAGSIGPTNKTLTMSDDITFDEMSASYEIQIQALKDAGVDAFLFETCFDTLN
                     LKAALCAAHRVDKEMPLMISATIDKNGRLLSGQTLEAFVVAVTPFTPLSIGLNCSFGA
                     NDMFPYLKNLSELTDAYISAYPNAGLPNELGQYEQTAEIMIEQIRPYFESNILNIVGG
                     CCGTTPEHIRLIADMAHNNVPRIPQNQARLTRLSGLEPLTIEHFTNIGERTNVAGSKK
                     FARLIAENKYEEALLVARKQVDGGASIIDVCMDDSMLDAKTCMAKFLHLLGADPYIAK
                     VPIMIDSSKWEVLEEGLKHVQGKSIINSISLKEGEEVFLSHATHIKNSGAAVVVMAFD
                     EEGQASTYERRISVCKRAYDLLVQKAGFKPEDIIFDPNVLSIATGLPEHDSYAVDFIR
                     TVEYIKKNLPYAKVSGGISNLSFSFRGNNKVREAMHSVFLYHAINAGMDMAIVNPEML
                     EVYDNIPKELLDKVEAVVMNTHPNATDELITYASSFQNDNTKKEEMTDAWRILSVEER
                     LEYALINGTTEYVESDINEALNTYPPLEVIESILMKGITKVGTYFGEGKMFLPQVVKS
                     AQVMKKAVSYIMPHIEKDDAKTIRKKIVIATVRGDVHDIGKNIVSVVLSCNGYDIIDL
                     GVMVPKELIIKSAIENNASMVCLSGLITPSLHEMEEVAKEMQINKLNIPLIIGGATTS
                     EEHTAYYVDPLYDGAVVHSKDASTNVSIASKLLNDDNYKASIKAKYSELRDKIKNKQT
                     KSVSKAVNIDWTTEKVYVPTFVGKNVLTDYPIDEIIPYINWTAFCAAWKVKGEHAESL
                     IEEAKCFLNENIIKIKANAVVAISPVKKENESIEINGYKFNFVRKDEVCLTDFLSPNL
                     DYVGLFAATINVTDLLETLKKSGDDYKAMMLQLLADRLVEAFSELLHTKMKDEWWGFK
                     NGIRPAIGYPSLPDHSMKREFFELLDVTNSIGVTLTETNAMQPVSSVCGFYFACDKAK
                     YF"
BASE COUNT         6037 a         3317 c         2948 g         5699 t
ORIGIN      
        1 acaaattgta aatcaggcac agagtactaa cgaatcagga tggaaggctt ttgaagataa
       61 caagaatcgt tacgctctaa ttaacaatat attagacgaa gtagttaccc catatagaga
      121 gtatttttac acctatcatc gccttggttt agatgaaatg agtttatctg ccgacaaaag
      181 tagaacccgt attgttgaag gcctgcctat acttaaaaca gtttacaagt caagaccatc
      241 tgttattata atcaacgaat ttgttgagac caagatggat gaaataatca acatctactc
      301 aaaagcttca agccaggaac gtaaagacgc ctatgaaatt ctgagctaca tagttcctac
      361 tatgcaaaat agcttaagcg ccttaaaata attttttcat gcttcgtagt ttgcacatag
      421 aaaattatgt tttgatcaaa tccctagatt tggactttaa aagtggattt tctgttatta
      481 ccggtgagac gggtgcagga aaatcaattc tattaggtgc actaggttta gttttaggaa
      541 acagagccga tttaaaaacc atttccgatg gtcaagaaaa atgtgtaatt gaagccgttt
      601 ttgatatatc aaactacaat attaaaaagc tctttgagga taatgatttg gattattatg
      661 acgaatgtat attcagaaga gagttattac gcagcggcaa atcacgttta tttcttaacg
      721 acactcctgt aagcatatct attttaaaag ccataggcga taaactaata gatatccact
      781 cgcaacatag taacctgtta cttaaagatg attcgtttca attaagtgtt ttagattgca
      841 ttgctgacaa taaagatatt agaaataaat atcgcgaaat atatgacaaa catattactg
      901 tatcgaagtt attaaaaaac actatagaat tagccgcatc agataaaaag gacttagatt
      961 atatcgagtt ccaatgcaaa caattatgtg atgcaaaatt acgtgcagac gagttgcctg
     1021 aattagagag tgagctgctg tatttagaac atgcaaccga attaaaaaca gcattggcac
     1081 aaacagaaga tgcattactc aacgaaacag gcataatatc attattgcgt attgctgaaa
     1141 atcaaacaca aacggcatct aatttccaag cagaattaca agccttacac gacagaataa
     1201 gtacttgtac aattgaactg aaagacatag ctgatgacat atcaaaacgc tgtgaatcaa
     1261 ttgagatgaa tcctgaacgt ttggagcaag tacgcgaacg tttggattta atctataccc
     1321 tacttcaaaa gcacaaagtt gcagatgttt cagaattgct agaccttcaa aaagtattgg
     1381 aaaataaaat ttccaagctc aattgttctg atgagaatat agataaactc aaaaaagaag
     1441 agaatctatt aaatattgaa ttacaaaaac ttgcaacagc actatcagaa agcagaaaag
     1501 gcactattaa acagattgaa tcacatttaa cagcaagttt atcgaactta ggtattccga
     1561 atgcgaattt tgtaattgaa ataaatacaa ctgacactcc aagccgcagc ggaattgata
     1621 aagtttcatt cttgttttcc gcgaacaaat tgcaaaaacc acaggctgtt gctcaaatag
     1681 catcaggtgg tgagatatca agactgatgc tttgtatcaa agctatgata gctaaatctg
     1741 aaaatctacc tactttaata tttgacgaga ttgacaccgg catatcaggc gaagttgccg
     1801 caaaaatggg aaacatttta aaagaaatgg gattacattc tcaaataata tgcattacac
     1861 accttcctca aatagctgca aaaggtagta gtcattatat ggtatataag aaagacgaag
     1921 agggcactga gacacatgtc aagctactta atcaaaacga aagagttgaa gaaatttcaa
     1981 aaatgttaag tggtacagct attagcagtg ctgctataga aaacgctcaa gacttactga
     2041 ataacaattg agagaattta tttttcccta aaacataata agaaaataga atactgcctt
     2101 taaatataac cggaattcgt ttagttatat atttatgcat attctcttta cgtttttctt
     2161 tcaaagagca acacattgaa tacgccgcta atctgccttt tattgcggct gcagcatcct
     2221 tcttctttcc ttgcaaaagc attattatgg cagctaagta atctaaaaag aatctaatga
     2281 tccaaaccca ccacaatgat aacataggta catttttgta caacatacac aagttatttc
     2341 tgaaatttag atatgtttta tgtggatttt ctgcgctaag agtacctcct ccaacatgat
     2401 atacaacact ttgaggcacc accataacta gtcttcctcg tgcatttaat cgccaacaca
     2461 aatcaatctc ttcttggtga gcaaaaaacg aagcttccaa gcctccatag tcctgaaaat
     2521 catcacgtcg cataattaag caagcaccac ttgcccaaaa cacttgtcgc acgtcattat
     2581 actgaccatc atcatattct atatcagaca aaatacgacc acgacagaag ggatatccta
     2641 ggatgtctat atatcccccg ccagcacctg caaactcaaa aaaattacga ttgtttattt
     2701 gtaatatttt aggttggcat gccgcaacac gtggattctt atccatataa tcaataggcg
     2761 cttgcaacca atcgggagta acttgtacat cagaatttag caatacaaca tattcagtat
     2821 caaccttagc tattgcaaga ttataacctt ctgcaaagcc atagtttttt ttaaattcta
     2881 taagttttac agtaggaaag ttttgtttta aaaaatcaac agaatcatca gtggatgcat
     2941 tatcagcaac ataaagatct gcatcgtcat agggagtgta ttcaatcagt tttggtaaat
     3001 atttttttaa aaacgattta ccattccaat taagaattat tactgctact tttttcataa
     3061 tcttttcttg aatatttcca ccgattatgc gaccacaaat agcattcggg ctggttacga
     3121 atatcagctt ccaacatctc ggcatacatc tgagtaatct cattttgagc cgtgtttgct
     3181 acattgtcgc acattggaag tatagttact tcataatacc ctcttttaac ttttactacc
     3241 cgactgtaac aggccttata actaaatttt ctagccatac gttctgcagt aaagataaat
     3301 ggagtatcct gattcaaaaa cttcgtccaa tattgacgtg attggggaag aggagcttga
     3361 tcaactataa agcaagttat ataggcacca ccttctttgt ctctctttgc caattcgcgc
     3421 ataccattat tagtttctat tgaaattgag ccgaattgtt ctctcatttc caacatcatt
     3481 gcatcaaagg tcttattagt caattgtttg tagaaatgga tagatggaaa gtcaatataa
     3541 attgataatg atataagata ttcccaagaa gcatgatgag cagagaacat gaataatcta
     3601 tcacctgcag ctaaagatgc ttttatttcg tcaatatttg taaacttgaa atgtttttgt
     3661 atttcaggaa cacttatatg aaacaatttt attgtctcaa caaacaaatc acaaaaatga
     3721 tggtaaaatt tcttttcaag ttttataatt gacttgagat ctttctcagg aaatgaattg
     3781 actaaattct tacgaacaac cttacgtctg tatcttgcta tataataaac aattacataa
     3841 aaaaaatcag aaagcgcgaa aagtacacac caaggtaatc tagcaattaa agagaataat
     3901 gttttcattc aaacaaacct attgatttag aatacttgtt ttctgcatct aataatgctg
     3961 ttacattttc tgcagtaaac ttatccattc catctttttt agactctttg ataattttag
     4021 atatcaattc gtcctcatct acaatgaact tttcatccat ttcatacatc aagtctaaaa
     4081 aatattgaat atcgtcatcc tcaaaaaaag attgataatc tgaagatata ttttttagaa
     4141 taaaagcgat ggcatcatcg tcatcgtaag ctgatagatt tttttctgat gacataagtc
     4201 taattttata ttgttactaa tatgcaaaag tacaaaataa acagtaactt tgccacttct
     4261 ttactataaa ataaaaagta aaacatgtct atacatcaaa ttgaagatca aagaaaagtc
     4321 actacacatc gtttgttaga aatgaaaatg cgtggtgaaa aaatcagtat gcttactgca
     4381 tacgattatt caatggcaac aataatcgat gaagccggaa tggatgttat tttggttgga
     4441 gattctgcat ctaacgttat ttgtggaaac gtaaccactc taccgattac attaaatgaa
     4501 atgatatttt tagcaagagg cgttgctcgt gctgcaaaaa gagcattagt tgttgtggat
     4561 atgccttttg gaacttgttc cggtaatcct agaatgtcgc ttgaagctgc aattaggatt
     4621 atgaaagaaa caggtgcaga ttcattaaaa attgaaggtg gtaaggaagt tgtcgaagat
     4681 attaaaaaaa ttattgatgc aggtattcct gttatgggtc atcttggatt aatgcctcaa
     4741 tctataaata aatatggtac atacactgta agagcaaaag aaaaggcaga agctgataaa
     4801 cttcttgaag atgccaaact tcttgaagaa gccggttgtt atggcatagt actagaaaaa
     4861 attcctgcca aacttgctca aaaagttgca cagtcattaa gtatacctgt tattggtata
     4921 ggtgcaggtg gttatgttga cggacaagtt ttagtaatgc acgatatgtt gggtatctca
     4981 caaggttttt caccaagatt ccttcgtagg tatgctgata ttaacgggat tgcaacagca
     5041 gctgttcagc aatacataaa agacgtaaaa agtaaagatt ttccgaacga aagcgaacaa
     5101 tattagaaac gatacattaa atatatatac aaaaaaatgc ttaataactt tgattattaa
     5161 gcattttttt acgagcgtta tcgtttaata aatttctcaa ttttgccatt taggcgcaca
     5221 taataaacac catctgataa ggtagaaata tcaatgttag caatagtttc atcaaatata
     5281 tttttactta cgatacgacc tgacatatca tatataataa gctccgtttg cgttaaataa
     5341 tctgatgtaa ttgttatttg acttgttgca ggattaggga atatagagaa tgaagtttcg
     5401 ttaatttctg aaacttcagt tgcacacaag tcgaagtttc cttctttatc gttcatataa
     5461 accgctcctt caccgcaaat aaaactatta ccttctattg attttgaaag cccggtcaca
     5521 tttaatgttg catttaccat aatatcagga ctttctgcag aaccttttcc tttctcaata
     5581 atcaactcac taatagtaac attagtgtta ataattggac gcaccattcc attatcaggt
     5641 ataacaacaa tagaagaagt tgtcggaacc attccttgtt cccagttagc acaatctgtc
     5701 caatctgtag tacccttatt tcctgtccat atacttgcac gagtatcagc aacatctgac
     5761 attgtttcag taacaacagc aggtgcctcg tatgtagctt taacccacgc cttttgagca
     5821 atattattcg tactgctatt ccatatttta attgcaatcc aacctacttg ggttggtgta
     5881 tatgtcaatg taacatcttg cgtgccattt gcttttgata agacattacc atctaaatca
     5941 taaattgcta aagttatatc attgccttca tcggccggat gcatcacaat acttaattct
     6001 ttgtcatttt cagcataaat ttttcctact aaacgttgtg ctgtagaatt ttcaggtaat
     6061 gcacctcctt gaccaagtga ttcacagtga ctatcgccta aatcgttagc catttcccac
     6121 tcttgaacag ttataggcga tcgatatggt gtataattcg tttctttgtt tgcaggggcc
     6181 catactgaat aacccaaacc ggtaccggta ttagtaactt tagcacagaa tcttccgtct
     6241 gaataagcag tacgattacc tgatactcct cctgaataat ctttaagctc aacatttctg
     6301 aagtctgtat caatccattc ttccctatcg cttgctccat tatcagttat agcaattatc
     6361 gccttgcctg cgcgttccac aattaataaa tcgccatctt gccaacgtac tttataatcg
     6421 ccatatttaa aatcaagatt ttgatgacac catattaaat tcttaactgc atcacgcaca
     6481 ggtaattctt cttcattttt aggatcgtga gtccaacgtt taccattcga taaattaaac
     6541 aaatcttcca tgtaaacact aatgttcccg tcgagtgcca tggctattgc ataagccata
     6601 cctaaacgtt tatcgaatgg atcaatatgt cctccggttt cgttatttcc atcccatcct
     6661 atatattttc catcttcatc aaattcagga cgaaatgtat catgactatt tacataattc
     6721 aacgaacgat gtaccttttg agtcgcataa ttttcatacc gcatagtaag ttgcgaacct
     6781 ggtacatctg acaaatttgc gtatcctcct ccacttacta tatttttaat agttccgcgc
     6841 aatggcacat cgtaagcgcc catcaagaat tcagaaccgc catttgcata ttttacatca
     6901 ttgcaatatt gatctattct gctttttcca tcaataaatt cgcctacatt aaacatcgct
     6961 tcaccacccg cacaccagct tggcaaagaa tatttcacat tgtacacaaa atcttgttgc
     7021 gcccattcag ggaagtgttt tactgcatcc catctgtaac catcaacagc tgtttgtttt
     7081 gtaaaccata taagccaatc tcttgcttga tttcgcatat aatttgcaga ttgtgtcggg
     7141 ttgtatccaa cggcatttga actttgtccg tatgcacctt cgtaataaca aatatcgggc
     7201 ccccaccaat cagaacacca atcaccggta ttgcaatcat gtgcttgatt tggatggaaa
     7261 ttaggccaat tttttgccca tcgtccgcta cggctccaat aatcgctttc agaaccatct
     7321 gtagcaggtg tatcgtatga tacgtatctg aaattcttcc atttattatc tctcgcaccc
     7381 gggtcttctc caccatttcc atctttttca gaacccgctc cgtcaacgtg atttaaaacg
     7441 gcatcttcaa taacctctat tccatttgcg tgcatcacag ctatcattct caacacctca
     7501 tctttagtac caaaacgagt agtatttgat cctttctgat acttatctcc caaatcataa
     7561 tggtcaaatg gagaatatcc tacagaacct gaatgtgggt ttttgaaaaa tggaggtacc
     7621 caaaccgcat tgatgtcgag ttcttttaaa cgcggagcta attcggtcaa ataatttgca
     7681 tacgaattgg gataatcatt atgccaataa tcccaccaga atgcttgtag aacgaccttt
     7741 ttttcaacgt cagcagaaag aggtgctgaa caaaagagag ctaataaaaa taaaaaacat
     7801 atttttttag ctcgaaaata cattttttcc atgaatttga gttttagggt taggtttggc
     7861 aaatatacaa atatttatta gaaaagcaat gattacattt atcatcttat tgtttaaaaa
     7921 attattcgtt ttttagctcg tttttctata cctttgcgaa gtaattttgc atcaaatatg
     7981 aaacaatact tactattaat attcgctttt acatgggctt tactgcctct atcggcacaa
     8041 atagaccaag gagcaatgct cgatgccata aatgaactcc gtgcagaagg ttgtacttgt
     8101 ggtacctcag aactaaagcc tgttacaagt gtaagctgga ataaagactt agctgaaatt
     8161 gcaaagtctt actcagacca cttaaaagac aacaataaaa gttcaaaaaa caatttccta
     8221 tttttaagcc atgtaggtat agatggcagt acacttgaaa gtagattggc agaagcaaac
     8281 ttcaatacaa aatttgcatg cgaaaatata gcatttatcg aaggcaacga agatacagct
     8341 attgactatt ggttgaataa tcctgaatcg tgtcaaaaca ttatgagtaa acaatctact
     8401 gctttaggag ccgcacgtag tggtgacttc tgggtactga ttttagctca acctataatt
     8461 aaatcgaaaa aagacaaaac tcaagaagat ataacagaat aatatcaaat ttttaatact
     8521 aaatcattat gcaaactatt gacaattaca aatttgctgg aaagaaagca attgtccgcg
     8581 ttgactttaa cgtaccacta gacgaaaatg gaaacatcac agacgacact cgtatacgtg
     8641 gtgctttacc tacattgaaa aaagttttga acgatggagg tagccttatt ataatgagtc
     8701 atatgggcaa accaaaaggc aaagttgcgg ctaaatattc attgaaacaa atcttagatt
     8761 cagtatccaa aacattgggt acagcagtaa aatttgctcc tgattgtgca aaagcatcac
     8821 aagctgctgc tgaattgaaa gcaggcgaag ttttactatt ggaaaactta cgtttctatg
     8881 ctgaagaaga aggcaaaccg actgatatag aaaaagaaga tcctcgctac gaagaagcta
     8941 aaaaagcaat gaaagcatca caaaaagaat ttgctaagac tttagcatct tatgctgatt
     9001 gctatatcaa cgatgcattt ggaacagcac accgtaaaca cgcatcaaca gctgttatcg
     9061 ctgatttctt cggaactgac gacaaaatgt taggttattt gatggagaaa gaagtaaatg
     9121 cagttgataa tattttaagt aatatcaagc gcccatttac tgctattatg ggtggttcaa
     9181 aagtttctac caaaataggt attattgaaa acttaatgga caaagttgac aatcttatct
     9241 tatgcggtgg tatgacatat acttttgcaa aagcattggg tggcgaagtt ggtaaatcaa
     9301 tctgcgaaaa cgacaaatta gatttggctc ttgacatcat gaagaaagca aaagaaaaag
     9361 gagttaactt ggtattagga actgattgtg ttgctgcaga cagcttcagc aatgatgcaa
     9421 aaacaaaaat tgttccatca aacgccatac ctgaagaatg ggaaggcttg gatgcaggtc
     9481 ctgaaacacg taaagcattt gccgatgcta ttgaagatgc aaaaacaatt ctttggaacg
     9541 gtcctgcagg agtgtttgaa tttgataatt tcaccggagg ttctcgtgct atagctgatg
     9601 ctatcgcaaa agcgactgca aagggtgcat tctctcttat cggtggtggt gattctgttg
     9661 catgtatcaa taaatttggt atggcagacc aagtatcata tatctcaacc ggtggtggag
     9721 ctttattgga agctatcgaa ggtaaggtat taccgggtat cgctgcaatc aaaggataaa
     9781 attttataga gttctgattt tttagaactc taagcataat tgggggatgc tattacaaaa
     9841 aggcatcccc ctttattgtt agttaggcaa tattacaact atggatacaa aagaagacaa
     9901 aaaaatattc cattcgtgta tatgattttt aattgtgtat cagttttttt agctatttct
     9961 attggaaaat tatcagacaa aataggaaaa gatttaaaag gtaccggatt cggattgtat
    10021 catgctatat tgggaatcac cttactccct gccagtttaa ttgccggact tctatacgat
    10081 aatgttaatt ctgatgctcc attttatttt ggttctttaa tggctatgct ttctgtaatt
    10141 cttatgatta tatttgtaat ttcaaaacct aagaaacaga ttattaataa ggactaatta
    10201 gtgataaaat atggatgcca gtaatattat tcggttttta aacgagctaa gagcaaataa
    10261 taacaaaaat tggttcgatg ctaatcgtaa atggtacgaa gatgtaaaat cggatttctt
    10321 tgaattttct aaaaaattaa ttatcgaagt atcaaaattt gattctcgca taaataattt
    10381 ggatataaag gattgcactt accgtattaa tcgagatata agatttagca acgacaaaag
    10441 tccatataaa actcatttcg gcacttttat atgcccgaaa ggaaaaaaat caggattcgg
    10501 aggatattat tttcatattg aaccgcaagg tggtgattat ttaggaaatc accttatggc
    10561 tgttggtgca tattgccccg agaaagatga actggaaagc atccgcactg aaatttacga
    10621 taatccagaa aaattcatac atgcacttaa gcaggcaaaa ggatttacat ttgatgaatc
    10681 tgcaaaactt aaaaagaatc ctaaatcata tcctgcagat ttcaaataca tagaatattt
    10741 aaaatacaaa aactactgca tcgaaaaagt tttatcggaa aaggatttac aagaccctaa
    10801 tttattggag atactacttg ataattataa atcggcacac gcgttcaatg aaattatcaa
    10861 caaatctata gaatatatta aagaaaatgc ctaaatggct tattacaaat cactccacat
    10921 aggaatctct tttataaaat catacgacat attgtcgtaa tccatcttac agcaataggt
    10981 ttgagattga tttgtaacta cgatataagg aacgtggaat tgactattat aacgcatcac
    11041 ttgttcgaat acagacttgt ctattattac ggaaggagct ttatactcga ctaacattat
    11101 cggcgtcatt tgtttgtaaa ctaatgtatc acaacgctgc tttaaaccat tgatttctac
    11161 aatacattcg ttattgatgc agacagccgg atagttcaga ctgtcaataa gatatgcaca
    11221 aaaaccttgc ctcacctttt cttcaggcgt agcagctacc catttcattc tgcatgcatc
    11281 aaaaatatag ggttttcctt taatattttt tactctgagt tccaaaaatt gattatcttc
    11341 gctgaagttt aaaactacat acaaagataa gaactttggc taaacaagaa gttacatacc
    11401 aagatatttt acagcaatta caactgcggc aattttctcc tatatacgta cttatgggag
    11461 aggagcccta ttacttagat aaattaactg attactttga gagcaatgtt ttgagtgaag
    11521 acgaaaggtt aatgaatcag gttgtatatt acggcaagga ttcacaagtt gaaacaatta
    11581 tcaacgaagc tatgcagttt ccgctcatgt cgccttatcg ccttgtcatt ttaaaagaag
    11641 ctcaaacact tgacaataag aataaattag acctgcttga acattatttg aaacaacctg
    11701 ccccatctac catacttata attacataca aatacggaac aattgacaga cgaaaaaaat
    11761 ggattcaaca agccgagaaa ttcggagtcg ttttggactc aaaaaaatta cgagattacc
    11821 aagttactac ttttattgaa tcgctggcaa gaagtgtaaa attaagtatt gatgaagaag
    11881 cagttgcaat gttaatcgaa tttattggta ctgatttaac acaattggaa agtgtaataa
    11941 acaaattcaa tattttactt acaactaatc gccgtattac taccgagata atagagaaaa
    12001 ctataggaat aagcaaagac tatacagcct ttgaactgat aaatgcactg atcaccaaag
    12061 acgtagtacg ttcaaatcgt attattatgc atactacatc tgcaatacag caaattatat
    12121 cggttttatt caattatttt tcaggcttaa tgatatatca ttatttgcaa aacaaaagca
    12181 aagataatgt agtagcgcaa ttacgtatta atccttattt tgtcaaagat tatgaactcg
    12241 gcgcgcgtaa ctttagtaag atgaaaacct ttaatactat aggatattta cgcgaatatg
    12301 atgccaaatc gaaaggagtt aataatatat cagccaatga tttggacttg cttaaagaat
    12361 tgatatataa aatattgcat tagaattaga atttgtattt cgaattacga gcatccaaga
    12421 atttatttat agtttatttt aatacaagga tattattatg gacatcaact catattcatc
    12481 gtattttttt gtcggaattg caggcacggg aatgagtgct attgcacaat atctaaatgg
    12541 tatcggaaaa caagtatccg gctctgacag actattctct actacagaaa aaatgttatc
    12601 tcaaacacaa tttgaagcac aaggtatcag ttgttatttt caagatggta gtggaataag
    12661 caatcaaaca gatgtcgtga ttgtatcaac agctattgaa gaatcaaaca ttgaatatca
    12721 gaaagcctta aaacttaaca ttcctattgt aaaacgttca gaactactgg ctgccatatc
    12781 tgatagtatc aaaacaatcg cagttggcgg tacttctggt aagtcgacaa ccacagctat
    12841 gatatttcat attttagaaa aatgcggaaa atcgccttca ctaattaccg gtgcaggttt
    12901 atccgactta caagacaaag gtctacccgg aaatgcatgg aatggtaagg gcgaatggtt
    12961 ggttatagaa tctgacgaat ctgacgggtc tattgtaaat tatcatccgg aaatcgcatt
    13021 gctccttaat attgatcgcg accataaaga gttcgaagaa ctaatcgatc tgttttcgat
    13081 atttaaatcg catacaaaat ctactttcat cgtaaaccaa gattttgaac taactcgaaa
    13141 attatctcaa gacatcaaaa atgacttcgg tacacaaaac aatgcaggca tcaccggtag
    13201 taattttgag caaaaaggat ttagcatcaa ttttcagatc gatggaaaga actgcgtcgt
    13261 accattaata ggtcgtcata atatggaaaa cgccttagcg gcaatagcag ctgcaaaagc
    13321 agttgggatt agtttgaatg atgccataaa tgccttagca gacttcaaag gcatttatag
    13381 acgtgctcaa ttagtaggtt cacgaaaaga tacaaatcag tatgtaatag acgattttgc
    13441 gcataatcct gctgaagttg cgtctgccat tagggcttgc caaactatag caccaaaagt
    13501 aattgcttat tttcagccac atggttttgg accacttagt tttatgcaca aggagcttgg
    13561 tgagatggtt gcaaaaacct tacgcaacga agatatattt ttaattggtg atgtttacta
    13621 tgcaggtggt actgtcgata aaaacatatc tcctgttatt gtaagtgatg caattaacaa
    13681 aaccggaaaa aacgcaaaat ttgttggaaa caaacagcaa gcacttaaag ctatattaga
    13741 agaattaaat aacaacacgg ttattttaac catgggcgca agagacccta aattagatgt
    13801 gtttgccaaa gaaatttttg aggcattata acaagaatcg aattatacaa aaatagccga
    13861 ccttatgggc cggctatttg tttttattta ttgtagatta aaatacagcc ttacatattt
    13921 tctttaggtt atctgattct cccaaagaat aaaaatgtaa actcggtaca ccaaatgcca
    13981 acaaatcctt tgcttgagct actgaccatt ctacgcctaa ctctcgaacc tgttctttag
    14041 ttttacactt ttgtatttct tttactaaat cctcaggcaa atcgatgtta aagatttgag
    14101 gtaacaaact aatatcatta agggctgcta ttggtttaat tcccggaact ataggaatat
    14161 taataccgga tgccctacat gatttcacaa agtccttata tttttgatta tcaaaaaaca
    14221 tctgtgttac tgcatagccg gctcccaaat ttaccttttc tttaagatat tgaatatctg
    14281 ttgccaagtt tggagcttca atatgttttt cgggatagca cgcaactccc atagaaaatt
    14341 gtgtggcctt tggatttttc aaagaatcat ccaagtactt agcactattc atcatattga
    14401 cttgctgcat caaagatgta gtatgatcat gtccatcttc ttttgggata aatatccttg
    14461 aacctttttg aggatctcct ctcaaaacaa atatattatt gatatccaag aaattaagat
    14521 caatcaatag attctctatt tcttctctat ttagtccgct acaaataata tgaggtacaa
    14581 cttcaatatt gtatttatac ttaatagcag ctgcagtagc taccgaaccc gggcgtttac
    14641 gcactaccat tttggagata cttccatcag gcatattttt atatactata tcggcagcat
    14701 ggcttgtaat atttatgtat gagggattaa attccaacaa attatcaata gtatcataaa
    14761 tagcatcaat gccacgacct tttaacggag gtaataattc gaaagtaaat aacgggtgat
    14821 ctgaattatt aagtttttct gcaatattca ttatcgtcct atatataagt tattcaaaaa
    14881 caatcacaaa gatatacaaa ttttaaaaat atttggcctt atcgcaagca aaatagaatc
    14941 cgcatacaga cgaaacaggt tgcatggcat ttgtctctgt gagtgttacc cctatcgagt
    15001 tagtaacatc gagcaattca aaaaactcac gtttcatact atgatcaggc aacgacggat
    15061 agcctattgc aggtcgtata ccatttttaa agccccacca ctcatctttc atcttcgtat
    15121 gcaagagttc tgaaaaagct tctactaatc tatctgcaag taattgcaac atcattgctt
    15181 tataatcatc tccactcttc tttagagttt ctaataaatc agttacattt atagttgctg
    15241 caaatagtcc tacataatcc agattcggag acagaaaatc agtcaaacaa acttcatcct
    15301 tacgaacaaa attaaactta tatccgttaa tttctatgct ttcattctct tttttcaccg
    15361 gagatattgc aacaacagca tttgctttta ttttgattat attttcattc aaaaaacatt
    15421 ttgcctcttc tatcaaactt tctgcatgtt cgccttttac cttccaagca gcacaaaagg
    15481 ctgtccaatt aatatatggg attatttcat caatgggata atctgtaagc acatttttac
    15541 ctacaaaagt aggaacatat actttttcag tcgtccaatc gatattaacg gcttttgaaa
    15601 ccgattttgt ttgcttattt ttaattttat cccgaagttc actatatttt gctttaatgc
    15661 ttgccttgta attatcatca ttaagtagtt tagatgctat tgacacattg gttgatgcat
    15721 ctttagaatg tacaacagct ccgtcatata aaggatctac ataatatgca gtatgctctt
    15781 ctgatgttgt tgcgccccct attatcaatg gaatatttaa tttattgatt tgcatctctt
    15841 ttgcaacttc ctccatttcg tgaagtgacg gtgttataag accacttaaa caaaccattg
    15901 acgcattatt ttctattgct gacttaataa taagttcttt agggaccata actcccaaat
    15961 ctataatatc atatccgtta caagaaagca ccacacttac aatattttta cctatatcat
    16021 gaacatcgcc tctaacggta gctatgacaa tcttctttct aatagttttt gcatcatctt
    16081 tctctatatg gggcataata tatgatacgg cttttttcat tacttgagca ctcttcacaa
    16141 cctgcggcaa gaacattttt ccctctccaa aatatgttcc tactttagta atacccttca
    16201 tcaatatgct ttcaatgact tctaaaggcg gatatgtatt caatgcttca ttaatatcag
    16261 actccacata ctctgttgtt ccgttaatca atgcatattc caaacgttct tcaacactta
    16321 aaatacgcca tgcatcagtc atttcttcct tttttgtgtt atcgttttgg aacgatgatg
    16381 cataagttat tagttcgtct gttgcgttag gatgagtgtt catcacaact gcttcaactt
    16441 tatctagtaa ttctttagga atattatcat aaacttccag catctccgga ttgactatgg
    16501 ccatatccat gcctgcatta atagcgtgat acaagaaaac agaatgcata gcttcacgta
    16561 ccttattatt gcctctaaat gagaacgata agttactgat gccaccgctt accttagcgt
    16621 atggtaaatt tttcttgata tattcgactg tgcgtataaa gtcaactgca taggaatcat
    16681 gttcaggcaa acctgtagca attgaaagca cattaggatc aaaaataata tcttctggct
    16741 taaaacctgc tttttgtacc aataaatcat aagccctttt acatactgat attctacgtt
    16801 cgtaagttga tgcctgacct tcttcatcaa atgccatgac aactactgct gcaccactat
    16861 ttttaatatg agtggcatgc gacaagaata cttcctcgcc ttctttcagc gaaatagagt
    16921 taatgataga ctttccttgt acgtgtttta aaccctcttc aagcacttcc catttagatg
    16981 aatctatcat tataggaacc ttagctatat aaggatctgc ccctaataga tgcaaaaatt
    17041 ttgccataca agttttagca tctaacatag aatcatccat acaaacgtca ataatactgg
    17101 ctccgccatc aacttgctta cgtgcaacta ataatgcttc ttcgtattta ttttcggcta
    17161 ttaatcgtgc aaacttttta gaccctgcaa catttgtacg ttcaccaata tttgtaaaat
    17221 gctcaatcgt caatggttct aagccactca atctagttaa tctagcttga ttttgcggaa
    17281 ttcttggaac attattatgt gccatatcag ctatcaatcg aatatgttcc ggagtagtac
    17341 cacaacagcc tccaactata ttcagaatgt ttgattcaaa atatggacga atctgctcta
    17401 tcattatctc ggcagtttgt tcgtattgac ctaattcatt tggtaaaccg gcatttggat
    17461 aggcactaat ataggcatct gttaattcgg ataaattttt aagatatggg aacatgtcat
    17521 ttgctccaaa tgaacaatta agacctattg ataatggagt aaacggagta actgctacaa
    17581 caaaagcctc taaagtttga ccacttaata atctgccatt cttatctatt gtagcagata
    17641 tcataagagg catttctttg tctactctat gcgcagcaca taaagcagcc ttaagattca
    17701 gtgtatcaaa acaagtttca aacaagaaag catcaacacc tgcatctttt aaagcttgta
    17761 tttgtatttc gtatgatgca gacatctcgt cgaaagttat atcatcagac attgtaagtg
    17821 ttttgtttgt aggaccaata gaacctgcga cataaatatt acgtttgtca cattttctag
    17881 cctcatcaat cgcaagactt gcgtatctaa gattaacatt tctaatatca gatccctcgt
    17941 ttgccgtaaa tgtattggtt gaaataatat cggcgcctgc ttctatgtac atacgatgta
    18001 t
//
LOCUS       DAOPID010000007        15055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_4537_length_15055_cov_4.602933, whole genome shotgun sequence.
ACCESSION   DAOPID010000007 DAOPID010000000
VERSION     DAOPID010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 15055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15055
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4537_length_15055_cov_4.602933"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1599)
                     /gene="feoB"
                     /locus_tag="PK641_00700"
     CDS             complement(<1..1599)
                     /gene="feoB"
                     /locus_tag="PK641_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HPG54908.1"
                     /translation="MKLSDLQTGKTGIIVKVLGSGNFRKRIIEMGFIRGKKVEVILNA
                     PLKDPIKYKVMDYEVSLRRNEASLVEVISEEEAKAQRKEHKDLPIGIEEPVQIMEHIA
                     IEKSKTINVALIGNPNSGKTSLFNIASGSHEHVGNYSGVTVDAKEGHFSFGGYKFNMF
                     DLPGTYSLSAYTPEEIYVRKHIFEKMPDIIINIVSASTLERNLYLTTQLIDMDIPMVI
                     ALNMYDELEKSGAKLDYKTLGKMLGVPIVPTIANEGFSGDSGLNNLFETIIKVYENKE
                     PTVRHIHINHGGDLQDAIDKLNILIKQAGNFESQVASRFFAIKLLEKDKEADKYLKTL
                     DNAKELYSFRDKVSPHIEEELQEDCESAIINAKYGFIAGALAETYLEGKKETFKLTRI
                     LDPIVTHKFWGYPIFFLFMWIMFECTFSLGAYPQEWIESGVSLLGNWIANIMSDSPLK
                     DLIVDGIIGGVGGVIVFLPNILILYFFISLMEDSGYLSRAAFIMDKIMHKMGLHGKSF
                     VPMLMGFGCNVPAIMATRTLESKNS"
     gene            complement(1630..2754)
                     /locus_tag="PK641_00705"
     CDS             complement(1630..2754)
                     /locus_tag="PK641_00705"
                     /inference="COORDINATES: protein motif:HMM:NF016830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HPG54909.1"
                     /translation="MKKVFRVAIICKFILITTHIANAYEAGPFTLEAMYIGDVIGNLH
                     GGIKTGAAYMGKADIAVSFHTDRAKWWKGGILKVKLENTHGGTPSADLVGDFQVFNNI
                     EAGNHTYLSELWYAQQIKRVRLQLGLIDMNSDFMVTDHAGIFMNSSFGVPSTISTNNP
                     IPIFPLTGLGFTIQYAVTDGLQLAGGVFDGTQKDWDENPYNVNWSFSKYDGIFSIFEV
                     QSTYSIFKSLESNIKLGAYNWWHVDQLSNADNYNYGLYLSLEQEVFHTSSDACMHVFS
                     QLSWTPAAYNYNDVYFSVGAHATGFWKKRPADEAAIAMCMAYFSSDKEAETNLEVSYK
                     FSFLNHIYMQPHFQCIFNPKGTETHLDTAILLGLRFGLNF"
     gene            complement(2885..3820)
                     /locus_tag="PK641_00710"
     CDS             complement(2885..3820)
                     /locus_tag="PK641_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HPG54910.1"
                     /translation="MAYKEDTHISDIIAGNFNQVLLMEHLGMPLVVQRKTVKEFCTEN
                     NIKADLYLAFVALYDGMQVVQIPNFDKNDINCILTYLKNCHNYYLNEKIPQIKNYVKQ
                     ISVSNNSSGIKLLNDFVNSYIEELTEHFEYENNIVFPYITALSQNQTLDSSYSVTIYK
                     QHHNNIDESLSSVKELLIKYLNFTDEQNIRRKMLACLFELEYDLKIHSHIEDNILIPL
                     VEKYENIEHVQKDKSENVLSQREIDVLKYLIEGLSNKEVAEKLYLSTHTVISHRKNIC
                     EKTRIKSLAGLTIYAILNGIINIDSLEEKQNQNAH"
     gene            complement(4000..4569)
                     /locus_tag="PK641_00715"
     CDS             complement(4000..4569)
                     /locus_tag="PK641_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG54911.1"
                     /translation="MRLKSLLIALLISICNINAQEFTEWSTTSYESGITPDCSQTSPE
                     YSDSLNNFLRIKVGANTDVIVRLMRIGEPKDVCIRSVYVKSNESYEMRQIPEDVYYIK
                     IAYGKDYREKNDSTGCIGKFFINQHFEKSKDLMDFTRAKQADRIIDGKKHEVWHLPTY
                     EIFLDASELMLDDKKTMNNNRISEQEFNK"
     gene            complement(4743..5828)
                     /locus_tag="PK641_00720"
     CDS             complement(4743..5828)
                     /locus_tag="PK641_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG54912.1"
                     /translation="MKRTGFILLYLLITLISVDAKLPVKFLYDLDFLFYFDNREYDGY
                     PYQRSQTLFGARLSPEIGVGISDSIVGDHKLMAGASYIQPIGADYRDAKILPTLYYQY
                     KKSGFSVHLGLLPFYKRIETLPDYLMYDSIAYYHPNIQGALFQYESKQGFVELMCDWR
                     GMWSSTTREAFRIVLNGRYEYKVLFVGGFAQMNHLANYAPPAPHIGVCDDILINPYIG
                     VNLGNVTPLDSFSIRAGYLLGIQRDREAGLNYIPQGGNLEIFLRWRMLGLKNTTYYGE
                     SQNPLYPTQKSLLNQSDPFYVARFYNRTEVFVYIFNNSFVNCYLSANFHVADGTFSTQ
                     QQVIAQFRLDGIKNYKQKKKLKSIFGK"
     gene            complement(6080..7747)
                     /gene="asnB"
                     /locus_tag="PK641_00725"
     CDS             complement(6080..7747)
                     /gene="asnB"
                     /locus_tag="PK641_00725"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HPG54913.1"
                     /translation="MCGIVCAFEIKQPVPELRTQVLKMSKKIRHRGPDWSGIYSNENA
                     ILAHERLSIVDPISGKQPLYSKDGKLVLAVNGEIYNHQDIRKQFAGQYEFLTKSDCEV
                     ILALYSKKGPNFMEDLSGIFAFALYDVEKNVYMIGRDHIGIIPLYQGWDKDGVYYVAS
                     ELKALEGQCCKIEEFLPGQYLYSPDKKPKIWYKREWTNYESVKNNNSSIDMLKSALED
                     AVSRQLMTDVPYGVLLSGGLDSSIISAIAKKYAAKRIESGNVSDAWWPQLHSFAVGLV
                     GSPDLAAARKVAEHIGTIHHEVNFTVQEGLDTISDVIYHIETYDVTTVRASTPMYLLA
                     RVIKSMGVKMVLSGEGADEIFGGYLYFHKAPNAEEFHKETVLKIGMLHMYDCLRANKS
                     LAAWGVEGRVPFLDKEFLDFAMNLNPKDKMATDGKMEKWILRKAFEDYLPESITWRQK
                     EQFSDGVGYSWIDTLREMTSKLVSDEQMANAAEKFPINPPMTKEEYYYRTIFAELFPS
                     ESAAKCVPSLPSVACSTPTAIEWDAAFKQMIDPSGRAVKSVHQNKYE"
     gene            complement(7795..9219)
                     /locus_tag="PK641_00730"
     CDS             complement(7795..9219)
                     /locus_tag="PK641_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HPG54914.1"
                     /translation="MGNPKAFLSIDRKEAGYRPINERKYDFGEVEQTLNSEDRKLQAS
                     RCMDCGVPFCHWACPLGNINPEWQNLLYKGLIREAAELLQSTNNFPEFTGRICPALCE
                     NACVLNHSINLAVTCRENEAAVIEHAFNEGYIIPMPPKKRTGKNIAVIGGGPSGMAAA
                     DQLNKSGHTVVLFEKNEAVGGLLRFGIPDFKLNKKVIDRRLNILKAEGITFKTGVKVG
                     IDYPTEKIFSNFDAICLCLGAEQARDLPIEGRDLEGVHFALELLQQQNRIISGKHIDS
                     NNFLTAHGKRVLVIGGGDTGSDCVGTSIRQGAKSVTQIEIMPMPPERENSATPWPNWP
                     NILRTSSSQQEGCERRWCLTTNKFIGKNGKLTAVEVEEVEWLPSPDGGRAIMNKTGKI
                     ETLEVELVLLSMGFTQPKHEGLLNEMGLKYDERGNVATNANQQTSIPKVFAAGDVNTG
                     SSLVVRAIAAGRKAAAEIDKFLIK"
     gene            complement(9241..13749)
                     /gene="gltB"
                     /locus_tag="PK641_00735"
     CDS             complement(9241..13749)
                     /gene="gltB"
                     /locus_tag="PK641_00735"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HPG54915.1"
                     /translation="MDTKGHDLYRPEFEHDACGVGLIAHVHGAKTHDIVEKGLQILEN
                     MLHRGAESADNKTGDGAGILTQIPHEFILLQGIPVPEKGTYGTGLVFFPKEKDETAVC
                     INIINTIVENEGLKMLAVRDVPTNSDILGEISRSNEPNIKQIFITGEADQDKLELKLY
                     VIRKKIEKVALKSNLSQKRNFYIVSLSSKRIVYKGMLTSLQLREYFPDLTSPYYTSGL
                     ALVHSRFSTNTFPTWDLAQPFRLLGHNGEINTIKGNRFWMEARESIMQCDKLGNMDDM
                     YPIVQPYMSDSASLDNVLEFLLMTGKSIPHALSMLVPESWNDKNPISNCLKSFYEYHS
                     IIMEPWDGPAAILFSDGRYAGGMLDRNGLRPARYTITKKDTIIIASEAGVLEIDPDDI
                     KEKGRLKPGKILLIDTEKGEIYSDALLKDELAKAYPYSEWLSKNRIELDKISTGRTAK
                     YEVENYYRLLRAFGYYKEDFEKLIIPMVQDGKEPTSSMGNDTPLAVLSNKPQRLFSYF
                     RQLFAQVTNPPIDPIREELVMSLRLYIGAQEGNLLVPSPKLCKMVKLESPILSNRQFD
                     ILQNLIYKGFKTLTLPMLFEPEKGVNALESALDELCQKSEQAVIDEYNYIILSDRGVD
                     KKHVAIPSLLAVSAVHHYLIEKRKRMQIAIVVESAEPREIMHFALLFGYGATAVNPYM
                     VFASIDKLVKDNELQLDLHTAEKNYIKAVNKGLLKVMSKMGISTIRSYRGAQIFEALG
                     ISQSVIEKYFRGTISEIGGIDIHDIQDEAINAHQLGFGDNFNESKLMDNLGLYAYRKT
                     GEFHAWNPETISMLQIATRLGSFKKFKEYTDSIDFKQEPIFLRNMIQYKSDRKPIDIS
                     NVEPASEITKRFVTGAMSFGSISKEAHEAIAIAMNTIGGKSNTGEGGEDPARFKISKD
                     GLNRRSAIKQIASGRFGVTAEYLVNADELQIKIAQGAKPGEGGQLPGFKVDKIIAQTR
                     HSIPGISLISPPPHHDIYSIEDLAQLIFDLKNINPSARISVKLVSEAGVGTIAAGVAK
                     AKADMILISGGEGGTGASPASSIKYAGLPIELGLAEAQKTLVMNNLRGQVRIQADGQL
                     KTGKDIITAALLGAEEFGFASSALIVLGCVMMRKCHLNTCPMGIATQSEELRKRFIGR
                     YEYLVNYFNFLAEEVREHLAAMGYTKLEDIVGHSELLEFVPTQVNEKAKKINLKKLIR
                     LPKEAEKNDFHMVKEQNHKIENVLDIRLIAEAKSAIESHLSVDKTFKVRNTDRTVGAM
                     LSGEIARRYGDAGLAKNTIRYTFEGSAGQSFGSFLTHGISFRLEGESNDYLGKGLSGG
                     IISLVPAKNSNFEPEKNIIAGNTSLYGATAGEVYINGCVGERFCVRNSGAIAVVEGVG
                     DHCCEYMTGGRTVVLGPTGRNFAAGMSGGIAYVLDIEGNFDYFCNMEMVELSLIEDNS
                     DKIELQSLIKNHYKLTNSPLAKRILDNWDEYLRHFIKITPIEYKKVLHDEKIEAIKKK
                     IAQVEYDY"
     gene            complement(14020..>15055)
                     /locus_tag="PK641_00740"
     CDS             complement(14020..>15055)
                     /locus_tag="PK641_00740"
                     /inference="COORDINATES: protein motif:HMM:NF020260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG54916.1"
                     /translation="AVSIAYEKILPHDQKASYFIYFDVDPSKIDVNIHPTKTEVKFED
                     ESSIFPIITAVIKEALGKANVIPSINFDQDDAPSIPILNSDIEHVAPPKISYNPQYNP
                     FANTNSESSYSSKKNIDSWDKLYEINHLDHKIETKIETSDDDDRQYSLDYKENANTKF
                     SGFQLFGKYLIQSDVNGIKITNHQRAHIRILYENFVSAMTKNHSASQKLLFPEILELS
                     TIENSMFSEIREDLCSIGFIIEDFGKNAYQIIGVPSQMEDCNPIQLIHDTITAANESP
                     NALAEDLHKTIALDMAKHISTRKGNTISTEDASYIMNSLKQCSDQVYTPDGKRISYVL
                     TEQEINKHLQ"
BASE COUNT         4417 a         2845 c         2440 g         5353 t
ORIGIN      
        1 gctattttta ctttcaagtg ttcttgttgc cattattgcc ggaacattac atccgaatcc
       61 cataagcata ggtacaaaag atttaccatg taagcccatt ttatgcatta ttttatccat
      121 gataaatgct gctcgcgaaa ggtatcccga atcctccatt agtgatatga agaagtacag
      181 aataagaata tttggcaaaa atactataac tccgccaacg cctccaataa taccatcaac
      241 gatgaggtct ttgagcggac tatcgctcat tatatttgcg atccaattgc ctaaaagcga
      301 gactcccgat tcaatccatt cttgcggata tgctccaagc gaaaaagtgc actcaaacat
      361 aatccacata aagaggaaga aaatagggta accccagaat ttatgagtta ctatagggtc
      421 gagtatgcgt gtaagtttaa atgtttcttt tttaccttca aggtatgtct ctgccaatgc
      481 gccggcaata aatccgtatt ttgcatttat aattgctgat tcacaatctt cttgaagctc
      541 ttcttctata tgtgggctta ctttatctct aaaactataa agctcttttg cattgtcgag
      601 cgtttttaga tatttatcag cttctttgtc tttttccaat aatttaatgg caaagaatct
      661 actcgcaacc tgactttcaa agtttccggc ttgtttaata agtatattta acttgtcaat
      721 tgcatcttgt aagtcgccgc catgattaat atgtatatgt cgtactgttg gctctttatt
      781 ttcatatacc ttaataatag tttcgaataa attatttaag ccactatcgc cactaaaacc
      841 ctcgttggct atggtaggaa caataggtac gccaagcatt tttcctagag tcttataatc
      901 gagttttgca ccactttttt ctaattcatc atacatattt aaggctatta ccattggaat
      961 atccatatca ataagttgag tcgttagata taaattgcgt tctaatgtag atgcagatac
     1021 aatgttgata ataatatcag gcatcttttc aaatatatgt ttgcgaacat atatttcttc
     1081 cggtgtatag gcagatagag aatatgttcc gggtaaatcg aacatattaa atttgtatcc
     1141 tccaaacgaa aaatgtcctt cttttgcatc taccgtaact ccactgtaat tacctacatg
     1201 ttcatgcgag cctgatgcga tattaaacaa tgatgtttta ccggaattcg gattaccaat
     1261 taaggcaaca ttaattgtct tactcttctc tattgctata tgttccatta tttgaacagg
     1321 ttcttctatg ccaataggaa gatctttatg ctcttttctc tgtgcttttg cctcttcttc
     1381 acttatgacc tctactaatg atgcctcatt acgtcttaat gatacctcat agtccatcac
     1441 cttatattta atagggtcct ttagtggagc atttagtata acctctactt tcttgccccg
     1501 aatgaacccc atttcaataa tacgttttct gaaatttccg gaaccaagta ctttgactat
     1561 aattccggtc tttcctgttt gtaagtctga tagtttcata tattgtttat tttattttct
     1621 ctaatgttat taaaaattca agccaaaacg cagtcctaaa agaatagctg tgtctaaatg
     1681 tgtttctgtc cctttggggt taaatataca ttgaaagtgc ggttgcatgt aaatgtgatt
     1741 tagaaaacta aatttatagc taacttccag attagtttcg gcttctttgt ctgacgaaaa
     1801 atatgccatg cacatagcaa ttgcagcttc atcggcaggt cgtttcttcc agaatccagt
     1861 ggcatgagct cctacgctga agtatacatc attataattg taagcggctg gtgtccatga
     1921 taattgcgaa aatacgtgca tgcaggcatc agatgatgtg tgaaaaactt cttgttctaa
     1981 acttaaatac aaaccataat tgtagttatc tgcattgctt agttggtcaa catgccacca
     2041 attgtaggct cctaatttaa tattggattc taagcttttg aaaatactat atgtagattg
     2101 aacttcaaaa atgctaaata taccatcgta tttgctgaat gaccaattaa cattgtatgg
     2161 attttcatcc caatcttttt gagtaccgtc aaatacgcca ccggctaact gaaggccgtc
     2221 tgttacagca tattgtattg taaaacctaa accagtcaat ggaaatatag gaatagggtt
     2281 gtttgtggaa attgttgaag gtactccaaa tgaactattc ataaatatgc ctgcatggtc
     2341 agttaccata aaatcgctat tcatgtcgat aagtcctaat tgtagtcgga ctcgttttat
     2401 ttgttgagcg taccataatt cagatagata tgtatgattt ccggcttcaa tattattaaa
     2461 tacctgaaag tcgccaacca aatcggcact aggtgttcct ccgtgtgtgt tttcaagttt
     2521 tacttttaga atgcctccct tccaccattt tgctcggtca gtgtgaaaac taactgctat
     2581 atcagccttg cccatgtagg cagctcctgt ttttatacct ccgtgtaaat tacctattac
     2641 atctccaata tacattgctt ctagcgtaaa tggccctgct tcgtaagcat ttgcaatgtg
     2701 tgtagttatc aatataaatt tgcagataat cgcaactcta aatacttttt tcatttgtct
     2761 aaaattattt attgatagat ttgttttaca ctgcaaattt atgggtgttt aaaaaaaact
     2821 acaatcgcaa taatctgtca aatttcagga tggtatatcc cataaagaag ggattttatt
     2881 tatattaatg tgcgttttga ttttgctttt cttccaagct atctatattg ataatgccat
     2941 ttaagatagc gtatattgta agtcctgcca atgattttat tcttgtcttt tcgcagatat
     3001 tttttcgatg tgatataact gtgtgggttg ataaatataa tttttcagca acttctttgt
     3061 ttgataatcc ttcaataaga tattttagta cgtcaatttc acgttgtgac aatacatttt
     3121 cacttttgtc tttttgtacg tgctctatgt tttcatactt ttcaactaac ggaattaata
     3181 tattgtcttc aatatgtgaa tgaattttta ggtcgtattc taattcgaac aaacaagcaa
     3241 gcatctttcg tctaatattc tgctcatccg taaagttcaa gtatttgatt agtaactcct
     3301 taacactgga taaactttca tcaatattat tatggtgttg cttgtaaatt gtgacactat
     3361 aagaagagtc aagtgtttgg ttttgtgata atgctgttat ataagggaat acaatattat
     3421 tttcgtattc aaaatgttca gtaagttctt caatatagct gtttacaaaa tcattaagta
     3481 gtttgatacc cgaactatta tttgatacag aaatctgttt tacatagttt tttatctgag
     3541 gtatcttttc gtttaaataa tagttgtggc aatttttaag ataagtaagg atgcagttga
     3601 tgtcgttttt gtcgaaattt ggtatttgaa caacttgcat accatcgtaa agtgctacaa
     3661 aggcaaggta caaatcggcc ttgatattat tttcagtgca aaactctttt accgtttttc
     3721 tttgcactac aagtggcatc cccaagtgtt ccatcaataa tacttggttg aaattgccgg
     3781 caataatgtc ggaaatatgc gtatcttcct tatatgccat aattttatgt ggtaataacg
     3841 tatatttacc ttgcaaatat acggtattta gtagatatga caatccctta ttttcgggat
     3901 tttaattcca ttctattaaa tgctgtaaat cgagattatt tatacaaagt gtatatttat
     3961 tttttgctcg taaatatatt agaagttttc agaataatat tatttattga attcttgttc
     4021 tgaaatacga ttattgttca tcgttttttt atcatcaagc ataagttccg aagcatctaa
     4081 gaaaatctcg tatgttggga gatgccacac ttcatgtttt tttccatcaa taattctatc
     4141 agcttgtttt gcgcgagtga aatccattag gtctttgctc ttttcaaagt gttggttgat
     4201 gaagaatttg ccaatgcatc ctgtgctatc gtttttttct ctatagtcct tgccgtatgc
     4261 tattttaatg tagtaaacat cctcaggaat ttgtctcatt tcatagcttt cgttactttt
     4321 tacatagaca gagcgaatgc atacgtcttt cggttcgcct attcgcatca atcttacaat
     4381 tacatctgta tttgcaccta ctttaattcg cagaaaatta tttaaactat ctgaatattc
     4441 agggcttgtc tgagaacaat cgggagtaat tcctgattcg tacgaagtag tgctccattc
     4501 tgtaaactct tgtgcattaa tgttgcatat tgagattaat agtgcaatta ataatgattt
     4561 aagacgcatt ttatattgat aattaagttt aaataatatc tctgtcggtt attttgatac
     4621 acagacaaaa atagttaata ttatcaataa atatattcat cttcctgtat taaaggaatt
     4681 tttttgaaca tttttaaagt gtcattgata acataacgca agtttagagc gatgtagtat
     4741 gtttatttgc cgaatataga tttcagtttc tttttttgct tgtaattctt tatgccatca
     4801 agtctaaatt gtgcaattac ttgctgttgt gtgctgaagg taccgtcagc tacatgaaaa
     4861 ttggctgaca aatagcaatt tacgaaacta ttattgaaga tgtatacaaa tacttctgta
     4921 cgattataaa atcgcgcaac ataaaatggg tcactctggt tcagaagtga tttttgtgtt
     4981 gggtacaaag ggttttgtga ttctccataa tatgtagtat tctttaaccc aagcatgcgc
     5041 catcttaaaa atatctctaa atttcctcct tgtggtatgt aatttaatcc ggcttctcta
     5101 tctctttgaa tgccaagtaa gtatccggct cttatcgaaa atgaatcgag aggggttacg
     5161 tttcctaagt ttactcctat atacgggtta attaaaatat catcgcaaac acctatgtga
     5221 ggagccggag gtgcataatt tgctaagtga ttcatctgtg caaatccacc tacaaaaagt
     5281 actttgtatt cgtatcttcc attaagtaca attctaaaag cctcgcgtgt tgttgagctc
     5341 cacattcctc gccaatcgca cattaattcg acaaatcctt gctttgattc atactgaaag
     5401 agtgcgcctt gtatatttgg atggtaatat gctatagagt cgtacataag gtaatcaggc
     5461 aaagtctcga tccttttata gaatgggagt aatccgagat gcactgaaaa gcctgatttt
     5521 ttatattgat aataaagtgt tggtaaaatt ttagcatctc tgtaatcggc tccaattggt
     5581 tgtatgtaac ttgctccagc cataagttta tggtcaccaa ctatgctatc ggatatgcca
     5641 actccgattt cgggtgatag cctagctcca aataatgttt gacttcgttg ataagggtat
     5701 ccgtcgtatt ctctgttgtc gaagtaaaat aggaagtcta ggtcgtacaa aaattttaca
     5761 ggtaatttcg catcaaccga gattaatgtt attagcagat atagtaatat aaaacctgtt
     5821 cgtttcatag ggtacaaaga tagtgcaagc cgagagcaaa tgaaaattta ttttcgtttg
     5881 ccgaggcgca gcctatcttc atgaaaacca gattaaagat agtgcaagcc gagagcaaat
     5941 gaaaatttat tttcgtttgc cgaggcgcag cctatcttca tgaaaaccag attaaagata
     6001 gtgcaagccg agtgcaaatg aaaatttatt ttcgtttgcc gaggcgcagc ctatcttcaa
     6061 gaaaaatata ccaaattact tactcgtatt tgttctggtg aacactctta actgcgcgtc
     6121 cggatggatc aatcatttgt ttaaatgctg catcccattc aattgcagta ggtgtactgc
     6181 aagctactga tggtaatgat ggaacgcatt ttgcagccga ttctgatggg aataattcag
     6241 caaagattgt tctatagtag tattcctctt tagtcatagg tggattaatc gggaattttt
     6301 ctgcagcatt cgccatttgt tcgtctgata ctagttttga tgtcatttcg cgcaaggtat
     6361 cgatccaact ataaccgaca ccatctgaga attgctcttt ttgacgccac gtaatacttt
     6421 caggcaagta atcttcaaat gctttacgta aaatccattt ttccatttta ccatctgtag
     6481 ccattttgtc tttaggattc aaattcattg caaaatctaa aaattcttta tccaaaaaag
     6541 gaacacgtcc ttcaacaccc cacgccgcta atgatttatt tgcacgtaag cagtcataca
     6601 tatgcaacat acctatttta agaacggttt ctttgtgaaa ttcttcggca ttgggagctt
     6661 tatgaaaata aaggtatccg ccaaaaattt catctgcacc ttcgccggat agtaccatct
     6721 taactcccat agattttata acacgtgcta ataaatacat tggggtagaa gctcttactg
     6781 tagttacatc ataagtctct atatgatata ttacgtcgga tatagtatct agtccttctt
     6841 gcacggtaaa attgacttcg tggtgtattg tgcctatatg ttccgcaact tttcgcgctg
     6901 cagctaagtc aggagaacct acaagtccaa cagcaaaaga gtgtagttgt ggccaccatg
     6961 catctgatac attacccgac tcaattcgtt ttgcagcata tttctttgcg atagccgaaa
     7021 ttatagaaga atccaatccg cccgaaagta acactccata aggaacgtca gtcattaatt
     7081 gacgcgatac cgcatcttct aatgcggatt ttagcatgtc tattgaagaa ttattgttct
     7141 taacggattc gtaattagtc cactcacgtt tataccatat cttaggtttc ttatcaggac
     7201 tatataaata ttgtccgggc aaaaattctt ctatcttgca acattgacct tctaacgctt
     7261 ttaactccga agctacataa tacactccat ctttatccca tccttgatat agtgggataa
     7321 tgccaatatg atcacgccca atcatataga cgtttttttc aacatcgtac aatgcaaaag
     7381 caaaaatacc acttagatct tccatgaaat taggcccctt tttactatat aaggctaata
     7441 taacttcaca gtccgatttt gtgagaaatt cgtattgtcc agcaaattgt tttcgaatat
     7501 cctgatgatt gtatatctct ccattaacag caagtactag tttgccgtct ttactatata
     7561 aaggctgttt gcctgatatg gggtcaacaa ttgataaacg ctcatgagcc aatattgcgt
     7621 tttcatttga gtatattcct gaccaatcag gtcctctgtg tcttatcttt ttcgacattt
     7681 ttaatacctg tgtccttaat tcgggtacag gctgttttat ttcaaatgca catacaattc
     7741 cgcacatagt agtaattaat taatggttat aagattcaag tacctgaata gtacttattt
     7801 tatcaagaat ttatctattt cggctgcagc ttttcgtcct gctgcaatag cacgtactac
     7861 caaacttgat cctgtattta cgtctcctgc tgcaaacact tttggtatag aggtttgttg
     7921 gttcgcattt gtggcgacat taccgcgttc atcatattta agccccattt cattaagtaa
     7981 gccttcatgt tttggttgtg taaatcccat tgaaagcaat accaattcaa cttctaatgt
     8041 ttctatttta cctgttttat tcattatagc acgtcctcca tcagggctag gtaaccattc
     8101 gacttcttct acttctactg cagttaattt accatttttc ccaataaact tatttgtagt
     8161 aaggcaccat cgacgttcgc atccttcttg ttgtgaactt gatgtcctta atatattagg
     8221 ccagttaggc catggagtcg cagaattttc tctttcggga ggcatcggca taatttcgat
     8281 ttgtgtaaca cttttagctc cttgacgtat tgaagtgcct acacaatccg aacccgtatc
     8341 accaccacct ataaccaata cacgcttgcc atgtgcagtt aagaaattat tagaatcaat
     8401 gtgtttgccc gaaattattc tattttgttg ctgtaataat tctaatgcaa aatgcactcc
     8461 ctctaaatct cttccttcta taggcaaatc tcgagcttgt tcggcaccca aacataaaca
     8521 tatagcatca aaattggaaa atattttctc agtaggataa tcaataccga ctttaactcc
     8581 tgtcttaaat gttatacctt ctgccttaag tatatttaaa cgtctgtcaa ttactttttt
     8641 gtttagctta aaatcaggaa taccaaatcg aagcaatccg ccgacagctt cattcttttc
     8701 aaataacaca acagtatgtc ctgatttatt aagttgatca gcagctgcca ttccagaagg
     8761 tcctccacca ataactgcaa tattctttcc tgtacgtttc ttaggtggca taggtataat
     8821 ataaccttca ttaaaagcat gttcaattac tgccgcttca ttctcacggc atgtaacagc
     8881 tagatttata ctatgattta aaacacaggc attctcgcat aaagcggggc aaatacgtcc
     8941 tgtaaattca gggaagttat tagtgctttg cagtaattct gcagcctctc ttatcaaacc
     9001 tttatataat aaattttgcc attcagggtt tatattgcct agtgggcatg cccaatgaca
     9061 aaatggtacg ccacaatcca tacagcgtga cgcttgtaac tttctgtctt cagaatttaa
     9121 agtctgctca acttcaccga agtcatactt acgttcgtta ataggacgat atcctgcctc
     9181 tttacgatct atacttaaaa atgcttttgg atttcccatt atagatgatt tttttgtcta
     9241 ttaataatca tattccactt gagctatttt cttttttata gcttcaattt tttcatcatg
     9301 aagtactttt ttatattcga taggagttat ctttataaag tgtcgtaaat actcatccca
     9361 attgtcaagg atacgtttgg ctaatgggct gtttgttaat ttataatgat tcttaatcaa
     9421 agattgtaac tctattttgt ccgaattgtc ctcaattaat gataattcta ccatttccat
     9481 attacagaag taatcgaaat taccttctat gtcaagaacg tatgcaatac ctccactcat
     9541 acctgctgcg aaatttcgtc ctgtagggcc aagtaccaca gtacgtccgc ctgtcatata
     9601 ctcacagcaa tggtcaccga caccttcaac tacagcaata gctcctgaat ttctaacaca
     9661 gaaacgttcg ccaacacatc cgtttatgta tacttcacct gctgtagcac cgtataaaga
     9721 tgtattacct gcaattatat ttttctcggg ctcaaaattg ctattttttg ccgggactaa
     9781 cgagataata ccacctgaca aaccttttcc taaataatca ttagattctc cttctaatct
     9841 aaaacttatt ccgtgtgtta agaatgagcc aaaactttgt cctgccgaac cttcaaaagt
     9901 atagcggata gtatttttag ctaaacctgc atctccataa cgcctcgcaa tttcgcccga
     9961 aagcattgca ccaactgttc tatctgtatt acgaacctta aatgttttat ctacacttaa
    10021 atgtgattca attgcagatt ttgcttcggc aattaatctt atatcaagaa cgttttcaat
    10081 tttatgattt tgttctttaa ccatgtgaaa atcattcttt tcggcttctt ttggtaatct
    10141 aataagtttt ttcagattta tctttttagc tttttcatta acttgagtag gtacaaattc
    10201 taatagttcg ctatgtccta caatatcttc aagtttagta taacccatgg cagctaaatg
    10261 ctctctaact tcttctgcta ggaaattgaa atagtttaca agatattcat atctgccgat
    10321 aaatcgttta cgaagttcct cgctttgtgt tgcaattccc atcggacaag tatttaagtg
    10381 gcatttacgc atcataacac aacctaatac aataagtgca gaacttgcaa atccaaactc
    10441 ttcagcaccc aatagggccg ctgtaataat atccttaccg gtctttaatt gtccatcagc
    10501 ctgtatgcgc acctgtccgc gtaagttgtt cattaccaaa gttttttgag cctctgcaag
    10561 acctagttct attggtaaac cggcatattt aatagaacta gccggactag ctcctgtacc
    10621 accctcacca cctgatatta gtatcatatc cgctttggcc ttagcaacac ctgctgcgat
    10681 agttccaaca cctgcctcag atactaattt tacgctaatg cgagcactag gatttatgtt
    10741 ttttagatca aaaattaatt gtgccaaatc ctcaatagaa tatatatcgt gatgaggtgg
    10801 aggtgaaata agtgaaattc ccggaataga atgtcttgtt tgtgcaataa ttttatcaac
    10861 tttaaagcct ggtaattgtc caccttctcc aggttttgct ccttgtgcaa tcttaatttg
    10921 taattcatct gcattaacca gatattctgc tgtaactcca aaacgtcctg atgcgatctg
    10981 cttaatcgca cttctacgat tcaatccgtc tttacttatc ttgaaacgag ccgggtcttc
    11041 accaccttca cctgtattgc ttttacctcc aatagtattc atagcaattg caatagcttc
    11101 gtgagcttcc ttacttatag aaccgaatga catggcacct gtcacaaaac gttttgttat
    11161 ttctgatgcc ggttcaacat tagagatatc aataggctta cggtctgatt tatattgaat
    11221 catgttacgt aagaaaatag gttcttgttt aaaatctata gaatctgtat attctttaaa
    11281 ctttttgaaa ctaccaaggc gtgttgcaat ttgtaacatt gaaattgttt cagggttcca
    11341 agcatgaaac tcacctgtct tacgataggc atataagccg agattatcca tcaatttact
    11401 ttcattgaaa ttatctccaa atccaagttg atgagcattt atagcttcat cttgtatatc
    11461 atgaatgtcg attcctccta tttcagagat agtacctcta aaatattttt caataacaga
    11521 ctgacttata cctaaagctt caaagatttg tgctccacgg taactcctta ttgttgagat
    11581 tcccatttta gacataactt tgagtaagcc tttgtttaca gctttgatat aatttttttc
    11641 tgcagtatgt aaatctaatt gtaactcatt atcttttact aacttgtcta ttgatgcaaa
    11701 taccatgtaa ggatttaccg cagtagctcc atatccaaat aataaggcga agtgcattat
    11761 ttcgcgaggc tctgcagatt ctaccactat agcaatctgc atacgcttac gtttttcaat
    11821 taagtaatga tgcacagcag aaacagccaa taaagaagga atagctacat gttttttgtc
    11881 aacgcctcta tcacttaata ttatataatt gtattcatca attacggctt gttctgattt
    11941 ttgacataat tcgtctaaag ctgattccaa tgcattaaca cctttctccg gttcaaacaa
    12001 cattggtaat gtcagcgtct taaatccttt gtatattaaa ttctgtaata tgtcaaactg
    12061 tctattgctc aatatagggc tctctaattt taccatttta cagagttttg gtgaaggcac
    12121 taataggttg ccttcctgag caccaatata caatcttaat gacattacta attcttctcg
    12181 aataggatcg ataggagggt tggttacttg cgcgaataat tgacgaaagt acgagaacaa
    12241 gcgttgaggt ttgtttgata atactgcaag tggtgtatca ttacccattg atgatgttgg
    12301 ctccttacca tcctgtacca ttggaataat aagtttttca aaatcttctt tgtaatatcc
    12361 gaaagcgcga agtaagcgat aataattttc aacttcatat ttagctgttc tacccgttga
    12421 tattttatca agttcaattc tattcttaga taaccattca ctatatggat atgcttttgc
    12481 taattcatct tttagtagag catctgaata aatttcgccc ttttcagtat caattagtaa
    12541 aatcttacca ggtttaagac gccctttttc ttttatgtca tcaggatcaa tttctaaaac
    12601 acctgcttct gatgcaataa ttatagtgtc tttttttgtg atagtatacc ttgcaggacg
    12661 caatccgtta cgatcaagca tgcctccggc gtatcttccg tcactaaaca atatagctgc
    12721 cggtccatcc catggttcca ttataataga atgatattca tagaatgatt ttaaacaatt
    12781 actgattgga tttttgtcat tccaactttc aggaacaagc atagacaagg catggggtat
    12841 gcttttaccc gtcattaaaa ggaactccaa aacattatct aatgatgcgc tatcgctcat
    12901 atatggttga actataggat acatatcatc catgttacct aacttatcgc attgcataat
    12961 actttcgcgt gcctccatcc agaatcgatt accttttata gtgttaattt ctccattatg
    13021 gccaagtaag cgaaatggtt gagctaaatc ccatgtgggg aatgtgttag tactaaatct
    13081 agaatgtact aaagccaaac ctgatgtata atatggactt gttaagtcag ggaagtattc
    13141 acgaagttgt aatgaagtta acatcccttt gtaaactata cgtttcgatg ataaactaac
    13201 gatgtaaaaa ttgcgttttt gacttaaatt agactttagt gcgactttct ctattttttt
    13261 gcgaataaca taaagtttta gttcaagctt atcctgatct gcttcccctg tgataaatat
    13321 ttgtttaata ttaggctcat tcgagcgcga tatttcgccc aaaatatcac tatttgtagg
    13381 gacatcgcgt acagccaaca tttttaatcc ttcattttct acaatagtat ttataatgtt
    13441 aatacaaaca gccgtctcgt ccttttcttt aggaaaaaat actaatccgg taccatatgt
    13501 tcctttttca ggtacaggta taccttgtag taaaatgaat tcatgtggga tttgtgttag
    13561 aataccagca ccatctccgg tcttattgtc agcactttca gctccgcgat gcaacatatt
    13621 ttccaatatc tgaagacctt tttctactat gtcatgcgtt ttagcaccat gtacgtgagc
    13681 gatcaaccct acaccgcagg cgtcatgctc aaattcgggt ctgtatagat catgtccctt
    13741 cgtatccatc ttatttattt tttcaaaaaa aatatgcaaa agtttattaa tgcatggctt
    13801 ttgcctaaat tttgttattt atttaatgca aaattagaca ttttgatatt ttgctagttc
    13861 aaaatataca tctatttgat atgtaaaata ccaaatataa cattatgtag taaattgtgc
    13921 ataataaact acaaattgta aacaaataat gtgtatttgc ttacaatttg tatattgata
    13981 tgaaaataaa ccagctagat tgtaatattt tatattactt tattgtagat gcttattgat
    14041 ttcctgctca gtcaatacat atgaaatacg ttttccatca ggagtataga cttggtctga
    14101 gcattgtttt aaagaattca taatatatga tgcatcttct gtagaaatag tatttccctt
    14161 tcttgttgat atgtgttttg ccatatctaa tgcaatagtt ttatgtaaat cttcagccaa
    14221 ggcgtttggg ctttcgttag ctgctgttat tgtatcgtgt attaattgaa ttggattgca
    14281 atcttccatt tgagaaggaa caccgattat ctggtatgcg tttttcccaa agtcttcaat
    14341 tataaatcct atagagcata agtcttcacg tatttcagaa aacatactat tttctatcgt
    14401 tgacaactct aaaatttcag ggaataataa tttctgtgat gcgctatgat ttttagtcat
    14461 agcactaaca aaattttcgt ataaaatacg tatatgcgca cgttgatgat tggtaatctt
    14521 tattccatta acatctgatt gaattaaata tttaccaaac aattgaaaac ctgaaaactt
    14581 agtatttgcg ttttctttgt aatcaagact atattgccta tcatcatcat cgcttgtttc
    14641 tattttagtt tcaatcttgt ggtcaagatg gttaatttca tacaatttgt cccaactatc
    14701 aatatttttt tttgaagagt acgaactttc gctatttgta tttgcaaaag gattatattg
    14761 tgggttgtat gatatttttg gaggagcaac atgttcaata tctgaattta atattggtat
    14821 agatggtgca tcgtcttggt caaaattgat agatggaatc acgttcgctt taccaagggc
    14881 ttctttaata actgcagtta tgattggaaa aattgatgat tcgtcttcaa atttgacctc
    14941 tgttttagta gggtgtatat taacatcgat ttttgatgga tcaacatcaa aataaataaa
    15001 atatgatgct ttctggtcgt ggggtaagat tttttcgtaa gcaatactta cagcc
//
LOCUS       DAOPID010000008        14207 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_4966_length_14207_cov_4.878321, whole genome shotgun sequence.
ACCESSION   DAOPID010000008 DAOPID010000000
VERSION     DAOPID010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 14207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14207
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4966_length_14207_cov_4.878321"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="PK641_00745"
     CDS             <1..878
                     /locus_tag="PK641_00745"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HPG54917.1"
                     /translation="FTVSNNLEMKYWSAKDTTGKAKKITLIDNFSFGTSYNIMADSLN
                     WSNINLNLRFKIYNQFTMNIDFVLDPYTYQLNAFGSPTKVNVSQVEKNGVLGRLQSTG
                     TSFGYTFSNETFKKKKKITTPLPSNNEGELPGESSELAQNIASLDPMTEMTEKEKRKE
                     RYKKEDADYQEFKVPWSLSLNYSIRYAYSTFNYDIMEYDRKLTHNLSISANINFTKTW
                     RFSVSSYYDITNNEWTYVNCTLSKDLHCWQMSASFVPIGRYTTYNFMIGVKSTLLQDL
                     KYEKRKDTSDNINWF"
     gene            900..1448
                     /locus_tag="PK641_00750"
     CDS             900..1448
                     /locus_tag="PK641_00750"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPG54918.1"
                     /translation="MDKKNYYLALTYDLFVGEEQELMEQATIEKPLCFYSGLGMMLDK
                     FEEATVGLEMNNTFDFVISKADAYGEYDNESVIDLPKNIFEVDGKIDNKVLFEGNVVP
                     LQDQEGNRINASIVAVGEDTVTVDLNHPLAGEDLHFVGKVLVKREADEDEIRNLMSQH
                     SCGSCHGDCEGEDCESGCCGCK"
     gene            1478..2491
                     /gene="aroC"
                     /locus_tag="PK641_00755"
     CDS             1478..2491
                     /gene="aroC"
                     /locus_tag="PK641_00755"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPG54919.1"
                     /translation="MNTFGNIFRLTEFGESHGRAIGGVIDGCPAGILIDETFVQSELD
                     RRKPGQSALTSPRKESDIVEFLSGVFEGKTTGTPIGFIIRNSDQHSSDYENVKNVFRP
                     SHADYTYTAKYGLRDYRGGGRSSARETACRVVAGAVAKLALNQKFEDSISIDAKIIQI
                     GKVIGTEAEMNAEILKAKQDCDTVGGIISCTIKNVPFGLGEPVFGKLQALLASAMMSI
                     NAAKGFEYGTGFEGASMRGSEQNDEFICQNGQVKTKTNRSGGIQGGISNGQDIYFRVA
                     FKPVATLMKAQNTIDADCNSVVLTMKGRHDACVLPRAIPVVESMAAIVLFDAYLMSRS
                     SRI"
     gene            complement(2488..3807)
                     /gene="tilS"
                     /locus_tag="PK641_00760"
     CDS             complement(2488..3807)
                     /gene="tilS"
                     /locus_tag="PK641_00760"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HPG54920.1"
                     /translation="MQQKIAEYIKKFRLIKPNSKLIIALSGGADSVVLNHILHKMGYN
                     CIAAHCNFQLRDAESDRDELFVRQLCNVLNIPLEIIHFNTNEYAAKQKVSIEMAARKL
                     RYDWFENIRIKYDADFICVAHHKDDSIETCLLNIIRGTGINGITGIKPINGKIIRPLL
                     CVSRKEIEDYAKQNDLSYVTDSTNLETEYTRNKIRNILLPMLEDINPVFRQTMENNIE
                     NWKEAAFLYSHTINNDLSNLCKTDGTYTWICEDELFAFPYSKTLLFEWLKQYGFSNSV
                     IEEIAEHKYTQTGKRFCSDTHELIVDRCRLILSEKKSDDYKTYEISKNDSSCIQPIHL
                     KMSFVFDTSICKDTKVALLDADKLKFPLTIRKWRNGDFFYPLGMESPKKLSDFFVDTK
                     INIAQKQDIYLLVSGNDIVWIIGKRIDNRYKINANTKKVYRLELCDN"
     gene            3930..4499
                     /locus_tag="PK641_00765"
     CDS             3930..4499
                     /locus_tag="PK641_00765"
                     /inference="COORDINATES: protein motif:HMM:NF019959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HPG54921.1"
                     /translation="MKKTRQLFIETARQVFVKMGVAHATMNDIAEASNKGRRTLYTYF
                     KNKEDVYYAVVEQELAHLVERLEKIIDKKIPANEKLVEYIYIHLDSIRDIVQSNGTLR
                     ADFFNDIKTVERIRRQTSIKELKMLKVILREGVNSGLFGIKDIDTAATIILYSLKGLE
                     VPYIKENVSERMAERKEAIAAFIFKGLRP"
     gene            complement(4511..5680)
                     /locus_tag="PK641_00770"
     CDS             complement(4511..5680)
                     /locus_tag="PK641_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG54922.1"
                     /translation="MKHFNHYIIGCFFLLFVVSCSSTDPKAYKVDSEFEPYVQKFFQD
                     AKTYGQNYDSLGLIMRFANLSDNTAGLCYVNRQPITIEIDRTYWNSISGPNETNIKED
                     LIFHEMGHGFPKRTHINNSLNNGDWKSIMCGDELPNGREPNINYRGFRKDYYIKELFT
                     QTNEEPSWSNYVPNFSEIQENDIIYADAQTPSYWFITIPNEEYSGRIESNQYVFENKT
                     ETDLFLPIKFNGLDIRNDFYYEVSARLQSENNNSYFGISIGDTTESSWSIHYSSFNKT
                     KNILIGESSCRQSFVSLYNEGLSETEDNIIALRKKADTLYYYQNGNFYYHNDLIDIPI
                     HGNCFGFTMPAKSTVYINYAKIKSSSESGKKKAFESTDKIRPIPIPKYRTIKYKQ"
     gene            complement(5677..6981)
                     /gene="murA"
                     /locus_tag="PK641_00775"
     CDS             complement(5677..6981)
                     /gene="murA"
                     /locus_tag="PK641_00775"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPG54923.1"
                     /translation="MASFVIEGGHRLSGEIYPQGAKNEALQVICACLLTESTVTINNV
                     PDIADVNNLIELIHQIGVEITHKKEKTYTFCAKNIRHEYFETEDYYKKSSSLRGSIML
                     VGPLLARLGYAVAPKPGGDKIGRRRLDTHFEGMRKLGANFTYDEVKQLYRIESKKLQA
                     CYMLLDEASVTGTANILMSAVLADGVTTIYNAACEPYIQQLCKMLNSMGAKIEGIGSN
                     LLTITGVNSLKGCEHTILPDMIEVGSFIGMAAMTGSDITIKNVSIQNLGLIPDSFRKL
                     GIQLECTRDDIHIPAQEHFSIDSYIDGSIMTIADAPWPGLTPDLLSVLLVVATKAKGS
                     VLIHQKMFESRLFFVDKLIDMGAKIILCDPHRATIIGLGDKFRLRAANMSSPDIRAGI
                     ALLIAALGAEGTSTINNIEQIDRGYEHIDERLNALGANITRK"
     gene            complement(6983..7594)
                     /locus_tag="PK641_00780"
     CDS             complement(6983..7594)
                     /locus_tag="PK641_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HPG54924.1"
                     /translation="MEYYTQKNKLILTEYGRNIQQMVEYAMTIEDRDERTRCVHTIIN
                     IMGNLFPHLRDVNDFKHKLWDHLAMMSNYKLDIDYPYEVPKELVLTKPEPLPYPETSV
                     KYRYYGQQMIQFIKAVEDYNGDNKDKLVTLLANHMKKCFLTWNKDNVDDQKIFNDLRE
                     ISNGKIDLNEETIKLKETKDILANNNKKIFVNGKNRQKQQGRK"
     gene            complement(7604..8293)
                     /gene="tsaB"
                     /locus_tag="PK641_00785"
     CDS             complement(7604..8293)
                     /gene="tsaB"
                     /locus_tag="PK641_00785"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPG54925.1"
                     /translation="MASILNIDTATRICSAALSEDGFCVAQKLDIEGKNHGKLIAGFV
                     GELMNEAKQRNRKIDAIAISCGPGSYTGLRIGSSFAKGLCYGLDIPLIAIPTLKLIAK
                     GFIEKQGFVDGLLCPMIDARRMEVYAALYNPDLSEHRETSADIIDENSYAEYLNSGKV
                     YFFGDGSEKCQTSILHKNAIFVNDIIPLSENMASLSEAAYKSSDFKDIAYFEPFYLKE
                     FISTKPKNKVF"
     gene            8397..9272
                     /locus_tag="PK641_00790"
     CDS             8397..9272
                     /locus_tag="PK641_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HPG54926.1"
                     /translation="MIQSMTGFGKSVCEFQNKKIVAEIKSLNSKQMDISTRVSGLYRE
                     KDIEMRSRIAQVLERGKVDFALYTDNTSAEANCHINTSVFEDYRKQIVALAAATGLEE
                     PKDWFQLLLKIPDVMKTEVQDLDEDEWNVAQTAIDNAISQLITFRKQEGEGLEKFFLS
                     KITNIENLLAEVPNYERARIDKIKSRFEDNLKSLEDKITYDQNRVEQELIFYIEKLDI
                     SEEKQRLANHLKYFRDTLANGESQGKKLGFIAQEMGREINTLGSKANHSELQIVVVKM
                     KDELEQIKEQVLNVL"
     gene            9276..9842
                     /gene="gmk"
                     /locus_tag="PK641_00795"
     CDS             9276..9842
                     /gene="gmk"
                     /locus_tag="PK641_00795"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HPG54927.1"
                     /translation="MDSKLIIFSAPSGSGKSTIIKYLLDQNLGMEFSISATSRQPRGT
                     EKNGIEYYFLSPNEFKAKIAAGEFLEYQEVYKNRFYGTLKSEVERIAAKGNATIFDVD
                     VLGGLNIKKMYGEKALTIFVQPPSILELRHRLELRGTDSPEVIAQRIEKAEFEMTFAN
                     QFDKIIVNDNLQVAQRNAAEFIREFLLR"
     gene            9805..10437
                     /gene="nadD"
                     /locus_tag="PK641_00800"
     CDS             9805..10437
                     /gene="nadD"
                     /locus_tag="PK641_00800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HPG54928.1"
                     /translation="MPPNLYENFFLDSDVAELKRVGLYFGSFNPIHNGHLSLARFILA
                     NTNLQKLWFVVSPQNPFKPQSILADDMMRLEMVRIAIEDEADMDVLDVEFSLSKPSYT
                     IDTLQYLSKKNPHIEFSLLIGADNVEDFCKWKDYQKILNEYDVLVYPRNGYDLSETNN
                     KYPEMHCLEAPIFDFSSSDVRNLLREGKDVSDLIKPEVLKYIKSHHIYNS"
     gene            complement(10469..11350)
                     /locus_tag="PK641_00805"
     CDS             complement(10469..11350)
                     /locus_tag="PK641_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPG54929.1"
                     /translation="MRKSIVLCLIGLCAASIGMAKSSQSRIVIDSIQSKTLNTYRTYS
                     ILLPAGYDEQTDRKYPILYLLHGMWDKNQCWAQKGNLLEVYNRLIASDEIDEMIIVTP
                     NAGGNLFAGEINGYFNMPNWQYEKFFFTEFLPYIESTYRIKTDKKHRAIAGLSMGGGG
                     SVSYAQRHPELFQSVYAMSASLSLEERKKNIQENSELEILRKDLDDSIEKNDCVNFVT
                     SADSSKIKQLKSIKWFIDCGDDDFLFDCNVRFVQAMKKASIPCQFRVRDGGHDWEYWH
                     SALYICLPFVNRGFEKP"
     gene            complement(11401..11874)
                     /gene="rlmH"
                     /locus_tag="PK641_00810"
     CDS             complement(11401..11874)
                     /gene="rlmH"
                     /locus_tag="PK641_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HPG54930.1"
                     /translation="MKIILLVVGKTNDEYINKLIDNYIQRLKHYTDFEWQTIAELKNT
                     KNMPEGTQKEKEGEAILKATENADEIFLLDEHGDQPTSVEFSGLISKYMNKGTRKLVF
                     VIGGPYGFSEAVYKKITKKISLSKMTFSHQMVRLIFVEQLYRAFTIIKGESYHHE"
     gene            11964..12359
                     /locus_tag="PK641_00815"
     CDS             11964..12359
                     /locus_tag="PK641_00815"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HPG54931.1"
                     /translation="MKNTSLFAIFWMMLVSLPIYSQVDDSQRLISCISKGDMSALSKY
                     FDPEVEISILGKGDILSAKQAESALSDFFSGKLVSSCKISHQGNNGKVSFCIITLTMS
                     NREQYRVYVLNKKVGEQPKIQQFRIDYVD"
     gene            12349..13194
                     /gene="nadC"
                     /locus_tag="PK641_00820"
     CDS             12349..13194
                     /gene="nadC"
                     /locus_tag="PK641_00820"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HPG54932.1"
                     /translation="MLIDNNLTEEIKYWFNEDIGDGDHTSLSCIPQTEMGKSRLIIKD
                     TGILAGVEVAEKIFHSFDPDLKMTVFLKDGASVKPGDVAFEVEGKVLSLLQTERLMLN
                     IMQRMSGVATITAKYVKRLEGLKTKVLDTRKTTPGLRLLEKQAVKIGGGENHRIGLYD
                     MILLKDNHVDFAGGIENAITSAKKYCAEKGKNLKIEIEVRNFDELNRVLNLGGVDRIM
                     LDNFTPENTRKAVELIAGKYETESSGGITYDTLRTYAECGVDYISVGALTHSVKSLDM
                     SFKAV"
     gene            13195..>14207
                     /locus_tag="PK641_00825"
     CDS             13195..>14207
                     /locus_tag="PK641_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPG54933.1"
                     /translation="MSNNDSLSLGTERIWILLRRYAVPSIVAMTASSLYNMVDSIFIG
                     QGVGTMAIAGLAITFPFMNLAAAFGAMVGVGASTVISVKLGQKDIEGAEHAMGNVVLL
                     NIMIGIVFMMVSLLFLDNILIFFGASEATLPYARDYMEIILYGNIVTHVYLGLNAVMR
                     SSGYPRGSMYTTLFAVIINGFFNYLFIFVFNMGIKGSALATVIAQILALIAVLWHFSR
                     PSTFLRFKLSMFKFRLTVARNILAIGLAPFFINVCACLVVMLINNGLKTYGGDLYIGA
                     YGINNRIVFFFIMIVMGLNQGMQPIVGYNYGARKLDRVMSGFKMTAISAVAVTTIGFL
                     ICQ"
BASE COUNT         4483 a         2259 c         2636 g         4829 t
ORIGIN      
        1 cattcacagt atcgaataat ttggaaatga aatattggag tgcaaaagac actacaggga
       61 aagctaagaa aataactctt attgataact ttagtttcgg aacttcatat aacataatgg
      121 ccgattcgct taattggtca aatataaact taaatcttcg tttcaaaata tataatcaat
      181 ttacaatgaa tattgatttt gttttggatc cttacaccta tcagcttaac gcgtttggtt
      241 ctccaactaa agtaaatgtt tcgcaagttg aaaagaatgg tgttttagga cgtttacaaa
      301 gtacaggtac atcttttgga tacaccttct caaacgaaac tttcaagaaa aagaagaaaa
      361 taacgactcc acttccctct aataatgagg gtgaattacc cggtgagtct tccgaattag
      421 cgcaaaatat tgcttcctta gatcctatga ctgaaatgac tgaaaaggaa aaaagaaagg
      481 aaaggtataa aaaagaagat gcagattatc aggaatttaa ggtgccatgg agtttaagtc
      541 ttaattatag tatccgatat gcatactcaa catttaatta tgatataatg gagtatgatc
      601 gcaaactaac gcataacctg agtatttcgg caaatataaa tttcacaaag acatggaggt
      661 ttagtgtatc atcctattat gatattacca ataatgaatg gacttatgta aattgtacac
      721 tttcaaaaga cttgcactgt tggcaaatgt ctgcaagttt tgttcctatt ggcagatata
      781 caacttacaa ctttatgata ggcgttaagt ctacattatt gcaagatttg aagtacgaga
      841 aacgaaagga tacatcagac aatataaact ggttttagta atcatttaat tataaaaata
      901 tggacaaaaa gaattattat ttagcactta catacgattt atttgtaggt gaagaacaag
      961 agttaatgga gcaagctaca atagaaaagc ctctttgctt ttatagtggt ttgggcatga
     1021 tgttggataa gtttgaagaa gcaacagtag gactcgaaat gaacaataca tttgattttg
     1081 taatatctaa ggctgatgct tatggagaat acgataatga aagtgtaatc gatctgccta
     1141 aaaatatttt tgaagttgac ggcaaaattg ataataaggt cttgtttgaa ggaaatgttg
     1201 tacctttgca agatcaagaa ggaaatagaa ttaacgcttc gattgttgct gttggagaag
     1261 atactgtaac tgtagattta aatcacccat tggccggtga agatttgcat ttcgttggaa
     1321 aagtattagt taaaagagaa gctgatgaag atgaaattag aaaccttatg agtcaacatt
     1381 cttgtggttc ttgccatggc gactgtgaag gtgaagactg cgagagtggt tgttgcggat
     1441 gtaagtgata tagtgactaa taaataattt tggcgatatg aatacatttg gaaatatatt
     1501 cagactgact gaatttggtg aatctcacgg acgtgccata ggcggagtta ttgacggatg
     1561 ccctgcaggt atcttgatag acgagacatt tgtacaaagt gaattagaca gacgcaagcc
     1621 tggtcaatca gctttgacaa gtccacgtaa agaatcggat atcgtagaat ttttatccgg
     1681 tgttttcgaa ggtaaaacta caggaactcc gataggtttt attatccgta attctgacca
     1741 acattcgtcg gattatgaaa atgtaaaaaa tgtattcaga ccttcgcatg ccgattatac
     1801 ttacacagca aaatatggac tgcgtgatta tcgcggtggt gggcgtagct cagcacgaga
     1861 aactgcatgt cgtgtcgttg ccggagcagt agctaaactt gctttaaatc aaaagtttga
     1921 agattctatt agtatagacg caaaaattat tcagatagga aaagttatag gaactgaggc
     1981 tgaaatgaat gcagagattc taaaggctaa acaagattgt gatacagttg gcggaataat
     2041 atcttgtacc atcaagaatg tgccattcgg tttaggagaa cctgttttcg gtaaattgca
     2101 ggcattgctt gcatctgcaa tgatgtcaat taatgcggct aagggatttg aatatggaac
     2161 aggttttgaa ggagcttcaa tgcgtggctc tgaacaaaat gacgaattta tttgtcaaaa
     2221 cgggcaagtt aaaactaaaa ccaatcgttc cggtggtatt caaggaggaa tctctaatgg
     2281 ccaggatatt tactttagag ttgcctttaa acccgttgca actcttatga aagcgcaaaa
     2341 tacaattgat gcagattgta attctgttgt tttaactatg aagggaagac atgatgcttg
     2401 tgtattgcct cgtgctatac ctgttgttga atcgatggct gcaattgtat tgttcgatgc
     2461 ttatttaatg tcgcgtagct cgcgtattta gttgtcgcaa agttctaatc gataaacttt
     2521 tttagtattg gcattgattt tatatctgtt gtcgatgcgt tttccaataa tccatacaat
     2581 atcattgcca gaaacaagta agtaaatgtc ttgtttctgt gcaatgttta tttttgtatc
     2641 cacaaaaaaa tcactgagtt ttttaggact ttccattccg agtggataaa agaaatcgcc
     2701 attgcgccat tttcttattg ttagcggaaa ctttaatttg tctgcatcaa gcagagctac
     2761 ttttgtgtct ttacaaatac ttgtatcaaa aacgaaactc atttttagat gtatgggttg
     2821 tatgcaagag ctatcatttt tacttatctc ataagtttta tagtcatctg atttcttttc
     2881 agacaatatc aatctacatc tatcaactat tagttcgtgc gtgtctgaac aaaagcgctt
     2941 gcctgtttga gtgtatttgt gttcagctat ttcttcgata acactatttg aaaatccata
     3001 ttgttttaac cattcaaaca gcaatgtttt tgaatatggg aatgcaaaaa gttcatcttc
     3061 gcaaatccat gtatatgtac catctgtttt acataaattc gataagtcat tatttatagt
     3121 gtgcgaatac aaaaaagcag cttctttcca attctcaata ttgttttcca tcgtctgcct
     3181 aaataccgga tttatatctt ccagcattgg cagtaaaata tttcttatct tatttctggt
     3241 atattcagtt tctagattag ttgaatctgt aacataacta agatcgtttt gctttgcata
     3301 atcttctatt tctttacggc tgacacataa caatggtctt attattttgc cattaatagg
     3361 cttaatgcct gtgataccgt taatcccggt tcctcttatt atatttaaca ggcaggtttc
     3421 tatagaatca tctttatgat gagcaacgca aataaaatcg gcatcatatt taattctgat
     3481 gttttcgaac caatcatagc gaagcttgcg agcggccatt tcaatagaaa ctttttgttt
     3541 ggcagcatat tcatttgtat taaaatgaat aatttcaagt ggtatattta aaacattgca
     3601 taattgcctt acaaataatt catctctgtc tgattcagca tctcgcaatt gaaaattgca
     3661 atgtgcagca atacaattgt atcccatttt gtgaagaata tggttcaata caacggaatc
     3721 agcccctcca ctcaaggcta taattaactt tgagttaggt ttgattaatc taaatttttt
     3781 aatgtattcc gcaatttttt gctgcatata aattaaaata ttacaaagat acttttttct
     3841 attgatttga gtttgcagat ttaatgtata aatagtattt ttgcacaaat gaatgatttt
     3901 gtgcaaaaat actatagtaa aattcagtaa tgaaaaaaac gcgtcaactt tttattgaaa
     3961 cagcaaggca ggtgtttgta aagatgggtg tggcacacgc aaccatgaac gatattgcag
     4021 aagcttcaaa caaggggcgt cgtacactat atacgtattt taaaaataaa gaagatgtct
     4081 attatgctgt agtggagcaa gaattagcgc atcttgtaga gcgtttggaa aaaataatag
     4141 ataaaaaaat acctgcaaat gaaaaattgg tagagtatat atatatacat ttagatagca
     4201 tccgcgatat tgtacaaagt aatggtacac ttagggccga cttctttaat gatataaaaa
     4261 cagtggagcg tataagacgt cagaccagca ttaaagaact aaaaatgtta aaagttattt
     4321 tgcgcgaagg tgttaattcg ggattatttg gaattaagga tatagatact gctgcaacaa
     4381 ttatcctata ctctttaaaa ggcttggagg ttccatatat caaagaaaat gtttcagaac
     4441 gtatggcgga acgaaaagaa gctatagctg catttatctt taaaggctta cgtccttaaa
     4501 taatcaattc ttattgtttg tatttaattg tcctatattt cggtataggg ataggacgta
     4561 ttttatcggt agattcaaat gccttcttct taccactctc gctcgatgac tttatttttg
     4621 catagttgat gtaaaccgta ctttttgcag gcattgtaaa accgaagcag ttgccatgta
     4681 taggtatatc aatgaggtca ttgtgataat aaaaattccc gttttgataa tagtataatg
     4741 tgtcagcctt ttttctgagt gcaataatat tgtcttcagt ttcggataga ccttcattat
     4801 ataaacttac aaatgattga cggcatgaac tttctcctat caatatgttt ttggttttat
     4861 taaaagaaga gtaatgtatc gaccatgaag attcggttgt atcgccaata cttataccaa
     4921 aatagctgtt attattttct gattgcaaac gtgctgaaac ttcgtagtaa aaatcattac
     4981 ggatgtctaa tccattaaat ttaatcggta aaaataaatc agtttctgtt ttattttcaa
     5041 aaacgtattg attactttcg atgcggccgg aatattcttc gttcggaata gttataaacc
     5101 aataacttgg agtttgtgca tctgcatata taatatcgtt ttcttggatt tctgaaaaat
     5161 taggaacata attactccaa gaaggttctt cgtttgtttg cgtaaataat tcttttatgt
     5221 aataatcttt acggaatcct cggtaattta tgttcggctc tcttccgtta ggaagttcat
     5281 ctccacacat aatgcttttc caatctccat tatttaagga gttgttaatg tgcgttcgct
     5341 taggaaatcc gtgtcccatt tcatgaaaaa taagatcttc tttgatgtta gtctcgtttg
     5401 gacccgaaat gctgttccaa tatgtcctat caatttctat tgtaataggt tgacggttaa
     5461 cgtaacataa gcctgctgta ttatcgctta aattggcaaa acgcataatc agtcctaaac
     5521 tatcgtagtt ttgaccatat gtttttgcat cttgaaaaaa tttttgaaca taaggctcaa
     5581 attcggaatc tactttgtat gctttgggat ccgttgaact gcatgataca acgaaaagaa
     5641 ggaaaaagca gcctataata taatggttga aatgtttcat ttgcgagtga tgtttgctcc
     5701 taatgcattt aatctttcat caatatgttc atagcctcta tctatttgct caatattatt
     5761 aatagtgctg gtaccctcag ctcctagtgc agcaattaat aatgcaattc cggcacgtat
     5821 atcaggcgaa ctcatattcg cagctcgtag tctgaactta tcacctaagc caataatagt
     5881 ggctctatgt gggtcgcaaa gaataatttt tgctcccata tcaattagtt tgtcaacgaa
     5941 aaataatcta ctttcaaaca tcttttgatg tatcaatacg ctgcctttgg ctttagtggc
     6001 tacaacaagt aaaacgctta gtaaatcagg ggttaagcct ggccatggag catcagctat
     6061 tgtcataata gaaccatcta tatatgaatc tatactaaaa tgttcttgtg ccggaatgtg
     6121 aatatcatct cttgtacatt ctaattggat gcccaatttg cgaaaactat caggtataag
     6181 acctaagttt tgtatagata catttttaat tgtaatgtca gatccggtca tagctgccat
     6241 tccgataaaa ctgccaactt ctatcatatc aggcagaatc gtatgttcgc aaccttttag
     6301 tgagttcact ccggtaatag taagcagatt agatcctatt ccttctattt tagctcccat
     6361 actgttaagc atcttgcata attgctgtat gtatggttcg catgcggcat tatatattgt
     6421 tgtaacacca tctgccaaaa ctgcactcat aagtatattt gcagtacctg taacagaagc
     6481 ctcatcaagc aacatatagc aagcttgtag ctttttactt tctattctgt ataattgttt
     6541 tacttcgtcg taagtaaagt tggctcccag tttgcgcatc ccttcaaaat gggtatctaa
     6601 acgccggcgt cctattttat ctccacccgg tttaggtgct actgcatatc ctaaacgtgc
     6661 taatagtgga ccgaccaaca ttatagagcc tcttaatgag ctactttttt tgtagtaatc
     6721 ttctgtctca aaatattcgt gtcgaatgtt ttttgcacag aaagtgtatg ttttttcttt
     6781 tttgtgtgtt atttcgacac ctatctgatg aatcaactca ataagattat tgacatccgc
     6841 aatatcaggc acattgttta tcgtaacggt actttcggta agtaagcacg cacaaataac
     6901 ctgaagggct tcgtttttcg cgccctgagg ataaatttct ccacttagtc tgtgtccgcc
     6961 ttcaataaca aatgatgcca tattattttc ttccttgttg tttttgacga ttcttaccgt
     7021 ttacaaaaat cttcttattg ttgttggcaa gtatgtcttt agtttctttt aacttgatcg
     7081 tttcttcgtt taaatcgatc ttaccatttg agatttcgcg caaatcatta aatatttttt
     7141 ggtcatcaac attgtctttg ttccatgtta ggaaacattt tttcatgtga tttgccaaca
     7201 atgttaccaa tttatcttta ttatcgccat tataatcttc tacagcctta ataaattgta
     7261 tcatttgttg tccgtaataa cgatatttta ctgaagtttc aggatatgga agtggttccg
     7321 gttttgttaa aactaattct tttggtacct catatggata gtcaatgtca agtttataat
     7381 ttgacatcat agccaagtgg tcccataatt tatgcttaaa gtcgtttaca tcacgcaaat
     7441 ggggaaataa atttcccatt atgttgatta tggtatgtac acaacgtgtg cgttcgtccc
     7501 tgtcttcaat cgtcatggca tattcaacca tttgttggat gtttcgaccg tattctgtca
     7561 aaattaattt gttcttttga gtataatatt ccatgatgta aaattagaat accttatttt
     7621 tgggtttggt agatataaat tctttaaggt aaaatggttc aaaatatgcg atatctttga
     7681 aatcgcttga tttatatgct gcttccgata gcgatgccat attctctgat aatggaataa
     7741 tgtcattcac aaagatagcg tttttatgca aaatactagt ttggcatttt tctgatccgt
     7801 ctccaaagaa gtaaactttt ccactattta gatattctgc ataagaattt tcatctataa
     7861 tgtcggcaga tgtttctcta tgttcgctta aatcaggatt atataaagca gcatatactt
     7921 ccattcgacg agcatcaatc ataggacata atagtccgtc aacaaatcct tgtttttcaa
     7981 taaatccttt tgcaatcaat ttgagtgtag gtatggcaat tagcggtata tctaaaccat
     8041 aacataaacc ttttgcaaaa gagctgccaa ttcttaggcc tgtatatgaa cccggaccac
     8101 aactgatagc tattgcatca attttacgat ttcgttgttt cgcttcattc atcaattcgc
     8161 caacaaaacc tgctattaac ttgccatggt ttttaccctc gatatcaagc ttttgtgcta
     8221 cgcagaaacc atcttctgag agtgcagcgg agcaaatgcg tgtagccgta tctatattta
     8281 atatacttgc cataacaaga tgcaaaagta gtgatttatg ctgagatttg actaatttaa
     8341 ttcaaaaaac cttacctttg caaggtttat atctataaaa attaaaacga ttatacatga
     8401 ttcaatctat gactggtttt ggaaagtctg tatgtgaatt ccaaaataaa aaaattgttg
     8461 ctgaaatcaa gtctttaaat agtaaacaaa tggatatttc aactagagta tctggtttgt
     8521 atcgcgaaaa agatattgaa atgagaagtc gtatagcaca agttttggaa cgagggaaag
     8581 ttgactttgc attgtataca gataatacca gcgctgaggc gaattgtcat ataaatactt
     8641 ctgtttttga agattatcga aaacaaatag tcgcacttgc tgctgcaaca ggtttggaag
     8701 aacctaaaga ttggtttcaa ttattactaa agattcctga tgtaatgaaa acagaagttc
     8761 aggatttgga tgaggatgaa tggaatgtag ctcaaacagc gattgataat gctatatctc
     8821 aactaattac atttagaaaa caagaaggtg aaggacttga aaaattcttc ttatctaaaa
     8881 ttacaaacat agaaaatctt ttggcagaag tccctaacta tgagcgtgct cgtattgaca
     8941 aaataaaatc tcgtttcgaa gataatttaa aatcgcttga agataagatt acatacgatc
     9001 aaaatagagt agagcaggaa cttatttttt acatagaaaa gcttgacatt agcgaagaaa
     9061 aacaacgctt ggcaaatcat cttaaatatt ttcgcgatac attggccaat ggagaatctc
     9121 aaggtaaaaa attagggttc attgcgcaag aaatgggacg tgaaatcaat actcttggtt
     9181 caaaagcaaa tcatagtgaa ctacaaattg tagtagttaa aatgaaggat gaactggaac
     9241 aaattaaaga acaagtatta aatgttttat agctgatgga ttcaaaactg ataatatttt
     9301 cagcaccttc aggatcgggc aaaagtacga ttattaaata tttgcttgat cagaatcttg
     9361 gtatggaatt ttcaatatca gctacaagtc gtcagccccg cggaactgaa aagaatggga
     9421 tagagtacta ttttttgagt ccgaatgagt ttaaggcaaa aattgcagca ggcgaatttc
     9481 ttgaatatca agaagtttac aaaaatagat tttacggaac tcttaaatcg gaagttgaga
     9541 gaattgctgc aaaaggcaat gctacaattt ttgatgttga tgttttgggt ggactgaata
     9601 taaagaaaat gtatggtgaa aaggccttga ctatttttgt tcaaccccca agtatattag
     9661 aattgcgcca cagactagaa cttagaggaa cagactctcc tgaggtaata gctcagagaa
     9721 tagaaaaggc agagtttgaa atgacttttg ccaatcaatt tgataaaata atagtaaatg
     9781 ataacttgca agttgctcaa cgaaatgccg ccgaatttat acgagaattt cttcttagat
     9841 agtgatgtgg ctgaacttaa aagagttggt ttgtatttcg gttcatttaa tcctattcac
     9901 aatggccatt tatcgttggc acggtttata ctagcgaata ctaacttaca aaagttgtgg
     9961 tttgttgttt ctcctcaaaa tccgtttaag cctcaatcaa tattagctga tgacatgatg
    10021 cgtttggaaa tggtaagaat tgctatagag gatgaggctg atatggacgt tttggatgtt
    10081 gaattttcct tgtcgaagcc atcatacacg attgatacat tacaatattt atcgaaaaaa
    10141 aatccgcata ttgagtttag tttgcttata ggtgctgata acgttgagga tttttgtaaa
    10201 tggaaagatt atcaaaaaat tctaaacgaa tatgatgtgt tagtgtatcc taggaatggt
    10261 tatgatttgt cagaaaccaa taataagtat cctgaaatgc attgtttaga agcacctatt
    10321 ttcgattttt cgtcatctga tgtgcgtaat ttactgcgag aaggcaagga tgtatcagat
    10381 ttaattaaac cggaagtttt gaaatacatc aaatcccatc acatatacaa tagctaaata
    10441 caccttactt ttcctatgtt aatatcgact atggtttttc aaaccctcta tttacaaatg
    10501 gcagacagat gtataatgct gaatgccaat attcccagtc gtgtccgcca tcgcgaactc
    10561 taaattgaca tggtattgag gcttttttca tggcttgaac aaagcgaaca ttgcaatcaa
    10621 aaagaaaatc gtcatcgccg caatcaataa accacttaat tgattttaat tgttttattt
    10681 tagatgaatc tgcagatgta acgaaattta cgcagtcgtt tttttctatt gagtcgtcta
    10741 aatctttacg gagtatctca agttctgaat tttcctgaat atttttttta cgttcttcta
    10801 acgacagtga tgcactcatg gcataaacgc tttgaaatag ttctggatgg cgttgagcat
    10861 aggatacaga cccgcctcct cccattgaca agcctgctat agctctatgt tttttgtctg
    10921 tttttatgcg atatgtcgat tctatataag gcaaaaactc ggtaaaaaag aacttttcgt
    10981 attgccaatt aggcatgttg aaatatccgt ttatttcgcc tgcaaacaaa ttgcctccgg
    11041 cattaggagt tacgattatc atttcgtcta tttcgtctga agcaataagt ctgttgtaaa
    11101 cctctaataa attacctttc tgagcccaac attggttttt gtcccacatt ccatgcaaaa
    11161 gatacaaaat tggatatttt cggtccgttt gttcatcata tcccgccggc agtaagatag
    11221 aataagtacg atatgtattt aaagttttgc tttgtataga atctataaca atacggcttt
    11281 gcgatgattt tgccattcca attgatgccg cacataatcc tattaagcac aaaacgatag
    11341 actttctcat atttttaatg ttttaattat ttagattatt tctatacaca aaacttaaga
    11401 ttattcgtga tgatatgatt cgcctttgat tatggtaaag gctctatata gttgctctac
    11461 aaaaataagt ctaaccattt gatgcgaaaa tgtcattttt gatagagata ttttttttgt
    11521 gatttttttg taaacagcct ccgaaaatcc gtatggaccg ccaataacaa aaactaattt
    11581 acgtgtgcct ttattcatat acttacttat aagtccggaa aattcgaccg atgtaggctg
    11641 atctccatgt tcatcaagta agaatatttc gtcagcattt tcagtggctt ttaagatagc
    11701 ttcgccttct ttttcttttt gagttccctc aggcatattt tttgtgtttt ttagctcggc
    11761 tatagtttgc cattcaaaat cagtgtaatg ttttaacctt tgtatgtaat tatcaatcag
    11821 tttgttgata tattcatcat tggttttgcc aacaacaagt agtattattt tcatattatt
    11881 ttttaccttt gtgttgtact ctgggctata gtttttgtta tcacgtacaa atgtcggaat
    11941 aaaacctgat aaaatcaaac aacatgaaaa atacatcttt gtttgccata ttttggatga
    12001 tgcttgtttc attgcctata tattcgcaag ttgatgatag tcagagatta atatcttgta
    12061 tatcgaaagg tgatatgtcg gctttgtcta agtattttga tccggaagtc gaaatctcaa
    12121 tacttggcaa aggagatatt ttgtcagcga agcaagctga atcggcacta tctgatttct
    12181 tttcaggtaa attggtttca tcatgcaaaa tttcgcatca aggcaataat ggaaaggtta
    12241 gtttttgcat tattacattg acaatgagta atagagaaca atatagagta tatgtactga
    12301 ataaaaaagt aggggaacaa ccgaaaatac aacaatttag aatagattat gttgattgac
    12361 aataatttaa cagaagagat taaatactgg ttcaacgaag atataggtga tggagatcac
    12421 acgtcattat cttgtatacc acaaacagaa atgggtaagt ctcgcttgat tattaaagac
    12481 acaggtattt tagccggcgt tgaagttgcg gaaaaaatat ttcactcatt tgatcctgat
    12541 ttaaaaatga cggtattttt aaaagatggt gcatctgtta aacccggaga tgttgcattt
    12601 gaagttgaag gtaaggtttt gtcgttgcta caaaccgaaa gattaatgtt gaatattatg
    12661 caacgtatga gtggagtggc aactatcaca gctaaatatg tcaaaaggct ggaaggatta
    12721 aaaacaaaag ttctagatac acgtaaaacg actcccggat tgagattgct tgaaaaacaa
    12781 gctgttaaaa ttggaggagg agaaaatcac cgcattggtt tatacgatat gattttatta
    12841 aaggataatc atgttgattt tgcaggtggt atagaaaatg ccattacttc ggctaaaaaa
    12901 tattgcgcag aaaagggcaa gaatcttaag attgaaatag aggttcgtaa ttttgacgaa
    12961 ttgaatagag tcttgaattt aggaggtgta gaccgaataa tgttagataa ctttacccct
    13021 gaaaatacta ggaaagctgt tgaacttata gcaggcaaat acgaaacaga gtcttcagga
    13081 ggtattactt atgatacttt gcgtacttac gctgagtgcg gggttgatta tatttcagta
    13141 ggagctttga cacactctgt taagagtttg gatatgagtt ttaaagccgt ttaaatgtca
    13201 aataacgata gtctctcttt aggaactgag cgaatatgga ttttgcttag gcgatatgct
    13261 gtgccatcaa ttgttgcgat gaccgcatcc tctttataca atatggtgga tagtatattt
    13321 attggccaag gtgttggaac tatggcaatt gccggtttgg caataacttt cccttttatg
    13381 aatttggctg cggcatttgg agcaatggtc ggagttggag catcaacagt aatatctgta
    13441 aaattaggtc agaaagatat tgaaggagca gaacatgcaa tgggcaatgt tgtcttgctt
    13501 aatataatga taggaattgt atttatgatg gtttctctct tattcctaga taatatactt
    13561 atattctttg gagcaagcga agcaacattg ccatatgctc gtgattatat ggaaataatt
    13621 ttatatggca atattgtaac tcacgtctat ttaggcttga atgctgtaat gcgttcttcc
    13681 ggatatccgc gtggctctat gtatacaact ttgtttgcag ttattattaa tgggttcttt
    13741 aattatctgt ttatttttgt tttcaatatg ggtatcaaag gatctgcctt ggctactgtt
    13801 atagctcaga ttttagcttt aatagctgtg ttgtggcatt ttagcagacc ctctactttt
    13861 ttgcgtttca aattaagcat gtttaaattt agattgactg ttgcgcgtaa tattttggca
    13921 attggtttag ctccattttt tattaatgta tgtgcttgcc tagttgtcat gcttattaat
    13981 aatggtttga aaacctacgg aggtgactta tatataggtg cttatggtat caataacaga
    14041 atagtattct tttttattat gatagtgatg ggcttgaatc aaggtatgca gcctatagta
    14101 ggctataact atggagcaag aaaattggat agggttatgt ccggatttaa gatgacggca
    14161 ataagtgcag ttgcagttac aactataggc tttttaattt gtcagtg
//
LOCUS       DAOPID010000009        13783 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_5184_length_13783_cov_4.599942, whole genome shotgun sequence.
ACCESSION   DAOPID010000009 DAOPID010000000
VERSION     DAOPID010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 13783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13783
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5184_length_13783_cov_4.599942"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PK641_00830"
     CDS             <1..209
                     /locus_tag="PK641_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HPG54934.1"
                     /translation="NGLEFTEHKYIAKMLDTKIFFAHPYCSWEKVTIENTNKLIRQYI
                     PKKLNFTDPKSAFFKNIRNFALAT"
     gene            267..1745
                     /locus_tag="PK641_00835"
     CDS             267..1745
                     /locus_tag="PK641_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG54935.1"
                     /translation="MKRIVVPLFIMTIFLGYSQIPDKRVIISNVSHDNIFADIELKTL
                     DPESAYANLPDGEIDCIKGNTWIFNIEKPKYVRFSANSDGNWIDQFAVIFPGDSVSYK
                     IELVGETDFSHSTKNFKKEYRMLFSGKDAANYNYSYDIRTLYSDTNSYKRPWFQGGMD
                     LMKYREGILAAYESEKKFLSNYNAEHHLSDAFYRYQLDRMKNICIVSLYSPFCSNYNL
                     HIPESYFGNMEIVEDGTDAYYKALQTKYIDSQPPDFDRTYQYIIQNAPKKLHEYLITS
                     LVECYAWRKIGVPSLEKIKSEIQNPVYLARIEENEKKLKEYFRIPLDDYPQEGKPFSD
                     YLLENTYFRSYDNAKKITLKELLNTYQNKAIYIDIWASWCMPCRKDIRESVASKEYFK
                     EKKDQIVKIYTSIDSSVEDWLSASEQEGVIQNQYLCEGGYDALLYQVLKRSGNGIPRY
                     ILLNKEHNIELLNAPRPVQFEGLSGGFEDLKKNIDRMLESSK"
     gene            1774..2097
                     /locus_tag="PK641_00840"
     CDS             1774..2097
                     /locus_tag="PK641_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG54936.1"
                     /translation="MNFVQTLYMSPDNNLFHDSFGWVALEYHLMSWALSCLLLKRSYT
                     NVKLYANAEVAKCLTDYLKLPYDEVHILSDKFKLPHPSLWALPKLWTYSLQEEPFLHV
                     DALLF"
     gene            2288..3259
                     /locus_tag="PK641_00845"
     CDS             2288..3259
                     /locus_tag="PK641_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HPG54937.1"
                     /translation="MKRLLSVVSFFLIFLVVSAQVKTIEYAQRDTCKLYMDVYNPKGQ
                     SDGMCVIYAFGGAFKMGDKTDKVTVSYMEKLSQNGITAIAINYRLGMKGDAYELKGMK
                     LLNRYADAIRMATEDMLTASRYVNDHALELGVDPNKIVLSGSSAGAMTALQCDFELSN
                     NTQLGQIMPDGFRLAGVVTFAGAVSVLNGKIEYKKAPAPTLFFHGTEDALVPYNKISM
                     FGKGMYGSAALAKIFKKHEYPFGIIRYEHFGHEIASIPIHENISEILRFLDTYVRDRK
                     AANFDLTIHETAVTKPMLNFSIRSINKMQEIKISKEAQSRMSGGESR"
     gene            3274..4059
                     /locus_tag="PK641_00850"
     CDS             3274..4059
                     /locus_tag="PK641_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPG54938.1"
                     /translation="MKKTPKYKIGLALSGGGVKGFAHAGAFKALEEYGIKPQIISGTS
                     AGSVAGVLYSSGYKPQEIYKMFFSKGVNNFVALTLPVSGFFNPSKFVQFLRKQIKYEN
                     LEDLPIPVRVVATDLDHGKSVVFTKGRLAERVMASSTIPIVFVPTEIDGVHYVDGGIF
                     CNFPVSAIREDCEKVIGVNVSPLVPTEYKQTLVGIAERSYNFMFRANTIEECKKCDII
                     IEVADALKYSNLDLSKVEDIYRLGYESMVQELEKNKDILDDFR"
     gene            4072..5316
                     /locus_tag="PK641_00855"
     CDS             4072..5316
                     /locus_tag="PK641_00855"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPG54939.1"
                     /translation="MIRRIFITLFFVCIFSSAYATIINGIVKDKQSVPVSFASVYLNN
                     NPTVGTITDSEGHFSLEIENFKSDVLIISFIGYETIRYPLSDLKGNTNLSFTLKEQPI
                     LLQETLVSAKNPSKKMTKRDKRNILKAVRKRMDIDFPNQSCGYKIVSDYIIYNQDDVV
                     AFEELVGTLVEIPSLKRKNGRDSIQLKGDLCKRYRNQQMESKFNNLSANGLNDKYKQV
                     AGRVDSVVMIHSTMWGSEIQWLFDEFIGNTSKWTFTDKGDYTVLNYFDRRNYLGILII
                     ELNLNYVIDSYSYSVQKISQDMTIKANIPFGYKLSKEQLALFNVFNIGENQIEKFRLK
                     TVDTHIKRNIIYKNTDTRVVVSEKNVELKSKMADKNGKGMNVHSTAAIKVLSTDNSSR
                     IYTASELKRPVPIKNMSLSDLK"
     gene            5338..6510
                     /locus_tag="PK641_00860"
     CDS             5338..6510
                     /locus_tag="PK641_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HPG54940.1"
                     /translation="MMKYNFDELIDRHNTNAYKLELCKKVFGSTDLLPLWVADMDFRT
                     PDFILDAIKKRCEHPILGYTVQSDVFYDDLQKWLLNQHNWKVEKQHMGFLGGIVPGIA
                     YAVNALTNPDEEILVQTPVYPPFFTTPMHNGRKILFNQLIEKDGLYEMDFEGLESLIT
                     DKTKLMILCNPHNPGGRVWDKETLLKIARICHKHGIIVVSDEIHSDMCLKGYNHIPFA
                     SVSDEASSCSITFVAPTKTFNMPGLASSAYIISNSDLRARFFDFLYKMEQTVTLNPIT
                     MAATHAAYSLGEEWRRQMLDYVEANLDYVISEMAVKMPLIKPMRPQASFLLWLDFSAY
                     GLSSNELQLKIVNEAKLALNKGTSFGLGGEQYFRMNVATPRSVLEEAIKRLEKVFA"
     gene            complement(6575..6648)
                     /locus_tag="PK641_00865"
     tRNA            complement(6575..6648)
                     /locus_tag="PK641_00865"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6612..6614),aa:Asp,seq:gtc)
     gene            complement(6747..7304)
                     /locus_tag="PK641_00870"
     CDS             complement(6747..7304)
                     /locus_tag="PK641_00870"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HPG54941.1"
                     /translation="MLIKDEYINDIHFLVWKMEESELSARDQEKQLGKKLIEILCKED
                     VIIDHLTSGKPFVKNKQCEISISHTKKYIAAAIHDDNPIGIDIEYKGERVLRIQERFM
                     NKCEIETARSTNQLTECDYSLLCWCAKEAMYKKAGLHGIDFREDLYVHDAENGNVKVL
                     IRKDDINQDLLFHYENTDEFFIVVG"
     gene            complement(7309..7872)
                     /gene="gldD"
                     /locus_tag="PK641_00875"
     CDS             complement(7309..7872)
                     /gene="gldD"
                     /locus_tag="PK641_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HPG54942.1"
                     /translation="MKKNKYIIIAIFSLLMFSSCQQYTPLPRAYFRIKLNEKQYTKSD
                     TTKPYIFEYPSNIARIVTRNNNEWFDIVYPQYNARIYCSYTPIKNNFYEISEDSRTFV
                     YKHSIKADAITEQAFENTNNDVYGILYEIRGNTASSVQFVLTDSTKNFLRGALYFNNR
                     PNKDSLAPVINYIKDDVIRIMETTKWQ"
     gene            complement(7859..9151)
                     /gene="gldE"
                     /locus_tag="PK641_00880"
     CDS             complement(7859..9151)
                     /gene="gldE"
                     /locus_tag="PK641_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008016875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HPG54943.1"
                     /translation="MIVNIVLACVIILLMLCSAFASASEMAYFSLRPKDIEALENRNT
                     PTDQLVLKLRESPDRLLSSILITNNLVNVGIIILISKLMLNLFDFSSRPILAFLVETV
                     LITFILLLFGENMPKIYAANYSLKFVRFAAKPLHIIEKLLYPITWLMVKPAKRVKRFE
                     KHSKQAITINDLSQAFEITSDEISEDKEILEGIIKFGDISAASAMTARVDVIAIDDTA
                     TFQEIIDLINTNEYSRMPVFHENIDDIRGILYIKDLLSHINEPNYAWQKLIRKAYFVP
                     QTKHINVLLEDFQKNKTHIAIVVDEFGGTAGIITMEDILEEIVGEIDDEYDDNEQLYA
                     RLNENNYLFEAKILLNDFFKIEGINKQDFEKIIGEAETLAGLILELKGEIPVQETEIT
                     YKNYHFKIVSADKRRIKSIKLSILPKTEPEDEQEYEEE"
     gene            9499..9924
                     /locus_tag="PK641_00885"
     CDS             9499..9924
                     /locus_tag="PK641_00885"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HPG54944.1"
                     /translation="MFIIKNEIQFDTAHFLSGYEGKCANIHGHRYRLIVSIRCETLHK
                     EGHLRGMVDDFGSIKSILKEVELFFDHKMLVEDNEDGRKVKESLCALPNNFAVLCLPF
                     RPTCEEMARYIYRMLHQKGLAVSEVELFETPTNSCIYFE"
     gene            9924..10580
                     /gene="queE"
                     /locus_tag="PK641_00890"
     CDS             9924..10580
                     /gene="queE"
                     /locus_tag="PK641_00890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HPG54945.1"
                     /translation="MMEFNVIEKFVSIDGEGPTAGSLSVFIRFANCNLRCVWCDTSYA
                     WECEPQTEKLTAVEIVKYVKQTGVKHVTLTGGEPLIQEGLISLLAMLSDYEIHIETNG
                     SLDIHKFRLGDNVHFVVDYKLPDSGMEDKMYLYNLTAVKSTDAYKFVIASKKDLVKAI
                     EIVRAYRLDEITQVYFSAVFGKMDPSDIVEVMKEEHLNRIKLQLQMHKFIWNKNKRGV
                     "
     gene            10584..11174
                     /gene="folE"
                     /locus_tag="PK641_00895"
     CDS             10584..11174
                     /gene="folE"
                     /locus_tag="PK641_00895"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HPG54946.1"
                     /translation="MDKEKIAYHIREILVTIGEDPEREGLRETPERVARMYEEMFRGI
                     AYTNAEIAEKYAKTFDEEDMCTAKQGNFVLVSDIPIFSFCEHHMALMYNMKVSVIYRP
                     VDKIIGLSKIARIAEMVSHRLQLQERIGTDIAEIMTLSTGSNDVGVLIEGEHSCMTAR
                     GIKKPGAKTITTAFKGAFETNDSLRREALMLLGKLE"
     gene            complement(11207..13489)
                     /locus_tag="PK641_00900"
     CDS             complement(11207..13489)
                     /locus_tag="PK641_00900"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HPG54947.1"
                     /translation="MKKTWFLCLCSFCCSTIYIHAGTPITHPSQAICEGSSVTLTATT
                     LGDSYLWTPNGETTKSITVQPANNTDYKCTITKNGSSSQTGNLITAGGFEFLPSSPIT
                     SAQNKLGDWINYQYLNFDQGGVNINIGACTTSQNANNVKPSYFSNLAPHSGNYLFVCD
                     GGNSSDARVWQARNLKLKSGVEYQFSCFAANIDSEYLIHGASSLAKLKFVIENQDGTS
                     DLLQFTVPTTLGQWQEYKATYTPSTDMSWCHIYIVNFTTEPAGNDFAIDDIYFGTTIN
                     TQDDITDEFFRVNVDKKPTVSINDQNVCPNDNVTLSASIVGTYTNISWLENSTAMTDH
                     TTSITAKAPSVIGQSNLYDIFVTNGTCSAVNASASVKAIDCNVETVITHTINVCQNDT
                     LPITITANTVTGTFLWDTGETTESIQVPINEPSSSHTCTIIDNSQITREVFNISTEDC
                     HSEKSSTFCMTDCIKLSIIRDYDDPSDITFIWNTDPTNNDSTLIVCPTEINNTYTCQV
                     VHNDNYNSRELVTFNITLEDCSETESLTHDTIFCEGNDVVLSSLYPAMADSYNWSDGS
                     TESSITVSPQSETIYTCVTFIDTNYGYTLYETFNVQPVSNPIIDNIETTSTQADIQII
                     NGTAPFDYIVDDKHYTSQTIDDLTLGIHNVRIVDANGCSTIGDFELVSHEIEPMVFFS
                     PNGDNLNDRWLVKGIEFYPNAIIQIFDRFNKQLAKYNGSDPGWDGTYEGHGVPTTDYW
                     YLITIEQLSKQYSGHFILKR"
BASE COUNT         4535 a         2080 c         2526 g         4642 t
ORIGIN      
        1 ataacgggct tgagttcact gaacataaat atatagccaa aatgcttgat actaaaatat
       61 ttttcgctca tccctactgc tcttgggaaa aagtaaccat tgaaaataca aacaaattaa
      121 taagacagta tattcctaag aaattaaatt ttactgatcc taaatcagct ttctttaaaa
      181 atattcgtaa tttcgcactt gctacttgaa tctatataat ttgtattttt ctgtatttgc
      241 agtatttttt aatcttactt caaaaaatga aacgaattgt tgtaccttta ttcataatga
      301 ctattttctt aggatactcc caaattcctg ataaacgcgt aattatttcc aatgtttcgc
      361 atgataatat ttttgcggat attgagttaa aaactcttga tcctgaatca gcatatgcga
      421 accttccaga tggtgaaatt gattgcataa agggtaatac atggatattt aacatagaaa
      481 aacccaagta tgtacgtttt tctgctaatt cggatggcaa ttggatagat caattcgctg
      541 ttatttttcc tggtgactcc gtatcataca aaatagagct tgttggagaa acagattttt
      601 cgcacagtac gaaaaatttt aagaaagaat atcgtatgct attttcgggt aaagatgcgg
      661 caaactataa ttatagttat gatataagaa ctttatattc ggatactaat tcttataaaa
      721 gaccatggtt ccaaggtggt atggacttga tgaaataccg agaaggaatc ttagccgcct
      781 atgaatcgga aaaaaagttt ttatccaatt ataacgcaga acaccatctg tctgatgcat
      841 tttatcgcta tcagcttgac agaatgaaaa atatatgtat tgtttccctg tattctccat
      901 tttgttctaa ttataattta catattcctg aatcctattt tggtaatatg gaaattgtag
      961 aggatggtac tgatgcttat tacaaagcac tgcaaacgaa atatattgat tcacaaccac
     1021 ctgattttga cagaacatat caatatatta tccaaaatgc ccctaaaaag ttgcacgaat
     1081 atttaattac ctccttagta gaatgctatg cgtggagaaa aattggtgtg ccctcgttgg
     1141 aaaagattaa aagcgagatt caaaatccgg tctatcttgc acgtattgaa gaaaatgaaa
     1201 aaaagttaaa agaatatttc agaattcctc ttgatgatta tcctcaagaa ggaaagcctt
     1261 tttctgatta tttactcgaa aatacttatt ttcgttcgta tgacaatgca aaaaaaataa
     1321 cactaaagga gttgctaaat acatatcaaa ataaggctat ttatatagat atttgggcaa
     1381 gttggtgcat gccatgccgt aaagatatta gagaatcggt tgcaagtaaa gaatatttca
     1441 aagaaaaaaa agatcaaata gtgaagattt atacctcaat tgacagctcg gttgaagatt
     1501 ggctgagtgc atctgaacaa gaaggagtaa tacaaaatca atacttgtgt gagggtggtt
     1561 atgacgcact tctttatcag gttttaaaga gatctggaaa tggcattcca aggtatattc
     1621 tactaaataa agaacacaat atagagttgt taaatgctcc acgaccagtt caatttgaag
     1681 gtctctcggg tggatttgaa gatttgaaaa aaaacattga ccgaatgtta gaaagttcaa
     1741 aatgataatt tgaagataca aaataaaaat aagatgaatt ttgtacaaac tttatatatg
     1801 tcgcccgata acaatctttt tcatgattct tttggttggg tagcacttga atatcattta
     1861 atgagttggg cattgagttg tttgctgcta aaacggtcat atacaaatgt gaaactatac
     1921 gctaatgcag aggtcgccaa atgtttaaca gattatttaa aattaccata tgacgaagta
     1981 catattttat ctgacaaatt taaattacct catccctctt tgtgggcttt gccaaaattg
     2041 tggacataca gtctacaaga ggagcctttt ttacatgtag acgcacttct attttgaaag
     2101 tgcgaagttg tgaaaattta aaaattctaa gaaaagtaaa aagaaaaatt atgcacttgt
     2161 ttacatatta gaatgacttt gtcaaaatga acttgcaatt ggtataagca taaaaataat
     2221 tgcttaaata taaggtttaa atctaactta cattatcttt gcaagagaac aattaatact
     2281 gtttgctatg aaaagattat tgtcagtcgt atcatttttt ttaatatttt tagtagtttc
     2341 tgctcaagtt aaaacaatag agtatgctca aagagatact tgtaaactgt atatggatgt
     2401 atacaaccct aaaggacaat ctgatggaat gtgcgtgata tacgcgtttg gaggagcttt
     2461 taaaatgggc gataaaacag ataaggtaac ggtatcgtac atggaaaaat tgtcgcaaaa
     2521 tggaataacg gcaatagcaa tcaattatcg tttaggaatg aagggagatg cttacgaatt
     2581 aaaaggaatg aaattattga accgatatgc agatgctata cgcatggcaa cagaagatat
     2641 gctgacagca tccagatatg ttaatgatca tgcattagag ttgggagtag atcctaataa
     2701 aatagttttg tcaggatcta gtgccggtgc tatgacagca ttacaatgcg attttgaatt
     2761 atcaaataat actcaattag gacaaataat gcctgatggt tttcgtttgg caggagttgt
     2821 tacctttgcc ggtgcagtaa gtgtcttaaa tggtaaaatt gaatataaaa aagctcctgc
     2881 tccgactttg tttttccatg gaacggaaga tgctcttgtt ccatataata aaataagcat
     2941 gtttggaaaa ggaatgtacg gatcggcagc actagcaaag attttcaaaa agcatgaata
     3001 tccgttcggt ataataagat atgaacattt cggtcatgaa atagcatcta ttcctataca
     3061 cgaaaatatt tctgaaatac tacgtttttt agatacttat gtacgtgata gaaaagcagc
     3121 aaatttcgat ttgactatac acgaaactgc agtaacaaaa cctatgctta atttttcaat
     3181 tcgctcaatt aataaaatgc aagaaattaa aatttctaaa gaagcgcaaa gtcgaatgag
     3241 tggaggtgaa tcaagataat aaacatttaa ttcatgaaaa aaacacctaa atataaaata
     3301 ggattagctc ttagtggagg tggtgttaag ggatttgctc atgccggtgc atttaaagca
     3361 ttggaagagt atggaataaa acctcaaata atttcaggaa caagtgcagg atctgttgcc
     3421 ggggttttgt attcttcagg atataagcct caagaaattt acaaaatgtt ttttagtaag
     3481 ggggttaata attttgtagc actaacattg cctgtatcgg gtttttttaa tccatcaaag
     3541 tttgttcagt ttcttcgaaa gcaaattaaa tacgaaaatt tggaagacct acccatacct
     3601 gtaagagttg tggctaccga tttggatcat ggtaaaagtg ttgtatttac taaagggcgt
     3661 cttgcagaaa gagtcatggc atcatcaacc atacctattg tttttgtgcc tacagaaata
     3721 gatggtgtac actatgttga tggtggaata ttttgcaatt ttccggtaag tgcaatacga
     3781 gaagattgcg aaaaagttat aggagtaaat gtaagtccat tagtgccgac agaatataaa
     3841 cagacattgg taggaattgc agagcgctcg tacaatttta tgtttagagc aaatactatt
     3901 gaggagtgta aaaaatgtga tattatcata gaagttgcag atgctttaaa atacagtaat
     3961 ttagatttaa gcaaagtaga ggatatttat cgtttgggtt acgagtcaat ggtgcaggaa
     4021 ttggaaaaaa ataaagatat tctggatgat tttcgataaa ctaatttcta tatgattaga
     4081 agaatattta taacactatt ttttgtgtgc atcttttcaa gtgcatacgc aacgataatt
     4141 aacggaatcg ttaaagacaa acaatctgta ccggtttcat ttgccagtgt ttaccttaat
     4201 aataatccaa ctgtaggtac aataacagat tctgagggac atttttctct cgaaatagag
     4261 aactttaagt ctgatgtttt gattatttcg tttattggtt atgaaactat aagatatcct
     4321 ttgtctgatt tgaagggcaa tacaaatttg tcttttacct taaaggagca gcctatattg
     4381 ttgcaagaaa cattagtttc agcaaaaaat ccttcaaaaa aaatgacaaa aagggataag
     4441 aggaatatac ttaaggccgt aagaaaacga atggatatag attttcctaa tcaatcatgc
     4501 gggtacaaaa tagtttctga ttatattatc tacaatcaag atgatgttgt tgcttttgaa
     4561 gagttggttg gtactttggt tgaaattcct tctttgaaac gtaaaaacgg acgtgattcg
     4621 atacaattga aaggtgactt atgtaagcga tatcgaaatc aacagatgga aagtaaattt
     4681 aataatttaa gtgctaatgg actaaacgat aaatataaac aagtagcagg aagggttgat
     4741 tctgttgtga tgattcatag cacgatgtgg ggtagtgaaa ttcaatggtt gtttgatgaa
     4801 tttattggaa atacctcaaa atggactttt acagacaaag gagactatac tgtgcttaat
     4861 tatttcgata ggcgaaatta tttagggata ttgattattg aacttaattt aaactatgtg
     4921 attgattcat attcgtatag tgtgcaaaaa atatcacaag acatgactat taaggcaaat
     4981 attccatttg gatataaatt gtctaaagaa caattagctt tatttaatgt gtttaatata
     5041 ggcgaaaatc aaatagaaaa atttagatta aaaacagttg atactcacat caaacgaaat
     5101 attatatata aaaatactga tacacgtgtg gttgtgtcgg aaaagaatgt cgaactaaaa
     5161 tcaaaaatgg cggataaaaa cggcaaagga atgaatgttc acagtacagc cgcaataaaa
     5221 gtattgtcaa ccgacaattc atcgcgaata tatactgctt cagaattgaa acgcccggtt
     5281 ccgattaaaa acatgagttt gtctgactta aaatgatggt ttgtaaatat tatttagatt
     5341 atgaaataca attttgatga gttaattgat agacataata caaatgctta taaattagaa
     5401 ttgtgtaaaa aagtgtttgg ttcaactgac cttctgcctt tatgggtagc agatatggat
     5461 ttcagaacac ctgattttat tttagatgct ataaaaaaac gttgtgagca tcctatttta
     5521 ggatacacgg tgcaaagtga tgttttctat gatgatttac aaaaatggtt gttgaatcaa
     5581 cataattgga aagttgaaaa acaacatatg ggattcttgg gtggaatagt tcccggaata
     5641 gcatacgcag taaatgcttt gacaaatcct gatgaagaga tattagttca aactcctgta
     5701 tatcccccct tctttacaac tcctatgcac aatggtagga aaattttgtt caatcaattg
     5761 atagagaaag atgggcttta tgaaatggat tttgaaggct tggaatcact gataaccgac
     5821 aagacaaaac ttatgatatt atgcaatccg cataaccctg gtggaagagt ttgggataaa
     5881 gaaactttac taaaaattgc tcggatatgt cataagcatg gaattattgt agtgtctgat
     5941 gagattcatt cggatatgtg tttgaaggga tacaatcata ttccttttgc gtcagtttct
     6001 gatgaagcat catcatgctc tataacattt gtagctccta ctaaaacatt taatatgccc
     6061 ggattggcaa gttctgcata tatcatatct aattctgatt tgagagctcg tttttttgac
     6121 ttcctatata aaatggagca aactgttaca ttaaatccga taaccatggc ggctactcat
     6181 gctgcttatt cgttgggtga agaatggaga aggcagatgt tagattatgt agaggctaat
     6241 ttagattatg taatatccga aatggctgtt aagatgcctt taattaaacc gatgagaccg
     6301 caagcatcat tcttattgtg gttagatttt tctgcctatg gcttgtcttc taatgaatta
     6361 caacttaaaa ttgttaatga agctaagctt gctcttaata aagggacttc attcggctta
     6421 ggaggtgagc aatattttcg tatgaatgta gctactccac gttctgtttt ggaagaggct
     6481 ataaagcgtt tggagaaagt tttcgcttaa ttttttatat aggtgtaaat gcaaaaaagt
     6541 cagtaaaatt atatttactg acttttaatt tttgcggtgt ggatggggct cgaacccacg
     6601 acctccgccg tgacagggcg gcattctaac cagctgaact accacaccaa acagcttgat
     6661 ttctcaaatg cgatgcaaag ataagacaaa taatttatat acaaaatact agatgctgtt
     6721 ttttttgaat tttatatcaa aaaaaatcag ccaactacaa taaagaactc gtctgtattt
     6781 tcgtaatgga atagaagatc ttgattaata tcatctttac gaattaaaac ttttacatta
     6841 ccattctcgg catcatgaac ataaaggtct tctctaaagt caattccatg taatccggct
     6901 tttttataca ttgcttcctt ggcacaccaa cataaaaggc tataatcaca ttctgttaat
     6961 tgatttgtgc tacgtgctgt ttcaatctcg catttgttca taaaacgttc ttgtatgcgc
     7021 agtacccgtt cgcctttgta ttcaatgtct attcctatag gattatcatc atgtatggct
     7081 gcagcaatat attttttagt atgacttatg ctgatttcgc attgtttgtt ttttacaaaa
     7141 ggctttcctg atgttagatg atcaattatc acatcctcct tgcacagaat ttctattagt
     7201 ttttttccta attgtttttc ttgatctctg gcactcaatt ctgactcttc cattttccat
     7261 acgaggaaat ggatatcatt gatatattcg tcttttatca acatttaatt actgccattt
     7321 tgtggtttcc atgatgcgaa ttacatcatc ttttatgtag ttaataacag gtgccaatga
     7381 atctttattt ggtctgttat taaaatacaa ggctcctctt agaaaattct ttgtactatc
     7441 tgttaaaaca aattgtaccg aagatgcagt atttcctctt atctcgtata aaatgccata
     7501 aacatcatta ttagtattct caaaagcttg ctccgttatt gcatcagcct ttattgagtg
     7561 tttgtatacg aaagttcgtg aatcctcgct tatttcataa aaattatttt tgataggagt
     7621 gtagctgcaa taaatacgag cattgtattg cggataaact atgtcgaacc attcattatt
     7681 attgcgagtt actatgcgag caatatttga tggatattca aaaatgtatg gtttagtagt
     7741 atccgatttt gtgtattgtt tttcgtttag ttttattcga aaataagctc ttggcagtgg
     7801 ggtatattgc tggcatgaac taaacatcaa taaagaaaat atggctatga ttatatattt
     7861 attcttcttc atattcttgc tcatcttcag gttcagtttt gggcagtatg cttaatttga
     7921 tgcttttaat acgacgcttg tcggcagata cgattttaaa gtgataattt ttgtatgtaa
     7981 tttctgtttc ttgaaccgga atttcaccct ttaactccaa gattagaccg gctaatgttt
     8041 cggcttcccc aataatcttt tcaaaatctt gtttattaat gccctctatt ttaaagaaat
     8101 catttaataa tatttttgct tcaaataagt aattattttc gttgagtctg gcatataatt
     8161 gttcattgtc atcgtattca tcatcaattt cgccgactat ttcttccaat atatcttcca
     8221 ttgtaattat accggctgta cctccaaact catcaaccac aatggcaata tgagttttgt
     8281 ttttctggaa atcttccaaa agtacattaa tgtgttttgt ctgtggtaca aaatatgctt
     8341 ttcttatcaa tttttgccat gcataattag gctcgtttat atgcgaaagt aaatctttaa
     8401 tatacaatat gccacgtata tcatcaatat tttcatggaa tacaggcatc ctggaatatt
     8461 cattagtgtt gattaaatca attatttcct gaaatgtggc tgtatcatct attgcaataa
     8521 catcaactct tgccgtcatt gctgaggctg cacttatgtc tccaaatttg atgataccct
     8581 ctaaaatttc cttgtcctca ctaatttcat cagaggtaat ttcgaaagcc tgtgacaaat
     8641 cgttaattgt aatagcttgt ttactatgct tttcaaaacg ttttactctt tttgcaggct
     8701 ttaccattaa ccatgttata gggtataata atttttcgat tatgtgtaag ggtttagccg
     8761 cgaaacgaac aaattttaga gaataatttg ctgcgtatat tttaggcata ttctctccaa
     8821 acagtagtaa aataaatgtg attagaactg tttcaaccaa gaatgccaat attggtctac
     8881 tcgaaaaatc aaacaagttt agcatcaatt ttgatattag aataatgatg ccaacattta
     8941 ccaagttatt ggtaattaag attgaagaca gtaatctatc cggcgattct cttaatttga
     9001 gaactaattg atcggtaggc gtatttctat tttctaaagc ctctatgtct ttgggcctta
     9061 gtgagaaata agccatttct gatgctgatg caaatgcaga gcacagcatc agtaaaataa
     9121 tcacacaagc aaggactata tttactatca tatttgaata ttcagttaca actgcaaaaa
     9181 tacaatatta aattgatagt aaaaaagaac aatgcaagct cttgaaaatt tatcgatgct
     9241 agtgcatcat gcagagtcta ttactttgcg tcatactgtc ggttgcattt atcctcttga
     9301 attcagtatt tacgatgcaa agtggaatct gaatcacgaa tattagaata tttgcaatag
     9361 gctttaaaat ttttcgtaac tttgcagtcc gacagttcgt ttagaccatc ctgtcgttaa
     9421 aaaaaaccag gcttgtaacg tgaatgtttg tttcgcaact tacaaggtgg tgaaacaaac
     9481 atttttattt gtagtagtat gtttattatt aagaacgaaa ttcaatttga cacagctcat
     9541 tttttgagcg gatacgaagg taaatgtgcc aatattcatg gacatcgtta tagattgatt
     9601 gtatctatac gctgcgaaac attacacaag gagggtcatt taagaggtat ggttgatgat
     9661 tttggttcta ttaaaagtat attgaaagag gttgaactat tttttgacca taaaatgtta
     9721 gttgaagata acgaagacgg acgtaaagta aaagaatctt tgtgtgcatt gccaaataat
     9781 ttcgctgtgt tgtgtttgcc attcagaccg acatgcgaag aaatggcgcg ctatatttac
     9841 agaatgttgc atcaaaaagg tttggctgtt agtgaggttg aattatttga aaccccaaca
     9901 aatagttgta tatattttga gtgatgatgg aatttaatgt tatagaaaaa tttgtatcaa
     9961 tagacggtga gggccctaca gcaggatctt tgtcggtatt catacgtttt gccaattgta
    10021 atttgagatg cgtttggtgt gatacttctt atgcttggga gtgtgagcct caaacagaaa
    10081 aactcaccgc tgtagaaata gtcaaatatg ttaaacaaac aggagttaaa catgtaactt
    10141 tgaccggtgg agaacctttg atacaagaag gtttgataag tcttttggct atgctttctg
    10201 attacgagat tcatattgaa acaaatggct cattagatat tcataaattt agattaggtg
    10261 ataatgtgca ttttgttgtc gattataagt tgcctgacag tggcatggaa gataaaatgt
    10321 atttgtataa tttaactgct gttaaatcta cagatgctta caagtttgta atcgcttcaa
    10381 agaaagattt ggtaaaagca atagaaatag ttagagcata tcgtttagat gaaattactc
    10441 aagtgtattt tagcgctgtt tttggtaaaa tggacccttc tgatatagtt gaagtgatga
    10501 aagaagaaca cttaaataga attaagttgc aattacaaat gcataaattt atatggaaca
    10561 aaaataaaag gggagtataa aaaatggata aagaaaagat tgcatatcac attcgagaaa
    10621 tccttgtaac tataggtgaa gatccggaac gtgaaggatt acgcgaaaca cccgaaagag
    10681 tagctcgcat gtacgaagaa atgtttaggg gaattgcata cactaatgcc gaaattgcag
    10741 aaaaatatgc taaaaccttt gatgaggaag atatgtgtac tgcaaaacaa ggtaattttg
    10801 tattagtgtc agacattcct atttttagtt tttgtgagca tcatatggct ttaatgtaca
    10861 acatgaaagt ttccgttata tatcgtcctg tggataagat aataggctta agtaagattg
    10921 cgcgtattgc tgagatggta tcgcatcgct tgcaactgca agaacgaata ggaacagata
    10981 ttgcagaaat tatgacacta tcaacaggaa gcaatgatgt gggtgtgttg attgaaggtg
    11041 aacatagttg tatgactgca aggggtataa aaaagcccgg agcaaaaact ataaccacag
    11101 catttaaagg tgcttttgag acgaacgact cacttcgtag agaagcgctt atgctattag
    11161 gcaaattaga ataaaaaaat cgggaaataa attttcccga ttttttttat cgcttaagta
    11221 taaagtgtcc ggaatactgt ttgcttagct gttcaatagt tattagatac caataatctg
    11281 tcgtaggtac accatgtcct tcgtatgttc catcccaacc cggatcggat ccattgtatt
    11341 tagcaagctg tttattgaat ctgtcgaaaa tttgaattat agcgttaggg taaaattcaa
    11401 tacccttaac taaccatctg tcgttcagat tgtctccatt tggtgagaaa aataccatag
    11461 gttcaatttc atgcgaaact aattcaaaat ctcctattgt agaacaccca tttgcatcaa
    11521 ctatacgtac attatgtata cctaatgtca aatcatctat tgtctgactt gtataatgtt
    11581 tatcatcaac tatgtagtca aatggagctg taccattaat aatttggata tcagcttgtg
    11641 ttgatgtagt ctcaatattg tcaattatag gattagatac aggttgtaca ttaaacgttt
    11701 cgtacaaagt gtagccataa ttagtatcta taaaagttac gcaggtgtaa atggtttcgc
    11761 tttgtggact tactgtaata ctgctttcgg ttgagccgtc agaccagttg taactatctg
    11821 ccattgcagg atacaaagac gataatacaa catcattccc ttcgcaaaat atcgtatcgt
    11881 gagttaatga ctctgtttca gaacaatctt ctaatgtaat attaaatgtt accagttcgc
    11941 gcgaattata attatcgtta tgtactacct ggcaggtgta agtattattt atttcagttg
    12001 ggcaaacaat aagagtagaa tcattatttg tcggatctgt gttccatata aaagtaatgt
    12061 ccgaaggatc atcatagtct cttattattg acaatttaat gcaatctgtc atgcagaatg
    12121 tgcttgattt ttctgaatgg caatcttctg tagatatatt aaaaacttct cttgttattt
    12181 gtgagttgtc gataattgta catgtgtgag atgagcttgg ctcatttatt ggtacttgaa
    12241 ttgactctgt tgtttcgccg gtatcccaca aaaaagtacc agtgacagtg tttgccgtaa
    12301 tagtaattgg caaagtgtca ttttgacaca cattaatagt atgtgttata accgtttcta
    12361 cattgcaatc tatagccttg actgatgcag atgcgttaac cgcagagcaa gttccattag
    12421 ttacgaaaat atcatataaa ttgctttgac caattacact aggagctttt gctgtgatgc
    12481 tagttgtatg atcagtcatt gcagttgaat tttcaagcca agatatattt gtatatgttc
    12541 ctacaattga agctgacagt gtaacattat cgtttggaca aacattttgg tcattaatac
    12601 tgacagtcgg ttttttatca acatttaccc tgaaaaattc atctgttatg tcatcttgag
    12661 tatttattgt tgtcccaaaa tatatatcgt caattgcaaa atcgttacct gcaggctccg
    12721 tagtgaaatt tacaatgtat atatgacacc aactcatatc tgttgatggt gtatatgtag
    12781 ctttatactc ctgccattgt cctaatgttg ttggaactgt gaattgtagt aagtcactgg
    12841 ttccatcttg attttctata acaaatttaa gttttgcaag gcttgatgct ccatgaatca
    12901 aatattctga gtctatattt gcagcaaaac agctaaactg gtattcaact ccacttttta
    12961 gttttaaatt acgagcttgc catacgcgtg cgtcagagct gttgcctcca tcacacacaa
    13021 ataaatagtt tccggaatgt ggtgccaaat ttgaaaaata tgaaggtttt acattgtttg
    13081 cattttgcga tgtagtacat gcacctatgt tgatattaac gccaccttgg tcaaaattca
    13141 agtattggta attaatccag tcgcctaatt tattttgtgc cgatgtgata ggagagctag
    13201 ggagaaattc gaatccaccg gctgtaatta aattacctgt ctgcgaacta gaaccgttct
    13261 ttgtaatcgt acacttgtag tcggtgttat tagcaggttg tacagtaatg ctttttgtgg
    13321 tttctccatt tggtgtccat aagtatgaat ctcctaaagt tgttgctgta agagtaactg
    13381 aagaaccttc gcatattgct tgtgaggggt gtgtaatagg tgtaccggcg tgtatgtata
    13441 tggttgagca gcagaaactg cacaaacata aaaaccaagt ttttttcata tgtttcgata
    13501 ttaggttcgc aaatataatc attattttaa aatagcaaaa actttatgta gtatcagatt
    13561 agtgcataaa gccttttatg tcagaaataa aagctaaaaa tatgcttgct atagttatta
    13621 ctaatatttt tatttcttct ttactggaac taaaaaaaca tcttggttaa tttcgctgtt
    13681 ataaatagtt ttacccaatt cgtaaatggt gtaattacca ttaggttcct ccatattcat
    13741 gattttcaaa agcttgagct tgtatgatat ggtgttaata taa
//
LOCUS       DAOPID010000010        13629 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_5268_length_13629_cov_4.472816, whole genome shotgun sequence.
ACCESSION   DAOPID010000010 DAOPID010000000
VERSION     DAOPID010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 13629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13629
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5268_length_13629_cov_4.472816"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PK641_00905"
     CDS             complement(<1..418)
                     /locus_tag="PK641_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPG54948.1"
                     /translation="MMNINGKDISKQEFEYFYHKNNSNTSSNYISLDEYVDLFINFKL
                     KVADAYEQKLDTAKSFKDEFNSYRNQLIRPYLTDDSIKEVLVKEAYSRLKEDIEVNHI
                     LFRIENQNDTIAAYEKAMSAKKRINKTKKHPTSVFCF"
     gene            complement(485..1960)
                     /gene="guaB"
                     /locus_tag="PK641_00910"
     CDS             complement(485..1960)
                     /gene="guaB"
                     /locus_tag="PK641_00910"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPG54949.1"
                     /translation="MSFIADKIVMDGLTFDDVLLIPAYSEILPRDVDLTTRFSRNIEL
                     KTPIVSAAMDTVTEAKLAIAIAREGGIGVIHKNMSIVEQAKQVHSVKRAENGMIYDPI
                     TIRREKQVADALALMSEYKIGGIPVVDENKKLVGIVTNRDLRFERNLQRPIEEVMTKE
                     NLVTTNQSADLEAAAQILQNHKIEKLPVVDKDGKLVGLVTYKDITKAKDKPMACKDSK
                     GRLRVAAGVGVTADTFDRMSALVDAGVDAIIIDTAHGHSKGVIEILKEAKKRFPQIDI
                     VVGNIATGAAAKALVEAGADAVKVGIGPGSICTTRVIAGVGVPQLSAIYDVAKALKGT
                     GVPLIGDGGIRYSGDIVKALAAGASCIMAGSLLAGVEESPGDTIIYNGRKFKSYRGMG
                     SLEAMQKGSKDRYFQDMEDDIKKLVPEGIAARVPFKGSLYEVIYQLVGGLRAGMGYCG
                     ASNIEKLHNAKFTRITTAGVKENHPHDVTITQEAPNYSRGE"
     gene            2187..3338
                     /locus_tag="PK641_00915"
     CDS             2187..3338
                     /locus_tag="PK641_00915"
                     /inference="COORDINATES: protein motif:HMM:NF040309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6051 family protein"
                     /protein_id="HPG54950.1"
                     /translation="MICVSDVRVNQRTDILKKIFTKEESCRIEDSAVDISFFEFKERG
                     NDSNVRELQHSFLIPSFIKTSDDAVNENSSFRYPMVRPPHHNENACILLFHGLNERSW
                     DKHAIWAETLARMTGKPVILFPIAFHINRTPSSWYNPRVLMHWVADRRKRTASAENVT
                     FANLALSSRLSSSPLRFYTSGRETCYDVAQLMDNIKAGKHPYLNENTKIDIFAYSIGA
                     LMSQIMLLSNYKNMFENARLFMFCGGSIFNMMNGNSPYIMDSQAFLDMIAYYQNDHLF
                     QAINNDDPFDLPFKSMIDANYYTDYRTDFFNKAQNRIQIISLAKDKVIPYKGICAALG
                     AEAAAKCVIKLDYPFEYSHEMPFPTKMNATNEVDKMFMDVFGRAANFLS"
     gene            complement(3343..4254)
                     /locus_tag="PK641_00920"
     CDS             complement(3343..4254)
                     /locus_tag="PK641_00920"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HPG54951.1"
                     /translation="MNFELTILGSGSAIPSFGRFSTSQVLSTHNKSFMIDCADGTQFR
                     MRELGINITRMGHIFISHLHGDHCFGLMAVISTMSMMHRTADLYIHSHPDLIELLQPQ
                     IDYFCDDLRYKVYFEPFNPKQSELIYEDRSLTVNTIPLVHSTPTCGFLFKEKPNQPHL
                     NKDLIDFFQIGVKDIKNIKEGADWIKPDGTIIHNADLLTPATKSKSYAYCSDTRYSER
                     IIPLIHGVDCLFHEATFEESEISRAKDTLHSTALQAANIAKKAEVGQLLIGHYSARYQ
                     NLNKLLAEARSVFPNTKLAEDKTHFQW"
     gene            complement(4251..6152)
                     /locus_tag="PK641_00925"
     CDS             complement(4251..6152)
                     /locus_tag="PK641_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HPG54952.1"
                     /translation="MNRTDYRSILKQYWGYDDFRSLQEDIVSSVGSGKDTLGLMPTGG
                     GKSITFQVPALSMDGLCLVITPLIALMKDQVDGLRKKEIKAAAIYSGMSYEEIMTTFN
                     NCIYGHYKFLYVSPERLGTRVFLEKVPQLDISMIAVDEAHCISQWGYDFRPAYLKIAE
                     IRKMLPDVPVLALTATATPEVVEDIQKQLQFAKNNVFKKSFQRENLAYVVQKTEDKLQ
                     KLLHILNSVNGSSVVYVRNRKKTKEVADFLNNNNISADYFHAGLPNSAKDNRQERWKS
                     GDCRVIVATNAFGMGIDKADVRTVIHIDLPDSIEAYFQEAGRAGRDGLKSYAVLLYEN
                     ADITKLKKRLSDNFPTKEQILRTYEYLAYYYQVGVESAEGCVFDFNLIDFCTKFKLSI
                     NPTHSALKILELAGYIEYTEEIESHTRIMFSMYRDELYKYDIPDKTDVVMKCLLRSYT
                     GLFADYVNIDEDLIAKRTKLDKSDVLKSLIDLSRLNVISYIPYKKTPYIVYTRQREHT
                     ERIIIGKDVYENRKERFENRIKAMSDYSTSDNACRSRILLAYFGEKDSDNCGICDFCL
                     KQKSNNKKTNNLDKKIIDLLKDGPQEISYLIGKLNCSEETIFTVIRQLMDEKTIATEN
                     KKTIYLIKK"
     gene            complement(6156..9449)
                     /gene="mfd"
                     /locus_tag="PK641_00930"
     CDS             complement(6156..9449)
                     /gene="mfd"
                     /locus_tag="PK641_00930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HPG54953.1"
                     /translation="MDSSDFLKILRQHPNFDTLQKWVSSSSKNLKINGFCGSSASIFL
                     HEAQIGHHLPAVYVLHDHEEAGYFYNDLIQFYGDENIFYFPSSYKKKVKDGILKDSAN
                     QVLRTETLNAISMRSDWTIVTYPEALSEKVIEPNKLEKETLHLRVGEKPGIEFVIEAL
                     TEYGFEQIDFVYEPGQFSVRGSIIDVFSYSNDLPYRIDFFGDEVDSIRNFDIESQLSE
                     DSQNEVSIISNTINTETKNLVPIFDFFPIETLFGLYDADYLSEVLQTIIEENTDNECF
                     ITAIEFEIRIKTYKIIEWGLRNHFGSRKTIQFNESIQPIFHKNFDLTASDFKQRQSEG
                     FEVYVLSDNSKQIERLKDIFEDREEHIIFIPVLSILHEGFIDNDLKICVYTDHQIFER
                     FQKYTLKSAKTRTGKVVLSLKELMQFQIGDYIVHTDHGVGKFGGLIRTEMGGKMQEVI
                     KLTFKDDDIIFVNIHSLHRISKYKSKDSEPPKLNKLGTAAWTNLKEKTKKKVKDIARE
                     LIRLYAERREEKGFSYSPDSYLQQELEASFIYEDTPDQSKATAVVKKDMESEKPMDRL
                     ICGDVGFGKTEIAIRAAFKAVSDNKQVAVLVPTTVLAFQHYKSFSRRLKRFPCNIDYL
                     SRARKPVETKKILEKTAAGRVDILIGTHKILGKEVKFKDLGLLIIDEEQKFGVSAKEK
                     LRQLRTNIDTLTLSATPIPRTLQFSLMGARDLSVINTPPPNRYPIQTELIPFDDDVIK
                     DAIEYEISRGGQVFFVNNRISNLPELEIHLRKLVPKARICVGHGQMDSEKLEEILINF
                     INGEYDILLATSIIESGVDIPNVNTMFINSAQHFGLSDLHQLRGRVGRTNKKAFCYLI
                     TPPLSSLPTDSRRRLQAIENFSDLGSGIHIAMQDLDIRGAGNILGGEQSGFISDLGYE
                     TYHKILDESIHELKHDEFADVFAEEIKEKDNIYATDCVFESDLELLFPSYYVQNISER
                     MDLYRRLDAIKEANALISFEKEMVDRFGEIPEVSQELIQVIRLRWLCMSLGIEKITLK
                     NNRLSAYFTNNFQSHYYQSEIFNKILQYAMVHHSNTKFKEQTDKRLISIDGVNGIRQA
                     YKCLEEMCL"
     gene            9621..10361
                     /locus_tag="PK641_00935"
     CDS             9621..10361
                     /locus_tag="PK641_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005648956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HPG54954.1"
                     /translation="MSDSIVLIPTYNEKENIENIIRAVFSLPKELHILVIDDGSPDGT
                     GAIVKRMQEEFPGSLHLMERSGKQGLGRAYIAGFKWSLSHGYGYIFEMDADFSHNPND
                     LLKLYAVCHDEGADMSIGSRYVSGVNVVNWPMGRVLMSYFASKYVRFVTGLPVADTTA
                     GFKCYKREVLETIELDKIRFVGYAFQIEMKFTAYKCGFIIKEVPIIFINRELGTSKMS
                     GGIFGEAVFGVLKLKWNSLFRKYPKKQA"
     gene            10372..11709
                     /locus_tag="PK641_00940"
     CDS             10372..11709
                     /locus_tag="PK641_00940"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HPG54955.1"
                     /translation="MYLIKDAHIVNEGRVFKASVLIKDDKIDSIFKADVPESIIQSAE
                     LIDASDCWLMPGIIDDHVHFRDPGFPEKADFYSETCAAIAGGVTSVMDMPNTNPQTTS
                     IHNWEDKMNIASDKSLCNYALYLGATNDNLSDLLQADPTKVCGIKLFLGSSTGNMLVE
                     KKDAIENIFAKSPLLIAVHAEDEEIIQKNRLHFTNLYADKIPIEFHTSIRSAEACFKA
                     SAMAVELAHKYNSRLHLAHISTEKELALFKNKSIDKKRISSEVCVNYLWFSYKDYEKY
                     GAKIKCNPSIKTETDKCALLNALSSDKIDVIATDHAPHLLSEKNGYCMQAVSGIPSVQ
                     FSLPLMMEFVKRGNISIEQLVTKMCHNPAKIFNIEKRGFIRKGYKADLTIVRRKELWR
                     ADQSCVLSKCAWSPYEGVEFTTKVAYTFVNGRKVFDNGKMSNTLRGEALCFNA"
     gene            complement(11744..12883)
                     /locus_tag="PK641_00945"
     CDS             complement(11744..12883)
                     /locus_tag="PK641_00945"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HPG54956.1"
                     /translation="MKKTLSINLNGIAFNIDEDAYQTLKQYISEISAHYNTDEDKEVI
                     KDIEARIAELFSEKLHAESKNVVEINHVNSVIDVLGRPNQFESEEANTETDNNTATKK
                     RNKHFYRDPENSMLGGVAAGLAVYLGWDVTLIRILIILTVIFGFGWSILLYIVVWVIA
                     PEARTAAQKLEMRGEDVTIENIKGQFNKAKDYVESDNFKSSAKSVGKTMGDIFITICK
                     VILIIVGCFAGFIGLIVIGALLTAAVCVFANPALFVGLLPFTVLPSYLATIIGICALL
                     IILCPLIGLVTICIRIIRHKNGSRSKWFGWTLLIVWLLSIITLVGISIGNINRLQEFG
                     SEWENWRNQANTTTIQWYSSYPNSYTIDVNDSIDNSTTLDSTAVF"
     gene            complement(12898..13227)
                     /locus_tag="PK641_00950"
     CDS             complement(12898..13227)
                     /locus_tag="PK641_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HPG54957.1"
                     /translation="MSTDNITSQMRKGFLEYCILLLINKKPYYTSDIIQELRSVKLIV
                     VEGTLYPLLTRLKNSGLLDYKWVESTQGPPRKYYALTENGKLFLKDLDGAWTDIKQVV
                     EFIKSNK"
     gene            complement(13419..>13629)
                     /gene="aroE"
                     /locus_tag="PK641_00955"
     CDS             complement(13419..>13629)
                     /gene="aroE"
                     /locus_tag="PK641_00955"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="AroE; catalyzes the conversion of shikimate to
                     3-dehydroshikimate; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HPG54958.1"
                     /translation="NVKACPEIPYEYITKDHLVYDLIYNPIRSLFLHQAELKGATIYN
                     GWEMFVYQALRSYKIWENKDWTHKF"
BASE COUNT         4140 a         2368 c         2281 g         4840 t
ORIGIN      
        1 taaaacagaa cacagaggtg gggtgcttct ttgttttgtt gattcttttt tttgcagaca
       61 tagccttttc gtatgcagca atcgtgtcat tctgattttc aatacgaaat aaaatgtggt
      121 taacttcaat gtcttctttt aaacgagaat atgcttcttt aactaatacc tcttttatgc
      181 tgtcgtctgt aagatatgga cgaatcaact ggttgcgata actattaaat tcgtctttga
      241 acgattttgc cgtatccagt ttttgctcat atgcatctgc aaccttaagt ttaaagttga
      301 tgaacaaatc tacgtattca tcaagtgata tgtaatttga acttgtattg gaattgtttt
      361 tgtgataaaa atattcaaat tcttgtttgc ttatatcttt accatttatg ttcatcacaa
      421 cttgtgcatt tgtgttaatc aaaaagaata tcgcgattgt gcaaaaaacg atttttttca
      481 tttgttattc tcctcttgaa tagtttggtg cttcttgtgt tattgttaca tcgtgtggat
      541 gattttcttt aactccggca gttgtgatgc gtgtaaattt agcattgtgc aatttttcta
      601 tattagaagc accgcagtat cccataccgg cacgtaatcc accaactaat tgatatatta
      661 cttcatataa agatcctttg aaaggaacac gtgcagcaat accttcagga actaattttt
      721 tgatatcatc ttccatatct tggaaataac gatctttaga acccttttgc atagcttcta
      781 gtgatcccat acctctatat gatttgaatt ttctgccatt atatataatg gtgtcacccg
      841 gagattcttc aactcctgct agtagtgaac ctgccataat acaacttgca cctgctgcca
      901 atgcctttac aatatcaccg gagtatctta taccaccgtc acctattaaa ggaactccag
      961 tgcctttaag tgcttttgct acatcatata ttgctgataa ttgaggaaca cctacacctg
     1021 caataacgcg tgttgtgcat atagatccag gtcctatacc tactttaaca gcatcagcac
     1081 ctgcttctac caatgcttta gctgcagctc ctgttgctat attacctaca actatatcta
     1141 tttgcgggaa acgcttttta gcttctttaa gaatctctat tacacctttt gaatgtccgt
     1201 gagcagtgtc aattatgata gcgtcaacac ctgcatcaac tagtgcagac atacgatcaa
     1261 aagtatctgc ggtaacacca actcctgcgg ctactcttag tctccctttt gagtctttgc
     1321 aagccatagg tttatctttg gcttttgtga tatctttata agtaactagt ccgactaact
     1381 taccgtcttt gtcaactaca ggtaatttct ctattttatg gttttgtaga atttgagcag
     1441 ctgcttccaa gtcggctgat tgatttgttg taaccaaatt ttctttggtc ataacttcct
     1501 ctattggacg ttgtaaattt ctttcgaagc gtaaatctct attggtaaca atacctacta
     1561 attttttatt ttcatcaact acaggtattc cgcctatttt atattcactc ataagagcaa
     1621 gagcatcagc aacctgtttt tctcttctta tggtaatagg atcatatatc atgccatttt
     1681 cagctctttt aacgctgtga acttgtttgg cttgctcaac aatagacata tttttgtgaa
     1741 taacacctat accaccctca cgcgcaattg caatggccaa tttcgcttct gtaaccgtgt
     1801 ccatggcagc agatacgata ggagttttta actcgatgtt acgcgaaaaa cgtgttgtta
     1861 aatctacatc acgcggtaat atctctgaat aagctggaat taaaaggacg tcatcaaaag
     1921 taagtccgtc cattacgatt ttgtctgcaa taaatgacat agtgtggcag gattaaaatt
     1981 tattgcgtgc aaatatacga aaatatcttt ccggatgttc tattgtgccg ttaaaatatg
     2041 ttggtattta ttataaaatt caccccataa ttaatgtttt aacaccacaa aaagttagtc
     2101 tgcaccaaat tgactttcaa ttaccacata atattttttg ttatttaata ataggattat
     2161 tttgtgatat ccaaatataa atcgatatga tctgtgtaag cgacgtaaga gtcaatcaaa
     2221 gaactgatat cttgaaaaaa atatttacaa aagaagaatc ttgtcgaatt gaagattctg
     2281 cagtagatat atcttttttt gagtttaaag aacggggtaa tgattctaat gttagagaac
     2341 tgcaacattc gtttttaatc ccatcattta taaaaacatc agatgatgct gtaaatgaaa
     2401 acagtagttt tagatatcct atggtaaggc ctcctcatca caacgagaat gcatgcatat
     2461 tgttgtttca tggtcttaat gagcgaagct gggataaaca tgcgatatgg gctgaaactt
     2521 tagcaagaat gacaggtaag cccgtaatcc tatttccgat agcattccat attaatagga
     2581 ctccatcgtc atggtataac ccacgtgtgt taatgcattg ggttgcggat aggcgaaaaa
     2641 ggacagcttc tgccgaaaat gttacattcg caaatcttgc tttaagctcg cgcttgagtt
     2701 cctctcctct tcgattttac acttcgggtc gagagacttg ttatgatgta gcacagctaa
     2761 tggacaacat aaaggcgggt aaacatccat atttgaacga aaatactaaa atagatatat
     2821 ttgcatattc tattggagcg ttaatgtcgc aaataatgct tttgtcaaat tacaaaaata
     2881 tgtttgaaaa cgctcgacta tttatgtttt gcggaggttc tatttttaat atgatgaatg
     2941 gaaattcgcc gtatattatg gattctcaag cattccttga tatgattgct tattatcaaa
     3001 atgatcacct atttcaggca ataaataatg acgatccatt tgatttacca tttaaatcga
     3061 tgattgatgc taattattat accgattaca gaaccgattt ttttaataag gcacaaaata
     3121 gaatccaaat tatttctttg gcaaaggata aggttatacc atataaaggt atttgtgctg
     3181 cattaggtgc ggaggcggct gcaaaatgtg taattaaact tgattatcca tttgagtatt
     3241 cgcatgaaat gccatttcct acaaagatga atgctacaaa tgaagttgac aaaatgttta
     3301 tggacgtatt tggaagagcg gctaactttt tatcatagct gcttaccatt gaaagtgagt
     3361 tttatcttca gcaagtttag tgttaggaaa tacagaacga gcctctgcta aaagtttatt
     3421 taaattttgg tatcttgcag aatagtgtcc tattaacagt tgaccaactt ctgctttttt
     3481 tgctatgttg gcagcttgta aagccgttga atgtaatgtg tctttagctc ttgaaatttc
     3541 agattcttca aaagtcgctt catgaaacaa gcaatcaaca ccgtgaatta atggaattat
     3601 gcgttcgcta tatcttgtgt ctgaacaata agcatagctt ttagatttag tagccggtgt
     3661 taatagatct gcattgtgta taatagttcc atcaggcttt atccagtctg ctccttcttt
     3721 aatattcttg atatctttga caccaatttg aaaaaaatca attaaatctt tatttaggtg
     3781 tggttgattc ggtttttctt taaataaaaa tccacatgta ggagttgaat gtactaatgg
     3841 aatagtattt actgttaggc ttctgtcttc gtagattaat tcactctgtt ttggattaaa
     3901 tggctcaaaa tataccttat atcttaaatc gtcgcagaaa taatcaattt gtggttgtaa
     3961 taattcaatt aagtcaggat gagaatgtat atataaatca gcagttctgt gcatcatgct
     4021 cattgtagat ataactgcca ttagaccgaa acagtggtcg ccatgtaaat gagatataaa
     4081 aatatgtccc attctagtta tgtttatgcc taactcacgc attctgaatt gtgtaccgtc
     4141 tgcacagtca atcataaaac ttttgttgtg agttgataaa acttgagaag tagagaatct
     4201 tccaaatgaa ggaattgccg aacccgaccc taatatagtt aattcaaagt tcattttttt
     4261 atcagatata tagttttttt gttttctgtt gcaattgttt tttcatccat caattgtctt
     4321 attactgtaa aaattgtttc ttcagaacaa ttaagttttc ctattaaata agatatttct
     4381 tgtggaccat cctttaaaag atcaattatt tttttgtcaa gattgtttgt ttttttgtta
     4441 ttgctcttct gttttaggca aaaatcgcat ataccacaat tatcagaatc tttttctcca
     4501 aaatatgcta gtagtatgcg actacggcat gcattatctg atgttgaata atcagacatt
     4561 gctttaattc gattttcaaa gcgttctttc cgattttcat atacatcctt tcctatgata
     4621 attctttctg tatgttcacg ttgacgtgta tatactatat agggtgtttt tttgtaagga
     4681 atatagctta taacatttag tcttgacaaa tcaatcaaac ttttaagtac gtctgatttg
     4741 tcaagtttgg tccgttttgc aataagatct tcatcaatgt ttacataatc ggcaaacaga
     4801 cccgtgtaac tccttaataa acatttcatt acaacatctg ttttgtcggg tatgtcgtat
     4861 ttgtacagtt catctcggta catcgaaaac attattcgtg tatgagattc aatctcttct
     4921 gtatactcaa tatatccggc tagttccaat attttcagtg cgctgtgagt aggatttata
     4981 gacaatttaa atttggtaca aaaatcaatc aaattaaaat caaaaacaca tccttctgca
     5041 ctttcaacac ctacctgata gtaataagcc aagtattcgt aggtacgtaa tatttgctct
     5101 ttagtaggaa aattatcaga taaacgtttc tttaattttg taatatcagc attttcatac
     5161 aatagtacag catatgattt taatccgtct cgtcctgcgc gtcctgcctc ttgaaaatag
     5221 gcctctatac tatccggtaa atctatatga ataacagttc ttacatcagc tttgtcaata
     5281 cccataccga aagcattagt tgctactatt acgcggcaat ctcctgactt ccaacgttct
     5341 tgacgattat ctttagctga attaggaagc ccggcatgaa aataatcagc acttatattg
     5401 ttgttattta aaaaatcggc tacttctttt gtcttttttc tgtttctgac atatacgacc
     5461 gaactaccat tgacagaatt aagaatatgt aataactttt gtaacttatc ctctgttttt
     5521 tgtactacgt atgccaggtt ttctctttga aaactttttt tgaaaacatt attttttgcg
     5581 aattgcaatt gtttctgaat gtcctcaaca acctcgggcg tggcagttgc ggttaatgcc
     5641 aacaccggaa catcaggcaa catttttcta atttcggcaa tttttagata tgcaggccta
     5701 aagtcatatc cccattgtga aatgcaatgt gcttcatcta cagcaatcat gctaatatct
     5761 agctgtggca ctttttctaa aaacactcta gtgcctaaac gctccggtga tacatacaga
     5821 aatttgtaat gtccatatat gcagttattg aaggttgtca taatctcttc ataactcata
     5881 cccgaataaa ttgcagctgc ctttatctct ttcttcctta atccatcaac ttgatctttc
     5941 attagtgcaa tcagtggtgt gatgactaag cataaaccat ccatcgataa tgcagggacc
     6001 tgaaatgtta ttgatttacc tccacctgta ggcattaaac ccaatgtatc tttaccactt
     6061 cctacagaac tcacaatatc ttcttgaagg ctacgaaaat catcgtatcc ccaatattgt
     6121 tttagtatag agcgatagtc ggtccggttc attttttata aacacatttc ttccaaacac
     6181 ttatatgcct gacggatacc attaacacca tcaatcgata tcaatcgttt gtctgtttgc
     6241 tctttaaatt ttgtattact atgatgtacc atagcatatt gcaaaatctt attaaatatt
     6301 tcagattggt aataatgtga ctggaaatta ttagtaaagt atgcgcttaa tctattgttt
     6361 tttagtgtta ttttttcaat tcctaaactc atgcacagcc atctaagtcg aataacttgt
     6421 ataagttctt gtgaaacttc aggtatttct ccaaaacgat caaccatttc tttttcaaat
     6481 gatattaatg catttgcctc ttttattgca tctaaacggc gatacaaatc catcctttca
     6541 gatatgtttt gtacataata cgaagggaat aatagctcta agtcggattc gaacacgcaa
     6601 tctgttgcat atatattatc tttttctttg atttcttccg caaatacatc agcaaactcg
     6661 tcgtgtttaa gttcgtgtat tgattcatcc aatattttat ggtatgtttc gtagcctaag
     6721 tctgagataa atccactttg ttctcccccc aaaatattac cggctccacg tatatccaaa
     6781 tcttgcattg cgatgtgtat accgctgcct aaatcggaga agttttctat agcttgcaat
     6841 cttctacgtg aatctgtagg aaggctgctg agtggaggtg ttataaggta gcagaatgcc
     6901 tttttgttgg ttctgcctac acgtcccctc aattgatgca aatcgcttaa tccgaaatgt
     6961 tgtgcgctat taataaacat ggtatttaca tttggtatat ctactcctga ttcgatgatt
     7021 gaagtagcca ataaaatatc atattcgcca tttataaagt ttattaaaat ttcttctaac
     7081 ttttcgctgt ccatttgccc gtgtcctacg caaattcgtg cttttggtac caatttacgt
     7141 aggtgtattt ccaactcagg taaattacta attctgttgt ttacaaagaa tacttgtcca
     7201 cctctgctaa tttcatattc aatagcatcc ttaattacat catcatcaaa tgggattaat
     7261 tccgtttgta taggatatct attaggtggg ggagtgttta tgactgataa atctctagct
     7321 cccattaatg aaaattgtaa tgttcttggt ataggagtag cagacaaagt tagagtatct
     7381 atatttgtac gtaattgtct gagcttttcc tttgccgaaa ctccaaattt ctgttcttca
     7441 tcaattatta gtaagcctaa gtctttgaat ttaacctctt tacctaatat tttgtgtgtg
     7501 ccaatcagaa tatctactcg tcctgctgca gttttttcca atattttttt tgtttctaca
     7561 ggctttcgtg cgcggctaag ataatcgata ttacacggga agcgcttcaa tcttcgcgaa
     7621 aaacttttat aatgctgaaa ggctaagact gtggttggaa caagcacagc tacttgttta
     7681 ttatctgata cagccttaaa tgccgctcta atagctattt cagtttttcc gaagccaacg
     7741 tctccacaaa ttaatcggtc catcggcttc tcgctttcca tgtctttttt tacaacagca
     7801 gtagcttttg attgatcagg agtgtcttcg taaataaatg atgcttctaa ttcttgttgc
     7861 agatagctgt caggagaata agaaaaacct ttttcttctc tacgttctgc gtataatcta
     7921 attaattctc tggcaatgtc cttaactttt ttctttgttt tctcttttaa gttagtccat
     7981 gctgctgttc ctaatttatt taattttgga ggctcgctat ctttactctt atattttgaa
     8041 atacgatgta aggaatggat gtttacaaaa atgatgtcgt cgtctttgaa tgtaagtttt
     8101 atgacttctt gcatttttcc acccatctcc gtacgaatca gtccgccaaa tttgccaact
     8161 ccatgatctg tatgtacaat gtaatctcca atttgaaatt gcattaactc tttcagagat
     8221 agtacaacct tacctgttcg tgtttttgct gatttgagag tgtatttttg gaatcgttca
     8281 aaaatctgat ggtcagtgta aacacatatc tttaggtcgt tatcaataaa tccttcgtgc
     8341 aatatcgata gtaccggtat aaagattata tgttcctctc tatcttcaaa aatatctttt
     8401 aaacgttcaa tttgttttga gttgtcacta agtacatata cttcaaagcc ttcggactgc
     8461 ctttgcttaa aatcggaagc tgttaaatca aaatttttat gaaaaatagg ctgtatactt
     8521 tcattaaatt ggatagtttt tctactgccg aaatgatttc ttagacccca ttcaattatt
     8581 ttatatgttt ttatacgtat ttcaaattcg attgccgtta taaagcattc attatcagta
     8641 ttttcttcaa taatagtttg taatacctca gataagtaat ctgcatcgta taaaccgaat
     8701 aaagtttcta tgggaaagaa atcgaaaata ggaactaaat ttttagtttc tgtatttatt
     8761 gtgtttgata tgattgatac ctcgttttga gaatcttccg acaattgact ttcaatatca
     8821 aaatttctta tcgaatcaac ttcgtctcca aaaaaatcaa ttctgtatgg taaatcattc
     8881 gaatatgaaa acacatctat gatacttcct cgtacagaaa attgccccgg ttcataaaca
     8941 aaatcaattt gctcaaagcc atattcagtc aatgcttcaa taacaaattc gatgcccggt
     9001 ttttctccta cacgtagatg taaagtctct ttttcaagct tattcggctc aattactttt
     9061 tctgataatg cttcgggata agtgactata gtccaatcag acctcatgct aatggcattt
     9121 agagtttctg ttctaagtac ttgattagca gagtctttta gaatgccatc ttttactttt
     9181 ttcttatatg atgatggaaa ataaaatata ttttcatcgc cataaaactg tataagatcg
     9241 ttgtaaaaat acccggcttc ttcgtggtca tgtaatacgt aaacagccgg taaatgatgt
     9301 ccaatttgtg cttcgtgcaa aaaaatggat gcagaagaac cgcaaaaacc atttatttta
     9361 agatttttgg atgaggacga tacccatttt tgaagtgtat caaaatttgg atgttgcctt
     9421 agaattttta aaaaatcgga actgtccatt tactttttgc aaacaatttg catagtctga
     9481 atcgcaaagg taatttgatt ttagtgtttt tgcaaacaaa taaatcttgt acaagttaat
     9541 tttatttcac cctttttaat ccatagattg ttgctgtata aaaatctttt cataattttg
     9601 gaggactaaa aacaaataca atgtcagaca gtattgtcct tataccaact tataacgaaa
     9661 aagaaaatat tgagaatata attcgtgcgg tgttttcgtt gccaaaagaa ttacatattt
     9721 tagttattga tgatggctct cctgatggaa caggagctat tgtgaaaaga atgcaagaag
     9781 agtttcctgg ttccttacat ttaatggaaa gatccggtaa gcaaggatta ggtcgggcat
     9841 atattgcagg atttaaatgg tcgctgagtc atggttatgg atatattttt gagatggatg
     9901 cagatttctc tcataaccct aatgatttat taaaattata tgcggtatgt catgatgaag
     9961 gtgcagatat gtcaataggt tctcgctatg tatcaggcgt aaatgttgta aattggccaa
    10021 tgggaagggt cttgatgtca tattttgcat caaaatatgt tcgttttgta acaggattac
    10081 cggtggcgga tacaacagcc ggttttaaat gctataaacg tgaagtatta gaaacaatag
    10141 aactcgataa aattagattt gtaggctacg catttcaaat agaaatgaaa tttacagcgt
    10201 ataagtgtgg ttttataata aaagaagttc ctataatatt tatcaataga gaacttggaa
    10261 catctaaaat gagtgggggt attttcggtg aagctgtttt tggagtttta aaactcaaat
    10321 ggaatagcct ttttagaaag tatcctaaga aacaagcgta aaagattttt tatgtactta
    10381 ataaaagatg cacatattgt aaatgaaggg cgagttttta aagcatcagt tttaattaaa
    10441 gatgataaaa ttgattcaat atttaaagct gatgttcctg aatctattat acagagtgca
    10501 gaattaattg atgcctctga ttgttggctt atgcctggaa tcatagatga ccatgtacac
    10561 tttcgtgatc ccggtttccc tgaaaaagca gatttttact cagaaacatg tgcagccatt
    10621 gccggtggtg tgacatctgt gatggatatg ccaaatacaa atcctcaaac aacatcaata
    10681 cacaattggg aagacaagat gaatatcgcg tctgataaat cattgtgtaa ttatgccttg
    10741 tatttgggtg ctacgaacga caacctttca gacttgcttc aagcagatcc gactaaagta
    10801 tgtggaataa aattgttttt gggttcatca acaggaaata tgctggttga aaaaaaagac
    10861 gccatagaaa atatatttgc aaaaagcccc ttattgatag ccgttcatgc tgaggatgag
    10921 gaaattatac aaaaaaatag gttgcacttt actaatttat atgctgacaa aattcctata
    10981 gaatttcata catcaattcg ttctgcagaa gcttgcttta aggcatctgc catggcggtt
    11041 gagttggctc ataaatataa ttctcgttta catttggcgc atataagcac ggaaaaagaa
    11101 ttagctcttt tcaaaaataa atcaatagat aaaaagcgca taagttcgga agtttgtgtt
    11161 aattacctat ggtttagtta taaggattat gaaaaatacg gagcaaaaat aaaatgtaat
    11221 ccatcaatta aaactgaaac agataaatgt gctttattga atgcattgtc gtctgacaaa
    11281 atcgatgtta ttgcaacaga ccatgcaccg catttattgt cagaaaaaaa tggatattgt
    11341 atgcaggctg tttcgggtat accaagtgtt cagttctctc tgcctttaat gatggaattt
    11401 gttaaacgag gaaacataag cattgaacaa ttggttacta aaatgtgcca taatccggct
    11461 aaaattttta atattgagaa gcgtggcttt ataagaaagg gttataaggc tgatttaaca
    11521 atagtgcgta gaaaagaact ttggcgtgca gatcaatctt gtgttttgtc gaaatgtgcg
    11581 tggtctccgt acgagggagt agaattcaca acaaaagtgg catatacatt tgtaaacgga
    11641 cgtaaggtat ttgataatgg taaaatgtca aataccttac gtggtgaagc gctctgcttc
    11701 aatgcatgaa aaaaatgttg taaaagtgct attgactgcc tatttaaaaa actgctgttg
    11761 agtctaatgt tgtgctattg tctattgagt cgttcacatc aatagtataa gaattagggt
    11821 atgaagaata ccattgtatt gtcgtggtat ttgcttggtt cctccaattt tcccattctg
    11881 aaccgaattc ttgcaatcta ttgatatttc caatactaat tcctactaga gttattatgc
    11941 tcaatagcca aacaattaat agtgtccatc cgaaccattt ggatcttgat ccgtttttat
    12001 gacgtattat tctaatacat atggttacca atcctattaa cggacataaa attataagta
    12061 aggcgcaaat tccaattata gtagctaaat aagatggtaa taccgtgaaa ggaagtaatc
    12121 ctacaaataa agcgggatta gcaaatacgc atacggctgc tgtaagtaaa gcaccgatta
    12181 caattaatcc gatgaatcct gcaaaacacc ctacaataat tagaatgact ttgcaaattg
    12241 taataaatat atctcccatt gtttttccaa cagatttagc agatgattta aaattatctg
    12301 attctacata atctttggct ttattaaatt ggcctttaat gttttctatc gtaacatctt
    12361 cgcctcgcat ttctagcttt tgagcagctg tacgagcctc aggtgcaata acccacacta
    12421 ctatatataa aagtattgac caaccgaatc caaaaattac agtcaatatt ataagaatac
    12481 gaattaaagt gacatcccat cctaaatata cagcaagtcc tgcggctaca ccgcctaaca
    12541 tgctattttc aggatctcta taaaaatgct tattacgttt ttttgtagca gtattattgt
    12601 ctgtttccgt attggcttct tcgctttcga actgatttgg tctgcctaaa acgtctataa
    12661 cgctatttac gtgatttatt tctacaacat ttttactctc agcatgtaat ttttccgaaa
    12721 ataattcggc aatacgtgct tctatgtctt taataacctc tttgtcctca tctgtattgt
    12781 aatgtgcgct tatttctgaa atatattgtt tcagagtttg ataggcatct tcgtcaatat
    12841 tgaaggcgat accatttaga ttgatagata aagtcttttt cattttatat ttggttttta
    12901 tttgtttgat ttaataaatt caacaacttg tttaatgtct gtccatgcgc catcaaggtc
    12961 ttttaagaat aatttgccgt tttctgttaa ggcgtaatat ttacgtggtg gaccttgagt
    13021 tgattcaacc catttatagt cgagaaggcc actatttttg agccttgtaa gtaatggata
    13081 taatgttcct tcaacgacta tcagtttaac acttctgagt tcttgtatta tatcggaagt
    13141 gtaataaggc tttttgttga ttagcagtag tatgcaatac tccaagaagc cttttcgcat
    13201 ttgtgatgtg atgttgtctg tgctaattgc catatctttc gttttttata ggtgaatatt
    13261 atacaccgat ttttatgaca caaagatatg taatttattt agtactatgc actacaaagt
    13321 actaaataaa ttacaaaatt gacaacattt aacaaattag gcttaaaaaa ctagaattta
    13381 aactagaatt taaactagaa tttaaactag aatttaaact agaatttatg agtccaatct
    13441 ttattttccc agattttgta cgaacgaagt gcttgatata caaacatttc ccatccattg
    13501 tagattgttg ctccttttaa ttctgcttgg tgcagaaata aactgcgtat tggattatat
    13561 atcaaatcgt aaacaagatg atcttttgtg atatattcgt atggtatttc agggcatgct
    13621 tttacattg
//
LOCUS       DAOPID010000011        13376 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_5411_length_13376_cov_3.885519, whole genome shotgun sequence.
ACCESSION   DAOPID010000011 DAOPID010000000
VERSION     DAOPID010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 13376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13376
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5411_length_13376_cov_3.885519"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PK641_00960"
     CDS             <1..180
                     /locus_tag="PK641_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylase"
                     /protein_id="HPG54959.1"
                     /translation="GEKITNYEMEGSVLAGMSALMGHEALTVCLIIANRYGKSFIGDY
                     SQKMKELISLVLDRI"
     gene            177..1121
                     /locus_tag="PK641_00965"
     CDS             177..1121
                     /locus_tag="PK641_00965"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HPG54960.1"
                     /translation="MKLVPDFIDFVKKDFNRKAYTYAAVLLVVLLFLNQHYNWYYTYL
                     NPTYWEGTSMLWYTVFYLSTYFIAAIPFLLIKKDYKVLKNPWFYLKSTFFIMLFGITI
                     GFYSYYDYKFVGLTPNEGAYIKHLLFYLKTTFMPLPFLVLAKLTFDRRLENLYGISAS
                     HQHVSTYLLCLLALVPFVIAASYTSEFQSMYPLFKPWYYHGAFDMSDAELALINSMAY
                     CFHYVGTEWVFRGCLVVGMAAIVGRSAVVPMAVMYCAIHFGKPIGEAISSIFGGYILG
                     ALAYQTRHIWGGIVIHIGIALVMDFAGLFQHYCTSSNR"
     gene            complement(1069..2976)
                     /locus_tag="PK641_00970"
     CDS             complement(1069..2976)
                     /locus_tag="PK641_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG54961.1"
                     /translation="MPKNISDIRNIINGSLDKQLLGRALAQLQLLVNNADSERLEEIK
                     NTYRYLLQYLFDGVEDPDRIDIFRRLLKETYELTDDVCAGYMTTAYSENSCFSKLWNV
                     KRFTDEQIKYCSKLNSDGDKIDTALAVAAITLNCLRIFDEHKVLCLADFCDNKHQTTA
                     LRALTGLVICLTIHQKRLQFYPAINNRLNLLFDNKDTVKQTQVIVKQLIRSKETERIT
                     KDISENIIPKIAKLAPKIREKMDESNFDSEDFEEKNANLQDIIAGSEISEKMRIYSEL
                     QMEGSDINMSTFSQLKAFPFFNQIENWFIPFYKENPAVSQLFQEQADNNKSLVSFLTD
                     DNFLCDSDKYSFCINLSMIPETYRKSMSNQLRMESEQMKEDVIHPDKSASITNRYIQD
                     LYRFFKLFRDKKDYEDIFTYRFDIHKASFFRLINPENEFLPELSDYFFTKEQYENALS
                     AYLKLETFSEPNADLYKKIAFCYQKTLFYTQAISYYEKADIIESDQLGTLRKLAYCHR
                     KSGHYKEALDYYIQIDKLEPNNLNTIFNMGMCSAELRLYDSALNYFYKIDFLNPDKKQ
                     SMNYQLYFGHCLWATGKRKEAINCYREYVPFSKLDEILRLAPVDFTEQDIAFIIDYLR
                     YNNVETDLQNP"
     gene            complement(3002..3943)
                     /locus_tag="PK641_00975"
     CDS             complement(3002..3943)
                     /locus_tag="PK641_00975"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPG54962.1"
                     /translation="MNALTATNFNLKGQTNVYHGKVRDVYSINNDTLIMVATDRISAF
                     DVVLPKGIPFKGQMLNQIAAKFLDETADIVPNWKISTPDPMVTVGYRCEGYPVEMIVR
                     GYLCGSAWRAYKSGVREICGVKIPEGMKENQKFPTPIITPTTKAEIGMHDEDISKEEI
                     IKQNLISAAEYEQLEKYTLALFARGTEIAAKRGLILVDTKYEFGKCNGKIYLMDEIHT
                     PDSSRYFYAEGYQERFEKGEAQKQLSKEFVREWLMDNGFQGKAGQKVPEMTAEIVKSI
                     SDRYLELFEHITGEKFEKAESEDILKRIENNLKKAGF"
     gene            complement(3959..4903)
                     /locus_tag="PK641_00980"
     CDS             complement(3959..4903)
                     /locus_tag="PK641_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPG54963.1"
                     /translation="MTEETYILETADPILFCGVNNANIHLLKVLFPKIRIIARGNAIK
                     AMGDDADVVDFVKKLSLIEQHITKFNTINDAVIIDLVKGKEPIVSTTAENLILFGLNG
                     KAITARTENQQKLVKAFTNHDLMFAVGPAGSGKTYTAIALAVSALKNKQVRKIILSRP
                     AVEAGEKLGFLPGDMKDKIDPYLQPLYDALEDMIPPFKLKEYLETHVIQIAPLAFMRG
                     RTLSDAVIILDEAQNTTSQQIKMFLTRLGFNSKMIVTGDITQIDLPSAKQSGLIDAMH
                     ILKNVKGIAHIEFDKNDIVRHKLVSRIVEAYEKRTEQN"
     gene            complement(4903..5775)
                     /gene="rfbD"
                     /locus_tag="PK641_00985"
     CDS             complement(4903..5775)
                     /gene="rfbD"
                     /locus_tag="PK641_00985"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HPG54964.1"
                     /translation="MKNILVTGAYGQLGNEIRIFSANYPQYGFIFTDVDTLDICDLEA
                     IEKIVSSNNVAYIINCAAYTAVDKAEDNEDLCRKLNADAPENLGKAANKYKAGIIHVS
                     TDYVFDGSSNIPYTEDMKTCPASVYGKTKREGEEKLMAVCQKSFVIRTAWLYSEFGNN
                     FVKTMIKLGKERETLSVVFDQIGTPTYAADLAKAILQIMDYAIKNENDNKYGIYHYTN
                     EGVCSWYDFTLKIHELSNITSCKVSPIESKDYPAKAPRPYYSVLNKSKIKQTFGIAIP
                     HWEDGLKRCLKGLI"
     gene            complement(5778..6332)
                     /gene="rfbC"
                     /locus_tag="PK641_00990"
     CDS             complement(5778..6332)
                     /gene="rfbC"
                     /locus_tag="PK641_00990"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005843153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HPG54965.1"
                     /translation="MEIIETGIQGLLVIKPKIFGDSRGYFCETYNKKAYEAVGIKQNF
                     CQDNLSKSSYGVIRGLHYQLQPESQAKLVSVVIGEVYDVAVDIRKGSPTYGKWYGIRL
                     DAETKTQFFIPQGFAHGFSVLSETAVFTYKCDNFYNPALEGGIQFDDPELGIDWMIPA
                     DKRIISDKDTKHPLLKDIQTNFVF"
     gene            complement(6506..7384)
                     /gene="rfbA"
                     /locus_tag="PK641_00995"
     CDS             complement(6506..7384)
                     /gene="rfbA"
                     /locus_tag="PK641_00995"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HPG54966.1"
                     /translation="MKGIVLAGGSGTRLYPVTKSISKQIIPVYDKPMIYYPISILLLA
                     GIKEILIISTPQDIHLYENLLGDGSTLGVHFEYAIQPSPDGLAQAFIIGEEFIGKDSV
                     CMVLGDNIFYGYNLSNMLQAAASLKDGATVFGYYVNDPERYGVAEFDQDGKVLSLEEK
                     PAKPKSNFAVTGLYFYGNDVVEKAKGLKPSARGELEITDLNRLYLNEGRLTVKLMGRG
                     VAWLDTGTHDSLLEASNFISTIQNRQSLQVACLEEIVYKYGYISRDQLLKLAEPLKKN
                     YYGQYLIKIANEKHIY"
     gene            complement(7452..7853)
                     /locus_tag="PK641_01000"
     CDS             complement(7452..7853)
                     /locus_tag="PK641_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HPG54967.1"
                     /translation="MKEEVRIDKWLWAMRLFKTRSIAADACKKGKIMMSGQNLKPSRT
                     IKVGDIIQIRKSPIIYSFRVLALTENRLGAKLVPEYMENVTTPDQYELLEITKISGFI
                     NRDKGTGRPTKKDRRDLDEFLSDTFFDDEID"
     gene            complement(7859..8422)
                     /gene="pth"
                     /locus_tag="PK641_01005"
     CDS             complement(7859..8422)
                     /gene="pth"
                     /locus_tag="PK641_01005"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPG54968.1"
                     /translation="MKYLIVGLGNIGSEYQETRHNIGFMILDAFAKASNVFFTEKRYG
                     STAECRIKNQTLLLLKPSTFMNLSGTAVRYWLKEENIDLDHLLIVVDDIALPLGTLRL
                     KSKGSDAGHNGLKNIQELISTEAYPRLKFGIGNDFPKGKQIDYVLGKFSSEQQEKLNE
                     RIDLAVEMIRSFCLAGMQTTMNQYNNK"
     gene            complement(8446..9030)
                     /locus_tag="PK641_01010"
     CDS             complement(8446..9030)
                     /locus_tag="PK641_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HPG54969.1"
                     /translation="MRVFELNGTPRTELGKKACKTMRKENLIPCVIYGGKENINFTVT
                     QSAIRKLIYTPEIFEVKLTIGKQTMKAILKEVQYHSVSDNILHVDFIEVDDKKPILFR
                     VPIKTEGLAVGVRAGGKISLEMRKLKIKALCKDIPSSLTINVENLEIGKSIKAGDLSF
                     PNIQIMDNKESLVVCVKSTRAAKDAAATTEAAAK"
     gene            9318..9656
                     /locus_tag="PK641_01015"
     CDS             9318..9656
                     /locus_tag="PK641_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007215820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPG54970.1"
                     /translation="MNKIVGNNLKKMRNASSFSQEEISSALGIKRSAYSNYESGDREM
                     PLKILEKASNLFGCELYDFFEEDAMSDDVILASAFRFDDFCHSDLQEIIKFQDIVKSY
                     IKMERLAVTK"
     gene            9653..9829
                     /locus_tag="PK641_01020"
     CDS             9653..9829
                     /locus_tag="PK641_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG54971.1"
                     /translation="MKANQIKIIEKQVAKFRSDYGLVSADAIYLKSFLIRLNVLTIFR
                     PRSERWSCMSLKEC"
     gene            9899..10360
                     /locus_tag="PK641_01025"
     CDS             9899..10360
                     /locus_tag="PK641_01025"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HPG54972.1"
                     /translation="MYHLFVELNPKPHKCITEDGKKDQAEQNADMFASIFLMPEEGIY
                     KMLSDEEISKHEVKLSSIIRLEHYFSVSRAALLNRLCDLSLIDKSTKDTLSKFYVIKT
                     AEEYGYDTALYKPGNENLCIGDFGEKAHTLFEMEKISEGHYIELLNKLSYK"
     gene            10357..10884
                     /locus_tag="PK641_01030"
     CDS             10357..10884
                     /locus_tag="PK641_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005790552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG54973.1"
                     /translation="MTQKKTRIVLDSDVVIHFIKGDSFSLLLKIFPEYQYTILDVVYN
                     ELSKNNQTRIVIDNVLNYISDRIQMVEFNPDSNLKREYARLLKNLGKGESACMIYCLD
                     NKDVLGSSNLKDIKKYCEDNGITYLTTLDFIYYAYIRNLMSKDDCDEFILKVQEKGSK
                     LPFVDISSYCCTVII"
     gene            complement(10902..11936)
                     /gene="thiL"
                     /locus_tag="PK641_01035"
     CDS             complement(10902..11936)
                     /gene="thiL"
                     /locus_tag="PK641_01035"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HPG54974.1"
                     /translation="MAKTEISTLGEFGLIKHLTQKIELKDAASIKGVGDDAAVMDYSG
                     AKKRVLVTTDLLLEGIHFDLSYVPLKHLGYKAAMVNFSDIYAMNGCPKQITVSIGISK
                     RFSVEDMEDFYDGILLACQRHNVDLVGGDTSASVTGLTISITCIGEAEESKIVYRSGA
                     KNTDLICVSGNLGAAYMGLQLLEREKSLFDKTTEAVQPDFSGKEYILERQLKPEARRD
                     IIEKLSKADILPTSMMDISDGLSSELLHICSESGVGCRVYEDKIPIDYQTAVMAEELN
                     MNLVTAALNGGEDYELLFTVPLALHEDINKIEGIKVIGYISDASEGSVLVTRDGNEIT
                     LKAQGWNPLM"
     gene            11993..12694
                     /locus_tag="PK641_01040"
     CDS             11993..12694
                     /locus_tag="PK641_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPG54975.1"
                     /translation="MSLSVAYLNKRYGTQQVLKDLSFSIGDGEIVGFLGPNGAGKSTT
                     MKILTGCLQPDGGRITLEDIDVLLYPNKAQALMGYLPENNPLYVDMYVKEYLYFVAGL
                     YKMNSKLASERVDEMIERTGLTNEYKKRICHLSKGYRQRVGLAQAMIHDPKVLVLDEP
                     TTGLDPNQVIEIRELIKDFGKDRTVLFSTHIMQEVTAVCNRFLVLRKGELVADMKTSE
                     LEGRDLEKLFFSLTN"
     gene            12697..>13376
                     /gene="ruvB"
                     /locus_tag="PK641_01045"
     CDS             12697..>13376
                     /gene="ruvB"
                     /locus_tag="PK641_01045"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HPG54976.1"
                     /translation="MDEDFDIRKVQTDENGGDYENILRPMAFTDFKGQNKVVENLKIF
                     VQAARLRGEPLDHVLLHGPPGLGKTTLSNIISNELGVGFKLTSGPVLDKPGDLAGLLT
                     NLEPNDVLFIDEIHRLSPVVEEYLYSAMEDYRIDIMIDKGPSARSVQINLNPFTLIGA
                     TTRSGLLTSPLRARFGINCHLEYYDAEVLTGIVKRSSNILDTECYEDAAVEIARRSRG
                     TPRIANAL"
BASE COUNT         3917 a         2246 c         2420 g         4793 t
ORIGIN      
        1 ggcgaaaaaa ttactaatta cgaaatggaa ggctctgtac ttgccggcat gtcagcatta
       61 atggggcatg aggcactaac tgtttgcttg attattgcta atagatacgg caaaagtttt
      121 attggagatt attcacagaa aatgaaagaa ttaattagct tggttttaga tagaatatga
      181 aacttgtacc tgattttatt gatttcgtta aaaaagattt taacaggaaa gcctatactt
      241 atgctgcagt attacttgta gtattgctgt ttttgaatca gcactataat tggtattata
      301 cctatttaaa tcccacatat tgggaaggta catcaatgct ttggtataca gtgttttatc
      361 tatcaacata ttttattgcc gctattccgt ttttacttat aaagaaagat tacaaggttc
      421 taaaaaatcc ctggttttat ttgaaatcga cattttttat aatgcttttt ggtataacaa
      481 tcggcttcta ttcttattac gattataaat ttgtaggttt aactccgaat gaaggtgctt
      541 acattaaaca tttgctgttt tatcttaaaa ctacatttat gccactgcct tttcttgttt
      601 tggctaaact gacctttgac aggcgtttgg aaaatttata cggcatatcg gcatcacatc
      661 aacatgtttc tacatattta ttgtgtttat tggctttagt accatttgtt attgctgcat
      721 catatacctc cgaattccaa tctatgtatc cattatttaa gccttggtat tatcatggtg
      781 cttttgatat gagtgatgct gaattggcgc ttattaacag catggcatat tgtttccatt
      841 atgtaggtac tgaatgggtt tttagaggat gtttggttgt tggaatggct gctattgttg
      901 gacgcagcgc agttgtacct atggcagtaa tgtattgcgc tatacatttc ggcaagccta
      961 ttggtgaggc tatatcatct atattcgggg gatatatttt aggagcctta gcttatcaga
     1021 cacgtcacat ttggggtgga atagttatcc atataggcat cgccttagtt atggattttg
     1081 caggtctgtt tcaacattat tgtacctcaa gtaatcgata ataaaggcaa tatcttgctc
     1141 cgtaaaatcg accggagcta atcgtaatat ttcgtctaat tttgaaaatg gcacatactc
     1201 acgatagcaa ttgattgctt ctttacgttt gcccgttgcc cacaaacaat gaccaaaata
     1261 cagttgataa ttcattgatt gcttcttgtc aggatttaag aaatctattt tataaaagta
     1321 gtttaatgca ctatcgtata gtcgtaattc tgcactacac atacccatat taaaaatagt
     1381 gttcaaatta ttaggctcta gtttgtctat ttgaatgtaa tagtctaagg cttctttgta
     1441 atgtcctgat tttctgtggc aatatgccag tttacgcaag gttcctaatt ggtctgattc
     1501 gattatatcg gctttttcgt agtacgaaat agcttgagta tagaataatg ttttttgata
     1561 acagaatgct atttttttat ataaatcggc attaggctct gagaatgttt ctaatttcag
     1621 atacgctgat aaggcatttt cgtactgctc ttttgtaaaa aagtaatctg acaattctgg
     1681 caaaaattca ttttcaggat ttattagtct gaaaaatgat gctttgtgta tgtcgaatct
     1741 gtaagtaaag atatcttcat aatccttttt atctctgaat aatttaaaga aacgatatag
     1801 gtcctgaata taacgattag tgatggatgc tgacttgtct ggatgtatta catcttcctt
     1861 catctgttcg ctttccatac gtagctgatt actcattgat tttctgtatg tttcaggaat
     1921 catactcaag tttatgcaaa acgagtattt gtctgaatcg cataaaaaat tgtcgtcagt
     1981 tagaaatgaa actagagatt tattgttatc ggcttgctct tgaaatagtt gacttacagc
     2041 aggattctct ttgtagaatg gaataaacca attttctatt tgattgaaaa acgggaaagc
     2101 ttttagttgc gaaaaagtgc tcatatttat atcagaacct tccatttgta attctgaata
     2161 aattctcatc ttttctgaaa tttcactacc tgctattata tcttgcagat tggcattttt
     2221 ctcttcaaaa tcttcagagt cgaagttgct ttcatccatc ttctcgcgaa ttttaggcgc
     2281 aagtttcgct attttgggga tgatattttc gctaatatct ttggtgatac gttcggtttc
     2341 cttactccta atgagttgtt ttacaattac ttgtgtctgc ttaacggtat ctttgttgtc
     2401 gaataacaag ttgagtctgt tatttatagc aggatagaat tgtagccttt tttgatgtat
     2461 ggttaggcat attacaagcc ctgttaatgc gcgtaaagct gttgtttgat gtttgttgtc
     2521 gcaaaaatct gctaaacaaa gcactttatg ctcgtcaaat atgcgtaagc aattaagggt
     2581 tattgcagct actgctaatg ctgtatcgat tttgtctccg tcagaattta gtttgctgca
     2641 atatttaatt tgttcgtcag taaaacgttt aacattccac aatttcgaaa agcatgaatt
     2701 ttcgctgtat gccgttgtca tatatcctgc acatacatca tctgtaagtt cgtaagtttc
     2761 ttttaataga cggcgaaaaa tatcgatacg atcaggatct tcaacaccat caaacaagta
     2821 ttgtaataaa taccgatagg tgtttttgat ctcttccaaa cgctcgctat cggcgttatt
     2881 tacaagtagt tgtaattgtg ccaatgctcg cccgagtaat tgtttgtcta atgaaccatt
     2941 gatgatattt cgtatgtcag atatgttctt aggcataatt tatgcttttg tgtaggaatg
     3001 attaaaaacc tgcttttttt aagttgtttt ctatacgttt aagaatgtct tctgattctg
     3061 ctttctcaaa tttctctcca gtaatatgct caaatagttc aaggtatctg tcgctgatgc
     3121 tttttactat ttcagctgtc atttcaggaa ctttttgtcc tgcttttcct tgaaatccat
     3181 tgtccataag ccattcgcga acaaattctt tagataattg tttttgtgct tcaccttttt
     3241 caaaacgttc ttggtatcct tctgcataaa aatagcgtga tgaatcaggt gtatgaattt
     3301 cgtccatcaa atagattttg ccattgcatt taccgaattc gtattttgta tcaaccaaaa
     3361 tcaaaccgcg ttttgctgca atttctgtgc ctcttgcaaa tagagctaag gtgtattttt
     3421 cgagttgctc atattctgca gccgaaatca aattttgctt gataatttct tcttttgaaa
     3481 tatcttcgtc gtgcattccg atttctgctt tggttgtagg agtaataatt ggtgtaggaa
     3541 atttttgatt ttctttcatg ccctcaggta tcttaactcc acatatttcg cgaactccac
     3601 ttttataagc acgccatgcg ctaccacata agtatccgcg tacaatcatt tctacaggat
     3661 agccttcgca acgatatccg acagtaacca tagggtcggg agttgaaatt ttccaattag
     3721 gtacaatgtc tgcagtttcg tctaagaatt ttgctgcaat ttgattcaac atttgtcctt
     3781 taaaaggaat tcccttgggt agcacaacat caaacgcaga tatacggtct gttgctacca
     3841 ttataagtgt atcattgttg atactgtata catcacgtac tttgccgtga taaacatttg
     3901 tttgtccttt tagattaaaa ttggttgcag ttagtgcatt cataatgttt atttattatt
     3961 agttttgttc tgttcgtttt tcgtaggctt caactattcg acttactaat ttgtgtctta
     4021 ctatatcatt cttatcaaat tcgatatgtg caatgccttt tacattttta agaatatgca
     4081 ttgcatctat aagtccggat tgctttgcac taggtaagtc tatttgtgtg atatctccgg
     4141 ttacaatcat tttagaatta aaaccaagcc ttgttaagaa catcttaatt tgttgagatg
     4201 tagtattttg tgcttcgtct aatatgatta cggcatcact caatgttctt cctctcataa
     4261 aagccaatgg agcaatttga ataacatgtg tttctaagta ttcttttagc ttaaaaggag
     4321 gaatcatatc ttctaatgca tcgtataatg gctgtaagta ggggtcaatc ttgtctttca
     4381 tatcacccgg caagaaacct aacttttcgc ctgcttcaac agccggacgg ctcagtatta
     4441 ttttgcgtac ttgcttattc tttaaagcgc ttactgccaa tgcaatagca gtgtatgtct
     4501 tgcccgatcc tgcaggacct actgcaaaca ttaaatcgtg atttgtaaag gctttaacga
     4561 gtttttgttg attttcggta cgtgcagtaa tagcttttcc gtttaaacca aacaaaatca
     4621 gattttcggc agtggtactt acaatcggct ctttgccttt tactaggtca attataacag
     4681 catcattaat ggtgttgaat ttggttatat gttgctctat aaggctgagt ttctttacaa
     4741 agtctacaac atctgcatca tcacccatag cttttattgc attaccgcgt gcaataattc
     4801 tgatttttgg aaacaatact ttcagcaaat gaatatttgc attattaact ccgcaaaaca
     4861 gtataggatc tgctgtttct aatatgtatg tttcttctgt cactaaatta atcctttaag
     4921 gcaacgttta aggccatctt cccaatgtgg aatagctatg ccgaatgttt gttttatttt
     4981 gctcttattc aacacactgt aatacggacg tggtgctttt gcggggtagt ctttactttc
     5041 gataggagat actttgcagc ttgtaatatt gctaagttcg tgaatcttta aagtaaaatc
     5101 gtaccaactg caaacgcctt cgtttgtata atgatagatg ccgtatttat tgtcgttttc
     5161 atttttaatg gcataatcca ttatttgtaa tatggctttg gcaagatcag ctgcgtaggt
     5221 tggagttcct atttggtcga acacaaccga taatgtttcg cgttctttgc caagtttaat
     5281 catagtcttt acaaaattgt ttccgaattc ggaatacaac catgctgttc tgataacgaa
     5341 ggatttttgg catactgcca ttaatttttc ttctccttcg cgttttgttt tgccgtaaac
     5401 agatgcagga catgttttca tgtcttctgt gtatggaata tttgaagaac catcaaaaac
     5461 atagtcggta gaaacgtgaa taatgcctgc tttgtattta ttagctgctt tacctaagtt
     5521 ttcgggagca tcggcattaa gttttctgca taagtcttca ttgtcttctg ctttatctac
     5581 agcggtgtat gcagcacaat ttatgatata cgctacatta ttgcttgaaa ctatcttctc
     5641 aattgcctct aaatcacata tatccaatgt atcaacatct gtaaaaataa atccgtattg
     5701 tggataattg gcagagaata tgcgtatttc gttacctaat tgcccgtatg ctcctgttac
     5761 taatatgttt ttcataatta aaatacgaag tttgtttgta tatcctttag caatggatgt
     5821 tttgtgtctt tatcggaaat aatgcgtttg tcggcaggta tcatccaatc gattcctaat
     5881 tcgggatcat caaactgaat tccaccttct aatgccggat tataaaaatt atcgcactta
     5941 tatgtaaaaa ctgctgtttc acttaaaacg gaaaatccgt gagcaaatcc ttgaggaata
     6001 aaaaattgtg tttttgtttc tgcatcaagt ctaattccat accattttcc ataagtaggg
     6061 gagcccttcc tgatatcgac cgctacatca tatacttcac caataactac tgatactaac
     6121 tttgcttgac tttcaggttg taattgataa tgtaatcctc taattacacc ataacttgat
     6181 ttggatagat tgtcttgaca aaagttttgt ttgataccta ctgcttcgta tgctttttta
     6241 ttataagttt cgcaaaaata tccacgtgaa tcaccaaaaa ttttaggctt tatcactaat
     6301 aatccctgaa tgcctgtttc gattatttcc attgtttgtt gttaaattgt ttaatcgttt
     6361 aatcgtttaa tgtgtatggg cgaattgcat tcgcccgttg gattcgcatt gtgatgatgt
     6421 ttcgttgttc gattttgtag gggcattatt gatcatttca acattggatt atacacacta
     6481 cacattacac attacacgtc tttcatcaat aaatatgttt ctcgttcgca atttttatca
     6541 aatattgacc gtaataattc tttttaagcg gttcggctaa tttaagaagt tgatcgcggc
     6601 ttatgtatcc gtatttatat acaatttctt cgagacatgc tacttgcaag ctttgacgat
     6661 tttgtattgt agatataaaa ttagatgctt cgagcaaact gtcgtgtgtg ccggtatcaa
     6721 gccatgcaac gcctcttccc attagtttga cagttaatct gccttcgttt aaataaagac
     6781 ggttaaggtc agtaatttct aattcgccac gtgctgatgg tttcaagcct ttggcttttt
     6841 ctacaacatc gtttccgtaa aaatataaac ctgtaactgc aaaattagat tttggcttag
     6901 ccggtttttc ttctagtgat aaaaccttac cgtcttggtc aaattctgct actccatagc
     6961 gttcagggtc atttacgtaa tatccgaata ctgtggctcc atcttttaga gaagcagccg
     7021 cttgcagcat gtttgataaa ttgtatccat agaaaatatt gtcgccaagc accatgcaaa
     7081 cactgtcttt accaataaat tcttcgccaa taataaatgc ttgtgccaaa ccatcgggtg
     7141 atggttgtat cgcatattca aaatgtacgc ctaaagtaga tccgtcacct aaaagatttt
     7201 catacagatg tatgtcttga ggagttgaaa tgattaaaat ttccttgatt cctgctaaca
     7261 gaagtattga tatgggataa taaatcatcg gcttgtcata aaccggaata atctgtttag
     7321 aaatgctttt tgttaccgga tataagcgcg tgcctgatcc gcctgcgagt acaattcctt
     7381 tcattatgta ttgttttatt agtgtaagct aaacattgaa acaatcttac aaaagtaata
     7441 ataaaattta attaatcaat ttcatcatca aagaaggtat ctgataaaaa ttcgtctaaa
     7501 tcgcgtctgt cttttttcgt tggacgtccc gtacctttgt cgcggttaat gaagccactt
     7561 attttggtta tttccagtag ttcgtattgg tcgggagtag ttacgttttc catatattcg
     7621 ggcactaatt tagcccctaa acggttttct gttagtgcta aaacgcgaaa tgaatatatt
     7681 ataggtgatt ttcttatctg aatgatatct ccgactttga tagtgcgtga gggtttgaga
     7741 ttttgtccgg acatcattat tttgcctttt ttacaggcat cagcagcaat gcttctggtc
     7801 ttaaataaac gcattgccca caaccattta tcaatccgta cttcttcttt catcgatatt
     7861 atttgttgtt gtattggttc atagtagttt gcatgccggc taagcagaaa ctacgaatca
     7921 tttcaacggc aaggtcgatt cgttcgttaa gtttttcttg ttgttcgctt gaaaatttac
     7981 caagtacata gtcgatttgt ttcccttttg ggaaatcgtt accaatgccg aattttaagc
     8041 gaggataagc ttcggtagat attaattctt gaatgttttt taatccgtta tgtccggcat
     8101 cacttccctt agattttaac cttaaagttc ctaagggtaa ggcaatatca tctacaacaa
     8161 taagcaaatg gtctagatcg atattttctt cttttagcca ataacgaaca gctgtcccac
     8221 tcaaattcat gaatgtagat ggctttagca gaaggagagt ttgattctta attctgcatt
     8281 cggcagtaga accatagcgc ttctctgtaa aaaaaacatt ggatgccttt gcaaaggcat
     8341 ccaatatcat aaatcctatg ttgtggcggg tctcttgata ttcagaacca atattaccca
     8401 atccaacaat taagtacttc actttgcttg atgtgcttat tattattatt tagcagcagc
     8461 ttctgttgta gcagcagcat ccttagctgc acgtgttgat ttaacacata cgaccaggct
     8521 ttctttatta tccatgattt gtatgttagg gaatgacaga tcacctgctt ttatagattt
     8581 gcctatttcc aagttctcaa catttattgt taatgaagaa ggaatatctt tgcataatgc
     8641 tttgattttc aatttacgca tttccaatga tattttacca ccggcgcgta cacctacagc
     8701 taatccttca gttttgatag gaacacggaa taagataggt tttttgtcgt ctacttctat
     8761 aaagtcaacg tgtaaaatat tgtcagatac agaatgatat tgaacttctt tcaatatagc
     8821 tttcatagtc tgtttgccga tagttaattt aacttcgaaa atttcaggag tgtaaattaa
     8881 tttacgaata gcagattgtg ttactgtaaa attgatgttt tcttttccac cataaataac
     8941 gcaagggatt aaattttctt tgcgcattgt tttgcaagct tttttgccta gctcggttct
     9001 tggagttccg tttaattcaa atactctcat gataaataat tttgtgttac taaaatatgg
     9061 ccatacgtac attaagtgat taagtggcct aattcccatc acacttgtct ttttgacaaa
     9121 gcgccgcaaa gttagtaaaa aaacttgaat aaatacgata tgatgcgaaa gtttttttta
     9181 gtttgccgaa tgatttgctc agagatggag aatactaagg taatactatt gtaaatatgt
     9241 taaatatgtg gcaaaatgta ttgtaattaa attatttttg taattttgcc acataatact
     9301 tgtaaatata ataggtcatg aataaaatag taggaaataa tttaaagaaa atgagaaatg
     9361 cttcttcgtt ctcacaagaa gaaatttctt ctgctttagg tattaaacgt tctgcttata
     9421 gtaattatga gagtggtgat agagaaatgc ctctgaaaat attagaaaag gcatcaaatt
     9481 tatttggatg cgaattgtat gacttttttg aggaagatgc aatgtctgat gatgtaattt
     9541 tggcttctgc ttttagattt gatgacttct gccatagtga tttacaggaa attattaagt
     9601 ttcaggatat tgtaaaatct tatataaaaa tggagcgttt agctgtgaca aaatgaaggc
     9661 aaatcagata aaaataatag aaaaacaagt tgctaagttc cgttcagatt atggattagt
     9721 gagtgctgat gcaatttatt taaagagttt tttaattaga ttaaatgtat taacgatatt
     9781 cagacctcgt tctgaaagat ggtcatgtat gagtttaaaa gagtgttgat cgcattgttt
     9841 taggttaatc aattcaaatc acccaaaggg tagacaacat tttacaattg cgcatgagtt
     9901 gtatcactta tttgtagagc taaatcctaa acctcataaa tgtattacag aagatggaaa
     9961 aaaagatcaa gcagaacaaa atgctgatat gtttgcttct atatttttga tgccggagga
    10021 aggtatttat aagatgttat ctgatgaaga aatatcaaaa catgaggtta aattgtcttc
    10081 aattattagg ttagaacatt atttttctgt gtcgagagct gctttactaa atcgtctttg
    10141 tgacttgtct cttattgata aatcgacaaa ggatacttta agtaaatttt atgtaataaa
    10201 gactgccgaa gaatatggtt atgatactgc attatataag ccgggtaacg aaaatctatg
    10261 cataggtgat ttcggtgaaa aagcacatac tctttttgaa atggaaaaaa tttctgaggg
    10321 tcattatatt gagttgttga ataaattgag ttacaaatga cgcaaaaaaa aactcgtata
    10381 gtgttagact cagatgtcgt aattcacttt attaagggag atagtttctc tttgctttta
    10441 aagatatttc ctgagtatca atatacaatt cttgatgttg tatataatga attatcaaag
    10501 aataatcaga ctcgtatagt tattgataat gtgctgaatt atatatcaga cagaatacag
    10561 atggtagaat ttaatcctga ttctaattta aaacgagaat atgccaggct actcaaaaat
    10621 cttggtaaag gagagagtgc gtgcatgata tattgtttgg ataataaaga tgtgttaggc
    10681 agtagtaatt taaaagatat aaaaaaatat tgcgaagata atggcattac atatctcact
    10741 actttagatt ttatatatta tgcttatatt agaaatttaa tgtcaaaaga tgattgcgat
    10801 gaatttattt taaaagtaca ggaaaaaggt agcaaacttc catttgtgga tatttcaagc
    10861 tattgttgta ctgtaattat ttgatttgtt ttgttgttga attacatcaa cggattccag
    10921 ccttgtgctt taagggtgat ttcgttgccg tctcgcgtaa ctaaaacaga accttctgaa
    10981 gcatcagaaa tgtatcctat aactttgatt ccttcaattt tattaatatc ttcgtgaagt
    11041 gctaaaggaa ctgtaaacaa taattcataa tcttcaccgc catttaaggc tgcagtaaca
    11101 agattcatgt tcaattcttc agccatgaca gcagtttggt aatctatagg tattttatct
    11161 tcataaacac ggcatcctac gccactttca gaacatatat gcagtaattc tgatgataat
    11221 ccgtcagaga tatccatcat tgatgttggt agtatatcgg ccttacttaa tttttcaatt
    11281 atatcgcgtc tggcttcggg cttcaattga cgttctagaa tatattcttt gcctgaaaaa
    11341 tcaggttgaa cagcttctgt tgttttatca aataacgatt tttcgcgttc taatagttgt
    11401 agtcccatgt aagcagcacc aagattacct gacacacaaa ttaaatcggt gttttttgcg
    11461 ccgctgcgat atacaatttt cgattcttca gcttcgccta tacatgtaat acttattgtg
    11521 agtcctgtta cggatgcaga tgtatcacct ccgactaaat cgacattgtg acgttgacat
    11581 gctaggagga taccatcgta aaaatcttcc atatcttcga ccgagaagcg ttttgaaata
    11641 cctatggata ctgtaatctg ttttgggcat ccgttcattg cataaatatc agaaaaattg
    11701 accatagcag ccttataacc caaatgtttt agcggaacat agcttaagtc aaaatgtatg
    11761 ccttcaagaa gtaggtcggt agttactaaa acgcgttttt ttgcgccgct gtagtccatt
    11821 actgcagcat catctccaac acctttaatg ctggcagcat cttttaattc gattttttgt
    11881 gttaaatgtt ttattaaacc gaattcgcct aatgtagata tttctgtttt tgccataatt
    11941 taagtatttg gggagacaaa ggtacaaaat attatttacc tttgcatctt atatgagttt
    12001 atctgttgca tatctaaata agcgctacgg tacccaacaa gtactgaaag acctttcttt
    12061 ttctattggc gatggagaaa tagttggctt tttgggccct aacggagccg gaaaatctac
    12121 aactatgaaa atcttaacgg gatgcctaca acccgatggt ggtcgcatca ccctagagga
    12181 tattgatgtt ttgctgtatc caaataaagc tcaagctctt atgggatact tacccgagaa
    12241 taatccctta tatgttgaca tgtatgtaaa ggaatatttg tattttgtgg caggactata
    12301 taagatgaat tcgaaacttg catccgaacg tgttgatgag atgatagaaa gaacgggatt
    12361 gactaacgaa tataaaaagc gtatctgcca tctttctaag ggatatagac aacgtgtggg
    12421 tttagcgcag gcaatgattc atgatccgaa agtgcttgta cttgatgagc ctacaaccgg
    12481 tttggatccg aatcaggtaa tagaaataag agaacttata aaagatttcg gtaaagatcg
    12541 cacagtactt tttagcacgc atattatgca agaagttact gctgtttgta atagattctt
    12601 ggttttaaga aagggtgaac tggttgctga tatgaaaacc tcagaacttg aaggtcgtga
    12661 tcttgaaaaa ttattttttt ctttaactaa ctagctatgg atgaagattt cgacataaga
    12721 aaggttcaga ctgacgaaaa cggcggagat tatgaaaaca tcttacgtcc aatggccttt
    12781 acagatttta agggtcagaa taaagtagtc gagaatctta aaatttttgt tcaagcagca
    12841 aggttgcgtg gcgaaccgct tgatcatgtg cttttacatg gacctccggg tttaggtaaa
    12901 actacattgt caaacattat atctaacgaa ttaggggtag gctttaaatt aacttcaggg
    12961 cctgttttag acaaacccgg agatttagcc ggattgctaa ctaacctaga acctaatgat
    13021 gtgcttttta ttgatgaaat tcatcgttta agtccggttg ttgaagagta tttatattca
    13081 gcaatggaag attatcgaat agatataatg attgataaag gaccgagtgc gcgttctgta
    13141 caaatcaatc ttaatccttt tactttaata ggagcaacaa cacgtagtgg attgcttaca
    13201 agtccgctaa gagctcgttt tggtattaat tgccatctcg aatattatga tgctgaagtt
    13261 ttaacaggca tcgtaaaaag gtcttcaaat attttagata cagaatgtta tgaggatgct
    13321 gccgttgaga tagctcgtag aagtagggga accccacgta ttgcgaatgc cttatt
//
LOCUS       DAOPID010000012        12937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_5679_length_12937_cov_4.741500, whole genome shotgun sequence.
ACCESSION   DAOPID010000012 DAOPID010000000
VERSION     DAOPID010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 12937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12937
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5679_length_12937_cov_4.741500"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..1582)
                     /locus_tag="PK641_01050"
     CDS             complement(8..1582)
                     /locus_tag="PK641_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HPG54977.1"
                     /translation="MDSKLILDVQPEQISIALLEDSRLVELNKESRSATFSVGNVYLA
                     KVKKLMPGLNAAFVDVGYEKEGFLHYLDLGTYFTTITEFTNGMTSNKKKLPSMNKVQF
                     RPELARTGLITDILTQGKEILVQVTKEPISTKGPRLTSEISIAGRSLVLLPFSNKISI
                     SQKITSNEERNRLKKILQAVKPKNYGLIIRTVAEGREQSELENELLVLIKRWEDTLIK
                     IQKTKSTPSLVVEEIGRAESILRDHFNPDFESIYINDRTIYEEIKAYISLIEPQKANI
                     VKFYDDELPIFDIFNITKQIKSSFGRIVPFRKGAYLVVEHTEALHVIDVNSGTRAKVG
                     NLQEENAFEVNMLAVDEVARQLRLRDMGGIVVIDLIDMNSAEHKQKIYDKMRENMQSD
                     PAKHNVLPLTKFGLMQITRQRVRPNMLINTQETCPTCYGNGTIRPSLLFADALVGKID
                     ILYHNLKLKKFTLFVHPYVYAYIKHGFPSMALKWKFKYGFGIKVRPNQDFSFLQYKFI
                     DKDKNELDLTDLMEMA"
     gene            complement(1849..2124)
                     /locus_tag="PK641_01055"
     CDS             complement(1849..2124)
                     /locus_tag="PK641_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004353167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPG54978.1"
                     /translation="MTKAEIVNEISNTTGIDKVAVMQVVESFMEEVKKSLASKQNVYL
                     RGFGSFVVKQRAEKVARNISKNVTITIPAHVIPSFKPAKTFIESVKK"
     gene            2569..3282
                     /locus_tag="PK641_01060"
     CDS             2569..3282
                     /locus_tag="PK641_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG54979.1"
                     /translation="MKRITFIFICVILSCSVSFGQLKKSRIGSNSKQYVSLSGGANKP
                     LMDIHTDFEPSCSFSYTYYFLPKLGLRGALEYNWFTGQSPVGHPNYYAHIVELSAQVV
                     FNYYVYTGSRRENNSVSFIPERAYIFGGIGALCYLREQVNYAPAFTASFPIGTGLQFP
                     IRDNWAWGIDFTWHFTIADDIDNYPVQKSFTDGYPTLMLTFTYKIPDQKRSGTGYGYK
                     SSKKGLKCDPRKGCEFTFD"
     gene            complement(3284..4318)
                     /gene="mltG"
                     /locus_tag="PK641_01065"
     CDS             complement(3284..4318)
                     /gene="mltG"
                     /locus_tag="PK641_01065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPG54980.1"
                     /translation="MNKKNIYLIIVLLVCVSILEISAGAYYFLQYPNIQTSQKKYIDF
                     YVPKNSTWQSVKDSLKSNNLIKSEKSFDFALKLIGKKKQAMCGKYVLEPNISNRAFIN
                     RLAYGMSHHVNIELKMTRYTEWVARNLSRQMEVDSASLANYLLSDSLLLRYNLNKDNS
                     LALFIRFSKEMSWCASPAEIVAEITKEYDKFWNPERRALASKTGLTIPQIHILASIIE
                     EETNEYEDKCMVAGVYINRIHKGMPLQSCPTVRFAWGDFTIDRILKTHLEIESPYNTY
                     KNAGLPPGPIRIPSEKSIDAVLNYVHHRYLYICAKSDFSGTHEYAVTFQEHSRNARKY
                     QRELNKRGIK"
     gene            4382..4468
                     /locus_tag="PK641_01070"
     tRNA            4382..4468
                     /locus_tag="PK641_01070"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4416..4418,aa:Ser,seq:gct)
     gene            complement(4486..5868)
                     /locus_tag="PK641_01075"
     CDS             complement(4486..5868)
                     /locus_tag="PK641_01075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPG54981.1"
                     /translation="MKARFFSLLDNLNIEGVDKTYVLPSPPTNHDAIDYIRTHTPDGY
                     AIVYTKKTALDLGQNAIKRMIDVASSTGAGMLYSDYYHVKGDQKEAVPTIDYQLGSVR
                     DDFDFGPVILFSADALKTSIATDYLYAALYDLRLQTAKKFPIIHLNEFLYTEFEEDKI
                     SSEEKQFNYVDPKNRAVQIEMESACTNYLKSIGAYLTADFKKISFDDAQFENQASVII
                     PVKNRAKTIADAIESVLSQATNFPFNLLIIDNHSTDGTSEIIERFAKSDNRVVHIVPE
                     RLDLGIGGCWNCGINNAKCGKFAIQLDSDDVYQDQHTLQTIIDGFYAQNCAMLIGSYT
                     ITGFDMKILPPGLIDHKEWTNENGHNNALRINGLGAPRAFYTPLLRSIQLPNTSYGED
                     YAMGLRISREYKIGRIYNSLYLCRRWEGNSDASLNIDKLNKNNLYKDRLRTIEIMARI
                     QFNKTKIPSE"
     gene            complement(5875..8532)
                     /locus_tag="PK641_01080"
     CDS             complement(5875..8532)
                     /locus_tag="PK641_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG54982.1"
                     /translation="MKPARLSILVLFFMAAEILLAAKPSLPLITKFRYLPNIVTNKKE
                     YSVTKTVVSKDKLTVYLEDEFSNLIFREEMLPDVYDSLRVQLPSEYASYELSIISRNK
                     YIEEFVPNFLRKNLPIDKSRMPNYKSGGSVVKKESQLLFPTQGLTDRNIALWGSHGLY
                     FNRTQNRWEWQRPRLFGTVEDLLTASIVIPFLVPMLENSGAQVFMPRERDIQMEELYL
                     CKTTISSDSSEFTGTVNRAGTYRIKIWYGKETKASGQILCKIEHAGIVNNYKINANMG
                     AGTWIDIDGLYFNNDLRVSFVMADKKDALCVDSVKIGGGISHFGNGYPRFAEAARYNL
                     QQAGLPDSILYDQKPGYKSDYYDDIYSRSKWVNFLAGGSHVYPSYPGLKIPIDAVLAV
                     HTDAFTMGADSVVGTLLVCTSDSVYPSGYSKMAGHDLADRIRTQIIDDVSKLKCKNWS
                     SRGIWHQKYVETLVPAVPSVIIELFSHLNYQDMLYGLQPQFKFLLARSVYKGVLKFLA
                     EHNGQSYVVQPLPVRSFGAELANDSVSLSWKPVSDSLEETAYPVRYILYTRKNDGSFD
                     NGQIVKSNSISLAVKPDTIYSFKVTASNEGGESFPSEILSVCNKSESKGTVMIINGFD
                     RIGGPESFDCGSQGGFLYDRDFGVPYMYDIAYTGNQIEFDKSIEYQSNDYPGFGASRS
                     NYETQIIAGNTFDYAYVHGKAIYDNGYSFVSSGKDAVMSGDINLSSYDVLDIIMGKQR
                     TVDGYELYPHKLQSAITRYIKKANKKVIITGAYIGTESCLFTEKALRYKLRAPWASTN
                     GVIITPNNESIYTLQMQANSSQYFLQNVDALQQMDKIAEVLLSYEENKVSAALGFNRN
                     YKVVAAGFPIEALTSDSQRTEVMRLFLNY"
     gene            8636..9469
                     /locus_tag="PK641_01085"
     CDS             8636..9469
                     /locus_tag="PK641_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HPG54983.1"
                     /translation="MKLIADSGSTKTSWVLTKKSNIIQQITTDGINPYYQDENKICET
                     IKEQLFTKIESINIDEIYFYGAGCASPEKNKMIADALGQFFPTSIIAVNSDLLGAARS
                     LCGSNAGIAAILGTGSNSCFFSQDEIVKNVSPLGFILGDEGSGAVLGKKLIGDFLKNQ
                     MPKDLSDLFASTYNINAITTLDRVYKKPFPNRYLASFVPFMSENKSNKYVYNLIYDSF
                     TEFIVRNIKQYDYQKYPIHFTGSIAYNFKEILSEVVSKQNMKLGNITQNPIEGLVRFH
                     K"
     gene            complement(9480..11159)
                     /locus_tag="PK641_01090"
     CDS             complement(9480..11159)
                     /locus_tag="PK641_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG54984.1"
                     /translation="MAKIDRKEYTKEEVISKMTMKQLKKVARWLYDLSAPYKKKILFF
                     TIWGIINVGLGLLYIWLTKSIIDIATNAQIGNFWLIAFVLIALSLFQISAQYLVGYYG
                     AKFSVEVSNGVRYKMFATVLRSKWYEQEKFHTGDILTRIKQDASEVVGFLVSSFPSFV
                     ITFVQLIASFIFLFYLDKMLALIILCVSPIFFLFSKLYVFKMRKYSLQLKKIGSDMNS
                     MLQESVQHQVVVKTLEYQEPNLNQLGEIQDFFKFITLKRTKFGLNLRSVFTFGFSGGY
                     MVAFLWGVYQLKLSAISFGTLTAFLQLVNKIQGPVAGLIGIIPGFINVYTAVERLLEL
                     EDMSTEEKGDPVNLGEGISIDFTDVTFAYDKEPVLKSLNLHFEAGEFTAIIGETGCGK
                     TTVMRLMLALLEPQKGQILLKKGSRSEAVSALTRCNFIYVPQGNTLFSGTIRDNLRMA
                     KENATDKEMEDVLRVAAADFVFDLPQGIDTSLQEFGQGLSEGQAQRIGLARSLLRGGN
                     ILLLDEVTSAVDELTESKIIQNLRVFAKDKTVIFVTHRLKIADKCDKVIKM"
     gene            complement(11169..11438)
                     /locus_tag="PK641_01095"
     CDS             complement(11169..11438)
                     /locus_tag="PK641_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009235562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HPG54985.1"
                     /translation="MKLKDGFKLHQIDDECVILAEGIDNIDFSKMISLNESAAYLWRS
                     LEGKNFTEQTVTDLLLSEYEVSKDLASQDAKSFIQTLNDAGVLDL"
     gene            complement(11447..>12937)
                     /locus_tag="PK641_01100"
     CDS             complement(11447..>12937)
                     /locus_tag="PK641_01100"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPG54986.1"
                     /translation="FAQMLPLDAINQMAKKQEDEGLVVWIASPEKVSANYPDEAYVKH
                     LLDSISKAEITPYQEAVLNEPLIKELPQAGKIVSESMSKDSVYDYVLSNGVTVEFKPT
                     THKQDELLFTAYSKGGFAYENKADAITLKLINELASIGGLGSYNAIDLKKVLAGKNVS
                     VKIDIIDYSETITGSSSIKDIETLFQLVYLNFTNIRSDKDAFESYIARKEDYLKNMDA
                     YPIIELQDSLSSVFYNNHPLTTRTKASDVKNINYERALQILKQRFSNAADFHFIIVGN
                     ASPDSIKSFLTKYIAVLPANSAREQFKDAGTVAVKGNKTVHYKKQMMNPNTIAILEYT
                     GEIPYTLANIVATDALSQILYIVYTEKVREDEGGTYGVYPSVSLDAVPWNRFTAKIQF
                     STDSSKVDKLLPIIKNEFENIAKNGPREQDLQKVKEFMTKKYTDNKIDNGYWRQIMLE
                     QYITGINMNDKYLETLDKTDSKTIQKLAAKILKFNNRKEVVQIGTK"
BASE COUNT         4104 a         2314 c         2028 g         4491 t
ORIGIN      
        1 aatttaatta tgccatttcc attaagtcgg ttaaatcaag ttcgttttta tctttatcaa
       61 taaatttata ttgtaaaaat gaaaaatctt gattaggtct gactttaatc ccaaagccgt
      121 acttgaattt ccattttaat gccatacttg ggaaaccatg cttgatgtat gcgtatacat
      181 agggatgcac aaacaaggta aatttcttaa gttttaaatt atgatataaa atatctattt
      241 ttcctaccaa ggcatctgca aaaagcaagg atggacgtat agttccgttt ccataacaag
      301 ttggacatgt ttcttgagta tttattaaca tattaggacg tactctttga cgcgtaattt
      361 gcatcaaacc aaacttagtc aagggaagaa cgttatgctt agccggatcg ctttgcatat
      421 tttctctcat tttgtcgtaa atcttctgtt tgtgttctgc ggaattcatg tcaatcaagt
      481 cgattaccac aataccgccc atatcgcgca aacgtagttg ccgtgctacc tcatctacag
      541 ctaacatatt aacctcaaaa gcattttcct cctggagatt acctacttta gctcgtgtac
      601 cgctatttac atcaattaca tgcaatgcct ctgtgtgctc aacaactaaa tatgctcctt
      661 ttctgaatgg tacaatacgt ccgaaagagg attttatttg ctttgtaatg ttgaatatat
      721 caaagatagg cagttcatca tcataaaatt tgacgatatt ggctttttga ggctcaataa
      781 gactaatata tgcttttatt tcttcatata tagttcggtc gtttatataa atgctctcaa
      841 aatcaggatt aaaatgatcg cgcaaaattg actctgcacg gcctatttct tcaactacaa
      901 gtgatggagt tgatttggtt ttttgaattt taatcagcgt atcttcccaa cgcttaatta
      961 agacaagcag ttcgttttcc agttcacttt gctccctacc ctcagctaca gtacgtatta
     1021 tcagaccgta atttttgggt ttaacggctt gaagtatttt ttttaatctg ttacgttctt
     1081 cgttagaggt tattttctga gaaatggaaa ttttattaga aaaaggaagc aatactaaag
     1141 accttcccgc tattgaaatt tcggaagtta agcgaggtcc tttagttgat atcggttctt
     1201 ttgtaacttg aactaagatt tccttacctt gagtcaggat atcggttatc aagcctgttc
     1261 ttgctaattc gggtctgaac tgaaccttgt tcatcgaagg taacttcttt ttattagaag
     1321 tcataccatt agtaaattct gtaatggtag taaaatatgt acctaaatct aaataatgca
     1381 gaaaaccttc tttttcatag ccaacatcaa caaatgcggc atttaaaccc ggcatcagtt
     1441 tttttacttt ggccaaatat acattaccaa cagaaaatgt agcagaccga ctctccttgt
     1501 ttaactcaac aaggcgacta tcttccaata aagcaatgga aatttgctca ggttgaacat
     1561 ccaatatcag cttgctatcc atgtaattaa ttttcggctt tcagaaactt cgtacgaagt
     1621 gttttatatg aatctagtaa tacagaaaag aacaaactct atataatgaa gtttgttctt
     1681 tattgtttcc cgattcttga tttataaatc aaagaattaa agacttactt tttcttctta
     1741 tgacgatttt ttctcagtct ttttttacgt ttgtgtgtag acatcttatg tctttttctt
     1801 ttctttccgc tcggcatagt tatattattt taaaaaaatc ttaagtactt attttttaac
     1861 agactcaata aatgtctttg caggtttgaa tgatggaata acatgagcag ggattgtaat
     1921 tgtaacattc tttgaaatat tacgagctac tttttctgca cgttgtttta caacgaagct
     1981 tccaaaacct ctcaaataaa cattttgttt cgaagccaaa gacttcttta cttcttccat
     2041 aaaagactca accacctgca taacagcaac tttatctatt ccggtggtgt ttgaaatttc
     2101 atttacaatt tcagcttttg tcatatctgt taatattttg tgtttataat attttaaatt
     2161 caaaaacgga gtgcaaatat aatactttta ttatcaagtg catatgtttt acaaaagaat
     2221 tttggttttt ttttgaattt atctgttttg tgatatatag attcaagtag gaagtgcgaa
     2281 attacgaata tttttaaaga aagccgattt aggattaata aaatttaatt tctttcttgg
     2341 tctcctattg attttgaatg caattgaagc acgactaaaa attgccaata atggcgtaaa
     2401 ttatgaatag aatgatagaa ataaaaagag gctttggcag taacgcattg aaaatgaaaa
     2461 aaaatgcttt actttgcaag attttaaaaa ataacaatta ttatatagac agtgcatatc
     2521 tatataacta aaaatgcaat aataaaattg catcatgaat aattaataat gaagcgaatt
     2581 acttttatat ttatatgtgt cattttaagt tgctctgttt cttttggaca attaaagaaa
     2641 agtagaatcg gttctaatag caaacaatat gtaagtttgt cgggaggtgc caataagcct
     2701 ttaatggata tccatacgga tttcgaacct tcatgttctt tttcttatac atattatttt
     2761 ttacctaaac taggacttcg tggagcttta gaatacaatt ggtttacagg gcaatctccg
     2821 gttggacatc ctaattatta tgcacacatt gtagaactta gtgcacaagt tgtctttaat
     2881 tattatgttt atacaggtag tagaagagaa aataattccg tttcgtttat tccggaaaga
     2941 gcatatattt ttggtggtat tggtgcattg tgctatttac gcgagcaagt aaattatgca
     3001 cctgcgttta ctgcatcatt tccaataggt accggtttac aatttccaat acgcgataat
     3061 tgggcttggg gaattgattt tacatggcat tttacaatag ccgatgatat agacaattat
     3121 ccggtccaaa aatcttttac agacggatac cctactctga tgttgacatt tacttacaaa
     3181 atacctgacc aaaaacgaag tggtaccggt tatggataca aatcttctaa gaaaggatta
     3241 aaatgcgacc ctcgtaaagg ttgtgaattt accttcgatt aatttattta attccgcgtt
     3301 tatttaattc cctttgatat ttacgtgcat tacggctatg ctcttggaat gttaccgcat
     3361 attcgtgtgt acctgaaaaa tcggattttg cacaaatata taagtatctg tgatgcacat
     3421 aatttaaaac tgcatctata cttttttcgg aaggaatacg aataggaccg ggaggcaaac
     3481 ctgcattttt atatgtatta taaggagact ctatttctaa atgtgttttt aaaattctgt
     3541 ctatagtaaa atcaccccaa gcaaagcgta ctgttggaca ggactgcaat ggcattcctt
     3601 tatgaattct gttaatgtat acgcctgcaa ccatgcattt atcttcatat tcattagttt
     3661 cttcttctat tattgatgct aatatatgta tttgaggtat tgttaaacct gtttttgatg
     3721 ctaatgccct acgctcggga ttccaaaatt tatcatattc tttagtaatt tctgcaacga
     3781 tttcggcagg tgatgcacac catgacattt cttttgagaa tcttataaat agagctaatg
     3841 agttatcttt atttaaatta tagcgcaaaa gtaaactgtc tgataacagg taatttgcca
     3901 aagatgcaga atcgacctcc atttgtctac tcaaattgcg tgcaacccac tcggtatatc
     3961 gagtcatttt aagttctatg ttaacatgat gcgacattcc ataagcaagt ctgttgatga
     4021 atgctctatt agaaatattg ggttcgagca catacttacc acacatagct tgcttttttt
     4081 tgcctattaa ttttaatgca aaatcgaagg atttttctga ttttattaag ttgttagatt
     4141 tcaatgaatc cttcacagac tgccatgttg aatttttggg cacataaaaa tcaatatatt
     4201 ttttttgact tgtttgaata ttaggatatt gcaaaaaata atatgcgcct gcactaattt
     4261 caagtattga cacacaaacc agcaatacaa ttatcagata aatatttttt ttgttcatta
     4321 tattttaaaa tatcaaatca tttgactaca tttgcacccg caacggcgat agaacctgca
     4381 tggaagtgtg ggtgagtggc tgaaaccacc agtttgctaa actgacgtac gggtaactgt
     4441 accgggggtt cgaatccccc cgcttccgca tcaaatccct ttagattatt ctgaagggat
     4501 ttttgtttta ttaaactgta tacgagccat tatttctata gtacgtaatc tatctttata
     4561 taaattattc ttgtttaatt tatcaatatt gagtgatgca tctgaattgc cttcccaacg
     4621 acggcaaaga tacaatgaat tatatattct tcctatttta tactctcgac tgattctcaa
     4681 gcccatagca taatcttcac cataactggt atttggtaat tgtatggaac gcaacaaggg
     4741 tgtataaaat gcacgaggtg cacctaatcc attgatacgc aaagcattat tatgtccatt
     4801 ctcattagtc cattctttgt gatcaatcaa accgggaggc aagatcttca tatcaaaacc
     4861 agtaatagta tatgagccga tcaacattgc acaattttga gcataaaatc catcaataat
     4921 tgtttgcaaa gtatgttggt cttgatatac atcatcgcta tcgagttgaa tggcaaactt
     4981 accgcatttt gcattattaa ttccacaatt ccagcatcca ccaataccta aatccaaacg
     5041 ctcgggtact atatgcacta ctctattatc agattttgca aatctctcta ttatttcgga
     5101 agttccatcc gtggaatgat tgtcaataat caataaatta aacgggaaat ttgtggcttg
     5161 actcaggact gattcaattg catctgctat tgttttagct ctgtttttaa caggtataat
     5221 aactgatgcc tgattttcga attgtgcatc atcaaaagaa atcttcttaa aatcggctgt
     5281 cagatatgct cctattgatt taagatagtt agtacatgct gattccatct caatttgcac
     5341 agctctattt ttgggatcta cataattaaa ttgcttttct tcactactaa ttttatcttc
     5401 ttcaaattcg gtgtacaaga attcgtttaa atgaataatc gggaattttt tagctgtttg
     5461 cagacgtaaa tcgtatagtg ctgcatatag ataatctgta gcaatgcttg tttttaaggc
     5521 atctgccgag aataaaatta caggaccgaa atcaaaatca tctcgtacac tgcctaattg
     5581 atagtcgatt gttggtacgg cttctttttg gtctcctttt acatgataat aatcggaata
     5641 caacatacct gctcctgttg atgatgctac atcaatcatt ctttttatag cattctgacc
     5701 taaatcaaga gcagtttttt ttgtatatac tatggcatat ccatcaggag tatgtgtacg
     5761 tatataatca attgcatcat gatttgttgg aggtgatggt aaaacataag ttttatcaac
     5821 tccttctata ttaagattat ctaataaaga gaaaaaacgt gctttcatat attattaata
     5881 attcaaaaat aaacgcataa cttctgtacg ttgtgaatcg gatgttaaag cttcaatagg
     5941 aaatccggca gcaaccactt tatagttacg attaaatcct aaggctgctg aaactttatt
     6001 ttcttcataa ctaagcaaga cttcagctat tttatccatc tgttgaagcg catcaacatt
     6061 ttgtaagaaa tattgactgg aattagcttg catttggagt gtgtaaatac tttcattgtt
     6121 tggggtgatt attactccat ttgtacttgc ccatggtgca cgcaatttat aacgcaatgc
     6181 tttttctgtg aacaagcatg attctgtgcc tatatatgca cctgttatta tgactttttt
     6241 atttgctttt ttaatatatc gagtgatggc cgattgtaac ttatgggggt ataactcgta
     6301 gccatcaacg gttctctgtt ttcccataat aatatcgagc acatcataac ttgacaaatt
     6361 aatatctcca ctcataacag catcttttcc ggatgataca aatgaatagc cattatcata
     6421 aatagcttta ccgtgaacat aagcataatc aaaggtatta ccggctatga tttgtgtctc
     6481 ataatttgat ctgcttgcac caaaaccggg ataatcatta ctttgatatt ctattgattt
     6541 atcaaactca atctgatttc ccgtatatgc tatgtcatac atatatggaa ctccgaaatc
     6601 acgatcatag aggaatccgc cttgcgaacc gcaatcaaaa gattccggac cacctatacg
     6661 atcgaaacca ttaataatca ttacagtacc tttagattct gatttattgc acactgacag
     6721 tatttcagaa gggaaacttt ctccaccttc gtttgatgcc gttaccttga aactataaat
     6781 agtatcgggc ttgacagcta aagaaattga atttgatttg actatctgtc cattatcaaa
     6841 actaccgtca tttttacgag tatataatat atatcgaaca ggatatgcag tttcttccaa
     6901 actatctgac acaggtttcc aacttaaact aactgagtca tttgctaatt cagctccaaa
     6961 agatcttacc ggcaatggtt gaacaacata actttgacca ttatgttctg ctaaaaattt
     7021 aaggactcct ttatatactg atctggctaa taaaaattta aattgaggct gtaagccata
     7081 caacatatct tgataattga gatgcgaaaa caactcgatt attacagatg gtactgccgg
     7141 tacgagtgtt tcaacatatt tttgatgcca aataccacga gacgaccaat ttttacattt
     7201 taattttgat acatcgtcaa taatttgagt acgtattcta tctgccaaat catgacctgc
     7261 catttttgaa tatcctgaag gatacacact atctgatgta cataccaata atgttcctac
     7321 aacgctatcg gctcccattg taaatgcatc agtatgaaca gctaaaactg catcaatagg
     7381 aatttttaag ccgggatatg agggatacac atgcgaacct ccggctaaga agttaaccca
     7441 ttttgaacgc gaatatatat catcataata atcgctctta tagcccggtt tttgatcata
     7501 taaaatacta tccggcaagc ctgcttgttg caaattatat cttgctgcct cggcaaatct
     7561 tggatatcca tttccgaaat gactgatacc gcctcctatt tttactgaat cgacacacag
     7621 cgcatctttt ttgtcagcca taacgaaact tacacgtaaa tcattattga aatataggcc
     7681 atcaatatct atccatgttc cggcacccat gtttgcatta atcttataat tatttacaat
     7741 accggcatgc tcaattttac aaagtatttg tcctgatgcc ttagtctcct tcccatacca
     7801 tattttaatt ctgtatgttc cggctctgtt tactgttccg gtaaattctg agctatcgga
     7861 agatattgtt gttttgcaca agtacaattc ttccatttgt atgtcacgtt cacgaggcat
     7921 aaaaacttgt gcacctgaat tttctaacat tggtactaaa aacggaatta caattgaagc
     7981 agtcaacaag tcttctactg taccaaacaa gcgaggtctt tgccactccc agcgattttg
     8041 ggtacgatta aagtacaaac catgactacc ccacaatgca atatttctgt cggttaagcc
     8101 ttgtgttgga aataataatt gagattcttt ttttaccaca gatcccccac tcttatagtt
     8161 gggcatacgc gatttatcaa tcggaagatt ctttctcaaa aaatttggga caaactcttc
     8221 aatatatttg tttcgagaaa taattgacaa ttcgtatgat gcatattcag aaggtaattg
     8281 aactcgcaaa gaatcatata catccgggag catctcttct ctaaaaataa gattagaaaa
     8341 ttcgtcttct aaatatactg ttaatttgtc ttttgaaaca actgtttttg taactgaata
     8401 ttcttttttg ttggtaacaa tgttaggtaa atagcgaaat ttagtaataa gcggtaatga
     8461 gggctttgct gctaaaagaa tttcagcagc cataaaaaac aataccagta ttgataaacg
     8521 tgcgggtttc atatatctcg taaaagtaca gcatttttta ttcatttcat agtgataaat
     8581 tcaaaaaaac accttaaatt gcattcttat tacaaacaat aatggtgata caaatatgaa
     8641 attaattgca gatagtggtt caaccaagac ttcgtgggta ctaacaaaaa agtctaatat
     8701 tattcaacaa attacaactg acgggatcaa tccgtattat caagacgaaa ataaaatatg
     8761 tgaaactatc aaagagcaat tatttactaa aattgaaagc atcaatattg atgaaatata
     8821 tttttatggc gcaggatgcg catcgcctga aaagaataaa atgattgctg atgcccttgg
     8881 acaatttttc ccgacatcta tcattgctgt taattccgat ttacttggtg cagctagaag
     8941 tttatgcggt agcaacgcag gtattgctgc tatattagga acaggatcaa actcatgttt
     9001 cttttcgcaa gatgagattg ttaagaatgt ttcgccttta ggttttattt taggtgatga
     9061 aggtagcggt gctgtattgg gtaaaaaact aattggagat tttttaaaaa atcaaatgcc
     9121 aaaagaccta agcgatttat ttgcaagcac ttataatatt aatgcaataa ctacattaga
     9181 cagagtctat aaaaagccgt tcccaaacag atatttagca tcttttgttc catttatgtc
     9241 tgaaaacaaa agtaataaat acgtttacaa tttgatttat gacagtttta ctgaatttat
     9301 tgtgagaaat atcaagcaat acgattacca aaaatatcct atacatttta ccggttctat
     9361 tgcttataac ttcaaagaga tattatcaga agtagttagt aaacagaata tgaaacttgg
     9421 aaatataact caaaacccta ttgaaggttt agtaaggttt cataaataaa caaactgttt
     9481 tacattttaa tgaccttgtc gcatttatct gcgattttta aacggtgggt aacaaatatg
     9541 actgttttgt ctttggcaaa gactctcagg ttttgaatta ttttggattc tgtaagttca
     9601 tcaaccgcag aagtaacctc atctaaaagc aaaatattcc caccgcgtaa taatgaacgg
     9661 gcaagaccta tacgttgtgc ttgaccttcg gataaaccct gaccgaattc ttgcaaactt
     9721 gtatctatac cttgtggcaa atcaaacaca aaatcggctg ccgcaactct tagcacatct
     9781 tccatttctt tgtcggtagc attttctttt gccatgcgaa gattatctcg aatagtacct
     9841 gagaatagtg tattgccctg aggtacataa ataaaattac aacgtgttaa ggcagaaaca
     9901 gcttctgaac ggcttccttt cttaagtaat atttgacctt tttgaggctc taacaaagct
     9961 agcatcaaac gcatcacagt agtctttcca caaccggttt cgcctattat tgcggtaaat
    10021 tctcctgctt caaaatgtaa attaagcgat tttaaaacgg gttctttatc gtaggcaaaa
    10081 gtcacatctg taaaatcaat agatatgcct tctcctaaat ttacaggatc gcctttttct
    10141 tctgttgaca tatcttccaa ttcgagtaaa cgctcaacgg ccgtataaac attaataaag
    10201 cccggtataa taccaattaa gccggcaaca ggaccttgaa tcttatttac taattgtaaa
    10261 aatgcagtta aagtaccaaa actaatggca cttaatttta attgatatac accccataaa
    10321 aatgcgacca tataaccgcc actaaatccg aaggtaaaaa cactacgaag atttaatcca
    10381 aattttgttc tctttaatgt gataaattta aaaaagtctt gaatctcgcc taattgattt
    10441 aaattaggtt cttgatactc taatgtttta accacaactt gatgttgtac agactcttgc
    10501 aacattgaat tcatatcact tcctatcttt ttaagttgca aagaatactt acgcatctta
    10561 aaaacatata atttgctaaa caaaaagaat attggcgata cgcataaaat aatcaatgcc
    10621 aacatcttat ctaagtaaaa cagaaaaata aaagaagcta ttaattgtac aaatgttatt
    10681 acaaaagagg gaaaagaaga tactaagaaa ccaactactt ctgatgcatc ttgtttgatt
    10741 cgagtaagta tatcgcctgt atgaaatttt tcttgttcgt accatttgct gcgcaaaaca
    10801 gttgcaaaca ttttataccg tacgccattt gaaacttcaa cactaaattt tgcaccataa
    10861 taaccaacaa gatattgagc tgatatttga aataaactca atgcaataag aacaaatgca
    10921 ataagccaga aattgccaat ttgagcattc gtagctatat caattattga tttggtaagc
    10981 caaatgtaca ataatccaag accgacattt ataatacccc atattgtaaa gaataaaatt
    11041 ttctttttgt atggtgcaga taagtcatat aaccaacgag caactttttt aagttgtttc
    11101 attgtcattt tacttatgac ctcttctttg gtatattctt tgcgatctat tttagccatg
    11161 tactggttct acaaatctaa cacaccggca tcgttcaatg tttgaataaa agacttggca
    11221 tcttgcgacg ccaagtcttt tgaaacttca tattctgata aaagcaaatc tgttactgtt
    11281 tgttcggtaa agttttttcc ttccaaacta cgccacagat atgctgctga ttcgtttaaa
    11341 cttatcattt ttgagaaatc gatattatca attccttcgg ctaaaataac acactcatcg
    11401 tcaatctgat gaagtttaaa tccatctttt aatttcatag atatacttat tttgtaccta
    11461 tctgaacaac ttccttacga ttattaaatt tcaagatttt agctgcaagt ttttgaatgg
    11521 tttttgaatc ggttttatcc aaggtttcta agtacttatc attcatatta ataccggtaa
    11581 tatattgttc aagcataatc tgtctccaat atccgttatc aattttatta tcagtatatt
    11641 tcttagtcat aaactctttc accttttgta aatcttgctc acgaggacca tttttagcaa
    11701 tgttttcgaa ctcatttttt ataatcggca atagcttatc tacttttgat gaatcggttg
    11761 agaattgtat cttagcagta aaacgattcc aaggaactgc atccaaacta acacttggat
    11821 atactccata agtaccgccc tcatcttcgc gaactttctc ggtatatact atatacaaaa
    11881 tctgagacaa ggcatctgtg gcaacaatat tagctaaagt gtatggtatt tcaccggtat
    11941 attctaatat tgcaatggta tttggattca tcatttgctt tttataatga accgtcttat
    12001 tcccttttac agctacagta cctgcatctt taaattgttc tcttgcagaa tttgcaggta
    12061 aaacagctat gtactttgtc aaaaatgatt taatactatc cggtgatgca tttccgacta
    12121 taataaaatg aaaatcggct gcattagaaa aacgttgttt taaaatttgc aatgcacgct
    12181 cgtaattaat attttttaca tcagaagctt tagtacgagt tgttaatgga tggttattat
    12241 aaaagacaga gctcaatgaa tcttgcaatt caataatagg atatgcatcc atattcttta
    12301 aataatcttc tttacgtgca atgtatgatt cgaaagcgtc tttgtcgctt cgtatatttg
    12361 taaaattcaa atataccaat tgaaataatg tttctatatc tttaatagat gatgaacctg
    12421 taatagtttc gctataatca attatatcaa tttttacaga aacattctta ccggctaata
    12481 ctttctttag atctatagca ttataagagc ctaagcctcc tattgaagct aattcattta
    12541 ttagttttaa tgtaatagca tctgctttgt tttcgtatgc aaatccgcct ttactatatg
    12601 ccgtaaataa caactcatct tgtttatgag tagtaggttt gaattcaact gtaactccat
    12661 tagacaagac ataatcatat acagaatctt tgctcatgct ttcagaaact attttaccgg
    12721 cctgtggcaa ttctttaata aggggctcgt ttaacacagc ttcttgatat ggcgttattt
    12781 cggcttttga tatactatcc aacaaatgtt ttacgtatgc ttcgtcagga tagtttgcag
    12841 atactttctc gggtgatgct atccatacta caaggccttc atcttcttgc tttttggcca
    12901 tttgatttat tgcgtctaat ggcagcatct gagcaaa
//
LOCUS       DAOPID010000013        12768 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_5791_length_12768_cov_4.268544, whole genome shotgun sequence.
ACCESSION   DAOPID010000013 DAOPID010000000
VERSION     DAOPID010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 12768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12768
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5791_length_12768_cov_4.268544"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PK641_01105"
     CDS             complement(<1..289)
                     /locus_tag="PK641_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HPG54987.1"
                     /translation="MAQMNFGGVIENVVTREEFPLEKAREVLKNETIAIIGYGVQGPG
                     QSLNLRDNGFNVIVGQRKGKTWDKAVADGWVPGETLFEIEEACQRGTIIQYL"
     gene            complement(405..1142)
                     /locus_tag="PK641_01110"
     CDS             complement(405..1142)
                     /locus_tag="PK641_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HPG54988.1"
                     /translation="MNKDELTQTFIHHIGPQEVDCSGYMSLTALGSAILNTAGDAAEK
                     HNFGMNFLLKEGLTWVVSRLSISIDKLPKMYDVIKIETWIKNYGRVFTIRNFKIYNSK
                     REEICKASSQWAVIDLKTRRPFDLRNKPEWQQYATEESRGVEDPIGISAISGELISEH
                     RVRFSDIDFNQHTNSMKYAEWMLDSYSPDTLKNRSITQFDINYMHEALYGQNIKIVRS
                     ENENQSLFNLTDNDNFSVCRAAIRWRL"
     gene            complement(1148..1696)
                     /gene="ilvN"
                     /locus_tag="PK641_01115"
     CDS             complement(1148..1696)
                     /gene="ilvN"
                     /locus_tag="PK641_01115"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HPG54989.1"
                     /translation="MMENKLYTVTVFSENTPGLLNQITIVFTRRQINIETLSVSPSAI
                     KGIHKFTITVVTDLDSIKKVVKQIDKRVDILKAYYNTDDELIYQEIALFKLATDKVLA
                     MGGIEYLIRKYNARVLEILENCVVIEKTGLYQETQEMFDELQKAIGVLQFIRSGRVAI
                     TRGRVERLSDMLAGIDSKMKNK"
     gene            complement(1697..3400)
                     /gene="ilvB"
                     /locus_tag="PK641_01120"
     CDS             complement(1697..3400)
                     /gene="ilvB"
                     /locus_tag="PK641_01120"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HPG54990.1"
                     /translation="MANTMTGAYALMEALLNEGTDTIFGYPGGQIIPVFDALYDYKDK
                     LRHILVRHEQGATHAAQGYARVSGRPGVVIITSGPGATNAITGIADAQMDSTPLVVIA
                     GQVPAGILGTDAFQETDLVGITQPITKWSYQIRDPKEIAWAVARAFYIAKSGRPGPVV
                     LDFTKNAQFSQVDYKPEVCNFIRSYIPVPEMNPEKIKAAADLINNAKKPFALIGHGVT
                     IGQAEDELMQMLDKADIPAAWTLLGESAMPTDYHLNKGMLGMHGNMGPNLKTNECDVL
                     IAIGMRFDDRITGNLNTYAKQARIIHFDIDPSEIDKNVKTDLAVIGDVKATIPAVTEL
                     IKEAKHTEWIASFDAYYKQELNSVIKNDLNPSSDSPIKMAEVVRKISDATNREAILVT
                     DVGQNQMVAIRYFQFSKKRSVVTSGGLGTMGFGIPAAIGAKIAAPDRTVCLFCGDGGF
                     QMTLQELGTIMQENLGVKIIILNNNYLGMVRQWQELFFHKRYSYTPMINPDFQAIASA
                     YRIKSKCVSKRDELDNAIAEMIADDKAFLLIVNVEEAGLVYPMTPAGGSVTDILLGEE
                     N"
     gene            complement(3403..3837)
                     /locus_tag="PK641_01125"
     CDS             complement(3403..3837)
                     /locus_tag="PK641_01125"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HPG54991.1"
                     /translation="MKLIMLIKEIIGKQPAHSTLVQICRYICSGGLAFAFDYVTLYVL
                     TEYVGFHYLVSSACGFSVGLVITYILSIYWIFDEHRTENKIVEFSVFLLIGLSGILLN
                     IGLMWVFTSLCSIYYMLSKLITTVIVFVWNFVLKKKLLFTHR"
     gene            complement(3834..4574)
                     /locus_tag="PK641_01130"
     CDS             complement(3834..4574)
                     /locus_tag="PK641_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPG54992.1"
                     /translation="MLNGKKIVVVMPAYNAEKTLEQTYKEIPFDIVDEVILTDDCSKD
                     NTIAKAKELGIKEVLSHEKNKGYGGNQKTCYNRALELGADIVIMLHPDYQYTPKLIQS
                     MSYMIANDLYPVVMASRILGMGALKGGMPIIKYISNRFLTLFENILLHQKLSEYHSGY
                     RAFSAQVLRDINYNVDSDDFIFDNEMLAQIFMKGYEIGEITCPTKYFDDASSINLKRS
                     TIYGLGVIRVSIIYRLCKWKLMHSKLFA"
     gene            complement(4576..6237)
                     /locus_tag="PK641_01135"
     CDS             complement(4576..6237)
                     /locus_tag="PK641_01135"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPG54993.1"
                     /translation="MSAKTENILIQIGIAIAAALFFIPGIGSVHLFDWDEINFAESAR
                     EMITSGNYLTVQINFEQFWEKPPLFIWMQVVSMKLFGINEFAARIPDAICGIITLLVL
                     YNIGSKIRDRRFGLLWTMLYACSLLPFMYFKSGIIDPWFNLFIFLGTYYFIVFTDPGN
                     SKNINIKLVASAFFLGLAILTKGPVGLLIFCLTFGVYLITNNFKLNFKWHQAILFLII
                     LGLTGGFWFILQIIDGNFSIIQDFIIYQIRLFETKDAGHGGFPLYHFVILLIGVFPAS
                     ILSLPAFNYKITKTEKDASFKRFYKWMMILFWVVLILFSIVRTKIIHYSSMCYFPLTF
                     IAAFACERYINGKMVMPKYVKILLIIIACLLGLAVALVTQFDNFKHLLYPYILDEFAI
                     GNMQASATWYGFEFAFSIILIIGVVWMCIKSNKILSYKNLCIMCASCIIFIYSALVFI
                     VPQVEKYSQAAAIEFYESKKAEDCYIYLPYFKSYAQYFYSDRQIKNKNADASYLMHGQ
                     LDKDCYFVLKDISSEISIFESEVKDAKLINKKNGFAFYVRKLQID"
     gene            complement(6244..8076)
                     /gene="ilvD"
                     /locus_tag="PK641_01140"
     CDS             complement(6244..8076)
                     /gene="ilvD"
                     /locus_tag="PK641_01140"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005847233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HPG54994.1"
                     /translation="MKHSLRSDQCTQGRRMAAARALWRANGMKPNQVGKPIIAVVNSF
                     TQFVPGHVHLHEIGQEIKAEIESLGCFAAEFNTIAIDDGIAMGHDGMLYSLPSRDIIA
                     DSVEYMVNAHKADAMICIRNCDKITPGMLIAAMRLNIPAVFVSGGPMEAGEWKGQHLD
                     LIDAMVKAADTSVSDKDVNEIEHSACPSCGCCSGMFTANSMNCLNEALGMALPGNGTI
                     LATHGNRKRLAMDAAKQIVKNAFAYYQDGDESVLPRSIATRNAFLNSMTLDIAMGGST
                     NTVLHLLAIAHEAEVNFTMEDIDQLSRKTPCLCKVAPNTTKYHIQDVNRAGGVLGILN
                     ELAKGGLLHTEAKRVDGMTVGEVIKAYDICSGNISDDAKRIYSSAPCNKFNLELGSQN
                     ATYDSYDTDRAEGCIRDMAHAYTKDGGLGILKGNIAMDGCVIKTAGVDESLWKFSGKA
                     VVFNSQDSACEGILGNKVQAGDVVVITHEGPKGGPGMQEMLYPTSYIKSRHLGKDCAL
                     ITDGRFSGGTSGLSIGHISPEAASGGNIGLVKDGDIIEIDIPARSINVKLSDAELAAR
                     RKEEEARGKDAFKPKGRNREISKSLKAYASLVSSADKGAVRLID"
     gene            8509..10257
                     /locus_tag="PK641_01145"
     CDS             8509..10257
                     /locus_tag="PK641_01145"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007486519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HPG54995.1"
                     /translation="MENNLLLDVRKKAEIWLSGDYDQDTKTQVKALLDNEDTTDLIES
                     FYKDLEFGTGGLRGIMGVGTNRMNIYTVGAATQGLANFLKKEFGKGKQIKVVVGYDCR
                     NNSRLFSETIANIFSANGIKVYLFEDLRPTPEISFAIRELGCQSGVVVTASHNPKEYN
                     GYKAYWEDGAQVIPPYDTSIISEVNKIKHIDDIHFKGNPELIEIIGENIDNLFLEKIQ
                     KLSLSPESISRNKDMKIVYTPIHGTGITLIPKALKMYGFNNVIDVPEQNVISGDFPTV
                     KSPNPEEPAALKLAVDKARSVDADIVMASDPDADRMGIAVKDDKGEWILVNGNQTAQI
                     LVYYLIRRYKELGRLKGKEYIVKTIVTTELIAEIAKRYKVPYFDCYTGFKWIAAIIRE
                     QEGKRQYIGGGEESYGFLVEDFVRDKDAVSSCVMMAEIAAWAKDNGKTVYELLQDIYL
                     EFGFSKEKGISVVKKGKTGAEEIKKMMTHLRSCPPKTIAGSKVTIIKDYNALRMIDLA
                     AGSTTNMEMPETANVLQFFTEDGTKISVRPSGTEPKIKFYIEVRLPLKSREEFDAVDA
                     AAEEKILAAKKSLHLD"
     gene            complement(10328..11260)
                     /locus_tag="PK641_01150"
     CDS             complement(10328..11260)
                     /locus_tag="PK641_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HPG54996.1"
                     /translation="MRTFLKNGNGLIPCQQWEPNCWVNVVKPTQNDADYIINELHVPS
                     AFYEDIEDMDERPRIEVEDGWCFILMRIPCKEMEDPNSVPYTTVPLGIIFKDDVFVSV
                     CFYQTDLITDFIQYTKRKNIERTDNLNFVFRLLLSSSIWFLKYLKQINNRTKIAEKQL
                     ENSIRNEDLQALLKLEKCLVYFTTSLRGNEILTHRLKNLRNTKDLYDPELVEDVEIES
                     KQALEMTNIYSDILSGMMDAYASVISNNLNVVMKRLTTISIVLMMPTLIASYFGMNLE
                     SGTTIFNWTFLIVIITSILITAFTTGFFIKKRWF"
     gene            complement(11599..12501)
                     /locus_tag="PK641_01155"
     CDS             complement(11599..12501)
                     /locus_tag="PK641_01155"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HPG54997.1"
                     /translation="MKKIKVAIIGYGNIGKFSVEAALAAPDIELCGVVRRASSLSDNT
                     ADLAGIKVVSNIDELGKVDVAILCGPTRIVPEIAVSILKKGICTVDSYDIHGALWDLH
                     QQLDEVAKANGVASIISAGWDPGTDSVLRTLFQAMVPKGITYTNFGPGMSMGHSVVAN
                     SKSGVKNALSMTIPLGTGIHRRMVYVELEKGASIAEVTAAIKADSYFAHDETHVIEVE
                     SVDSIKDMGHGVLLERKGVSGKTQNQRISFSMSINNPALTAQILISCARAVVKQKPGA
                     YALPEIAPMDLLSGDRETLIKSLV"
     gene            complement(12519..>12768)
                     /locus_tag="PK641_01160"
     CDS             complement(12519..>12768)
                     /locus_tag="PK641_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPG54998.1"
                     /translation="ELIDVDTVIVSVGVSPNPLIPNSIPGLEVSKKGTIVVNENLQSA
                     IPMIFAGGDIVRGGATVILAMGDGRKAAASMDEFIQKK"
BASE COUNT         3974 a         2380 c         2120 g         4294 t
ORIGIN      
        1 ataaatattg tatgatagta ccacgttggc aagcttcctc aatttcaaat aaagtttcac
       61 caggaaccca tccgtcagca actgctttat cccatgtttt acctttacgt tgaccaacta
      121 taacgttaaa accattgtcg cgtaagttta atgattgacc aggaccttgt acgccataac
      181 caataattgc gatggtttcg tttttcaata cttcacgtgc tttctctaaa ggaaactctt
      241 cacgggtaac aacattctcg attaccccgc caaaattcat ttgtgccata attattatga
      301 attaaatatt taaagtttaa actgtctaaa acttacaatt gagctgcaaa ggtacgtttt
      361 ttttattaaa agtaaacttt tttgttgtat tctatgttta aatattacaa tctccatctt
      421 attgctgctc tacatactga aaaattatca ttgtcggtta aattaaacaa tgattgattt
      481 tcgttttcgg aacgaacaat tttaatattt tgcccataca gagcctcgtg catatagttt
      541 atgtcaaatt gcgtgatact tctatttttg agagtatcag gcgaatagga gtctagcatc
      601 cattctgcat atttcatact gttggtatgc tgattaaaat caatatcact gaatcttaca
      661 cgatgctcag atattaactc tccactgatt gcagatatgc ctatggggtc ttcaactccg
      721 cgtgattctt cggttgcgta ttgttgccat tccggtttgt ttctcaaatc aaaaggacgt
      781 ctcgttttta aatctattac agcccattgc gatgatgctt tacatatttc ttctctctta
      841 ctgttataaa tcttgaaatt tcggatagta aatactctgc cgtaattttt tatccaagtt
      901 tcgattttta taacatcata cattttagga agtttatcaa tgcttataga taaacgcgaa
      961 acaacccaag taagaccttc ttttaacaag aaattcatac caaaattgtg cttttcggca
     1021 gcatcaccgg ctgtattcaa aattgcagaa ccaagtgcag tcaaagacat atatccggaa
     1081 caatcaacct cttgcgggcc aatgtgatga ataaatgtct gagttaattc atctttgttc
     1141 actgcaatta tttgtttttc atttttgaat ctataccggc aagcatgtca ctcaagcgtt
     1201 ctacacgtcc acgtgtaatt gctacacgtc ctgaacgtat aaattgtaag actcctatgg
     1261 ctttttgtaa ttcgtcaaac atctcttgag tctcttggta caaaccggtt ttttcaataa
     1321 ccacacaatt ttcaagaatt tctagtacac gagcgttgta tttgcgtatt aaatattcga
     1381 tgccacccat agccaaaacc ttgtctgtag ctaatttgaa taaagctatt tcttgatata
     1441 tcaactcatc atcggtatta taataagctt ttaatatatc aacacgctta tctatttgtt
     1501 ttactacctt ttttatacta tctaagtcgg taactacagt tatggtaaac ttatggatac
     1561 ctttaatggc agatggagat actgataatg tttcaatatt tatttgccta cgagtaaata
     1621 caatggtaat ttgatttaaa agacccggtg tattttcaga aaaaacggtt acggtatata
     1681 atttattttc catgatttag ttctcctctc ctaataaaat gtcagtaaca gaaccacctg
     1741 ccggtgtcat aggatataca agaccggctt cttcaacatt aactatcaac aaaaatgctt
     1801 tgtcatcggc aatcatttcg gcaattgcat tatctaattc atcacgcttc gaaacgcatt
     1861 tgcttttgat tctgtatgca ctagcaatag cttgaaaatc aggattaatc ataggcgtat
     1921 atgagtaacg tttgtggaag aataattctt gccattgacg taccatacct aagtaattgt
     1981 tattcagaat tatgatttta acacctaaat tttcttgcat tatagtgcca agttcttgta
     2041 gggtcatttg aaaaccacca tcgccacaga acaagcatac agtgcgatcg ggtgcagcta
     2101 tttttgctcc tattgctgca ggtataccaa atcccatggt acccaaacca cctgatgtta
     2161 ctacgctgcg ttttttgcta aactgaaaat atctaatggc aaccatttga ttctgtccta
     2221 catcggttac caatatggct tctctatttg ttgcatcaga tatttttcta acaacttcag
     2281 ccattttgat tggactatcg ctgcttggat taaggtcatt ttttattaca gagttcaatt
     2341 cttgcttgta ataagcatcg aatgatgcaa tccattctgt atgttttgct tctttaataa
     2401 gttctgttac agcaggtatc gttgctttta catcaccaat tacagcgagg tctgttttta
     2461 cattcttatc tatttcggaa gggtctatat caaaatgaat gattctagct tgtttggcat
     2521 aggtgtttag atttccggtg atgcggtcgt caaaacgcat acctattgct attaaaacat
     2581 cacattcgtt agtttttaaa ttaggaccca tattgccatg cataccaagc atacctttgt
     2641 ttaaatgata atctgttggc atagcagatt cgccaagtag tgtccatgct gcaggtatat
     2701 cggctttgtc aagcatctgc attaattcgt cttctgcttg tcctatagta acaccatgtc
     2761 cgatgagggc aaatggtttt tttgcattat ttattaaatc cgcagcagcc tttatttttt
     2821 caggattcat ttcaggtaca ggaatatagc ttcgtatgaa attgcatact tcaggtttgt
     2881 aatcaacttg tgaaaattgt gcattttttg taaaatcgag tacaacagga cccgggcgtc
     2941 cgcttttagc gatataaaat gctcttgcaa cagcccatgc aatttctttt gggtcacgta
     3001 tttgataact ccattttgtt attggttgtg tgatacccac taagtcagtt tcctgaaaag
     3061 catcagttcc taatatgcct gccggaactt gacctgcgat tactactaaa ggagtactat
     3121 ccatttgtgc atcagcaata cctgtaattg cattcgtagc acccggaccc gatgttatta
     3181 taacaacacc cggtcttcca cttacacgag catatccttg tgctgcatgt gttgctcctt
     3241 gttcgtgacg cactaaaata tgtcttagtt tgtccttgta atcgtacaaa gcatcaaata
     3301 ccggaattat ttgtccaccg ggatacccga aaatagtatc cgtcccttcg tttaaaagtg
     3361 cttccattag ggcataagca cctgtcattg tatttgccat atctatctat gtgtaaataa
     3421 taatttcttt ttaagaacaa aattccatac aaacacgata actgttgtaa ttaactttga
     3481 tagcatgtaa tatatggaac acaagcttgt aaaaacccac attagtccta tatttagaag
     3541 aattccgctt agtcctatta ataggaaaac tgaaaactca acaatcttat tttctgttct
     3601 gtgttcatca aatatccaat aaatacttag tatgtatgta attaccaatc ctaccgaaaa
     3661 cccgcaggcg cttgatacta agtaatgaaa cccgacatat tctgtcaaaa catataaggt
     3721 tacataatca aaagcaaacg ccaatccgcc tgagcatata tatctgcata tttgcaccaa
     3781 ggtgctatga gcaggctgtt tgccaattat ttcttttata agcattatca gtttcatgca
     3841 aataatttag aatgcataag tttccatttg catagtctgt atataattga cactctgata
     3901 actccaagcc cataaattgt actacgcttt aaattaattg atgatgcatc gtcaaaatat
     3961 ttggtaggac aggttatttc tcctatttcg taacccttca taaatatttg tgccaacatc
     4021 tcgttgtcaa aaataaaatc atcagaatcg acattgtaat ttatatctct tagcacttgt
     4081 gccgaaaatg ctctataccc tgaatggtat tctgataatt tttgatgtaa caatatattt
     4141 tcaaataatg tcagaaatct gttcgaaata tatttaataa taggcatacc tccttttaat
     4201 gcgcccatac ctaaaatgcg tgatgccata acaacaggat acaaatcatt ggcaatcatg
     4261 tatgacattg actgtattaa ctttggagta tattggtaat caggatgtaa cataataact
     4321 atatcggctc ctaattccaa tgctctattg taacaggttt tctgattgcc tccataacct
     4381 ttgttctttt cgtggctaag tacttctttt atacctagtt ccttggcttt ggcaatggta
     4441 ttatctttac tgcaatcgtc agttaaaata acttcgtcaa caatgtcgaa aggaatttct
     4501 ttatatgttt gctccaatgt tttttcagca ttgtaagcag gcattacaac tacgattttt
     4561 tttccgttta gcatttcaat caatttgtag tttgcgtaca taaaaagcaa acccgttctt
     4621 tttatttatc agttttgcgt ctttaacttc tgattcgaag atgcttattt ctgatgaaat
     4681 atctttaagt acaaaataac aatctttgtc taactgccca tgcatcaaat atgatgcatc
     4741 tgcatttttg ttttttattt gtctgtcaga ataaaagtac tgtgcgtaac ttttaaaata
     4801 cggcaaatat atatagcaat cttcagcctt tttactttca taaaactcaa tggcagcagc
     4861 ctgagagtat ttctcaactt ggggaactat aaataccaat gccgaatata tgaatattat
     4921 gcatgatgca cacataatgc aaagattctt atagctaagt attttatttg atttaatgca
     4981 catccacact acgcctatta tcaaaataat actgaaagca aattcaaatc cgtaccaagt
     5041 agcacttgct tgcatgtttc ctatagcaaa ctcatctaat atgtatgggt ataataaatg
     5101 cttaaaatta tcgaattgtg ttactaaagc aactgcaaga ccaagcaagc aggctattat
     5161 tattagtaaa atctttacat actttggcat caccattttc ccatttatgt atctttcaca
     5221 tgcaaatgct gcgataaatg ttagtgggaa atagcacatt gacgagtagt gtattatttt
     5281 tgttctgact attgagaata aaattaaaac tacccaaaac agaatcatca tccatttgta
     5341 aaaacgctta aatgatgcat ctttttctgt ctttgttatc ttatagttga atgccggcaa
     5401 tgacaatatt gatgcaggaa atactcctat aagaagaatt acgaaatggt ataaagggaa
     5461 tcctccatgt cctgcgtctt ttgtttcaaa taatcgtatt tggtaaataa taaaatcttg
     5521 tattattgaa aaattgccgt caattatttg taagataaac cagaaccctc cggttaatcc
     5581 cagaataatt aaaaacaata tagcttgatg ccatttaaaa tttaatttaa aattattggt
     5641 aataagatat acgccaaaag ttaaacagaa tatcaataat ccgacaggtc ctttagtcaa
     5701 tattgctaag cctaaaaaaa aggctgatgc aacaagcttg atattaatgt ttttactatt
     5761 accggggtct gtgaaaacta taaaataata tgttccgaga aatataaaaa gattaaacca
     5821 cgggtcgata attccacttt taaaatacat aaaaggtaat agtgaacatg cgtataacat
     5881 ggtccacaat agtccgaagc gtctatctct tattttactg cctatattat ataatacaag
     5941 caatgttatg atgccgcata ttgcatcagg tatacgagca gcaaattcat taattccgaa
     6001 tagtttcatt gagactactt gcatccaaat aaataaggga ggtttttccc agaattgttc
     6061 aaaatttatt tgtactgtaa ggtaattgcc gcttgtaatc atttcgcgag cagactcggc
     6121 aaaattgatt tcatcccaat cgaatagatg aactgaacct attcctggga tgaaaaacaa
     6181 tgctgctgca atagcaatcc ctatttgaat caatatgttt tctgtctttg cgctcataaa
     6241 tagctaatca attaagcgaa cagctccttt gtcagcagaa ctaactaaag atgcgtatgc
     6301 tttcaaagat tttgatattt cgcgatttct tcctttaggt ttaaaggcat ctttaccgcg
     6361 tgcttcttct tcttttctgc gtgcggctaa ttctgcatca ctcaatttta cattaatgct
     6421 gcgagccggt atatctatct cgataatgtc tccatctttt actaaaccta tattaccgcc
     6481 tgatgcggct tccggcgaaa tgtgaccaat acttagtcct gatgtacctc ctgaaaaacg
     6541 tccgtcagtt attaatgcgc aatctttacc caagtgacga gatttgatgt aagaggtagg
     6601 gtatagcatc tcttgcatac ccggtccgcc cttaggccct tcgtgggtga taacaacaac
     6661 gtctcctgct tgaactttat ttcctaagat accttcacat gctgaatctt gcgaattaaa
     6721 aaccacagcc ttgccgctaa atttccataa actttcgtca acgcctgcag ttttaataac
     6781 acatccatcc atggcaatat tccctttcaa aatgccaagt ccaccatctt tggtgtaagc
     6841 gtgagccata tctcttatgc aaccttctgc gcggtctgta tcataactat cgtatgtggc
     6901 attttgtgaa cctaattcca aattgaattt attgcaagga gcacttgaat atatgcgttt
     6961 tgcatcgtct gaaatatttc ctgagcatat gtcatatgcc ttaataactt ctcctactgt
     7021 catgccgtca actcgttttg cttctgtatg cagcaacccg ccttttgcca attcatttaa
     7081 tattccgaga acacctcctg cgcgatttac atcttgtatg tggtattttg ttgtattagg
     7141 tgctacttta cacaaacatg gtgttttacg agatagttga tctatgtctt ccattgtaaa
     7201 attaacttct gcttcgtgtg caatagcaag caagtgtaaa actgtattgg tagaaccgcc
     7261 cattgcgatg tctaatgtca tagagtttaa aaatgcatta cgggttgcta tgcttcttgg
     7321 taggacgctt tcgtctccgt cttgatagta tgcaaatgca tttttcacaa tttgttttgc
     7381 agcatccata gctaatcttt tacgatttcc atgtgttgct aatattgtcc cattaccagg
     7441 aagcgccatt ccaagtgctt cattcaggca attcattgaa tttgctgtga acatacctga
     7501 acaacatccg caactaggac atgcgctatg ttcaatttcg tttacatctt tgtctgaaac
     7561 gcttgtatct gctgctttta ccattgcatc aataagatcc aagtgttgac ctttccattc
     7621 tccggcttcc atgggtccgc ctgatacaaa aacagcagga atatttagtc gcatagctgc
     7681 gattagcata cccggagtga ttttatcgca atttcttata caaatcatag catcagcttt
     7741 gtgtgcgtta accatgtatt caacgctatc tgcaataata tccctacttg gtaaagaata
     7801 taacatgcca tcatgtccca ttgctatacc atcgtcaatt gcgattgtgt taaattcggc
     7861 agcaaaacaa cccaaacttt cgatttcagc tttaatttct tgtcctattt cgtgtaaatg
     7921 tacgtgtccc ggaacaaatt gcgtgaatga gttaacaaca gcaataatag gtttgcctac
     7981 ttgatttggt ttcataccat tcgcacgcca caaagctctg gctgctgcca ttcttctgcc
     8041 ttgtgtacac tggtcacttc taagagagtg tttcatatct tgtcatatta agttttatag
     8101 aaaaaaaacc ttccccattt ttgcgggaaa ggttttataa tatcaattca catacccgtc
     8161 ttcatgaatg tttatacctt caagaccact aaacccacga ctacggatgt aataactttc
     8221 atttttcttc ttattagacc gcaaaagtac attttttttt tgaaaacaaa gaattaaatt
     8281 actattatat atgtaataac atgttatttc aacattctct tgttacgata tatgaatata
     8341 tttgttgtat ttattgtatt taatgatgtg tttagattta aaattgatgc aaatttactc
     8401 aaacatattt aagtaaaaga agtcaaaaaa atgcgtataa gtttatttta ttttgttgct
     8461 gcctcacgat taaattatta tctttgccaa cctaattata taaaaatcat ggaaaataat
     8521 ttactattgg atgttcgtaa gaaagctgaa atatggctta gtggagatta cgatcaagac
     8581 acaaaaactc aagtaaaagc attacttgat aacgaagaca ctaccgattt gatagaatct
     8641 ttttataaag atctagaatt cggaacaggt ggcttacgcg gtattatggg tgtcggtaca
     8701 aatcgtatga atatctatac agtaggagca gcaactcaag gcttggcaaa tttcttaaaa
     8761 aaagagtttg gcaaaggcaa acaaattaaa gttgttgtag gttatgattg tagaaataat
     8821 agccgtttgt tttctgaaac aatagcaaat attttttctg caaatggaat aaaagtttat
     8881 ttgtttgagg atctacgtcc aactccggaa atctcatttg ctattcgcga attaggttgc
     8941 caaagcggtg tggtagtaac cgccagtcat aaccccaaag agtataatgg gtataaggct
     9001 tattgggaag atggtgcgca agtaatacct ccttacgaca caagcattat ttctgaagta
     9061 aataaaataa aacacataga tgatatccat ttcaaaggta atcctgaatt gatagaaatt
     9121 ataggtgaaa atatagacaa tctattttta gaaaaaatcc aaaaattgtc tttgtcaccc
     9181 gaatcaatat ctcgtaacaa agatatgaag atcgtctata ctccaataca cggaacaggt
     9241 ataacattaa taccgaaagc attgaaaatg tatggattta acaatgtaat agatgtacct
     9301 gaacaaaatg taataagtgg cgatttccca acagtaaaat ctccaaatcc tgaagaacct
     9361 gcagctttaa aattagctgt agacaaagct cgttctgttg atgccgatat agtcatggct
     9421 tcagaccctg atgccgatcg tatggggata gcagtaaaag atgataaggg cgaatggatt
     9481 ttggttaatg gtaatcaaac agcgcaaatt ctagtatatt acttgattcg tcgttacaaa
     9541 gaattaggac gtttaaaggg taaagaatat attgttaaaa caatagtaac tacagagtta
     9601 atcgctgaaa ttgctaaacg ttataaagta ccttatttcg attgctatac aggtttcaaa
     9661 tggattgctg caattattcg tgagcaagaa ggaaagagac aatatatagg tggtggagaa
     9721 gaaagctacg gattcttggt tgaagacttt gttcgtgata aagacgcagt atcatcttgc
     9781 gttatgatgg cagaaattgc agcatgggca aaagataatg gcaagactgt ttacgagtta
     9841 ttacaagata tttatttgga attcgggttc tcgaaagaaa agggaatatc tgtagttaaa
     9901 aagggaaaaa cgggtgctga agagattaag aagatgatga ctcatttacg tagttgtcct
     9961 cctaaaacaa tagcaggttc taaagttaca attattaaag actataatgc attgcgcatg
    10021 atagatttag cagcaggatc aacaactaat atggaaatgc ctgagactgc aaatgtattg
    10081 caatttttta cagaagatgg tactaaaatt tctgttagac cttcaggaac agaaccgaaa
    10141 attaaatttt acatagaagt gagacttcca ttgaaatcgc gcgaagaatt tgatgctgtt
    10201 gatgctgctg cagaagaaaa aatattggca gctaagaaat cactgcattt agattgatat
    10261 agtaagaaaa ttaaaaaata gtcctacatc atgtgatgta ggactatttt tttgtgcata
    10321 atctgtatca gaaccagcgt ttcttaataa agaatccggt agtgaaggca gttataagta
    10381 ttgatgtaat aatgacaatc aaaaatgtcc aattaaaaat tgtagttcct gattctaagt
    10441 tcatgccgaa ataacttgct atcaacgtag gcatcattaa tacaatagat attgttgtta
    10501 agcgtttcat gacaacattc aaattgtttg atattactga tgcgtaggca tccatcattc
    10561 cacttaaaat gtcactatat atgtttgtca tttccaaagc ctgttttgat tcaatctcaa
    10621 cgtcttcaac aagttccgga tcatataagt cttttgtatt acgtaaattt ttaagcctat
    10681 gagtcagtat ctcgtttcca cgaagcgaag ttgtaaaata aacaagacac ttctctaatt
    10741 ttagcaaggc ttgtaaatct tcatttcgaa tagaattttc gagttgtttc tctgcaattt
    10801 ttgtacgatt gttaatctgt ttcaagtatt tcaaaaacca tatactcgat gacagaagca
    10861 gacgaaatac aaaatttaaa ttatctgttc gttcgatatt tttgcgtttg gtatattgta
    10921 taaaatcagt tataagatct gtttgataaa aacatacaga cacaaataca tcatccttaa
    10981 aaatgatacc taaaggtact gtagtatatg gaacagagtt aggatcttcc atttctttac
    11041 aaggtatacg cattaaaata aaacaccagc cgtcttcaac ttcgatacga ggtctttcgt
    11101 ccatatcttc aatatcttcg taaaaagcag aaggtacgtg caattcattt ataatgtaat
    11161 cggcatcgtt ttgggttggt tttacaacat taacccagca gttgggttcc cattgttggc
    11221 agggaataag tccgttcccg tttttcaaaa aggttctcat tggttttcct ccaaatgtta
    11281 aaagaatgga ggagaccaaa tccctccatt ctaaatgatt gtttgttccg gatgataatc
    11341 gtccatgttt gttttagaat ggttacggct gcaaatgtag ctataaattt tggaatgtga
    11401 aagatgcaca aataaacaat tcaattaatt aagcaatttt ttaaatgtgc gtgtgctaag
    11461 ccggaagtaa aagtattttt aaggccattt atttataaaa tatatgtatt tttctgctat
    11521 gaatataaac ataattaaag ggtacaatat tgcatttcgc aatattgtac cctttaaaat
    11581 tcattagttt agagttgatt agaccaaact ttttattaaa gtttctctat ctccgctaag
    11641 taaatccata ggagctattt cgggtagagc ataagctccg ggtttctgtt ttacgacggc
    11701 acgtgcacag gatattaaaa tttgtgctgt aagggcagga ttgttaatgc tcatagaaaa
    11761 actaatccgt tggttctggg tcttacctga aacgccttta cgttctaaaa gtacaccatg
    11821 tcccatatct tttatactat caacactttc tacttctatt acgtgtgtct cgtcgtgtgc
    11881 aaaatatgaa tctgccttaa ttgcagctgt tacttctgca attgatgctc ctttttccaa
    11941 ttctacataa accatacggc gatgtattcc tgtacctaaa ggaatagtca ttgataaggc
    12001 attctttact cctgattttg aattagcaac aacagaatgt cccatactca tacccggtcc
    12061 gaagttggta tatgtaatac ccttaggaac cattgcttga aataatgttc ttaaaacaga
    12121 gtctgtacca gggtcccatc ctgcagaaat gatagatgct actccattgg cctttgcaac
    12181 ttcatctaat tgttggtgta agtcccataa agcgccatgt atatcgtagc tgtcaactgt
    12241 gcaaatacct tttttaagaa tcgatactgc aatttcaggt actatgcgag ttggtccaca
    12301 taatatggct acatcaactt tcccaagttc gtcaatattg gaaacgacct tgataccggc
    12361 taaatcggca gtattatctg atagtgatga ggctctgcga actactccgc ataattctat
    12421 atcaggtgct gccaatgctg cttctacaga aaattttcca atatttccgt atcctataat
    12481 ggcaacttta atctttttca tattaaaaat tttaaatatt atttcttttg aatgaattca
    12541 tccatagatg ctgctgcttt gcgtccatcg cccattgcta ggatcacagt agcaccacca
    12601 cgaactatat caccacctgc aaatatcata ggaattgcgc tttgtaaatt ctcatttaca
    12661 acaatagttc ctttcttaga tacttccaat cccggtattg aatttggaat aagagggtta
    12721 ggtgatacac caacactaac aattacagta tcgacatcaa taagttct
//
LOCUS       DAOPID010000014        12547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_5942_length_12547_cov_4.752402, whole genome shotgun sequence.
ACCESSION   DAOPID010000014 DAOPID010000000
VERSION     DAOPID010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 12547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12547
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5942_length_12547_cov_4.752402"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="PK641_01165"
     CDS             <1..903
                     /locus_tag="PK641_01165"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPG54999.1"
                     /translation="LVMQQEELINYNTTFPCHGGYFYGGGRKLGCHAHSSPLNLPQSI
                     QNSCNAYYCFCLREMLDNNLAKYKTSSHAYDVWRGHVTSLGFGDKLGVDFPNEKSGLI
                     LKSSRYDKIYGKNHWRSSTIISISIGQGEVLATPIQTANLAACIANKGYFYTPHIMKS
                     IEGDSIDIKYRTKHYSTIESKYFDVVHEGMLGAVMAGTARIGRIDSLEFCGKTGTAQN
                     PHGKDHSIFIAFAPKDNPKIAIAVYVENAGFGATWAVPIASLMIEKYLRRSISPNRLY
                     LEERIMNAKFYGDTTENETESKPD"
     gene            878..2353
                     /gene="rodA"
                     /locus_tag="PK641_01170"
     CDS             878..2353
                     /gene="rodA"
                     /locus_tag="PK641_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HPG55000.1"
                     /translation="MKRKVSLIKSLDWWTIAIYIVMVIAGWVTIYAACYDFEHPEIFN
                     FSMRHGMQLIWISCAAVLSILVLLIDGKFYNNIAYLFYGLMIVVLVATIFISPDIKGS
                     HSWIKIGSFSIQPAEFAKFATALCLAKFMSSYQFNLHQARNIAIIATIICLPMLIIVG
                     QSETGSALVFAAFLLVLFREGMDGSFLMFGFIIVLFFILVLRFNNIPLEVGELPSVER
                     YGYIIVFVLAIIIGAILIKYYLNDIPFIKLYIVINIIIYFIGGIINLINASWISHTNI
                     LLMNMTASILLLITWSVYKRRKTYFMLSLFLIGTIALCYSADYAFNKILEPHQQIRIK
                     IVLGLEDDPQGAGYNVRQSQIAIGSGGFIGKGFLNGTQTKLNYVPEQDTDFIFCTIGE
                     EKGFIGTFFVVALFVILLIRLIILAERQRSPFSRIYGYSVVSILFFHFCVNIGMVIGL
                     VPVIGIPLPFFSYGGSSMWGFTILLFTFIRLDANRMEVLQQ"
     gene            2458..2703
                     /locus_tag="PK641_01175"
     CDS             2458..2703
                     /locus_tag="PK641_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPG55001.1"
                     /translation="MNLYIGNLSYKLKESDLSKVMQEYGEVVSVKIIKDRETGKSKGF
                     AFVEISDDAAGQKAMESLNGTELDGRTMVIKEANLRK"
     gene            complement(2744..3706)
                     /locus_tag="PK641_01180"
     CDS             complement(2744..3706)
                     /locus_tag="PK641_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPG55002.1"
                     /translation="MAIPAQIKLPIQDSFALYEEAYAKSLHSKDAILMPLIEHLRQSS
                     GKQMRPILVLLCAKLCGIVTQNTLHAAIALELLHVASLIHDDVVDESDKRRGYASIRA
                     KFNNKIAVLGGDFILSSALSEAASTESTAIIKVIAGLGKNLSEGELLQLETINENHSN
                     EKKYFDIISRKTAALFAACARLGFISSTNTDIETEQKIVSIGEIIGTCFQLKDDIFDY
                     EASDKIGKPTGIDLAEGKLTLPLIHVIENSSDAEKQRIKEAIENKEIIYLQDLAHQKG
                     GINYAIKKLHELQENALSILNEFPESPARTAIRAYTEYVVDRKL"
     gene            3761..4594
                     /locus_tag="PK641_01185"
     CDS             3761..4594
                     /locus_tag="PK641_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPG55003.1"
                     /translation="MKKICAITMARNDEFFLEKWINYYGNCFGEENIYILLDGEDQSI
                     PANLGKTNVIKYPRITGHIVAADKMRILILSQKAAQLLKEYDLVVGTDTDEYLVVDPK
                     CNCTLAEYLNQIDCPVCMSGLGMDVGQNLNKESEIDGGKLFLQQRSYAFVSSRYTKPV
                     IVSKPLRWGSGFHRVKGHNFHIDANLYLFHFGCFDKNILESRFSDKDRMAAGWTRHIK
                     KRFRTINFVTNKTAKNGDKYLPIARIFQTICRPIYALNKPSMACWHLVVNIPERFKNV
                     L"
     gene            4605..6011
                     /locus_tag="PK641_01190"
     CDS             4605..6011
                     /locus_tag="PK641_01190"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPG55004.1"
                     /translation="MSKYLVFIPQILVLLLLCLEFVLQLAFPSNAFYNIKQQYAFFYD
                     AAIAMFIISSSLLWISFYPPFKEFNLQTKILYAFLSIWILALNFTIHLEWQTFVLFAL
                     YAIYYGIRNKHFRIPDFMFWLAVMYFSWHLISLIWMPEFNKGLNRLLFVYLLFLVVPI
                     VFCLIEIKQNMRDMILLLFFRFMFVFVGISLCCWIAQSVYLNMPISDWFVIKKATFND
                     IAVYDYIFAWSNYYHPTYNAVAYLFALACGFYMWQKKLDISRVNTIELAIYTCMSLVL
                     VLITQSRIGIITWFLVFVLGIAYLLRRHKKLIIFGTSLSFIMLVIAMYFASPFIESFF
                     KDTARVQNFGTAFAYIKTNYLLGTGVEGIRAIMDSEEFAHSLGYEFAYVGLANPHHQF
                     IGDLMQTGIVGVLLSLAMTIYLFVISIKRHNYPLLVFMVIFFMIMQIEMPFYLPKGTM
                     FFLAFVFLLCPQSASNSR"
     gene            complement(5987..7066)
                     /locus_tag="PK641_01195"
     CDS             complement(5987..7066)
                     /locus_tag="PK641_01195"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPG55005.1"
                     /translation="MAKINKKRILIDLYMIKHPNCGFGQIAINYAKYFLNEYHAGEDF
                     DIYLLVPKQYIGAFGTEVKYINAVWWKKIFKKCIPFMDIWHSTNQIPRFMPYAKKTKY
                     LLTLHDFNHEYEKKGAALRHSRRHWAALIKRSDLIVCISQFTAAETKKYANIEGKPNP
                     MVILNGVEQFDNQIASKPTSIVSDKPFFFSIGEMRAKKNFHVLLDMMKLMPDKQLYLA
                     GSPTTEYAALLSKRIQDEHIDNVKMLGRISDEERIWLYANCEAFVFPSLFEGFGLPII
                     EAMQFGKPVFSSTFTSLKEIGGEYAFFWQNFDAKTMSETVRNGLETFKNDNTLAEKEK
                     EYAAGFSYQKNIESYLKLYRELLAD"
     gene            complement(7053..8066)
                     /locus_tag="PK641_01200"
     CDS             complement(7053..8066)
                     /locus_tag="PK641_01200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPG55006.1"
                     /translation="MARILVIRFASLGDIAISIPPVLAFAEQYPNHELFYLSNKRFAS
                     LFETMPDNVHFIGADLKGEHSSLYGLYKLAKEFRKLKIDYVADFHNVIKSQFICSILR
                     LSGIKIASRKKDKISRLKLLHRHGKAIYDLKSYHERYADVFADLSLPIKLKFQKLNIP
                     SPIIKPENEKWIGIAPFSAHKGKIYPIEKIEEIVAYYSSKANIKLFLFGGGKYESSVF
                     EKWIDTYKTPFSMVGKYELQEELGVMGKLDVMVSMDSANMHLAALMNTTVISIWGPSH
                     PAIGFRPWNQNKDTFIQIDLPCRPCSEWGAKPCKSGDFACMQGIKVQTITNKIDSLIY
                     GKN"
     gene            complement(8069..8674)
                     /locus_tag="PK641_01205"
     CDS             complement(8069..8674)
                     /locus_tag="PK641_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007756356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4254 domain-containing protein"
                     /protein_id="HPG55007.1"
                     /translation="MKFTSLCTEIFSQSINNYHKTDNVDASIYNPYPSKSIEYYLYLK
                     NWIDTVQWHLEDIIRDPNIEAREALAIKRRIDKSNQDRTDLVELIDSYFLDLYKDVKH
                     LDSATINTESPAWAIDRLSILALKIYHMKQETLRSDSSADHKKQCECKLAILNQQNID
                     LQTAIDQLLEDIASGRKFMKVYKQMKMYNDPELNPTLYGNK"
     gene            complement(8688..9242)
                     /locus_tag="PK641_01210"
     CDS             complement(8688..9242)
                     /locus_tag="PK641_01210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HPG55008.1"
                     /translation="MNICVFCSSSDKIADSYAESAEQLGKWIGLNSHTLVYGGASKGL
                     MEVCAKSVFENGGDVISIIPHFVDSRGIVSQYAGQVFRVDNLAQRKEMMQEYADAFVV
                     LPGGIGTLDELFSALACCVVGEISKPIIILNQNGIYENLQQQIIRMTQEGFIPKEPQF
                     EAQFVENVELCIKALSFIDSENQQ"
     gene            complement(9249..11213)
                     /gene="gyrB"
                     /locus_tag="PK641_01215"
     CDS             complement(9249..11213)
                     /gene="gyrB"
                     /locus_tag="PK641_01215"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HPG55009.1"
                     /translation="MSEEEKTATNSYEASSITVLEGLEAVRMRPAMYIGDISVKGLHH
                     LVYEVVDNSIDEALAGYCTHIEVTINKDNSITVRDDGRGIPVDMHKKEGKSALEVVMT
                     VLHAGGKFDKGSYKVSGGLHGVGVSCVNALSTSLRAEVRRNGKLYMQEYRCGKPVEPV
                     KVIGTSEETGTMVTFLPDSSIFITTEYHYDILANRLRELSYLNAGIRLSLTDNRVVNE
                     DGSFKNDVFYSKEGLKEFVLFVDEAREKLFNEVIHISTDKYETPVDIAMIYNTTYNEN
                     IHSYVNNINTIEGGTHLAGFRRGLTRTLKKYAEDNKLLDKLEKQKIELDGNDFREGLT
                     AVISIKVAEPQFEGQTKTKLGNNEVMGSVDMAVSEALGNYLEEHPREAKLIVDKVILA
                     ATARHAARKARELVQRKSPLAGGGLPGKLADCSSKDPSECEIFLVEGDSAGGTAKQGR
                     DRRTQAILPLRGKILNVEKAMQHKAFESEEIRNIYTALGVTIGTEEDSKALNMNKLRY
                     NKVIIMTDADIDGSHIATLILTFFFRYMKELIEGGHVYLATPPLYLCKKGKIEEYCWT
                     EQQRRTFIETYGGGSESAVYVQRYKGLGEMSDEQLKMTTMSKEHRTLRQITIENAVAA
                     DHVFSMLMGDDVAPRREFIEKNATYANIDA"
     gene            11434..12417
                     /locus_tag="PK641_01220"
     CDS             11434..12417
                     /locus_tag="PK641_01220"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HPG55010.1"
                     /translation="MKPQIAIVAGGDSSEFGVSLRSAQGIKSFIDADRYETTVVTITK
                     KAWQAELSETEKYPIDKNDFSYVSSDGKKHNFDFAYITIHGTPGENGILQGYFDLLGL
                     KYSCCGVLAASMTFNKFACNRYLQAFGAKTAKSILIRKGNEFDAKNIISQLGLPCFVK
                     SNVGGSSFGVTKVKAEETIIPAIDMAFQEGDEVIVESFLKGTELTCGMYKTKSKTVVF
                     PLTEVVTSNEFFDYDAKYNGQVQEITPARLSPELTAEIQAQTAKYYDILGCKGIVRID
                     YIVSEDGVPYVLEANTTPGMTATSFVPQQVRAAGLDIKDVMTDIIENEIGR"
BASE COUNT         3735 a         2119 c         2318 g         4375 t
ORIGIN      
        1 cttgtaatgc aacaggagga acttatcaat tataatacaa ctttcccttg tcatggaggt
       61 tatttttacg gaggagggcg taaattaggt tgtcacgcac attcttctcc attgaatttg
      121 ccgcaatcaa ttcaaaactc gtgtaatgca tattattgtt tttgcttgag agagatgtta
      181 gataataatc ttgctaagta caaaacatca agccatgcgt atgatgtttg gcgtggtcat
      241 gtaacttctc taggttttgg cgacaaatta ggtgttgatt ttcctaacga aaaaagcgga
      301 ttaattctta agagttcgcg ttatgataag atatatggta agaaccattg gaggtcttct
      361 actataattt caatctcaat tggtcaaggt gaggtattgg caactcctat tcaaacagca
      421 aatttagcag cttgtatagc taataaaggt tatttctata cacctcatat aatgaagtct
      481 attgagggtg atagcattga tattaaatat agaactaagc attattcaac tatagaatcg
      541 aagtactttg atgttgtgca tgaaggtatg ctaggtgctg taatggcagg tactgcacgt
      601 atcggacgaa ttgatagttt ggaattttgc ggtaaaacag gaactgcaca gaatccacac
      661 ggaaaagatc actccatttt tattgctttt gcgcctaaag acaatcctaa aatagcgatt
      721 gccgtatatg ttgaaaatgc cggttttggt gcaacatggg ctgttcctat tgcaagctta
      781 atgattgaaa aatatttacg tcgttcaatt agtccaaatc gtttatattt ggaagaaaga
      841 ataatgaacg caaaatttta cggagataca acggaaaatg aaacggaaag taagcctgat
      901 taaatcattg gattggtgga ctatagcaat ttatattgtt atggttattg ccggatgggt
      961 aacaatatac gccgcatgtt atgattttga acatccggaa atatttaatt tctcgatgcg
     1021 tcatggtatg caattgatat ggatctcttg tgccgcagtt ttatcgattt tagtattact
     1081 tattgatggt aaattttata acaatatagc ttatctattt tatgggctga tgatagtggt
     1141 actagttgca acaattttta tatcgccaga cattaaaggc tcacattcat ggattaaaat
     1201 aggatctttc agtatacagc cggcggaatt tgcaaaattc gcaactgctt tgtgtttggc
     1261 aaaattcatg agttcttatc agtttaatct gcaccaagct cgtaatattg ctattattgc
     1321 aacaataata tgcttgccta tgttgattat tgttgggcag agtgaaaccg gttctgcatt
     1381 ggtttttgcg gctttcttat tagtgttatt ccgcgaaggt atggatggct catttcttat
     1441 gtttgggttc attattgttt tgttttttat attagtttta aggtttaaca atattccttt
     1501 agaggttggt gagctgccat cagtcgaaag atatggttat ataattgtgt ttgtacttgc
     1561 aataattata ggcgcaattc ttataaaata ctatttgaat gatataccat ttatcaaatt
     1621 atatatagtt ataaatatta taatctattt tataggtgga attataaatt taataaatgc
     1681 atcgtggatt tcgcatacga acatattgct aatgaacatg actgcaagta tattgttatt
     1741 gattacatgg agcgtataca agagacgaaa aacatatttc atgctttctt tgtttttgat
     1801 tggtacaatt gctctttgtt attctgcaga ctatgctttt aacaagattt tggagcctca
     1861 tcaacaaata cgtataaaaa ttgttttagg tttagaagat gatccgcaag gagcaggtta
     1921 taatgtacga caatcgcaga tcgcaatagg ttcgggtgga tttataggca aaggtttctt
     1981 aaatggcact caaacaaaac tgaattatgt accggaacaa gacactgact ttattttttg
     2041 tactataggt gaggaaaaag gttttatagg tacctttttc gttgttgctt tgtttgttat
     2101 actgttgatt cggcttatca ttctggcaga gcgacaaaga tcgccattca gtagaattta
     2161 cggctatagc gtagttagta ttttgttctt ccatttttgc gtcaatatag ggatggtaat
     2221 aggcttggtt cctgttatcg gtattccgct tccatttttt agttatggag gctcttctat
     2281 gtggggcttt acgatattgc tatttacttt tattcgctta gatgctaaca gaatggaagt
     2341 cttacaacaa taatttcccc ttgaaaaaat tgtaaatcca aaatttatga ctttctttgc
     2401 gctgcgattt tgaatttcaa aattgcgaaa catttattta tttatctatt tttctttatg
     2461 aacctttaca ttggaaacct tagctataag ctaaaggaaa gtgacttatc aaaagttatg
     2521 caagagtatg gtgaagtagt ttcagtaaag attatcaaag atcgcgaaac cggtaaatca
     2581 aaaggttttg ctttcgttga aatctcagac gatgctgctg gccaaaaagc catggaatca
     2641 ttaaatggca cagaattaga cggacgcacg atggtgataa aagaagccaa cttacgtaaa
     2701 tgagcttaat cttgggcgga tcttagggtc cgcctttttt tatttataat tttctgtcaa
     2761 ctacgtattc ggtataagcc ctaattgcag ttcttgcagg gctttccggg aattcattta
     2821 atatagaaag agcattttct tgtagttcgt gcaacttttt tattgcgtaa ttgattcctc
     2881 ctttttggtg agctaagtct tgtagataaa ttatttcttt attttcaata gcttctttaa
     2941 tgcgttgctt ttctgcatca gaagaattct ctattacatg aatcaaagga agggtaagct
     3001 taccctcggc aagatcaata ccggttggtt tgccaatttt atcgctagct tcgtaatcaa
     3061 aaatatcatc ttttaattgg aagcatgttc caataatctc gccaatagat acgatcttct
     3121 gttctgtctc aatatcagta tttgtagatg aaataaaacc aagtcttgcg catgctgcaa
     3181 aaagagctgc tgtctttcgg ctaattatat caaaatactt tttttcgttc gagtgatttt
     3241 catttatggt ttctaattgt aataattcac cttcacttag atttttaccc agtccggcaa
     3301 taaccttaat aattgctgta ctttcggtgg atgcagcttc cgatagggca gatgaaagaa
     3361 tgaagtcacc tcctaaaact gcaattttgt tattgaattt ggcgcgtatc gaagcgtagc
     3421 ctctacgttt atcagactca tcaacaacat catcgtgtat taagctggca acatgaagta
     3481 actctaatgc aattgctgca tgtaaggtgt tttgtgtaac aataccacat aactttgcgc
     3541 ataacaaaac caaaatagga cgcatctgtt taccgcttga ttggcgtaag tgttctatta
     3601 aaggcataag gattgcatct tttgaatgta aagattttgc gtaggcctct tcgtataatg
     3661 caaaggagtc ttgtatagga agttttattt gcgctggtat cgccatacga ttcttaaata
     3721 gaatttgcaa aagtagtaaa aaaatcctta cttttgtgtc atgaaaaaaa tatgcgccat
     3781 aacaatggcg agaaatgatg aattttttct tgaaaaatgg ataaattact acggaaattg
     3841 tttcggagaa gagaatatat acattttact cgacggagaa gatcaatcta ttcctgctaa
     3901 tttgggaaaa acaaatgtta taaaatatcc aagaattacc ggacatattg ttgctgcaga
     3961 taagatgcga atacttatat tatcgcaaaa ggctgctcag ttattaaaag aatatgattt
     4021 agtagttggc acagatacag atgagtattt agtggtagat cctaaatgta attgtacatt
     4081 agcagaatac ctaaatcaaa ttgattgccc cgtttgtatg tcaggtttag gaatggatgt
     4141 aggtcaaaat ttgaataaag aatcagagat agacggaggc aaattatttt tacagcaaag
     4201 aagttacgca tttgtgtcgt cgcgatatac taaaccggtt atagtttcca aaccgctacg
     4261 ttggggatca ggttttcatc gtgttaaagg gcataatttt catatagatg cgaatctcta
     4321 tttatttcat tttggatgtt ttgataaaaa catacttgaa agtagatttt cggataaaga
     4381 cagaatggcg gcaggctgga ctaggcatat aaaaaagcgt tttagaacga ttaattttgt
     4441 aacaaacaaa acagccaaaa atggtgataa atatttaccc atagcccgaa tatttcaaac
     4501 aatttgtcgt cctatatatg cactaaataa accatcaatg gcttgttggc atttggttgt
     4561 aaatatacct gagagattta agaatgtgtt gtaataaaac tactatgagt aaatacttag
     4621 tatttattcc tcaaatcctt gttttattgt tgctatgctt ggaattcgta ctacaattag
     4681 cattcccaag caacgcgttt tataatatta agcagcaata tgcttttttt tatgatgcag
     4741 cgatagccat gttcattata tcgtcatcat tattatggat ttccttttat cctccattca
     4801 aagagtttaa tcttcagact aagatcttat acgcattttt atcaatatgg attctcgctc
     4861 taaattttac aatacactta gagtggcaga catttgtttt attcgcttta tatgcgattt
     4921 attatggcat caggaataag cattttcgga ttcctgattt tatgttttgg cttgctgtaa
     4981 tgtatttttc ttggcattta attagtttaa tatggatgcc tgaatttaac aaaggtctta
     5041 atagattact atttgtgtat ttgttgtttt tagttgtacc aatagtattt tgtttaatag
     5101 aaataaaaca aaatatgcga gacatgattt tgcttttatt ttttaggttt atgttcgtat
     5161 ttgtaggtat atcattatgt tgctggatag ctcaaagtgt atatttaaat atgccgataa
     5221 gcgattggtt tgtaattaaa aaagcgacgt ttaatgatat tgccgtttac gattacatat
     5281 ttgcgtggtc taattattat catcctacat ataatgcggt agcctattta tttgctttgg
     5341 cttgtggatt ttatatgtgg caaaaaaagc tggatatttc gcgtgttaac actatagaat
     5401 tagcaatata tacttgtatg tcactggttt tggtgttaat aacccaatct cgcataggta
     5461 ttattacttg gtttttagtg tttgttttag gtattgcata tcttttaagg cgtcacaaaa
     5521 agctgataat attcgggacc tccttgtctt ttattatgct tgtaatagct atgtattttg
     5581 ctagtccttt tattgaatca ttttttaaag ataccgctcg tgtgcagaat tttggcacag
     5641 cgttcgcata cataaaaact aattaccttt tgggaacagg cgttgaggga ataagagcta
     5701 taatggattc ggaagaattc gcacattcat taggctatga atttgcatat gtaggattgg
     5761 caaatcctca tcatcaattt ataggagatt tgatgcagac cggtatagtc ggagtgttac
     5821 tatctttagc tatgactatt tatctgtttg ttatatccat aaaacgtcat aactatccat
     5881 tactggtttt tatggtgata ttttttatga taatgcaaat agagatgccc ttttatttgc
     5941 ccaaaggaac aatgtttttt ttggcttttg tctttctgtt gtgtcctcaa tcagcaagca
     6001 attctcgata gagctttaag tagctttcaa tattcttttg ataagaaaaa ccggcagcat
     6061 attctttctc tttttctgct agagtattgt cgtttttaaa tgtttcaaga ccattcctaa
     6121 cagtttcaga cattgttttt gcatcaaaat tctgccaaaa gaaagcatat tctccaccaa
     6181 tttcttttaa acttgtaaag gtagatgaaa atacaggctt cccaaattgc atagcctcaa
     6241 taatgggtaa tccaaagcct tcaaacaaag aaggaaatac gaatgcttca caatttgcat
     6301 atagccaaat acgttcctca tcacttattc tacccaacat ttttacattg tcaatatgct
     6361 catcttgaat gcgtttgctt aataatgcag catattctgt agtaggagaa cctgcaagat
     6421 ataattgctt gtcaggcatc aatttcatca tatcaagtaa tacgtgaaag tttttcttag
     6481 ccctcatttc acctatgctg aagaaaaacg gcttatcaga aacgatgctt gttggtttgc
     6541 tagcaatttg gttgtcaaat tgctcaactc cgtttagaat taccataggg tttggtttac
     6601 cttcgatatt agcgtatttc tttgtttctg ctgcggtaaa ctgcgatata catacaatta
     6661 ggtccgaacg tttaattagt gcagcccagt gtctcctact atgccgcaat gctgcacctt
     6721 ttttttcgta ttcgtggtta aaatcgtgca gtgtgagcaa atattttgtt tttttggcgt
     6781 atggcataaa tcttggtatt tgatttgtgg aatgccaaat gtccataaat gggatacatt
     6841 tcttaaaaat ttttttccac catactgcat ttatatattt aacttctgta ccaaaagctc
     6901 ctatatattg ttttggaact aacagataaa tatcaaagtc ttcgccggca tggtattcgt
     6961 taagaaagta ttttgcataa ttaatagcaa tctgaccaaa tccgcaattc gggtgtttga
     7021 tcatgtaaag gtcaataagt atacgtttct tattaatttt tgccatatat cagactatca
     7081 attttatttg taatagtttg aacttttata ccttgcatac aagcaaaatc accacttttg
     7141 catggttttg caccccattc tgagcatggt ctgcatggca ggtctatttg tataaaggtg
     7201 tctttattct gattccacgg tctgaatcct atagcaggat gcgatgggcc ccatatagag
     7261 attacagttg tattcattaa tgctgcgaga tgcatatttg cagaatccat agagaccatt
     7321 acatctaatt ttcccataac tccaagctct tcttgtagtt cgtatttgcc taccattgaa
     7381 aaaggagttt tatatgtatc aatccatttc tcaaatacag aagattcata cttaccacct
     7441 ccaaacaaga aaagttttat attggccttt gatgaatagt atgcaacaat ttcttctatt
     7501 ttttctattg gatatatttt gcctttgtgt gcagagaatg gagctatacc tatccacttt
     7561 tcgttttcag gcttaattat tggtgatggt atattaagtt tttggaactt cagttttata
     7621 gggagagata aatctgcgaa aacatctgca tatcgttcgt gatacgattt taaatcatag
     7681 atggcttttc catgtctgtg caatagtttt aatctgctta ttttatcttt cttgcgtgat
     7741 gcaattttaa ttccggataa tctcaatata ctgcagataa attggctttt tataacatta
     7801 tgaaaatcag caacatagtc aatcttcagt ttcctaaatt ctttagcgag tttatataat
     7861 ccatataaag aagagtgttc tccttttaaa tcggcaccaa taaagtgtac gttatcaggc
     7921 atagtttcaa atagagatgc gaatcgctta ttgctcaaat aaaataactc gtgatttgga
     7981 tattgctctg caaaagctaa taccggaggt attgatatag cgatatctcc caaggatgca
     8041 aaccgaatta caaggattcg tgccatttct atttattgcc gtatagtgta ggattcaatt
     8101 cagggtcgtt atacattttc atttgtttgt atactttcat gaattttcgt cctgatgcga
     8161 tatcttctaa taattggtca atagccgttt gtagatctat gttttgctga tttagtatgg
     8221 ctaacttgca ttcgcactgc tttttatgat ctgcagatga gtcggaacgc aaggtttctt
     8281 gtttcatgtg atatattttg agagctaaga tcgacaatcg atcgattgcc catgccggac
     8341 tttctgtgtt gatagtagca ctatctaagt gttttacatc tttgtataag tctagaaaat
     8401 agctatcaat caattctacc aggtctgttc tatcttgatt tgacttgtca atgcggcgtt
     8461 taatggctaa ggcttcacga gcttcgatat tcggatccct tattatatct tctaaatgcc
     8521 attgaactgt atcaatccaa ttttttaaat aaagataata ttctatggat ttgctaggat
     8581 aaggattgta tatactagca tcaacattat cggttttatg gtagttatta atagactgac
     8641 taaatatttc ggtgcataat gatgtaaact tcatttcgtc aattttttta ttgttggttt
     8701 tctgaatcaa tgaatgataa tgcttttatg caaagttcaa cattttccac aaattgagct
     8761 tcaaattgag gctcttttgg gataaagcct tcttgggtca tgcgaattat ttgctgttgc
     8821 agattctcgt agatgccatt ttgattcaaa atgattatgg gttttgaaat ctctccaact
     8881 acacaacagg ctaatgcact gaataactcg tctaaagtgc ctatgccacc gggaagaact
     8941 acaaatgcat cagcatattc ctgcatcatt tctttgcgtt gggcaaggtt gtcaactctg
     9001 aaaacctgtc ctgcatattg actaacaata cctctgctat caacaaaatg cgggattata
     9061 cttataacgt ctcccccatt ttcgaataca gatttggcac agacttccat taaaccttta
     9121 gaggcacctc cgtataccaa tgtgtgacta ttaagcccaa tccatttgcc tagttgttcc
     9181 gcagactctg cgtaagaatc ggcaatttta tcacttgatg agcaaaaaac gcaaatattc
     9241 atatcctatc aagcatcaat attagcgtat gtagcattct tttcgataaa ttcgcgacgt
     9301 ggagcaacat catcacccat cagcattgaa aatacatgat cggcagcaac tgcattctca
     9361 attgtaattt gacgtaatgt tctatgttct ttgctcatgg ttgtcatctt caattgctcg
     9421 tcgctcattt cacccaaacc tttgtatcgt tgtacatata ctgcagattc gctgccgcca
     9481 ccataagttt cgataaatgt acggcgttgt tgttctgtcc agcagtattc ttctattttg
     9541 ccttttttac aaaggtaaag tggaggagtt gccagatata cgtgtccgcc ttctattaat
     9601 tctttcatat agcggaagaa aaaggtaaga ataagagtgg caatgtggct accatcaata
     9661 tcagcatcag tcatgatgat aactttgttg taacgaagtt tattcatgtt caatgccttt
     9721 gaatcttctt ctgtaccaat tgttacacct aaagctgtgt atatgttacg aatttcttcg
     9781 ctctcaaatg ctttgtgttg catagccttc tcaacattaa gaatcttacc acgtaaaggc
     9841 aaaatagctt gtgttctacg gtcacggcct tgttttgccg taccacctgc tgaatctccc
     9901 tctacaagga atatttcaca ctcagaagga tctttgcttg agcagtcagc taatttaccg
     9961 ggtaaaccgc ctcctgctaa tggagatttg cgctgtacaa gttcacgtgc tttacgtgct
    10021 gcatggcgtg ctgtagctgc taaaataact ttgtcgacaa taagtttagc ttcgcgaggg
    10081 tgctcttcaa gataatttcc aagagcctca cttacagcca tatcaacaga acccattact
    10141 tcgttgtttc ccaactttgt ttttgtttga ccctcaaatt gtggttctgc aactttaatt
    10201 gagataacgg cagttaaacc ttcgcggaag tcgttaccat caagttcgat tttttgtttt
    10261 tctaacttat caagtaattt attgtcttct gcatattttt tcaaggtacg agtaagtccg
    10321 cggcgaaatc ctgcaaggtg cgttcctcct tctatcgtat tgatgttgtt aacgtaagag
    10381 tggatatttt cgttgtatgt tgtattatat atcattgcga tatccacagg agtctcatat
    10441 ttatctgttg atatatgaat tacttcgtta aataatttct cgcgtgcttc atctacaaac
    10501 aagacaaatt cttttaatcc ttcttttgaa taaaagacat catttttaaa tgaaccatct
    10561 tcgtttacaa cacgattgtc tgtaagagaa aggcgaatac ctgcatttaa atatgataat
    10621 tcgcgtaagc ggttagcaag aatgtcgtag tgatattctg tagttatgaa gatgcttgag
    10681 tcaggaagaa aggttaccat tgtacctgtt tcttctgaag tgccgatgac ctttaccggt
    10741 tccacaggtt taccgcagcg atactcttgc atgtatagtt ttccattcct gcgaacttca
    10801 gcgcgaagtg aagttgatag tgcatttacg cacgatacac ctacaccatg taaacctccg
    10861 gatactttgt agctgccttt atcgaatttg ccacccgcgt gcaatacagt cataacaacc
    10921 tctaatgctg atttaccctc ttttttatgc atatcaacag ggataccacg accgtcgtct
    10981 cttactgtta ttgagttgtc tttgttgatg gttacttcta tatgtgtgca gtaaccggca
    11041 agagcttcat caatggagtt atctacgacc tcataaacta aatgatgcag accctttaca
    11101 ctaatatcac ctatgtacat ggccggacgc atacgaacag cttccaaacc ttctagaacg
    11161 gtaatactac tggcttcata gctatttgta gccgtctttt cttcttcact cataacttat
    11221 ctgattgtaa taaaaattag tattaatttc aatgctcaat aaacgtacaa atttacaaaa
    11281 aaagtatgag aggacgaaac ttttaggatt ctaattaatc tacaaataac aaaaaatatg
    11341 acaaaatatg cctattgtat tgaaaaaatt gtagtatttt tgaaactttg tttgactcaa
    11401 gattgttaac aatcaattta gtataaaatt gttatgaaac cacagattgc aattgtagcc
    11461 ggaggtgact cttcagagtt tggtgtgtct ttaagaagtg cacaaggaat caaatctttt
    11521 attgatgcag ataggtacga aacgacagtt gtaactatta caaaaaaggc atggcaagca
    11581 gaattatctg aaacagaaaa atatccgatt gacaaaaatg acttttctta tgtgtcgtct
    11641 gacggcaaga aacataattt tgattttgcg tacataacaa ttcacggaac acccggcgaa
    11701 aacggaattt tgcaaggata ttttgatttg ttgggtttaa aatactcttg ttgtggtgta
    11761 ttagcagcct caatgacttt caataaattt gcatgtaaca gatacttgca agcctttggt
    11821 gctaaaactg caaaatctat tttgatacgt aaagggaatg agtttgatgc aaaaaatatt
    11881 atatctcaat taggcttacc ttgtttcgta aaatcaaatg ttggaggtag tagcttcgga
    11941 gttactaaag taaaggccga agaaacaatt ataccggcta ttgatatggc ttttcaagag
    12001 ggtgatgagg ttattgttga atctttctta aaaggcacgg agctaacttg cggtatgtat
    12061 aaaacaaaaa gcaaaacagt tgttttccct ttgacagagg ttgtaacatc aaatgagttt
    12121 tttgattatg atgctaaata caatggtcaa gtgcaagaaa ttactcctgc gcgcttgtct
    12181 cccgaactga cagccgaaat ccaagctcaa acagcaaaat attacgatat attgggatgc
    12241 aaaggcattg tacgcattga ctatatagtt agtgaagacg gtgttccgta tgtcctggaa
    12301 gcaaacacga caccaggaat gactgctaca agttttgtcc cacagcaagt tcgtgctgcc
    12361 ggtttggata ttaaagacgt aatgacggat attattgaaa acgaaatagg gaggtagata
    12421 tgcaaaaaac acaagacgaa ttgtttgctt tggttgagca agccatagtt agtatgaatt
    12481 ggaagaaaga accgatcggt ttgtacgatc ccatagaata tgttctttct ttaagcggaa
    12541 agcgcct
//
LOCUS       DAOPID010000015        12376 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_6072_length_12376_cov_4.743203, whole genome shotgun sequence.
ACCESSION   DAOPID010000015 DAOPID010000000
VERSION     DAOPID010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 12376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12376
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6072_length_12376_cov_4.743203"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="PK641_01225"
     CDS             <1..566
                     /locus_tag="PK641_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HPG55011.1"
                     /translation="KVVTMVYGGLVNKNIVAGLQKLGINALGLTGADMAVIKSDKRPV
                     KDIDYGYVGDVKSVDGKLLANLIEQGVVPIVAPLSFSSEYGLLNTNADTIAAETAKAL
                     SQFFDVTLTYCFEKKGVLMDENDDESVIRTITPDLFLEYKNDNIIQGGMIPKLENAFD
                     ALKAGVSKVVITKASELGKESGTSVLA"
     gene            586..1632
                     /locus_tag="PK641_01230"
     CDS             586..1632
                     /locus_tag="PK641_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HPG55012.1"
                     /translation="MIDIAALLKRLIEIPSTSMNESAKADFLYNELKNKDCEPNRFKD
                     NLWCMAYPYDAAKHTIMLNSHIDTVKPNASWTYEPNKATEVDGRIYGLGSNDANASVV
                     ALIETFLRLKQTKQSYNLILALSAQEEVSGKDGIECLLNELPKIDFAIVGEPTGMRLA
                     IAEKGLMVLDCVAHGISGHAARDEGDNAIYKAMKDVEWFRTFEFPKISDTLGKVKMSV
                     TIVNAGTQHNVVPDTCSFTVDVRLTDCYSHEDVLSIVRKSVSCDVTPRSMRLRPSGVP
                     LSNFFVKRYLATGGSVFGSSTMSDQALMPFASVKIGPGDSARSHTADEYIELSEIFKA
                     VDIYYNLLDGLIIM"
     gene            1830..2057
                     /locus_tag="PK641_01235"
     CDS             1830..2057
                     /locus_tag="PK641_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55013.1"
                     /translation="MLFSACSSNEPLIDDVTEKCWEYNYSAFGYSVKDYYWATAKEFG
                     EYKTTLESMYGTVTSYKAVSKSESECESSEY"
     gene            complement(2110..2568)
                     /locus_tag="PK641_01240"
     CDS             complement(2110..2568)
                     /locus_tag="PK641_01240"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HPG55014.1"
                     /translation="MGIEIEKKFLVNKKKWNKPNTGCLYTQAYLAKDGCTLRVRIAET
                     KAFITIKGPTVGCSRKEFEYEIPIADAQDMMKLALYQPIVKIRYKQEIGGKIWEIDEF
                     LDCNEGLIVAEIELNSEQESFIVPEWVDKEVTSDKRYTNASLSKHPYKAW"
     gene            2646..3788
                     /locus_tag="PK641_01245"
     CDS             2646..3788
                     /locus_tag="PK641_01245"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPG55015.1"
                     /translation="MRVPSQYDDIRRYYDEEVNPALAEILAQTQISKILAAILGGDNA
                     EYFLRTLAPINSIDDFQREVIVVLLTAIEHKTCTEVKLYGTEYIDRSKGHLFMSNHRD
                     IVLDSAFLNVHLFYKGFKTTQIAIGNNLLIYPWIEHAVRINKSFVVRRDGSIKEQLLI
                     SRKLSEYMRIVLSEKKESIWLAQREGRAKDSNDITQSSVIKMLNMSGRKSFSENMREL
                     NITPVSINYEYDPCDFLKAKEFQQKRDDANFKKSPGDDIKSMQTGIMGRKGRVSLVIS
                     EPLNIDEAWNKLPKNEQAEALSKDLDTSIHRNYLLFENNYIAADLLSGGTDFSGHYSA
                     SQVATFVRYIENQLLKIDLPNRDEMFLRRKMYEMYANPAINQSKLL"
     gene            3790..4683
                     /gene="xerD"
                     /locus_tag="PK641_01250"
     CDS             3790..4683
                     /gene="xerD"
                     /locus_tag="PK641_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HPG55016.1"
                     /translation="MSAISEQYRRYLLLERAFSPNTMDAYMHDVDKLLDYLDAKNLTY
                     LTASTDDLSRFLMTLTDLGIAYRSQARIVSGIKSFYKFLIMEDLIKENPADLLELPKL
                     AKKLPIVLSIEEIDRIESVIDLSKQEGQRNKAIIETLYSCGLRVSELVNLKLSSIYWD
                     EEYLKVEGKGSKQRLVPISKTALKEIRNYLPDRNALTITKGNGDYVFLNRRGAKLTRQ
                     MVFMWLKRYAGEAGIKTVISPHTLRHSFATHLLEGGANLRVIQEMLGHESILTTEIYT
                     HITMDYLRKTIMDCHPRSSHI"
     gene            4773..5774
                     /gene="pta"
                     /locus_tag="PK641_01255"
     CDS             4773..5774
                     /gene="pta"
                     /locus_tag="PK641_01255"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009433101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HPG55017.1"
                     /translation="MNIFDKLTANAKSELKRIVLPESLEERTIKAADIALAEGLADIV
                     LLGEKKTILAKANELGLSNIERATIFDVNTNPKAAEYVGLLYELRKEKGMTEDEAKKL
                     VYGNPLYLASLMIKNGDADGEVAGAMNATSNVLRPALQIIKMKKGVNVVSGAFLMIVP
                     NESYGEKGMFLFADCAVTPDPTAQQLAEIAVVSASTAKVFGEFDPRIAMLSFSTKGSA
                     KHESVEKVQEATRLAKEMSPNLLLDGEFQADAAIVPSIGKSKAPNSPIQGNANVLIFP
                     NLDAGNIGYKLVQRLSGGIAIGPILQGMASPVNDLSRGCSVDDIVKMIVITSNQTRL"
     gene            5782..6975
                     /locus_tag="PK641_01260"
     CDS             5782..6975
                     /locus_tag="PK641_01260"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HPG55018.1"
                     /translation="MKILVLNCGSSSVKFKLFDMDKKIVLTQGGIEKLGMKDSFLKMT
                     LPNGEKKVFNDFMLDHHVAIALILKILVSKEYGAIKSLDEIDAVGHRVVHGGENFNKS
                     VIITKEVIDGVKACIDMAPLHNPANLEGIYTISKILPKVPQVAVFDTAFHQTMPEYAY
                     MYGIPYELYQKYKVRRYGFHGTSHRYVSKHVCEFLGVEQEGKRIITCHIGNGGSITAI
                     KDGKSIDTSMGFTPLEGLLMGTRSGNVDPGALDFLVEKEQLTSVSLSNLLNKKSGVLG
                     VSGVSSDMRDLDAAAKDGNHRAELALKMYAYRILKYIGAYTAVLGGLDILVFTGGVGE
                     NQIEIRAKICSGLKYLGIEIDDNRNQLRGQDAIISSDSSRVKVAVIPTDEEFMIAIDT
                     MELVH"
     gene            6975..7601
                     /locus_tag="PK641_01265"
     CDS             6975..7601
                     /locus_tag="PK641_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55019.1"
                     /translation="MREKIAKILTLLFQPIFVPLYGFILLWNSSYFYPYSIFIKVMVL
                     GLSSLFMVILPAIFYAILYKRGKITTPQASNSKERIPVYMFTMCSYSMLIYLLLYIGV
                     VYYFTYIALGALLALLIIFIVNFYWKISAHTTGMGGLLGAVMYINWVQYENLLWLYVA
                     IIICGALVSSARLQLKAHTSMQLLAGYSVGFACVGVLPWCVTMLFNVL"
     gene            7691..7762
                     /locus_tag="PK641_01270"
     tRNA            7691..7762
                     /locus_tag="PK641_01270"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7724..7726,aa:Glu,seq:ttc)
     gene            7793..8047
                     /gene="rpsT"
                     /locus_tag="PK641_01275"
     CDS             7793..8047
                     /gene="rpsT"
                     /locus_tag="PK641_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HPG55020.1"
                     /translation="MANHKSSVKRIRQTETKRLHNRYYAKTVRNAVRDIRAMNKKEEA
                     VASLSKVVSMLDKLARMNVIHKNKADNLKAKLALHINKLA"
     gene            8143..8841
                     /gene="radC"
                     /locus_tag="PK641_01280"
     CDS             8143..8841
                     /gene="radC"
                     /locus_tag="PK641_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HPG55021.1"
                     /translation="MEENCAVGIKAWSEDDRPREKLLQKGASVLSDAELISILIGSGT
                     RNESAVGLARRVMQKADNDLNILARLTPTELSNSIKGIGYAKAVTIIAAMELSRRRRK
                     VTQSEQDVIKESSDVYNIFLPDLGDLSYEEFWILLLNHANKVVAKVKIASGGVHQTIV
                     DVKLLFKYALDRLATGIILCHNHPSGNLFPSAADKLLTNKIVDGAKFLDIRILDHVII
                     GRDEYFSFADEHLL"
     gene            8884..10194
                     /locus_tag="PK641_01285"
     CDS             8884..10194
                     /locus_tag="PK641_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55022.1"
                     /translation="MKYIFEQIFEKILRQEIVIINDKLALSSYSCPILCYSRTETIPN
                     SFVVRPHGLLFGSGIKQYNPEVEDWNNTKIFFKTGNGDTDLPFDMFSAAFYLISRYEE
                     YGSTELNEFGCYKAESSLAYKNGFLEEPIVDQWAYLLENLLQRKNIGYEPAVKNRFVY
                     RPLLNLDRLYKYRKRSLSVTIYELVRKMINSKWVSLKHQLKVLSLQADDPYFNIDEVI
                     ELHNRNNLVPTFFLLVKKGIKYDNQIFFTFYYGIRKLLRRNFLVEFHPSYSSFGSYNR
                     MKIEKNFLEAKVIRARATMSLFHFRMVLPESYHNLLKLAIKEDFSMSYLNHIGFRAST
                     CTPFNFYDINHEYTTKLVVHPVEISDRTLKSMGIHHEMLEKLMKDYANKIKLVNGEMA
                     TSFQNVSLSNDGRWFGWRGEYESAIRYISLLENHSIEESIDLSK"
     gene            10201..10272
                     /locus_tag="PK641_01290"
     tRNA            10201..10272
                     /locus_tag="PK641_01290"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10234..10236,aa:Arg,seq:cct)
     gene            complement(10377..11849)
                     /gene="crtI"
                     /locus_tag="PK641_01295"
     CDS             complement(10377..11849)
                     /gene="crtI"
                     /locus_tag="PK641_01295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HPG55023.1"
                     /translation="MNKSVIIVGTGLGGLSTGLRLASRGYKVTFVEKAQQPGGRLNQL
                     KKDGFTFDVGPSFFSMPYEFEELVKDCGIVMPFEFVELDPLYTVNFRGSDKKYSLYKD
                     IDKLSKQFENVEPDFKTKFTAYLKKCESIYNDTVQVAIKQNFDSLTDYVIALSNVNPG
                     HLPVLLKSFWSQIGNYFKSKEARQIVSLVAFFLGRTPFDTNAVYTLLSHIEFKYTGYY
                     NVKGGMYTIVESLVEELKKFDVAFHYNTEIVDYVSDNDKLSSLIDQNKKSWQADIFVI
                     NSDAAYFRGTVFNRKKFAEKRLKRMSWTMGYLTFYVGLKTKLPQVEHHNYFLGNNYEE
                     YAKDVMKNPGTLEKPYYYVNVVSKHNPGCAPEGCEALFFVCPVPNLLHKTDWFDKQDM
                     VNSIIDDFSQRIKQDISKDIVFQMAYTPEDWQNQFNLYKGACLGLSHSMLQIGAFRPK
                     NWDEQFKNTFYVGASTVPGAGLPMAVIGSKLTTERILNFK"
     gene            complement(11842..>12376)
                     /locus_tag="PK641_01300"
     CDS             complement(11842..>12376)
                     /locus_tag="PK641_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55024.1"
                     /translation="GWSIGSNKNEEAEKYLKKATDNLNKLEEKKYKESMLFSYKAALI
                     GFEIGLAKYKAAFIGLNSLKYAKKSVSIDASNPLGYMQLGNVYYYMPKVFGGSKSLAL
                     EHYLKALKLMESSNDYKINNWNYLGLLVTIINAYTDLNQYEEAKKYCIKTLTIEPDFD
                     WVKDNLYPEILKKTKNE"
BASE COUNT         3959 a         1891 c         2388 g         4138 t
ORIGIN      
        1 tgaaggtagt aaccatggtt tatggtggtt tggtgaataa gaacatagta gcaggtttgc
       61 aaaaactagg tattaatgct ttgggtttga caggcgcaga tatggctgtg attaagagtg
      121 ataaacgacc tgttaaggat attgattatg gttatgttgg tgatgtaaaa tcagttgatg
      181 gcaaattatt ggcaaaccta attgaacagg gtgttgtgcc gattgtggct cctctaagtt
      241 tttcttctga atatggtttg cttaatacaa atgcagatac aatagctgca gaaacagcaa
      301 aagcattatc acaatttttt gacgtaaccc ttacatattg ctttgaaaag aaaggagtat
      361 taatggatga aaatgatgac gaaagcgtca ttcggacaat cactcctgat ttatttttag
      421 aatataagaa tgataatata atacagggtg gtatgatccc aaaacttgag aatgcttttg
      481 atgctttgaa agccggtgtt tctaaagttg tgataacaaa agcatcagag ttgggtaaag
      541 aatccggaac ctccgtactt gcataacaat aaagaaagta caattatgat agatattgca
      601 gcattactaa aacgtttgat agaaatacct tctactagta tgaatgaatc agccaaggcc
      661 gattttttat ataacgaatt aaaaaacaag gattgcgagc cgaatcgttt caaggataat
      721 ctatggtgta tggcatatcc ttatgatgct gcaaaacata ctatcatgct taattctcat
      781 attgacactg tgaagcctaa tgcctcatgg acttatgagc ctaacaaagc tacagaagtt
      841 gatgggcgta tatatggact aggttcaaat gatgcaaatg catcagtcgt agcattaatt
      901 gaaacctttt tgagattaaa gcaaacaaag cagtcttata atctaatttt ggctttatct
      961 gcgcaagaag aagtttccgg aaaagacggc attgagtgtt tgctgaatga attgcctaaa
     1021 attgattttg caattgttgg tgaacctacc gggatgcgtt tggctattgc agaaaaaggt
     1081 ttgatggttc ttgattgtgt tgcgcatggt atatcggggc atgctgctcg tgatgagggc
     1141 gataatgcta tttacaaggc aatgaaggat gtagaatggt tccgcacatt cgagtttcct
     1201 aaaatatcag atacactagg taaagttaaa atgtcagtta ccatagttaa tgcagggact
     1261 caacataatg tggttcctga tacttgcagt tttacggttg atgtgcgtct tactgattgc
     1321 tattcgcacg aagatgtatt atctattgtc agaaaaagtg tttcttgtga tgttacccca
     1381 cgttcaatgc gtttacgtcc ttcgggagtt cctctttcaa atttttttgt taagcgatat
     1441 ttagctaccg gtggttctgt gtttgggtct tctacaatgt ctgaccaagc cttaatgcca
     1501 tttgcatcag ttaaaatagg accgggtgat tctgcgcgtt ctcatactgc agatgaatac
     1561 atagaattat cagaaatttt caaagcagtt gacatatact ataatttgtt ggatggttta
     1621 atcataatgt agatatgtga atattattaa tacattcttt gaaaatctta tcttttttag
     1681 tttaaatttt gttagtaata ttaatatata tatatctgca aatgttttca cactagaaaa
     1741 catcaacaaa attattcaat taatttatta atttaacaga tttattatta tgaaaaaaaa
     1801 ttattttacc aattgcagtt gttttgacga tgctattttc tgcatgcagt tctaacgaac
     1861 ctctaataga tgatgttaca gaaaaatgct gggagtataa ttattcagca tttggctatt
     1921 cagtaaaaga ttattattgg gcaacagcta aagagtttgg tgaatataaa accacgttgg
     1981 agtctatgta cgggacagtc acatcataca aagctgtaag taaatcagaa tcagagtgtg
     2041 agtcgtcaga atattaaatt gttatttttt taatcagccg tttgtcttta ttgacaagcg
     2101 gctgattttt taccatgctt tatatggatg tttagaaagt gatgcgtttg tgtagcgttt
     2161 atcacttgtt acttctttat caacccattc aggtacaata aaagattcct gttcactgtt
     2221 aagttcaatt tcggcgacta tcaatccttc gttgcaatct agaaattcgt caatctccca
     2281 tattttgccc ccaatttctt gcttatatct tatctttaca attggctgat acaaggctaa
     2341 tttcatcata tcttgagcat cagctatagg aatttcatat tcaaattctt tcctggaaca
     2401 tccaaccgtt ggacctttta ttgtaataaa ggccttagtt tcggcaatgc gaactcttaa
     2461 tgtgcatccg tcttttgcta gataagcttg ggtgtatagg catcctgtat tgggcttatt
     2521 ccattttttc ttgtttacca agaatttttt ttctatttct attcccatta tttctttttg
     2581 tgcaaatgta ctagttttaa ctaaaatgta ctacctttgg ggtaaacaaa aaatatttaa
     2641 taattatgag agtaccgtct caatatgatg atattcgtag atattacgat gaggaagtaa
     2701 atcctgcgct tgccgaaata ttagcccaaa cacagatatc taaaatttta gcagcgattc
     2761 taggtggaga taatgccgag tatttcttac gtaccttagc tccaattaat tctatagacg
     2821 attttcaaag agaagtgata gtcgttttat tgactgctat agagcacaag acatgtactg
     2881 aagtgaagtt gtatggaact gaatatatag ataggagcaa gggtcatttg tttatgtcga
     2941 atcatcgtga tatagtactt gattctgcat ttttaaatgt gcatttgttt tataaagggt
     3001 ttaaaaccac gcagatagca ataggtaata atcttttgat ttatccatgg atagaacacg
     3061 cagtccgcat caataaaagt tttgttgtcc gcagggatgg ttctataaaa gagcaattgc
     3121 tgatatcaag aaaattatct gaatatatgc gaattgtttt gtcagaaaaa aaagaatcca
     3181 tatggttggc tcaacgtgag ggcagagcaa aagattcaaa tgatataact caatcatctg
     3241 taataaagat gcttaatatg agtggtcgca aatcgttttc agaaaatatg cgcgaattaa
     3301 atataactcc tgtttctata aattatgaat atgatccttg tgatttcttg aaagcaaagg
     3361 aatttcaaca gaaacgagat gatgcaaatt tcaagaaatc tcccggagat gatataaaaa
     3421 gtatgcagac aggtattatg gggcgaaaag gtcgtgtttc acttgttatt agtgagccat
     3481 taaatattga tgaagcatgg aacaaattgc ccaaaaacga acaagccgaa gcattgtcta
     3541 aggatcttga tacttctatt catcgtaatt atcttttgtt tgaaaataat tatattgctg
     3601 cagacctact aagtggcggg acagattttt caggtcatta ctcagcttct caagttgcta
     3661 cattcgtgcg ttatatcgaa aatcagcttt taaagattga tttacctaac cgagatgaaa
     3721 tgttcttgcg cagaaaaatg tatgagatgt atgccaaccc ggctataaat cagtcaaagc
     3781 tattgtaaaa tgagtgccat atcggagcaa taccggcgat atctgttgtt agaacgagca
     3841 ttctctccaa atacgatgga tgcctatatg cacgatgtgg acaaactttt ggattatctg
     3901 gacgcaaaaa atcttactta tcttacagca tcaaccgatg acttaagccg ttttttaatg
     3961 accttaaccg atttgggtat tgcatatagg tcacaagctc gcattgtgtc aggtattaaa
     4021 tccttttata agttcttgat tatggaagat ctcataaaag aaaatcctgc agatttgttg
     4081 gaattaccca aattagctaa aaaattaccc atagtattgt ctatagaaga aatcgataga
     4141 atagaaagtg ttatagattt atctaaacag gaagggcaac gtaataaagc aataatagag
     4201 actctttata gttgtggctt gcgtgtatca gaattagtta acttaaaact ttcgtctatc
     4261 tattgggatg aggaatattt aaaagtagag ggtaagggaa gcaaacaacg tttagttccg
     4321 atatcaaaaa cagccttaaa agaaattcgt aattatttac ctgataggaa tgctttgacc
     4381 ataactaaag gaaatggcga ttatgtgttc ttaaatagac gaggggctaa acttactcgt
     4441 caaatggttt ttatgtggct taagagatat gccggtgaag caggaattaa aacagttatt
     4501 agcccacaca cattgcgaca ttcgttcgct acacaccttt tagaaggcgg tgcaaacttg
     4561 cgtgtaatac aggaaatgct tgggcatgaa agcattttaa caacagaaat ttacacgcat
     4621 attaccatgg attatttaag aaaaaccata atggattgcc atcctcgaag cagccatatt
     4681 tagaaattgt gattttagat tataaattgc tatgtggaga ttgcaattgt aaaataaatt
     4741 atctaatttt gtaggactta atatactact ttatgaatat tttcgataaa cttactgcaa
     4801 atgctaagtc agagcttaaa cgtatcgttt tacctgaatc tttggaagag cgtacaataa
     4861 aagctgctga tatcgcattg gcagagggtt tggcagatat tgttttgctg ggtgagaaaa
     4921 aaactatttt ggcaaaagct aatgagttag gactttctaa tatagaacgt gctacaattt
     4981 ttgatgtaaa taccaatcct aaagctgctg agtatgtagg tttattatac gaattacgta
     5041 aagaaaaagg gatgactgag gacgaagcca agaaattagt ttatggtaat cctttatatt
     5101 tagcatcatt gatgataaaa aatggtgatg ctgatggtga agttgcagga gctatgaacg
     5161 ctacaagtaa tgtattgcgt ccggcattgc aaattattaa aatgaaaaaa ggtgttaatg
     5221 ttgtttcagg tgcattttta atgatagtac caaacgagag ttatggcgaa aaaggaatgt
     5281 ttttatttgc tgattgtgca gtaacccctg atcccacagc tcaacaatta gctgaaatag
     5341 cagttgtaag tgcatcaaca gctaaagttt ttggcgagtt tgatcctcgt attgcaatgt
     5401 tgagtttttc taccaaagga agcgcaaaac acgaaagcgt agaaaaagta caagaggcaa
     5461 cgcgtttagc aaaagaaatg tctcctaatt tgttgttaga tggagaattt caagcagatg
     5521 cagctatagt cccatctata ggaaaaagca aggctccaaa cagccctatt caaggaaatg
     5581 ctaatgtgtt gatattccct aatttggatg caggtaatat aggatataaa ttagtacaac
     5641 gtttatcagg aggtattgct ataggtccaa ttctacaagg tatggcatcg cctgtaaacg
     5701 acttatctcg tggatgtagc gttgatgata tagtcaagat gatagtaatc acatcaaacc
     5761 aaactcgttt ataaaattat catgaaaata ttggtattga attgcggtag ctcttctgtc
     5821 aaatttaaat tatttgacat ggataagaaa atcgttttga cacaaggtgg aattgaaaaa
     5881 ttaggaatga aagattcatt tttgaaaatg acattaccta atggagaaaa gaaggtattc
     5941 aatgatttta tgttagatca tcacgtagca attgctttaa ttctaaaaat attagtaagt
     6001 aaagaatatg gtgcgataaa gtctttagat gaaattgatg ctgtcggtca tcgtgttgta
     6061 cacggaggag agaattttaa taaaagtgtt attattacca aagaggtaat agacggagta
     6121 aaagcgtgta ttgacatggc tcctttacac aatccggcta atttagaggg aatatatact
     6181 atttcaaaaa tattaccgaa agttccgcaa gttgcagttt ttgatactgc ttttcatcaa
     6241 actatgcctg aatatgcgta tatgtacggt attccttatg aattatatca aaaatacaaa
     6301 gtacgtaggt acggatttca tggaactagt catagatatg tatccaagca tgtatgcgaa
     6361 tttttaggtg ttgaacagga agggaaacgc ataattactt gccatatagg taacggaggt
     6421 tcaattacgg ctattaaaga tggaaaatct attgatacgt caatgggatt tacgccactt
     6481 gaaggtttgt taatgggaac tcgttcggga aatgttgatc ctggagcctt agattttttg
     6541 gttgagaaag agcaattgac ttcggtttct ttatctaatt tgcttaacaa gaagagtggt
     6601 gtgttaggag tttctggagt ttcttcagac atgcgcgatt tggatgcagc agccaaagat
     6661 gggaatcata gagcagagct ggcacttaag atgtatgcat atcgtatttt gaaatacatt
     6721 ggagcatata cagcagtttt aggcgggttg gatatactgg tatttaccgg tggagtaggc
     6781 gaaaatcaaa tcgaaattcg tgctaaaata tgtagcggac taaaatatct tggaattgag
     6841 atagatgaca atagaaatca attacgtggt caagatgcta ttattagttc ggattcatca
     6901 agggttaaag tagcagtaat accaacagac gaagaattta tgatcgctat tgatacaatg
     6961 gaattagttc actgatgaga gaaaaaattg caaaaattct gacgctgctt tttcaaccaa
     7021 tatttgtgcc tctttatggc ttcatattgt tgtggaattc ttcttatttc tatccatatt
     7081 cgatttttat taaggtgatg gtattaggtt tatcatctct ctttatggta atattacctg
     7141 caatatttta tgcaatattg tataagaggg gaaaaattac cacacctcag gcatcaaata
     7201 gtaaagagcg tattccggta tacatgttca caatgtgttc ttattcaatg ctcatatact
     7261 tattgttata tatcggtgtt gtttattatt ttacttatat tgcgttaggt gcattgttgg
     7321 cattgcttat aatctttatt gtcaattttt attggaaaat aagtgctcat acaacgggta
     7381 tgggcggatt attaggagca gttatgtaca ttaattgggt gcagtatgaa aatttgttgt
     7441 ggctgtatgt ggcaattatt atatgtggtg ctttggtttc ttctgccaga ttgcaattaa
     7501 aagcgcacac gtcaatgcaa ttgttggcag gatattctgt cggatttgcg tgtgtggggg
     7561 ttttaccatg gtgtgtaact atgcttttta atgtcttgta gggattgttt tgtatattga
     7621 taaaaaaaca gaataaatat ttgcagataa tataaagaag tcgtatattt gcaccctcaa
     7681 aatcaaccat ggcccattcg tctatcggtt aggacgcaag attttcattc ttgaaagagc
     7741 agttcgattc tgctatgggc tacaaaataa aaacaattat tctttaaaag aaatggcaaa
     7801 tcataaatcg tctgtaaaaa gaatcagaca aactgaaaca aagagattac acaatcgtta
     7861 ttatgcaaaa acagttcgta atgctgttcg cgatattcgc gcaatgaata aaaaagagga
     7921 agctgttgca tctctttcaa aagttgtatc tatgttggat aagttggctc gtatgaatgt
     7981 tattcataag aataaagctg acaatttgaa agcaaagtta gctttgcata taaataaatt
     8041 agcgtagtag attattttta tcatttatag gctcttcttt aaggtaaaga agagcttttt
     8101 tttcgttatt ccattttaat ttttaatttt gaacttcttt ttatggaaga aaattgtgct
     8161 gtgggcatca aagcatggtc tgaagatgat cgcccaagag aaaaactatt acaaaaagga
     8221 gcatcagtat tgtctgatgc agagttaata tcaatcctga taggttctgg gactagaaac
     8281 gaaagtgcag taggtttagc aagacgagtt atgcaaaagg cagataatga tttgaatatt
     8341 cttgctcgtt taactccaac tgaattgtcg aattctataa aaggtatagg ttatgcaaaa
     8401 gctgttacta ttatcgctgc aatggaatta tctagaagac gtagaaaggt aactcaatcc
     8461 gaacaagatg ttataaagga gagttctgat gtttataata tttttttacc tgatttaggt
     8521 gacctttctt atgaggagtt ttggattcta ttattgaatc acgcaaacaa agttgttgcg
     8581 aaagtaaaaa tcgcaagtgg gggcgttcat caaactatag tggatgtgaa attgcttttt
     8641 aagtatgcat tagatcgttt ggcaaccggt ataatattat gtcacaatca tccatcgggg
     8701 aatttgtttc ccagcgcggc tgataagttg ctgacgaaca agattgtaga cggtgcaaag
     8761 tttttggata tacggatatt agaccatgtt attataggaa gagatgagta ttttagtttt
     8821 gctgatgaac atttattata aatcaatatg ctagctgttt tcacggaatt atacacccct
     8881 cgtgtgaaat atattttcga acagatattt gagaaaatac tgcgtcagga aatcgttatt
     8941 atcaatgata agctcgcatt gtcttcatat tcatgtccga tattgtgtta tagcagaaca
     9001 gaaacaatcc caaactcttt cgtagtacgt ccacatggat tgttattcgg ctcaggtatc
     9061 aaacagtata accccgaggt agaagattgg aataatacaa agattttttt taaaacagga
     9121 aacggtgata cagatttgcc atttgatatg ttttctgcag cgttttactt gatatcgaga
     9181 tacgaagaat atggttcgac tgaactaaat gaatttggtt gttacaaagc agagtcaagt
     9241 cttgcatata agaatggctt tctagaggaa cctatagttg atcagtgggc atatttatta
     9301 gaaaacttat tacagcgaaa gaatattggt tatgaacctg ctgttaaaaa taggtttgta
     9361 tataggccgt tattgaattt ggatagattg tataaatata gaaaacgatc attgtcagtt
     9421 accatttatg agcttgtaag aaagatgatt aattcaaaat gggtaagttt gaagcatcaa
     9481 ctaaaggttt tgtctcttca agccgatgat ccttatttta atatcgatga ggtaattgaa
     9541 ttacataata gaaataattt agttccaaca tttttcttgt tggttaaaaa aggaataaaa
     9601 tacgataacc agattttttt cacattttat tatggcattc gtaaattgtt acgtcgtaat
     9661 ttcttagtcg aattccatcc atcatattca tctttcggtt catataatag gatgaaaatc
     9721 gagaaaaatt ttttggaagc aaaagtaatt cgagctaggg ccacgatgag tctgttccat
     9781 ttcagaatgg ttctaccgga gagttatcat aacttactaa aattggccat caaggaagat
     9841 tttagtatgt cttacctaaa tcatataggt tttagagcga gtacatgtac tccctttaat
     9901 ttttatgata ttaatcacga atatacaaca aagctagtcg tacatcctgt ggaaatatct
     9961 gataggactt taaaatcaat gggaattcat catgaaatgc ttgaaaaatt aatgaaagat
    10021 tatgctaata aaatcaagct tgtcaatggc gaaatggcaa cgtcttttca aaatgtttct
    10081 ttatcgaatg acggccgttg gtttggatgg cgtggtgagt atgaatcggc gattagatac
    10141 atttctttac tagaaaatca tagcattgag gaatcaattg atttgtcaaa gtaaaatgtc
    10201 gctcctgtag ttcaacggat agaatagaag tttcctaaac tttagatagg ggttcgattc
    10261 ccctcgggag tacaaagtaa gcggctgtat atcaacgata tacagccgct tactatttta
    10321 acaaggtgca aattagttgg cttgcaaatg tgcttacaac tctgccttgg caaatgtcac
    10381 ttaaaattca gaattctttc tgttgtcaat tttgaaccaa taactgccat tggtaaacct
    10441 gctccaggca ctgttgatgc acctacatag aaagtgtttt taaactgttc gtcccagttt
    10501 ttagggcgaa atgcacctat ttgcaacata ctatgcgata aacctaagca tgctccttta
    10561 tatagattaa attggttttg ccaatcctca ggggtatatg ccatttgaaa aacaatgtct
    10621 ttggaaatgt cttgtttaat tcgttgcgaa aaatcgtcta ttatgctatt caccatatct
    10681 tgcttatcaa accaatctgt tttatggagc aaatttggaa cagggcaaac aaaaaataat
    10741 gcttcacaac cctctggggc gcaaccagga ttatgttttg atactacatt tacgtaatag
    10801 tatggttttt caagtgtacc cgggtttttc atcacatctt tggcatattc ttcgtaattg
    10861 ttacccaaga aatagttatg atgctctact tgcggtaatt tggtttttag tcctacatag
    10921 aacgttaaat agcccattgt ccaactcatt ctttttaaac gtttttccgc aaactttttg
    10981 cgattaaaaa cagttcctct aaaatatgca gcatccgaat ttataacgaa aatatcggct
    11041 tgccagcttt ttttattttg atcgattaat gaacttaatt tatcgttgtc gctcacgtaa
    11101 tcaacaattt cggtattgta atgaaatgct acgtcgaatt ttttaagttc ttctactaag
    11161 ctttccacaa tagtgtacat tccgccttta acattgtaat atccggtata tttaaattca
    11221 atatgcgaca gaagggtgta tacggcattc gtgtcaaatg gtgtgcggcc aagaaaaaaa
    11281 gcaacgagtg aaactatttg cctggcttcc ttagatttga aataattgcc tatttgagac
    11341 cagaagcttt taagtaaaac aggcaaatgg cctgggttta cattggataa ggctataacg
    11401 taatcggtta aactgtcgaa attttgtttg atagctactt gaacagtatc gttgtaaatc
    11461 gattcgcact tttttaaata tgcggtgaat ttggtcttaa aatctggttc aacattttca
    11521 aactgtttgc ttaacttatc aatatcttta tatagcgaat acttcttgtc gcttccccta
    11581 aaattgaccg tataaagtgg gtctaattca acaaattcaa acggcataac aatgccacaa
    11641 tctttaacaa gttcttcgaa ctcgtatggc atactaaaaa aacttggacc tacatcaaaa
    11701 gtaaatccgt ctttttttaa ctggtttaaa cgaccgccgg gttgttgtgc tttttcaacg
    11761 aaagttactt tatatcccct gcttgctaat cgcaatcctg tagaaagacc tcccaagcct
    11821 gttcctacaa ttattacact cttattcatt ttttgtcttt tttaaaattt ccggataaag
    11881 attgtcttta acccagtcga aatcgggttc aatggtcaat gtttttatac aatatttttt
    11941 tgcttcttca tattgattca aatctgtata agcatttatt atagttacta gtaaacctaa
    12001 atagttccaa ttgtttattt tgtaatcatt gcttgattcc attaatttta aggcctttaa
    12061 gtaatgttct aaagctaaac tttttgaacc gccaaaaact tttggcatgt aataatatac
    12121 atttccaagc tgcatatatc ctaatgggtt tgaggcgtcg atgcttactg attttttggc
    12181 atatttcaga ctattcaatc ctataaaagc agctttatat tttgccaatc caatctcgaa
    12241 tccaattaaa gctgctttat atgaaaatag cattgattct ttgtactttt tttcttccaa
    12301 tttattcaaa ttgtcggttg ctttttttag gtatttttct gcttcttcat ttttattact
    12361 gccgatgctc catccg
//
LOCUS       DAOPID010000016        12241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_6164_length_12241_cov_4.434761, whole genome shotgun sequence.
ACCESSION   DAOPID010000016 DAOPID010000000
VERSION     DAOPID010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 12241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12241
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6164_length_12241_cov_4.434761"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1678
                     /locus_tag="PK641_01305"
     CDS             <1..1678
                     /locus_tag="PK641_01305"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HPG55025.1"
                     /translation="LGLPGQSAIFSQRHETSLVGDYIFRNPYLPYFKATENVTFYNTR
                     VPYSNLSYYTGGYTNHGEDHLTGILSMNVNKRLNFGLDVNYIYGRGSFYNQSSDGLTG
                     ALNGSYRGEKYSIYFIAGLNNFRNFENGGITNDSDLKSMTDSYNVPVRINNAWSVYNS
                     FYIWVNQQYSIGFDKLDPDDSEKKTFVPVTTFGYTVKFEGSRKKYYERSIPANFYANN
                     YYSDKNTRDTTSNNILRNILSVTLNEGFQKWAVFGVRAFAEADVEENMCLIADSIYGY
                     QTQCLVSVGGELFKRKGKTTYGVLGEVLLLGSKNRLAFNVSGDFNTEIPIKNDKLLIS
                     AVGHVKSTNPSHFTETYYSNHFIWENKFDNTWSARGYGNIGIPNKYCNFSIGAGWQGL
                     KNYIYFGNDGTPKQSTDFIQIISGNVRCDVHVKWFHFENQATIQYSTNKDILPLPLVS
                     IYSNLYFKWLVFKKILTLQVGVDCRYNTAYYANAYMPATGQFYLQNETLVGNYPLMNV
                     YLNMHLKQFRVFLMYYNFSQIFMEPTYFAVPHYPLNPGMFKVGISWNFYN"
     gene            1684..2535
                     /locus_tag="PK641_01310"
     CDS             1684..2535
                     /locus_tag="PK641_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPG55026.1"
                     /translation="MDELLKKMAKRKHIIISICCLLLVFVIAIFMAFYKNNKAMFAHD
                     YPQIVKNGTLKVIVEPNKMSYRVIDMDSIVGLQVKMIERFAEDHNLKVEFVEENDLSK
                     AMEMLDANEIDVLVWHIPVYDQLKNKISYTIPVFTSRQMLIQRKKSKTDTTQFIRNQL
                     DLAGKTLYILPGTIYKQRIEHLSKEIGDTIHIKEIPNADPSDLFKMIVEKNIDYTVCD
                     EFVARVLLKDFPTIDMGTAVSFTQNYSWGINPASTILKDSLDSWLAIYLESKDYNRIY
                     RQYTGVP"
     gene            2613..3347
                     /locus_tag="PK641_01315"
     CDS             2613..3347
                     /locus_tag="PK641_01315"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPG55027.1"
                     /translation="MIQISTSYGNITVKLYDETPLHRDNFIKLVKQGFYNDLLFHRVI
                     KDFMIQGGDPQSKNAPAGKQLGAGDVGYTIPAEFAYPKYYHKKGALAAARQGDQVNPK
                     KASSGCQFYIVEGKTYNDKEIEQLENRMLNTAKQTRFYQLANEHSDEIKNLRIKKDSV
                     GIEKLQNQLISQLESEFANKSYKMPEQMKNDYKTIGGTPFLDNEYTVFGEIVEGMEVI
                     DKIAAVEKNASDRPKQDIKMTVKILE"
     gene            3369..4082
                     /locus_tag="PK641_01320"
     CDS             3369..4082
                     /locus_tag="PK641_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HPG55028.1"
                     /translation="MITISEIKEELLRNAIPEKKAILSSFFKTGKGQYGEGDKFIGVS
                     VETQRSLLKRVKSIELSEIEILIADEYHECRLTALLLLVDMYKKAKPDQKQAIFDLYC
                     KSTKYINSWDLVDLTAPNIVGTHLENQSRELLYKWANSDLLWEQRISIVSTIIYIRKN
                     DFKDALSIISILQNNKHDLIQKAAGWMLREIGKRNFEVEYKFLTENNRYKELPRTLLR
                     YAIEKFPEEMRQKFLHSEI"
     gene            4100..4996
                     /locus_tag="PK641_01325"
     CDS             4100..4996
                     /locus_tag="PK641_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPG55029.1"
                     /translation="MAHEHHHHDHGATIDSLNKAFIIGIAINALYVIIEAVAGFAYGS
                     IGLLSDAGHNLGDTASLILALIAFKLAHLKANNKYTYGYKKSTILVSLVNAVILLVAV
                     GIIIAESIEKFFNPQAIEGDVIAWVAGAGVIVNAFTAWLFMKDKEKDLNVKGAYLHMA
                     MDALVSVGVVISGIVINYTGWYVIDPIIGLIVACIITVSTWNLLHDSLRLSLDGVPSG
                     IDLKQIEKIICSDEGVKSCHHLHVWAISTTQNALTVHIVPSSIDKMETIKERIKKNLL
                     KANISHSTLEFEQSDSHCDEHC"
     gene            complement(4993..5280)
                     /locus_tag="PK641_01330"
     CDS             complement(4993..5280)
                     /locus_tag="PK641_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4491 family protein"
                     /protein_id="HPG55030.1"
                     /translation="MNFIGLAIGICTFLIIGLFHPIVIKFEYYFGVKIWWVFLLCGIL
                     FTVLSLFLSSTFWSVLAGVFAFSSFWSIKEIFEQRERVRKGWFPKNEKRKN"
     gene            5527..6108
                     /locus_tag="PK641_01335"
     CDS             5527..6108
                     /locus_tag="PK641_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007752210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HPG55031.1"
                     /translation="MKILLIDNNDSFTYNLLHLIKSVCNNNDSVEVCSSNGITEKKLA
                     DSTRIIISPGPGTPAEAKKFHKIIRSYEQQIPILGICLGHQIIAESYGAVTYNLTRPA
                     HGIQSEISITDHTDIFRGMQSNIMAGRYHSWCVSSENLPDCLKITAADNAGNIMAISH
                     CEYNIFGVQFHPESFMTDYGKIIMSNFLNLNQL"
     gene            6105..7073
                     /locus_tag="PK641_01340"
     CDS             6105..7073
                     /locus_tag="PK641_01340"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HPG55032.1"
                     /translation="MRAVEVKKRMNDCGRMKTPFLFAVDFDMKEGIFIENPLHQTEVL
                     FRTPTAKVSQQSKLLSTFIDLSAQPITLSEYKDRFDIIMQGLMRGDSYLTNLTVRTPV
                     QTNLSLEEIFLLSNSPYGLYIPGKFVCFSPERFIRIANDTISTNPMKGTISAEVSNAK
                     CVILGDPKETAEHNTIVDLMRNDIGMVAEDVQVERFRYIDRIKTHNGDILQVSSEITG
                     RLPKDNLSHLGDIVFRMLPAGSVSGAPKQSTVNLIHKAEPEARGYYTGVFGYFDGSEL
                     DTAVLIRFIVQDENKLFFHSGGGITINSDHKIEYDEVLKKIYLPII"
     gene            7070..7669
                     /locus_tag="PK641_01345"
     CDS             7070..7669
                     /locus_tag="PK641_01345"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HPG55033.1"
                     /translation="MKLQFSEAIKIKDGVIYNLPYHQARIDRTLTKFDGLKIDLVSTL
                     TSITDDVNKGLFKCRLIYGKAGVENIEFIPYSPRRITTVRPITADNIEYGYKYTDRAH
                     LNELLEQSGCDDIIIVKNGLVTDAFAANLIFESPDGLFTPDTYLLPGTKRQYLLDMGI
                     IRQKRITVADIKFYDRIRFISAMIDLEDNICINSFHVRI"
     gene            7857..9695
                     /gene="rpsA"
                     /locus_tag="PK641_01350"
     CDS             7857..9695
                     /gene="rpsA"
                     /locus_tag="PK641_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HPG55034.1"
                     /translation="MAKELKQNAQGVTDFDWNAYENGAVNTAMSKEDLQKAYDKTLNQ
                     INTNDVVMGTVISMNKREVVVNIGYKSDGIVSMNEFRYNPELKIGDKVEVYIESQEDK
                     KGQLILSHKKARSTRSWDRVNEALEKDEIIKGYVKCRTKGGMIVDVFGIEAFLPGSQI
                     DVKPIRDYDIFVNKTMEFKVVKINHEFKNVVVSHKALIEAELEQQKKEIISKLEKGQI
                     LEGTVKNITSYGVFIDLGGVDGLIHITDLSWGRISHPEEILQLDQKLNVVILDFDDEK
                     KRIALGLKQLTPHPWEALNTNLKIGDKVQGKVVVVADYGAFVEIAPGVEGLIHVSEMS
                     WSQHLRSAQDFVKVGDTVEAVILTLDRDERKMSLGIKQMKEDPWLNIEAKYTIGSKHT
                     AKVRNFTNFGVFVELEEGVDGLIHISDLSWTKKIKHPSEFTTLGAEIEIVVLEIDKEN
                     RRLSLGHKQLEENPWDTYETIFTVDSIHEGTIIEMMDKGAVVSLEYGIEGFATPKHLV
                     KEDNSQAKLDDKLQFKVIEFNKEAKRIILSHSRIFEDAQKSAANEQAPEKKAKKSTKK
                     EEMPTMEKTTLGDIEGLAALKDELTKSEAKTAEKKASKKAKSESAE"
     gene            complement(9764..>12241)
                     /gene="uvrA"
                     /locus_tag="PK641_01355"
     CDS             complement(9764..>12241)
                     /gene="uvrA"
                     /locus_tag="PK641_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015533032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPG55035.1"
                     /translation="VEQKVKTRNSRSTVGTSTEIYDYLRMLYARIGKTISPISGVEVK
                     KHQVKDVLDYIVSLPEGENTVLTAPVLDVNQIPIWKMQGFSRLYVNNEFIRIDEFSTY
                     DKSYERLFLVVERFSVQKDQSFINRMADSVETAFYEGHGICYVGDTEFSRNFEADGIS
                     FEEPTEQLFSFNSPIGACPSCQGFGKIIGIDEDLVVPDKSLSIYEDAIACWRGEKMGD
                     DKWDLIHNAEKFDFPVHKPYYELTDKQKQLIWTGNEYFKGLNKFFKYLESYQYKIQFR
                     VILSRYRGKTICPTCNGTKLKKEALYIKVCGKSISELVEMPIYALLDFFKNAEFSEQE
                     KQIAKRLLIEINNRLQYLIDVGLEYLTLNRLSNSLSGGESQRINLATSLGSSLVGSLY
                     ILDEPSIGLHAHNTELLIKVLRQLKDLGNTVVVVEHDETIMRAADWIIDIGPGAGRLG
                     GELVVAGNPSDLLSKYKKGSTSHTLRYLAGEDTMKVPLQRRKWSNYISIEGATQNNLK
                     NLTVCFPLNVMTVVTGVSGSGKSTLVRDLFYSGLKRYYGGAIEKVGSFASLKGSLSLI
                     NDVEFVDQNPIGKSSRSNPCTYIKAFDEIRKLYAEQALAKQMSFSAAYFSFNSQGGRC
                     EECQGEGTITIPMQFMADVKIECEACHGKRFKQEILDVKYRGTDIYQVLSMTVNQAID
                     FFGEDKAASARKIVSRLLPLQEVGLGYIKLGQSSSTLSGGESQRVKLAAFLSSNNGEK
                     RIFVFDEPTTGLHFFDIKTLIKAFDSLIEKGHTVIIIEHNLDVIKQADHIIDLGPEGG
                     ENGGEVIFEGTPEEIKKCSRSITGKYL"
BASE COUNT         4313 a         2184 c         2056 g         3688 t
ORIGIN      
        1 cttaggctta cccggacaat cggctatatt ctcacaacgt cacgaaacat cattagttgg
       61 cgattacata tttcgaaatc catatctgcc ctattttaaa gcaacagaga atgttacatt
      121 ttataataca cgcgttccat atagtaattt atcatattat accggtggat acactaacca
      181 tggagaagac catttaaccg gtatactctc aatgaatgta aacaaaagac tgaactttgg
      241 gcttgatgta aattacattt atggaagagg ttcattctac aatcaatctt cagatgggct
      301 cacaggagct ttaaatggta gctatcgcgg agagaaatat tcgatttact ttattgccgg
      361 acttaataat tttagaaatt ttgagaacgg cggtataaca aatgattcag acttaaaatc
      421 aatgacggat agttataatg ttcccgtcag aattaataat gcatggtctg tttataattc
      481 tttttatatt tgggtgaatc aacaatattc tataggtttt gacaaattag atcctgatga
      541 ttctgagaag aaaacatttg tgcctgtaac tactttcggc tatactgtca agtttgaagg
      601 aagcagaaaa aagtactatg agaggtctat ccctgcaaat ttttatgcaa ataattacta
      661 ttcagacaaa aacacgcgcg atacaacttc aaataatatt ctccgaaata ttttatcggt
      721 gactttaaat gaaggattcc agaaatgggc tgtatttgga gtacgcgcct ttgccgaagc
      781 cgatgtagaa gaaaacatgt gcttgatagc cgactctatt tatggatatc aaacacaatg
      841 tcttgtatct gttggtggcg aattatttaa acgaaaaggc aaaaccactt atggagtgct
      901 tggagaagtt ttacttcttg gtagcaaaaa ccgattggct ttcaatgtat caggagattt
      961 taacacagag atccctatta aaaatgacaa actgcttatt tcggcagtcg gacatgtaaa
     1021 aagtacaaat ccaagccatt ttacagaaac ttactattca aaccatttta tatgggaaaa
     1081 caaatttgac aatacatggt cggcacgcgg ttatggtaat ataggaatac ctaataaata
     1141 ttgtaatttt agcataggtg caggatggca gggtttgaaa aattacatat acttcggcaa
     1201 tgatggaaca cctaaacaat ctactgattt tatacaaatc ataagtggta atgtaagatg
     1261 cgatgtacac gttaaatggt ttcattttga gaatcaagca acaatacaat attcgaccaa
     1321 taaagacatc cttccattgc ctttggtatc tatatattca aacttgtatt ttaaatggtt
     1381 ggttttcaaa aaaatactta ctttacaagt tggcgttgat tgtagatata atacagcata
     1441 ctatgctaat gcatatatgc ctgctacagg ccaattctat ttacaaaacg aaactttagt
     1501 cggtaattat cctctgatga atgtatattt aaatatgcac cttaaacaat ttagagtatt
     1561 cttgatgtat tacaattttt ctcagatatt tatggaacct acatattttg cagttcccca
     1621 ctacccgctt aatccgggta tgtttaaggt tggcatctct tggaattttt ataactaatt
     1681 attatggacg aattattaaa aaagatggca aaacgcaaac atattattat atcaatatgt
     1741 tgccttttac ttgtatttgt aattgctatt tttatggcat tctacaagaa caacaaagct
     1801 atgtttgcac atgattatcc gcaaattgtc aaaaacggca cgctaaaagt aattgttgag
     1861 ccaaacaaaa tgagttaccg agtaattgat atggatagca ttgtcggttt acaagtaaaa
     1921 atgattgaac gatttgcaga agaccacaat ctaaaagttg aatttgtaga agaaaatgat
     1981 ttgtctaaag caatggaaat gcttgatgca aatgaaattg atgtattagt atggcatatt
     2041 cccgtatacg accaactaaa aaacaaaatt agttatacca tacctgtttt tacaagcaga
     2101 caaatgctta tacaacgtaa gaaaagtaaa acagatacta cccaatttat caggaatcaa
     2161 cttgatttag caggcaaaac actatatatt ttgcctggta ccatatacaa acaacgaatc
     2221 gagcatctat caaaagaaat tggagataca atacatatca aagaaatacc aaatgctgat
     2281 ccatcagatt tatttaaaat gattgttgaa aagaatatcg actacacggt atgtgatgaa
     2341 tttgtagctc gagttctact aaaagatttc cctacaatag atatgggaac ggctgtcagt
     2401 tttactcaaa actattcttg gggaataaat cctgcatcaa ctatcttgaa agatagttta
     2461 gatagttggc ttgcaattta tttggaaagc aaagattata accgcattta tagacaatac
     2521 accggtgttc catgaaaaaa agattaatat gcatagcatg ttttattata agcattctta
     2581 gttgctgtta ttcacaatta aaaacaaata caatgattca aatcagtaca tcttacggca
     2641 atattacagt taaactttat gatgaaaccc cattacatcg cgataatttt atcaaattag
     2701 taaaacaggg gttttataac gacttacttt tccatcgtgt aataaaagat tttatgatac
     2761 aaggaggcga ccctcaatca aagaatgcgc ctgccggtaa gcaattgggg gccggtgatg
     2821 tcggttacac tattcctgca gaatttgcat atcctaaata ttatcacaaa aaaggagcat
     2881 tagctgctgc cagacaaggc gatcaagtta atccaaagaa agcatcatca ggatgtcagt
     2941 tttatattgt tgagggtaaa acttataacg acaaagaaat agaacaactt gaaaacagaa
     3001 tgttgaatac ggcaaaacaa actcgtttct accaattagc aaacgaacat tctgacgaaa
     3061 ttaaaaatct tagaatcaag aaggattctg ttggtattga aaaacttcaa aatcaactaa
     3121 taagtcaatt agaatctgaa tttgcaaaca aatcttataa gatgcctgaa cagatgaaaa
     3181 atgattataa aacaataggt ggaactcctt ttcttgacaa tgaatatacc gttttcggcg
     3241 aaattgtaga aggtatggag gtgattgata agattgcagc cgtagaaaaa aatgcttcag
     3301 acaggcctaa gcaagatatt aaaatgactg taaaaatact tgaataaggt ctaattgttt
     3361 ttattaatat gataacaata tctgaaataa aagaagagct tctccgaaat gctattcccg
     3421 aaaagaaagc tattttatca tcttttttta aaaccggtaa aggacaatat ggcgaaggtg
     3481 ataaatttat aggtgtttcg gttgaaacac aacgaagctt gttaaagagg gtaaaaagca
     3541 tagaactatc tgaaatagaa attcttattg cagacgaata tcatgaatgt aggttgactg
     3601 cattgttgtt attagttgac atgtataaga aggcaaaacc agaccaaaaa caagcaatct
     3661 tcgacttata ttgtaaatca acaaaatata ttaatagttg ggatttggtt gatctaactg
     3721 ccccaaacat tgttggtact catttagaga accaatcaag agaacttcta tacaaatggg
     3781 caaacagcga tctactatgg gaacaaagaa tatcaattgt atctaccata atatatattc
     3841 gcaaaaatga ttttaaagat gcattatcaa taatctcaat tttacaaaac aacaaacatg
     3901 atttgataca aaaagctgca ggttggatgc ttcgcgagat cggcaaacgt aattttgagg
     3961 tcgaatacaa attccttaca gaaaataata gatacaaaga acttccacga actcttttgc
     4021 gttacgctat agaaaaattt ccggaagaaa tgcgtcaaaa atttttacat tcggaaatat
     4081 aatttataga cacaataaca tggcacacga acatcatcat cacgatcacg gagctacaat
     4141 tgactcttta aacaaagcgt ttatcatagg tattgcaatc aatgcgcttt atgttattat
     4201 agaagcagtt gccggatttg catacggatc tatcggtcta ctttcggatg ccggtcacaa
     4261 tcttggcgac acagcaagcc taattcttgc tttaatagct tttaaactag ctcatttaaa
     4321 agcaaataac aaatacacat atgggtacaa aaaaagcacc atattggttt cattggtaaa
     4381 cgctgttatt ttattagttg cagtaggaat aattattgca gaaagtattg aaaaattctt
     4441 caatcctcag gctattgaag gtgacgtaat tgcatgggtt gccggagccg gagtaatagt
     4501 aaacgcattt accgcttggc ttttcatgaa agacaaagaa aaagacctta atgtaaaggg
     4561 tgcatattta cacatggcga tggatgcctt agtatctgtt ggagttgtaa tttcgggtat
     4621 tgtcattaat tatacaggat ggtacgttat tgaccccata ataggtttaa ttgtagcatg
     4681 catcataact gtttcaacat ggaatctttt acacgacagc ctaaggcttt cgttagatgg
     4741 cgtaccttca ggaattgatt taaaacaaat tgaaaaaatc atttgctcag atgaaggagt
     4801 aaagagttgc catcacttac atgtttgggc aataagtaca acacaaaatg ccttaaccgt
     4861 acatatcgtg ccaagttcaa ttgacaaaat ggaaacaatc aaagaacgaa tcaaaaagaa
     4921 cttattaaaa gcaaacatca gccattcgac actcgaattt gaacagtcgg acagtcattg
     4981 tgatgagcat tgctaatttt ttcttttttc atttttagga aaccagccct tacgaacccg
     5041 ttctctttgt tcaaaaattt cttttatcga ccaaaagctc gaaaatgcaa atactcctgc
     5101 taataccgac caaaatgtgc tgctcaaaaa tagagataac actgtaaata atattccaca
     5161 caacaaaaat acccaccata ttttaactcc aaaatagtac tcaaacttaa taacaatggg
     5221 atgaaataag cctattataa ggaatgtaca tattcctata gcaagtccga taaagttcat
     5281 gttttttatt gtaaaggtaa taaaaacata tttctcatgt ttatttttgc tcaatatttt
     5341 atttgaatta atatattttc ataagaccac ttatgagaaa tttatattga cattaacaaa
     5401 tataaatgta aacggcttga ttttatccat ttttacgttc gggtatggtt ggcaaaagat
     5461 aaccgaaagg atttattgct gcagtgtgaa tgaaaggact tatctttgca aagtaattgc
     5521 ttatatatga agatactttt aatagacaat aatgattcat tcacatataa tctgctacat
     5581 ctaattaagt cggtttgtaa taataatgat tcagtagaag tatgctctag taatgggatt
     5641 actgaaaaaa aattagccga ttccacccga ataattatat ctcccggccc cggcactcct
     5701 gccgaagcaa agaaatttca taagattatt cgcagttatg aacaacaaat accaatatta
     5761 ggtatctgcc ttgggcatca aattattgca gaaagctacg gagctgttac ctataatctg
     5821 acacgaccag ctcatggtat tcaatcggaa atctcgataa ccgatcatac cgatatattc
     5881 cgaggaatgc agagtaatat aatggcaggc agatatcatt catggtgcgt ttcgagtgag
     5941 aacctacctg attgtttaaa aataactgct gctgataacg ccggaaatat tatggctata
     6001 tcgcattgtg aatacaatat attcggagtg cagtttcacc ccgaatcgtt catgactgac
     6061 tatggaaaaa tcattatgtc gaacttctta aaccttaatc aactatgaga gcagtagaag
     6121 taaaaaaacg catgaacgat tgtggacgga tgaaaactcc gtttttgttc gctgtggact
     6181 tcgatatgaa agagggaatt tttattgaaa acccattgca tcaaacagaa gtattgtttc
     6241 gcacaccgac agctaaagtc tcgcaacaat caaagctatt atcaacattc atagacttat
     6301 ctgcacagcc gataacatta tcagaataca aagatcgctt tgatattatt atgcagggat
     6361 taatgcgcgg ggattcatat ttgactaatc ttacagtcag aacaccagta caaacaaatt
     6421 tgagtttgga agaaatattt ctgttgagta attcgccata cggactttat ataccgggaa
     6481 aatttgtctg cttttcgccc gaacgattta taaggatagc aaatgacaca atatctacca
     6541 atcccatgaa aggaacaata agtgccgagg tttctaatgc taaatgtgtt atacttggcg
     6601 acccaaaaga aacagcagag cataatacga ttgtagattt gatgagaaac gatatcggta
     6661 tggtggccga ggatgtgcaa gttgaacgat tccgttatat agaccgaata aaaacccata
     6721 acggagacat tttacaggtc agttccgaaa tcacgggtcg attacctaaa gataatttgt
     6781 cacacttggg cgatatcgta tttcgaatgc ttccggcagg ttcggtttcg ggtgctccaa
     6841 aacaatcaac ggtcaatctg attcataaag ccgaaccgga agctaggggt tattataccg
     6901 gagtattcgg ttattttgat gggagcgagt tagatacagc cgtgcttata cgttttattg
     6961 ttcaagacga aaacaaacta tttttccata gtggcggcgg tattactata aatagtgatc
     7021 acaaaatcga atacgacgaa gtattaaaga aaatttatct gccaattata tgaaactgca
     7081 atttagtgaa gcgataaaaa tcaaagacgg cgttatttac aacttgccat atcatcaggc
     7141 tcgtatagat aggactttaa cgaagttcga cggcttaaaa attgacctcg tttcgacttt
     7201 aaccagcata accgatgatg ttaataaagg gctgtttaaa tgcaggttga tttacgggaa
     7261 ggccggcgta gaaaatatag agtttattcc atactccccg cgccgtataa ctactgtcag
     7321 accaattact gcagacaata tcgaatatgg ttataaatac acagatcgcg cccacttaaa
     7381 cgaacttttg gagcaatcag gctgtgacga tataatcatc gtcaaaaatg gcttagttac
     7441 cgatgctttt gccgctaact taatttttga atcgcctgac ggtttgttta cgcctgatac
     7501 ttacttatta cctggcacaa aacgccaata tttgcttgat atgggaataa tcaggcaaaa
     7561 acgtattaca gttgcagata taaaattcta tgatcgtatc agattcatta gcgctatgat
     7621 cgatttggaa gataacattt gtattaattc ctttcatgta aggatataaa caatacccgt
     7681 attggcacaa ataggcttat gtagtagtgt tatactgcta tctgcaatta attctatttt
     7741 gtaatataac ttttttataa atagttgatt atcccaattt ttatttctac ctttgccgct
     7801 cgttttgaga attttataca taaagtcaaa caactaatta tttttttaac aaacaaatgg
     7861 caaaagaact aaaacaaaac gcacaaggag ttacagactt cgattggaat gcgtatgaaa
     7921 atggtgcagt taacactgca atgagcaaag aagatttgca aaaagcttac gacaaaacct
     7981 taaaccaaat caacacaaac gatgttgtta tgggtacagt tatttcaatg aacaaacgtg
     8041 aggttgtcgt aaatataggt tacaaatctg acggtatcgt ttctatgaac gaattccgtt
     8101 acaatcctga attaaaaata ggcgataaag ttgaagtata catcgaatcg caagaagata
     8161 aaaaaggaca attaatcctt tctcacaaaa aagcacgttc aacacgttca tgggaccgtg
     8221 ttaacgaagc tcttgaaaaa gacgaaataa taaaaggtta tgtaaaatgt cgtacaaagg
     8281 gtggtatgat tgttgatgta tttggaatcg aagcattctt gccgggttct caaatcgacg
     8341 ttaaacctat ccgcgactac gatatctttg ttaacaaaac aatggaattc aaagttgtta
     8401 aaatcaacca cgaatttaaa aatgttgttg tatctcacaa ggctcttatc gaagccgaat
     8461 tggaacaaca gaaaaaagaa atcatctcta aacttgaaaa aggacaaatc cttgaaggta
     8521 cagttaaaaa catcacatca tacggtgtat ttattgactt gggtggcgta gatggtttga
     8581 tacatattac agacctatca tggggacgta tctcacatcc tgaagaaata cttcaattag
     8641 atcaaaaact aaacgttgtt attcttgatt ttgatgatga gaaaaaacgt atcgcacttg
     8701 gtcttaaaca attaactcct catccatggg aagcacttaa cacaaactta aagatcggtg
     8761 acaaagtaca aggaaaagtt gttgttgttg ctgattatgg tgcatttgtt gaaatagctc
     8821 ctggtgttga aggtttaatc cacgtttcag aaatgagctg gtcacaacac ttacgttctg
     8881 cacaagattt cgtaaaagtt ggagatacag tagaagctgt tatattaact cttgaccgcg
     8941 acgaacgcaa aatgtctttg ggtatcaaac aaatgaaaga agacccatgg ttaaatatcg
     9001 aagctaaata tactatcggt agcaaacata cagcaaaagt tcgtaacttt acaaactttg
     9061 gtgtattcgt tgaattggaa gaaggcgtag atggcttaat tcatatatct gacttatctt
     9121 ggacaaagaa aattaaacat ccatcagaat tcacaacact tggtgctgaa attgaaatcg
     9181 ttgtattgga aatcgacaaa gaaaatcgtc gtttaagcct tggacacaaa caattagaag
     9241 aaaatccatg ggatacatac gaaacaatct ttacagttga ttctatacac gaaggtacaa
     9301 ttattgaaat gatggataaa ggtgctgttg tttcattaga atacggtatc gaaggctttg
     9361 caactcctaa acatcttgta aaagaagata attcacaggc aaaattggat gataaattac
     9421 aattcaaagt aattgaattt aacaaagaag caaaacgcat tatcttatca cacagccgta
     9481 tatttgagga tgctcaaaaa tcagctgcca atgaacaagc accggagaaa aaagctaaaa
     9541 agtctacaaa gaaagaagaa atgccgacaa tggaaaaaac cacattaggt gacatcgaag
     9601 gcttagctgc tttgaaagat gaattaacaa aaagtgaagc taaaactgca gagaaaaaag
     9661 cttcaaaaaa agcaaaatca gaaagtgctg aataagacta actgacatac aaaaaaaata
     9721 ggatgctaat aattagcatc ctattttttt tgttttagct ttcttataaa tactttcctg
     9781 ttatagaccg actacatttt tttatttctt ccggtgtccc ttcaaaaata acttctccac
     9841 cattttcacc tccttctggt cccaagtcaa ttatgtgatc tgcctgtttt attacatcca
     9901 aattatgttc aataataatt acagtatgac ctttctctat taaactatca aaagccttta
     9961 taagagtctt aatatcaaaa aaatgcaaac cggtagttgg ctcatcaaaa acaaaaattc
    10021 ttttttctcc gttatttgaa gataaaaatg ctgccaattt aactctttgg ctttcaccac
    10081 cggacaaagt actagaagac tgcccaagtt taatgtaacc taggccaacc tcttgcagtg
    10141 gtaacaaacg gctaacaatt tttcgagcac tcgcagcttt atcttcacca aaaaaatcta
    10201 tagcctgatt aactgtcatt gaaagaactt gatatatatc agttccccta tattttacat
    10261 ctaaaatttc ttgcttaaat cttttaccat ggcaagcttc acactcaatt tttacgtcag
    10321 ccataaactg cattggtatg gttattgtac cctcaccttg acattcttca catctgccgc
    10381 cctgagaatt aaatgaaaaa tatgcagcag aaaagctcat ttgctttgcc aaggcctgtt
    10441 ccgcatacaa ttttcttatt tcgtcaaatg ccttaatata agtacatgga ttagatcttg
    10501 aacttttgcc aataggattc tgatctacaa attcaacatc attaatcaaa gacaaactac
    10561 cttttaaaga ggcaaaacta cctacttttt caatagctcc accataataa cgtttcagac
    10621 ctgaataaaa taaatcacgc actaaggtag attttccgga accgctcacg ccggtaacaa
    10681 cagtcataac gttcaacgga aaacaaactg tcaagttttt taaattattt tgagtagccc
    10741 cttctatcga tatatagttt gaccatttac gtctttgtaa tggcactttc atcgtatcct
    10801 ctcctgctaa atatcgcaaa gtatgcgatg tcgatccttt tttatattta gatagtaaat
    10861 cacttggatt acctgctaca acaagttctc cacccaatct ccctgcaccc ggacctatat
    10921 caataatcca atctgcagcc ctcattattg tttcatcgtg ttcaacaaca acaacagtat
    10981 ttccaagatc ctttaattga cgcagaacct ttataagtaa ttcggtatta tgcgcgtgta
    11041 aacctatact tggctcatct agtatataca aagaaccaac aaggctacta cctaaggaag
    11101 ttgccaaatt aatacgttga ctctcgccac ccgataaaga attcgataaa cgattcaggg
    11161 ttaggtattc cagaccaaca tcaatcaaat actgtaaacg attattaatt tcaataagta
    11221 agcgttttgc tatttgtttt tcttgttcag aaaattccgc gtttttaaaa aaatccaata
    11281 aagcataaat aggcatctca acaagttcac tgattgactt gccacacacc tttatataca
    11341 aggcttcctt ctttaattta gttccattac aggtcggaca aattgtttta cctctatatc
    11401 tgctaagtat taccctaaat tgaatcttat actgataaga ctcaagatac ttaaaaaatt
    11461 tatttaaacc tttgaaatac tcattcccgg tccatatcaa ttgtttttgc ttatctgtta
    11521 actcataata tggtttgtgc acaggaaaat caaatttttc tgcattatga atcaaatccc
    11581 acttatcatc ccccatcttt tcccctctcc aacaagcaat tgcatcctcg taaattgaca
    11641 aagatttatc aggaaccacc aaatcttcat ctatacctat tattttccca aatccctgac
    11701 aactaggaca tgctcctatc ggactattaa aactaaataa ttgctctgta ggctcttcaa
    11761 aacttatccc atccgcttcg aaattacgag aaaactcggt atctcctaca taacatattc
    11821 catgaccttc gtaaaaagca gtttcaacag aatcagccat tctatttata aaagactgat
    11881 ctttttgtac ggaaaaacgc tcaacaacta aaaataaacg ttcgtatgat ttatcgtaag
    11941 tagaaaattc gtcaattcta ataaattcat tattcacata aagacgacta aaaccctgca
    12001 tcttccaaat aggtatctga ttaacatcca aaacaggagc tgttaaaaca gtattttcgc
    12061 cttcaggcag cgatacaata taatccaata catcttttac ttgatgcttt ttaacctcca
    12121 caccggatat aggcgatata gtcttaccaa ttcgcgcata taacatacgc aaataatcat
    12181 atatctctgt tgatgtgcca accgttgagc gagaattccg agttttaacc ttttgttcaa
    12241 c
//
LOCUS       DAOPID010000017        12010 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_6331_length_12010_cov_4.676119, whole genome shotgun sequence.
ACCESSION   DAOPID010000017 DAOPID010000000
VERSION     DAOPID010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 12010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12010
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6331_length_12010_cov_4.676119"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1401)
                     /locus_tag="PK641_01360"
     CDS             complement(<1..1401)
                     /locus_tag="PK641_01360"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPG55036.1"
                     /translation="MKKLLISLIVTTLLVGCSSQGSGELVGVEGYSWKEPNPYGMVYI
                     KPGSFTMGANDQDANWAASTQAKVVSIDAFWMDETEITNREYKQFVYWVRDSIVREKL
                     INAEGDESEFVTKNKKTGDVVYKRGNRPNDKEKENVERLPKLNWDEKIPWTKPTEEQA
                     KAVDSMFYSDEESIAYTVELNGQILNYKYRWIDYEQAALKRNKFNSNKGRYESDLSML
                     GKGKDSADVMIKKDTSYIDENGHIINKTMFVELRGRRDFISSRIINVYPDTLCWIRDF
                     TYAYNEPYMKMYFYHDGYGDYPVVGVSWEQASAFCHWRTTYFNNAQIKSKQPIVQDYR
                     LPTESEWEYAARGGRNLSMYPWGGTYIRTAKGCFLGNFKPMHGNYVADGWMASAKVGS
                     FPPNDYGLYDMAGNVAEWTSSAFHESNYSFVNDMNPSYNYNARSSDPEIMKRKVIRGG
                     SWKDIGYYLQCATRTYE"
     gene            complement(1415..2350)
                     /locus_tag="PK641_01365"
     CDS             complement(1415..2350)
                     /locus_tag="PK641_01365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HPG55037.1"
                     /translation="MKKKISVIIAYFFCAISSFGQQNIEFSQYMLNLCTSNPAAVGNN
                     DMINVFGTFRSQHAGYEGAPLTYIVSADIGFNIGKTKHGAGISFYDNTAGLFRFQDVN
                     VQYAYRFPILDGYLSGGVIVDFTTVSIDKDKIHQAESEYHDAGDPAIDKAGNDFKMDL
                     GVGFLFKNKKWFAGISLLNVLSPEYELSEKTFFNKTMNLKFVGGYNISFNNPLYKLKT
                     SAMVNTDFVSWSGIINANLEYKDSYWGGIGYRIDGAVVFMAGIRVLNGLVIGYSFDLP
                     TSKLIQSAGSHEIVLSYSFNIDFLKKNRYKSIRYL"
     gene            complement(2467..2688)
                     /locus_tag="PK641_01370"
     CDS             complement(2467..2688)
                     /locus_tag="PK641_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HPG55038.1"
                     /translation="MYWTLELASKLEDAPWPATKDELIDFAVRSGAPLEVIENLQEIE
                     DEGEIYETIEDIWPDYPSKEDFFFNEDEY"
     gene            complement(2798..3301)
                     /locus_tag="PK641_01375"
     CDS             complement(2798..3301)
                     /locus_tag="PK641_01375"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPG55039.1"
                     /translation="MKVYTKTGDDGTSSLIGGRRVRKNDVHLEIYGTLDELSSFIGLL
                     KNQQELENYKNELTHIQQTLVNINCIFATDDPEIAKNNSFDIKNIQFLENKIDEYEQM
                     LPPVTDFLIPGENKSNAITNICRTICRRAERRINDLQLAEFQTAAAIYINRLSDYFFV
                     LGRKITY"
     gene            complement(3301..4086)
                     /locus_tag="PK641_01380"
     CDS             complement(3301..4086)
                     /locus_tag="PK641_01380"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPG55040.1"
                     /translation="MIKNKLNLIFRYIKYYFSALPKGGFGIHSPFVYSFVTEVIEESL
                     PYYSYNTIEHIRQNLLRDKSYINVTDYGTGKSCKKQICEIAKVSSKNKKDSQLIFRIA
                     VFAKAKNILELGTSIGTTTMYLGMSDHNSKVISLEGCPEIAKIAKTNLKNAKLSNVKI
                     ITGNIDNTLYDALKEMPTIDFAFFDANHQEVPTIKYFEQCLSKVDKNSIFVFDDIHWS
                     DGMDKAWKYICKNSKSVVTIDLFSMGIVFFNPELNKHNYKIKY"
     gene            complement(4083..6071)
                     /locus_tag="PK641_01385"
     CDS             complement(4083..6071)
                     /locus_tag="PK641_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HPG55041.1"
                     /translation="MENYRSLSAKEIETLISQSCTCDDWSRISVKERFSPCNIQHVHF
                     SGDIKLGVFDKEITLEGGIKKHTGIHHATIFNCNIHDNVFIGKINRYIANYEIDENTY
                     IENTELITTTGNTSFGNGTEVAILNEGGGREILITDKLSSHLAYIMALYRHREKTISA
                     LQNIIRQYSKDISSNVGYIGKNVRIISVGKIKDTKIGDYAYIEGASSLINGSINSNES
                     DPILVGQSVIAKNFIFSSGSQITDASLVENSFIGQGVQISKHFSIYDSVYFCNSQGFH
                     GEACAIFGGPFTVTHHKSSLLIAGMFSFLNAGSGSNQSNHMYKLGPIHQGVVERGSKT
                     TSDSYILWPAKVGAFSLVMGRHYQHSDTSNLPFSYLIEMDNSSYIVPGVNLRSVGTIR
                     DAQKWPKRDRRKDSEKLDQINFNLLSPYTIQKMYAGIDILKTLQKLSGETSETYSYQS
                     TFMKNQALRKGLIYYQMAIDKFLGNSLIKRLEGTSFKSINEIRERLKPDTSIGSGSWV
                     DISGLIAPKSEISQFMNDIESGSINNINSINERFKDIHSHYYSYEWTWALEKLEKSIN
                     KPYTEFSIQDIIKCVESWTKSVTDLDKLLYEDAKKEFDLISKTGFGADGDEEVKQKDF
                     ANVRGVFEENPFVMEVQNHIKIKTELGQELINRILPLA"
     gene            complement(6093..7298)
                     /gene="hflX"
                     /locus_tag="PK641_01390"
     CDS             complement(6093..7298)
                     /gene="hflX"
                     /locus_tag="PK641_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPG55042.1"
                     /translation="MTTDTDKDQEYAILVGLITPQQPERKAIEYLDELAFLADTAGAV
                     ISKKFFQRLDYANTKTFVGAGKLQEIQLYVNENNISLAIFDDELSPAQLRNLESELHI
                     KILDRTNLILDIFAQRAQTSHAKTQVELAQCRYMLPRLTRLWTHLDRQRGGIGMRGPG
                     ETQIETDRKILLNKISLLKQELVHIDKTKSVQRKNRGKLVRVALVGYTNVGKSTMMNL
                     LGKSDVLAENKLFATLDTTVRKVIIRNLPFLLSDTVGFIRKLPHDLIESFKSTLDEVR
                     EADILIHVVDISHSSFEEQIQVVEKTLHDIDCKDKPVIMVFNKIDSFEYQPKEEDDLT
                     PITRENISLDELRSTWMSKLKENCIFISAKNKTNIDELKQLLYEKVKVIHTTRFPYND
                     FLFENYEEQ"
     gene            complement(7373..8062)
                     /locus_tag="PK641_01395"
     CDS             complement(7373..8062)
                     /locus_tag="PK641_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPG55043.1"
                     /translation="MEIKKSPKADLENKKIISVLIGLVVALGILFVAFEWSQSDVTVY
                     EEALQGAAEVDEELVDITFRSETPPPPPPPQEQETVLSDVIEIKENTETVETADFSTE
                     DQSDKRVEIQAPIAAPEEEPEDQKKIHVIVEKMPEFKGGAEAMNNFLVRNIKYPLIAQ
                     ENNIQGRVICQFVVNSDGRIVDVEVVRGVEASLDAEAVRVIKSMPPWTPGKQGGKNVR
                     VKYTLPIRFKL"
     gene            8217..9248
                     /gene="porQ"
                     /locus_tag="PK641_01400"
     CDS             8217..9248
                     /gene="porQ"
                     /locus_tag="PK641_01400"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HPG55044.1"
                     /translation="MIERIRYILIFVLVSAAHMSAQQMGTAVNTFMDFPVSAHVAALG
                     GNNVSYIGKDAMFAVSNPALLSVNTDNLLYLNYAVYMVGTGYGSAAYSMKFSDKDAFS
                     VGFQFAQYGKMEGYDETETYIGNFSANDFALNATYSRYLNKYFTIGVTAKPVLNTYER
                     YTSFSLGVDIGVQFVDTASMVSLGLAVRNFGGQIAGPEDITMTSKWMPINVTLGLTKR
                     FKHAPFALHLTLQNLQKWDYDYATNSYEENAGKVNAGLMIGKKFIVGVDIIPRSEKFW
                     IGISYNFDRGLSLGNPYVFSLSGLSAGAGLRLYMFQIGVGVAFYSTAAVTGHFSVAMD
                     INGFNKKKL"
     gene            9250..9924
                     /gene="cmk"
                     /locus_tag="PK641_01405"
     CDS             9250..9924
                     /gene="cmk"
                     /locus_tag="PK641_01405"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HPG55045.1"
                     /translation="MKRITIAVDGYSSCGKSTMAKYMAKTLAYTYVDTGAMYRSVALY
                     AIRNKLINDSIVNEDQLRADISKIELSFDTRGNVLLNGEDVSNEIRSLEVSRFVSHIS
                     ALPFVRSRMVEIQRKMGKNGGVVMDGRDIGTVVFPKAELKIFVTADAGVRAKRRFDEM
                     RSNGKLESYEDILENIKQRDYMDEHRQESPLRKAEDAIVLDNSNMSIEEQNRWLMSRY
                     AEKCKN"
     gene            9902..10771
                     /locus_tag="PK641_01410"
     CDS             9902..10771
                     /locus_tag="PK641_01410"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HPG55046.1"
                     /translation="MQKNVKIEIDEDSGFCFGVVTAIGKAESELNGGNELYCLGDIVH
                     NGEEINRLAKMGLQVIGHSDLSHLKNAKVLLRAHGEPPSTYKTASDNGIKVIDATCPV
                     VLRLQQKIRKAYEEHPDSEIVIYGKLGHAEVNGLVGQTKGTAKVVESLSDIDLIDYSK
                     DILLFSQTTKSIDGYAALIEEIKKRKLKDTEFTAYDTICRQVSNRMERIRQFAKSHDL
                     VLFVGGEKSSNAKVLFKVCKQENDNSFFVSSSNDVQKEWLTDNVETIGICGATSTPNW
                     QMEEIKRKLLTII"
     gene            10774..11754
                     /gene="pfkA"
                     /locus_tag="PK641_01415"
     CDS             10774..11754
                     /gene="pfkA"
                     /locus_tag="PK641_01415"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPG55047.1"
                     /translation="MYQLKTIGVLTSGGDAPGMNAALRAVTRAAIFNGISVKAICRGY
                     KGIINNEIIDFKTQNVSNIIQHGGTIIKTARCQEFKTPEGRKLAYDTLKSHNIDALVV
                     LGGDGTFTGAKVFAQEYNFPIIGVPCTIDNDLYGTDFTIGYDTALNTVIEAVDKIRDT
                     ASSHDRLFFVEVMGRDAGFLALNAAIASGAEAAVIPEIAIKDDLLGEVIQNGFRKTKN
                     SSIVLVAESEITGGANGVAERVKREYPQYDVRVVILGHIQRGGSPSASDRILASRMGA
                     AAVDALIEEQRNLMIGIVNDAIVYVPFTKAIKDDKPIDLKLLQVLRSLSI"
     gene            11751..>12010
                     /locus_tag="PK641_01420"
     CDS             11751..>12010
                     /locus_tag="PK641_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55048.1"
                     /translation="MKTNSIYIKGARVNNLKNIDVEIPRGKLVVITGLSGSGKSSLAF
                     DTLYAEGQRRFVESLSSYSRQFLGRLSKPEVDFIQGLPPAIAV"
BASE COUNT         3571 a         2025 c         2167 g         4247 t
ORIGIN      
        1 ttcgtaagta cgggttgcac attgtaaata gtaacctatg tctttccatg aaccgccacg
       61 aataacttta cgtttcatga tttccggatc agaagatctt gcattgtaat tgtagcttgg
      121 gttcatatca ttaacgaaac tataatttga ttcatgaaat gcagatgatg tccattctgc
      181 aacgtttcca gccatgtcgt acaatccgta atcgttagga gggaatgatc cgactttagc
      241 tgaagccatc catccgtcag caacgtaatt accatgcatt ggcttgaagt ttcctaagaa
      301 acagcctttt gccgtacgta tataagttcc accccaagga tacatagata agtttctacc
      361 acctcttgct gcatattccc actcagattc ggtcggaagt ctgtaatctt gtacaatagg
      421 ttgtttgctt tttatttgtg cattgttgaa ataagttgtt ctccaatggc aaaatgctga
      481 tgcttgctcc cagctaacgc caactacagg atagtcacca tatccatcat ggtagaaata
      541 cattttcatg tatggttcat tgtatgcata agtgaaatcg cgtatccaac atagtgtatc
      601 aggatataca ttaattatgc gtgaagaaat aaagtcgcgt cttccgcgta attcaacaaa
      661 cattgtttta tttataatat gtccattctc atctatgtat gaagtgtctt tcttaatcat
      721 tacgtctgca ctgtctttgc ccttaccaag cattgataaa tcactttcgt agcgaccttt
      781 gttcgaatta aatttgttgc gttttaaagc tgcttgttca taatcaatcc agcgatactt
      841 gtaatttaga atttgtccgt ttaattcaac agtatatgcg attgattctt catcagagta
      901 gaacatagag tcgacagctt ttgcttgttc ttctgttggt tttgtccaag gaattttttc
      961 atcccagttt aacttaggaa gacgctccac attttctttt tctttgtcat ttggacgatt
     1021 accccttttg tagactacgt caccggtctt tttgttttta gtaacgaatt cggattcatc
     1081 tccttctgcg ttgattagtt tttcacgaac aatagagtca cgcacccaat aaacgaattg
     1141 tttatactct ctattggtta tttctgtttc atccatccag aatgcgtcga ttgaaacaac
     1201 ttttgcttgt gtagacgctg cccaattggc atcttggtcg tttgcaccca tagtaaacga
     1261 accgggtttg atgtaaacca tcccatacgg attaggctct ttccaagagt agccttcaac
     1321 acctaccaat tcaccacttc cttggctgct gcaacctact aacaatgtgg ttacaatcaa
     1381 agaaatcagt aattttttca tatttgtttt gcggttataa atatctgata cttttgtatc
     1441 tgtttttttt caagaaatca atattaaaac tgtacgatag tacaatttcg tgcgaacccg
     1501 ctgattgtat caactttgac gtaggcaaat caaacgagta tcctataacc aaaccattca
     1561 aaacccggat acctgccata aaaacaaccg ctccatcaat tctgtatccg attccacccc
     1621 aataactatc cttgtattct aaatttgcat tgataatacc tgaccaagaa acaaaatcgg
     1681 tattaaccat ggcggatgtc ttcaacttgt ataacggatt gttaaacgaa atattgtatc
     1741 cacctacaaa cttaaggttc attgttttgt taaaaaatgt tttctcactt aattcatatt
     1801 caggagataa aacattcaat aacgaaatac cagcaaacca tttcttattt ttaaacaaaa
     1861 atccaactcc caagtccatc ttaaaatcat tacctgcttt atctattgca gggtctcctg
     1921 catcatgata ttcactttct gcttgatgaa tcttgtcttt gtcaatactt accgttgtaa
     1981 aatcaactat tacgcctccg ctcaaatagc catctaatat tggaaatctg taggcatatt
     2041 gcacgtttac atcctgaaac cggaataaac ctgctgtgtt atcgtaaaac gatatacctg
     2101 cgccatgttt agtttttcca atgttaaaac cgatatctgc actaactata tatgtaagag
     2161 gagctccttc gtatccggca tgctgggatc taaatgttcc aaatacatta atcatatcat
     2221 tatttcccac cgctgcgggg tttgatgtgc ataaattcaa catatattga ctaaattcaa
     2281 tattctgctg accaaaggaa cttattgcgc aaaaaaaata cgctataata acggatatct
     2341 tctttttcat ataaaatctc acaagacaat agtctgtaag ttaaaatggt cgccgattat
     2401 taaacgtatc aacctccaat atcgtgcaaa tataataagt ttttatgaat tattagctct
     2461 tggctatcaa tattcatcct cattaaagaa aaaatcttct ttacttggat aatcaggcca
     2521 aatatcttct atagtttcat aaatctcacc ttcgtcttct atttcttgca aattttctat
     2581 tacttccaat ggtgcacctg aacgaactgc gaaatcaatt aattcgtctt ttgtagcggg
     2641 ccatggtgcg tcttccaatt tcgatgccaa ttctaatgtc caatacatga ttgtcgaatt
     2701 ttaatgtgaa caatgaaaaa aagcattatt ctgctatcag cgcacaaaga tagctaaatt
     2761 tttaatttca gcaagtgtag atgcgttttt tttgctttta gtatgtgatt tttctaccaa
     2821 gtacaaaaaa atagtcagac aaacgattga tatatattgc tgctgcggtt tggaattctg
     2881 ctaattggag gtcatttatt cttctttcag cgcgtctaca tattgttctg caaatgtttg
     2941 tgatagcatt ggatttattc tctcccggga tcaaaaaatc agtgacaggc ggaagcattt
     3001 gttcgtactc atcaatctta ttctctaaga attgaatgtt ttttatatca aaactattgt
     3061 tttttgcaat ttcggggtca tcagttgcaa aaatgcaatt aatattaact aacgtttgtt
     3121 gaatatgtgt aagttcgttt ttatagtttt cgagttcttg ttgatttttt agtaacccta
     3181 tgaacgaact taactcatct aaagtcccgt atatttcaag gtgaacatca ttctttctaa
     3241 cacgccttcc tccgattaaa ctgcttgtgc cgtcatctcc tgtttttgta tatactttca
     3301 ttagtactta attttgtagt tgtgtttgtt taattcagga ttaaaaaaga caatacccat
     3361 tgagaataaa tctattgtaa ctaccgattt agaatttttg caaatatact tccatgcttt
     3421 gtccattcca tcagaccaat gtatatcgtc aaatacgaat atactatttt tatcgacttt
     3481 tgataaacat tgttcaaaat attttattgt aggaacttcc tgatgatttg catcaaagaa
     3541 agcaaagtcg attgttggca tttcttttag cgcatcatat agagtattgt ctatatttcc
     3601 ggttataatt ttgacattgc ttaattttgc attttttagg ttagtctttg caatcttagc
     3661 tatttcaggg catccttcga gtgatataac cttactattg tgatctgaca tacctaaata
     3721 catagttgtt gtacctattg aagtgcccaa ttctaatata tttttagctt ttgcaaagac
     3781 tgctatacga aatatcagtt gtgaatcttt tttgtttttg gaactaactt ttgctatttc
     3841 acaaatttgc tttttgcatg atttgcctgt tccgtaatcc gtaacattaa tataagattt
     3901 gtcacgaagt aagttttgtc taatgtgctc aatagtatta tacgaataat aaggtaggga
     3961 ctcttcaata acctctgtta cgaacgaata tacaaaagga gagtgtatgc caaacccgcc
     4021 tttaggcaat gcggaaaagt aatattttat gtatctgaat attaagttca gcttgttttt
     4081 tatcatgcca aaggtaaaat tcgattaatt agttcttgac ccaattcagt ttttatttta
     4141 atatgattct gaacttccat tacaaatgga ttttcttcga acactccacg tacattagca
     4201 aaatcttttt gctttacttc ttcatcgccg tctgcaccaa aaccggtttt tgagataagg
     4261 tcgaattctt tttttgcatc ttcgtaaagt aatttatcta aatcggttac gctttttgtc
     4321 catgactcta cgcatttaat aatatcttga atagaaaatt cagtgtatgg cttatttata
     4381 gacttttcca atttttctaa agcccatgtc cattcataac tataatagtg tgaatgaatg
     4441 tctttaaaac gttcgtttat actgtttata ttattgatgc tgcctgattc tatatcattc
     4501 ataaattgag atatctcgct tttaggggct ataagtccgg aaatgtcaac ccatgagccg
     4561 ctacctattg atgtgtctgg ttttaacctt tcgcgtattt cattaataga tttgaatgag
     4621 gtgccttcta agcgttttat caatgaattt cctaagaatt tgtcaatagc catttgataa
     4681 tatatcaaac ccttacgcaa tgcttgattt ttcataaatg tgctttgata agaatatgtt
     4741 tctgatgttt caccggatag tttttgcaag gttttgagta tatcaatacc tgcatacatt
     4801 ttttgtattg tatatgggct tagtaggtta aagtttatct gatctaattt ttcagagtcc
     4861 tttctgcgat cacgctttgg ccatttttgt gcatcacgta tagtacctac acttcttagg
     4921 ttaacgcctg gaactatgta actgctattg tccatttcta ttaagtatga aaaaggtaga
     4981 ttggacgtat cagaatgctg gtagtgcctt cccataacta aagaaaatgc tccaactttt
     5041 gcaggccata aaatatatga gtcgctggta gtttttgatc ctcgttctac aactccttgg
     5101 tgtataggtc ctaacttgta catgtggtta ctttggttag atccactacc tgcatttagg
     5161 aaagaaaaca ttccggcgat tagtagactg gatttgtgat gggtaactgt aaatgggcct
     5221 ccaaatattg cgcatgcctc tccatgaaag ccttgactat tacaaaagta aacagaatcg
     5281 tatattgaaa agtgtttact tatttgaacg ccttgtccga taaatgaatt ttcaacaagt
     5341 gaagcatctg ttatttgtga tccggaagaa aatataaagt tcttggctat tacgctttgt
     5401 cccactaata taggatcaga ttcattacta tttatacttc cgttaattag tgaacttgcg
     5461 ccttctatat atgcgtaatc accgattttt gtatctttaa ttttaccaac actgataata
     5521 cgaacatttt tgcctatgta tccaacatta gaacttatat cttttgagta ttgacgaata
     5581 atattttgta aagcactaat agttttctct cgatgtctgt atagtgccat tatatatgct
     5641 agatgcgaac ttagcttatc tgtgattagg atttctctac caccaccttc atttaaaatg
     5701 gccacttctg taccgtttcc gaatgaggtg tttcctgttg ttgtaataag ctcagtgttt
     5761 tcgatgtaag tattctcgtc aatttcgtag ttggctatgt atctgttgat tttaccaata
     5821 aacacattat cgtgaatatt gcaattaaat atagtagcat ggtgtatgcc ggtatgcttt
     5881 tttatacctc cttcaagtgt aatttcttta tcaaaaacac ctaatttgat atctcctgaa
     5941 aagtgtacgt gttgaatatt gcaaggagaa aatctttctt ttactgagat tcgagaccaa
     6001 tcatcacaag tgcatgattg cgaaataagt gtctcgattt ctttagctga taacgatctg
     6061 taattttcca tctgccaagt gtgattaata gtttattgct cttcgtaatt ttcaaataaa
     6121 aaatcattgt aaggaaacct tgttgtatga atgactttta ctttttcgta caatagttgt
     6181 tttagctcgt caatgttggt tttgttttta gctgaaataa atatgcaatt ctctttcagt
     6241 ttagacatcc atgttgatct tagttcatcc aacgaaatat tttctcgagt tattggggtt
     6301 aaatcatctt cttcttttgg ttgatattcg aaagaatcaa ttttgttaaa gaccattatg
     6361 acaggcttat ccttacaatc aatgtcgtgc aatgtttttt caactacctg aatttgttct
     6421 tcaaaagatg aatgcgaaat atcaacaacg tgaattagaa tatctgcttc acgaacttca
     6481 tctaatgttg atttaaatga ttctattagg tcatgaggta atttacgaat aaatcccaca
     6541 gtatctgata gtagaaatgg aagatttctg atgattacct ttcttactgt agtatcaagt
     6601 gttgcaaata atttattttc tgcaagtacg tcggattttc ctagtaggtt catcattgtt
     6661 gattttccta catttgtgta tcctactaat gctacacgaa ctaattttcc tctattcttg
     6721 cgttgaacgc ttttcgtctt atcaatgtgt acaagttcct gctttagaag agatatcttg
     6781 tttagtaata tttttctatc agtttcgatt tgtgtttcac cgggccctct cattccaata
     6841 ccaccacgtt gtctgtcaag gtgtgtccat aaacgtgtaa gtcttggtaa catatatctg
     6901 cattgtgcaa gttctacttg agtttttgca tgtgatgttt gtgctctttg tgcaaaaata
     6961 tcaagtataa gatttgttct gtctaaaatc ttaatatgta attcggattc taagtttctg
     7021 agttgcgcag gagaaagttc gtcatcaaaa attgctaaac tgatgttgtt ctcatttaca
     7081 tataactgta tttcttgtag ttttccggca cctacaaatg tttttgtatt ggcgtaatct
     7141 aatctttgaa aaaacttttt agagatgact gcaccggctg tatctgccaa aaaagccaat
     7201 tcatcaaggt attcaatagc cttacgctct ggttgttgcg gggttattag acctacaagt
     7261 atggcatatt cttggtcttt gtcagtgtct gtagtcatta tgtttagttt gatgttttta
     7321 aaaaaagcct ccaaaatgaa ggctttttta gagaactatg tattatgcat ttttacaatt
     7381 taaatctaat cggtaaagtg tatttaacgc gaacattttt accaccttgt ttgccaggag
     7441 tccatggagg cattgatttg atgactctaa cagcttctgc atccaaactg gcttcaacac
     7501 cacgtacaac ttcaacatca actattctac catctgaatt aacaacgaat tggcatataa
     7561 cacgaccttg aatgttattt tcttgtgcta ttaatggata ttttatattc cttaccaaaa
     7621 agttattcat tgcttcagcg ccgcctttaa attcaggcat tttttcaact attacgtgaa
     7681 ttttcttttg atcttcaggc tcttcttcag gagccgcaat aggagcttga atctcaacac
     7741 gtttatctga ttgatcttcc gtagagaagt ctgcagtttc tactgtttct gtgttttctt
     7801 ttatttcgat aacatcactc agtactgtct cttgttcttg tggaggtgga ggaggtggag
     7861 gggtctcact acggaatgta atatcaacta attcttcgtc tacttcagca gcaccttgta
     7921 atgcttcttc gtatactgta acatcacttt gagaccattc gaaagcaacg aataagattc
     7981 ccaaagcaac aactaaacca attagaacag agattatttt tttgttctct aaatcggctt
     8041 tcggtgattt tttgatttcc atattatttt aaaaataatt gttgctaagt tgttcgcaaa
     8101 tgtagtgttt tttttttata tgcatagact ttatacgtat ttttttttat cagatatttt
     8161 gttgtgttac ttatgatggt tttgtgccaa aaaaaacgta tatttgggcg ttttttatga
     8221 ttgaaagaat tagatatata ttgatttttg ttcttgtttc ggctgcacat atgtcggccc
     8281 agcaaatggg aactgcggtg aatacattta tggatttccc ggtatctgca cacgttgctg
     8341 ctttaggtgg taataatgtt tcgtatattg gtaaagatgc aatgtttgct gtatcaaatc
     8401 cggctctgtt gtctgtaaat accgataatc ttctgtatct taattatgcc gtatatatgg
     8461 taggcacagg ttatggttct gcagcttatt cgatgaaatt tagtgataaa gatgccttct
     8521 ctgtaggatt ccaatttgca caatacggaa agatggaagg ttacgacgaa acagaaacct
     8581 atataggtaa tttctctgca aatgattttg ctttaaatgc gacatactca aggtatctta
     8641 ataagtattt caccattggt gtaacggcaa aacctgtttt aaatacttac gaaagatata
     8701 cttctttctc attaggagtt gatataggtg tccagtttgt agatacggca agcatggttt
     8761 cattaggttt agcagtgcga aattttggag gacaaatagc aggtcctgaa gatattacaa
     8821 tgacaagtaa atggatgcct attaatgtca cattaggtct tactaaaagg tttaagcatg
     8881 cgccattcgc tcttcatctg acattacaaa atttacagaa atgggattat gattatgcta
     8941 caaattctta tgaagagaat gcgggtaaag ttaatgcagg tttgatgata ggaaagaagt
     9001 ttattgtagg tgtggatatt atcccgcgct ctgaaaaatt ctggataggc atatcttata
     9061 attttgatcg tggtttaagt ttgggaaatc cttacgtctt ctcactctca ggtttatcgg
     9121 caggtgcggg tttacgccta tatatgttcc aaataggtgt aggtgttgca ttttatagca
     9181 cggcagctgt aacagggcat ttctctgttg ctatggatat taatggattc aataaaaaga
     9241 aattataata tgaagcgtat tactatagct gttgatggat attcttcttg tggaaagagt
     9301 actatggcta agtatatggc aaaaacttta gcttatacat atgttgatac gggtgctatg
     9361 tatcgatccg ttgctttata tgcaataaga aataaattga ttaatgactc tattgtaaat
     9421 gaagatcagt tacgtgcaga tatatctaaa atagaattga gttttgatac aagaggaaat
     9481 gtgttgttaa atggtgaaga tgtgtcgaat gaaattcgca gtttagaggt ttctcgtttt
     9541 gtaagtcata ttagtgcatt gccatttgtt cgttcaagaa tggttgaaat acagcgtaaa
     9601 atgggtaaaa acggtggtgt ggttatggat ggtagagata taggtactgt tgtatttcca
     9661 aaagccgagt taaaaatatt tgtaactgct gatgccggtg tgcgtgcaaa gagacgtttc
     9721 gacgaaatga ggtctaatgg taaacttgaa tcatatgaag atatacttga aaatattaag
     9781 caacgtgatt acatggatga gcatagacaa gaaagccctc ttagaaaagc tgaggatgcg
     9841 atagtgctcg ataatagtaa tatgagtatt gaagaacaga atagatggtt aatgtctcga
     9901 tatgcagaaa aatgtaaaaa ttgaaataga tgaagattcc ggtttttgtt ttggagtagt
     9961 aacagctatt ggtaaggccg aatctgaatt gaatggtgga aatgaattgt attgtttagg
    10021 cgacatagtt cataatggag aagagataaa tcgtttggct aaaatgggtt tgcaggtaat
    10081 tggtcattcc gatttatcgc atcttaaaaa tgcaaaagtt ttacttaggg cacatggtga
    10141 gccaccgtca acctataaaa cagcgtcgga taatggaatt aaagttatag atgctacatg
    10201 tcctgtggtt ttgcgtttgc aacagaaaat tcgtaaggct tacgaggagc atcctgatag
    10261 tgaaattgtt atatatggta aattaggtca tgcagaggtt aatggactgg taggtcaaac
    10321 taaaggaaca gcgaaagttg tagaatcttt atctgatatt gatttgattg attatagcaa
    10381 ggatatattg ttattttccc aaactactaa atcaatagac ggatatgctg ctttaataga
    10441 ggaaattaaa aaacgtaaat taaaggacac agaatttacg gcttatgata caatttgtag
    10501 gcaggtgtct aatagaatgg agagaataag acaatttgct aaatctcacg atttagtttt
    10561 gtttgttgga ggggaaaaga gttctaacgc caaggtatta ttcaaagtgt gcaaacaaga
    10621 aaatgacaac agtttttttg tttcgtcgtc aaatgatgta caaaaagaat ggttaacaga
    10681 taatgtggaa actataggaa tctgtggtgc aacctcaact cctaattggc aaatggaaga
    10741 aattaaacgt aaacttttaa ctataatata atcatgtatc aattaaaaac aataggtgta
    10801 cttacttcag gtggtgatgc accaggtatg aatgctgcat tacgtgcggt gactcgtgcg
    10861 gcaattttta acggaatatc tgtaaaagca atatgcagag gttacaaagg cattattaat
    10921 aatgaaataa ttgattttaa aactcaaaat gttagtaata tcatacaaca tggaggaact
    10981 ataattaaaa ctgcaagatg ccaagaattt aaaactcctg aaggtagaaa attagcatac
    11041 gacactttaa aatcgcacaa catagatgct ttagttgtat taggtggtga cggtactttt
    11101 accggtgcaa aagtgtttgc tcaagagtat aattttccaa taataggcgt accatgtact
    11161 atcgataatg atttatatgg aactgacttt acaataggtt atgacacagc acttaacaca
    11221 gttattgagg ctgttgataa gattcgtgat acggcatctt ctcacgatcg tttattcttt
    11281 gtcgaagtaa tgggtcgcga tgccggtttc ttagctttaa atgctgcaat tgcatcaggt
    11341 gctgaagctg ctgttatacc tgaaatagca atcaaagatg atttgctcgg tgaagttatt
    11401 caaaatggtt ttagaaaaac caagaatagt agtattgtac tcgttgctga aagtgaaatt
    11461 accggaggtg caaatggtgt tgcagagcgt gtaaaacgtg aatatccgca atatgatgta
    11521 agagttgtaa ttttaggaca catacaacgt ggtggttcgc cgtctgcatc agatcgtata
    11581 ttggctagcc gaatgggtgc tgccgctgtg gatgctttaa ttgaagaaca acgcaactta
    11641 atgataggta tagttaatga tgcaattgtt tatgtgccgt ttacaaaagc tataaaggat
    11701 gataagccta ttgatttaaa attattgcag gttcttaggt ctttgtcgat ataaaaacta
    11761 attctattta tattaaaggg gcacgtgtca acaatctaaa aaatatagat gttgaaatac
    11821 cacgtggaaa attggtcgtc attacaggat tatcaggctc aggtaaatca tctcttgctt
    11881 ttgatacttt gtatgctgag ggtcagaggc gttttgtcga aagtttatca tcatattcac
    11941 gtcaattttt aggtaggcta agtaaacctg aagtcgactt tattcaaggt ttacctcctg
    12001 caatagctgt
//
LOCUS       DAOPID010000018        11994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_6344_length_11994_cov_4.576681, whole genome shotgun sequence.
ACCESSION   DAOPID010000018 DAOPID010000000
VERSION     DAOPID010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 11994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11994
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6344_length_11994_cov_4.576681"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="PK641_01425"
     CDS             <1..914
                     /locus_tag="PK641_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HPG55049.1"
                     /translation="DVNSIKRSDLNILIKVTEALHEKKIVQIADKIAHNQNIRVILVA
                     GPSSSGKTTFSKRLSVQLAASGIIPEALSLDNYFINRESTPIDENGEHDFESIHALDL
                     PYLNSQLEQLINGKEVETPIYNFETGKREAKGKKMQIKDGQVLVIEGIHGLNPELTKT
                     IPDETKFKIYVSALTTLSGNDHNWIPTSDTRILRRIIRDHKYRSYSAENTILRWPSVR
                     RGENKWIFPYQENADAMFNSSLLFELSAIKKQAEPILMEVGQNSNAYPEARRLLNLLQ
                     FFTPISYNDIPATSLLREFLGGSGFRY"
     gene            complement(919..1815)
                     /locus_tag="PK641_01430"
     CDS             complement(919..1815)
                     /locus_tag="PK641_01430"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPG55050.1"
                     /translation="MWVLLTLCSAFCLGIYDVLRKYVLRYNAVLPILLCSTFTSSIFM
                     AVFLILSKVGIIPMDSYYFVPSIPLYMHTMLVVKAAIVLSSWICVYYALKNLPLSIVS
                     PIRSTAPMWTLIGALVIYSERLNLWQWSGFLVVMAAFMLFAIEGKKEGIRFVANIWVW
                     LVLIGTLIGSASALFDKYLVRELNIPRMAILTYYSFYEVILLIPLLLIIWFPHRKENP
                     WVWMWAAPFIGIVIVLADFFYYGAIQCPDSLISLISPIRRSNVLVSFTFAAFLFKEKN
                     ILRKSICLAGILLGVALIAVGS"
     gene            complement(1821..3305)
                     /locus_tag="PK641_01435"
     CDS             complement(1821..3305)
                     /locus_tag="PK641_01435"
                     /inference="COORDINATES: protein motif:HMM:NF010184.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HPG55051.1"
                     /translation="MQKLGFNNQMYLQTQSHKIAERIAFFGGKLYLEFGGKLFDDFHA
                     SRVLPGFEPDSKISMLVNLKEDAEIIIVINSADIEKNKLREDIGITYDVDVLRLIDAF
                     RGYELYVSSVVLTQFEDQPAVVAYQKKLEALGLKVYRHYPIQGYPSNISFIVSDEGYG
                     KNEYIETSKSLVVVTAPGPGSGKMATCLSQLYHDNKRGIKAGYAKFETFPIWNLPLKH
                     PVNMAYEAATADLNDVNMIDPFHLEAYGETAVNYNRDVEIFPVLTAMFERIVGSCPYK
                     SPTDMGVNMAGNCIIDDEVVSIAAKNEIVRRYYRTLCSVRNGNASIDQVYKLKLLMEK
                     AKVSISDRRVVAVAVSKAEETANPAAAIELNDNQIVTGKTGDLLGASSAMLLNALKNL
                     AGIPDEIQLISPSVIAPIQDLKIAHLGNLNARLHTDEVLIALSICALNDINAEKAINC
                     LHLLRGCEVHSSVILSQVDVSVFRSLGINLTCEPTYQTNKLYHR"
     gene            complement(3333..4862)
                     /gene="ispG"
                     /locus_tag="PK641_01440"
     CDS             complement(3333..4862)
                     /gene="ispG"
                     /locus_tag="PK641_01440"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HPG55052.1"
                     /translation="MINYIRRKSSVVKVGNVCIGGNNPIRIQSMLNTSTMDTEASVNQ
                     TVDLIKAGSELVRLTTQGEREAKNMALIREELNKRSYNTPLVADVHFNSKVADEAALH
                     VEKVRINPGNYANSIDEIEHKFVPFLNLCKQHNTAIRLGVNHGSLNKRILEKYGDTPE
                     GMTESCMEFLRICKRENFSNVIISVKASNTRVMVQTVRQMARRMQEEGMNFPLHLGVT
                     EAGSGEDGRIKSAVGIGTLLSDGLGDTIRVSLSESPVAEIPVAKKIVDFISRKSKAAI
                     IAGFDENLYNPEQYSRRETLAVGSFGGNQVPLVLTAEETLPNGVLDCVLSSDNALGEG
                     RLFFANLKSKNNKQPVVLTRVYNTSDLETLQIEASIELGPFFIDGLGDGIRIVAPNIA
                     ADIVNSLSYGILQATGARITKTEYISCPSCGRTLFDIESTIEKIKAATSHLKSLKIGI
                     MGCVVNGPGEMADADYGYVGAGVGRVSLYRGQQCVEKNIPEEQAVEHLIALIRSDGRW
                     V"
     gene            complement(4872..6155)
                     /gene="murF"
                     /locus_tag="PK641_01445"
     CDS             complement(4872..6155)
                     /gene="murF"
                     /locus_tag="PK641_01445"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008662826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HPG55053.1"
                     /translation="MEIEQLYECFERCKQVSTDSRNCPKGSLFFAMRGANFNGNAYVS
                     KAFEQGCAMAVIDDKDYFSDERCILVEDCLKTLQDLARFHREKLGTPIIGITGTNGKT
                     TTKELVSAVLSQKYNILYTQGNLNNHIGVPLTLLRLTNEHDMAVVEMGANHPGEIESL
                     VNISKPNYGLITNVGKAHLEGFGSFEGVMKTKGELYEYLRMSKGKVFLHADNPYLNKM
                     ATDLDTITYGISDSNYVSGSLIGVTPYLKVQYNTINGSIEINTNLIGDYNLANVMSAV
                     CVGRYFNVSDDKIKIAIESYFPTNNRSQLCKTNDNTLIIDAYNANPTSMDAALQNFAK
                     IDATSKCVVLGDMFELGETSLTEHQNIVHLIEKIGVHDVYLLGKNFSATKNEFKCFEN
                     TEAFANYLKENPIIGKTILIKGSNSMKMSSLVSYL"
     gene            6241..7575
                     /locus_tag="PK641_01450"
     CDS             6241..7575
                     /locus_tag="PK641_01450"
                     /inference="COORDINATES: protein motif:HMM:NF039698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6242 domain-containing protein"
                     /protein_id="HPG55054.1"
                     /translation="MTNFDQNYTPVMHKRVPLLCGLSILISTIFSACLTEPSTSQEAH
                     IQSFYFNANDSAPDISSITFTVDTTNLIIFNTDSVAYDSNISQIVPVIVGYGSFSGIT
                     VNSITWNQTDSLDFRNPVSITVTAADKKTSHTYNVVVNKHKVNPDSIVWTKTVDAIAQ
                     IDNIKATQAFYVENKLFCAIQSENRSDIYVADTETNWSSDINSTIEFNVRSITVREND
                     IVMLTNDGSAMYSWNPTTKALEQLANAPEGYEIKDLIGYIDDEIWAISINNGIKTILS
                     YSNNEWNNISDASIEAEGFPVEGSAKMQWNNTMPYLLGGSNASGVLQNCVFSTENGKY
                     WTNLIKSPNYGFGNRKDAAAIYLDNKIFIFGGSNGSTIVSTTYLSHDNGFSWSNALEY
                     QELPTTYTDKTGISAVVDNQNYIWLIGGKDENGSFHTDVWKGRINKFDFLLQ"
     gene            7581..8024
                     /gene="coaD"
                     /locus_tag="PK641_01455"
     CDS             7581..8024
                     /gene="coaD"
                     /locus_tag="PK641_01455"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HPG55055.1"
                     /translation="MERIALFPGSFNPFTKGHESIVSRSLSIFDKIIIAIGVNGDKNI
                     EFIDKRMDSIRQLYIDNPRVIVVNYETLTADVVKKYNANCIIRGIRNGKDLEYETNIA
                     EVNYSFFGVETIFMLSEPNLKKVSSSIVRELQKYGKDVSSYLPNK"
     gene            complement(8028..8486)
                     /gene="rpiB"
                     /locus_tag="PK641_01460"
     CDS             complement(8028..8486)
                     /gene="rpiB"
                     /locus_tag="PK641_01460"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HPG55056.1"
                     /translation="MKDLDTLSVAIACDHAGYELKEEIKQYFGAKLFELKDFGTFSAD
                     SVDYPDMINPMAAEINAGKFDFGIAICGTGNGVSMVANKYRNVRAALCWKNEIALLAR
                     KHNDANIISLPARFISLAEAEIMIESFLTTDFEGGRHSARVNKISNANNL"
     gene            complement(8614..9837)
                     /locus_tag="PK641_01465"
     CDS             complement(8614..9837)
                     /locus_tag="PK641_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HPG55057.1"
                     /translation="MKSNCIILSKKISGSVILPASKSISNRALILKALSGSSDVKNLS
                     CCDDTDAVVAALSSFKNEINVGAAGTAMRFLTAYLSLKAGEWQITGSERMLHRPIKIL
                     VDALRNIGADIEYLNEEGFPPLRIKGHSLIGGDIEIDGSVSSQYISALLMIAPYTQNG
                     INMYLNGKISSKPYIKMTLSMMESFGVSTVWHDNVIKIFPQKYSNTSYIVENDWSAAS
                     YWYEIISLTENAELELIGLSKNSLQGDSVVATIFESLGVQTEYLNGNVRIKKTSTPVK
                     KLFYDFNSCPDLAQTIVVTCCLLGVQFNFCGLQSLKIKETDRIAALKKECAKMGFKLE
                     EIGIGELSWNGLKIETSGEFSIDTYDDHRMAMAFAPAAMCLQNILINNPNVVSKSYPL
                     FWEDLKEHGFLVLSQ"
     gene            10043..11650
                     /gene="pckA"
                     /locus_tag="PK641_01470"
     CDS             10043..11650
                     /gene="pckA"
                     /locus_tag="PK641_01470"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HPG55058.1"
                     /translation="MANLDLSKYGINGAIEVLHNPSFDVLYAEETKPGLEGFEKGQVT
                     ELGAVNVMTGVYTGRSPKDKFFVKNEASEDSLWWTSDEYKNDNKPCSEAAWADLKAKA
                     VKQLSGKRLFVIDTFCGANPATRLKVRFIMEVAWQAHFVANMFIRPTDQELANYGEPD
                     FVSFNAAKAKVENFKELGLNSETATVFNLKTKEQVILNTWYGGEMKKGIFSIMNYLNP
                     LKGIASMHCSANTDKAGTSTAIFFGLSGTGKTTLSTDPKRLLIGDDEHGWDNEGVFNY
                     EGGCYAKVINLDKDSEPDIYKAIRRDALLENVTVDANGKIDFNDKSVTENTRVSYPIE
                     HIENIVKPVSKGPHAKQVIFLSADAFGVLPPVSILTPEQTKYYFLSGFTAKLAGTERG
                     ITEPTPTFSACFGAAFLSLHPTKYGEELVKKMEMTGAKAYLVNTGWNGTGKRISIKDT
                     RGIIDAILDGSIDKAPTKQIPMFDFKVPTALPNVDPKILDPRDTYASASQWDEKAKDL
                     AARFIKNFAKFEGNEAGKALVAAGPKL"
     gene            11809..>11994
                     /locus_tag="PK641_01475"
     CDS             11809..>11994
                     /locus_tag="PK641_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55059.1"
                     /translation="MIEDTSLILQDLKFKNTYTDDSIKTLEGLEHIRLRPGMYIGKLG
                     NGEYADDGIYVLLKEVVD"
BASE COUNT         4092 a         2281 c         1911 g         3710 t
ORIGIN      
        1 cagatgtaaa tagtattaaa cgaagtgatc ttaatattct tattaaggta acagaggctc
       61 ttcacgaaaa gaagatagtt caaatagcgg acaaaatagc acataatcaa aacataagag
      121 tgatactggt tgcaggtcca tcatcttcag ggaaaacaac attcagtaaa cgtctaagcg
      181 tacaattagc agcatcaggg atcattccgg aagctctatc tttagataac tatttcatca
      241 accgcgaatc aactccaata gacgaaaacg gagaacatga ttttgaatct atccatgctc
      301 ttgatcttcc gtaccttaat agccaattgg agcaactaat aaatgggaaa gaagttgaga
      361 ctcccatata caactttgaa acaggaaagc gggaagcaaa aggcaaaaag atgcaaataa
      421 aagacggaca agttttagtc attgagggca tccatggttt aaatccggaa ctaaccaaaa
      481 caatacctga tgagacaaaa tttaaaatat atgtttctgc attaacaact ctttccggca
      541 atgaccacaa ttggattcct actagtgaca ctagaatatt gcgacgcatc attagagatc
      601 acaagtacag aagttattcg gcagaaaata ccattttaag atggccaagt gtcagacgtg
      661 gagagaataa gtggattttc ccctaccaag agaatgctga tgcaatgttt aactcatcat
      721 tattatttga gttgtctgca ataaagaaac aagctgagcc aatacttatg gaagtaggac
      781 aaaactctaa tgcatatcct gaagccagac gtttattaaa tcttttacaa ttttttactc
      841 ctatttctta caatgatatt cctgcaacat cactactaag agaattttta ggaggtagcg
      901 gatttagata ttaatgaatc aactgcctac agcaatcaag gcaacaccaa gtagaatacc
      961 tgcaaggcaa atagacttgc ggagaatgtt cttttcttta aataaaaagg ctgcaaatgt
     1021 aaatgatact aaaacattgc tacgccttat tggtgatatt aatgatatta agctatcagg
     1081 acactgaatc gccccataat agaagaaatc agccaataca ataacaattc ctataaaagg
     1141 agctgcccac atccataccc acggattttc tttacgatga ggaaaccata ttattaataa
     1201 caatggaatt aataatatga cctcatagaa agaatagtag gttagaatcg ccattctggg
     1261 gatatttagt tccctaacta aatacttatc aaatagtgca cttgcagaac ctataagagt
     1321 cccaattaaa actaaccaaa cccatatatt tgcgacaaaa cgtataccct cttttttacc
     1381 ttctatagcg aataacataa acgctgccat taccacaaga aatcctgacc actgccataa
     1441 attcaatctt tccgaataga ttaccaatgc tccaataaga gtccacatag gagcagtcga
     1501 cctaattgga gaaacaatac ttaatggtaa attctttaat gcataataaa cacaaatcca
     1561 tgaagacaat actatcgcag cttttacaac aagcattgta tgcatgtaca aaggtatact
     1621 aggaacaaaa taatacgaat ccatcggtat aataccaacc tttgacagaa ttaaaaaaac
     1681 tgccataaaa atagacgaag taaacgtgct gcacaacaat ataggcaaaa cggcattata
     1741 acgtaacaca tatttgcgta acacgtcata tatgcctaaa caaaaagcag agcaaagggt
     1801 taataatacc cacataatac ctaacgatgg tataatttgt ttgtttgata agtaggttcg
     1861 caggtaagat taatacctaa actacgaaat acacttacat caacttgcga taagataaca
     1921 ctagaatgaa cttcacatcc ccttaacaaa tgcaaacaat taatcgcttt ttcagcattt
     1981 atatcgttta atgcacatat tgataaagca attagtacct cgtcagtatg aagcctcgca
     2041 tttaagtttc ccaaatgcgc tatctttaag tcttgtatag gtgctatgac agatggtgaa
     2101 ataagttgaa tttcatctgg aattccagcc aggttcttaa gcgcattaag cagcattgca
     2161 gaagatgctc caagtaaatc acctgtcttt cctgttacaa tctgattatc atttaattca
     2221 atagcagctg caggattagc agtttcttct gctttagaaa cagcaactgc tactactctt
     2281 ctatcagata tactaacttt tgccttttcc atcaacaact ttaatttgta tacctgatct
     2341 atagatgcat taccatttct tacactacat agtgttctgt aatatcttct aactatttca
     2401 tttttagcag caattgacac aacctcatcg tcaattatac aatttccggc catgtttaca
     2461 cccatatctg ttggagattt atatgggcaa ctgcccacta tacgttcaaa cattgccgtt
     2521 aatacaggaa atatttcaac atcacgattg taattaacag ctgtttctcc gtatgcctcc
     2581 aaatggaatg gatcaatcat gtttacatca ttcaagtctg cagttgcagc ttcataagcc
     2641 atatttacag gatgctttaa tggcaaattc caaattggga atgtctcaaa ttttgcatat
     2701 cctgctttaa ttcctctttt attatcatga tacaattgag ataaacaagt tgccatttta
     2761 ccacttccgg gacctggggc agtaacaact accaaagatt tggaagtctc aatatattcg
     2821 ttttttccat aaccttcatc actaacaata aaagagatat tagaaggata tccttgaata
     2881 ggatagtggc gataaacttt taaacctaat gcttcaagct ttttctgata ggccacaaca
     2941 gcaggctgat cttcaaattg agtcaatacc acactactta catatagttc atatccgcgg
     3001 aaagcatcaa ttaatctaag aacatcaaca tcgtaagtaa tgcctatatc ttctcttaat
     3061 ttattttttt cgatatctgc tgaattgatt acaataataa tctcagcatc ctcctttaaa
     3121 tttaccaaca tactaatctt gctatccggt tcgaatccgg gcaaaacacg tgaggcatga
     3181 aaatcatcaa ataattttcc accaaattcc aaatacaatt tacctccgaa aaaagctatg
     3241 cgctctgcaa ttttgtgaga ttgcgtttga aggtacattt ggttgttaaa tccgagcttt
     3301 tgcataataa tagtatatct tttttatcaa atttaaaccc atctaccatc tgagcgtatc
     3361 agagcaataa gatgctctac cgcttgttct tcgggaatat tcttctctac acattgttgt
     3421 cccctataaa gacttacacg accgaccccg gctcctacgt atccataatc agcatccgcc
     3481 atttcgccgg gtccgttaac aacacaaccc ataataccaa tcttaagtga ttttaaatga
     3541 gatgtagctg ccttaatttt ttcaattgta gattcaatat caaataaagt tctaccgcaa
     3601 cttgggcacg aaatgtattc agtcttagta atacgcgcac ctgttgcttg cagtattcca
     3661 taagataaag agtttacaat atccgctgct atgtttggtg ccacaattct tattccatct
     3721 cctaaaccat caataaaaaa tggtccaagt tcgatggaag cctctatttg caaagtttct
     3781 aaatcagaag tattatacac ccttgttaac acaacaggtt gtttgttatt cttagatttt
     3841 agatttgcaa agaacaaacg accttcaccc aaagcattat cacttgacaa cacgcaatca
     3901 agtactccat taggcaaagt ttcttctgca gtaagtacca atgggacttg attcccccca
     3961 aaagaaccaa cagccaaagt ttctctccga ctatattgct cgggattata taaattttca
     4021 tcaaaacctg cgattatggc agctttgctc tttctactga tgaaatcgac tattttttta
     4081 gctaccggaa tttctgcaac cggtgactca cttaaagata ccctgatcgt atcacctaag
     4141 ccgtcagaca ataacgttcc tattccaacc gcagatttaa tacggccgtc ttctccactt
     4201 cctgcttcag taactccaag atgtagtggg aaattcattc cttcttcctg cattcttctt
     4261 gccatctgtc gcacagtttg gaccattaca cgagtattag atgccttgac tgaaataatt
     4321 acattactaa aattttctct cttacatata cgtaaaaact ccatacaaga ttcagtcata
     4381 ccttcgggag tatcaccata tttttctaga attcttttat taagtgaacc atgattaact
     4441 cctaatctaa tagctgtatt atgttgtttg cataaattca gaaatggaac aaacttatgc
     4501 tctatttcat cgattgaatt agcataattg ccgggattaa ttcttacttt ttcaacgtga
     4561 agcgcagctt catctgcaac ttttgaatta aaatgtacat cagcaaccaa aggcgtgtta
     4621 taagaacgtt tattaagttc ttcgcgtatt aaagccatgt ttttggcttc gcgttcacct
     4681 tgtgttgtta atcgtactaa ttcactgccc gcttttatta aatcgacagt ctgatttaca
     4741 gaggcttcag tatccatagt agaagtattc agcatagact gaatgcgtat aggattattt
     4801 cctcctatac atacattgcc gacttttact acactcgact ttctgcgaat ataattaatc
     4861 atactttttt tttataaata tgacaccaag ctactcattt tcattgagtt cgagcccttt
     4921 atcaatattg ttttacctat tattggattt tcttttaagt aattcgcaaa tgcttctgta
     4981 ttctcaaaac acttaaattc gttctttgtt gcagaaaaat tcttacctaa aagatataca
     5041 tcatgaacgc ctattttctc gattaaatgg actatatttt gatgttccgt cagactagtt
     5101 tctcccaatt caaacatatc ccccaacact acacatttag aagttgcatc aatcttagca
     5161 aaattctgca atgctgcatc catgcttgta ggattggcat tgtaggcatc tattataagc
     5221 gtgttatcat ttgttttaca aagttgagaa cgattatttg taggaaaata ggattctatg
     5281 gcaatcttaa tcttatcatc agaaacatta aaataacgac ctacacatac agcagacata
     5341 acatttgcca gattataatc gcctattaaa ttagtattaa tttctattga gccattaatc
     5401 gtattgtatt gtaccttcag ataaggagtt acgccaataa gtgatcctga aacataatta
     5461 gaatcagata ttccgtatgt aatagtatcc aaatctgttg ccatcttgtt aagatatgga
     5521 ttatctgcat gcaaaaacac ctttccctta ctcattctca ggtattcata taattcgcct
     5581 tttgttttca taaccccctc aaaagagcca aagccttcta aatgagcctt tccaacatta
     5641 gttatcagtc cgtaattagg cttcgatata ttaaccaatg actcaatttc accagggtga
     5701 ttagcgccca tttctaccac agccatatca tgttcattag ttaaacgaag taaagtaaga
     5761 ggtacaccaa tatgattatt taaatttcct tgagtatata atatgttata tttttgagac
     5821 aatacggcag acaccaattc tttagtggta gtcttgccat tggtacctgt aataccaatt
     5881 attggagttc ctaatttttc ccgatgaaat cgagctaaat cttgtaatgt ttttaagcaa
     5941 tcttctacta gtatacaacg ttcatcgcta aagtaatctt tgtcatcaat cacagccatt
     6001 gcacagcctt gctcaaatgc ctttgacaca taagcattac cattaaaatt agcacctctc
     6061 attgcaaaaa atagcgagcc cttagggcag tttcggctat cagtcgaaac ttgcttgcaa
     6121 cgttcgaaac actcatataa ctgctcgatt tccattttat cgttattttt gacaaaagta
     6181 ctgcaatatt tctaactttg acaatccttg aggtttttta tttttagaat aaaaaaaata
     6241 atgactaact ttgaccaaaa ttacacgcca gttatgcata aaagagttcc gttattgtgt
     6301 ggattgagta tactcatttc tactattttt tcagcttgtt taacagagcc ttctacatca
     6361 caagaggcac atattcaatc tttttatttc aatgcaaatg attctgcacc ggatataagt
     6421 agtataactt tcacggtaga cacaacaaat cttattattt ttaatacaga ttctgttgcg
     6481 tatgattcta acataagtca gattgttccg gtgatagtag gatacggcag tttttcaggc
     6541 atcactgtaa attctattac atggaatcaa accgattctt tagattttag aaatcctgta
     6601 agtataaccg ttacggctgc cgacaaaaaa acatcccaca catacaatgt tgtagtaaac
     6661 aagcacaaag ttaacccgga tagcatcgta tggacaaaaa cagttgacgc tatcgctcaa
     6721 atagataata tcaaagcaac gcaggccttt tatgtcgaaa ataaattatt ctgtgctatt
     6781 caatcagaaa atcgtagcga catatacgta gcagatacgg aaactaattg gagttctgat
     6841 attaatagta caatagaatt taatgtcagg tcaataacag tgagagaaaa cgatatcgtt
     6901 atgcttacaa acgatggttc ggcaatgtat agttggaatc ctacaacaaa agcattagaa
     6961 caattggcaa atgcacctga aggatatgaa atcaaagatt taatcggata catagacgac
     7021 gaaatttggg ctatctctat aaacaatggc attaagacaa tcttaagcta tagtaacaac
     7081 gaatggaata acatatctga tgcatcaata gaagcagagg gatttcctgt tgaaggctct
     7141 gcaaaaatgc aatggaacaa cacaatgcca tacttgttag gaggttcaaa cgcatcagga
     7201 gtattgcaaa attgtgtgtt ttctaccgaa aatggtaaat actggaccaa tcttatcaaa
     7261 tcacctaatt atggatttgg caatcgcaaa gatgctgctg caatatatct agacaacaaa
     7321 atatttatat ttggaggaag caatggcagt acaattgtca gcactacata cctgtcacac
     7381 gataatggtt tcagttggtc gaatgcttta gaatatcaag aattacctac tacatacaca
     7441 gataaaacag gcataagtgc tgtagttgac aaccaaaatt acatttggct tattgggggt
     7501 aaagatgaaa acggatcttt tcacacagat gtatggaaag gtcgaatcaa taaatttgat
     7561 ttcttactgc aataatataa atggaacgca ttgctttatt tccaggcagt tttaatccgt
     7621 ttacaaaagg gcatgagtct atagttagca gatcactaag catatttgac aaaataatta
     7681 ttgctatagg tgtaaatgga gataaaaaca ttgaattcat cgataaaaga atggattcta
     7741 taagacaatt atatatagat aatccacgtg ttattgttgt aaattacgaa actctcacag
     7801 cagatgtagt aaaaaaatat aatgccaact gcatcattag aggtatacgt aatggtaaag
     7861 atttagagta cgaaacaaat atagcagaag tcaattactc attttttgga gtagaaacaa
     7921 tctttatgct cagtgaacca aatcttaaaa aggtttcatc aagtatagta cgagaattgc
     7981 aaaaatacgg taaagacgtt tcttcctacc taccaaataa ataataatta caaattattt
     8041 gcgttagaaa tcttattgac acgcgcagaa tggcgacctc cttcaaagtc agtagtcaaa
     8101 aaggactcaa tcataatttc agcctcagcc aatgaaataa aacgagcagg taatgatata
     8161 atatttgcat cattatgttt tcgagctaaa agagctatct cgtttttcca acataatgca
     8221 gcacgaacat tacgatattt attagccacc atactaacgc cattaccggt tccgcaaata
     8281 gctatgccaa aatcaaactt tcctgcatta atttcggcag ccatcggatt aatcatatca
     8341 ggataatcaa cactatcagc agagaatgtt ccaaaatctt tcaattcgaa caattttgct
     8401 ccaaaatact gtttaatctc ctctttcaat tcataaccgg cgtggtcaca agcaatagca
     8461 acacttaagg tgtctaaatc tttcatatac atcaaggttt aaaggtttac ctattcgggg
     8521 atatactatc ccttgtaaaa caagagcaaa gatagctcaa atcaatgaca aaaaagcaaa
     8581 agaattttat ttatttcaat aattattatg caattattga gataaaacta agaagccgtg
     8641 ttctttcaaa tcttcccaaa ataaaggata cgatttagat acaacattag ggttgttgat
     8701 taaaatattt tgtaagcaca tagctgcagg agcaaatgcc attgccatcc tatgatcatc
     8761 ataagtatct atactgaact cgcctgaagt ttctattttt aaaccgttcc aagacaattc
     8821 tccgattccg atttcctcta gcttaaatcc catttttgca cattcttttt tcaaagcagc
     8881 aattctgtct gtttctttta tttttaatga ttgcaatcca caaaaattaa attgtactcc
     8941 caaaaggcaa catgtaacta ctatggtttg agccaaatca ggacaagaat taaagtcgta
     9001 gaacagcttt tttacaggag ttgatgtttt ttttatccgc acattaccat taagatactc
     9061 agtttgtact cctaatgatt caaatattgt tgctaccaca gaatcccctt gtaaactatt
     9121 ctttgataga cctattagtt caagttcggc attttcagtt agcgaaatta tttcgtacca
     9181 atatgatgct gcagaccaat cattttctac tatataagat gtattcgaat atttctgtgg
     9241 aaatattttt atcacattat catgccatac tgtagaaacc ccaaaacttt ccatcataga
     9301 taaagtcatc ttaatgtagg gctttgaact aatttttccg ttcaaataca tattgatccc
     9361 attttgagta tatggggcaa tcataagtaa tgccgatata tactggctac taacactgcc
     9421 gtcaatttca atatcaccac caattaggct atgacccttt attcttaatg gaggaaatcc
     9481 ctcttcgtta agatattcaa tatctgctcc tatgttcctt aaggcatcaa ctaaaatctt
     9541 aatagggcga tgtaacattc gttctgatcc ggtaatttgc cattcgcctg ccttaagaga
     9601 caagtaagca gttaaaaatc gcattgcagt tccggcagca ccaacattta tctcgttttt
     9661 aaaagaagac aatgcagcaa caactgcatc tgtatcatca caacaagata gattttttac
     9721 atcagaactt ccggacaaag cttttaaaat taatgctctg tttgaaatac ttttagaagc
     9781 cggtaatatt acgcttccgg atatcttttt tgataatata atacaatttg atttcatttt
     9841 tgtctacaat gcccacaaaa atataaaaaa aggagcaaaa cggcatcgtt ttatttattt
     9901 ttaagacatg ttgtcaaatt atgaatttgc cattttagat attctgatta attttgcatc
     9961 gtgggtaaca agggagtgac cacataaaaa attattaaag agagaagttt ttaaacaata
    10021 aatcatttaa ctattttcaa acatggcaaa tttagattta agcaagtatg gcattaacgg
    10081 agccattgaa gtattacaca atccttcatt tgatgttttg tatgcagaag agacaaaacc
    10141 cggtttagaa ggttttgaaa aaggtcaagt aacagagtta ggagcagtaa acgttatgac
    10201 aggtgtttac acaggtcgtt ctcctaaaga taaattcttc gttaagaatg aagcaagcga
    10261 agatagccta tggtggacat ctgatgaata taaaaatgac aacaaaccat gttcagaagc
    10321 agcttgggcc gacttaaaag ctaaggcagt aaaacaactt tcaggcaaac gtctatttgt
    10381 aatagatact ttctgtggag ctaaccctgc aacacgatta aaagttcgtt ttataatgga
    10441 agtagcttgg caagcacact tcgtagcaaa tatgtttatt cgcccaacag atcaagaatt
    10501 agctaactac ggcgaacctg attttgtatc tttcaacgca gcaaaagcaa aagttgaaaa
    10561 ctttaaagaa ttaggattaa attcagaaac tgctaccgta tttaatttga aaacaaaaga
    10621 acaagttatt ttgaacacat ggtatggtgg cgaaatgaaa aaaggtattt tctctatcat
    10681 gaactacctt aacccattga agggtatagc ttctatgcac tgctctgcta acacagacaa
    10741 agcaggtact agtacagcta ttttctttgg tttatcagga acaggaaaaa caactttgtc
    10801 aacagatcca aaacgtttac ttattggtga tgacgagcat ggttgggaca acgaaggtgt
    10861 attcaactat gaaggtggat gctacgctaa agttatcaat cttgataaag atagcgaacc
    10921 tgatatttac aaagcaattc gtcgcgatgc tttgctagaa aatgttactg ttgatgcaaa
    10981 tggaaaaatt gatttcaatg ataagagcgt aacagaaaac acacgtgttt catatcctat
    11041 cgaacatatt gaaaacatcg ttaaacctgt atcgaaaggt cctcacgcaa aacaagttat
    11101 tttcttgtca gctgatgctt tcggagtatt acctcctgta tcgattttga cacctgaaca
    11161 aacaaaatat tatttcttat caggatttac agcaaaatta gcaggtacag aacgtggaat
    11221 tacagaacct accccaacat tctctgcttg ctttggtgca gcattcttaa gtttacatcc
    11281 tacaaaatat ggtgaggagt tggttaagaa aatggaaatg acaggtgcaa aagcatactt
    11341 agttaacaca ggttggaatg gaacaggaaa acgtatttct attaaagata ctcgtggtat
    11401 aatcgatgct atcttagacg gaagtataga caaagctcct acaaaacaaa tccctatgtt
    11461 tgacttcaaa gtgccaacag cattacctaa cgtagatcct aagattttgg atcctcgcga
    11521 cacttatgca agcgcatctc aatgggacga aaaagctaaa gatttagcag cacgtttcat
    11581 caagaacttt gctaaattcg aaggtaacga agccggaaaa gcattagttg ctgccggtcc
    11641 taaattgtaa ttgattaatc tttttattaa taatagcccc gaagtttaag actttcgggg
    11701 ctatttattt atttaatatg gatgtaagtt ttacatccat attttacaaa aaaatactaa
    11761 ctttgcagtt agcataacat catactttta aaaacaataa tttgatatat gattgaagat
    11821 acctcattga tattacaaga tctaaaattc aaaaacacat atacagacga ttcgattaaa
    11881 actctggagg gacttgaaca tatccgctta agacctggta tgtacattgg aaaattgggt
    11941 aatggtgaat atgctgatga tggtatttat gttcttttaa aagaagttgt agat
//
LOCUS       DAOPID010000019        11971 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_6364_length_11971_cov_3.759987, whole genome shotgun sequence.
ACCESSION   DAOPID010000019 DAOPID010000000
VERSION     DAOPID010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 11971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11971
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6364_length_11971_cov_3.759987"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2436
                     /locus_tag="PK641_01480"
     CDS             <1..2436
                     /locus_tag="PK641_01480"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HPG55060.1"
                     /translation="NNDNSCSQISNEIIITDSSDRPSATITTTRTELTCAISSITLTA
                     ETDAANVMYYWSTTESTSSIEVSGAGDYTVTITNLDNHCSSTSLPVEITENKVVPLAN
                     VTSSATELTCTTTSITLTASTDVTNASYLWSTNETTSEIDVTTAGNYSVEVTNEDNGC
                     STISLPVSITEDKVAPTATISSTETVLTCIRTSITLTAVTNATNASYLWEDGSAQISF
                     DATSTGNYSVEITNNDNGCSTTSNSIVITEDVNKPVAAIQSLSTIITCDQTSIDLQAL
                     TDAVNASYLWSEGSVDPAINVNAAGNYTVTVTNTDNGCSSIGLPIEITENTKIPDVQI
                     NSSNSELNCIVHTITLSAITDAVNPSYLWNDGSYNEDLLVSAAGIYSVSVTDQVNGCS
                     FTSGPVNITENTVLPTAAITSLETELTCLITSITLTAVTDAVNPEYEWSNGSNLASLS
                     VDAAGNYTVTVTNLNNGCETTSLPITITSDIIVPQISLQVIDPVCSPETVNLSDAVLS
                     SDADNIHYYEDAALQQEVTDLLISIPGEYRYYIVGENNNGCKSIPQSVSIGIYELQVS
                     NFVSANICAGETVVLWAEGTGPQADMYVYNIHEPGDVLSGNKTDNRFEVNVTPYQTTT
                     YSLSVVNGVCQYDADMNVLVWPLPSFRVEQTDPQTATLNMLDVSESPYVYMLDGTEMF
                     FDTDPPYVFTVNAGDHEMYVSDINGCENVIEFFVDNSKMPIMIPKFFSPNGDGQNDTW
                     EIGNIDYYPNALIQIYDRYHKLIIEYKGSDRGWDGTYKGHQMPSTDYWYLIKIPELGK
                     PQVGHFSLLRGKL"
     gene            2600..4006
                     /locus_tag="PK641_01485"
     CDS             2600..4006
                     /locus_tag="PK641_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55061.1"
                     /translation="MIVFVCPYANKLFAWNGDTDKFKIDNLELSLNAPGESGIAYISE
                     ESSYNYNATWECTLSFDFTPSSANYVKWYLISDQEDLTSSLNGYFLRIGYTDKNICLY
                     RQNGQSNIKLAEGQAGRLSTPKSKIELRILRDVKGLWTVRSRLSDESNFIDEFETIDN
                     THERCKYHGFVCVYTSTRSKGFHFTSLYASGNEYIDILPPKPLEIVFNEILFRPFDDG
                     SDFVELYNNSGNILQLSSVKIANRKKEIMLPSYKLYPNAYVVLCSNKDAVCAFYQCKS
                     DTLFVETDLPSMPNDSGYLALLSEDGLVLDSLLYESSMHQALVINNKGVSLERINQNS
                     SSWTSASYSVGYATPGYKNSQFTDFDYANSDTIEWTSSELFSPDNDGNNDVLYVYYKT
                     KVGTYANVWVYHSSGYPIKQLCKNELLSSSGTILWDGRNESGKLCSIGIYVICIETYS
                     EDGISAKKKIPIVLSAKK"
     gene            4067..5581
                     /gene="gpmI"
                     /locus_tag="PK641_01490"
     CDS             4067..5581
                     /gene="gpmI"
                     /locus_tag="PK641_01490"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HPG55062.1"
                     /translation="MAKKALLIILDGWGIGNRDKADIIYNTPTPYWDSLLKNYPNSQL
                     QASGENVGLPDGQMGNSEVGHLNIGAGRVVYQDLVKINLACRDNSILKNEEIVSAFSY
                     AKNTGKSVHLMGLTSDGGVHSSLDHLFKLCDIANSYNIENTFIHCFMDGRDTDPKSGK
                     GFIEQLDAYTKNITAKIASIVGRYYAMDRDKRWERVREAYDLLVNGTGKAAGCMVKAM
                     QESYDEGVTDEFVKPIVNKSVDGRIKAGDVVIFFNYRNDRAKELTVVLTQQDMPEAGM
                     KTIPDLQYYCMTPYDSSFKGVHILFDKDNVSNTLGEYLSNQGKKQLHIAETEKYAHVT
                     FFFNGGREAPYKNEERILINSPKVATYDLKPEMSAYEVKDALVTEIDKQKFDFIVVNY
                     ANGDMVGHTGIYDAITKAVVTVDACVRDTVEAAKRNDYEVIIIADHGNADNAVNPDGT
                     PNTAHSLNPVPFVYVTSNKSAKVQNGILADVAPSILNIMGMAQPAEMTGKNLID"
     gene            5715..5876
                     /gene="rpmH"
                     /locus_tag="PK641_01495"
     CDS             5715..5876
                     /gene="rpmH"
                     /locus_tag="PK641_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HPG55063.1"
                     /translation="MKRTFQPSNRKRANKHGFRERMSTPNGRRVLAARRAKGRKKLTV
                     SDEAAIRRN"
     gene            6027..6659
                     /locus_tag="PK641_01500"
     CDS             6027..6659
                     /locus_tag="PK641_01500"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HPG55064.1"
                     /translation="MKKLIDFFKRHNFLRNLLYISIASFLLIVGVLWWLNIYTHHGES
                     EIVPNIKNLKMESAVKLLEGKSLNYEVVDSVYKKGFPPGNVVEQDPQPGSFVKKDRKI
                     YIVINARSVQMLPLPSASDISLRQSKSILEGSGFKVTEIEYKPSAYKDLVLSVQYKGR
                     EVFAGDRIPAESALVLWVGSGSLEEIVEELDTLSSEDIIGESSLEEEIVF"
     gene            6659..7711
                     /locus_tag="PK641_01505"
     CDS             6659..7711
                     /locus_tag="PK641_01505"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPG55065.1"
                     /translation="MLNEEYIDEIQEDDDVNSRYEHFRFTADPGQSLVRIDKFLTDRI
                     LNVSRTRMQAAADAGNLEVNGKVVKANYRVKPNDVVVVMLDYPPRDTTIIPQDIPIDI
                     VYEDDDLLVVNKAAGMVVHPGHGNFDGTLLNALAYHLGEKNAFDASDPRLGLVHRIDK
                     DTSGLLLIAKNPYAKMFLSQQFFHKTTDRLYQALVWGTTDAEGTIEGNIGRSPKDRLQ
                     MAVFPEGNVGKPAVTHYKLLENFHYVSLVECKLETGRTHQIRAHMKYIGHPLFNDVRY
                     GGDQILKGTTFAKYKQFIQNCFDICPRQALHAKTLGFEHPTTHERMYFNSELPEDMKN
                     LLDKWRNYTTCIIFAD"
     gene            7739..8374
                     /gene="pyrE"
                     /locus_tag="PK641_01510"
     CDS             7739..8374
                     /gene="pyrE"
                     /locus_tag="PK641_01510"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HPG55066.1"
                     /translation="MEQVEAMVAARLLAIKAVKLQPNQPFTWASGWKSPIYCDNRKTL
                     SYPDLRSLIKLELSRIILEKYPDVEAIAGVATGAIAQGALVADALGLPFVYIRSAKKD
                     HGMENLVEGYLPEGKKVVVVEDLISTGGSSLKAVEAIRSEKKCEVLGMVAIFTYGFPL
                     AQEQFEKAGVELTTLSNYEAVLKHALATNYINNKDLAALQEWRKDPSVWQV"
     gene            8377..8787
                     /locus_tag="PK641_01515"
     CDS             8377..8787
                     /locus_tag="PK641_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HPG55067.1"
                     /translation="MTTYESQIKSINASAEAIFNNLSDLEKLRPVVEQVHDDRIKDIN
                     ITSDSIYCTIDPIGEIGVKMVETEAFKTIKFASEKSPIEFNMWIQLVEKSENDTRMKL
                     TIKADVPLMFKMMLSEPLSKGIDILAENLSKLPY"
     gene            8798..10126
                     /gene="argH"
                     /locus_tag="PK641_01520"
     CDS             8798..10126
                     /gene="argH"
                     /locus_tag="PK641_01520"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HPG55068.1"
                     /translation="MASKLWDKKIQLDSDIERYTIGKDAEMDMYLARYDVLGSMAHAT
                     MLESVGLLSNDDLQALLKGLRSIYKEIEKGNFKIEDGVEDIHSQVELMLGEVGKKLHS
                     GRSRNDQVLVDLKLFTRAKIQELVASVNDLFETLIAQSNLYKDVLMPGYTHLQIAMPS
                     SFGLWFAAYAESLVDDMQLLLAAYKISNRNPLGSAAGYGNSFPLDRQLTTDLLGFDSM
                     DYNVIYAQMGRGKTERIVANALASVAATLSKLAFDSCMFTSQNFGFMKLAPEYTTGSS
                     IMPHKKNPDVFELTRAKCNKIQGIPAQITLITNNLPSGYFRDLQIIKEIYLPMFDEMI
                     DCVEMVRNMMEKVSVNKSILEDSKYDLIFSVEEVNRLTLSGVPFRDAYKQVALAIEDG
                     KFKPNKEIQHTHAGSIGNLCNDKIKSLMSSLLTEFPFERVENAEKQLIKD"
     gene            10129..10890
                     /locus_tag="PK641_01525"
     CDS             10129..10890
                     /locus_tag="PK641_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPG55069.1"
                     /translation="MLFHSTIFGPIYSRRLGVSLGVNLEPDNGKICSFDCVYCECGLN
                     ADGREDTKIPTRAEVKKALENKLVTYIKGNEPTIDNITFAGNGEPTMHPDFNGIIDDT
                     IELRNRYTPETKISVLSNAWHLSRKEVFTALCKVDNNILKLDSAIAETVSKINRPVNP
                     NFNLQEHINNLSKFNGNCTIQTLFLRGNTCDNTTPEEIKAWLDALKIIQPKMVQIYSL
                     DRKTPYDTLQAVPLSELKSIEVKVRKLGLNTLVVA"
     gene            10887..11843
                     /locus_tag="PK641_01530"
     CDS             10887..11843
                     /locus_tag="PK641_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55070.1"
                     /translation="MIPKKRILVCPLDWGLGHATRCIPLINSFLSQGDEVIIGSCGLS
                     GNLLKDEFPDLQHISIPSFSIRYSAGNSQVFSMLRALPSIIYFSFREHSLLKSIVKQY
                     KITDVVSDNRFGMWCKDAKCTYITHQLLVRMPQKFRWLEPIVSRLHRSIINNYDSCWI
                     PDNENIRESLSGELSHSCRLPKNAKFIGPLSRISKEGASIESDVLIILSGIEPHRSIL
                     EKKLLERYSKHQSKVILVEGIPMQNNTVRELPNITIYPSLNSDVLSEYIRGAKHIICR
                     SGYTSIMDMHAAGKFDVVEWIPTPGQPEQEYLSLWLNVKKTL"
BASE COUNT         4068 a         1806 c         2381 g         3716 t
ORIGIN      
        1 aataacgata attcttgttc acaaataagt aacgaaatta tcattacaga tagttctgat
       61 cgaccaagtg caacaataac aactacgagg acagagctaa catgtgcaat aagctcgata
      121 acattgacag cagaaacaga tgctgcaaat gtaatgtatt actggagtac tacagaaagc
      181 acctcaagca tagaagtatc aggagcagga gattacactg taaccataac taatttagac
      241 aaccattgtt cgtcaactag tttgccggtt gaaattactg aaaataaagt agttccattg
      301 gctaatgtaa catcttcagc cacagaacta acttgtacaa caacttcgat aacattaaca
      361 gcatcaacag atgtgactaa tgccagttat ttgtggagta caaacgagac gacttctgaa
      421 atagacgtga ctactgccgg taattattcg gtggaggtaa caaatgagga taatggttgc
      481 tcgacaataa gtcttcctgt ttctattact gaagataaag ttgctcctac tgcaacaatt
      541 tcatctaccg aaacagtact tacatgtata agaacatcaa taacattgac agcagtaaca
      601 aatgcaacta atgcaagtta tttatgggaa gatggaagcg cgcaaataag ttttgacgct
      661 acttcaacag gtaactattc tgttgagata acaaataacg ataatggttg ttcaactacg
      721 agcaattcaa tagtaattac agaagatgtt aacaagccgg tggctgccat acaatcatta
      781 agtactataa ttacatgtga tcagacatca atcgatttac aagcattaac agatgctgta
      841 aatgctagtt atttgtggag tgaaggtagt gttgatcctg caataaatgt aaatgcagca
      901 ggtaattata cggtaacagt aactaatact gacaatggct gtagttcaat aggtttacca
      961 attgaaatta ctgaaaatac taagatccct gatgttcaaa taaattcttc aaacagtgaa
     1021 cttaattgca ttgttcatac aataactctt agtgcaataa ctgatgcagt aaatcctagt
     1081 tatttgtgga atgatggaag ttataatgag gatttgttag tttcggcggc gggaatatat
     1141 tctgttagtg taacagatca agttaatggc tgtagtttta caagtgggcc tgtaaatatt
     1201 actgaaaata cagttttacc tacagccgca attacatcgc ttgaaacaga acttacatgt
     1261 ttgataacca gtataacatt aacagctgtt acagatgccg tgaatcctga atatgaatgg
     1321 agtaatggat ctaacttggc aagtctttcg gttgatgcag caggaaacta tacagtaact
     1381 gtaaccaatt taaacaacgg atgcgaaaca actagtttgc ctataacaat tactagtgat
     1441 ataattgtac ctcagattag tttacaagta atcgatcctg tatgttctcc ggaaacagta
     1501 aatcttagcg atgcagtttt gtcaagtgat gctgataaca tacattacta cgaagatgca
     1561 gccttgcaac aggaagtaac agatttatta ataagtattc cgggagaata cagatattat
     1621 attgttggtg aaaacaataa tggatgtaaa tctattccac aatctgtttc tattggaatt
     1681 tatgaattgc aggttagtaa ctttgtttcc gctaacattt gtgccggaga aactgttgtt
     1741 ctatgggcag aaggtacagg accgcaagca gatatgtatg tttataatat tcatgaaccg
     1801 ggtgatgtac taagcggcaa taagacagat aatcgtttcg aagtaaacgt aacgccatat
     1861 caaactacta cttattcatt gtctgtagta aatggagtat gtcaatatga tgctgatatg
     1921 aatgttttag tatggccatt accatctttc agggttgaac aaacagatcc gcaaacagct
     1981 actttaaata tgctggatgt ttcagaatca ccgtatgtat atatgttaga tggtactgaa
     2041 atgttttttg atacggatcc accatatgta tttactgtaa atgcaggaga tcatgaaatg
     2101 tatgtttcgg atataaatgg atgtgagaat gtaatagaat tctttgttga caactctaag
     2161 atgcctataa tgataccaaa attcttcagc ccaaatggtg atggccaaaa tgatacatgg
     2221 gaaatcggaa atattgatta ttatccgaat gctttgatac aaatatatga tcgttatcat
     2281 aaattaataa ttgaatacaa aggctctgat agaggatggg atggaacata taaaggtcat
     2341 caaatgccat ctacagatta ttggtattta atcaagatac cggaattagg taaaccacaa
     2401 gtcggacatt ttagcttgtt gagaggaaaa ttataattta aaaatatggc aaactttttt
     2461 agcttgccat atttttttat cccaacccaa ttgtgagttc gagcaaaaaa ttagtaaatt
     2521 tgccatgcat ttgctaatgc gattattcac atctaattct tacatctata tgttacattt
     2581 taagtacact gcgttttgtt tgatcgtttt tgtatgtccg tatgcaaata aattatttgc
     2641 gtggaatggt gatacagata aattcaaaat agataattta gaactgagtt taaacgctcc
     2701 gggcgaatca ggtattgcat atatttcgga agaatcttca tacaactaca acgcgacgtg
     2761 ggagtgtact ttgtcttttg attttactcc cagttcggca aattatgtta agtggtattt
     2821 gatttcagat caagaagatt tgacatcatc attgaatggc tattttttaa gaatcggata
     2881 taccgataag aatatatgtc tatatcgaca gaatggtcaa tcaaatataa agcttgcaga
     2941 aggtcaggcc ggacgattat ctactccaaa atcaaaaatt gaattaagaa tactgcgtga
     3001 tgtaaaagga ttatggactg ttcggtctcg tttatcagat gaatcaaatt ttatagatga
     3061 gttcgagact attgataaca cccatgaaag atgcaaatat catggatttg tttgcgttta
     3121 tacgtctact cgcagtaaag gatttcattt cacttcatta tatgcatccg gaaatgaata
     3181 cattgatatc cttcctccta aaccattaga aatagtattt aatgaaatac tttttagacc
     3241 atttgatgat gggagcgatt ttgttgaatt gtataataat tcaggtaata tattacaatt
     3301 aagcagtgtg aaaattgcga atagaaaaaa agaaattatg cttccttcat ataaattata
     3361 ccccaatgca tatgtggtat tgtgttcgaa taaagatgcc gtatgtgctt tttaccagtg
     3421 taagtcagat acgctgttcg tagaaacaga tttgccaagc atgccgaatg attcaggtta
     3481 tttggcatta ttatcggaag atggattggt cttagatagc ttgttatatg aaagttctat
     3541 gcatcaagct ctagtaatta ataacaaagg cgtatcgctc gaacgtatta atcaaaatag
     3601 ttcatcttgg acttccgctt catatagtgt aggatatgct actcccggct ataaaaactc
     3661 acaatttaca gactttgatt atgctaattc tgatacaatc gaatggactt cttcagaatt
     3721 attcagtcct gataatgatg gcaataatga tgttctgtat gtttattaca aaacgaaagt
     3781 cggaacttat gcaaatgtgt gggtatatca ttcctcaggt tatccgataa agcaattgtg
     3841 taaaaatgaa ttgctttcat caagcggaac aattttatgg gatggtagaa atgaatcagg
     3901 aaagctttgc tcaataggaa tatatgtgat atgtatagag acatattcgg aagacggaat
     3961 tagtgcaaaa aagaaaattc ctattgtgtt gtctgctaaa aaataagcta attcatcttt
     4021 tttacgtaca tttgtgcttc gaaaataata taaacaatat acaacaatgg caaagaaagc
     4081 actattaatt atcctagacg gatggggaat cggaaatcgt gataaagcag atattattta
     4141 caatactccg acaccatatt gggactcatt gttaaagaat tatccaaatt cacaattgca
     4201 agcatcaggt gaaaatgtag gtttacccga tggtcaaatg ggaaattctg aagttgggca
     4261 ccttaacatt ggtgcaggta gagtagttta tcaagatttg gtgaaaatca atttagcatg
     4321 tcgtgataat tctattttga aaaacgaaga gattgtatca gcttttagct atgcaaagaa
     4381 taccggtaaa tcagttcatc ttatgggtct aacttcagat ggcggagtgc atagttcttt
     4441 agatcatttg tttaaattat gtgatatcgc taatagctat aatattgaaa atacatttat
     4501 tcactgcttt atggatggtc gtgatacaga tcctaagagt ggaaaaggtt ttattgaaca
     4561 attagatgct tatactaaga acattacagc taagatagca tctattgtag gacgttatta
     4621 cgcaatggat cgtgataaac gttgggaacg tgtacgtgag gcatacgatt tattggtaaa
     4681 cggaaccggt aaggctgctg gttgtatggt gaaagctatg caagagtctt atgatgaagg
     4741 tgttacagat gagttcgtaa agccgattgt aaacaaatcg gttgatggac gtattaaagc
     4801 cggtgatgtt gtaatcttct tcaattatag aaacgataga gcaaaagagt tgacagtagt
     4861 tttaactcaa caagatatgc cagaggcagg gatgaaaaca attcctgatt tgcagtatta
     4921 ttgcatgact ccttatgatt catcatttaa aggagtacat atattgtttg ataaggacaa
     4981 tgtttcgaat accctaggag aatatttatc aaatcaaggc aagaaacaat tacatatagc
     5041 tgaaacagaa aagtatgccc atgttacatt tttctttaat ggtggtagag aggctccata
     5101 taaaaatgag gaacgtatct tgattaactc accaaaggtt gcaacatacg acttaaaacc
     5161 cgaaatgagt gcatatgaag ttaaagacgc tttggttacc gaaatagata agcagaaatt
     5221 cgatttcata gttgttaact atgcaaatgg ggatatggtt ggccataccg gaatatatga
     5281 tgcaataact aaagcagttg taactgttga tgcttgtgtt cgcgacacag ttgaagctgc
     5341 taaacgtaat gattatgagg ttattataat agcagaccac ggaaacgcag acaatgcagt
     5401 taatcctgac ggaactccaa atacggcaca ctccctaaat ccggtacctt ttgtatatgt
     5461 aacgtcaaac aaatcagcta aggtacaaaa cggaattttg gcagacgttg caccatcgat
     5521 tttaaatatt atggggatgg ctcaaccggc cgaaatgacg ggtaaaaatt taattgatta
     5581 attaagcgac tcttttttga gcagattgtt gtgtgttttt ataaaaaaaa gctcaataat
     5641 tttgttgatt ttggaaatag tgttaccttt gccgtcccga acattggaaa gaaataaata
     5701 aaatatagat aattatgaaa cgtacatttc aaccttcaaa cagaaaaaga gccaacaaac
     5761 acggttttcg tgaaagaatg tctactccta acggtcgccg cgtattagct gcacgccgtg
     5821 caaaaggcag aaaaaaactt actgtatctg acgaggctgc aataagacgt aactaattat
     5881 attgaagata atatcttcga tatacaaccc ataaactaac ggggcgaaat tataatttcg
     5941 ccccgtttct tgtacttatc tgtgatatta taaacgaaat tacattatct ttgcctatct
     6001 gttagtgata atccaaaacg aactacatga aaaaactgat tgattttttt aagagacata
     6061 attttctaag aaatttgttg tacatatcaa tagcatcttt ccttcttatt gttggtgttc
     6121 tatggtggct aaatatatac acacatcatg gtgagagtga aatcgtgcca aatatcaaga
     6181 accttaaaat ggaatcagca gtaaaattat tagaaggcaa aagtctgaat tatgaagttg
     6241 tggattctgt atataaaaaa ggtttccctc ccggaaatgt tgttgagcaa gaccctcagc
     6301 cgggttcttt tgtaaaaaaa gatcgaaaaa tatatatagt aataaatgca agatctgtgc
     6361 agatgttacc attgccttcg gcatcagata tatcattacg tcagtcaaaa tcaatattgg
     6421 aggggtcagg atttaaagta acagaaattg aatataagcc atcagcatat aaagatttgg
     6481 tgttgagtgt tcaatataaa ggtagagaag tttttgccgg tgaccgtatt cctgccgagt
     6541 cggcattggt tttatgggta ggaagtggct ccttagaaga aatagtggaa gagttagata
     6601 ccttatcttc agaagatata attggtgaaa gtagtcttga agaagaaata gttttctaat
     6661 gctaaacgaa gaatatatag acgaaataca agaagatgat gatgtaaata gtcgttatga
     6721 gcattttcgt tttactgccg atccgggtca aagtttagtt agaatagata agtttttgac
     6781 agatagaata cttaatgtgt caaggactag aatgcaagca gctgctgatg ccggaaactt
     6841 ggaagttaac gggaaggtag taaaggctaa ttatagagtt aaacccaatg atgtggttgt
     6901 agttatgctg gattatccac cgcgtgatac aacaattatt ccacaagata ttccaattga
     6961 tattgtatac gaggatgacg atttgttagt cgtgaacaaa gcagccggaa tggttgtgca
     7021 tcccggacac ggtaattttg acggaacact acttaatgca ttagcatatc atcttggtga
     7081 aaagaatgct tttgatgcct ctgaccctcg tttaggtttg gtgcatcgta tcgataaaga
     7141 cacttcaggt ttattattga tcgcaaaaaa tccttatgca aaaatgttct tgtctcaaca
     7201 atttttccat aaaactacag atcgtttata tcaagctttg gtgtggggga caaccgacgc
     7261 agaaggaacc attgaaggta atataggaag aagtccgaaa gaccgtttgc aaatggcagt
     7321 tttccctgaa ggtaatgtcg ggaaaccggc tgtaactcat tataagttat tggagaattt
     7381 tcattatgtg tcgttggtag aatgcaaatt agaaacaggc agaactcatc agataagagc
     7441 acacatgaaa tatatcgggc atcctttgtt taatgatgtt aggtatgggg gagatcagat
     7501 tctaaaaggt acaacatttg ccaaatataa acagtttata caaaattgtt tcgatatatg
     7561 tccgcgacaa gcattacatg caaaaacatt aggctttgag catcctacaa ctcatgagcg
     7621 tatgtacttt aattctgagt tgcccgagga tatgaaaaac ttgttggata aatggagaaa
     7681 ctacacaact tgtattatct ttgcagacta aacttattgc agaatatata tatttaatat
     7741 ggaacaagta gaagctatgg tagcggctcg attgttagca attaaagcag ttaaattgca
     7801 gccgaatcag ccatttacat gggcatcagg gtggaaatca ccgatttatt gtgataatcg
     7861 taaaacattg tcatatcctg atttgcgcag tttaatcaaa ttggaattgt cgcgtattat
     7921 cttggaaaaa tatcctgatg tagaggcaat agccggtgta gcaacaggtg caattgcaca
     7981 aggcgcatta gtagccgatg ctttaggttt accatttgta tatattagat ctgcaaagaa
     8041 agatcatggt atggaaaatc tagttgaagg ctatctacct gaaggcaaaa aggtcgtagt
     8101 tgtagaagat ttaatatcta ccggaggtag tagcttaaaa gctgttgagg ctatcaggtc
     8161 tgagaaaaaa tgtgaagttt tagggatggt tgcaatcttt acttatggtt ttccattagc
     8221 acaagagcaa tttgagaaag ccggagttga actgactaca ttgtcaaatt acgaggctgt
     8281 tcttaaacat gcattggcaa ctaattatat taataataaa gacttggctg ctttgcaaga
     8341 atggcggaaa gatccaagtg tttggcaggt ataaaaatga caacatacga gagtcaaatt
     8401 aaatcaataa atgcatcagc agaggcaatt tttaataatc tgtctgattt agaaaaattg
     8461 cgacctgttg ttgagcaagt gcatgatgat aggattaaag atattaatat aacatcagat
     8521 agtatatatt gcacaattga tcctataggc gagataggtg ttaaaatggt agaaacagaa
     8581 gcatttaaaa ctattaaatt tgcatcagag aaatcgccta ttgaatttaa tatgtggata
     8641 caattggtcg agaaatcaga gaatgatact cgtatgaagc taactataaa ggctgatgtg
     8701 ccattaatgt ttaaaatgat gcttagcgaa cctttgtcaa aaggaattga tatattggca
     8761 gaaaatttat ctaaattacc atactaaatt tatcaaaatg gcaagtaaat tgtgggataa
     8821 gaaaatccag cttgattcgg atattgaacg ttacacaata ggcaaagatg ccgaaatgga
     8881 catgtatctt gctcgttatg atgtattggg ctcgatggct catgcaacta tgcttgaaag
     8941 tgttgggttg ctgtctaatg atgatttaca agcactgtta aaaggtttac gttcgatata
     9001 taaagaaatc gaaaaaggta attttaaaat tgaagatgga gttgaggata tacattcgca
     9061 agttgagttg atgcttggcg aagtaggtaa gaaattacat agtggacgtt ctagaaatga
     9121 tcaggtactt gttgatttga aattatttac tcgtgcaaaa attcaagaat tagtagcttc
     9181 tgtaaatgat ttatttgaga ctcttattgc tcaaagtaat ttatataagg atgtgttgat
     9241 gccgggttat acacatttgc aaatagcaat gccatcgtct ttcggattat ggtttgctgc
     9301 atatgcagag agtttggtag acgacatgca attgttgctt gctgcatata aaatttctaa
     9361 cagaaatcct cttggttctg ctgcaggata cggaaattca ttccccttag acagacaatt
     9421 gactaccgat ttattaggct ttgacagcat ggattataat gttatttatg ctcaaatggg
     9481 taggggaaaa acagaacgta ttgttgcaaa tgcattggca tcagtagcag ctactttatc
     9541 aaaattggca tttgatagct gtatgtttac aagtcaaaac tttggattta tgaagttggc
     9601 tcctgaatat accacaggtt caagtattat gccgcataag aaaaatcctg atgtatttga
     9661 acttacacgt gcaaaatgta ataagattca aggtattcct gcacaaataa cactgataac
     9721 taataattta ccatccggat atttccgcga tttgcaaata atcaaagaaa tttacttacc
     9781 tatgttcgat gaaatgatag attgtgttga gatggtaaga aatatgatgg agaaagtaag
     9841 tgtgaataaa tctattttgg aggattcaaa atacgattta atatttagcg ttgaagaagt
     9901 taatcgtcta actttatccg gggtaccttt ccgtgatgca tataaacagg tcgctttagc
     9961 aatcgaagat ggtaagttta aaccgaataa agaaattcaa catacacatg caggtagtat
    10021 aggaaattta tgtaacgata aaattaaatc tttgatgtca tctttattga cagaatttcc
    10081 tttcgaacgt gtagaaaatg cagaaaaaca attaattaag gattaatcat gttatttcat
    10141 tcaacgattt ttggaccaat ttacagtcgc agactggggg tctcgctggg agtgaattta
    10201 gagcctgaca atggtaaaat ttgttcgttt gattgtgttt attgcgaatg tggtttaaat
    10261 gcggatggtc gtgaggatac aaaaattccg acacgtgccg aagtcaaaaa agctttagaa
    10321 aacaaacttg ttacttacat taaaggtaat gagccgacaa ttgataacat aaccttcgca
    10381 ggtaatggag agccgactat gcatcctgat tttaatggta ttatagatga tactattgaa
    10441 ttaagaaatc gttatactcc tgaaaccaaa atatctgttc taagcaatgc ttggcacttg
    10501 tctcgcaagg aagtatttac tgctttatgc aaagtagaca ataatatctt gaaattagat
    10561 agcgcaattg ctgaaactgt ttcaaagatc aacagacctg ttaatcctaa ctttaacttg
    10621 caagaacata taaataattt gtctaagttt aatggaaatt gtactattca aactttattc
    10681 ttgagaggaa atacatgtga taatactact cctgaagaaa ttaaggcgtg gttggatgct
    10741 ttaaagatta ttcagcctaa aatggttcaa atatatagtt tagacaggaa aactccgtat
    10801 gatacattac aagcagttcc tttgtcagaa ttgaaatcaa tagaggtaaa ggtacgcaaa
    10861 ttaggcttga acactttggt ggttgcatga tacccaagaa acggatattg gtttgtccat
    10921 tagattgggg attggggcat gcaacgagat gtattccatt aatcaacagc tttttatcgc
    10981 aaggtgatga ggttattatc ggctcctgcg gtttatcggg taatttactt aaggatgaat
    11041 ttcctgattt gcaacatata agtatacctt ctttttcaat tcgttattct gcaggtaatt
    11101 ctcaagtctt ttctatgcta cgtgcattgc cttcgataat ttatttctca tttcgtgagc
    11161 atagtcttct aaaatctatt gtaaaacaat acaaaataac tgatgttgtt tctgataatc
    11221 gttttgggat gtggtgtaaa gatgcaaaat gcacttacat aactcatcaa ttattagttc
    11281 gcatgccaca aaaatttaga tggttggaac ctattgtatc tcgtttacat cgctcaataa
    11341 taaacaatta tgatagttgt tggattcctg ataatgaaaa tataagagaa tcattgtccg
    11401 gcgaattaag tcatagttgt agattgccta aaaatgcaaa gtttattgga cctttgtcgc
    11461 gtatatcgaa agaaggggca tctatcgaat ctgatgtttt aataatacta tcaggaattg
    11521 aaccgcatcg ttcaatatta gagaagaaat tattagaacg ttattcaaag catcaatcta
    11581 aggtaatact agttgaaggc atccctatgc aaaataacac tgtaagagaa ttgcctaata
    11641 ttacaatata tccatcgctt aattctgatg tcttgtcaga gtacatacgt ggcgcaaaac
    11701 atattatttg tcgttccgga tatacttcaa taatggatat gcatgctgca gggaaatttg
    11761 atgttgttga atggattcca actccgggac agcctgagca ggaatattta tccttgtggt
    11821 taaatgtcaa aaagactctc taacacaaaa atagtgttta gatttcccat agttggaata
    11881 tggtgtttgt aatatttgag taagctgtcc attagttgtc gcctttctgc atgagaaaat
    11941 tgttcttctt tgtttaataa gcgaatcaat a
//
LOCUS       DAOPID010000020        11839 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_6470_length_11839_cov_4.731415, whole genome shotgun sequence.
ACCESSION   DAOPID010000020 DAOPID010000000
VERSION     DAOPID010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 11839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11839
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6470_length_11839_cov_4.731415"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="PK641_01535"
     CDS             complement(<1..721)
                     /locus_tag="PK641_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HPG55071.1"
                     /translation="MNALRNLIDKIKPTFSEGGKLSMFRSVFDGFETFLFVPNETSKP
                     IGAQIHDAVDSKRIMIMVVIALIPALLFGMYNIGYQHFLSIGQLATTGFFPMFWYGFL
                     SVLPKIIVAYAVGLGIEFTVAQWKGEEIHEGFLVTGFLIPMIVPVDCPLWIMAIATAF
                     AVIFAKEIFGGTGMNILNVALITRVFLFFAYPTKMSGDSVWIRTDDVCGIGEGQLVDG
                     FTGATPLGQLATATGSTINLTD"
     gene            complement(746..2095)
                     /locus_tag="PK641_01540"
     CDS             complement(746..2095)
                     /locus_tag="PK641_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005836451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HPG55072.1"
                     /translation="MAGVIKLKKGLDIRLDGASELVYGRASQSEMIGFNPDDFYGMVP
                     KPVLKPGDAVKIGTVLFVDKNHPNLKVVSPVSGELVAVNRGERRKILDIVVKNDFKMK
                     QESIKAVDLNSSADEIKQSLSDAGMFAFIKQRPYDVMANPDDTPKAIFISTFDNAPLS
                     PDFDFIFKNEQSDFQNGIDALSKIAPVYVGIKNGTTTFSHVKNATVTTFEGNYPSCNV
                     GVQINKVNPINKGDIVWTITTQDVVCIGRFVGKGLLDFSRTIVLAGTEVDQKAYYKMI
                     LGESLAQILKANVYESTARIISGNPLSGKQTTVDAWLSPFVSQITALKDGKDEHEFIG
                     WIMPRFDKYSESGSFFTKFLMKLCPKKTFNFDTRILGGHRQMIMSGEYDKVMPMDIYP
                     EYLIKAILAGDIDKMEALGIYEVIPEDFAAAEFVCSSKMELQRIVREGLDMLRKEMC"
     gene            2377..3651
                     /locus_tag="PK641_01545"
     CDS             2377..3651
                     /locus_tag="PK641_01545"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HPG55073.1"
                     /translation="MKKFSLIYIACMILTYSFADNIVYETKSLNKNIKTVQIENFSRK
                     PYYPIIELGGKDKVTLSFDDLSPEINTYSYSIIHCNADWTKSKLNEFQYLNGFDGGFI
                     RNYESSVNTYFPYTHYTLTFPNEDVTCTVSGNYIISVYPDNDKDNPVLTARFMVVESL
                     VQINCTVSPITDISYKKDYQQMELSIDYPDYAINQPSTELKVIVQQNRRTDNQAVLTQ
                     PSFYEQHKINYKLNKNLIFEAGNEYREFDISSTRVISRQIESIAYFKPYYHATLYPDI
                     IKSNKEYEYWQDIAGRYIINVQNSDYSETEGDYFFTHFSLAMEIPYLSGEIYILGDIT
                     GNRFDDNSRMQYNYQTGSYEKTLLLKQGGYDYMYAFVPFGEKKGSTKEIEGNKWETDN
                     EYIVYVYHHPFGSIYDRLIGYQIVYSNQYKRE"
     gene            3677..4075
                     /locus_tag="PK641_01550"
     CDS             3677..4075
                     /locus_tag="PK641_01550"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HPG55074.1"
                     /translation="MKNPDFELKFKVRDYECDLQGIVNNANYQHYMEHTRHEFILTKG
                     ISFADLHAKGIDAVIARVEIAYKSPLRSMDEFVSQVRVSIDGVKQIFYHDIYKLPEMR
                     LCVRAKAENVCIVNGELSICDDLNKLLTNK"
     gene            4089..4457
                     /locus_tag="PK641_01555"
     CDS             4089..4457
                     /locus_tag="PK641_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007748119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HPG55075.1"
                     /translation="MKGFYTILLLVVSNTFMTLAWYGHLKFKEFKWFSTLPVIAIILI
                     SWGIAFFEYCFQVPANRVGFKGNGGPFSLLELKVIQEVITLVVFSVFSTLAFKTETLR
                     WNHLVGFIFLTLAVYFVFKK"
     gene            4494..5231
                     /locus_tag="PK641_01560"
     CDS             4494..5231
                     /locus_tag="PK641_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPG55076.1"
                     /translation="MTERILVVDDEEDLCEILQYNLEKAGFIVDVAYSAEEALKQDLS
                     IYRLFLLDVMMGEMSGFKLGSAIKKNDDTANIPIIYLTAKDTENDKLTGFAIGADDYI
                     SKPFSINEVMARVKAVLKRSSQGFLNTKVIQPDLHVFESVADTQNAESIGFDKLILNL
                     TNKTAFIGNKEISLTKKEYELLSLFLQKPDTVLSREQLLNSVWNGDAFVLDRTIDVNI
                     TRLRKKIVPYDKRLTTRQGYGYFFSTK"
     gene            5323..7380
                     /gene="htpG"
                     /locus_tag="PK641_01565"
     CDS             5323..7380
                     /gene="htpG"
                     /locus_tag="PK641_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HPG55077.1"
                     /translation="MKQGNIGVTSENIFPVIKKFLYSDHEIFLRELVSNAVDATQKLK
                     TLSSVGEFKGELGELKVFIKADKKAGTISIVDRGIGMTAEEIDKYINQIAFSGAEEFL
                     DKYKNDANAIIGHFGLGFYSAFMVSKEVEIITKSYKEGSQAVRWSCKGDPNFELEEIE
                     KAERGTEIILHIDDDNKDFLEDSKINTLLSKYCKFLPIPIVFGKKKDWKDGKQIDTDE
                     DNVINNIEPAWVRKPADLKEEDYNKFYKELYPFSEDPLFHIHLNVDFPFNLTGILYFP
                     KVKNNFELQKNKIQLYSNQVFVTDSVEGIVPEFLTLLHGVLDSPDIPLNVSRSYLQSD
                     SNVKKISSHITKKVADSLQEIFKTDRTQFESKWDSLKIFISYGMLTDEKFSERAEKFN
                     LFKNTEGKYFTAEEYKTLIESNQTDKDGNLIHLYTNDAEQQFSYIEAAKSKGYDVLLF
                     DEQLDIHLASKLEQKNEKNRYVRVDADIVDKLILKDDKSTVSLSSDDQQALSEVFRSQ
                     TPKQDKLNFLVTFDAIGADAYPVMITQDEFMRRMKEMAAMQGGMMGFYGSMPDSYNLV
                     VNTDHELVKSLLEEEKSSCSKAVKPKNDEIETLRKKVDELNTANKDKKQDEIPEAEKE
                     IIKTSNERISQLEKEKSEIYSKFADGNDKVRQLIDLALLANNMLKGEALSKFVKRSTT
                     FIK"
     gene            7605..7678
                     /locus_tag="PK641_01570"
     tRNA            7605..7678
                     /locus_tag="PK641_01570"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7639..7641,aa:Arg,seq:acg)
     gene            7761..8231
                     /locus_tag="PK641_01575"
     CDS             7761..8231
                     /locus_tag="PK641_01575"
                     /inference="COORDINATES: protein motif:HMM:NF019749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HPG55078.1"
                     /translation="MKRILFATLILFALSGCSNNAPVNSNIPYASVSFAIDVAVSGAD
                     NELREGMLGNSKIYTTTHPATLSTSFGRYGYSGVVVVRAYDDRLYAFDICCTNEVDRN
                     IALESDGFFLKCPKCGSTFEIGNGYGIVNQGPAIQPLKNYKVTGYSGDRYQVSN"
     gene            8263..8805
                     /gene="hpt"
                     /locus_tag="PK641_01580"
     CDS             8263..8805
                     /gene="hpt"
                     /locus_tag="PK641_01580"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HPG55079.1"
                     /translation="MKNIRIIDKNFEILYNSEDIQKKLSDIAKAINKDYKDKETPLFL
                     CMLSGAYIFAADLLRQIKIPCEISFIKWSSYSGTESTGIIKEQMLPTIDVKGRDVIII
                     EDIVDTGVTIYNLSNKLKQLGAKSSRIATLLTKPSKLKVSIKLDYVGFEIADRFVIGY
                     GMDYNEYGRNLNAIYQLTEI"
     gene            8812..9387
                     /locus_tag="PK641_01585"
     CDS             8812..9387
                     /locus_tag="PK641_01585"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HPG55080.1"
                     /translation="MLNVVIFGAPGSGKGTQSDLIVKKYGLVHLSTGDLLRAEIEKGT
                     KTGKLANELISKGSFVPDDVIIEILNKNIDLYPTAKGFIFDGFPRTVVQSEALDSMLK
                     KRNIKVNVMLNIDVKTDELVKRLLIRGKTSGRSDDNLETIQKRIRIYEEKTLPVMQFY
                     DRQGKHNRIKGDGTMDEVFAEICTAIDKTKA"
     gene            9393..10529
                     /gene="obgE"
                     /locus_tag="PK641_01590"
     CDS             9393..10529
                     /gene="obgE"
                     /locus_tag="PK641_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPG55081.1"
                     /translation="MSGSNFVDYVKIFCRSGKGGAGSMHLRHEKFVAKGGPDGGDGGR
                     GGHVILHANSNYWTLLHLKYARHVFAGDGENGGKSRSFGKDGKDKIIEVPCGTVVYDG
                     ETGEYICELTDDNQSVILLKGGRGGQGNWHFRTSTMQTPRFAQPGEPQIERTIILELK
                     VLADVGLVGFPNAGKSTLLSVVSAAKPKIANYAFTTLEPNLGIVAYRDNKSFVMADIP
                     GIIEGAHEGKGIGLRFLRHIERNAVLLFMIPADTDDICKEYDILINELEQYNPELLDK
                     GRVLAITKSDLLDDELECAISKNLPDIPTVFISSVSGKGIDTLKDLLWNELDKDIVMS
                     MTHRSMDVKIIAQEDKLDSSIDFDLDNDKEKEEFDDDWFGEEDL"
     gene            10529..>11839
                     /locus_tag="PK641_01595"
     CDS             10529..>11839
                     /locus_tag="PK641_01595"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPG55082.1"
                     /translation="MLDRSKKPNIIFENNISVPKTDKTTFSNGIDCYILPSTQEQICR
                     IDLVIKTGKQDHENPLVPVFTSAMLKEGSKDYTANDIAERIDFHGAILVTNTNVSHTV
                     ISLIALEKHVNKLLSIVRSIIFYPTFPEDRLKCLLDKGIAQFKTEQQKVNLISNQQLR
                     ESIYGKNHPYSSHKELAYYEISSDMLNDFHSKNYCSNNCTIVLSGFVNDSLVKYIEQT
                     FGSEAWNKKNIIEHTWLSETPKTGCIRIHHEGSLQTGISIGQLSAPNNHPDSYSMQIL
                     TCVLGGYFGSRLNQSIREEKGYTYGISAHIASYLNKAHFSINTRTKNEVAIETVAAIH
                     DEINNLLQYPVSDEELQTVRNLYITNIGTAFEHPLYISDTLIGLLANNLDFSYYTRFN
                     QVLKTITPNELLNTAQKKRVKTKWRRIKIKFKKKKKTKKKKKKPK"
BASE COUNT         4271 a         2014 c         2045 g         3509 t
ORIGIN      
        1 catcagttaa gttaattgtt gaaccggttg cagttgccag ttgaccaaga ggtgttgctc
       61 ctgtaaatcc atcaactaat tgcccttcac ctattccaca tacatcgtcg gtacgtatcc
      121 aaacactatc tcctgacatt tttgttggat atgcgaagaa taaaaataca cgtgtaatta
      181 atgctacatt taatatattc attccggtac ctccgaaaat ttctttagca aatattacag
      241 caaaggcagt tgcaattgcc ataatccata atggacaatc aacaggaact atcataggaa
      301 ttaaaaatcc tgttacaagg aatccctcat gaatctcttc acctttccat tgtgctacgg
      361 taaattctat acccaaacca actgcatatg ctacgatgat tttaggtagt acagataaaa
      421 agccatacca gaacataggg aaaaagcctg ttgttgccaa ctgaccaata gacaagaagt
      481 gttggtatcc tatattgtac ataccaaaca agagagcggg aatcaaggcg atcactacca
      541 ttatcataat acgttttgaa tcaacagcat catgaatttg ggcacctata ggctttgatg
      601 tttcgttcgg cacgaacaag aaagtttcaa agccgtcgaa cacagagcgg aacatcgata
      661 atttgcctcc ctctgagaag gtaggcttaa ttttatcgat taagtttctt aacgcattca
      721 ttttattttg ttttctaatt ttcaattaac acatttcttt acgcaacata tccaaacctt
      781 cacgcactat gcgttgtagt tccattttag aagagcatac aaattcggca gctgcaaaat
      841 cttcaggaat cacttcataa atgcccaaag cttccatttt atcgatatct cctgctaata
      901 ttgcttttat caaatattca ggatatatat ccattggcat taccttatca tattcaccgg
      961 acattatcat ttgtcgatgt ccgcctaaaa tacgagtatc aaaattgaag gttttcttag
     1021 gacaaagttt catcaaaaat tttgtaaaga aagaaccact ttcgctgtat ttatcaaaac
     1081 gaggcattat ccaaccaata aattcatgct cgtctttacc atctttcaaa gcggtaattt
     1141 gtgatacgaa cggactcaac caagcatcaa cagttgtctg ttttcccgaa agaggattac
     1201 ctgaaataat acgagcagtt gattcgtata catttgcttt tagaatttgt gctaaggatt
     1261 cacccaaaat cattttgtaa taagctttct gatcaacttc cgttcctgca agtactatag
     1321 ttctagaaaa atcaagaagg cctttaccta caaaacgtcc tatgcacaca acatcttgag
     1381 ttgttattgt ccaaacgata tcgcctttat taataggatt aactttatta atttgaacac
     1441 ccacattaca tgaaggataa tttccttcaa atgtagtaac tgtagcattt tttacatgag
     1501 agaatgttgt tgttccattt ttgattccaa cataaacagg tgcaatttta gacaatgcat
     1561 ctattccgtt ttgaaaatcg gattgttcgt ttttaaagat aaaatcaaaa tccggagaaa
     1621 gaggggcgtt atcaaaagta gaaataaaaa tagctttagg agtatcatca ggatttgcca
     1681 taacgtcgta aggacgttgt ttaataaagg caaacatacc tgcatccgat agtgattgtt
     1741 taatttcatc tgctgaagaa tttaaatcaa cagcctttat actttcttgc ttcattttga
     1801 aatcgttttt aactacgatg tccaaaattt tgcgacgttc accgcgatta actgccacca
     1861 actcgccgct aacaggagaa acaaccttta aattagggtg attcttgtct acgaataata
     1921 ctgttcctat cttcacggca tcacccggtt tgagaaccgg ctttggcacc ataccataaa
     1981 aatcatcggg attaaaccct atcatttccg attgtgatgc tcgaccatag actaattcag
     2041 atgctccatc gagtcgaatg tcaaggcctt tctttaattt gattacacct gccataaaat
     2101 gttatatatt agtgtctttt taaaaaactc cgcaaaatta ctttttttat tccacaatac
     2161 aatgtatttt catcgaaaaa aacagcaaaa ttgagagttt ttttgaaatc ttgaatccct
     2221 caaaaataca catggaaata cataaaaaac aagcaataaa cttagatttt attacacatt
     2281 atgaatagat ttgtgcaatt agtgtattaa ttatttgaga tctcaattat agccctaaat
     2341 attattcctt tcagccaaat tttataactt tgcagcatga aaaaattcag cttgatatat
     2401 attgcctgca tgatacttac atacagtttt gcagacaata ttgtatacga aacaaaatct
     2461 ctgaacaaaa acatcaaaac agttcagatt gaaaactttt cacggaagcc atactaccct
     2521 attatagagt taggcggaaa agataaagtt acattgtcgt ttgatgactt atcaccggag
     2581 ataaatacct actcttacag tattattcat tgcaatgcag attggacaaa atctaagcta
     2641 aacgaatttc agtatttgaa tggtttcgat ggagggttta ttagaaatta cgagagctct
     2701 gtcaatacat attttccata tacgcattat acgcttacat ttccaaacga agatgtaaca
     2761 tgtactgtat caggcaacta tattatttca gtatatcctg ataacgacaa ggataatcct
     2821 gtgctaacag ctagattcat ggtagttgaa agtttggttc aaattaattg cacggtaagc
     2881 cctatcacag acatcagtta taaaaaagat tatcaacaaa tggaattatc cattgattat
     2941 cctgactatg caattaatca gccgtcaacc gaacttaaag ttatagtgca acaaaatcgg
     3001 cgcacagata atcaagccgt gcttacacaa ccgagctttt acgaacagca taaaataaat
     3061 tacaaactca acaagaatct tatttttgaa gccggaaacg aatatcgtga gtttgatatt
     3121 tcttctacac gcgtaattag tagacaaatt gaaagtatcg cttatttcaa accatattat
     3181 catgctacct tgtatcctga tattataaaa tcaaataaag aatacgaata ttggcaagat
     3241 attgccggca gatacatcat caatgttcaa aactctgatt attcagaaac agaaggagat
     3301 tatttcttta ctcatttttc tctagctatg gagattcctt atttaagtgg ggaaatttat
     3361 attttgggtg acatcacagg aaatagattt gacgataatt ctcgaatgca atacaattat
     3421 caaaccggtt catacgaaaa gactttactt ttaaaacagg gcggatatga ttatatgtat
     3481 gcttttgttc cattcggcga aaaaaaagga agtacgaaag agattgaagg aaacaaatgg
     3541 gaaactgata atgaatatat agtttatgta tatcatcatc cattcggcag tatttacgac
     3601 cgtttaatag gatatcaaat agtttattct aatcaatata aaagagaata aattgtaatt
     3661 tagttttaaa attaacatga agaatcccga ttttgaattg aagtttaagg tgcgcgacta
     3721 cgaatgcgat ctgcaaggaa ttgtaaataa cgccaactac caacactata tggaacacac
     3781 aaggcacgaa tttatactca caaaaggaat aagttttgcc gacttacatg cgaaaggcat
     3841 tgatgcagta attgcacgtg tagaaatcgc atacaaaagc cccttacgca gtatggacga
     3901 atttgtatcc caagttcgtg tcagcattga tggagttaaa caaatctttt atcatgacat
     3961 ttataaattg cctgaaatgc gactttgtgt tcgtgcaaaa gctgaaaatg tatgcatagt
     4021 caacggagaa cttagcattt gtgacgattt aaacaagtta cttacaaaca aataaacagt
     4081 aaaataaaat gaaaggtttt tataccattt tattgttagt agtgtcaaat acctttatga
     4141 cattggcttg gtatggtcat ctcaaattca aagaattcaa atggttttca actctacctg
     4201 taattgcaat aattttgatt agttggggca tcgcattttt tgaatactgt tttcaggtac
     4261 cggcaaacag agttggtttt aagggcaacg gaggtccatt ctcattactt gaactaaagg
     4321 tgatacaaga agttatcaca cttgttgtat tttcagtatt ttctacactc gcatttaaaa
     4381 cagaaacttt gagatggaac cacttagttg gttttatatt cctaacttta gcagtatact
     4441 ttgtttttaa aaaataatat atacttttgc taataaaaca atatcaaaac accatgacag
     4501 aacgcatcct agttgttgac gacgaagaag atttgtgcga aatacttcaa tataatcttg
     4561 aaaaagccgg ttttattgtt gatgtagcat attctgcaga agaagcactt aagcaagatc
     4621 taagtattta ccgcttattc ttgcttgatg ttatgatggg tgaaatgtca ggatttaaac
     4681 ttggatctgc catcaaaaaa aatgatgata cagccaatat acctattata tatctaacag
     4741 caaaggatac cgaaaacgac aaattaacag gatttgcaat tggagctgac gattatatat
     4801 ccaaaccttt ttcaataaat gaagtaatgg ctcgtgttaa agccgtgtta aagcgtagtt
     4861 cgcaaggttt tttaaatact aaagtcatac aacctgattt acatgtattt gagtctgttg
     4921 cagacacaca aaatgctgag agtataggat ttgacaaact aattctaaat ctcacaaata
     4981 aaacagcatt tattggaaat aaagaaattt cactaacaaa aaaagaatac gaactattat
     5041 ctctttttct acaaaagcct gacacagtac tatctcgaga gcaattactc aatagtgtat
     5101 ggaatggtga tgcctttgta ttagatcgta caattgacgt aaacatcacc cgactaagaa
     5161 aaaaaatagt accatacgac aaacgtctaa ctacaagaca aggatatggt tatttcttct
     5221 ctacaaaata gctgacaaaa gtgtcttttt gtcagtctct attgtttggc atcttttttg
     5281 ccctttatag gcaaaataat aaccaaaaca attatataag atatgaaaca aggtaatatc
     5341 ggagtaactt cggaaaacat ttttcctgtt atcaagaaat ttttgtacag tgaccacgag
     5401 atttttctac gcgaattagt atcaaacgct gttgatgcca cacaaaaact aaaaacccta
     5461 tcatctgttg gcgaattcaa aggcgaatta ggcgaactta aagtatttat aaaagccgac
     5521 aagaaagctg gaaccataag cattgttgat agaggtatcg gtatgactgc agaagaaatt
     5581 gacaaataca ttaatcaaat cgcattttca ggcgcagaag aatttttgga taaatacaaa
     5641 aatgatgcaa atgccattat tggacatttc ggattaggtt tttattctgc tttcatggtt
     5701 tcaaaagaag ttgaaatcat tacaaaatca tataaagaag gtagtcaggc tgttcgttgg
     5761 agttgtaaag gagatcctaa ttttgaattg gaagaaattg aaaaagctga acgtggaaca
     5821 gaaatcattc ttcatattga tgatgacaac aaagatttct tggaagatag taaaatcaac
     5881 acacttttaa gcaagtattg taagttctta cctataccta tagtatttgg caagaaaaaa
     5941 gattggaaag acggtaaaca aattgatacc gatgaagata atgtaataaa caacatagaa
     6001 ccggcatggg ttcgcaaacc tgctgactta aaggaagaag attacaataa attttataag
     6061 gaattatatc cattttcaga agatccccta ttccatatac atcttaatgt agattttcct
     6121 tttaatttaa ccggtatatt gtatttccct aaggttaaaa acaatttcga actgcagaag
     6181 aataaaatac aattatatag caatcaggta tttgtaactg attcggttga aggtatcgta
     6241 cctgaattct tgacattatt gcatggtgta ttagattctc ctgatattcc gttaaatgta
     6301 agccgttctt acttacagag cgattctaac gttaagaaaa tttcgagtca catcaccaaa
     6361 aaagttgctg atagcttaca agaaattttc aagacagatc gtactcaatt tgaatcaaaa
     6421 tgggatagct taaagatctt tatttcttac ggaatgctta cagatgaaaa atttagtgag
     6481 cgggctgaaa aatttaattt attcaaaaac actgaaggca aatattttac agccgaagaa
     6541 tacaaaactc taattgaaag caatcaaact gacaaagacg gcaatttgat acatctttac
     6601 actaatgatg cagagcaaca attcagctat attgaggctg caaaatctaa aggatatgac
     6661 gttttgttat ttgacgaaca attagatata catttagcat ctaaattgga acaaaaaaat
     6721 gaaaagaatc gttatgttcg tgttgatgcc gatatagttg ataaattaat tttaaaagat
     6781 gacaaatcaa cagtaagcct atcttctgac gatcaacagg cattatcaga ggtattccgt
     6841 tcacaaactc cgaaacaaga caaacttaat ttcttggtaa catttgatgc tattggagct
     6901 gatgcttatc ctgtaatgat tacacaggat gagtttatgc gccgtatgaa agaaatggcg
     6961 gcaatgcaag gtggaatgat gggattttac ggaagtatgc ctgattcgta taatttagtt
     7021 gtcaatactg atcacgagct cgtaaaatca ttactagaag aagaaaaatc ttcatgttca
     7081 aaagcggtta aacctaagaa cgatgagatc gaaactcttc gtaaaaaagt tgatgagttg
     7141 aatactgcca acaaagataa aaagcaagac gagattcctg aggctgaaaa agaaatcatc
     7201 aaaacaagca atgaacgaat cagtcaactc gaaaaagaaa aatctgagat atatagcaag
     7261 tttgccgatg gtaacgacaa agttcgtcaa ttaattgacc ttgcactact tgctaacaat
     7321 atgcttaaag gcgaagctct aagcaagttt gttaaacgta gtaccacttt tataaaataa
     7381 tttcgtttcc ataattcaaa aatcttcgag atgagctata atatagtttt tctcggagat
     7441 ttgcgaatta atacttataa aacaattgca gataattttt gtgcttttat agtaataaca
     7501 agtagaaaaa acgcaaaaac ctgaactatt tttttgtaaa taagaaaaaa cattatacct
     7561 ttgcagccac gtaaaaaaag gagttttaat aaaaactacc gaatggcttc gtagctcaac
     7621 tgaatagagt atcacactac ggatgtgagg gttccgggtt tgaatcccgg cgaggtcact
     7681 taaaagtaag caccgatatt aaagtaacat ttatatcggt gtttcttttt aaataagata
     7741 atctaatgta accttaagat atgaaacgta tactatttgc aacattgatt ttattcgcac
     7801 ttagcggatg ttcaaacaac gcacccgtaa atagtaacat cccatacgca agcgttagct
     7861 ttgcaattga tgttgcagta tccggtgccg acaacgaatt acgagaagga atgcttggta
     7921 atagcaaaat ctatacaact acacatcctg ccacactatc aacctcattc ggacgctacg
     7981 gatatagtgg cgtagtagta gttagagcat acgacgaccg attatacgcc ttcgatattt
     8041 gctgcacaaa tgaagtagac cgtaacatag cacttgaatc agatggattc tttttaaaat
     8101 gtccaaaatg cggttctaca tttgaaatcg gaaacggata cggaatagta aatcaaggcc
     8161 ctgccattca accgttaaaa aactacaaag taaccggata ttcgggcgat agataccaag
     8221 taagtaatta attttttact aactttggga gattttttta gcatgaaaaa cattcgtata
     8281 attgataaga actttgagat tctctacaat tcagaagaca ttcaaaaaaa attgtctgac
     8341 atagctaaag ccattaataa ggactataaa gacaaagaga ctcctttgtt tttatgcatg
     8401 ctttcaggcg catatatatt tgcagcagat ttgctcagac aaataaaaat accctgcgaa
     8461 atatcattta ttaaatggtc ttcgtattcc ggaacggaaa gtacaggtat tatcaaagaa
     8521 cagatgttgc ctacaattga tgtaaaaggc agagatgtaa ttattataga agacatagtt
     8581 gacacagggg tcactattta taatttgtca aacaaattaa aacagttagg tgcaaaaagt
     8641 tcaagaatag ccactttact tacaaaaccg agtaaactta aagtatcgat taaattggat
     8701 tatgtaggtt ttgaaattgc agacagattt gttattggat acggaatgga ttataatgaa
     8761 tatggcagaa atttaaatgc catttatcaa ttaactgaaa tatagatttt tatgcttaat
     8821 gtggttatat ttggtgcccc aggttccggt aaaggaacac aaagcgattt aatcgtaaag
     8881 aaatatggtt tagtacacct atctacagga gatttgcttc gcgctgaaat agaaaaaggc
     8941 actaaaacag gaaaattagc aaacgaatta atatcaaaag gatcatttgt tcctgacgat
     9001 gttattatcg aaatactaaa taaaaatatc gatttatatc ctactgcaaa aggatttatt
     9061 tttgatggat tcccacgtac agttgttcaa tcagaagcat tagactctat gttgaagaaa
     9121 cgcaacataa aagtaaatgt gatgctaaat atagatgtta aaaccgatga attagttaaa
     9181 cgtctattaa tcagaggtaa aacatcaggc agatctgatg ataatttaga aacaatacaa
     9241 aagcgcattc gtatatacga agaaaaaacc ttgcctgtta tgcaatttta cgatcgtcaa
     9301 ggcaaacata atcgcatcaa aggtgatgga actatggatg aggtatttgc agaaatatgt
     9361 accgcaattg acaaaacaaa agcttaaaca aaatgtcagg atccaatttc gtcgattatg
     9421 tcaaaatttt ttgccgctcc ggcaagggtg gtgcaggttc tatgcacttg cgtcacgaaa
     9481 aattcgtagc taaaggaggt cctgatggtg gcgatggagg acgtggagga catgttattt
     9541 tgcatgcaaa ctccaattac tggacacttt tacacctaaa atatgcacgt cacgtatttg
     9601 ccggagatgg tgaaaatggc ggcaaaagtc gtagctttgg aaaagacggt aaagacaaga
     9661 taatagaagt tccttgtggc acagttgttt atgatggaga aacaggtgaa tacatttgtg
     9721 aattaacaga tgacaaccaa agcgttattc ttttaaaagg aggacgagga ggtcaaggaa
     9781 attggcactt cagaacatca acaatgcaaa ctccacgatt tgcacaaccg ggagaaccac
     9841 aaattgaacg tacaataatc ttagaattaa aagttctagc tgacgttggc ttagtcggat
     9901 ttcctaatgc gggaaagtct acattattat ctgtcgtttc agcagccaaa cccaaaattg
     9961 ctaattatgc atttactaca ttagaaccaa acctgggtat cgtggcatat agagacaata
    10021 agtcattcgt catggctgac attccgggta taatagaagg agcacatgag ggcaagggta
    10081 ttggattgcg atttttacgt catattgaac gcaatgctgt attactattt atgataccgg
    10141 cagacactga cgatatttgc aaagagtatg atattctaat aaatgaatta gaacaataca
    10201 atcctgaact attagacaaa gggcgggtat tagccattac aaaatctgat ttattagatg
    10261 acgaattgga atgtgccatc tctaaaaacc ttcctgacat acctactgtg tttatatcat
    10321 ccgttagtgg caaaggaata gacactctaa aggacttact ttggaacgaa ttagacaaag
    10381 acattgttat gtcaatgact cacagatcaa tggacgtgaa gattattgca caagaagata
    10441 aacttgactc gagtatagat tttgacctcg acaacgataa ggagaaggaa gaatttgatg
    10501 atgattggtt tggtgaggag gatttataat gttagatcgt tcgaaaaaac caaatatcat
    10561 ttttgaaaat aatatttcgg ttcctaaaac cgacaaaact acattttcaa atggtataga
    10621 ttgctacata cttccaagca cacaagaaca aatttgtcgc atcgatttgg tgataaaaac
    10681 gggtaaacaa gaccacgaaa accctttagt accggtattt acatcagcca tgttaaaaga
    10741 aggcagtaaa gactatacag ccaacgacat tgccgaacga atagacttcc atggtgctat
    10801 attagttaca aataccaatg tttctcatac agtaataagc cttatagcat tagaaaagca
    10861 cgtcaacaaa ttattaagta ttgtacgctc aattattttt tatccgacat ttcctgaaga
    10921 tagactaaaa tgcttattgg ataaaggaat cgcacagttt aagacagagc aacaaaaagt
    10981 taatctgatt tcaaatcaac aattgcgcga atcaatatat ggaaagaatc acccatatag
    11041 ttcacacaaa gaacttgcat actacgagat aagtagcgat atgcttaacg attttcactc
    11101 taaaaattac tgctcaaata attgtacaat agtattatca ggctttgtaa acgattcgct
    11161 tgttaaatac attgagcaaa catttggttc ggaagcatgg aataaaaaaa atataattga
    11221 acatacttgg ttaagcgaaa ctccaaaaac agggtgcata cgcatacacc atgaaggttc
    11281 tttacaaacc ggaatttcaa taggtcaatt gtcggcaccc aacaatcatc ctgactccta
    11341 ctccatgcaa atattaacat gtgttttggg aggctacttt ggcagcagac ttaatcagag
    11401 catcagagaa gaaaaaggat acacatacgg catatctgca catatagcat catatctaaa
    11461 caaggcacat ttttctatta acacgcgtac caaaaacgaa gttgcaattg aaactgtagc
    11521 tgcaatacat gacgaaataa ataatttact gcaatatcct gttagtgatg aggagctaca
    11581 aactgttaga aacctgtata taactaacat tggtaccgca tttgagcatc cgctatatat
    11641 atcagatact ttaataggac tactggcaaa caatttggat ttttcatatt ataccagatt
    11701 taatcaagtt ttaaaaacta taactcctaa cgagctgcta aacacggccc aaaaaaaacg
    11761 ggtaaaaacc aagtggcgaa gaataaaaat aaaatttaaa aaaaaaaaaa aaacaaaaaa
    11821 aaaaaaaaaa aaacctaaa
//
LOCUS       DAOPID010000021        11642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_6627_length_11642_cov_4.664969, whole genome shotgun sequence.
ACCESSION   DAOPID010000021 DAOPID010000000
VERSION     DAOPID010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 11642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11642
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6627_length_11642_cov_4.664969"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PK641_01600"
     CDS             <1..614
                     /locus_tag="PK641_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HPG55083.1"
                     /translation="GSLGGVARKTRETIVLCEAAGFNKVFVETVGVGQSETAVHSMVD
                     FFLLLQITGAGDELQGIKRGIMEMADGIIINKAEGNNIERANLAQAQYINALHLFPAH
                     ESGWKPQVLKCSAFQKTGIEEIWNMIEEYTQFTKGNDYFAHKRRSQTKYWMYETIDGK
                     LKSNFYSNPIISAKLSQLEQQVMKDEVSSFIAAHKLLEEYFKM"
     gene            complement(619..1092)
                     /locus_tag="PK641_01605"
     CDS             complement(619..1092)
                     /locus_tag="PK641_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005790644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HPG55084.1"
                     /translation="MAQIQLDQLDEKILQLVSQNARTPFLEVARECNVSGAAIHQRIQ
                     KLTSQGVIKGSEFIIDPVKVGYHTCAYMGLFLKDANAFEGVVSALKKIPEIVECHYTT
                     GKYAMYLKIYAKDNAHFLSIIHDKIQVIEGIASTETLICLGEAFRRQITVECDKE"
     gene            1262..1963
                     /locus_tag="PK641_01610"
     CDS             1262..1963
                     /locus_tag="PK641_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPG55085.1"
                     /translation="MAKHQSLSPKNIIETSSLWNVLTAEQKVYLAHNFQITTFKKNAV
                     IYSENEQPNSLLCVISGKVKIYKEGNGGRSQIVRIIKPEEFFGYRALFAGEKYVTTAA
                     AFEACVIYEIPEPVILNLIKNNADLAFSFIQFLSIDLGISDARSVVLTQKHIRGRLAE
                     SLIHIKEHYGTEENDKTLSLKLSREDLANLSNMTTSNAIRTLSLFSKEKLIAIEGRKI
                     KIIDEERLRRISKFE"
     gene            complement(1967..2641)
                     /locus_tag="PK641_01615"
     CDS             complement(1967..2641)
                     /locus_tag="PK641_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HPG55086.1"
                     /translation="MIFGFFLFLMILSYIVQYRLKSKFKKYSQMPIAQGVTGKDIAEC
                     MLQANNIKDVKVFSTSGRLTDHYNPANKTINLSEDVYGTSSVAAAAVAAHECGHAVQH
                     AESYAWLQFRSAIVPVISFASSWMQWVLLAGILLIEVFPYLLVVGVVLFAMTTLFSFI
                     TLPVEINASKRALAWLSTSGLTTSANDEAAKDALKSAAYTYVVAALGSLATLLYYVMI
                     LLGRRD"
     gene            complement(2692..3969)
                     /locus_tag="PK641_01620"
     CDS             complement(2692..3969)
                     /locus_tag="PK641_01620"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HPG55087.1"
                     /translation="MAKVDVLLGLQWGDEGKGKVIDVLTPNYDVVTRFQGGPNAGHTL
                     EFNGEKYVLRSIPSGVFQGDKINIIGNGVVLDPALFKAEADNLAASGHDLTKRLYISK
                     KAHLIMPTHRTLDAAGEAAKGASKVGTTGKGIGPTYTDKVSRNGLRVGDIINNFAEKY
                     ATAKAKHEQMLQSMGFEYDITAAEKEWFEGIEYIKKFNLIDSEHVINGLIEDDKAILC
                     EGAQGTMLDVDFGSYPFVTSSNTICAGACSGLGVAPNKIGDVYGIFKAYCTRVGSGPF
                     PTELFDETGDIICEKGHEFGSVTGRKRRCGWIDLVALKYAIMLNGVTKLIMMKGDVLD
                     MFETIKACVAYKIDGEELDYFPYEICDKKIEPVYKELKGWKTDMTKMHKESEFPREYN
                     DYLDFLEDELNVPIRIVSVGPDRDQTIVRYLAE"
     gene            4146..4313
                     /locus_tag="PK641_01625"
     CDS             4146..4313
                     /locus_tag="PK641_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPG55088.1"
                     /translation="MAHVISEDCIACGTCISECPVEAISEGDIYSINPDICTDCGTCA
                     EACPTGAIHPA"
     gene            complement(4387..4758)
                     /locus_tag="PK641_01630"
     CDS             complement(4387..4758)
                     /locus_tag="PK641_01630"
                     /inference="COORDINATES: protein motif:HMM:NF023109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HPG55089.1"
                     /translation="MINLFNRQPKEEPKVQREEREDKEIVFSKTVKAGKRVYYLDVKK
                     DRRDDLFLSITESKRKAVTEDGQFVFEKHKIFLYKEDFDKFEDAMTEALNFMRKAMQT
                     SYNNFEKEQNSDSIESKDDAE"
     gene            4954..5907
                     /gene="nusB"
                     /locus_tag="PK641_01635"
     CDS             4954..5907
                     /gene="nusB"
                     /locus_tag="PK641_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPG55090.1"
                     /translation="MINRVLIRVKTVQLLYANLNNPSSSQVSAENELQTSLDKTYELY
                     HWLLQLSVDITSYAVKRIEIGLNKMRPTPEESNPNRRFINNKFAKQLAKNKELAKYVS
                     EHGISWEENNDLVKNLYELICRSDIYKEYMAAPSSDYENDKVFWRRVYKKILMPDKSL
                     DAQIEEINLYWNDDSETVFSFIDKTIKHFCVENEENQSLLPMYRDESDKEFAIQLYKF
                     TIENKDEYMKWIEDTAKNWEVERIAAMDIAIMQAAIAELTHFPTIPVNVTLNEYIEIS
                     KFYSTEKSCTFINGVLDSITNKLRKENKLLKVGALVKDDSK"
     gene            5926..6255
                     /gene="yajC"
                     /locus_tag="PK641_01640"
     CDS             5926..6255
                     /gene="yajC"
                     /locus_tag="PK641_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HPG55091.1"
                     /translation="MNLTVLLQATAATEGGMGQYGSIIMLVLIFVVFYFFMIRPQMKK
                     QKEIKKFRDSISVGDKVITAGGIYGKIKEVKDNTIILEIADNVRITVDKGSVFATAAD
                     SATAVAK"
     gene            6255..7256
                     /locus_tag="PK641_01645"
     CDS             6255..7256
                     /locus_tag="PK641_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55092.1"
                     /translation="MSKKEQIRVFSNFIKKVNAFLFSKNVFIFLFFLIISYVFWFIIS
                     LNKNYETRLTFPLEYRNIPKEIALSTDLPSSIDVKVKDKGTVIFTYKQSKFSALVIDF
                     KDIPGFSKNKSAVLSTSDTFDKKIKEQLAQTTVITDYFPSEITIERIFLSSKKLPIRI
                     NCDIRFAKQYNLSDSIKANPDSIILYGTEECLDTTVYISTNLLRIKDIKDTLNRSVGL
                     LLPKHTKADNSKVNLMIPVEVSTEASIMIPVKVINTPKNIRVRVLPGEVKLTYMVGIS
                     KYKKVKSSDFTVSIDYEDLIESSSKTEFVNIDTKPSFVSNCRINPQEVQWVMEVIED"
     gene            7262..7846
                     /gene="coaE"
                     /locus_tag="PK641_01650"
     CDS             7262..7846
                     /gene="coaE"
                     /locus_tag="PK641_01650"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPG55093.1"
                     /translation="MIIGVTGGIGSGKTHFCHLMEIAGFPVFYSDTESKKLLNSDPSI
                     KAQIIDLFGASAYSEGVLDSKHIAQQVFSNKKLRTKLNNIVHPAVKKIFDLWLIEHES
                     SPIILLESAILFESGFDAFVDKIIVIAAPIETRIKRVVQRDKCEEKDVLSRMKSQMSD
                     EIKISKANFVIQNGEHDDLNKQIFDLLGKLMSPK"
     gene            complement(7872..8420)
                     /gene="def"
                     /locus_tag="PK641_01655"
     CDS             complement(7872..8420)
                     /gene="def"
                     /locus_tag="PK641_01655"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPG55094.1"
                     /translation="MIYPIVIYGNPVLKKVALPVPADYSNLEQLISDMFQTLEKADGV
                     GLAAPQIGLSLRLFVVDLHLMADEIPSLATFKKTFINAQIISYSEDDSLKEEGCLSLP
                     GISESVRRPNSITINYYDENWNEKTETFSGFAARAIQHEYDHIDGHVFVDKVSLIRRQ
                     MLSSKLVAMTKGKFNCRYKFKM"
     gene            complement(8417..8839)
                     /gene="ruvX"
                     /locus_tag="PK641_01660"
     CDS             complement(8417..8839)
                     /gene="ruvX"
                     /locus_tag="PK641_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HPG55095.1"
                     /translation="MSRILAIDYGKKRVGIAVSDPSQLIAGGLTTVDSQQILTFLAKY
                     LATESVECIVVGLPKQMNNMPSESMKYITPFVNSLKNKFPNVRVEFFDERFTSVLAHK
                     TMLTGGLKKKDRQNKALVDEISATIILQDYMESQRMKI"
     gene            8901..9929
                     /gene="galE"
                     /locus_tag="PK641_01665"
     CDS             8901..9929
                     /gene="galE"
                     /locus_tag="PK641_01665"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HPG55096.1"
                     /translation="MKILVTGGTGYIGSHTVVELQNEGFEPIIIDNLSNSSISVLEGI
                     EKITGKRPVFEQIDCKDINDMDLLFTKHKDIVAIIHFAASKAVGESVIKPLMYYRNNL
                     KSMLNIMELMIKHNVENLVFSSSCTIYGQPDILPVTEKAEIKPALSPYGNTKQICEEI
                     INDTAHANPHINAILLRYFNPIGAHESSEIGELPIGVPQNLIPYVTQTAAGIRKQLQV
                     FGNDYNTPDGSCIRDYINVTDLAKAHVASVKRLLDKKGKSSVEAFNIGTGRGTTVLEV
                     ISLFEKVTGVKLNYKIVGRREGDIEKVWADASLANNELGWRAEKSMEDTLLSAWKWEQ
                     HLKQTKTR"
     gene            9926..10987
                     /locus_tag="PK641_01670"
     CDS             9926..10987
                     /locus_tag="PK641_01670"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG55097.1"
                     /translation="MKKRLFLMGICIFATSLVGSAKKAVVVSTLQLPPAEAIYNEGIN
                     LLRQKQYDDAIIKFTATIAEDTTFTKAYYNRAIANFESERYNKAMNDLNVAIRQANNE
                     TASECYLLRGKIMFHLDDIKNALTDLDKAIELNPSNINARLDKAALLQYKREYALAEE
                     EYSNILNISPDNASALLERGNCRQYIGQLEAAMNDYKILAINDSSNFQAKLNIATLTW
                     KISADTVNSISLLNKLAKDNPQSAEVFNARGLIYSLTAQYDSAIADFDKAIAINKNYA
                     AAYNNRGSAYYKQNKLETAIDNYNRAIEINTNYGDAYLNRAIVRELLRDENGSCNDLK
                     AAAKLGVKNASEYFTKQCE"
     gene            10995..>11642
                     /locus_tag="PK641_01675"
     CDS             10995..>11642
                     /locus_tag="PK641_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55098.1"
                     /translation="MKKISIILICTLACNFLFAAKTNIPLSKDSLKSYKTAVKQGDKF
                     YRLQDEKKYQACGQYLTAYNLYAGDASLCYKLGNCYLTGFSKTKAITFFDAVADIDST
                     HKQFPDIYWKLGTAYQLDNQFDNAIVSYQNHKDILSKGKQNKKTIAKIKQTDKRIQEC
                     QSGLSLEKIKNPLFIDNVSAINTSADEYNTICNPNGKTISYTNATLLSSGERREHS"
BASE COUNT         4164 a         2094 c         1853 g         3531 t
ORIGIN      
        1 caggctcgct aggaggagtt gcacgcaaaa ctcgtgaaac catcgtatta tgcgaagctg
       61 caggatttaa caaagttttc gtagaaactg tgggtgttgg tcaatcagaa actgccgttc
      121 actctatggt agattttttc ttgctcttac aaattacggg tgccggtgat gaattacaag
      181 gcattaaacg tggtatcatg gaaatggcag acggtattat tataaataaa gcagaaggaa
      241 ataatataga acgcgcgaat ttggctcaag cacaatatat caatgcactg catttattcc
      301 ctgctcacga gtctggatgg aaacctcaag ttttaaaatg ctccgctttt caaaaaacag
      361 gtattgaaga gatttggaac atgatagaag aatataccca atttactaaa ggaaacgact
      421 attttgcaca caaacgccgc tctcaaacaa aatactggat gtatgaaact attgacggaa
      481 aacttaaaag taatttctac agtaatccaa taatttctgc taagttaagt caactagaac
      541 aacaagtaat gaaagatgaa gtaagttcat ttattgctgc acacaaacta ttagaagaat
      601 attttaaaat gtgataaatt attccttatc acattctaca gtaatttggc gtctaaaagc
      661 ttctcccaaa caaattaaag tttctgtgct tgctattcct tctattacct gaattttatc
      721 gtggataatg ctcaagaagt gtgcattatc tttagcatat atttttagat acatggcata
      781 tttacctgtt gtataatgac attcaacaat ctcaggtatt ttcttcaaag cagaaaccac
      841 accttcaaat gcgtttgcat ctttcaaaaa aagccccata tatgcacatg tatggtaacc
      901 taccttaacc gggtctataa taaattcaga acccttaata accccttgac ttgtaagttt
      961 ctgaatacgt tgatggatag cagcaccaga tacgttacat tcacgtgcca cttccaaaaa
     1021 aggagttctt gcattttggg acactaattg aagaattttt tcgtcaagtt ggtcaagttg
     1081 aatttgtgcc ataataatat aaatacatta gtattgtaag gtaaaatatt ttgctgtttc
     1141 ctgataaaat atcattaaca catacaaaga taataatttt ccaaaaaaaa cagtaaaaaa
     1201 gatgttaata cataaaaata cactaaattt gtgccaagca acatagttat atagcattac
     1261 aatggcaaaa catcagtcat taagtcctaa aaacattatc gaaacatcaa gtttgtggaa
     1321 tgttctaact gccgaacaaa aggtatattt agcacacaat tttcaaatta ctacctttaa
     1381 aaagaacgct gtaatatatt cagaaaacga gcaaccaaat agtttgttat gcgttattag
     1441 tggtaaggtg aaaatatata aagaaggtaa tggaggacgc agccaaattg tcagaataat
     1501 taaacctgaa gaattttttg gatatagagc attatttgcc ggagaaaaat atgttactac
     1561 agctgctgct tttgaagcct gcgttattta cgaaattccg gaacctgtta tactcaacct
     1621 tatcaaaaac aatgcagatc tagcattctc attcatacag tttttgtcta ttgatttagg
     1681 catatcagat gcccgttcgg ttgtgcttac acaaaagcac attagaggac gtttagcaga
     1741 atccctgata cacattaaag aacattatgg aacagaagaa aatgataaaa cactatcact
     1801 taaattatct cgtgaggatt tggctaatct atcaaacatg acaacatcaa atgccatcag
     1861 aactctgtca ttattttcaa aagagaaact gatagcaata gaaggtcgta aaatcaaaat
     1921 tattgacgaa gaacgcctac gtagaataag caagtttgaa taatacttaa tctctgcggc
     1981 caagcaagat cattacgtaa tatagtaatg tagccaatga gccaagtgcc gcaaccacat
     2041 atgtatatgc agcagatttg agcgcatcct ttgcagcctc atcatttgca gaagtcgtta
     2101 aacctgatgt cgacaaccaa gccaaagctc ttttactagc attaatctca acaggcaagg
     2161 tgataaaact aaataatgtt gtcattgcaa ataagactac tccaaccact aataaatatg
     2221 ggaatacctc aatcaacaat atcccggcta ataaaaccca ttgcatccag cttgatgcga
     2281 aactaattac aggtactatt gcagatctaa actgaagcca agcatatgat tcggcatgtt
     2341 gaacagcatg accacattca tgagcagcaa ccgccgctgc tgcaacacta ctagtaccat
     2401 aaacatcctc gctcaaattt atggttttat ttgctggatt ataatggtct gttaatctac
     2461 ctgaagtaga gaacactttc acatccttaa tattattagc ctgcaacatg cattctgcaa
     2521 tatccttgcc tgttacaccc tgtgcaatag gcatctgcga atatttttta aacttagatt
     2581 tcaaacgata ctgaacaatg tagctcaata tcattaaaaa caaaaaaaat ccaaaaatca
     2641 tattaataat attttagtat tatattgtac taattgcaaa tactatgcca attattctgc
     2701 caaataacgg acaatggttt gatctctgtc cggacctaca gaaacaatac gaattggaac
     2761 attaagttcg tcttctaaaa agtccaaata atcgttatat tccctcggaa attcgctttc
     2821 tttatgcatt ttggtcatat cggttttcca acctttaagt tccttatata caggttctat
     2881 tttcttgtca caaatttcgt atgggaaata atctaattct tctccatcaa ttttatatgc
     2941 cacgcatgcc tttattgttt caaacatatc caaaacatca cccttcatca taattaattt
     3001 agtaactcca ttaagcatga tagcatactt aagagcaact aaatcaatcc aaccgcaacg
     3061 tctttttcgt cctgtaacag atccaaactc gtgacctttt tcacaaataa tatcacctgt
     3121 ttcatcaaat aactctgttg ggaatggacc actacctaca cgtgtacagt atgctttaaa
     3181 aattccatat acgtcgccaa tcttatttgg agctacaccc aacccactac atgcacctgc
     3241 acaaattgta tttgaagatg ttacaaatgg atatgaacca aaatcaacat ctagcatagt
     3301 tccttgtgca ccttcacata aaatagcttt atcatcttca ataagaccat ttattacatg
     3361 ctcactatca atcaaattaa actttttgat atattcaatg ccttcaaacc attctttttc
     3421 agctgcggtg atgtcatatt caaaacccat cgattgcaac atttgttcat gttttgcttt
     3481 tgctgtagca tatttttcag cgaaattatt aataatatca cctactcgta aaccattcct
     3541 gcttacttta tcagtatatg taggtccgat acctttccct gttgttccaa ccttagaagc
     3601 accctttgca gcttcacctg cagcatccaa cgtacgatgt gtaggcatga tcagatgcgc
     3661 tttttttgaa atatacaaac gttttgtcaa atcatgtcct gaagccgcta aattatctgc
     3721 ctctgcttta aacaaagcag gatctaaaac gacgccgttt cctataatat taatcttgtc
     3781 tccttgaaaa acacctgatg gaattgaacg taatacatac ttttctccat taaattccaa
     3841 tgtatgtcct gcattaggac caccttgaaa acgagttact acatcataat ttggtgttaa
     3901 tacgtcaatt acttttccct taccttcatc gccccattgg aggcctaata acacatctac
     3961 ttttgccatt acaatttcta tatttggatt aaaacaaatt ggatttatat ttatttttcg
     4021 acccgtaaag atatgaaatt attacatgta acaagtcatg ctttttacta attatccaaa
     4081 aaaaatcttg caagccaaca aaatatatgt acctttgccg gacaataatt taaaaacata
     4141 gaacaatggc tcatgtaatt tcagaagact gcatcgcttg cggcacttgc atcagcgaat
     4201 gtcccgtgga agcaatttcc gaaggtgaca tctattcaat taatccagat atctgtacag
     4261 attgtggaac ttgcgcagaa gcttgtccaa ccggtgctat ccatccggct tgattttaaa
     4321 atgcacaaaa aaaacgagaa ctctcattga gaattctcgt ttttttattc tttcaaagcg
     4381 atagatctat tcagcatcat ctttagattc aatactatca ctattttgct ctttttcaaa
     4441 attattataa gaagtttgca tagccttacg cataaaattc agtgcctcgg tcattgcatc
     4501 ctcaaattta tcaaaatctt ctttgtacaa aaatattttg tgcttttcaa atacaaattg
     4561 accatcttct gtaacagcct tacgtttact ttctgtgatt gacaagaaca aatcatcacg
     4621 acgatctttt tttacatcta aatagtaaac acgctttcct gctttaaccg ttttagaaaa
     4681 tacaatctcc ttatcttctc tctcttcccg ttgaaccttt ggttcttctt tcggttgtct
     4741 gttaaacaaa ttaatcatca atcctatgtt taaatttttt cacacacaaa aatcattctt
     4801 ttttctcaaa ctgcaaaata aaaacacatt tattttgccc aagactattt tatcaattat
     4861 ttgttgtaac tttgcagcgc atattaatag gacatgtccg atttgtaaag ctatatagaa
     4921 gggataaatc ccaataaatg taatatcata acgatgatta atcgagtttt aatacgggta
     4981 aaaaccgttc agttattgta tgcgaactta aataatccga gttcgtcgca ggtttcggca
     5041 gaaaatgagc tacagactag tttggataaa acatacgagt tgtatcattg gttgttacaa
     5101 ttatcagtag atatcacaag ctacgctgtt aaacgtattg aaattggttt aaataaaatg
     5161 cgccctacac ctgaagagag taaccctaac aggcgtttta ttaacaacaa gtttgcaaag
     5221 cagctagcaa aaaacaagga acttgcaaaa tatgtaagtg agcatggtat atcttgggaa
     5281 gaaaacaatg atctggttaa aaacttgtat gaacttattt gtagaagtga tatatacaaa
     5341 gaatacatgg ctgcgccaag tagtgattac gagaacgaca aggtattttg gagacgtgtt
     5401 tacaaaaaaa tattgatgcc tgacaagtca cttgacgcac aaattgaaga aattaattta
     5461 tactggaacg acgactctga aaccgtgttt agtttcatag acaaaactat aaaacatttt
     5521 tgcgttgaaa atgaagaaaa tcagtcttta ttacccatgt atagggatga aagcgacaag
     5581 gaatttgcca tacaactata caaatttaca atagagaaca aagatgagta catgaaatgg
     5641 attgaagata cggcaaagaa ctgggaggtt gagcgaatcg ctgctatgga tatcgcaatc
     5701 atgcaagcag caattgcaga actaactcat ttcccaacaa ttccggttaa tgttacactt
     5761 aacgagtata tcgagattag taaattttat agtacagaaa aaagttgcac atttattaat
     5821 ggcgttttgg actcaataac aaacaagcta agaaaggaaa acaaactatt aaaagttgga
     5881 gcactagtaa aagatgattc taaataattt tttaaatata tcaatatgaa tttaactgtt
     5941 ttattacaag caactgccgc tacagaagga ggcatgggac aatatggatc aataattatg
     6001 ttggtcttga ttttcgttgt gttctatttt ttcatgattc gtccacaaat gaaaaaacaa
     6061 aaagaaatca aaaaatttcg tgatagcata tcagttggcg ataaagtaat taccgccgga
     6121 ggtatttatg gaaaaatcaa agaggtaaaa gacaatacta ttattttaga aatagccgat
     6181 aatgtccgca ttacagtaga caaaggttca gtatttgcaa ctgcagccga ttcggcaaca
     6241 gcagttgcta aataatgagt aaaaaagagc aaataagagt tttttctaac tttataaaaa
     6301 aggtaaatgc atttttgttt tctaaaaatg tatttatctt tttgtttttt ttaatcatat
     6361 catacgtgtt ttggtttatt atttcattga ataaaaacta cgaaacacgt cttacttttc
     6421 ccctggagta taggaacatt cctaaggaaa tagctttatc aacagattta ccatcatcta
     6481 tagatgtaaa ggtgaaagat aaaggaactg taatcttcac atataaacaa agcaagtttt
     6541 ctgcattagt aattgatttt aaagatattc cgggattttc aaaaaataaa agcgcagttt
     6601 tatcgacatc agataccttt gacaaaaaaa tcaaagaaca acttgctcaa acaacagtaa
     6661 tcacagatta ttttccttca gaaataacaa tagaacgaat ctttttatcg tcaaagaaac
     6721 tgccgatacg tataaattgt gacatacgat ttgcaaagca atataactta agcgatagta
     6781 tcaaagcaaa tcctgattct atcattctat atggcacaga agaatgctta gacacaaccg
     6841 tatatatcag cacaaattta ctgcgcataa aagatattaa agacacatta aataggtctg
     6901 tgggtttatt attaccaaaa cataccaaag ctgataattc aaaagtcaat ttaatgattc
     6961 cggtagaagt ttctactgaa gcttctataa tgattccggt aaaagttata aacacaccca
     7021 aaaatatacg cgtacgagtg ctccctggtg aagttaaatt gacatatatg gtcggtataa
     7081 gcaaatataa gaaagtaaag tcatctgatt ttacagtttc tattgactac gaagatttaa
     7141 ttgaaagcag ttcgaaaaca gaattcgtta atattgacac taaaccaagt tttgtatcaa
     7201 attgccgaat taatccacaa gaagttcaat gggttatgga agttattgaa gattaaacta
     7261 tatgataatt ggagtaacag gaggaattgg cagcggtaaa actcactttt gccatttaat
     7321 ggaaatcgca ggttttcctg ttttctattc tgacacagaa agcaaaaaac ttcttaattc
     7381 agatccttcg atcaaagcac agataataga tttgtttgga gcatcagcct actcagaagg
     7441 tgttttagac tcaaaacaca tcgcacaaca agtgttttct aataaaaaat tacgcactaa
     7501 attaaataat atagtacatc ctgccgtaaa gaaaatattc gacttatggc ttatcgaaca
     7561 cgaatctagt cctataatat tattggaatc tgcgatacta ttcgaaagtg gttttgatgc
     7621 ctttgtggac aaaataatag tgatcgcagc acctattgaa actagaataa aacgagttgt
     7681 tcagagagat aaatgcgaag aaaaagatgt tttatcaaga atgaaatcgc aaatgtccga
     7741 cgagatcaaa ataagcaaag ccaacttcgt tattcaaaac ggagaacatg atgacttaaa
     7801 caaacaaatt tttgatttac ttggaaaact aatgtcccca aaataaaaat tggccgacaa
     7861 attaatagac cctacatttt aaatttataa cggcaattaa acttaccttt tgtcattgca
     7921 actaatttcg aagacagcat ctgtcgtctt atcaaagaca ctttatcaac aaacacatga
     7981 ccatcaatat gatcatactc atgttgaata gctcttgctg caaatcctga aaaagtttca
     8041 gtcttttcat tccaattttc atcgtaatag ttaatagtta tactattcgg acgacgaact
     8101 gattctgata taccgggaag acttaagcaa ccttcttctt ttaaagaatc atcctcgcta
     8161 taagatataa tttgggcatt aataaaggtt ttcttgaatg tagctaaact aggaatttca
     8221 tcagccatta aatgtaaatc tacaacaaac aagcgtagcg acaaacctat ctgtggagct
     8281 gctaaaccaa ctccatctgc tttttcaaga gtttgaaaca tgtcagaaat caactgttca
     8341 agattagaat aatcagcagg aacaggaagt gctactttct taagtacggg atttccgtaa
     8401 attacaatcg gatatatcat attttcattc tttggctttc catataatct tgcaatataa
     8461 tggtcgcact aatttcatct actaaagctt tgttttgtct atctttcttt ttcaaaccac
     8521 cggttaacat tgttttgtgt gccaaaacag atgtaaaacg ctcgtcgaaa aactcaacgc
     8581 gaacattagg aaatttattt ttaagagaat ttacaaatgg tgtaatatat ttcatcgact
     8641 ccgatggcat attattcatt tgcttgggca atccaaccac tatacactct acactttcgg
     8701 ttgcaagata tttcgctaag aaagttaata tctgctgcga atcaacggtc gttaaaccac
     8761 ctgctattaa ttgagaagga tcagacaccg ctattccgac tctctttttt ccgtagtcaa
     8821 ttgctaaaat tctagacatt atcgtgcaaa gataggatat tttatgtaca tttgcgatat
     8881 taatacgtac aaatgtcaat atgaaaattc tagttacagg tggaaccggt tatatcggtt
     8941 cgcatacagt cgttgaacta caaaacgaag gttttgaacc aataatcata gataatttat
     9001 ctaattcatc aatttcagta ctagaaggaa ttgaaaaaat tacaggtaaa agacctgtct
     9061 ttgaacaaat agactgcaaa gatattaatg acatggattt attgtttact aaacataaag
     9121 atatcgttgc aatcatacat tttgctgcca gtaaagctgt tggtgaatct gttataaagc
     9181 ccctaatgta ctaccgaaac aatcttaaat ccatgctaaa cataatggaa ttaatgatta
     9241 agcacaatgt tgagaatctt gtattttcat catcatgtac aatatatggt caacctgaca
     9301 ttcttcctgt aacagaaaaa gcagaaatca aacctgcact ttctccatat ggaaatacaa
     9361 aacaaatatg cgaggaaatt attaatgata ctgcacacgc aaatcctcat attaacgcta
     9421 tactgctaag atattttaat cctataggag ctcatgaaag ttctgaaata ggcgaacttc
     9481 caataggtgt gcctcaaaat ttaattccat acgttacaca aactgcagca ggcatacgta
     9541 aacaattaca agtttttgga aacgattata acacacctga tggttcttgt atccgcgact
     9601 acataaatgt tacagatttg gcaaaagccc atgtagcttc tgttaaaaga ttgcttgata
     9661 aaaaagggaa aagttctgtt gaagcattca acataggaac cggcagaggg actacggtat
     9721 tagaagttat atctttattt gaaaaagtta caggcgtgaa gctaaactac aagatagttg
     9781 gtcgtagaga aggtgatatt gaaaaagtgt gggctgatgc atcactcgca aataatgaat
     9841 taggatggcg tgcagagaaa agtatggaag acacattgct ttctgcatgg aaatgggaac
     9901 aacacttaaa acaaactaaa acgagatgaa aaagagacta tttctaatgg gaatttgcat
     9961 ttttgctaca agcttggtag gttcagcgaa aaaagcagtt gtggtaagta ctttacaatt
    10021 accaccggcc gaagcaattt acaacgaagg tatcaatttg ctgcgtcaaa aacaatatga
    10081 tgatgccata attaaattca ctgctacaat tgcagaggat actacattta caaaagctta
    10141 ttacaacaga gcaatcgcaa attttgaaag tgaacgttac aataaagcca tgaatgattt
    10201 aaatgtagct ataagacaag ccaataacga aactgcttct gaatgttatc tactaagagg
    10261 taaaatcatg tttcatctgg atgatattaa aaacgcatta accgatttag ataaagctat
    10321 agaattaaat ccgtctaata ttaatgcaag attagataag gctgccttac ttcaatataa
    10381 gcgcgaatac gcattggcag aagaagagta ctccaacata ttaaatatca gtccggacaa
    10441 tgcttctgca ctactagaaa gaggaaattg ccgtcaatac atcgggcaac ttgaagccgc
    10501 aatgaatgat tacaaaattt tagcaataaa tgattcatca aattttcaag ccaaacttaa
    10561 tattgcaact ttaacttgga aaatttcagc agatacggta aatagcatat cacttctcaa
    10621 taaattagca aaagacaacc ctcaaagtgc tgaagtgttt aacgcacgcg gactcatata
    10681 tagtttaact gcacaatacg attctgcaat tgcggatttt gacaaagcaa tagcgataaa
    10741 taaaaattat gcagctgcat acaataacag aggctcggct tattataaac aaaataaatt
    10801 agaaacagca atagacaatt acaaccgtgc aatagaaata aacactaact atggtgatgc
    10861 gtatctgaat cgtgctatag ttagggaatt actacgagac gagaatggtt cgtgtaatga
    10921 tttaaaagca gccgccaaac taggagttaa aaatgcatct gaatatttta ctaaacaatg
    10981 tgaataactt agccatgaaa aaaatatcta tcatactaat ttgcactttg gcatgtaatt
    11041 tcttattcgc tgccaaaact aatattccgc ttagcaaaga ttcgctaaaa tcatacaaaa
    11101 cagcggtaaa acaaggtgat aaattttatc gtcttcaaga tgagaaaaaa tatcaggcct
    11161 gcggtcaata cttaactgct tacaatcttt atgcaggaga tgcttcttta tgctacaaat
    11221 taggtaattg ttatttgact ggtttttcta aaacaaaagc tattactttc tttgatgcag
    11281 ttgctgacat agattcgaca cacaaacaat ttcctgatat atattggaaa cttggaactg
    11341 catatcaatt agacaatcaa tttgacaatg ctatagtttc atatcagaat cacaaagaca
    11401 ttctaagtaa gggcaaacag aacaaaaaaa caattgctaa aattaaacaa acagataaac
    11461 gtatacaaga atgtcagtcc ggattaagtt tagaaaaaat taaaaacccc ctatttattg
    11521 acaatgtttc ggcaatcaac acttctgcag atgaatacaa tactatttgc aacccaaatg
    11581 gtaaaacaat aagttacact aatgcaacat tattatcttc aggagagcgt cgtgagcact
    11641 ct
//
LOCUS       DAOPID010000022        11638 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_6630_length_11638_cov_4.795476, whole genome shotgun sequence.
ACCESSION   DAOPID010000022 DAOPID010000000
VERSION     DAOPID010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 11638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11638
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6630_length_11638_cov_4.795476"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4978)
                     /locus_tag="PK641_01680"
     CDS             complement(<1..4978)
                     /locus_tag="PK641_01680"
                     /inference="COORDINATES: protein motif:HMM:NF015497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HPG55099.1"
                     /translation="MKKKRNTTLLLLAAFILLPSGEISGSEQKNIVLQEETTNPWANY
                     TAEESIRLQPGFHYQAKSGQLWGKSTFVGKIDQGLLFPPTEGEYAASDGSFAASPSDG
                     AIVGSIGGEMSVSPSGSAVYTIPIECPDGINGLKPNVSLIYNSGSGDGIAGWGWQIGG
                     LSSVIRVGKTPYIDDERTGIEFTVSDALSLDGQRLIKITDYGDSAEYKTEAENFSRIM
                     GYEKHSWGFKYFIVYTKEGHRIRYGSNQENASEASYFPLAKADFKNTGIHIFRHLAWL
                     LEEIVDANGNAVQFFYTVSEQSTDNVILSVKNYQDCRLDRIVYGTNTVIGIQGEQVIQ
                     FIYRDKNSSTKYVSGEPVVISKLLDRIEVKSNGQNYRTYQLTYDIESEKYRLRSLTLA
                     AENEHVNTMLFEWNNMQYGCHFNGVKQYPLSSMMQTYFNDGYTNVYRNSIHIGDIDND
                     GLTDVFISYRMQKKSMTSFADPATVNIGVVYRNTGTSYEIKKDTIVPLSDAYFSDKDN
                     DGRDELYVGSAGDKSYTSVCYKFDSNGNFVVKNTESYSFPDLTGFVYPIIGDFRGNGT
                     AQTIFISRHRGNVKVAYSPEDNFDLSQIQSYASVPVIYAADMNGNGKSDLICVCRDRV
                     SVYELNGRTFMPIFQDAQFGFTYTDKLEFGDFNGDGNADIIASGSSGIKFYLSTGLKY
                     QEVNMPSGLNSNSNFVLTDADKDGLTDIVTWYENNNGYNIQLLINKANCFEVVSSGTY
                     SEPFSVLYEGMINRRDRSDICLLVRNAALSEPSISFTRGNVSHVLSSVTDGFGNKTVI
                     QYTPFHSSGFVSTSFKSAINESDIQSYSKLDRGHWHVVESIKMYDSQNETASKQSYAF
                     SNAYVHRQGKGFLGYERMSCTDSLQGIITTKTNQLHLGHYFLIPTNETIKTIGGTPIR
                     HTDNAFTVTSLTDNRYMMLPTRIMENDDLTGLYSVTTYANYANGNPGKVIIRQGDCSE
                     IKEIGYGIAGSWCPNKPVYMSVKKQQGNSFSPTHYNYYSYDTRGNLTYESNDAGDSNQ
                     LITHYQNINAFGMPETITVTANGKTRVTRCTYTPSGRYITSKTNILGETTTYVWNEAK
                     GLLLSQTDAYGRTTTHTYTGWGNLKETKYPDGIRYTEKLLWAERGNDMGAAYYEYKET
                     SGQSPKYIYYDSMGKEICSQSYGRNGRLVSVFTEYNTAGQKYRVSEPTFSSVADTWAA
                     TYSYDLFGRPIRVVTPMGNTTTSYTGMSITVNSPDGVTTTLKNTSGQTETVTTNGQSV
                     SYTYHPDGNVASATPAGGEPITMEYDLQGNRIRLTDPDAGVVESYYNGFGDLLWERQK
                     VHTDTWITTSHQYADNGELQQIVRGDETTSYLYDDHHRVSSIEIAGKHKQSFCYDDKD
                     QIVELKEEFDGKTFKQKTEYDFFGNVKKEIYPSGYYILKSYDAYGFLTQVSDSRNRDI
                     LRIEEENASGQIVRKIRAGRETSYTYDSRHLPIRIFSSDIMDMSYVFDVNGNLQSRTD
                     ALSNQREAFSYDALQRLTGWNTYRNDSLRRSASLQFDASGNIVSKSDLGGFAMRYGDN
                     GRPHALTSIDSLPPDFPHGNLHLQYTDFKKLKLMYNETGDSYVIDYGVDDQRRKSILQ
                     SSNEIVETRYYFGNYEETCDVHGQVKKIHYLSGGAMLVIDEAGETMYYG"
     gene            complement(4982..5437)
                     /locus_tag="PK641_01685"
     CDS             complement(4982..5437)
                     /locus_tag="PK641_01685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPG55100.1"
                     /translation="MKKTIILTIVFLLNVVFLYAQCSVEYSYDYAGNRTERQVICLGT
                     TRSAEEFIEEDLDVDDRNIQPVMSLMDNLNIRITPNPTRGLIGVEIEGACETDVLMLT
                     VCSIGGKLLQTEVLKLGINPIDLSGYANGHYILQINSGTSRKEFKLIKY"
     gene            complement(5446..6618)
                     /locus_tag="PK641_01690"
     CDS             complement(5446..6618)
                     /locus_tag="PK641_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55101.1"
                     /translation="MKKHFLREFLLAVGMVVASQTTITSQTAQLLRVEGDGDKFYPVA
                     FDDSKWDSSMRTILEINRFSTHLDSSWKGALCARFEYHLTRGGNGANFINADVHQSVV
                     NVNPAEFVAGWQDISGNSGQAKMLVWLKGNTSYYYVSNGAQSPLVYDGVQNPLPLTFG
                     EWGEPVSYTYKTTIDESVVDGASMVYNSLRIGTIGDPGNLNVPLNAQAAQYNIDFTGY
                     RDCTPDQVGARIAAKRINRHQENAALIQKTGLAFYTNPDGWNGGDVDLQERMCISPDG
                     KVGIGTSLPDHLLTVNGIIHAKEILIELSGPLADYVFDSTYVLMPLKEVNEYVKSHKH
                     LPDMPSAADAVSSGISVGDMQNKLLQKVEELTLHAIRQQEEIDALKNELRNMTQTR"
     gene            7121..8917
                     /gene="typA"
                     /locus_tag="PK641_01695"
     CDS             7121..8917
                     /gene="typA"
                     /locus_tag="PK641_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HPG55102.1"
                     /translation="MQNIRNIAIIAHVDHGKTTLVDKMLVAGALFRDNEKAGELILDN
                     NDLERERGITIVSKNVSINYKGTKINIIDTPGHSDFGGEVERVLNMADGVLLLVDAFE
                     GPMPQTRFVLQKAIEIGLKPIVVINKVDKPNCRPDEVQEAVFDLMCSLDATEDQLDFP
                     TIYGSAKQNWMSKDWRNKTDNIYALLDAIIQYIPAPQTLEGPLQMLITSLDYSSYVGR
                     IAVGRVHRGVIKENKDIMLIHRDGTSVKCRIKELNVFSGFGKTKVSEVASGELCAIVG
                     LDKFEIGDSICDIENPEPLPPIAIDEPTMSMVFTINNSPFFGRDGKFVTSRHVNDRLI
                     KELDKNLALRVVKEDKTDIWHVFGRGVLHLSVLIETMRREGYELQVGQPQVIIKEIDG
                     QKCEPIEQLTVNVPEEFSGKVIEMVSNRKGEMLVMETKNDRIHLEFEIPSRGIIGLRT
                     NLLTATTGEAVIAHRFIKYAPYKGDIERRTNGSIIAMESGTAFAYAIDKLQDRGKFFI
                     YPQEEVYAGQVVGEHCKDGDLVVNVTKSKKLTNMRASGSDEKAKIVPPIVLSLEEALE
                     YIKEDEYVEVTPHAMRLRKIILDELERKRSNK"
     gene            8892..9392
                     /gene="folK"
                     /locus_tag="PK641_01700"
     CDS             8892..9392
                     /gene="folK"
                     /locus_tag="PK641_01700"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HPG55103.1"
                     /translation="MNEREATNKVYIALGGNLGNKVSVFCMAEELLSKEIGDIISKSS
                     NYESEAVGFYSNNTFLNNVICLITQLSPLQVLSITQDIEKRLGRTQKSVQSNYKDRII
                     DIDILYYNNIIINTYELTIPHPHLHEREFVLKPLLEIAPKKIHPAFLLNTEEMWFRYQ
                     NQHLQV"
     gene            complement(9389..10375)
                     /locus_tag="PK641_01705"
     CDS             complement(9389..10375)
                     /locus_tag="PK641_01705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HPG55104.1"
                     /translation="MKAKFNTLVVLLILPLFNLAAQDFLLSQPWSATTTIAPSFAGFT
                     GGGRVYTTYRNQWSAIEGSQTILADYDQYFHPIRSSFGGLLSYTSYAGGMLSQLQFDI
                     QYNYAVQMTKHWYFRPGLQLSVFYRTIDPSKFVFVDQIAMDGTILPTSSFQTSINSLF
                     RFDAAVSVMFYNEYFFGGLSVSRLLGNNVSFTDAPSTTDPLKLNVYAAGMIPISKGYG
                     KYDPKDDVTIATLYQWQGQYHQIDIDVMYHRRYFMAGIGYRGIPFYSPDGLTNTDALK
                     VMVGGSVAGFSVSYSYDVSLSSLVNVSGGSHEVVLTYRFMEKAPNDRSFFCY"
     gene            complement(10375..>11638)
                     /locus_tag="PK641_01710"
     CDS             complement(10375..>11638)
                     /locus_tag="PK641_01710"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPG55105.1"
                     /translation="TICDDSVTLRAPKPLYGDYRWTLISGDGVLQDPTSNVSLITDLS
                     NKVTNIIRWEVYTESCSNTIDVEVVSHSLTHLVDAGDDGATINGSYRLSARSVADTNV
                     TGRWSIVAGSGVFDDETAPNTYVTGLAPGINTVRWTLSAYDCEAYDELQVRAVDEPIA
                     DFSADPIVGCKPLAVIFTNTTLGDAEYLWDFGDGTTSTKRSPDHVYTQSGTYTVTLTA
                     TGTRKSDQVSKDITVYVSPVAEFSVSLTQLYTPNAEAHFYCKSDKVISYLWDFGDGGQ
                     STEKDPTYIYREDGLFDVSLVVENSFGCKDTLKLDEYVRVGKGSFITFPTAFVPNLTE
                     ANGGVYSPEERRLDIFYPVSRNVDTYKLEIFNQWGVKVFVSEDLYQGWDGYYLGKCAQ
                     QATYSYKAEGYFKDGTSFRQSGNFMLVR"
BASE COUNT         3666 a         2399 c         1970 g         3603 t
ORIGIN      
        1 acccatagta catcgtttcg cccgcctcat caatcaccaa cattgcacca ccgctaaggt
       61 agtggatttt ttttacttgc ccatgaacat cgcacgtttc ttcgtaatta ccaaaataat
      121 accgcgtttc aactatttcg tttgaacttt gaagaatgct tttgcgtcgc tgatcatcca
      181 caccgtaatc aataacgtaa ctatcacccg tttcattgta catcaacttc aattttttga
      241 aatcggtata ttgaagatgc aaattcccat gcggaaaatc gggtggcaaa ctgtcaatcg
      301 aagtaagggc atgtggacgt ccgttatcac cataacgcat tgcaaatccg cctaaatcgg
      361 atttacttac aatattacct gaggcatcaa attgcaaaga agcagatcgt cgtaacgaat
      421 cgtttcgata agtattccat cccgtaagac gttgtagcgc atcataagaa aatgcttctc
      481 tctggttgga taatgcatca gtacgagact gcaaattgcc gttaacatca aacacatacg
      541 acatgtccat tatatcgctt gaaaaaatac gaatcggcaa atgacgacta tcataagtat
      601 atgaggtttc tctgcctgca cgtatctttc gaactatttg accacttgca ttttcttctt
      661 ctatgcgcaa aatatctcta ttacgagaat ctgacacttg agttaaaaag ccgtaagcat
      721 catagctttt taagatataa tacccgctcg gatatatttc cttttttaca ttcccgaaaa
      781 agtcatattc cgttttttgt ttaaaggttt ttccgtcaaa ctcttctttt aattcaacaa
      841 tttgatcttt atcgtcgtag cagaatgatt gtttgtgttt ccctgctatt tctatagagc
      901 tcaccctatg atgatcatcg tacaaataag aagtagtttc atcacctcgg acaatttgtt
      961 gcaattctcc attatcggca tactgatgac tcgttgttat ccacgtgtct gtatgcacct
     1021 tttgcctttc ccaaagcaaa tcaccgaagc cattgtaata cgattctaca acaccggcat
     1081 ctggatctgt aagacggatc cgattccctt gtaaatcata ctccatggtt atcggttctc
     1141 cgcctgcagg cgtagcagat gcaacgtttc catccggatg atacgtataa gaaacacttt
     1201 gtccattggt tgtaaccgtt tcggtctgtc cagaggtatt ctttaacgta gtagttacgc
     1261 catccggcga attgaccgta atagacatcc ccgtatacga agtggtagta ttgcccatcg
     1321 gagtaactac tcgaataggg cgaccaaata aatcgtaaga ataagttgca gcccatgtat
     1381 ctgccactga agaaaacgta ggttctgaaa ctctgtattt ttgcccggct gtattatatt
     1441 cggtaaatac cgatacaagc cttccgtttc gtccgtacga ctgtgaacat atttctttgc
     1501 ccatcgaatc gtaataaatg tatttcggcg attgtccaga tgtttcctta tattcatagt
     1561 aagctgctcc catgtcatta cctctttctg cccacaacaa tttttcagta taacgaatgc
     1621 catcgggata ttttgtttct ttcaaatttc cccaccctgt atatgtatgc gtagtggtgc
     1681 gtccataagc atccgtctgc gaaagaagca atcctttggc ttcgttccaa acgtacgtgg
     1741 tagtttcgcc aagtatattg gtcttagatg ttatatatcg acccgatggc gtgtatgtac
     1801 atctagtaac acgtgttttc ccattggccg taaccgttat ggtttccggc attccaaagg
     1861 cattgatatt ctgatagtga gtaattaatt gattagaatc gccggcatcg ttcgattcat
     1921 atgtcaagtt accacgggta tcataactat agtaattata atgagtgggc gaaaaagaat
     1981 ttccttgttg tttctttacc gacatataaa caggtttgtt cgggcaccat gaaccggcaa
     2041 ttccatagcc gatttctttg atttcggaac aatcgccctg ccttattata actttacccg
     2101 gattaccgtt tgcataattg gcataagttg ttacgctgta taatcccgtt aaatcatcgt
     2161 tttccataat tcgcgttggc aacatcatgt atcggttatc ggttaacgat gtaacagtga
     2221 acgcattgtc tgtatgtcga atcggggttc caccaatcgt ttttatcgtt tcatttgtcg
     2281 gaattaaaaa ataatgtccc aaatgcagtt gattggtctt cgtagttatt ataccttgta
     2341 atgaatccgt gcatgacata cgttcgtatc ccaaaaagcc cttcccttga cgatgtacgt
     2401 acgcattgga aaacgcatag gattgtttag aagccgtttc gttttgtgag tcgtacattt
     2461 taatactctc aacaacatgc caatgacctc tgtccaactt cgaataggat tgaatatccg
     2521 attcattaat tgccgatttg aatgaggttg atacaaaccc actggaatga aacggcgtat
     2581 actggattac cgttttattg ccaaaaccat ctgttactga tgaaagtaca tgcgatacat
     2641 tccctctcgt aaaagaaata gaaggttcag ataaagcagc attacgaacc aacaaacaaa
     2701 tatcagacct atctctacga ttaatcattc cctcatatag tacagaaaac ggttctgaat
     2761 acgtacctga cgaaaccact tcaaaacaat tagctttatt gatcaagagt tgaatattgt
     2821 acccattgtt attctcatac cacgttacaa tatctgtcaa tccgtcttta tcggcatctg
     2881 tcaatacaaa attagaatta gaattaagtc cgcttggcat attcacttct tgatatttca
     2941 atccagtaga taaataaaat tttatacctg aagaaccgct ggcaattata tccgcatttc
     3001 catctccgtt aaaatcacca aactccaact tatcagtata tgtaaaaccg aattgcgcat
     3061 cctgaaaaat aggcataaat gttctgccgt ttaattcata aactgaaacc ctatcacgac
     3121 aaacacatat caaatccgat ttgccattgc cattcatatc agcagcataa atcacaggta
     3181 cactagcata agattgtatc tgtgataagt caaaattatc ttccggcgaa tatgctacct
     3241 ttacattgcc tctatgacga cttataaaaa tagtttgagc ggttccattt cccctaaagt
     3301 cgccgattat gggataaaca aaacctgtca gatccggaaa tgaataagat tcggtattct
     3361 taactacaaa attgccgttg ctatcaaatt tatagcaaac actcgtatac gatttatcac
     3421 cggcacttcc aacatataat tcatctcgac catcgttgtc tttatctgag aaataagcat
     3481 cggataatgg tactattgta tccttcttaa tttcataaga agttcctgta tttcgataaa
     3541 caactccaat attaaccgtt gcaggatctg caaaagaagt cattgatttt ttttgcatcc
     3601 gatatgatat aaatacatct gtcagcccat cgttatcaat atcgccaatg tgaatagaat
     3661 ttctataaac attagtatat ccgtcattaa aatatgtctg catcatagaa gaaagcggat
     3721 attgtttaac gccattaaaa tgacatccat attgcatgtt gttccactca aacagcatgg
     3781 tatttacgtg ttcattttcg gcagcaagtg tcaatgacct taaacgatat ttttccgact
     3841 ctatgtcgta tgtcagctgg tatgtacgat agttttgtcc attgcttttc acttcaatac
     3901 gatccaataa tttagaaata actacaggtt cacctgacac atattttgta cttgaatttt
     3961 tatctcggta aataaattga ataacttgtt ctccttgaat accgatcact gtatttgttc
     4021 catatacaat acgatccaaa cgacaatctt gataattctt cacgctcaaa attacattat
     4081 cagtagattg ttctgaaaca gtgtaaaaaa attgaactgc attgccattg gcatcaacaa
     4141 tttcttccaa taaccaagcc aaatgcctaa aaatatgaat gccagtattt ttaaaatccg
     4201 cctttgccag aggaaaatag gaggcttctg acgcattttc ttgattgctt ccgtagcgta
     4261 ttcgatgtcc ttcttttgta taaacaataa aatacttgaa gccccatgaa tgtttttcat
     4321 accccataat acggctgaaa ttttccgctt ccgttttata ttcagcgcta tcaccataat
     4381 cggttatttt aattaaacgt tgtccatcaa gcgacagagc atctgaaacg gtaaattcaa
     4441 tgccggtacg ctcatcgtca atatacggag ttttccctac tcgtataaca gaagatagtc
     4501 cgcctatttg ccaaccccat ccggcaattc catcaccgga tccactattg tagattaacg
     4561 aaacattcgg tttaaggccg ttaatgccat ccgggcattc aatgggtatc gtgtacactg
     4621 ccgatccaga aggactgacc gacatttctc ctccaataga accaacaata gctccatcac
     4681 tcggtgaagc tgcaaacgac ccatcagaag cagcatattc tccttccgta ggcggaaata
     4741 gcaagccttg gtctattttc cccacaaacg tagattttcc ccacagctga ccagatttcg
     4801 cttgataatg gaaacctggt tgtaatcgga tactttcttc tgccgtataa ttagcccatg
     4861 gattagttgt ttcttcctgt aaaacgatat ttttttgttc acttccagaa atttcaccgg
     4921 acggtaataa tatgaaggct gccaacaaga gcagtgttgt atttcgcttt tttttcatga
     4981 atcagtactt aatgagttta aactctttac gtgaggttcc gctatttatt tgcaagatat
     5041 aatgcccatt agcatagcca gataaatcta tcggatttat ccctaatttt agcacttctg
     5101 tttgcaagag ttttcctcct atagaacaaa cggttagcat aagaacgtca gtttcgcacg
     5161 ctccttcaat ttcaacacca atcagtccgc gagttggatt cggagtgatt ctgatattga
     5221 gattatccat taggctcata acaggctgta tatttctatc atcgacatcc aaatcttctt
     5281 caataaattc ttctgccgaa cgagtagtac ccaaacaaat cacttgacgt tctgtacgat
     5341 tacctgcgta atcgtaacta tactcaactg agcattgagc gtaaaggaag accacgttca
     5401 acaaaaaaac gatggtcagt attattgttt ttttcatttt aatgattatc tggtttgcgt
     5461 catatttctt agttcatttt tcaacgcatc tatttcttct tgttgtcgaa tagcatgcaa
     5521 tgtcaattct tctacctttt gtaacaattt attttgcata tctccaacac ttatacccga
     5581 agaaactgca tctgcagctg aaggcatatc aggcaaatgc ttgtgacttt tcacgtattc
     5641 gttaacctct ttgagcggca tcaaaacata cgttgaatca aatacataat cagccaatgg
     5701 tccggacaat tcaataagaa tctctttagc atgtataata ccgttcactg tcagcaaatg
     5761 atccggtagt gacgttccta tgccgacttt accatcggga gaaatacaca ttcgttcttg
     5821 taaatccaca tcaccaccat tccatccatc tggatttgtg taaaaagcaa gtcctgtttt
     5881 ttgtatcaaa gcagcatttt cttgatgtcg attaattcgt tttgcagcta ttcttgcccc
     5941 aacctgatca ggagtacaat cgcggtatcc agtaaaatca atattatact gtgctgcttg
     6001 tgcatttaac ggaacattca aattaccagg atcacctatc gtgccgattc gcaggctatt
     6061 gtataccata gaagcaccat ctaccacaga ctcatcaatc gttgttttgt atgtatacga
     6121 aactggttct ccccattccc caaacgttaa tggaagagga ttttgcacac catcgtatac
     6181 aagcggcgac tgcgccccgt tcgatacgta ataatatgaa gtatttcctt tgagccatac
     6241 caacattttt gcttgaccgc tgttgcccga aatatcctgc cagcccgcta caaattcagc
     6301 cggattaaca tttacgacag attgatgcac atctgcatta ataaaattgg ctccattacc
     6361 tcctcgcgtt aagtgatatt caaagcgagc acacaaagct cctttccatg aagaatccag
     6421 atgcgtacta aaccgattta tttccaaaat ggtgcgcata gatgaatccc attttgaatc
     6481 atcgaaagca accggataaa atttatcccc atctccttca actcgtaata attgtgctgt
     6541 ttgtgatgta attgttgttt gtgaagcaac aaccatacct acggcgagca gaaactcgcg
     6601 caaaaaatgc tttttcattt gcttaccctc gttttttgtt attttttaat tatttattat
     6661 tttagttatt ttttacaaaa acgagacaaa atataaaact attttactaa aatgcaagca
     6721 tatttgcctg aataatataa acaaaaactc atcatttttc atatcacact ctgtttcaaa
     6781 taaatcatat ataataatga ctcattagcg gaattaaatt agtgcgtatt aatcttaggt
     6841 tttgctatta atgaagttac taaattatag gattgtacgt gcactaagct tattgataat
     6901 cctaataaat aatcaatctg tttttttgca taatttcttc atataagaaa ttagtcacac
     6961 attttagata ataacgtttc tattattcgt gctttgacaa aaagaattaa taatttcact
     7021 taaacattac gtattttagt ttaaaatgat gatttattga acataaacta tttattttca
     7081 attaaaatgc gtaccttcgc agccgcaaaa aaacgttttt atgcaaaaca ttagaaatat
     7141 cgccatcatt gcccacgtcg atcacggaaa aacgacatta gtagataaaa tgctagttgc
     7201 cggcgcgtta ttcagagaca atgaaaaagc tggcgaactt attcttgaca acaacgatct
     7261 ggaacgtgaa cgtggtataa ccattgtgtc aaagaatgta tctatcaact acaaaggaac
     7321 taaaatcaat atcattgata ctccgggaca ctctgatttt ggtggagaag tagagcgtgt
     7381 tcttaatatg gccgatggtg tactgctatt agttgatgct tttgaaggtc ctatgccgca
     7441 gacgcgcttt gtactacaaa aagcaattga aatcggatta aaacctatcg ttgtcatcaa
     7501 caaggttgac aaacctaatt gccgtcctga tgaagttcaa gaagctgtat ttgatttaat
     7561 gtgcagtttg gatgctacag aagatcaact tgatttccct actatttacg gatcagccaa
     7621 acaaaactgg atgtcaaaag attggagaaa caaaacagac aatatatatg ctcttcttga
     7681 cgctattata caatacatac cggctcccca aacattggaa ggaccacttc aaatgctaat
     7741 aacatcattg gattattcat cttatgttgg acgtatagca gttggccgtg tacaccgagg
     7801 agttataaag gaaaacaagg acattatgtt gatccatcgc gatggaacat ctgtaaaatg
     7861 taggatcaaa gaacttaatg tattttcagg atttggaaag acaaaagtat ctgaagttgc
     7921 atcaggtgaa ttatgtgcaa ttgttggact tgataagttt gagattggcg atagtatttg
     7981 tgatatagaa aatccggaac cacttcctcc aatagcaatc gacgagccaa ctatgagtat
     8041 ggtatttacc attaacaact cgccattttt tggtcgcgat ggaaaattcg ttacatcaag
     8101 acatgtcaat gatcgtttaa taaaagaatt agacaagaat cttgctcttc gtgttgttaa
     8161 agaggataaa accgatattt ggcacgtgtt tggtcgtggt gtattacact tatccgtatt
     8221 aatcgaaacg atgcgtcgtg aaggttacga gttacaagtt ggtcaaccac aggtaataat
     8281 taaagaaatt gatggtcaaa aatgcgaacc aattgagcaa cttacagtta atgtacctga
     8341 agaattttca ggcaaagtaa tcgaaatggt atcaaatcgt aaaggtgaaa tgcttgtcat
     8401 ggagacaaaa aacgaccgta ttcatttaga gtttgaaatt ccatctagag gtatcatagg
     8461 tttacgtaca aatttactta cggcaacaac aggtgaagct gttatcgctc atcgttttat
     8521 aaaatatgct ccatacaaag gtgatattga acgccgcaca aacggatcaa taattgcaat
     8581 ggaaagtggt actgcttttg catacgctat agataaatta caagacagag gtaaattctt
     8641 tatatatcca caagaagaag tttacgcagg acaggttgtt ggagaacatt gcaaagatgg
     8701 tgatttagtt gtaaatgtta caaaatcgaa gaaattaacc aacatgcgtg catcaggttc
     8761 tgacgaaaag gccaaaatag taccaccgat agtattaagc ttggaagaag cgttggaata
     8821 tataaaagaa gacgaatatg ttgaagtaac tccgcacgcc atgaggttgc gaaaaataat
     8881 tttggacgaa cttgaacgaa agagaagcaa caaataaagt atacattgca ttaggcggga
     8941 acttaggtaa taaagtttcc gttttttgta tggcagagga actactgtct aaagaaattg
     9001 gtgatattat ttcaaaatca tcaaactacg aatctgaggc agtaggattt tactccaata
     9061 atactttttt aaataatgtc atttgtctaa ttacacaact ttctccatta caagtattat
     9121 ctattacaca agatattgaa aagcgattag gacgcactca aaaaagcgta caaagtaatt
     9181 acaaagatag aataatagat attgatattt tgtattataa caacataatt attaatacat
     9241 acgagctgac aataccacac ccgcacctgc acgaaagaga gtttgtatta aaaccgcttt
     9301 tagaaattgc cccgaagaaa atacatccgg catttttact aaacacagaa gaaatgtggt
     9361 ttcgctatca gaatcaacat ctgcaagttt aatagcagaa gaaagaacga tcgttaggtg
     9421 ctttttccat aaaacgatac gttagaacta cttcgtgaga accacctgaa acatttacca
     9481 aacttgacag actcacatcg tagctatatg aaacactaaa gccggctacc gaacctccta
     9541 ccataacttt taaggcatca gtgttggtaa gtccatcagg cgaataaaat ggaatacctc
     9601 tatagcctat tcctgccata aaatagcgac gatgatacat tacatcaata tcaatttgat
     9661 gatattgacc ttgccattga tataatgttg ctatagttac atcatcctta ggatcatact
     9721 tgccgtagcc ttttgaaata ggtatcatac ccgctgcata aacatttaat ttaagtggat
     9781 ccgtagtaga tggggcatct gtaaacgata cattattccc caacaaacgg cttacactta
     9841 aacctccaaa aaaatattca ttatagaaca tcacagaaac cgcagcatca aaacgaaata
     9901 gagagttaat actcgtttga aaagaacttg taggcagtat tgttccatcc attgctattt
     9961 gatcaacaaa cacaaatttt gatggatcaa ttgttctgta aaatacactt agctgtaagc
    10021 ccggtctaaa ataccaatgc ttagtcattt gtacagcgta gttgtactga atatcaaatt
    10081 gcaactgaga taacattcca ccggcataag atgtatatga aagcaatcct ccaaaagaag
    10141 atctaattgg atgaaaatac tgatcataat ctgctaatat agtctgtgaa ccttcaattg
    10201 ctgaccactg gtttctgtat gttgtataca ccctacctcc accggtaaaa cctgcaaatg
    10261 atggagctat agtagttgta gctgaccacg gctgtgacaa tagaaagtct tgcgctgcca
    10321 agttaaacaa tggcaatatt aatagtacta ctaatgtatt gaattttgct ttcatcaacg
    10381 aactaacata aaattaccag actgacggaa cgatgtaccg tctttaaaat atccttcagc
    10441 cttatacgaa tatgtagctt gttgggcaca tttacctaaa taataaccat cccaaccttg
    10501 atacaaatct tccgacacaa ataccttaac accccattga ttgaatatct ccaatttata
    10561 tgtatctaca ttacgggata caggatagaa tatatcaaga cgacgttctt ccggcgaata
    10621 gactcctcca tttgcctcag ttaaatttgg tacaaatgct gtcgggaatg taatgaatga
    10681 acctttccca actcttacat attcatctaa tttcagagta tctttacaac caaaactatt
    10741 ttcaaccacc aaagatacgt caaacaatcc atcttcacga taaatatatg tcggatcctt
    10801 ttctgtagac tgacctccat ctccaaaatc ccataaataa gagattacct tgtctgactt
    10861 acaataaaag tgagcttctg catttggtgt atataattga gttaagctta cagagaattc
    10921 tgcaaccggc gatacataaa ctgttatatc tttgcttacc tgatctgact ttcttgttcc
    10981 tgtagctgta agtgtaactg tatatgttcc ggactgtgta tatacgtgat ccggacttct
    11041 ttttgtagaa gttgtcccgt ctccgaaatc ccacaaatat tcagcatcac caagtgttgt
    11101 attggtgaat attactgcca atggcttaca accaactata ggatctgcac taaaatctgc
    11161 aataggttca tcaactgcgc gtacttgtaa ttcatcataa gcttcacaat cataagctga
    11221 caatgtccat cgcactgtat taattcccgg agctaaacct gttacatatg tattaggcgc
    11281 agtttcatca tcaaatactc cactgcctgc aactatcgac catcttccgg ttacatttgt
    11341 atcagcaaca gaacgcgctg acaaacgata agatccgttt atcgtagctc catcatctcc
    11401 ggcatcaact aaatgcgtta aactatggga tacaacctct acatcaatag tatttgagca
    11461 agattctgta tagacttccc aacgaatgat atttgtcact ttattactta agtctgtaat
    11521 caaagaaaca ttagatgtag gatcttgtaa tacaccatca ccagatatta gtgtccaacg
    11581 atagtctcca tacaatggtt tcggggcacg caatgtcaca gaatcatcac atattgta
//
LOCUS       DAOPID010000023        11407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_6848_length_11407_cov_4.130902, whole genome shotgun sequence.
ACCESSION   DAOPID010000023 DAOPID010000000
VERSION     DAOPID010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 11407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11407
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6848_length_11407_cov_4.130902"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="PK641_01715"
     CDS             <1..695
                     /locus_tag="PK641_01715"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HPG55106.1"
                     /translation="TKINYPTEFKDYLQSYKPSIGAAIDLGGEYKPLPELSISLSLKD
                     VGFMYWQNSHKAGGDIDYTFTGIEYKPEEQVNYADSLLKALQASYVYDVNASPYVSAM
                     NAKMYVGIEYSFLKNMMSVGLLSRTDLNACRFSEELTLAYNLRPCSWFGLSASYSFIS
                     GGFSTLGLGFNLRLPPFSIYVAGDYMPLYYSKEGIPYRSERLNLQAGIVLTFGCKKCN
                     TKPLYTPTDFAQ"
     gene            complement(752..3169)
                     /locus_tag="PK641_01720"
     CDS             complement(752..3169)
                     /locus_tag="PK641_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HPG55107.1"
                     /translation="MIYPKHFEQKIGFDKIRLLIKNHCISDLGKEIVDEIEFSANFDS
                     VALSMQQTAEFILIIDEGDAFPIDGYFDVRSYLKRIKIEGTFLTESELFEFKRSLFTI
                     KNIINFLELKDAVIYKNLKDLTININYAIDVQRYIDNILDKFGRIKDNASPELTKIRR
                     SKNIAESSISRILSNIIHKAKNDGYLDSDITPTLREGRLVIPISPSYKRKLGGIVHDE
                     SASGKTVYVEPGEVVEANNRIRELEAEEKREITHILQTCAEYIRPFIPDLTESYRFLG
                     IIDFIRAKALFAKQIGGNLPELISETHIEWNHAKHPLLFLSLQSQQKDIVPLDIILNK
                     QNRILLISGPNAGGKSVCLKTVGLLQYMGQCGLLVPMDINSKFGLFKNLFIDIGDEQS
                     IENDLSTYSSHLSNMKTFLREGNEHTLLLIDEFGTGTEPMIGGAIAEAELEVFNQNRC
                     FGVITTHYTNLKHIASQTEGLINGAMLYDRHKMEPLFQLDIGNPGSSFAIEIARKIGL
                     SEQIISNASNKVGLEHINYDKNLQDIVRDKRYWESKRQQIKIKEKKMEENSNALKAQL
                     DTIDKQRKEIIRQAKEEARQLLADTNATIENTIREIKEAKAEKEKTKEIRHKFEETKK
                     AIREDSQSINAQKDQHASNKLSKDKTLNIGDYIKYNDNIGQIISIKGERINVAFGQLE
                     AVLNIKMLEKIGRKQVKEQQTKINYNFVSPETNEEIRKRKLRFKQDIDVRGQRVDKAV
                     LTITYFIDDAILVGVSSVRILHGTGTGALRQAIRDYLSTVSGVKSFRDEHIQLGGAGI
                     TVVELKD"
     gene            complement(3147..4259)
                     /locus_tag="PK641_01725"
     CDS             complement(3147..4259)
                     /locus_tag="PK641_01725"
                     /inference="COORDINATES: protein motif:HMM:NF037043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPG55108.1"
                     /translation="MTKDSSENRIYEAEQLEGKAIANIKIPSWAAIDNIKYPKRLSME
                     QCSSEYTAKYKANLVQGDSFTDLTGGFGVDFAFMSQSFKKATYNEQNTDLFNIANHNF
                     KLLQLNNVQTINYDAIEYLNSIKKQNCIYIDPARRSKSGRKMIRLEDCEPDLTQIKDK
                     LLSKCDTLIVKLSPMLDMDAALKNLPETSDIHVISIKNECKELIFVLQTNNEHASTQI
                     HCVNILEKPCFTFTKDEEKNAQSFFSMPLKYLYEPNSSILKAGAFKMVGIKYNAYKLH
                     INSHLYTSKELIDSFPGRCFEIMEICSAHKNKLKHIQKANLAIRNFPSSVDELQKKIG
                     IKDGGELYLFATTILDGSKNILVCKKVNKNDISEAL"
     gene            complement(4256..5716)
                     /locus_tag="PK641_01730"
     CDS             complement(4256..5716)
                     /locus_tag="PK641_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005775588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HPG55109.1"
                     /translation="MKLLDIKIVIRIMSFLLLFETFFMLISAFVSIAYGESDYQYIFY
                     SSGVTLGFSLLGILLTPKIKVEMGKREGYLIVALVWIVFSLFGLLPFYWSGSIPSFTD
                     AFFETMSGFTTTGASILPEVEKLSYGLLFWRSIIQWLGGMGIIVLSLALLPLLGVGAM
                     PLFVAEVPGPTKDKLHAKISSTAKILWGVYCGITLIQTLCLWSAGMNLFDSVCHAFTT
                     MATGGFSTKNASIAYWNSPLIEYIIILFMFVASINFSLYFFLLKGNFRKFFHNEEFKY
                     YTIFAFVLSLIVFVALTIKHSETFGIEERIRYSLFNVISLMSTTGYVNVDYIQWHQFI
                     WLFLLVVMLFGGSAGSTAGGIKTVRIVILIKNSYYEFKRLIHPNAVIPVRFNDEVLKP
                     QLINNVLAFFFLYSVITIVGILFFVACGMGLVESIGTAVTAIGNVGPGLGIVGPVSNF
                     GTIPMACKWCASALMLIGRLELFTVLMVFTQAFWKK"
     gene            complement(5738..7069)
                     /gene="trkA"
                     /locus_tag="PK641_01735"
     CDS             complement(5738..7069)
                     /gene="trkA"
                     /locus_tag="PK641_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HPG55110.1"
                     /translation="MKIIIAGAGDVGTHLAKMLSKESHTIVLLDEKAEKIKALEANYD
                     ILAIVGSPTSLKDLREAQVPTADLFVAVTPYESTNITACLLATNLGAQKTLARIDSNE
                     YLLPKNKEFFKSMGVDSLIYPEMLAANEIVNGLKKGWVRQYVEFSDGALIVLATKIRA
                     NSPLCNVPLEKAFEGNGLVRIVVIKRGNVTIIPTGKDSVQNNDIAYFITTKEGVEEVR
                     IKSGKEDFDINNIMFVGASRIGIKTIQNLPKGKDVKVIEQDIDKTYRIIEQTNNTLVI
                     NGDGRDLSLLKEEDIESFDAFVALTGNSETNILACLTAKQFGVKRTIAEVENMDYIPM
                     AESLDIGSIINKKIIAASHIYQLILGGNVSVRCLTFANVEVVELVAEEGSYITTDCLK
                     NLKLPKNTTIGGIVRYNKGIVAVGDTKIKAGDNVTVFCGEYTIQKIEKLFK"
     gene            complement(7107..7562)
                     /gene="smpB"
                     /locus_tag="PK641_01740"
     CDS             complement(7107..7562)
                     /gene="smpB"
                     /locus_tag="PK641_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018712066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HPG55111.1"
                     /translation="MDSKIYIKNKRASFDYELLDKYTAGIVLVGTEIKSIRMGKASLV
                     DSYCYFDKGELWIRSMNITAYFYGSYNNHNPQRDRKLLLSKKELRKLERGVKDPGVSI
                     IPTALFINEKGLAKVNIALAKGKKEYDKRQSIKENEDKRKMAQASQHSY"
     gene            complement(7567..>8106)
                     /locus_tag="PK641_01745"
     CDS             complement(7567..>8106)
                     /locus_tag="PK641_01745"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HPG55112.1"
                     /translation="TFFTFIINPAKAWQDVSNKESGILNSNTYAYILLGLTSLFAFIG
                     SAIHAEDFNWQLGIKEASLLFISLFIGIYLSTFVVRILHEHICKNPISFNTGIEFTIY
                     SFSCIFLINMAGELIRDIFYIQIVALYTIYIVWEGSINYLSVPNNKRTLFVVCTSGTI
                     ILSPILIRYLMVLLMPGME"
     assembly_gap    8107..8116
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8265..9329
                     /locus_tag="PK641_01750"
     CDS             8265..9329
                     /locus_tag="PK641_01750"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55113.1"
                     /translation="MIYLFTVILLLASLYIYFPIAKKYRLLAGVNNRSSHKRPVITGA
                     GFIFYLSYLVYIVHQIWTGDQIPIWLFIGLTILAVISFMDDLSDVWFLFRLMVQGVAA
                     IALLWQLKCDFDIDVQASVSQWLAACFLLILAVGSFNLYNFMDGLNGMLGGLALSMVL
                     PALLIDLYVVDFIDDELLYFTIIPTIIFLFFNYRKQPKCFSGDVGSIVLGYLMVYMVL
                     SLLIKTGNVVYIFFFSVVYIEAGLTVFQRLFAGENIFKPHRIHLFQLLCNEHNKSHRL
                     VSEIYAGIQFLMGIGLFLLNYYDVPRLYQNIIFWPIFGGLAFFYLWYKRKRMGGHLLE
                     YNENTTNIKDQFKKIKKIKV"
     gene            9326..9742
                     /gene="mce"
                     /locus_tag="PK641_01755"
     CDS             9326..9742
                     /gene="mce"
                     /locus_tag="PK641_01755"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HPG55114.1"
                     /translation="MIPEHIEHIGIAVPSIEEALPYYEKVLGMKCYSIEEVADQKVRT
                     AFLKVGEVKLELLEPTSPESTIAKFIEKNGGRGGVHHIAYSVKDIEDKLKQVEENGVQ
                     LIDKTPRGGAEGLSIAFLHPKSTAGILTEFCENKNK"
     gene            9799..11360
                     /locus_tag="PK641_01760"
                     /pseudo
     CDS             9799..11360
                     /locus_tag="PK641_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776335.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
BASE COUNT         3503 a         1902 c         1944 g         4048 t
ORIGIN      
        1 taacaaaaat taattatcct acagaattta aagactactt gcaatcatac aaaccaagta
       61 taggtgctgc tatcgatttg ggcggagagt acaaaccatt acccgagttg agcatctcat
      121 tgtcattgaa agatgttggt tttatgtact ggcaaaattc gcataaagca ggtggtgata
      181 ttgattatac atttacagga attgaatata agcctgaaga acaggtaaat tacgcagatt
      241 cattgcttaa ggctttgcaa gcgtcttatg tatatgatgt taacgcaagt ccttatgtgt
      301 ctgcaatgaa tgcaaaaatg tatgtaggta ttgaatactc atttttaaag aatatgatga
      361 gtgtaggttt gctgtcgcgt acagatctta atgcatgtcg cttttcagag gaattgactt
      421 tagcgtataa tttaagacct tgtagctggt ttggtttatc ggcaagctat tcatttatca
      481 gtggaggttt ttcgacctta ggtctaggat ttaatcttcg cttaccgccg tttagcatat
      541 atgttgcagg agattatatg cctttatatt attcaaaaga aggtatacca tatcgttccg
      601 aaagacttaa tttacaagcc ggtatagttc tcacattcgg atgcaagaaa tgtaatacaa
      661 aaccattgta tactccaaca gattttgctc aataatcaaa cataaactat tatagcaaaa
      721 aacagaaggt gttagccttc tgttttttgt tttagtcttt tagttcaact actgtaatcc
      781 cggcaccgcc taattgtata tgttcatcgc ggaatgactt tactcctgaa actgtcgata
      841 aataatcgcg tatggcctgt cgtaatgctc ccgttcctgt tccatgtaaa attctaacag
      901 aacttacacc aacaagaatt gcgtcatcaa taaaataagt tatggtcaat acggctttgt
      961 caactctttg tccacgaacg tctatatctt gcttaaatct taatttgcgt tttcttattt
     1021 cttcattggt ttcaggactt acaaaattgt aatttatctt cgtttgttgc tccttaactt
     1081 gtttacgtcc tattttttca agcattttaa tatttaaaac agcttctaat tgaccgaaag
     1141 caacattgat tctttcgcct ttaattgaaa ttatttgacc tatattgtca ttgtatttta
     1201 tgtaatcgcc aatatttaag gttttatctt tagatagttt gttcgatgca tgctgatctt
     1261 tttgtgcgtt aatgctttga gaatcttctc ttatggcttt ttttgtttct tcaaacttat
     1321 gtctaatttc tttagtcttt tctttttcgg ctttggcttc ttttatctcg cgtatggtat
     1381 tttcgatagt agcatttgta tcagcaagta attgcctggc ctcttctttt gcttgacgta
     1441 ttatttcttt tctttgttta tcaatagtat ctaattgtgc ctttaaagca ttggagtttt
     1501 cctccatctt tttctctttg atttttattt gttgacgttt gctttcccaa tagcgtttat
     1561 ctcgcacaat atcttgtagg ttcttatcat aatttatatg ttctaaaccc accttgttgc
     1621 ttgcattaga tattatttgt tccgacaaac ctatttttct tgcaatttca atagcaaatg
     1681 aactacccgg attacctatg tctaattgaa aaagaggttc cattttatgc ctatcgtata
     1741 acattgcgcc attaatcagc ccttctgttt gtgatgctat atgctttaaa ttagtgtagt
     1801 gcgtggttat cacaccaaag catctatttt gattaaaaac ttctaattct gcttctgcaa
     1861 tggccccacc aatcattggt tctgtacctg taccaaattc gtcaattaat aataaagtat
     1921 gttcgttacc ttctctaaga aaggttttca tattacttaa atgtgaactg taagtactta
     1981 aatcattttc tatagattgt tcatcaccaa tatcaataaa aagattcttg aataaaccga
     2041 atttactatt tatatccatt ggtactaaca gaccacattg acccatatac tgtaataatc
     2101 ccacagtttt taagcaaaca gattttcctc cggcatttgg acctgaaata agcaaaatac
     2161 gattttgttt attaagtata atgtcaagtg gaactatatc tttttgttgg ctttgcaaac
     2221 tcaaaaatag caatggatgt tttgcatgat tccattcaat gtgagtttct gaaatcagtt
     2281 caggtaaatt tccgccgatt tgtttggcaa acagtgcctt agctcttatg aaatctatta
     2341 taccaagaaa tctgtatgat tcggttaaat caggaataaa aggtcttatg tattctgcac
     2401 atgtctgcaa aatatgtgtt atttcgcgtt tttcttctgc ctctaattcg cgtattctgt
     2461 tgttagcttc aacaacctct cccggctcta catacaccgt tttgcctgat gctgattcgt
     2521 catgtacaat accacctaat ttacgtttgt atgagggtga tataggtatt acaagtctac
     2581 cttcacgtaa agtaggagtg atgtctgaat ctaaatatcc atcattttta gccttgtgaa
     2641 taatattgct aagtatgcgt gatattgaag attctgcaat gtttttactt ctacgaattt
     2701 tagttagttc gggtgatgcg ttgtctttaa tccttccaaa tttatccagg atgttatcga
     2761 tatacctttg aacatcaatt gcgtaattaa tattgatagt taaatcttta agatttttat
     2821 aaattactgc atcctttaat tcgaggaagt taatgatgtt ttttattgta aaaagagaac
     2881 gtttaaactc aaatagttct gattcagtta aaaaagtccc ttcaattttt atgcgtttaa
     2941 gataactgcg aacatcaaaa tatccatcaa tcgggaaagc atctccttcg tcaataatga
     3001 gtataaattc tgctgtttgt tgcatgctaa gtgcaacaga atcgaagttt gcagagaact
     3061 ctatttcatc aacaatctct tttcctaaat cacttatgca gtgatttttt ataagtagtc
     3121 tgatcttatc gaaccctatt ttctgttcaa agtgcttcgg atatatcatt tttatttact
     3181 tttttacaaa ccaaaatgtt ttttgaacca tctaatatgg tcgttgcaaa tagatacaat
     3241 tcgccaccat cttttattcc aatctttttt tgaagttcat caaccgatga aggaaaattt
     3301 ctaattgcaa gattagcttt ttgtatatgt tttaatttgt ttttatgtgc gctacaaatt
     3361 tccattattt caaaacagcg accaggaaac gaatctataa gttcttttga tgtgtataga
     3421 tggctgttta tgtgtaattt atatgcatta tattttatac ctaccatttt aaatgccccg
     3481 gctttcagta tgcttgaatt cggctcgtat agatatttta aaggcattga aaaaaaagat
     3541 tgtgcatttt tttcttcgtc ttttgtgaat gtaaaacatg gtttttcgag aatatttaca
     3601 caatgaattt gagttgatgc atgctcattg tttgtttgta atacgaaaat taattctttg
     3661 cactcgtttt ttattgatat aacatgtata tctgatgttt ccggtagatt cttgagtgct
     3721 gcatccatgt caagcattgg agatagttta actataagag tatcacattt actcagtagt
     3781 ttatctttta tttgagttaa atcgggctca caatcttcca aacgtatcat ctttctgcct
     3841 gatttactac gtcttgccgg gtctatgtaa atgcaatttt gttttttaat tgaatttagg
     3901 tattcaattg catcataatt aatagtttgc acattattta gttgcaatag cttgaaatta
     3961 tgatttgcga tattaaataa atcagtattc tgctcgttgt aagttgcttt tttaaatgat
     4021 tgactcatga aagcaaaatc aactccaaat ccccctgtca agtctgtaaa cgaatctccc
     4081 tgaacaagat ttgccttgta ttttgccgtg tattctgacg agcattgttc catcgacaaa
     4141 cgtttaggat atttaatatt atcaattgca gcccaacttg gaatttttat gttagctatt
     4201 gcttttcctt ctaattgctc tgcctcgtat attctatttt ctgaggagtc ttttgtcatt
     4261 ttttccagaa tgcttgtgta aataccatta gtacggtaaa taactcaaga cgcccgatta
     4321 acattaatgc agatgcacac catttacaag ccattggaat tgttccaaag tttgatacgg
     4381 gtccaactat tcctaagcct ggtccgacat taccaatagc agtaactgca gtacctattg
     4441 attctacaag acccatacca caggccacaa aaaataaaat gccaactata gtaataacag
     4501 agtataaaaa gaagaatgcc aatacgttat taataagttg cggtttaagt acttcatcgt
     4561 taaatctaac aggaataaca gcattcgggt gtatcaatct tttaaattcg taatagctgt
     4621 ttttgattaa aataacgatt ctaacggttt tgataccgcc tgctgtagaa ccggcactgc
     4681 cgccaaataa cattaccact aaaagaaata accaaataaa ctgatgccat tgaatataat
     4741 cgacgttcac gtatccggtg gttgacatta gtgatataac attgaacaat gagtatctta
     4801 ttctttcttc tataccaaat gtttcactat gtttaatagt caacgctaca aatactatta
     4861 atgataaaac aaatgcgaag atagtgtaat atttaaactc ttcgttgtga aagaactttc
     4921 taaagtttcc cttaagtaaa aagaaataaa gagaaaaatt aatacttgca acaaacataa
     4981 ataatatgat aatgtattct ataagaggag aattccagta tgcgatactg gcattttttg
     5041 ttgagaatcc gccggttgcc atagtagtaa atgcatgaca tactgaatca aataaattca
     5101 tacctgcaga ccataaacat aatgtttgaa ttagtgtaat tccgcagtac accccccata
     5161 aaatttttgc cgttgaacta atctttgcat gtaatttatc ttttgtaggg cccggaactt
     5221 ctgcaacaaa taatggcata gcaccgacac caagtagtgg taataatgct aatgataaaa
     5281 ctataatacc catacctccc aaccattgaa tgatactacg ccaaaatagt aatccataag
     5341 ataatttttc aacctcaggc aatatacttg ctccggttgt tgtgaatccg gacattgttt
     5401 cgaaaaaagc atccgtaaat gatggaatag agccactcca ataaaatggt agtaaaccga
     5461 atagagaaaa tacaatccaa actaacgcta caatcaaata accctctcgt ttacccattt
     5521 cgacttttat tttaggagtt aaaagtattc cgagtaaact gaatcctaat gtcactcccg
     5581 aggaataaaa aatgtattgg taatccgact ctccgtaagc tattgataca aatgcagaaa
     5641 taagcataaa gaaagtttcg aacaagagta agaaactcat tattcgaatt actattttta
     5701 tgtctaataa tttcataata taaagtttta tgcgcgttta tttaaatagt ttttctattt
     5761 tctgtatggt atactcaccg caaaatacag ttacgttgtc tccggctttt attttagtat
     5821 caccaacagc aacaatacct ttattatatc tcacgatacc tccaatagtt gtatttttgg
     5881 gtaacttcaa attttttaaa caatctgttg tgatgtatga accttcttct gccactaatt
     5941 caacaacctc tacattagca aatgtaagac atctgacaga aacattgcca cctaagatca
     6001 gttgatatat atggcttgca gcaattattt ttttgtttat aatagaacct atatccaaac
     6061 tttctgccat tggtatatag tccatatttt cgacctctgc aattgtccgt ttaacgccaa
     6121 attgtttagc tgttaaacag gcaagtatgt tggtttccga attgccggtt aatgcaacaa
     6181 aagcatcaaa cgattctata tcttcttctt ttaacagact taaatctctg ccatccccat
     6241 ttataactaa agtattattt gtctgttcta ttattctgta tgttttgtca atatcctgct
     6301 caattacctt aacatctttc ccttttggta aattttgaat tgtttttatg cctattcgac
     6361 ttgctccaac aaacattatg ttattgatat caaagtcttc tttgcctgat ttaatacgaa
     6421 cttcttcaac gccttcttta gtagttatga agtatgctat atcattattc tgaacagaat
     6481 ctttacctgt tgggataatt gtaacatttc ctcttttaat aactacaata cggacgagtc
     6541 cattaccttc aaaagctttt tctaatggaa cattgcacag gggagaatta gctcttattt
     6601 ttgtggctaa aactattaaa gcaccatctg aaaattctac atattgacgt acccaacctt
     6661 tttttaatcc atttacaatt tcatttgcag caagcatttc aggataaatc aaagaatcga
     6721 ctcccatact tttgaaaaac tctttatttt ttggaagcag atattcgttg ctatcaatac
     6781 gagcaagtgt cttttgtgct ccaagattgg tagctaataa gcaagctgtt atatttgtac
     6841 tttcgtatgg agtaactgca acaaataggt ctgcagtagg tacttgtgct tcgcgcaaat
     6901 cttttaatga agtaggcgaa cctacaatcg caagaatatc ataattagcc tccaaagcct
     6961 ttattttctc ggctttttca tctaataata ctattgtgtg gctttcctta gaaagcattt
     7021 tggcaagatg agttcctaca tcaccggctc cggcaattat aattttcatg ataagtttag
     7081 gtttttaaaa taatattttg ccttatttaa taactatgtt gtgaagcttg cgccattttg
     7141 cgtttgtctt cattttcctt aatagactga cgtttgtcgt attctttttt gccctttgct
     7201 aaggcaatgt taacttttgc cagtcctttt tcattgataa ataatgccgt aggtataatt
     7261 gaaacccccg gatcttttac tcctctttct aattttctta gttctttttt tgacaacaat
     7321 aatttgcggt ctctttgtgg attgtgatta ttatatgaac cataaaaata tgcagtgata
     7381 ttcatacttc taatccataa ttcgcccttg tcaaaatagc aatatgaatc aactaaactt
     7441 gcttttccca tacgaataga ctttatttct gtacctacaa gtacaatgcc cgcagtgtat
     7501 ttatcaagta gctcataatc gaaagatgca cgtttgtttt ttatgtaaat ctttgaatcc
     7561 atatttttac tccattcctg gcataagtaa taccattaaa tatcttataa gaataggcga
     7621 taaaataatg gttcctgatg tgcaaactac aaatagtgtg cgcttattgt ttgggactga
     7681 caaatagtta atagagcctt cccaaacaat gtaaattgta tacaaagcaa caatttgtat
     7741 ataaaaaata tctctaatta attcacctgc catattgatt agaaagatac atgaaaagga
     7801 gtatatggta aattcgatac ctgtattaaa tgatattggg tttttacaaa tatgctcgtg
     7861 caggattcta acaacaaagg ttgaaagata tatacctata aacaaagata tgaataataa
     7921 agatgcttct tttataccta attgccaatt aaaatcttca gcatgaattg cagaacctat
     7981 aaacgcaaat aaagaggtaa gtcctaatag tatataggca tatgtattag aattaagaat
     8041 ccctgattct ttatttgaaa catcttgcca agctttcgca gggtttatta tgaatgtaaa
     8101 aaaagtnnnn nnnnnnataa tatgttttta tacataacta aatgagtctt tattatgcat
     8161 tgcaaagata aggaaaaatt atacattatt ttgttcgctc aataagatat ttatttgtac
     8221 ttttgccgat taattcgttt ttatccccct aaaaaaagaa tgtaatgatt tatttgttta
     8281 cagtaatcct tttacttgcg tctttgtaca tatatttccc gatagcgaaa aaatatcgct
     8341 tgcttgccgg agttaataat agaagttcgc ataaaaggcc cgtgattaca ggtgcaggtt
     8401 ttatttttta tttgtcttat ttggtgtata tagtacatca aatatggaca ggagatcaaa
     8461 tcccaatttg gttgtttata ggattgacca tattagcagt aattagtttt atggatgatt
     8521 tgagcgatgt ttggtttttg tttcgcttaa tggtacaagg tgttgcagct atagcattgc
     8581 tgtggcagtt aaaatgcgat tttgatattg atgtgcaagc atctgtatct cagtggcttg
     8641 ctgcttgctt tttgctaatt ttagcagtag ggtcatttaa tctatataat tttatggatg
     8701 gtcttaacgg catgcttggt ggtcttgctt tatcaatggt attgcctgct ttactgattg
     8761 atttgtatgt agtagatttt attgatgacg aacttcttta ttttacgatt ataccaacaa
     8821 ttattttctt attttttaat tatagaaaac agcctaagtg tttttccggt gatgtcggct
     8881 ctatagtgct cggttattta atggtttata tggttttgtc tttgcttatt aagacaggta
     8941 atgttgtgta tatattcttt ttttcagttg tttatataga ggccggtttg acagtgtttc
     9001 agcgattgtt tgccggcgaa aatattttca aacctcatag aatacacttg tttcaattgt
     9061 tgtgtaacga acataataaa tctcatcgat tggtgtccga aatatatgca gggattcagt
     9121 ttttaatggg tatagggttg tttttgttaa attattatga tgttcccaga ttatatcaga
     9181 atattatatt ttggcctatt tttggtggat tagcattttt ttatctgtgg tacaaacgta
     9241 aacgcatggg cggacatttg ttggaataca atgaaaatac aactaacata aaagatcaat
     9301 ttaaaaaaat taaaaaaata aaagtatgat tcctgaacac attgagcaca taggtatagc
     9361 agtgcctagt attgaagaag cattgcccta ttatgaaaaa gtattgggca tgaaatgcta
     9421 ttctattgaa gaagtagccg accaaaaagt tagaactgct tttttgaaag ttggagaagt
     9481 gaaattggaa ttgttggagc caacaagtcc tgaaagcacc atagcaaaat tcatagaaaa
     9541 gaatggagga cgcggtggtg ttcatcatat tgcttattca gtaaaagata ttgaggataa
     9601 attgaaacaa gttgaagaaa atggagttca attaattgat aaaactcctc gtggtggcgc
     9661 agaaggcctg agtatagcat tcttgcatcc aaaatcaaca gcaggtattt taaccgaatt
     9721 ttgtgaaaac aagaacaaat aactaaaata tgagttttgt ggaaacgaag cttatatact
     9781 attaatatta aattaattat ggaagaaaat caagacaaaa tcaagaaatt gattgagaag
     9841 cgagaagctg ctcgtatggg tggaggcgca aagagcatcg aaaaacatca tgctaaaggt
     9901 gcttttacag cacgtgaaag aatcaacatg ttgctagatg agggtagttt tgaagaagtt
     9961 gatatgtttt tgacacatcg ttgtaccgat tttggaatgg acaagaaagt attcttgggt
    10021 gatggtgttg cagtaggtag tggtacaata gatggtcgtt tggtattcat atttgcacaa
    10081 gatgcaactg taataggtgg ttcattgtca gagactatgg ctcagaagat atgcaaagtg
    10141 atggatatgg cgatgaaatt aggagctcct gtgattggat taaacgattc aggtggtgct
    10201 cgtatacaag aaggtgcatg tgccttagca ggatatgctg aaatttttga acgcaatatc
    10261 ttagcatcag gtgtaatacc tcaaatttca gtaatttttg gaccttgcgc aggtggagca
    10321 gtttattctc cggctttaac agactttatt ttgatgacag aaaaaaacag ctatatgttt
    10381 attaccggac caaaggttgt taaagctgtt acaaatgagg atgttaccgt agaacaatta
    10441 ggtggagcaa gaattcatgc aacaagatca ggagtaactc actttactgc tcctacagag
    10501 gaggaagctc ttgatgttgt tcgtcgtttg atttcgttta ttccacaaaa caatatggaa
    10561 gaggttcctg ttcagccatg tacagatcct atagatcgtg ctgatgaagc tttaaatact
    10621 attgttcctg aaaatccaaa taaaccttat gacatgaagg aaattatttc tacaatagta
    10681 gacaatggtg actttatgga ggttcaacct gaatatgctc aaaatattat ttgttgcttt
    10741 gctcgtttta atggcaaatc aaccggcatt attgcaaatc aaccatgctt aatggctggt
    10801 gttttagatt gcgatgcatc acgtaaagca gctcgttttg tgcgtttctg cgatgcgttc
    10861 aatattccga ttttgacatt ggtagatgta cccggattct tgtgtggaac aggtcaagaa
    10921 tatgccggaa ttattgctca tggcgcaaaa cttatgtttg catatggtga agcaacagtt
    10981 cctaaggtta ctgtaacaat tcgtaagtct tatggtggta gccatatcgt tatgagctgt
    11041 aagcaactac gaggtgatat gtgttatgca tggcctaatg cagaaattgc agtaatgggc
    11101 gcatctggtg caattgcgta ttggaagcta aggcattgag tgcaatagaa gatcctaaag
    11161 aaaaagctaa attcatagca gaaaaagaag ctgagtacca agataaattt gcaagtccat
    11221 atcaagctgc ttctcgcggt tatattgacg atgtaattga acccataaat actcgtttcc
    11281 gtattattcg tgcgtttgag atgttgcaaa caaagcgttt gacgaatcca ttgaagaaac
    11341 attcaaacat acctttataa tctaaaacta tggtattagc agttaattgg ggcaatgccc
    11401 ttataat
//
LOCUS       DAOPID010000024        11055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_7187_length_11055_cov_4.626545, whole genome shotgun sequence.
ACCESSION   DAOPID010000024 DAOPID010000000
VERSION     DAOPID010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 11055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11055
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7187_length_11055_cov_4.626545"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1279)
                     /locus_tag="PK641_01765"
     CDS             complement(<1..1279)
                     /locus_tag="PK641_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55115.1"
                     /translation="MSACSTTKYVPEGQYLLNKVSIKTDKKDLKGVNMLTYLRQRPNT
                     KLFDFIPFNVALYSLSGRDTTQWINRFLQKIGDAPVIYDPALTSTSEKELKKHLANKG
                     YENAFVSSNATFKKKKVSVEYIIKANEPYRIRNFKYRINNPEIKELIYKDSVNRTIHS
                     GELFDTDALEAERGRLSEYLKNNGYYYFDKEYIGFIADSSLNTHQVDLLMVVRPLRKV
                     VGKDTYKSEEHKTYRIRNINYYTVNEPTDLLNDSILQSLESIKYKDRFVYSNIKFIRP
                     SALYNKTYIDPDSLYSAKAVDQTYAKLSALNVFKYLDISFSEVLDSSVIDSTASDSLK
                     DMMMDCDIVVTPDKSQSFSFEVEGINNEGDFGIAGKFGYKHNNIFRGGESLKFQLRGA
                     NESIIGTRSIWDISTEIGLSFPNSFLRNGKKKFG"
     gene            1415..2155
                     /locus_tag="PK641_01770"
     CDS             1415..2155
                     /locus_tag="PK641_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPG55116.1"
                     /translation="MLSKNRIKYIKSLSIKKNRYEEKCFVAEGSKTVEDLLSSFVCRA
                     MYGTERYIGIRPDCFELITPAELKSISNLETPQDVLALFEMPENICDSMDVSGKLSLA
                     LENIQDAGNLGTIIRVADWFGIENIMCSIGTVDVYNCKTVQATMGALARVNVYYCDLK
                     TCLMKLSSEMPIYATSLDGADIYAAELSKNGVVVMGNEGNGISYELKQICNSKLLIPN
                     YPKGRKTSESLNVGIATAVICAEFRRPR"
     gene            complement(2090..2584)
                     /locus_tag="PK641_01775"
     CDS             complement(2090..2584)
                     /locus_tag="PK641_01775"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HPG55117.1"
                     /translation="MKSCISIIIVMLALGLSSCKQDVNKPDFMPEQDKMASLLTEIHI
                     TEALTRYNYIQDRRKLNNYYKEILDNYGLSPEQFDSCIAWYSINNKEYKELYNIVINN
                     LEAEKQLILSRYYSKERPKSIWQNLDSIQQINGFRNWNYNKLSRPAEFGANDSCCNSY
                     VERF"
     gene            complement(2581..3135)
                     /locus_tag="PK641_01780"
     CDS             complement(2581..3135)
                     /locus_tag="PK641_01780"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HPG55118.1"
                     /translation="MSNQTKKALFSAFIIFLILLIDQVSKYLVKANLYLGEELYIFSW
                     FRIHFLENEGMAFGMSFFDKKYLTLFRIVASGLILWYLIHCIKKNESLLLLSCLALIF
                     AGAFGNIIDCIFYGQIYGYAPIFYGKVVDMLYFPLIEGRFWSWIPYFGGESFIFFSPV
                     FNIADSAICIGVALLLIFERRIFK"
     gene            complement(3136..3513)
                     /locus_tag="PK641_01785"
     CDS             complement(3136..3513)
                     /locus_tag="PK641_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HPG55119.1"
                     /translation="MAEKTRYSDAELEEFRVIINDKLQQAYVDYEQLRNALNNLDGND
                     TSDTSPTFKVLEEGATTMTKEEAGRLAQRQMKFIQHLQAALVRIENKTYGICRETGKL
                     IPKERLRAVPHATLSIEAKTEKK"
     gene            complement(3575..6931)
                     /gene="ileS"
                     /locus_tag="PK641_01790"
     CDS             complement(3575..6931)
                     /gene="ileS"
                     /locus_tag="PK641_01790"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HPG55120.1"
                     /translation="MNKHFAEYQGIKLSEINKEILEQWDKNNIFQKSIDTRKGHPSFV
                     FYEGPPSANGMPGIHHVMARTIKDLFLRYKTMKGFMVERKAGWDTHGLPVELGVEKSL
                     GITKEDIGKTISVEEYNSICRKDVMKYTKEWESLTHKMGYWVDTNNPYITYENSYIES
                     IWWLLQQLYKQGLLYKGYTIQPYSPAAGSGLSSHELNQPGCYKDVKDTTVTAQFKVKG
                     EKNLYFLAWTTTPWTLTSNTALCVGPNIDYVRYATQNPYTKEDVELILAADLFDSYFK
                     GQEKPSETFKGSTLVGMEYEQLIPFFKADEGAFRVIAGDYVTTEDGTGIVHIAPTFGA
                     DDARVAKMAGIPAMLLIDKLGNQRPLVDLQGRFYRIEDIDLTYLKDRVNIEDYKQFAG
                     RYVKNAYDSSLTDKDETLDISICMMLKQQGLAFKIEKHVHNYPHCWRTDMPVLYYPLD
                     SWFIKSTARKDEMFKLNETIKWKPQSTGSGRFGKWLENLQDWNLSRSRYWGTPLPIWR
                     TEDGKEEICIGSIAELISEIEKSVKAGFMTENPYKNFKVGDFSKENYDCKNIDLHRPY
                     VDNIILVSASGKAMKRELDLIDVWFDSGSMPYAQLHYPFENKDLIDNGTRFPADFINE
                     GVDQTRGWFFTLHAISTMVKHSVAFKNVISSGLVLDKNGNKMSKRLGNAVDPFFTIDK
                     YGSDPLRWYMMTNASPWDNLKFDTDGVEEVSRKFFGTLYNTYSFFALYANVDNFTYSE
                     KDVPVSERPEIDRWIISLLNSLIKEVDESLADYEPTRAGRAISDFVTENLSNWYVRLN
                     RKRYWAGSMDKDKLSAYQTLYTCIETVARLMAPIAPFYADRIFLDLNAATHKNTKESV
                     HLADFPTYDASLIDSKLESQMDVAQRITSLVLALRKKANIKVRQPLSHIMIPSTNAEL
                     ETNLKAVENLIKAEVNVKEIKLVDNSAGILVKRIKPDFKKLGPKCGKDMKQVAQLLQN
                     MSQTDITKFEDEGQFEYTINDTRIYILTDDVEIISEDIPGWLVANEGKLTIALDITLT
                     DDLISEGIAREAINRIQNLRKSTGLEITDKIKIKIQSDKNFDSAIEKHKSYIASQVLA
                     TSIEIVSELPNGQAIEMDDNKIILDIKKAM"
     gene            complement(7027..7440)
                     /locus_tag="PK641_01795"
     CDS             complement(7027..7440)
                     /locus_tag="PK641_01795"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HPG55121.1"
                     /translation="MIQRIQTVYLFIAFVLGTLMCFFPVAHVAEHTVGFGSFMPYSVL
                     CILIPLISLITIFIYKKRILQIRFTSFNIILMVFQLLLIVLCIYLLYKNNNADASIKI
                     SWPIVILPINIILSYLAIREIGKDEALVHSMERLR"
     gene            complement(7564..7749)
                     /locus_tag="PK641_01800"
     CDS             complement(7564..7749)
                     /locus_tag="PK641_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55122.1"
                     /translation="MKKLVLFAAAIVAVAFASCNKEAAQEEAEEAVVEEVVAPVEEVI
                     VDSAAAVVADSAAVVAE"
     gene            complement(7892..9559)
                     /locus_tag="PK641_01805"
     CDS             complement(7892..9559)
                     /locus_tag="PK641_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005790938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPG55123.1"
                     /translation="MIEQNFIKLFEHSFKENWDYPIYTNYYKNQTFKYSEAANQIAKL
                     HLLFESAKVEQGDKIALIGRNTPNWTITYIAVVTYGAVIVPILQDFNPNDVHHIVNHS
                     ESKMLFCSDKIWDTLEEEKMNNLIAVFSLNDFRCIHQKDGEYIQKIMNSLTDNFNKRY
                     PQGYKQEHVKYTERDNAELAMISYTSGTTGFSKGVMISGNALAGNITFGIRTHLLERG
                     DKVLAFLPLAHAYGMAFDFLTATCVGSHTYLLNKIPSPKVLMKAFEEIKPKVIFTVPL
                     IMEKIYRKQLQPALNKMSMRLALSIPLLDDKIYQQIKKKLNEAFGNEFREVIIGGAPL
                     NKEVEDFLRKIGFKFTVGYGMTECAPLICYSPHDKFVPYSAGKILDIMEIMIDSKDPY
                     NIVGEICVRGENVMSGYYKNVEATKNIFKEDGWMRTGDMGTIDKEGNLFIKGRCKSMI
                     LGASGQNIYPEEIEEKLNNLPFVMESLVIDKDGKLLGLVYPDYEAVDASNISHSDLDI
                     IMEENRKQLNSMVANYENISKIILYPNEFDKTPKRSIKRYLYTNLNI"
     gene            complement(9848..>11055)
                     /locus_tag="PK641_01810"
     CDS             complement(9848..>11055)
                     /locus_tag="PK641_01810"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HPG55124.1"
                     /translation="GRILSENGFICDQDLICSDIDIERLRYDRMKQSSYADAKSSYYT
                     PVRYISSNIDLKPDQIVYPIERRPFVPNNGPEKDERCSEIMDIQVAGLKKRLEHINCN
                     NVIIGISGGLDSTLALLVATKAFDELGYDRKNIMGVTMPGFGTTSRTMGNAIKLMESL
                     NTTSITISIKDACIQHFKDINHDISVHDICYENTQARERTQILMDMANKTNGIVIGTG
                     DLSELALGWCTYNGDHISHYGVNVGVPKTLVRSLVNAYATLHDTKTKAILADIIDTPI
                     SPELLPKGDNDEIVQKTEDVVGPYELHDFFIYYMLRFGFSPSKIMALAETAFSEKEVS
                     DDVLYEKATILKWLKICYKRFFSQQFKRNCLPDGPKIGSIALSPRGDLRMPSDASANI
                     WLKELNGLE"
BASE COUNT         3345 a         1930 c         1847 g         3933 t
ORIGIN      
        1 ttccaaactt ctttttcccg tttctcaaaa aggagtttgg aaagcttaat cctatttctg
       61 tagaaatatc ccaaatagat ctggtaccta ttatcgattc gttggctccg cgcaattgga
      121 atttaagaga ttcgcctcct ctaaaaatat tgttatgttt atagccaaat ttaccggcaa
      181 taccaaaatc accctcgtta ttaattcctt ctacttcaaa actgaatgat tgtgatttgt
      241 caggagtaac aacgatatcg caatccatca tcatatcttt taaagaatct gatgctgttg
      301 aatcaattac tgatgaatcc aatacttcac taaatgagat gtccaagtat ttgaatacat
      361 ttaaggccga taattttgca tatgtttgat ctacggcttt agccgagtat aaactatcag
      421 gatcaatgta tgttttattg tataatgcag atggacgtat aaatttgatg tttgaatata
      481 caaatctatc tttgtactta attgattcca atgattgtaa tatagaatca tttaacaaat
      541 ctgttggttc gttaaccgta tagtaattaa tattacgtat cctatatgtc ttatgctctt
      601 cgctcttata tgtatctttt cctactacct tacgtagagg tcttactacc attaataaat
      661 caacttgatg tgtgtttaaa ctactatccg caataaatcc tatatactct ttatcaaaat
      721 aatagtatcc attgtttttt aagtattcgg ataagcgtcc tctttctgct tccaatgcgt
      781 ctgtatcaaa tagttcaccg gaatgtattg ttcgattaac cgaatcctta tatattaatt
      841 ctttaatttc aggattattt attctgtatt tgaagttcct tatacgatag ggctcatttg
      901 cttttattat gtactcaaca gaaacttttt tcttcttgaa cgtagcatta gaacttacaa
      961 atgcattttc gtatccttta ttggccaaat gtttttttag ttctttttca gatgtgcttg
     1021 tcaatgcggg atcgtatata acaggggcat caccgatttt ctgtaaaaat cggttaatcc
     1081 attgagtagt gtctcttcct gataagctat ataaggcaac attaaatgga ataaaatcaa
     1141 atagttttgt atttgggcgt tggcgcagat aagtaagcat atttactccc ttaagatctt
     1201 tcttatctgt tttaatcgat accttgttta ataaatattg accttccgga acgtatttag
     1261 tagtgctgca ggccgacaaa aatattgtca gtataaggca tgtatacgta aggacttttt
     1321 tagtgggcat attaaacaca atttcatgca aaagtaccac aaaaatacgg caatacaaat
     1381 atcttttgta acttcgcagt aataaataaa ctatatgtta agtaaaaata gaattaaata
     1441 tatcaaatct ttatctatta aaaagaatag atacgaagaa aaatgttttg ttgctgaggg
     1501 aagtaaaaca gtagaggatc ttttgtcttc ttttgtttgt cgtgcaatgt atggaacaga
     1561 aaggtatatt ggaattcgtc ccgattgttt tgaacttata acgccggcag aattaaaatc
     1621 tataagtaat ttggaaacac ctcaagatgt tttagctctg tttgaaatgc ctgaaaatat
     1681 atgtgattcg atggatgttt caggcaagtt gagtttggca ttagaaaaca tacaagatgc
     1741 cggtaacttg ggcaccataa ttagagtggc agattggttc ggtatagaga atattatgtg
     1801 ttctataggg actgttgatg tgtataattg caaaacggta caagcaacta tgggtgcttt
     1861 ggcacgtgta aatgtttact attgtgactt aaaaacctgt cttatgaagt tgtcttctga
     1921 gatgccaata tatgcaacat ctttagatgg agcggatata tatgcagccg aattgtcaaa
     1981 aaacggagtt gttgttatgg gaaacgaagg aaatggaata tcttacgaat taaaacaaat
     2041 atgtaattca aaattactga ttccaaatta tccgaaaggt aggaaaactt cagaatctct
     2101 caacgtagga attgcaacag ctgtcatttg cgccgaattc cgcaggccta gataatttgt
     2161 tataattcca attccgaaat ccattaattt gttggataga atctaaattt tgccagatac
     2221 ttttcggacg ctctttacta tagtatcttg acaaaataag ttgtttttct gcttccagat
     2281 tattgatgac tatattatat agttctttat actctttgtt gttaatgctg taccaagcaa
     2341 tacaactatc aaattgttca gggctgagtc cgtaattgtc tagtatttct ttgtagtaat
     2401 tatttaattt tcgcctgtct tgaatgtaat tgtatcttgt aagagcctca gttatatgaa
     2461 tttcagtcag tagtgaggcc attttatctt gctcaggcat aaaatcgggt ttattgacat
     2521 cttgtttgca agaacttaat ccaagtgcaa gcataactat tataatactt atgcatgatt
     2581 tcatttgaaa attctacgtt cgaatattag tagtaatgca actcctatac agatggccga
     2641 gtccgcaata ttaaaaacag gtgaaaagaa aataaaactt tcgcctccga agtatggtat
     2701 ccatgaccaa aatctgcctt caattaatgg aaagtacagc atatcaacta ctttgccgta
     2761 gaaaataggg gcatagccat aaatttgtcc ataaaaaatg cagtcaatta tattaccaaa
     2821 agctcctgca aaaattaaag ctaaacaaga taataataat aaactttcat ttttcttgat
     2881 acagtgaatt aaataccata gtattaatcc ggaagctact attctaaata aagtcaagta
     2941 tttcttatca aaaaaggaca taccaaaagc cattccttca ttctctaaga agtgtatgcg
     3001 aaaccacgag aaaatataca gctcttctcc taaatataaa tttgccttaa ccagatattt
     3061 agaaacttgg tctatgagta aaataagaaa tataataaag gcactaaaaa gtgccttttt
     3121 tgtctgattg ctcatttatt tcttttctgt tttagcttca atacttaaag tagcatgagg
     3181 aacagcacga agcctttctt taggaattaa ttttccggtt tcacggcata ttccgtatgt
     3241 tttgttttca atgcgtacca aagcagcttg caaatgttgt ataaatttca tttgtctttg
     3301 agcaagtctt cctgcttctt cttttgtcat ggtcgtagca ccttcttcca ataccttgaa
     3361 tgtgggtgat gtatcagaag tgtcattgcc atccaaatta tttagagcat tgcgcaattg
     3421 ttcataatca acgtaggctt gttgaagctt gtcattaatg ataacgcgga attcttctag
     3481 ttcagcgtct gaatatcttg ttttttctgc cataatatta gtttttagtt attatttttg
     3541 cgaatataaa gctattattt taaaaaacct aaaattacat tgcctttttt atatccaata
     3601 tgattttatt gtcatccatc tctattgcct gaccattggg caactcagaa acaatttcaa
     3661 tagaagtagc caatacttgt gatgcaatat aagatttgtg tttttcgatt gccgaatcaa
     3721 aatttttatc agattggatt ttgattttta tcttatctgt gatctctaat ccggtacttt
     3781 tacgcaagtt ttgtattctg ttaattgctt cacgagcaat accttcactt attaaatcat
     3841 cagtaagtgt aatatcaagt gcaatagtta atttgccctc atttgcgact aaccatcccg
     3901 gtatgtcttc tgagataatt tccacgtcat ctgtaagtat atagattcgt gtgtcattta
     3961 tagtatattc aaactgacct tcatcctcaa atttggtaat atctgtttga gacatatttt
     4021 gcaatagttg tgctacttgc ttcatgtctt taccacactt aggtcctaac tttttaaaat
     4081 caggcttaat gcgttttacc aagatacctg cagaattatc aaccaacttg atttctttta
     4141 catttacctc tgcttttatt agattttcaa cggcttttag gtttgtttct aattcggcat
     4201 tggttgaagg gatcattata tgactcagtg gctgacgtac cttgatattt gctttcttac
     4261 gtagtgctaa taccaaggat gttatgcgtt gtgcaacgtc catctgactc tccaatttac
     4321 tatctattaa agaagcatcg tatgttggga agtctgctaa gtgaactgat tctttagtgt
     4381 tcttatgagt tgcggcattt aagtcaagga atattctgtc tgcatagaat ggcgcaatag
     4441 gagccattaa acgagcaacg gtttctatac aggtgtataa agtttgatat gcggatagtt
     4501 tatccttatc catagaacct gcccaatagc gttttctgtt taatctgaca taccagttac
     4561 ttaggttttc tgtaacaaaa tcagatattg cgcgtccggc gcgagtaggt tcataatcgg
     4621 ccaaactttc gtctacttct ttaattagac tattaagtaa ggaaattatc caacgatcta
     4681 tttcaggtct ttcagataca ggaacatctt tttcgctata ggtaaaattg tcaacatttg
     4741 catataaagc aaagaacgaa taggtattgt atagggttcc aaagaatttc ctggagactt
     4801 cttcaacgcc atcagtatca aatttaagat tatcccatgg tgatgcattg gtcatcatat
     4861 accaacgtag aggatctgaa ccatatttat caatagtaaa aaatggatca acggcatttc
     4921 caagtctttt tgacattttg ttgccatttt tgtcaagtac taaaccactg gaaattacat
     4981 ttttgaatgc aacagaatgt tttaccattg tagatattgc atgtaaggta aagaaccatc
     5041 cacgagtttg atctacacct tcattgataa aatctgccgg gaatctggtt ccgttgtcta
     5101 tcaaatcttt gttttcaaat gggtagtgca attgtgcata aggcattgaa cccgaatcaa
     5161 accatacgtc aattaaatca agttctcttt tcatagcttt acctgatgct gatacaagaa
     5221 tgatgttatc tacgtaagga cgatgtaggt ctatgttttt gcagtcgtaa ttttcttttg
     5281 agaaatcgcc aactttgaaa tttttataag gattttcagt cataaaacca gccttaactg
     5341 atttttctat ttctgaaatt agttcagcta tacttcctat gcaaatttct tctttaccgt
     5401 cttctgttct ccatatgggg agtggagttc cccaataacg gctgcgtgat aagttccagt
     5461 cttgtaaatt ttccaaccat ttgccgaaac gtcctgatcc tgttgattga ggcttccatt
     5521 ttatagtctc gttcaattta aacatttcgt ctttgcgtgc agttgatttg ataaaccagc
     5581 tatctagtgg ataatacaat acaggcatat ctgttcgcca gcaatgaggg tagttgtgga
     5641 catgtttttc aatcttaaat gccaatcctt gttgctttaa catcatgcag attgaaatgt
     5701 ccaatgtttc gtctttatct gttaaagagg agtcgtatgc attttttaca taacgtcctg
     5761 caaattgttt gtaatcttca atattaacac gatcttttaa atatgttaaa tcgatgtctt
     5821 ctattctata aaacctgccc tgtaagtcta ctaatggacg ttgattacct aatttgtcga
     5881 taagaagcat agccggaatg cctgccattt ttgcaactct tgcatcatca gcaccgaatg
     5941 ttggcgcaat atgtacaata cctgtaccat cttctgtggt cacatagtct ccggcaataa
     6001 ctctaaatgc accttcgtca gctttaaaaa atggaatgag ttgttcatat tccataccaa
     6061 ctaaagttga acctttaaat gtctctgaag gtttttcttg acccttaaaa taactgtcaa
     6121 ataagtctgc tgcaagtata agctctacat cttcttttgt ataagggttt tgtgtggcgt
     6181 atctcacata atctatgttt ggacctacgc aaagtgccgt atttgaagtt aatgtccatg
     6241 gtgttgttgt ccatgctaag aaataaagat tcttttcgcc tttaacttta aattgtgcgg
     6301 taacagtagt atcttttacg tctttgtagc acccgggttg attaagttcg tgtgaactta
     6361 atccgctacc ggctgcagga gagtatggct gaatagtgta tcctttgtat agaagccctt
     6421 gcttatataa ttgttgtaat aaccaccaaa tactctctat gtagctattt tcgtaagtga
     6481 tatatggatt atttgtgtca acccaatatc ccattttatg agttaaactt tcccattctt
     6541 tggtatattt cattacgtct ttgcgacata ttgaattata ttcttctaca gaaatcgttt
     6601 tgcctatatc ttcttttgta attccaagag atttttcaac gcctagttca acaggaagtc
     6661 cgtgtgtatc ccatcctgct ttacgttcta ccatgaaacc cttcatggtc ttgtatctta
     6721 aaaacaagtc tttaatggta cgtgccatta cgtgatgaat gccaggcata ccattagctg
     6781 atggcggtcc ttcatagaac acaaatgaag gatgaccctt gcgtgtgtcg atactttttt
     6841 gaaagatatt gttcttgtcc cactgctcca aaatctcttt gttgatttca gacagtttaa
     6901 taccttgata ttcggcaaaa tgcttattca tactacactt tttatataaa acgtgcaaag
     6961 atacaaaaaa gcaagtaata aaaaaagatg gtgtttacct aaaactatgt tagtttaagt
     7021 cgctttttaa cgcagtcttt ccattgagtg tacaagagct tcgtctttgc ctatttctct
     7081 tatagcaagg tagctaagta taatgttaat tggaagaata acaattggcc aagaaatttt
     7141 aattgatgca tctgcattat tgtttttgta caataagtaa atacataaaa cgattagcaa
     7201 tagttggaat accattaata tgatattgaa acttgtaaag cgtatttgta gtatgcgctt
     7261 cttatatata aaaattgtta tcagcgaaat tagtggaata agtatacata atactgagta
     7321 tggcataaaa ctaccaaatc caactgtatg ttcggctaca tgggcaacag gaaaaaaaca
     7381 catcaacgtt ccgagtacaa atgctatgaa taaatatacg gtttgtatgc gctgtatcat
     7441 tgtatttatg atatttaaat gtgaaagaaa agaaattttt acggattcat gttgttaacg
     7501 taaaattaaa aaaaaatccg tgaggctttg cctcgcggat tttgtgcttc tttttgctaa
     7561 aaattattca gcaacaacag cagcagaatc agcaactaca gcagcagcag aatcaacaat
     7621 aacttcttct acaggtgcaa caacttcttc tacaacagct tcttcagctt cttcttgagc
     7681 agcctctttg ttacaagatg caaatgcaac agcaacgatg gcagcagcga acaacactaa
     7741 ttttttcatt tttgtaattt taaaaattaa acaatgattg ttttcttatt tcgcaacaaa
     7801 ggtattcatt atttcagtgt agttattact ctaatactaa atttatttgt tatttaatgt
     7861 taaaacgatg ttttgtagca ttgttttgat gttaaatatt caaatttgta taaagataac
     7921 gtttgatact gcgttttggt gtcttgtcga actcattcgg atataaaatt atcttagaaa
     7981 tattctcgta atttgccacc atactattaa gttgcttgcg attttcttcc ataataatat
     8041 ctaagtcgga atgtgagata ttagatgcat caactgcttc ataatcagga tatactaagc
     8101 cgagtaattt tccgtctttg tcaattacaa ggctctccat aacaaatgga aggttattta
     8161 atttttcttc aatttcttcc ggatatatat tttgaccgct tgcacctaat atcatgcttt
     8221 tgcatcttcc ttttataaac aggttgcctt ctttgtcaat ggtacccata tctccggttc
     8281 tcatccatcc gtcttctttg aatatatttt ttgtggcttc tacattctta tagtaaccgc
     8341 tcatcacgtt ttcgcctcta acgcatattt cgccgactat attgtatgga tctttagaat
     8401 caatcataat ctccattata tcaagaattt taccagccga atatggaaca aatttgtcgt
     8461 gtggtgaata gcatataaga ggagcgcatt ctgtcatacc gtagccgaca gtaaatttaa
     8521 agccgatttt tcttaaaaaa tcctcaactt ctttattaag aggtgcacct ccaataataa
     8581 cttctctgaa ttcatttcca aatgcttcat taagtttctt tttgatttgt tgatatatct
     8641 tgtcatctaa caatggaata cttagtgcta gtctcatact cattttgttc aatgcaggtt
     8701 gaagttgttt cctgtatatt ttttccatta taagcggaac agtaaatatg actttaggtt
     8761 ttatttcttc aaaagccttc atcaatactt taggactcgg aatcttgttt agtagataag
     8821 tgtgtgaacc tacgcatgta gctgttaaaa agtcgaatgc cataccgtat gcatgtgcca
     8881 gaggtaaaaa tgcaagtact ttatctcctc tttctagtag gtgtgtcctt attccgaatg
     8941 tgatatttcc tgcaagtgca ttcccgctaa tcattacgcc tttactaaaa cctgtagtgc
     9001 ccgaagtgta gctgatcatt gccaattctg cattatcgcg ctctgtatat ttaacgtgtt
     9061 cctgtttata gccttgtgga tagcgtttgt tgaaattgtc tgttaatgaa ttcatgattt
     9121 tctgaatgta ttctccatct ttttggtgaa tgcatctgaa atcgttaagt gaaaaaacgg
     9181 ctataagatt attcattttt tcctcttcaa gtgtatccca tattttgtcg ctacagaata
     9241 gcattttaga ttctgagtga ttaacaatat ggtgtacatc gtttgggttg aaatcttgca
     9301 agattggtac tattactgct ccgtatgtta caacagctat gtatgtaatg gtccaattag
     9361 gtgtatttct gccaattaga gcaattttat cgccttgctc aacttttgca ctttcaaata
     9421 acaagtggag ttttgcaatt tgatttgcgg cctcgctgta tttgaaggtt tgattcttgt
     9481 agtagttagt gtaaattgga taatcccagt tttctttaaa actgtgttca aatagcttaa
     9541 taaaattttg ttctatcatt tttgcacact tttgattaat ttgtgcaaaa atagaaacaa
     9601 tttattgatt ggacaaaaaa atatctttga attgcttgtt tttatgtctg aagatatagt
     9661 aaaaaactat ttattgtgca gaaattttat gttatttcag atatatttat ttgatttgca
     9721 atattttaca ataaaaatta taaaaacgat gaggatacac tataaaatta acaattcaat
     9781 gtttgttgat tcggcaaatg ccttaaatct aataactaag tgttgggtag tgggtgggtg
     9841 aagaaaatta ttccaaacca ttcaattctt ttagccaaat gtttgctgag gcatcgctgg
     9901 gcatgcgtaa atcaccacga ggagataatg caatagaacc tattttaggg ccatcgggaa
     9961 ggcagttacg tttaaattgc tgactaaaga aacgcttata gcaaatctta agccatttta
    10021 aaatagtagc cttttcgtaa agaacatcgt cagaaacttc tttttcagaa aaagctgttt
    10081 cagccaacgc cattattttt gacggagaaa acccaaaacg cagcatataa taaataaaga
    10141 agtcatgtaa ttcgtacggt ccgacaacat cttcagtttt ttgcacaatc tcgtcattat
    10201 cgcctttggg caataactca gggctaatag gcgtatcaat aatatcagcc aagatagcct
    10261 ttgttttagt atcgtgcagc gtagcgtatg catttaccaa actgcgaacc aatgttttag
    10321 gtacacctac attaacacca taatgagaaa tatggtcacc attgtatgta caccatccaa
    10381 gagcaagctc actcaaatct ccggtaccaa ttacaatgcc gttagtcttg tttgccatat
    10441 ccatcaatat ctgtgtacgt tcgcgagctt gtgtgttttc atagcagata tcatggactg
    10501 aaatatcgtg attaatatct ttaaaatgtt ggatgcaagc gtctttaata gaaatagtga
    10561 ttgaagttgt atttaaagac tccatcaatt ttatagcatt acccatagtt cttgatgtag
    10621 ttccaaatcc tggcatggtt acacccatta tattttttcg gtcgtaacca agttcgtcaa
    10681 aagcttttgt tgctacaagc aaggcaagcg tagaatcaag tcctccggag attcctataa
    10741 taacgttgtt acaatttata tgttccaacc ttttctttaa gcctgccact tggatatcca
    10801 taatttcaga gcatctttca tctttttccg gtccgttatt aggaacaaaa ggtctgcgct
    10861 caattggata tacaatttgg tcaggtttta aatcaatatt agatgatatg tatctaacag
    10921 gcgtataata cgatgatttt gcatcagcat aactactctg tttcatacgg tcatatctta
    10981 aacgctcaat atcaatatct gaacatatta gatcttgatc acatataaat ccgttttctg
    11041 ataaaatacg accat
//
LOCUS       DAOPID010000025        11005 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_7237_length_11005_cov_4.654064, whole genome shotgun sequence.
ACCESSION   DAOPID010000025 DAOPID010000000
VERSION     DAOPID010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 11005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11005
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7237_length_11005_cov_4.654064"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2280)
                     /locus_tag="PK641_01815"
     CDS             complement(<1..2280)
                     /locus_tag="PK641_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HPG55125.1"
                     /translation="MKKKLIFLLTLFLSTAQFAGAECILLNASSEFSLGLGGSKEFTF
                     SPGNTPTLSYEAYKTATTAIGSFYVYQYVNGGWVEVQSIGAGSLAKNSYKSFSVQLNS
                     SATKIKFEFPWGGSYKRYFRNIKVTQASYFNVSPASIDFGAVEIGKTTTKTINVVYSS
                     QSGSVSSSNVGVFSVDKTSVGDSNCGYGNTTVQVTFKPISTGSISATINVGGNNVTVT
                     GSGSLAVPSGLTASNVSYTSANFSWNAVSGASAYLLTNTTNSKTYRVRATSTTISDLV
                     PNNAYTFNVKSLDASDNASAASTPVSITTKTIGVPANLQVSDIGYSSVNLSWNAVNDA
                     TGYRLTNLTTGDEYDVQGTSCEATGLGLNTTYSFAVKSLYNTYESAESTPVSATTLNI
                     EADLTACTVLNDASQYQLMDGWSTGTPKEFLLSGPGKKMYFTAVFGAANTGTIYAEEY
                     YADGTYKKLITVNAILWGGGQTNAVCDISSQAVKIKFVATGTLVKSVKDVRVEQAYSF
                     SVSSTTLDFGTVTIGKSATRTLNINYSNAADMSAVFDNEYFSTTTDLPKACAHGSQTI
                     TISYEPKVLDTPQASSLVLFNGISHTIVVRGETTPLPAPTNFRVAQASAHSIPLLWDA
                     VEGATAYRLINTTTNDSIDVTETSYTFDDLNAHSDYAFTVSSLYNNEVCGTSSVSTKT
                     YTPLYIGVLGNAAGCISSSVTLVSGNMNIEDNSYKVGSEIALNVNSNNESVEIEITAN
                     GETIQSPNSILLKGGDIVSI"
     gene            complement(2307..2612)
                     /locus_tag="PK641_01820"
     CDS             complement(2307..2612)
                     /locus_tag="PK641_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HPG55126.1"
                     /translation="MIRHLVMWKLKANAEGKTKAENAHLIKQNLENLSGLIPCLKFIH
                     VGINLDASSPSNYDVVLDTSFENLADLDAYQVHEAHVRVAGYIKRIVDDRICVDYEF"
     gene            2984..3973
                     /locus_tag="PK641_01825"
     CDS             2984..3973
                     /locus_tag="PK641_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPG55127.1"
                     /translation="MSAGNCAIVKPSELSSATSKLIGDIISKNFEQSYIAVAEGGKEI
                     SEALLSEQFDYIFFTGGTSIGRIVMQKAAENLTPVTLELGGKSPCIIDKSADIELAVK
                     RILFGKIINSGQTCVAPDYLLVHADIKDKIKDAFVKYSTKMLGKNALENKSYPRIINR
                     RHFDRLVAYLQDGEIISGGEYRTDTLQISPTLLSVNGLSSKVMEEEIFGPILPIITYS
                     STNEAINIINSRPKPLALYLFTTNSSVDSNVIKNVSFGGGCINDTIIHLASPKLPFGG
                     VGSSGMGSYHGKYGFDTFSHHKSILKKANWLDLPFRYPPFSKIKLQLIKLFLG"
     gene            complement(4001..5359)
                     /locus_tag="PK641_01830"
     CDS             complement(4001..5359)
                     /locus_tag="PK641_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPG55128.1"
                     /translation="MTFEDYLPYYKRNLRIAGPVVLSQLGGSITQLADNIMVGHLGTV
                     ELAAVSFANTIFIIGMLFSIGITMASTPLIGQAYVQGEHTKLTSYLQNSLLQVILTAI
                     VISALLFALYPFMDDMGQEPDVVKLAKPYYILLVISLFPFLIFCFFKQFLEGLGSTKH
                     AMIITIICNIVNIVLNYFLIFGTYGFPKWGIIGAGVATLLSRVLMPIMFLIIIRKKTD
                     WWMFFNRFGKKVFSFKVLRELHSLGLPIAGQMVLEISAFAFSAIMVGWLGAVPLASHQ
                     IAQNISTIAFMVVLGISTATTIRVSHQLGLRDFKALRMAASASIHLCLATNFILGLLI
                     LSFRYIIPIAFTEDAAVIELAAQLLLFVGLFQLFDGMQSVGIGILRGLTDVKRPVIYA
                     FISYICINLPIGYIFAFVFKMGAAGIWLGFVFGLGVAAILFHIRYRKRINELESAYYK
                     TC"
     gene            5426..6616
                     /gene="purT"
                     /locus_tag="PK641_01835"
     CDS             5426..6616
                     /gene="purT"
                     /locus_tag="PK641_01835"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HPG55129.1"
                     /translation="MLELGTTLSPIGKKALLCGSGELGKEVAIELQRYGIEVIAVDRY
                     KNAPAMQIAHRSHVISMLDGNALRSVIEQEKPDYIIPEVEAIATKTLVELEKEGYNVI
                     PTANAAFLTMNREGIRKLAAEQLGLPTSKYLFADTYEEFNTAIKEIGIPCVVKPIMSS
                     SGHGQSVIKKAQDIDIAWHNAQEGGRSGAGRVIVEGFVPFDYEITLLTVRHSGGTSFL
                     QPIGHHQVDGDYRESWQPQAMSEKCLNQAREIAKKITDALGGWGIFGVELFICKDKVL
                     FSEVSPRPHDTGMVTMISQDLSEFSLHARAVLGLPIPSIRFYRPSASKAIVVEGDTDK
                     VIFSGLEDVLNEEDVQIRIFGKPEVKGHRRLGVILASANTVDEAKAKAERAYKKLKVK
                     ILAR"
     gene            complement(6688..8997)
                     /gene="topA"
                     /locus_tag="PK641_01840"
     CDS             complement(6688..8997)
                     /gene="topA"
                     /locus_tag="PK641_01840"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPG55130.1"
                     /translation="MGKNLVIVESPAKAKTIEKFLGKDYHVTSSFGHIRDLKTKDISI
                     DFKKNYHPIYEVPADKKKLVADLKKKVKESEAVWLASDEDREGEAIAWHLAEELKLDT
                     KTTKRIVFHEITKNAILEAIEHPRNIDLALVDAQQARRVLDRIVGFELSPILWRKIKP
                     ALSAGRVQSVAVRLIVEREREIQQFRPEASYRVTAIFKTQLGEEIRAELGNRFKNKAT
                     AESFLEKCKNASFTIEDITTKPSKRTPAPPFTTSTLQQEASRKLGYSVSQTMRIAQRL
                     YEDGKITYMRTDSVNLSSLAINAGKQEILAFAGEKYSKVRQYTTNSKGAQEAHEAIRP
                     TYMSAHTIEGTKQEQYLYELIWKRTIASQMADAELEKTSVIIGISDSEDKFIASGEVL
                     KFDGFLKVYMESKDEEEEDGDMRILPSVKIKDELIYKKIACTQRFTQRPPRYTEASLV
                     RKMEELGIGRPSTYAPTISTIQNREYVIKSDKEGNKREYNELILTKGLIKDEILSEVV
                     GTEKNKLFPTDTGTVVNDYLIKNFGNIVDYNFTAKVEKDLDKVAEGKTQWEKTIDIFY
                     KGFHPLIDASLQEHTDHKVGERILGKDPKSGRQISVKIGRFGAVAQLGTASDTDKPVF
                     ASLQKGQSIETVSLEDVLELFKLPRLVGNFEGKEIKANTGRFGPYVMHDSKFFSLTKD
                     VDPMSITEEQAIELIKAKREAETNKVFKSFDEDKSMQLLNGRYGIYLCKDKQNYKLPK
                     DKKAEELTYADCLKIIEAAPAKETKRRKK"
     gene            9333..>11005
                     /locus_tag="PK641_01845"
     CDS             9333..>11005
                     /locus_tag="PK641_01845"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG55131.1"
                     /translation="MKYISRFVVLALMCASLTPCIAQRSPFDNTQHRLFSEGQSFFTE
                     KNYSASTRYLEEYLKTNPDAESEELRQAKYYIAMSSYILRKDDAQSKLEAYQARYPYS
                     TDNERIHLYLGILEFESGKYKPAIKHFEQIKAAELDEEEFLELLFYKGYAYVKQESYE
                     KAAYELQELLKKPENKFSQAARYYYGFSQYRLENYETAYEYLKSVEDHPDFQTTTPYI
                     ICQCLYKMDRCSELQSYGKALLDKGGNNKNINDIKHLLGACSFKSQNYADALEYLSLY
                     KTATKKISREDWYMLGISAYKTDDFKNAIAYLSKATSKEDILTQNSYFHIAMAYIKLG
                     DKKNARMAFEAAARYKYDSGIQEDALYNYALVTYEMSFSPFNESVVAFERFLKYFPNS
                     QYVPKVYEYLLNVYLTTKNYDAAYQSIQNIKSDNPAIKEAEQRVLFGMGTTAVANRKY
                     GAAAKNFETIINNRSYNYEITARSYFWYGECMYKMKKYDEAKKAYEGYLNKIGSKNGD
                     EYSLAHYNLGYVYIKQNDYKEANLWFRKFINLESEDKVMLVDACNRIGD"
BASE COUNT         3644 a         2020 c         1913 g         3428 t
ORIGIN      
        1 aattgaaact atatcgcctc ctttaagtag aatgctgttt ggcgattgta ttgtttcacc
       61 gtttgcagtg atttctattt ctacagattc gttgtttgag ttaacattaa gtgcaatttc
      121 agaacctacc ttatacgaat tgtcttcgat attcatattt ccgcttacaa gtgtaacaga
      181 cgatgagatg caacctgcag cattgcctaa tactcctata tataaaggag tgtaagtttt
      241 tgtcgaaaca cttgatgttc cacaaacttc attattatac aaagaactta ctgtaaaggc
      301 ataatcactg tgtgcattca aatcatcaaa ggtatatgac gtttcggtaa cgtcaatgct
      361 gtcgttggtt gttgtattga taagacgata agcggtggct ccttccacag catcccaaag
      421 caaaggaatt gaatgtgccg aagcttgtgc tactctaaaa ttagtaggtg caggtagcgg
      481 agtggtttca ccacgtacta ctattgtatg agagatgcca ttgaacaaga ctaaacttga
      541 tgcttgtgga gtatccaaca ctttgggctc atagcttatt gtaatagttt gtgaaccatg
      601 ggcgcaggcc tttggcaaat cggttgtagt gctaaagtat tcgttatcga atactgcact
      661 catgtcggcg gcgttggaat aattaatatt taaggtgcga gtagccgatt tacctatggt
      721 tacagtgcca aaatcgagag tagttgaact tacagaaaag ctatatgctt gttctacacg
      781 cacatcctta acggatttaa caagtgtacc tgttgccaca aatttaatct ttaccgcttg
      841 ggaactaata tcacaaactg cattggtttg accccctccc cataaaatag cattaacggt
      901 aattaatttt ttgtaagttc catcagcata gtattcttct gcataaatgg tacctgtatt
      961 ggctgctcca aaaacagcgg taaaatacat ctttttgccg ggtcccgata acaaaaattc
     1021 ttttggagtc cctgtcgacc agccatccat caattggtat tgactggcat cgttcaacac
     1081 agtgcaagca gtaagatcag cttcaatatt taaggtagtt gcagacacgg gtgtagattc
     1141 ggcactttcg tatgtgttgt acagcgattt aactgcgaat gaataggttg tattcaatcc
     1201 caagccggtg gcttcgcacg aagtgccttg cacatcatat tcgtcaccgg tggttaggtt
     1261 ggttaagcga tagccggttg cgtcatttac cgcattccac gaaagattta cagagctata
     1321 acctatatcg ctaacttgta ggtttgctgg aactcctatt gttttagttg taatactaac
     1381 tggagttgat gcagcagaag cattgtcact tgcatcaaga gattttacat taaatgtata
     1441 tgcattgttt ggtactaaat ctgagatcgt agttgaagta gctctcaccc tataggtttt
     1501 actatttgtt gtattagtaa gaagatatgc gcttgcacca ctaactgcat tccatgaaaa
     1561 attagcagat gtatatgata cattacttgc cgtcaaaccg cttggtacag ctaaagaacc
     1621 tgaacctgta actgttacat tattaccacc tacattaatt gtagcagaaa tagaaccagt
     1681 agaaataggc ttaaatgtta cttgcactgt tgtattaccg tatccgcaat tactatcccc
     1741 taccgaagtt ttatcaacgg agaagactcc cacattgctg gaacttacag aaccggattg
     1801 tgatgaataa actacattaa ttgtttttgt tgttgtttta cctatttcta cagctccaaa
     1861 atcaatactt gctggtgata cattaaaata cgatgcttga gtaactttga tatttctaaa
     1921 atatcttttg taagatcctc cccatggaaa ttcaaattta atcttagttg cactactgtt
     1981 taattgaact gaaaatgatt tatagctgtt ttttgctaaa cttccagcgc ctatagactg
     2041 aacctctacc catccaccat ttacatattg atacacataa aaactaccta ttgcagtcgt
     2101 cgcggtttta tatgcctcat atgacaaagt aggcgtattc ccaggactaa aggtaaattc
     2161 ttttgaaccg cctaggccaa gactgaattc agatgacgca ttaagcaaaa tgcattctgc
     2221 tccggcaaat tgtgcagtac ttaaaaatag agttaataaa aatattaatt tctttttcat
     2281 atattttctt atttgtgagt ttttttttaa aattcgtaat caacacatat tctatcatca
     2341 actatacgtt taatatatcc tgcaactctt acatgagctt catgcacttg atatgcatcc
     2401 aagtctgcta aattttcaaa acttgtatca agaacaacgt cataatttga tggtgaggag
     2461 gcatctagat tgatacctac atgaatgaat tttagacaag gtatcaaacc cgacagattt
     2521 tcgaggtttt gcttaatgag gtgcgcattt tccgcttttg tcttaccctc agcatttgct
     2581 ttgagtttcc acataacgag atgtcttatc ataaaaatct ttttttacgc gacaaagata
     2641 tataaaaaat gtgttctgca gcatatctta gatatgaaaa ttaatgttgc aaaacataat
     2701 ttttaccgcg tatcccatta aatggcagtt ttatttattg tgttaaatgt taaaatcaat
     2761 aatatttaca catctttttc ggcaatattt tagaaaatca caaattttgt actgcttttt
     2821 tactcaaaaa gggatatgga gcaataaata ttaataaatg tttataaaaa acagaagagc
     2881 gatgaatttt ataattgcgt attccataaa aaatgccgaa tattgcacta ttagttatta
     2941 aagcaatgaa aaataaataa aaatagaccc actcatcgca gctatatcag caggaaattg
     3001 cgcaattgtt aaaccatcag aattatcatc tgccacatct aaacttatag gagatattat
     3061 aagcaagaac tttgaacaat catatatagc agttgcagaa ggaggtaaag aaattagcga
     3121 agccctattg tcagaacaat ttgattacat attttttaca ggaggcactt ctataggacg
     3181 aatagtgatg caaaaagctg cagaaaactt gactcccgtt acacttgaat taggaggaaa
     3241 aagcccttgc attattgaca aaagcgcaga tatagaactt gcagtaaaac gcattttatt
     3301 tggtaaaata attaattcgg gacaaacctg tgttgcaccc gattacttgc ttgtgcatgc
     3361 cgatataaaa gacaaaatta aagatgcatt cgttaaatac agcacaaaga tgctaggaaa
     3421 aaatgcatta gaaaacaaat catatccgcg tataattaat aggcgtcatt ttgacagatt
     3481 agttgcatat ttacaagatg gtgaaattat atcaggaggt gaatatcgta cagacacact
     3541 tcaaataagc cctaccttac tatctgtaaa tggtttaagt agtaaagtta tggaagaaga
     3601 aatttttggt ccaatacttc ctataatcac atacagcagc acaaacgaag ctatcaatat
     3661 aatcaattcc agacccaagc cattggcatt atatttattt acaacaaact caagtgtaga
     3721 ttctaatgtt ataaaaaatg tatcatttgg aggaggatgc attaatgata ctatcataca
     3781 tcttgcatcg cctaaactac catttggagg agttggaagt agtggtatgg gctcatatca
     3841 cgggaaatac ggatttgaca ccttcagcca tcacaaaagc atcttaaaaa aagctaattg
     3901 gctagattta ccgttcagat atcccccatt cagcaaaatc aaattgcaac taataaaatt
     3961 attcttggga taaaaaaaca aaaaatcatt gagataagaa tcaacatgtt ttataatatg
     4021 ctgactctaa ctcgtttata cgtttcctgt atctgatatg aaataaaatt gcagctactc
     4081 ctaatccgaa tacgaaccct aaccaaatgc ctgcagcccc cattttaaag acaaatgcaa
     4141 atatatatcc tattggaaga ttaatacaga tatatgatat gaaagcatat attacagggc
     4201 gttttacatc ggtcaagcct cttaagattc ctattcctac agattgcatt ccatcaaaca
     4261 attgaaacaa gccgacaaat aataataact gtgctgcaag ttcaataact gctgcgtctt
     4321 cggtaaatgc gattggtatt atatatctga aactcaatat taataatccc agaataaaat
     4381 tagttgctaa acatagatgg attgaagctg atgcagccat ccgcaatgct ttaaaatcgc
     4441 gtaaacccaa ttgatgacta actctaatgg ttgttgcagt agatatgcct aaaactacca
     4501 taaacgctat ggttgaaata ttttgagcaa tttgatgtga tgccaaaggg acagccccaa
     4561 gccaacctac cattatcgca gaaaaagcaa atgcagatat ttcaagtacc atttgacccg
     4621 caataggcaa accaagcgag tgcaattctc ttaatacttt aaacgaaaat acttttttgc
     4681 cgaatcgatt aaaaaacatc caccaatctg tttttttacg tataataatc agaaacatta
     4741 ttggcatcag cactcttgaa agtaatgttg caacaccggc tcctattatt ccccacttag
     4801 ggaatccgta agtaccgaat atcaagaaat aatttaatac gatatttaca atattgcaaa
     4861 ttattgtaat aatcatagca tgtttagtgc ttccaagacc ttcgagaaat tgcttaaaga
     4921 aacagaaaat caaaaatggg aataaactta tcactaaaag tatatagtaa ggcttagcta
     4981 atttcactac atcaggttct tgccccatgt catccataaa tggatatagc gcgaataata
     5041 atgctgaaat tactatagcg gtcaaaatca cttgtaacaa cgaattttga agatatgacg
     5101 taagttttgt atgctcgcct tgaacgtatg cctgacctat tagtggtgtt gatgccatag
     5161 ttatcccgat tgagaaaagc atcccaataa taaaaattgt atttgcaaaa gaaacggctg
     5221 ctaattcgac cgtaccgaga tgcccaacca taatgttatc agcaagttgc gtaatagaac
     5281 ctccaagttg cgaaagtact accggtccgg caatcctcaa atttctttta taatatggta
     5341 aataatcttc aaaagtcatg ggcacaaagg tagttctatt tgagagaaaa ataataactt
     5401 tacagccatt ataaaatcaa aaaatatgtt ggaattaggt actacattat ctcctatcgg
     5461 caaaaaagct ttactttgcg gctcgggaga attaggaaag gaagttgcaa ttgaattaca
     5521 acgatatggc attgaggtaa tagctgttga tagatacaag aatgctcctg ctatgcaaat
     5581 cgcacataga tcgcatgtga tttcaatgtt agacggcaat gcactgcgct cggttattga
     5641 gcaagaaaaa cctgattaca taattccgga ggtcgaagcc atagctacta aaacccttgt
     5701 tgaactcgaa aaagaaggat ataatgtaat tcctacagcg aatgcagctt tcttaaccat
     5761 gaatcgagaa ggtattcgca aactagctgc agaacaatta ggtttaccaa catcaaaata
     5821 tctttttgcc gatacatacg aagaatttaa cactgcaatt aaagagatcg gtattccatg
     5881 tgttgtaaaa ccaattatga gttcttccgg gcatggtcaa agtgttataa aaaaagcaca
     5941 agatattgac atagcatggc ataatgcaca agaaggagga cgctcaggag caggtagagt
     6001 tattgtagag ggttttgtac ctttcgatta tgaaataacc ttactgacgg ttaggcattc
     6061 gggcggaaca agctttttgc aaccgatagg acatcatcaa gttgatggtg attacagaga
     6121 atcatggcaa cctcaagcta tgtcggaaaa gtgcttaaac caagcaagag agattgcgaa
     6181 gaagattaca gatgcattgg gaggatgggg tattttcggt gttgaattat ttatttgtaa
     6241 ggataaggtt ttatttagcg aagtttcacc aagacctcat gatacgggaa tggtaacaat
     6301 gatatctcaa gatttatcag aattttctct acatgcacgt gcagtactcg gacttcctat
     6361 tccaagcata cgcttttatc gtccatctgc atcaaaagcg attgttgttg agggagatac
     6421 ggacaaagtt atattctccg gtcttgaaga tgtacttaac gaagaagatg tacaaataag
     6481 aatctttggc aaacctgaag taaaagggca tcgccgactt ggtgttattt tagcatcagc
     6541 aaatacagta gacgaagcca aagcaaaggc tgaaagagca tacaaaaagc taaaagtaaa
     6601 aattttagca agataattat atatcaaaaa cggctataca ttgagattta actcatagta
     6661 tagccgtttt tttaatctat ctgaaattta tttttttctt ctcttagtct cctttgcagg
     6721 tgcagcttca attatcttca aacaatctgc ataagtcaat tcttcagctt ttttatcctt
     6781 aggcaacttg taattttgtt tgtctttgca caaataaatt ccgtaacggc catttaataa
     6841 ttgcatagat ttatcctcat caaacgactt gaaaacttta ttagtttctg cttcgcgctt
     6901 agctttgatt aattcaatag cctgttcttc tgtaatactc attggatcga catccttggt
     6961 cagcgagaag aatttgctgt cgtgcataac atagggaccg aaacgacctg tattagcctt
     7021 tatttctttc ccttcaaaat ttcctactaa acgtggaagt ttaaaaagtt caagtacatc
     7081 ctctaaactt acagtttcta tagattgtcc tttttgcagc gatgcaaaaa caggtttatc
     7141 ggtatctgaa gccgtgccta actgagcaac cgcaccaaaa cgacctatct taaccgatat
     7201 ttgtctgccc gatttaggat ctttacctaa aatcctctct ccgactttat gatcggtatg
     7261 ctcttgcaaa gatgcatcaa tcaatgggtg aaatccttta tagaagatat ctatagtctt
     7321 ttcccactgt gttttaccct ctgcaacctt atctaagtct ttttctactt ttgcagtaaa
     7381 attgtaatcc acaatattgc caaagttctt tatcaaataa tcatttacta cagtacctgt
     7441 atctgtcggg aacaatttgt ttttctcggt tccgactact tcactcaaga tttcatcttt
     7501 tataagcccc tttgtaagaa tcaattcgtt gtattcacgc ttatttcctt ctttgtcaga
     7561 ctttataaca tactctctat tttgaattgt acttattgta ggagcataag ttgaaggacg
     7621 tccgattcct agttcttcca tttttctaac taatgatgct tcggtataac gtggcggacg
     7681 ctgtgtaaaa cgctgtgtgc aagcaatttt cttatatatt aattcgtctt ttattttaac
     7741 cgaaggcaaa atacgcatat caccgtcttc ttcttcttca tccttcgact ccatataaac
     7801 tttcaagaaa ccatcaaact tcaacacttc gcctgatgca ataaatttgt cttcggaatc
     7861 agaaattcct ataattacag aggttttttc aagttcagca tcagccattt gtgatgctat
     7921 tgtccttttc caaatcaact catacaaata ctgctcttgt ttcgttcctt caatagtgtg
     7981 tgccgacata tatgtaggac gaattgcctc gtgagcctct tgagcacctt tcgagtttgt
     8041 agtatattga cgaaccttac tgtatttttc gcctgcaaat gccaagattt cttgtttacc
     8101 ggcattaatt gccagcgaag ataggtttac ggagtcggtt ctcatgtatg ttatcttacc
     8161 atcttcgtac agacgctgag caattcgcat tgtttgagaa actgaatatc ctaatttgcg
     8221 tgatgcctct tgttgtaagg ttgaagttgt aaatggtgga gcaggagtac gttttgaagg
     8281 ttttgtagta atatcttcaa tagtaaaact tgcattttta catttttcta agaatgattc
     8341 agctgtagcc ttattcttaa atcgatttcc taattctgcg cgaatttcct cacccaattg
     8401 tgttttaaat attgcagtta cacgataaga tgcttccgga cgaaattgtt gaatttcgcg
     8461 ttcgcgttca acaattaaac gaacagcaac agattgtaca cgtcctgcag aaagagccgg
     8521 tttaatctta cgccataaaa taggtgatag ttcaaaacct actatgcggt cgagtactct
     8581 acgagcttgt tgagcatcaa ccaaagctaa atctatatta cgtggatgct caatagcttc
     8641 taatatcgca ttctttgtaa tttcgtgaaa aacaattcgt ttggttgttt ttgtatctaa
     8701 tttcaactct tcggccaagt gccatgcaat tgcttctcct tcgcggtcct catcggatgc
     8761 caaccataca gcctcacttt cttttacctt tttttttaaa tctgcaacca atttcttttt
     8821 atcagcagga acctcataaa taggatgata gtttttctta aagtcaatac tgatgtcttt
     8881 cgtttttaaa tcacgaatat gaccaaaact cgaagtaaca tgataatctt tgcctaaaaa
     8941 tttttctatg gtcttagctt tggcaggaga ctcaacaatg actaaatttt tacccatgag
     9001 cgtaatattt tgagaaagtg gatggaaatt cggtgcaaaa gtaataaaat ttatttcgtt
     9061 gcaagcaaaa tatataagaa taaggtgtaa tactcaatta tctgaaattt aaaataagaa
     9121 gtgtgaaacg caaaaagcat aaaaaaattg taatttcgca cttgctactt gaatatatac
     9181 ttcggcaaca tgcacaactt tttaaatact caacaaaaat ggaacaataa attaattatc
     9241 tttgcggtcg ttataataac actattaggt tttaagacaa taaattgcat caatattcgt
     9301 ttaaatatta tgcactaaaa tcatattttg taatgaaata catcagccgt ttcgttgttt
     9361 tggctcttat gtgcgctagt cttactccat gtattgcaca aagatcacca ttcgataaca
     9421 cacaacatcg cctcttttcc gaagggcaat catttttcac cgaaaaaaat tactctgcat
     9481 ctacacgata tttagaagag tatttaaaaa ctaatccgga tgccgaatca gaagaactta
     9541 gacaagctaa atattatatt gcgatgtctt cgtatatact tcgaaaggat gatgcacaat
     9601 caaaattaga agcatatcaa gccaggtatc catactctac tgataatgaa cgtatccatc
     9661 tatacctcgg cattcttgag tttgagtcag gcaaatacaa accggcgatt aaacattttg
     9721 aacaaataaa ggctgcagaa ttagatgagg aagaattttt ggaattgcta ttttataaag
     9781 gatatgcata cgtaaaacaa gagagttacg aaaaagcagc atacgaatta caagaattac
     9841 ttaaaaaacc ggaaaacaaa ttcagtcaag ctgcaagata ttattatgga ttttcgcaat
     9901 atcgtttgga aaattatgaa acagcatacg aatatctaaa atctgttgaa gatcatcctg
     9961 atttccaaac gacaacacca tatataatat gccaatgtct ttataaaatg gaccgttgca
    10021 gtgaattgca aagctatgga aaagcacttc tagacaaggg aggaaacaac aaaaatataa
    10081 atgatattaa acatcttctt ggtgcatgca gctttaaatc acagaattac gctgatgcat
    10141 tggaatactt gtctttatac aaaacagcta caaagaaaat aagtcgtgaa gattggtata
    10201 tgttaggaat atcggcatat aaaacagatg attttaaaaa tgccattgcc tatctatcaa
    10261 aagcaactag caaggaagac atattaacac aaaacagcta cttccatata gcaatggctt
    10321 acattaaact tggcgacaaa aagaacgcac gtatggcatt tgaagccgct gcaagataca
    10381 aatatgattc cggtatccaa gaagatgcac tctataacta cgctttagtt acttacgaga
    10441 tgtctttctc tccatttaac gaatccgtag ttgctttcga aagatttttg aaatatttcc
    10501 ctaactctca atacgttcca aaagtatacg aatatctact aaatgtgtac ctcactacca
    10561 aaaactatga tgcagcttat caatcaattc agaatattaa aagtgataat cctgctatca
    10621 aggaagctga gcaaagagta cttttcggaa tgggaacgac agcagttgcc aatcgtaaat
    10681 acggagctgc agccaaaaat tttgagacta taatcaacaa tagatcatac aattatgaga
    10741 ttacagcacg aagctatttt tggtatggag agtgcatgta taagatgaag aaatatgatg
    10801 aggcaaagaa agcttacgaa ggatacttaa ataaaatagg cagtaagaat ggtgatgagt
    10861 actctttagc tcattacaat ttaggctatg tatacattaa acaaaatgat tacaaagaag
    10921 caaatctgtg gttccgtaag ttcatcaatt tagagagcga agataaagtt atgttggtcg
    10981 atgcatgcaa cagaattggt gattg
//
LOCUS       DAOPID010000026        10998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_7246_length_10998_cov_4.436443, whole genome shotgun sequence.
ACCESSION   DAOPID010000026 DAOPID010000000
VERSION     DAOPID010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 10998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10998
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7246_length_10998_cov_4.436443"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PK641_01850"
     CDS             <1..440
                     /locus_tag="PK641_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="decatenates newly replicated chromosomal DNA and
                     relaxes positive and negative DNA supercoiling; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HPG55132.1"
                     /translation="EKFDSEKIWTAVLYDAEQKNPYIKRFKLEASTKEFSFIGEHPDS
                     RLIHITDTYYPLLEIKLQGAADNAEPLVLDADEYIGVKSYKAKGKRVATAMINEIREL
                     EPLRVPEEVDDSLPDNNEEDNEQNEDTDLSQQTPDNDGQFSLF"
     gene            458..874
                     /gene="tsaE"
                     /locus_tag="PK641_01855"
     CDS             458..874
                     /gene="tsaE"
                     /locus_tag="PK641_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPG55133.1"
                     /translation="MNKLLIKDLDSLHSVAKDFLKLIGDSKIIAFYGAMGAGKTTFIK
                     ALCDVLGVQDTVNSPTFAIVNEYTDDEDNPIYHFDCYRIKKIEEAYDLGYDQYFFSGN
                     YCFIEWPEMLEELLPEDTKKVIITERKDGGRDVLFD"
     gene            858..1394
                     /locus_tag="PK641_01860"
     CDS             858..1394
                     /locus_tag="PK641_01860"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HPG55134.1"
                     /translation="MCFSIDKKILRKEMKKRVACLGCATKQAKSKVIASLLEQNQHFK
                     NASSILFFWPLPDEIDLSRWIEEWAKTKQVYLPVVQEDNLIICKYTEQKIKGRYNIME
                     PVNSEIVDLSEIDLVVVPGLGFDDVTFARLGRGKGFYDKLLSNSTVYKIGICYSEQLC
                     KSLPIELHDVKMNEVIFA"
     gene            1476..2369
                     /locus_tag="PK641_01865"
     CDS             1476..2369
                     /locus_tag="PK641_01865"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HPG55135.1"
                     /translation="MSWNNNWRPKGKSVNKYLITKNNLIRLIAFTAVFSLAFINLYKP
                     FNSEKWYPVSSVGYFAFSFILVLTGIIVIAISRYVMYRFVRKHSLYNTEYALWIVLEI
                     VALSGFYTLYTIAVRDDLNFWSWEDIITVFKDASINTFLVIFLPYTVSWLFFSYTDKK
                     GRLKQLENSVLHPEPTIIQFHDEKGELRFSVSSENIIYLEAADNYVEINYLKQGKITQ
                     HLLRNSLKKLSSELDKTAIRRCHRSYMVNFEHVIALRRNNDEIDLEIDVPDIKKIPVS
                     KTHGDETTEAFMAFSGRQIKQ"
     gene            complement(2405..4051)
                     /locus_tag="PK641_01870"
     CDS             complement(2405..4051)
                     /locus_tag="PK641_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPG55136.1"
                     /translation="MIKHFLSYFEETIKKYWDAPALSDFDGDLTISYGQTAEAIAKLH
                     ILFKEAGIEKGDKIAICGRNSAHWAVSFLAITTYEAVAVSILADFSADSVHALVNHSD
                     SKFFMVGDVVWQGLDKDKMPNLLGIICMKDFELLYAANKKFEAKYKDRDNLFKSKYPQ
                     GFGVTDVSYPIDNLEDVALINYTSGTTSDPKGVVLSYKSISSNVQFGQDRIPNKPGWS
                     MVSMLPLAHMFGLTFEFLYQLAGGCHVYFLSKTPSPQVLMKAFAEVHPYMILTVPLVI
                     EKIFKKTIFPAIKKPIVRELWYIPGINTPIRRKVRDKLMQAFGGNLRHLIIGGAALNH
                     EVENCLKQIKFCYTVGYGMTECGPILGYEDWQNFKKRSCGKIVDRMTVTIDSNNPEKS
                     VGEIMVKGDNVMMGYYKNPEATAAIFTPDGWMRTGDLGIIDKDGNIFIRGRNKSVIIG
                     SSGQNIYPEEIEDKLNNIPGILESVVVDRGGYLTALVFPDNEALKEEQETKSMEVIMN
                     GILVKLNKLVPPFCKIKQIELVDKEFEKTPKRSIKRFMYK"
     gene            4416..6047
                     /locus_tag="PK641_01875"
     CDS             4416..6047
                     /locus_tag="PK641_01875"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HPG55137.1"
                     /translation="MAVTFKYQAPFPLGEDKTNYYLLTSDYVSVGEFEGKKILKIEKE
                     GLTAMARAAFRDVSFLLRRSHNEQVAKILSDPEASENDKYVALTFLRNAEVSAKGQLP
                     LCQDTGTAIIHGEKGQQVWTGYCDEEALSLGVFKTYTEENLRYSQNAPLSMYEEVNTK
                     CNLPAQIDLEATEGMEYSFLCVTKGGGSANKTYLYQETKALLNPSTLVPFLVEKIKTL
                     GTAACPPYHIAFVIGGTSAEKNLQTVKLASTHYYDNLPTTGNEGGRAFRDIELEEQVL
                     SEAHKIGLGAQFGGKYLAHDVRIIRLPRHGASCPVGMGVSCSADRNIKCKINEDGIWI
                     EKMDDNPGELIPEELRTAGEGNAVKINLNQPIANVLAELTKYPVSTRLSLSGTIIVGR
                     DIAHAKLKERIDKGEGLPQYMKEHPIYYAGPAKTPSGMACGSMGPTTAGRMDSYVDLF
                     QENGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAILAQNNIKNIKCLEYPELG
                     MEAIWQIEVEDFPAFILVDDKGNDFFKQIKPCCCR"
     gene            complement(6078..>6489)
                     /locus_tag="PK641_01880"
     CDS             complement(6078..>6489)
                     /locus_tag="PK641_01880"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPG55138.1"
                     /translation="TLTKPYDGIPELLTYLQSEGIKIAVASNKFIAGTQALISHYFKQ
                     FNFAVVLGQREGIPAKPNPTIVNEILSVTGVDKKDVLYLGDTSIDMQTAKNAGVFAIG
                     VSWGFRTKQELLDNGANLIIDHPSELITQIKMLR"
     assembly_gap    6490..6499
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6500..6748)
                     /locus_tag="PK641_01885"
     CDS             complement(<6500..6748)
                     /locus_tag="PK641_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HPG55139.1"
                     /translation="MVQLVIFDLDGTLLNSVADLGQATNIALKKHDLPTHELDCYKYF
                     VGNGVSKLIERAVPEEMRIESLLAEVKADFMNYYMEHKT"
     gene            complement(6824..7645)
                     /gene="tsf"
                     /locus_tag="PK641_01890"
     CDS             complement(6824..7645)
                     /gene="tsf"
                     /locus_tag="PK641_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPG55140.1"
                     /translation="MAVTMADISKLRTMTGAGMMDCKNALTEAENDFEKAIEIIRKKG
                     KAIAAKRSDREASEGCVLATDKGGFAAIVALQCETDFVAKNADFIALTQSILDAAMAN
                     CPASLDELKAIKIDGRSIEDLVIDRSGITGEKMELGSYEFVKGNTTISYIHPGNKLAT
                     IVSFNENIDHQISREVAMQVAAMSPVALNREQVPAHIVETELEIGRDKARQEGKPEAM
                     LDKIAEGRLNKFYKESTLLEQEFIKDAKISVQQYLKQQSATLTATGFKRVTLNQE"
     gene            complement(7648..8505)
                     /gene="rpsB"
                     /locus_tag="PK641_01895"
     CDS             complement(7648..8505)
                     /gene="rpsB"
                     /locus_tag="PK641_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPG55141.1"
                     /translation="MPRTNFQDLLEAGVHFGHMKRKWNPAMAPYIFMEHNGIHIIDLN
                     KTIVKIDEAADALSLIAKQGKKILFVATKKQAKDIIAEKAGSVNMPYITERWAGGMLT
                     NFTTIRKAVKKMTAIDKMSTDGTFENLSKREKLQISRQREKLEKNLGSIADLTRLPAA
                     LFVVDVMKEHIAVQEANSLGIPVFGIVDTNSNPNDIDYVIPANDDASKSIDIILTAVT
                     AAIQEGLQERKLEKETEAAENAEAGTSEKKEKGGTGKQGRSRIKRGNEDALNAKLAEK
                     YLTDAEKEG"
     gene            complement(8654..>8812)
                     /gene="rpsI"
                     /locus_tag="PK641_01900"
     CDS             complement(8654..>8812)
                     /gene="rpsI"
                     /locus_tag="PK641_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPG55142.1"
                     /translation="LRLAIARALVKINPEDKPALKSEGFMTRDPREVERKKPGRPKAR
                     KRFQFSKR"
     assembly_gap    8813..8822
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8823..9039)
                     /gene="rpsI"
                     /locus_tag="PK641_01905"
                     /pseudo
     CDS             complement(8823..9039)
                     /gene="rpsI"
                     /locus_tag="PK641_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545814.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
     gene            complement(9046..9501)
                     /gene="rplM"
                     /locus_tag="PK641_01910"
     CDS             complement(9046..9501)
                     /gene="rplM"
                     /locus_tag="PK641_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPG55143.1"
                     /translation="MDTLSYKTISANKATVTKEWVVVDATDQIMGRFCSKVAKLARGK
                     YKPNFTPHVDCGDNVIIINADKVKMTGKKWTDQISYLYSGYPGGQREMNPKRLQEKGS
                     KYFFAKVLKGMLPKNKLGDVILSNVYVYDGAEHPHQAQNPKTIDINALK"
     gene            complement(9682..>10998)
                     /locus_tag="PK641_01915"
     CDS             complement(9682..>10998)
                     /locus_tag="PK641_01915"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HPG55144.1"
                     /translation="VKSELENLSLKYRSPHEYARIEKQLSDYINAHTKSVEEWMEPIR
                     KALYDNGINATVSCDPRSVYSLWYKMHQTKLTFKELEHIRIIQICYDAEKNNTISEKD
                     QALRVYSILTDLYTEKPFSIINYIDMPKENGYKSLHCKLMGNEGRWMEVHIQSKEMRR
                     VSRYGCLVERETGVEAWIAKFRDVLRDIADQSKDTQSLEDVVSSFYHDDIVVFAANGN
                     KITLPKGATAIDFAYEIHSDVGEKAKYALINDKLCSIKTVLKRGDRVKIGTDNDANPK
                     ADWLRYAQTYRAKKHIRQYLNKQEQIDYTPYILCDDCHPLPGDEVVGFHRPDGSIFVH
                     KCNCRQSILLSSQEGDVIEHVTLKPSTHRTYPATLKINAVNRNNFLLDLMNEISNKLG
                     LSIESIESYTNDEIIYCQIKLYVHSVEELNAAINDIQQLKNVYEVK"
BASE COUNT         3326 a         1866 c         1961 g         3825 t
ORIGIN      
        1 tagagaaatt tgattcggag aagatttgga cagcagtttt gtatgatgca gaacaaaaga
       61 atccatatat aaagcgtttt aaactggaag cttcaacaaa agagttctca tttataggag
      121 aacatcccga ttctcgctta atacatatta ccgatacgta ttatccgctt ttggaaatta
      181 aattacaagg tgctgctgat aatgcagaac cattagtttt agatgcagat gaatacatag
      241 gcgtaaaaag ctataaggcg aaaggcaaac gtgttgcaac cgccatgata aatgaaatac
      301 gcgaacttga accattacgt gtacctgaag aggtagatga ttctttgccg gataataatg
      361 aagaagataa tgagcaaaat gaagatacag atttatctca gcaaacgcct gataatgatg
      421 gtcaatttag cttgttttaa ttatttatta gaaacaaatg aataaacttt tgataaaaga
      481 tttagatagt ctgcactctg ttgcaaaaga ttttcttaag ctgataggtg attctaagat
      541 tattgcgttc tatggtgcta tgggtgcagg taaaactact tttataaaag cattgtgtga
      601 tgtattgggt gttcaagaca cagttaatag tcccacattt gcaatagtga acgaatatac
      661 tgatgatgag gataatccga tttatcactt cgattgttat agaataaaaa aaatcgaaga
      721 agcttatgat ttaggttacg atcagtattt tttcagcggt aattattgtt ttatagaatg
      781 gcctgaaatg ctcgaagaat tacttcctga agatacaaag aaagttatta ttactgagcg
      841 taaagacgga ggtagagatg tgcttttcga ttgacaaaaa aatattgcgg aaagaaatga
      901 aaaagcgtgt tgcatgtttg ggctgtgcaa caaaacaggc taaatcaaag gtaattgcat
      961 cacttttaga gcaaaatcaa cattttaaaa atgcatcctc aatactattt ttttggccat
     1021 tacccgatga aattgattta agccggtgga tagaagaatg ggctaagact aaacaagttt
     1081 atttgcctgt agtacaggaa gataatttaa ttatctgtaa atacacagag caaaaaatca
     1141 aaggaagata taatataatg gagcctgtaa attcagaaat tgtagattta tctgagatag
     1201 atttagtagt tgtaccgggc cttggatttg atgatgttac ttttgcacgt ttaggacgcg
     1261 gcaagggttt ttatgataaa ttattatcta attctactgt gtataaaata gggatttgtt
     1321 attctgaaca attatgcaaa agtttaccta tagagttgca tgatgtaaag atgaatgaag
     1381 taatttttgc ttaaacaaaa aaattcttac ctttgcccac gctcgctatg aattgtttgc
     1441 gctttacaaa ttattaatgg aaaaaatcat tgattatgag ttggaataat aattggaggc
     1501 ctaagggaaa gtcggtaaat aaatacttga ttaccaaaaa caacctaatc aggcttatag
     1561 cctttactgc tgttttttcg ttggcattta taaatttata taaaccattt aattctgaaa
     1621 aatggtatcc tgtatcttca gtagggtatt tcgcattctc atttatatta gtactgacag
     1681 gtataatagt tatcgcaata agtagatatg taatgtatcg ttttgtgcgt aaacattcat
     1741 tgtataatac tgaatatgcc ttatggatag tacttgagat agttgcttta tcaggatttt
     1801 atacattata tacaattgct gtcagagatg atttgaactt ttggtcgtgg gaagatatta
     1861 ttaccgtttt taaagatgcc agtattaata cctttttagt tatatttttg ccttacactg
     1921 tttcgtggct attcttttca tacacagata agaaaggtcg cttaaaacaa ttagaaaata
     1981 gtgtgttgca tccggaacct actattattc agtttcatga tgaaaaaggc gaattgagat
     2041 tctctgtatc atccgagaat ataatatact tagaagctgc cgataattat gtagaaatta
     2101 attatttaaa gcaaggtaag attacacaac atttgttgag gaattcactt aagaaattaa
     2161 gttcagagtt agataaaact gctataaggc gttgtcatcg ttcgtatatg gtcaatttcg
     2221 aacatgtcat tgcacttaga cgtaataatg atgaaattga tttagaaata gacgtgcctg
     2281 atattaaaaa gattcccgtt tcaaaaactc atggtgatga aacgaccgaa gcatttatgg
     2341 ctttttcagg tcgtcaaatt aaacaataat ggcagagatc ttgtctctgc catcaccaat
     2401 atacttattt gtacataaac ctcttaatac tgcgtttagg ggtcttttca aattctttat
     2461 caactaattc tatttgtttg attttacaga atggaggtac taatttgttt aattttacca
     2521 agatgccatt cataataact tccatcgatt ttgtttcttg ttcttcttta agagcctcgt
     2581 tgtcaggaaa tactaaagct gtaagatatc cacctctgtc aacaacaaca gattctaaaa
     2641 ttcccggaat gttatttagt ttatcttcaa tttcttcagg atatatattt tgacccgatg
     2701 aacctataat aacactctta ttgcgtccac gaataaatat atttccatct ttatcgataa
     2761 ttcctaaatc accggtacgc atccatccgt caggagtaaa gatagcagca gttgcttcag
     2821 ggttcttata ataacccatc atcacattat cgcccttaac cataatttcg ccaacactct
     2881 tttccggatt attagaatct attgtaactg tcattctatc gacaattttt ccgcatgagc
     2941 gttttttgaa attttgccaa tcttcataac ctaatatcgg gccacattct gtcattccgt
     3001 agcctactgt atagcagaat ttaatttgtt ttaggcaatt ttctacttca tgatttaaag
     3061 ctgcacctcc aataataaga tggcgtaaat ttcctccaaa ggcctgcatt aatttatccc
     3121 taaccttgcg tcttatagga gtattaattc ccggtatata ccataattct cttacaatcg
     3181 gttttttgat tgcagggaat atagttttct tgaaaatctt ttcgattact aaaggcactg
     3241 tcaaaatcat gtacggatga acttctgcaa aagctttcat caaaacttga ggagatggtg
     3301 ttttgctcaa gaaataaacg tggcaacctc ccgctagttg atacaaaaac tcaaatgtta
     3361 atccaaacat atgtgcaagt ggaagcatag aaaccatgct ccaaccgggt ttgttaggaa
     3421 ttctatcttg tccgaattga acattagaac tgatactctt ataacttaga actacgcctt
     3481 tcggatcgct ggttgtacct gaagtataat taattagtgc aacatcctct aggttgtcaa
     3541 taggatatga aacatctgtt actccaaatc cttgtgggta tttagatttg aatagattat
     3601 ctctatcctt gtattttgct tcaaattttt tgttagcagc gtacaaaagt tcgaaatctt
     3661 tcatgcaaat aatacctaac aagttaggca ttttatcttt gtctaaacct tgccaaacta
     3721 catctccaac catgaagaat tttgaatccg aatgattaac taaagcatga acgctatctg
     3781 cagaaaaatc tgcaagtatt gatactgcca cagcttcgta tgtagtaatt gctaagaagc
     3841 ttacagccca gtgtgctgag tttctaccgc aaatggcaat cttgtcacct ttttcaatgc
     3901 ctgcttcttt aaacaatatg tgcagtttag caatggcttc tgctgtttgt ccataactaa
     3961 tagttaagtc tccatcaaag tcacttaatg caggtgcatc ccagtatttt ttgattgttt
     4021 cttcaaaata ggagagaaaa tgtttaatca tatatttttg tttagtttgt tgaatgataa
     4081 aagcataata acaaagcagg caaattttat tctacctgat ttattatatt ttgtcttcga
     4141 atatgatcat ttttcacaaa gatatataat aacatgatac cataatacag aaagatatat
     4201 aaaggtttat ttatgcttat ttgtaagtaa aaacatgtat tatggtacta aaaataacat
     4261 agaggggtaa aattattcac tataaagccc atttgccttg attttttaaa aataacagta
     4321 gctgatggca ttaaaaatta tctgtcttaa ttgctgagat acggctattt tatagtaatt
     4381 ttacaagctg atatttaatc taaaaaaagc atattatggc agttacattt aagtaccaag
     4441 ctccattccc tttgggagaa gataagacaa attattactt attgactagt gattatgtgt
     4501 ctgtcggcga atttgagggt aaaaagattt taaagattga gaaagaaggt cttacagcaa
     4561 tggcgagagc ggcttttcgt gatgtgtcat ttttgttgag acgttctcat aacgagcagg
     4621 tagctaaaat tttaagtgat cctgaggcaa gtgaaaacga taaatacgta gctttgactt
     4681 ttttgcgtaa tgcagaagtt tcagctaaag gacaacttcc gttgtgtcaa gataccggta
     4741 cagctattat tcatggcgaa aaaggacaac aggtttggac cggttattgt gacgaagaag
     4801 ctttgtcatt aggtgttttt aaaacatata cagaagagaa ccttagatat tctcaaaatg
     4861 ctccgctatc aatgtatgaa gaagtaaata ctaaatgtaa tcttcctgcg cagattgatt
     4921 tggaagctac tgagggtatg gaatatagct ttttgtgcgt aactaagggt ggtggatctg
     4981 ccaacaaaac atatttgtat caagaaacca aagcattgtt gaatccttca actttagttc
     5041 cttttttggt agagaaaatt aaaactttag gtacagctgc ttgtccacca tatcacattg
     5101 catttgtaat tggaggaact tctgctgaaa aaaatcttca aacagtgaaa ttagcatcaa
     5161 ctcattatta tgataatctt ccaactacag gtaatgaagg aggacgagct tttagagata
     5221 ttgaactgga ggagcaagtt ttatctgaag cgcataaaat aggtttaggt gcacaatttg
     5281 gtggcaaata ccttgcacat gatgtaagaa taattcgttt accgcgtcat ggtgcatctt
     5341 gtccggttgg tatgggcgtt tcttgttcag ccgatcgtaa tattaaatgc aagattaatg
     5401 aagatggtat ttggattgag aaaatggatg ataatcccgg agagttaatt ccggaagaac
     5461 taagaactgc aggggaaggt aatgcagtta agattaatct taaccaacca attgcaaatg
     5521 tattagcaga acttactaaa tatcctgtat caacacgttt gtcattaagt ggtacaatta
     5581 ttgttggacg tgatattgca catgcaaaat taaaagagcg tattgataaa ggtgaaggct
     5641 tgcctcaata tatgaaagaa catcccattt attacgcagg accggctaaa accccttcag
     5701 gaatggcatg cggctctatg ggacctacaa cagccggaag aatggattca tatgttgatt
     5761 tattccaaga aaatggcggt agcatgatta tgttggcaaa agggaataga agtcaacagg
     5821 ttaccgatgc ttgtaaaaaa catggtggtt tctatttagg ctcaatagga ggtccggctg
     5881 caatcttagc tcagaataat ataaagaata ttaagtgtct tgaatatcct gaattaggga
     5941 tggaagctat atggcagata gaagttgagg acttccctgc atttattttg gttgatgaca
     6001 agggtaatga tttcttcaaa caaattaaac catgttgttg tagataatct atgaacacaa
     6061 ataaaaatgc tcagatacta tctgagcatt tttatttgtg taatcaattc tgacggatgg
     6121 tctataatta aatttgcgcc attgtctaag agttcttgtt ttgttctaaa tccccaactg
     6181 actccaatag caaatacacc tgcattcttg gctgtttgca tatctattga agtatcgcct
     6241 aagtataaga catctttttt atcgactcct gtaacggata atatctcgtt tactattgtt
     6301 gggttaggtt tggcaggaat accttctctc tgtcctaata caacagcaaa attaaattgt
     6361 ttaaaataat gggagattag tgcttgtgtg ccggcaataa atttgtttga tgcaactgct
     6421 attttgatgc cttcagactg taaataagta agcaattcag gaattccatc gtaaggcttg
     6481 gtaagcgttn nnnnnnnnnt gttttatgtt ccatataata gttcataaag tctgctttta
     6541 cttcagccaa aagactctca attctcattt cttcaggaac tgctctttca attaatttgc
     6601 ttactccgtt gccaacaaaa tatttgtaac aatctagttc gtgtgttggt aggtcatgct
     6661 tttttaatgc aatattagtg gcttggccaa ggtctgcaac agaattaagt aatgtcccgt
     6721 ctaaatcaaa tattactagt tgtaccatta ttatttgctt aaaataaaaa actccgtcta
     6781 attatgacgg agctttttat gtaatgtgta agaataataa taattactct tgatttaatg
     6841 ttacacgttt aaaaccagta gcagttaatg ttgctgattg ttgtttcaag tattgttgaa
     6901 cactgatttt tgcgtcttta ataaattctt gctccaacaa ggtagattct ttgtagaatt
     6961 tatttaaacg tccttcagca attttatcaa gcatagcttc aggtttacct tcttgacgtg
     7021 ctttatcacg accaatttcc aattctgttt caactatgtg tgcaggtacc tgttcgcggt
     7081 taagtgctac agggctcatt gctgcaactt gcatagcaac ttcacgtgaa atttggtgat
     7141 ctatattctc attgaaagaa acaattgtag ctaatttgtt tcccggatgg atgtatgaaa
     7201 tagtagtatt cccttttaca aattcatagc ttcctaattc cattttttca ccggttatac
     7261 cactgcggtc tataactaag tcttcaatgc tgcgtccgtc gattttgatt gcttttaatt
     7321 catctaggct tgcagggcag tttgccattg cagcgtctaa tatgctttga gtaagtgcaa
     7381 taaaatctgc gttttttgcg acgaagtcag tttcgcattg caaagcaacg atagcagcaa
     7441 agccaccttt gtcagtagct aaaacacaac cttctgaagc ttcgcgatca ctacgtttag
     7501 cagcaatagc tttacctttt ttgcgaataa tttctatagc tttttcaaaa tcattttcgg
     7561 cttctgtcaa agcgtttttg caatccatca ttccagctcc ggtcattgtc cgaagtttag
     7621 aaatatctgc cattgtaact gccataatta accctccttt tctgcgtctg ttaaatactt
     7681 ttctgccaat tttgcattca aggcatcttc attacctctt ttaatgcgag agcgtccttg
     7741 tttaccggtt ccgccttttt ctttcttttc gcttgtgccg gcttctgcat tttcagcagc
     7801 ttctgtttct ttttctaatt tgcgttcttg caatccttct tgaatagcag cagtaactgc
     7861 agtcaatata atatcaattg attttgatgc atcgtcgttt gcagggataa cataatcaat
     7921 atcattagga tttgaattag tatcaacgat tccaaagaca ggaatcccca aactattagc
     7981 ttcttgtaca gctatgtgct ctttcatgac atcaactacg aataaagcag caggtagacg
     8041 agttaaatct gcaatactac caaggttctt ttctaatttt tcgcgttgac gagatatttg
     8101 taatttttct cttttagaaa ggttttcaaa agtaccatct gttgacattt tgtcgatagc
     8161 agtcattttt ttaactgctt tacggatggt tgtgaagtta gtcaacatac cacctgccca
     8221 acgttctgtg atatatggca tattaactga acctgccttt tctgcaatga tgtcttttgc
     8281 ttgtttcttt gtagctacaa acaaaatctt tttgccttgt tttgctatta aagataaagc
     8341 gtcagcagct tcgtctattt ttacaattgt tttgtttaag tcaataatgt gaataccatt
     8401 atgttccata aatatgtatg gagccattgc aggattccat ttacgtttca tgtgtccgaa
     8461 atgaacaccg gcttctagta agtcttgaaa atttgttcta ggcatgtttt ttttattaag
     8521 ttgtttacat tcttgtgatt acatatcaac tatctagtag tctcaagaat ggagagtcta
     8581 gatagtttag atactaaaca actctttcca aatctgtctc aaattgaaat ggatttggaa
     8641 agagatatag atattaacgt ttactgaatt ggaaacgctt acgtgctttt ggacgtcccg
     8701 gtttcttacg ttcaacctcg cgtgggtcac gtgtcatgaa gccttcagat tttaaagcag
     8761 gtttgtcttc cggattaatt tttactaatg cgcgtgctat tgcaagacga agnnnnnnnn
     8821 nngacctttg caaccgccac cgtttaaatt aactttgata tcataatttt ctgttacgcc
     8881 caatttggaa aggggttgtc ttactacata ttgaagtatg cctgaaggaa aatatgctgc
     8941 aaattcacga ttgttgatgg tgattgcacc ttttcctgtg gtaacataaa cgcgcgctac
     9001 tgcggctttt cttcttccta ttgcatttgt tacttccata ttcctttatt taagagcgtt
     9061 gatgtcgatt gtttttggat tctgtgcttg atgagggtgt tctgcaccat catatacata
     9121 tacgtttgaa agtattacat ccccaagttt atttttgggt aacattcctt tcaatacttt
     9181 tgcgaagaaa tattttgatc ctttttcttg taagcgttta ggattcattt cgcgttgtcc
     9241 gcctggatat cctgaataca aatatgaaat ttgatctgtc cattttttgc cggtcatttt
     9301 gactttgtca gcattaatta ttataacgtt gtcaccacaa tcaacatgag gagtaaaatt
     9361 tggtttatac ttgcctcttg ccaatttagc aaccttggag cagaaccgtc ccataatttg
     9421 gtcggtagca tccacaacca cccattcttt tgtaacggta gctttgtttg cggaaatagt
     9481 tttataactt agagtatcca cttttattaa atttattcgt ttacaacttg tcgttttact
     9541 tttggtacaa aggtcttaaa ggctaagaaa gaccttatac attgtaagcc aacgacttag
     9601 cttaaaaaga acaataatcg gactgcaaaa atacgctatt ttcctgaaac ataaaaactt
     9661 ttgaacagtt ttgtttaaaa attattttac ctcgtataca ttcttgagtt gttgaatgtc
     9721 attaatagca gcatttagtt cttcaaccga gtgaacatac aatttaattt ggcagtatat
     9781 tatttcatca tttgtgtaac tttcgataga ttcgatactt aaacctaatt tgtttgagat
     9841 ttcgttcatt aagtctaata agaagttatt tctgttaact gcatttattt tcagggtagc
     9901 aggatatgtt ctgtgtgtcg atggcttgag cgttacgtgt tctattacat cgccttcttg
     9961 tgatgataat aatatagatt ggcggcaatt gcatttatgt acgaaaatac tgccgtcggg
    10021 tctgtgaaat cctacaacct cgtcaccggg aagaggatga caatcatcac ataatatata
    10081 tggtgtataa tcaatttgtt cttgtttgtt caaatattgg cgaatatgtt tctttgctct
    10141 atatgtttgt gcatatctta gccaatctgc cttaggatta gcatcattgt cagtacctat
    10201 ctttactcta tcacctcttt ttagcactgt ttttattgaa cagagtttgt cgtttattaa
    10261 tgcatactta gctttttctc ctacgtctga atgtatttcg tatgcaaaat ctattgcagt
    10321 agctcctttc ggcaatgtta ttttatttcc gtttgcagca aaaacaacta tgtcatcatg
    10381 ataaaaacta ctcacaacat cttctaaaga ttgtgtgtct ttgctttggt ctgcaatatc
    10441 gcgaagtaca tctctgaatt ttgcaatcca cgcttcaact cctgtttctc gttcaactag
    10501 gcatccataa cgcgacactc tgcgcatctc cttagattgt atatgcactt ccatccatcg
    10561 cccttcgttt cccatcagtt tgcaatgtaa actcttgtag ccattctctt ttggcatatc
    10621 aatgtagttt attatgctaa aaggtttttc tgtgtataaa tctgtcaaaa tggagtaaac
    10681 acgtaatgct tggtcttttt cactaattgt gttatttttt tcagcgtcat aacatatttg
    10741 aataatacgt atatgctcaa gttctttaaa tgtaagttta gtttgatgca ttttgtacca
    10801 taatgagtac acgctacgtg gatcacaact tactgtagca ttaataccat tatcatacaa
    10861 ggcctttcta atcggctcca tccattcttc aacagatttg gtatgggcat ttatatagtc
    10921 agatagttgt ttctcgatgc gtgcatactc atgtggggaa cgatatttta agctaagatt
    10981 ttctaattct gatttaac
//
LOCUS       DAOPID010000027        10994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_7251_length_10994_cov_4.463296, whole genome shotgun sequence.
ACCESSION   DAOPID010000027 DAOPID010000000
VERSION     DAOPID010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 10994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10994
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7251_length_10994_cov_4.463296"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /locus_tag="PK641_01920"
     CDS             <1..1104
                     /locus_tag="PK641_01920"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPG55145.1"
                     /translation="KNAVVYIQAFQDLLLEYVSKQSSDANRFVEWWEQTGISKSLTTG
                     DAGNAVTIMTLHKSKGLSFNVVIMPFCTWKLDDYSSINTKIMWCKTSSPFDDMDYVPV
                     KYSKALSKTIFAEDYYKELMNNYMDSINLMYVAFTRPIKALICFGMNPKKDSFNNVGD
                     LLRKYINSPNLSEFVSSDETSAKFIQGELKPKESDVTKPLDEGSEDIYITHTDISRFG
                     LRKKSDSSEQNIMGIILHGIMEKIIKSEDLNSVVQEMQRLGLVSADEAKQITEKLDPY
                     INNPAVKTWFDGSYKILNERTIITADAENYRPDRIMIGKDEIIVVDYKFGEKEDSYFS
                     QVSTYMSLLKSMGYKNVSGYIYYQIDLEIVTVS"
     gene            complement(1081..1788)
                     /locus_tag="PK641_01925"
     CDS             complement(1081..1788)
                     /locus_tag="PK641_01925"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPG55146.1"
                     /translation="MPNDYFKFKRFTIMQSDCAMKVGTDGVLLGAYTQIDSDEQILDI
                     GTGTGLIALMLAQKSTSSTIDAIEIDEKAANQARNNIMASPWKNINIICVDFIQYAEN
                     CDKQYSLIISNPPYFKQSLKAPEHERSIARHNDILPFDKLCSSVAKILSDNGRFCLIL
                     PTIEAEIFIKEAEKYSLFVTKCTKIIPKPGAKVIRLILELTKNKQDLTEHQLCIETDT
                     RHEYTEEFKKLTDSYYL"
     gene            complement(1906..3237)
                     /locus_tag="PK641_01930"
     CDS             complement(1906..3237)
                     /locus_tag="PK641_01930"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPG55147.1"
                     /translation="MKTITKRFFSAVTMIMVGFAAFAAPASEWCDTPTGHLNDPNFGD
                     VNSHILLTISGDEAGKLNIVVKPGLDNVKGINYIYVTVSDATQGFYEFGTAQTGEPLA
                     ELAIEATLNPGTTTSSIMVQYGNPDWAGRWQVSVSDIDVTANCSGDCDLTENPTMTSA
                     SLESKTAYDINLNVAGVDQNSNAITNFVVSCASFTDKVFTATDGVIKIDGLTPGTEYN
                     FSIKAKDYCNNISTGAESVIASTETLESQCSGDKGHFGNPTVLRIHYVISYDAISGNV
                     TYTVTPYNSELTLMYAEIQQTEGGNQVMTIANDEKSATYTTQYTGESVKSLFLYRTSD
                     IPGNEMTAENLTKPEAIYYKVGDCPATSVQETSTAIAKISPNPAVQFVNIVSQEEISK
                     IEVRAQNGAVVISIKPNSNECNLDIAHLAKGRYFVTIYNGAVFNTETMVVK"
     gene            complement(3514..4938)
                     /locus_tag="PK641_01935"
     CDS             complement(3514..4938)
                     /locus_tag="PK641_01935"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 protein"
                     /protein_id="HPG55148.1"
                     /translation="MRIAHLIITTACISLSMICQAQTATVYTTAMANDTRYQKTIISP
                     IKSEQPTEDKYSVYVDPLHHFQKYIGFGGAITDASAEVFAKLPKDAQEELLSAYFDKE
                     NGLAYNIIRSNIQSCDFSPYSYSYWEENDISLSSFSIEHDKQYRIPLIKKAIKSIGNG
                     SIFYVAPWSPPLWMKDNRSIIRGGKLLPEYRQLWAECFVQFIKAYEAEGIKVWGLSTQ
                     NEPMATQTWESCIYTAQDECDFIKNNLGPTLKKNKLGDKKIIIWDHNRDLIYHRAATI
                     LNDPEAAKYVWGVGFHWYETWTKSPMYFENLRRVKESFPDKELIFTEGCVEKFDLNNI
                     NDWSIGERYGYSMINDFNCGTSAWTDWNILLDEKGGPNHVQNYCFAPIHGNTKTGKLM
                     YTNSYYYIGHFSKFIKPGAVRIACASSRAELYCTAAMNPDKSIVVICMNKEDKEIEFS
                     MQMRDKTYNCILPAHSISTTCIKK"
     gene            complement(4964..6091)
                     /locus_tag="PK641_01940"
     CDS             complement(4964..6091)
                     /locus_tag="PK641_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55149.1"
                     /translation="MKSIASSIILLLSLATLSSCHDQPTPGSRKIYINSEYGNLKEVI
                     VGLASGKNPSIDAPWYKEAIKILPQDERDIAEATAGYNWTDIKYDDQGKYDPTGTISE
                     AQRIDSENNELIAVFESLGVTVYRPDLITEEWVIENYGEAALINGYSQDFPRDNLAII
                     GNNVIELTLKTPLRRFDIIGFNHLLTTKCDESVVWVSMPQIPLLPVPSDETPALEGGD
                     VMVLGKTILVGNTANKAVGSNEAGYKWLKNYLGDDYKVIRVPLVETILHLDCALSIPK
                     EGLAIICKDAFSDGIPEALNGWDFIEVSLEEASRLAVNGLPVNDKNYIMSYNKHTEVT
                     SDRIKSALESHGITVHKVFFGAHNGQGGSIRCATQAILRDK"
     gene            complement(6228..7196)
                     /locus_tag="PK641_01945"
     CDS             complement(6228..7196)
                     /locus_tag="PK641_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016420840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HPG55150.1"
                     /translation="MIQNFADWLVYVVFGLKEGSSLGTAVNFFFYDTIKILILLFLIS
                     VLMGIINAYFPIDRVRNYLTKHKMFGLQYFLAATLGAITPFCSCSSVPLFIGFVKGGI
                     PMGVTMTFLISSPLLSEIAIAMFLGTFGLKITIIYVVSGLILSMIGGLILERFHLERL
                     LSPWVLELQKKSVEETEEWESEKISFWKRLPNIVLGSWRIVRSVFIYIIIGIGLGAAM
                     HGFVPDGFFESILSSDKWYSVPLAVIVAVPIYANAAGVVPIVEVLVQKGISMGTAIAF
                     MMAVIGLSFPEAMLLKTVMTWKLIGIFFGVTALLIIISGWLFNWLM"
     gene            complement(7198..7887)
                     /locus_tag="PK641_01950"
     CDS             complement(7198..7887)
                     /locus_tag="PK641_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HPG55151.1"
                     /translation="MIDSSNIPILIAFLLGLLTAVSPCPLATNITAIGYISKDIASKK
                     RVLLNGLLYTLGKIIAYTLLGAILIYILREGASTFHIQKTVSKYGELFLPFILLLLGL
                     FILFSDKIKLPSFGFKGNSESLKNRGGWGALFLGMLFALAFCPSSGVFYFGMLIPLSA
                     TSSGGYFLPAVFAFATALPVIIVAWIIAYSANSIGKFYGKMLVFQKWFNIVVGILCIT
                     IGIYYFVTIYL"
     gene            complement(7905..8312)
                     /locus_tag="PK641_01955"
     CDS             complement(7905..8312)
                     /locus_tag="PK641_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008023060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HPG55152.1"
                     /translation="MKRLIYLIIALTMFVSCGNAETKKQTKKIKSNTVEVLYFHGKQR
                     CITCVSIESLTKEVLEKDFKKELNNNKIVYKVIDITENEELADKYEVAWSSLILDKKG
                     KTVNLTDMGFSYAKNQPDVFKAKLKDELYKILK"
     gene            complement(8309..8557)
                     /locus_tag="PK641_01960"
     CDS             complement(8309..8557)
                     /locus_tag="PK641_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018462484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPG55153.1"
                     /translation="MKIKILGTGCAGCKALFETVQKAVSEMNIQAEIIKEEDLMKIMQ
                     YNVMSLPAIVVDEKVVAKGRLSLSEIKSLLKELQEGKK"
     gene            complement(8628..8945)
                     /locus_tag="PK641_01965"
     CDS             complement(8628..8945)
                     /locus_tag="PK641_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPG55154.1"
                     /translation="MTKELNNEQIARFAKALGHPTRIAILRFLDNRTTCYFGDIFEEL
                     PIAKATVSQHLKELKDAGLIQGEIEAPKVKYCINRDNWKLAKSLFVELFDDCVCKKGG
                     CCK"
     gene            complement(9071..9631)
                     /locus_tag="PK641_01970"
     CDS             complement(9071..9631)
                     /locus_tag="PK641_01970"
                     /inference="COORDINATES: protein motif:HMM:NF017927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HPG55155.1"
                     /translation="MACDSLKGNWSMPVLSTFLILLMSAMTAIPFGGIIVSIFFFVPV
                     CYSLTQAYLIFIRGDKDNLIARMFDCFKNYGRALSVSLLQAIYIILWTLLLFVPGIIK
                     SYSYSQAFFIALDKPELGAEECINESMRMMNGYKMKLFLLDLSFIGWWLLSILSLGIG
                     FLWLLPYIYTSHSHFYEELKAKTANQ"
     gene            complement(9703..10185)
                     /locus_tag="PK641_01975"
     CDS             complement(9703..10185)
                     /locus_tag="PK641_01975"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HPG55156.1"
                     /translation="MTTKILFVCLGNICRSPAAEGIMLHLIKEKGCSELFEIDSAGII
                     DYHQGELPDSRMRTHAMKRGYNLTHRARRITIDDFYNFDLILGMDDGNIDALQELAPT
                     LETKNKISRMTDYCVNIIADHVPDPYYGGAGGFEYVLDILEDACNGILSKKCHKILIF
                     "
     gene            10345..10929
                     /locus_tag="PK641_01980"
     CDS             10345..10929
                     /locus_tag="PK641_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HPG55157.1"
                     /translation="MDILKISEQNQKTAWKILEDTRIIKAWESIGATVNIIGSLKTGL
                     MMKACDIDMHIYTDKLDIAESFSVMQSLAEKLQFKEIHYINGIDTEEECIEWHVLYEN
                     NDMNVWKMDMIQIRKGSKYDGTVENVTNAIINKLTPDIRQTILQIKYDIPDNIKIPGI
                     EIYHAVFSGKVKTYEELEQWRKTNPLTNSLDWLP"
BASE COUNT         3442 a         1903 c         1861 g         3788 t
ORIGIN      
        1 aaaaatgcgg tggtatatat acaagctttt caagatttgc tacttgaata tgtttcaaaa
       61 caaagttcag atgcaaatag atttgtagag tggtgggaac aaacgggaat atctaagagt
      121 ttgactacag gtgatgccgg taacgctgtt actataatga cattgcataa atccaaaggt
      181 ttgtctttta atgttgttat tatgcctttc tgtacatgga agttggatga ttattccagt
      241 ataaatacaa aaataatgtg gtgcaaaaca agctcgccat ttgatgatat ggattatgtg
      301 ccggtaaaat attcgaaagc actgtctaaa acaatctttg ctgaagacta ttacaaagag
      361 ttgatgaaca attacatgga tagtattaat ttgatgtacg ttgcgtttac tcgtcctatt
      421 aaggcattga tttgttttgg catgaatcct aaaaaagatt catttaataa tgttggagat
      481 ttattgagaa aatatattaa ttcacctaat ctgagtgaat ttgtatcatc cgatgaaact
      541 tcggctaaat ttattcaagg agagttaaaa ccgaaagaaa gtgatgtaac aaagccgctt
      601 gatgaaggtt ctgaagatat atatatcact cacacagata tatctcgttt cggtttacga
      661 aaaaaatcag atagttctga acaaaatatc atgggaataa tattgcatgg tataatggaa
      721 aaaataatta aatctgagga tttgaattct gttgtacaag aaatgcaaag attaggatta
      781 gtctcagcag atgaggcaaa acaaattact gaaaaactag atccatatat taataatcct
      841 gctgtaaaaa cctggtttga tggcagttat aagattctaa acgaaaggac tattattaca
      901 gcggatgctg aaaattacag accggatcga attatgatag gcaaagacga aataattgtt
      961 gttgattata aattcggaga aaaggaagat tcttatttta gtcaggttag tacatatatg
     1021 tcattactta agtcaatggg gtataagaat gtttcaggct atatatacta tcagattgat
     1081 ttagagatag taactgtcag ttagcttttt aaattcttct gtatattcgt gtcgagtatc
     1141 agtttctatg cataattgat gctccgtcaa atcttgcttg ttttttgtta gttcaagaat
     1201 taatcgtatt accttagctc ccggtttcgg aataattttt gtgcatttcg ttacaaataa
     1261 actgtatttt tctgcttctt ttataaaaat ttctgcctca atagtaggta agataagaca
     1321 gaatcttcca ttgtctgata atatttttgc aacagatgaa caaagtttat cgaatggtaa
     1381 tatgtcatta tgtctggcaa tgctacgttc gtgctcaggt gctttaagcg attgcttgaa
     1441 atagggtggg ttgctgatta tcaaagaata ttgtttgtca caattctcag cgtattgtat
     1501 aaaatcaaca catattatgt tgatattttt ccatggtgat gccatgatat tatttcttgc
     1561 ctgattagcg gctttctcat ctatttctat tgcatcaatt gttgatgagg tagatttctg
     1621 tgcaagcatt aatgcaatta atcctgttcc ggtacctata tctaatattt gttcgtcaga
     1681 gtcaatttgt gtatacgctc ctaataacac gccatcagta ccaaccttca tggcgcaatc
     1741 agactgcata attgtaaatc gtttgaactt aaaatagtcg tttggcatcc ggattaattt
     1801 ttgatcgggc aaatatacac aaaaaagaaa acactacgcg gtacgtttac catgtagtgt
     1861 tttccaccct taaaaactta tgaaaaaatg aattatgaaa taatgttatt taacaaccat
     1921 tgtttcggtg ttgaatacag caccgttgta aatagttacg aaatatctgc cttttgcaag
     1981 atgagctata tctaaattac attcattgct attaggttta attgatatta caactgcgcc
     2041 attttgtgca cgaacttcga ttttactaat ttcttcttgt gatacgatgt taacaaattg
     2101 aactgcagga tttggagaaa tttttgcaat tgcagtgctt gtttcttgta ctgatgttgc
     2161 agggcaatct ccaactttgt aatagatagc ttcaggtttt gttaagtttt ctgctgtcat
     2221 ttcattaccc gggatgtcac ttgtacgata caagaataat gatttaacag attctcctgt
     2281 atattgggtt gtgtatgttg cagatttttc gtcattagcg attgtcatta cttgattacc
     2341 gccttcagtt tgttgaattt ctgcatacat caatgtaagc tcactgttat atggagtaac
     2401 tgtatatgtt acattaccac ttatggcatc gtatgatatt acataatgaa tacgaagaac
     2461 tgttggattg ccaaagtgtc ctttatctcc tgaacattgg gattctaatg tttcagtact
     2521 tgctattaca gattctgctc cggtagaaat attgttgcaa taatcttttg ctttaataga
     2581 aaagttatat tctgttccgg gtgttaaacc gtctattttg attacaccgt ctgtggcagt
     2641 aaatactttg tctgtgaaac ttgcgcaaga aactacgaag tttgtaatag cgtttgagtt
     2701 ttgatcaaca cctgcaacat ttaaatttat atcgtatgct gtttttgatt ccaaagaagc
     2761 agaagtcata gtcggatttt ctgttaaatc gcaatctcct gaacaatttg ctgtaacatc
     2821 aatatcagat accgatactt gccaacgacc ggcccaatca ggattaccat attgaaccat
     2881 tatacttgaa gttgtagtac cgggattaag tgtagcttca atagctaatt ctgctaaagg
     2941 ttcgcctgtt tgagctgttc caaattcgta gaaaccttgt gttgcatcag atacggtaac
     3001 atatatgtaa ttaatacctt taacgttgtc taaaccaggt tttactacaa tgtttagttt
     3061 gccggcttcg tcgcctgaaa tcgttaataa tatatgtgaa tttacatctc cgaaattcgg
     3121 gtcgttaaga tgtcctgtcg gagtatcgca ccattctgat gcaggtgctg caaaagcagc
     3181 aaatcctacc atgatcattg tcacggctga gaagaatctt tttgtaattg ttttcatgtc
     3241 tttgtaattt tataggttgt taaaattaat taggtcgatt tttacgactg tcatttgtgt
     3301 ttttgttttg aacacgtagc aaaggtattt agtttttgat tgcaatcgtt tgtcgtttta
     3361 tgcacaaaaa ctcatcacga aaaataaaaa aatacatcaa cgatacatcg ttaatgtact
     3421 aatttgttta taatcagtag tttataatta attttgttat attgcaaaaa ttaacgactt
     3481 aatgcgtaat tccccttttg tagcattttt cgtctatttt tttatgcaag tagtagaaat
     3541 tgaatgtgcc ggtaatatgc aattgtatgt tttatctctc atttgcattg aaaattctat
     3601 ttctttatct tctttattca tgcatataac tacaatactt ttgtcgggat tcatggctgc
     3661 tgtacagtat aattcggctc tgcttgatgc acatgcaatt cgaacagctc cgggtttaat
     3721 aaatttcgag aaatgtccga tgtagtaata agagtttgtg tacattagct ttcctgtttt
     3781 agtatttcca tgaattggtg cgaaacaata gttttgtaca tggttgggtc cgcctttttc
     3841 atctagtaat atgttccaat cagtccaagc tgatgtgccg caattaaaat cattaatcat
     3901 actgtatccg tagcgttctc ctatcgacca atcatttata ttgttaaggt caaatttttc
     3961 tacgcatcct tctgtaaaaa taagttcttt atcagggaat gattctttta ctctacgtaa
     4021 attttcaaag tacataggac tttttgtcca tgtttcgtac caatggaatc caactcccca
     4081 cacatatttt gctgcctcag gatcattcaa tattgttgct gctctgtgat atataagatc
     4141 tctgttatga tcccagatta tgattttttt atcgcctaat ttgttctttt ttaatgtagg
     4201 ccctaagttg ttttttatga aatcgcattc atcttgagct gtgtatatgc atgattccca
     4261 tgtttgtgta gccataggtt cgttttgtgt gctaagtccc catactttta tgccttcagc
     4321 ttcgtaggct ttaatgaatt gtacaaaaca ttctgcccat aattgtctgt attcaggaag
     4381 taatttaccg cctcttatta tactacgatt atctttcatc catagaggtg ggctccatgg
     4441 ggcaacataa aagattgaac cattacctat tgatttaata gcttttttta taagtggtat
     4501 tctatactgt ttatcatgtt cgatgctgaa tgatgataat gatatatcgt tttcttccca
     4561 gtaagaatag ctgtatggac taaaatcaca gctttgtatg ttagacctga ttatattata
     4621 agctaaaccg ttttctttat caaaatatgc tgataataac tcttcctgag cgtctttagg
     4681 aagttttgca aaaacttcgg ctgatgcatc cgtaatagca cctccaaatc caatgtattt
     4741 ttggaaatga tgtagcggat caacgtacac cgaatattta tcttcggttg gctgttcgct
     4801 tttaatcggg ctgattatgg tcttttgata tcgtgtatcg ttagccatgg ccgttgtata
     4861 taccgtagct gtttgtgctt gacaaatcat acttaatgat atacaagcag tagtaataat
     4921 taaatgtgct attctcataa atgtatctgt tattatctat aatttattta tctcttaaaa
     4981 tggcttgagt tgcgcaacgt attgatccgc cttggccatt atgagctcca aaaaagacct
     5041 tatgaactgt aattccatgc gattccaatg ctgatttaat tcgatctgat gtcacttctg
     5101 tatgtttgtt ataactcatt atataatttt tgtcgttaac cggcaagcca tttactgcaa
     5161 gtcttgatgc ttcttcaagc gatacttcta taaaatccca gccattaagt gcttcaggaa
     5221 ttccatcaga gaatgcgtct ttgcaaatta tggctaagcc ttctttagga atagaaagag
     5281 cacaatctaa gtgtaatata gtttcaacca aaggtactct tattaccttg taatcgtctc
     5341 ctaaataatt tttaagccat ttgtaaccgg cttcatttga tcctacagct ttgtttgctg
     5401 tatttccaac tagaattgtt tttcctaata ccattacatc gccaccctct aaggcaggag
     5461 tttcgtcaga tggaacaggt aaaagtggaa tttgtggcat cgaaacccaa acaaccgatt
     5521 cgtcgcattt tgttgtaagt aagtggttaa atccgattat gtcaaaacgt ctgagaggtg
     5581 ttttaagtgt tagttcaata acattattgc caattattgc cagattatca cgaggaaaat
     5641 cttgagaata tccgttaatt agggctgcct caccatagtt ttctataacc cattcttctg
     5701 taataaggtc aggtctgtat acagtaactc ccaaggattc gaatacggca ataagttcat
     5761 tgttttcaga atcaattcgt tgagcctccg aaatagtgcc ggtaggatcg tattttcctt
     5821 gatcgtcgta ttttatgtct gtccaattgt atcctgcggt tgcttctgca atgtctcttt
     5881 catcttgagg taaaatttta attgcctctt tataccatgg cgcatctata ctaggatttt
     5941 taccactagc taatcctact attacttctt ttaaatttcc atattcacta tttatataga
     6001 tcttacggct gcctggagta ggttgatcgt ggcaagaact caaagttgct aatgacagca
     6061 ataaaatgat tgacgatgct attgatttca taatcattga attaattttc caaagttaaa
     6121 tatttttttg aaaaataaat tgatgtagta gtaaaagaaa ggaaaaaagt ggatgactaa
     6181 taaggattag agtaggtggt atcgatttca aaacaatcct atatgtttta catcaaccaa
     6241 ttaaacaacc atccgctaat gataattaat aaggccgtta ctccaaagaa tatgcctatt
     6301 agtttccatg tcatcactgt ttttagaagc attgcctccg gaaaagaaag ccctatgaca
     6361 gccatcatga aagcgatggc agttcccatg gatatgcctt tttgaacaag tacctctaca
     6421 attggtacaa ctcctgcggc atttgcataa ataggaacgg ctacgattac agctaatggt
     6481 acactatacc atttgtctga agacaaaata ctctcaaaaa aaccgtcagg tacaaatccg
     6541 tgcatggcag cgcccaaacc aatgcctata atgatataga taaacacgct tctcacaatt
     6601 ctccaagatc ccaatactat gtttggaagt cttttccaaa aggatatttt ctctgattcc
     6661 cattcttcag tttcttccac actcttcttt tgaagttcga gcacccatgg actcaatagt
     6721 cgttctaaat gaaaacgttc taatattaat ccgcctatca tgctcaatat taacccgcta
     6781 actacataaa taatagttat ttttagtcca aaagttccca aaaacatagc tattgctatc
     6841 tcacttaaaa gaggagaaga tattagaaat gtcatagtaa cacccatagg aattcctcct
     6901 tttacaaacc ctataaatag aggtacgctg ctgcaagagc agaatggtgt gatggcgcct
     6961 agtgtcgcag ccaagaaata ttgtaatcca aacattttgt gtttagttaa atagttgcgc
     7021 acgcgatcta tcggaaaata ggcatttatt attcccatca aaacactaat caagaacaga
     7081 agaatcaata ttttaatggt atcataaaag aaaaaattga cagctgttcc taaactgctt
     7141 ccttctttta aaccgaaaac aacatatact aaccaatcag caaaattttg aatcatatta
     7201 taaatatata gtaacaaaat aataaatacc aatagtaatg cagagaatgc ctactactat
     7261 gttgaaccat ttttgaaaaa caagcatttt gccatagaat ttgccgatac tattagcact
     7321 atatgctata atccaagcaa caataataac cggtagagct gttgcaaatg caaatactgc
     7381 aggtaaaaaa tatccacccg aacttgtcgc agaaagggga atcagcatac caaaataaaa
     7441 aacaccgcta ctcggacaaa atgccaaagc aaataacatt ccgagaaata gtgcacccca
     7501 accacctctg tttttcaaac tttcactgtt gcctttaaat ccgaatgatg ggagtttaat
     7561 tttgtctgaa aacaaaatga aaagtcccaa caatagcaag ataaacggaa gaaataactc
     7621 gccgtattta ctaactgttt tttgaatatg aaacgtactt gcaccttctc gtaaaatata
     7681 tatcagtatt gcgccaagta aggtgtaggc aataattttc cccaatgtgt atagcaaacc
     7741 atttagtaaa acacgttttt tactagctat atccttactg atatatccaa tggcggtaat
     7801 atttgtcgcc aatgggcaag gacttacggc agtgagcaat cccaataaaa aagcaatgag
     7861 aataggaatg ttgctgctgt caataatttg ctaaatccat tccattactt aaggatttta
     7921 tacaattcgt cctttaactt tgctttgaat acatccggtt gatttttggc ataactaaat
     7981 cccatatcgg taagatttac agtttttcct tttttgtcta atattaatga cgaccatgca
     8041 acctcgtact tgtcggctag ttcttcgttt tctgtaatgt caattacttt ataaactatt
     8101 ttattgttat tcagttcctt tttaaaatct ttttcaagaa cctctttcgt aagcgattct
     8161 attgacacac aagttatgca gcgttgtttt ccatggaaat acaatacttc aacagtattt
     8221 gattttattt tttttgtttg tttttttgtt tctgcgtttc cgcatgaaac aaacattgtt
     8281 aacgcgatta ttaaatatat aagacgtttc attttttacc ctcctgtaat tcttttaata
     8341 atgattttat ttccgacaaa gaaagtcttc ccttagctac gactttttcg tcaactacaa
     8401 tagcaggtaa cgacattacg ttatactgca tgattttcat caagtcttct tcttttatta
     8461 tttctgcttg aatgttcatt tcagatacgg ctttttgaac agtttcgaat agggccttgc
     8521 atccagcgca gccggttccg agaattttaa ttttcatttt actatatgtt ttatttgtaa
     8581 atattatttg tagttcgcct aacaacgaac attaaaatca aaaaaaacta tttgcagcag
     8641 cctccttttt tgcagacgca gtcatcaaat agttctacga aaagcgattt agctaatttc
     8701 caattgtcac gattgataca atattttact tttggggctt ctatttcgcc ttgtattagt
     8761 cctgcatctt ttaattcctt tagatgttgc gatacagttg cttttgcaat tggaagttct
     8821 tcaaaaatat cgccaaaata gcaagtagta cgattatcca aaaatcgtag tattgcaatg
     8881 cgtgttgggt gacccaaggc tttcgcaaaa cgtgctatct gttcgttatt gagttctttt
     8941 gtcatatatc cacgaataaa ttgttcgcaa tattacgaac aatattttaa ttggcaatga
     9001 ttttaaaaat attttacaat aaaccccaag tatttatttt taatactcgg ggttttgcta
     9061 tgttttaata ttattgattt gcagtttttg cttttaattc ttcgtagaaa tgactgtgtg
     9121 atgtatatat gtaaggtaac aaccacaaga aaccgatacc caatgagagt atgcttagta
     9181 accaccatcc tataaagcta agatctagca agaaaagttt cattttgtaa ccattcatca
     9241 tacgcatact ttcatttatg cattcttcag ctcccaattc gggtttatct aaggcaataa
     9301 aaaatgcttg agaataagag tatgatttaa taatacccgg gacaaacaac aatagagtcc
     9361 acaaaataat atatatggct tgaagtaaag atacacttaa tgctctgccg taatttttaa
     9421 agcaatcaaa cattctagca atcaaattat ctttatcgcc tcgtataaat atcaaatatg
     9481 cctgagtcaa actataacaa acaggtacga aaaagaatat tgatactata attccgccaa
     9541 aagggattgc ggtcattgca ctcattaaga gtataagaaa agttgataac acaggcatag
     9601 accaattgcc ttttaatgaa tcgcaagcca tgctgcgaat ttcctaattt gttttcataa
     9661 tgctttaatt ttatggtaaa tcacaaattt atcattaaat atttaaaata tcaatatttt
     9721 gtggcatttt tttgataaaa taccattaca ggcatcttcc aaaatatcga gtacgtactc
     9781 aaaacctccg gctcctccat agtatggatc cggaacatgg tctgcaatta tattgacgca
     9841 ataatcggtc attcgactta ttttgttttt tgtttctaac gttggtgcta attcttgcaa
     9901 tgcatcaata ttgccatcgt ccattccaag tatcaggtca aaattataaa agtcgtctat
     9961 agtgatgcgt cttgctctgt gcgtaagatt gtatccgcgt ttcatggcgt gggtgcgcat
    10021 tcgcgaatcg ggtaattcgc cttgatgata atctataatc ccggccgaat caatctcaaa
    10081 tagttcggag catcctttct ctttaattaa atgcagcata ataccctcag cagcaggcga
    10141 acggcatata tttccaaggc acacaaatag aatttttgtt gtcatgctca aagttaatta
    10201 ttttttttga ataagagttc gcttaaactc atgcaacata caaagatttt gccacctgaa
    10261 cgaaagctat tcccatattt aatggctgtt aagagattta aatagggggc ttttcgtatc
    10321 tttgcagctt caaaaattag atgtatggat attcttaaaa tttcagaaca aaatcaaaag
    10381 actgcttgga aaatacttga agatactcgt attattaagg catgggagag tattggagca
    10441 actgttaata ttataggttc gcttaaaacc ggcttaatga tgaaagcttg tgatatagat
    10501 atgcacattt ataccgataa actggatata gctgagagtt tttctgttat gcaaagtttg
    10561 gcggaaaaat tacaattcaa agaaatccac tatataaacg gaattgatac tgaagaagaa
    10621 tgtatagagt ggcacgtttt gtatgaaaac aacgatatga atgtttggaa aatggatatg
    10681 attcaaattc gtaaaggttc caaatacgat ggcacggtag aaaatgttac aaatgctata
    10741 atcaataaac taactccaga tattcgtcaa acaattcttc aaatcaagta tgatattcct
    10801 gataacatta agattcctgg aatagagata tatcatgcag tattttcagg gaaagtgaaa
    10861 acttatgaag aacttgaaca atggcgcaaa acaaatccat tgacaaatag tttggactgg
    10921 ttgccgtaat tattaaataa tgtcgtaata tcgtgactga ttaatgtata tacaacagtg
    10981 cagatactct taaa
//
LOCUS       DAOPID010000028        10649 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_7597_length_10649_cov_4.390787, whole genome shotgun sequence.
ACCESSION   DAOPID010000028 DAOPID010000000
VERSION     DAOPID010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 10649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10649
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7597_length_10649_cov_4.390787"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PK641_01985"
     CDS             <1..262
                     /locus_tag="PK641_01985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPG55158.1"
                     /translation="AAHHTPAILLEKTTSAEVIESGKNGFLSPQGQPSVFADTIKKVL
                     SDKKNLDNVGKTASETLGLRWSQLMPEVEDRYRHLILRKQND"
     gene            255..1349
                     /locus_tag="PK641_01990"
     CDS             255..1349
                     /locus_tag="PK641_01990"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPG55159.1"
                     /translation="MTNQTTEASVSKRLKPSHIILPILFGFAGIVWMFCSEFSEIKDA
                     EIPFEKYLNGYTVLFLFLALLAVAIRDLSGMGRLYLLSEKQLSFHSLFRIRMLYEFTS
                     AITPSAAGGSTLEMLFIHKEGVKFGKSTAITIISLFFDELFFVIGFPILLLILPIDLL
                     FAVEGTFSAAILSVFAIAYLSKVAWLVVLFIGIFIKPSTISWIIQKVFSLRILKRWRI
                     GAYRTAIDVKECSRNMRHKSIVFWLKNLGYSVLMWVSRFTVVNFLIMAFNQSTESLMS
                     FTTQFLILGRQLVLMVVMLVAPTPGASGFIEVLFNSYLGEFIPKGIVLLLITAIWRIL
                     IYYNYLLIGAVLAPRWINRNYSKKNKSVTK"
     gene            complement(1353..3890)
                     /locus_tag="PK641_01995"
     CDS             complement(1353..3890)
                     /locus_tag="PK641_01995"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HPG55160.1"
                     /translation="MKRICLFLLVFVLCNSINFCYANTYKGTVTDSITGEPLPFISIR
                     IANTLYGDETDIDGSFSIQAEPENQLIFSFLGYENKTISLKKQTGKSIQIKLVPTDFK
                     LAEVEITAKNSYSKKNNPAVDLIRRIIAEKNENRIENAAYYSRKTYEKTVLGITDLST
                     KSTNALGIGDVVKNSDTSIVSGKKILLLSLKEKTMDQYYRKTPKTKKQIITGRQEKGI
                     DDDVFAEGWLTTYMDELFKDVDIYNSNILIAFKPFVGPLSPIAPDFYKFFIKDTVYID
                     GIKCINLGFTPFNSANFGFMGYVSVDDSTLAVKRVSMDIPPSTNINYIDGLHIEQHFE
                     KNEQGTMELIDDQLTAEFYIVKGTKGFYSQRTRNFSDYTFNEPNDSIMRSFSGTELIL
                     KDAVKQDSTFWNAHRTDTLNINESKMTVLLDKLKKNPVIRVIMFGAELLIKGYLRTGK
                     PSAFDIGPVYAMFSSSAIEGFRLRIGGESTTNLMPRLFTSGYLAFGFGDNKIKYNGTL
                     EYSFIDKSYHAREFPVNSITISATSDIRTLGESPAATLSDNLFYSIKRMQVTNMMYYK
                     QAKLQYMYEDLSGFSINPWILYEQREGAGSIDFKQRISKDLETPKYKDIPYLYHNEIG
                     LTLRYAPREKFVQGRIYRFNLKNPYPVFELTHRASFQNVLGSQYGYQATEFKYLQRFF
                     LSAFGRIDLTFKGGKIWTGGVAYPDLFCPNSNTSFTIMQESFSQVNPLEFVADQYLSI
                     DIGISTNGLLFNLIPGIKKLQLREVAGFKCYWGHLSNKNNPMYNSGLLAFPGNTGTLT
                     MEPYMEFSVGIDNIFRILRVDYVRRINYLQNPNIWANGVRVSLNFTF"
     gene            4113..4640
                     /locus_tag="PK641_02000"
     CDS             4113..4640
                     /locus_tag="PK641_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPG55161.1"
                     /translation="MPDEYFPIVDKNGLVIGKETRKYCHSGSFLLHPVVHLHIFDSKG
                     RLYLQKRAITKDIQPGKWDTSVGGHVDYGETINEALIREVREELNIVDFNVQKMFSYT
                     FRSTIEYELVNSFYIIYEGLIEPDPTEICEGRFWTIEEIESKLGMSIFTPNFELEFAK
                     IKANGFINVITNKND"
     gene            4644..5162
                     /locus_tag="PK641_02005"
     CDS             4644..5162
                     /locus_tag="PK641_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55162.1"
                     /translation="MKRLFLIAFSALFCLSINAQEMSLDEYAQNTNKKKEATRMSFND
                     KDFDAKTMASQESMRFKKNLMLTDKQSERVYKYLQKEFQKFEDRKPRVVYSGGMGGPG
                     MASPGGLSDNMVMPPLDGGRPSISGGEGSKSKQRTSETEIKRLKEKCAKQYKKIFTPE
                     QYSKWEEIEKER"
     gene            complement(5229..6242)
                     /locus_tag="PK641_02010"
     CDS             complement(5229..6242)
                     /locus_tag="PK641_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGGtGRT protein"
                     /protein_id="HPG55163.1"
                     /translation="MAIREVKFESKDRRIKGVIAALNANGIKDIEEANVICDAAGIDP
                     YKTCEETQPICFENAKWAYVVGAAIAIKKGCKNAAEAAEAIGIGLQSFCIPGSVADDR
                     KVGIGHGNLAARLLREETKCFAFLAGHESFAAAEGAIKIAAKADKVRKEPLRCILNGL
                     GKDAAQIISRINGFTYVQTQFDYYSGELTVVREIAYSDGARAKVRCYGADDVREGVAI
                     MWKEGVDVSITGNSTNPTRFQHPVAGTYKKERILAGKSYFSVASGGGTGRTLHPDNMA
                     AGPASYGMTDTLGRMHSDAQFAGSSSVPAHVEMMGFLGIGNNPMVGCTVAVAVDIATA
                     LNK"
     gene            complement(6255..6956)
                     /locus_tag="PK641_02015"
     CDS             complement(6255..6956)
                     /locus_tag="PK641_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55164.1"
                     /translation="MVYSHEVEHMCVVKKGPNHGPAPIPEEGKWVKAKEVKDISGLTH
                     GIGWCAPQQGACKLTLNVKDGVIQEALIETIGCSGMTHSAAMASEILPGKTILEALNT
                     DLVCDAINTAMRELFLQIVYGRTQSAFSEGGLIIGAGLEDLGKGLRSQIGTLYGTLAK
                     GTRYLEMAEGYIKTLALDKNDEVCGYEFVHMGKFMDEIKKGTDANEALKKVTGTYGRF
                     TKEQGAVKHIDPRKE"
     gene            complement(7037..8968)
                     /locus_tag="PK641_02020"
     CDS             complement(7037..8968)
                     /locus_tag="PK641_02020"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG55165.1"
                     /translation="MKKLLYVLAFIFTATTSMAQLNTQRLLDIGRNALYFEDYVVAIQ
                     YFNQVIKVKPYLTDPYLYRAYAKLNLEDYRGSISDCNKALEINPFLPKVYYCRGYAYR
                     QINMLEASTDDFKASLKLEPDNINTTNLLIENLIRQKLLDEALSQCDSFLIKYPRYTE
                     TYLLRSQIYLEREDTIKASGDLDTAISISSENDLAHAFRAMLRYEQKKYAESIEDMND
                     AIRINAFRSDYFGNRAIIRMQTKDLRGAMNDFDTAIEIDNKNASVYFNRGILRSQVGD
                     DNYAIDDFTKCIALDPSNYTAYLQRAYLRSTIGDLKGSVSDFTKVIERYPDFVVAYYG
                     RSEVKKKMRDKLGAERDYYTALNIENEIKSGKRKQKTGKEAEELPTAESRAIVQELNK
                     KQNDRYKSDIRGQVQYKDIDIEPLSNFVIGIPYSDTEIQRKTLFNKQLDDYNKRQGRK
                     LQFCINEKEVDSAMLEEYFKAIDNANNILSKDSLNETALMERAYCLSKVQDYASALKD
                     FDRILPYHPNNVITYFNRGNARLKAIKENNYDNDIPLAEYTMAVSDFMMAAKIDPTFI
                     YAIYNAGYVYMQEKSFQKAIECYNNAINIYPEFAEAYYNRGLIYLFQGDKVKGRADLS
                     KAGELGIHGAYNVIRRYAY"
     gene            complement(8965..>10649)
                     /gene="lon"
                     /locus_tag="PK641_02025"
     CDS             complement(8965..>10649)
                     /gene="lon"
                     /locus_tag="PK641_02025"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPG55166.1"
                     /translation="IQDKAMENIDKQQREYFLQQQLKTIQNELGGSVQEKDMSLIRKK
                     AETKKWDEKTKKIVLKEIDKMESSSPHSADYPVQMNYVQTLVDLPWGEYTKDNFNLKR
                     VQRILDRDHFGLDDVKDRIIEHLAVLKLKGNLKSPIICLYGPPGVGKTSLGKSVAEAL
                     GRKYARISLGGLRDEAEIRGHRRTYIGAMPGRIIQNVLKANSSNPVFILDEIDKVGND
                     YKGDPASALLEVLDPEQNSTFHDNFIDIDYDLSKILFIATANDLNTISPALLDRMELI
                     NISGYITEEKIEIASKHLIPKEWDNHGIPAKKVSISKKAIEAIIENYTRESGVRNLDK
                     QIAKIARKIAKKIASEEKVDSIIGENDLHEYLGTAPYDRDKQDNKEEIGIVTGLAWTS
                     VGGEILEIETSLSKSKGEKLTLTGNLGDVMKESATLGLAYIKAHASELGINEDFFENT
                     QIHIHVPEGAIPKDGPSAGVTMATSLLSAITGKRVRKHIAMTGEITLHGKVLPVGGIK
                     EKILAAKRAGVTDIIMCKDNKKNIEEIKDIYVHGLKFHYVKNIQEVFELALI"
BASE COUNT         3118 a         1851 c         1876 g         3804 t
ORIGIN      
        1 tgctgcacat catacgcctg caatcttact tgagaagact actagtgcag aagttattga
       61 gagtgggaaa aacggtttct tatctccaca aggtcaacca tccgtttttg cagatacgat
      121 taaaaaagtt ttatctgata aaaaaaatct ggataatgta ggtaaaacag cttcagaaac
      181 attgggatta aggtggtcac agttgatgcc tgaggttgaa gataggtatc gtcatttaat
      241 attaagaaaa caaaatgact aatcaaacta cagaagcatc tgtttcaaaa cgattgaagc
      301 catcacatat tattctgcca attctgtttg gatttgctgg gattgtatgg atgttttgct
      361 ctgagtttag tgaaataaag gatgcagaaa taccatttga aaagtattta aatggataca
      421 cagtgttatt tttattcttg gctttattgg cagttgctat tcgagattta tcaggaatgg
      481 gacgtttgta tcttttgtca gaaaaacaat tatcattcca ttctttgttt agaataagaa
      541 tgttgtatga gtttacttct gctataactc catcggctgc aggaggctct actttggaaa
      601 tgttgtttat acataaagag ggagtgaaat ttgggaaaag tactgccatt acaattatat
      661 cattattctt tgatgaattg ttttttgtaa taggattccc tatcttgttg ttaatattac
      721 caattgattt gttgttcgcg gtagaaggca ctttttctgc agcaatattg tctgtttttg
      781 ctatagcata tctatctaaa gtagcatggt tggtggtttt attcattggt atttttatca
      841 aaccatctac gatttcttgg ataattcaga aggttttttc tttgcgtatc ttaaaaagat
      901 ggagaatagg ggcatataga actgcaattg atgtaaaaga atgctcgcgt aatatgaggc
      961 ataaatcaat cgtcttttgg ttgaaaaatc taggctatag cgttttgatg tgggtgtctc
     1021 gttttacagt agtaaacttt ttgataatgg catttaatca atcgaccgaa tctttgatga
     1081 gttttacaac gcaattttta attttaggtc gtcaattagt tttgatggtt gtaatgctag
     1141 ttgctccaac tcctggtgct agtggattta tagaggtact atttaattct tatttgggtg
     1201 agtttatccc aaagggtatt gttttattgc ttatcacagc aatatggcgt atattaattt
     1261 actataatta tttacttatt ggtgctgttt tggctcctag gtggattaat agaaattact
     1321 ccaaaaaaaa taagagcgtg actaaataaa tcttagaagg taaagtttag agatactctt
     1381 acaccgtttg cccagatatt gggattctgt aaatagttta ttcgtcttac gtaatctact
     1441 cgcaatattc tgaatatatt gtcaatacct acgctaaact ccatataggg ttccattgta
     1501 agtgttccgg tgtttcccgg aaatgctaac aaccctgagt tatacatcgg attgttttta
     1561 tttgacaaat gtccccaata acacttaaat ccggctactt cgcgtaactg tagtttcttt
     1621 attccgggta ttaggttgaa taataagcca ttagtgctaa ttccaatatc gattgacaaa
     1681 tattggtctg caacaaattc aagaggatta acttgactaa atgactcttg cattattgta
     1741 aatgatgtat ttgagttagg acaaaatagg tctgggtatg ctacaccgcc ggtccatatt
     1801 ttacctcctt taaatgttaa gtcgatacga ccaaatgccg ataaaaagaa acgttgaaga
     1861 tatttgaatt ctgttgcttg atagccatat tgtgaaccaa gtacattctg aaaactagct
     1921 ctgtgagtta attcgaatac aggataagga tttttaagat taaaacgata aattctacct
     1981 tgaacaaatt tctcacgtgg ggcatatcgc aatgttaaac ctatttcatt gtgatataag
     2041 taaggtatat ctttgtattt aggtgtttca aggtccttcg aaatgcgctg tttaaaatca
     2101 atacttccgg ccccttcacg ctgctcatat aatatccatg gattaattga gaatccactt
     2161 aagtcttcgt acatatactg taatttggct tgcttatagt acatcatatt agtaacttgc
     2221 attcgcttta tagaatagaa caagttatca gataatgttg ctgcaggaga ttctcccaat
     2281 gtacgtatat ctgaagtggc tgaaattgta attgaattaa caggaaattc tctagcatga
     2341 taacttttat caataaatga atactctaat gtaccattgt attttatttt gttatctccg
     2401 aatccgaaag ccaaataacc acttgtaaat agacgaggca ttaggtttgt tgttgactcg
     2461 cctccaatgc gaagtctaaa accttcaata gcacttgaac tgaacattgc atatacagga
     2521 cctatatcaa atgcgcttgg tttgcctgta cgtagataac cttttattaa tagctcagca
     2581 ccgaacataa tgactcgtat aactggattc ttctttaact tatctaacaa aacagtcatt
     2641 ttactctcgt ttatattaag agtgtctgtt ctgtgtgcat tccagaatgt gctatcttgt
     2701 tttactgcat ctttaagtat aagttcagtt cctgaaaagc tgcgcattat tgaatcatta
     2761 ggctcattga atgtatagtc ggaaaaattt cgcgtgcgct gtgagtagaa tcctttagta
     2821 ccctttacta tataaaattc agcagtaagt tggtcatcaa ttaattccat cgtaccttgt
     2881 tcattctttt caaaatgttg ttcaatatgt aaaccatcaa tataatttat gtttgtacta
     2941 ggtggaatat ccatacttac gcgttttacc gctaatgtag aatcgtctac tgatacatat
     3001 cccataaatc cgaaattagc agaattaaat ggggtaaatc ctaagtttat gcattttata
     3061 ccatcaatgt atactgtgtc cttgataaaa aatttgtaaa aatcaggagc tataggtgat
     3121 agaggcccaa caaatggctt aaatgcgatt aatatgttgc tattatatat atcaacatct
     3181 ttgaatagct catccatata ggttgtaagc catccttcag caaatacatc atcatcaata
     3241 cctttttctt gtctacccgt aataatttgt ttcttggttt tcggtgtttt acgatagtat
     3301 tggtccatgg ttttttcttt tagcgaaaga agtagaattt tctttccgct aactatggac
     3361 gtatctgagt tttttacaac atctccaatg cctagtgcgt ttgtactttt ggttgaaagg
     3421 tcggttattc ctaatactgt cttttcatat gttttacgag agtagtatgc agcgttttct
     3481 atacgatttt cgtttttttc agcaataatt cttcgaatta agtctactgc aggattattc
     3541 ttttttgaat aagaattttt tgcggtaatt tcaacttcag ctagtttaaa atcggtaggt
     3601 accaatttaa tctgtatcga ttttcctgtt tgttttttga gggatattgt tttgttttcg
     3661 tatcctaaga atgaaaaaat caattgattt tccggttcag cttgtattga aaatgagcca
     3721 tcaatatctg tttcatctcc atatagggtg ttcgctatac gtatagagat gaatgggaga
     3781 ggctcacccg tgatggaatc agttacagtg cctttgtatg tatttgcata acaaaagttg
     3841 atgctgttac aaagcacaaa tactagtaag aataaacaaa tccttttcaa aataggatga
     3901 ttggttgctc gttggttttt aaggcgcaaa ggtaataaaa tttgcttaaa acattatatt
     3961 ttaaattgct aaaagtcttg aattgtttac ctttgtatca atttcgtttg tattaatatg
     4021 taattacaag agtattaata atgctcatat ttggaataat ctaaaattac atatcggatt
     4081 gatgaatata aaatttatct aaaagttaaa taatgccaga tgagtatttt ccaatagtag
     4141 acaaaaatgg gcttgtaatt ggcaaagaaa cacgtaaata ttgccatagt ggaagctttt
     4201 tacttcatcc ggtagttcat cttcatattt ttgacagtaa aggtagattg tatttgcaaa
     4261 aaagagctat tacaaaagat attcaacccg gcaaatggga tacatcagta ggagggcatg
     4321 ttgattatgg tgaaaccatt aatgaggctt tgattagaga agtgcgagag gaattaaata
     4381 tcgttgattt caatgttcaa aaaatgttct catatacttt tcgttctaca atagaatatg
     4441 aattggttaa cagtttttat atcatttatg agggattgat tgaaccggat ccgaccgaaa
     4501 tatgtgaagg tagattttgg acaatagaag aaattgaatc aaaattaggc atgtctattt
     4561 ttaccccaaa ctttgaactt gagtttgcta aaattaaggc taatggattc attaatgtaa
     4621 taacaaacaa aaatgattaa tttatgaaaa ggctgttttt aattgctttt agtgcgctat
     4681 tttgtttatc cataaatgct caggaaatga gtttggatga gtatgcgcaa aatactaata
     4741 agaagaaaga ggctacgcga atgtctttta atgataagga ttttgatgcg aaaactatgg
     4801 catcgcaaga atcaatgcgt tttaagaaaa atcttatgtt gacagataag caatcagaaa
     4861 gagtttataa atatctacag aaggaattcc aaaaatttga agatagaaaa cctagagtag
     4921 tatatagtgg aggtatgggt ggacccggta tggcatctcc cggagggctt agtgataata
     4981 tggtaatgcc accactagat ggaggaagac cttcaatttc gggaggagag ggatctaaat
     5041 cgaagcaaag aacatcagag acagaaatta aaagattaaa agagaaatgt gctaagcaat
     5101 ataagaaaat atttactccc gagcagtatt caaaatggga agaaatagaa aaggaaagat
     5161 gatttaatgg caataaaaag actctcactt gttttagtga gagtcttttt ctattataag
     5221 attaattctt atttatttaa agcagttgca atatctactg caacagccac agtacaacct
     5281 accatagggt tgttaccaat tccaaggaat cccatcattt ctacgtgtgc aggaacagat
     5341 gaagaaccgg cgaattgagc atcagagtgc atacgtccta gtgtatctgt cataccataa
     5401 gatgcaggtc ctgctgccat attatcaggg tgaagtgtac gacctgtacc accacctgaa
     5461 gctactgaga aatatgattt accggctaag atacgttctt tcttatatgt gcctgctaca
     5521 ggatgttgga aacgtgtagg attggttgag tttcctgtta tagaaacgtc aacaccttct
     5581 ttccacataa tagcaacacc ttcgcgaaca tcgtcagcac catagcatct tacttttgcg
     5641 cgtgcaccat cagaatatgc aatttcacgt acgactgtta actctcctga ataataatca
     5701 aattgagttt gaacgtatgt aaatccattg attcttgaga tgatttgtgc agcatctttt
     5761 cctaatccgt ttaagataca acgtagtggt tctttacgaa ctttgtcagc tttagctgca
     5821 attttgatag ctccttcagc tgctgcaaaa gactcgtgtc ctgctaaaaa cgcaaaacat
     5881 tttgtttctt cacgtaataa gcgagctgca aggttaccat gtccgatacc tactttacgg
     5941 tcgtcagcaa ctgaaccagg aatgcaaaat gattgcaaac ctataccgat tgcttctgct
     6001 gcttctgctg catttttgca tccttttttg atagcgattg ctgcaccaac aacgtatgcc
     6061 cattttgcat tctcaaagca aataggttgt gtttcttcac atgttttata tggatcgatt
     6121 ccggctgcat cgcatataac gtttgcttct tcgatatctt taatgccgtt tgcatttaaa
     6181 gcagcaatta ctcccttaat gcgacggtct ttactttcaa attttacttc tcttattgcc
     6241 attgtctttc ctccttattc tttacgtggg tcaatgtgtt taactgcacc ttgttcttta
     6301 gtgaagcggc cgtaggttcc tgttactttt ttcaaagctt cgttagcgtc tgttcccttc
     6361 ttgatttcgt ccatgaattt gcccatgtgt acgaattcgt agccacatac ttcgtcgttt
     6421 ttgtcaagag ctaaggtttt gatatatcct tctgccattt ccaaataacg agttcctttt
     6481 gctaatgttc cataaagagt acctatttgt gaacgtaagc ctttgccaag atcttccaaa
     6541 cctgcgccaa taatcaaacc accttcagag aaagcagatt gagtacgacc ataaactatt
     6601 tgtaagaaca attcacgcat tgcagtattg atagcatcac aaaccaagtc tgtgtttaat
     6661 gcttccaaaa tagtttttcc gggaagaatt tctgatgcca tagcagcaga gtgagtcata
     6721 cctgaacaac cgatggtctc aattaatgct tcttgaataa cgccgtcttt aacgtttaat
     6781 gttagtttac atgcaccttg ttgaggagca caccaaccga taccgtgtgt taagcctgaa
     6841 atatctttta cctctttggc ttttacccat ttaccttctt ccgggatggg agctggacca
     6901 tggttaggtc ccttctttac aacacacatg tgttctactt catgtgaata aaccatattt
     6961 cttttatatt atattgttaa aaaaactctt ttaagccaac aaaagtagca agaaaatcgg
     7021 aatcggcaaa atgtttttag tatgcgtatc tccttattac attgtacgct ccgtgtattc
     7081 ctaattctcc tgcttttgac aagtcagccc gaccttttac cttgtctccc tggaataaat
     7141 atattaggcc tctattgtag tatgcttctg caaattcggg gtaaatgttt atagcattat
     7201 tatagcattc aatagctttt tgaaaacttt tttcttgcat atatacatat ccggcattat
     7261 atatggcata aatgaatgtc ggatcaattt ttgcagccat cataaaatcg cttactgcca
     7321 ttgtatattc ggcaagaggg atatcgttgt catagttatt ttctttgatt gcttttaatc
     7381 tggcattgcc tcgattaaaa taagtaataa cattatttgg atgatatggt aaaattctat
     7441 caaaatcttt tagtgcgctt gcgtaatctt gcactttaga taggcaatag gcgcgttcca
     7501 tgagcgctgt ctcattaaga ctatctttgc ttagtatgtt attggcatta tcgattgcct
     7561 taaaatattc ttcgagcata gcagaatcaa cttctttttc attaatacaa aactgtaatt
     7621 ttctgccttg acgtttattg taatcatcta attgcttgtt gaataatgtt ttacgctgaa
     7681 tttctgtatc tgaatatggt atgcctataa cgaaattgct taatggttct atatcaatat
     7741 ctttatactg aacttgtccg cgaatatcgc ttttgtaacg gtcattttgt tttttattca
     7801 attcttgtac aattgcgcga ctttctgcag tcggtagctc ctcagcttct ttaccggtct
     7861 tctgttttct ttttccgctc tttatttcat tttcgatgtt taatgcagtg taataatctc
     7921 gttctgcacc tagtttatca cgcattttct tttttacttc gctacgtcca tagtatgcaa
     7981 ctacaaaatc ggggtaacgt tctataactt tagtaaaatc tgatacagat cctttcaaat
     8041 cgcctattgt actgcgcaaa tatgctcttt gtaagtaagc tgtgtagtta ctaggatcaa
     8101 gtgcaataca ttttgtaaag tcatcaattg cataattatc atcaccaact tgcgagcgca
     8161 gtatgcctct attaaaataa acagaagcat ttttattatc aatttcgatg gctgtatcaa
     8221 aatcattcat agctcctcgc aaatcctttg tttgcatgcg tattatagct cgatttccaa
     8281 aatagtcgga cctaaatgca ttaatacgta ttgcatcatt catatcttcg atactttctg
     8341 catatttctt ttgctcgtag cgtagcattg ctctaaatgc atgtgccaga tcattttccg
     8401 aacttatgct gatagctgtg tctaaatctc ctgatgcttt aattgtgtct tccctctcta
     8461 aatagatttg agatcttaat aaataggttt cggtgtatct tggatatttt atcaaaaaac
     8521 tatcgcattg agatagagct tcgtccaata gtttttgacg aatcaggttc tcaataagca
     8581 ggttggttgt atttatgttg tcgggctcaa gtttaaggga tgccttaaaa tcgtcggtcg
     8641 aagcttcaag catatttatc tgacgatacg catatcctct acaataatat actttaggta
     8701 aaaaaggatt tatctccaat gctttattgc agtctgatat cgatcctcga tagtcttcta
     8761 agtttaattt agcataggct ctgtataaat aaggatctgt taggtagggc tttaccttaa
     8821 ttacctgatt aaaatattgt attgctacaa catagtcttc aaaatataaa gcatttctac
     8881 ctatgtcaag taggcgttgc gtgttaagct gagccatgct tgtggtagct gtaaatataa
     8941 atgctaatac gtatagtagt tttttcatat taaagcgagt tcaaagactt cttgtatgtt
     9001 tttaacgtag tgaaatttaa gtccatgtac gtatatgtct ttgatttctt caatattttt
     9061 tttattatcc ttacacatga taatatcggt tacacctgca cgcttagctg ctaaaatctt
     9121 ttcttttata ccaccaacgg gtaaaacttt tccgtgtaaa gtaatctctc ctgtcatagc
     9181 aatatgcttg cgaacgcgtt tgcctgttat tgcagatagt aaacttgtag ccatagttac
     9241 tcctgctgat ggtccatctt ttggaatggc accttcaggt acatgtatat gtatttgagt
     9301 gttctcaaag aaatcttcat ttattcctaa ttcggaagcg tgagctttaa tataagccaa
     9361 tccaagagta gcagattctt tcattacatc tcccagatta cctgttaatg ttagtttttc
     9421 tcctttactt ttactcaaac tggtttctat ttctagaatt tcgcctccaa ccgatgtcca
     9481 tgctaaacct gttacgatac cgatttcttc tttattatct tgtttatctc tgtcgtaagg
     9541 agctgtacct aagtattcgt gtaggtcatt ttctccaatt atagaatcta ctttttcttc
     9601 tgaagctatc ttctttgcta tttttctggc aattttagct atttgtttat ctaaattacg
     9661 aactccggat tcgcgtgtgt aattctcaat aatagcttcg atagcttttt tacttataga
     9721 tacttttttt gcagggattc catgattatc ccattcttta ggaataagat gcttacttgc
     9781 gatttctatt ttctcttcgg tgatgtaacc acttatattg attaattcca ttctgtctaa
     9841 caaagcaggc gaaatggtat tcaagtcgtt agcagttgct atgaatagaa ttttagataa
     9901 atcgtaatca atatctataa aattatcatg aaatgtgcta ttctgttcgg ggtcaagaac
     9961 ctcaagtaaa gctgatgcag gatcgccttt gtaatcattg ccaactttgt caatttcatc
    10021 taatatgaat acggggtttg acgaattagc tttcaggaca ttttgaatta tacgacccgg
    10081 cattgctcct atataagtgc gtctatgtcc acgtatttca gcttcatctc tcaaacctcc
    10141 aagtgatata cgtgcatatt ttcggcctaa tgcttcggct acagattttc ctaaagaggt
    10201 ttttccaact ccgggagggc catataaaca aattattggc gattttaaat taccttttag
    10261 ttttaaaacg gctaaatgct caataatgcg atcttttaca tcatcaagtc caaaatgatc
    10321 tctatctaaa atgcgttgta cacgctttaa attaaagtta tcttttgtat attcacccca
    10381 aggcaaatca actaaagttt gcacatagtt catttgtaca ggataatctg cagagtgagg
    10441 tgatgaagat tccattttgt ctatttcttt caaaacaatt ttttttgtct tttcatccca
    10501 tttttttgtt tctgcttttt tgcgaatcaa gctcatgtct ttttcttgta cagagccgcc
    10561 taattcgttt tgtatagtct ttaattgctg ctgtaaaaaa tattcgcgtt gttgcttgtc
    10621 tatgttttcc atagccttat cttgaatag
//
LOCUS       DAOPID010000029        10433 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_7816_length_10433_cov_4.687608, whole genome shotgun sequence.
ACCESSION   DAOPID010000029 DAOPID010000000
VERSION     DAOPID010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 10433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10433
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7816_length_10433_cov_4.687608"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1941)
                     /locus_tag="PK641_02030"
     CDS             complement(<1..1941)
                     /locus_tag="PK641_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPG55167.1"
                     /translation="MSDLHVYKASAGSGKTTCLTESYLELMASGNASGNYNFEHRHIL
                     AVTFTNKATEEMKSRIIQLLAKVANGEKTLPYLDSKRAKEMLRSVLHDYSRFKISTID
                     RFFQQVIRAFVRELGINSNYAVELDHDQVIEDAVDEIILSLDQPNNKDVLDWLLSMAQ
                     EKIDAGKNWKVRDDLINFGKEIFKEDYLKYGSLITNINTIKNYKNKIYVIKKDRSSTI
                     INAAKDLKEKIGSADLLLTDFKNASRGPLCVLDKFSAGEIVGLSDTFLNLPNDPNNFY
                     TKNSPQKVIDASDWLCDDVANFIDIYNENIIEYNSAEAIYRFLPSLGVLHHIDEVVKT
                     MQKEANSLLISDSVKLLNTIVEDNDAPFVYEKVGTIIKHYLLDEFQDTSAMQWNNFKP
                     LIAESLGNGQKNLVVGDVKQSIYRFRNSDWKILGNQLESVDFKNQIESHTLSTNYRSD
                     AQIVSFNNDFFQYAVNALQDQYNDQLHEDLVGIISDDVLRKSYSDVLQNTKKDADSGY
                     VEIRLLEEKKVEDWRILAINNLFNSISELLEKKYNYGDITILVRTKKEAAQVAQSLLE
                     NNIPVVSDEALQISSSLEVCLIVSVIKRSINPNDDLNKLYIEHLCKEINVVQAEEELA
                     ADMPLYEFVEKIIDNYGLRSHKN"
     gene            complement(1938..2273)
                     /locus_tag="PK641_02035"
     CDS             complement(1938..2273)
                     /locus_tag="PK641_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HPG55168.1"
                     /translation="MDMDWKHKLGLVYSTNPDAMNSVDDSEEVKEQIPNQKQCLCLSF
                     QRRNGKPATIISGYEGSDDSLKELAKRLKVKCGVGGSVRDGEILIQGDLRVKLKELLH
                     SEGFKTKGI"
     gene            2430..3044
                     /locus_tag="PK641_02040"
     CDS             2430..3044
                     /locus_tag="PK641_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPG55169.1"
                     /translation="MKIIAIGWNYLDHNKELEHIGPKEPAVFCKPDSALLKGGKPFYI
                     PDFSDRIEYETELVVRIDKVGRNIAKKFANKYYHEIGLGIDFTARDLQAKFRETGSPW
                     ELCKAFDFSAPISEFFPISDFGGNVQDLHFSLDINGKTVQKGYTGDMIFDVDFIIEYV
                     SKFFTLKTGDLIFTGTPVGVGPVHQDDHLQGYIEDKPILDFYIK"
     gene            3046..6846
                     /gene="porU"
                     /locus_tag="PK641_02045"
     CDS             3046..6846
                     /gene="porU"
                     /locus_tag="PK641_02045"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HPG55170.1"
                     /translation="MDFKNKYLILLITFFASYFSMNAEQIIPVNILWESPRIFSTPSE
                     QETRLSFEDAQYIDELPFYTISVPIEEYSKTSDYKTSINDCKYEPLNTVESNLLKNAN
                     IADSIIFRTSISTERHNSSLDLRFCPIILKDGKYQKLISAKISINTLFKTKKRNVRIA
                     ADAYPSNSVLASGYWVKLAVRESGIYKLTYEEIVNMGVSSPANIRIFGYGGEIQEEDF
                     SKISIADKDDLKEIAIFMEKGSDEVFGSGDYILFWAQGVIKRTYNDLTGGYEFTPNYY
                     TDYGYYFATSDAGIGRRIEQAEKIEDTPTLTVNKSLAGVYHNVESINLLESGREWYGE
                     KFESGTLERNFQLPIENVATDEDALLNIEAIATSSSATTYTVSFNGNVLSNNIKISAV
                     GSSHQVANSGSISRNVSCSNFDYQTVTLKYNPAASSNVGYLNYITLSAYKHLNFNGSS
                     MEIFYPNDIEFDAVAGYEISGATSSTKIWNITDQSNITEIPTEYNGTLSFTALHNDLQ
                     TFLALNTQASFPAPTIVGAVANQNLHGLAQCDMIIVTPSEYMAAAERLAEHHRTHDGM
                     VVHIATPEQIYNEFSSGTPDATAYRRFLKVFYDRAALDGSKLPSSLLFFGVASHDNRG
                     YKHAKLPLISYQSEESLLSTRSYVTDDYYGLLDDNEGGSLASDKMDISVGRLPISSNT
                     EAENGVNKIIKYAQNSLTGTWKNIFAFMADDEDSNIHMSQADKLSKSLYSLNKAYQAH
                     KVYFDAYPLVQSTSGGTYPVARETILKDIKNGVLVFTYVGHGGPYTLSQEQTINQNDI
                     IAMYNDKQALWITATCDFSRYDNNTVSAGMNVFLNPNGGGIALLTTTRVVFSSDNFKL
                     GSAVYDYIVPKEGEDPLTLGEIVRRAKIDIGYDKNKLNFSLLGDPALVLNYPTKRIIT
                     DSINSLIPEDALMQALGLVTIKGHIADENGDSLKDFNGTVNLTIYDKEETMTTLANKG
                     NTPFNYQDYPNILFSGKASATNGCFETTFMVPKDINYKFGNGRILYYGVSDDKKTEAH
                     DYNESFQVGGSDPSAEPSEEGPNLKLYLNSPSFISGQVVNPTPVLFAHLNDSYGINVV
                     GAGIGHDITIKLNNDPNKTYVLNNYYESTLNDYKSGIVKYQFEPLEPGLYHLAFKVWN
                     LQNISSSAEIDFVVSEKSIPEISDFSISPNPASDYVDLTLMYDRPEIPVDVEFILYDM
                     QGRQYWNSKATVSTEGTYTSHIDLSGKIGDQIRPGIYLIKAIITTTEGTYDTKTQKLL
                     VNPQ"
     gene            6930..8144
                     /gene="porV"
                     /locus_tag="PK641_02050"
     CDS             6930..8144
                     /gene="porV"
                     /locus_tag="PK641_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HPG55171.1"
                     /translation="MKKKLSILLAISLSMPLIMFAQTPEDSEEPYQTFSPITTGVSFL
                     TVAPDARGGGLGDVGGATTPDGDSQYWNPSKYAFINGVGGANISYVPWLRKIVSDIDL
                     ISASGYYKIKDVQTVAMSFRFFSLGQVLIRREPDDAGYNVKPYDLALDASYSRILAKN
                     FSMGVALRFIYSDLTGGLITDDSKYLEPGWSIAADVSGYYGQVVDFAWGKGFGAFGFN
                     ISNIGKKVEYGDYRSAAFIPANLRLGGSFTMPFDRYNHMSLNVDFNKLLVPSRPVQRT
                     GESPEDYNIRLDEYYKMDPITGIFRSFADSPDGFVGELREINFSLGLEYDYNQQFFGR
                     IGYYNESQQSGNRKYVTFGAGFKLNVFSLDVAYVLSVAKNNPLDQTLRFTLGFNMGGL
                     KELASNTRDDSN"
     gene            8172..8648
                     /gene="ispF"
                     /locus_tag="PK641_02055"
     CDS             8172..8648
                     /gene="ispF"
                     /locus_tag="PK641_02055"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HPG55172.1"
                     /translation="MKIRVGFGYDVHRFAPERELWLGGIRIEHSLGLLGHSDADVLIH
                     AICDALLGAANLRDIGYHFPDTDPKYKGIDSKVLLKETGELISSHGFSISNIDATICA
                     EKPKLNPHIESMQKCLAQCLNILPEDISIKATTTEKLGFVGKEEGIAAYATVLIIK"
     gene            8748..>10433
                     /gene="pheT"
                     /locus_tag="PK641_02060"
     CDS             8748..>10433
                     /gene="pheT"
                     /locus_tag="PK641_02060"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPG55173.1"
                     /translation="MNISYNWLKKYVLLTIDPEETAKKLTSLGLEVETVETVQTIKGG
                     LEGLVVAQVVECIDHPDSDHLHITKVDAGDGNLLQVVCGAPNCRAGLKTIFAQVGTKL
                     YEEDKEFVIKKSKIRGVESFGMLCAEDEIGIGKSHDGIIELPKDAELGTPAKKYYNIE
                     DDTLIEVDITPNRADAISHFGVARDLAAGLGIKLIKPSVSAFSVDNHDFPIQVTVENH
                     EACPRYCGLSMTGINVKESPDWLKHRLISIGQRPINNVVDVTNFILQELGQPLHAFDG
                     DKIKGNHIFVKTLADKTPFKTLDEVTREISDNDLMICNAEEGMCIAGVFGGADSGVTE
                     NTKRIFIESAYFNPVWVRKTARRHSLNTDSSFRFERGCDPNITIYALKRAALLIKEVA
                     GGQVSSDIIDIYPNPIKDFKVELTYNKLNSLIGKEIPKDTVKSILSGLEIKIDNETED
                     CLSLSVPPYRVDVQRDVDVIEDILRVYGYNNILPGSDLKSSISYSITPDSNALQNRVA
                     KQLTGAGFIEILNNSLTKLSYYNSLNSYPAENAVKIMNPLGSDLSVMRQTLLLG"
BASE COUNT         3505 a         1833 c         1805 g         3290 t
ORIGIN      
        1 atttttgtgc gaacgcagac cataattatc aattattttt tctacaaact catacaatgg
       61 catatccgcg gctaactctt cttcagcttg tacaacatta atctctttgc ataaatgctc
      121 tatatacagc ttattaagat catcattcgg gtttattgac cgcttaatta ctgaaacaat
      181 caaacatact tctaatgacg atgaaatttg taatgcttcg tctgatacaa caggaatatt
      241 attttcaagc aaactttgag ccacttgagc agcttccttt tttgttctta ccaatattgt
      301 tatatcacca tagttatatt ttttttcgag taattctgaa atcgaattaa ataaattatt
      361 tatggccaat atgcgccaat cttcaacttt tttttcttca agtaaacgaa tctcaacata
      421 accactatca gcatcttttt ttgtattttg aagcacatcc gaatacgact tgcgaagaac
      481 gtcatccgaa ataattccaa ccaaatcttc atgcaattga tcgttatatt gatcttgcaa
      541 agcgtttact gcatactgaa agaaatcatt attaaatgat actatttgag catcacttct
      601 ataatttgta gatagagtat gcgattcaat ctgattttta aaatctacag actctaattg
      661 attcccaagg attttccaat cagaatttct aaaacgataa atactttgtt ttacatctcc
      721 tactacaaga tttttttgac cgtttcctaa actttctgct attaaaggtt taaaattatt
      781 ccattgcatt gcagatgtgt cctgaaactc atcaagcaaa taatgcttga taatggtacc
      841 tactttttcg tatacaaatg gagcatcgtt atcttctaca atagtattca acaattttac
      901 cgaatcgcta ataagcagag agttagcttc tttttgcatg gttttaacga cctcatcaat
      961 atgatgtaaa acacctaatg atggcagaaa cctatatata gcctctgctg aattatattc
     1021 tatgatattc tcattgtata tatctataaa atttgcaaca tcatcacata gccaatcgct
     1081 agcatcaata actttctgag gcgaattttt tgtgtaaaaa ttgttaggat catttggtag
     1141 atttaagaaa gtatcagaca aaccaactat ttcaccggct gagaatttat caagaacaca
     1201 taaaggacct cttgatgcat ttttaaaatc tgtaagaaga agatctgctg atcctatttt
     1261 ttcttttaaa tcttttgccg catttattat tgtagatgac ctgtctttct tgattacata
     1321 tattttattt ttgtaatttt ttattgtatt aatattagta atcaggctac catatttcaa
     1381 ataatcttct ttaaagattt cctttccaaa attaattaaa tcatctctta ccttccaatt
     1441 cttacctgcg tcaatttttt cttgcgccat tgacaatagc caatcaagca catccttatt
     1501 attcggttgg tcaagcgaca aaataatctc atctacggca tcttcaataa cttgatcgtg
     1561 atctaattca acagcataat tactattgat gcctaattct ctaacaaaag cgcgaataac
     1621 ttgctgaaaa aaacgatcta tagtgctgat tttaaatcga gaataatcgt gcaaaacaga
     1681 ccttagcatc tctttagcgc gttttgaatc caaataaggt aatgtctttt cgccattagc
     1741 tacttttgcc agaagttgaa taattctaga cttcatttct tctgtggcct tattggtaaa
     1801 agtaactgcc aatatatgtc tgtgttcgaa attataattt cccgaggcat ttcccgaggc
     1861 catcaattct aagtaagatt ctgtcaaaca agttgttttg cccgaacctg cagatgcttt
     1921 atatacgtga agatcactca tatgcccttt gttttaaatc cttctgaatg taataattct
     1981 tttagtttta cacgcaaatc gccctgtatg aggatttctc catcacgtac agacccacct
     2041 actccacatt taactttaag acgctttgca agttctttga gagagtcatc actaccttca
     2101 taaccgctta ttattgtggc aggtttacca tttcgacgtt gaaatgataa acacaaacat
     2161 tgtttttgat tgggaatttg ttctttcact tcttcagaat catctacact attcatcgca
     2221 tcaggatttg tagaatatac caaccctaat ttatgtttcc aatccatatc cattttataa
     2281 tgattattta ggctgaatta cttttgtttt agtacagaaa tgaatttatt caaaatttct
     2341 gaatgacaaa tttaactata attccgtttc atcactccta aaatacacat taaattcgta
     2401 tctttgccca aatatattgt ggcaaaagca tgaaaatcat tgcaataggt tggaattatt
     2461 tagaccataa caaagagctt gaacatatag gacccaaaga accggcagtt ttttgcaaac
     2521 ccgattctgc actgctaaaa ggaggtaaac cattttatat acccgatttt tcagatcgca
     2581 ttgagtacga aacagaattg gttgtacgta ttgataaagt tggtcgcaac attgcaaaaa
     2641 aatttgccaa caaatattat catgaaatag gattaggaat agattttacg gcgcgagatt
     2701 tacaagctaa gttcagagaa accggaagcc cttgggaatt atgtaaagcc ttcgattttt
     2761 cagcccctat aagcgagttc ttccctattt cagactttgg agggaatgta caagatttac
     2821 attttagttt ggatattaac gggaaaaccg tacaaaaagg atacaccggt gacatgattt
     2881 ttgatgtcga ttttatcatc gaatatgtaa gtaagttttt tacacttaaa accggcgatt
     2941 taatatttac aggaactccc gtaggagttg gaccggttca tcaagatgat catttgcaag
     3001 gatatataga agataaacca atcttagatt tctatattaa ataaaatgga ttttaaaaat
     3061 aaatacctga tattacttat tacgtttttt gcatcatact ttagtatgaa tgccgaacag
     3121 attattccgg taaatatatt atgggaatct ccgcgcattt tttcaacgcc aagtgaacaa
     3181 gaaacaagat taagttttga ggatgctcag tatatagacg aactgccatt ttataccata
     3241 tcagttccga ttgaagaata cagcaaaaca tcagactata agacaagtat taacgattgt
     3301 aaatatgagc ctttaaatac tgttgagtct aatttactca aaaacgccaa tatcgcagac
     3361 agtattattt ttaggacatc tataagtact gaaaggcaca actcaagcct tgacctgcgt
     3421 ttttgtccga taatattgaa agatggtaaa tatcaaaaat tgatttctgc taagattagc
     3481 ataaatacct tatttaaaac aaagaaaaga aatgtaagaa tcgcagctga tgcatatccg
     3541 tcaaattctg ttttagcaag cgggtattgg gttaaattag ctgttagaga atcgggcata
     3601 tacaaactta catacgaaga aattgtaaat atgggtgttt caagccccgc aaacattaga
     3661 atttttgggt atggaggtga aattcaagaa gaagatttct ctaaaatatc aatcgcagat
     3721 aaagacgact taaaagaaat cgcaattttt atggagaaag gatctgatga ggttttcgga
     3781 tccggagact acatactatt ttgggctcaa ggtgttataa aaagaactta taacgatcta
     3841 accggaggat atgaattcac tcccaattat tatactgact atggatacta ttttgctact
     3901 tcggatgcag gaattggcag aagaatagag caagctgaaa aaattgaaga tactcctact
     3961 cttactgtga ataaatcttt ggccggcgta tatcacaatg tcgaatcaat aaatttgctt
     4021 gaatcaggta gagaatggta tggcgaaaag tttgaatcag gtacacttga acgtaatttt
     4081 cagcttccaa ttgaaaatgt tgcaactgat gaagatgctc tattgaatat tgaagcaatt
     4141 gcaacatcat cttcagcaac cacctacact gtcagtttta atggtaacgt attaagcaat
     4201 aacattaaaa tatctgctgt cgggagttcg catcaagtag ctaattcagg ttcaatatca
     4261 agaaatgtaa gttgttcaaa ttttgattat caaacagtta cgcttaaata caatcctgct
     4321 gcatcatcaa atgtaggata cctcaattac atcacattaa gcgcatacaa acatctgaat
     4381 ttcaatggaa gttcaatgga aattttttat cctaatgata ttgagtttga tgcagttgcc
     4441 ggttacgaaa taagtggagc tacatcaagt acaaaaattt ggaatatcac agatcaatcg
     4501 aatatcactg aaattcctac cgaatacaat ggtacgcttt catttactgc attacataat
     4561 gatctgcaga cttttttagc ccttaacaca caggcatctt ttcctgctcc tacaattgtt
     4621 ggagcagttg caaatcaaaa tttgcatggt ttagcgcaat gcgacatgat aattgttact
     4681 ccttctgagt atatggcagc tgccgaaaga ttagcagaac accatcgcac acatgacggc
     4741 atggtagtac atatagctac tcccgaacaa atttacaatg aattttcttc aggaacgcct
     4801 gatgcaactg catatcgtcg ctttttgaaa gtgttttatg acagagccgc attagatgga
     4861 tcaaaattac caagttcatt actgttcttt ggagtcgcat cacacgacaa tagaggatac
     4921 aaacacgcaa agttgccttt aatcagttat caatcagaag aatcactatt atcaacgcgt
     4981 tcttacgtta cagatgatta ttacggcttg ttggatgaca atgaaggagg atcacttgct
     5041 tcagacaaaa tggacatttc ggtaggtaga ttgcctatat cttctaatac ggaagctgaa
     5101 aatggagtaa ataaaataat taaatatgct caaaatagtc tcaccggcac atggaaaaac
     5161 atctttgctt ttatggctga cgacgaagat agtaacattc acatgtctca agcagataaa
     5221 ttatctaaat cattatattc attaaataag gcataccaag cacacaaagt atactttgat
     5281 gcatatccgc ttgttcaaag cacaagcgga ggaacatatc ctgttgcacg cgaaaccatt
     5341 ttgaaagata taaaaaatgg cgtattggta tttacatacg ttggacatgg aggtccttat
     5401 acactttcgc aagagcaaac cattaatcag aatgacatta ttgccatgta taatgataaa
     5461 caagctctat ggattactgc aacatgtgac tttagccgtt atgataacaa tactgtatcg
     5521 gcaggaatga atgttttcct taatccaaat ggaggtggta ttgccttact tacgacaaca
     5581 cgtgtagtat tttcaagtga caatttcaag ctaggttctg ctgtatatga ttatatcgtt
     5641 ccaaaagaag gtgaagaccc tctaacactt ggagaaattg taagaagagc taaaattgat
     5701 ataggttacg acaaaaacaa actaaatttc tcgcttttgg gagatcctgc actagttttg
     5761 aattatccga caaaacgaat aataacagat agcattaata gcctgattcc tgaagatgca
     5821 ttgatgcaag ctcttggact tgttactata aaaggacata tagctgatga aaatggagac
     5881 tctttaaaag actttaatgg aactgtaaat cttaccattt acgacaaaga agagaccatg
     5941 actacattgg ccaataaagg aaatactcct tttaattatc aagattatcc taacattttg
     6001 tttagtggaa aagcaagtgc cactaatgga tgttttgaaa caacatttat ggttccaaaa
     6061 gatataaatt acaaatttgg gaacgggcgc atactatact acggcgtatc tgacgataaa
     6121 aagactgaag cacatgacta taacgaatct ttccaagttg gtggctcaga tccttctgca
     6181 gagcctagcg aggaaggacc taatttgaaa ttgtacttaa attctccatc atttatatca
     6241 ggacaagtag ttaaccctac ccccgtattg tttgcacatt taaatgattc atacgggatt
     6301 aatgttgttg gagcaggtat tggacatgac attacaataa agttaaacaa tgatccaaat
     6361 aaaacctatg tattaaacaa ttactacgaa tcgacgctaa atgactataa aagtggaata
     6421 gttaaatacc aatttgagcc attagagccg ggtttatacc atttagcatt taaagtttgg
     6481 aatttacaaa acatttcaag ttctgcagaa attgattttg tggttagcga aaaatctata
     6541 cctgaaattt ctgattttag tataagtcca aatcctgcat ctgattatgt agatttaacc
     6601 ctaatgtatg atagaccgga aatccctgtt gatgtagaat ttatattata tgacatgcaa
     6661 ggacgacaat attggaattc aaaggctact gtttctaccg aaggcacata tacttcacac
     6721 attgatttat ccggtaaaat tggagatcaa attcgcccgg gtatatattt aattaaagca
     6781 ataattacca caacagaagg gacttacgat acaaaaacac aaaagttgct agtaaatcca
     6841 caataaaatc caaaagatta cgttatcttt gtagccgttt ttttaaacgc tgttagataa
     6901 ttatgcacac acaatctatt tgtaaaataa tgaagaaaaa attaagtatt ttattggcta
     6961 tatcactaag tatgccatta atcatgtttg cacaaacgcc ggaagattca gaagagccgt
     7021 atcaaacatt ttcacctatt acaacgggag tatctttttt aacagtggca cctgatgcac
     7081 gcggaggcgg tttgggagat gtgggtggag caacaactcc tgatggcgat tctcaatatt
     7141 ggaatccatc aaaatatgct tttataaatg gcgtaggtgg agcaaatatt tcttatgtac
     7201 cttggttaag aaaaatcgta tctgatatcg atttaatttc ggcatcaggt tattataaaa
     7261 taaaagatgt tcaaactgta gctatgtcct tccgtttctt ttcgttagga caagttttaa
     7321 ttagaagaga accagatgat gcaggatata acgttaagcc atacgatttg gcattagacg
     7381 catcttactc gcgtatactt gctaaaaact tttcgatggg tgtagctctc cgttttattt
     7441 attccgattt aacaggtgga ctaataacag atgattctaa atatttagaa ccgggatggt
     7501 ctatcgcagc tgacgtgtca ggatattacg gacaagtagt tgattttgct tggggtaaag
     7561 gttttggtgc attcggtttc aatatttcaa atattggtaa aaaagttgaa tacggcgact
     7621 atagaagtgc tgcatttatt cccgcaaact tacgtttagg cggcagcttt acaatgccat
     7681 tcgacagata caaccacatg tcacttaatg ttgattttaa taagctattg gtaccttctc
     7741 gtcctgttca acgtacagga gaaagccctg aagattacaa tatacgttta gacgaatatt
     7801 ataaaatgga tcctattacc ggtatattcc gttcttttgc agattcacct gacggctttg
     7861 ttggagaatt acgtgaaatt aatttctcac ttggtttgga atacgattac aaccaacaat
     7921 tctttggacg tatcggttac tacaacgaat cacaacaatc aggaaatcgt aaatacgtaa
     7981 cattcggtgc aggtttcaaa ctaaacgtat tttcacttga tgtagcttac gtactttctg
     8041 ttgctaaaaa taatcctttg gatcaaacct tacgttttac acttggattt aacatgggtg
     8101 gtctaaaaga attagcaagc aataccagag acgatagtaa ttaatggcgt aaaaacccca
     8161 aaactattga catgaagatc agagtaggct ttggatatga tgttcaccgt tttgcacccg
     8221 agcgcgaact atggttgggt ggtatacgta tagaacattc cttaggacta ctaggacatt
     8281 ctgatgcaga cgttcttatt catgcaatat gtgatgcact acttggtgca gcaaacttac
     8341 gcgacatagg ataccacttt cctgataccg acccaaaata caaaggaata gatagtaaag
     8401 ttttattaaa agaaaccgga gaactaataa gttctcacgg tttctctatt tctaatattg
     8461 atgcaaccat ttgtgcggaa aaacctaaat taaacccaca cattgaatca atgcaaaaat
     8521 gcctcgctca atgtcttaac atactgcctg aagacatctc aataaaagca actaccacag
     8581 aaaaacttgg ttttgttgga aaagaagaag gtatagccgc ctacgctacc gtacttatca
     8641 taaaatagca tggttttttt ctaacatagc aaatataact gctgtgctat tttccttttt
     8701 caattaaaaa cactaatttt gcagcctaat attccaaaat agctgctatg aacatttcgt
     8761 ataactggct taaaaagtac gttttactaa caatcgaccc tgaagagact gctaagaaat
     8821 tgacatcttt gggattggaa gtagaaacag tcgaaacagt gcaaaccata aaaggaggct
     8881 tggaaggctt ggttgtagct caagttgtag aatgtatcga tcatcctgat tcagatcatt
     8941 tacatataac caaggttgat gccggtgatg gcaatttact acaagtagtt tgcggtgcac
     9001 caaactgccg cgcaggatta aaaaccatat tcgctcaggt tggtactaaa ctatacgaag
     9061 aagataaaga atttgtaatt aagaaatcaa aaatacgtgg tgttgaatct tttggcatgt
     9121 tgtgtgccga agatgaaatt ggcataggaa aaagtcacga tggtataatc gaattgccta
     9181 aagatgccga acttggtact cctgcaaaaa aatattacaa catagaagac gatacattaa
     9241 ttgaggttga tattactcca aaccgtgctg atgccatatc acactttggt gtggctcgcg
     9301 atttggctgc gggtttaggt ataaaactta ttaaaccttc tgtatcagca ttttctgttg
     9361 acaatcacga tttcccgatt caagtaactg tcgaaaatca cgaagcttgc ccaagatatt
     9421 gcggcttaag tatgaccgga atcaatgtaa aagaatctcc tgactggtta aagcataggt
     9481 tgatcagcat aggtcaacgc cctattaaca acgttgtaga tgtaaccaac tttattttgc
     9541 aagagttagg acaaccgcta cacgcatttg atggtgataa aattaaaggt aatcatatat
     9601 ttgttaaaac tttagctgac aaaactccat ttaagacttt agatgaagta actcgcgaaa
     9661 tttctgataa tgacctcatg atatgtaatg ccgaagaagg catgtgtata gcaggtgtat
     9721 ttggtggtgc agattcaggc gtaacagaaa acacaaaacg gatttttatt gaaagtgcat
     9781 attttaatcc tgtttgggtt agaaaaacag cacgtagaca tagcttaaat acagattcat
     9841 cattccgttt tgaacgtgga tgcgatccga atattacaat ttatgcactt aaacgtgctg
     9901 ctttgttgat aaaagaagtt gcaggtggtc aagtatcttc ggatattatt gatatatacc
     9961 ccaacccaat aaaagatttt aaggtagaac ttacatacaa taaattaaac agccttatag
    10021 gtaaagaaat accaaaagat actgtaaaat cgattctatc cggtttagaa ataaaaatag
    10081 ataatgaaac agaagattgt ttatcattat ctgtacctcc ttatagagtt gatgtacaaa
    10141 gagatgttga tgttattgaa gatatattgc gtgtatacgg atataataat attttacccg
    10201 gttccgattt aaaatcatca ataagttatt caatcacacc ggatagcaat gcacttcaaa
    10261 atagagtcgc aaaacaactt accggtgcgg gatttattga aattctgaat aattcattga
    10321 ccaaattatc gtattacaat tcattgaatt catatcctgc agaaaatgca gttaaaatta
    10381 tgaatccact tggcagcgat ttaagtgtaa tgcgccaaac attgttgttg ggt
//
LOCUS       DAOPID010000030        10260 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_8000_length_10260_cov_4.676923, whole genome shotgun sequence.
ACCESSION   DAOPID010000030 DAOPID010000000
VERSION     DAOPID010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 10260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10260
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8000_length_10260_cov_4.676923"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="PK641_02065"
     CDS             <1..1173
                     /locus_tag="PK641_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HPG55174.1"
                     /translation="QRLSLYDAPQKAMALGVYPYFRAISSDQDTVVRIDGKPVLMFGS
                     NSYLGLTNHPRIKEGSIKAIEKYGTGCAGSRFLNGTLDIHVELEERLAKLVGKDEAMV
                     YATGFTVNSGVIPCLCGREDYLLLDKLNHASIIEGSRLSLAKQFKYKHNDMESLEKFL
                     KNCDSDKIKLIVSDGVFSMEGDVCELPEIMKLAKKYNASVMIDEAHSLGFFGENGAGV
                     CEHFGMSKDVELIMGTFSKSLATIGGFIASNKDVINYLRHHSRTFIFSASITPAATGA
                     VLAALDVMKEETWRPKQLWANTNRMISSLRAAGFEMSNTTTPIIPLYVRDNEKTFRLT
                     SMLMQDGVFVNPVVSPAVPSEDTLIRISLMATHTFDQIDEAVEKITKNAKLLGIIK"
     gene            complement(1245..2921)
                     /locus_tag="PK641_02070"
     CDS             complement(1245..2921)
                     /locus_tag="PK641_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HPG55175.1"
                     /translation="MEDEVKKPLNFIEEIVESNIRDGINDGRIQTRFPPEPNGYLHIG
                     HAKAICMDFGIAQKYNGICNLRFDDTNPTKEDVEYVDSIQEDIKWLGFQWADVYYASD
                     YFQKLWDLAENLIKQGKAYIDEQSSEEIAKQKGTPTEAGKESPFRNRPIEENIALFHK
                     MNSGEIAEGSMVLRAKIDMASSNMHFRDPIMYRIITSHPHHRTGTKWKAYPMYDYAHG
                     QSDYFEGVTHSICTLEFVVHRPLYDWFIDQFANSAYRPRQYEFNRLNITYTVMSKRKM
                     LQLVQEGLVAGWDDPRMPTICGLRRRGYTPEAIKNFITKIGYTKYEGTIDISMLEAAA
                     REDLKPRATRVCAIFDPIKLIITNYSEGKSEPLTTENNPDDENAGTREITFSRELYIE
                     RGDFMEDAPKDFYRLTPNGREVRLKSAYIIQCTECIKNEAGEIIEIHCTYDPTSKSGT
                     EGGNRKVKSTINWVEIDSCISAEIRLYDRLFSIEDPSGSDEDFRNLLNKDSLRVIKNC
                     KVEPYLKRAKVLDHFQFLKQGYFTVDKDSKPESLIFNRTVSLKDNWAKKE"
     gene            complement(2935..3711)
                     /gene="lpxA"
                     /locus_tag="PK641_02075"
     CDS             complement(2935..3711)
                     /gene="lpxA"
                     /locus_tag="PK641_02075"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HPG55176.1"
                     /translation="MELNAFIHPNAQIGKNVIIDPFAFIDENVVIGDNTHIFSHATIF
                     SGARIGKNCRVFPGAVVGAIPQDLKFNGEETTAEIGDNTTIRECATVNRRTASKGKTV
                     VGNNCLIMAYSHVAHDCFLGNNIIIGNATQLAGEVVIDDWAILSGGCLIHQFCHIGQH
                     VIIQGGSRCGKDVPPYVTAGRDPLTFAGINLIGLRRRNFSPDQIQNVQECYRTIYQSG
                     LNNSQAVEKLEADLAPSIERDTVLNFLKTSQRGIIRGYND"
     gene            complement(3718..5100)
                     /locus_tag="PK641_02080"
     CDS             complement(3718..5100)
                     /locus_tag="PK641_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HPG55177.1"
                     /translation="MSKQRTIKQDFTLQGKGLHTGLEIIATFKPAEVDNGIRFVRIDL
                     EGKPEIPALAENVTASERGTVISKNNASVSTIEHAMATLFAHGIDNCTIEVNAPELPI
                     LDGSAAMYNQALIQAGIQEQDEDKNYFYVKEKIEYNYENGSSIVLLPDDKFSIHTLID
                     FNSPVLSNQYASLDSLENFDQEISVARTFVFVREIEPLLKQNLIKGGDLDNAIVIYDQ
                     LMSNEDINHLTDLMNLPRIQVDKLGYLNKKPLVFNNEPARHKLMDVMGDLALIGRPIC
                     GKVIATKPGHAVNTKVAKAIQHSFRKQEIQAPVYNPNAEPIMDINRVKQLLPHRWPFL
                     MVDKIIELKEKSIVGIKNVTGNETFFVGHFPEEPVMPGVLLVEAMAQVGGILVLDSVD
                     EPKKYSTYFLKIDNVKFRKKVSPGDTIIFRLSLLSELRRGCAYMQGYAFVGETMVAEA
                     QFMAQIIKNK"
     gene            complement(5130..6179)
                     /gene="lpxD"
                     /locus_tag="PK641_02085"
     CDS             complement(5130..6179)
                     /gene="lpxD"
                     /locus_tag="PK641_02085"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HPG55178.1"
                     /translation="MEFSAQQLASLLNGTIEGDASVKVSNFSKIEEGKPSTLTFLSNP
                     KYEQYIYTTKADIVLVNNDFKPKQAISATLIRVNDPYMALATLLKLSESHKQNPIGID
                     PLAFVSKTAKIGKNVSIGAFAYIGDNVTIGDDTCIYPNSYLGPDVSVGSNTIIYPNVS
                     IYKECVIGNNCILHSSCVIGADGFGFARDESGKYVKMPQNGNVILEDDIEVGASTTID
                     RGSMGSTIISRGVKLDNQIQIAHNVEIGENTVMAACTGISGSTKIGKDCVFAGKIGSV
                     GHISIADNVIIAGGSNLTKNITEKGTIHQGSPAIPIGKAKRCYAVYNNLPELQKTVYE
                     LQKEIKELKDLINNK"
     gene            complement(6230..7444)
                     /locus_tag="PK641_02090"
     CDS             complement(6230..7444)
                     /locus_tag="PK641_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005679952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPG55179.1"
                     /translation="MPNNKRKIINDPVFGFITIPSDLIYDLLQHSYLLRLTRIKQLGL
                     TSYVYPGTQHTRFQHSLGALYLMTEAVSTLRSKGVEITDDEADAVYAAILLHDVGHGP
                     FSHTLENCFFPNISHEELTIKICELLNEEMDGALNSTLDVLQDNHKKKFLREMVSGQL
                     DMDRLDFIRRDSFFSGVSEGNIGSARIIKMLNIKDNKLVVEAKGIYSIENFLMSRRLM
                     YWQVYFHKTAYAAEKMIINTIRRAKYIQKSGTKLFAPPALQYFLDNQNPELNRSSLTN
                     FLSLDDSDIWTSMKVWISQPDKVLSTLCDGIVNRKLFKMDIGNKKIDDSKIHMIKSEI
                     ANRLNISLEDAEYLTSSASVSQHIYKGDETNIGILYNNGNVKTIQEASDMFDINVLSR
                     KITKYYFCYYRY"
     gene            complement(7453..8283)
                     /gene="pyrF"
                     /locus_tag="PK641_02095"
     CDS             complement(7453..8283)
                     /gene="pyrF"
                     /locus_tag="PK641_02095"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HPG55180.1"
                     /translation="MTREQLFENIRNKKSFLCVGLDSDIAKIPEHLFENEDALFEFNK
                     AIIDATAPYCVAYKPNLAFYESLGLEGWDVLDRTVEYIKSNYPDQFIIADAKRGDIGN
                     TSTMYAKTVFGNMDFDSVTVVPYMGEDSVKPFLEYEGKWVTVLALTSNKGAFDFQFSE
                     DAEGVKLFEKVLKVSSKWGSADNMMYVVGATKAEMLSDIRNIIPEHFLLVPGVGAQGG
                     SLQEVAKNGLNNQCGLLVNSSRAIIYADNTENFATSAAIAAQAVQAEMESILKQNNLI
                     "
     gene            complement(8295..9404)
                     /gene="prfA"
                     /locus_tag="PK641_02100"
     CDS             complement(8295..9404)
                     /gene="prfA"
                     /locus_tag="PK641_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPG55181.1"
                     /translation="MGENQLITKLEGLYHKFEEVSTFITDPAVIADMKRFVKLNKEYH
                     ELEQIVNAGKRYKKLLANLSEAKELLDTENDEEMRSMAKAEIDEIEPQIATLEEEIKL
                     LLIPSDPEDNKNAIVEIRGGTGGDEASIFAGDLFKMYTKFCESKGWKVEVSTLSEGTA
                     GGYKEIIFNVSGDKVYGIMKYESGVHRVQRVPATETQGRVHTSAATVAVLPEADEFDV
                     EINEGEIKWDTFRSGGAGGQNVNKVESGVRLRYIWKNPISGVPEEILIECTETRDQPK
                     NKERALSRLRTMIYDREHQKYVDDIAARRKTLVSTGDRSAKIRTYNYPQGRITDHRIN
                     YTIYNLSAFMNGDIQDVIDKLMVVENTERLKDSEL"
     gene            complement(9411..>10260)
                     /locus_tag="PK641_02105"
     CDS             complement(9411..>10260)
                     /locus_tag="PK641_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007215345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HPG55182.1"
                     /translation="IGRNKILIPGEVISAIINGTDELLAELREMGVGVYATGGETADV
                     GDLVRTIIVDSTVTCRMKRSDVIDNANIKAGDVIVGMASFGKATYEKQYNGGMGSNGL
                     TSARHDVFAKYLAAKYPESYDAAVPSELVYSGKLKLTDEVKDSPIDAGKLVLSPTRTY
                     APVIKKMLDEMRPSIHGMVHCSGGAQTKVLHFVENVHVIKDNLFPIPPLFKTIQEQSG
                     TDWSEMYKVFNMGHRMEVYVSPADAERVITISKSFGIDAKIIGHIEADKKTRLTISSE
                     FGTFEY"
BASE COUNT         2971 a         1899 c         1737 g         3653 t
ORIGIN      
        1 cagcgtttat cattgtatga tgcccctcaa aaggctatgg cgttaggagt ttatccatat
       61 ttccgtgcaa tttcatctga tcaggataca gtagttagga ttgacggaaa accggtttta
      121 atgtttggct caaacagcta tttgggatta accaatcacc ctcgtattaa agaaggctca
      181 attaaagcta ttgaaaagta tggtacagga tgtgcgggtt ctcgtttctt aaatggaaca
      241 ttagatatcc atgtggaact agaggaacgt ttagctaagt tagttggcaa agacgaggcc
      301 atggtttatg caacaggttt tacggttaat tcaggtgtta taccttgctt atgtggtcgt
      361 gaagactatt tattgctgga taaacttaat catgcttcaa ttattgaagg atcacgttta
      421 agtttggcta agcaatttaa gtataaacac aatgatatgg aatctcttga aaaattcctt
      481 aagaattgtg attctgataa aattaaattg atagtttctg atggcgtatt cagtatggaa
      541 ggtgatgttt gtgaattacc tgaaataatg aaattggcaa aaaaatacaa tgcttcagta
      601 atgattgatg aggctcattc attaggcttt ttcggtgaaa atggagccgg tgtttgcgaa
      661 catttcggca tgtcgaaaga tgtagaacta ataatgggaa cattcagtaa atctttggca
      721 acaataggcg gatttattgc ttcaaataaa gatgttatta attatttaag gcatcattct
      781 cgtacattta tatttagtgc aagtattact cctgcagcta caggtgctgt gttagcagct
      841 ttagatgtta tgaaagaaga aacatggcgt ccaaaacaat tgtgggcaaa taccaacaga
      901 atgataagct cattgagagc cgccggattt gaaatgagta atacaactac accaatcatt
      961 ccattgtatg ttcgtgataa tgaaaaaaca ttccgtttaa caagtatgtt aatgcaagat
     1021 ggtgtttttg ttaacccggt tgtttctcct gcagtacctt ctgaagatac attgattaga
     1081 atttcattga tggctactca tacatttgat caaattgatg aggcagtaga gaaaatcacc
     1141 aagaatgcta aattattagg aatcataaaa taattttaga ttctttagat ataaaaaatg
     1201 gcatacttaa aaagtatgcc atttttattt tatgctatat ttaattattc ttttttagcc
     1261 caattatctt taagcgatac ggtgcgatta aatattaatg attctggttt tgaatctttg
     1321 tcaacagtaa agtatccttg ttttagaaat tggaagtgat ccaatacttt ggctcttttt
     1381 aaataaggct caactttaca attttttatt acacgtaaag agtctttatt taatagattc
     1441 ctaaaatctt catcagaacc cgatgggtct tcaatagaaa acaatctatc gtataaacga
     1501 atttctgctg atatgcaaga atctatctca acccaattga ttgtactctt aacttttctg
     1561 ttaccgcctt cggttccact tttacttgta gggtcgtatg tacaatggat ttcgataatt
     1621 tctcctgctt catttttgat acattcagta cattgtataa tgtatgcact ttttaatctt
     1681 acttcacgac cattgggagt taaacgataa aaatctttag gagcatcttc cataaaatca
     1741 cctctttcaa tatataattc acgagaaaaa gtgatttcac gtgtacccgc attttcgtca
     1801 tcaggattat tctctgttgt tagtggctca gatttccctt cagaataatt tgtaattata
     1861 agtttaatag gatcaaagat ggcgcaaaca cgagtggctc ttggctttaa gtcttcgcgt
     1921 gcagctgctt ctaacataga tatatcaatt gtaccttcgt attttgtata tcctatcttt
     1981 gtgataaagt ttttaatagc ttcgggagta tatccgcgtc gtcttaagcc acagatggtc
     2041 ggcatacgag gatcatccca acctgcaaca agtccctcct gtaccaattg tagcattttt
     2101 cgtttactca ttacagtata tgtaatattt aaacgattaa attcatattg acgaggtctg
     2161 tatgctgagt tggcaaattg atcaataaac caatcgtata atggtctgtg tacaacaaat
     2221 tctaatgtgc agatagagtg tgtaacgcct tcaaaataat ctgattgtcc atgtgcgtaa
     2281 tcgtacatcg gatatgcttt ccatttagta ccggtacgat ggtgcggatg acttgtaata
     2341 atgcggtaca taataggatc acgaaagtgc atatttgacg atgccatatc tattttggca
     2401 cgtaatacca tgctgccttc agcaatttct ccgctattca ttttatggaa taaagcaata
     2461 ttctcttcaa taggtctgtt tctaaaagga ctttctttgc cggcttctgt cggagttcct
     2521 ttttgctttg caatttcttc agaagattgt tcgtctatgt atgccttacc ttgttttatt
     2581 aagttctcag ccagatccca taatttttga aagtaatcag aagcgtagta tacgtctgcc
     2641 cattgaaatc ctaaccattt aatatcctct tgaatagaat caacatattc aacatcttct
     2701 tttgtagggt tagtatcatc aaatcgcaga ttgcaaattc cattgtattt ttgagctata
     2761 ccaaagtcca tgcaaattgc ttttgcatga ccaatatgaa ggtagccgtt tggttcaggc
     2821 ggaaatcttg tttgtattcg cccgtcatta ataccatctc ttatattgga ctctacaatt
     2881 tcttcaatga aattcagcgg tttttttact tcatcttcca ttattagtat ctgcttagtc
     2941 gttgtatccg cgtataattc cgcgttgtga tgtctttaag aaatttaata cagtgtctct
     3001 ttctatagat ggagctaagt ctgcttctaa cttttctaca gcttgcgaat tatttaaacc
     3061 tgattgataa attgtgcgat aacactcttg aacattttga atttgatcag gtgagaaatt
     3121 tctgcgtcta agaccaatca gattaatacc tgcgaaagtt agaggatcac gtcctgccgt
     3181 tacgtaagga ggaacgtctt taccacagcg tgagccacct tggataatga catgctgacc
     3241 tatatggcag aattggtgaa ttaagcatcc tccgcttaaa atagcccaat catcaatcac
     3301 tacttctcct gctaattgtg ttgcattacc tataataatg ttgttaccaa gaaaacaatc
     3361 atgagctacg tgcgaatatg ccattataag gcaattatta cctacaactg ttttaccctt
     3421 tgatgcagtt ctacggttta cggtagcaca ttcacgaata gttgtattat caccgatttc
     3481 tgctgtagtt tcttcaccat tgaattttaa atcttgtgga attgctccaa caactgcgcc
     3541 cggaaatact cgacaatttt tacctatgcg tgcacctgaa aatattgttg catgtgaaaa
     3601 aatatgggtg ttgtctccta taacaacatt ttcatcaata aatgcaaaag gatcaattat
     3661 aacatttttg cctatttggg cattaggatg aatgaaagcg tttagctcca tagctattta
     3721 tttgtttttt attatttgag ccataaattg ggcttcggct accatagttt ctccaacaaa
     3781 tgcatatcct tgcatatatg cacaacctct gcgaagttca cttaatagac ttagtctaaa
     3841 tataatagta tctcccggcg ataccttttt gcgaaactta acattatcta tttttaaaaa
     3901 gtaggtagaa tattttttgg gttcgtcaac agaatctaat actaaaatac ctccaacttg
     3961 tgccatagct tctactaaaa gaactccggg cataaccggt tcttcaggga aatgaccaac
     4021 gaaaaaggtt tcatttcctg ttacattttt tataccaaca atacttttct cctttagctc
     4081 tattatttta tcaaccatca aaaacggcca acgatgaggc agtaattgtt ttacacgatt
     4141 gatgtccata ataggttctg cattcggatt gtaaacagga gcttgtattt cttgtttgcg
     4201 gaaactgtgt tgaattgctt ttgcaacttt tgtgttaaca gcatgacccg gtttggtagc
     4261 gattactttt ccgcatatag gacgacctat taaagctaaa tcgcccatca catccattag
     4321 cttatgacgt gccggctcgt tattgaatac taatggtttt ttatttagat atccaagttt
     4381 gtcaacttgt atacgaggta aattcattaa atcggtcaaa tgattaatgt cttcatttga
     4441 cattaattga tcatatatta caatagcatt gtctaaatcg cctcctttaa taaggttttg
     4501 cttaagcaat ggttctattt cgcgtacaaa tacaaaagta cgagcaacgg atatttcttg
     4561 atcaaaattt tctaatgaat ctagtgaagc atattgattt gataataccg gagaattaaa
     4621 atcaattaat gtgtgtatgc taaatttatc gtcaggaagt aaaacaatag aagaaccatt
     4681 ttcgtaatta tattcaatct tctctttaac gtaaaaataa tttttatctt catcttgctc
     4741 ttgaattccg gcttgaataa gtgcttgatt atacatggca gcacttccat caagtatagg
     4801 aagttccggt gcatttactt caattgtaca attatcgatg ccatgtgcaa aaagtgttgc
     4861 cattgcatgt tctatcgtac ttactgatgc attgtttttt gaaataacag tacctctttc
     4921 tgatgctgta acattttcag ctaaagcagg gatttcaggt ttcccctcca gatctatgcg
     4981 cacaaagcgt ataccgttat caacttcagc cggtttgaaa gtggctatta tctcaagtcc
     5041 tgtatgaaga cccttgcctt gcaaggtgaa atcttgttta atagtacgtt gtttcgacat
     5101 tttttgttat tagtatgcag atgattagtt tacttattat taatcaaatc tttaagttct
     5161 ttgatctctt tctgtaattc gtaaacagtc ttttgtaatt caggtaaatt attataaaca
     5221 gcatagcatc ttttagcttt tcctattggg atagcaggag atccttggtg tatagttcct
     5281 ttttctgtta tattctttgt taaatttgag cctcccgcta taattacatt atctgcgata
     5341 cttatatgac caacagatcc gatttttccg gcgaagacac aatctttacc aatcttagtt
     5401 gaaccggaaa ttccggtgca tgctgccata actgtgttct ctcctatctc aacattatgg
     5461 gcaatttgta tttggttgtc taatttcact cctctagata ttattgttga ccccatgcta
     5521 cctctatcaa ttgtagtaga agctccaact tcaatatcat cttctaaaat tacattgcca
     5581 ttttgcggca tctttacgta tttgccactc tcgtcgcgtg caaagccaaa accatctgca
     5641 ccaataacgc atgatgagtg taaaatacag ttgttaccaa taacacattc tttataaatg
     5701 cttacgttcg gataaataat agtgttgctg ccaacagaaa catccggacc taagtaactg
     5761 tttggatata tacatgtatc gtcgccaatt gtaacattgt ctccgatata tgcaaaagct
     5821 ccaatagaaa cgttttttcc aatttttgct gtctttgaga caaacgcaag ggggtcaatt
     5881 cctataggat tttgtttgtg gctttctgaa agttttaaaa gtgtagctaa tgccatatat
     5941 gggtcattaa ctcgaataag tgttgcagat atagcttgtt ttggtttgaa atcattattg
     6001 actaaaacta tatcagcttt tgtggtgtaa atgtattgtt cgtattttgg atttgataag
     6061 aaggttagtg tactaggttt accttcttct atttttgaaa aattgctaac ttttacagat
     6121 gcatctcctt ctatagttcc gtttaaaaga cttgccaatt gttgcgctga aaactccatg
     6181 ttttttttat acataatttc ggcaaaggta ctattttttt ttcgataaat cagtagcggt
     6241 agtagcaaaa atagtatttt gtaattttcc tggataacac atttatatcg aacatatcag
     6301 aggcttcctg tatggttttt acattgccgt tattgtataa aataccaata ttcgtttcgt
     6361 cgcctttata tatatgttga gaaacagatg cggagctggt aagatattcg gcatcttcta
     6421 aacttatatt taagcgattt gcgatttctg atttaatcat atgaattttg ctatcgtcaa
     6481 tttttttgtt gccgatatcc attttgaata gttttctatt tactattcca tcacaaagag
     6541 ttgacagaac tttatcaggt tgagatatcc aaaccttcat tgatgtccaa atatcagagt
     6601 catcaagtga caagaaattt gttaaagaac tgcggtttaa ttccgggttt tgattgtcta
     6661 gaaagtattg taatgccggt ggtgcaaata atttagttcc tgatttctgt atgtatttag
     6721 cacgtctaat agtatttata atcatttttt ctgcagcata agctgttttg tgaaaatata
     6781 cttgccaata cattaatcta cggctcatta agaaattttc tattgaatat atgcccttag
     6841 cttcaacaac taatttgtta tctttaatgt tgagcatttt gataatgcgt gcggaaccta
     6901 tatttccctc gcttactccc gaaaaaaaac tgtctctgcg tataaaatcc aatcggtcca
     6961 tatctaattg acctgatacc atttcgcgaa gaaacttttt tttgtgattg tcttgaagaa
     7021 catctaatgt agagtttagt gctccgtcca tttcttcatt aagcaattcg catattttga
     7081 tagttagctc ttcgtgagaa atattaggaa aaaaacaatt ttctaatgtg tgagaaaatg
     7141 gtccgtgtcc aacatcgtgt aataaaatgg ctgcataaac tgcatcagct tcatcatcag
     7201 taatttctac tccttttgaa cgaagtgtcg aaactgcttc tgtcattaaa tataatgctc
     7261 cgagtgagtg ttgaaatctg gtgtgctgtg ttccaggata aacatacgaa gttaaaccga
     7321 gttgcttaat acgtgtaagt ctaagtaaat aagaatgttg tagtaagtca taaatcaaat
     7381 cgctcggaat cgtgataaaa ccgaagacag gatcatttat tatttttcgt ttgttattag
     7441 gcacttgatt gattaaatca aattattttg ttttaaaatt gattccatct cagcttgaac
     7501 agcttgtgct gctatagccg ctgaagtagc aaaattctcc gtattgtcgg cgtatataat
     7561 tgcgcgtgaa gaatttacca ataatccgca ttgattattt aaaccattct ttgcaacttc
     7621 ttgcaaacta cctccttgag cgcctacgcc aggtaccaat aaaaagtgtt ctggaattat
     7681 gttacgtata tcgcttagca tttctgcttt tgtagcacca acaacataca tcatattatc
     7741 tgcacttccc catttagaag atactttcaa aaccttttca aataatttta cgccttctgc
     7801 atcttctgaa aattgaaaat cgaaagcgcc tttatttgat gttaaagcta aaacagttac
     7861 ccatttaccc tcatattcta aaaatggctt aacgctgtct tctcccatgt aaggtacaac
     7921 tgtaacacta tcaaaatcca tattaccgaa aactgtcttt gcatacattg ttgatgtatt
     7981 gccaatatca ccgcgttttg cgtctgcaat aataaattga tcaggataat tgcttttgat
     8041 atattcaacc gtgcgatcta atacatccca tccttcaaga cctaggcttt cgtaaaatgc
     8101 aagattaggt ttataagcta cacaataggg tgctgttgca tcgataattg ctttgttaaa
     8161 ttcaaaaaga gcatcttcat tttcgaatag gtgttccgga atttttgcaa tatcagaatc
     8221 taatcctaca cataaaaatg attttttgtt tcgtatattt tcaaaaagtt gttcccttgt
     8281 catatattgt tattttataa ttcgctgtct tttaagcgtt ctgtattttc tacaaccatt
     8341 aatttatcta ttacatcttg aatgtctcca ttcataaatg cggataaatt gtaaatggta
     8401 taatttatgc ggtggtctgt aatacgtcct tgcggataat tgtatgttct gatttttgca
     8461 gatctatcgc ctgtagatac aagtgttttt cgtcttgcag ctatatcgtc aacatatttc
     8521 tgatgctcac gatcgtatat cattgttctc aatcgagata atgcacgttc tttgttttta
     8581 ggttggtcgc gtgtttcagt acattcaata aggatttctt ccggaactcc gcttataggg
     8641 tttttccaaa tatagcgaag tctgacacct gattcaactt tatttacatt ttgacctcct
     8701 gcacctccac tacgaaatgt atcccatttg atttcgcctt cgtttatttc aacgtcaaac
     8761 tcatcagctt caggcaatac tgcaacagtt gctgcagatg tatgtactct accttgagtt
     8821 tcggtagcag gaaccctttg cacacgatgt actcctgatt catatttcat gattccgtaa
     8881 accttatctc ccgaaacatt gaagattatt tctttgtatc ctccggcagt accttcactc
     8941 aatgtactaa cctcaacctt ccaaccttta gattcgcaaa atttggtgta cattttaaat
     9001 aaatctccgg caaatataga tgcttcgtct cctcctgtac cgcctctaat ttctactatt
     9061 gcattcttgt tatcttccgg gtcagatggg attagtaaaa gtttgatttc ttcttccaaa
     9121 gtggctattt gtggctcaat ttcatcaatc tcagctttag ccatagaacg catttcttcg
     9181 tcattttcag tatctaaaag ttctttagcc tctgataggt tcgccaataa ttttttgtat
     9241 cgtttacctg catttacaat ttgctctagt tcgtgatatt ctttgtttaa ttttacaaag
     9301 cgtttcatat ctgctataac tgcaggatcg gtaataaaag tcgatacctc ttcgaactta
     9361 tgataaagtc cttctaattt cgttataagt tgattctctc ccatatagaa tcaatattca
     9421 aaggttccga attcgctact tattgtcaaa cgagtctttt tgtcagcctc gatgtgacca
     9481 ataattttag catcaatacc aaatgatttt gaaatggtaa taaccctttc tgcatcagcc
     9541 ggagatacat aaacttccat gcgatgcccc atgttaaata ccttgtacat ttcagaccaa
     9601 tcagttccac tttgttcctg aattgtcttg aaaagaggag gaatagggaa tagattgtct
     9661 ttaataacat gaacattctc aacaaaatgt agaacttttg tttgtgctcc accagagcaa
     9721 tgcaccatac catgtattga tggtcgcatt tcgtccagca tttttttgat aacgggagca
     9781 tatgtacggg taggagataa aaccaattta ccggcatcaa taggtgagtc tttgacttcg
     9841 tcagtaagct ttaattttcc tgaatatact aattcggatg gtactgctgc atcataactt
     9901 tcaggatatt ttgctgcaag gtattttgca aatacgtcgt gtcttgcaga tgttagtccg
     9961 ttactgccca tacctccatt atattgtttt tcgtatgttg ctttaccaaa cgatgccata
    10021 ccaacaatta catctcctgc ttttatattt gcgttgtcaa tcacatcgct tcgtttcatt
    10081 cggcaagtta cagtgctgtc aacaattatt gttctaacaa gatcaccaac atctgccgtt
    10141 tctccgcctg tagcatatac gccaacgccc atttcgcgta gttctgcaag tagttcgtct
    10201 gtgccgttta ttattgccga aataacttct ccgggaatta atattttatt tcttccaatg
//
LOCUS       DAOPID010000031         9525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_8915_length_9525_cov_4.554699, whole genome shotgun sequence.
ACCESSION   DAOPID010000031 DAOPID010000000
VERSION     DAOPID010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9525
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8915_length_9525_cov_4.554699"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="PK641_02110"
     CDS             complement(<1..1026)
                     /locus_tag="PK641_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HPG55183.1"
                     /translation="MNRPDRIKLYFKDTSFASLMTQRIYNILLVASKYDAFTLEEDGR
                     IDEQIFNEYTSLNLRYPPRFTLASTMKDAKDLLKARSFELIIVMPSGDNSEVFELSKE
                     FKSQRPSIPIVLLTPFSREMFKKVFGHEDFTGIDYVFSWLGETDLLLAIVKLIEDKMN
                     VDKDIASVGVQAILFIEDSIHFYSAILPYLYKHVFVQSRSFMTEALNDHERMLRMRGR
                     PKILLARSYEEGLKLYDKYSKNILGVVSDVRFPRNGVMDGAAGINLCSYIRSKDKFLP
                     IIIESSEDKNKPLAEKLGAYFINKNNETMHLELKDAVTTNFGFGDFNFINPENKEVVA
                     VARNLRDL"
     gene            complement(1048..1431)
                     /locus_tag="PK641_02115"
     CDS             complement(1048..1431)
                     /locus_tag="PK641_02115"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPG55184.1"
                     /translation="MIVENHIQKDEFAKSIGIEILEAYNGYAKMRMPITKKHLNGLGS
                     VQGGALFTLADYTMGAASNSMGGECVTLNAQIDYFRGKSEGVLISIAKPVKQGKTIIL
                     YDVEVRMEDTDQLIAVFRGTAFRKR"
     gene            complement(1501..2967)
                     /locus_tag="PK641_02120"
     CDS             complement(1501..2967)
                     /locus_tag="PK641_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPG55185.1"
                     /translation="MKKILTLITVLAFSFTLASAAKNTASAFSDSTGGLNDFKDFTHW
                     SLSINGGLSQFDGDVTQKYNDIFSQSTVSWTVGLDIEYTWNPYWGLIAQFQYMPFLGA
                     TKTTEYGSNTFTGNMYEPTLMASLNLLNLFGQYRSTWKWGFYLNAGLGLTFYDMQTTP
                     IPGAPIGTVEQAAITRATNEGKTPAEIAAAAAAAGPQDITDGMAICFPVGLNVEYNFN
                     KYLALGLNAHYRFHNKDYFEGEAYTKGTMNDGEFTATLNFRVKFAPNKKEGGHMRNIS
                     PYAYRELRTGDKNFQDQLDSLKKRVAAVEDTLGNNVLPRIAALEAQQATRPDGDGDGV
                     PDFRDREPNTPQGSFVNYWGESIPQEIMQSAKCCDEVKSVLAAMGAGIDYEMSVYFPF
                     DKYYLTNVAKSNVAKAAQKLKEDPELQVELRGYCDFPGSGDYNMKLSSNRVETVKKEL
                     IEKYGIDASRISVTPKGKMQNPPMKEYKNRRCDFYFHK"
     gene            complement(3054..4430)
                     /gene="miaB"
                     /locus_tag="PK641_02125"
     CDS             complement(3054..4430)
                     /gene="miaB"
                     /locus_tag="PK641_02125"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HPG55186.1"
                     /translation="MENQNGKPTDYASAIFACEKKLFLETYGCQMNIADSEVVAAVMQ
                     MAGYVITEDISEADAIFINTCSIRDHAEQKALSRLEFFQSLKRKNNKLIVAVLGCMAE
                     RIKEDLLSRKVIDMVVGPDAYLDLPNLIASAEQGSRVVNVESSVVQTYKDVIPSRIGH
                     NTISGFVSIMRGCNNFCSYCIVPYTRGRERSREYQSILNEVLDLKEKGYKELTLLGQN
                     VNSYKFEGMGFAELLDKLAGEVPDMRIRFTTSHPKDMDEEMVKTIAKHHNLCKHIHLP
                     IQSGSNRILQLMNRKYTREWYLDKIAMIRKHIPDCGITTDLFTGFHSETEEDHAQTLA
                     LMREVAFDSAFMFKYSEREGTYASKHLPDDVPEEIKVKRLQEAIDLQLQLSLQSNEND
                     IDKQFEVLVEGYSKRSREQLFGRTSQNKVVLFDKGNVKRGDYVIVEIEKASPATLFGS
                     ILTNELKK"
     gene            4982..5455
                     /gene="greA"
                     /locus_tag="PK641_02130"
     CDS             4982..5455
                     /gene="greA"
                     /locus_tag="PK641_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006254759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HPG55187.1"
                     /translation="MATPNYMTEEGYKKLMEEINRLESVERPKISRQIAEARDKGDLS
                     ENAEYDAVREAQGLLELKIAQMKATVSNVRIIDETQLGDGTIQILSKVTIKNVKTGQK
                     MTYTLVSEAEANIREFKMAISTPIAKALIGKKKGDHVTVEAPSGIIEFEILEAGF"
     gene            5467..5865
                     /locus_tag="PK641_02135"
     CDS             5467..5865
                     /locus_tag="PK641_02135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HPG55188.1"
                     /translation="MASIFSKIVAGEIPCYKVAETDKYFAFLDINPLVKAHTLVIPKQ
                     EVDYIFDLSDEDLAGLHIFAKIIADAIKKAYPCKKVGEAVIGLEVPHAHIHLIPIQKE
                     SDMLFSNPKLKLTAEEFKEIAANIQNAIKA"
     gene            complement(5950..7185)
                     /locus_tag="PK641_02140"
     CDS             complement(5950..7185)
                     /locus_tag="PK641_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HPG55189.1"
                     /translation="MSESKLFTPFQLGPITLRNRTIRSAAFENMCYDNKPSQDLFNYH
                     TSVAKGGIGMTTIAYCAVNRNGVSFDGQLWMREEIIPELKKLTDAIHAEGAKASIQLG
                     HCGNMTHYYTCGGMPVGASSGFNLYSPTIMRGLKISEINDLVKDFGKSVDMCRKAGFD
                     CVEIHAGHGYLISQFLSPYTNHRKDEYGGSLDNRMRFMRQVLHEVMQHAKDDMAVVVK
                     TNMYDGFKGGLELPDCLTVAKEIEKCGVHGIVLSAGFVSKAPMHVMCGAMPLKTLGHY
                     MNPWKFWWLKAGLKVVGRQMIPTVPFKEAYFLEPALKFRAEIKCPLIYVGGLVSRAKI
                     DEVLDKGFELVQMARALVHDPEFVNKMKESDSNYRNGCKHSNYCIGRMYTLEMKCHHC
                     VDNLPKSLVKEIEKAEAKL"
     gene            complement(7207..7974)
                     /locus_tag="PK641_02145"
     CDS             complement(7207..7974)
                     /locus_tag="PK641_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HPG55190.1"
                     /translation="MDLAQFNFFLLIMTIIALVVFVSLFFVEAGYGKLISEKWGPSIN
                     NKVAWVMMECPVFFVIAYLWWMSDRTFEIVPLIMFLLFELHYFQRSFIFPFLLKGDSK
                     MPIAIMLMGVLFNIANGYIQGRWIFYLAPENMYTESWLTSWQFILGTSIFIIGLIINW
                     NSDHVIRNLRAPGDTNHYLPQKGMYKYVTSANYFGEILEWGGFAILTWSTAGAVFLWW
                     TCANLVPRANSIYKKYKVEFEQEFKGKHLKRVFPFIY"
     gene            complement(8024..8983)
                     /locus_tag="PK641_02150"
     CDS             complement(8024..8983)
                     /locus_tag="PK641_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007759351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCMD domain-containing protein"
                     /protein_id="HPG55191.1"
                     /translation="MYMKLKPILSLGLIAYFLPILAEEKPIQFGDFEQWATREIKESA
                     IIGGKTKQIYVIAPDEYIKGNIPYKYGVKTPWSSSNAYAKVAGIHKTSNSVSPEKRGN
                     GRCARLDTKMEYVKVLGMIDISVLVSGSIFLGETLEPITGTSDPYGFINMGVPFTQKP
                     KALVMDYKCIVSQNTFVMKHPGIGSTRIEGKQDRAEFILYLQKRWEDADGKIYAKRIG
                     TIRHQLTSDVPEWQNNARFPIQYGDISKTPYYKPFMALSGSNGPFKAPNKKGKMVLVR
                     EICWGDEDDVPTHMILMITSGNQGAFIGTVGNTLWVDNIKLDY"
     gene            complement(9149..>9525)
                     /locus_tag="PK641_02155"
     CDS             complement(9149..>9525)
                     /locus_tag="PK641_02155"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPG55192.1"
                     /translation="MKDVWNLPAIAPWEKSCGKHPTQKPLALLTRIILASTEKNGWIL
                     DPFTGSSTTGIAANIANRKFLGIDKVEEFLSISKNRKLEIENPQIFGTYRKKLGGFED
                     KKQLKLFLATEPEAEYQRTLNF"
BASE COUNT         3005 a         1833 c         1513 g         3174 t
ORIGIN      
        1 taaatctctt aaattccgag cgacagctac cacctcctta ttttcaggat tgataaaatt
       61 aaaatcaccg aaaccgaaat tagtagttac ggcatccttc aactcaagat gcattgtttc
      121 attattttta ttaataaaat aggccccaag tttttcggca agaggcttgt ttttatcctc
      181 acttgattct ataattatag gcaagaactt gtctttagat cttatgtacg aacataaatt
      241 gattccggcc gcaccatcca ttacaccatt acgcggaaat cttacatcag atactacacc
      301 aagaatattt tttgagtatt tatcataaag tttcaatcct tcttcataac tacgagccaa
      361 taaaatcttt ggcctaccac gcattcgcaa cattctttca tgatcattta atgcctctgt
      421 cataaacgac ctagattgca caaatacatg tttatataga tatggcaata tagcagaata
      481 aaaatgtata gagtcttcaa taaacaaaat tgcttgaact cctactgatg cgatatcctt
      541 atcaacattc atcttgtctt caatcaactt aacaattgcc aacaaaagat ctgtttcgcc
      601 taaccaacta aatacataat caatgcccgt aaaatcctca tgtccgaaaa ccttcttaaa
      661 catttcacgc gaaaagggag ttaataacac aatgggaata gatggccttt ggcttttgaa
      721 ttcttttgac agctcgaata cctcactatt gtcgccggaa ggcataacaa ttataagttc
      781 gaaagacctg gcttttagta aatccttagc atctttcatt gttgatgcta aagtaaaacg
      841 aggaggataa cgaagattta gtgaagtata ctcattaaat atctgctcat ctattctacc
      901 atcttcttca agtgtaaacg catcatactt actagcaact agcaagatat tatagatacg
      961 ttgagtcatt aaacttgcaa aagaagtatc tttaaagtat agtttaatcc tatcaggtct
     1021 attcataatg gcaaatatac actttttcta tcgcttgcga aatgcagttc ctctaaaaac
     1081 tgcaattaat tgatctgtgt cttccattct tacttcaaca tcgtacaaaa ttatagtttt
     1141 accttgtttt acgggttttg caatagatat taaaactcct tcagatttgc ctctgaaata
     1201 atctatttgc gcattcaatg tgacgcattc tccgcccatc gaatttgaag cagcccccat
     1261 tgtataatct gccaaagtaa ataaagcacc tccttgaaca gatcccaagc cgtttaaatg
     1321 ctttttagtt attggcattc tcatctttgc atatccatta taagcttcaa gaatctcaat
     1381 tcctatcgat tttgcaaact catctttttg aatgtggttt tctactatca tatattcaat
     1441 tataaaaaaa gcccctatag caactaacta caagggcttt ttatggtttt gaactaaaat
     1501 ttatttatga aaataaaagt cgcaacgacg attcttatat tctttcattg gagggttttg
     1561 catttttccc tttggtgtta cagaaattct agaagcatct ataccatatt tttctatcaa
     1621 ctctttctta actgtttcaa cacgattgct acttaacttc atattataat cgccacttcc
     1681 cgggaaatcg caatagccac gtaattcaac ttgtaactca ggatcttctt ttaatttctg
     1741 agcagctttt gctacattgc tcttagcaac attagtcaaa taatatttgt cgaatgggaa
     1801 atatacagac atttcataat caatgccggc acccattgca gctaatacgc ttttaacctc
     1861 atcacaacat tttgcagatt gcataatttc ttgtggaata ctttcacccc aatagtttac
     1921 gaaagatcct tgtggggtat taggttcacg atcacggaaa tcaggaactc catcaccatc
     1981 accatcaggt ctggttgctt gttgagcttc caaagcagct atacgtggta aaacattatt
     2041 gccaagagta tcttcaacag cagctacacg ttttttcaaa ctatccaatt gatcttggaa
     2101 gtttttgtcg ccggttctta attcacgata agcataagga cttatgttac gcatatgtcc
     2161 accctctttt ttgttaggag caaactttac acgaaaattt aaagtagctg taaattcacc
     2221 gtcattcata gttcctttgg tgtatgcttc accttcaaaa taatctttat tatggaaacg
     2281 ataatgcgcg ttcaaaccta aggctaaata tttgttgaag ttatattcaa cgtttaatcc
     2341 tacagggaaa catatagcca taccatctgt tatatcctga ggaccggctg cagctgctgc
     2401 agctgcgatt tcagccggag ttttaccttc attagtagcc ctagttattg cagcctgttc
     2461 gacagttcct atcggagcac ccggaatagg agttgtttgc atatcgtaaa aagttaaacc
     2521 caaaccggcg ttcaaataaa atccccattt ccaagtgcta cgatattgcc caaacaagtt
     2581 caaaaggttt aacgaagcca taagagttgg ctcatacata ttaccggtaa acgtattact
     2641 accatactcg gttgtttttg tagctcctaa aaatggcata tattggaatt gtgcaatcaa
     2701 accccaatat ggattccatg tgtattcaat atctaatcca actgtccatg aaacagtaga
     2761 ttgagaaaag atatcattat atttttgcgt tacgtcacca tcaaactgag acaatcctcc
     2821 gtttattgat agagaccagt gcgtaaagtc cttaaagtcg tttaatccac ctgttgaatc
     2881 agaaaaagca cttgccgtat ttttagcagc agatgctaag gtaaaactaa acgcaagaac
     2941 tgtgattagt gtcaaaattt ttttcatact cgctttgtta atatatttgg cgcaaatata
     3001 aaacattaat agtatataaa catcatattc tcaatacaaa aggagaaaaa agcttatttt
     3061 ttgagttcat ttgttaaaat tgaaccaaat aaagttgccg gtgatgcttt ttcaatctca
     3121 actatcacat aatcacctct tttcacattc cctttatcaa ataaaactac tttattttgt
     3181 gatgttcttc caaaaagttg ttcgcgggaa cgcttagaat atccttcaac taaaacttca
     3241 aattgtttat caatgtcatt ttcattactc tgaagagata attgcaattg taaatcaatt
     3301 gcttcttgta aacgttttac ttttatttct tccggcacat catccggcaa atgcttggaa
     3361 gcataagtgc cttcacgctc cgaatactta aacataaatg cactatcaaa ggccacttcg
     3421 cgcattaatg ctaaggtttg agcatgatct tcttctgtct ctgaatgaaa cccggtaaat
     3481 aaatctgtcg taataccaca atcgggtata tgtttacgaa tcattgcaat tttatctaaa
     3541 taccactcac gtgtatattt tcgattcatt agttgcaata ttcgattact acccgattgg
     3601 atgggcaaat gtatgtgctt acataggtta tgatgcttag caattgtttt aaccatttct
     3661 tcatccatgt ctttaggatg tgaagtagta aaacgaattc gcatatcagg cacttctccg
     3721 gcaagtttat caagcaattc cgcaaatccc ataccttcaa atttatatga atttacgttt
     3781 tgccctaaca atgtcaattc tttataacct ttttctttta aatctagtac ttcatttaag
     3841 atactttggt attcacgact tctttctcta cctcgtgtat acggtacaat gcaatacgaa
     3901 caaaagttat tgcaaccacg cattattgat acaaaaccgg atattgtatt atgaccgatt
     3961 cgacttggta ttacatcttt gtaggtttga acaactgatg attcaacatt tacaactcta
     4021 cttccttgtt cagcagatgc aattaagttt ggcaaatcca gatatgcatc aggcccaact
     4081 accatgtcta tgacttttct tgataaaaga tcttctttta tcctttctgc catgcatcct
     4141 aatactgcga ctataagttt gttgtttttt ctctttagtg actgaaagaa ctccaaacgc
     4201 gataaagctt tttgctctgc atggtcgcga atggagcagg tgttaataaa gatggcatcc
     4261 gcttcggata tatcttcagt aattacataa cctgccattt gcataactgc tgcaactacc
     4321 tcgctatcag ctatattcat ttggcaacca taggtttcaa gaaacaattt tttttcgcaa
     4381 gcaaatatgg cggatgcata gtccgttggt ttgccatttt ggttttccat atattttttt
     4441 cgttaaaaat gtgcgcaaag atacgctatt ttaatcataa ttcgacaact acataaaaaa
     4501 gcattaaata tttacatata tctgccacta tattgaatag ctataattat tcgttaaaac
     4561 aactatcata taactttaca caaacgtttt cgttaaacta agttaatagt aaagtaaaat
     4621 agtcgcaatc gtttgcaagt taataaaaac attatacctt tgtcaggaga aaatcgaaaa
     4681 gattttttca tagttaaggt ttaggttaaa taaggagagt actgatgttc tctccttatt
     4741 tattttgatg ctaccaatgc aatataattc cgttttctat ttgattattt attatcaaaa
     4801 aattaaaaca gctcattaat aataatatag cttatatggt aaaaaaaaat aaatcaacaa
     4861 tattgtttgt ttattagtac aacttgactt atctttgcca aacatacaaa ggagagtccc
     4921 ggtttacgaa tcgggattct tcttgtttta tctaacaata caaaacaatt atttatatat
     4981 tatggcaaca ccaaattaca tgaccgaaga aggttacaag aaacttatgg aagagatcaa
     5041 ccgacttgaa tcggtagaac gcccaaaaat ttcaaggcaa attgctgaag ctcgcgacaa
     5101 aggagactta tcagaaaatg ccgaatatga tgctgtccgt gaagcacaag ggctactcga
     5161 gttaaaaatc gctcaaatga aggcaacggt ctcaaatgtt cgcatcatag atgaaactca
     5221 acttggcgat ggtacaatac aaatacttag caaagttacc attaagaatg tcaaaaccgg
     5281 ccaaaaaatg acatacacct tagtgtctga agcagaagct aacatacgcg agtttaaaat
     5341 ggcaataagc acacctattg ctaaagctct tataggcaag aaaaaaggcg accacgtaac
     5401 agttgaggct ccttcaggca ttattgaatt tgaaatctta gaagctggat tctaaaacac
     5461 aattatatgg caagcatatt tagtaaaata gtagccggtg aaattccttg ttataaggtt
     5521 gctgaaaccg acaagtactt cgcatttcta gatatcaatc ctttagttaa ggctcatact
     5581 cttgtaattc caaaacaaga agtagattat atttttgatt tatctgatga agatttagcg
     5641 ggcttacata ttttcgcaaa aatcattgct gatgcaatca agaaagcata cccatgcaag
     5701 aaggtgggcg aagctgttat cggattagaa gttccgcatg cacacattca cctgattcct
     5761 attcaaaaag aatcggatat gctgttttca aatccaaaac tcaaattaac agccgaagag
     5821 tttaaagaaa ttgctgcaaa catacaaaat gcaattaaag cataaataat gtgactatca
     5881 aaaaaggggt tcacatctta tgtgagccct ttttttgata tatatttact aaactaaaat
     5941 ttgtgtttat cacaattttg cttctgcttt ttcgatctct tttacaagag acttaggcaa
     6001 attgtcgacg cagtgatgac atttcatttc taaggtgtac atacgtccga tgcaatagtt
     6061 agaatgcttg cagccgttac ggtaatttga atcagattct ttcatcttat ttacaaattc
     6121 aggatcatga actaatgcgc gagccatttg tacaagttca aatcccttat ctaaaacttc
     6181 gtcaatctta gctcttgaaa caagtccacc aacgtagata agaggacatt taatttctgc
     6241 tctaaatttc agtgcaggtt ctagaaaata tgcttcttta aaaggaacgg taggaatcat
     6301 ttgtctgcca acaactttca atcctgcttt taaccaccag aatttccacg gattcatata
     6361 atgtcctaag gttttaagtg gcatagctcc acacataaca tgcataggtg ctttgcttac
     6421 aaatccggcg ctaagtacaa ttccgtgtac accgcatttc tctatttcct ttgcaacggt
     6481 caagcagtcg ggaagttcta atcctccttt aaatccatca tacatattgg tttttacgac
     6541 tactgccatg tcgtctttag cgtgctgcat tacttcgtgt aaaacttggc gcataaaacg
     6601 catacgatta tcgaggctgc caccatattc atcttttcta tggtttgtgt atggtgataa
     6661 aaattgagaa atcagataac catgtcctgc atgtatttca acgcaatcaa atccggcttt
     6721 gcggcacata tctacagatt ttccgaaatc ttttaccaaa tcatttattt cagaaatttt
     6781 caaaccacgc ataatggttg gagagtataa attaaatccg cttgaagcac caacaggcat
     6841 cccgccacat gtataatagt gagtcatatt gccgcagtga cctaattgta tgcttgcttt
     6901 agctccttct gcatgtattg catccgttaa tttttttagt tccggaatga tttcctcgcg
     6961 catccataac tgtccgtcaa aagatacacc gttgcggtta acagcgcaat aggcgattgt
     7021 agtcataccg attccgcctt tggctacgct tgtatggtag ttaaacaaat cttgtgaagg
     7081 cttgttatca taacacatat tttcaaatgc ggccgaacgt atagtacgat tacgtagagt
     7141 aataggtcct aattgaaacg gagtgaataa tttactttct gacatgatga tatgaattgt
     7201 taattatcag tatatgaatg ggaaaacgcg ttttagatgt ttccctttaa attcttgttc
     7261 aaattcaact ttgtattttt tgtaaataga atttgcgcgg ggtactaaat ttgcgcaagt
     7321 ccaccataag aatactgcac ctgcagtcga ccatgttaat attgcaaatc cgccccattc
     7381 taaaatttca ccaaagtaat ttgctgatgt aacatattta tacatgcctt tttgcggtaa
     7441 ataatgatta gtatcacctg gtgcacgaag attccttatt acatggtctg aattccaatt
     7501 tataattaaa cctataataa aaatgcttgt gcctaaaata aattgccagc ttgttaacca
     7561 actttcagtg tacatatttt caggtgcaag ataaaaaatc catcttcctt gtatgtatcc
     7621 gttagcaata ttaaagagta ctcccataag cataatggca ataggcatct tactatctcc
     7681 ttttagtaaa aaagggaaaa taaacgaacg ttgaaaatag tgtaattcga atagtaagaa
     7741 cataattagt ggtacaatct caaatgttct gtctgacatc caccataagt atgctatgac
     7801 aaaaaataca gggcattcca tcatcaccca tgctacttta ttgtttattg acggtcccca
     7861 tttttcactg attaatttgc cgtaacctgc ctcaacaaaa aacaaggata cgaataccac
     7921 caatgctata atagtcatga tgagtaagaa aaaattaaac tgtgctaaat ccataattaa
     7981 tatttattga tgttatgtat aaagtgaatt agttaatatt taattagtag tcaagtttaa
     8041 tattgtcaac ccataaagta ttacccacgg ttccgatgaa tgcaccttga tttcctgagg
     8101 taatcattaa aatcatgtgt gtaggaacat catcttcatc accccaacag atttctctta
     8161 caagaaccat tttacctttt ttgttaggtg ctttgaatgg tccattgcta cctgaaagtg
     8221 ccataaatgg cttgtaatat ggggttttgc tgatatcgcc gtattgaata gggaatcttg
     8281 cgttgttttg ccattctggt acatcactgg ttaattgatg gcgaatagtt ccaatgcgtt
     8341 ttgcataaat ctttccatct gcgtcttccc atcttttttg tagataaagt atgaattcgg
     8401 ccctgtcttg ttttccctca atacgagttg agccgatacc gggatgtttc attacaaagg
     8461 tattctgtga tactatacat ttgtaatcca taactagtgc cttaggtttt tgagtgaaag
     8521 ggactcccat attaataaat ccgtatgggt cgctggtacc tgtaataggt tcaagagttt
     8581 ctcctaaaaa tatgctaccg gatacaagta ccgaaatatc aatcattcca agtactttta
     8641 catactccat tttggtgtca agtcttgcgc atcttccgtt gcctcttttt tccggtgaaa
     8701 ccgaattact tgttttatgg attcctgcaa ctttagcata tgcgttagaa gaactccaag
     8761 gtgtttttac accatatttg taaggtatat tcccttttat gtattcgtca ggagcaataa
     8821 cgtagatttg tttagtcttt ccaccgatta tggcagattc tttaatctcg cgtgtcgccc
     8881 attgttcaaa atcaccgaat tgaatgggtt tttcttcggc taatattggc aaaaagtatg
     8941 ctattagacc taacgataat attggcttta gtttcatata catattttaa cggctgcaaa
     9001 gataattaaa atacattaaa aatcaattta ttaagaaata aatgcattgg gatgttgctt
     9061 gtgaaaattt aaataagaag tgcaatatta ctgttagtaa tgcaattgcg tagcagtcgc
     9121 cgttaatatt attgttcgga gatatttgtt aaaaatttaa ggttctttgg tattcggctt
     9181 caggttcagt tgctaaaaac aattttaatt gttttttgtc ctcaaatcca ccaagttttt
     9241 ttctataagt accaaatatt tgtggattct cgatttcaag ttttctattt ttagaaatac
     9301 tcaaaaattc ttcaacttta tcaattccta gaaattttct atttgcaata tttgcagcaa
     9361 ttccagttgt cgaactacct gtaaatgggt ctaaaatcca accatttttt tcagtagatg
     9421 ctaaaataat tcttgtcagc aatgctaaag gtttttgtgt tggatgtttt ccacaagatt
     9481 tttcccaagg agcaattgcg ggcaaattcc aaacatcttt cattt
//
LOCUS       DAOPID010000032         9462 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_9002_length_9462_cov_4.032954, whole genome shotgun sequence.
ACCESSION   DAOPID010000032 DAOPID010000000
VERSION     DAOPID010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 9462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9462
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9002_length_9462_cov_4.032954"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2182
                     /gene="glgP"
                     /locus_tag="PK641_02160"
     CDS             <1..2182
                     /gene="glgP"
                     /locus_tag="PK641_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HPG55193.1"
                     /translation="GLGVLAGDYLKEASDCNVDMIAVGFLYRYGYFTQTLSMNGEQIA
                     VYEPQMFDTLPIEQLVDEKGEPITIGVPYPGRTVYANLWRVNVGRVSLILLDTDTEKN
                     SEADRTITYQLYGGDNEHRLKQEIMLGIGGIAALKCLGVEKEVYHCNEGHAAFLNVQR
                     LIDLIKDKGLTFNEAMEIVRVSGLFTTHTPVPAGHDKFEEAMFQAYMFDFSSQLGLDW
                     SDFMNMGRETPGDLTEKFSVTVFACNTCQEVNGVSWLHGKVSQNMFKKIWDGYFPEEL
                     HVSWVTNGVHYPTWAVSEWKKLHLKYFGPDFMSDQSNLKHWENIYNVPDEEIWETRKV
                     LKVKLVDYIRSKFSQSWLKNQGDPSRIVNILGKINPDALTIGFGRRFATYKRAHLLFT
                     DLERLAKIVNNPQYPVQFIFTGKAHPADAGGQKLIQNIIEVSRRPEFLGKVIFLENYD
                     MALAKRLISGVDIWLNTPTRPLEASGTSGEKAEMNGVLNFSVLDGWWYEGYRKGAGWA
                     LTDKRTYTNQAHQDQLDAATIYSILENEIIPLYFAKNSKGYSPEWVQYIKNSFAKIAP
                     VYTTKRMLDDYIERFYNKEAKRSKLLQAKNFEKAKEIAAWKEKVSSAWDSIEILDVFT
                     SENFHQVAESGCEYSGSVLIDVKDPELVNSIGVEFISTNLVDGVDKLFKVREMKLTNI
                     EGTKLTFTLKDKVDNGGSFKCAFRMFPKNADLPHRQDFCFVRWF"
     gene            2280..3089
                     /locus_tag="PK641_02165"
     CDS             2280..3089
                     /locus_tag="PK641_02165"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HPG55194.1"
                     /translation="MNEHKLIALDIDGTLAGTDKTISERNINVLDEAQSKGIKVALVS
                     GRPCYGIWPTARKIHLDKWGGYILSFNGGKIVDCKNNNCIYEQVLSHSDLSEICKEVA
                     EYGLNAITYADDVLISDNASNKYVQKESSINKMPVCQVADLVSATDFPTPKCLVVGEP
                     DILEKVEKDMYKRFPNLSVFRSEPFFLEFMPKGVDKAYSLAILLDKLKMCPSQLLSCG
                     DGFNDLSMIKYAGMGVAMQNAQQVVKDAANYICPYTNDEDAVAFVIEKYVL"
     gene            complement(3092..5191)
                     /locus_tag="PK641_02170"
     CDS             complement(3092..5191)
                     /locus_tag="PK641_02170"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HPG55195.1"
                     /translation="MKKNFYIFILILMIASNSALSHNNPFFKPFSNIYQTVPFSQIEI
                     NDYTEGFEAGLEEQSKNIKAIIDNKDKLTFENTIVAIEHADEILGRVANVLFNLTEAE
                     TNDQLDELAQKYSPKLTEASNKIFQNEDLFAKVNYIYQNKASLNLDTEDSMLLEETYK
                     AFIKSGANLSKDDKKKFSDISSNLSKLTLSFGQNVLKETNAYSITITDKTNLLGLPDY
                     ALAAAEQKSKEKGLEGWTFDLSMPSYSSFMRYAENRELREKLYRAYNSKSNKDNQNDN
                     RNIVKQVVNNRLEIAKLMHKTNFAAYKLENTMAQNTDSVYKLLNELLSNYKPTAENEF
                     KTICDFARTYTGDSDFKMQSWDWSFFSEKYKEQNYDLNDGMLKPYFELDRVKQGVFWL
                     AGELYGLQFKHNPSIDVYHPDVKAYEVRDAKNELVAILYTDFFPRASKQGGAWMTEFR
                     GERILNGNRLIPLISIVMNFTKPIGDTPSLLTFDELTTFLHEFGHSLHGMLADTKYAS
                     LSGTNVYRDFVELPSQLMENFATEQAFLDKVGIHYQTGEKIPAELVKKIKDTENYNVA
                     YFCVRQLNFGFLDMAWHTIKETFEGNVEEFEHNACSKTTMFPSIEGTCISTQFNHIFS
                     GGYAAGYYSYKWAEVLDADAYDVFKKHGGIDKTTAESFKNNILSKGGTEHPMTLYKRF
                     RGQEPTIDALLKRNGIK"
     gene            complement(5188..7437)
                     /locus_tag="PK641_02175"
     CDS             complement(5188..7437)
                     /locus_tag="PK641_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HPG55196.1"
                     /translation="MSKEQKEKKENEIIEQAYKNLLNDYLASPHSKKVDIINKAYSFA
                     KNAHKGIRRLSGEPYILHPIAVAHIACKEIGLGSTSICAALLHDVVEDTDYTVDDIRD
                     IFGDKIAQIVDGLTKISGGVFGEQASAQAENFRKLIITMSEDIRVILIKIADRLHNMR
                     TLDSQPTAKQYKIAGETEYIYAPLAHRLGLFSIKSELENLSFKYNFPQDYNFIGNRLI
                     AERNERMAMYDRFSLPIMNKLNSFGYQFIMKKRIKSVYSIWNKMQIKHLPFEEIFDIL
                     GARIVFTPSPGADEKLECWKIYNIITQIYTPHPERTRDWLSTPKANGYEALHVTVMGP
                     DGFWIEVQIRSKRMDDIAEKGLAAHWNYKKDYTSSESKIEEWLNTVNDLLKNPDSDAM
                     HFLDSFKLTLYDAEIFVFTPKGEMKTIAAGSTALDFAFEIHSDIGTHCIGAKVNNKLV
                     PLSYVLKSGDQVEILTAKHQTQSESWLEYVTTAKAKEKLCAILKQSARTVKTKGDEIL
                     RNKLVSLGYNIKKDYPTEIIKRLLDFYKQKSLDSLLTVVGKGELDLVNLSDAVKDKNR
                     RLTIRYWTPSFLRPKAKEEKKPEEIETTINKSTTSETPEERQMSDGDHIIASCCKPIP
                     GDDIIGFINDKGQIVVHKRNCKEALRLKSQRGSKIVTAEWTDKKEQYYTALIEIQGID
                     RKKMLSDIVNVISIKMEANIKKLTVSTLDGIFDAKVNIYVSHVDDIKRLCSEIRKIDG
                     VESVARIEE"
     gene            complement(7450..8373)
                     /locus_tag="PK641_02180"
     CDS             complement(7450..8373)
                     /locus_tag="PK641_02180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HPG55197.1"
                     /translation="MNKFNKICLFLMLLPLTMQAQSDPQISQHLLSKTHYNPAVTGAS
                     SYGNITLIGRHQFVGFSGAPATGLLNFDMLAPSINSGFGLSVVYDRFGPLISYNAMVN
                     YAYHLKTGENQHLSFGLGAGIQVRQYDASGNIYENESDPTEYYDRDTQFAPTVNFGLE
                     YNLKNWVIGASITNIQRYFMKNDLRFPNVNYYLYTRYRFELGRYWDLIPGIAAHYDNC
                     TVNTELNLTVQYIKLFWLGVSYRMSDLFLAESIVPMVGFNITDFVRIGYAYDYNLTQL
                     NQYSKGTHELLLTIRFKTKGDTYKTPRFAEW"
     gene            complement(8390..>9462)
                     /locus_tag="PK641_02185"
     CDS             complement(8390..>9462)
                     /locus_tag="PK641_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPG55198.1"
                     /translation="VNSSFDDINLIVSEPNDEAFLVSSRHPETNDDIYRFEGIFTSMM
                     VSGHVFNKNTQEPIENAQVIINGMSKSQTVQTNNEGYYYAFIEAGDFYKLLASSIGYI
                     SDMSMIKTEKSAIGTFPVEQDFYLSQSAMSISGRVYDLETNEPFINEDVFLLEGSEVI
                     QQTKTDITGRYLFTDLVNGKEYQVKVNKPNFLTISSKPFIYHEGDGENSGFDLASISY
                     NKGNDDPMNRNGRNSNDDGSVSYGREISLHEIYYNFDSANLSPESKDALHRIVLLLNS
                     NPTLKLEFGSHTDMRGTDEYNEKLSVDRAKSVVDYLVGAGINRTRLTWKGYGKRYPVV
                     ENAITEEEHRLNRRTTFKIVEK"
BASE COUNT         2858 a         1584 c         1675 g         3345 t
ORIGIN      
        1 tggcttaggt gttttagccg gtgactattt gaaagaagca agtgattgta atgttgatat
       61 gatagcagtt ggtttcttgt atcgctatgg ctattttaca caaacattgt caatgaatgg
      121 agagcaaata gcagtatacg aaccgcaaat gtttgataca ttgcctattg aacagttggt
      181 tgacgaaaaa ggagaaccca ttacaatagg agtcccttat ccgggtcgta cggtatatgc
      241 taatttatgg cgtgtaaatg ttggtcgcgt tagtttaata ttgttggaca cagacacaga
      301 gaaaaacagc gaagcagatc gtacaattac atatcaatta tatggtggtg ataatgaaca
      361 tcgtttgaaa caagaaatta tgttgggtat tggtggtatt gctgctttga aatgcttagg
      421 cgtagaaaaa gaagtatatc attgtaatga aggacatgca gcattcttaa atgtacaacg
      481 tttaatcgat ttaattaaag ataaaggttt gacatttaac gaagcaatgg aaattgttcg
      541 tgtatcagga ttgtttacaa ctcatacacc ggttcctgca ggtcatgata aatttgaaga
      601 agcaatgttc caagcatata tgtttgattt ctcttcacaa ttaggcttgg attggtctga
      661 ctttatgaat atgggacgcg aaaccccggg cgacttaacc gaaaaattct ctgttacagt
      721 gtttgcctgc aatacatgtc aagaggttaa tggtgtaagc tggttgcacg gaaaagtttc
      781 tcaaaatatg tttaagaaaa tttgggatgg ttatttccct gaagaattgc atgtaagttg
      841 ggttactaat ggtgttcact atcctacatg ggcggtttca gaatggaaaa aacttcatct
      901 gaaatatttt ggtcctgact ttatgtcaga tcaatcaaat ttgaaacatt gggaaaatat
      961 ttacaatgtt cctgatgaag aaatatggga aacacgtaaa gttcttaagg ttaagttggt
     1021 agattatata agatctaaat tctcacaaag ctggttgaag aaccaaggtg atccttctcg
     1081 tattgttaat attttaggca aaattaatcc ggatgctttg acaatcggtt tcggtcgtcg
     1141 ttttgctaca tacaaacgtg ctcacttgtt gtttacagat ctagaacgtt tagctaagat
     1201 agttaacaac cctcaatatc ctgttcaatt tatatttaca ggtaaagctc acccggctga
     1261 tgcaggtggt caaaaattga tccaaaatat cattgaagtt tctcgtcgtc ctgaattctt
     1321 aggtaaagta attttcttag agaattatga tatggctttg gcaaaacgtt tgatttcagg
     1381 tgtagatatt tggttgaata ctcctacacg tccgttggaa gcatcaggta catcaggtga
     1441 aaaagctgaa atgaatggtg ttttgaactt ctcagttctt gatggatggt ggtatgaagg
     1501 atatcgtaaa ggtgcgggtt gggctttgac agacaaacgt acttatacaa atcaagcaca
     1561 ccaagatcag ttagatgctg ctacaatcta ttcaattttg gagaacgaga ttattcctct
     1621 atattttgca aagaatagca aaggatattc tccggaatgg gtacaataca ttaaaaattc
     1681 attcgcaaaa attgctcctg tatatacaac aaaacgtatg ttggacgact atatcgaacg
     1741 tttctataac aaagaagcta agagaagtaa gttgttacag gcaaagaact ttgagaaagc
     1801 taaagaaatt gctgcttgga aagagaaagt ttcatcagca tgggattcta ttgaaatttt
     1861 agacgtattt acttcagaga actttcatca agtggctgaa tcaggttgcg aatattcagg
     1921 ttcagtctta attgatgtga aggatcctga attggtaaat agcataggcg tagaatttat
     1981 ttcaacaaat ttggttgatg gcgttgacaa gttgtttaaa gttcgtgaaa tgaaactaac
     2041 taatatagaa ggaacaaaac ttactttcac tttgaaagat aaagttgata atggtggttc
     2101 attcaaatgt gctttccgta tgttccctaa aaatgctgat ctaccgcatc gtcaagactt
     2161 ctgttttgta cgttggttct aattagtaaa aaacaaacta cattaagata gtcgctgata
     2221 aaaagagttg cttagattta tttgtaagca actctttttt ttaatttcaa gttgagtgta
     2281 tgaatgagca taaattgata gcattagata ttgacggcac attagccggt accgataaaa
     2341 ccatttcaga acgtaatatt aatgtacttg acgaggcaca aagcaaagga attaaagtag
     2401 ctttagtgtc aggcagacct tgttatggga tatggccaac tgcaagaaaa attcatttag
     2461 ataagtgggg tggttatatt ctatctttta atggtggaaa aattgtggac tgtaaaaata
     2521 ataattgtat ctacgagcaa gtgctatcgc attctgattt atctgaaata tgcaaagaag
     2581 ttgccgagta tggtttgaat gcaattactt atgctgatga tgttcttatt tctgataacg
     2641 cttcaaataa gtatgtacaa aaagaatctt cgattaataa aatgcccgtt tgtcaagttg
     2701 ccgatttagt atcagctact gattttccaa ctccaaaatg tttggttgtt ggagaacctg
     2761 atattctaga gaaagttgaa aaggatatgt ataaacgatt cccaaattta agcgtattcc
     2821 gttccgaacc atttttcttg gaatttatgc ccaaaggtgt tgataaggca tattcattag
     2881 caatactttt agataaacta aaaatgtgtc caagtcaatt attgtcttgt ggagatggtt
     2941 ttaatgattt gtcaatgata aagtatgccg gaatgggagt tgctatgcaa aatgctcagc
     3001 aagttgttaa agatgcagca aactacatat gcccttatac aaacgatgag gatgcagttg
     3061 cattcgttat tgagaaatac gtcttgtaat tctattttat tccgttcctt ttgagcaaag
     3121 catcaattgt aggctcttga cctctgaatc gtttgtatag tgtcatggga tgctctgtgc
     3181 cacctttgct gagaatgtta ttcttgaatg attctgcagt tgttttatca attcctccat
     3241 gttttttaaa tacatcgtat gcgtctgcat ctaatacctc agcccatttg taactgtaat
     3301 atcctgcagc ataaccaccg gagaagatat ggttaaattg agtgcttata caagttcctt
     3361 cgatactggg aaacattgtt gttttactac atgcattatg ctcaaattct tctacgtttc
     3421 cttcaaaagt ttcttttata gtatgccatg ccatgtctaa gaaaccaaaa tttaattgtc
     3481 taacgcaaaa gtatgctaca ttgtaattct ctgtatcttt aattttcttt acaagttcag
     3541 ctggaatttt ttcgcctgtt tggtaatgta tacctacttt atctaagaaa gcctgctctg
     3601 ttgcaaaatt ctccattagt tgtgatggta gctcaacgaa gtctctatat acatttgttc
     3661 cgctcaaact agcatatttt gtatctgcca acattccatg taaagaatgg ccgaactcat
     3721 gtaaaaatgt agtaagctca tcaaaagtca aaagagaggg tgtatcgcct attggctttg
     3781 tgaaattcat gacaatggag ataagtggaa ttaatctatt cccatttaat atacgttcgc
     3841 ctctaaattc agtcatccat gctccacctt gtttgctcgc gcgtggaaaa aaatctgtat
     3901 ataatatggc aactaattca ttttttgcat ctctaacttc gtatgctttc acatcagggt
     3961 gataaacatc aattgatggg ttatgtttaa actgcaaacc atataattca ccggctaacc
     4021 agaatacacc ttgtttaacg cgatccaatt caaaatatgg tttaagcatt ccgtcattta
     4081 aatcataatt ctgctcttta tatttttctg aaaagaatga ccaatcccat gattgcattt
     4141 taaaatccga atcgcctgta tatgtccttg caaaatcaca tatagtttta aattcatttt
     4201 cggcagttgg tttgtaatta gataatagct catttaaaag tttgtacacg ctatctgtgt
     4261 tttgggccat tgtgttttct aacttatatg cagcaaaatt agttttatgc atcaacttgg
     4321 caatttctaa gcgattgttg actacttgtt taactatgtt tctgttatcg ttctgattat
     4381 ctttatttga tttagagttg tatgcacgat ataacttttc gcgtaattct cggttttctg
     4441 cgtatcgcat aaaagaagag taacttggca tacttaaatc aaatgtccag ccttctaatc
     4501 ctttttcttt agatttttgt tctgcagctg ctaatgcata atcaggtaat cctaaaagat
     4561 ttgttttatc tgttattgta atgctgtatg cgttagtttc tttaagaacg ttttgaccaa
     4621 atgataatgt aagtttactt agattactac ttatatcaga gaatttcttt ttgtcatctt
     4681 tagataaatt tgcgcccgat tttataaatg ccttataggt ttcttccaaa agcatagaat
     4741 cttcagtgtc aagatttaag cttgctttat tttgatatat gtaattgact tttgcaaaca
     4801 aatcttcatt ctgaaatatc ttattggagg cttctgttaa ttttggagaa tatttttgtg
     4861 ccaattcgtc taattgatca ttggtttcag cttctgtaag attaaaaagt acatttgcaa
     4921 ctctgccaag tatttcgtct gcgtgttcaa tagctacaat agtattttca aatgtaagtt
     4981 tgtctttatt gtcaataatt gctttaatat tcttgctttg ttcctctaaa ccggcttcaa
     5041 aaccttctgt ataatcattt atctcgattt gactaaacgg aaccgtttga taaatattac
     5101 taaatggttt aaaaaaagga ttattgtgcg acaatgctga attgcttgca atcataagta
     5161 tcaaaataaa tatataaaag ttttttttca ttcttctatt cgggcaacac tttcaactcc
     5221 gtcaatcttt cttatttctg agcaaagtcg ttttatatcg tccacatgac taacatatat
     5281 attgactttt gcatcgaata taccatctag ggtagaaact gtaagtttct ttatattagc
     5341 ttccatcttt atggaaataa catttacaat gtcagatagc attttctttc tgtcaatacc
     5401 ttgaatttca ataagtgcag tatagtattg ttctttttta tcagtccatt ctgctgtaac
     5461 aattttagaa cctctttgtg attttagacg taaggcttct ttacaattgc gtttgtgaac
     5521 aacaatctgt cctttatcat taataaagcc aataatatca tctcccggaa taggcttgca
     5581 acatgatgca ataatatggt ctccgtcaga catctgacgt tcttcaggtg tctcactagt
     5641 tgttgatttg tttattgttg tttcaatttc ttccggcttt ttttcctctt tggctttggg
     5701 tctcaagaag ctaggtgtcc aataacgtat agttaaacgt ctgttcttgt ctttaaccgc
     5761 atctgataga tttactaaat caagttcccc ttttcctaca actgttaaaa gtgagtctag
     5821 tgatttttgt ttataaaagt ctagaagtcg tttaataatt tcagtcggat aatctttttt
     5881 tatgttatat cctaatgaaa ctagtttatt gcgaagaatt tcatccccct tagtcttaac
     5941 tgtcctggct gattgtttta atatggcaca caatttttct tttgcctttg cagtagtaac
     6001 atactcaagc catgattcac tttgtgtttg gtgcttagct gttaaaattt ctacttgatc
     6061 tccacttttt aatacatagc ttaatggaac taatttattg ttgacttttg caccaataca
     6121 atgcgtacca atgtcagaat gtatttcaaa tgcaaaatct aaagcggtag atccggctgc
     6181 aatggttttc atttcacctt ttggagtgaa tacaaaaatc tctgcatcat ataaagttaa
     6241 tttaaatgag tctaaaaaat gcatggcatc agaatccgga tttttaagta agtcatttac
     6301 cgtattaagc cattcttcta tctttgattc gctggaagta tagtcttttt tataattcca
     6361 atgtgcagct agtcctttct ctgcaatgtc atccatcctt ttacttcgga tttgaacttc
     6421 aatccaaaat ccatcaggtc ccatcacggt aacgtgcaga gcttcatatc cattggcttt
     6481 tggtgtgcta agccaatctc ttgtacgttc ggggtgtgga gtataaatct gtgttatgat
     6541 gttgtatatt ttccaacatt caagtttttc atctgcgcct ggtgaaggtg tgaatacaat
     6601 acgtgcacct aaaatatcaa atatttcttc aaatggaaga tgtttaattt gcattttatt
     6661 ccaaatcgaa tatacagatt ttattcgttt tttcattatg aattgataac cgaagctatt
     6721 caatttattc ataataggaa gagaaaaacg atcatacata gccatacgtt cgtttctttc
     6781 tgcaattaaa cgattcccaa taaaattgta atcttgtgga aaattatatt tgaaacttag
     6841 attttctaat tcagatttta ttgaaaaaag gcctaatctg tgagcaagtg gggcatatat
     6901 gtattcagtc tcaccggcaa ttttatattg tttggcagta ggttgcgaat ctaatgttcg
     6961 catgttatga agtctatcag caatctttat tagtatcact cgaatgtctt cagacatagt
     7021 gataatcaat tttctaaaat tttcagcttg tgcagatgct tgctctccaa aaactccgcc
     7081 ggatattttt gttagtccat ctacaatttg tgctatttta tctccgaaaa tatctcttat
     7141 atcgtcaacc gtatagtcgg tatcttctac aacgtcatgt agtaaagcag cacatattga
     7201 agtagaacct aatccaattt ctttacatgc aatatgagca actgcaatag gatgtaaaat
     7261 ataaggttct ccggaaagcc ttctaatgcc tttatgagca tttttagcga atgagtaggc
     7321 tttattaata atatcaactt ttttgctgtg tggcgacgct agataatcat tgagcaggtt
     7381 tttataagcc tgctcaatga tttcattttc ttttttttcc ttctgctctt tagacacagt
     7441 atcaaaacat taccattcag cgaaacgagg tgttttatag gtatcacctt tcgttttgaa
     7501 gcgaattgtt aataataact cgtgagttcc tttgctatat tggtttaatt gagttaagtt
     7561 ataatcgtat gcataaccta tgcgcacaaa atctgtaata ttaaaaccaa ccattggaac
     7621 aatagattct gctaagaata agtcactcat tctataagat acacctaacc aaaatagttt
     7681 gatatattga acagttaagt ttagttcggt attaacagtg cagttgtcgt aatgtgctgc
     7741 gatacctgga attaaatccc agtagcggcc tagttcgaaa cgataacgcg tgtataagta
     7801 gtaatttaca ttcggaaaac gtaaatcatt cttcataaaa tagcgttgta tatttgtaat
     7861 agaagcacca ataacccaat tttttagatt gtattccaat ccaaagttaa cagttggtgc
     7921 aaattgagtg tctctatcgt aatattcggt aggatcactt tcattttcgt aaatattacc
     7981 gctggcatca tactgacgaa cttgtatacc ggcacctaga ccaaacgaca aatgttgatt
     8041 ttcgcctgtt tttagatggt atgcataatt taccattgca ttatatgaaa tgagtggtcc
     8101 aaaacggtca tatactacag ataacccaaa tcctgaattt atacttggtg caagcatatc
     8161 aaagtttagt aagcctgttg caggagctcc actaaatcca acgaactggt gtctacctat
     8221 taaagtaata ttaccatagg aagatgctcc tgttactgcg ggattataat gagttttact
     8281 taaaagatgc tggcttatct gtggatcgct ttgagcttgc attgttaagg gtagcaacat
     8341 caaaaacagg catatcttat taaatttatt catggtgctg tattttaggt tatttttcaa
     8401 ctattttaaa tgtggtgcga cgattcaatc tgtgctcttc ttctgtaata gcattttcaa
     8461 caaccggata tcgcttgcca tatcccttcc atgttaagcg tgttctatta ataccggcac
     8521 caaccaaata atcaacaact gatttggctc tgtctacaga tagcttttca ttgtattcat
     8581 cagtacctct catatctgtg tgtgaaccaa attccaattt taaagtagga tttgaattaa
     8641 gaagcaatac gattctatgt aaagcatctt ttgattcagg agataagttt gcactatcga
     8701 aattatagta aatttcatgc aatgaaatct cgcgtccgta agatacagat ccgtcatcat
     8761 ttgaattgcg accattccta ttcataggat cgtcattacc tttgttataa gagatggatg
     8821 caaggtcgaa accactattt tctccatcgc cttcatgata tataaaaggt ttactgctaa
     8881 tagtcaagaa gtttggtttg ttaaccttaa cttgatactc tttaccattt actaagtcag
     8941 taaacaagta gcgacctgta atgtctgttt ttgtttgttg aataacttca cttccttcta
     9001 gtaggaaaac gtcttcatta ataaatggtt cgtttgtttc caaatcgtat acgcgaccac
     9061 ttattgacat tgccgattgt gataaataga aatcttgttc aacagggaaa gttcctattg
     9121 ctgatttttc tgttttaatc attgacatgt ctgatatata tcctatagag ctagctaata
     9181 atttgtagaa atctccggct tcgataaatg cgtaatagta tccttcattg tttgtctgta
     9241 ctgtttgaga tttgctcatt ccatttataa taacttgtgc gttttctata ggctcttgag
     9301 tgtttttatt aaatacgtga ccggaaacca tcatacttgt gaagattcct tcaaaacgat
     9361 aaatgtcgtc atttgtttca ggatgtctac tactaaccaa gaaagcttca tcgttaggtt
     9421 ctgacacaat caaattaata tcatcgaacg atgaattcac ag
//
LOCUS       DAOPID010000033         9323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_9216_length_9323_cov_3.976586, whole genome shotgun sequence.
ACCESSION   DAOPID010000033 DAOPID010000000
VERSION     DAOPID010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 9323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9323
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9216_length_9323_cov_3.976586"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="PK641_02190"
     CDS             <1..446
                     /locus_tag="PK641_02190"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPG55199.1"
                     /translation="IRHYGELVLGEVLLVVEYVILIHGLGHDHSLTDGDNCHHHTKPK
                     AKGIYGAFLLGVLFALAFCPETAIFYFGMLIPLSFESTIGYTLPFIYAVITALPVLFF
                     AGIISYGTDKSTKYLNKIQKIQKWLTAILGALFTIMGIWLLIENI"
     gene            579..1598
                     /gene="recA"
                     /locus_tag="PK641_02195"
     CDS             579..1598
                     /gene="recA"
                     /locus_tag="PK641_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPG55200.1"
                     /translation="MATEEKKEAQKPNLEKLKALQLAIDKIEKDHGKGTIMKLGDDHV
                     VDVPVIPTGSIGLNAALGVGGLPRGRVIEIYGPESSGKTTLAIHAIAQAQKVGGIAAI
                     IDAEHAFDRFYAAKLGVDVENLFISQPDNGEQALEIADQLIRSSAIDIIVIDSVAALT
                     PKAEIEGDMGDSKMGLQARLMSQALRKLTANINKTNTCCIFINQLREKIGVMFGNPET
                     TTGGNALKFYASIRLDIRKIGQIKEGDEVIGNQTRVKVVKNKVAPPFRKAEFDIMFGE
                     GISRSGEIVDLGVEAGIIKKSGSWFSYGETKLAQGRDATKKLISDNPELSAELEQKIM
                     EYLKK"
     gene            1600..2328
                     /gene="rlmB"
                     /locus_tag="PK641_02200"
     CDS             1600..2328
                     /gene="rlmB"
                     /locus_tag="PK641_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HPG55201.1"
                     /translation="MNDLIFGLRAIIEAIEAGKEIDKILLKKDMQSDLAKELFAAVKG
                     RNIPVVRVPVERIDRITKKNHQGAIAFISPITYQRITDIIPSLYEAGKSPLIVVLDGV
                     TDVRNFGAIARTCECAGVDAIVLPQSGSAPVNADAIKTSAGALMSIPVCREHNLAYVL
                     QFMKDSGIKLIAASEKAAKDYTLLSYTEPTAIVMGAEDIGISQSILALCDESARIPIL
                     GEIASLNVSVAAGILIYEAVRQRK"
     gene            2335..3015
                     /locus_tag="PK641_02205"
     CDS             2335..3015
                     /locus_tag="PK641_02205"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPG55202.1"
                     /translation="MTILYEDNHLIAVNKTCNEIVQGDKTGDEPLVETVKKYLKEKYA
                     KPGNVFCGVTHRLDRPVSGVVVFAKTGKALERFNEMLRNREIKKNYWAIVKNPPKEPE
                     KRLEHYLVRNEKQNKSYAYEEPKGDAKKAILSYKTIAKSDNYTLLEVDLETGRHHQIR
                     CQLAHIGCPIKGDLKYGFDRSNKDGGISLHARKIEFIHPISKQAICIIAPVPQDSLWQ
                     AIESMLSK"
     gene            complement(3116..4078)
                     /locus_tag="PK641_02210"
     CDS             complement(3116..4078)
                     /locus_tag="PK641_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006283365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPG55203.1"
                     /translation="MNAGTIVLLILVLLVVIYVMRGFKIVSQSETMIIERLGKYQRTI
                     TAGINIILPIVERAKDTITRMPNGSLRMNSRIDLREQVYDFDKQSVITKDNVMTEINA
                     LLYFQIVDPMKSVYEIQNLPLAIEKLTQTTLRNVVGEMELDETLTSRDTINTKLRNVL
                     DEATNKWGVKVNRVELQDITPPESIRFAMEKQMQAERDKRAEILKSEGEKQSAILKSE
                     GEKSAEINAAEAEKQAKILKAQGHAEAQILQAEAEAKAIRKISEAVAAQQGANPVNYL
                     LAIKYIDGLKEMVSGKDNKTIYLPYEATGVLSSLGGIKDMFKNQ"
     gene            complement(4083..4511)
                     /locus_tag="PK641_02215"
     CDS             complement(4083..4511)
                     /locus_tag="PK641_02215"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HPG55204.1"
                     /translation="MEPYHYWLIAAILLTILEIFTATFSVFCFAIGCVFAVIAALCGL
                     DLNWQVLSLIIGTVLGFVLVRPFALKFLNRKKDIATNADALMGRIAIVSETIETSKGT
                     GRVVVDGDDWKAITLDDELIEKGEKVKIVGRNSIILTVKK"
     gene            4709..6712
                     /gene="pulA"
                     /locus_tag="PK641_02220"
     CDS             4709..6712
                     /gene="pulA"
                     /locus_tag="PK641_02220"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HPG55205.1"
                     /translation="MKNTIQVVLIFFIAIFMTSFDFPAHYNSFDNYPIYNDNDLEVTY
                     TPSKTTFKIWSPDIDSAKVRLYKTGIEDDPIETMLMIKKEDGIWEVSADGDLKGLFYT
                     FQVQFENEWLRESPGSYAKAVGVNGFRGAIVDMQETNPEGWENDKRPAQKTFNDIMLY
                     EVHVRDFSMAANSGMKNKGKFLAFTEEGTVNSFGQKTGIDHLKELGITHIHLLPSFDY
                     ASINETKLKENKYNWGYDPSNFNVPEGSYSTNPYDPICRIKEFKQMVQSLHKNGIRVI
                     MDVVYNHTFKCEISNFNMITPGYYYRHNADGSFSNASGCGNETASERAMVRKYIIESV
                     KYWATEYHVDGFRFDLMGIHDIETMNLTRAALNEIDSTILIYGEGWTAGDSPLPYEQR
                     AVKQNANQMDGIAVFSDDLRDALKGSWANAREPGFACGKQGYDETIKFGVIGATQHDQ
                     IHYGLINYSNAPFANQPSQVINYVSCHDDLCLNDKLSYSAPEGATPQELKKFNKFAQS
                     IVFTAQGIPFIYAGEELMRNKKGIHNSYQSPDSINQIDWDFKKTNESIYQYYKSLILL
                     RKNHPAFRMSTTKMIQDNLHFLDPGLACVVAYTIDNNANGDSWNRILVVHNGNRHEIS
                     LDIPKENWWVISNGEIINQNGIFKFNKEYLTVPASSTMILCKP"
     gene            6700..7482
                     /locus_tag="PK641_02225"
     CDS             6700..7482
                     /locus_tag="PK641_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HPG55206.1"
                     /translation="MQTLTSSDIAKYIEEYAPLSLQEEWDNCGWQLTNGDAIVSGVML
                     CVDITESVIHEAIEKDCNFILSHHPLIFDGLKKITDEPISRCVRTAIEHNIALYSAHT
                     NIDKVFNGVSAGMCKKLKLNNCKILESDCTNSGFGMIGTLNKPISCTDFLKQLQYTFN
                     PICLRHSQITDMAIKTVAVCGGSGSFLISRAKELCADIFVTADLKYHDFFKADERFIL
                     ADIGHFESEQYTKEIFFEILSKKMPTFAILNSEKEHNPIYCL"
     gene            7494..8264
                     /locus_tag="PK641_02230"
     CDS             7494..8264
                     /locus_tag="PK641_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HPG55207.1"
                     /translation="MAEKTQDAKEISVEQKLKALYDLQTIVSDIDKIKILRGELPGEV
                     QDLEDEIIGLQTRLKNIETEITNINAEIAKRNQNIIDSNALIARYKEQMDNVRNNREY
                     DHLTKEMEFQSLEIELAQKNIRDYSVAIERKHEEFEKSKYLLEDKTIVLNEKKEELDE
                     IIVETKQEEERLREDAKKIETIIESRLLQAFKRIRKNAHNGLAVVYVQRDACGGCFNK
                     IPAQRQLDIRLRKKIIVCEHCGRILIDPELAGVVEESK"
     gene            complement(8314..8958)
                     /locus_tag="PK641_02235"
     CDS             complement(8314..8958)
                     /locus_tag="PK641_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HPG55208.1"
                     /translation="MAENKIIISIGRQFGSGGRVIGRMLAEYFNIPYYDKELIKCASE
                     DSGIKDEFFKSADERQSGGLLSALNLDWSLGSMFVPSGQYNLLSGESLFKFQSETIKR
                     VAQNSCVIVGRCANYVLRDDPALFSVFITADIETRVKRVLNLKEYDIKGENIESLVKK
                     ADKSRASYYNYYSNTEWGVASTYDLCINSSVCGIEKTAEIIADVIKITHFRNKA"
     gene            complement(8969..>9323)
                     /locus_tag="PK641_02240"
     CDS             complement(8969..>9323)
                     /locus_tag="PK641_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPG55209.1"
                     /translation="ICQCFPTFVVSLFTRDPQLIEISKHALHIITFIFPVVGYQMVAT
                     NFFQSVGLAKKAIFLSLNRQMLFLIPFLLVLPRFFGIEGVWWSMPMADFVSTIVVSIL
                     LMNQIKQFKAEYQNN"
BASE COUNT         3319 a         1700 c         1624 g         2680 t
ORIGIN      
        1 acattcgtca ctatggagaa ttggtgttgg gcgaagtgct tcttgttgtg gaatatgtga
       61 ttttaataca tggactaggt cacgatcata gtcttacaga tggagacaac tgccaccatc
      121 acacaaagcc taaagctaaa ggaatttatg gagcattttt acttggagtt ttatttgctt
      181 tggctttttg ccctgaaaca gccatttttt acttcggaat gcttattcct ctatcgttcg
      241 aatcaacaat aggatacaca ttaccattta tttacgccgt aataaccgca ttgcctgtct
      301 tgttttttgc gggtataata tcatatggaa ccgacaaatc aacaaaatac ctcaataaga
      361 ttcaaaaaat acaaaaatgg ctcacagcta ttttaggggc attatttact attatgggta
      421 tctggctgtt gatagaaaat atttgacaaa aaatttgttg ataaattttt atcaacaatc
      481 gaacagtcat acatagttgg gcaataaaat tgtgtatctt tgcagataat cttaatatta
      541 atctaacgga gatactctcc tttaaaaaac aacaatttat ggcaacagaa gaaaaaaaag
      601 aagcgcaaaa gccaaacttg gagaaattaa aagctttaca attagcaatt gacaaaattg
      661 aaaaggatca tggtaaaggt actatcatga aacttggcga tgaccacgta gtagacgttc
      721 cggttatacc aacaggatct ataggactta atgctgcatt aggagttgga ggattgccac
      781 gtggccgcgt tatcgaaata tatggacctg aatcatcagg aaagacaaca ttagcaatac
      841 atgctattgc acaagcccaa aaagtaggtg gtatagccgc tattattgat gcagaacacg
      901 cttttgatcg tttctatgca gcaaaattag gcgttgatgt agaaaatctt ttcatatcac
      961 aacctgacaa tggtgaacaa gcactcgaaa ttgcagacca actaatacgt tcatcagcaa
     1021 tagacattat agtaatcgac tcagttgcag ctctaacacc taaagcagaa attgaaggtg
     1081 acatgggtga ttcaaaaatg ggattacaag cacgattaat gtcacaagca ttacgtaaac
     1141 ttacagcaaa catcaataaa acaaatactt gctgtatttt tattaaccaa ttacgcgaaa
     1201 aaataggagt tatgttcgga aatccggaaa cgacaaccgg tggtaacgcc cttaaatttt
     1261 acgcatcaat ccgtttggat atccgcaaaa taggacaaat caaagaagga gatgaagtta
     1321 taggaaatca aacccgcgta aaagttgtaa agaacaaagt tgctcctcct ttccgcaaag
     1381 cagaatttga tatcatgttt ggcgaaggca tctcacgcag cggtgaaatc gtagacttgg
     1441 gtgtagaagc aggaatcata aagaagagcg gttcttggtt tagctatgga gaaaccaaat
     1501 tagcacaagg tcgcgatgca acaaagaaac taataagcga caatcctgaa ttatctgcag
     1561 aattggagca aaaaataatg gaatacctaa aaaaataaaa tgaacgacct gatattcgga
     1621 ctacgcgcca tcatagaggc tattgaagcc ggaaaagaaa ttgataaaat acttttaaag
     1681 aaagacatgc aaagtgactt ggcaaaggag ttatttgcag ctgttaaagg acgtaatatt
     1741 ccggttgtac gcgtacctgt cgaaaggata gaccgcatta caaagaagaa tcatcaggga
     1801 gctatcgcat ttatttcacc aataacttat caaagaatca cggacataat tccctcctta
     1861 tacgaggcag ggaaaagtcc gttgattgtt gtattagacg gagttacaga cgtacgtaac
     1921 tttggagcaa tagcacgtac ttgcgagtgc gccggagttg acgcaatagt gcttccacaa
     1981 tcaggaagtg ccccggtaaa tgcagatgca ataaaaacat cggcaggagc attaatgagc
     2041 attcccgtat gtcgtgaaca taatttagca tacgtactgc aatttatgaa agattccgga
     2101 atcaaactta ttgcagcatc cgaaaaagca gcaaaagact atactctact atcatacaca
     2161 gagcctacag ccattgtaat gggtgcagaa gacataggaa tatcgcaaag tattctagcg
     2221 ctttgcgatg aatccgcacg tattccaata cttggcgaaa tagcatcatt aaatgtatcg
     2281 gtggcagcag gtatacttat atacgaagca gtaagacaaa ggaaataata ttctatgact
     2341 attctgtacg aggacaatca tcttatcgct gtaaataaaa catgcaacga aatcgttcaa
     2401 ggggacaaaa ccggagacga gccacttgtt gaaactgtaa aaaaatactt gaaagaaaag
     2461 tacgcaaaac ccggaaatgt tttttgcggt gtaacacaca gattagacag acctgtaagc
     2521 ggtgttgttg tttttgctaa aacaggaaaa gcacttgaac gttttaacga gatgctccga
     2581 aatagagaaa tcaaaaagaa ctattgggct atcgtaaaaa accctcctaa agaacctgaa
     2641 aaacgcttgg aacattactt ggttcgtaac gagaaacaaa ataaatctta cgcatacgaa
     2701 gaacccaaag gggatgcaaa gaaagcaatc ctttcgtaca agacaattgc gaaatcagac
     2761 aattataccc tactggaagt tgatttggaa accggcagac atcatcaaat acggtgtcaa
     2821 ttagctcaca taggctgccc tatcaaaggc gatttaaaat atggtttcga cagatcaaat
     2881 aaagacggag gaattagcct gcatgcacgt aaaatagaat ttatccaccc tatcagcaaa
     2941 caagcaatct gcataatagc acctgttccg caagactcac tgtggcaagc catagaatca
     3001 atgcttagca aataagcaaa ctccattaga atacaaaaaa actattgatt tacataataa
     3061 ctaaacagcc gaagtacaaa ccccggctgt ttagttatta tatatataat ttactttatt
     3121 ggtttttaaa catatccttt ataccaccaa gcgaacttaa tacacctgta gcctcatacg
     3181 gcaaatatat tgtcttattg tctttcccgc taaccatttc ttttaaaccg tcaatgtact
     3241 taattgccaa caagtaatta accggattag caccttgttg agcagctaca gcctcagata
     3301 ttttgcgaat tgcttttgcc tctgcttctg cctgaagaat ttgagcttcg gcatgacctt
     3361 gggctttcaa tatcttagct tgtttttcag cttcagcagc attaatttct gcagatttct
     3421 caccttctga tttcaatatt gccgattgtt tttcaccttc tgactttaat atttctgcac
     3481 gtttatcacg ctcagcttgc atttgttttt ccatagcaaa acggatgctc tcaggtggtg
     3541 taatatcctg caattcaaca cgattaacct ttacgcccca tttattggta gcctcatcaa
     3601 gaacattacg caattttgtg ttaattgtat cacgcgatgt taatgtctca tctaattcca
     3661 tttctccgac tacattacgc aatgtagttt gagtcaattt ttcgatagcc aaaggcaaat
     3721 tctgtatttc atatacagat ttcataggat caacaatctg gaaataaagc aatgcattta
     3781 tttctgtcat tacattatct ttagtaataa cgctttgttt gtcaaaatca tacacttgct
     3841 cacgtagatc tatacgcgaa ttcattcgta aagatccatt aggcatacga gtaattgtat
     3901 ctttcgctct ttctacaatt ggtaaaatga tattaatacc ggcagttatt gtacgttgat
     3961 attttcccaa tctttcaatt atcattgttt ccgattgaga tactatcttg aatcctctca
     4021 taacataaat caccacgaga agtactaaaa taagtagcac gattgttcct gcgttcataa
     4081 ttttattttt ttacggttaa tataatacta ttccttccaa caatttttac cttttcacct
     4141 ttttcaataa gctcatcatc caaggttata gccttccagt cgtctccatc aactacaact
     4201 ctaccggtac ctttagaagt ttcaatagtt tcagaaacta ttgcgattcg tcccatcaaa
     4261 gcatctgcat tagtggctat gtcctttttc ctatttaaaa atttaagggc aaatggtctt
     4321 actaaaacaa agccaagaac tgtgccaatt attaatgaga ggacttgcca attcaagtct
     4381 aatccgcaca aagcggcgat tacggcaaaa acacaaccta ttgcaaaaca aaacacagaa
     4441 aatgttgcag tgaatatttc taaaatcgta agtagaatag cggcaatgag ccaatagtga
     4501 tatggttcca tcctctagtt tattaatttt atgtacaaat atacaattct ttttattatc
     4561 tgcaagaaat aaaaaaataa acattatttt tgtagaattg gattcaaaaa aacttaacta
     4621 taaaacttgt aagcctatag aataaacctt accacaactc caaaccgtac aaacggtacc
     4681 ctaaaaaact cacctgaaat atatcattat gaaaaatacc attcaagtcg tattaatatt
     4741 ttttattgcc atatttatga catcattcga ttttccagca cactataatt ccttcgataa
     4801 ctatcctata tacaatgaca atgatttgga agttacttat accccatcaa aaaccacttt
     4861 caaaatttgg tcgcctgata ttgatagtgc taaagtaaga ctatataaaa caggcattga
     4921 agacgaccca atagaaacta tgttgatgat taaaaaggaa gatggtatat gggaagtaag
     4981 tgcagatgga gatctaaaag gactctttta tacatttcaa gttcaatttg aaaatgaatg
     5041 gttaagagaa agccccggat catacgcaaa agctgttggt gtaaatggat tcagaggtgc
     5101 tattgtcgat atgcaagaaa ccaatcctga aggatgggaa aacgataaac gtcctgctca
     5161 aaaaacattc aacgacatta tgctctacga ggtacatgtt cgagattttt caatggctgc
     5221 aaattcagga atgaagaaca aaggtaagtt tttggcattt acagaagaag gaaccgttaa
     5281 ttcgttcggt caaaaaaccg gtattgacca tttaaaagaa ttgggcataa cacatataca
     5341 tttattgcct tcttttgact atgcatcaat aaacgaaaca aagctaaaag aaaataaata
     5401 caattggggt tacgatcctt caaattttaa tgtccctgag ggcagctatt caacaaaccc
     5461 atacgatcct atttgccgca tcaaagaatt caaacaaatg gtgcaatcat tacacaaaaa
     5521 tggcatccgc gttataatgg acgttgttta caaccatacc ttcaagtgcg aaatatcaaa
     5581 cttcaacatg attacgccgg gttattacta tcgccataat gcagacggaa gtttttcaaa
     5641 tgcatcaggc tgtggtaacg aaactgcttc agaacgtgct atggttcgta aatacattat
     5701 agaatctgta aaatattggg caaccgaata tcatgttgac ggatttagat ttgatttaat
     5761 gggtatacat gatattgaaa caatgaattt aacacgggct gcgttaaacg aaattgattc
     5821 tactattttg atatacggag aaggttggac tgcaggtgat tcacctctcc catacgaaca
     5881 acgtgcagta aaacagaatg ccaatcagat ggacggaatt gctgttttta gtgacgattt
     5941 acgtgatgcc cttaagggta gttgggcaaa tgcaagagaa ccgggatttg cttgtggtaa
     6001 acaaggttac gatgaaacaa ttaaatttgg cgtaatcgga gcaactcaac acgatcaaat
     6061 tcattacgga ctgattaatt actcaaacgc gccttttgca aatcagcctt cgcaagtaat
     6121 taattatgta tcttgccatg atgatttgtg cctaaacgac aaattatcat actcagcacc
     6181 ggaaggagct actccgcaag aacttaagaa gttcaataag tttgctcaat ctattgtatt
     6241 tacagcacaa ggaatacctt tcatttatgc cggtgaagaa cttatgagaa ataaaaaagg
     6301 aatacataat tcgtatcaat cacctgacag tataaatcaa attgattggg atttcaaaaa
     6361 gactaacgaa tctatttatc aatattataa gagtttgatt ttgcttcgca agaatcaccc
     6421 tgccttcaga atgtccacta caaaaatgat tcaagacaac ctacattttc ttgatccggg
     6481 tttggcgtgc gtagttgcat acactatcga caacaacgct aatggtgata gttggaatag
     6541 aatattagtt gtacacaatg gcaatagaca cgaaatcagt ttagacatac caaaagaaaa
     6601 ttggtgggta ataagtaacg gagagatcat caaccaaaat ggtatattta aatttaataa
     6661 agaatatttg acagtaccgg catcatcaac aatgatttta tgcaaaccct aacaagttct
     6721 gacatcgcaa aatatataga agaatatgca cctctttcgt tacaagaaga gtgggataat
     6781 tgtggatggc aactaactaa tggtgatgca atcgtaagtg gagtaatgct ttgcgtagac
     6841 ataaccgaaa gtgtaataca tgaagcaatt gagaaagatt gcaattttat actttcacat
     6901 catcctttaa tatttgacgg attaaaaaaa attacagatg aaccgattag ccgttgtgta
     6961 agaacagcaa tcgaacacaa tattgcattg tattcagccc acacaaacat cgataaagta
     7021 tttaacggcg taagtgctgg aatgtgtaaa aaactgaagc taaataactg caaaatattg
     7081 gaatctgatt gtacaaatag cggttttggc atgataggaa cattgaataa gcctattagt
     7141 tgtacggatt tcttaaaaca attgcaatat acattcaatc ccatatgcct tagacattca
     7201 caaattaccg atatggctat taaaaccgta gctgtttgcg gaggtagtgg atctttcttg
     7261 atttcgagag caaaagaact ctgcgcagat atatttgtta cagcagattt aaaatatcat
     7321 gattttttta aagctgatga aagatttatt ttagctgata tcggacattt tgaatcagaa
     7381 caatacacaa aagagatttt ttttgaaata ctttcaaaaa aaatgcctac ctttgcaatc
     7441 ctaaattcag aaaaggagca taatccaata tattgtctat aaaatccagc attatggcag
     7501 aaaaaactca ggacgcaaaa gaaatttcag tagaacagaa actgaaagca ttgtatgatt
     7561 tacaaacaat cgtgtcagac atagataaaa tcaaaatttt acgtggtgaa ctacctggcg
     7621 aagtacaaga tttagaagat gaaatcattg gtttacaaac tcgtctaaaa aacattgaga
     7681 cagaaataac caatatcaat gcagaaattg caaaacgcaa tcagaatatt atcgattcaa
     7741 atgcattgat tgcaagatat aaagagcaaa tggataatgt tcgcaataac cgtgaatatg
     7801 accatttgac caaagaaatg gaattccagt cattggaaat tgaattagca caaaagaata
     7861 ttcgcgatta tagtgttgct attgaacgca aacacgaaga attcgaaaaa tctaaatatt
     7921 tattggaaga caaaacaatt gtcctcaacg aaaagaaaga agaattagac gaaatcattg
     7981 ttgaaacaaa acaagaagaa gaacgcttac gcgaagatgc taagaaaatt gagacaataa
     8041 tagaatcccg tttattacag gcatttaaac gtattcgtaa aaatgcacat aatggattag
     8101 cagtcgtata tgttcaacgt gatgcttgtg gtggttgctt taataaaatt ccggcacaac
     8161 gtcaattaga cattcgccta cgcaagaaaa tcattgtttg cgaacactgc ggacgtatct
     8221 tgattgaccc tgaattagca ggagttgttg aagaaagcaa gtaataacaa aattatattt
     8281 aataaaaaag tctcgaagta attcgagact tttttatgct ttattgcgaa aatgagtaat
     8341 ttttattacg tcggcaatta tctcagcagt tttttcaata ccgcaaacag aagaattaat
     8401 acacaaatcg taggttgatg ctacacccca ttctgtgttc gaataataat tataatatga
     8461 tgcacgagat ttatctgctt tttttaccaa actctcaata ttttctcctt ttatatcata
     8521 ctccttaaga ttcaatactc gttttacacg tgtttcaata tcggcagtta tgaacacaga
     8581 aaacaatgcc gggtcatccc ttaatacata gttagcacat ctaccaacta ttacgcaaga
     8641 gttttgagca actcgtttta tagtctcaga ttgaaactta aacaaagatt ctcctgataa
     8701 taaattatat tgtcctgacg gcacaaacat tgatcctaaa gaccaatcca aattcaaagc
     8761 cgacaacaag cctcctgatt gacgttcatc agcactttta aagaactcat ctttaatacc
     8821 gctgtcttca gacgcacatt taataagttc cttatcgtaa taaggaatat taaaatactc
     8881 ggctagcatt ctgccaataa cacgtccccc gctaccaaat tgcctgccta ttgaaataat
     8941 tattttattt tcagccatag atgataattt agttattttg atattcagct ttaaactgct
     9001 taatttgatt cattaataat attgaaacaa caattgtaga tacaaaatcc gccatcggca
     9061 tactccacca aacaccttca attccgaaaa atcttggcaa aacaagcaga aatggtatca
     9121 aaaaaagcat ctgtctattt aaagacaaaa aaattgcctt tttagcaagc cctactgatt
     9181 ggaaaaaatt agtagcaacc atctgatatc ctactacagg aaatataaat gtaataatat
     9241 gtaaggcatg cttacttatc tcaattaatt gtgggtctct agtaaataat gaaactacaa
     9301 atgttggaaa gcactgacaa att
//
LOCUS       DAOPID010000034         9318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_9227_length_9318_cov_4.985750, whole genome shotgun sequence.
ACCESSION   DAOPID010000034 DAOPID010000000
VERSION     DAOPID010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 9318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9318
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9227_length_9318_cov_4.985750"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PK641_02245"
     CDS             <1..213
                     /locus_tag="PK641_02245"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPG55210.1"
                     /translation="IYKIYFRHILPTIGGIISGDKAAYQYLNKSVELFPKEQEILSLL
                     RDCGFAAARHKSFTQGLCRMYVANKG"
     gene            complement(210..1115)
                     /gene="menA"
                     /locus_tag="PK641_02250"
     CDS             complement(210..1115)
                     /gene="menA"
                     /locus_tag="PK641_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HPG55211.1"
                     /translation="MNKIPLYFQTFRVRTLPLSMSSIVLGTMMAAVHGFFNVGVCILT
                     VLTTLFLQILCNIANELGDAQKGTDTSDRQGPAYVLTSGALTEKDFYHLIYTFVALSC
                     ISGLILLWLASANISLIYIWMMLALGAFAIIAAIFYTMGKNPYGYKGFGDISVFMFFG
                     LLGTLGSYFLQVGNVELEMILPATACGLLSVGVLNVNNIRDMETDSKNRITIPILLGE
                     KKAKIYHTILIVGGQLCFVAYNIIKYAASLKAYMFLFMLPIFIFHIISVWKLSGKALD
                     KQLPLLVISTMLECLAFGGSWMLLN"
     gene            1368..1802
                     /locus_tag="PK641_02255"
     CDS             1368..1802
                     /locus_tag="PK641_02255"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HPG55212.1"
                     /translation="MIKQEYKHSELTSKIIGCAMTVHKTLGNGFQEVIYQRALEIEMM
                     KEEIAFNREFEMPIFYREQQIGTRRVDFLVEGIVSVELKAMTKLEDVHFAQAINYLEA
                     YNLEIGLLINFGETSLNFKRLTNKKFQPAITQSQKSNKSNKS"
     gene            2010..2091
                     /locus_tag="PK641_02260"
     tRNA            2010..2091
                     /locus_tag="PK641_02260"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2044..2046,aa:Leu,seq:tag)
     gene            2142..3500
                     /gene="tig"
                     /locus_tag="PK641_02265"
     CDS             2142..3500
                     /gene="tig"
                     /locus_tag="PK641_02265"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPG55213.1"
                     /translation="MNVSQNNLDAVNAKLLVKIEPADYTEKVEKAVKDFRKKANMPGF
                     RPGTVPVGLVKKMYGKSFLAEEVNKLISDALYDYIKEKDLNILGEPIPTENFPVPEFE
                     DGANFEFSFDIAIAPELNVSLSAKNKVKYYNITVGKDLIDKQTAAYRGRFGKYVQTEK
                     VEEKDVVKGELQELNADGSEKVDGVKVENALLSPGYIKSADEKKKVLGKTKDSSFVFN
                     PNKAFEGNDAELASLLQISKDAVKTFTADCKFTIKEITRYAEAEMNQEFFDACFGKDV
                     VKSEDEFTAKIKEGLAEQFVEDSDIKFIIDMRDLVLGQLKDVQFADAFLKRWLKANNE
                     KMTAEQIEEEYPKMIEDLKWQLVKDKMAKEAGVKVEKADIEEVAKKAAKAQFAQYGMI
                     GVPDDILDGYVQDMLKNKDGAKNAAERAAEHKIVELLKSKVSLANTDITFDEFSKFFE
                     KK"
     gene            3682..4353
                     /gene="clpP"
                     /locus_tag="PK641_02270"
     CDS             3682..4353
                     /gene="clpP"
                     /locus_tag="PK641_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HPG55214.1"
                     /translation="MNTEFQKYATKHLGLNSIALEKYMDITSNYISPTIIEERQLNVA
                     QMDVFSRLMMDRIIFLGTQVDDYTANVIQAQLLYLDSADPGKDISIYLNTPGGSVYAG
                     LGIYDTMQFISSDVATICTGMAASMGAVLMTAGAKGKRSALKHARIMIHQPMGGAQGQ
                     ASDIEITAREIQKLKKELYTIIAEHSGNTYERIEQDSDRDYWMTAEEAKAYGMIDEVL
                     IKQTK"
     gene            4353..5576
                     /gene="clpX"
                     /locus_tag="PK641_02275"
     CDS             4353..5576
                     /gene="clpX"
                     /locus_tag="PK641_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HPG55215.1"
                     /translation="MAKKEEGRCSFCGRSESEVALLIHGATGAICSDCVERAEEIVNE
                     TIQKKSKFDLSLSNLPKPMQIKQFLDQYVIGQDEAKKNLCVAVYNHYKRLLQHKTEDE
                     VEIEKSNIILVGSTGTGKTLLAKTIAKKLHVPFTIVDATVLTEAGYVGEDIESILTRL
                     LQVADYDVSLAERGIVFIDEIDKIARKGDNPSITRDVSGEGVQQGLLKLLEGSVVNVP
                     PQGGRKHPDQKMIQVNTQNILFICGGAFDGIEKKIGARLNTRMVGYSASKDMHKVDRD
                     NLLQYLAPQDLKAFGLIPEIIGRLPIITHLDPLDRDALRRILIEPKNSIIKQYNKLFA
                     MDGVKLTFDEDALDYIVDKAIEYKLGARGLRSLSETVMMDYMYEIPSEGVKELLITKE
                     IANNRINRMEKKMLI"
     gene            5632..7812
                     /gene="recQ"
                     /locus_tag="PK641_02280"
     CDS             5632..7812
                     /gene="recQ"
                     /locus_tag="PK641_02280"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HPG55216.1"
                     /translation="MSEHSYLTKPLKEHFGFDSFKGNQEAIIENLLAGNDSFVLMPTG
                     GGKSLCYQLPSLLMDGVAIVISPLIALMKNQVDAMRSFSEEDRIAHFINSSLTRAAID
                     EVKHDVLAGKTKLLYVAPESLTKEENISFLQGVRISFYAVDEAHCISEWGHDFRPEYR
                     RIRPMISEIGNAPVIALTATATPKVQHDIQKNLGMNNATVFKSSFNRPNLYYEIRPKT
                     QNIDKDIIRFIKSQEDKSGIIYCLSRKKVEDLAETLVVNGISALPYHAGMDSAVRTEN
                     QDSFLMEKVQVIVATIAFGMGIDKPDVRFVIHYDIPKSLEGYYQETGRAGRDGGEGLC
                     IAFYSYKDLQKLEKFMQGKPISEQEIGKQLLNETAAYAESSVCRRKLLLHYFGEEYTQ
                     DNCCNCDNCLNPKKQMEAQDLLCTVLDTIIAVKEKFKAEHIVDVIVGKETSEVLDYKH
                     EDLEVFGTGQHEEPGVWQAVIRQAMIAGYIDKEIENYGVLKVTKEGKHFLDNPSSFKI
                     TKDNEFDEEEEDTPVRRGGSCAADETLFVMLKDLRKKMSKQLGVPPFVIFQDPSLESM
                     ATTYPVSMEELQNIPGVGAGKAKRYGEDFLKLIQQHVKENEIERPEDLRVRTIPNKSK
                     MKVFIIQAVDRKISLEDIAVSKGLDFSELLDEIEAIIYSGTKINIDYFIDDAMDPDKA
                     EEIFEYFRESQTDDIDTAMTELGGDYTEEEIRLIRIKFISEIGN"
     gene            complement(8018..8791)
                     /gene="rsmA"
                     /locus_tag="PK641_02285"
     CDS             complement(8018..8791)
                     /gene="rsmA"
                     /locus_tag="PK641_02285"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPG55217.1"
                     /translation="MTYVKPKKHLGQHFLKDMNISKRIAETIKTDKYVNVLEIGPGTG
                     VLTQFLLQDSNVDLQVIELDSESVDYLKENFPALEGRIIFDDFLKYDLSKLFNGESFV
                     LIGNYPYNISSQIFFKALDNKERIPFISGMLQKEVAERLASKPGKKAYGILSVLLQAY
                     YDIEYLFTVPPGVFNPPPKVDSAVICMRRNNVQKLDCDEVLFKTVIKTSFNQRRKTLR
                     NSLKSIFPPNFVADNEIFNQRPEQLSVAEFVQLTKMLDL"
     gene            8841..>9318
                     /locus_tag="PK641_02290"
     CDS             8841..>9318
                     /locus_tag="PK641_02290"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HPG55218.1"
                     /translation="MINRCGKFIHSVLINIFDFFFPVFQRWMPKEVYHYLACGTINTL
                     SDWVLYFLIYNYVVHKQIVDLDFIAFSPHIASLIIVTPITFFIGFCLSKYVTFTQSNI
                     QTNQQIKRYAYILAANFLISYFGLKLCCEVLNIWATPAKMITTVFTTLFSYFTQKYY"
BASE COUNT         3239 a         1576 c         1702 g         2801 t
ORIGIN      
        1 atatataaaa tctattttag acatatacta cctactatag gcggtataat atccggagat
       61 aaagctgcat atcaatactt aaacaaatct gttgagttat ttcctaaaga acaagaaata
      121 ctctctctac ttagagattg tgggtttgct gctgctaggc acaagtcatt tacacagggg
      181 ctctgtcgaa tgtatgtagc aaataagggt taatttaata acatccacga cccgccaaaa
      241 gccaaacatt ctaacattgt agatattacc agtagtggta attgcttgtc taatgctttt
      301 cctgataatt tccaaacaga aataatgtga aagataaata ttggaagcat aaataagaac
      361 atatatgctt ttaatgaggc tgcgtattta ataatgttat aagcgacaaa acacaactga
      421 ccacccacta ttaatattgt atgatagatc tttgcttttt tttctcctag taagataggt
      481 attgtgattc tattcttact gtcagtttcc atatcacgta tgttatttac gtttaatacg
      541 cctacagata acaaaccaca agctgtagcc ggcaatatca tctctaattc tacattacct
      601 acctgcaaaa aatacgagcc taatgtacct aacaaaccaa agaacataaa caccgatata
      661 tctccaaatc ctttatatcc gtatggattc tttcccatag tataaaagat tgcagctatt
      721 attgcaaatg ctcctaacgc aagcatcatc caaatatata ttaatgaaat attagccgaa
      781 gccagccata ataaaattag gccggatatg cacgataagg ctacaaacgt atatatcaaa
      841 tgatagaaat ctttttcggt caatgcacct gatgttaaca catatgcagg tccttgtcta
      901 tcggatgtat ccgtaccttt ttgtgcatcg cccaattcat tggcaatatt gcatagaatt
      961 tgtaaaaaca aggttgtcag gactgttaaa atgcaaactc ctacattaaa gaaaccatgt
     1021 acagcagcca tcatagttcc tagaactatg ctcgacattg ataatggtaa ggttcttaca
     1081 cgaaatgttt gaaagtataa aggtatctta ttcatattat tctgttttaa ggcacaaaag
     1141 tacttcaaaa aatctaataa tcacagtcaa aatgatatat gaaaaaaaaa cttattaaga
     1201 tatttcgcac tgattttttg actttgttat tgtatctatt tacctttgcg aaacttttga
     1261 ctatgactat tctgaaaatt ggctcggtga gcctcccgct attgcggttc tttgcccctt
     1321 gcattcaaaa agtcagaact atgattttta tgattaactg attaactatg ataaaacagg
     1381 aatataaaca ctcagaactt acttctaaaa ttattggctg tgctatgaca gttcataaaa
     1441 ccttgggcaa tggctttcaa gaagtaatat atcaacgtgc tttagaaatt gagatgatga
     1501 aggaagagat cgcttttaac cgtgaatttg aaatgccaat attttataga gaacaacaaa
     1561 ttggtactcg tagagttgat tttttagtag aaggcattgt ttcagttgaa ttaaaagcaa
     1621 tgaccaaact tgaagacgtt cactttgcac aggcaattaa ttatttagaa gcatacaatt
     1681 tagaaattgg tttacttatc aattttggtg aaacaagttt gaatttcaaa cgacttacaa
     1741 ataagaaatt ccaaccagca atcacacaat cacaaaaatc aaacaaatca aacaaatcat
     1801 agttcagaca tcctcccccg ctagcgagca ggttttaggt atgatgatac cgatgtaata
     1861 aataaactct ttgacaaaaa tagtggtctt ttagtttcgt ataacgattt taaaaagcaa
     1921 cttactgcag ctttcaaaac acattttaat ttttaatcaa ctattttttt gtcagttatc
     1981 agctttttac taactttgca cccacttttg cggctgtggc gtaattggta gccgcgccag
     2041 acttaggatc tggtgccgag aggcgtgtgg gttcgagtcc cgtcagccgc acaaattaaa
     2101 aaaggggagt gtcctagact ccccgttaat tttaaaacaa catgaacgtt tcgcaaaaca
     2161 atcttgatgc ggtaaacgca aaattgctgg taaaaattga gccggctgac tacactgaaa
     2221 aagtagaaaa agcagtaaaa gattttcgta aaaaagctaa tatgccggga ttccgcccag
     2281 gcaccgtacc tgttggtctt gttaaaaaga tgtacggaaa gtctttcctt gctgaagaag
     2341 taaacaaact tatttctgat gctctttacg attatatcaa ggaaaaagat cttaatattc
     2401 ttggagaacc aattccaact gaaaatttcc ctgtacccga atttgaagat ggtgctaatt
     2461 ttgaattttc ttttgacatt gcaattgcac ctgaattgaa tgtttcttta agtgcaaaaa
     2521 acaaagtaaa atactacaat attacagtag gtaaagatct tatcgataaa cagacagctg
     2581 cataccgcgg acgctttggc aaatatgttc aaactgaaaa agttgaagaa aaagatgttg
     2641 ttaaaggcga attgcaagaa cttaatgcag atggttctga gaaagttgac ggagtaaaag
     2701 ttgagaacgc tttattgagc ccgggatata ttaaaagtgc agacgaaaag aagaaagttt
     2761 taggaaagac taaagattct tctttcgtat tcaatcctaa caaagcattt gaaggtaatg
     2821 acgctgaact agcatcatta ttgcaaataa gcaaggatgc tgtaaaaaca ttcaccgcag
     2881 attgcaaatt tacaatcaaa gaaattacac gctacgcaga agctgaaatg aatcaagaat
     2941 tttttgatgc ttgcttcggt aaagatgtag ttaagtctga ggatgaattt acagcaaaaa
     3001 tcaaagaagg cttagctgaa caatttgtag aagatagcga tataaaattt attattgata
     3061 tgcgtgattt agttcttggt caactaaaag atgtacaatt cgctgatgca ttcttgaaac
     3121 gttggttaaa agctaacaac gaaaaaatga ctgcagaaca aatcgaggaa gaatatccta
     3181 agatgattga agatttgaaa tggcagttgg ttaaagataa aatggcaaaa gaagcaggtg
     3241 ttaaggttga aaaagctgac atcgaagaag ttgccaagaa agcagctaaa gctcaatttg
     3301 cacaatatgg catgattggc gtacctgacg atatcttaga tggttatgtg caagacatgt
     3361 tgaaaaacaa agatggtgct aaaaatgcag ctgaacgcgc tgctgaacac aaaatagtag
     3421 aattgttgaa atctaaggtt agtttggcaa atactgacat cacatttgac gaattcagca
     3481 agttctttga gaagaaataa gttgtttttt cacatatttg taacaaaaag gaatcatttg
     3541 tgattccttt tttgttttat atactttaag atttattttt ttgtgaatta gtatttgctc
     3601 ttgttttaat aaaataaaat tatatatctt tgcttccctt tctttataaa acagattatt
     3661 tatcataatt atatatacat tatgaataca gaatttcaaa agtacgcaac caagcatctt
     3721 ggtttgaatt ccattgcact tgaaaagtac atggacatta ctagtaatta tatttctccg
     3781 acaattatag aagagaggca gcttaatgtt gcacaaatgg atgtgttttc gcgtctaatg
     3841 atggaccgca taatattttt aggtacacaa gttgacgatt acactgccaa tgtaattcaa
     3901 gcacaattat tgtatttgga ttctgcagat cccggcaaag acatctctat atacctaaat
     3961 actcccggag gatctgtata tgcaggttta ggcatctatg atacaatgca gtttatatca
     4021 agtgatgttg caacaatctg taccggtatg gcagcatcaa tgggagctgt acttatgaca
     4081 gccggagcta aaggcaagcg ttctgcttta aaacacgccc gtataatgat tcatcaacct
     4141 atgggtggag cacaaggcca agcctctgat attgaaatta ctgctcgcga aatacaaaaa
     4201 ctcaaaaaag aattatacac cattattgct gaacattcag gtaatactta cgaacgcata
     4261 gaacaagact cagatcgtga ttattggatg accgcagaag aagccaaagc ctacggtatg
     4321 attgatgagg tgttaattaa acaaacaaaa taatggcaaa aaaagaagag ggcagatgca
     4381 gtttttgtgg cagatctgaa agcgaagtgg ccttgttgat acatggtgca acaggagcta
     4441 tatgtagcga ttgtgtagaa agagccgaag aaattgtaaa cgaaactatt caaaaaaagt
     4501 caaaatttga tttgtccttg tctaatctgc ctaagccgat gcagattaaa caatttttag
     4561 accaatatgt aattggccaa gatgaagcaa agaaaaattt atgtgttgct gtttataacc
     4621 actataaacg tctattacaa cataaaaccg aagacgaggt tgaaatagag aaatcaaaca
     4681 taatacttgt tggatcaaca ggaacaggta aaactctatt agcaaaaacc attgcaaaaa
     4741 aattacatgt cccgtttaca attgttgatg ccacggtgct aactgaggca ggatacgttg
     4801 gtgaagatat tgagagcatc ttaactcgcc tattacaagt tgcagattac gatgtttcat
     4861 tagctgagcg aggcatcgtt tttattgacg agattgataa gattgcacgt aaaggagata
     4921 atccgtctat tacgagggat gtaagtggag aaggtgttca gcaaggttta cttaaattat
     4981 tggaaggttc tgttgtgaat gtcccacctc aaggaggtcg taagcatcct gaccaaaaaa
     5041 tgattcaggt caacactcaa aacattttat ttatttgcgg cggtgcattt gatggcattg
     5101 aaaagaaaat cggcgccaga ttaaatactc gaatggtagg atattctgcc tcaaaggata
     5161 tgcataaagt tgatagagat aatttgctgc aatatttagc tccacaagat ttaaaagcat
     5221 tcggattaat acctgagatt ataggtagat tacctataat tacacatttg gacccattag
     5281 atagagacgc acttcgccgt atattgattg aacctaagaa ctccattatc aaacaatata
     5341 acaaattgtt tgcaatggat ggcgtaaaat taacattcga tgaagatgct cttgattata
     5401 ttgtagataa agccatagaa tataaattag gcgcacgtgg tctacgctcc ttaagcgaaa
     5461 ctgttatgat ggattatatg tacgaaatcc catcagaagg cgttaaagaa ttactaatta
     5521 caaaagaaat agctaataat aggattaata gaatggagaa aaaaatgctg atttaaaaat
     5581 atgtgttaaa atcttgcatt acaaattcta tttattatat ttgctctgat tatgtcagaa
     5641 cactcatatt taacaaaacc attaaaagag cacttcggtt ttgattcctt taaagggaac
     5701 caagaagcaa ttatcgaaaa tttacttgcg ggcaatgatt catttgtact tatgccaaca
     5761 ggtggaggga agtctttgtg ctatcaactg ccatcattat tgatggacgg ggttgcaatt
     5821 gttatttctc ctcttatcgc attaatgaaa aatcaggttg acgctatgcg ctcatttagt
     5881 gaggaagaca gaatcgccca ttttattaat tcttctctta cacgtgcagc tattgacgaa
     5941 gttaaacacg atgttcttgc aggcaaaaca aaattacttt atgtggctcc tgagtcactt
     6001 acaaaagaag aaaatataag ttttttacaa ggcgtacgta tctcattcta cgcagtagat
     6061 gaggcgcatt gtatctcaga atggggacac gattttagac cggaatatcg ccgcatcaga
     6121 cctatgatat ctgagattgg gaatgcacct gtaatagccc ttacagcaac tgctacacca
     6181 aaggtgcagc acgatattca aaaaaacctt ggtatgaata atgccaccgt ttttaaatca
     6241 tcatttaaca gacctaacct atattacgaa atacgtccta agacacagaa tattgataag
     6301 gatattatca gattcataaa aagtcaagaa gataaatccg gcatcattta ctgtttaagt
     6361 cgcaaaaagg ttgaagattt agccgaaaca cttgtagtaa atggaatcag tgcgctgcca
     6421 tatcatgcag gtatggattc tgccgtaagg acagaaaatc aggatagttt cttaatggag
     6481 aaagtgcagg taattgttgc aaccatagcc tttggtatgg gtattgacaa gcctgatgta
     6541 cgctttgtta tccactatga cattccaaaa agtttggaag gatactatca agagacagga
     6601 cgcgcaggac gtgatggagg agaaggttta tgtattgcat tttattctta taaagattta
     6661 cagaaattag aaaaattcat gcagggcaaa cctatttcag aacaagaaat aggtaagcaa
     6721 ttgttgaacg aaacggctgc ttacgcagaa tcatcggttt gtagaaggaa attattacta
     6781 cattattttg gagaagaata tacccaagac aactgctgta actgcgacaa ctgcttgaat
     6841 ccaaaaaaac aaatggaagc acaggactta ttgtgtaccg tcttagatac aatcatagcg
     6901 gtaaaagaaa aatttaaagc agagcatatt gttgacgtta tagttggaaa agaaacgtca
     6961 gaagtgttag actataagca cgaagattta gaggtgttcg gcacaggcca acacgaagaa
     7021 cctggcgtat ggcaagcagt aattcgtcaa gcaatgattg cgggttacat tgataaagaa
     7081 atagaaaact acggagtgct aaaggttaca aaagaaggta agcatttcct tgataatcca
     7141 agttcattca aaattacaaa agataatgaa tttgatgagg aagaagaaga tacaccggta
     7201 aggcgaggcg gatcttgtgc agcagatgaa acattatttg taatgctaaa agatttgcgc
     7261 aaaaaaatgt ctaaacaatt aggtgttcct ccttttgtaa tattccaaga tccatcactc
     7321 gaatcaatgg ccacgaccta ccctgtcagc atggaagagt tgcaaaacat cccgggcgtt
     7381 ggtgcaggta aggctaaacg ttatggcgag gatttcttaa agcttattca gcaacacgta
     7441 aaagagaatg aaatagaacg tcctgaagat ttgcgcgtac gtactatacc taacaaatcg
     7501 aaaatgaagg tatttattat acaagctgtt gataggaaaa tcagtttgga agatattgca
     7561 gtatctaaag gacttgattt ttctgaatta cttgatgaaa ttgaagccat catatactca
     7621 ggcacaaaaa tcaatatcga ctactttatt gatgatgcta tggatcctga taaggctgag
     7681 gaaatctttg aatatttccg cgaatctcaa actgatgata ttgatactgc catgactgaa
     7741 ttaggcggcg actatacaga agaagaaatt agattaatac gtatcaagtt catttctgaa
     7801 atagggaact aaaattttca ataatatttt taaaccaatt caaaataagc gttctgcttt
     7861 ttgaattggt tttttatgct gattagacaa gatcttaaca tttcccaaag tcttacagaa
     7921 gttgattttt tcaaacaacc ttttagcatc tgtaacaact ttattacata acattgacca
     7981 ttgtaattag atggagcaca gcatattgcg tacaaaacta caagtctagc atctttgtaa
     8041 gctgaacaaa ttcagccaca ctcaattgct cgggacgctg attaaaaatt tcgttgtctg
     8101 ctacaaaatt aggaggaaat atcgacttaa gagagtttcg caatgttttg cgcctttgat
     8161 taaatgaggt tttaataaca gttttaaata agacctcatc acaatcaagt ttctgcacat
     8221 tatttcgcct catacagata actgcagaat cgacctttgg tggtggatta aaaacaccgg
     8281 gaggaacagt aaataaatat tctatatcat aataagcttg taatagaaca ctcaatattc
     8341 cgtatgcttt tttacccggc tttgatgcta aacgctctgc cacttctttt tgcaacatcc
     8401 cactgataaa aggtatacgc tccttattat ctagggcttt aaagaaaatt tgactcgaaa
     8461 tattatatgg gtaattgcca attaacacaa atgactcgcc attaaaaagt ttagacaaat
     8521 cgtattttaa gaaatcatca aaaataatgc gaccctctaa agcgggaaaa ttctctttta
     8581 aataatccac cgattcgctg tccaattcta ttacttgtaa atcaacatta gaatcttgca
     8641 acagaaattg agtaaggact cccgttcccg gacctatttc taacacatta acatatttat
     8701 ccgtctttat tgtctctgca atgcgctttg aaatattcat atccttgagg aaatgttgtc
     8761 ctaagtgctt tttcggtttt acgtatgtca tttgtataat ttgtttatct ttgcaaagat
     8821 aaataaaacc atcgactgct atgataaacc gctgcggtaa attcatacat tccgtcttaa
     8881 taaacatttt cgacttcttc ttccctgttt ttcagagatg gatgccaaaa gaagtatacc
     8941 attatttggc atgcgggaca attaatacct tatcagattg ggttttatat ttcttgattt
     9001 acaattatgt agttcataaa caaattgttg atttagattt tattgcattt tctcctcata
     9061 tcgcgagctt aattattgta accccaataa catttttcat aggcttttgc ctatcaaaat
     9121 atgttacatt tactcagtca aacattcaga caaaccaaca aataaaaaga tacgcttata
     9181 ttttggccgc taatttctta atcagttatt ttggactaaa actatgctgt gaagttctta
     9241 atatttgggc aacccctgca aagatgataa ccacggtttt cactacccta ttcagctatt
     9301 tcacgcaaaa gtattatt
//
LOCUS       DAOPID010000035         9279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_9284_length_9279_cov_4.015937, whole genome shotgun sequence.
ACCESSION   DAOPID010000035 DAOPID010000000
VERSION     DAOPID010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 9279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9279
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9284_length_9279_cov_4.015937"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2325
                     /gene="sprA"
                     /locus_tag="PK641_02295"
     CDS             <1..>2325
                     /gene="sprA"
                     /locus_tag="PK641_02295"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HPG55219.1"
                     /translation="VAKVNSSSVSNPFKLQDTGLNGLLTEEEFTFATYKDFVDNMTAK
                     LSPDAIARLQADKLSMLNDPAGDNFHHYRGSDYDEAEVSILDRYKHYNGTEGNSPAST
                     AGESYTTASTNNPDIEDINQDNTMTETEKYYEYKISLRPEDMTIGNNYISDVVTSSVS
                     LKNGETASVKWYQFKIPLRDSEKYSAKGGIRSFKSIRYMRMYLTDFEEPVNLRFATME
                     LVKGEWRSYTNPLEETYYNNNGVIEMSSVSIEENSTKTPVNYVLPPGVSREVDPSQQQ
                     VRQENEQSMTMRVLNLQPKDARALYKKLGGYDMRQYGRIQMFVHAEAIAEDNTDLQDN
                     DLKLFVRMGSDYKNNYYEYEVPLEVTPAGMYSNNSTADRNIVWPEDNMIDFPLSLLTD
                     LKLARNEEKNRQGSHVTNLTPYSEVDPNKPNNKITIKGNPSLGEVEVLMIGIRNGSRI
                     VKSGEIWVDELLLTDFDEKGGVAALASLDVALSDFITLSAAGRLETIGFGGIEQRVSE
                     RRQDDYYQYNFNAGIDLGKLFPEKANVLMPIRFSYSSEITNPKYNPLDKDILLSDALR
                     TASSRAMRDSILQIAQSVSTNRSFSIPNIRVGIVSKKGPRPWDPANFTIGGSYSETSS
                     HNPNTVRNIDQYCNAYGTYEFSWTPKPWEPFGKISKLKNSKNWNWIADIGINYAPKRF
                     AFKTNFARNYYEQLVRDFNNESSEVMDPTFQKDFMWNNDFDIAWDITKNIRLTLTTSN
                     QSRISENEGGVNKWLYPDEYEIWKDSVRNSLAHLG"
     assembly_gap    2326..2335
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2336..4200
                     /gene="sprA"
                     /locus_tag="PK641_02300"
     CDS             <2336..4200
                     /gene="sprA"
                     /locus_tag="PK641_02300"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HPG55220.1"
                     /translation="GSPMNYNQRFTAAWSIPFNKIQALNWISGNMKYDGNYEWLYGNE
                     TIANTATSTGTWAVDGKINFETLYNKSNYLKEVNKKFASSRKSSKNKSTDKTKKFEQT
                     VKFSKGDTVLIKHKLNSKSVDVIASLNGKKYPIKYKIVDLNTISFIAKNDAEIDLSVA
                     MPNGDKPANMALDITSRILMLVRNVSFNYSQTDGTILPGFKPSVTCLGLNSYNGTTAP
                     GWDFVFGVQNSDILNRAINNNWLVMEDVIYNPAVMSHSETFKASALLEPFPGFKINLA
                     ADRQYVNNRSVQYNTDNRLTTMAGSLSMSYVAIGTSFASMARGSNYSSDLFSQFLEYR
                     NTVRSRLNNKYSPTSSYGLNTDDVLIPAFLAAYSGRDPNKVGLSAIPSFWSFLPNWQI
                     TFDGLSRIPWIKEHFRSVNLTHAYNCKYQMGSFTADQSFMSVDGDDFGYVTDILSGGL
                     TPSSRYTVGSVSINEQFSPLIGVDINMKNSLSVKFEWRKGRNLGLNLSGNQVVESWND
                     EYVVGLGYKFSDLNFTMKSASKQKKVKNDLTLRADFSLKDTETLVRKIELEESQATAG
                     DMVVAFKFAADYVFSERINFRLYYDLQMRNPVVTTSYPTTISDVGIAVRILLTR"
     gene            complement(4238..5317)
                     /locus_tag="PK641_02305"
     CDS             complement(4238..5317)
                     /locus_tag="PK641_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPG55221.1"
                     /translation="MLKRIDFYIIKKVLGTYFLSILMIISIAIIIDITEKMDNFYNEN
                     LSLNTIIMDYYIHFIPFYINLFSSLFAFIAVLFVTSKLAFNSEIIAMLAGGISFKRIL
                     VPYFISAAIIALFTFYIGSYIIPRGNDVRLDFESRYVDHIAVESTLRNVQFKVNANEI
                     AYMERFDLKGEKGYRFSLDKFEGKKLVSRITATTIKHDSLHTWTMYDFMKRDFVGMRE
                     ILTTGDTLKREIEMTPKEFVVVKNHHERMTNPELREYINSQSQRGRAGLNAYKLEYQK
                     RFAMPFGAIILTLIGVSVASRKTRGGAGFHVLIGFALSVTYILCTLVSSSFTVKGGWN
                     PIFAIWLPNIVYSGIGIWLYRIAPK"
     gene            5419..5856
                     /locus_tag="PK641_02310"
     CDS             5419..5856
                     /locus_tag="PK641_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007215009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HPG55222.1"
                     /translation="MLDKVKEYLQVHDVHPSMQRIVIMNYLFNHRTHPTVDVIYNDIS
                     RSMPTLSKTTVYNTLKVLVNHNAVLQLDIDEKESHYDGYTHVHAHFRCLNCGKIIDLS
                     PKYLNKLVTNEIDDLIITETQLYYKGYCSDCKKNGELPNINNK"
     gene            5866..5973
                     /locus_tag="PK641_02315"
                     /pseudo
     CDS             5866..5973
                     /locus_tag="PK641_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856013.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
     gene            6011..6553
                     /locus_tag="PK641_02320"
     CDS             6011..6553
                     /locus_tag="PK641_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HPG55223.1"
                     /translation="MKFICSVCGYVHEGDAAPEKCPQCGQPASKFTKLDENAKLNFVT
                     EHVIGIAKDCDDEMKKDLNAHFMGEATEVGMYLAMSRQADREGYPEIAEAFKRYAFEE
                     AEHCARFAELLGDVVWDTKTNLDKRMNAESGACADKMRIAKRAKELNLDAIHDTVHEM
                     AKDEARHGRGFEGLYNRYFG"
     gene            complement(6615..7607)
                     /locus_tag="PK641_02325"
     CDS             complement(6615..7607)
                     /locus_tag="PK641_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPG55224.1"
                     /translation="MYTPNPDRYNNKTKYNYCGKSGLMLPRLSLGLWHNFGSFDNFDE
                     AEKMLFLAFDEGITHFDLANNYGHPAGSAESNFGKLFNKHFSSHRDEMTITTKAGFFM
                     WDGPYGDGSSRKYLISSLDQSLKRLGLDYVDIFYSHRYDQNTPIEETVQALTDIVHQG
                     KALYVGISNYPVEAAQKAFWLLNENRTPCIVYQGRYNMLYRDPEISLFDTLRQSKVGF
                     VAYSALAQGILSEKYLNGIPENSRAANQYGYLQKSEITDEIVGKMQKLNAVAQRREQT
                     LAQMSLAWVLRDIHVTSALVGVSTAAQLKSNLDALNNTYFSDEEYIEIDKIIYG"
     gene            complement(7610..8563)
                     /locus_tag="PK641_02330"
     CDS             complement(7610..8563)
                     /locus_tag="PK641_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013065417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HPG55225.1"
                     /translation="MTDESIWYWIGFNVFVLLMLLLDLGVFHKKDEAPKIKESLLWTV
                     FWILLALIFGLGVYVHLGHTKGLEFITGYLLEKSLSIDNLFVFIMLFGIFRIEPKYQH
                     RVLFWGIFGAIILRAIFIFAGVALLNKFAWIMYVFGVFLIYTGIHMLFEKKDEAPHPE
                     NNLIIRGFKKIFPVTHTIENHDFFVKKNAKWHATPLFVALLMIELSDVMFAVDSIPAV
                     LSVSRDTFIVYTSNIFAILGLRSLYFAINGVMEYFYYLKYGLAGILGFIGLKMCLNEI
                     YVDFGWGIEISNVTSLFIIIGFVAVAIIASVIRQKKQTKTI"
     gene            complement(8665..9114)
                     /locus_tag="PK641_02335"
     CDS             complement(8665..9114)
                     /locus_tag="PK641_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HPG55226.1"
                     /translation="MELIELITNDIKKAMLAKDKIRLEALRGIKKELLEAQTAKGSNG
                     VVTEEAGLKILQKMVKQRKESAIIYIEQNRSELAESETAEADIISEYLPKTMSDEELT
                     ACLKEIITQLGINSAKEMGKVMGVASKQLAGKADGRAISEKVKQLLG"
BASE COUNT         3015 a         1484 c         1700 g         3070 t
ORIGIN      
        1 agtagcaaaa gtaaactcct cttcagtaag caatccgttt aaacttcagg ataccggttt
       61 aaacggattg cttactgaag aggagtttac ttttgctact tacaaggatt ttgttgataa
      121 tatgactgca aagttgtcgc ctgatgctat tgccagattg caagcagata aattatcaat
      181 gttaaatgac cccgcaggag ataattttca tcattataga ggaagtgatt atgatgaggc
      241 agaagtaagc attttagatc gttataaaca ttataacgga accgagggta actctccggc
      301 atcaacagca ggcgaatcat atacaacagc ttcaacaaat aatccggata tagaagatat
      361 aaatcaggat aatacgatga ccgaaaccga gaagtattat gaatacaaga tttcgcttcg
      421 tcctgaagat atgaccattg gtaataatta tatatctgat gttgtaactt catccgtaag
      481 tttgaaaaac ggagaaacag catcagtaaa atggtatcag tttaaaattc ctttacgtga
      541 ttctgagaag tattctgcaa aaggcggtat ccgcagcttt aaatcaatta gatatatgcg
      601 aatgtatctt actgattttg aagagcctgt aaatctgcgt tttgcaacaa tggagttggt
      661 taagggcgaa tggagatcat ataccaatcc cttagaagaa acatattaca ataacaatgg
      721 tgttattgaa atgtcatcag taagtattga agaaaattct actaaaactc ctgtaaatta
      781 tgttttacct ccgggggtaa gtcgtgaggt tgatccaagt caacagcagg tacgtcagga
      841 aaatgagcaa tcaatgacaa tgcgtgttct taacttacaa cccaaagacg ctagggcttt
      901 atataaaaaa ttaggtggat acgatatgcg tcagtatggt agaattcaga tgtttgttca
      961 tgctgaggct attgctgaag acaatactga tttacaagat aatgatttga agttatttgt
     1021 tcgtatgggt tctgattaca agaataacta ttacgaatat gaagttcctt tggaagtaac
     1081 tcctgccgga atgtactcaa ataattcaac tgcagataga aacattgtat ggcctgagga
     1141 taatatgatt gatttcccat tgtcattgct tactgatttg aaattagctc gaaatgagga
     1201 aaaaaatcga caaggttcgc atgttactaa tttaacgcct tactctgaag ttgacccgaa
     1261 taagccaaat aacaagataa ccataaaggg taatccaagt ttgggtgagg ttgaagtttt
     1321 gatgattggt attagaaacg gatcaagaat tgtgaagtcg ggcgaaatat gggttgatga
     1381 gttgttactt actgattttg atgaaaaggg tggtgttgca gctttggcaa gtcttgatgt
     1441 ggctttgtcc gactttatca cattaagtgc tgctggtaga ttggaaacga ttggttttgg
     1501 tggaattgaa caaagggtat cagagcgcag acaagacgat tattaccagt ataattttaa
     1561 tgcgggtatc gatttaggta agttattccc tgaaaaggcg aatgttttga tgccgatacg
     1621 tttctcatat tcaagcgaaa ttacaaatcc taagtacaat cctttggata aggatatctt
     1681 attgtcagat gcattaagaa ccgcttcttc tagagctatg cgtgattcta ttttacagat
     1741 tgctcaaagt gtatctacaa ataggagttt ttctatacct aatatacgtg taggaatagt
     1801 tagtaagaaa ggacctagac cttgggatcc tgctaacttt acgataggag gttcgtattc
     1861 cgaaacatct tcacataatc ctaatactgt aagaaatatt gatcagtatt gtaatgcgta
     1921 tggtacttat gagttttctt ggactcctaa accatgggaa ccatttggca aaatttctaa
     1981 attgaagaat tctaagaact ggaattggat agcggatata ggtataaatt atgcacctaa
     2041 gcgttttgca tttaagacca attttgcaag aaattattat gagcaacttg ttcgcgactt
     2101 caataatgag tcgtctgaag ttatggatcc gacattccaa aaagatttta tgtggaataa
     2161 tgatttcgat attgcatggg acataactaa gaacattcgt ttaacattaa ctacttcaaa
     2221 tcaatctcga attagcgaaa atgaaggtgg tgtaaataag tggttatatc ctgacgaata
     2281 tgaaatatgg aaagattccg tacgtaatag tttagctcat ttaggnnnnn nnnnntaggt
     2341 tctccaatga attacaatca acgctttact gcagcatggt ctattccatt taataagata
     2401 caagctttaa attggatatc cggtaatatg aaatacgatg gtaattacga gtggctatat
     2461 ggaaacgaaa ctattgccaa tactgctaca agtactggca cttgggctgt tgacggaaaa
     2521 attaatttcg aaactttata taataaatcg aattatttga aagaggtcaa taagaagttc
     2581 gcttcgtctc gtaaatcatc taaaaataaa tctacagata aaacaaagaa atttgagcag
     2641 actgttaagt ttagcaaagg cgatactgtt ttaattaagc acaaacttaa ttcgaaaagt
     2701 gttgatgtta ttgcatcttt aaatggtaag aaatatccta taaaatacaa gattgtcgat
     2761 ttaaatacaa tatcgttcat tgctaaaaat gatgctgaaa tagatttatc tgtggctatg
     2821 cctaatggtg ataaacctgc aaatatggct ttggatataa catctcgtat tttaatgtta
     2881 gtgcgtaatg tatcatttaa ttattctcaa accgatggta ctatattacc cggatttaag
     2941 ccttctgtta cttgtttagg tttgaatagt tataatggta caacggctcc cggttgggat
     3001 tttgtattcg gtgttcaaaa tagcgatata ttgaatagag ctatcaataa taactggttg
     3061 gttatggagg atgttattta taatccggcg gttatgtcac attccgaaac atttaaggca
     3121 tcagccctat tagagccttt ccccggattt aagattaatc ttgctgcaga caggcagtat
     3181 gtcaataaca gaagtgtaca atataacacg gataatcgtc ttactaccat ggcaggaagt
     3241 ttgtctatga gttatgtagc tataggaaca tcatttgcat caatggctag gggtagtaat
     3301 tattcgtctg atttgtttag tcagttcttg gaatatagaa acacagtaag gtcacgtttg
     3361 aataataagt atagtcctac ttcttcttat ggtctaaata ctgatgatgt tttgatacct
     3421 gctttcttgg cggcttattc aggtcgagat cctaataagg tgggtttaag tgcaattcct
     3481 tcattttggt cgttcttgcc taactggcaa attactttcg atggattgtc acgcatacca
     3541 tggattaagg aacattttag aagtgtcaat ttaactcatg catataattg taagtatcag
     3601 atgggttcgt ttacagcaga ccagtcattt atgtcggttg atggagatga tttcggttat
     3661 gttactgata tacttagcgg tggtttgacc ccatctagcc gctatacggt aggttctgta
     3721 tcaattaacg aacaattcag tcctcttatt ggtgtggata ttaatatgaa gaatagttta
     3781 tctgttaaat tcgagtggag gaaaggacgt aatttaggct tgaatttatc gggtaatcag
     3841 gttgttgaat catggaacga tgagtatgtt gtaggacttg gctataaatt tagtgacctc
     3901 aatttcacaa tgaaatctgc atcaaaacaa aagaaagtta agaatgattt gactttgcgt
     3961 gctgatttct ctttaaagga tactgaaaca ttggtacgca aaattgaatt agaagaatcg
     4021 caagctactg ccggcgacat ggttgtagcg tttaaatttg ctgctgatta tgtgtttagt
     4081 gaacgtataa actttagatt atattacgac ctacagatgc gtaatccggt tgtaacgaca
     4141 tcatatccta caacaatatc tgatgttggt atagctgtta gaattttgct tacaagataa
     4201 aatttaagca gttgggctta aactgttggg tagtagttta ttttggtgcg attctatata
     4261 accaaattcc tatacctgaa taaacaatat tgggaagcca aattgcaaat ataggattcc
     4321 atcccccctt tactgtgaaa gatgaggata caagtgtaca taaaatgtat gtaacactta
     4381 gggcgaagcc tattaaaaca tgaaagcctg cgccacctct ggttttgcgt gatgctactg
     4441 aaactccgat tagcgttagt ataatagcgc cgaacggcat agcgaaacgt ttttgatatt
     4501 caagtttata tgcatttagt ccggctcttc ctctttgact ttgtgaatta atgtattcgc
     4561 gcaattcagg atttgtcatt ctttcgtggt gattctttac aactacaaat tccttaggag
     4621 tcatttctat ttcgcgtttt agagtatctc ctgtagttaa tatttcacgc attccaacaa
     4681 aatcacgttt cataaaatca tacattgtcc atgtgtgtaa cgaatcatgt tttatggtag
     4741 tagcagtaat gcgtgatacc aattttttcc cttcaaattt gtcgagtgag aatctatatc
     4801 ctttttctcc ttttaaatcg aatcgttcca tgtaggcgat ttcatttgca tttaccttaa
     4861 attgaacatt tctaagagta ctttcaacgg ctatatggtc aacgtatcga ctctcgaagt
     4921 cgagtctgac atcattccct cttggaataa tgtatgaacc aatgtaaaaa gtgaataatg
     4981 caattattgc tgctgatata aaataaggaa caagaatacg tttaaaactg atgccacctg
     5041 caagcattgc aattatctca gaattaaatg ccaactttga tgtaacaaaa agtactgcaa
     5101 taaaagcaaa aagtgaactg aatagattta tataaaatgg aataaaatga atgtagtaat
     5161 ccattattat tgtattaagg gatagatttt cgttgtagaa attatccatt ttttcggtaa
     5221 tgtcaataat tatggcaata ctaataatca ttaagatgga caaaaagtat gtccccaaaa
     5281 ctttcttgat aatatagaaa tctattcgtt ttagcatatt gtgctgcaaa agtacagaaa
     5341 attattgata aaaatttggt gaattaaaat aaacaacata catttgcaat gattctagaa
     5401 ttgtaatcat tacaaattat gctggataag gttaaagaat atttgcaagt acatgatgtg
     5461 catccttcaa tgcaacgaat agttattatg aattatttgt ttaatcatag gacacatcct
     5521 actgtagatg tgatatataa tgatatatct aggtctatgc cgactttatc aaagacaaca
     5581 gtatataaca ctcttaaagt tttggttaat cataatgcag tgttgcagtt ggatatagat
     5641 gaaaaggagt cacattatga tggttatact cacgttcatg cgcattttag atgcttgaat
     5701 tgtggaaaaa taattgattt atctccgaaa tatttaaata aacttgtaac aaatgagatt
     5761 gatgatttga taataactga gacacaatta tattataaag gatattgttc agattgtaaa
     5821 aaaaatgggg aactccctaa tattaataat aaataaaaaa aaactattat gaaaactttt
     5881 gtatgcacag tatgcggtta cgtacaccat ggtgatacac cgccggcaat atgtccgaaa
     5941 tgtaagcaac ccggttctaa gtttaaagaa gtaaaagcgt gatgttaata atttttaaaa
     6001 ttaaaatgat atgaaattta tttgttcggt gtgcggttat gtacacgaag gagacgcagc
     6061 tcctgaaaaa tgtcctcaat gtggacaacc tgcaagcaag tttacaaaac ttgatgaaaa
     6121 tgcaaaatta aactttgtta cagagcatgt tataggtatt gcgaaagatt gtgatgatga
     6181 gatgaagaaa gatttaaatg ctcatttcat gggagaagca acagaagtag gcatgtattt
     6241 ggcaatgagt agacaagcag atcgtgaagg ttatcctgag attgcagaag catttaaacg
     6301 ttatgcattt gaagaagcag aacattgtgc gagatttgca gaattgttag gtgatgttgt
     6361 atgggatacc aaaactaatc tagacaagag aatgaatgct gaaagtggag cttgcgctga
     6421 taaaatgcgt attgcgaaac gtgcaaaaga attgaattta gatgcaatac atgatacagt
     6481 tcatgaaatg gcaaaagacg aagctcgtca cggtcgtgga tttgaaggat tgtataatcg
     6541 ctattttgga taaaagatat cctttttaat aggaacggct tccggtaatt tacacagaag
     6601 ccgttttttt attgttaacc atagatgatt ttatctattt ctatatactc ttcatcacta
     6661 aaataagtat tgttaagtgc atctaaatta ctttttagtt gggctgcagt acttacacca
     6721 actagtgcag aagttacgtg tatatctcgt aaaacccatg caagggacat ttgtgctaat
     6781 gtttgttcgc gcctttgtgc tacggcattt agtttttgca tttttcctac aatttcatct
     6841 gttatttcag atttttgcag ataaccatat tggtttgcag ctcttgaatt ttcaggtatc
     6901 ccattaaggt atttttcaga taaaataccc tgtgcaagtg cagaataagc tacaaatccg
     6961 actttacttt gacgtagtgt atcaaataaa cttatttcag gatcgcggta aagcatgttg
     7021 tatcttcctt gataaactat acaaggagtt ctattttcgt ttaatagcca aaatgctttt
     7081 tgagcggctt caacgggata atttgaaata ccaacatata aggctttacc ttgatgtaca
     7141 atatctgtca acgcttgtac agtttcttct ataggggtat tctgatcata tctgtgtgag
     7201 taaaatatat ctacataatc cagacctaat ctttttaaac tttgatccaa actcgaaatt
     7261 aaatacttgc gcgaacttcc gtctccataa gggccatccc acataaagaa acctgccttg
     7321 gtggttattg tcatttcatc tctgtgtgat gaaaaatgtt tattaaataa cttaccaaaa
     7381 ttactttctg cagaacctgc cggatgcccg taattatttg ctaaatcgaa atgagtaata
     7441 ccttcatcaa atgccaaaaa cagcattttt tcagcttcat caaaattatc aaaagatcca
     7501 aaattgtgcc ataaaccaag tgataggcga ggcaacatta aaccgctttt cccacaataa
     7561 ttgtatttag ttttattatt gtatctgtcc ggattaggtg tgtacattgc taaatagttt
     7621 tagtttgttt cttttgtctt ataacagatg ctataattgc aaccgcaaca aatcctatta
     7681 tgatgaataa tgatgtaaca tttgagattt caattcccca accaaaatct acatatattt
     7741 catttaaaca catttttaga ccaataaagc caagaattcc ggctaaacca tatttcagat
     7801 aatagaaata ttccataaca ccgttaattg cgaagtatag tgaacgaagt cctagaattg
     7861 caaaaatatt tgatgtgtaa acaataaaag tatctcttga tacagaaagc actgcaggaa
     7921 tggaatcaac agcaaacata acatcagata attctatcat aagcaatgca acaaataggg
     7981 gagttgcatg ccattttgca tttttcttaa caaaaaaatc gtggttctct attgtatgtg
     8041 taaccggaaa aattttctta aacccacgta taattaagtt attttcggga tgaggtgcct
     8101 catctttttt ctcaaacagc atgtgaatac ctgtgtatat aaggaaaact ccaaacacat
     8161 acattatcca tgcaaatttg ttaagtagtg caacgccggc aaatataaat attgcacgta
     8221 gtattatagc tccaaatata ccccagaata ataccctgtg ttggtatttt ggctctattc
     8281 ggaagatgcc gaacagcatt ataaatacaa ataaattatc tatgctgagt gatttctcta
     8341 ataggtatcc ggttataaat tcaagtcctt ttgtgtggcc gaggtgtaca taaacaccta
     8401 aaccaaaaat aagtgctaat aatatccaaa atactgtcca aagcaaagat tcttttattt
     8461 tgggagcttc gtcttttttg tgaaaaacgc caagatctag caaaagcatc aacaaaacaa
     8521 atacattaaa tccaatccaa taccaaatac tctcgtcagt cataatctat tatttattat
     8581 taatgtgcaa agatagtcat acttcttttg gcccatataa aatttatatg tttttatgtt
     8641 ctgtgttgat aagcacatta tattttagcc taatagttgt tttacctttt ccgaaattgc
     8701 tcgtccgtct gctttaccag ccagttgttt tgatgcaaca cccataactt tgcccatttc
     8761 ttttgctgaa ttaattccta attgtgttat aatttcttta agacaagcag ttagctcttc
     8821 atcactcatt gttttaggta ggtattcaga aataatatca gcttctgctg tttcactttc
     8881 tgcaagttca gatctgtttt gttctatata tattattgca gattcctttc gttgtttaac
     8941 catcttttgc aaaattttta aacctgcttc ttctgttaca actccattac tgccttttgc
     9001 tgtttgtgct tccaataatt ctttttttat acctctcaat gcttccaaac gaatcttgtc
     9061 tttggctaac atagccttct ttatgtcgtt tgtaatgagt tctattagtt ccatatttat
     9121 tatttattgc gtttatatgc cggttcatca aaatcctcac cgtctgccca caaatcaaga
     9181 gaaattactt cttctttctt attataatct tcttcaaatg gatcaatgat gcttggtgga
     9241 gcttcgcttt tttgctcatt ttccccgtag tattcatca
//
LOCUS       DAOPID010000036         9276 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_9291_length_9276_cov_4.688645, whole genome shotgun sequence.
ACCESSION   DAOPID010000036 DAOPID010000000
VERSION     DAOPID010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 9276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9276
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9291_length_9276_cov_4.688645"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="PK641_02340"
     CDS             <1..979
                     /locus_tag="PK641_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3362 domain-containing protein"
                     /protein_id="HPG55227.1"
                     /translation="PLTTEELDSIYDLPYTRLPHPRYKGKTIPAYEMIKYSVSIHRGC
                     FGGCSFCTISAHQGKFISSRSEKSILKEVEKITQDADFKGYVSDLGGPSANMYNIHGK
                     DISICQKCKRPSCLHPHICNNLQTNHERIINLYRQANKINGIKKIFIGSGIRYDLATT
                     DYLEEVVKHHVSGRLKVAPEHTNDAVLNLMRKPSFKFFEAFDKEFERINKESGLRQQL
                     IPYFISSHPGCTTEQMAELASKTKRMHFQLEQVQDFTPTPMTLSTEIYYTGLNPYTLE
                     KVYTANTPALKLEQRSFFFWYKPEERAAIIKKLKQMNRNDLIQALYGNK"
     gene            966..1778
                     /locus_tag="PK641_02345"
     CDS             966..1778
                     /locus_tag="PK641_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55228.1"
                     /translation="MVINNFLKQAFILLLLCIGITVSAQNNLLKDAFYELRHAENDSI
                     AISANDKFSSLLGEYLKESSAFDSSFNDLTELGTVYSDDKKVRILSWNYSLSDGRFGY
                     GTYLLYRPKTNKPTSVYYLSSNNPTKPNINYKYDANNWYGCLYYKVFAYKKMYFLLGY
                     STYNDISKIKVIDVLHISNKNISLGNEIFRLDEKSKQKQQRVVFEYSANVQMNLEYDK
                     KSKHFVFDHLSPEEPDLEGMYHYYGPDFTFDVLKLKKKVWTLQKDVDIKNKQ"
     gene            1781..3709
                     /locus_tag="PK641_02350"
     CDS             1781..3709
                     /locus_tag="PK641_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HPG55229.1"
                     /translation="MNTSKFKYKVDRFADIEVLRYLVPDFEKLTLKQKILIYHLSEAA
                     ICGRDILFDQNNRYNLWIRKTLENIYINYVGDRNCSDFLNFEEYLKRIWFSNGIHHHY
                     STNKIEPSFSEEYFEHLLKSINFEGNKSLIKSLLFDKNLYAKRVNLEQNTDLIISSAN
                     NYYRNITQKETEDFYASIINPTDTKPISYGLNSRLEKAEDGTITEKIWKLNGEYSKAI
                     EQIIAHLDSAIDYAENEKQKQIIQSLIEFYKSGDLKKFDEYSILWVEDTLSQVDFVNG
                     FIETYGDPLGCKASWESIVNFRNEEASERSIIISKNAQWFENNSPVNKKFKKEIVRGV
                     SAKVITNAMLGGDCYPATPIGINLPNSNWIRKIYGSKSVTIENIMEAYNKASYGNGFA
                     EEFYDNDQDIQAWYNYGFVTDVLHTDLHECLGHGSGQLLPGVSEDALKAYGATLEEAR
                     ADLFGLYYMADPKMIELQLLPNANAYKAAYYRYLLNGLITQSVRIPIGENIEESHMRN
                     RQLIAKYVYEKGLSEKVAELLNKNGKTYLVINDYERLRQLFGNLLSEVQRIKSEGDYE
                     AGKDLVEEYAIKINPELHKEVIERYKKLDIAPYRGFVNPVYVLKKDSKGQIIDVVPTY
                     EEGFVEQMLRYSKYYSFL"
     gene            3820..6525
                     /locus_tag="PK641_02355"
     CDS             3820..6525
                     /locus_tag="PK641_02355"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HPG55230.1"
                     /translation="MKVSFNINYNTKWGQRLYIVGSIPALGKGLYTKALPMKFVENGQ
                     WTASIDIKKPEKFTYRYFIFSDDKHFIGEWGDHREIALEAECDYFIDDTWRTNGVDKT
                     FYSSAFTKGIIARKNTAKKIVLPKSDKILQFNLPAPRVSNELSIAILGNCKELGNWNE
                     GNAVVMNDKDFPTWTASINADKLPDLVEYKYILYNVKEKIAISWESGNNRSLNTKNTD
                     IAFYIKNDEIFRYSIANWKCTGVAIPVFSLRTKNSFGIGEFSDLKLMVDWAKSTGQKI
                     IQTLPINDTTRFHTNADSYPYSSITVMGLHPIYINPFELGKLNNSAKHKKFEALRNHY
                     NESATVMYKEVAETKWEYFRLIYKQEGAKVMASKEYAKFLKENKDWLYPYAAFCFLRD
                     KNGSSTFRNWKQYSVYDKQSIENIFEDAETAEEANIHLYLQYNAHIQLLAASDYARQN
                     GVVLKGDIPIGISPDSVEAWMEPELFNLDSQAGAPPDPFSETGQNWGFPTYNWDEMER
                     DGYRWWKMRFKKMETYFQVYRIDHVLGFFRIWRMSGGDVQGLLGHFDPALPLDENEIR
                     NYGMWFEFHRMVHPYIREYILQEKFGKNASFVKSQFLELVSPDIYRFKQIFNNQRKIE
                     AYFKENKEELGLEPVLADKMYNDLLALHCEVLFIEDSKEKGKFHPRIGLHNTYSYRDL
                     DWQTKNALNRLYDDFYYHRHNDFWKTQAMKKLPALLGATDMLVCAEDLGMIPECVPNV
                     MKDLYMLSLEIERMPKDPKEEFVNMNNVPYLSVCTSSTHDMAPIRTWWNENRNLTQHY
                     YNNILGEWGTAPDNCEPWICEKIVNRHIYSPAMLVILPFQDWISINGKYRRTDANEER
                     INIPSDPHHFWCYRMHLSIEDLLDCKDLNHKILEMSNNSGRNVKM"
     gene            complement(6599..7336)
                     /locus_tag="PK641_02360"
     CDS             complement(6599..7336)
                     /locus_tag="PK641_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPG55231.1"
                     /translation="MQNFIKHRYSVRKYLSKDISNELLNELFETAFRASNTGNMQTYS
                     VIITRDVKKKQELSPCHFNQTMIMEAPVVLTFCADFRRFSKWCELSNAKPAYNNFLSF
                     FSAATDTLLAAQTFSTAAESKGLGICYLGTTNYMAQEIIDILELPSLVIPITTITLGY
                     PDEQPGQTDRLPVDGLIHYETYNDNKDIKAMYAYKESLPEMQKFVLENEKESLAQVFT
                     DIRYNKNNNEAFSKRLLEVIKNQGFLF"
     gene            7640..>9276
                     /locus_tag="PK641_02365"
     CDS             7640..>9276
                     /locus_tag="PK641_02365"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HPG55232.1"
                     /translation="MKEKKTYTFDEAMQASTEYFGGDELAAKVWINKYALKDSFGNIF
                     EKNPDDMHHRLANEIARIESNYPNPLTADQLFDLFKNFKYIVPQGSPMTGIGNTYQIA
                     SLSNCFVIGFDGESDSYGAVIKIDEEQVQLMKRRGGVGHDLSHIRPYGSPVKNSALTS
                     TGIVPFMERYSNSTREVAQDGRRGALMLSVSIKHPDSESFIDAKLEQGKVTGANVSVK
                     IHDDFMEAVLSESEYKQQYPIYSNTPKFEKTIDANALWLKIVHNAWKSAEPGVLFWDT
                     IIRESLPDCYSDLGYRTVSTNPCGEIPLCPYDSCRLIAINLYSYVENPFTKDAYFNYD
                     LFKQHSMLAQKIMDDIIDLEMEKIDKIIEKIESDPEAEEIKLTEKELWQKIRDKTVKG
                     RRTGVGTTGEGDMLAALGLRYGTEEATSFSENVHKNLALACYRSSVDMAKERGHFDVY
                     DAKREVNNPFVLRIKEADPALYDDMVKYGRRNIACLTIAPTGTTSMMTQTTSGIEPVF
                     LPVYKRRRKVNPNDAKSRVDFVDEVGDSWEEYIVFHH"
BASE COUNT         3350 a         1421 c         1639 g         2866 t
ORIGIN      
        1 tcctctaact accgaagagt tagattcgat atatgactta ccatatacaa gattgccaca
       61 tcccagatat aaaggaaaga caatacctgc ctatgagatg attaagtatt cagtaagcat
      121 tcatagagga tgctttggtg gctgttcatt ctgtacaatt tcggctcatc agggtaaatt
      181 tataagttca cgttctgaaa aatcaatttt aaaagaagta gaaaagatta ctcaagatgc
      241 tgatttcaaa ggttatgtaa gtgatttagg aggtccttct gctaatatgt acaatataca
      301 cggcaaagat atatcaattt gtcaaaagtg taaaagacca tcttgtttac atccacatat
      361 atgcaataat ttacagacta atcacgaaag aattattaat ttatatcgac aagcaaataa
      421 aattaacgga ataaaaaaga tctttatcgg tagtggtatt agatacgatt tagcaacaac
      481 agattattta gaggaagtag ttaaacatca tgtttcaggt aggcttaaag tcgctcctga
      541 acacactaat gacgcagtac ttaatttgat gcgtaaacct tcattcaagt tttttgaagc
      601 atttgataag gaatttgaaa gaatcaacaa agagtccggt ttacgtcaac agttgatccc
      661 atattttatt tcaagtcatc ctggatgtac gacagagcaa atggcagaat tagcaagtaa
      721 aacaaagcgt atgcactttc aactggaaca ggtacaagat tttacgccaa ctcctatgac
      781 actatcaacc gaaatttatt ataccggctt aaatccatat acattagaga aagtttatac
      841 ggctaacact ccggcactaa aattagaaca acgtagtttc tttttttggt ataaacctga
      901 agaaagagct gctataataa agaaactaaa acaaatgaat cgtaatgatt taatacaagc
      961 attgtatggt aataaataat tttttaaaac aagcatttat acttttattg ttatgtatcg
     1021 gcattacagt ttctgcgcaa aataacttat taaaagatgc gttttatgag ttaagacatg
     1081 cagaaaatga tagcatagct atatcggcca atgacaaatt ctcgtctctg ctgggagaat
     1141 atttaaaaga aagttctgca ttcgactcat cattcaatga tttgactgaa ttaggaacag
     1201 tatattctga tgataagaaa gtcagaatac tttcatggaa ttattcactt tctgatggcc
     1261 gttttggcta tggaacatat ctattatata gaccaaaaac aaataaaccg acatccgttt
     1321 actacctgtc ttcaaataat ccaacaaaac caaatattaa ctataaatac gatgcgaata
     1381 attggtatgg atgtttgtat tacaaggtat tcgcttataa aaaaatgtat ttccttttag
     1441 gatacagcac ttataatgat attagtaaaa ttaaagtaat tgatgtcttg cacatcagta
     1501 ataaaaacat ttctttgggc aacgaaatct tcagacttga tgaaaaatca aagcaaaaac
     1561 aacaaagagt cgtttttgag tatagtgcca atgttcaaat gaatttagaa tacgacaaaa
     1621 aaagcaagca ttttgtattt gatcatttat cccccgaaga acctgattta gaaggtatgt
     1681 atcattatta tggtcccgac tttactttcg atgttttaaa actaaaaaaa aaagtgtgga
     1741 ctctacaaaa agacgtggat ataaaaaaca aacaatagaa atgaatacaa gcaaattcaa
     1801 atataaagtt gatagatttg ccgatataga agtgcttagg tatcttgttc cggattttga
     1861 aaaacttaca ctgaaacaaa aaatattgat atatcatttg tcagaagcag caatatgtgg
     1921 tcgtgatata ttatttgatc aaaataaccg atataatcta tggattcgta aaaccttaga
     1981 aaatatctat attaattatg tcggtgatcg taattgttct gatttcttga attttgaaga
     2041 atatttgaag cgcatttggt tttctaacgg aatacatcac cactattcta ccaataaaat
     2101 agagccatca tttagtgagg aatattttga gcatctctta aagtcaatca attttgaagg
     2161 taataaaagc ttaataaagt cattgctatt cgataaaaac ctatatgcta aaagggttaa
     2221 tcttgaacag aataccgatt taataatcag ttcagctaac aattactaca gaaatataac
     2281 acaaaaagaa acggaagatt tctatgcatc tattataaat cctactgata caaagcccat
     2341 ctcttatggc ttaaacagcc gccttgaaaa agcagaagat ggcactatta ccgaaaagat
     2401 atggaaacta aatggagagt attcaaaagc tattgaacaa atcatagctc acttagatag
     2461 tgcaatagat tatgcagaaa acgaaaaaca aaagcaaata atacaaagtt taatagagtt
     2521 ttacaaaagt ggcgatttaa agaaatttga cgaatattca atcttgtggg tagaagacac
     2581 tttatcgcaa gtagattttg taaacggatt catagaaact tacggagatc ctctcggatg
     2641 taaagcgagt tgggaatcta tagtaaattt tagaaacgaa gaagcttctg aacgatctat
     2701 tattataagt aagaatgctc aatggtttga aaacaattct cctgtaaata aaaaatttaa
     2761 aaaagaaata gtacgtggag tttctgcaaa agttattaca aatgcgatgc ttggaggcga
     2821 ttgctaccct gctactccaa taggaattaa tttgccaaac tcaaattgga ttcgaaaaat
     2881 atatggttca aaatcagtta ctatagaaaa tatcatggag gcttacaata aagcatctta
     2941 tggcaatgga tttgctgaag aattttatga taacgatcag gatatacaag catggtataa
     3001 ttatggtttt gttactgatg tcttacatac tgatttacac gaatgtttag gacatggatc
     3061 agggcaatta ctacccggcg tttcagaaga tgctttaaaa gcctatggtg caacattaga
     3121 agaagcaaga gcagatctgt ttggactata ttatatggca gatcctaaaa tgatagaact
     3181 tcaattatta cctaatgcaa atgcctataa agctgcgtat tacagatatt tattaaatgg
     3241 tttaattaca caatcggttc gcatccctat tggcgaaaat atagaggaat ctcatatgcg
     3301 taatcgccaa ttaatagcta aatatgtata cgaaaagggt ttgtctgaga aagttgcaga
     3361 actgcttaat aaaaacggaa aaacctatct tgtgattaat gattatgagc gtttaagaca
     3421 attatttggc aatttattgt ccgaagtaca aagaattaaa tcagaaggag attatgaagc
     3481 tggcaaagat ttggtcgaag aatatgcaat caaaataaat cccgaacttc ataaagaagt
     3541 catagagcga tataaaaaat tagacatcgc cccttatcgt ggttttgtta accctgtata
     3601 tgtattaaaa aaagattcaa aaggacagat tattgatgta gttccaacat acgaagaagg
     3661 ctttgtagag cagatgttaa ggtactccaa atattactcc ttcttgtaac gtttgcgtag
     3721 tttttttggt caattttaga tgacaaaaac ctttatgtaa aggtagaatt gctatatttg
     3781 cttgttaaac aaacttattt gcaataccta aacaaaccta tgaaagtctc ttttaacatc
     3841 aattacaaca caaaatgggg ccaaaggcta tatattgtcg gctcaattcc cgctttaggc
     3901 aagggtttat acacgaaggc tctacctatg aagtttgtcg aaaatggtca gtggaccgca
     3961 tcaatcgaca taaaaaaacc ggagaaattc acttatagat attttatttt tagcgatgat
     4021 aagcatttta tcggtgaatg gggagaccat agagaaatag cattggaagc agaatgcgac
     4081 tacttcattg acgacacatg gcgcacgaat ggtgttgata agacattcta ttcatctgca
     4141 tttactaaag gtattatagc aagaaagaat acggcaaaaa aaatcgtatt accaaaatca
     4201 gacaaaatat tacaatttaa cctacctgca ccaagggtat ctaacgaatt atcaattgct
     4261 attttaggca attgcaaaga attaggaaat tggaatgaag gtaatgctgt tgtaatgaac
     4321 gacaaagact tccctacttg gactgcttca attaatgccg ataaattacc tgatttagtt
     4381 gagtataaat acattcttta taacgtaaaa gaaaagattg ctatatcatg ggaaagtggc
     4441 aataatcgta gtttaaacac taaaaataca gatattgcat tttacattaa aaatgatgaa
     4501 atttttaggt attcaatagc aaattggaaa tgtacaggag ttgcgatacc tgttttctct
     4561 ttaagaacta aaaatagttt cggtataggt gaattctctg atttaaaatt aatggttgat
     4621 tgggcaaaat caacgggtca aaaaataata caaaccttac ctattaacga caccacacgt
     4681 tttcacacaa atgccgattc atatccgtat agtagtatca ctgtaatggg tttgcatcct
     4741 atatacatta atccatttga actaggtaaa ttaaacaata gcgcaaaaca caaaaagttt
     4801 gaggcactca gaaatcatta taatgagagc gccacagtta tgtataaaga agttgcagaa
     4861 acaaaatggg aatatttccg tttaatatat aagcaagaag gtgcaaaagt gatggctagt
     4921 aaagaatatg caaaattctt gaaggaaaac aaagattggt tatatcctta tgcggcattc
     4981 tgcttcttac gtgataaaaa tggtagttca actttccgca attggaaaca atactcggtt
     5041 tacgacaagc agagcataga aaatattttt gaagatgcag aaactgccga agaagctaat
     5101 atacatttat atcttcaata taatgcacac attcaattat tggctgcatc agattatgca
     5161 cgtcaaaatg gtgtggtatt aaaaggcgat attcctatcg gaattagtcc tgatagcgtg
     5221 gaagcttgga tggaacctga attattcaac cttgatagcc aagcaggtgc accacctgat
     5281 ccattctcag aaacaggtca aaactgggga ttcccaacct acaattggga tgaaatggag
     5341 cgtgacgggt atcgttggtg gaaaatgcgt ttcaagaaga tggaaactta tttccaagta
     5401 tatcgaattg accatgtgct gggcttcttt agaatatggc gcatgtcagg tggtgatgta
     5461 caaggtttgt taggacattt cgacccggca ctcccactcg acgaaaacga aattcgtaat
     5521 tacggaatgt ggtttgagtt tcatcgtatg gtacatccat acattcgtga atatattttg
     5581 caagagaaat ttggcaaaaa cgcaagtttt gtaaaatctc aatttttaga actagtttca
     5641 cctgatatat atcgcttcaa acagatattc aataaccaga gaaagattga agcatacttt
     5701 aaagaaaata aagaagaatt aggattggaa cctgtattag ccgataaaat gtacaatgat
     5761 ttgttggctt tacattgcga ggtattattt attgaagata gtaaagaaaa aggtaaattt
     5821 catcctcgta tcggtttaca caatacatat tcttacagag atttagattg gcaaacaaag
     5881 aatgcattaa atcgtttata tgatgatttc tattatcatc gtcataacga tttctggaag
     5941 acgcaagcta tgaagaaatt gcctgcatta ttaggtgcta cagatatgtt ggtgtgtgca
     6001 gaagatttag gtatgattcc ggaatgtgta cctaatgtaa tgaaagattt gtatatgttg
     6061 agtttggaga ttgaacgtat gccaaaagat ccaaaagaag aatttgtaaa tatgaataat
     6121 gttccatatt tgtcagtttg cacatcttca actcacgata tggctcctat tcgtacttgg
     6181 tggaatgaga atagaaactt aactcaacat tactataata atatattagg cgaatgggga
     6241 actgctcctg acaattgtga gccatggata tgcgagaaaa tagttaatag acacatttac
     6301 tctcctgcta tgcttgtgat actgccattt caagattgga tttcaattaa tggaaaatat
     6361 cgcagaacgg atgcaaatga agagcgtatc aatatcccat cagatccaca tcatttctgg
     6421 tgttacagaa tgcatttatc aatagaagat ttattagatt gcaaagactt gaatcacaaa
     6481 atattagaga tgtctaataa tagtggtcgt aacgtaaaaa tgtaattaaa aacatcaaaa
     6541 ttaaagccgg tacatatata atttatgtat cggctttttt attgaactat attaattctt
     6601 aaaacaaaaa accttgattc ttaataacct ccaaaagtct tttagaaaaa gcttcgttat
     6661 tatttttatt ataccttatg tcagtaaata cttgagctaa actttctttt tcattttcaa
     6721 gaacaaattt ctgcatttca ggtaatgatt ctttataagc gtacattgct ttaatatcct
     6781 tattatcatt gtatgtctca taatgaatca atccgtctac aggaagtctg tctgtctgcc
     6841 ctggctgctc atcaggatag cctaaagtaa tagttgtaat aggaatcact aatgatggta
     6901 attctaaaat atcaataatc tcttgcgcca tataattagt tgttccgaga taacaaatac
     6961 ccaagccttt agattctgct gcagtagaaa atgtttgagc agctaacaat gtatctgtag
     7021 ctgccgaaaa aaatgacaaa aaattattat atgccggctt agcattacta agttcgcacc
     7081 attttgaaaa acgtctaaaa tctgcacaaa aagtcaaaac aacaggagcc tccataatca
     7141 ttgtttgatt aaagtgacat ggcgataatt cttgcttttt tttgacatcg cgagttatta
     7201 ttacgctata tgtctgcata ttacctgtgt ttgatgctct aaatgctgtt tcaaacagtt
     7261 cgtttaataa ctcattcgaa atatctttag ataaatattt tcttactgag tatctgtgtt
     7321 ttataaaatt ttgcataata atacattgtt tatttgtcaa atatatgaaa agtcattgaa
     7381 tttacaaaat atttttttag aaaacttttt aaatagtcga attttatata gttctaatta
     7441 tcaggaattt agaaaatatt ttagaaaatt tcacaattaa tattcatttt gattgtttat
     7501 catttgtgta aaaatttaat gaaaaatatt tgctacgata aaaatttatg cctaacattg
     7561 caatctgtta gtgtgaaatt tattcgaaat aaaatatagt gattaataac aaaatattta
     7621 acctcataca aataacatta tgaaagaaaa gaaaacctac acttttgacg aagcgatgca
     7681 agcatccacc gaatattttg gaggggatga gttggctgca aaggtttgga ttaacaagta
     7741 tgccctaaaa gattctttcg gcaatatatt tgagaaaaat cctgacgata tgcaccatcg
     7801 tttggcgaat gagattgccc gtattgagtc aaattaccct aacccactta ctgcagacca
     7861 attatttgat ttgttcaaaa actttaagta cattgttccg caagggagtc caatgaccgg
     7921 aattggaaac acgtatcaaa ttgcatcctt atcaaattgc tttgtaattg gtttcgatgg
     7981 cgaatccgat tcttatggtg cagttatcaa aattgatgaa gaacaagtac aattgatgaa
     8041 acgccgtggt ggtgttggtc acgatttatc tcatattcgt ccttatggtt cgcctgtaaa
     8101 aaattcagca cttacatcaa caggtattgt gccttttatg gaaagatact caaattctac
     8161 acgtgaggtt gcacaagatg gtcgtcgtgg agctttaatg ttaagtgtat ctattaaaca
     8221 tcctgattca gaatccttta tagatgcaaa attggagcaa ggcaaggtaa ccggcgctaa
     8281 tgtttcagta aaaatccatg atgattttat ggaagcagta ttgtctgaaa gcgaatataa
     8341 acaacaatac cctatctatt cgaacactcc taaatttgag aagacaattg atgccaatgc
     8401 tttatggcta aaaattgtac acaatgcatg gaaatcagct gaaccgggag ttttgttctg
     8461 ggatacaatt attcgcgaat cattacctga ttgctatagt gatcttggtt accgtaccgt
     8521 atcaacaaac ccatgtggag aaatccctct atgtccgtac gatagctgta gattaattgc
     8581 tataaattta tattcttatg ttgagaatcc atttacaaaa gatgcttatt ttaattatga
     8641 tttatttaag caacactcaa tgttggcaca aaagataatg gatgatatta tagaccttga
     8701 gatggagaaa attgataaga ttatagaaaa aatcgaatcc gatcctgaag cagaagaaat
     8761 aaaactaaca gaaaaagaat tgtggcaaaa aatccgcgac aaaacagtaa aaggacgtcg
     8821 tacaggtgtt ggaacaactg gtgaaggcga tatgttagct gctttaggtt tacgatatgg
     8881 aacagaagaa gctacatctt tctctgaaaa tgtacataaa aatttagcat tagcatgtta
     8941 tcgctcttca gttgatatgg ctaaggaacg tggacatttt gatgtatacg atgcaaaaag
     9001 agaagttaac aatccttttg tactcagaat aaaagaagca gatcccgcac tatatgatga
     9061 tatggttaaa tatggtcgtc gtaatattgc ttgtttaaca attgctccaa caggtactac
     9121 aagtatgatg acacagacca catcaggtat agaacctgta tttttacctg tgtacaaacg
     9181 tagacgtaag gttaatccta atgatgcaaa atcacgtgtt gattttgttg acgaagttgg
     9241 tgatagctgg gaagaatata ttgtatttca tcataa
//
LOCUS       DAOPID010000037         9245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_9339_length_9245_cov_4.879543, whole genome shotgun sequence.
ACCESSION   DAOPID010000037 DAOPID010000000
VERSION     DAOPID010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 9245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9245
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9339_length_9245_cov_4.879543"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..852
                     /locus_tag="PK641_02370"
                     /pseudo
     CDS             70..852
                     /locus_tag="PK641_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260654.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
     gene            1185..2531
                     /locus_tag="PK641_02375"
     CDS             1185..2531
                     /locus_tag="PK641_02375"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPG55233.1"
                     /translation="MAHLLKRKIDTFLAEWKHNNAHLPLIVKGARQVGKTASIMAFAE
                     ANYESVIAINFALQPKFKNICSDGFDVDSILKNISFLLPDSKLPQKKTLIFFDEIQAY
                     SACATSLKSFALDGNYDVICSGSLMGINYNEIESNSVGYKEDYEMHSMDFEEFLWAKS
                     YSAQQIEGLFNKMIELKPLSTVEVSVLSDIFRDYMITGGMPAIVNNYIKNNNFSDTLG
                     IQKQLLLDYEEDITKYAIGLDKGKIKSIYNHISIFLGKDNKKFQITKIAKNARNRDYI
                     GTVDWLNDAGIINICYCLDSVELPLKGNYNPKSYKLYYKDTGLLIAALDDESQEDLRA
                     NRNFNTYKGAIYENIVGDMLVKQGYQLYFYKNESSNLEMDFFVRDRDSLIPIEVKAND
                     NSTRSLNNLLDDNIKFKDIKFGIKLCNRNIGFNGKFYTFPYFLSFMIKRFLQDRNQ"
     gene            3147..3818
                     /locus_tag="PK641_02380"
     CDS             3147..3818
                     /locus_tag="PK641_02380"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HPG55234.1"
                     /translation="MEKYIIIVAGGTGNRMGANVPKQFLPLNGEPILACTLRAFANWG
                     NIILVLPETQMTYWQSLCKNLRDMPDYKMVAGGEQRFYSVYNALQFVPDGALVAVHDG
                     VRPLVSQTCINRVFTLAEEMGSALPVINCVDSLRHISNGSSLSVNRNEYVCVQTPQVF
                     NSTNLKKAYQQPYKECFTDDASVYESASYKINLCDGNIENIKITTPIDLIHAEAILKN
                     GFDKV"
     gene            3799..5886
                     /gene="recG"
                     /locus_tag="PK641_02385"
     CDS             3799..5886
                     /gene="recG"
                     /locus_tag="PK641_02385"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HPG55235.1"
                     /translation="MDLTKSDIKYLHGMGPKRAELLNKELEIYTLDDLVHYFPYKYID
                     RSRFYQISEIDSTAAYIQIKGQILGFKEAGKGRAQRLIAIFTDGHSSIELIFFKGIKY
                     VMDAYHLNTDYIVFGKPNFFNGNYSFVHPDIEKPTPDSMTGFMPLYHTTEKMKNKFLN
                     SKAIQKLIYTAVKQMDKAIPETLPEYIISKYHLMDLYTSLVQVHFPQNMNILDKARYR
                     LKFEELFYIQLAILRQANVFKSKVCGHEFKQIGKYFNSFYHERLPFELTEAQKRVLRE
                     IREDVRNGKQMNRLLQGDVGSGKTIVALLSMLMAVDNGFQACIVAPTEILATQHYEEM
                     CRLLEGIGVEIRLLTGSTKKKAREELHAKLRDGSLSILVGTHAILEDCVVFKDLGLVV
                     IDEQHRFGVAQRARLWAKNLIIPHVLVMTATPIPRTLAMTLYGDLDVSVIDELPPGRK
                     PVQTDHIYEDKRGLMYRFLRRQMEAGRQIYVVFPLIQESEKSDLKDLESGFESLSKEF
                     SEFAISMVHGKMKPAEKDAEMQKFVSGETKMLVATTVIEVGVNVPNASVMVIENAERF
                     GLSQLHQLRGRVGRGAEQSFCLLMTKYELSSDTRKRIDIMTETNDGFLIAETDLKLRG
                     PGDLEGTMQSGLPFDLRIADLTKDGPILQTARDAASAVLAEDIMLSSAANSILSTQLK
                     KRSKEIVNWSAIS"
     gene            5994..6455
                     /gene="ndk"
                     /locus_tag="PK641_02390"
     CDS             5994..6455
                     /gene="ndk"
                     /locus_tag="PK641_02390"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006281370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HPG55236.1"
                     /translation="MERTLVILKPSAIARSLVGEVISRFERKGLQLAGIKMIQLSDEI
                     LNEHYAHLLDKPFFPRIKASMQASPVIVCCWEGLDAVQIVRDMTGVTNGRKAVVGTIR
                     GDFSVSMQENIVHTSDSPENAVVELNRFFSPNELFAYENPIKCFRYANDEV"
     gene            6470..7357
                     /locus_tag="PK641_02395"
     CDS             6470..7357
                     /locus_tag="PK641_02395"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPG55237.1"
                     /translation="MDVVKRIKFLALLAGVASVSFAEPSTDNIFGIEEIEISDVDAGE
                     SQLLPPDTEDDLTDFSEFGSYDENLPEGLYNSIWSDVAVNPYNINLYKMKDTVSIDMR
                     DYHHPIYGRVTSVFGMRRYRYHYGTDIKLNIGDTVRSAFDGKVRIAKIGRGYGYYVLV
                     RHDNGLETIYGHLSKILVEPESAVCAGDAIALGGNTGRSTGPHLHFEMRYVGNPINPQ
                     TLVDFETGRPIKDTYLVCAESFKYKGNINNLAYYTVRRGDTLSGIAKRRGTTVSKLCK
                     LNNITARTTLKIGRKLRCS"
     gene            7382..>9245
                     /gene="secA"
                     /locus_tag="PK641_02400"
     CDS             7382..>9245
                     /gene="secA"
                     /locus_tag="PK641_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007366137.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; in some organisms, there are paralogous
                     proteins that have been found to be nonessential but do
                     function in secretion of a subset of exported proteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPG55238.1"
                     /translation="MNIGSIIGKLFGTKSERDLKEILPYVEKIKVVYPSIEKLSNDEL
                     RERTQNIRAEIKNSAASEYAKIAELKESIDSTDLQDREKLYAEIDKLESQALDNISAA
                     LDTFLPEVFSIIKDTARRFKENSDIVVTANEFDKQLAVNHDFVDIDGDKAIYHNEWTA
                     GGNTIKWDMVHYDVQLIGGVALHKGKIAEMATGEGKTLVATLPVFLNALSGNGVHIVT
                     VNDYLSKRDSEWMGPLYMFHGLSVDCIDKHKPNSDERRKAYNSDITFGTNNEFGFDYL
                     RDNMASSPKDLVQRMHNYAIVDEVDSVLIDDARTPLIISGPVPKGEDQLFEEYRPKVE
                     KIVAAQKALATKLLADAKRLMASENEKEREQGAIYLFRTYKGLPKNNPLIKFLSEPGV
                     KATMLKTESVYMEQNDRRMHEITDDLYFVINEQHNSIELTDKGIDLITGDTDDAKFFV
                     LPDIGSELADLEKGNISEEEKISRKDALMQEYAVKSERVHTVNQLLKAYTLFEKDDEY
                     VVIDNKVMIVDEQTGRIMEGRRYSDGLHQAIEAKERVTVEAATQTFATITLQNYFRMY
                     RKLAGMTGTAETEAGEFWDIYKLDVVVIPTNRPIVRNDMNDRVYKTKRVKYNAVI"
BASE COUNT         3071 a         1486 c         1930 g         2758 t
ORIGIN      
        1 aaaaccgcat gtatctcgtt atgaggcaca tgcggttttt gtataacact ttattaaata
       61 ggcaataaca tggcaatata cgacgataat aatttaagag atactttaga atatctttat
      121 gataaatata atcgtgagga atttatagag cacgatccta taagcatacc acataaattt
      181 gataggcaag aggatataga aataagtggg tttttagcag cagccattgc atggggcaat
      241 cgtaaggcaa tagtaaaaag tgcaaacagg atgcttacat atttagataa taagccatac
      301 gattttgttc taaacgcatc tgatgcagaa atcaaaaact tatactcatt tgtacataga
      361 acatttaatg gtcgggactt cgctgatttt attaagtcac tcagacattt ctaccttaaa
      421 tacggcagcc taggaaattt ctttgaagaa aattatttgc agagcaatga tattcgcatt
      481 gtactgtcaa aattcagagc tgaatttatg tgtgtagaac acaatgatca ttgcgataaa
      541 catatttctt caatagataa agcttcagct tgcaaaaggc ttaatatgta tttacgttgg
      601 atggtacgtc gtgatgatag aagcgttgat tttggattat ggaaacgcat acccatgtcg
      661 agcctatata ttccattaga tgtacatagc ggcaacacag gacgcgaatt aggcgtattg
      721 cagcgcactc agaacgattg gaaagcagta gaataattaa caggcagttt acgtaaattc
      781 gatgcatccg accccgttcg ttttgatttt gccctgtttg gagtgggggt tgataaaaga
      841 gtgaatggtt aagggcgaat gcaattcgcc cttacacatt acacgattac acattacacg
      901 attccacatt acaccttaca cgattccacg ccctctaacc cctcaccctt caccctacac
      961 attacacact acacattaca cgattccaca ttacacctta cacgattaca cactacacac
     1021 ccttaaccca tattttcgaa aaatgtgaaa aatcacactt aaaagcgtct aaaaatgtga
     1081 aaaattacac atataagcgt cgaaaaatgt gaaattaatg gcgtaaaact ttggaaaaat
     1141 gtgaaaaatg agtatttttg tattcatatt caatcagtta ccatatggca cacctactaa
     1201 agcgaaaaat tgatacattc cttgcagagt ggaaacataa caacgctcat ttgccgctaa
     1261 tagtaaaagg cgcacggcaa gttggcaaaa cagcatcaat aatggcattt gccgaagcaa
     1321 attacgaaag cgttattgct attaattttg cgttacagcc gaaatttaaa aatatctgta
     1381 gtgatggatt tgacgttgat agcattctga aaaatatttc gtttcttttg cccgattcta
     1441 aactgcccca aaagaaaaca ctcatttttt tcgacgaaat tcaagcatat tctgcatgtg
     1501 caacaagttt aaaatcattt gcgttagatg gaaattatga tgttatttgt tccggttctt
     1561 taatgggaat aaactataac gaaattgaat ctaacagtgt tggatataaa gaagattatg
     1621 aaatgcactc aatggatttt gaggagtttt tatgggcaaa atcgtatagt gcgcaacaaa
     1681 tagaaggctt attcaataaa atgatagaac ttaagccgtt aagtactgtc gaagttagtg
     1741 ttttgtcaga tatttttaga gattatatga ttaccggcgg catgcctgcc attgtaaaca
     1801 attatattaa aaacaataac tttagtgata cgctgggaat acaaaaacaa ttgcttttag
     1861 attatgaaga agatattact aaatatgcta tcggattaga taagggaaag atcaaaagta
     1921 tttataatca catatcaatt tttttaggta aagacaataa aaaatttcag atcaccaaga
     1981 ttgcaaagaa tgctcgtaat agagactata ttggaaccgt agattggctt aatgatgcag
     2041 gaattataaa tatctgttat tgtcttgata gcgttgaact acctcttaag ggtaattata
     2101 atccgaagtc atacaaactg tattacaaag ataccggttt acttatagca gcattagatg
     2161 atgagtcgca ggaagattta cgagcaaata gaaactttaa tacttacaaa ggagctattt
     2221 acgaaaatat tgttggagat atgcttgtta aacaagggta tcagctttat ttttataaaa
     2281 atgaaagttc taatttagaa atggattttt ttgttagaga tagggattca cttattccta
     2341 ttgaggtaaa ggcaaatgat aattcaacaa gatctcttaa taatttgctt gatgataata
     2401 taaaattcaa ggacattaag ttcgggataa agttgtgtaa taggaatatc ggatttaatg
     2461 gtaaattcta cactttcccg tattttttat cctttatgat taagcgtttt ttgcaagacc
     2521 gcaatcaata atcttcccat ccattccaca ctccacgccc ttcactaggc tgttgtgtag
     2581 ggtgaaaaag ggtgaggggt taggggttag gggtgaaggg ttaggggtta agggttaagg
     2641 ggtgtgtaat cgtgtaatgt gtagtgtgta aatccaatgt tgaaatgatc aataatgcgt
     2701 tgtaggggcg aattgcattc gcccttcaca ttcgacatgc acaaatattt cgaaatgaat
     2761 aaccgcaaca caatgcgacc gtaggggcat gccatggcat gcccttcgca taaaatacgc
     2821 gacgatttat cgaacaacgt gtatattaat tatacatttc aacattgtat ttatatttat
     2881 cgcattgtga attcgaaggg cgaatgcaat tcgcccctac gggtgcgttg cggattattt
     2941 caacattgga tttatattaa tcacatgtcg aaaatgaagg gcacgccatg gcatgcccct
     3001 acaacgcatt attgattatt tcaacatttg attatacacg attacacgat tacacgatta
     3061 cacgattaca cgattacacg attacacgat tccacactac acattctaag aaaaaaatgt
     3121 aactttgcgt ttaataacga aagtctatgg aaaaatatat cattattgtg gcgggtggaa
     3181 cagggaatag gatgggagcg aatgttccca aacaatttct gcctctaaat ggagaaccaa
     3241 ttttagcttg tacattaaga gcatttgcaa attggggaaa tatcattttg gttttgcctg
     3301 aaacacagat gacttattgg caatctttgt gtaaaaattt gcgagatatg cccgattata
     3361 aaatggtagc aggaggagag caacgttttt attcggtgta caatgcactt caatttgttc
     3421 ccgatggtgc attagtcgcc gtacatgacg gagttcgtcc tttggtgtct caaacctgca
     3481 tcaatcgagt atttacatta gcagaagaaa tgggatctgc actaccggtt ataaattgtg
     3541 tcgattcatt acgtcatatt agtaacggga gcagcctgtc tgttaataga aacgagtatg
     3601 tttgcgtaca gactccgcaa gtatttaatt ctacaaattt gaagaaagca tatcagcagc
     3661 catataagga atgtttcact gatgatgcat ctgtctatga atcagcatca tacaaaatta
     3721 atctgtgtga tggaaatatc gaaaatatta aaataaccac gcctatcgat ttaattcatg
     3781 ctgaagcaat attgaagaat ggatttgaca aagtctgaca taaaatattt gcatggtatg
     3841 ggtccgaaac gtgccgaatt actgaacaag gaattagaaa tatatacctt ggacgatttg
     3901 gtgcattatt ttccgtataa atacatagac cgaagtcgat tttatcagat cagtgagata
     3961 gattcgacag cagcatatat tcagattaaa gggcaaatac tcggttttaa agaagccggc
     4021 aagggtaggg ctcaacgtct gattgctatc tttacagacg gacacagcag catagaactg
     4081 atatttttta agggtattaa gtatgtgatg gatgcctatc acttgaatac tgattacatt
     4141 gttttcggta agcccaattt ctttaacggc aactacagtt ttgtgcatcc tgatatagaa
     4201 aaacctactc ccgacagtat gacaggtttt atgccgctat atcatacaac agaaaaaatg
     4261 aagaataagt tcttgaattc aaaagcaatt cagaaactta tatatactgc cgtaaaacag
     4321 atggataagg cgattcctga aacattgccc gaatacatca tctccaagta tcatctaatg
     4381 gatttgtaca cttctttggt gcaagtgcat ttccctcaga atatgaacat acttgataag
     4441 gcacgataca gacttaaatt tgaggagttg ttttatattc aacttgcaat tttaaggcag
     4501 gctaatgtgt ttaagtcgaa ggtatgcgga catgaattta agcagatagg aaagtatttt
     4561 aatagttttt atcacgagcg tttgcctttt gagttgacgg aggcgcaaaa aagagtattg
     4621 cgcgaaataa gggaagatgt acgaaatggt aagcagatga atcgtttgtt gcaaggggat
     4681 gtaggcagtg gtaaaacaat tgtagctctg ctatctatgc taatggctgt tgataatggt
     4741 ttccaagcat gtattgtggc tccaaccgaa atattagcaa ctcaacatta tgaagaaatg
     4801 tgtaggttgc tcgaaggtat aggtgttgaa ataagattat tgacaggatc gaccaagaag
     4861 aaagcgcgcg aagagttgca tgcaaaactt agagacggaa gtttgtcaat cttagttggt
     4921 acgcatgcaa ttttagaaga ttgtgtggtg tttaaggatc tgggtttagt agttattgac
     4981 gaacaacata ggtttggagt agctcaaaga gccagattat gggcaaagaa tttgataata
     5041 cctcatgttt tggtaatgac agcaacgcct attccacgta ctttggctat gacattatac
     5101 ggcgatcttg atgtatctgt aatagacgaa ttacctcccg gcaggaaacc tgtgcaaaca
     5161 gatcatattt atgaagacaa acgtggattg atgtataggt ttttgcgtag gcagatggag
     5221 gcaggacgtc aaatatacgt ggtattcccg ctgatacagg aatcagaaaa atctgattta
     5281 aaggacctgg aaagcggctt tgaaagctta agcaaggagt tttcggaatt tgcaataagt
     5341 atggtgcacg gcaagatgaa acctgctgaa aaagatgctg aaatgcagaa atttgtaagc
     5401 ggtgaaacaa aaatgcttgt agctacaact gtaatagaag tgggagttaa tgtgcctaat
     5461 gcatcagtta tggtaattga aaatgccgag cgtttcggtt tgtcgcaatt acatcaactt
     5521 cgtggacgag taggtcgtgg agcagaacaa tcattttgct tgttgatgac taaatacgaa
     5581 ttatcttcgg atacccgaaa acgtatagat atcatgacag agacaaatga tggattttta
     5641 atagccgaga cagacttgaa attacgcggc cccggcgatt tggaaggtac tatgcagagc
     5701 ggacttccat ttgatttaag aatagccgat ttaactaaag acggccctat tttacaaacc
     5761 gcaagagatg ctgcatcagc cgtattggct gaagatatta tgctgagttc ggctgcaaat
     5821 tcaatcttat cgacacaact taaaaaacgc tcaaaagaga tcgtaaattg gagtgcgatt
     5881 agctgatagt gtctgcatca gccgtattat acattcttta acaacaaaac aagtttatta
     5941 tcctcatttg tcggtaaaaa tcattatttt tgtgacttta atcacaaaaa cacatggaac
     6001 gtacccttgt cattctaaaa cctagtgcca tagcacgcag tttggtcggt gaagtcatca
     6061 gccgtttcga gcgtaaaggc ttgcaacttg caggaatcaa aatgattcag ctttctgacg
     6121 aaattttaaa cgagcattat gctcatttgt tggataagcc gttttttccg cgtattaaag
     6181 cgtcaatgca ggctagtccg gtgattgtgt gttgctggga aggtttagat gcggtacaga
     6241 ttgtgcgcga tatgacaggt gttacaaatg gtcgtaaggc tgttgtcgga acaatcagag
     6301 gcgatttttc tgttagtatg caggaaaata ttgtacatac atccgattct ccagaaaatg
     6361 cagttgtcga attaaatcga tttttttctc caaacgaatt atttgcatac gaaaatccaa
     6421 tcaaatgttt ccgttatgca aatgacgagg tttgataaaa tattttttga tggatgttgt
     6481 aaaaagaatt aagtttttgg cattgcttgc cggtgtggcc tcagtatctt tcgcagagcc
     6541 ctcaacggat aatatctttg gcatagaaga gatagaaata agtgatgtag atgccggtga
     6601 aagtcaatta ttaccccccg acactgaaga tgatttaacc gacttcagtg aatttggctc
     6661 gtacgatgag aatttacccg aaggtttgta taattctatc tggagcgatg tagcagtaaa
     6721 tccgtataac ataaatctgt ataagatgaa agataccgtg tcaatagaca tgcgggatta
     6781 tcatcatcct atctacgggc gagtaacatc tgtgtttggt atgcgaagat atcgttatca
     6841 ctatggcaca gatataaaat tgaatatagg agatactgta agaagcgctt ttgacggtaa
     6901 ggtaagaata gcaaaaatag gtagaggcta tggctattac gtacttgtac gtcatgataa
     6961 tggacttgag accatatatg gtcatttatc aaaaatattg gtagaacccg aaagtgcagt
     7021 ttgtgcgggt gatgctatcg ctttgggcgg aaataccgga cgtagtacag gccctcattt
     7081 acattttgaa atgcgttatg tagggaatcc gattaatcct caaacattgg tagatttcga
     7141 aacgggtagg cctataaaag atacatatct tgtttgcgcc gaatcattta aatataaggg
     7201 caatataaat aatttggcat attacacagt aagacgtggc gatacattgt ccggtattgc
     7261 taagagaaga ggaacgactg tttctaaatt gtgtaagctg aataatatta cagcaagaac
     7321 tactttgaaa atcggacgca aattgcgttg ttcatagaat taaaattaaa ctattacaat
     7381 aatgaatatt ggatctatta tagggaagtt gtttggcaca aaatcggaac gagatttaaa
     7441 agaaatattg ccttatgtcg aaaagattaa ggttgtgtat ccctcaattg aaaaattaag
     7501 caacgacgag ttacgtgaac gtacacaaaa tatcagagcc gaaattaaaa atagtgctgc
     7561 atcggaatat gcaaaaattg cagaacttaa agaaagcatt gactcaactg atttacaaga
     7621 tcgtgaaaaa ttatacgccg agatagataa attagagagt caggcattag ataatatttc
     7681 agctgcttta gatactttct tgcctgaggt tttctctatc attaaagata cagcaagacg
     7741 ttttaaagaa aattccgata tcgtggtaac tgccaatgaa tttgacaaac aattggctgt
     7801 aaatcacgat tttgttgata ttgacggtga caaagctata tatcataacg aatggactgc
     7861 aggtggaaac actatcaaat gggatatggt acattacgat gtgcagttga ttggtggtgt
     7921 tgctcttcac aaaggtaaaa ttgctgaaat ggcaaccggt gaaggtaaaa ctttagttgc
     7981 tactttgcct gtgttcttaa atgctcttag cggaaacggt gtgcatatag taactgtaaa
     8041 tgattatttg tcaaaacgtg actctgaatg gatggggcct ttatacatgt tccatggatt
     8101 gagtgtggat tgtatcgaca aacataaacc aaactctgac gaaagacgta aggcttataa
     8161 ttctgacatc acctttggta ctaataatga attcggcttt gactatttgc gtgataatat
     8221 ggcatcttct ccaaaagatt tggtacaaag aatgcacaat tatgcaattg tcgatgaggt
     8281 tgactccgta ttgattgatg atgctcgtac tcctttgatt atatcgggtc ctgttcctaa
     8341 aggcgaggat caattatttg aagaatatcg tcctaaggtt gagaaaattg ttgctgctca
     8401 aaaggcttta gcaacaaagt tacttgctga tgcaaaacgt ttaatggcat cagaaaatga
     8461 gaaagaacgc gaacaaggtg caatttatct gtttcgtacg tataaaggtt tgcctaaaaa
     8521 taatcctctt atcaaatttt tgagcgagcc cggtgtaaaa gctaccatgc ttaaaaccga
     8581 gtctgtgtat atggagcaga atgacagacg tatgcacgag attacagatg atttgtattt
     8641 tgttattaat gagcaacata atagcatcga acttactgat aaaggtatcg atcttataac
     8701 aggtgatact gatgatgcta aattcttcgt attgcctgat attggttctg agttagctga
     8761 tttggaaaag ggaaatatta gcgaagaaga aaaaatatct cgtaaggatg ctttgatgca
     8821 agaatatgca gtaaaatctg aacgtgtaca tactgtaaat caattactta aagcatatac
     8881 cttattcgaa aaggatgatg aatatgtggt tattgataat aaagtaatga ttgttgatga
     8941 gcaaacgggc cgtattatgg aaggtcgccg ttactctgat ggtttgcatc aagctattga
     9001 ggcaaaagag cgtgttactg ttgaagctgc aactcaaact ttcgcaacaa ttacattaca
     9061 gaattatttc agaatgtatc gcaaattggc gggtatgaca ggtactgccg aaacggaagc
     9121 aggcgaattc tgggatatat ataaacttga tgttgttgta attccaacta atagaccgat
     9181 tgttcgtaat gatatgaacg atagggttta taaaacaaaa cgtgtaaaat acaatgctgt
     9241 aattg
//
LOCUS       DAOPID010000038         9167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_9440_length_9167_cov_4.456760, whole genome shotgun sequence.
ACCESSION   DAOPID010000038 DAOPID010000000
VERSION     DAOPID010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 9167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9167
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9440_length_9167_cov_4.456760"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PK641_02405"
     CDS             <1..212
                     /locus_tag="PK641_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55239.1"
                     /translation="GDYLQTILESGPSSFAEVGDFVLIPISGATDIAGTNATIRHLFK
                     PRGVRIRSGSNAKSPMRLTVTYSDL"
     gene            complement(193..606)
                     /locus_tag="PK641_02410"
     CDS             complement(193..606)
                     /locus_tag="PK641_02410"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HPG55240.1"
                     /translation="MESEYLTISEVSEGIYKEKGSKFLGFAHHIESVDEAKSIINKYK
                     KEHHKARHVCFAYRIGNNAEIYRGFDDGEPSGTAGKPILRQLEAAKLTNTIIVVVRYF
                     GGTLLGTGGLINAYKSATSDTISRCKTTYITNQNK"
     gene            725..3370
                     /locus_tag="PK641_02415"
     CDS             725..3370
                     /locus_tag="PK641_02415"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPG55241.1"
                     /translation="MKGLFHFYRFLKLCVLLGAVAMVSVAEAQNLKNNVIPKTDVNYG
                     DLICGSNFSIADNDETTDKTHFTIPEVYVYMPTLASLNLNGIYSEGFSNWSNGGSTTA
                     PKYAIMSNPQLLNASYPSITDGVNRLIVHTPQSQTEIATLTSAGHRAGTPVYFRMTVR
                     ELTGNSGTSKIAVKLNNYGVYQEISGGQTVEISKYIDNAANIASDEIELIIGKDNYNG
                     GDNFVFAISDVVVMGELGGAIASSVSLGEDNRYGVDERISVNLLPMYQGKYIQWQISS
                     NGTDWSNMDGYNAAEFDYSPGLGATFFRALVSESALDPVLVTSNTLMVSRFLACSPQG
                     QYHVLFEDNFGKLASETARECRPDEVSNYTCKGTENTNVEDGDYAVVANPRYAGQDNR
                     PIQVEADYWFRSIYDHTQGGAVGGEYGGMLLINGGSQLLVYAKTFTNPCNNTRVNFSA
                     WFANAGWKRNRGESHDMDVVIVVLDENGIEIPGAEIRVTGTKEEGWKYGVVSFDTGTN
                     SQFTIQIKNSGTGGGYDTLIDDVVVSTCSPDASLFAVDVVEDPENTVAVECGEEVTLE
                     VDRTQIEQIFTSGAYYLWLTKDEGSEYAIDAANSGFDKYSCVFTVNGLSYHKVLIAES
                     ELSATDYFNGIVSPDICEIVAETNEETLLCDVVSISYTRSCNVISLTAESDDNVTWQR
                     SVDGQTWVDMTETDKNITVNITEDSYFKASNEVSKDSTDIIPVQSIKLYANDLVNETI
                     SLGNSVTLETEVEGMTVSTYEWYKNDQLQSAQTGATYNVADLYNKAIFKVEGNDCVSD
                     ELTINVEWPTAFMPYDNGTNKDFARGFTIQIHDRYGNKVAEGSDGWDGKCNGKMSMPG
                     VYYYVVTLPDGDVKKGTIELVKKNK"
     gene            3391..4704
                     /locus_tag="PK641_02420"
     CDS             3391..4704
                     /locus_tag="PK641_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPG55242.1"
                     /translation="MKKIMMPLALLACSLLAFGAIDTKFQEGYELQSLEINTAQREHS
                     MSLFEANTMMFLKGDSVYTAEIDTAATGLSKLVGSKELTNLGINGTVAYDKNKKKIYF
                     ALQESEKVAWLYESTISDGKYTSPKRLIIEGLEKTRGNNGFITNAGWSYAINPKLIMT
                     NPTIAKDGNRIYFTSADIDGGKGGSDIWYIEPKDDNMWKYPVNAGAEINTDANEDYAF
                     VENDEIMYFASNRDTIEFNVFKSLANGDSWAAAEKMEKPYNSEVADYNLIVKDGTPFL
                     ISSRNTGNGDDIYAFVKLPEPEPVVEPEPEPEPEPVVEPEPIVITFPWTSFLFDFDKS
                     NLTDRFIREIDAMVEAMKLMPEGTTYVVEGHTDQRGSFEYNDALSEKRANEVKAKLVE
                     RGVDEKILIIKGYGERKPIFENPKNEEEFYQNRRVVVDTFKPENK"
     gene            4710..5654
                     /locus_tag="PK641_02425"
     CDS             4710..5654
                     /locus_tag="PK641_02425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HPG55243.1"
                     /translation="MKKNFKLLVLFLLVGTSLFAQGDFKMSEQMFSRINQNPSAVGNN
                     EKIQLFTATRLQWVGLGLDQSPTSALLNAHYYLDKINSGFGISFVYDELGLSNQTINA
                     KLVYAYNIDLAENWLLSLGLSAGVINKRFDPNRHVWKDPTDPNRPTEVLATTDFDLDF
                     GFELSYKYLLIGASVTHLPNVAKEVSTTTAPSHINAYVRGNIYLPYKFNLIPAFAYTN
                     IGALNGFKTEVSNEHLLDYSITAMYNNMCWFGLGYRSGVAGTYSSDIMSFMIGYEWHW
                     LRIGYTYDLSVGKLMSLSSSTHEIMLSFIIPTKKPVVW"
     gene            5741..6205
                     /locus_tag="PK641_02430"
     CDS             5741..6205
                     /locus_tag="PK641_02430"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPG55244.1"
                     /translation="MKKYAFIVLGLLLATTAFSQKYYQSMYEPADNDKTTQTEMKQSK
                     EVNIHPINPATDKVPESQDKISESTDIYTKIDGGKVTAIYHVVVGSFQSRINAENLCS
                     TLKNVGKNSFVAQNQQGMFRVFYYSTNAESEVRQALTEARTQYPDAWMLKLK"
     gene            6205..7176
                     /locus_tag="PK641_02435"
     CDS             6205..7176
                     /locus_tag="PK641_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55245.1"
                     /translation="MKCSKILIFIFLIVSVFTSCKDYTRFYKDVSKESVNIKIERFDI
                     DLLNSDTVALRAKYGQFFDLYTYRILGLNNGSQLSMFLNDTSIMRLYSDAEKTYKNVG
                     DIEKELTTAFRYYKCYFPENQIPRVLFHVSGFNQAIVTTDSVVSASIDEYLGSDYPMY
                     QQIVYQYELPFMSKDKLPIDIMYGWLLADYDEDTDSDRLLERMLNQGKLMYLLQVFFP
                     EREPYEFLSYTEEKYEWCEKYEKEVWGYILDNKELFSSDWRTITRYMQPAPFTSGLAS
                     DSPGRIGVFIGWRIISSYMDKQKDLTLKDLMLENDAQKILQLSAYRP"
     gene            7232..8339
                     /gene="prfB"
                     /locus_tag="PK641_02440"
     CDS             join(7232..7294,7296..8339)
                     /gene="prfB"
                     /locus_tag="PK641_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830350.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HPG55246.1"
                     /translation="MITIEQLKEIQERELALRGYLDIDEKLIQIEEEELRTQVPGFWD
                     NIKEAEAQMKKVKDLKRWVDGYNEVKKATEELQLAFDFYKESIVSEEEVDSAYTHVFT
                     LLESMEMKNMLSSEEDHLGAVLKIVSGAGGTESQDWASMLLRMYQRYSERNGFKWIIS
                     NYQDGDEAGIKTATLEIEGDMAYGYLKSENGVHRLVRVSPYNAQGKRMTSFASVFVIP
                     LVDDTIEIEVSPALLTWETFRSGGAGGQNVNKVESGVRLRYQYKDPYSGVEEEILIEN
                     TETRDQPKNKENALRLLRSQLYDRELQHRMAEKAKVEAGKKKIEWGSQIRSYVFDDRR
                     VKDHRTNYQTSDVQGVMDGKIEEFIKAYLMEFDS"
     gene            8380..>9167
                     /locus_tag="PK641_02445"
     CDS             8380..>9167
                     /locus_tag="PK641_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HPG55247.1"
                     /translation="MNQELNDQEQFRRQSLETLRSIGINPYPAAEFPTNAFSTEVKEK
                     FTDDGEKYEVVMAGRLMSRRIMGKASFAELKDSKGRIQLYISRDEICKGEDKELYNTI
                     FKKLLDIGDFIGVKGYVFRTQTGEISVHVSELIVLSKSLRPLPIVKEKDGVVYDAFSD
                     PESRYRQRYVDLVVNEEVKDIFVKRTKVYSSMREYFNSKGYLEVETPILQSIPGGASA
                     RPFITHHNALDIPLYLRIADELYLKRLIVGGFEGVYEFSKTTYD"
BASE COUNT         3083 a         1334 c         1845 g         2905 t
ORIGIN      
        1 ttggagacta tttacaaact atattggaat caggtccgtc atcttttgct gaagtagggg
       61 attttgtctt gattcctatt tcaggagcaa cagatattgc aggtacaaat gctaccataa
      121 gacacttatt taaacctcgt ggcgtacgca ttcgcagtgg ttcaaatgca aaatcgccaa
      181 tgcgtcttac tgttacttat tctgatttgt aatataagta gtcttacatc tacttatggt
      241 atctgaagtt gcgcttttgt aagcgtttat taagcctccc gttcctaata aggttccacc
      301 aaaatatcta acaacaacga ttatagtgtt agtaagcttt gctgcttcta attgtcttaa
      361 tataggttta cctgcagtcc cggaaggttc tccgtcatca aatcctctgt atatctctgc
      421 attgtttcca atcctatacg caaagcatac atgtcgggct ttatggtgct cctttttgta
      481 tttatttatg attgattttg cttcatctac agattctatg tgatgtgcaa atcctaaaaa
      541 tttgctacct ttttccttgt atattccttc agatacctct gaaatcgtta aatattcact
      601 ttccattctg caaaaataca actaattgta aaagaaatgt gttaaaatgt gcttgaataa
      661 taattatgtt ttttaacttt gcgccttata aggtgcagac ctttaaaaac ttaaaacata
      721 taatatgaaa ggattattcc atttttaccg ttttcttaag ctatgtgtgc ttttaggtgc
      781 cgtggctatg gtttctgtag ctgaagctca aaatcttaaa aacaatgtca ttcccaaaac
      841 agacgtcaat tacggcgatt tgatttgtgg tagtaatttt tctattgctg ataatgatga
      901 aactacagac aaaactcatt tcacgattcc tgaagtttat gtgtatatgc caactttggc
      961 tagtttaaat ttaaacggca tctattcaga aggtttctct aattggtcaa atggtggatc
     1021 gacaactgca cctaagtatg cgattatgag taacccacaa ttattgaatg catcatatcc
     1081 ttcaattact gacggcgtta atcgtttgat tgttcatacg cctcaaagtc aaacagaaat
     1141 tgctacatta acatcagccg gtcatagggc aggaaccccg gtatatttta gaatgactgt
     1201 tagagaattg accggtaatt caggaacatc taaaatagca gtaaaattaa ataattatgg
     1261 tgtttatcaa gaaattagtg gtggtcaaac tgttgagatc tctaaatata ttgataatgc
     1321 agctaatatt gcttctgatg aaatcgaatt aataataggc aaggataact ataatggagg
     1381 agataatttt gtattcgcaa tttcagatgt agttgtaatg ggcgaacttg gaggtgctat
     1441 tgcatcatca gtatcacttg gtgaagataa tagatatggc gtagacgaaa gaatctcggt
     1501 aaatctactg cctatgtatc aaggaaaata tatacaatgg caaatatctt ctaatggtac
     1561 tgattggtca aatatggatg gatataatgc agccgaattt gattatagtc ccggattagg
     1621 tgctacattt ttccgtgcat tagttagtga atcagccttg gatcccgtac ttgtaacctc
     1681 aaatacattg atggtttcac gtttcttagc atgttctccg caaggacaat atcacgttct
     1741 ttttgaagac aatttcggaa aattggcaag tgagacagct cgtgagtgtc gtcctgatga
     1801 ggtctcaaat tatacttgta agggcaccga aaatacgaat gtagaagatg gtgattatgc
     1861 agttgtggct aatcctcgtt atgcaggtca agataataga ccaattcaag ttgaagccga
     1921 ttattggttc agaagtattt atgatcatac acaaggtgga gctgttggtg gagaatatgg
     1981 aggtatgttg ttgattaatg gaggttctca attattggtt tatgcaaaaa catttacaaa
     2041 cccatgtaat aatactcgcg ttaacttttc cgcatggttt gcaaatgcag gctggaagag
     2101 aaatcgtggc gaatcgcacg atatggatgt agttattgtc gttcttgacg aaaatggaat
     2161 tgaaattccc ggagctgaaa ttcgcgtaac aggaacaaaa gaagagggat ggaaatatgg
     2221 tgtagtatca tttgatacag gtacaaattc tcagtttaca atacagatta agaattcagg
     2281 aacaggtggt ggatacgaca ctttgattga tgatgttgtt gtttctacat gttcacctga
     2341 tgcatcttta ttcgccgttg atgttgtaga agacccggag aataccgttg ccgtagaatg
     2401 cggtgaagaa gtcacattgg aagttgatag aacacaaatt gagcaaatat tcacttcagg
     2461 tgcatattat ttgtggttaa caaaagatga aggttctgaa tatgcaattg atgcagccaa
     2521 ttcaggtttc gataaatatt catgtgtttt tactgttaat ggtttaagct accataaggt
     2581 attaattgct gaaagtgaat taagtgcaac cgattatttt aatggaattg taagtcctga
     2641 tatatgtgaa atagtagctg aaacgaatga agaaacttta ctttgtgatg tagtatcaat
     2701 atcatataca cgttcttgta atgttatatc gttaacagct gaatcagatg ataatgtaac
     2761 ttggcaaaga tctgttgatg gtcaaacttg ggtcgatatg actgaaacag ataaaaatat
     2821 tactgttaat ataacagaag attcatattt taaagctagc aacgaagtta gtaaagatag
     2881 tactgatatc atacctgtac aatcaataaa attgtatgct aatgatttgg taaatgaaac
     2941 aatttctttg ggaaatagtg ttacattgga gacagaagta gaaggaatga cagtgagtac
     3001 atatgaatgg tataaaaatg atcaacttca atctgcccaa acgggagcta cttataatgt
     3061 agctgattta tataataaag caatatttaa agttgaagga aatgattgcg tatctgatga
     3121 gttgacaatc aatgtagaat ggccaacagc atttatgcct tatgataatg gtacaaataa
     3181 ggattttgct cgtggcttta caatacagat acacgacaga tacggaaata aagttgcgga
     3241 aggttctgac ggctgggatg gtaaatgtaa tggaaaaatg tctatgccgg gtgtatacta
     3301 ttatgttgta acattacctg atggagatgt taaaaaaggc acaattgaat tggttaagaa
     3361 aaacaagtaa taactttaat acataataaa atgaaaaaaa tcatgatgcc attagctttg
     3421 ttggcttgtt cgttgttagc attcggagct atcgacacga aattccaaga aggctatgaa
     3481 ttgcagtcgc tggaaataaa tactgctcag cgcgaacata gtatgtcttt gtttgaggcc
     3541 aatacgatga tgttcttaaa aggagactcg gtatacactg cagaaattga tactgctgct
     3601 accggtttgt ctaagcttgt agggtctaaa gaacttacaa atttaggaat aaatggaacc
     3661 gttgcatacg ataaaaacaa aaagaaaata tactttgcat tgcaagaatc tgaaaaagta
     3721 gcatggttat atgaatctac aatttctgat ggtaaatata ctagtccaaa acgtttaatt
     3781 attgaaggat tggaaaaaac tcgcggaaac aatggattta taacaaatgc aggttggagt
     3841 tatgcaatta atccgaaact tatcatgaca aatccaacta tagctaaaga cggcaatcgt
     3901 atttatttta ctagtgctga tatagatgga ggtaaaggag gttcagatat ttggtatatc
     3961 gaacctaaag atgataatat gtggaaatat cctgttaacg caggtgctga aataaatact
     4021 gatgcaaatg aggattatgc attcgtagag aatgatgaaa ttatgtattt tgcaagtaat
     4081 cgcgatacaa tagaatttaa tgtttttaaa tcactagcca atggtgattc gtgggctgct
     4141 gctgaaaaaa tggaaaaacc atataatagt gaagttgctg actataattt gattgttaaa
     4201 gatggtactc cattcttgat atcatctaga aacacaggta atggcgatga tatttatgct
     4261 tttgtaaaac ttccggaacc ggaacctgtt gttgagcctg aacccgaacc cgaaccggaa
     4321 cctgttgttg aaccggaacc tatcgttata actttccctt ggacatcctt cttgtttgat
     4381 tttgataaat caaatttgac agacagattc atacgcgaaa ttgatgcaat ggttgaagct
     4441 atgaagttga tgccagaagg aacaacttat gttgtagaag ggcatacaga tcaacgtggt
     4501 agttttgaat ataatgatgc attgtcagaa aaacgtgcta acgaggtaaa agcaaaatta
     4561 gtagaacgcg gtgttgatga gaagatactt ataattaaag gttatggtga acgcaaacca
     4621 atctttgaga atcctaagaa tgaagaagag ttctatcaaa accgtcgtgt agtagttgat
     4681 acatttaaac ctgaaaataa ataaaagcca tgaaaaagaa ctttaaatta ttagtgttgt
     4741 ttctgctcgt gggcacaagt ttgtttgctc agggcgattt taaaatgtca gaacaaatgt
     4801 tctctcgtat taaccaaaac ccttctgctg ttggtaataa tgagaaaatc caattgttta
     4861 ctgcaacaag attacagtgg gtaggtttgg gtttggatca atctccaact tcagctttgt
     4921 taaacgcaca ttattatttg gataaaatca attcgggttt cggtataagt tttgtttatg
     4981 acgaacttgg tttgtctaat caaacaatca atgcaaaact agtttatgca tacaatattg
     5041 acttagctga gaattggctt ttgtcattgg gtttgtccgc aggtgttata aataaacgtt
     5101 ttgatccaaa tagacatgta tggaaagatc ctacagatcc aaatcgtcca acagaagtgt
     5161 tggctactac cgattttgat ttagatttcg gttttgagtt gtcatacaaa tatttgttga
     5221 taggtgcatc tgtaacacac ttgccaaatg ttgcaaaaga agtttcaact acgacagcac
     5281 catctcatat taatgcttat gttagaggta atatttattt accatataaa tttaatttga
     5341 ttcctgcttt cgcttacaca aatatcggtg cattgaacgg atttaaaaca gaagtatcaa
     5401 acgagcattt attggattac agcataaccg caatgtacaa taacatgtgt tggtttggat
     5461 taggttatcg ttcaggtgta gctggtacat attcaagcga tattatgtca tttatgatag
     5521 gatatgaatg gcattggttg cgtattggtt atacatatga cttgagtgtt ggaaaactta
     5581 tgagtttgtc ttcaagcaca cacgaaatca tgttatcgtt tataatacca acaaagaaac
     5641 cggttgtttg gtagtttgaa taaattctaa aaaatagagg cttggtgtac taagcctcta
     5701 ttttttttgt atttttgttt ttattaaaaa tatttgaaat atgaagaaat atgcatttat
     5761 agttttagga ctattattag ctactacagc tttttctcaa aaatattatc agtcgatgta
     5821 tgagccggca gataatgata aaacaacgca aactgaaatg aagcagtcaa aagaagtaaa
     5881 tattcatccc ataaatcctg ctactgataa agtaccagaa tcgcaagata agatatcgga
     5941 gtctacagat atttatacaa agattgacgg aggtaaggtt actgctatat accatgttgt
     6001 agttggcagc tttcaaagta gaattaatgc agaaaattta tgtagtacat tgaaaaatgt
     6061 tggtaaaaat tcttttgtgg cccaaaatca acaaggaatg tttagggtgt tttattattc
     6121 tacaaatgct gagtctgaag tgcgacaagc actaactgaa gccagaacac aatatccgga
     6181 tgcatggatg ctaaaactta aataatgaaa tgctctaaga tattaatatt tatttttctt
     6241 attgtatcgg tttttacttc atgtaaagac tatactcgtt tttataagga tgtttcaaaa
     6301 gaatcagtca atatcaaaat agaacgtttt gatattgatt tgttgaatag tgatacagtt
     6361 gcattaagag caaaatacgg gcagtttttt gatctgtata catatcgtat cttaggtcta
     6421 aacaatggtt cacaattgtc tatgttttta aatgatactt cgattatgcg tttgtattca
     6481 gatgcagaaa aaacatataa aaacgtaggt gatatagaaa aagaactaac tactgctttc
     6541 cgttattata aatgttattt tcctgaaaat caaattcctc gtgtgctttt tcacgtttca
     6601 ggctttaatc aagccatagt aactacagat tctgttgtat cagcaagtat tgatgagtat
     6661 ttagggtctg attatcctat gtaccaacaa attgtgtacc aatatgagtt gccgtttatg
     6721 tctaaagata aattaccaat agacataatg tatggttggc ttttggcaga ttatgatgaa
     6781 gatactgata gtgacaggct attggagaga atgttaaatc aaggtaaatt aatgtatttg
     6841 ttgcaggtgt tctttccgga acgtgagcct tacgagtttt tatcatatac tgaagaaaaa
     6901 tatgagtggt gtgaaaaata cgaaaaggaa gtgtggggct atattttaga taataaagaa
     6961 ttattttcat ctgattggcg aacaataact cgttatatgc aaccggcacc atttacatca
     7021 ggccttgcat cagattctcc cggaaggatt ggagtgttta taggatggcg cattattagt
     7081 tcttatatgg ataaacaaaa agatctaaca ttaaaagatt taatgttaga aaatgatgct
     7141 caaaaaattc tgcaattgtc agcttatcgt ccataaaaaa acggcttaat atagttattt
     7201 ttattacctt tgcacctcaa atttatatag gatgattacc attgaacaat taaaagagat
     7261 acaagaacgc gagcttgcgt tgagggggta tctttgacat cgatgaaaag cttattcaaa
     7321 tagaagagga agaacttaga actcaagtac ccggcttttg ggataatatt aaagaagccg
     7381 aagctcaaat gaaaaaggtt aaagacctaa aacgttgggt agatggttac aatgaggtta
     7441 aaaaagcgac agaagagctt caattagcat ttgattttta taaagaaagt attgtttctg
     7501 aagaagaagt agactctgca tatactcatg tctttacttt acttgaaagt atggagatga
     7561 aaaacatgtt atcttctgaa gaagatcatt taggagctgt tttaaaaatt gtatccggtg
     7621 ccggtggaac agaaagtcaa gattgggcat cgatgctgct tagaatgtat cagagatatt
     7681 ctgagagaaa tggctttaaa tggataatta gtaattatca agatggcgat gaagcaggaa
     7741 ttaaaactgc aactctcgaa attgaaggcg atatggctta cggctatttg aaaagcgaaa
     7801 atggagtaca ccgtttggtt agagtatcgc cgtataatgc tcaaggtaag cgtatgactt
     7861 catttgcatc agtatttgta attccattgg tagatgatac gatagagatt gaagttagtc
     7921 ctgcattgtt aacatgggaa acctttagaa gtggtggcgc aggaggtcag aatgtcaata
     7981 aagtagagtc cggtgttaga ttgcgttatc agtacaaaga tccttattct ggtgttgaag
     8041 aagaaattct tattgaaaac acagaaacca gagatcaacc gaaaaataaa gaaaatgctt
     8101 tacgtttgtt gagatctcaa ttatatgata gagaattaca acatcgaatg gcagaaaaag
     8161 ctaaggttga agccggtaaa aagaaaatag agtggggatc ccagatccgt agttacgtgt
     8221 ttgatgacag aagggttaaa gaccatcgta caaattatca gacatctgat gttcaaggtg
     8281 ttatggatgg taaaattgaa gaattcatca aggcatactt gatggaattc gatagctgat
     8341 taaattaata tctgattata tagaaaattt tacaaaaaaa tgaatcaaga attaaacgac
     8401 caggagcaat ttagacgtca aagtctggag acacttagga gtatcggaat taatccgtat
     8461 cccgctgcag aatttcctac caatgcattt tctacagaag tgaaggagaa atttacagat
     8521 gatggcgaaa aatatgaagt cgtaatggca ggacgtttga tgagtcgccg tataatgggc
     8581 aaggcatcat tcgctgaact taaagattct aaaggccgta ttcaattata tatttcgcgt
     8641 gatgaaatat gtaaggggga agataaagaa ctttataata ccatatttaa aaaattgctt
     8701 gatataggcg attttatagg agtaaaagga tatgtattta gaacgcaaac aggagaaatt
     8761 tctgttcacg tttcagaatt aattgtattg tctaaatcac ttcgtccgct tccaattgta
     8821 aaagaaaaag acggcgtcgt ttatgatgct ttttcagatc ccgaatctag atatcgtcaa
     8881 agatatgttg atttggttgt aaatgaagaa gttaaagata tatttgtaaa acgtaccaag
     8941 gtttactctt caatgcgtga atactttaat agtaaaggtt atttggaagt tgaaactcca
     9001 atattacaat caattcccgg aggagcatca gccagaccgt ttattactca tcataatgcc
     9061 ttagatattc cattatattt acgaattgct gacgaattgt atttgaaacg tcttatcgta
     9121 ggtggttttg aaggagtata cgaattctcc aaaaccacct acgataa
//
LOCUS       DAOPID010000039         9101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_9541_length_9101_cov_4.772828, whole genome shotgun sequence.
ACCESSION   DAOPID010000039 DAOPID010000000
VERSION     DAOPID010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 9101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9101
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9541_length_9101_cov_4.772828"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PK641_02450"
     CDS             <1..373
                     /locus_tag="PK641_02450"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPG55248.1"
                     /translation="ELKSLRGIGSGFARRIVKFRDKLGGFYCKEQLLEVYGLDENIFN
                     LIKDGVLVDVSTIKKMKVNELSIKELKNHPYISYYQALAIVNARDANTDKRLNSADEI
                     AISDDITADDIERITHYISFE"
     gene            complement(377..2011)
                     /locus_tag="PK641_02455"
     CDS             complement(377..2011)
                     /locus_tag="PK641_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPG55249.1"
                     /translation="MKRILQIILCLFIGITALNAEPNPKAFLQIVNSLEKGEKKAIEL
                     ITKQIKKDKKEAQWYWFADDYYESEDNTDARIGILKQALSVKKLSQIEETQLRLAMAY
                     FDAGNYKSADSILAVLKQNKKVTKLRRNCIIADSLKRNPIEVIVTDMGDSINTPYDNI
                     WPFASTDKEHFLSTVVIGKKNSTPDQNIIQEDIFVSTKKNGKWQYTEVLNGPMRTNEN
                     EGAQSISADGNYIFYVACNRRENIGSCDIYYIIKNKNTWSKPINAGSPLNTRDWESTP
                     CISAGNSVIYFSSNRPGGYGGKDIWESTISKTSNGQLIFSEPKNLGPKINTSEDEISP
                     YIDPYGKYLYFSSTGHGGMGRFDVFYTQKDKNAEWTEAKNIGYPINTHKDEFGFTLDV
                     DGQTAYLSCNGYEKFSANKRIFSIRLPQSLQINRPLENKGDTIILENIYFDVDMALLK
                     PESAPSLGNLLDFLIVHPNYNVEISGHTDNSGTREHNQSLSMARAKAVVEYLTRHHIS
                     PSRLTYVGYGMDKPIASNNTDEGRAKNRRIEIRIKR"
     gene            complement(2008..2739)
                     /locus_tag="PK641_02460"
     CDS             complement(2008..2739)
                     /locus_tag="PK641_02460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPG55250.1"
                     /translation="MNPFFKAFWLLYLIFIYLPIGIIATILCAIITIVMCAVGDHKVW
                     GYYPGKWWARFICLLTFCRVEVVGSENLKKGQSYVFAANHASIYDVFLIYGYIGVSFK
                     WIMKKELQTVPFIGKACMSAGHIYVNRDGGKQALQSIQRAKEILSEGVSVVVFPEGFR
                     TKNGEVGRFKRGAFEISKDMQLPIVPISLSGCYQVMKVGSSYPVPGKLKLRIHPPVDF
                     KPQNHEEELQLIENIRNIIISGIES"
     gene            2812..4269
                     /gene="rpoN"
                     /locus_tag="PK641_02465"
     CDS             2812..4269
                     /gene="rpoN"
                     /locus_tag="PK641_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HPG55251.1"
                     /translation="MALKQQMKQQMRQNLSPQQLQVIKLLEYSVLELEEKIKHELEEN
                     PALEEGGEIEEHDDLQLDREDNIESADENIDSELDDYLPEDDIADYKIQSDNYSPDDN
                     KQSDFSYSDGITFIEYLSGQLHLMQLSDRQKKLGDYIIGNIDEEGYMRREVAQIVDDI
                     NVHTAWNVTETEIFEALSNVQSLEPAGVGARYLEECLLLQLQRKPLTESLDNAMRIVE
                     ECFDEFGQKHYDRICKELNITDEQLKAAADEILKLNPKPGNAFIEAALVRSADAITPD
                     FIYDCESDTLSLNNRNIPDLHVSREYRQLFEDYTGNPKNKTAAMKDAVNFAKQKLDSA
                     KSFIDAIKQRDDTLLRVMRSIIQYQKKYFCEGDESLLKPMTMKEIADKSDCDVSTVSR
                     VSGSKYIQTDFGLFSLKHFFTEGLMTDSGEEISTREIKRILQTCIEEEDKSRPLADEK
                     LCDILQKKGFLIARRTVAKYREQLGLPVARLRKKI"
     gene            4266..5216
                     /locus_tag="PK641_02470"
     CDS             4266..5216
                     /locus_tag="PK641_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPG55252.1"
                     /translation="MKIKVLITQALFPESYSSILNRYDCIMPKENSFSKAEIMNKITD
                     CDALLSMFNFKIDKEIIDAGTKLRIISNFGVGFNNINVEYASERDIVVTNTPDVVIEP
                     TAELAFGMMLDLVRQISYADRRIRKQSVKWGVLENLSHSLNGKLLGIIGFGNVGQAIA
                     RRAVASGMKIVYNSRNRVSADIEQKYDAKWLNLDGLLSVSDVVSVNTPYTFETHHLIN
                     LERLQMMKYTAYLVNTSRGAVVDEKALIYALQNGIIAGAALDVYEQEPTIPSELVELD
                     NVVLSPHAGTATRDVRNLMASAACQNIINFFEGGSITRVY"
     gene            complement(5307..6020)
                     /locus_tag="PK641_02475"
     CDS             complement(5307..6020)
                     /locus_tag="PK641_02475"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HPG55253.1"
                     /translation="MENKNFAIIIASACIGVCIVISSIIFAFSISSLSENQRTVQVKG
                     LSEREVAANAVIWPIVYKDAGNDLTSLYGSIDSKNLIIKNFLIENGISESEISIAAPN
                     VTDRSAEMYGPEKAAFRFYASAILTVSTDKVDLVRSLITKISSIGKNNISFSSDEYQN
                     QIEYKYTKLNDIKNEMVEEATKNARATAQKFADDSESSLGKIKTASQGQFSINNRDNY
                     TPYIKIVRVVSSIEYFIKD"
     gene            complement(6079..7734)
                     /locus_tag="PK641_02480"
     CDS             complement(6079..7734)
                     /locus_tag="PK641_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPG55254.1"
                     /translation="MEPQLSDRTLGQWVEHWAEVSPDKECIVYSDRDLRFTWKQLNER
                     VDNLAKGMMAIGIEKGSHVGLWATNVPDWLTFLYACAKIGAVCVTVNTNYKQNELEYL
                     CENSDMNALCIIDGTWDCDYVDMTYKMLPELKTCQRGHLHSERFPNMKNVIYIGQEKH
                     RGMYNTAEILLLGSSISDKKLNEVKASVKCHDVVNMQYTSGTTGFPKGVMLTHHNIAN
                     NGYFTGENMKFTQDDKLCVCVPLFHCFGVVLATMNCLTHGCTEVMVEKFDPLLVLASV
                     HRERCTALYGVPTMFIAELNHPMFDMFDLTCLRTGIMAGSLCPIELMRKVCDKMHMTI
                     TSVYGLTESSPGMTQTTVNDSFEQRCTTVGRNYPLVEVEVIDPETGEICPPEVQGEMC
                     CRGYNVMKGYYKNPEATAETIDKNGFLHSGDLGIKDKDGYYRITGRIKDMIIRGGENI
                     YPREIEEFLYHLEGVSDVQVAAIPSKKYGEEIGAFIILKEGAKLEAEDVKDFCKDKIS
                     RYKIPKYIFFVDSFPLTGSGKIQKFKLKDIALKICAEQGVEII"
     gene            complement(7744..8319)
                     /locus_tag="PK641_02485"
     CDS             complement(7744..8319)
                     /locus_tag="PK641_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HPG55255.1"
                     /translation="MAVRKLVGEKIKGIRESKKISREDLAANSGLEIGLINAIEENSN
                     IPSLSPLIKIARALGVRLGTFLDDSDLIGPEVCRKGAAEKSMSTSNTKSRSNSHLDFT
                     SLASHKAGRSMEPFVITIEPADTKQLLLSSHEGEEFIYVMEGVVKIIYGKDEYLLNEG
                     DSIYYDSIVEHLVCAGNNQPSKILAVVYSPF"
     gene            complement(8502..8615)
                     /locus_tag="PK641_02490"
                     /pseudo
     CDS             complement(8502..8615)
                     /locus_tag="PK641_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            8748..>9101
                     /locus_tag="PK641_02495"
     CDS             8748..>9101
                     /locus_tag="PK641_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine carbamoyltransferase"
                     /protein_id="HPG55256.1"
                     /translation="MRTYTSVANLGNLQTALKEAFEVKSNPYKWDTLGKNKTLLMVFF
                     NSSLRTRLSTQKAVMNLGMNTIVLDVNAGAWKLETERGVIMDGDKPEHLLEAIPVMGS
                     YCDLIGVRSFAGLENK"
BASE COUNT         2824 a         1604 c         1634 g         3039 t
ORIGIN      
        1 tgaactcaag tctttacgcg gaataggtag cggatttgct cgtagaatag taaaattcag
       61 agataagtta ggcggtttct attgtaaaga gcaattgcta gaggtttatg gattagatga
      121 aaatattttt aatcttatta aagatggggt tttggttgat gtatcgacaa ttaaaaagat
      181 gaaagtgaat gaattgagta taaaagagct gaaaaatcat ccatatatat catactatca
      241 agcacttgca atagtaaacg cgcgtgatgc aaatacagat aaaaggctaa attctgccga
      301 tgaaatagct atttctgatg atattaccgc tgatgatatt gagcgcatta cacactacat
      361 atcattcgaa tagactttaa cgtttgattc ttatctcgat tcgtctgttt tttgcacgtc
      421 cttcatcagt gttgtttgag gctattggtt tgtccatacc atatccaaca tacgtgagtc
      481 tacttggcga gatatgatgt cttgtcaggt attctacaac agcctttgct cttgccatgg
      541 aaaggctttg attgtgttcg cgtgtacctg aattatctgt atgacccgaa atctcaacat
      601 tgtagttagg atgtacaata aggaaatcaa gcaaatttcc gaggcttggt gcagactcag
      661 gttttagcaa tgccatatca acatcaaaat agatattctc taatattata gtatctcctt
      721 tattctcaag tggacgatta atttgtagtg attgcggtag tctaattgaa aaaatccttt
      781 tgtttgccga aaatttttca tacccattgc atgatagata tgctgtttga ccatctacat
      841 caagagtaaa accgaattcg tctttatgtg tattaatagg ataacctata tttttagctt
      901 ctgtccattc tgcatttttg tctttttgag tataaaaaac atcaaatctg cccattccac
      961 catgacctgt cgaagaaaaa tataagtatt tgccataagg atctatgtat ggagagattt
     1021 catcctcaga agtattaatc ttaggcccaa ggtttttagg ctcgctaaag attagttgac
     1081 cattagatgt tttgctaatt gtactttccc aaatatcttt tcctccgtat cctccgggtc
     1141 ggttactcga aaaataaata acagaattgc cggcagatat acaaggcgtg ctttcccaat
     1201 cacgagtatt taaagggctg cctgcattaa taggcttact ccatgtgttt ttgtttttaa
     1261 taatgtaata gatatcgcaa cttccaatat tttctcttct gttgcaagct acataaaaaa
     1321 tgtagtttcc atcggcagaa atgctttgag ctccttcgtt ttcgttagta cgcatagggc
     1381 cattcaatac ttctgtgtat tgccatttcc cgttcttctt tgtacttaca aaaatatctt
     1441 cctgaataat gttctggtcc ggagtcgaat tcttttttcc aattacaact gtacttaaga
     1501 aatgttcttt atctgtagat gcaaaaggcc aaatattatc atagggagtg ttgatgctat
     1561 ctcccatatc agtaacaatt acttctatag gatttcgttt taatgagtct gctataatgc
     1621 aatttctgcg aagttttgta actttcttat tttgttttaa tacggcaagt atagaatcgg
     1681 ctgatttgta attaccggca tcaaaatatg ccattgccaa tcttaattga gtttcttcga
     1741 tttgcgataa ttttttaact gataaagctt gttttaatat ccctatacga gcatcagtat
     1801 tgtcttccga ttcgtaataa tcatctgcaa accagtacca ttgtgcttct tttttatctt
     1861 tttttatttg ctttgttatg agttcaattg cttttttttc tcctttctca agagaattca
     1921 caatctgtaa aaaagcttta gggtttggtt ctgcatttaa tgcagttatg ccgatgaata
     1981 ggcataaaat tatttgcaga atacgtttca tgattcgatt cctgagataa ttatattgcg
     2041 aatattttct atcaattgta gctcttcttc atggttttga ggtttaaaat ctacaggtgg
     2101 atgtatgcgt agcttaagtt tccccggtac aggatatgaa cttcctacct tcatcacctg
     2161 gtagcatccc gataagctta ttggtactat tggtaattgc atgtccttag atatttcgaa
     2221 cgctcctcgt ttaaaacgtc caacctcacc attttttgtg cggaaaccct ccgggaatac
     2281 aacaaccgaa acaccttcgc ttaatatttc tttggctctt tgaatagatt gtaaagcctg
     2341 ttttccccca tctctgttaa cataaatatg tcctgccgac atacatgctt ttccaataaa
     2401 aggaactgtt tgcaactcct ttttcataat ccatttgaac gatacgccta tatatccgta
     2461 aattaagaat acatcgtata tgcttgcgtg atttgcagca aaaacataag actgaccttt
     2521 tttaagattc tcacttccaa caacttcaac ccgacaaaaa gttagcaaac aaataaaacg
     2581 tgcccaccat ttaccggggt aatatcccca tactttatgg tctccgacag cgcacataac
     2641 aatagtaatt atggcgcaca aaatggttgc aataatgcct attggcagat aaataaaaat
     2701 gagataaagg agccaaaatg ctttaaaaaa cggattcata tttgaaggtt tacatgctgt
     2761 tttgacaaaa gtactgattt ttttgtacat ttgccttcaa atccgaaatt tatggcacta
     2821 aaacaacaaa tgaagcagca aatgcggcaa aatctgtcgc cgcagcaatt gcaggtcatc
     2881 aaattgttgg agtattccgt tctcgaatta gaagaaaaaa tcaaacacga actagaagaa
     2941 aatccggcac ttgaagaagg gggagaaata gaagagcacg atgatctgca attggatagg
     3001 gaagataata tcgaatctgc cgatgaaaat atagattctg aattggatga ttatttgccc
     3061 gaagatgata ttgccgatta taaaatacaa tctgataatt attctcctga tgataacaag
     3121 caaagcgatt tttcgtattc tgatggtata acttttatag aatatttgag tggacaatta
     3181 cacttaatgc agttgagcga tcgtcagaaa aaattaggtg attatataat cgggaatatt
     3241 gatgaagaag gttacatgcg ccgagaagtt gcgcaaatag ttgatgatat taatgttcat
     3301 acagcttgga atgtaactga aacagaaatt tttgaggcct tatcgaatgt acaatcttta
     3361 gaacctgcag gcgttggtgc aagatatctt gaagaatgtt tattgttgca actgcagcga
     3421 aaaccattga ccgagtctct cgataacgct atgcgaattg ttgaagaatg tttcgatgaa
     3481 ttcggacaaa aacactatga taggatttgc aaggaactta atataacgga tgagcaatta
     3541 aaggcagcag ccgatgaaat tttaaaactg aatccaaaac cgggaaatgc ctttattgag
     3601 gctgctttag tccgttcggc tgatgctatt actcctgatt ttatatatga ttgcgagtca
     3661 gatacattat cattaaataa tagaaatata cccgatttgc acgtaagtag agaatacagg
     3721 cagttgtttg aagattatac agggaacccg aaaaacaaaa ctgctgcaat gaaagatgca
     3781 gttaattttg caaagcaaaa attagacagt gccaaatctt ttattgatgc aataaagcaa
     3841 agagatgata ccttgcttag agtgatgcga tcaataatac aatatcaaaa aaagtatttt
     3901 tgcgaaggcg atgagtcatt gcttaaacca atgacaatga aagagatagc agacaaatca
     3961 gattgcgatg tgtctaccgt gtcgagagta tcaggcagta aatatatcca aacagatttt
     4021 ggtttgtttt cgttaaagca tttttttacc gagggtttaa tgaccgatag tggagaagaa
     4081 atttctactc gtgagataaa acgaatcttg cagacttgca ttgaagaaga agacaagtca
     4141 aggcccttgg cggatgaaaa attatgtgac attctgcaaa aaaaaggttt tttaatagca
     4201 aggcgtactg ttgctaaata tcgagagcaa ctgggtttac cggtagcacg tttacgtaaa
     4261 aagatatgaa aataaaagtt ctaataacac aagcattgtt tccggaatct tattcaagta
     4321 ttctgaatcg ttatgattgt ataatgccga aagaaaactc tttttcgaaa gctgagataa
     4381 tgaataaaat taccgattgt gatgctcttt tatcgatgtt taattttaaa attgacaaag
     4441 aaataattga tgcaggtact aaattgcgca taatttcaaa ttttggagta ggttttaata
     4501 atattaatgt tgaatatgct tcagaaagag acatagtagt tacgaatacg ccggatgttg
     4561 ttattgagcc gactgcagag cttgcttttg gcatgatgct ggatctagtt agacagataa
     4621 gttacgctga cagaagaatt cgtaaacaat cggtgaaatg gggagtttta gaaaatcttt
     4681 cgcatagctt aaacgggaag cttcttggaa ttataggttt tggtaatgtg ggtcaggcca
     4741 tagctcgtag ggctgttgca tcaggcatga agattgtgta taatagtaga aatagagtct
     4801 ctgctgatat agaacaaaaa tatgatgcaa agtggctaaa tttggatggc ttattatctg
     4861 tatctgatgt tgtaagtgta aacactccat atacttttga aacccatcat cttattaatt
     4921 tagaacgctt acaaatgatg aaatatactg cgtatcttgt aaatacctcg cgcggtgctg
     4981 tagttgatga aaaagctttg atttatgctt tacagaatgg tataatagcc ggtgctgcct
     5041 tagatgttta tgagcaggaa ccgacgattc catctgaatt agttgaatta gataatgttg
     5101 ttttatctcc gcatgccggc acagcaactc gcgatgtccg caaccttatg gcatcagccg
     5161 catgtcaaaa cattataaac ttttttgaag gtggttcaat tactcgagtt tactaaaact
     5221 gcataaaatt aaatagtgct tatacaaatt agtaaggcgt ttaaaatcaa ttaaacgcct
     5281 tactaatata tcaaatgtta aattatttag tccttaataa aatattctat agaactaact
     5341 actcttacaa tctttatgta tggagtgtag ttatctctat tattaatact gaattgacct
     5401 tgtgatgcag ttttgatttt acctaaagaa ctttcagaat catcggcaaa tttttgcgca
     5461 gtagctcttg cattcttggt tgcttcctca accatttcat tcttaatatc attaagcttt
     5521 gtgtacttgt attcaatttg attttggtat tcgtcagaac tgaatgatat gttgtttttg
     5581 cctatgcttg aaattttagt aatcaaactt cttactaaat caaccttatc tgtagaaaca
     5641 gttaaaattg cacttgcata gaatctaaat gcagcttttt ccgggccata catttcagca
     5701 gagcggtctg ttacatttgg agctgcaata gaaatttctg attctgagat accattctca
     5761 attaaaaagt tctttataat cagattctta ctatctatac taccatacaa tgaagttaga
     5821 tcgttaccgg catctttgta aacgataggc catattacag cgttagctgc tacttcacgt
     5881 tctgacaaac cttttacctg cactgtgcgt tgattttcgc ttaaactgct aatgctgaaa
     5941 gcaaaaataa tggatgaaat tactatgcaa acgccaatac atgcacttgc aatgatgata
     6001 gcaaaattct tattttccat agtcttagtt tttatgttgc tttacaaaga tattgattat
     6061 tgtataatat caaacatatt aaataatttc aacaccttgc tcagcacata tttttagagc
     6121 gatgtcttta agtttgaatt tttggatttt tccgctacct gtaagtggga aagaatcaac
     6181 aaagaaaatg tattttggta ttttgtaacg agaaatctta tccttacaaa aatcttttac
     6241 atcttcggct tcgagtttag caccttcttt aagaattatg aatgcgccga tctcttctcc
     6301 atatttcttt gaagggattg cggctacttg tacgtcgcta actccctcta gatgatataa
     6361 gaattcttca atttctcttg ggtatatgtt ttcacctcca cgaataatca tatccttaat
     6421 acgtccggtg atgcggtaat atccgtcttt gtcttttatg ccaaggtcgc ccgaatgtaa
     6481 aaagccgttt ttatcaattg tttcggcagt agcttccgga ttcttgtagt atcctttcat
     6541 tacattatat cctctacagc acatttcgcc ttgaacttca ggaggacaaa tctcccctgt
     6601 ttccgggtca ataacttcaa cttcaactag aggataatta cgacctactg tagtacaacg
     6661 ttgttcaaag ctatcattaa cggtagtttg agtcataccc ggagaacttt ctgttaatcc
     6721 atatacgctg gtaattgtca tgtgcatttt atcacatact tttcgcatca actctatagg
     6781 acacaaacta cctgccataa taccggtgcg tagacaagtt aaatcaaaca tatcaaacat
     6841 aggatgattc agctctgcta taaacatagt aggaacacca tataatgcag tgcatctctc
     6901 gcgatgtaca gaagctaata ccaaaagtgg gtcaaatttt tcaaccataa cctcagtgca
     6961 tccgtgtgtc aaacaattca ttgttgcaag tacaacaccg aagcagtgaa atagaggaac
     7021 acatacgcac aatttgtcgt cttgtgtaaa tttcatattc tctccggtga aataaccatt
     7081 attggctatg ttgtgatgag ttaacataac tcctttcgga aatcctgttg tacctgatgt
     7141 atattgcata ttaactacat cgtggcattt aaccgaagct tttacttcat ttaatttttt
     7201 atcgcttata gaactaccta aaagtaatat ctcagcagta ttatacattc cgcgatgttt
     7261 ctcctggcca atgtagatta catttttcat gttagggaaa cgttcactat gcaagtgtcc
     7321 gcgttggcat gttttcagct caggtagcat tttgtatgtc atatccacat aatcgcaatc
     7381 ccatgtacca tcaatgatac aaagggcatt catgtcagag ttctcacata aatattctaa
     7441 ctcgttttgt ttgtagttag tgtttaccgt tacacaaaca gcaccgattt ttgcgcaggc
     7501 gtaaagaaaa gtaagccaat caggaacatt agttgcccat agtccaacgt gtgaaccttt
     7561 ttcaatgccg attgccatca tgcctttggc taaattgtca actctttcgt tcaattgttt
     7621 ccaagtaaaa cgaaggtcac gatctgaata aactatgcac tccttatcgg gcgatacttc
     7681 tgcccaatgc tctacccatt gtcctaaggt tctgtctgaa agctgtggct ccataattta
     7741 tttttaaaat ggtgaatata ctacggcaag tatttttgac ggttggttat tacctgcgca
     7801 aactaaatgt tctactattg aatcatagta aatgctatct ccttcgttta gtaaatattc
     7861 gtctttgccg taaataattt ttacaacacc ctccataacg tatataaatt cttctccttc
     7921 gtgtgatgag agtaataatt gttttgtatc ggcaggttct attgtgatta cgaaaggttc
     7981 catgctacgt cctgccttat ggcttgccaa cgaagtaaaa tcaagatgtg agttcgagcg
     8041 actttttgta tttgaagtac tcatactctt ttcagcagct ccttttcggc aaacttcagg
     8101 accaataaga tcactatcgt caaggaaagt acctagtctt acacctagtg cacgagcaat
     8161 ttttataagt ggtgagagcg aaggtatgtt actattctct tctattgcat taattaaacc
     8221 gatttccaat ccgctgtttg cagccaaatc ttcacgcgat attttctttg attcgcgaat
     8281 acctttaatc ttttctccta ccaatttacg tactgccata aaattcaatt ttaaaaaatt
     8341 aggtagcaaa aataagcaat ttttttaaaa gaaaagggct atcaacagtg atttattgtt
     8401 ttatgccttg tttttgttac aatttatttg tttgattaaa aatattgttt ttttcagatg
     8461 taattgattc aagtagcaag tgcgaaatta catttttttc aaagactgcc accacatttc
     8521 ttgtcaaaac gaatatattg gtatatcgtt tctgctgaaa tatatggttc tcctctacgt
     8581 cttaaatagc cgggaatctg ttcaggagac cattcttcat ttgaaggtca aatttgcgat
     8641 ccttacacat tctgtttttt gaaaatcacc ccaatatgta tctatcaata catacaagtg
     8701 gaatatttaa aacaaatttg tatctttgaa ctcgtaaaaa ttactatatg agaacttaca
     8761 caagtgttgc caatctaggc aatttacaga cagctttgaa agaagccttt gaagtaaaat
     8821 caaacccata caaatgggat actttaggga aaaacaaaac cttgttgatg gtgtttttta
     8881 attcgagttt aagaactcgc ctgagtactc agaaggccgt aatgaattta ggaatgaata
     8941 ctatcgtact cgatgtaaac gcaggtgctt ggaaacttga aacagaaaga ggtgttatta
     9001 tggatggtga taaacctgag catttacttg aggcaattcc tgtaatgggt agttattgtg
     9061 atttgatagg agttcgttca tttgcgggtt tagaaaataa a
//
LOCUS       DAOPID010000040         8775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_10036_length_8775_cov_3.925573, whole genome shotgun sequence.
ACCESSION   DAOPID010000040 DAOPID010000000
VERSION     DAOPID010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 8775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8775
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10036_length_8775_cov_3.925573"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..2460)
                     /locus_tag="PK641_02500"
     CDS             complement(163..2460)
                     /locus_tag="PK641_02500"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HPG55257.1"
                     /translation="MVKITKEAALLYHSQGRPGKIEVVPTKPYSTQTDLSLAYSPGVA
                     EPCLEIQSKPETVYDYTDKGNLVAVISNGTAVLGLGDIGAMSGKPVMEGKGLLFKIYA
                     GIDVFDIEVDEKDPDKFIEIVKGIAPTFGGINLEDIKAPECFIIEERLKKELDIPVMH
                     DDQHGTAIISSAGLINALEVAGKKIEEVRIVVNGAGASATSCTKLYESLGARRENILM
                     LDSKGVITSDRNGLTENKKYFATDRRDVHTLEEAIKGADVFLGLSKGNVLTQDMIRSM
                     AANPIVFALANPVPEISYEEAIESRPDVLISTGRSDYPNQINNVIGFPYIFRGALDTR
                     SRAINEEMKLAAVHAIAGLAKKPVPDVVNTIYNVNKLSFGPKYFIPKPVDPRLITDVS
                     IAVAKAAIESGVARKNIDDWEAYKTWLSELMGQGSKLTRQMYDMARHNPQRVVFAEGI
                     HPNMLKAAVQAKAEGLCYPVLLGNDEKIGKLATELELSLDGIEIVNLRHDNEAERRER
                     FAHILAEKHARKGYNFQEANDKMFQRNYFGMMMVETGEADALITGLYTNYADTIKVAK
                     EVIGIRKGYSHFATMHILNSKKGTIFVADTLINRHPDTDTLIDVARLSAETVESFNHT
                     PNMAMLSYSNFGSDEKGSPEQVHEAVAFMQEQYPDLAIDGEMQVNFALNKKLRDEKYP
                     FTRLNGKDVNTLIFPNLSSANSGYKLIQAMSETEVIGPIQMGLNKPIHFTDVECSVRD
                     IVNITAYAVIDALVSKRKAIDQMIK"
     gene            complement(2674..4035)
                     /locus_tag="PK641_02505"
     CDS             complement(2674..4035)
                     /locus_tag="PK641_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018464859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HPG55258.1"
                     /translation="MKKRWIILMAFMVLFAVLGLVLPESASWSSENEPIDYANVAWMI
                     TATIFVLMMTPGLSFFYGGMVRAKNVISTMLQSFMAMGIISIIWVVIGFSLAFGDDIG
                     GFIGNPFTYFMFRNVGAHIPSGNENLSGLGLTIPLALYALFQMKFAIITPSLITGSFA
                     ERVHFSAYLVFMALFCLFVYCPLAHWTWHPDGFLNKMLVKDFAGGIVVHASSGVAALV
                     GALFLGRRKQSTKHVPANIPFVMLGAGMLWLGWFGFNGGSSLAADSVAVKAFLNTNIA
                     SATAMIVWILFDCLRGHKPSAMGAAVGAVVGLVSITPSAGWVSVGQSICIAFITALVC
                     NIAIHWKNKTNVDDALDVFPTHGIGGILGTILTGVFVYETNEISYFHSFLNHLLSVII
                     VIVYTFLVTYALYWITNKMIKMRVSEKNEKVGLDMSQHDELYGVEMLTNSRELAEYEL
                     TQD"
     gene            4337..5638
                     /locus_tag="PK641_02510"
     CDS             4337..5638
                     /locus_tag="PK641_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HPG55259.1"
                     /translation="MIWNETAECMDREEMRKLQSERLRNVVDRVYYNCEPYRKRMQEA
                     GVMPGDIQTIDDIVKLPFTSKKDLRDYYPFGLLSSPMSEIVRVHASSGTTGKPIVVGY
                     TRNDLNIWKEVGARCLTAFGLGKNDVVQVSYGYGLFTGGLGAHGAVETIGGTVIPMSA
                     GNTEKQLLMMHDLGAKALACTPSYALYLAESMEKSEFPRDEFQLKVGIFGAEPWTEQM
                     RQEIEHKMGIKAYDIYGLTEIMGPGVGHECQFQCGTHLCEDHFFPEIIDPETGIPVAP
                     GEKGELVFTTLTKEGIPMIRFRTRDLTHLIYDKCECGRTSVRMAKIMGRCDDMLIIRG
                     VNVFPSQIEAVICETEEFEPHFFITVDRVNNTDVFEIQVEVKEQYYTDDISGMLNLQK
                     KLAHKIQSVVGIQPTIRLVEPRSLERSQGKAKRVLDKRVFK"
     gene            5659..6060
                     /locus_tag="PK641_02515"
     CDS             5659..6060
                     /locus_tag="PK641_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007845457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HPG55260.1"
                     /translation="MATKQLSVFLENKTGRLNDVTKILGQAGVNMTAFSVADNSDFGI
                     LRVIVPEPEKVRTLLKDRGFAVSLTDVIAVNIPNKSGSLDKVLDLLAEAKIYIEYMYA
                     FSEGETASVVIKPDNMVQASEILQKHKIHLL"
     gene            6063..6836
                     /gene="surE"
                     /locus_tag="PK641_02520"
     CDS             6063..6836
                     /gene="surE"
                     /locus_tag="PK641_02520"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HPG55261.1"
                     /translation="MNRPLILATNDDGIDARGIHSLIEAVTPLGDVVVVAPDGPRSAQ
                     SSAITVNTPLRIWKHYEKDGLVMYRCNGTPTDCVKIGLYKVLDRRPDVVVAGINHGSN
                     TSISSIYSGTMGAVIEGCIEGVPSIGFSLCDYDPRADFTEAVRYAAIITQKVLDEGLP
                     KDICLNVNVPPVEHVKGMKVCRQCYGRWKERFVIREDPAGNNYYWLTGDFFDLETDAE
                     DTDEAAMRNSYVSVVPCRVDLTDYDAINKLKSWNYEETR"
     gene            6820..7098
                     /locus_tag="PK641_02525"
     CDS             6820..7098
                     /locus_tag="PK641_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55262.1"
                     /translation="MKKLDNFYLGLVSCLLLPFLLFVVTWNIVSGTPLSEVPIWMHQP
                     VFLQYLIFCSLPDLLILFWGYHTERWNICRGGVLGLTPYLMLLFYLFF"
     gene            7098..8237
                     /gene="lpxB"
                     /locus_tag="PK641_02530"
     CDS             7098..8237
                     /gene="lpxB"
                     /locus_tag="PK641_02530"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006953491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HPG55263.1"
                     /translation="MKYYLIAGEASGDLHGSNLMKSIRVNDPDAEFRYYGGDLMQAQG
                     GSLVRHYRDTAVMGFVRVVMHAKTILKNIQQCKEDIKLYQPDAVILIDYPGFNLQIAR
                     YVKEKLSIPVFYYIPPKIWAWKTSRVKLIRKYIDRVYAIFPFEPGFYAKFACEAVYVG
                     NPTVESVSRAHTCSMADFLKKNSLPDKKIIALLPGSRKQEVGLTISVLNEIDFTQFEG
                     YQCVMAATSAVDMRMYQPAINKGIRIVYDQTYDLLANASIAIVNSGTATLEAALFNVP
                     QIVCYPIRIPGVLYDFGRKYMIKIPYISLVNIIANKQVVKELLAYQFSAKNTISELKK
                     IIQNDEYRNLMMSDYRQISSNLGSSVASDIASHDIISTIKKQSLQ"
     gene            complement(8272..8709)
                     /locus_tag="PK641_02535"
     CDS             complement(8272..8709)
                     /locus_tag="PK641_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55264.1"
                     /translation="MEIQSSSRMDNVTMKNIILSEMLDTHTVRLGVEIKPIEALSLRI
                     GGGYTTPCESFNGSRLYYDNDTRTDFEYYNDNGSYNITAGVGYRIGRHAIDLAYVYQV
                     NMANYYEFSNPSSSMYYISDLVVDPIDLRSVRNQIVLSYGIRF"
BASE COUNT         2793 a         1564 c         1602 g         2816 t
ORIGIN      
        1 acctaatttt aatgttaata attcaggtat atatattgtt cgtatcaatg gaacgagcta
       61 taaagtagct gtatattaat aaaattcaac ctaactgtta aaaataaaaa tccgcaacaa
      121 ttaattttgt cgcggatttt tatttttgta aatcaatatc aattatttta tcatttggtc
      181 tatagctttc ctttttgata ctaaagcgtc aattacggca taagctgtaa tatttacgat
      241 atcgcgtaca gaacattcta catctgtaaa atgtattggt ttgtttaatc ccatttgtat
      301 tggtcctata acttctgttt cgctcattgc ttgtatcagt ttataacctg agttcgcaga
      361 acttaagtta gggaaaataa gagtattaac gtctttgcca tttaatcttg taaacggata
      421 tttttcatcg cgcaattttt tgtttagcgc aaaatttacc tgcatttctc cgtcaatagc
      481 taaatcagga tattgctctt gcataaacgc aacggcttcg tgtacttgtt cggggctgcc
      541 tttctcgtct gaaccgaaat tagaatacga caacatagcc atatttggcg tgtgattaaa
      601 tgattctact gtttcggccg acaatctggc tacgtcaatc aaagtatcgg tatccggatg
      661 cctattaatc aaagtgtcag ctacaaatat cgttcctttt tttgaattta aaatatgcat
      721 tgtagcaaaa tgagaataac cttttcggat gcctataact tcttttgcta cttttattgt
      781 atcagcataa ttagtgtaca aacctgttat aagagcatct gcttcgccgg tttcaaccat
      841 catcattcca aaataattgc gttgaaacat tttatcatta gcttcctgga agttgtatcc
      901 tttgcgtgca tgcttttctg ccaatatgtg tgcaaatctt tctcttcttt ctgcttcgtt
      961 gtcgtgcctt agattaacaa tttctatacc atcaaggctt agttctaatt ctgtcgctaa
     1021 ttttcctatt ttttcgtcat taccaagcaa aacagggtag cataaacctt cggcttttgc
     1081 ttgaactgca gcttttaaca tatttggatg tatgccttct gcaaaaacaa cgcgttgcgg
     1141 gttatgacgc gccatgtcat acatctgacg tgttagctta gacccttgtc ccattagttc
     1201 gctgagccat gttttgtatg cttcccaatc gtcaatattc tttcgcgcaa ctccagattc
     1261 aatagctgct ttggctactg ctattgaaac gtcggttatt aatcgtggat caacaggttt
     1321 tggaataaaa tatttaggcc cgaaagataa tttattaaca ttgtatatag tatttacaac
     1381 atcaggtaca ggctttttag caagacctgc aattgcatgt actgcagcca atttcatctc
     1441 ctcatttata gcgcgcgaac gagtgtcaag tgcacctcta aaaatgtatg ggaatcctat
     1501 tacgttattt atctgattag gataatcaga tcgtccggta ctaattagta cgtcagggcg
     1561 tgattcaata gcttcttcgt atgatatttc cggtaccggg tttgctaaag caaatactat
     1621 aggatttgca gccatacttc gaatcatatc ttgtgtaagt acattcccct ttgataaacc
     1681 taaaaacaca tcggctcctt ttatagcttc ttccaatgta tgaacatcac gtctgtcggt
     1741 agcaaaatac ttcttatttt cggttaatcc atttctgtca gatgtaatca cacctttgct
     1801 atctaacatt aatatatttt cgcggcgcgc tccaagtgat tcatacaatt tggtgcatga
     1861 tgtggctgat gctcctgcac cattaactac aatgcgcacc tcttctattt tcttccctgc
     1921 tacttctaat gcatttatta agcctgccga cgatataatt gctgttccat gttgatcatc
     1981 gtgcatcaca ggtatatcaa gttccttttt taggcgttcc tcaattataa aacattcagg
     2041 agcttttata tcttccaaat taattcctcc aaaagtagga gctatacctt taacaatttc
     2101 aataaatttg tccggatctt tttcgtctac ttcaatgtca aaaacgtcaa ttccggcata
     2161 tatcttaaaa agaagtccct tgccttccat gacaggtttc ccgctcatgg caccaatatc
     2221 gccaagtccc aataccgctg ttccgttcga aataactgcc acaagatttc ctttgtcggt
     2281 atagtcgtat acagtttcag gcttgctttg gatttccaaa catggctctg ctacaccagg
     2341 tgagtatgct aaagaaaggt ctgtttgtgt gctgtatggt tttgttggta ctacctctat
     2401 tttccctggt cttccttgag agtgatatag caaggcagct tctttggtta ttttgaccat
     2461 attgtataaa attattagtt attattaggc ggtgcaaaat taagctttat tttaaagtag
     2521 tattgcatat tgataaaaac ttttttaatc aagttataat aatttatttc ataagatttt
     2581 agtatcataa aatcagtcaa ataatatatg tcatataatc acatggttta attatcggca
     2641 atctttataa tggacaaata tcaattactt agactaatct tgagtaagtt cgtactcggc
     2701 taattctctg ctatttgtaa gcatttcaac gccatataat tcatcatgtt gactcatgtc
     2761 aagacctact ttttcattct tttcagatac tctcatttta atcatcttgt ttgtaatcca
     2821 atataaagcg taagtaacca agaatgtata aacaataaca attataactg acaataagtg
     2881 atttagaaaa ctatgaaaat agctgatctc atttgtttca tatacaaata cgcctgttaa
     2941 tatcgtaccc aaaataccgc ctattccgtg agttggaaat acatcgagcg catcatcaac
     3001 attggttttg tttttccaat ggatagctat attacacaca agagcagtta taaatgctat
     3061 gcaaatgctt tgtccaaccg aaacccatcc ggccgatggt gttattgata caagtcctac
     3121 tacagcgcca acagctgcac ccatagcaga tggtttatgt ccacgcaaac aatcaaataa
     3181 aatccaaacg atcattgcgg tagctgatgc tatattggtg tttagaaaag cttttaccgc
     3241 tacactgtcg gcagccagcg acgagccgcc attaaatccg aaccaaccaa gccataacat
     3301 tcctgcgcct agcataacaa atggtatatt tgccggaacg tgttttgtag attgttttct
     3361 tctgcctaag aacaaggctc ctaccaatgc ggctacacct gatgatgcat gaacaactat
     3421 acctccggca aaatctttta caagcatttt atttaaaaat ccatcaggat gccaggtcca
     3481 gtgtgccaaa ggacaatata cgaacaagca aaataacgcc ataaatacaa gatatgctga
     3541 aaaatgcacg cgttcggcaa aagaaccggt tatcaaagag ggtgttatga tggcaaattt
     3601 catttgaaac agtgcgtata atgcaagagg tattgttaat ccaagtccgg ataagttttc
     3661 gttaccagaa ggaatgtgag ccccaacatt tctaaacatg aaatacgtaa atggatttcc
     3721 aataaatcct cctatatcat caccaaaggc aagactgaat ccgatcacta cccatataat
     3781 actaataatg cccattgcca taaaactttg cagcatagtg gatattacgt tttttgctct
     3841 aaccattcca ccataaaaga aagaaagtcc aggagtcatc attagtacaa aaatagttgc
     3901 tgtgatcatc caagcgacat tagcgtagtc tattggttca ttttcacttg accaacttgc
     3961 tgattccgga agaaccaaac ctaaaacggc aaacaaaacc atgaatgcca ttaaaataat
     4021 ccatctcttt ttcatgttac tatatgtttt aattaaaggc aaaattatta caaaatgaca
     4081 tagtatttta tatattttca ccaaaaggtt agtaaaatga ttttaatcat tgcaaagtga
     4141 taatatttta cgtaaaataa atgaagattg aaagcaaaat tagcaaatag cattacaatt
     4201 tgatatttgt gttggtgtga agaagggagg cttgcattat cttttcataa aataagctgc
     4261 gcctcggtaa atcgaaaata aattttcatt tactctcggc ttgcattatc tttgcctaag
     4321 aaaaaaaaag tttatcatga tatggaatga aactgctgaa tgcatggatc gcgaagaaat
     4381 gcgtaaattg caaagcgaaa gattaagaaa tgttgtagat agagtatact ataattgcga
     4441 accttatcgt aaaagaatgc aggaagccgg cgttatgccg ggtgatatcc aaacaataga
     4501 tgatatcgta aaacttccat ttacatcaaa aaaagattta agagactatt atccttttgg
     4561 attattatct tctcctatgt ctgaaatagt gcgagtacac gcgtcttcag gtacaacagg
     4621 taagccaatt gttgtaggct atactcgtaa tgatttaaat atatggaaag aagtaggagc
     4681 gcgttgtctt acagcattcg gtttaggtaa aaacgatgtc gttcaagtct cttatggtta
     4741 tggtttattt accggaggtc ttggcgctca tggagcagtc gaaacaattg gtggaaccgt
     4801 gatcccaatg tctgcaggaa atacagaaaa acaactattg atgatgcacg atttaggtgc
     4861 aaaggcatta gcttgtacac catcgtatgc tttatatttg gctgaaagta tggaaaaatc
     4921 cgaatttccg cgtgatgagt ttcagttgaa agtcggaata ttcggagcag agccgtggac
     4981 cgaacaaatg agacaagaaa ttgaacataa gatggggata aaagcctacg atatatatgg
     5041 tttgactgaa attatgggtc ctggagttgg tcacgaatgc caatttcaat gtggaacgca
     5101 cctttgtgag gatcatttct tccctgaaat tatcgatcct gaaacaggaa tacctgtagc
     5161 gccgggcgaa aaaggtgagt tagtgtttac aacacttaca aaagaaggaa taccaatgat
     5221 tcgttttaga acgagagact tgacccattt aatttatgat aaatgcgaat gtggacgtac
     5281 ttctgttcga atggctaaga taatgggacg ttgcgatgat atgttaatca tacgaggtgt
     5341 aaatgtgttc ccatctcaaa tagaagccgt aatatgcgaa accgaagaat ttgaaccgca
     5401 tttctttatc acagttgatc gtgttaacaa tacagacgtc tttgagattc aagtcgaagt
     5461 aaaagaacaa tattatacag atgatataag cggaatgctg aatctgcaaa aaaaattagc
     5521 gcataaaatt caaagtgtag ttggtataca acctacaatc agattggtag aacctcgttc
     5581 tttggaacgt agtcaaggta aagcaaaacg agtactagac aaacgcgttt ttaaataaac
     5641 actaaaaaag aaactgatat ggcaacaaaa caattatccg ttttcttaga aaacaaaacg
     5701 ggacgtctta atgatgtaac aaaaatatta gggcaggccg gcgttaatat gacggctttt
     5761 agtgtagcag ataattcaga ttttggaata ttacgtgtaa tagttcctga acccgaaaaa
     5821 gttcgcacat tactaaagga tagaggattt gcagtttctt taactgatgt tattgcagtt
     5881 aatattccaa ataaatcagg ttctttagat aaagtacttg atttgttggc ggaagcaaaa
     5941 atatacattg agtatatgta tgcattttca gaaggagaaa ccgcgagcgt agtaattaaa
     6001 cctgataata tggttcaagc ctctgaaatt ttacaaaaac ataaaataca cttgttgtga
     6061 atatgaatag accattaata ttggctacga atgacgatgg aatagatgcc cgtggtattc
     6121 attctttaat agaagcagtt acgcctcttg gagatgtcgt ggttgttgct ccggatggac
     6181 ctcgttcggc tcaatcaagt gctataactg taaatactcc tttaagaatt tggaaacatt
     6241 atgagaaaga tggtttagtg atgtatcgtt gtaatggaac tccaaccgac tgcgtaaaaa
     6301 taggattata taaagtactt gatcgtagac cggatgttgt agttgcaggt attaatcacg
     6361 gctcaaacac atcaattagt tctatctatt caggtacaat gggtgcagtt attgaaggtt
     6421 gcattgaagg cgtcccatct atagggtttt cattatgtga ttatgatccg cgtgccgatt
     6481 ttacagaagc agttcgttac gcggcaataa ttacgcaaaa ggtattagat gagggtttgc
     6541 ctaaagatat atgcttaaat gtgaatgttc ctcctgttga gcatgttaaa ggcatgaaag
     6601 tatgtagaca atgctatgga agatggaaag agcgtttcgt tataagagaa gatccggcag
     6661 gcaataatta ttattggtta acaggcgatt tctttgattt agaaacggat gcagaagata
     6721 ccgatgaggc tgcaatgaga aattcgtatg tatcagttgt accttgtaga gtcgatttga
     6781 ccgattatga tgcaataaat aaacttaaat catggaatta tgaagaaact agataatttt
     6841 tatctcggtc tagtctcttg tttattgctg ccatttttac tatttgtggt aacatggaat
     6901 atagtatccg gaactccatt gtctgaagta ccaatatgga tgcatcagcc ggttttttta
     6961 cagtatctga ttttttgttc attacccgat ttactaatct tgttttgggg ttatcatact
     7021 gaaagatgga atatatgtag aggtggagta ctcggactta ctccatattt aatgcttctg
     7081 ttttatttat tcttttaatg aaatattatt taatagcagg tgaggcatca ggagatttac
     7141 atggatctaa tttgatgaaa tctatacgag tcaacgaccc ggatgctgag tttcgttatt
     7201 acggcggaga tttaatgcag gcgcaaggag gcagtctagt gcgtcattat agggatactg
     7261 ctgttatggg tttcgtcagg gtagtgatgc atgctaaaac tatattgaag aatattcaac
     7321 aatgcaagga agatataaaa ctatatcaac ctgacgctgt tatactaatt gattatccgg
     7381 gatttaatct tcaaattgcg agatacgtaa aagagaagct gtctatacct gtcttttatt
     7441 atattcctcc caaaatatgg gcatggaaaa cgtctagagt aaaacttatt agaaaatata
     7501 ttgatagggt atatgcaatt tttccctttg aacccggctt ttatgcaaaa tttgcctgcg
     7561 aggcagtata tgtaggtaat ccaactgtcg agtctgtaag tcgtgcacat acatgtagta
     7621 tggcagattt tttgaagaaa aattcacttc ctgataagaa aattattgca ttgttgccgg
     7681 gtagtcgcaa acaagaagtt ggcctaacta tttcagtact taatgaaata gattttacac
     7741 aattcgaggg gtatcagtgc gtaatggcag caacatctgc agttgacatg agaatgtatc
     7801 agccggcaat taataaaggt attagaatcg tttacgacca aacctatgat ttgttggcaa
     7861 acgcctcaat tgcaatagta aattccggaa ctgccacact agaagcggca ctatttaatg
     7921 ttccgcaaat agtatgttat cctatacgaa ttccaggtgt tttatacgat tttggaagaa
     7981 aatatatgat caaaataccc tatatttcat tagtgaatat aatcgcaaat aagcaggttg
     8041 taaaagaact gcttgcttat caattttctg cgaaaaatac catctctgaa cttaagaaga
     8101 ttatccaaaa tgatgaatat cgaaatctta tgatgtctga ttacagacaa atttcatcta
     8161 atttaggctc ttctgttgca tcagatattg cttctcatga tatcatatct acaataaaaa
     8221 aacagagctt acaataatgt aaactctgtc agaatattta gtttgtactt tttagaaacg
     8281 gattccgtat gaaagtacaa tctggttacg tacacttctt aaatcaatcg gatctacaac
     8341 aagatcactt atataataca tagaactcga agggttcgaa aattcataat aatttgccat
     8401 gtttacttgg tatacatatg ccaaatcaat agcatgtcta cctatacgat aaccaacacc
     8461 agcagttata ttgtatgaac cattgtcatt atagtattca aaatcagtac gtgtatcatt
     8521 atcataatac aatcttgaac cattaaaaga ttcgcaagga gttgtatatc caccgccaat
     8581 tcttaaagat aatgcttcaa taggttttat ttcaacacct aaacgaacgg tgtgagtatc
     8641 caacatctcg ctaagtatga tatttttcat tgttacatta tccatacgtg atgatgattg
     8701 aatttccatt cccctatagt tggcatattg atagtcaatt ccaacgaacc cgaactttca
     8761 tagtacaaaa actaa
//
LOCUS       DAOPID010000041         8764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_10052_length_8764_cov_3.882650, whole genome shotgun sequence.
ACCESSION   DAOPID010000041 DAOPID010000000
VERSION     DAOPID010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 8764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8764
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10052_length_8764_cov_3.882650"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /gene="nqrF"
                     /locus_tag="PK641_02540"
     CDS             <1..1009
                     /gene="nqrF"
                     /locus_tag="PK641_02540"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HPG55265.1"
                     /translation="EILPTETVHFTRKQVKDNWRLGCQVKLKNDVSVKVPESVLGVKE
                     WECEVISNKNVATFIKEFIVALPKGEHMDFIPGSYAQIKIPEYTMDYSKDIDKELIGK
                     EYLPAWENFGLFTLKCTNTESTVRAYSMANYPAEGDRIMLTVRIATPPFKPKPEVGFQ
                     DVMPGIASSYIFTLKPGDKVIMSGPYGDFHPIFDTKNEMIWIGGGAGMAPLRAQIMHM
                     TKTLHTTDREMHYFYGARALNEVFYLSDFLGLEKEFPNFHFHLALDRPDPAADAAGVK
                     YTAGFVHNVMYETYLKNHEAPEDIEYYMCGPGPMSDAVVNMLDSLGVDPESIMFDNFG
                     A"
     gene            1098..3290
                     /gene="priA"
                     /locus_tag="PK641_02545"
     CDS             1098..3290
                     /gene="priA"
                     /locus_tag="PK641_02545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HPG55266.1"
                     /translation="MKEETLYADVILPIAVRTCYTYSFDAVNQCVSNGMRVRVPLGAK
                     KSYIGIVLRVHSDFNSEINYKSIIEVIDAQPIVTQSQLDLWAWISSYYLCSLGDVMKA
                     ALPGELAKNTYTPQTDILVACNDISDIKLTQKQKDLYDYVFGLCTDSEITKKQILSLY
                     TRSVYDALIKKELLREYEKKSSRLHSIEPCHDIYKLSDAQNKALLEIKQNFADSKPVL
                     LRGITSSGKTEIYTYLIKDVIASGKQVLYLVPEIALTTQLTNRLMQVFGDCMLVYHSK
                     ISDVQRAEIYTEIQKSNRYSVVLGVRSSVFLPFNNLGLIIVDEEHETSYKQYEPAPRY
                     NARNVALVLAKYTAANVLLGTATPSIESYYNTQIGKYALVELLERFENLQMPDVKVID
                     INDAFKKNRMQKMFSWFLIEKMKNTFANNKQVILFQNRRGFSSYIECQTCSWVPKCPH
                     CDLSLNYHKEKKRLICHYCGYSQEMPKVCPSCGKETIKDKGFGTEKVVDALNEFFEGV
                     QVGRLDVDTTRKKQDGDTILKNFESGKTNVLVGTQMLAKGLDFANVELVGILNADNML
                     NYPDFRAGERTFQLLLQVAGRAGRRHNDGLVVLQTSQPENPLINAIVKNDYLGFYESQ
                     LSERRLFMYPPYCKIINIYVKHKQQSTLNFYANSLANNLRKCLKDQVLGPEEPPVPKI
                     QQMYIKQIMLKIDNKISYQYVKEYIEKSINLIKAKNPSVICVVDVDPL"
     gene            3373..7611
                     /locus_tag="PK641_02550"
     CDS             3373..7611
                     /locus_tag="PK641_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HPG55267.1"
                     /translation="MKNLKIYSMGVDIGSTTIKVAILDDEGNLKFSDYRRHNANIRQA
                     AREIAIKMYHKLGNCKLDIVITGSVGMGYSSKLDYTFVQEVVASAEFVKQRYPQVHTF
                     IDMGGEDSKMIFFEVGKVPDIRMNGSCAGGTGAFIDQTASLLGISPAELSNLAESAET
                     IYPIASRCGVFSKTDIQNLISRSVSKNDIAASVFHAVALQVVASLARGTDIKPQVFFC
                     GGPFAFLPALKKSFIKVLELQNIDCVLPEHPQIIPALGCALLSADLAKRTVQLTEFLH
                     ILRFGKEITEDLYSDRLEPLFSDVNYEQWEKDKIKHLVQRAPLKEGVPCYLGVDSGST
                     TTKIVLLNQSGDLVYSDYRRNEGDSFLTFSKSLQDMHAKIGDVSILGTAATGYGENLL
                     KTAYNLDLGLVETIAHYTAARSITPNVSFVLDIGGQDMKAIFIDNGSISRIEINEACS
                     SGCGSFIETFASMLGYKVDKFAEMSCHAQHPCDLGTRCTVFMNSKVKQAMREGAKVED
                     IAAGFSYSVVKNCLFKVLKLKTVKELGSNIVVQGGTFKNHSIVRALEVLTGKNVAFSD
                     IPELMGAYGAALYAKESVKHHKERKLSDFIAANRYEPSIEVCQGCENHCTVKSFTFQN
                     GNTFYSGNNCERVFSNISEKKHRGVNMYAEKYRLLFNRISHKGGLRIGIPRGLGVFEN
                     FPFWHALFTNCGFEIELSKPSTDKVYGHGVRSIMADNICFPAKLMHGHVMDLIDKKVD
                     RIFYPYVVFEKKEDEKSQNSFNCPIVTGYSDVLKSSIDTAGRYGIQFDAPVITFNDEK
                     LLEASCLGYLKTLSVPKKKALRAIELAKKAQNEYLQTLTKRSMEVLEKANNEGRMVIL
                     LAGRPYHADPFIEHKISHAIADMGIDVITENIAVDEGSAIYNELNAVSQWAYPNRIFK
                     AASFVANSNMNLHYVELTSFGCGPDAFILDEVGSILKRRGKNITVLKIDDVNNIGSLR
                     LRIRSLVESLKLKSELEINYSDNKINNLSRTKVFTIEDRKRTIIAPYFAEGYSEFLPS
                     LFKLLGYNFVNLPMGNQEAANVGLKHANNEICYPSTIVIGSIIMALQSGKYNLDDIAV
                     IIPQTGGQCRASNYLALIKNAVVEAGYTNVPVLSLEIGGDLQNSQPGFTPDWKSIAKI
                     TIYVMLYADCINKLYYSSVVRENQKGISKRLKDKYTKAAFPYIEKRDYDGLLGLLKIA
                     VKDFTDAIDKEKKVARIGIVGEIYVKYNAFSNQYAVDWLINQGIEVVAPSIYNFFTMT
                     LVNSHINKEKHIKKEKLPLFAIDMIYMYIRHNVRKFDKICKAFPFYQSFSNIFKDEKL
                     ASKIVNTAANFGEGWLIPAELSNLAEHGVHNVVSMQPFGCIANHVISKGIEKRIKELY
                     PQMSLLFLDFDGGTSEANVFNRLHFMVENCKKTI"
     gene            7613..8221
                     /locus_tag="PK641_02555"
     CDS             7613..8221
                     /locus_tag="PK641_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPG55268.1"
                     /translation="MTTEEQILQTAEKLFLEKGFAMTSTTEIAKEVGCNQALVHYYYR
                     TKENLFLQVFMQKVRLFITAFTEKSGANDDFFERLEKRICAHANMMEQNPNLPFLIMN
                     ELILNKKRRCMIRDYIVDNPMVRDIYYEFDKEIKDEIAKGVIKPIDTLDLLLNVLSLN
                     VFIFVSAPIYTDILGRTDKEKQAYIAHRKKLVVQTILNSLKS"
     gene            8240..>8764
                     /locus_tag="PK641_02560"
     CDS             8240..>8764
                     /locus_tag="PK641_02560"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HPG55269.1"
                     /translation="MWYILLLSLLPSLLICIYIYFRDVREKEPFKLLLKAFFFGMLSV
                     ACTLFIHVVLNLAGLEYNNNAFMSAFVEAAIIEECSKFFLLLCLIWKNSEFNERFDGI
                     VYAVFVSMGFAFVENALYIFNDTNSYIPTSVARLLFATPAHFLFAVSMGYFVSVAKFN
                     PIKKKRNLVYALLSA"
BASE COUNT         2956 a         1242 c         1697 g         2869 t
ORIGIN      
        1 tgaaatccta cctactgaaa cagtacactt cacacgtaaa caagtaaaag acaattggcg
       61 tttaggatgt caagtaaaac ttaaaaatga tgtttctgtg aaggttcctg agtctgtgtt
      121 gggagtaaaa gaatgggaat gcgaagttat atcaaataaa aatgttgcaa catttattaa
      181 agaatttatt gttgcattac ctaaaggtga gcatatggac tttattccgg gttcttacgc
      241 acaaattaaa atacctgaat atacaatgga ttatagcaag gatatagata aagaattaat
      301 cggcaaggaa tatcttcctg catgggaaaa ttttggtcta tttaccctta aatgtactaa
      361 cacagaatct acagtgcgtg catattcaat ggctaattat cctgcagaag gcgatagaat
      421 tatgttgact gttcgtattg caacacctcc atttaaaccg aaaccggaag ttggattcca
      481 agatgtaatg cccggtatag catcttctta tatatttact ttaaaacctg gtgataaggt
      541 tattatgagt ggcccttatg gtgattttca tccaattttt gatacaaaga acgaaatgat
      601 atggataggc ggtggtgcag gtatggctcc gttacgtgct cagataatgc acatgacaaa
      661 gaccttacat acaacagatc gtgaaatgca ttatttctat ggcgcacgtg ccttgaatga
      721 agtattctac ttatctgatt ttcttggttt ggaaaaagaa ttccctaatt tccattttca
      781 tttagcactt gacagacctg atcctgcagc agacgcagca ggtgttaaat atactgcagg
      841 ctttgtacat aatgtaatgt atgaaacata tttgaagaat catgaagcac ctgaagatat
      901 tgaatattac atgtgtggtc ccggaccaat gtctgacgct gttgttaata tgttagatag
      961 tctaggagta gatccggaat caattatgtt tgataatttt ggtgcgtaaa tactatttta
     1021 aaaattactt caaaataagc tattggaaac aatggcttat ttttttttgt gcaatcatta
     1081 aatagtatct ttgttgaatg aaagaagaaa cattatatgc tgatgtaata ctgccgatag
     1141 ctgtgcgtac ttgctatact tatagttttg atgcagtaaa tcaatgtgtc agcaatggta
     1201 tgagagtacg agtgccgtta ggtgctaaaa aatcatacat tggtattgtt cttcgagtac
     1261 atagtgactt caattcggaa atcaattata aatctatcat tgaggtgata gatgctcagc
     1321 cgattgttac ccaatctcaa ttagatttat gggcttggat aagttcttat tatttatgct
     1381 cattgggtga tgttatgaaa gcggccttac ccggagaact cgcaaaaaac acatatactc
     1441 cgcaaacaga tatacttgtt gcttgtaacg atattagtga tattaaactt actcaaaagc
     1501 aaaaagactt gtacgattat gtgtttggat tatgtactga ttctgaaatt acaaagaaac
     1561 aaattctgtc tttatatact aggtcggtgt atgatgcatt aataaagaaa gaactgttac
     1621 gcgaatacga gaaaaaatca agccgtttgc atagtataga gccttgtcat gatatctata
     1681 aattatcaga tgcacaaaac aaagcacttc ttgaaattaa acaaaatttt gctgatagca
     1741 aaccggtttt gcttcgtgga ataacatcga gtggtaaaac tgaaatttat acatatctta
     1801 ttaaagatgt aattgcatca ggcaagcagg tcttatattt agtcccggaa attgctttaa
     1861 caacacaatt aacaaataga ttgatgcaag tttttggaga ttgtatgtta gtttatcatt
     1921 ctaaaatttc agatgttcaa agggcagaaa tatatactga aattcaaaaa tcaaacagat
     1981 attctgttgt attaggagta agatcgtccg tgtttttacc atttaataat ttaggtttaa
     2041 ttatagttga tgaggagcat gaaacttctt acaaacaata tgagccggct cctcgttata
     2101 atgcaagaaa tgtggccttg gtattggcaa aatacacagc tgcaaatgtt ttgcttggta
     2161 ctgcaactcc ttctattgaa agctattata atacacaaat aggtaaatat gctttagttg
     2221 aattattaga gcgttttgaa aatctacaaa tgcctgatgt taaagttatc gacattaatg
     2281 atgcatttaa aaaaaataga atgcaaaaaa tgttttcatg gttcttaata gaaaaaatga
     2341 agaatacttt tgcaaataat aaacaggtga tactattcca aaataggcgt ggtttttctt
     2401 cttatattga gtgtcagact tgctcttggg tgcctaaatg tccacattgt gatttaagtc
     2461 ttaattatca taaagaaaag aaacgtttga tatgtcatta ttgtggatat tcgcaagaaa
     2521 tgcctaaagt atgcccgtcg tgtggtaaag aaactataaa agataaaggt tttggtacag
     2581 aaaaggttgt tgatgctctt aatgagtttt ttgaaggcgt acaagttggt aggcttgatg
     2641 ttgacactac tcgtaaaaag caggatggcg acacaatatt gaagaatttt gaatcgggta
     2701 aaacgaacgt tcttgttgga actcagatgt tggctaaggg tttggatttc gctaatgtag
     2761 aattggttgg catcctgaat gctgacaata tgcttaatta tcctgatttt agagccggag
     2821 aaagaacttt tcaattgttg ctgcaagttg caggtagagc aggaagaaga cataatgacg
     2881 gcttagtagt tttgcaaact tctcaacctg aaaatccgct tattaacgcc attgtaaaaa
     2941 atgattatct tggattttat gaatcgcaat tatctgagcg taggttgttt atgtatcccc
     3001 cttattgcaa gattataaac atatatgtaa aacataagca acagtctaca ttgaattttt
     3061 atgcaaatag cttggctaat aacttacgaa aatgtttaaa agatcaagtc cttggaccgg
     3121 aagaacctcc tgtacctaaa attcaacaga tgtatattaa acagattatg ttgaagattg
     3181 ataataaaat ttcgtatcag tacgttaagg aatacatcga aaagtcaatc aatttaatta
     3241 aagccaagaa tccatcggtt atttgtgtgg ttgatgtaga tcccttatga tttttttgca
     3301 ataaaattaa tagacctatt aaataaatct attaattttg cgttatttaa gatatatcat
     3361 tatgattcaa atatgaagaa cttaaaaata tattctatgg gtgtggatat agggtccaca
     3421 acaattaagg ttgcaatatt agatgatgaa gggaatttga agttttcaga ttatcgccga
     3481 cataatgcaa atattagaca agcagcgcgt gaaatcgcaa taaaaatgta tcataaattg
     3541 ggtaattgta agcttgacat tgtcataaca ggctctgttg gcatgggtta ctcaagtaaa
     3601 cttgattata catttgttca agaagtcgtg gcatccgccg aatttgtaaa acaacgttat
     3661 cctcaagtcc atacatttat tgatatgggt ggagaagata gcaagatgat attttttgag
     3721 gtcggtaagg ttcctgatat tcgcatgaat ggtagttgtg caggtggtac aggggctttt
     3781 atagatcaaa ccgcatcatt attaggcata agtccggcag aattaagcaa tttggcagag
     3841 tctgccgaaa caatatatcc aatagcttcc agatgtgggg tattctctaa aacagatata
     3901 caaaatttaa ttagtagaag tgtcagcaaa aatgatattg ctgcatcagt ttttcatgct
     3961 gtagcattac aagttgttgc atcattggcg cgtggtacag atattaaacc tcaagttttc
     4021 ttttgtggtg gtccatttgc atttttacca gcattaaaaa agagctttat aaaggtattg
     4081 gaattgcaaa atatagattg cgttttaccc gaacatccac aaataatacc ggcattagga
     4141 tgtgcattgt tatcagccga tttggccaaa agaacagtgc agttaacaga atttttgcat
     4201 attttgcgtt ttggcaaaga aattacagaa gatttgtatt cggatagatt ggaaccattg
     4261 ttttcggatg ttaattatga gcaatgggaa aaggataaaa tcaaacattt agttcagaga
     4321 gctccattaa aagaaggagt gccgtgttat ttaggtgtcg attcaggctc tacaactaca
     4381 aaaattgtgt tgcttaacca atcaggcgat ttggtgtata gcgactatag aagaaatgaa
     4441 ggtgatagct tcctaacatt ttctaaatca ttgcaagata tgcatgcaaa gattggagat
     4501 gtttctattt taggaacagc tgctaccgga tacggcgaaa atttacttaa aactgcatat
     4561 aatttggatt taggtttggt cgaaaccata gctcattata cagctgcacg cagtattact
     4621 ccaaatgtgt cgtttgtttt agatattggc ggacaagata tgaaagccat ttttatagat
     4681 aatggttcta taagtcgaat tgaaattaat gaagcatgtt catccggttg cggctctttt
     4741 attgagacct ttgcaagtat gctaggatat aaggtagata agtttgccga aatgtcttgc
     4801 catgcacagc atccatgcga tttaggtact cgttgtactg tgtttatgaa ttcaaaagtg
     4861 aagcaggcga tgagagaagg tgcaaaagtc gaagatattg ctgcaggatt ttcttattcg
     4921 gttgtaaaaa attgtttgtt caaagtacta aagcttaaaa ctgtaaaaga attgggttct
     4981 aacatagttg ttcaaggtgg tacttttaaa aatcattcaa ttgtcagagc attagaagtc
     5041 ttgaccggta aaaatgttgc tttctccgat attcctgaat tgatgggtgc atacggagct
     5101 gctttgtatg caaaagaatc agtaaagcat cacaaggaac gtaaattatc cgattttata
     5161 gcggctaatc gttatgagcc gtcaatagaa gtttgtcaag gatgcgaaaa ccactgtact
     5221 gttaaatcat tcacattcca aaatggcaat acattttatt ccggaaataa ttgtgaacgc
     5281 gttttttcta atatttcaga aaaaaaacat cgtggggtga atatgtatgc agaaaaatac
     5341 cgtttacttt ttaacagaat atctcataaa ggtggcttga gaataggaat tccccgtggt
     5401 ttaggagtat ttgaaaactt tccgttctgg catgctttat ttacaaattg tggatttgaa
     5461 attgaattat caaaaccttc aacagataaa gtatacgggc atggtgtgcg ttcaattatg
     5521 gcagataata tttgttttcc tgcaaaattg atgcatggtc atgttatgga tttaatagat
     5581 aaaaaggttg atagaatttt ttatccctat gttgtatttg agaaaaagga agatgaaaaa
     5641 tcgcaaaaca gctttaattg tccaatagta acaggttatt ctgatgtttt aaagagttca
     5701 attgatacgg caggtcgtta tggtattcaa ttcgatgctc ctgttatcac ttttaatgat
     5761 gagaaactac ttgaagcatc atgccttggc tatttaaaaa cattatcagt tcctaaaaag
     5821 aaagctttac gtgcaattga attagctaaa aaagcccaaa atgagtattt acaaacactg
     5881 actaaacgtt cgatggaagt gcttgaaaaa gcgaacaatg aaggaagaat ggtaatttta
     5941 ttagccggac gtccgtatca cgctgatcct tttatagagc ataaaatatc tcatgcaatt
     6001 gcagatatgg gcattgatgt tataactgaa aatatagctg tagatgaagg ctctgcaata
     6061 tataatgaat taaatgcagt gtctcaatgg gcgtatccaa atagaatttt caaggcggca
     6121 agttttgttg ccaatagtaa tatgaatctg cattatgtag aattgacatc ttttggatgc
     6181 ggtccggatg cttttatact cgatgaggtt gggagtatcc taaaacgtcg tggtaaaaat
     6241 attactgtcc ttaaaattga tgatgtaaat aatataggtt cattacgctt aagaattcgt
     6301 tctttagtag aaagtttgaa attaaaatct gaattagaaa taaattattc ggataataag
     6361 ataaataatt taagtagaac aaaagtcttc actattgaag atagaaaaag aaccattata
     6421 gctccttact ttgcagaagg ttattctgaa tttttgccat cattattcaa attgttaggc
     6481 tataattttg ttaatctacc aatgggaaat caagaggcag ctaatgtcgg tctgaaacat
     6541 gcaaataatg aaatttgtta tccctctacg atagtaattg gctctattat tatggcattg
     6601 caaagcggca agtataatct tgacgatatt gctgtaatca ttccacaaac cggaggtcaa
     6661 tgcagagcaa gtaattactt ggcattgata aaaaatgcag tagtagaggc aggatatact
     6721 aatgtacctg tattatcact cgaaataggt ggagatttgc aaaattcaca accggggttc
     6781 actccggatt ggaaatcgat agcaaaaatt acaatatatg ttatgcttta tgccgattgc
     6841 ataaacaaat tatattattc atctgtagta agagagaatc aaaagggaat ttcaaaacga
     6901 cttaaagaca aatatacaaa ggccgccttc ccatacatag aaaaacgaga ttatgatggt
     6961 ttgttaggac ttttaaaaat tgcagttaaa gattttactg atgctattga taaagaaaaa
     7021 aaagtggcac gcattggtat tgttggtgaa atctatgtaa aatataacgc atttagtaat
     7081 caatatgcag ttgattggtt aattaatcaa ggaattgaag ttgttgcacc atcaatttat
     7141 aatttcttta ctatgacctt ggttaacagc catattaata aagaaaaaca tatcaaaaag
     7201 gagaaactcc cactatttgc tattgatatg atatatatgt atatacgtca taatgtaaga
     7261 aagtttgaca aaatttgtaa ggcattccca ttttatcagt cattttcaaa tatcttcaaa
     7321 gatgagaaat tggcatctaa gattgttaat actgcggcaa attttggaga gggttggtta
     7381 atacctgccg aattgtcaaa cttggcagaa cacggggttc ataatgttgt aagtatgcag
     7441 ccttttggtt gtattgctaa tcatgtaatt tcaaagggta ttgaaaaacg aattaaagaa
     7501 ttatatcctc aaatgagctt attatttctt gattttgatg gtggaacatc tgaagcgaat
     7561 gtatttaatc gcttacattt tatggttgaa aactgcaaaa aaactattta aaatgacaac
     7621 agaagaacaa atattacaaa ctgcagaaaa acttttttta gaaaaaggtt ttgctatgac
     7681 atcaaccact gaaattgcta aagaagtagg ttgtaaccaa gccttagttc attattatta
     7741 tagaactaaa gagaatctgt ttttgcaagt ttttatgcaa aaagtaagat tgtttataac
     7801 cgctttcaca gaaaaaagtg gagcaaatga tgattttttt gaacgtcttg aaaaaaggat
     7861 atgcgctcat gcaaatatga tggagcagaa tcctaatttg ccgttcctta ttatgaacga
     7921 gttgatcttg aataaaaaga ggcgttgtat gattcgagat tatattgttg acaatccgat
     7981 ggttagggat atctattatg agtttgataa agaaattaag gatgaaatag caaaaggagt
     8041 tataaaaccg attgatacat tagatttact attaaatgtt ttgtcgctaa atgtttttat
     8101 ttttgtatct gccccgattt ataccgacat attaggtaga acagataaag aaaagcaggc
     8161 gtatattgcg cataggaaaa agcttgtggt tcaaaccata ttaaatagct taaaatctta
     8221 aaatataaaa atgtaattta tgtggtatat tcttttacta tcgcttttac cttcgctttt
     8281 aatttgtata tacatatact ttcgtgatgt acgagaaaaa gagcctttta aacttctgct
     8341 taaggccttc ttttttggta tgttatcagt agcatgtaca ctatttattc atgtagtgct
     8401 taatttagcg ggtcttgagt ataataacaa tgcgtttatg tctgcgtttg tcgaagctgc
     8461 aattatagaa gaatgtagta agttcttttt actattgtgt ttgatatgga aaaattctga
     8521 atttaacgag cgttttgatg gtatagtgta tgcagtattt gtgtcgatgg gttttgcatt
     8581 tgtagagaat gctttgtata tatttaatga tactaatagc tatataccta cttctgttgc
     8641 caggctttta tttgcaacac cggcacactt tttatttgca gtttcaatgg gctattttgt
     8701 aagtgttgca aaatttaatc caataaaaaa gaaacgtaat ttagtgtacg ctttgctttc
     8761 tgca
//
LOCUS       DAOPID010000042         8678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_10193_length_8678_cov_5.009857, whole genome shotgun sequence.
ACCESSION   DAOPID010000042 DAOPID010000000
VERSION     DAOPID010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 8678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8678
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10193_length_8678_cov_5.009857"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1306
                     /locus_tag="PK641_02565"
     CDS             <1..1306
                     /locus_tag="PK641_02565"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HPG55270.1"
                     /translation="NRDIFMDMLTDSGVNAMSDRQQAAMLVADDSYAGSETYNRLTNK
                     LTELFGTKYFLPTHQGRAAENILAEAFVRPGMVTLMNFHFTTTKAHITRMGGSVEELI
                     IKEGLEPNNNNPFKGNFDLDLLRKRIKEVGPKNVAFVRIEAGTNLVGGQPLSMENMLA
                     VSDICKEYGVMSVLDASLLQDNLYFIKVREAAAKDMSIPQITRKLADAMDIIYFSARK
                     LGFARGGGIAMRDENHCLKMKEFVPLYEGFLTYGGMSVREMEAITVGLDETMDFDIIS
                     QGPQFIKYMCDELEKYGVPVITPAGGLGCHLNASEILPQLPHNQYPAAALGSALYIAG
                     GVRGMERGTLSEQREADGTERYAELELLRLAVPRRVYTLSQINYAVDRIKWLFDNRNL
                     IGGLEFYEEPKVLRFFFGRLRPTSDWQEKLVAKFRKDFGDSL"
     gene            complement(1437..3257)
                     /locus_tag="PK641_02570"
     CDS             complement(1437..3257)
                     /locus_tag="PK641_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPG55271.1"
                     /translation="MENKNVHLSHLVFTQAKKYGDRPAMYFRDDNTKTWLPITWNESA
                     RQVRLIGKALISIDVKEGDRVGIFSQNMYEVIIADFANFAAKAASVPLYATSTAPQIE
                     YIVNDAEIEYLFVGEQYQYDVAMEVQKTSKFIKKLIVIDPKVDLKGNKNAIRYSDLLL
                     VGEKANNDAEIDRRLAKTSEDDLMNILYTSGTTGTPKGVMLHQSNFTEAIRIHEERLA
                     ITTDNDTSMASLPLTHVFERTWDYFCLGHGVRVYINRSPAEIQTTILEVRPTLMCAVP
                     RYWEKVYAAINAKIESTPKLMQLFFKWAISIGRKRNLNYKRFGKRTPPDLGTRYKFFA
                     KPLFKMVHKAAGLENCKYFPCAGARLSDEINEFMHAIGINVCYGYGLTESTATVSCFF
                     PENRDYVIGSIGRIMPNLQVKISEEGEILLKGKTITAGYYKRPDANKEAFTSDGWFKT
                     GDAGYIDMDNNLYITDRIKDLFKTAGGKYIAPQLLEGIICDDKFVEQAAAIGNERKYV
                     TMLIVPAFDILEPYAKEKGIKYESRADLIQNPEIVALYEDVIKNRTKELAKYEQIKKF
                     TLLVKPFTMEQGHLTNTLKLKRKVINELFSKEIAAMYTED"
     gene            complement(3268..4032)
                     /gene="rnc"
                     /locus_tag="PK641_02575"
     CDS             complement(3268..4032)
                     /gene="rnc"
                     /locus_tag="PK641_02575"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPG55272.1"
                     /translation="MLGFTPINMEYYNLALLHRSTYIKTATGDTLNNERLEYLGDAIL
                     SAAVSDILYKEFPNKKEGELTCIRSRLVQRSTLDDLAVKLGLDRMVISDKKSTQNFQK
                     VHINGNALEALMGAIYLDRGFAKCKFFINKIVLEKYIDLYQTVENEVNFKSRFIEWAQ
                     RYKYQYNFIVADVDYDKAHNISNFHSKIEVEGIIVGNGHGLSKKESQQEAAKKAMQTI
                     KTKSFKAQLKKLKEEEEITEPITETKKRTLKKTTTD"
     gene            complement(4130..5386)
                     /gene="fabF"
                     /locus_tag="PK641_02580"
     CDS             complement(4130..5386)
                     /gene="fabF"
                     /locus_tag="PK641_02580"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HPG55273.1"
                     /translation="MELKRVVVTGLGALTPIGNNVPDFWSALLSGVSGAGPITRFDTS
                     KFKTQFACEVKGFDANQHFDRKELKKHDLYTQYAIVAARQAILDSNIDKEKEDLDRIG
                     VIFGVGIGGLSTFEQEFCEFAKGDGTPRFSPFFIPKMIADIACGHISMEYGFRGLNFA
                     CVSACASGTNALTNAYNHIRLGKADIIVAGGAEAPITQGGVGGFNSMHAISTRNDSPQ
                     TASRPFSKSRDGFVMGEGAGCLILEELEHAIKRGAKIYAEVAGEGASADAYHLTASHP
                     EGAGAILVMKNALKDANMTPEDIDYINVHGTSTPVGDISEVKAIKQVFGEHAYKLNIS
                     STKSMTGHLLGATGAVETIACVLSVQNDIVPPTINFTGEEDEEIDYRLNFTFGKAQKR
                     IVRAALSNTFGFGGHNASIIVKKFVK"
     gene            complement(5404..5640)
                     /locus_tag="PK641_02585"
     CDS             complement(5404..5640)
                     /locus_tag="PK641_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPG55274.1"
                     /translation="MSEVASKVKAIIVEKLGVEESEVVPEASFTADLGADSLDTVEMI
                     MEFEKEFGIQIPDDQVEKIATVQDAINYIEANGK"
     gene            5885..6454
                     /locus_tag="PK641_02590"
     CDS             5885..6454
                     /locus_tag="PK641_02590"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009039116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HPG55275.1"
                     /translation="MKKVAIFASGSGSNAENIINYYKDSREIFFPLILTNKADAYICE
                     RAKNLNVPCIVFQKQDMSNGVLLKILKDADIDFIVLAGFLLKVSDEIIAAYPKKIVNI
                     HPALLPKFGGKGMYGDRVHEAVVEAGETESGITIHYIDNHYDAGTIIFQAKCEVLPSD
                     KPSDVATKVHALEYANYPRVIGECILRDA"
     gene            6558..>8678
                     /locus_tag="PK641_02595"
     CDS             6558..>8678
                     /locus_tag="PK641_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005835115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPG55276.1"
                     /translation="MMSFVHLHVHSHYSVLDGMSKVPDLINKAVACGMPAIALTDHGN
                     MFGIKEFIDEVNRLNAKPKKELAECRKELEKASNEEERAKLEIKCAELKAKIDSYIPF
                     KGIVGVEAYCARRTLFDKDKNVKTVHPESKKEYIVDSSGYHLILLAKNKKGYQNLCKL
                     VSQSWIDGYYSRPRIDKNILEKYKEGLIICSACLGGELPQLIMQNKVDEAEKSVRWFK
                     SVFGDDYYIEIQRHKTNKPNGDQNVYERQQQINPILLDLARKTNTKIIASNDVHFVEE
                     SHSEAHDRLICLSTGKYLDETDRMHYTKQEWLKTPDEMSAIFSDLPEAISNTLEIADK
                     IEPYSIESGPIMPKFDIPKEFGTEEDYHKKFTDQDLFEEFTCNEKGEVVLSKEDADNK
                     IKKLGGYEKLYRIKLEADYLADLAWKGAAIRYGKELTEEQRERIIFELHVMKTMGFPG
                     YFLIVCDYIRAAREELGVSVGPGRGSAAGSVVAYCLKITDIDPLKYDLLFERFLNPDR
                     ISLPDIDVDFDDDGRGAVLDWVTNKYGKEKVAHIITYGTMATKSSIKDVGRVQRIPLS
                     TVNEVVKLIPDKFGDNLADPKTKNIPKVNVKNCLKYVPELQQLRNGSDEEISSMLEYA
                     EELEGTIRQIGIHACGVIIGADDLTNFTPLSTVKDKETDQDVLVTEYDGRVIESVGLI
                     KMDFLGLSTLSIIKEALLNIKKSRG"
BASE COUNT         2707 a         1456 c         1614 g         2901 t
ORIGIN      
        1 aaatcgcgac atctttatgg atatgttaac cgatagtggt gttaatgcaa tgagtgatcg
       61 tcaacaagca gcgatgttag tagcagacga tagctatgca ggcagcgaga cttataatcg
      121 tttaacaaat aaacttacag aactttttgg tacaaaatat tttctcccta cacatcaagg
      181 tcgtgccgct gagaatatat tggcagaagc ttttgtaaga ccaggaatgg taactttgat
      241 gaacttccac tttacaacaa caaaagctca cattactcgt atgggaggtt ctgttgaaga
      301 attaattatc aaagaaggtt tggaaccaaa caataataat ccttttaaag gaaactttga
      361 tttggattta ttgcgtaaac gtattaaaga agtaggacct aagaatgtag cattcgttcg
      421 tatcgaggca ggcacaaact tagttggtgg acaaccactt tcaatggaga acatgctggc
      481 tgtatcagat atttgtaaag agtatggtgt aatgagtgta ctagatgcat cattattaca
      541 agataactta tactttatca aagtacgtga agcagctgca aaagacatgt caattccaca
      601 gattacacgt aaattggcag atgcaatgga tattatttat ttctcggcac gtaaattagg
      661 ttttgctcgt ggtggtggta ttgcaatgcg tgatgaaaac cattgtttaa aaatgaaaga
      721 atttgttcca ttgtatgaag gattcttaac ttatggcggt atgtctgttc gcgaaatgga
      781 agctataact gttggtttgg atgaaacaat ggattttgat attatatcac aaggacctca
      841 atttattaaa tatatgtgtg atgaattgga gaaatatggt gttcctgtta taactcctgc
      901 aggtggcttg ggttgtcact tgaatgccag cgaaatattg cctcaattac cacataatca
      961 atatcctgct gctgcattag gttctgcatt atatatagca ggtggtgttc gtggtatgga
     1021 acgcggtaca ttgtcagagc aacgtgaagc agatggtaca gaacgttatg ctgaattaga
     1081 attacttcgt ttagcagtac ctcgtcgtgt atatacattg tctcaaataa attatgctgt
     1141 tgaccgtatt aaatggttat ttgataatcg taatttaatt ggaggcttgg aattctatga
     1201 agaaccaaaa gtacttcgtt tcttctttgg tcgtttgcgt ccaacttcag attggcaaga
     1261 aaaattagtt gctaaattcc gtaaagattt tggtgacagt ttataattgc tgattttggc
     1321 agattttgcg agaagcatat attcggccta agcaattaat attagaatat atataaacaa
     1381 aaaggatgat tctcaaccga gaatcatcct ttttgttttg caattaaagc atataattag
     1441 tcttcagtat acatagctgc aatttctttt gaaaatagct cgttgattac cttacgttta
     1501 agttttaatg tatttgtaag gtgtccttgc tccattgtaa aaggtttaac aagaagagta
     1561 aatttcttaa tctgttcgta ttttgccaat tctttggttc tatttttaat aacatcttcg
     1621 tataaagcaa caatctcggg attttgtatt aaatctgcac gtgattcgta tttaatacct
     1681 ttttcttttg catatggctc aaggatatcg aaagccggta cgataagcat tgtaacatat
     1741 ttacgttcat tacctattgc agcagcttgc tctacaaatt tatcatcaca aataatacct
     1801 tctaacaatt gtggtgcaat atattttcca ccggcagttt taaataaatc tttgatacgg
     1861 tctgtaatgt ataaattgtt gtccatatca atatagccgg catctccggt tttaaaccaa
     1921 ccatcacttg taaatgcttc tttgtttgca tccgggcgtt tataatatcc ggctgtaata
     1981 gtcttgcctt ttagtaatat ttcaccttct tccgaaattt ttacttgtaa gtttggcata
     2041 atacgaccaa ttgaaccgat tacgtaatca cgattctcag ggaagaaaca tgatacagta
     2101 gctgttgatt ctgttaagcc gtatccgtag caaacattta tgccaatagc gtgcataaat
     2161 tcatttattt catcagacaa gcgtgcacct gcgcatggaa aatatttaca gttttccaaa
     2221 ccggccgcct tatgtaccat cttaaacaaa ggcttagcaa aaaacttgta tcgtgttcct
     2281 aaatcaggag gcgtacgttt accaaagcgt ttataattca gatttctttt acggccaata
     2341 gaaatagccc atttaaagaa taattgcatc agtttaggcg tagattctat ttttgcattt
     2401 atagcagcat aaactttttc ccagtatctt ggcacagcac acattagggt agggcgtacc
     2461 tctaaaatgg ttgtctggat ctctgcaggt gagcggttga tgtacactct cacgccgtgg
     2521 cctaaacaaa aataatccca tgtacgttca aatacgtgtg ttaatggcaa ggatgccata
     2581 gaggtatcat tatctgtagt aatagcaagt ctttcttcgt gtatgcgtat tgcctcagta
     2641 aaattagatt gatgtaacat tacgccttta ggagtacctg tagttcctga tgtatataaa
     2701 atgttcatca agtcgtcttc tgaagttttt gctaaacgtc tatctatttc tgcatcattg
     2761 ttagcttttt cgcctacaag cagtaaatca ctgtatctta tggcattttt attgcctttt
     2821 aaatccactt taggatctat tacaatcaac ttctttatga attttgaagt tttttgaact
     2881 tccattgcaa cgtcatattg atattgttct ccaacgaaca gatactcaat ttctgcatca
     2941 tttacaatat attcaatttg aggagcagtt gatgttgcat ataatggaac agatgctgct
     3001 tttgccgcaa aattggcaaa atctgcaata ataacctcat acatattttg tgaaaaaata
     3061 cctacgcggt cgccttcttt tacatctatt gaaattaatg ccttaccaat taaacgaact
     3121 tggcgtgcag attcattcca tgttataggt aaccatgttt tagtattatc atcgcgaaaa
     3181 tacatagcag gacgatctcc atatttctta gcttgagtaa atactaaatg tgacagatga
     3241 acatttttat tttccatttt gtgtaaatta gtcagttgtt gtttttttta gtgttctctt
     3301 tttagtctca gttattggtt ctgtaatttc ttcttcttct ttcagttttt ttaattgagc
     3361 tttaaaagat tttgttttaa tggtttgcat agcttttttt gcagcttctt gttgcgattc
     3421 ttttttgctt aaaccatgtc cgttaccaac tattattcct tctacttcaa tttttgaatg
     3481 aaaatttgaa atattgtgtg ctttatcata atcaacgtca gcaacaataa aattatattg
     3541 atacttataa cgttgtgccc attcaataaa tcgtgattta aaatttacct cattttctac
     3601 tgtttggtat aaatcaatat atttttcaag tacaatttta ttgataaaga acttacattt
     3661 agcgaagcct ctgtcaagat aaatggctcc cattaatgcc tctaaagcat ttccgttgat
     3721 atgtactttt tgaaagttct gtgtactctt tttgtcggat ataaccatac gatctaatcc
     3781 taatttaacg gcaagatcat ctaatgttga gcgttgaacc aaacgggagc gaatacaggt
     3841 taattctccc tctttcttat ttggaaattc tttgtataga atatcagata cagccgcgct
     3901 taaaatcgca tcgcccaagt attctaaacg ttcattattt aaggtatcac ctgttgctgt
     3961 ctttatatat gtagaacgat gtagtagagc taggttgtag tactccatat taataggagt
     4021 aaaaccaagc atttttttta aatggaaata aggttcttcc ttttttgaaa aaaagaacct
     4081 tattttcgat tttatttctt ttatcaagat gataaatcgt ttaatcggat tatttgacaa
     4141 atttcttaac aatgatgctc gcgttgtgtc ctccaaatcc aaaagtattg gataatgctg
     4201 cacgaacaat gcgtttttgt gctttgccaa atgtaaagtt aagacgataa tctatttctt
     4261 catcttcctc acctgtaaaa ttaatcgtag gtggtacaat gtcgttttgt acagacaaaa
     4321 cacaggctat ggtctcaaca gcacctgttg cacccaataa gtgtccggtc attgattttg
     4381 ttgaactgat attgagtttg taggcatgtt cgccaaaaac ttgtttaatg gctttcactt
     4441 cagagatatc tcctacgggt gtagaagttc cgtgaacatt tatgtaatca atatcctcag
     4501 gagtcatatt ggcgtccttc aaggcattct tcattactaa aatggctcct gcaccttccg
     4561 gatgtgatgc tgttagatgg taggcatcag ctgatgcgcc ttctccggct acctctgcat
     4621 aaattttggc acctcgttta atggcatgct ccaattcttc taaaataagg catcctgcac
     4681 cttcacccat tacaaagcca tcgcggcttt tgctaaaagg acgtgatgct gtttgagggc
     4741 tatcgttgcg tgtagaaatt gcgtgcatag agttgaatcc gccaactcca ccttgtgtaa
     4801 taggtgcctc agcaccacct gctactataa tatcggcttt tcccaaacga atgtggttat
     4861 aagcatttgt taaagcatta gttcctgatg cacaagctga tacacatgca aaattcagac
     4921 ctctaaatcc atattccatt gaaatatgtc cgcatgcaat atctgcaatc attttgggaa
     4981 tgaaaaacgg actaaaacgt ggtgttccgt ctccttttgc gaattcgcaa aattcttgtt
     5041 cgaaggttga taaacctcca ataccaactc caaaaataac accgatgcga tctaagtctt
     5101 ctttttcttt atcgatgttt gaatctaaga tggcttgcct tgccgctaca atggcatatt
     5161 gagtatataa atcgtgtttt ttaagttctt ttctatcgaa gtgttgattt gcgtcaaaac
     5221 ctttcacttc gcaagcaaat tgtgttttga attttgaagt atcaaaacga gtaataggtc
     5281 cggctccact cacaccactt aacaaagcac tccagaaatc cggaacgtta ttgcctattg
     5341 gtgtcagtgc acccaaacct gttactacta ctctttttaa ttccataaaa tgcaaagagc
     5401 tgtttatttt ccgttagctt cgatgtagtt gatagcgtct tgtacagttg cgattttttc
     5461 tacttggtca tcaggaattt gaataccgaa ctctttctca aattccataa tcatttctac
     5521 tgtgtccaat gaatcagctc ctaaatcagc tgtgaagctt gcttcaggaa caacttctga
     5581 ttcttcaacg cctaactttt ctacaatgat agcttttact ttagatgcta cttctgacat
     5641 aaccttaaat tttagttagt aaatagttta aatttttgtg ctgcaaagat aagtcatttt
     5701 atgtaaactg cagctatttt gaaattaaaa tatgcacaaa acaaccaaaa atgtgcaatc
     5761 attattatgc gagatattaa aattggtact taattatttg atttttaatt tgttgataaa
     5821 atgttaaatg ttgataactt ttttttttga atactttgct gtacatttgt gcaaaattat
     5881 aatcatgaaa aaagttgcaa tttttgcatc cggttcagga agtaatgctg aaaatattat
     5941 aaactactat aaagatagta gagaaatatt ctttcctctt attctcacta ataaagctga
     6001 tgcatatata tgcgagcgcg caaaaaactt aaatgtacca tgtattgttt ttcaaaaaca
     6061 agatatgagt aatggtgttt tgctgaaaat attgaaagat gcagacatcg attttatagt
     6121 attagccggt ttcttgttaa aagtatcaga cgaaattata gcagcatatc ccaaaaaaat
     6181 agtaaatatt catcctgcac ttttacctaa attcggtggt aaaggtatgt atggagatag
     6241 ggtgcacgaa gcggttgttg aggccgggga aacagaaagt ggcatcacca tacattatat
     6301 agataatcat tatgatgccg gaactattat ttttcaggca aaatgtgaag ttcttccatc
     6361 cgataagcct tctgatgtgg caactaaggt ccatgctcta gaatatgcta attatccgag
     6421 agttataggt gaatgtatac ttagagatgc ctagattgct tgttcataac ttttagttaa
     6481 aagtcttttt ttatagcttg tattctctgt atctttgaaa ttctgacaaa caaaaaacag
     6541 tcagaattaa agatttgatt atgagttttg tacacctaca cgtccactcc cactattctg
     6601 ttttagacgg aatgtctaaa gttcctgatt taattaataa agcagttgct tgcggtatgc
     6661 cggctattgc tcttaccgac catggaaaca tgttcggtat aaaggaattt atagacgagg
     6721 ttaatagatt aaatgctaag cctaaaaaag aattagctga atgtcgtaag gaacttgaaa
     6781 aagcttcaaa cgaagaggaa agggctaagt tggaaataaa atgtgccgag ttgaaggcaa
     6841 aaatagattc atatatacca ttcaaaggaa tagttggagt tgaagcatat tgcgcccgtc
     6901 gtactttgtt tgataaagat aaaaatgtaa aaactgtaca tcctgaatca aaaaaagaat
     6961 atattgttga ctcgagtggt tatcacctta tattattagc aaaaaacaaa aaaggatatc
     7021 aaaatctgtg taagcttgtt tctcagtcgt ggattgatgg atattatagt aggccccgta
     7081 tagataagaa tatattagaa aaatataaag aaggtttgat tatatgttct gcttgcttag
     7141 gtggtgaatt accgcaactt ataatgcaaa ataaggttga tgaggctgaa aaatcagttc
     7201 gctggtttaa aagtgtattt ggcgatgatt actatattga gattcaacgt cataagacga
     7261 ataaacctaa tggagatcaa aatgtttatg aaaggcaaca gcaaataaat cctattttgc
     7321 ttgatttggc gcgtaaaacg aatacaaaaa taattgcttc taatgacgtg cactttgtag
     7381 aggagtcgca ttcggaggct catgatcgtt taatctgttt aagtacaggt aaatatttag
     7441 atgaaaccga tagaatgcat tataccaaac aagagtggtt aaaaacaccg gacgaaatgt
     7501 ctgctatttt ctcagatttg ccggaagcta tctcgaacac tcttgaaata gccgataaaa
     7561 ttgagccgta ttcaatcgaa tcaggtccaa ttatgcctaa atttgatatt cctaaggagt
     7621 ttggtaccga agaagattat cacaaaaaat tcaccgatca ggatttattc gaagaattta
     7681 cttgcaacga aaagggtgaa gttgtattgt cgaaagaaga tgcagataat aaaattaaga
     7741 aactcggtgg ttatgagaag ttgtatcgta taaagttgga ggctgattat ttggctgatt
     7801 tagcatggaa aggtgctgca atacgttatg gcaaagaatt gacagaggaa cagcgagaac
     7861 gtataatatt tgaattgcac gtaatgaaaa ccatgggttt tccgggctat ttccttattg
     7921 tatgtgatta cattcgtgcc gcacgcgaag aacttggcgt aagtgtagga cccggacgtg
     7981 gatctgcagc cggttcggta gtagcttatt gcttaaaaat tacagatata gatcccttaa
     8041 aatacgattt gctttttgaa cgtttcttaa acccggatcg tatttcatta cctgatatag
     8101 atgttgattt tgatgatgat ggacgaggag cagtgcttga ttgggttaca aataaatatg
     8161 gcaaagaaaa agttgctcat attattacct acggaaccat ggctacaaag tctagtataa
     8221 aagatgtagg gcgagttcaa agaataccat tgtcaacagt aaacgaagtt gtaaaactta
     8281 ttcctgataa gtttggggac aatttagctg atccaaaaac gaaaaacatc ccaaaggtta
     8341 atgtcaaaaa ttgcttgaaa tatgtgcctg aattgcaaca actgcgaaat ggctcagacg
     8401 aagagatttc atcaatgctt gagtatgccg aagaacttga gggaacgatt agacaaatag
     8461 gtatacatgc ttgtggtgtg attatcggtg ccgacgactt gacaaatttt actcctttaa
     8521 gtactgtaaa ggataaagaa actgatcaag atgtattggt taccgagtat gatggtcgcg
     8581 taattgaatc ggttggttta attaaaatgg acttcttagg attaagtaca ctatcaataa
     8641 taaaggaggc tttgttaaac ataaagaaat caagagga
//
LOCUS       DAOPID010000043         8563 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_10398_length_8563_cov_4.225905, whole genome shotgun sequence.
ACCESSION   DAOPID010000043 DAOPID010000000
VERSION     DAOPID010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 8563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8563
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10398_length_8563_cov_4.225905"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PK641_02600"
     CDS             <1..447
                     /locus_tag="PK641_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HPG55277.1"
                     /translation="VIYQKNLIEAVNDNVKRVSAVLSVFSILLLLISYALISNTVRLS
                     VYAKRFLIHTMKLVGATSSFIRRPFLLQGAITGILSAVIAMGMLSAILFWAQSEISTL
                     LTTEMLNTLLVSFFVMILSGVVITIIATYLSVSRYLRLTIDDMYYI"
     gene            484..759
                     /locus_tag="PK641_02605"
     CDS             484..759
                     /locus_tag="PK641_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HPG55278.1"
                     /translation="MSNNNEDNLKFRIARKNLWLMGIGVLIIVVGLALMLIGNNSGPD
                     TFEPDIFSFRRIVVAPMVVFFGFLSIIVAICYRPKQNKNNLWNGMKP"
     gene            738..1532
                     /locus_tag="PK641_02610"
     CDS             738..1532
                     /locus_tag="PK641_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008666468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HPG55279.1"
                     /translation="MEWYEALVLGLLQGLTEFLPVSSSGHLEIGQVLFGTAGEENLTF
                     AIVVHAATVLSTIVILWNEIKLLFKGTFTTKAWNPEKTYIAKLIVSMIPVFIVGVFFK
                     DAVESFFGNGLLLVGICLLVTALLLAFAYFAKPRQRNEISYIDAFIIGLSQAVAVLPG
                     LSRSGTTIATGLLLGNKKENMAQFSFLMVLVPILGEAFLEFIDLAKEGAPMGISYMSL
                     IIGFIAAFVSGCIACRFMINIVKKGKLIYFAVYCVIVGGIAIAYSL"
     gene            1534..2232
                     /gene="truB"
                     /locus_tag="PK641_02615"
     CDS             1534..2232
                     /gene="truB"
                     /locus_tag="PK641_02615"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPG55280.1"
                     /translation="MDFKEGEILCFDKPLHWTSFLLVNRVRWAITNYLGVKKLKVGHA
                     GTLDPLATGVMILCTGKATKRIDEFQYQTKEYEAVLKLGATTPSFDLEKEIDATYPTE
                     HITEELLRNVLSDFVGEIRQVPPAYSAVKINGKRAYEYARKGDDVEINAKPLLIDSIE
                     LLEFSMPIVKIRVVCSKGTYIRALARDIGVALQSGAHLVGLRRTRVGDITLDKCMSFE
                     DFKSMIDTLPAIVN"
     gene            2269..3318
                     /gene="queA"
                     /locus_tag="PK641_02620"
     CDS             2269..3318
                     /gene="queA"
                     /locus_tag="PK641_02620"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HPG55281.1"
                     /translation="MKLSQFKFKLPEELLAQKPSANRDESRLLVLKRQTGEIEHKVFK
                     DILGYFDENDVFVFNDTKVFPARLYGNKEKTGARIEVFLLRELNPDLRLWDVLVDPAR
                     KIRIGNKLYFGEDDSLVAEVIDNTTSRGRTLRFLFDGDHDTFKQTLYSMGETPLPRFI
                     KRPVEPEDTERYQSIFAKNEGAVVAPASSLHFSRELMKRLEIKGVNFSFVTMHAGLGN
                     FREIDVEDLTKHKMDSEQMIVTDQAASVVNVAKDEKRQVCAVGTTVMRALETAVCTEG
                     KIKPFDGWTNKFIFPPYDFTVATSMVSNLYSPYSQLLMLVAAFGGYDQVMHAYDVAVK
                     EKYQFGAYGDAMLII"
     gene            3447..6518
                     /gene="secDF"
                     /locus_tag="PK641_02625"
     CDS             3447..6518
                     /gene="secDF"
                     /locus_tag="PK641_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HPG55282.1"
                     /translation="MQNKGFVKLFAIALALVCLFYLSFNAVTAHYNSKAAEYANGNKM
                     NEYHYLDSMATEKVWLGYTLKECRENELNMGLDLKGGMNVILEVSVPDIVRTLSGNTK
                     DETFNKAINLAIERQAVSQKDFLVLFQEAYEEIDPNARLSAVFTTFDLKDRIFLKSTN
                     KEVIDVLKQEVQATVDNSFNVLRTRIDRFGVVQPNIQRLETNGRILIELPGIKEPERV
                     RKLLQGSASLEFWETYNLTEIYSSLELANAAVREYLKGADTTSVEKNTEKAKAEVAKS
                     EKVVSESDSIKVQLEKIGAAAQDSSKMIEQFAKENPLFSIMMINQSGGQLIPTPAIAM
                     VSIRDTAKVNEYLNLRQVKEILPRDLAFRWTVKPIDEKGLIYQLVAIKITNRDGKAPL
                     GGNVITDARNDLSQFSANSTVSMTMNPEGAKTWARLTKENIGRSIAIVLDNMVYSFPT
                     VNTEISGGRSEISGNFTTEEAKDLANVLKSGKMPAPSRIVQEDVVGPSLGHEAITQGL
                     WSFLIAFIVVMLYMILMYGLVPGLIVDVALLANVFFLVGILASFKAVLTLPGIAGIVL
                     TMGMAVDANVLIYERIKEELAAGKTHKSAVSDGYKNALSAIIDSNLTTLLTGVILFYF
                     GTGPIKGFATTLIIGIITSFFTAVFITRVIYDKMVDNGSVSKLTFVTSFSKNLLQGVN
                     VNWIKIRKFGYILSCAFVLIAIVSLSVRGLNQGVDFTGGRNYVVRFAEKVNTEEIQQM
                     LAPGFTDASVSVITIGDENQVRISTNYKIADNSKTIDDEVEDIIYKSLQSKLAKDGVS
                     KEMFIKGYIIDNGKANLSTDANVSSLGVQSSQKVGPAIADDIKNAAFMAVVFSLLVVF
                     LYILVRFRNVSYSVGALSSLVHDTIFILGIYSLTYTIMPFSMEIDQSFIAAILTVIGY
                     SINDTVVVFDRVRELKTNFPKRNQKDVINEAVNATLIRTFSTSFSTFAVLLVIFMLGG
                     ETIRGFVFALMVGVLVGTYSTLFIAVPVSYDISKKKSKAIQEAPATIKLAK"
     gene            6630..>8563
                     /locus_tag="PK641_02630"
     CDS             6630..>8563
                     /locus_tag="PK641_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPG55283.1"
                     /translation="MKIKKLLICIPFLGLFFSLNAQVTTSPTAFIKQDRTEEFDVIFD
                     ASLGNGVMLGETECYAHTGVITTASTGDNDWKHAPVWLDNSDKYKMTKIDESRWSLHI
                     EGGMRQYYNLTEDEVVTKLAFVFRNADGTKEGKTASGNDILYTLYNGDEFAVKITSLN
                     DGSVYEQGETISINAEASESSNMKLTVNGIIKSEISNNLLTYEYTITESGYVPIKVTA
                     EIGEEEVSDSLAIYVKSPVVDEPVPAGMKEGINKTINGDVATITLVLRAPFNNNVYVI
                     GDFNDWSMNEAYQMKRDSVWTKSVLEPSQDSLATVLFWRSFDLPEKSEYYTFQYLVDG
                     SLQISDPYAEIILDPWNDPYIKKKMDSELPAYPTGKGDGLVSVLWGEDPTPYEWQNDF
                     QIADKDNLVIYELHIRDFVSSSDLAGVMSKLDYLEHLGINAIELMPINEFDGNDSWGY
                     NPNHFFATDKAYGTRNEYKEFIDECHRRGIAVILDMVFNHATGINPMAKLYYEGNATS
                     DINPWFNKIAKHPYNVFHDINHEYTGTRQFFKRVLSYWLEEYRVDGFRMDLTKGLTQK
                     DSKDDVGKWGKYDASRIEIIKDYYDTVFATNPNAIFILEHLSEYDEEKVLSDYGMFPW
                     RNMNNSYCQAAMGYAVTAIL"
BASE COUNT         2786 a         1266 c         1719 g         2792 t
ORIGIN      
        1 gttatctacc aaaagaattt gatagaggct gtaaacgaca atgtaaagcg tgtatcagct
       61 gtactttctg tgttttcaat tctgttattg cttatatctt acgccttaat aagcaatact
      121 gttcgtttaa gtgtttatgc aaaacgtttc ttgatacata caatgaaact tgttggtgca
      181 acaagctcat ttatcagacg tccgttttta ctgcaaggtg caattacagg aatattgtcc
      241 gcagttatag caatgggtat gctttcagca attctattct gggctcaatc ggaaatatca
      301 acattactta caacagaaat gttgaacact cttttagttt cattctttgt aatgatattg
      361 tcaggtgtag taattacaat tattgcaaca tatttatctg ttagcagata tttgcgtctc
      421 accattgatg atatgtatta tatttaagag tgtttttttt gttcaataat aataaatttg
      481 agtatgagta ataataatga agataatttg aagtttcgca tagcgcgtaa aaatttatgg
      541 ctaatgggta ttggggtatt gattattgtt gttggattag cattaatgtt gattggaaat
      601 aattcaggac cggatacttt cgagcctgat atattcagtt tccgccggat agttgtagcc
      661 cctatggttg ttttctttgg ctttttgtca attattgtgg caatttgcta ccgtcctaaa
      721 caaaataaga ataatctatg gaatggtatg aagccctagt attaggtttg ctgcaaggct
      781 taacagagtt cttaccggtt agtagcagcg gacatcttga aataggtcaa gtcttatttg
      841 gaacagccgg agaagaaaat ctgacctttg caattgtagt tcatgcagct acagtactaa
      901 gtacaattgt aatactatgg aatgaaatta aattgttgtt taaagggaca tttacaacaa
      961 aagcatggaa tcctgagaaa acatatatag ctaaattaat tgtttctatg attcctgtat
     1021 ttattgtagg cgtatttttt aaggatgcag tagaaagctt ttttggaaat ggtttgttat
     1081 tagtagggat ttgtttatta gtaacagcat tactattagc atttgcttat tttgcaaaac
     1141 ctcgtcagag aaatgaaatt tcatatattg atgcttttat tatcggttta agccaagcag
     1201 tagcagttct accgggtctt tcacgctccg gaacaactat tgcaacaggt ttactattag
     1261 gtaataaaaa agagaacatg gcgcaatttt catttttgat ggttttagtt ccgattttag
     1321 gtgaagcatt tctagaattt atcgatttgg caaaagaagg tgcaccaatg ggcatttctt
     1381 atatgtcttt aataatcgga tttatagccg catttgtatc cggttgtatt gcttgccgct
     1441 tcatgattaa tattgtcaaa aaaggaaaat tgatatattt tgccgtttat tgtgtgattg
     1501 taggtggaat agcaattgca tattctttgt gatatggatt ttaaagaagg cgaaatactc
     1561 tgctttgata aacccttaca ttggacatct tttttgcttg taaacagagt gagatgggcg
     1621 attactaatt atttgggagt aaagaaatta aaagtaggtc atgccgggac cttagaccct
     1681 ttggctaccg gagttatgat attgtgtaca ggtaaagcta caaaaaggat tgacgaattt
     1741 caatatcaaa caaaggagta tgaagcagtt ttgaaattag gtgctacgac gccttcattc
     1801 gatttggaaa aagaaattga tgcgacatat ccaacagagc acattacaga agaactatta
     1861 cggaatgtgt tgtctgattt tgtaggagaa ataagacaag tgccgccggc atactcggcc
     1921 gttaagatta atggcaaacg cgcttacgaa tatgctcgta aaggtgatga tgtagaaata
     1981 aatgcaaaac cacttttgat tgattcaatt gagttattgg aattttcaat gcccattgta
     2041 aaaatcagag ttgtgtgtag taaaggaaca tatattcgtg cattagctcg tgatattggt
     2101 gtagcattgc aaagtggagc gcatcttgta ggtttgcgca gaactagagt cggagatata
     2161 acattagaca aatgcatgtc ttttgaagat tttaaatcaa tgatagatac attgcctgct
     2221 attgtgaatt agttacaatg attttttatt aatattcatt tttatattat gaagttatct
     2281 cagtttaagt tcaaattacc tgaagagttg ttggctcaaa agccatctgc caatagagac
     2341 gaatctcgtc ttttggtatt aaaacgtcaa acaggagaga ttgaacacaa ggtatttaaa
     2401 gacattcttg gatattttga cgagaatgac gtttttgtct ttaatgatac caaagttttt
     2461 ccggctcgtc tatatggaaa taaagaaaaa acaggagcaa gaatagaagt gtttttatta
     2521 cgtgaattaa atcctgattt acgtttgtgg gatgtacttg tagatcctgc tcgtaaaata
     2581 agaataggta ataaattgta tttcggagaa gatgattcat tagtagctga ggttattgat
     2641 aatacaacat ctcgtggtcg tacattgcgt ttcttatttg acggagacca tgatacattc
     2701 aaacaaaccc tatacagtat gggagagact cctcttccac gttttattaa acgccctgtt
     2761 gaacccgaag atacagaacg ttatcagtct atatttgcga aaaatgaagg tgcagttgta
     2821 gcaccggctt caagtttaca ttttagtaga gagttgatga aacgtttgga aattaaaggc
     2881 gtaaactttt ctttcgtaac aatgcatgcc ggtttaggta attttagaga aatagatgta
     2941 gaagatttga ctaaacataa aatggattct gagcagatga ttgttacgga tcaagctgca
     3001 tcagtagtca atgttgcaaa agacgaaaaa cgtcaagttt gtgcagttgg tactactgtt
     3061 atgcgtgccc tcgaaacagc agtatgtact gagggaaaaa tcaaaccatt tgatggttgg
     3121 acaaacaagt ttatatttcc tccatacgac tttacagttg caacatcaat ggtaagtaat
     3181 ttatattctc catattcaca gttacttatg cttgttgcag ctttcggtgg ttatgatcaa
     3241 gtaatgcatg catacgatgt tgcagtaaag gaaaaatacc agtttggtgc ttatggggat
     3301 gcaatgctga ttatataatt atttttcatt gtattttaag aagtagtaag taataacata
     3361 acaagacgcg aaagtttggt cgaatataaa aaatagagta tatttgcacc ctaatttttg
     3421 taaaaatcat ttaatatcaa tcgaatatgc agaacaaggg atttgtaaaa ttgtttgcca
     3481 tcgcattggc gttggtctgc cttttttact tgtcgtttaa tgcagtaaca gcgcattaca
     3541 acagcaaggc agcagaatat gccaatggta acaaaatgaa tgagtatcat tatttggact
     3601 caatggcaac agaaaaggtt tggttaggat ataccttgaa agagtgtcgt gaaaacgaac
     3661 ttaacatggg tcttgactta aaaggcggta tgaatgttat tcttgaagta tccgttccgg
     3721 atattgtaag aacattatca ggcaatacca aagatgaaac ctttaacaag gcaataaatt
     3781 tagctattga acgtcaggca gttagtcaaa aagacttttt ggttttattc caagaggcct
     3841 acgaagaaat cgatcctaat gctcgtcttt ctgcagtatt tactacattc gatttaaaag
     3901 atcgtatatt tttaaaaagt acaaataaag aagttattga tgtcttgaaa caagaagttc
     3961 aggcaactgt agataactcg tttaatgtgt tgcgtactcg tatagaccgt ttcggtgtag
     4021 ttcaaccaaa tatccaacgt cttgaaacca atggacgtat cttaattgaa ttaccgggta
     4081 ttaaagaacc tgaacgtgtt cgtaaattgc tacaaggttc tgcaagtttg gagttctggg
     4141 aaacatataa cttaacagaa atatattctt cattagaact tgcaaatgct gctgttcgtg
     4201 aatacttaaa aggagctgat actacttcag ttgaaaagaa tacagaaaaa gcaaaagcgg
     4261 aagtggcaaa atcagaaaaa gttgttagtg agtctgattc tataaaagtt caattggaaa
     4321 aaattggtgc tgctgctcaa gattcatcaa aaatgataga acaatttgca aaagagaatc
     4381 ctttgttcag tattatgatg ataaatcaaa gtggtggtca attaattcct actccggcta
     4441 tagctatggt tagtattcgt gatacggcaa aagttaatga atacttaaac ttacgtcaag
     4501 taaaagaaat actaccacgc gatttagcat tccgttggac agttaagcca attgatgaaa
     4561 aaggattaat atatcaatta gttgctatta aaataacaaa ccgtgatggt aaagcacctc
     4621 ttggcggtaa cgtaattacg gatgctcgta atgatttgag tcagttctct gcaaactcaa
     4681 cggtaagtat gaccatgaat cctgaaggag caaaaacatg ggctcgttta actaaagaaa
     4741 acatcggccg tagcatagct atcgtgttag ataatatggt ctattcattc ccgaccgtta
     4801 acaccgaaat atcaggaggc cgttctgaaa tttcaggtaa ttttactact gaagaagcaa
     4861 aagacttagc aaatgtattg aagtcaggta aaatgcctgc tccttcgcgt attgtacaag
     4921 aagatgttgt tggaccatct ttgggccatg aagcaattac tcaaggttta tggtctttct
     4981 tgatagcatt tattgttgtt atgctataca tgattttaat gtatggtttg gttccaggtt
     5041 taatagtaga tgtagctttg ttggcaaatg tattcttctt ggtaggtata ttggcctcat
     5101 ttaaagcagt attaacatta cccggcatcg caggtatcgt tttaacaatg ggtatggcag
     5161 tcgatgctaa cgttcttatt tacgaacgta taaaagaaga gttggcagca ggtaaaacgc
     5221 acaaatctgc agtttcagat ggttataaaa atgcattgtc tgcaattatt gactcaaact
     5281 taacaacatt attgaccggt gtcattttgt tctattttgg tacaggccca atcaaaggtt
     5341 ttgcaacgac attgattatc ggtattataa catcattctt tactgctgta tttattacac
     5401 gtgtcattta tgataagatg gttgataatg gctctgtatc taagttaact tttgttacat
     5461 cattctctaa gaacttgttg caaggtgtta atgtaaactg gattaaaatc cgtaagttcg
     5521 gttacatact ttcatgtgca tttgtattaa ttgcaatagt atctctttcg gttcgcggat
     5581 taaatcaggg agttgacttt accggtggtc gtaattatgt agttcgtttc gctgaaaagg
     5641 ttaatacaga agaaattcaa caaatgttag cacccggatt tacagatgct tcagtatcag
     5701 taataactat aggtgatgaa aatcaagttc gtatttctac caactataag attgcagaca
     5761 actctaaaac cattgatgat gaggttgaag acattattta taaatcttta caatcaaaat
     5821 tagctaaaga tggtgtttca aaggaaatgt ttattaaggg ttatattatt gataatggga
     5881 aagctaattt gtctacagat gctaatgttt cgtctttagg tgtgcaaagt tcacaaaaag
     5941 tgggacctgc tattgctgat gacatcaaga atgcagcatt tatggctgta gtgttctcat
     6001 tgcttgtggt tttcttgtat atattggttc gtttcagaaa tgtttcatat agtgttggtg
     6061 cattaagttc attggtacat gatactatat ttattctagg tatttattca ttaacttaca
     6121 ccattatgcc attctcaatg gaaattgatc agtcatttat tgctgcgatt ctaacggtaa
     6181 ttggttattc aattaatgat acagtggtgg tatttgatcg tgtgcgtgaa ctgaagacta
     6241 acttccccaa acgtaatcaa aaagatgtta ttaatgaagc agtaaatgca actttaattc
     6301 gtacatttag tacttcattc tcaacgtttg cagttttgtt ggtaatattt atgttgggtg
     6361 gtgaaacaat ccgcggattt gtattcgcat taatggttgg tgtattagta ggaacatact
     6421 caacattatt tatcgcagtc cctgtttctt atgatatatc aaagaaaaaa tcaaaagcaa
     6481 ttcaagaagc gcctgcaaca ataaaacttg caaaatagta tattatagaa catattttag
     6541 ggagcctgac tttagttggg ctcctttttt tttgttttta ctaaaaaaaa tactatcttg
     6601 caacgttatt tacaaacaaa ccctaatata tgaaaataaa aaaactacta atttgcatcc
     6661 catttttggg attattcttt tctctaaatg cccaagtaac aacttctcca acagctttta
     6721 ttaaacaaga tagaacagaa gagtttgatg taatttttga tgcgtctttg ggaaatggag
     6781 taatgctcgg cgaaacagaa tgttatgcac atacaggtgt aataactaca gcaagtacag
     6841 gagacaatga ttggaagcat gcacctgtat ggttggataa ttctgataaa tataaaatga
     6901 caaaaattga cgaatctagg tggagtttgc acatagaagg cggaatgcgt cagtattata
     6961 atttaaccga agatgaggtt gtaactaagt tagcattcgt atttagaaat gcagacggaa
     7021 ctaaagaggg taagactgcg tctggcaatg acatcttgta tacgttatat aatggtgatg
     7081 agtttgcagt aaaaataact tctttaaatg atgggagcgt atacgagcaa ggtgagacta
     7141 ttagtataaa tgctgaggcg tctgaatctt caaatatgaa gttgacagtc aacggtatta
     7201 ttaagtctga aatttcaaat aatttgttga cctacgaata cacaataacc gaatccggat
     7261 atgttcctat taaggttact gccgaaattg gtgaagagga agtttcagat agtcttgcta
     7321 tatacgttaa atccccggtt gtagacgaac ctgttccagc aggtatgaaa gaaggaataa
     7381 ataaaacaat caacggcgat gttgctacta taacattagt attgcgtgct ccttttaaca
     7441 ataatgtata tgttatcggc gattttaatg attggtcgat gaacgaagct tatcaaatga
     7501 agcgcgatag tgtttggaca aaatcagtat tggaaccatc acaagattca ttggcaaccg
     7561 tattattctg gcgttctttt gatttacctg aaaaatcaga atattatact tttcaatatt
     7621 tagttgatgg atcattacag attagcgatc catacgcaga aatcattctg gatccatgga
     7681 atgacccata tatcaagaaa aaaatggatt cggaattacc tgcatatcca acagggaaag
     7741 gtgatggttt agtttcagtg ctatggggcg aagatcctac tccgtacgaa tggcagaacg
     7801 attttcaaat agcagacaaa gataaccttg tcatatacga attgcatatt agagattttg
     7861 tatcatcaag tgaccttgcc ggtgttatgt ctaaacttga ttatttggaa catttaggta
     7921 taaatgcaat agagttgatg ccgataaacg agtttgatgg aaatgacagt tggggttaca
     7981 atcctaatca tttctttgca acagacaaag catacggaac tcgaaatgaa tataaagaat
     8041 ttatcgatga gtgccacaga cgtggtattg cagttatatt ggatatggta tttaaccatg
     8101 caaccggaat taatccaatg gctaagttgt attatgaagg aaatgcaact tccgatataa
     8161 atccgtggtt taataaaatc gcaaaacacc cttataatgt ctttcatgat ataaaccacg
     8221 agtatacagg tacacgacaa ttcttcaaaa gagttttgtc ttattggtta gaagagtata
     8281 gagttgatgg ctttagaatg gacttgacta aaggtttgac acaaaaagac agtaaagacg
     8341 atgttggaaa atggggtaaa tatgacgcta gtcgtataga aatcataaaa gattattatg
     8401 acacggtatt tgcaacaaat ccaaatgcaa tatttatttt agagcatttg agcgagtacg
     8461 acgaagaaaa agttttatca gattatggta tgttcccttg gcgcaatatg aacaatagtt
     8521 attgtcaagc agctatgggt tacgctgtaa cagcaatttt gtt
//
LOCUS       DAOPID010000044         8504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_10508_length_8504_cov_4.138123, whole genome shotgun sequence.
ACCESSION   DAOPID010000044 DAOPID010000000
VERSION     DAOPID010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 8504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8504
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10508_length_8504_cov_4.138123"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..1353)
                     /gene="purD"
                     /locus_tag="PK641_02635"
     CDS             complement(82..1353)
                     /gene="purD"
                     /locus_tag="PK641_02635"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HPG55284.1"
                     /translation="MNILLLGSGGREHALAWKIAQSPKTNRLYIAPGNAGTASLGENI
                     AINAEDFNAIGDFVRHNNIDMVVVGPEAPLVEGITDFFESEPTLADIAVIGPSKYGAQ
                     LEGSKEFAKKFMMRNNIPTAKYLSVNQNNIEEGKAFLHSLKAPYVLKADGLASGKGVL
                     ILQDIDEAEKELVEMLNGKFGDASATVVIEEFLSGIECSVFALTDGSKYVILPEAKDY
                     KRIGEGDTGLNTGGMGSISPVSFADDVFMKKVEDHIIKPTIKGLHHEKINYKGFVFFG
                     LINVEGEPFVIEYNVRMGDPETESVMLRLQNDIVDLFEATINGKLDKQIIRKDSRYAV
                     SVMLVSGGYPGSYKKGEEVFGLESVTDSVVFHAGTAIKEGRILTNGGRVICVSSYGKT
                     KSEALAKSFENANKISYKGKYFRSDIGFDLK"
     gene            complement(1355..1993)
                     /locus_tag="PK641_02640"
     CDS             complement(1355..1993)
                     /locus_tag="PK641_02640"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPG55285.1"
                     /translation="MKVYTNQFVKMFIEQVPEIVRRFFKPVLWRKDKNKKVLYLTFDD
                     GPNESVTLGVLNELDRLGIKATFFCIGNNVEKHPELFEEIQKRGHKIGSHGYGHIKGI
                     FHDDESFYEDIKKAEKFIQSNLFRPPHGHITPKQIKHICNDYTIVLWDVITRDYNQKL
                     RPEQVYQIAIKYARNGSIIVFHDSVKAAKNMQYAFPKAVEYWQKEGYKFEVL"
     gene            complement(1965..5222)
                     /locus_tag="PK641_02645"
     CDS             complement(1965..5222)
                     /locus_tag="PK641_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HPG55286.1"
                     /translation="MKRFNIIDKLTGLLVFAIAMVTYALTIEPTASFWDCSEFISTAA
                     KLEVGHPPGAPFFMLTGKFFSLFASDTSQIAMMINLMSAFLSALTILFLFWTITHLAR
                     KVIVSNPEQITVAQLIAIIGSGIVGSLVYTFSDTFWFSAVEGEVYAYSSFLTAIAFWL
                     ILKWEDHANEENSDRYLVLICYLMGLSIGVHLLNLLTIPAIVLVYYYKKNENHTWKGI
                     LTALLVSFAILALVLYGMVPGFITLAGYVELLFVNSLGLPFNTGALFYCALTVCVLIW
                     SIFELYKNERKLIRIQISSLLSLTLLGVPFIGGSWIIGLLLIAGAIFYFYFKKDKLNI
                     RMLSTVIVSMAAILIGYSCYSVIVIRSVANPPMDQNSPDNVFSLKNYLNREQYGDRPL
                     VYGPYYSSEPELETVGDYCQVKTKEGEKTWGQKEKTDKSEKDEYIVTGTKYSIIYNRD
                     FQTIFPRMHSNTGDHPKAYESWVNVKGKEVKYDRCGRMVDVKIPTFGENLEFFFSYQL
                     NYMYWRYFLWNFSGRQNDIQGHGEVTNGNWITGFNFIDKYMVGDQTNLPPMYLKNKAH
                     NAYYMLPLILGIIGIVFQIFEKKSGKQSFWVTFVLFFMTGIAIVLYLNQGPSEPRERD
                     YAYAGSFYAFTIWIGFGVAGIAKFLQDRKLPAIMASVIALIISLPVPALMAYENWDDH
                     DRSNRFVARDFGLNYFNSCSKNAIIFTNGDNDTFPLWYTQETEGTEVSLDKRVCNLSY
                     LQTDWYIDQMRRPAYKSEKLPIAWERKTYVEGKRDIVYLDDLIKGPIDLKYALDNFLA
                     SDDPQTKTDPRSGKERYYLPCKKFKVPVNFENLVKQGAVDAKDRDKIASEIIIDLSDK
                     RYLSKSELMILDMIANNNWERPIYYAVTVGSDMYLNLDKYFQLEGLCYRIVPFADTKD
                     NEIVNTDAMYNNMMNKFKFGNIDDQNVYLDENILRMCMAQRQMFGRLIDALIKEDKKD
                     KALKALNYCLDVIPGKTVAHNYSSTSFVEYYYALGEKEKGRALAEEIASDASKNLQWL
                     SQFKGQQRNSAMQDIAINLGALQNLTLISRDYDAEAFDKYYKMFIDYEGIYQSVR"
     gene            5428..5697
                     /gene="rpsO"
                     /locus_tag="PK641_02650"
     CDS             5428..5697
                     /gene="rpsO"
                     /locus_tag="PK641_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPG55287.1"
                     /translation="MQITKKQKAEIFEKYGKSNTDTGSAEGQIALFSARIAHLQEHVL
                     ANHKDYSTKRALVMLVGKRRRLLDYLKDRHIERYRTIIRELGIRK"
     gene            complement(5764..6660)
                     /locus_tag="PK641_02655"
     CDS             complement(5764..6660)
                     /locus_tag="PK641_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HPG55288.1"
                     /translation="MTTKEYTSKIKSAVDRLTSQEFDGRLMHQNHDGEPNPSELSLRC
                     FIDLARTILFPGFFGNTSVSSTTLPYHIGVNVEKLFRVLWEQLFAGMCFECTEEERLL
                     EVKKKNAMNIAAEFVESLPDIKLMLRTDVEAIYKGDPAAKNFGEIILSYPGLKAIVNY
                     RIAHKLLDLGASIIIPRIITEMAHSETGIDIHPGATIGNYFAIDHGTGVVIGETCILG
                     DNVKIYQGVTLGAKSFPTDENGTLIKGIARHPILKNNVVVYAGATILGRITIGENAII
                     GGNTWVTDDVADNTSVYSNVKR"
     gene            complement(6657..8270)
                     /locus_tag="PK641_02660"
     CDS             complement(6657..8270)
                     /locus_tag="PK641_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPG55289.1"
                     /translation="MKRYTLIYIVALLLFSSNTASAQMSIEDQESASTNIRKLANAYW
                     LILSQYVDSVSSEKITENAITSMLDNLDPHSSYLNKEEVKSSNETLQGNFEGIGISYN
                     MIEDTLTVIETISGGPCEKLGVLPGDKIIYVNDSTIAGIKMPTTDIMKRLRGPKGTKV
                     NVKILRQGKWIPFSIIRDKIPIYSIDAAYMLTPKIGYIKINRFAATTYTEMLKAIMKL
                     KSLGMSKLIVDLQSNGGGYMQSAVNIVDEFLPDNKTIVYTEGKNTPKEVLRTQNSNGI
                     FEDGKMVILINEYSASASEILSGAIQDWDRALIVGRRSFGKGLVQRPFTLNDGSEIRL
                     TIARYHTPSGRCIQRPYTGGKEAYEKDMKKRYEHGEFVSADSINLPDSLKYSTLLLKR
                     TVYGGGGIMPDVFVPADTTTMSQLDKEIFNNGIPNTVSLSYTDKHRRELELYNNVKTF
                     ENKFDTTNLLKDIEAELANKKIVYTAKEWEDSKELVSLRTKALVARNIWGIEAYYQIV
                     NKEDNIVKRAIEILSDDNKYEQLLQTNKK"
BASE COUNT         2577 a         1596 c         1309 g         3022 t
ORIGIN      
        1 caatgccggc taatatcgtg aatattaaca gtattagtgg attaatgtta gattgtctgt
       61 cacgttcttg atacatgtct tttatttcaa atcaaatccg atgtcagaac ggaaatactt
      121 tcctttatat gatattttat ttgcattttc aaaagacttt gcaagtgcct ccgattttgt
      181 tttaccatac gaactaacac aaataacacg tccaccgtta gttaatatac gaccttcttt
      241 tattgcagta cctgcgtgaa aaactacact atctgttaca gattctaatc cgaaaacttc
      301 ttcgcctttt ttgtacgagc ccggataacc gcctgaaaca agcatcacac ttacagcata
      361 ccttgagtct ttacgtatta tttgtttgtc aagttttcca ttaattgtag cttcaaataa
      421 atctacgata tcattttgta gtctaagcat tactgattct gtttcaggat ctcccatacg
      481 aacgttgtat tcaataacaa atggttcgcc ttcaacattg attaaaccaa aaaacacaaa
      541 acctttataa ttgatttttt cgtgatgtag tccttttatt gtcggtttga tgatgtgatc
      601 ttcaactttt ttcataaaaa cgtcatctgc aaaacttaca ggtgagattg atcccatacc
      661 tccggtattc agcccggtat caccttcacc aatacgttta taatctttag cctcaggtaa
      721 aatcacatat ttagaaccat cagtcagtgc aaaaacagaa cattcaattc ctgataaaaa
      781 ttcttctatt acaacagttg cactggcatc accgaactta ccattaagca tttcgacaag
      841 ttctttttca gcttcgtcta tatcttgaag aattaaaact cctttacctg acgctaatcc
      901 gtctgctttt aaaacgtatg gagcttttag gctgtgtaag aatgctttac cttcttcaat
      961 attgttttga ttaactgata aatattttgc tgttgggatg ttattccgca tcatgaattt
     1021 ttttgcaaac tccttacttc cttctagttg tgctccgtat tttgatggcc ctataacagc
     1081 tatgtctgct aaagttggtt cggattcaaa aaaatcagtt ataccttcaa caagtggtgc
     1141 ttccggtcca acaacaacca tatcaatgtt gttgtgtctt acaaaatcac caatagcgtt
     1201 aaagtcttcc gcatttattg ctatattttc acctaaagat gctgtaccgg cattacccgg
     1261 tgcaatatac aaacggtttg tttttggact ttgtgctatt ttccatgcaa gggcatgttc
     1321 ccgtcctccc gaacctaata ataaaatatt catattataa aacttcaaat ttatatcctt
     1381 ctttttgcca atattcaact gctttaggaa atgcatattg catattcttt gctgccttaa
     1441 ccgaatcatg aaaaactatt atagatccgt ttctagcata tttaatagca atttgatata
     1501 cctgttcagg ccttagtttc tgattgtaat cacgtgttat aacatcccac aaaacaattg
     1561 tataatcatt acagatgtgt tttatctgtt ttggggttat atgtccatga ggcggtctga
     1621 ataaattact ttgaataaat ttttctgcct tcttgatatc ttcgtaaaac gattcatcat
     1681 catgaaatat tcctttaata tgtccatatc cgtgtgatcc tattttatga cctcgttttt
     1741 gtatctcctc aaacaattca ggatgctttt caacattatt gccaatgcaa aaaaatgttg
     1801 ctttaatacc aagacgatct aattcattaa gtacacccaa tgtaacagat tcgttagggc
     1861 cgtcatcaaa tgtcaaatac aatacttttt tgtttttgtc tttacgccat aaaaccggtt
     1921 taaaaaagcg tctaacaatt tctggtacct gttcaataaa cattttaacg aactgattgg
     1981 tatatacctt cataatcaat gaacatcttg taatatttgt caaatgcttc tgcatcatag
     2041 tctcgactaa ttaaagtcag attctgcaat gcacctaaat ttatggcaat atcttgcatt
     2101 gcgctattac gttgttgtcc tttaaattga gataaccatt gtaaattttt acttgcatca
     2161 gatgctattt cttctgccaa ggctcttcct ttttcttttt ctccaagagc ataataatat
     2221 tcgacaaaac ttgtagaaga ataattgtgt gccaccgttt ttcccggaat aacatctaag
     2281 caatagttca aagctttaag tgctttgtct ttcttatctt ccttaattag agcatcaatt
     2341 agtcttccaa acatttgtct ttgtgccata cacatacgca atatattttc gtctaaataa
     2401 acattttgat catcaatatt accgaactta aatttattca tcatattgtt gtacattgca
     2461 tctgtattta cgatttcgtt atcttttgta tctgcaaaag gaactattct atagcaaaga
     2521 ccctctaatt gaaaatactt atctaagttt aagtacatat cagagccaac ggtaaccgca
     2581 taataaatag ggcgttccca attattatta gctatcatgt ctaaaatcat cagttcactc
     2641 tttgacagat atcttttgtc tgataaatca attataattt cagaagcaat cttgtctcta
     2701 tctttggcat caacggcacc ttgtttaaca agattttcaa aatttacagg aaccttaaat
     2761 ttcttgcatg gtaaatagta tctttctttg ccgctgcgtg gatctgtttt tgtttgtgga
     2821 tcatcgctgg caagaaaatt atccaatgca tattttaagt ctataggacc ttttattaaa
     2881 tcatcgagat atactatatc tcttttgccc tcaacataag tttttctttc ccatgctata
     2941 ggcaattttt ctgatttata tgcaggtcta cgcatctgat ctatgtacca atcagtttga
     3001 agataactta agttacaaac tctcttatct aatgagactt cagtaccttc tgtctcttgg
     3061 gtgtaccaga gtgggaaagt atcattatct ccatttgtaa aaataatagc attctttgag
     3121 cacgaattaa aataatttag gccaaagtct cttgccacaa agcgatttga tctgtcatga
     3181 tcatcccagt tttcgtatgc cattaatgca ggtacgggta gtgaaattat tagtgcgata
     3241 actgatgcca tgattgcagg tagttttcta tcttgtaaaa atttagcaat acctgcaacg
     3301 ccaaatccta tccatattgt aaatgcataa aaggaacctg cgtatgcgta atctctttct
     3361 cgtggttccg atgggccctg attcagatat aaaacaattg ctatacctgt cataaagaac
     3421 agtacaaaag ttacccaaaa agattgtttt ccgcttttct tttcaaatat ctggaatact
     3481 ataccaatga taccaagaat taaaggtaac atgtagtatg cattatgagc tttattcttt
     3541 aaatacattg gaggaagatt cgtttgatct ccaaccatgt acttgtcaat aaaattaaat
     3601 cctgttatcc agtttccatt tgtaacctcg ccatgacctt gaatatcatt ttgtcttcct
     3661 gagaaattcc atagaaaata acgccaatac atgtaattta gttggtaact aaagaaaaac
     3721 tctagatttt ctccaaatgt gggtatctta acatctacca ttcttccaca tctgtcatat
     3781 ttaacttctt tgccctttac atttacccaa ctttcgtaag cctttggatg gtcacctgtg
     3841 ttactatgca ttcgaggaaa aatagtttga aaatcccgat tatatattat tgagtatttt
     3901 gtcccggtaa ctatatattc atctttttcg cttttatcag ttttttcttt ttgtccccaa
     3961 gttttttctc cttcttttgt ctttacttgg caataatcac ctacagtttc caattcgggt
     4021 tcagatgaat aataaggccc gtatactaga ggtctatcac catattgttc acgattaaga
     4081 taatttttta atgagaaaac attatctggt gagttttgat ccataggagg attagcaaca
     4141 gatcttataa cgattactga ataacatgaa tatccgatta atatagctgc cattgaaaca
     4201 ataacagtgc taagcatcct gatgttaagt ttatcctttt taaaatagaa ataaaatatt
     4261 gctccggcta tcaataacaa acctataatc caacttcctc caataaaagg aacgccaagt
     4321 aatgtcaatg aaagtagtga cgaaatttga atccgaataa gctttctttc gttcttgtat
     4381 aattcaaata ttgaccaaat taatacgcat actgttaaag cacagtaaaa tagggcaccg
     4441 gtattgaatg ggagtcctaa tgaatttacg aataacagtt ctacgtagcc tgccagtgtt
     4501 ataaaacccg gaaccattcc gtaaagtaca agtgccaaaa ttgcaaatga tactaataag
     4561 gctgttaaaa tgcctttcca tgtgtggttt tcgttttttt tgtaatagta aactaatact
     4621 attgccggaa tcgtaagtaa attaaggagg tgtacgccta ttgataatcc catcaaatag
     4681 catattaaaa ccagatatct atcagaattt tcttcatttg catgatcttc ccattttaaa
     4741 ataagccaaa atgcaattgc ggtcaaaaat gaagaatatg cgtaaacctc tccttcaact
     4801 gccgagaacc agaaagtatc agagaatgta tataccaaag aacctacaat tccacttcca
     4861 ataattgcaa ttaattgtgc aactgtaatt tgctcagggt ttgatacaat tactttacgt
     4921 gctaaatgtg tgatagtcca aaataagaaa agtatcgtca aagcacttaa aaatgcagac
     4981 attaaattta tcatcattgc aatctgagat gtatcagaag caaataaact aaaaaacttt
     5041 cctgttagca tgaagaatgg tgcgcctgga gggtgtccta cttctaattt tgctgcagtt
     5101 gatataaact cagaacaatc ccagaaactg gcagtcggct ctattgttaa tgcgtatgtt
     5161 accatagcta tcgcaaaaac caataatccc gtcagtttgt cgatgatatt aaatcgtttc
     5221 atgtatattt atattagata aaatccgaac aaactacaaa gatagtttga ataaatgaaa
     5281 aacaaagatt ttgatattgc ctttttacgt tttgtacttc ttatttgaat tttcacttgt
     5341 tgaaactaca caatttgttt tttgtttaaa tattgtgtga ttcaaaaaaa ctttatatct
     5401 ttgccgacct tttcaaatta taaggatatg caaataacaa aaaaacaaaa agcggaaatc
     5461 tttgagaagt acggaaagtc caatactgat actggctcag cagagggtca gatagcatta
     5521 ttctccgccc gtattgctca tttgcaagaa catgtacttg caaatcataa agattatagc
     5581 acaaaacgtg ctttggtaat gttggtagga aaacgtcgta gattactcga ttatttgaaa
     5641 gatcgtcata tcgagcgtta tcgtacaatc attcgtgaac ttggtattcg taaataatcg
     5701 aatagcaaca aaactaaaaa agaggatcgt aagaccctct ttttttatac ttattaattt
     5761 gttttatcgt ttgacatttg aataaaccga agtgttgtct gcaacatcat cagtaaccca
     5821 agtgtttcca ccgataatgg cattttcgcc aatggttatt cttcctaaga ttgttgcccc
     5881 tgcatataca acaacattgt tttttaaaat aggatgtcgt gcaataccct ttattagtgt
     5941 tccattttcg tctgttggaa aactttttgc tcctaaagta acgccttggt atatcttaac
     6001 attgtcacct aatatgcatg tttctccgat aacaacgcct gtgccatggt caatggcaaa
     6061 ataatttcct atcgttgctc cggggtgtat gtcaattccg gtttcagaat gtgccatttc
     6121 cgttatgatt cttggaatta ttattgatgc accaaggtct agtaatttat gtgcaatacg
     6181 atagttaact atagctttaa gtccgggata tgataatatt atttcaccaa agtttttggc
     6241 tgcaggatct cctttatata ttgcttcaac atctgttctt aacattaact ttatatctgg
     6301 aagactttct acgaattctg cagcaatatt cattgcattt tttttcttta cttcaagcaa
     6361 tctctcttcc tctgtgcatt caaagcacat tcctgcaaaa agttgttccc acagtactct
     6421 aaacaatttt tccacattta caccaatatg atatggtaga gtagttgaac ttactgatgt
     6481 attaccaaaa aatcctggga ataagattgt tcttgctaaa tcaatgaaac agcgcagaga
     6541 taattctgaa ggattcggct ctccatcatg attctgatgc attaaacgtc cgtcgaactc
     6601 ttgtgatgta agacggtcta cagctgattt aatcttcgat gtatactctt ttgttgtcat
     6661 tttttatttg tttgcagtaa ttgttcgtat ttattatcat ctgataaaat ctcgatagcc
     6721 cttttaacta tattgtcttc tttatttact atttgataat aagcctcaat accccaaatg
     6781 ttacgtgcta ctaaagcttt tgttcgcaaa gataccaatt ctttggaatc ttcccattct
     6841 ttagctgtat atacaatctt tttattggca agttcggctt ctatatcttt taataaattt
     6901 gttgtgtcaa acttattttc gaatgtcttt acattattat ataattctag ctcgcggcgg
     6961 tgtttgtctg tgtatgacaa actcacggta ttgggtatgc cgttattaaa aatttcttta
     7021 tcaagttgac tcattgttgt ggtatcagca ggtacaaaca cgtcaggcat aatgcctccg
     7081 cctccataaa cagtacgttt taataaaaga gtggagtatt taagtgaatc aggcaaatta
     7141 atactatcag cagatacgaa ttcaccatgc tcgtagcgtt ttttcatatc tttttcgtag
     7201 gcttcttttc ctcccgtata aggtctttgt atgcatctac ctgaaggtgt gtgatatctg
     7261 gcaatagtta gtctgatttc agaaccgtca tttagagtaa aaggcctttg taccaaaccc
     7321 ttaccgaaac ttctacgccc aacaattaag gccctatccc agtcttgtat agcaccggat
     7381 aaaatttcgc ttgcagatgc cgagtattca ttaataagaa taaccatctt tccatcttca
     7441 aatataccat tgctattttg tgtgcgtaat acttcttttg gtgtattttt tccttctgtg
     7501 tacacaatag ttttattatc gggtaaaaac tcgtctacaa tattaacggc actttgcata
     7561 tatccacctc catttgattg taggtcaaca atcagtttgc tcatgccaag actcttcaac
     7621 ttcattattg ctttgagcat ttcggtgtaa gttgttgcgg caaatctgtt aattttaata
     7681 taccctattt taggagtgag catataggct gcgtctatac tatatatagg tatcttgtcg
     7741 cgaataattg aaaaaggaat ccacttccct tgtctaagta tttttacatt tacttttgtg
     7801 cccttaggtc cgcgtagccg tttcattata tcagtagtcg gcatcttaat accagcaatt
     7861 gtcgaatcat ttacataaat aattttatct cccggaagta cgcccaattt ttcgcatggt
     7921 ccgcctgata tggtttcaat aacagttagt gtatcttcta tcatattata ggatatgccg
     7981 ataccctcaa agttaccttg aagtgtttcg tttgaacttt tgacttcctc tttattcaag
     8041 tatgaagaat gcgggtctag attgtccaac atactagtaa tagcattttc tgtgattttt
     8101 tcggacgata cggagtctac atattgactt aggataagcc aatacgcgtt agccaactta
     8161 cgaatatttg tggatgccga ttcttgatct tctatgctca tttgcgcaga tgctgtattg
     8221 ctgctaaata aaagaagtgc tactatatat attaatgtgt atcgtttcat tgtttatttg
     8281 taatattcat ttttgatgct tgtggtttta agcattgcaa agttatgaat ttgtagcgta
     8341 atattgcaat tagtatatta acaaacaaaa aaaacgtaga gtttgtattc aaaaatgcga
     8401 aataatgagt agggatggct ttaaatgcta atcttactta atcttgatat tcagtctatt
     8461 gcaaaaaact ttactatttt cttaaaaaaa gagcaaaaag attt
//
LOCUS       DAOPID010000045         8331 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_10811_length_8331_cov_4.436564, whole genome shotgun sequence.
ACCESSION   DAOPID010000045 DAOPID010000000
VERSION     DAOPID010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 8331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8331
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10811_length_8331_cov_4.436564"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..615
                     /gene="nth"
                     /locus_tag="PK641_02665"
     CDS             7..615
                     /gene="nth"
                     /locus_tag="PK641_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HPG55290.1"
                     /translation="MIDWFDKNMPTAESELHYSNAFELLVAIVLSAQCTDKRVNQTTP
                     ALFSSYPTPQVMADASIEDIQAHIKSISYPNNKAKHLQGLSQVLVKDFNGVVPDTIDE
                     LQKLPGVGRKTANVMLAVYFGKAAMPVDTHVFRLAHRIGLVPASAKTPFEVEQQLSAH
                     IPESLLSKAHHWLLLHGRYVCTARNPKCASCGIKTWCKYITK"
     gene            686..2632
                     /gene="thrS"
                     /locus_tag="PK641_02670"
     CDS             686..2632
                     /gene="thrS"
                     /locus_tag="PK641_02670"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPG55291.1"
                     /translation="MIKITFPDNSVREYESGVTGFQIAESISPRLAQEVLAVSVNGQV
                     TEINRPIITDAAIKLFKWDDPEGKHSFWHTSAHLMAEALQELYPGIQFGIGPAIESGF
                     YYDVDPGEAVIKEIDFPVIEAKVMEIVARKENVIREEISKADALKLFTDKKETYKVEL
                     INDLEDGTITLYHQGNFVDLCKGPHLTNVGEIKAFKITAVAGAYWRGDIKRKQLTRLY
                     AISFPKKKMLDEYVELMEEAKKRDHRKIGRELELFTFSDTVGKGLPLWLPKGTALRLR
                     LEDFLKQIQKRFGYQQIMTPHIGQKQLYVTSGHYAKYGQDSFQPIHTPEEGEEYLLKP
                     MNCPHHCEIYKFKPRSYKDLPLRFAEFGTVYRYEQSGELHGLTRVRSFTQDDAHIFCR
                     PDQIQEEFLKVMDIISIIFNALDFKNFEAQISLRDPNNKEKYIGSDDVWEKAESAIIE
                     ACKSKGLKANVELGEAAFYGPKLDFMVKDAIGRKWQLGTIQVDYNLPERFQLEYVGED
                     NQKHRPVMIHRAPFGSMERFVAVLIEHTGGKFPLWLTPDQVSILPISERFNDYAKKIA
                     ADLEAQDLRVTIDDRNEKIGRKIRDNELKRIPYMIIVGEKEEANGEVSVRKQGEGDKG
                     SMTADAFAKMINDEVREMTISFIK"
     gene            2655..3098
                     /locus_tag="PK641_02675"
     CDS             2655..3098
                     /locus_tag="PK641_02675"
                     /inference="COORDINATES: protein motif:HMM:NF022838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-like domain-containing protein"
                     /protein_id="HPG55292.1"
                     /translation="MKKIMLVLACVMMTIGIQAKDVVTKVTVEELPEKAQKFIKEHFQ
                     AVQVKSVMQETDEDGVIEYTVRLKDKTEIEFDMVGAWNSVLVKKSAMPRFIVPVKVQN
                     AIDKEFANKIIKKVENDGYSFDFDFSDGTEAEINAQGDVTELEIK"
     gene            3205..3765
                     /gene="infC"
                     /locus_tag="PK641_02680"
     CDS             3205..3765
                     /gene="infC"
                     /locus_tag="PK641_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005648683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPG55293.1"
                     /translation="MATNFVPRNNARRVIKENPHRINDFIRGVDEVRLVGENVEPGIY
                     KFKEALRIADEQELDLVEISPSAVPPVCKIIDYSKFLYQLKKKEKEQKAKAAKIVVKE
                     IRFGPQTDDHDYDFKLKHAINFLEEGSKLKAYVFFKGRSILFKEQGEVLLLRFANDLE
                     EYGKVEQLPLLEGKRMIIMISPKKKK"
     gene            3792..3989
                     /gene="rpmI"
                     /locus_tag="PK641_02685"
     CDS             3792..3989
                     /gene="rpmI"
                     /locus_tag="PK641_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPG55294.1"
                     /translation="MPKIKTNSGAKKRFTLTGSGKIKRKHAFKRHILTKKTTKAKRNL
                     TCAALVDHADLSNVKSLLGLK"
     gene            4064..4411
                     /gene="rplT"
                     /locus_tag="PK641_02690"
     CDS             4064..4411
                     /gene="rplT"
                     /locus_tag="PK641_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPG55295.1"
                     /translation="MPRSVNHVASRARRKKVLKLTRGYYGARKNVWTVAKNTWEKGLQ
                     YAYADRKAKKRNFRALWIQRINAAARIEGMSYSKLMGALHKAGIDINRKALADLAMNE
                     PQAFKAIVKKAQA"
     gene            complement(4492..6108)
                     /locus_tag="PK641_02695"
     CDS             complement(4492..6108)
                     /locus_tag="PK641_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPG55296.1"
                     /translation="MITVSNLAIQFGKRILFQDVNLKFTAGNCYGVIGANGAGKSTFL
                     RMLSADLDPTRGTISFGHNERLSVLKQDHFEFDEFTVIDTVMMGHDRLWKVKEEKDII
                     YAKEDFSEADGIKASELEEAFAEMDGWNAESDAASLLSSLGIKETNHYTLMKDMSGKE
                     KVRILLARALFGKPDNLLLDEPTNDLDIETIRWLEDYLANYENTVLIVSHDRHFLDAV
                     STHIIDIDYGKIQMFTGNYSFWYESSQLALRQQQTQNKKAEEKKKELEEFIRRFSANV
                     AKSKQATSRRKMLDKLNIEEIKPSTRKYPGIIFTPEREPGNMVLEVKGLSKTVDGTVL
                     FKDVNFNIEKDDKIAFVSRDPRAMTALFEIINGNIKADSGSFNWGVTITHAYLPLDNS
                     KFFNTDLNLVEWLGQFSNDTTESFIRGYLGRMLFAGEEIFKKASVLSGGEKMRCMISR
                     IMLKNANVMVLDSPANHLDLESIQAFNNSLKTFKGNVLLSSHDHEFISTVCNRIIELT
                     PNGIIDKVMDYDDYLDDANVKAQQIKLYGM"
     gene            6296..7900
                     /locus_tag="PK641_02700"
     CDS             6296..7900
                     /locus_tag="PK641_02700"
                     /inference="COORDINATES: protein motif:HMM:NF025499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HPG55297.1"
                     /translation="MMNVFTEEDKQYIKSLGHTLESVEEQLSIFKKGVPVPNFLRTAT
                     IDDGIQIYTNAEIDAYAASWDSYLEKGKPIAHFIPASGFAGRFFRDLINFLNAPYDEP
                     QTNFEKNFFKHLPSFAFFGELNKFCLATEKEGLQELLKDGKYKLIVEYILTEKGLNFC
                     SLPSALFKFHTDKSHRFVVIQKYLPKKIVKYYGSFEDTRTPIQESLIESAMVSGVKGG
                     TVNVCFTVRKEHKEVIQDYVREFKYPIEKKLGLSFFVTLPCQKTNTDTIVVTPDNLPF
                     RDANGNIMFQQAGHGALLQNLNSLPADVVFIKNIDNEAPDSLKKLTSHYKKALGGILL
                     ANQKIISRFLRLFDKDEMPDDKLIEAINYVENTLNVKRDNILRLTRQEQVDYLKSKLN
                     RPLRVCGMVKNEEEQGGMPCWVKNDDGTISLQIIEYYQIYDNPTLMNLFNSSTHFNPV
                     DIVCSMVDYKGKKIDLTQYADKKSAMVYNKNIEGQAVKVMELPGLWNGGMADWNTIFV
                     EVPIKTFNPVKTVNDLLRHEHQNTNK"
     gene            7936..>8331
                     /locus_tag="PK641_02705"
     CDS             7936..>8331
                     /locus_tag="PK641_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013063337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan-branching enzyme"
                     /protein_id="HPG55298.1"
                     /translation="MENLKIIQHDPWLEPFKSAIEGRYQYALSKGNELTQDHRLSLSD
                     FASGYLYFGMHKTSDGWVVREWAPNATDIFLIGDFNQWQRNEAYKLKRLSNGVFEGIF
                     PEKSIQHGQLYKFYICWNGGAGERIPAWCR"
BASE COUNT         2887 a         1337 c         1552 g         2555 t
ORIGIN      
        1 tcatatatca tagattggtt tgataagaat atgcctacag ccgaatctga gttgcattat
       61 tcaaatgctt ttgaattgtt ggtggccata gttctgtctg cacagtgtac cgataaacgt
      121 gttaatcaaa caactccggc tttattttca tcttatccta cgcctcaagt tatggccgat
      181 gcttcaattg aagacataca agctcatatc aaaagtatat catatcctaa taataaagca
      241 aaacatttgc aaggtttgtc gcaagtactg gtaaaagatt ttaatggagt agtacctgat
      301 acaatagatg aacttcaaaa attacccggt gtagggcgta aaacagctaa tgttatgctt
      361 gcagtatatt ttgggaaagc agcaatgcct gtagatactc atgtgttcag gcttgcacac
      421 cgtatagggc ttgtaccggc atcagccaaa acaccatttg aagttgaaca acaattatcg
      481 gcacatattc ccgaatcatt gttaagtaaa gctcatcatt ggttattatt gcatggtcga
      541 tatgtatgta cggctcgaaa tccaaagtgc gcaagttgtg gaataaaaac atggtgtaaa
      601 tacattacta aataagacga atatttttca ataaattatt aactttgcat tccttattaa
      661 tagtaagata taaagagttc attatatgat taaaattaca tttccggata attctgttcg
      721 cgagtacgaa agcggcgtaa cgggattcca aattgcagaa agtattagcc cgcgcttggc
      781 acaggaagtt ttagctgtgt ctgttaacgg acaagtaacc gaaatcaatc gcccaataat
      841 aacagatgct gctattaaat tatttaaatg ggatgatcct gaaggaaaac attcattctg
      901 gcatacatct gctcacttaa tggctgaagc attacaagaa ttgtatcccg gaattcaatt
      961 cggaatagga cctgctatag aaagtggttt ctactatgat gttgacccgg gtgaagcagt
     1021 aattaaagaa attgattttc ctgtaataga ggcaaaagta atggaaatag tagcgcgtaa
     1081 agaaaacgta attcgcgaag aaatttctaa agcagatgct ttaaaattgt ttaccgataa
     1141 aaaagaaaca tacaaagtcg aattaatcaa cgatttggaa gatggtacaa ttacattgta
     1201 tcatcaaggt aatttcgttg atttgtgtaa aggaccacac ttaacaaatg taggcgaaat
     1261 caaagcattt aagattactg cagtagccgg tgcatattgg cgcggagata ttaaacgcaa
     1321 acaacttaca cgtttatatg caatctcatt ccctaaaaag aaaatgcttg atgagtacgt
     1381 tgaattgatg gaagaagcaa agaaacgtga tcaccgtaaa ataggaagag aactggaatt
     1441 atttacattc agcgatacag taggaaaagg tcttcctttg tggttaccca aaggaacagc
     1501 attgcgttta cgtcttgaag atttcttgaa acaaattcaa aaacgcttcg gttaccaaca
     1561 aataatgact cctcatatcg gtcaaaaaca attatatgtt acttctgggc actatgctaa
     1621 atatggtcaa gattcattcc aaccgataca cactcccgaa gaaggtgaag agtatttgtt
     1681 gaaacccatg aactgccctc accactgcga aatatataaa ttcaaaccac gttcttataa
     1741 agatcttcct ttgcgttttg cagagttcgg aacagtatat cgttatgagc aaagtggtga
     1801 attacatggt ttaaccagag tacgtagttt tactcaagat gatgctcaca tattttgccg
     1861 tcctgatcaa attcaagaag aattcttgaa agtaatggat attatttcga taatatttaa
     1921 tgctcttgat ttcaaaaatt tcgaagcaca gatttctcta agagatccga ataacaaaga
     1981 gaaatacata ggtagtgacg atgtatggga aaaagcagaa tcagccatta ttgaagcatg
     2041 taaatcaaaa ggattaaaag caaatgtcga attaggcgag gcagctttct atggtcctaa
     2101 attggacttt atggtaaaag atgcgatagg tcgtaaatgg caattaggca ctatacaggt
     2161 agattataac ttgcctgaac gtttccaatt agaatatgtc ggcgaagata atcaaaagca
     2221 tcgtcctgta atgattcaca gagcaccatt cggttcaatg gaacgttttg ttgctgtatt
     2281 aattgaacat acaggcggta aattcccatt gtggttaact cctgatcaag tttcaatact
     2341 tcctattagc gaacgtttta acgattatgc taaaaaaatt gcagcagatt tagaagcaca
     2401 agatttacgt gttaccatag atgacagaaa tgagaaaatt ggccgcaaga ttcgtgataa
     2461 tgaattaaaa cgtattcctt acatgattat tgtaggtgag aaagaagaag caaacggtga
     2521 agtatctgta cgtaaacaag gtgaaggtga taaaggcagt atgactgctg atgcatttgc
     2581 aaaaatgatt aatgatgaag ttcgtgagat gactattagt tttataaaat aacgaattaa
     2641 taacaaaaag ccttatgaaa aagattatgt tagttttggc ttgcgtgatg atgacaatag
     2701 gcatccaagc caaagatgtt gtaacaaaag ttactgttga agaattgccc gaaaaagcac
     2761 aaaagtttat taaagaacat tttcaggctg ttcaagttaa atcggtaatg caagaaacag
     2821 atgaagacgg tgtcattgaa tacacagttc gtctgaaaga taaaacagaa atcgagtttg
     2881 atatggttgg agcttggaat tctgtacttg ttaagaaatc cgcaatgcct cgctttattg
     2941 ttcctgtaaa ggttcaaaat gctattgaca aagaatttgc aaataaaata atcaaaaaag
     3001 ttgagaatga tggatatagc tttgattttg atttttcaga cggaaccgag gctgaaatta
     3061 atgcacaagg agatgtaacg gagttagaaa ttaaataaat ttccttttgt gttgtgtatt
     3121 tattaaaatt ggcgtatatt tgcactcttg tttttgaaag gataatttaa taattaaatt
     3181 agaacgttta ggaggacgta gcttatagca acaaattttg taccgagaaa caatgccaga
     3241 cgtgttataa aagaaaaccc gcatagaatt aatgatttta tcagaggggt ggatgaggtt
     3301 cgtttggttg gtgaaaatgt agagcccggt atctacaaat ttaaagaagc attacgaatt
     3361 gcagatgagc aagagcttga tttagtagaa atatccccat cagcagttcc tcctgtttgt
     3421 aagattattg attattcaaa atttctttat caactaaaga aaaaagaaaa agaacaaaaa
     3481 gcaaaagcag ccaagatagt cgtaaaagag attcgttttg gtcctcagac tgatgatcac
     3541 gattacgatt ttaaattaaa acacgcaatc aatttccttg aagaaggatc aaaattaaag
     3601 gcgtatgtat tttttaaagg tcgttcaatc ttatttaaag aacaaggcga agtattgtta
     3661 ctacgttttg ccaacgattt ggaagaatac ggaaaagtag aacagttgcc attattagaa
     3721 ggaaagagaa tgattataat gatttctcct aaaaagaaga agtaattttt atttatttaa
     3781 tatataacca aatgccaaaa attaaaacta attccggtgc caaaaaaagg tttaccctta
     3841 ccggatcagg aaagatcaag agaaagcatg cttttaaacg ccatatcttg acaaagaaaa
     3901 cgaccaaagc aaaacgtaat cttacgtgtg cagcgttggt agatcatgca gatttatcta
     3961 acgtaaaatc gttattgggt cttaaataaa aatagttatt aaccgaatgt ttagcaaaaa
     4021 agatcacaaa atgtgacgct aacaatcaaa aaacacaaag aacatgccaa gatcagtcaa
     4081 ccatgttgct tcaagagcaa gaagaaaaaa ggttttaaaa ttaacccgcg gttattatgg
     4141 tgcccgcaaa aatgtttgga cagtagcaaa aaatacttgg gaaaaaggtt tgcaatatgc
     4201 ttatgcagat cgtaaagcta agaaacgtaa tttcagggct ttatggattc aacgtattaa
     4261 cgctgcagca cgtattgaag gaatgtctta ttctaagttg atgggtgctt tacacaaagc
     4321 aggtatagac attaatcgta aagcattggc agatttagca atgaacgagc cacaagcatt
     4381 taaagcgatt gtaaaaaaag cacaagctta agtattaagc gacattataa aaaaatagga
     4441 cactaaacag tgtcctattt tttttatgcc atatattata tggaatgcta tttacatccc
     4501 atataatttt atttgttgag ccttaacgtt agcatcatct aaataatcat cgtaatccat
     4561 tactttatca ataatgccat taggagttaa ttctataata cggttacaaa ccgtacttat
     4621 aaattcgtgg tcatgcgatg ataacaatac gttgccctta aatgtcttaa gcgaattgtt
     4681 gaatgcttga attgactcaa gatctaagtg gttggcagga gagtcaagta ccataacatt
     4741 ggcattcttt agcataattc gcgaaatcat gcaacgcatt ttttcaccac cggataatac
     4801 acttgctttt ttgaatatct cttctccggc aaataacatt ctacctaaat atccccgaat
     4861 aaatgattct gtagtatcat tagaaaattg tcctaaccat tctaccaaat ttaaatcggt
     4921 attaaaaaac ttagaattgt caagcggtaa atatgcgtgt gttatagtaa caccccagtt
     4981 aaaacttccg gaatcggctt taatatttcc attaataatt tcaaataaag cggtcattgc
     5041 acgaggatcg cgtgatacaa atgcaatttt atcgtctttt tcaatattga aatttacatc
     5101 tttgaataat actgtaccat caacagtttt agataagccc ttaacttcca gaaccatatt
     5161 ccccggctct ctttcgggag tgaaaataat ccccggatat ttacgagtag aaggcttgat
     5221 ttcttcaata ttcagtttat ccaacatttt gcgtctgctt gtagcttgtt tagattttgc
     5281 aacattggca ctaaaacggc gaataaactc ttccaattct ttcttcttct cttcggcttt
     5341 cttgttttgc gtttgttgtt gacgcaatgc taactggcta gattcatacc agaaagaata
     5401 attaccggta aacatctgta ttttgccgta gtcaatatct atgatatgtg tactaacagc
     5461 atcaaggaag tgtctgtcgt gtgatactat aagaactgta ttttcgtaat ttgctaagta
     5521 atcctccagc catctgatag tttctatatc caagtcgtta gtaggctcat ctaataatag
     5581 attatcaggt tttccaaata atgcacgtgc taataaaata cgaacttttt ctttaccgct
     5641 catatctttc attagagtgt agtgattagt ttcttttata cctaagctac tcaataatga
     5701 tgctgcatcg ctttcagcat tccatccgtc catttcggca aatgcttctt ctaattcgga
     5761 tgcttttatg ccgtcagcct cgctaaaatc ttcttttgcg taaatgatat ctttttcttc
     5821 tttgactttc cacagacgat cgtggcccat cataacggta tcaataaccg taaattcatc
     5881 aaactcaaag tggtcttgtt ttaatacaga aagtctttcg ttatgtccaa aagaaatagt
     5941 tcctcttgta ggatccaggt cggcacttag catacggagg aaggttgatt ttccggctcc
     6001 gttagctcca atcacaccat agcaatttcc tgcagtaaat ttgagattaa catcttgaaa
     6061 taagatgcgt ttaccgaatt gaatcgctaa attggaaaca gtaatcatat cgcttgtata
     6121 taaattttaa taagagcgca aaggtactca ttttaaacga atcgggcaaa taagtaagtt
     6181 gtctgcttgt aataaaagta tacaattgaa ttaattgtaa gctaatctga aaataaggtg
     6241 tttttatttg aaaaatcggc taaaaaaact tatttttgta cttctaatgt attaaatgat
     6301 gaacgtattt acagaagaag ataagcaata tatcaaaagc ctcggccata cacttgaatc
     6361 agttgaagaa cagttatcaa tatttaagaa aggtgtaccg gtgccaaatt ttttacgtac
     6421 tgcaactatc gatgatggta ttcaaatata tacaaatgca gagatagacg cttatgctgc
     6481 gtcatgggat tcgtatcttg aaaaaggtaa accgattgct cattttattc ccgcctcagg
     6541 ttttgcagga cgtttctttc gtgatttgat aaattttctt aatgctccat acgatgagcc
     6601 tcaaaccaat tttgaaaaga atttctttaa acatttgcct agttttgcat tttttggaga
     6661 attaaataaa ttttgtttag ctacagaaaa agaaggcttg caagaattac tcaaagatgg
     6721 taaatataag ctgattgtag aatatatttt gacagaaaaa ggactcaatt tttgcagttt
     6781 accaagtgca ttatttaagt ttcacacaga taaatcgcat cgttttgttg taattcaaaa
     6841 atacttacca aagaagattg taaagtacta tggctctttc gaagatactc gtacacctat
     6901 tcaagaaagt ttgattgaaa gtgcaatggt atcaggtgta aaaggaggga ctgtaaatgt
     6961 atgctttacg gtaagaaaag agcataaaga agttattcag gattatgtac gtgagtttaa
     7021 gtatcccatt gaaaagaaat taggcttatc attttttgtt acattaccct gtcaaaaaac
     7081 aaatactgat acgattgtcg ttacgcctga taatttacca tttagagatg caaatggcaa
     7141 tattatgttt caacaagcag gtcatggcgc attgctccaa aatttaaata gtttaccggc
     7201 agatgtggta tttattaaga atatcgataa cgaggctcct gattcgctca aaaagctgac
     7261 atctcattat aagaaagcct taggtggtat tttattggcg aatcaaaaaa ttattagtcg
     7321 tttcttacgt ttgtttgata aggatgaaat gcctgatgat aaattgattg aagctattaa
     7381 ttatgtcgaa aatactttaa acgtaaaacg agacaatatt ttacgtttaa cacgtcaaga
     7441 acaagttgat tatttaaaaa gcaaattaaa tcgtccgcta cgtgtgtgcg gtatggttaa
     7501 aaacgaagaa gaacaaggtg gtatgccgtg ctgggttaaa aacgacgatg gtactatttc
     7561 tttgcaaatc atagaatatt atcagatata tgataatcct actttgatga acttgtttaa
     7621 ttcgtcaact cactttaatc ccgttgacat agtttgttcg atggttgatt ataagggcaa
     7681 aaaaattgat ttaacacagt atgctgataa aaagtcagct atggtatata ataagaatat
     7741 tgaagggcaa gcggttaaag taatggaact tcccggatta tggaatggcg gtatggccga
     7801 ttggaatacg atctttgtag aagtacctat aaagacattt aatcctgtaa aaacagtaaa
     7861 tgatttgtta cgtcacgaac atcaaaacac aaataaatag accacactaa ttactctcca
     7921 aaaaacccaa acattatgga aaatttaaag atcatacaac atgatccatg gttggaaccc
     7981 tttaagtcag ctattgaagg tcgttatcaa tatgcattga gtaaaggtaa cgaactaaca
     8041 caagaccata gattaagtct gtcagatttt gcttccggat atctatattt tggaatgcat
     8101 aaaacatcag acggatgggt cgttagagaa tgggctccaa atgcaactga catattcttg
     8161 attggagatt ttaaccaatg gcaacgtaat gaagcatata agcttaaacg tctttcaaat
     8221 ggtgtttttg agggtatttt ccctgaaaaa agtattcaac acggccagtt atataagttt
     8281 tacatttgtt ggaatggagg agcaggtgag cgaatacctg cttggtgtag a
//
LOCUS       DAOPID010000046         8190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_11058_length_8190_cov_5.683467, whole genome shotgun sequence.
ACCESSION   DAOPID010000046 DAOPID010000000
VERSION     DAOPID010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 8190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8190
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11058_length_8190_cov_5.683467"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..1414)
                     /locus_tag="PK641_02710"
     CDS             complement(164..1414)
                     /locus_tag="PK641_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HPG55299.1"
                     /translation="MNTILRIGICNRPSISVNFHGDFTDKNGKIYNGKHTFSESIFLS
                     PKTVDSIFELYDITIGINFHWQRNENQCFTGALECKLNEGMLIAINHIELEDYLHSVI
                     ASEMNAKASLNLLKAHAVISRSWALAQVNKELSEINGSDYPEDTENKHIKWYGHENHK
                     LFDLCADDHCQRYQGITRINSEVVKQAVKETEGEILVSDNRICDTRFYKCCGGLTELY
                     ENTWDEHHHPYLISLTDSNTPNTNDLSIEQNAVDFIESEPDAFCNTKDKTILSQVLND
                     YDLETNDFYRWKVEYTQQELSSIVKERSGIDFGNIQDLIPIKRGASGRIIELKIIGTK
                     KTMIIGKELEIRKYLSHNHLYSSAFIVKRTPDNGFVLHGAGWGHGVGLCQIGAAVMAD
                     KGYNYRQILAHYYPNSEIKQINIY"
     gene            complement(1411..2223)
                     /locus_tag="PK641_02715"
     CDS             complement(1411..2223)
                     /locus_tag="PK641_02715"
                     /inference="COORDINATES: protein motif:HMM:NF027594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HPG55300.1"
                     /translation="MGNSDLYLFFDKQISKWPLAAANYKKLEQVRTKDIQFDGFSFKV
                     LYNPARAVSSFAKLDPSAIATRPCFLCKKNRPDEQISINYKDKYDILVNPYPICPIHF
                     VVAAKNHEEQSIAGRFEDFTELAQLFPDFVVLFNGAKAGASAPDHFHFQLANKSFFQN
                     DIDASMPGLIKKDSFKSTDSTAVQKWLHDYFTEFDEANANIFCEYENGKWNATIFPRI
                     KHRPNQFFTGEILISPGAIDMTGNLIVAREEDFAKINKEVLTDVLKQISGKI"
     gene            complement(2326..2628)
                     /locus_tag="PK641_02720"
     CDS             complement(2326..2628)
                     /locus_tag="PK641_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPG55301.1"
                     /translation="MNIFVGSLSFSVKDDELKAVFEQYGEVSSARVLTDKFTGRSKGF
                     GFVEMPDDAAAQKAVDALNGTEIGGRAVVVNQARERSEEPRRTGGYQKRDNGFKRY"
     gene            2962..3852
                     /gene="era"
                     /locus_tag="PK641_02725"
     CDS             2962..3852
                     /gene="era"
                     /locus_tag="PK641_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HPG55302.1"
                     /translation="MHKAGFVNIIGNPNVGKSTLMNALVGERISIITSKSQTTRHRIM
                     GIVNGDDFQIVYSDTPGVLKPHYRLQESMLEASQSALSDADVLLYVTDTFETYDKNSE
                     FLEKTKRSGVPTFVIINKIDLLPSQEALENLVEKWKSVLPDAIIIPVCALERFNLDQL
                     LKQILALLPESPPYFDKDALTDRPARFFVTEIIREKILETYDKEVPYSTEVVVEKFQE
                     DEKLIRINAMIIVERDSQKGIIIGKGGSMLKKVGMAARTEIEAFLEKKVFLELYVKVE
                     KDWRNKDKQLKSFGYNIVQP"
     gene            3891..5216
                     /gene="der"
                     /locus_tag="PK641_02730"
     CDS             3891..5216
                     /gene="der"
                     /locus_tag="PK641_02730"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPG55303.1"
                     /translation="MSIVAIVGRPNVGKSTLFNRITETRRAIVSDEAGTTRDRQYGKA
                     EWGKKEFSLIDTGGLVANSGDMFEKEIKKQVNIAIDEADVILFLVDVQTGISELDMDV
                     AAILRACKKPVLLVANKTDENEFQYQAAEFYRLGIGDPYCVSAATGFGTGDLLDAIVP
                     LLSEREIEYVDDSLPRIAIVGRPNAGKSSLLNSLMDEERNIVTDVAGTTRDSIYTKYN
                     KYGKEFYLVDTAGIRKKAKVNEDLEFYSVMRAIRTIENSDVCIMMIDATRGIESQDQN
                     IFSLIQKNKKGLVVCVNKWDLVENKEQKAITTFENAIRERFAPFVDFPIVFTSATTKQ
                     RIFKVIDVALEVYGNKKRRIGTNKLNEFILPLIENYPPPALKGKYIKIKYVTQLPDTY
                     VPTFVFFANLPQYIKDPYKRFLENKIRETWDFTGIPLQLFFRAKSTGRK"
     gene            complement(5289..5732)
                     /gene="rplI"
                     /locus_tag="PK641_02735"
     CDS             complement(5289..5732)
                     /gene="rplI"
                     /locus_tag="PK641_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HPG55304.1"
                     /translation="MKIILKEDVQGLGYKDDVLTVKSGYGRNFLIPTGKAVLATESAQ
                     KVLAENLKQRAHKLAKIKADAEELAAKLNGLTISLGTKASSTGKVFGSVTSLQIAEAL
                     DKQGLKVDKRTIVLKDAIKELGSFKVNVKLHKEVSAEITIEVVAE"
     gene            complement(5752..6021)
                     /gene="rpsR"
                     /locus_tag="PK641_02740"
     CDS             complement(5752..6021)
                     /gene="rpsR"
                     /locus_tag="PK641_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPG55305.1"
                     /translation="MAQNQSEIRYLTPQSVDTKKKKYCRFKRSGIKYIDYKDPEFLKK
                     FLNEQGKILPRRLTGTSLKYQRMVAQAIKRARHLALLPYVTDMMK"
     gene            complement(6042..6434)
                     /gene="rpsF"
                     /locus_tag="PK641_02745"
     CDS             complement(6042..6434)
                     /gene="rpsF"
                     /locus_tag="PK641_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPG55306.1"
                     /translation="MNHYETVFILTPVLSDVQMKEAVDKFKTVLKSNNAEILNEENWG
                     LRKLAYPIEKKSTGFYQLLEFTAEPEIVNTLETQFRRDERVIRFLTTKMEKYAIEYSE
                     KRRKKTNAPAEDKAAKPVKKAAEASNNE"
     gene            6733..7308
                     /locus_tag="PK641_02750"
     CDS             6733..7308
                     /locus_tag="PK641_02750"
                     /inference="COORDINATES: protein motif:HMM:NF014639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HPG55307.1"
                     /translation="MQTSKLLYFGYCANIFVLTGVLCGSLYIQLVLNEFPCPLCMLQR
                     MAMILCALSLVYMLSKTLYYGYPNASAVSVSNGLNIVSSLVGASISIRQILLHIMPSD
                     PGYGDAILGFHIYTWALIVFICEIAFSAGVMLLNPNHWDKLPLSWHKPIKVLFYWLAI
                     VVVIFLLATFNEEGFNWVLPDNPTENAFINF"
     gene            complement(7321..7791)
                     /locus_tag="PK641_02755"
     CDS             complement(7321..7791)
                     /locus_tag="PK641_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HPG55308.1"
                     /translation="MGNLDKTDLLILQQLQKDSQLTTKELALKVHLSPTPVFERLRRL
                     EREGYIKGYQAILDAEKLNKGFMAFCYLKMKQHSHENATAIMSAVQNIPEIVECYNIS
                     GDYDFLLKIYAENMKQYQSFVLRILGDLDCIGSLNSSFVLGEVKNSHQLPIDIE"
     gene            8039..>8190
                     /locus_tag="PK641_02760"
     CDS             8039..>8190
                     /locus_tag="PK641_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HPG55309.1"
                     /translation="MGTKKLHFETLQIHVGQEKADPFTDSRAVPIYQTTSYVFHNSAH
                     AAARFN"
BASE COUNT         2559 a         1317 c         1410 g         2904 t
ORIGIN      
        1 acgatatccg gctttatcgg caagaatact tacaaagaat aaagtagata tggttagtag
       61 gattaattcg atttgcattg tgcatttttt tgttaattcg caaaattagt aaaaaaagag
      121 tatattcttt taagttaaaa ctcaaaataa gatagaatgt tttttaatat atgtttattt
      181 gtttgatttc ggaattagga taataatgtg caagaatttg tcgatagtta tatcctttat
      241 cagccataac agcagcacca atctggcata aaccaacgcc atgtccccaa cctgcaccat
      301 gtaatacaaa tccattatcg ggtgtacgtt taactataaa agcagaacta tataaatgat
      361 tgtgtgataa gtatttacgt atttctaatt ccttaccgat tatcattgtt ttttttgttc
      421 ctataatttt aagttcaata attctgccgg atgctcctct ttttattgga attagatctt
      481 gtatgtttcc aaaatcaata ccggaacgtt ctttcacaat actcgataat tcttgttgag
      541 tgtattcaac tttccatctg taaaaatcat tagtttctag gtcatagtcg ttcaaaacct
      601 gagatagaat agttttatcc tttgtgttac agaaagcatc aggctctgat tcaataaaat
      661 caacagcatt ttgttcaata cttaaatcat ttgtgtttgg agtattgcta tcggtcaaac
      721 taataagata tgggtgatga tgctcgtccc atgtattttc atataattca gtaagccctc
      781 cacagcattt ataaaaacga gtatcacata tacggttatc tgagaccaaa atttcacctt
      841 cagtttcttt aactgcttgc ttaacaactt cagaattaat tcttgtaatt ccttgatatc
      901 tttgacaatg gtcgtctgca cataagtcaa ataatttgtg gttttcgtgt ccgtaccatt
      961 tgatatgctt attttcagta tcttcaggat aatctgaacc atttatttca gatagttctt
     1021 tattaacctg cgccaatgcc caactgcgcg aaataacggc atgggctttt aataaattga
     1081 gtgatgcttt tgcattcatt tccgaagcaa ttacactatg taagtagtcc tccaactcaa
     1141 tatgattgat ggcgattaac atgccttcat tcaatttaca ttctagtgca ccggtaaagc
     1201 attgattttc atttcgttgc caatgaaagt ttattccaat agtaatgtca tataattcaa
     1261 atatggaatc tacggtttta ggacttagaa atattgactc agaaaatgta tgtttaccat
     1321 tatatatttt accattttta tctgtaaagt ctccatgaaa atttacagat atgcttgggc
     1381 ggttacaaat acctatacgt agaattgtat tcatatttta cctgatatct gcttaagtac
     1441 atcagtcaaa acttctttat tgatttttgc aaaatcttct tctcttgcaa ctatcaaatt
     1501 gccggtcatg tcaattgctc cggggctgat taatatttca ccggtaaaaa attgattggg
     1561 tctatgtttg atgcgcggaa atattgtggc attccatttc ccgttttcat attcacaaaa
     1621 gatatttgca tttgcttcgt caaattcagt aaaataatcg tgcaaccatt tttgtacagc
     1681 agttgaatcg gtcgatttaa aactatcttt tttgattaaa ccgggcatcg atgcatcaat
     1741 atcattttga aagaaacttt tgttagccag ttgaaagtga aaatggtcag gagctgaagc
     1801 gcctgcttta gctccgttga acaatactac aaaatcagga aataattgtg ctaattctgt
     1861 aaaatcctca aaacgtcctg caatagattg ttcttcgtga tttttagcag cgaccacaaa
     1921 atgaataggg cagataggat atgggtttac taaaatatcg tatttatctt tgtaattaat
     1981 gcttatttgc tcgtcaggtc tatttttttt gcataaaaag catggtcgtg ttgcaatggc
     2041 tgacggatct aattttgcaa atgatgatac agctctggcg ggattgtata atactttaaa
     2101 tgagaaacca tcaaactgaa tatctttggt acgtacctgt tctaattttt tataattggc
     2161 tgcagctaac ggccattttg atatttgttt atcgaaaaac aaatacaaat ctgaatttcc
     2221 catatacata gtttttgttc tgcacaaaat taataaaaca caaacaaaaa aagcggtttt
     2281 acttatgtaa aaacgctttt taaagttcaa cggatttgaa atatatcaat atcttttaaa
     2341 accattgtct cttttttgat aaccgccagt acgtcttggt tcttctgagc gctcgcgagc
     2401 ttgattaaca acaactgcac gaccaccaat ctctgttccg tttaatgcat caactgcttt
     2461 ttgtgctgct gcatcatcag gcatttcaac aaaaccaaaa cctttgctac gtccggtaaa
     2521 tttgtctgtg agtacacggg cagaagaaac ttctccatat tgctcaaaaa ctgcttttaa
     2581 ttcgtcgtct tttactgaaa aagatagact tccaacaaaa atgttcatta aatattaaaa
     2641 taaaattgtt taataattcg ccaaaggtac gccttttttt tacacagtcg tcattttttt
     2701 ataaaaataa tgcgtttttt gtttaaataa gtgattttgc ctgttttagc atatctgtaa
     2761 ggcttttgtt attagttgtt ttttagctgt attttggtgt ttttaccggc gttttttagg
     2821 tttttattta cttttttaga taaaaatgaa gtatgaataa tacattcatt taagtttttt
     2881 tttgaatttg ctttattatc acgatgttaa ttgtagtctg taactatttg cgtatctttg
     2941 caatttaaaa taattattaa aatgcataaa gcaggttttg taaatattat aggaaatccg
     3001 aatgtcggta aatctacatt aatgaacgca ttagtaggtg agcgaatatc gattattacc
     3061 tctaaatcgc aaaccactcg ccatcgtatc atgggaattg taaatggaga tgactttcaa
     3121 attgtatatt cagatactcc gggagttttg aaaccgcatt atcgtttaca agaatcgatg
     3181 ttagaggctt ctcaatcggc tttgtctgat gctgatgtgc ttttgtatgt aacagatacc
     3241 ttcgagacat acgataaaaa ctccgagttt ttggagaaaa cgaaaagatc aggagtccct
     3301 acatttgtaa ttattaataa aatagatctt ttaccatcgc aagaggcatt agagaatttg
     3361 gtagaaaaat ggaaatcagt tttgccggac gctattataa ttccggtttg tgcattggaa
     3421 cgttttaacc tagaccaatt actgaaacaa atattggctt tgcttcccga gtcaccacca
     3481 tatttcgata aagatgcttt aaccgatagg ccggctcgtt tctttgttac tgaaattatt
     3541 cgcgagaaaa ttttagaaac ttatgataaa gaagtcccat attctacaga agttgtagtt
     3601 gagaagtttc aagaagatga aaagttgata cgtatcaatg caatgattat tgtagaaaga
     3661 gattctcaaa agggcatcat aatagggaaa ggcggttcta tgcttaaaaa agttggtatg
     3721 gcagccagaa ccgaaataga agcgtttctc gaaaagaaag ttttccttga gttgtatgta
     3781 aaagtagaga aggattggag aaataaggat aagcaattaa aatcttttgg atataacatt
     3841 gtccaaccat aattgctatt ccgttaatgt gttaatttta aactaagtat atgtcaatag
     3901 tagctattgt aggtcggcca aatgttggaa aatcaacttt gttcaatcgt ataacagaaa
     3961 caagaagagc gattgtaagt gatgaggcgg gtacaactcg tgatcgtcag tatggtaaag
     4021 cagaatgggg taaaaaagaa ttttcgttaa tagacaccgg tggacttgta gcaaattcgg
     4081 gggatatgtt cgaaaaagaa atcaagaaac aagtgaatat tgcgattgat gaggctgatg
     4141 taattttgtt tttagttgat gttcaaacag gtatatccga gttggatatg gatgtagctg
     4201 ctattttgcg tgcatgcaaa aaaccggtat tattagttgc aaataagaca gatgaaaatg
     4261 aatttcaata tcaggcagca gaattttatc gtttaggaat aggcgatcct tattgtgtat
     4321 cggctgcaac aggttttggt accggcgatt tgttggatgc tattgtacca ttgctttctg
     4381 aacgtgaaat tgaatatgtt gatgattctc ttccaagaat agctattgta ggtcgaccaa
     4441 atgccggaaa atcgtctttg cttaattctt tgatggatga ggaaagaaac attgtaacag
     4501 atgttgcagg tacgactcgc gattctatat atacaaagta taataaatat ggtaaggaat
     4561 tttaccttgt tgatactgca ggtatacgta aaaaagcaaa agtaaatgaa gatcttgaat
     4621 tctattctgt aatgcgtgcc attagaacaa tagagaactc ggatgtatgt ataatgatga
     4681 ttgatgccac aagaggtatt gagagtcagg accaaaatat tttttcttta atacaaaaga
     4741 ataagaaagg tttggtagta tgtgtaaaca aatgggattt ggtagaaaat aaagaacaaa
     4801 aagcaattac tacctttgaa aatgcaattc gcgagcgttt tgctccattt gtagattttc
     4861 caattgtatt tacatcagca acaacaaagc aaagaatatt taaggtaata gatgtggcac
     4921 ttgaagttta tggtaataag aaacgtcgta tcggaactaa taaacttaac gaatttatat
     4981 taccactaat tgagaattat ccgccacctg cattgaaagg aaaatacatt aaaattaaat
     5041 atgtaactca attgcctgat acatatgtac caacatttgt tttctttgca aacttaccgc
     5101 aatacattaa agacccgtat aaaagatttt tggaaaataa gatacgcgaa acttgggatt
     5161 ttacaggtat tcccttgcaa ctattcttta gagctaaatc taccggaaga aaataatttg
     5221 ataataggac aaaaaaaaat agtttgtgaa ttcacaaact attttttttt atgatattgt
     5281 taagctaatt attcagcaac aacttcaata gtaatttcag cacttacttc tttgtgaagt
     5341 tttacgttta ctttgaatga accaagttct ttgatagcgt ctttcaaaac gatggtacgt
     5401 ttgtcaacct tcaaaccttg tttatcaagt gcttcagcaa tttgcaatga agttacagaa
     5461 ccgaatactt tacctgttga acttgctttt gtacctaagc taatggtcaa accattaagt
     5521 tttgcagcta attcttcagc atcagctttg atttttgcta atttgtgtgc acgttgtttt
     5581 aaattttctg ccaatacttt ttgagcagat tcagtagcta aaacggcttt gccggtagga
     5641 attaaaaagt tacgtccata tccgcttttt acggttaaaa catcatcttt gtatcctaaa
     5701 ccctgaacat cttcttttaa aataatcttc atgttttgtt tcctccttta attatttcat
     5761 catatcagtt acataaggaa gcaaagccaa gtgacgagca cgtttaatag cttgagctac
     5821 catgcgttgg tattttaatg aagtaccggt aaggcgacga ggtaaaattt taccttgttc
     5881 gttcaagaat ttctttaaaa attcaggatc cttatagtcg atgtatttga taccgctacg
     5941 tttgaaacgg cagtatttct tcttctttgt atccacagat tgtggagtta aatatctgat
     6001 ttcagattga ttctgtgcca taatttattt cctccttgtg cttattcgtt gttacttgct
     6061 tcagcagctt tcttaacagg ctttgcagct ttgtcttcgg caggagcatt ggttttttta
     6121 cgtctcttct cagaatactc gatagcatat ttctccattt tcgttgttaa gaaacggata
     6181 acgcgttcat cacgacggaa ttgagtttcg agtgtgttta caatttccgg ctctgccgta
     6241 aattcaagta attgataaaa tcctgtggat tttttttcga tgggatatgc aagtttgcgc
     6301 aatccccaat tttcttcgtt caagatctca gcattgttgc ttttcaatac agtcttgaac
     6361 ttgtcgaccg cttccttcat ctgaacatca gataaaacgg gagtcaaaat gaaaacggtt
     6421 tcgtaatggt tcataatgtc cttttttaat tgttaataat taattcttta gaataaggct
     6481 gcaaaggtag tgattaattt ttgaattttg tatatcacat aataaaaaaa aactatattt
     6541 gcagacataa tcaactcact tactatggac actataattt tacttttgtt tttgtttact
     6601 ctattcctgt tttggaagaa gcctgataaa tggtatggtt tgcttatgtt cggactatca
     6661 gctgtggcat tagtcctatt atttatgtat catgctacgt ctacattatc aattaattta
     6721 taaggaattt ttatgcaaac ttcaaaatta ctttatttcg gctactgtgc caatatattt
     6781 gtattaacgg gagtcttatg cggaagccta tatatacaat tggtattaaa tgaatttcct
     6841 tgtcccttat gtatgttgca acgtatggca atgatattgt gtgcattatc gcttgtttac
     6901 atgctatcta aaactttgta ttacggatat ccaaatgcat ctgccgtatc tgttagcaat
     6961 ggacttaata ttgtatcatc tcttgtagga gcatctatat caattcgtca aattttattg
     7021 catattatgc cgagtgatcc gggttatggt gatgctattc tcggatttca tatatatacc
     7081 tgggctctaa tagtatttat atgtgagata gctttttctg caggtgtaat gttgcttaat
     7141 ccaaatcact gggataagtt gccattgtct tggcacaaac ctattaaagt gttattttac
     7201 tggttagcga ttgttgtagt aatttttttg ttggcaacat ttaatgaaga aggatttaat
     7261 tgggtcttgc ctgataaccc cacagaaaat gcgtttatca atttttgata tatagtattt
     7321 ttactctatg tcaattggta attgatgcga gtttttaacc tcgcctaaaa caaatgagct
     7381 attaagagaa ccgatgcagt ctaaatcccc aagtatacgt aatacgaaag attgatattg
     7441 tttcatattt tcagcatata ttttcagcaa aaaatcataa tctcctgaaa tgttgtaaca
     7501 ttcaacaatt tcggggatat tttgtactgc gctcattatg gcagttgcat tctcgtgaga
     7561 gtgctgtttc atcttcaaat aacagaatgc cataaatcct ttattcaatt tttctgcatc
     7621 aagaatcgct tgatagcctt taatatatcc ttcacgttct aatcttcgta atctttcaaa
     7681 cacaggagtt ggagataaat gtacttttaa agcaagttcc ttagtagtta attgcgaatc
     7741 tttttgtaat tgctgtaaaa tcagaaggtc tgttttatct aaatttccca tatcgtgttt
     7801 tattttataa attctaataa tatgcgctta ggatgaaatc taatctttat gcatccattt
     7861 aatacaaata ttctgtttta tatgcacatt tacttttgct gcagtaagat ttaactgtaa
     7921 atcaccgcaa atatagtgat gttttcttaa aaaatgcttt ttattggatt ttatttcgca
     7981 acaaaatacc tttgtcacat caaaacgaac aaatataaac atttaaacag ataaaaatat
     8041 gggaacaaaa aaattgcatt ttgaaacgct tcaaattcat gtgggtcaag aaaaagctga
     8101 tccatttact gattctaggg cagtacctat ttatcagacc acttcgtatg tatttcacaa
     8161 ttcggctcac gctgcagctc gctttaattt
//
LOCUS       DAOPID010000047         8141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_11161_length_8141_cov_4.075068, whole genome shotgun sequence.
ACCESSION   DAOPID010000047 DAOPID010000000
VERSION     DAOPID010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 8141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8141
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11161_length_8141_cov_4.075068"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="PK641_02765"
     CDS             complement(<1..915)
                     /locus_tag="PK641_02765"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPG55310.1"
                     /translation="MNHIHQIKGVFILGAIVLALSACNKGTSNTTGWKYNDAKYGGFQ
                     VVDNYEQDTPPGMVLVGGGTFTMGRVNEDTYAEWNNYPRRVTVNTFYMDQYEISNRDW
                     KEYVYWVNLMYGSSVSEKCRPDSTVWREELAYNEPYLNYYFTHVAYEMYPVVGVSWEQ
                     ATDYCVWRSDRVNERILKENGVVDFPDFAEIYRDSTKETSDTIAKQKVFNTAKYLKSN
                     EYNPDLKKSSVKTEFGDVRKTNMSDGLLLPEYRLPTEAEWEFAAYGITSEEGMENYEA
                     KRIYPWDGAQTRNPNKKARGKILANFARE"
     gene            1121..1879
                     /gene="folP"
                     /locus_tag="PK641_02770"
     CDS             1121..1879
                     /gene="folP"
                     /locus_tag="PK641_02770"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPG55311.1"
                     /translation="MSINIGGKLHCFDKTEIMAIVNFTPDSFFQGSRYNMEVPSGVFC
                     IDFGAYSTRPGAEEVSESEEIKRIDENLPILMSKYPDVAFSVDTFRPSVAKMCIEKYG
                     VHIINDVSGGCDEMFNVVAEYKVPYILMYPGGGGLPEMMYFFSKQIDALTRLGVNDII
                     LDPGFGFGKTLEENYDIARNLHALHTFELPILVGISRKSMIQKVLQTSPSESLNGTSV
                     LHTMLLDEGIDILRVHDVKEAMETIKLHTLYHKF"
     gene            1885..2658
                     /gene="cdaA"
                     /locus_tag="PK641_02775"
     CDS             1885..2658
                     /gene="cdaA"
                     /locus_tag="PK641_02775"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HPG55312.1"
                     /translation="MFSILGFRLKDLFDIIIVAILLYQVYRLMKGTSGMNIFISVLSF
                     IVIWFFVSYVFQMELLGAILNKVVNVGAILLIVLFQDEIRTFLNKLGGRHSKGLYAKI
                     RHMVIGGKGNRNDAFIDEIVQAAESFSKTKTGALIVIQKEADLTNYWETGERIDSEVR
                     ARLMENIFYKNTPLHDGALIIVGGRIKAVGCILPISHDQNIPQNLGLRHRAALGISEK
                     TDADVVVVSEENGKISYAEKGKLKIGVKTEELLKFLTKQ"
     gene            2809..4305
                     /locus_tag="PK641_02780"
     CDS             2809..4305
                     /locus_tag="PK641_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HPG55313.1"
                     /translation="MNYKILSAREAAEFVNDGDVVGFSGFTPAGSPKEVPTAIAERAK
                     AIHAAGKPFKIGMYTGASTGDSLDGALARANAIAFRTPYQSNKDLRNALNEIDGPNYI
                     DIHLSQLAQEIRYGFMPKPTVAIIEACDINDNGEIILTSGVGIAPTIARLADRIIIEL
                     NSRHTKELRGIHDIYEPLDPPARHEIPVFSPEKRIGSPILKVDPAKIVGIVETNRKDE
                     VGGFTEPDEITSRIGKNVSEFLSGEIKKGHIPESFLPIQSGVGNIANAVMAELGNNPN
                     IPAFKMYTEVIQDSVIDLMRNGRVTFASGCSLTVTDPVLQEIYDNMDFFRDKIVLRPE
                     EISNNPGLVRRLGLITINTALEADIYGNINSTHVMGSKMMNGIGGSGDFTRNAYISIF
                     TTPATAKDGKISSIVPMVTHVDQNEHSTKVLITEYGIADLRGKSPRQRAELIIENCVD
                     PIYRPMLHDYLKIAAKGQTPHNLGACFGMHRAFMATGDMRNTNWADFK"
     gene            complement(4368..7202)
                     /gene="polA"
                     /locus_tag="PK641_02785"
     CDS             complement(4368..7202)
                     /gene="polA"
                     /locus_tag="PK641_02785"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPG55314.1"
                     /translation="MKKLFLIDAYAIIYRSYYAFIKTPRINSKGLNTSAIYGFVNTLE
                     EVLKREHPTHIAVAFDPSGPTFRHEAYEKYKAQREATPEDIRKAVPIIKDLVSAYRIP
                     ILQVDGFEADDVIGTMAKKAEKAGFKTYMLTPDKDYAQLVSDNIFMYRPKHSGGYETM
                     GPEEVKIKYGLTSQEQVIDLLGLMGDSSDNIPGCPGVGEKTAAKLLQEFGSIDNLLSS
                     TDKLKGALKLKIEENKDQIIFSRFLATIRKDVPIELDEESLKTEEPDDAKLQAMFQDL
                     EFRSRLTTASPVNVINKVNSPKPATGPVQTSLFDLFDAPNQAPADTQKEEPTESSAEN
                     QIEVNQNLHLSDINNIKHKYTRIADADSLKNFVEELSHQPAFCFDTETTDIDVFEAEL
                     VGLSFCWKAGEAYFIPVDANNQKEVLDVLRPIFEDCSVSKIGHNIKFDMMILARSGVN
                     VCGDIFDTMIAHYILQPELRHGMDYLAEIYLQYRTIHYDDIVGPKGKNRIFIRDVDAN
                     LLCDYASEDADITYRLKTILEEELKKSKSESLFYDIEMPLVRTLTEMELAGVRIDSEA
                     LKQSSIQMTETMKALENEVQAFAGDGFNINSSKQVGELLFEKLKVVEKAKKTKTGQYI
                     TSEDVLETLRSKHPVVSKILEYRGLKKLLSTYIDALPLLVKKQTGKIHTSYNQAVTST
                     GRLSSSNPNLQNIPVRDEQGREIRKVFIPDDGCIFFSADYSQIELRLMAHLSGDENMI
                     EAFRAGDDIHASTAAKIFKLDISEVSSDMRRKAKTANFGIIYGISVFGLSERLTIPRA
                     EAKQLIDGYFETYPQVKLYMDESIEHARKLGYVETIFGRKRFLPDIKSANSIVRGYAE
                     RNAINAPIQGSAADLIKIAMVRISERFRKEGLRSQMIMQVHDELNFNVYPDELERVKY
                     IVVDSMEHAAELSVSLRADCGIGKNWLEAH"
     gene            7292..7912
                     /locus_tag="PK641_02790"
     CDS             7292..7912
                     /locus_tag="PK641_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HPG55315.1"
                     /translation="MLPITFTHILDFIGTFAFAISGIRMASSKNFDWFGAYVVGFVTA
                     IGGGTLRDILLNVSPFWMQNPSYVFITAFALLFVILFRKQIVKLEYTIFTFDTIGLAL
                     FTVVGIERTLASGFHFWIAIIMGIMTGAVGGIIRDILINEVPLIFRKDIYAMACVAGG
                     VVYFICYKFNCNIILTEVLSAVAVIATRVLAVKFHITLPVLHSEDE"
BASE COUNT         2538 a         1544 c         1418 g         2641 t
ORIGIN      
        1 ctctcttgcg aagttggcca agattttacc acgagccttt ttattaggat ttcttgtttg
       61 agcgccatcc caagggtaaa tacgttttgc ttcataattt tccatgcctt cttcagatgt
      121 gataccgtat gcagcaaatt cccattctgc ttcggtcggt aggcgatact caggaagtaa
      181 caaaccatca gacatattag ttttacgaac atcaccgaac tctgttttta cagaactctt
      241 tttgagatcc ggattatatt cattactttt aaggtattta gctgtattaa atactttctg
      301 ttttgctatg gtatctgaag tttcttttgt tgagtctctg tagatttcag caaaatcagg
      361 aaaatctaca acaccatttt cttttaagat acgttcatta acacgatcag aacgccaaac
      421 gcagtaatca gtagcttgtt cccatgaaac acctactaca ggatacattt cgtatgcaac
      481 gtgtgtaaaa taataattca agtatggctc attataagca agctcctcac gccaaactgt
      541 tgagtcagga cgacattttt cagaaacaga acttccgtac atcaaattaa cccaatacac
      601 atattctttc caatcacggt ttgaaatttc atattgatcc atgtagaatg tgttaacagt
      661 aacacgacgt ggatagttat tccattcagc gtatgtatct tcgttaacac gacccatagt
      721 aaatgtacca ccacctacaa gtaccattcc cggaggggta tcttgctcgt agttatctac
      781 aacttgaaat cctccgtatt tggcatcatt atatttccaa ccggtggtat ttgaagtacc
      841 cttattacaa gcagaaagtg ccaaaactat agcacctaga ataaagactc ctttgatttg
      901 atgaatgtga ttcattttca ttgtattttt atactcaagg ttaatttaca ttcgtagcta
      961 caaagataac taattttgag ctctaatatt gtgttttgct ttattttttt tagtttttcg
     1021 ataaaatgac tgcaaagatg cagataattt ctaattttgc aaagatttat gacttgtttt
     1081 ttcaaatcat tacacattta tgcacaaacg atctcaatat ttgtctataa acatcggtgg
     1141 aaaacttcat tgttttgaca aaaccgagat tatggcaata gtgaacttta cacccgattc
     1201 gttttttcag ggaagccgat acaatatgga agtcccgtca ggtgtatttt gcatagattt
     1261 tggtgcatac tctacaagac ccggagcaga agaagtttca gaatcggagg aaataaaacg
     1321 aatagatgaa aacttgccca ttttaatgag taaatatccg gatgtcgctt tttctgtaga
     1381 tacatttcgt ccgtctgttg caaaaatgtg catagaaaaa tacggagtgc atattattaa
     1441 tgatgtatcg ggaggatgtg atgaaatgtt taatgttgtt gcagaataca aagttccata
     1501 catactaatg tatccgggtg gtggtggact tcctgaaatg atgtatttct tttcaaaaca
     1561 gattgatgca ctaacgaggc ttggagtaaa tgacatcatt ttagatcccg gattcggttt
     1621 cgggaaaact ctggaagaaa attacgatat cgcccgtaac ttacacgcct tacatacttt
     1681 tgagttgcca atattagttg gtatatcaag aaaatcgatg atacaaaagg ttttgcagac
     1741 ttctccaagc gaatcattaa atggtacatc cgttttacat accatgctac ttgacgaggg
     1801 cattgacata ttacgtgtac atgatgtcaa agaagcaatg gaaactatca aattacacac
     1861 actctatcat aaattttaat aaacatgttc agcatacttg gattcagact taaagaccta
     1921 ttcgacatca ttattgtagc aatattgcta tatcaagtat atcgtttgat gaagggaacc
     1981 tccggtatga acattttcat atctgtgctt agtttcattg tcatttggtt ctttgtgtca
     2041 tacgtttttc agatggaatt gcttggtgct atcttgaaca aagtggtaaa tgttggtgcc
     2101 atacttctaa tcgttttatt tcaggacgag atacgcacat ttttaaataa gctaggcgga
     2161 agacattcaa aagggctata tgcaaaaata aggcacatgg ttataggagg aaagggtaac
     2221 agaaatgatg cttttataga tgaaattgta caagcagccg aatctttttc aaaaacaaaa
     2281 accggagctt taatagttat acaaaaagaa gcagatctaa caaattattg ggaaaccggc
     2341 gaacgtatag attcggaagt aagagctcgc ttaatggaga atatttttta taaaaacact
     2401 cctttacacg atggtgcttt aattattgtt ggaggaagga taaaggcagt tggctgcatc
     2461 ttgcctatat cacacgacca aaatattcct caaaatttag gtttaagaca tagagctgca
     2521 ttaggtatat cagaaaagac tgatgctgac gtagttgtag tgtcagaaga aaatggtaaa
     2581 atctcatacg ccgaaaaagg caaacttaaa attggtgtca aaaccgaaga attactcaaa
     2641 tttttgacaa agcaatagag attttgagac ttttagagga gaatgctagg ggctcagtca
     2701 aacctccccc tcccccttta tatcaaaact gtcaattttt ttttatttaa aaacaaattg
     2761 cactaccttt gtatgtcttt ttcaaaaaac gtcaaaaata tatattaaat gaattacaaa
     2821 attctttccg cccgggaagc ggcggagttc gtgaatgatg gtgatgttgt aggatttagt
     2881 ggttttacgc ctgcaggatc tccaaaagaa gtaccaacag ccatcgctga aagagcaaaa
     2941 gcaatacatg ctgccggtaa accttttaaa ataggcatgt acacaggagc atcaacagga
     3001 gattctcttg atggtgcatt agccagggcc aatgcaattg cgttccgtac tccttatcaa
     3061 tcaaacaaag acctgcgtaa tgcgcttaac gaaattgatg gtcctaatta cattgatata
     3121 catttatcac agttggcaca agaaattcgc tacggattta tgccaaaacc aacagttgca
     3181 attattgaag catgtgatat taacgacaat ggcgaaataa ttcttacctc aggtgttggt
     3241 atcgctccaa caattgcacg tctggcagac cgcataataa ttgaattaaa tagccgtcac
     3301 actaaagagc tacgcggtat acacgatata tatgaacctc ttgatcctcc tgcacgtcac
     3361 gagatacctg tttttagtcc tgaaaaacgc ataggaagtc ctatcttaaa ggttgatcct
     3421 gcaaaaatcg taggtattgt agaaacaaac agaaaagatg aagttggtgg attcacagaa
     3481 cctgacgaaa ttacctcacg cattggtaaa aacgtatcag aattcttatc cggagaaatc
     3541 aaaaaaggac acattcctga atcattcttg ccaatacaat caggtgtagg taatatcgca
     3601 aatgcagtta tggccgaatt aggtaacaat cctaatatcc ctgcattcaa aatgtacact
     3661 gaagttatac aagattcagt tattgatttg atgcgtaatg gacgtgttac atttgcaagc
     3721 ggctgttcac ttacagttac cgatcctgtg ctacaagaga tttacgacaa tatggatttc
     3781 ttccgcgata aaattgtttt acgtcctgaa gaaatttcaa ataacccggg tcttgttcgt
     3841 cgtttaggct taattacaat taatacagct cttgaagctg atatttatgg aaacataaat
     3901 agtacacacg taatgggttc taagatgatg aatggtattg gtggctcagg agactttaca
     3961 cgtaatgcat atatctcaat ttttacaact cctgctactg caaaagacgg aaagataagt
     4021 tctattgtac ctatggtaac acacgtagat caaaatgaac actcaacaaa ggttctcatt
     4081 acagaatatg gtatcgcaga tttaagagga aaatcaccac gtcaacgtgc tgaattaatc
     4141 atcgaaaatt gtgtagatcc aatatacaga ccaatgttac acgattattt aaaaattgct
     4201 gccaaaggtc aaactccaca taacttaggt gcttgctttg gtatgcaccg cgcattcatg
     4261 gctacaggag atatgcgtaa tacaaattgg gctgatttta aataagaaga tcttataatg
     4321 atgcaaaaaa aagaatcaca taaatttatg tgattctttt tttataccta gtgtgcttcg
     4381 agccaatttt tgccgattcc acaatcagca cgtaaagaga ctgatagctc tgcagcatgc
     4441 tccatcgaat caaccactat atattttacc ctttccaact catcaggata tacattaaag
     4501 ttcaactcat catgcacttg cataatcatt tgagaacgaa gaccctcttt cctaaaacgt
     4561 tctgaaatac gcaccatggc aatcttaata agatctgcag cacttccctg tatcggagca
     4621 ttaatagcat tccgttctgc atatcctctt acaatactat tagctgattt tatgtcaggc
     4681 aagaaacgtt tacgtccgaa aattgtttca acataaccta attttcttgc atgctctata
     4741 ctttcatcca tgtataactt tacctgagga taggtctcaa aatacccatc aataagttgt
     4801 ttagcctctg ctcttggaat agtcaaacgt tctgataaac caaaaaccga aataccataa
     4861 ataattccaa agtttgctgt tttggcttta cgcctcatgt cagaactaac ttcactgata
     4921 tcaagtttaa atatttttgc tgcggttgat gcatgtatat catctcctgc acgaaaagcc
     4981 tcaatcatat tctcatctcc actcaaatgt gccatcaaac gtaattcaat ttgagaataa
     5041 tcggccgaaa agaatatgca tccatcatcc ggtataaaaa cctttcttat ttctctgcct
     5101 tgttcatctc taacaggaat gttttgcaaa ttagggttac ttgaacttaa tcttcctgta
     5161 gaagtaacag cctgattata agaagtatga atctttcctg tctgcttttt tacaagcaat
     5221 ggtaaggcat caatataagt actcaataac tttttcaaac ctctgtattc caatatctta
     5281 gatacaacag gatgcttaga ccgcaatgtc tccaatacat cttctgaagt tatatattga
     5341 ccggttttag ttttttttgc cttttctaca accttcaatt tttcaaaaag caactcaccc
     5401 acttgctttg aagaattaat attaaaacca tctcctgcaa atgcttgcac ctcgttttct
     5461 aaagccttca ttgtttcagt catttgtatt gacgactgtt taagtgcttc agaatcaatt
     5521 cttacacctg ccaattccat ttcggtaaga gtacgaacca agggcatttc aatatcataa
     5581 aacaaagatt cggatttgct cttttttaat tcttcttcta aaattgtttt taggcgatac
     5641 gtgatgtcag catcttctga tgcataatcg cacaaaagat ttgcatcaac atctcttata
     5701 aaaattcgat ttttaccttt tggccctact atatcatcgt aatgaatggt acgatattgt
     5761 aaatatattt ctgccaaata atccattccg tgacgcaatt ccggttgtaa gatatagtgt
     5821 gcaatcattg tgtcaaaaat atctccacac acatttacac ctgatcttgc caaaatcatc
     5881 atatcaaact taatattatg accaatcttg cttaccgaac aatcttcaaa tataggtctt
     5941 aatacatcta agacctcctt ttgattattt gcatccaccg ggataaaata agcttctcct
     6001 gctttccaac aaaacgacaa gcccacaagt tcagcctcaa aaacatcaat atcagttgtt
     6061 tccgtatcaa aacaaaatgc cggttgatgg cttaattctt ctacaaaatt ttttaaacta
     6121 tcagcatcag caatacgagt atatttatgc ttgatgttgt taatatcaga taaatgcaag
     6181 ttttgattta cttctatctg attctctgcg cttgattcag taggctcttc tttttgagta
     6241 tcagcaggag cttgatttgg tgcatcaaac aaatcgaata atgaagtctg tacaggacct
     6301 gttgccggtt ttggactgtt tactttatta attacattaa cgggcgatgc agttgttaac
     6361 ctgcttctaa actctaaatc ttggaacatt gcctgaagtt tagcatcatc aggttcttct
     6421 gtttttagtg attcctcgtc aagttcaata ggcacatctt ttctaatagt cgccaagaaa
     6481 cgcgaaaata taatctgatc tttattctct tcaatcttta atttcaatgc tccttttagt
     6541 ttatcggttg aagacaacaa attatcaata ctgccaaact cttgcaacaa cttagcagca
     6601 gttttctctc ctacgccagg acaacccgga atattatcac tgctatcacc cattaatccc
     6661 aacaaatcta ttacttgttc ttgcgatgtc aaaccatatt tgattttcac ctcttcaggt
     6721 cccattgttt cgtatccgcc actgtgttta ggacgataca taaaaatatt gtctgatact
     6781 aattgggcat aatccttatc aggagttagc atatacgttt taaatccggc cttctcagct
     6841 ttctttgcca ttgtgccaat cacatcatca gcctcaaaac catcaacttg taaaattggt
     6901 atgcgatatg cagaaactaa gtctttaatt atgggcactg cttttcgaat atcctccgga
     6961 gttgcttcac gttgagcttt atatttttca taggcttcat ggcgaaatgt aggacccgaa
     7021 ggatcaaaag caactgctat atgcgtagga tgctcgcgct ttaagacctc ttccaaggtg
     7081 ttcacaaatc cataaatggc tgatgtatta aggccttttg aatttattct gggagtcttt
     7141 atgaaggcat aatatgaacg atatataatt gcataagcat caatcaaaaa aagtttcttc
     7201 atatttaaat gttttttgtt tcgtaaaggt aagaaaaaag ctaaaatttt acgttacttt
     7261 gcagctaaat tatacgtcat tataaagcaa catgttacca ataacattta cacacatttt
     7321 agactttata ggcacttttg cttttgcaat aagcggtatt cgtatggcct catcaaaaaa
     7381 ctttgattgg tttggagctt atgtagtcgg ttttgtaact gcaatcggag gtggtactct
     7441 acgcgatatc ttacttaacg tatctccttt ttggatgcaa aatccatcgt atgtatttat
     7501 cactgcattt gcactattat ttgtgatatt atttagaaag cagatagtaa aacttgaata
     7561 caccatattt acttttgata ctattggtct agcccttttt acagtagtag gtattgaacg
     7621 tactctcgcc tcaggcttcc atttttggat tgcaattatt atgggtataa tgacaggtgc
     7681 tgtaggaggc atcattcgtg atattttaat taatgaagtt cctcttattt ttagaaaaga
     7741 tatttatgcc atggcatgtg tcgccggtgg agtcgtatac tttatatgct acaaatttaa
     7801 ctgcaacatt atactgacag aagttttatc ggcagttgct gtaatagcaa cacgtgttct
     7861 agctgttaaa tttcacatca cactacctgt actccacagc gaggacgaat aattaatggt
     7921 gaggggtgag gggttaaggg gcgtgtagtg tgtaatcgtg taatgtgtaa tgtgtaatgt
     7981 gtaatgtgta atgtgtaggg acgaattgca ttcgcccgtg ggatttgcat tgtgattatg
     8041 tttcgttgtg ttattttgta ggggcattat taatcatttc aacattggat ttatattaat
     8101 tgcattgtga atgtgaaggg cacgccatgg catgccccta c
//
LOCUS       DAOPID010000048         8072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_11304_length_8072_cov_4.129849, whole genome shotgun sequence.
ACCESSION   DAOPID010000048 DAOPID010000000
VERSION     DAOPID010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 8072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8072
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11304_length_8072_cov_4.129849"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1388)
                     /locus_tag="PK641_02795"
     CDS             complement(<1..1388)
                     /locus_tag="PK641_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPG55316.1"
                     /translation="MQTEISPYQLAHDFASFTSRSIFITGKAGTGKTTFLRNLHNESS
                     KSMAIVAPTGVSAIQAGGVTIHSFFQLPFHPFLPTEDGRRDLISKVHFVGSRRQIFKS
                     LELLVIDEVSMVRADVLDAIDTILRHYKYCPNLPFGGVQVLFIGDLYQLSPVYKEEEK
                     EILSPYYDGMYFFDSKVVKEFPPIYIELDKIFRQSDMNFIELLNEVRNNRLSKKNFEL
                     LENLYKPDFKPSSKEKYITLTTHNYKADTINLSELKSLKGGISKFKAIIKGEFPEKSY
                     PTEEVLELKLGARVMFLKNDRDRRFYNGKIGDVVGFDTDANTIMVKCSDSDDTIELQP
                     DIWENMSYTMNKDTRDIEEKLLGTFTQFPLRLAWAITIHKSQGLTFDKAMIDADRAFA
                     PGQVYVALSRCRSLDGIILLSPIHRRCLCLDSRVTDFGSNKQDFCTVSDQLLQEKSNF
                     ILNLVSDLCGFRV"
     gene            complement(1562..1637)
                     /locus_tag="PK641_02800"
     tRNA            complement(1562..1637)
                     /locus_tag="PK641_02800"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1601..1603),aa:Glu,seq:ctc)
     gene            complement(1696..3168)
                     /gene="cysS"
                     /locus_tag="PK641_02805"
     CDS             complement(1696..3168)
                     /gene="cysS"
                     /locus_tag="PK641_02805"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPG55317.1"
                     /translation="MEHKFFIYNTLTRKKEKFVPIHSPYVGMYVCGPTVYGDGHLGHA
                     RPAITFDLLFRYLQHLGYKVRYVRNITDVGHLEHDADEGEDKIAKKARLESKEPMEVV
                     QYYLNRYHKAMEALNVLPPSIEPHASGHIIEQIEFIKKIIESGYAYESEGSVYFDVEK
                     YSKRYNYGELSGRNIDELLETTRVLDGQSEKHRSTDFALWKKAQPEHIMRWPSPWSDG
                     FPGWHLECSTMGTKYLGEEFDIHGGGMDLIFPHHECEIAQSTAALGHQTVRYWMHNNM
                     ITINGQKMGKSLGNFITLDEFFTGKHDLLRQAYSPMTIRFFILQAQYRSTVDFSNEAL
                     QASEKGMQRLMEAYRRLNSLTVSAQSTIDVKGLRKSCEDAMSDDLNSPIVISHLFDSV
                     KSINAVKDGKATISSEDLEELKSVFKLFIEDILGLYDESATGNSSNTEAYKKAVDLLL
                     DIRMQAKKNKDWATSDKIRNELTASGFTINDTKDGFEWSI"
     gene            3282..6104
                     /locus_tag="PK641_02810"
     CDS             3282..6104
                     /locus_tag="PK641_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPG55318.1"
                     /translation="MKKIFIILSNILFLTTLISAQTVTTNPSIVQDGYTGDVTIIYNP
                     AGGPMASATNAYAHIGLITSASSDDSDWQCAPANWLDKSVKYKMTKSGSNWTLNIGNM
                     YTYFSACASDTFEKIAVVFNDGVVGGKEGKDPNGKDFFVDIAPAGLAVLFDGTSSSGI
                     VNSGASVTLIANATASVNLVIKKNGTSVKTASGTSITHTETLSSAGDYVYEVYGNGTL
                     YDSRTVSVISAPVNAARPSGLLEGITYNSSDNTKVHLCLYAKDNANVLPDNIFVIGDF
                     NNWTPSNSYQLKKDGSTGYWWTEITGLTSGREYAFQYLVKIGSKTVKISDPYSEKVLH
                     PDDQWEPKTNYPNLMDYPSKGEGYVSVLQTNKPEFNWSSATTNFYKPNKNNLVIYELW
                     VHDFSPQHSFRSIINRLDYISNLGVNVLELMPVTEFDGNKSWGYNPTHYFALDKAYGC
                     ENDLKTLIDECHKRGIAVVLDMVFNHVTGSAPQARLYWGTNSIATNNPWFNQSAPHGG
                     SVNQDWNHNFEPTRNLMKRALKYWIDEYKVDGYRMDLSHGFCGPSCNERTAIMFDYYN
                     NGVKAGNQYAYFILEHWEESSGERGNYINNGMMCWSYNNSQTGTAFSQTAMGWLRDGD
                     ALDGAGMDGWISYSESHDEQRNFWKSKMWGNSDFKTNVASRMNRVPLNVAFGALQDGP
                     HMLWQFQELGYDYSICRSESGLYDSNDTGSANCSRVDPKPIPEALSWYTNADRMNSYK
                     KIGQILQLRTKIKPTVFEGNPTSTDLGSGKAARSIIWGSGNDRIFIVGNFNVPSGSEF
                     VGSVNITLPSGNSWFDYLANGNNSLSAGTSISLSPGEVKIYTASRLTLPSVPNVYNDF
                     VGIEETTTDPDLICNVYPSLVDDYVTIDSPEGIRRIDVVGLNGETIKLGLSDSKVYNL
                     AGLPKGPYLVVVSFDKKQQVYKIIKK"
     gene            complement(6173..7117)
                     /locus_tag="PK641_02815"
     CDS             complement(6173..7117)
                     /locus_tag="PK641_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HPG55319.1"
                     /translation="MYINAMGMFVPQGRIINKYFEDTLGLTDEWLLQRTGIHSRSKTA
                     DNEDGYSMGIDAVTDAKTNLSYNIEDVDLIVSASYTILDTVGTLAHRVQRHFNITNAK
                     ALAVTSACSSFVNGVEIIEGYFAMNKASKALIICSEENSAYNDLTNPQSGHLWGDAAV
                     AVFISKERQSESDAEILDVTTLGLGCIGKGPGGVGLFPKTTGITMHDGRDVFQHACIY
                     MPKTLEILLHKNNLTINDVSYFACHQANLRIVSHIANQWNLPDDKFLNNISRLGNTGS
                     PSSMLSVVQNKDKFKKGDLIGLSVFGGGYSSGAMLIRF"
     gene            7422..>8072
                     /locus_tag="PK641_02820"
     CDS             7422..>8072
                     /locus_tag="PK641_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPG55320.1"
                     /translation="MRVYQTNEIKNISLVGSSGSGKTTLAEAMLFESGLVKRRGSVEA
                     GNTVSDYFPVEHEYGYSVFPTVLHVEWLNKKLNFIDCPGSDDFVGGLITSLSVTDTAL
                     MLINAQHGVEVGTQNQYRQIDKMGKPCIFVINQLDHEKADYENSIENLKENYGAKVIP
                     IQFPISVGPSFHALIDVLLMKMYQWKPEGGVPEILAIPEEYMQKAKAFIYCAEEDFG"
BASE COUNT         2639 a         1461 c         1424 g         2548 t
ORIGIN      
        1 actctaaatc cgcataaatc gcttactaaa tttaggataa aatttgactt ctcttggagt
       61 aactggtctg atactgtaca aaaatcttgc ttattacttc caaagtctgt aacacgacta
      121 tcaagacaca aacaacgcct gtgtatagga cttaaaagaa taatgccgtc taaacttctg
      181 catctgctta gcgctacata tacctgaccc ggtgcaaatg ctctatcggc atcaatcatt
      241 gccttatcaa aagtaagacc ttgactttta tgaattgtaa tcgcccatgc taatcgtagg
      301 ggaaattgag taaatgttcc taatagtttt tcttctatat cacgcgtatc tttgttcatg
      361 gtatatgaca tattttccca tatatccggc tgtaattcaa tagtgtcatc tgagtcagaa
      421 catttaacca taattgtatt agcatctgta tcaaaaccta ctacatcacc tatcttgcca
      481 ttataaaaac gtctatctct atcgttcttt aaaaacatta cacgagctcc tagcttgagt
      541 tccaatactt cttctgtagg atatgatttt tcagggaact cccctttaat gatggcttta
      601 aatttactta tgccaccttt taatgatttt aattcgctta aattaattgt gtctgcttta
      661 taattatgcg ttgttaatgt aatgtatttt tctttagaag atggcttaaa atcaggttta
      721 taaagatttt ctaagagttc aaagttcttt ttgcttaaac ggttatttct aacctcattt
      781 aatagttcga taaaattcat atcactctgc ctgaaaattt tatctagctc tatatagata
      841 ggaggaaatt ctttgacaac tttactatca aagaaataca tgccatcata atatggagag
      901 agtatttctt tttcttcctc tttataaacc ggcgataact gatataaatc gccgataaat
      961 agcacttgaa caccaccaaa agggaggttt ggacaatatt tataatgtct taatattgta
     1021 tcaatagcat ctaaaacatc tgcacgcacc atactcacct catctattac aagcaattct
     1081 agagatttga aaatttgacg gcgcgaacca acaaaatgta ctttagaaat taaatcacgt
     1141 ctgccatctt ctgtaggcaa aaaaggatga aacggtaact gaaaaaatga atgaattgtt
     1201 actccacccg cctgtattgc agatacaccg gttggtgcaa caattgccat tgatttacta
     1261 ctttcattat ggagattcct taaaaaggtg gtcttcccag ttccggcttt accggtgata
     1321 aatatagaac ggcttgtgaa agacgcaaaa tcgtgagcta attgatacgg agaaatttct
     1381 gtttgcatga tatcgaattt tgatgggata aattttgatt catgcaaaga tattgaaaat
     1441 tgagttaatt caagataatt tgtcggtttt tacaactttg aaaagatttt ttatgatgta
     1501 tttgtattta attaaaatga agtaaaaaaa atgcaggaat gaaaaatcat tcttgcattt
     1561 ttggtagcgc aaccgggaat cgaacccggg tttccgccgt gagagggcgg cgtcctaggc
     1621 cactagacga atgcgccatt aaacgtgctg caaaggtaat aaaaaaatta ttacaaccgc
     1681 aaacttatat tacaattata tagaccactc aaaaccatcc tttgtatcat tgatggtaaa
     1741 tcctgaagct gttagctcat tacggatttt gtctgaagta gcccaatctt tattcttttt
     1801 tgcctgcata cgtatatcaa gtaacagatc aacagctttc ttgtaagcct cagtgttact
     1861 actattacct gttgcagatt cgtcatataa acctaaaata tcctcaataa agagtttaaa
     1921 tacagattta agttcttcaa gatcttccga tgaaatcgta gcttttccgt cttttactgc
     1981 attaatactc ttaacagaat caaacaaatg cgatattacg ataggtgaat taaggtcgtc
     2041 agacatggca tcttcgcaac ttttacgaag tccttttaca tcaatagtag attgcgctga
     2101 aacggtcaaa ctatttaaac gtctgtatgc ttccatcaaa cgctgcatac ccttttctga
     2161 agcttgtaat gcttcattac taaaatctac ggtactacga tattgagctt gcaatatgaa
     2221 gaaacggatt gtcattggag aataggcttg tctcaacaga tcgtgcttac ctgtgaagaa
     2281 ctcgtccaaa gtaataaagt ttcctaatga tttgcccatc ttttgtccat taatggtaat
     2341 catattattg tgcatccaat agcggacggt ttggtgtccc aaagcagcag ttgattgcgc
     2401 aatttcgcat tcgtgatgcg ggaaaattaa atccataccg ccaccatgta tatcaaactc
     2461 ctcgcctaaa tattttgtac ccattgtaga gcactcaaga tgccaaccgg ggaaaccatc
     2521 actccatggt gaaggccaac gcataatatg ttccggttgc gccttcttcc acaaagcaaa
     2581 atcggtacta cgatgtttct cgctttgtcc atcaagtaca cgtgttgttt ctaataattc
     2641 gtctatatta cgtccggaca actcaccata attgtatctt ttactgtatt tttcaacatc
     2701 aaagtatact gaaccttcgc tttcgtaagc ataaccggat tctattattt tcttaataaa
     2761 ttcaatttgc tctattatat gacctgatgc atgcggctca atacttggcg gcaatacatt
     2821 aagagcctcc atggctttgt gataacgatt tagataatat tgcacaactt ccattggttc
     2881 tttgctttcc aaacgagctt tctttgcaat tttatcttcg ccttcgtctg catcatgttc
     2941 aagatgacct acgtcagtaa tgttacgtac atatctgact ttgtatccaa ggtgctgcaa
     3001 ataacggaat aacaaatcaa atgtaattgc agggcgggca tgtcctaaat gaccatctcc
     3061 ataaactgta ggtccgcaca catacatacc aacataggga ctatgtattg gtacaaactt
     3121 ttcctttttt ctggttaacg tattgtatat aaaaaactta tgttccataa catatttgta
     3181 ttcgtttgtg cttacaaaga tagataaaaa caataaaata tttgaattat catttatcaa
     3241 atgttagttt tgcctcacat aaaccctata cactcattat tatgaaaaaa atctttatta
     3301 tacttagcaa tattttattt ttaacgactc tcatttccgc acaaacggta actacgaatc
     3361 catctatcgt acaagatggt tatacaggag atgtcaccat catctataat ccggcaggag
     3421 gaccaatggc aagcgctacg aatgcttacg ctcacattgg tttaataacc tctgccagct
     3481 cagacgattc tgattggcaa tgcgctccgg ctaactggtt agataaatct gtaaaataca
     3541 agatgacgaa aagcggtagt aactggacat taaacatagg caatatgtac acatacttta
     3601 gtgcatgcgc ctcagacacg tttgagaaaa ttgcagttgt atttaatgat ggagttgtgg
     3661 gaggcaaaga aggcaaagat cctaacggga aagatttttt tgttgatatt gctcctgcag
     3721 gtttagctgt attatttgat ggtacatcat catcgggaat tgtcaattcc ggagcatctg
     3781 ttacattaat agcaaacgct actgcatcag tcaatctggt aataaagaaa aatggcacat
     3841 cggttaaaac agcaagcggt acatctatta cacatactga aacactttca tcagcaggag
     3901 attacgttta cgaagtatat ggaaacggaa ctttatatga tagcagaaca gttagcgtca
     3961 tatccgctcc ggtaaatgcc gcacgtccaa gcggtctgtt agaaggtatt acatataaca
     4021 gttcagataa tacaaaagta catttgtgtc tttatgctaa agacaatgct aatgtcttac
     4081 ctgacaatat atttgtaatt ggcgatttta acaattggac tccttctaac tcataccaac
     4141 ttaaaaaaga tggatctacc ggatattggt ggactgaaat cacaggtttg acatcaggca
     4201 gagagtacgc attccagtat ttagtaaaga taggttcaaa aacagtcaaa ataagcgacc
     4261 cttattcaga aaaagtactt catcctgacg atcaatggga accaaagact aattacccta
     4321 atttaatgga ctatcctagt aaaggagaag gatatgtatc tgtactacaa acaaataagc
     4381 ctgaatttaa ttggagttca gcaactacaa atttttataa acccaataaa aacaatcttg
     4441 tgatttatga attgtgggtt catgattttt caccgcaaca ctctttccgc tctattataa
     4501 atagattgga ttacatctcc aatcttggag ttaatgtttt ggaattaatg cctgttacag
     4561 aattcgatgg taataagagt tggggataca atcctactca ttactttgca cttgacaaag
     4621 catacggatg cgaaaatgat ttaaaaacac ttattgatga atgccataaa agaggcatcg
     4681 cagtggtatt ggatatggtg tttaatcatg taacaggatc ggctccacag gcaagattgt
     4741 attggggtac aaactcaatt gcaacgaata atccttggtt taatcaatct gcacctcatg
     4801 gtggaagtgt taatcaagat tggaaccaca atttcgaacc aactagaaat ttaatgaaaa
     4861 gagcgcttaa atattggata gacgaatata aagttgacgg atatcgcatg gatttatcgc
     4921 acggcttctg tgggccttca tgcaatgaac gtactgcaat tatgtttgac tattacaata
     4981 atggtgtaaa agcaggtaat caatatgcat attttattct ggaacattgg gaagaatcat
     5041 caggcgaaag aggtaattac ataaataacg gcatgatgtg ttggtcatac aataactcgc
     5101 aaacaggtac cgcattctca caaacagcaa tgggttggtt aagagatggt gatgctcttg
     5161 atggcgctgg tatggatggt tggatttcgt atagtgaaag ccacgatgaa caacgcaatt
     5221 tctggaaatc aaaaatgtgg ggaaacagtg atttcaaaac taatgttgca tcacgtatga
     5281 acagagtacc tctaaatgtc gcatttggag cattacaaga tggtcctcac atgttatggc
     5341 aattccaaga attaggatat gattattcta tatgcagaag tgaaagcggt ttatatgaca
     5401 gcaacgatac gggtagtgct aattgcagca gagttgatcc aaagcccatt ccggaagcat
     5461 taagctggta tacaaatgca gatcgcatga attcatataa aaagataggt cagatattac
     5521 aactaagaac taaaattaaa ccgacagtat ttgaaggaaa tccaacaagc actgatttag
     5581 gatcaggcaa ggcagctaga agtataatat ggggaagtgg caatgatcgt atatttattg
     5641 tcggaaactt taatgttcca tcaggaagtg agttcgtagg gtctgtaaac ataactttgc
     5701 catcaggtaa ttcgtggttt gactatttag caaatggtaa taactcatta agtgcaggga
     5761 ccagcatcag cctaagtccg ggagaagtta aaatatatac agcatctagg ttgactttac
     5821 ctagtgtacc taatgtttac aatgactttg tcggcattga agaaactaca acagatcctg
     5881 atttgatttg taatgtttat ccttcattgg ttgatgacta tgttacaata gatagccctg
     5941 aaggaattcg tagaattgat gtagtaggtt taaatggcga gacaattaaa cttggcttat
     6001 cagatagtaa ggtatataat cttgcaggct taccaaaagg tccataccta gttgttgtaa
     6061 gctttgacaa aaaacaacaa gtatacaaga ttataaaaaa ataaaggcaa tctatattca
     6121 tacaaaaagg cagcaaaaaa attgctgcct tttgtatttc tgaactatta tattagaatc
     6181 taatcagcat tgctcctgac gagtaaccac caccgaatac tgataagcct atcaaatcgc
     6241 ccttcttaaa cttatcttta ttctgaacta cagacaacat agaacttggg gaacctgtat
     6301 tacctaaccg gctaatgtta tttagaaact tatcgtcagg aagattccac tgatttgcaa
     6361 tatgactaac gattcgcaaa tttgcttgat gacatgcaaa atatgataca tcgtttattg
     6421 ttaagttgtt tttgtgtaat aaaatttcaa gtgttttagg catataaata catgcatgtt
     6481 gaaacacgtc tcttccgtca tgcatggtaa tgccggtagt ttttggaaat aatcctactc
     6541 ctccggggcc tttccctata caacctaaac ctagtgttgt aacatcaagt atttcagcat
     6601 cagactctga ttgtctttct tttgatataa atacagccac agctgcatca ccccacaaat
     6661 gtccgctttg tggatttgtc aaatcattgt atgcggaatt ttcttctgag catattatta
     6721 aagccttgct tgccttgttc attgcaaaat agccttcaat aatttctact ccatttacaa
     6781 atgatgaaca tgcagaagta actgcaagtg ctttggcatt agtaatgtta aaatgacgct
     6841 gaactctgtg agctaaagtc cctaccgtat ccaaaatagt atatgacgca gacactatta
     6901 aatctacatc ctcaatatta taagacaaat tagtttttgc atctgttact gcatcaattc
     6961 ccattgagta gccatcttca ttatcagcag ttttggatct ggaatgaatt cctgtacgtt
     7021 gcaacaacca ctcatctgtc aatcctaagg tatcttcgaa atatttgttt atgattcttc
     7081 cttggggaac aaacattccc attgcattta tatacatata ttacaaactt attatgataa
     7141 ttattcgtta tttgagaact taaatatcag tgagtatatt attatttgtt gtcctaattt
     7201 ttagaaccgg gtgcaaatgt gtagaaaaac attgaattat taacaatctg caattaaata
     7261 ttggccaaaa attaattttc ttttgccaat actgttaatt tgtatcaaat tatatataat
     7321 ttatgctgtt aaacataaaa aagactattg atactacaaa ttaaatttcg taatttgcca
     7381 agccttagaa tttaaattta tcataaatta atccaattat tatgagagtc taccaaacta
     7441 acgaaatcaa gaacatttca cttgtgggaa gttcggggtc aggtaagacc actcttgctg
     7501 aagcaatgct ttttgagagt ggtctagtaa aaagaagagg aagtgtagaa gcaggcaata
     7561 ctgtttctga ttattttcct gttgaacatg aatatggtta ttcggttttc cctacagttc
     7621 tacacgtaga atggctaaac aaaaaactta attttataga ttgtcccgga tcggacgatt
     7681 ttgtaggtgg cttaataaca tcactgagcg ttacagatac ggctttaatg ctaattaatg
     7741 cacaacacgg cgtagaagta ggaacacaaa atcagtatag acaaatcgat aaaatgggca
     7801 aaccgtgtat tttcgtaatt aatcaactcg atcacgaaaa agccgattac gaaaattcaa
     7861 ttgaaaattt gaaagaaaac tatggagcta aagttattcc tattcaattt ccaatatctg
     7921 tcgggccatc ttttcatgca ttaattgacg tattattgat gaaaatgtat caatggaaac
     7981 ccgaaggtgg tgtccctgaa atactagcta ttccggaaga atatatgcaa aaagcaaaag
     8041 cttttattta ttgtgctgaa gaagattttg ga
//
LOCUS       DAOPID010000049         7960 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_11536_length_7960_cov_4.817837, whole genome shotgun sequence.
ACCESSION   DAOPID010000049 DAOPID010000000
VERSION     DAOPID010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 7960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7960
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11536_length_7960_cov_4.817837"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PK641_02825"
     CDS             complement(<1..476)
                     /locus_tag="PK641_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     cytochrome b subunit"
                     /protein_id="HPG55321.1"
                     /translation="MWLFDSSVGRKFVMSITGASLVLFLLFHATMNVVVVFSPEGYNA
                     ICAFLGANWYALIGTLGLAFLLLVHFVYAFILSIQNYRARGTQRYAVVERQEGVDWAS
                     KNMLVLGFIVIGFLLLHLYNFWFKMQFVEITGIETGPFSPTDGAAYVKELFSNPVY"
     gene            614..1192
                     /locus_tag="PK641_02830"
     CDS             614..1192
                     /locus_tag="PK641_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPG55322.1"
                     /translation="MMNQLKGTQTEKNLAIAFAGESQARNRYTFFASVAKKEGFEQIS
                     ANFLETAEQEKEHAKKFFKYLEGGMVEITASYPAGVIGKTIDNLMAAANGEREEWSKD
                     YPHFADIAEQEGFPKIAETFRKIATVEQMHEERYKNFIKNIQENKVFEKDEEVIWQCR
                     NCGYLHKGKKAPELCPACEHPQAYFEIKKENY"
     gene            1375..2565
                     /locus_tag="PK641_02835"
     CDS             1375..2565
                     /locus_tag="PK641_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPG55323.1"
                     /translation="MEYRRSQFKEIIKRINETRSKMQVIVGPRQIGKSTLIAQVLEEC
                     PIPFDSYSADDIVGVSADWLSQIWETQRLKISANAESKRLLVIDEVQKINNWSETVKA
                     EWDRDNREKLNLIVILLGSSRMLNEKGLTESLAGRFELIRLSHWSYSEMKDCFGWSLQ
                     QYIYFGGYPGAAQYINDENRWRNYVKDSLIETSISKDVLMESKIMKPQLLRQLFEIGS
                     SYSGELLSLTKIAAQLQDAGNVTTLAGYLHLLDECGLLCGLQKYADDFARRYNSVPKF
                     QVHNSALRNVYENDAFSEAIINPKTWGRCVESAIGAYLVSQSKIKDYKLYYWRDSSDE
                     VDYILVQRNKIIAIEVKSGRRTSNKGLSKFINLYHPHKAFVVGSGGFSLEEFFTLDLS
                     ILFK"
     gene            2736..3395
                     /locus_tag="PK641_02840"
     CDS             2736..3395
                     /locus_tag="PK641_02840"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPG55324.1"
                     /translation="MERFKQFLHNNALFLYPFIILWIIMLCFALMHSKTETHYLLNTW
                     HGSFGDVFFQYVTYLGSGLPTYIGIAVLLFSVRKGVFILSSQLLSFLITHGIKEWLNI
                     PRPSTLFNELGITLPQTVAGIDLHTWKSFPSGHTCAAFAFCFCVGVMIPKKYNYLKVL
                     LLILGISGAYSRIYLSQHFLEDTLAGSMIGVLSVIALYFPIYKNKWGEYPLFRFKKQK
                     K"
     gene            3392..4671
                     /gene="mtaB"
                     /locus_tag="PK641_02845"
                     /pseudo
     CDS             3392..4671
                     /gene="mtaB"
                     /locus_tag="PK641_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009292581.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
     gene            4807..7584
                     /locus_tag="PK641_02850"
     CDS             4807..7584
                     /locus_tag="PK641_02850"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPG55325.1"
                     /translation="MKRLLLLVSMTIASAVVFGQSIKGSVTDSQNGETLPGAYITIEG
                     TDNGTITDFDGTYSLDNLPSGQYTLIFSFLSYESKKISNIIVSNNKTTQVNASLDEAG
                     VQLEDIQIVGRKRTDTDLSLVKDMRTSLQVVSGISSQQISKSIDKDASEVVRRVSGTA
                     IQNDRFIIVRGLNQRYNNIWVNHAATPSSEIDSRAFSFDLIPSGLIDNLRIYKSSSPE
                     LPADFSGGFIQLTTKDNPSKTEFSINYGTTYRYGSSFEPFISNNISAADYFGFSAIGK
                     TLPTNFPKTLNNLSKANNSSATQSINKAWNTSKFTAPLDQNLSAYFQTSLIAKKTQIG
                     IISGINYGTDFTSYDITNKRFGIYQKKIDQEFARNDYKDTQYTNTSKLGATFNISFIF
                     DGENRLEFRNIFNQIGKTQVTKRTGFDNNNDYSIIETEYYNRSRSTFMSQLNGMHSFP
                     KFDGAINWTLGYVYTNCYEPDRRIITSRLNEDPNSIYNNKYRTEGNDVKRMYQYLDEN
                     ITSLSIDWIQDFKQQSWQPKLKAGIYTEYKNRNLNARNFIYQFNMNGLPNDFKYLPYT
                     EMFSESYIRADGVTLQENTNKSDSYQAENINASIYAALEMPIGDAAQIYAGVRLENNY
                     LRLFGYESDGIKPVNINRFTCTPFPPLNASYSINDQHKLRLSYGYAINRPEFREIVPY
                     VYYGFDLFSNFEGNPSLQDAYSHNADFRYEFYFPSGETISAAVFYKYFNHPIETTYYE
                     VGGQYQYTYTNAESAQNAGIEIEFRKSLDFMGLPDFQVVLNGSYIFSRIAFKDGSFER
                     NRAMQGQSPYLINASLFYNNEQIGLNASLQYNVAGERIVAIGVVNQDISEDIPNIYEM
                     PRHMLDFSISKTLGKYCTLKLGCKDMLNTACELKQFPQFEKDGKLQTREQIVRSYTPG
                     FQIQLGLSVKF"
     gene            7668..>7960
                     /locus_tag="PK641_02855"
     CDS             7668..>7960
                     /locus_tag="PK641_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008655785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55326.1"
                     /translation="MKKRMNLKMAFISAAIISLAACKNSNEPDQPKDPAQPSEKETVV
                     LQGEINGIRSLSADTTYLLKGFVYVTDGSKLNIEAGTIIKGDKDTKATLIVEKG"
BASE COUNT         2870 a         1359 c         1359 g         2372 t
ORIGIN      
        1 cagtatacag ggtttgaaaa caattctttt acataagcag ctccatctgt aggactaaat
       61 ggtcctgttt cgattcctgt tatttctaca aattgcattt taaaccaaaa gttatacaaa
      121 tgcaaaagca aaaatcctat tacaataaaa cctaatacta acatattttt tgacgcccaa
      181 tctacacctt cttgacgttc aactactgca tagcgttgtg ttccgcgagc acggtaattt
      241 tgtatagaca aaataaacgc atatacaaaa tgtactaata acaagaatgc taatccaaga
      301 gtacctatta gtgcatacca attagctcct aaaaaagcgc agattgcatt gtaaccttca
      361 ggcgaaaaca ccactacaac attcattgtt gcatggaaaa gtaggaacaa cactaatgaa
      421 gcacccgtga tactcatcac gaatttcctt cccacagaag aatcaaataa ccacatactt
      481 tttctgattt ttaaattatt aaataataga atggtaaata tcttaaaaag atgcgacaaa
      541 gttaataaaa cttttgtttt tatacaaaga aaatactact ttagccatcg cttttattaa
      601 caaacacaaa attattatga atcaattaaa aggtacacag acagagaaaa atcttgccat
      661 tgcttttgca ggagaaagtc aggctcgtaa tcgttatact ttttttgcca gcgtagctaa
      721 aaaagaaggt tttgaacaaa tatccgctaa ttttctagaa actgctgaac aagaaaaaga
      781 gcatgcaaaa aagttcttta aatacttaga aggaggtatg gtagaaataa cagcatccta
      841 ccctgcagga gttatcggga aaacaataga caatcttatg gcagctgcca acggcgaaag
      901 agaagaatgg agtaaagatt atcctcattt tgctgatata gccgaacaag aaggttttcc
      961 taaaattgcc gaaacgttta gaaaaattgc aactgtagaa cagatgcatg aagaacgcta
     1021 taaaaatttt attaaaaaca ttcaagaaaa caaggttttt gaaaaggatg aagaagttat
     1081 ttggcaatgc cgcaattgcg gatatctcca caaaggtaaa aaggctcctg aattatgccc
     1141 cgcttgcgag catccacaag cttattttga aattaaaaaa gaaaattact aaaacttttt
     1201 atagcagtaa acaaaaaaag gaatgtcaat cgacattctt ttttttgtaa ataagtacag
     1261 catttgcaaa aaaaatgtat atttgcagct gattgaacat ttttaagcaa tggattaaac
     1321 attttcgttc aaagtattga acatttttta aatatcagtt taaattttga caatatggaa
     1381 tataggcgat cacaatttaa agaaataatt aagagaataa acgaaactcg cagtaaaatg
     1441 caggttattg ttgggccacg tcaaattggg aaatccacct tgatagctca agtacttgaa
     1501 gaatgtccaa ttcctttcga tagttattct gcagatgata ttgtaggtgt atctgcagat
     1561 tggctttcac aaatatggga aacacaacga ctaaaaatat cggcaaacgc agaaagcaaa
     1621 agactattgg taattgacga agttcaaaaa ataaataatt ggagcgaaac ggtaaaggct
     1681 gaatgggatc gagataatag agaaaaactc aaccttatag tgatattgct cggttcatca
     1741 agaatgctta atgaaaaagg tttaacagaa tcacttgcag gaagatttga gcttattcgc
     1801 ttatctcatt ggtcatattc agaaatgaaa gactgttttg gttggtcttt gcaacaatac
     1861 atatattttg gtggttatcc gggtgccgct caatacatta acgacgagaa cagatggaga
     1921 aattatgtaa aagattcact aattgagaca tctatttcaa aagatgtttt aatggagagt
     1981 aaaataatga agccacaact attacgccag ctatttgaaa ttgggagcag ttatagcgga
     2041 gaattgttgt cattaacaaa aatagcagca caattacaag atgcgggaaa cgtgacaaca
     2101 ttagcaggat atcttcattt acttgacgaa tgtggattgc tatgcggatt acaaaaatat
     2161 gccgacgatt ttgctagacg atacaattct gttccaaaat ttcaggtaca taacagtgca
     2221 ttaagaaatg tttacgaaaa tgatgcattt tctgaagcaa ttattaatcc aaaaacatgg
     2281 ggaagatgtg ttgaatctgc tattggagcg tatttagtca gccaatctaa aattaaagac
     2341 tataaattat actattggag ggacagctca gatgaagttg attatatact cgttcaacgt
     2401 aacaaaataa ttgctattga agttaaaagt ggcagaagga cctctaataa gggcctatca
     2461 aaatttatca atttatatca tccgcacaaa gcctttgttg taggttcagg aggcttttcg
     2521 ttagaagagt tttttacttt agatctaagc atcttgttta aataatatga tattttaaaa
     2581 atccaattga acatagccct ttaccttatc ttaaattcaa tttgttatgc tttttgcgtt
     2641 tcgcacttct tatttgaatt ttcattagcc tttcatatca cacatttttg ctctatatca
     2701 agcttttttt gtaaatttgc cgatttaaaa atgatatgga acgattcaaa cagtttctgc
     2761 ataataacgc attattcttg tatccattta taatcctgtg gattataatg ttatgttttg
     2821 cattgatgca ttcaaaaact gaaactcact acttactaaa cacatggcat ggttcgtttg
     2881 gtgatgtgtt ttttcaatac gtaacttatt taggtagcgg attacccaca tacatcggaa
     2941 tagctgtgtt gctatttagc gtacgtaagg gtgtttttat actatcatca caactactta
     3001 gttttttaat tacacatgga attaaagaat ggctcaacat tccacgtcca agtaccttat
     3061 tcaacgaatt aggaataaca ctgccgcaaa cagtagccgg gattgactta cacacttgga
     3121 aaagttttcc atccggacat acttgtgctg cgtttgcctt ttgcttctgc gtaggtgtaa
     3181 tgatacccaa gaaatacaac tatttaaaag tattactact catattaggt atatcgggag
     3241 catattcgcg aatttatctt tcacaacatt ttttggaaga tacattagca ggatcaatga
     3301 taggtgtgtt atctgtgatt gcattgtatt ttccaatcta caagaataaa tggggcgaat
     3361 atccattatt tagatttaaa aagcagaaga aatgagcggc tttacggata aaacttttgc
     3421 attttacacc ttaggttgca aacttaattt tgccgaatca tcatcaatag gacgtacact
     3481 tatagaaatc ggatttcgca aagctaagaa cacagagcac cctgatattt gtatagttaa
     3541 tacctgttct gttacagatg gagcagataa aaaatgcaga caagccattc acaaaataca
     3601 ccgcgagcat ccaaatgcat accttgtggt tacaggttgt tatgctaaat aaaacctgat
     3661 gaaataatag taatagatgg cgttgatttg gttcttggag ccaacgacaa attcatattg
     3721 ccggacatac taaaccaaga aggcatcaac cgtatagtat cagacactcg aaaatcaacg
     3781 ccatttttct catcttgttc gtttgaagat agaacaaggc attttcttaa agtgcaagac
     3841 ggatgcgact atttttgtac gtattgcacc attccttttg cacgtggacg aagtagaaac
     3901 ggcagcatca aagatttata tgcagaagca aatcaagttg catcacaagg aggtaaagaa
     3961 attatactca ccggcgttaa tataggtgat tttggaaaaa ccacaggcga atcgtttttg
     4021 gatttgataa aagctcttga tgaaattgaa ggaattgaac gttttagaat ttcttctata
     4081 gaaccggatt tgcttacaga tgaaataatt aattttgtag ctaagtcaaa aagatttgcg
     4141 ccacattttc atattcctct tcaatcagga tgcgacgatg ttttaaaatt aatgcacaga
     4201 cattacgata cgacactttt tgctcataaa ataagactta taaaagaaat aattcctgat
     4261 gcgtttatag gagttgatgt aattgtaggt accagaggtg aaacacccga attttttgat
     4321 tcttcatata atttcattag ctcattggat gtttctgaat tacacatatt tagttattca
     4381 gaaagagcag gaactgccgc tttacgcatc ccattagttg taactgcaca agaaaaaaag
     4441 cgtagaagcc aacttttgct tgatttgtct gcggctaaaa taaacgactt ttacaaatca
     4501 caaataggca ctaaacgcag ggtactttgg gaagcatcaa ataaaaaagg atttatgttc
     4561 ggttttactg aaaactatct aaaattgaag cgtccattcg acaaagcata catcaacaaa
     4621 atagaagatg ttattgtaaa ctctgacaac atcacggaag taaatatcta ataaacggca
     4681 atacttatag tataatacca atctgtaact tttttgttac aacaaacaca caaaagctta
     4741 atactcttgt aatttgcatg aaacaccgca taggtacttt tgcacaagca tcataaagta
     4801 ctgcaaatga aaagattatt attgttagtg agtatgacga ttgcatcggc agtcgtattt
     4861 ggacaaagta ttaaaggctc agttacggat tcgcaaaatg gagagacact cccaggcgca
     4921 tatataacca ttgaagggac cgataacggt acaattacag attttgatgg aacatattca
     4981 ctagataact taccatcggg gcaatacact cttatttttt ctttcctgtc gtacgaatca
     5041 aagaaaataa gcaatatcat agtaagcaat aataagacaa ctcaagttaa tgcttcatta
     5101 gatgaagccg gtgtacaatt ggaagatata caaattgttg ggcgaaaacg aactgacacc
     5161 gatttgtctt tagtaaaaga tatgcgcaca tcacttcagg ttgtaagtgg tatttcttct
     5221 caacaaattt caaaatctat tgataaagat gcatctgagg tggtgcgcag agtttccgga
     5281 actgcaatac aaaacgacag attcattatt gttagaggtt taaatcagcg ctataataat
     5341 atatgggtta accatgccgc tacgccatcg agcgaaatcg acagccgagc tttctcgttc
     5401 gacttaattc caagcggatt aattgacaat ttacgaatat acaaatcttc atcacctgag
     5461 ctgcctgcgg atttttccgg cggatttatt caactcacca caaaagacaa tccgtctaaa
     5521 actgaatttt caataaatta tggtactact taccgatatg gtagcagttt tgaaccattt
     5581 atatctaaca atatatctgc tgccgattat tttggatttt cagcaattgg taaaactttg
     5641 ccaaccaatt tcccaaaaac acttaataac ttaagtaaag ctaataactc atctgccaca
     5701 caatcaatca ataaagcatg gaacacttcc aaattcactg ctcctctcga tcaaaatttg
     5761 tctgcatact ttcagacttc attaatagct aaaaaaactc aaattggaat tataagtggt
     5821 atcaattatg gtactgattt tacatcgtac gatatcacaa ataaacgatt cggaatatat
     5881 caaaaaaaga ttgatcaaga atttgctcgt aacgattata aagatacaca atacactaat
     5941 acgagcaaat taggagccac atttaatata tcattcattt ttgacggcga aaatcgtttg
     6001 gaattcagaa atatatttaa tcagataggt aaaacacagg ttaccaaacg tacagggttc
     6061 gacaataata atgattatag tattattgaa acagaatatt ataatcgtag ccgttcaacc
     6121 tttatgagtc agttaaatgg catgcactca tttccgaaat ttgatggagc aattaattgg
     6181 acattaggtt acgtttacac taattgttac gaaccggatc gaaggattat aacatcgcga
     6241 ttaaatgaag accccaacag tatttacaat aataaataca gaacagaggg caatgatgtt
     6301 aaacgaatgt accaatattt agacgagaat attacatcat taagtattga ttggatacaa
     6361 gacttcaaac aacaatcgtg gcagcccaaa ctcaaggcag gtatctatac cgaatataaa
     6421 aatcgaaacc ttaacgcacg taacttcata tatcaattta atatgaacgg attacccaat
     6481 gatttcaaat acctgcctta tacagaaatg ttttcggaaa gttatatacg agcagacggg
     6541 gttacattac aagaaaacac taataaatca gactcatatc aagcagaaaa cataaacgca
     6601 tcaatttatg cagcattaga aatgcccata ggagatgccg cacaaatata cgcaggagta
     6661 cgtttagaaa acaactatct acggttattc ggatatgaat ctgatggtat caaaccagta
     6721 aatattaaca gatttacatg tacccctttc ccaccattaa atgcaagtta ttcaattaac
     6781 gatcaacata aactacgatt atcgtacgga tacgcaataa atcgtcctga attcagagaa
     6841 attgtccctt acgtatatta cggctttgat ttgttttcaa atttcgaagg taacccatct
     6901 ttgcaagatg cttacagcca caacgctgac ttcagatatg aattttattt cccatcagga
     6961 gaaactattt ctgcagcagt attctataaa tactttaatc atcctatcga aacaacctat
     7021 tacgaggtag gcggacaata tcaatacact tatacaaatg ccgaatcggc acaaaatgca
     7081 ggtatcgaaa ttgaatttag aaagagtctt gattttatgg gattgcctga ttttcaagtt
     7141 gtattaaacg gatcatacat ttttagtcgt atcgcattta aagatggtag ttttgaacga
     7201 aacagagcta tgcaaggtca atcaccgtat ttaataaatg catcattatt ctataataac
     7261 gaacaaatag ggcttaacgc atcactgcaa tacaatgtag ccggggaacg tattgttgca
     7321 attggtgtag taaatcaaga tatcagtgaa gacattccta atatttacga aatgccaagg
     7381 catatgcttg attttagtat ctctaaaaca ctaggcaaat actgcacttt aaaactagga
     7441 tgtaaagaca tgttgaatac agcatgtgaa cttaaacaat ttcctcaatt cgaaaaagac
     7501 ggtaaattgc aaacacgcga acaaatagtt cgttcatata cacccggatt tcaaatacaa
     7561 ctcggtttaa gcgtaaaatt ttaacgacat tgtaatattt acgacataac atcttaatat
     7621 ataaacattt actttgtatc gtaattaata attataaaat tgaaaagatg aaaaaaagaa
     7681 tgaatttaaa gatggcattc atcagcgctg caattatcag tcttgcagct tgtaaaaaca
     7741 gtaatgaacc cgaccaacct aaagatccgg ctcaaccgtc tgaaaaagag actgtagtac
     7801 tacaaggaga aatcaatggc attcgctcat tatccgcaga tactacatat ctattaaaag
     7861 gttttgtgta tgttacagat ggttcaaagt taaatattga ggcaggtact ataattaaag
     7921 gtgataaaga tacaaaagca actttaattg ttgaaaaagg
//
LOCUS       DAOPID010000050         7959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_11541_length_7959_cov_3.993168, whole genome shotgun sequence.
ACCESSION   DAOPID010000050 DAOPID010000000
VERSION     DAOPID010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11541_length_7959_cov_3.993168"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="PK641_02860"
     CDS             complement(<1..812)
                     /locus_tag="PK641_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPG55327.1"
                     /translation="MEKFFRIHNYLIEHTQSPVPRKLMDDIDWNHRLIGIKGCRGVGK
                     TTFLLEYAKRFPEEKKECLYINLNQFYFTECNLVDFAGRFTAEGGKVLLIDQVFKYPD
                     WSSAIRQCYDLYPQLHIVFTGSSVMRLKEENPDLSGLVKVYYLQGYSFREYLNLIAGS
                     NIEPVRLDDILRNPNKFADAIKKQVDPSIHLQNYLHHGYYPFFLEKRNFSENLLKTMN
                     MMLEVDVLLIKQIAVDYLWKLRRLLYLLAISESSVTNVSQLSNDLNTSRATV"
     gene            complement(825..2021)
                     /gene="rho"
                     /locus_tag="PK641_02865"
                     /pseudo
     CDS             complement(825..2021)
                     /gene="rho"
                     /locus_tag="PK641_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005652729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
     gene            complement(2484..4043)
                     /locus_tag="PK641_02870"
     CDS             complement(2484..4043)
                     /locus_tag="PK641_02870"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPG55328.1"
                     /translation="MLPFQDIDIDAKAKLDYYLNNFGTHNCNYAFANLFSWRHKYHTS
                     WCIYNNCLIIKFLINGKNKIGYLQPIGKNIDPNIINIMIEDAASQNQPLRLYSMTKEF
                     TDIYKQTLVGKKSYIYQNRDFEDYFYTAESLRTLSGKNLQNKRNQINQFKKLYKNSSY
                     KLLNNTDKEEVENLMMLWGNEKALPDYGINEESDMIRQSFMHFDALGIFGIILYIDEH
                     AVGFSFGSYLNKKIFCVHAEKANTKYIGIYSYINQLLASNLPQDIELIDREEDLGIIG
                     LRKSKLSYYPEFLSSDYYAISKSDETEIWKLWKTCFGDSDEFIYSYLINYSKNDNRVL
                     KYQGDDLAAMLHFHIFKNRDIDKIAYLYGLATSPQYRKKGFACDVIEKSLQELWNNDV
                     KIVFLIQENKDFNSWYNKFDFTPYTESPLYFHSPDNFDFGTGIIEQNFGIYRILHALD
                     YLKIYAANHPLTNSTFYLSDTLFPKNDGTYKISNGIVEYQVMQTTDYIMHPQDIAKQF
                     PPDNVKLEFVM"
     gene            complement(4137..6506)
                     /locus_tag="PK641_02875"
     CDS             complement(4137..6506)
                     /locus_tag="PK641_02875"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPG55329.1"
                     /translation="MKKRNLFKKLLVGASLLFCAGFIQAQAPANAPDVILQGFYWDSY
                     GDDTYGTTKWTDLMTQVDELSANFSIVWLPPASSSDGGCGYHPKQWSNLSTSWGTKTS
                     LKNLIAALKTKGTRAMADIVINHRAGNFGWVDFCNEDFGTYGTFTLYESTQSNRYICS
                     DDEASGNGYTCTGAKDAGYDTQCSASGGYCAARDLDHSNTYVQNAVKAYLQWMKNEIG
                     YDGWRLDLVKGYLGKYTKAYNEAAGAYMSVGEYWDGDYNAVKNWINQTSKTSCAFDFP
                     MKYAALNNSLAENNYAGMASGYGVPQGLCGADEMKRYSVTFVDNHDTFRDTNKFGGDW
                     EAANAYILSAPGIPCVFYPHWVSCKEAIKKMIAARKACGVHSQSAASTAGTNSSYYKC
                     TTTGTKGTLICFIGSGWSAPQGYTLAGSGSKWAYYTSVQVPEGPTVTMSPNGGYVGPN
                     GQVTLSTTSGTIYYTTNGTTPSSGSTQYTSAITITTNNTTVKAIAIDGAEQSSIVSGT
                     FLTERPAGLTVSFKAPSTWNSVSLYAWTESNPKILGAWPGTVLTKSGDYYTYTITETE
                     VRPVNIIFNDNDNGHQTIDLSTSDDHCWDGSAGTGAIITPTTCDVEPSNITIKLKNHE
                     YFSTSNCHIVGADWPGATVALGQDGFYSINATATSLNVIFNNGGNGKQTTTISSETSI
                     CVQLTGETSQDEYSNTTYLWEETSCPGTAVDETIQSEVNIYPNPTSGIVSIQCDEEIA
                     NVIVRDMSANRIYEGNSSNFDISFASPAMYFVEIQLKSGQNVIKKLIKK"
     gene            complement(6637..7371)
                     /gene="truA"
                     /locus_tag="PK641_02880"
     CDS             complement(6637..7371)
                     /gene="truA"
                     /locus_tag="PK641_02880"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPG55330.1"
                     /translation="MPRYFIKLAYNGSNYHGWQIQPNGNSVQETIEKSMSILLGKPIA
                     IVGAGRTDAGVHAQEMIAHFNIENTIENTEELCYKLNLFLPKDIAVYSICIVKDDMHA
                     RFSARSRTYQYFVCTSKNVFKGNMSMRVPINTDFDAMNKAAKKLFDYIDFSSFSKQHT
                     DTKTNNCKIMQANWKQISEDTWVFTIQADRFLRNMVRAIVGTLLEVGSHKISIDEFCH
                     IIEQKNRCKAGTSVPAKALFLTKVEY"
     gene            complement(7379..>7959)
                     /locus_tag="PK641_02885"
     CDS             complement(7379..>7959)
                     /locus_tag="PK641_02885"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPG55331.1"
                     /translation="GSRIAKLAESLGFKVLQNDPPRQIAEKSSLFVPLSEIQNKADII
                     TFHVPLNNSTYHIVDDDFLSNCKRNPLIINAARGEVCDTATLLKCDNKIVLDCWENEP
                     TINIDLLKKVIIGTPHIAGYSIDGKANGTRIAATNICHFFGIKKTIIINLPPKDNMSY
                     NVKEESDKLKNQPDKFEFFRNNYPARRDIITF"
BASE COUNT         2318 a         1464 c         1343 g         2834 t
ORIGIN      
        1 attaccgttg cacgtgatgt atttaagtcg ttacttaatt ggcttacatt tgttacagaa
       61 gattctgaaa tggctaatag atatagtaat cttcttaatt tccacaaata gtctacagca
      121 atttgtttta tcaataaaac atctacctca agcatcatgt tcatagtctt gagcaaattt
      181 tcagagaaat ttctcttttc gagaaagaaa gggtagtatc cgtgatgcaa ataattttgt
      241 agatgtatac tcggatcaac ttgtttcttt attgcatctg caaatttgtt aggattccga
      301 agaatgtcat caagccttac cggttctatg tttgaacctg caatcaaatt aagatattcg
      361 cgaaatgaat atccttgtaa ataatatact tttactaagc ctgataaatc gggattctct
      421 tcttttagtc gcattacaga tgaacctgta aatactatat gcagttgcgg atataaatcg
      481 tagcattgcc gtatggcaga actccaatcc ggatatttga atacttggtc aataagtaaa
      541 actttgcctc cttcggctgt aaaccgcccg gcaaaatcaa ctaagttgca ttctgtaaaa
      601 tagaactgat taagattgat gtaaagacat tctttttttt cttcaggaaa cctttttgcg
      661 tattctaaca aaaaggtcgt tttaccaact cctctacatc ctttaatgcc aatgagtcgg
      721 tgattccaat caatatcgtc catcagtttc cgaggaacag gcgattgtgt gtgttcgatc
      781 aaataattat gtatcctgaa aaatttttcc aagaatttat gattttatcc gttcattgaa
      841 actaagaatt cttcattatt cttagttttt tccattctgt ctttcaaaaa ttccattgct
      901 tctacagaat tcatatcaga caaatgtctt cttaataccc acatacgact tagagtgttg
      961 gagtctaata gtaagtcgtc tctacgtgtt cctgacgcca taatatctat tgcagggaaa
     1021 atacgtttgt tagataattt gcggtctaac tgtaattcca tattacctgt tcccttaaac
     1081 tcttcaaaaa tcacatcgtc catcttagag ccggtttcta ttaaagcagt agcaataata
     1141 gttaaactgc ctccattttc aatatttcgt gcagctccaa agaaacgttt cggcttatgt
     1201 agtgcattag catcaacacc tccagataaa actttacctg atgcaggtgc tgttgtatta
     1261 tatgcgcgtg ccaatcttgt tattgaatca agcaaaataa caacatcatg tccgcattca
     1321 accaaacgtt tcgctttttc atgtacaata gaagctactt tcacgtgctt ctctgcaggt
     1381 tcatcgaatg ttgaagatac aacttcggct ctaacgctgc gtgccatgtc ggtaacttct
     1441 tcagggcgct catctataag taatataatc atgtacacct caggatgatt ctcaagtata
     1501 gcgttggcta attctttaag taatacagtt ttacctgttt ttggttgagc aacaatcaaa
     1561 ccgcgttgtc ctttacctat agggcagaat aaatcgacta tacgtgttga catcgaagta
     1621 ctcttgcctc cattcaattt gaatttttcg tcggggaata gtggagttaa atgttcaaat
     1681 ggtactctat ctcttacaaa tgaaggatcg cgcccattaa ttttatcaac tcttacaagt
     1741 ggaaagtatt tttcaccgtc tttaggcgga cgtatcggac cctcaacagt atcgcctgtt
     1801 tgtaaaccga ataaacgaat ttgcgattgg gatacatata tatcatcagg cgatgaaaaa
     1861 tagtcgtatt caggagaacg taaaaatccg tatccatctt gaactatttc aagtacaccg
     1921 ctacctttaa gtatatcgtc aaattcatat gctttttctt ctggtttatg gttgtgtttg
     1981 aattcagggc gtttgtgaga ttctttgttt tgctcgttgg atgtttctgg tacttctgtt
     2041 ggtgcagtag cgtcgattac ttcagactct ttagcaggag catctatagt ttcttctttt
     2101 tttcttgctt tttcttcact gcttttacgc aatttttctg ctattgtttc cataacgact
     2161 tcctcgtctg aaatcggaat agatggaata ggcggtgtat tatctgttat aacgtgtgat
     2221 tctgctacaa actcagtggc accttctatt tgtgttgctg ctgtggcaac tttttcgggc
     2281 atgcgtttaa tgcgtgtccg ttttttcttg acaacttctt cttcagccat aaaaatttaa
     2341 tgattttgca attgcaaaaa tgatcaataa gttatatata agttttggaa acattgttgg
     2401 cagtagttta gccaacgctt aacagcaaca aagatagtgt ttttttttga ataagcttat
     2461 caaatgatat ttttttatat cacttacata acaaattcaa gtttaacatt atcaggagga
     2521 aactgttttg caatatcttg tggatgcatt atatagtctg tggtttgcat tacttggtat
     2581 tcgactatgc cgttgctgat tttgtatgtt ccgtcgtttt taggaaataa agtgtctgat
     2641 aagtagaatg ttgaattagt taaaggatga ttagctgcat atatctttaa ataatctaaa
     2701 gcatgcagta ttctataaat tccaaaattt tgttctatta tgcctgtacc aaaatcaaaa
     2761 ttatcagggc tgtgaaaata taatggactt tcggtgtatg gagtaaaatc aaacttatta
     2821 taccatgaat taaaatcttt gttttcttgt atcaaaaata cgatttttac atcattattc
     2881 catagttctt gaagtgattt ttcaataaca tcgcatgcaa atcctttttt gcgatattgc
     2941 ggagatgttg ccaaaccata taagtaagct attttgtcta tgtcccggtt tttaaaaata
     3001 tggaaatgga gcatcgcagc taaatcgtct ccttgatatt ttaaaacgcg attatcattt
     3061 ttagaataat taataagata tgaatagatg aattcatcac tatcaccaaa acaagttttc
     3121 cataatttcc aaatttcagt ttcgtcgctc tttgaaatag catagtaatc gctagataaa
     3181 aactcgggat aataggataa tttagacttg cgtaacccga ttattcctaa atcttcttcg
     3241 cgatcaatga gttctatatc ttgcggtaga ttcgatgcta ataattggtt aatgtagctg
     3301 tatatgccaa tgtattttgt gttagctttt tctgcgtgta cgcaaaatat ctttttattc
     3361 aagtatgatc caaatgagaa tccaacggcg tgttcatcaa tataaagaat aattccgaat
     3421 atacctaagg catcgaaatg catgaatgat tgtcttatca tatcactttc ttcatttata
     3481 ccataatcgg ggagggcttt ttcatttccc cataacatca tcaaattctc gacttcttct
     3541 ttatcagtgt tattaagtaa tttgtagctt gaatttttgt ataatttttt aaactgatta
     3601 atttgattgc gcttattctg taagttttta ccactcaaag tacgtagtga ttctgctgtg
     3661 taaaaatagt cttcaaaatc cctattctga tatatataac ttttttttcc tactaatgtt
     3721 tgcttatata tatcggtaaa ttctttagtc attgaataca aacgcagtgg ttgattctgt
     3781 gacgcagcat cttcaatcat aatattgatt atattcggat ctatattttt accaattggt
     3841 tgcaagtatc ctattttgtt tttgccatta atcaaaaact ttataattaa gcaattgttg
     3901 tatatacacc agctagtgtg atatttatgt cgccatgaaa agagattcgc aaatgcatag
     3961 ttgcaattat gcgtaccaaa attatttaag tagtaatcta actttgcctt cgcatctata
     4021 tcaatatctt gaaatggcag catactaact atgttaatta ttttgcaaag ttacaaatta
     4081 aaacaagaaa aaggcggctt gccgaatgac aaaccgccta atctaattat aacaatttac
     4141 tttttaatta atttcttaat tacattttga cctgatttta attgtatctc tacaaaatac
     4201 atcgcaggtg atgcaaatga tatatcaaag ttgcttgagt tgccttcgta tattctattt
     4261 gcactcatat cgcgaacaat tacatttgca atttcttcat cgcattgaat agatacaata
     4321 cctgatgttg ggttagggta gatgttaact tcactttgaa tcgtttcgtc aacagctgtt
     4381 cccggacatg aagtctcttc ccacaagtag gttgtgtttg agtattcatc ttggctggtt
     4441 tctccggtta gctgtacaca aatacttgtc tcagaactta ttgttgtggt ttgttttcca
     4501 ttaccaccat tgttaaatat tacatttaac gatgttgctg ttgcatttat gctataaaat
     4561 ccatcttgcc ccaaagctac tgttgcacca ggccaatcag ctccaacaat atgacaattg
     4621 cttgttgaaa aatattcgtg atttttcaat ttaatggtaa tattactcgg ctcaacatca
     4681 catgttgtgg gcgttattat tgcacctgta cccgcactgc catcccagca atgatcatca
     4741 gaagtactta aatcaatagt ttgatgtccg ttgtcattgt cgttaaagat aatattaaca
     4801 gggcgtacct cagtttctgt aattgtatat gtgtaatagt ctccggattt ggtaagaact
     4861 gtaccaggcc atgctcctaa aattttagga ttagattctg tccatgcata taatgataca
     4921 gaattccacg ttgatggggc tttgaatgaa acggttaaac ctgcaggtct ctctgttaaa
     4981 aatgtaccac ttacaatact tgattgttca gcgccatcaa ttgcgatagc cttaacagtt
     5041 gtattgttag tagttattgt aatagctgaa gtatattgag tagagcctga tgatggagtt
     5101 gtaccatttg ttgtataata aatagtacct gaggttgtgc ttaatgttac ttgtccgtta
     5161 ggtcctacat atccgccgtt agggctcatt gtaacagtag gtccttctgg aacttgtaca
     5221 cttgtataat atgcccattt gcttccacta cctgctaaag tgtaaccttg aggagcagac
     5281 caaccgctac cgataaagca tataagcgta ccttttgtac ctgtagtagt acatttataa
     5341 taactactat ttgtacctgc tgttgaagca gcagattgac tatgaacacc gcatgcttta
     5401 cgtgctgcaa tcattttctt gatagcctct ttacaactaa cccagtgagg ataaaatacg
     5461 catggtatac cgggtgcaga aagaatatat gcatttgcag cttcccaatc tccaccgaat
     5521 ttatttgtat cgcggaatgt gtcgtggttg tcaacaaatg ttactgagta acgtttcatt
     5581 tcgtctgctc cgcataaacc ttgtggaacc ccatatccac ttgccattcc ggcgtaatta
     5641 ttttcagcta aagaattgtt taatgctgca tatttcattg ggaagtcgaa agcgcaagaa
     5701 gtcttgcttg tttggttaat ccaatttttt acagcattat aatctccgtc ccaatattcg
     5761 cccaccgaca tgtatgctcc ggcagcctcg ttatatgctt ttgtgtattt accaagatat
     5821 ccttttacaa ggtctagacg ccatccgtca tatcctattt cgtttttcat ccattgtaag
     5881 taagccttaa cagcgttttg tacataagtg tttgaatggt ctaagtcgcg cgctgcacaa
     5941 taacctcctg atgcactaca ttgtgtgtcg tatcctgcat ctttagcgcc tgtacaagta
     6001 tatccattac cgcttgcttc gtcatcgcta catatatacc gattactttg agtactttcg
     6061 tataaagtaa aagtaccata agtcccaaaa tcttcgttgc aaaaatccac ccaaccaaaa
     6121 tttccggcac ggtggttaat aactatgtcg gccattgcac gagtaccttt agtttttaat
     6181 gctgcaataa gattctttag acttgtttta gtaccccatg atgtgcttag gttactccat
     6241 tgttttgggt ggtatccgca accgccatca gaagaagaag caggtggtaa ccatacaata
     6301 ctaaaatttg ctgataactc atcaacttga gtcataaggt cagtccattt agttgttcca
     6361 taagtgtcgt ctccgtaaga atcccaatag aaaccctgaa gtataacatc aggagcattg
     6421 gcaggagctt gagcttgtat aaaaccggcg caaaacaaga gacttgcacc cacaagcaac
     6481 tttttgaaaa gatttctttt tttcattagt taatagtttt taaaaggtta gcaatatgtg
     6541 tattttgcaa acgcgtattg atcttatgcg cgctttttaa aaaaaaacaa tcatacaaat
     6601 ttactgcaaa tgtttcgaaa aacaaagatt gagatgttaa tattcaactt tagttaagaa
     6661 taaagctttg gccggtactg aagttcctgc tttacagcga tttttttgtt caattatatg
     6721 gcagaattcg tctatgctta ttttgtgact tcctacctct aatagtgtcc cgacaatagc
     6781 tcttaccata ttgcgtaaaa acctatctgc ttgtattgta aacacccaag tatcttcaga
     6841 tatttgtttc caattagcct gcattatttt gcaattattg gttttagtgt ctgtatgttg
     6901 tttactaaaa ctgctaaagt ctatataatc aaaaagtttt tttgcagcct tattcatggc
     6961 atcaaaatcg gtattaatag gtactctcat tgacatattt cctttgaata cgttttttga
     7021 agtgcataca aaatattgat atgtgcgaga tcttgcactg aatcttgcat gcatatcatc
     7081 ttttacaatg caaatggagt aaacggctat atctttaggt aaaaatagat taagtttata
     7141 acacaattct tcagtgtttt ctattgtatt ttcgatgttg aagtgcgcaa tcatttcttg
     7201 tgcatgtact ccggcatctg tacgtccggc tccaacaatt gcaatcggtt tgcctaataa
     7261 tatcgacata cttttttcaa tagtttcttg tacactattt ccgttcggct gaatttgcca
     7321 accatgatag ttagaaccat tgtaagctaa ttttatgaag tatcgaggca tatattgatt
     7381 aaaacgttat tatgtcgcgg cgtgcggggt agttattcct aaaaaactca aacttatccg
     7441 gttgattctt taatttatca ctttcttctt ttacattgta agacatattg tctttaggtg
     7501 gaagatttat aataattgtt tttttgattc caaagaaatg acaaatatta gtcgccgcta
     7561 ttcttgttcc gttggctttc ccatcaattg aataacctgc aatatgtgga gttccaataa
     7621 ttaccttctt tagtaagtca atatttatcg tcggctcgtt ttcccagcaa tcaagaacaa
     7681 tcttattgtc acattttaat aaagttgcag tatcacatac ttctcctctt gcagcattta
     7741 taataagtgg attgcgctta caattactta aaaaatcatc atcaacaatg tggtatgtag
     7801 aattatttaa aggtacgtga aacgtaataa tatcagcctt attttgtatc tctgataatg
     7861 gaacaaacaa agaagatttt tccgcaattt gtcttggtgg gtcgttttgc aatactttaa
     7921 atcctaagga ttctgctaac ttagcaattc tagaaccga
//
LOCUS       DAOPID010000051         7917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_11623_length_7917_cov_4.541465, whole genome shotgun sequence.
ACCESSION   DAOPID010000051 DAOPID010000000
VERSION     DAOPID010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 7917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7917
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11623_length_7917_cov_4.541465"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1146
                     /gene="murD"
                     /locus_tag="PK641_02890"
                     /pseudo
     CDS             1..1146
                     /gene="murD"
                     /locus_tag="PK641_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275190.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
     gene            1160..2398
                     /locus_tag="PK641_02895"
     CDS             1160..2398
                     /locus_tag="PK641_02895"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HPG55332.1"
                     /translation="MREFFKTIFRGDGVLWIMILLLFVISSLEMFSAISQVAYKGDNH
                     LAPFIGHVRYLALGAFVMLIFHHISYQKIRWFYLFLLPTSFVLLVITPFIGVEINGAV
                     RAIKIIGFEFQPIELAKWSVVLFLSDILARYQNHANKIPDKPFWWMIGVLAVICVLIA
                     MQNLSTAVILFGIGMIMMFVGRVGLGRISLVCAGALVVCMLIFGLSKVIPDQYLPDRM
                     KSGTWEKRITELFEENSQDASTKTYVIDDDNRQIMNSKIAIAKGKYPCGPGNSSQRDY
                     LPLAFSDFIYAILIEEYGIVGAALTMLLYLAILFRAGMIAYKSDTAYPAILVIGLSLL
                     IVVQAWISMAVTAHLGPVTGQPLPLISRGGTSILLTCIYLGIILSVSQYVKDIKKGNG
                     KNIVTASNDAENENPKTEEV"
     gene            2395..3483
                     /gene="murG"
                     /locus_tag="PK641_02900"
                     /pseudo
     CDS             2395..3483
                     /gene="murG"
                     /locus_tag="PK641_02900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
     gene            3622..4971
                     /gene="murC"
                     /locus_tag="PK641_02905"
     CDS             3622..4971
                     /gene="murC"
                     /locus_tag="PK641_02905"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HPG55333.1"
                     /translation="MGIGGIGMSAIARYFNKQGHNVGGYDLTSTDLTKSLEEEGIDIH
                     YSDNISLIPVKFMDPENTTVVYTPAIPNNMSEFVFFKEGKFNILKRSQVLGIITEGKH
                     SICVAGSHGKTTTSTMIAHILYQSKIGTNAFLGGISKNYDTNLLVTPNNNLVVVEADE
                     YDRSFLSLTPYMAVVTATDADHLDIYGSKEEMLKGFAEFTSLIQPEGVLIIKKGIELV
                     PAVQDGVKVYTYSATDESADFHAENVRIANSTILFDFKGINGIITDIELGVPVMVNIE
                     NAVAAIAIALLNGASTDEIKNGVKSFDGTKRRFDLKVKKENVTYIDDYAHHPNEIKAS
                     IASVRKLYPDKKICVVFQPHLYTRTRDFAAEFGESLSNLDDVILLDIYPARELPIKKV
                     TSELIYKQISGRHKTMCKKSELLEVLKTKEFDVLLTLGAGDIDKLVPEIQKFIEQKA"
     gene            4971..5732
                     /locus_tag="PK641_02910"
     CDS             4971..5732
                     /locus_tag="PK641_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55334.1"
                     /translation="MTDKTKYIIKIIASTITILAIIGYGIFAFVKFGNYGAQNVCNKI
                     QVKIVDDNDYKFIQPNDIITQLNECNIYPIRETISHKQTNQIEKCVEAMNLVKRAECY
                     KKNNGDVVVSVYQRHPMFLVVNGNNGYYIDTERKKMPTSSKFTAWLPVVSGFVSEKFA
                     KKDLYDFIVYINNDEFWSNHFGQIYINQKQEIELIAKNGVYNIYLGKLSNYQDKLKKL
                     RAWYEQYPEAAWGNKYSRVDLSYEKLIYCIKKTAL"
     gene            5729..6835
                     /locus_tag="PK641_02915"
     CDS             5729..6835
                     /locus_tag="PK641_02915"
                     /inference="COORDINATES: protein motif:HMM:NF018435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPG55335.1"
                     /translation="MKETYFAAIDLGTNKIAGAVAKKQEDGQLIIISLEEEATEANAV
                     LCGLVSDQQQILFQIKRLITKLQNSSKVTINNCYIGIGGKLFEEKYPKNMQLMNDVIN
                     SAKDLRPAINLIEVECPIICTANAVLSDHEKNNGCLLIDFGAGCTSFVLYENGEPIKK
                     NANRLGGDLVTKDITDLQLPFKIAEILKKKFGNALASTETPEKIIELSKKTDNGKVSS
                     IRSGQLAYIIEARVRETVDVLLNYLLSLNWFNKGIEKRSIVITGGASKLTNLDTLISE
                     KTKVSVRIANAGNKIANAKYFPTMQDARYSALCGMLLCATEECKIANEVIVKKKSKKH
                     PFKTVVDKIGQGFDNLFSGKNESFNETQENNTDK"
     gene            6856..>7917
                     /gene="ftsZ"
                     /locus_tag="PK641_02920"
     CDS             6856..>7917
                     /gene="ftsZ"
                     /locus_tag="PK641_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPG55336.1"
                     /translation="MINLNISNIQEQSIIKVIGVGGGGSNAVNHMYEEGIKDVSFLVC
                     NTDYQDLQRSPVPCKLQLGINTTKGLGCGANPEIGKMAAEESADEIKRILETSNTKMV
                     FITAGMGGGTGTGGAPVIASIAHDLGILTVGIVTIPFKFEMGKKIAKAMLGVAEMRKH
                     VDALLVINNERLIELYKDFSLANAFKEADNILTEAAKGIAEIITVQGYMNVDFADANT
                     ILKDGGVAIMNTGMAEGENRVINALKEALASPLLKCNDVRGAKRILINIYTSTTNEVI
                     IKEIEELKAFLNGINENLEEFIWGATYDDNLEETIKVTIIATGFELDNITGNIKEETK
                     QVETNTESNEKINKMVDEYY"
BASE COUNT         2877 a         1244 c         1516 g         2280 t
ORIGIN      
        1 gcagatgaaa taattaagag tccgggtatt cctgccaaag ctccgcttat ttaaaaatta
       61 aaagccctag aaactcctat catatctgaa atagaatttg caggcagata caatactgct
      121 aaaacagtat gcataacagg tagtaacgga aaaaccacta ccacaatgct tacatatcat
      181 attctacaaa aagcaggttt gaatgtaggc cttgccggaa atgttggaaa aagctttgca
      241 ttacaagtag ctgaaaacaa cttcgattat tacgtaattg aattaagtag ttttcaatta
      301 gataatatgt acgattttaa agccgatata gcggtgttac ttaatattac accagaccat
      361 ttggatagat acgaatacaa attccaaaat tatatagatg ctaaaatgcg tatcacaaga
      421 aatcagacaa aagacgatgt ctttatctat tggaacgatg acgaaaacat aaagaaagag
      481 ctatcaaaat tagttgtaaa tgccaaacaa catgcttttt ctcttgaaaa agcaccaaac
      541 acaaatgcct atgtcgagga tcaaaacatc actattaata cagaaaataa tcaattacaa
      601 atgaactcag acagcttatc tttaaaaggt acacacaata tatataactc tatggctgct
      661 gctctttctg ccagcgcatt atatattaaa aacgaataca tacgcgaatc tttaatggac
      721 tttgaaggag ttgaacacag attggaaccg gtagctcgcg tcaaaggtgc tatatatatc
      781 aacgattcaa aagctactaa tgtaaattct tgctggtacg ccttggaaag cataaaaact
      841 cctgttgtat taattttggg tgggactgat aaaggaaacg actacaacga gattttatct
      901 cttgtaaaag aaaaagttcg cgcacttgtg tttttaggaa aagacaatga aaaactagag
      961 aaattctttt acggagttaa gccttttgtt agtactgatt cgatggaaag tgctgtaaat
     1021 gaatgctatc gcttggcaga gaaaggagat acggtattac tatcaccttg ctgcgctagt
     1081 tttgatttat ttaaaaatta cgaagaccgt ggacgtatat ttaagcaatg cgtaaagaat
     1141 ttataattta agaaattata tgcgtgaatt ttttaaaaca atatttaggg gcgacggtgt
     1201 cttatggata atgattctct tgctgttcgt aatttcgtca ttagagatgt ttagtgccat
     1261 aagtcaggtt gcatacaagg gcgacaatca cttagctcca tttattgggc atgtgagata
     1321 tcttgcttta ggtgcttttg taatgttgat atttcaccat atatcatacc aaaaaattag
     1381 atggttttat ttatttttgc ttcctactag ttttgtatta ctggtgataa ctccttttat
     1441 tggagtcgaa ataaatggag ctgtaagagc tatcaaaata attggttttg aatttcagcc
     1501 cattgaactt gccaaatgga gcgtagtttt atttttatca gatatcttag cacgttatca
     1561 gaaccatgct aataagatcc ctgacaaacc attttggtgg atgattgggg tccttgctgt
     1621 tatttgcgtt ctcatagcaa tgcagaactt gtctactgcg gtaattctgt ttggtatagg
     1681 aatgattatg atgtttgttg gacgcgttgg attaggtcga atttctttag tttgcgccgg
     1741 agctttggta gtttgtatgt taatattcgg attatctaaa gtgatacctg atcagtattt
     1801 acccgatcgt atgaagagtg gaacttggga aaaacgaatt accgaattat ttgaagagaa
     1861 ttcgcaagat gcttcgacaa aaacctacgt aatagacgat gacaacagac agataatgaa
     1921 ttcaaaaatt gcaattgcaa aaggtaaata tccttgtggc ccgggtaata gtagtcaaag
     1981 agattacctt cctcttgcat tttctgattt tatatatgca attcttattg aagaatacgg
     2041 aattgttgga gctgctttaa ccatgctact atatcttgcc attttattta gagcaggtat
     2101 gatagcatat aaaagcgata cagcctaccc cgctattttg gttataggtt tgtctctact
     2161 tattgtagtg caagcatgga taagcatggc tgttacggct catctcggac ccgtaacagg
     2221 gcaacctcta ccattaataa gccggggtgg tacatcaata ttactgacct gtatttactt
     2281 aggaataatt cttagtgtca gccagtatgt aaaggatatt aaaaaaggaa acgggaaaaa
     2341 tatagttact gcatctaatg atgccgaaaa tgagaatcct aaaacagaag aagtatgagc
     2401 actaatacac ctgcaaactt tattatcagc ggtggcggaa ccggaggaca tatatttcca
     2461 gctatcagta tagcaaatgc tattaaaaaa tctatgcctg atgcaaatat tttatttgta
     2521 ggagcagaag gtagaatgga aatggaaaaa gtcccaaatg ccggatacaa aattgtggga
     2581 ttgcctattc agggattgta taggtcgttt acccgtaaaa acattaaggt tctgcgcaac
     2641 gcaattaaga gtgtaggtct atcaaagaaa atcattcaag actttaaacc tgatgcagta
     2701 attggtgtag gaggctacgc tagtggccct gtattgtggc aagcaggatc aatgaatatt
     2761 ccaactctta ttcaggaaca aaattcgtat gcaggtataa ccaataaatt attagggaaa
     2821 cgttgtaaaa aaatatgtgt tgcttacgac aacatggaac gctttttccc gaaaaataaa
     2881 attattatta ccggtaatcc tgtacgtcaa cagctattgg aaggcgttga taaacaacaa
     2941 gaagcattcc aatattttaa tttagatccg caaaaaaaga ctattcttgt aattggaggc
     3001 agccttggtg caggaacaat caacaaaagt gttttgcgca atatgaataa tctgactacc
     3061 acatacaaga atgatgttca ggttatttgg caaacgggta aatattacat caagcaaatt
     3121 cgtaatgaaa tagaaggata taacactcac aatatggtta ttaccgactt cgttacacgt
     3181 atggacttgg catattcaat agcagatctt gtaatatcac gcgcaggagc cagttctatt
     3241 tctgaattaa gtttactacg taaggcttgt attttagttc cttcacctaa tgttgctgaa
     3301 gaccatcaaa caaagaatgc gatggcttta gttgcaaaag aagcagctaa gatgattcct
     3361 gataacgaag cacaaatgcg actaatacct gaagcgatta aactgataaa cgataatgac
     3421 gaattattaa aactcagtca aaatattgaa tattttgcaa aacctgatgc agctcaacat
     3481 attgcaaaca caattttcga gatgattggt cgcgaaataa acacagctga agatgatgaa
     3541 tctgaagaaa aagaacttga aaagaaagta gaaaatgatt caaggcaatt ccaaaaaaca
     3601 caagctaaaa attatttctt cttgggtatc ggaggtatag gtatgagcgc cattgcccgc
     3661 tatttcaaca aacaaggaca taatgtcgga ggatacgatc ttacaagcac tgatttaact
     3721 aaatcgcttg aagaagaggg cattgacatt cattatagtg acaacataag tcttattcct
     3781 gtaaaattta tggatcctga gaatactact gtagtgtata cacctgctat ccctaacaat
     3841 atgagcgaat ttgttttctt taaagaaggc aagtttaata ttttgaaacg ctcgcaagta
     3901 ttaggaataa taacagaagg taaacactct atttgtgttg caggaagtca tgggaaaact
     3961 accacctcaa caatgattgc acatattttg taccaatcaa aaataggtac aaacgcattc
     4021 ttgggtggaa tatctaaaaa ttatgatact aaccttttgg ttacacctaa caataacttg
     4081 gttgttgttg aagctgatga atacgatcgt tccttcctta gcttgactcc atatatggca
     4141 gttgttactg cgacagatgc agatcatttg gatatatacg gcagcaaaga agaaatgcta
     4201 aaaggatttg ccgaattcac ttcgttaatt cagccggaag gtgtactaat tataaagaaa
     4261 ggcatagaac ttgtccctgc tgtacaagat ggggttaaag tttatacata ttctgcaaca
     4321 gacgaatctg ctgattttca tgcagaaaat gtacgaatcg cgaattctac tattttgttt
     4381 gattttaaag gaatcaatgg tataattaca gatatagaat taggagttcc tgtgatggtg
     4441 aatattgaaa atgcagtagc cgcaatagct atagcattac tgaatggagc atcaaccgat
     4501 gagattaaaa atggggttaa gagttttgat ggtaccaaac gccgtttcga cctaaaagta
     4561 aaaaaagaaa atgttactta cattgatgac tatgcgcatc accctaacga aataaaagca
     4621 agcattgctt ctgtaagaaa actctatcct gataaaaaaa tctgtgtagt attccaacca
     4681 catctataca cacgtacgcg tgactttgca gcagaatttg gagagagttt atctaattta
     4741 gatgatgtga ttttgcttga tatctacccg gctagagaat tacctatcaa aaaagttaca
     4801 tcagaattga tatataaaca gatatcaggt cgacacaaaa caatgtgcaa aaagtcggaa
     4861 ttgcttgaag ttttgaaaac aaaagaattt gacgtattac ttactcttgg agccggtgat
     4921 atcgataaat tggtacctga aatacaaaaa tttattgagc aaaaagcata atgacagaca
     4981 agactaaata tattatcaaa attattgcat ctaccataac aatacttgca ataataggat
     5041 atggtatatt tgcatttgta aaatttggaa attacggagc acaaaatgtt tgtaacaaaa
     5101 tacaagtaaa aatcgttgat gacaatgact ataagtttat tcaaccgaac gacattatta
     5161 ctcaactaaa tgaatgtaat atttatccta tccgtgaaac tatcagtcac aagcaaacta
     5221 atcagattga aaaatgcgta gaagcaatga acttggtaaa aagagctgag tgttataaaa
     5281 agaacaatgg cgatgttgtt gtaagtgttt atcaaaggca tcctatgttt ttagtcgtta
     5341 atggaaataa tggatactat attgatactg aacgtaaaaa gatgcctaca tcaagcaaat
     5401 ttacagcatg gttacccgta gtatccggat tcgtgtcaga aaaatttgca aaaaaagact
     5461 tatacgattt tattgtttac attaataatg atgaattttg gagtaatcat tttggacaaa
     5521 tttatattaa tcagaaacaa gaaatagagt taattgccaa aaatggtgtt tacaatatct
     5581 atctaggcaa actcagcaac tatcaagaca aattaaaaaa attacgtgct tggtacgaac
     5641 aatatccaga agcagcgtgg ggcaataaat acagccgcgt ggatttatct tatgaaaaat
     5701 taatttattg cataaaaaaa acagccttat gaaagagact tatttcgctg ccatagattt
     5761 aggtactaac aaaatagcag gtgccgtggc aaaaaagcaa gaggatggtc aattaataat
     5821 tatctcctta gaagaagagg caaccgaagc aaacgctgtt ttatgtggtc ttgtttcaga
     5881 tcaacaacaa attttatttc aaattaaacg actaattact aaactgcaga atagcagtaa
     5941 agtaactatt aacaactgct atataggtat cggtggcaag ttatttgagg aaaaataccc
     6001 caaaaacatg cagctaatga acgatgtaat taattctgca aaagatttga gaccagccat
     6061 aaatctaatt gaagtagaat gccctattat atgtacggca aatgctgttt taagcgatca
     6121 tgaaaaaaac aacggctgct tactaataga tttcggtgca ggatgcacat catttgttct
     6181 gtatgaaaat ggagaaccaa taaaaaagaa tgcaaatagg ttgggtggag atttagttac
     6241 caaagacata accgatctcc aattaccttt taaaatagct gaaatattga aaaaaaaatt
     6301 cggaaatgca ctagcatcaa cagagacgcc ggaaaaaatt attgaactat caaaaaaaac
     6361 tgataacgga aaagtatcta gcattaggag tggtcaatta gcttatatta ttgaagcacg
     6421 tgtacgcgaa acggttgatg ttttattaaa ttatttatta tcattaaatt ggtttaataa
     6481 gggaatcgaa aaaagaagta tcgttattac aggcggcgca tcaaaattga ctaatttaga
     6541 tactctaatt agcgaaaaga cgaaagtgag tgttagaatt gccaacgccg gaaacaaaat
     6601 agcaaacgct aagtattttc caactatgca agatgcccga tattctgccc tatgtgggat
     6661 gctattatgt gccacagaag aatgcaaaat cgcaaatgaa gttatagtta agaaaaaatc
     6721 taaaaaacat ccttttaaaa ctgttgttga taagattgga caaggatttg acaatttgtt
     6781 tagtggcaaa aatgaaagtt ttaacgagac acaggaaaac aatacagata aataacacat
     6841 aatataatgc cgagtatgat taacttaaac atttcaaaca tccaagaaca gtctataata
     6901 aaagtaattg gtgtaggcgg tggtggtagc aacgcagtaa atcacatgta tgaggaaggt
     6961 attaaagatg tatctttctt agtatgtaat accgactatc aggacttgca aagatcgcct
     7021 gtaccatgta aattacaatt aggcattaac acaacaaaag ggctaggctg tggggccaat
     7081 cctgaaatag gaaaaatggc tgcagaagaa agtgcagatg aaataaaaag aattcttgaa
     7141 accagcaata ctaaaatggt gtttatcacg gccggtatgg gaggcggcac cggaacagga
     7201 ggtgctcctg ttattgcaag tattgctcac gacttaggta tacttacagt tggtatagtt
     7261 accatcccat ttaaatttga aatgggcaag aaaattgcaa aagcaatgtt aggtgtagcc
     7321 gaaatgagaa agcacgttga tgccttattg gtgattaaca acgaaagact tattgaatta
     7381 tataaagatt tttcacttgc aaacgcattt aaagaagcag ataatatttt aactgaagct
     7441 gcaaaaggta tcgcagagat tattacggtt caaggatata tgaatgtcga ttttgctgat
     7501 gcaaatacta tcctgaaaga cggaggcgtt gctattatga atacgggtat ggcagagggc
     7561 gagaatcgtg ttatcaatgc attaaaagaa gctcttgcat ctccactatt aaaatgcaac
     7621 gatgtacgcg gtgctaaacg tatattaatc aatatataca cttcaacgac taacgaggta
     7681 ataattaaag agatagaaga attaaaagca ttcttaaatg gcatcaacga aaaccttgaa
     7741 gaatttattt ggggtgccac atacgacgat aatttagaag aaacaattaa ggtaacaatt
     7801 atcgcaaccg gtttcgaact tgacaatatt acaggcaaca ttaaagaaga aacaaagcaa
     7861 gttgaaacaa atactgaatc aaacgaaaag attaacaaaa tggttgatga atactac
//
LOCUS       DAOPID010000052         7753 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_11974_length_7753_cov_4.378800, whole genome shotgun sequence.
ACCESSION   DAOPID010000052 DAOPID010000000
VERSION     DAOPID010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 7753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7753
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11974_length_7753_cov_4.378800"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="PK641_02925"
     CDS             <1..739
                     /locus_tag="PK641_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55337.1"
                     /translation="LEEMTLNQSPDFKSNLVDYEYVGYGRDNGKENSAGGTGEQTGLI
                     YKKTRYVKQDQGRFFLSNTPQRPSKVSGASFNRMVTWVKLKERKSENVFYFFATHFDH
                     PTTQQGIDTRSAEADIALVQVPMIAGNSPLFFVGDFNCEPTEPAYSKLSAQWNDAFIE
                     IGDGAEGGYVCNEQQQANYPEACSEIGNTYTGLYSSSDKEPKRIDYILYTATMIDLFN
                     YIADNDNLGLVTYPSDHLPVITRMEFK"
     gene            complement(790..1104)
                     /locus_tag="PK641_02930"
     CDS             complement(790..1104)
                     /locus_tag="PK641_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HPG55338.1"
                     /translation="MNEFLSIEENIIRMLKTVFDPEIPVNIYDLGLIYKIDVDDDKNV
                     VIDMTLTAPGCPMADFITEDVKQKVQSIDTINEVTINIVFDPPWDKSMMSEEALLELG
                     YL"
     gene            complement(1129..1812)
                     /locus_tag="PK641_02935"
     CDS             complement(1129..1812)
                     /locus_tag="PK641_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HPG55339.1"
                     /translation="MKTYGLVNDGDKILIGISGGKDSLAMVELFANRMKIFKPKFSVE
                     AVHVSVSNIPYQADIEYLKQYCESFNVPFHHVITEFDASTDTRKSPCFLCSWNRRKML
                     FETAQKYNCNKIALGHHQDDILQTLLMNITFQGAFSTMPPLLKMNKMDVNIIRPMCLI
                     KESDLQKMADLRGFKKQIKNCPYEQDSHRNDIKRILKELEKINPDARHSLWGAMSNIQ
                     NEYLPQKKN"
     gene            1986..3845
                     /gene="mutA"
                     /locus_tag="PK641_02940"
     CDS             1986..3845
                     /gene="mutA"
                     /locus_tag="PK641_02940"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005682721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase small subunit"
                     /protein_id="HPG55340.1"
                     /translation="MAEKKLNFLADFPAISTQEWMDKITTDLKGADFTKKLVWRTSEG
                     FNVMPFYRAENLEGIQTTDSAPGKFPYVRGVKQNNVWAVCQNVDASSAKDANSKAREI
                     LNKGVDSLVIKLNKEELSAEYIKTLLDGIDASSVELNFTICIRKSAELFKLLIDYFKS
                     KKYDLSKLKGSVNCDPINRMLLKGKQLSFDKVSEIIKASVEASAELPLYRAVGVNAIS
                     LNNSGAYCAQELGYALSWGAQYMTMLTEAGVDSTEAGRNIKFKFGIGENYFMEIAKFR
                     AARMLWAKITEAYKPSCDCALKMNIHAETSRFNMTIYDANVNMLRSQTEAMSAALGGV
                     DSMTVTPYDVTFKTPDDFSERIARNQQLLLKEESHFDKVVDPAAGSYYLENLTAMIAE
                     QAWKLFIEIDNAGGFFAAVENSTVQKAIKDTLAKRQKDVASRKEVYLGTNQFPNFNEK
                     AATKIEATACACKCGCQVESVIEALPKVRAAEEFEELRLATEKAAKCPKAFMLTIGNL
                     AMRLARAQFSCNFFACAGYEVIDNIGFDTVEEGVAEAQKAGADIIVLCSSDDEYAEFA
                     PAAFKAIAGKQLFVVAGAPACMEDLQKAGIEHFINVKVNVLETLKSFNAKLLK"
     gene            3845..5971
                     /gene="scpA"
                     /locus_tag="PK641_02945"
     CDS             3845..5971
                     /gene="scpA"
                     /locus_tag="PK641_02945"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HPG55341.1"
                     /translation="MKPNFNNINIKEVAASKIDGDNESNWLTPELIPVKPVYSKEDIK
                     NLDFLNYASGLAPFLRGPYSGMYPMRPWTIRQYAGFSTAEESNAFYRRNLASGQKGLS
                     VAFDLATHRGYDADHERVVGDVGKAGVSICSIEDMKVLFNGIPLNKMSVSMTMNGAVL
                     PIMAFYINAGLEQGAKLEEMAGTIQNDILKEFMVRNTYIYPPKFSMKIIADIFEYTSK
                     NMPKFNSISISGYHMQEAGATADIELAYTLADGLEYLRAGVNAGMDIDSFAPRLSFFW
                     AIGTNHFMEIAKMRAARMLWAKIVKQFNPKNSKSLALRTHCQTSGWSLTEQDPFNNVG
                     RTCIEAMGAALGHTQSLHTNALDEAIALPTDFSARIARNTQIYIQEETKICKEIDPWA
                     GSYYVESLTNELAEKAWEHIQEIEKLGGMAAAIETGIPKMRIEEAAARTQARIDSGKQ
                     TIVGVNKYRLDHEDPIDILEVDNTAVRIQQIERLNQLRANRNEADVKKALDAITECVK
                     TGKGNLLELAVEAARVRASLGEISYACEQIVGRYKATIRTISGVYSSETNKDPEFQKA
                     CQLASEFAKKEGRQPRIMVAKMGQDGHDRGAKVVATGYADCGFDVDMGPLFQTPAEAA
                     KQAVENDVHVLGVSSLAAGHKTLVPQVIAELKKLGREDIVVIAGGVIPAQDYDYLYKA
                     GVAAIFGPGTSVAKAAVEIMKILLAD"
     gene            6171..6662
                     /locus_tag="PK641_02950"
     CDS             6171..6662
                     /locus_tag="PK641_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55342.1"
                     /translation="MKKLTILLAVAFLATTCFASSKSKANKDTQEWRYEIECAGTGSA
                     GTYLVKVWSYSKKQATATNQAVKNAVHGVLFKGIPGGNGCASQRPIASNPAIEYDRAD
                     FFERFFADGGDFQKYGSASGVPEIMKVGKEYKVGVVVSVYKDNLRKNLEQAGVIKGLG
                     SGF"
     gene            6713..7624
                     /locus_tag="PK641_02955"
     CDS             6713..7624
                     /locus_tag="PK641_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55343.1"
                     /translation="MKKIFATILALAMFAVSAFAEDVVYLNNGKVVKGNVIKDDGVNV
                     EVQTNSGVLTYKKLEIRKIDQSSVVVVPETPKRAKYIDYASQDKGWWCSVEVMGGGSV
                     DVNYDPALYPLGISFINGYRFNQFIEIGIGLGFRYYIPSTSIQNQTYYGINGLYEPKH
                     QQYSQTDNSGNTYVGAPWSIPLFIDLRGNLISNDTRTVVPYWAFDFGYSFSNYYLNGT
                     TATQNPLDPDDIIYDNSAAVASLGDGIFFAPTLGIKIGGPRNTLLLGISYMGQMLPRW
                     GGTIGQGNITSTTRYTSFMCGKIAYEF"
BASE COUNT         2558 a         1277 c         1508 g         2410 t
ORIGIN      
        1 actcgaagaa atgacattaa atcaatctcc cgattttaaa tcaaatttag ttgattacga
       61 gtatgtaggt tacggtcgcg acaacggaaa agaaaattca gcaggaggaa caggagaaca
      121 gacaggttta atatataaaa agactcgtta tgtaaaacaa gatcaaggac gattcttctt
      181 atcaaacaca cctcagagac catcaaaggt aagtggagcc tcatttaatc gtatggtaac
      241 atgggttaag ctaaaagaac gtaaatcaga aaatgtgttc tacttctttg caacccattt
      301 tgatcatcca acaacccagc aaggaataga tactcgttct gccgaggctg atattgcctt
      361 agttcaagta cctatgatag caggcaattc acctttgttt tttgtgggag attttaattg
      421 cgaacctaca gaacctgcat attcaaaatt atcggctcaa tggaatgatg cttttattga
      481 aataggagat ggggcagaag gcggatatgt atgtaatgaa caacagcaag ccaactatcc
      541 tgaagcatgc tcggaaatag gaaatactta tacaggtttg tacagtagtt ctgacaagga
      601 acctaaacgt atagactata tattatacac tgcaactatg attgatttgt ttaattatat
      661 tgcagataat gataatctgg gtttagtaac atatccatca gaccatttgc cggtaattac
      721 cagaatggaa tttaaataag tttactataa aaagaagttc gtggattaaa ttaggacgat
      781 aggctgtttt tataaatagc ctaattccaa tagtgcttct tcgctcatca tgcttttatc
      841 ccatggtggg tcaaaaacta tatttatggt cacctcatta atcgtgtcga tgctttgaac
      901 tttttgtttt acatcttcag ttataaaatc ggccataggg catcccggag ctgttagtgt
      961 catatcaata acaacatttt tatcgtcatc aacatctatt ttatagatta atcctaaatc
     1021 ataaatattt acaggaatct caggatcgaa aacggtcttg agcattctaa taatattttc
     1081 ttctatgctt aaaaattcat tcattgggaa ggtgtttatt agtgtttgtt aatttttctt
     1141 ttgaggcaaa tattcgtttt ggatattact cattgcaccc caaagactgt gtcttgcatc
     1201 agggtttatt ttctctaatt cttttaaaat acgttttata tcatttctat gcgaatcttg
     1261 ttcgtatggg cagttcttaa tttgcttttt aaagcctctt aaatctgcca tcttttgtaa
     1321 gtcagactcc ttaattaaac acatgggacg aataatatta acgtccatct tattcatttt
     1381 taaaagaggt ggcatagttg aaaacgcacc ctgaaatgtg atgttcatca ataaagtctg
     1441 taatatatca tcctgatgat gtcctaatgc aattttattg caattatatt tttgagctgt
     1501 ttcaaacaac atttttcgtc tgttccatga gcataaaaag caaggtgatt tgcgagtgtc
     1561 ggttgatgcg tcaaattctg ttataacatg atgaaaagga acattaaaac tttcacaata
     1621 ttgttttagg tactcaatat ctgcttgata tgggatattg cttacgctta catgtactgc
     1681 ttcaacacta aatttaggct taaaaatttt catcctattt gcaaataatt caaccatcgc
     1741 taaagaatct ttccctccgg aaataccgat gaggatttta tctccatcat taactagtcc
     1801 gtaggttttc atcgctttgc caaacttagc gtgcaaatga aataataatt tttgcgtatc
     1861 ggtcttgttt gtctgcatat cgaatacaaa agtagggaaa aaaatcgctt ttttaatgtc
     1921 ttgttataat aaaaaggcta actttgcgac ttgtaaaaat caattccatt aatttaataa
     1981 taatcatggc agaaaaaaaa ttaaactttt tggcagactt tcctgcgatt agtacgcaag
     2041 aatggatgga caaaattaca acagacctga aaggagcaga ctttacgaaa aagctcgttt
     2101 ggcgtacttc tgaaggcttt aatgtaatgc ctttttatcg tgcagaaaat ctagaaggta
     2161 ttcaaacaac agattctgca cccggaaaat ttccttatgt tcgcggagta aaacaaaaca
     2221 atgtttgggc tgtttgtcaa aatgttgatg catcatcagc taaagatgct aattcaaaag
     2281 ctcgtgaaat tttaaacaaa ggcgtagact ctttagttat caaactaaat aaagaagagt
     2341 taagcgcaga gtatattaaa acacttttag atggtattga tgcatcttca gtagaattga
     2401 attttactat ttgcattcgt aaatctgctg aattatttaa attacttatt gattatttca
     2461 aatctaaaaa atacgatttg tcaaaactta agggttcagt aaattgtgat cctattaaca
     2521 gaatgttgct taaaggaaaa caattatctt ttgacaaagt atccgaaatt ataaaagcat
     2581 cagttgaagc ttctgcagaa ttgcctttat atagagcagt cggagttaat gcaattagtc
     2641 ttaataactc aggtgcatat tgcgcacaag aattaggata tgcattatca tggggtgctc
     2701 aatatatgac aatgttgacc gaagccggag ttgattcaac tgaagcaggt agaaatatta
     2761 agtttaaatt cggtattggc gaaaattatt tcatggaaat agccaaattc cgtgctgcaa
     2821 gaatgttgtg ggcaaaaatt acagaagctt ataaaccatc ttgcgattgt gcattaaaaa
     2881 tgaatattca cgctgagaca tcacgtttca atatgactat ttatgatgca aatgtcaata
     2941 tgcttcgttc tcaaacagaa gcaatgagcg ctgcattagg tggagttgat tcaatgacag
     3001 ttaccccata tgatgttaca tttaaaacac ccgatgattt ttctgaaagg atagctcgta
     3061 atcaacaact attgcttaaa gaagaatctc atttcgataa agtagttgac ccagcagccg
     3121 gttcatatta cttagaaaat cttacagcaa tgattgctga acaagcatgg aaattgttta
     3181 tcgagataga taatgcaggc ggcttcttcg ccgctgttga aaattcaact gttcagaaag
     3241 caattaaaga tactttggca aaacgtcaaa aagatgtagc atctcgtaaa gaagtttatt
     3301 taggaactaa tcaatttcct aatttcaacg aaaaagcagc aactaaaatt gaagcaactg
     3361 catgtgcttg caaatgcgga tgccaagttg aaagcgtaat cgaagcattg ccaaaagttc
     3421 gcgctgctga agaatttgaa gaattacgtc ttgcaaccga aaaagctgct aaatgtccaa
     3481 aagcatttat gttgaccata ggaaatttag ctatgcgttt ggctcgtgct caattctctt
     3541 gcaatttctt tgcatgcgca ggatatgaag taattgataa tattggtttt gatacagtag
     3601 aagaaggtgt tgccgaagct caaaaagcag gcgcagatat tattgtatta tgttcaagtg
     3661 atgacgaata tgcggaattt gctcctgcag catttaaagc aatagcaggc aaacaattat
     3721 ttgtagtagc cggtgctcct gcatgtatgg aagatctgca aaaagcagga attgaacatt
     3781 ttattaatgt aaaagtaaat gtattggaaa cactgaaatc gtttaacgcc aaattattga
     3841 aataatgaaa ccaaacttta ataatatcaa tataaaagaa gtcgctgcat caaaaataga
     3901 tggcgacaat gagagtaact ggttgacccc ggagttaatt ccggtaaaac ctgtttattc
     3961 aaaagaagat attaagaatc ttgacttttt gaattatgct tccggattag ctcctttctt
     4021 gcgtgggcca tatagtggta tgtatccaat gcgtccttgg actattcgtc aatatgcagg
     4081 attttctaca gcagaagaat ctaatgcttt ttatcgtaga aacttagcat caggtcaaaa
     4141 aggactttca gttgcatttg acttagctac acaccgtggt tatgatgcag atcacgaacg
     4201 cgttgttggt gatgtaggaa aagccggtgt ttctatttgc tctattgaag atatgaaagt
     4261 gttgtttaat ggaattccat tgaacaaaat gtctgtttct atgacaatga atggtgcagt
     4321 acttccaatc atggcattct atataaatgc aggattagag caaggcgcca aattggaaga
     4381 aatggcagga acaattcaga atgatattct aaaagaattc atggtgcgta atacatatat
     4441 atatccacct aaattttcta tgaagattat cgccgatatt tttgagtaca cctcaaagaa
     4501 tatgcctaag ttcaattcaa tttcaatttc aggttatcac atgcaagaag caggtgcaac
     4561 agctgatatt gagttggctt acacattggc agatggtctt gagtatttac gtgcaggtgt
     4621 taatgccggt atggatattg attcatttgc tccacgtttg tcattcttct gggcaattgg
     4681 aacaaaccat tttatggaaa ttgctaaaat gcgtgcagct cgtatgcttt gggctaaaat
     4741 tgtaaaacaa ttcaatccaa aaaattctaa atcattagca ttacgtacac actgccaaac
     4801 atcaggttgg tcactaacag agcaagatcc gtttaataat gttggacgta cttgtatcga
     4861 agcaatggga gcagcattag gacatactca atctttgcat accaatgctt tggatgaggc
     4921 tattgctttg ccaaccgatt tttctgctcg tattgctaga aatacacaga tttatataca
     4981 agaagaaaca aaaatctgca aagaaattga tccatgggca ggttcatatt acgtagaatc
     5041 tttgactaac gagttggctg aaaaagcatg ggaacacatt caagaaattg agaaattagg
     5101 cgggatggct gcagctatcg aaacaggtat tccaaagatg cgtattgaag aagctgctgc
     5161 aagaactcaa gctcgtatag attcaggcaa acagactatt gttggtgtaa ataaatatcg
     5221 tttggatcac gaagatccga ttgatattct tgaagttgat aacacagctg ttcgtataca
     5281 acaaatcgaa cgtttaaatc aattgcgcgc aaatcgtaat gaggctgatg ttaagaaagc
     5341 attggatgca attaccgaat gcgttaagac aggcaaaggt aatttgttgg aattagctgt
     5401 tgaagcagca cgtgttcgtg catcattagg cgagatttca tatgcatgtg aacaaatagt
     5461 tggacgttat aaagcaacaa ttagaacaat atcaggcgtg tatagctcag aaacaaataa
     5521 agatcctgaa ttccagaaag cttgtcaatt agcttctgaa ttcgcaaaga aagaaggtcg
     5581 tcaacctcgt atcatggttg caaaaatggg acaagacgga cacgaccgtg gtgcaaaagt
     5641 agtagcaaca ggttatgctg attgcggttt cgacgtagat atgggacctt tgttccagac
     5701 tcctgcagaa gcagcaaaac aagctgttga aaatgacgta cacgtattag gtgtatcatc
     5761 cttagcagca ggtcacaaaa cacttgtacc tcaagttatt gctgaattga agaaactagg
     5821 ccgcgaagat atcgttgtaa tagcaggtgg tgtaataccg gcacaagatt atgactactt
     5881 gtacaaagcc ggtgttgctg caatatttgg ccccggaaca agtgttgcta aggctgcagt
     5941 tgaaataatg aaaattctgc ttgcagatta attcttatat ttagaaaaaa gaccgatgtt
     6001 agatcaaatt gacatcggtc ttttttttga ttgaacctga ttgttatttc taatgttaaa
     6061 acacattgtg ctttataaaa aaattaacca ttattatctt taagtaaaaa tttattttta
     6121 cctttacatt tattatttag catattacat ttttttaaaa tttataaata atgaaaaaac
     6181 tcacaattct tttagcagtt gcttttctgg caacaacatg ttttgcctct tcaaagtcta
     6241 aggcaaataa agatacacaa gaatggcgtt atgaaattga gtgtgcaggt acaggatctg
     6301 caggtacata tcttgtaaag gtttggagct attcaaaaaa acaggctaca gctaccaacc
     6361 aagcagtaaa gaatgctgtt catggtgttc tttttaaagg tatccccggt ggtaatggtt
     6421 gtgcatcgca gcgtcctatt gcatcaaatc ctgcaataga gtatgacaga gctgatttct
     6481 ttgaacgctt ctttgccgat ggcggagatt ttcaaaagta tggatctgca tcaggcgttc
     6541 ctgaaattat gaaagttggt aaagagtaca aggttggtgt ggttgtaagt gtatataaag
     6601 acaacttgag aaagaatctt gagcaagccg gcgttattaa aggcttgggt tccggtttct
     6661 gaaatactaa caattgggcg gtgtaatagc cgctctaaat aataataaac gtatgaaaaa
     6721 aatatttgct accattttag cacttgcaat gtttgccgtt tcagcatttg cagaagacgt
     6781 tgtatatctt aacaatggta aagttgttaa gggtaacgtt attaaagatg acggcgttaa
     6841 cgtagaggtt caaaccaata gtggagtctt gacctacaaa aagcttgaga ttagaaaaat
     6901 tgatcagtca tcagttgtag ttgttccaga aactccaaag cgtgcaaaat acattgatta
     6961 tgcaagtcaa gataagggtt ggtggtgctc ggttgaagtt atgggtggag gatctgttga
     7021 tgtgaattat gatccggctt tgtatcctct tggaatatca tttataaatg gatatcgttt
     7081 taatcaattt atagagatag gtattggtct cggttttaga tactacattc cttcaacaag
     7141 tattcaaaat caaacttatt atggaattaa tggcttgtat gagccaaaac atcaacaata
     7201 ttcacaaacc gataacagtg gcaacacata tgtaggagcc ccatggtcaa ttcctttatt
     7261 tattgattta cgtggtaacc tcatttccaa cgacacacgt actgttgttc catattgggc
     7321 atttgatttc ggttattctt ttagcaatta ttacttaaat ggcacaacag ccacacaaaa
     7381 cccattagat ccagacgata ttatatatga caatagtgca gcagttgcat cattaggtga
     7441 tggaattttc tttgcgccca cactaggtat aaaaattgga ggtccgcgca atacattatt
     7501 acttggcatc agctatatgg gtcaaatgtt accacgatgg ggtggaacta ttggacaagg
     7561 gaacattacc tcaactactc gttacacaag ctttatgtgt ggcaaaattg catatgaatt
     7621 ctaataaata aaatgtataa gcatatgaaa aagattattt tagcactttc aatgatttgt
     7681 gctgctctga cagtaaatgc cgccaattct accgaagctt attataattc agagaccgaa
     7741 tgtcttggag ttg
//
LOCUS       DAOPID010000053         7694 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_12113_length_7694_cov_4.439979, whole genome shotgun sequence.
ACCESSION   DAOPID010000053 DAOPID010000000
VERSION     DAOPID010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 7694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7694
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12113_length_7694_cov_4.439979"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /locus_tag="PK641_02960"
     CDS             <1..1110
                     /locus_tag="PK641_02960"
                     /inference="COORDINATES: protein motif:HMM:NF027798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,3-glucanase family protein"
                     /protein_id="HPG55344.1"
                     /translation="NAADGATQDWGDFNNYFKSNTYNKYVWFFHQSDVSTDRKSYAFA
                     YDDTFDQSATLYMNKPSKVRVTIGGFSNDPGGDVDPIETDDVAFDNLSNSIKSESSYT
                     VNVKYTATEARDIWICLFDNTGTWTVYPGGESHARVEAGSGVVPVTINLASAPTPAAN
                     YVFKCDIRPVDGDWTSAFDDEIISGITITGGGADEEDGESGTGKAGDLEYSYSFSQLG
                     TSLTVTLTVTNPGSFTGLVPVLFDKTDGFTEYVGTAGTIISKTFTYSLGKTTTFAGKW
                     MYAGGDVVSDDISYTMKLSTEASTREVSNIIVYPNPTNGLINIIGNDSLFLIYDSLGR
                     VCLQSVGQEADLSGLSKGTYYLRFADGTSLHLIKK"
     gene            1269..1700
                     /locus_tag="PK641_02965"
     CDS             1269..1700
                     /locus_tag="PK641_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55345.1"
                     /translation="MNKLFLSVLLTAISIASFAQLSPETIAKRERHKNLVVKEWNKDS
                     KGRNGWLDHLTTYDDQGRKIEEIEYANYGMRERVVFFYEESGNGKVTKEVVYDGRNKA
                     YRIRKYEYNVDGTKKKQYNYMPNGKLYSMKSFEYIFEDESK"
     gene            1697..2476
                     /locus_tag="PK641_02970"
     CDS             1697..2476
                     /locus_tag="PK641_02970"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HPG55346.1"
                     /translation="MISKESVLALPLWKDMVTIDLTEMKSVKLMNRVDTKFSAQLRLL
                     PDLLSRAISDYRVQVIDNSPVASYDTLYFDTPDLQMYLRHHDKILQRDKVRTRTYIDS
                     SLSFLEVKHKNNKGRTKKKRILISVDDFDDFTHNDEAINFLDNQVLYKIDALKPQVRT
                     VFNRITLVNNARTERLTIDFNLRFENEQTKLTAELPDVMIIELKQDGLVYSPMKKILS
                     DMNIHQIRISKYCIGTVLTNPNAKYNRFKKKVIFLNKLTKN"
     gene            2491..3219
                     /locus_tag="PK641_02975"
     CDS             2491..3219
                     /locus_tag="PK641_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HPG55347.1"
                     /translation="MNDFPEFDPTIAEEFGTTGADATAALDFLGVPLFDGVSLTQLLI
                     RFAFNLLMCWIIVRFFYYKKSRRRDYYATFLLFNTTMFLLIVLMENVNMQIGLTLGLF
                     AIFGMIRYRTETVPIREMTYLFVITGMAVINGLAMAVSYAELVCANLLFVLVIAFVEK
                     QRFLKHTSTKLILYERIELIVPERRAELIADIEKRIGFKIEKLEIGHIDFLRDVAFIK
                     LYYELGKGENNTIDTLTKTNQFVG"
     gene            3216..3989
                     /locus_tag="PK641_02980"
     CDS             3216..3989
                     /locus_tag="PK641_02980"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HPG55348.1"
                     /translation="MKRNSLVYIFLLMCLTPIMAQTIEYADEESSTEARLRVGADFQY
                     NPVKGLSLSLEEEARMRNNFCTFDRLYSTFDISYRVNSYFKFGGAYSFMILYQDGRKK
                     TDYVSYWDFRHRAVAHATGSISFNRFTLSLRERFQTTFRTDSLYNENEVVNPKMYLRT
                     RLKLEYDFFSKPLKPYLSVEMFNPLNQTKYTNQWIDEMQYRLGLEWRLDAFNTLEFFY
                     MFDHSFGNDVDVKSKKDKVIITPETEYNHIIGVFYSIRF"
     gene            4013..4237
                     /locus_tag="PK641_02985"
     CDS             4013..4237
                     /locus_tag="PK641_02985"
                     /inference="COORDINATES: protein motif:HMM:NF024672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HPG55349.1"
                     /translation="MKKIDFKIREGETYIPLIQLLKSANVVCSGGEAQQVVVDGLVLR
                     NGVVELRKRAKIISGDVILFQDFEIVVRNA"
     gene            complement(4323..5984)
                     /locus_tag="PK641_02990"
     CDS             complement(4323..5984)
                     /locus_tag="PK641_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HPG55350.1"
                     /translation="MEWLINLLTNESIAHTLLLYSFIIAFGVFLGKVKIFGISLGVTF
                     VLFVGIIVGHFGFTVNSTVLEFIKEFGLILFIYTIGLQVGPGFFSSFKKGGMVLNTLA
                     CGIVILGIITAISIYYIANDRIPFPMIVGIMSGAVTNTPGLGAAEEALRQIHYTGSSI
                     GLGYAVAYPLGVVGIILSMVLIRVLFRIKLDNESEKLISQDDNAKEVPERMTLKITNK
                     AIEGKTMKECRNLVGRDFIISRMIENDTIVIPAGNTVLHTGNIILVVTTSHNAESVRT
                     FLGEEIEYKWEDDDSKLVSRRIVITQDSINGKTIGSLKLRSVLGVNITRVNRSGIDLL
                     ATPSLILQVGDRVMVVGPLDAIKRAEKILGNTLKRLNEPHIISIFIGILTGILVGSIP
                     FYFPGMPMPAKLGLAGGPLIVAILVGRFGYKIKLITFTTQSANLMLREIGICLFLASV
                     GLSSGSEFFKTVLSGDGLLWIGCGFLITIIPAIIIGIISRSCFKLNYYTIMGLIAGSM
                     TDPPALAYASSTANNDAPAIAYSTVYPLTMFLRIISAQVLILAFA"
     gene            complement(6108..>7694)
                     /locus_tag="PK641_02995"
     CDS             complement(6108..>7694)
                     /locus_tag="PK641_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPG55351.1"
                     /translation="YGIGNPEDFTKNMVNIRTGQKFVRDEFLRHLVSSMYTRNEIDFQ
                     RGNFRVKGDTVDIWLAYTEIVCRVLFWGDEIDSIELLEPISMSTIESLDEYSISPANI
                     FVTTKENTFRAIGEIEIDLGKQVDYLKSIGKNYEAKRLYERTTYDIEMIRELGYCSGI
                     ENYSRYFDGRNAGTPPFCLMDFFPKDFIMVIDESHVTIPQIHAMYGGDKARKKNLVEY
                     GFRLPAAMDNRPLTFEEFEGKEKQTIYVSATPAEYELQKSGGIITEQVIRPTGLLDPE
                     IIVKPSKNQIDDLLEEITIRAEKDERVLVTTLTKRMAEELTEYLLKNGVRCTYIHSDV
                     DTLERVQILDDLRAGLYDVLIGVNLLREGLDLPQVSLVAILDADKEGFLRSHRALTQT
                     AGRAARNLDGKVIMYADTITESMQKTIDETIRRRNKQNEYNKQNNITPTAILRDKHSP
                     LAKISNSPIRENSAYMEPTELNIAADPIVKYMTKDQLLKAAEKAKKSMLAASKEMDYI
                     QAAQYRDEMMKLQDLAAKTK"
BASE COUNT         2493 a         1266 c         1375 g         2560 t
ORIGIN      
        1 aatgctgcag atggagcaac ccaagattgg ggcgatttta ataattattt taaatcaaat
       61 acatataaca agtacgtatg gttctttcat caatctgatg tgagcacaga tagaaagagt
      121 tatgcctttg cttatgatga tacatttgat caatctgcaa cattgtatat gaataaacca
      181 tctaaggtta gggtaacaat aggaggattt tcaaatgatc ctggtggtga cgtagatcct
      241 attgaaacgg atgatgtagc attcgataat ttatcaaatt ctattaaaag tgaatcaagc
      301 tacactgtaa atgttaaata tactgctacc gaggctcgtg atatatggat ttgtctattt
      361 gataacacag gcacatggac tgtatatccc ggaggtgaat cacatgcaag agttgaagcc
      421 ggttcaggag tcgttcctgt tacaattaat ttagcctcag cacctacacc ggcagcaaat
      481 tatgtattta aatgtgatat tcgtcctgta gacggagact ggactagtgc atttgatgat
      541 gaaattatat ctggtataac aataacaggc ggtggagctg atgaagaaga tggagaaagc
      601 ggtacaggca aagccggaga tttggaatat agttatagct tctcacaatt agggacttct
      661 ttgacggtta cacttaccgt aacaaatcca gggtcattta caggtttggt accggtgctt
      721 tttgataaga cagatggctt tacagagtat gttggtacgg caggtactat aattagtaag
      781 acgtttacat attctctagg aaaaacaact acattcgcag gaaaatggat gtatgcagga
      841 ggcgatgtgg tttccgatga tatttcatat actatgaaat tgtcgacaga agcatctacc
      901 cgagaagtat caaatattat agtttatccg aatcctacta atggcttaat taacattatt
      961 ggcaatgatt ctttgttttt aatatatgat tctttaggac gcgtatgttt gcaatcagtc
     1021 ggacaagaag ctgatttatc aggcttatca aagggaacat attacttacg ttttgcagat
     1081 ggtacaagtt tgcatttaat taagaaataa caaaatagtt gatataaaag tttttattaa
     1141 taaagcgttt cgagtctatt tgattttgaa acgctttatt aatatgtttc attatatagt
     1201 tttttttgaa taaaaaaata tttatcttta cagattaata attataaaca taaccttaga
     1261 tttaatatat gaataaatta ttcttgtcag ttcttctgac tgcaatttcg attgcgtcct
     1321 tcgcacaact ttctcctgaa acgatagcaa aaagagaacg tcacaaaaat ttagttgtaa
     1381 aagagtggaa taaagattca aaaggcagaa atggttggct tgatcacctt actacttacg
     1441 atgatcaggg tagaaaaata gaagaaattg agtatgccaa ttatggtatg cgcgagcgtg
     1501 ttgtgttctt ttatgaagaa tcagggaatg gaaaagtaac taaagaagtt gtatatgatg
     1561 gtcgaaacaa ggcatatcgt attcgtaagt atgagtataa tgtcgatggt actaagaaaa
     1621 aacagtacaa ttatatgcct aatggcaaat tatactcaat gaaaagtttt gaatatattt
     1681 ttgaagacga atccaaatga taagtaaaga atcagtatta gctttaccct tgtggaaaga
     1741 tatggttacc attgatttga cagaaatgaa atccgttaaa ttaatgaata gagtcgatac
     1801 aaaattctca gcacagctta gacttttgcc tgatttacta tcaagagcta tttctgatta
     1861 ccgtgtacaa gtaattgata attcgcctgt tgcatcatac gatactcttt attttgatac
     1921 tcctgattta caaatgtatc taagacatca tgataaaata ttgcaacggg ataaagtacg
     1981 aacacgtaca tacattgatt cgtctttatc ttttttagag gttaagcata aaaataataa
     2041 gggacgtaca aagaaaaaaa ggattttaat atcagttgat gattttgatg attttacaca
     2101 taatgatgaa gctattaatt tcttagacaa tcaggtgcta tataaaatag atgcactgaa
     2161 acctcaagtg cgtactgtct ttaatagaat aacattagta aataatgcaa gaacagaacg
     2221 attgactatc gattttaatt tgcgttttga aaatgaacaa acaaaactta cagccgagtt
     2281 gcctgatgta atgattattg aattaaaaca agatggttta gtatattcgc ccatgaaaaa
     2341 aattttgtct gatatgaaca ttcatcaaat tagaataagt aagtattgca taggaactgt
     2401 attgactaat cctaatgcaa aatacaatag atttaaaaag aaagtgattt ttctaaataa
     2461 attaaccaag aattaaatac aattacgatt atgaatgatt tccctgaatt tgatcctacc
     2521 attgcagaag aatttggcac aaccggtgca gatgcaactg cagcattaga ttttttagga
     2581 gtaccattgt ttgacggagt aagcttaact caattgttga tacgttttgc attcaacttg
     2641 ctaatgtgtt ggatcatagt gcgtttcttc tattataaaa agagccgtcg cagagattat
     2701 tatgcaacat ttttgttgtt taacacaact atgttcttac taattgtgtt aatggaaaat
     2761 gttaatatgc aaatcggtct tacacttggt ttgtttgcta tttttggaat gatacgatat
     2821 cgaacggaaa ctgttcctat tcgtgaaatg acatatctat ttgtaattac cggtatggct
     2881 gtaattaatg gtttggcaat ggccgttagt tatgctgaat tggtttgtgc aaaccttcta
     2941 tttgtattgg ttattgcgtt tgtagaaaag caacgctttt taaaacatac ttcaacaaaa
     3001 cttattcttt acgaaagaat tgaacttata gttcccgaaa gacgtgcaga attaatagct
     3061 gatatcgaaa aacgtattgg ctttaaaatt gaaaagttgg aaataggtca cattgatttc
     3121 ttgcgtgatg tagcttttat caaattatat tacgaactag ggaaaggtga aaataacact
     3181 attgatactt tgacaaaaac gaatcaattc gttggatgaa gcgaaatagt ttagtatata
     3241 tatttttact gatgtgccta acgcctatta tggcgcaaac catagaatat gcagatgaag
     3301 agtcttctac agaggctaga ttgcgtgtag gtgcagattt ccaatacaac cctgtaaaag
     3361 gattgagttt gagtttggaa gaagaagcca gaatgcgtaa taatttttgt acttttgaca
     3421 gactctactc tacctttgat atttcgtata gagtgaactc ttacttcaaa ttcggaggag
     3481 catattcgtt catgatttta tatcaagacg gtaggaaaaa gactgattat gtatcatatt
     3541 gggattttag acacagagca gtagctcatg ccacaggttc tatatctttt aatcgtttta
     3601 cacttagtct gcgtgagcgt tttcaaacaa cttttagaac cgattcatta tataatgaaa
     3661 acgaggttgt gaaccctaaa atgtatttgc gaactcgctt gaaattagaa tatgattttt
     3721 tttctaagcc tttaaagcca tatctatctg tagaaatgtt taacccttta aatcaaacca
     3781 aatatactaa tcaatggatt gatgaaatgc agtacagatt aggtttggaa tggcgattag
     3841 atgcattcaa tacattggag ttcttctata tgtttgatca cagcttcgga aatgatgtag
     3901 atgtgaaatc aaaaaaagat aaggttataa taactcctga aactgaatat aatcacatta
     3961 taggggtctt ctattctata cgtttttgat tttactaagt ttttttgatt acatgaaaaa
     4021 gatagatttt aaaattcgtg agggtgaaac ctatattcct ctaattcagt tgcttaaatc
     4081 agcaaatgtt gtgtgttcag gcggagaggc tcagcaagtt gttgtcgatg ggcttgtact
     4141 ccgaaatgga gtagtcgaat tgcgcaagcg tgcaaaaata atcagtggtg atgtaatctt
     4201 atttcaagat ttcgaaatcg tagttcgtaa tgcttaagtt taggcgcaaa ttaataatta
     4261 agcaaagcta tttaatgatt tttgcttaag ctaataaaca ttctaaatta aattcactgc
     4321 tactacgcaa atgctaatat taacacctgt gcagatataa tccttaaaaa catcgttagt
     4381 ggataaacag tagagtatgc aatagcaggc gcatcgttat tagctgttga acttgcatac
     4441 gcgagtgccg gaggatcggt catactgcca gcaatcaaac ccatgatagt gtagtagtta
     4501 agtttaaagc aagatctaga tataatacca attataattg cgggaataat ggtgattaaa
     4561 aatccgcatc ctatccaaag caagccatca cccgataaaa ccgttttaaa aaattcactg
     4621 cctgacgaca gacctacact tgctaagaat aagcatatac ctatttctcg aagcatcaga
     4681 tttgcacttt gtgttgtaaa tgtaatgagc tttattttgt aaccaaatct tcctactaaa
     4741 atagcaacaa tcagcggacc tcctgctaaa cctaattttg ccggcattgg cattccgggg
     4801 aaatagaatg gtatactacc aaccaagata ccggttagaa taccaataaa aattgatatg
     4861 atatggggct cattaagtct tttaagagta tttcctagaa ttttttctgc tcttttaatt
     4921 gcatctagtg gccctacaac cataacacgg tcaccaactt gaagaatcaa acttggtgta
     4981 gcaagcaagt ctatgccgga acggttgaca cgtgtaatat taactcccaa tacgcttctt
     5041 aattttagag aaccgattgt tttcccgttt atactatctt gtgtgataac tatgcggcga
     5101 gataccaatt ttgaatcgtc atcttcccat ttgtattcaa tttcttctcc gaggaatgta
     5161 cgtaccgatt ctgcgttatg acttgtagtt actactaaga ttatattacc ggtatgtaat
     5221 actgtatttc cggcaggaat aacaattgtg tcattttcta tcattcttga tataataaaa
     5281 tcgcgaccca ctaggtttct gcattccttc atcgttttcc cttcaattgc cttatttgta
     5341 attttcaaag tcatgcgttc cggtacttct ttagcattgt catcttgcga tattaatttt
     5401 tcactttcgt tatcaagttt gattcggaat aaaacacgta taagtaccat tgataaaata
     5461 attccaacaa ctccaagagg ataagctaca gcatatccca aaccgataga gctaccagtg
     5521 taatgtattt gacgaagtgc ttcttcagct gcacccaaac ccggcgtatt tgttacagct
     5581 cccgacatta tgccaacaat cattgggaaa ggaatgcggt catttgcaat gtaatagatt
     5641 gagattgctg taattatgcc tagtataaca atgccgcaag ctaaggtatt aagcaccatt
     5701 cctccttttt tgaaagaaga aaaaaatcca gggcctactt gcaagccaat tgtgtatata
     5761 aataaaatga gtccgaattc tttaataaat tccaaaacgg tagaattgac tgtaaagccg
     5821 aaatgtccga ctattatgcc aacaaataat acaaaagtaa cacctaatga tatgccaaat
     5881 atttttactt tgccgagaaa aaccccaaat gcgattatga atgaatacag caacaatgta
     5941 tgagcaatag actcattggt taaaagattt ataagccatt ccataaaaga aattttttat
     6001 aatttagtta ttaaagtgtt attgtaaatt gcaattacag tgtactttgc ggcatcaaag
     6061 gtatgctttt tttctttgaa agcaatatgt agagatacta aatattatta ctttgtttta
     6121 gctgccaaat cttgtaattt catcatttca tctcggtact gagcagcttg aatgtaatcc
     6181 atctcctttg atgcggcaag catagatttt ttagcttttt ctgcagcttt gagaagttga
     6241 tcttttgtca tgtatttaac aataggatct gctgcaatat ttagttcagt tggctccata
     6301 taagcactat tttctctaat cggcgaattt gagattttag ccaaaggaga atgtttgtct
     6361 cttaaaatag cagttggagt aatattattt tgtttattat attcgttttg cttatttctg
     6421 cggcgtattg tctcgtctat tgttttttgc atgctttctg taattgtatc agcatacata
     6481 ataactttcc cgtctaaatt acgtgcggct ctaccggctg tttgggttaa agctctatgt
     6541 gagcgcaaaa atccctcttt gtcggcatct aatattgcaa ctaatgatac ttgaggtaag
     6601 tcaagacctt cgcgcaataa atttacgcca attaagacat cgtataatcc tgctcgtaaa
     6661 tcgtctaaaa tttgaactct ttcaagagta tctacatcag agtgaatgta agtgcatctg
     6721 actccgtttt taagcaggta ttctgttagc tcttctgcca ttcttttggt tagagtagtt
     6781 accaaaactc tttcatcctt ttcggcacgt attgtaattt cttcaagcaa gtcgtcgatt
     6841 tgattcttgc taggcttaac aatgatttca gggtctaata aaccggttgg tctaatgact
     6901 tgttcggtta ttattcctcc ggatttctgt aattcatatt cagcaggggt tgcacttaca
     6961 taaattgtct gtttttcttt tccctcaaac tcttcaaatg taagaggacg attatccata
     7021 gctgcgggta atctgaaacc atattcaacc aaattctttt ttctagcctt atcaccgcca
     7081 tacattgcgt gtatttgagg aatagtaaca tggctttcat cgataaccat tataaaatct
     7141 ttaggaaaga agtccatcaa gcaaaatgga ggagtccctg cattacggcc atcaaaatac
     7201 cttgaataat tctcaatgcc tgaacaatat cctaattcac gtatcatttc aatgtcgtaa
     7261 gtagtacgct cgtatagacg tttggcttcg taattctttc ctatggattt taagtaatca
     7321 acttgtttac ctaggtctat ttcaatttct ccaatggcac gaaatgtatt ttcttttgta
     7381 gtaacaaaaa tattggcagg tgatatgctg tactcatcta acgattctat agtagacatg
     7441 ctgataggtt ctaataattc aatgctatca atttcatcac cccaaaataa taccctgcaa
     7501 acaatttcag tatatgctaa ccaaatatca acagtatcgc cctttaccct aaaatttccg
     7561 cgttgaaaat cgatttcatt acgagtgtac attgaagata caagatgtcg caaaaattca
     7621 tctcttacaa atttttgtcc cgttcttata tttaccatgt tcttggtaaa atcttccgga
     7681 tttcctatac cata
//
LOCUS       DAOPID010000054         7632 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_12260_length_7632_cov_4.324799, whole genome shotgun sequence.
ACCESSION   DAOPID010000054 DAOPID010000000
VERSION     DAOPID010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 7632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7632
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12260_length_7632_cov_4.324799"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="PK641_03000"
     CDS             <1..643
                     /locus_tag="PK641_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55352.1"
                     /translation="TACSNTKMLSSWRAESVPNNTMDKVLIFSLLGIKGNNDVQDDFE
                     DAIVSDLHRNGINAYSAYDIYGSAGLKSKSKETLAKEIHDGNYTGVMIITLLDKKKET
                     NYVPGSTYNVAVPIMPYGFGFYHYYNCYGYYYDQVTTPGYYTTDTEYILEARIFNTQD
                     AKDAIYIGKTSTMNPSDAESMAKDFSNTITHDLFNQGIIQNKKYKSDTSRFDY"
     gene            1000..2319
                     /locus_tag="PK641_03005"
     CDS             1000..2319
                     /locus_tag="PK641_03005"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPG55353.1"
                     /translation="MKVMKFGGTSVGSAQRMKEVTKLISDKEQKIVVLSAMSGTTNTL
                     VEIADYLYKKNSVSANEVINTLEAKYDSVINELYTSESYKEQAKSSIKKNFDLLRALA
                     KNNFFTVFEEKTVLAQGELMSTAMVNLYLNEIGQKSVLIPALDYMRIDKNNEPDAAYI
                     REHLVKELAKHPQDTDIYITQGYICRNAYNEIDNLQRGGSDYTASLIGAAILASEIQI
                     WTDIDGMHNNDPRFVENTKPVAKLHFEEAAELAYFGAKILHPTCILPAKLENIPVRLL
                     NTMEPNAPGTLISTDITKHKIEAVAAKDNITAIKIKSGRMLLAYGFLRKVFDCFEIHK
                     TSIDMVTTSEVGVSVTIDNTKHLEEIVNDLKKFGTVTVEKDMVIICVVGDMNSENVGF
                     QAKVVNALKDIPVRMISYGGSNYNVSFLVQASDKKRALNLLSEHLFN"
     gene            2329..3486
                     /gene="lysA"
                     /locus_tag="PK641_03010"
     CDS             2329..3486
                     /gene="lysA"
                     /locus_tag="PK641_03010"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPG55354.1"
                     /translation="MIKGKFPIDKFKGIKTPFYYYDLGLLRRTLDILKAETDKYGFTE
                     HYAVKANANPTILKIISSYGFGADCVSGGEIKAALEAGFPANRIVFAGVGKADWEIEL
                     GLDNDIFCFNIESLAELNVINEFAAAKHKVATIEIRVNPDIDAHTHSKITTGLQENKF
                     GINLEQLDDIIETANKLKNINFIGLHFHIGSQITDMSVFKDLCLRVNEIQYNLLQKGI
                     HLQNLNFGGGLGVDYHHPNHYSIPDFTNYFAVFNKLVERRENQDIHFELGRAVIAQCG
                     SLISKVLYIKEGQKKKFAILDAGMTDLIRPAMYDAYHRPENITSDLEIDTYDIVGPIC
                     ESSDVFGKDIELNKTKRGDLIAFRTAGAYGEVMASQYNLRAIAKSYFSDTL"
     gene            3489..4676
                     /locus_tag="PK641_03015"
     CDS             3489..4676
                     /locus_tag="PK641_03015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPG55355.1"
                     /translation="MAYNYLNYILDYFYGLPIWLGIVIGCAFCFFIIQMVYYWGFFTN
                     IFRFKRKCRKNKVSFLESFPPVSVIICAKNEEVNLNDKLPKVLEQDYPDYEVIVVNDS
                     SSDETEEVLAIMKTRYERLHTTFVPENAKFIDSKKFALTLGIKAAKNEILIFTDADCE
                     PKTEKWLKNIVRNFTEDTQIVLGYGGYKRKKSLLNYIIVFDTLFIAIQYINYSLSGKT
                     YMGVGRNMAYRKSMFMQSKGFTAHLNLQSGDDDLFINETATKTNTRVEISPDSITISE
                     PKDTFRMWKRQKERHLSTSGHYRFGSKALIGVEVFSRGLFYASLIALLISFDLVAAYI
                     ATGMFLIRYITQYIIINLTAKQLGERKYYIGILILDILLPLINLNIQIKSLFRGKEKY
                     KWK"
     gene            4760..5593
                     /gene="bamD"
                     /locus_tag="PK641_03020"
     CDS             4760..5593
                     /gene="bamD"
                     /locus_tag="PK641_03020"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HPG55356.1"
                     /translation="MKKVCLYLLFLLLCTSPVYGSAYSKLLKSPDTELKYNAALNYYE
                     KGDYTKTITLLEDISRTYKGTETAENILYVLSSSYFKKKDYISASHYYNTYVNTYLRG
                     SHYTECLFMLAYCYYKQSPHPELDQTETLKAIERFQIYINQFPNDSRIPEAKKMLGEM
                     YNKLADRELYNARLYYNLGNYKGNNYRAAIVTAHNAMNDYPDSPYKEEFALIIVRSKY
                     KEAVNSVQKKVKDRSEDAWDECYYFLQEYPESKHKKEVEKIASSLNKIIKPTEVETSL
                     K"
     gene            5599..5925
                     /locus_tag="PK641_03025"
     CDS             5599..5925
                     /locus_tag="PK641_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HPG55357.1"
                     /translation="MDYKKSNASLSTITRDVNNLCENTANVYETVAIISKRANQISVE
                     IKEELKRKLEEFSSNNVDNLEEVFENREQIEISKYYERLPKPTLLATQEYIEDKTYFR
                     NPAKIK"
     gene            5929..7125
                     /gene="coaBC"
                     /locus_tag="PK641_03030"
     CDS             5929..7125
                     /gene="coaBC"
                     /locus_tag="PK641_03030"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005843447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HPG55358.1"
                     /translation="MQGKHIVLGITGSIAAYKAAYLVRGFIKKGAEVKIIMTENAKQF
                     ITPLTMATLSQNPIAVEFFDPTNGEWHSHVKLGLWADVFLVAPATANTLGKMANGIAD
                     NLLTTTFLSAKCPTVVVPAMDVDMYSHPAMRNNLNALRNMGVGILEPETGELASGLLG
                     KGRMPEPETILEYMESFFNKEQSMSKKKVLITAGPTYEKIDPVRFIGNYSSGKMGYAL
                     ADECAKQGAEVCLISGPVSIKPTQNNIKVISVESANEMYTAAIEQFQQADIAILCAAV
                     ADFTPENKADNKIKRGKDGLTLNLIPTQDIAAELGKSKKSNQILVGFALETNNETAHA
                     IDKLKRKNLDFIVLNSLNDEKSCFGHDTNKVCIIDKKGEINNFPLKNKQEVAKDIINK
                     ITTWKD"
     gene            7113..>7632
                     /locus_tag="PK641_03035"
     CDS             7113..>7632
                     /locus_tag="PK641_03035"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HPG55359.1"
                     /translation="MERLRTLFLILASILIVGIAQAQELNCKVTINSDQIQGTNKSVF
                     EALQKAVTEFMNERQWTDYEYQVSERIECSLYIVVKTYSSNTFSAELQIQANRPIYNS
                     SYKSPIFSFTDKNFNFNYTEGDPLNFDATSFGNNLAEVLAYYAYIIIGTDCDSFSKLG
                     GTPFYRKAEQIVN"
BASE COUNT         2807 a         1255 c         1305 g         2265 t
ORIGIN      
        1 tacagcttgc agtaatacca aaatgttatc atcttggaga gcagaatcag taccaaataa
       61 cactatggac aaagtactaa tattttcgct actcggcatt aaagggaaca atgatgtaca
      121 ggacgatttt gaggatgcaa ttgtttcaga tttacaccgc aatggtatta atgcctattc
      181 agcatacgac atttatggtt cagcaggttt aaaaagcaaa agcaaagaaa ctcttgcaaa
      241 agaaattcat gatggtaact atacaggagt aatgataata acattgttag ataaaaagaa
      301 agaaactaat tatgttccgg gcagtacata caatgtagcc gttcctataa tgccttacgg
      361 attcggattc tatcactatt acaattgcta tggatattat tatgaccaag taactactcc
      421 aggatattac actacagaca cggaatatat ccttgaggct agaatattta atactcaaga
      481 tgcaaaagac gccatttaca taggtaaaac atcaacaatg aatccttctg atgcggaatc
      541 tatggcgaaa gatttttcta ataccataac gcacgactta ttcaaccaag gtatcattca
      601 aaataagaaa tataaatcag atacatcaag attcgattat taatagtgaa taaatgtgtt
      661 tttatctgta aattatttta gctaaaaacg ggttaaaatg gcaattatgt ataaataaat
      721 gttaaaataa ttacattcga ctgctttttt ttactgaata atttgcagca tatatcaaga
      781 aactttacct ttgcgtcaat attagttttc ttcgtaagaa aaagattaag aattaagatg
      841 gggcaagtgg tgatcaggcg tgatcgccac ttgtttttta tatatacccc aaacaaaaaa
      901 aataatctaa taaacgagca aatcatttta cttttctggc tataatatcc attatattta
      961 ctaattttgc atcgttaaaa ctaaactaaa taacagccca tgaaagtcat gaaattcggt
     1021 ggaacatccg taggttctgc ccaaagaatg aaagaagtta caaaactaat ttccgacaaa
     1081 gagcaaaaaa tagtagttct atcagcaatg tcaggaacaa ccaacacact tgttgaaatt
     1141 gcagattatt tatacaaaaa gaattctgta agtgctaatg aggtaattaa tactttggaa
     1201 gcaaaatatg attccgtaat caacgaatta tacacatctg aatcatacaa agaacaagca
     1261 aaatccagca taaagaaaaa ttttgattta cttcgtgcac ttgctaaaaa taatttcttc
     1321 acagtatttg aagaaaaaac agttttggca caaggagaat taatgtctac agctatggtc
     1381 aatctttatt taaacgagat tggacaaaaa tcagtattaa ttcctgcatt agactacatg
     1441 cgcattgaca agaataatga acctgatgct gcatacataa gagaacattt ggtaaaagag
     1501 cttgctaaac atcctcaaga taccgatata tatattacac aaggttatat ttgccgtaac
     1561 gcttataacg aaatcgacaa cttacaacgc ggaggtagtg attatacagc ttcattgatt
     1621 ggtgcagcaa ttttagcttc tgaaattcag atatggacag atattgacgg aatgcataat
     1681 aacgaccctc gttttgtaga aaacacaaaa cctgttgcaa aattgcattt tgaagaggcc
     1741 gcagaacttg catattttgg tgcaaaaatc ttgcatccta catgtatttt acctgcaaaa
     1801 cttgaaaata tacccgtacg ccttctaaat acaatggaac ctaacgcacc gggtacgctt
     1861 atttcaactg atataacaaa acacaaaata gaagctgttg cagcaaaaga taatatcact
     1921 gcaattaaaa tcaaatcagg acgcatgctt ttggcatacg gattcttacg taaagttttt
     1981 gattgctttg aaattcacaa aacttctatc gatatggtta ccacatcaga ggttggtgtt
     2041 tctgttacaa ttgacaacac aaaacattta gaagagatcg tcaacgatct taaaaaattc
     2101 ggtactgtga ctgtcgagaa agatatggtt attatctgcg ttgtaggtga tatgaactca
     2161 gaaaacgtag gttttcaagc taaagttgta aatgcactta aagatatacc tgtacgaatg
     2221 atatcttacg gtggaagtaa ttacaatgta tctttcttgg tacaagcaag tgacaagaaa
     2281 cgtgcgttaa acttacttag tgaacattta ttcaattaat aaagtaaaat gattaaaggt
     2341 aaattcccta tagataagtt taaaggtatt aaaactccat tttactatta cgatttaggt
     2401 ttacttcgtc gcactctaga tattttaaag gcagaaacag ataagtacgg atttacagag
     2461 cactatgctg ttaaggcaaa tgcgaaccct actatactta agattatttc gtcttacgga
     2521 tttggtgcag attgcgttag cggtggagaa ataaaagcag ctttagaagc cggattccct
     2581 gctaaccgca tcgtatttgc aggagttggg aaagcagatt gggaaataga acttggattg
     2641 gataacgata ttttttgctt taatattgaa tcattagcag agttaaatgt tataaatgaa
     2701 tttgcagctg caaagcacaa agtggcaact attgaaattc gtgttaatcc tgacattgat
     2761 gcccacacac attcaaaaat cacaacagga ttacaagaaa ataagtttgg tattaatctt
     2821 gaacaattag atgatataat tgaaactgca aataaactta aaaacattaa ctttattgga
     2881 ttacatttcc acatcggttc tcaaattacc gatatgagtg tttttaaaga tttgtgtttg
     2941 cgagtaaatg agatacaata caatttacta caaaaaggca tccatttgca aaatcttaat
     3001 ttcggaggtg gattaggtgt tgattatcat catccaaacc attatagtat tccggatttt
     3061 acaaattact tcgcagtatt caataaatta gttgaaagac gcgaaaatca agatattcac
     3121 tttgaattag gcagagctgt tattgcacag tgcggttcac tgatttcaaa agtcttatac
     3181 ataaaagaag gccaaaagaa aaaatttgcg attcttgatg ctggaatgac tgacctgata
     3241 agacctgcga tgtatgatgc atatcataga cctgagaata ttacttccga tttagaaata
     3301 gatacttacg atatagttgg tcctatctgc gaatcatcag acgtatttgg taaagacatt
     3361 gagttgaaca aaacaaaacg cggagattta attgcattta gaacagccgg agcatatggt
     3421 gaagttatgg catctcaata caatttacgc gcaatagcaa aaagttattt ctctgatact
     3481 ttataaatat ggcatataac taccttaact acattttaga ttatttttac ggacttccaa
     3541 tatggctagg tattgttata ggatgtgcgt tttgcttctt tattatacaa atggtttatt
     3601 attggggatt tttcaccaat atttttagat tcaaaaggaa atgtagaaaa aataaagtaa
     3661 gttttttaga atcttttcca cctgtatcgg taataatttg tgccaaaaac gaagaagtca
     3721 accttaatga taaacttcca aaagttctgg agcaggatta tccggattac gaagttattg
     3781 tagttaatga tagctcgtct gacgaaactg aagaggtatt agccataatg aaaacaagat
     3841 atgagcgact acacactact ttcgttcccg aaaatgcaaa attcattgat tcaaagaaat
     3901 ttgctttaac cttaggaata aaagctgcaa agaacgaaat attaatcttt actgatgcag
     3961 actgcgaacc taaaactgaa aaatggttaa aaaatatcgt taggaacttt actgaggaca
     4021 cacaaattgt gttgggttat ggcggttata aacgtaaaaa atcattattg aattatataa
     4081 ttgtatttga tacattattt attgcaattc aatacataaa ttattcatta tcaggcaaaa
     4141 catatatggg cgttggtaga aatatggcat acagaaagag tatgttcatg caatccaaag
     4201 gatttactgc acaccttaac ctacaatcag gagatgatga tttatttata aatgaaactg
     4261 ccactaaaac taatactcgt gttgaaatat ctccggatag tataactatt tcagagccta
     4321 aagatacttt tagaatgtgg aaacgtcaaa aagaacgtca cctatcaaca tcaggccact
     4381 atcgttttgg tagcaaagca ttgattggag tagaagtttt ttctcgtgga ttgttttacg
     4441 catcactcat tgccctacta atatcttttg atttagtagc agcttatatc gcaactggta
     4501 tgtttctaat aagatatatt acacaatata ttattataaa tcttactgcc aagcaattag
     4561 gcgagcgtaa atactacata ggcatactca tattagatat tttactgcca ttaataaatc
     4621 ttaatattca aattaaaagt ttgtttaggg gtaaagagaa atataaatgg aaataaagaa
     4681 atactaaatt ccgtcttttt acaaaacttt catactacga ttttttgtct ttcaagtaat
     4741 tgtcgtatct ttgcaaccca tgaaaaaggt atgcttatat ttattattcc ttttgttatg
     4801 tacttctcct gtttacggaa gtgcatactc taagttgctg aaaagccctg ataccgaatt
     4861 aaagtataac gcggctttaa attactatga aaaaggagat tacactaaaa cgattactct
     4921 actcgaagat atttcacgca catacaaagg aacagaaaca gccgaaaata ttttatatgt
     4981 tttatcttca agttatttta agaaaaaaga ctatatatct gcatcacact attacaacac
     5041 ctatgtaaat acttatttac gtggttctca ttatacggaa tgtctgttta tgctggcata
     5101 ttgctattac aaacaatctc cacaccctga attagaccaa acagaaactt taaaggctat
     5161 tgaaagattt caaatatata tcaatcaatt tcccaacgat tcgcgcatac ctgaagctaa
     5221 aaaaatgcta ggcgaaatgt acaacaaatt ggctgataga gagttatata atgcacgatt
     5281 atattataat ttaggcaatt acaagggaaa caactataga gcagctattg ttacggctca
     5341 caatgctatg aacgattatc ctgattctcc ttacaaagaa gaatttgctt taattattgt
     5401 acgttcaaaa tataaggaag ctgtaaatag tgtacaaaaa aaggtaaaag acagaagtga
     5461 ggatgcttgg gacgagtgct attatttttt acaagaatac cctgaaagca agcacaaaaa
     5521 agaggtagaa aaaattgcct caagcctcaa caaaataatc aaaccaacag aagtagaaac
     5581 tagtttaaaa taaaagccat ggattacaaa aaatcaaacg catctctttc aactatcaca
     5641 cgtgatgtaa ataatttgtg cgaaaacaca gctaatgttt atgaaaccgt tgctattatt
     5701 tcaaaacgtg ctaatcaaat tagcgttgaa ataaaggaag aattaaaacg caaactagaa
     5761 gagttttcaa gcaacaatgt agacaacttg gaagaagtat ttgaaaaccg cgagcaaata
     5821 gagatttcaa aatactacga acgtttacca aaaccaacat tgcttgctac tcaagaatac
     5881 attgaggaca aaacttattt ccgcaaccct gctaaaatta aataatagtt gcaaggtaaa
     5941 cacatcgttt taggtattac aggtagtatt gccgcatata aggcagcata cctagtacgt
     6001 ggttttataa aaaaaggcgc ggaggtcaag atcattatga ccgaaaatgc taagcaattt
     6061 ataactccac taactatggc taccctatct caaaacccga ttgcagtaga attctttgat
     6121 cctacaaacg gagaatggca tagccacgta aaattaggtc tgtgggctga tgtattccta
     6181 gttgctccgg caactgcaaa caccttaggt aagatggcaa atggcattgc agacaactta
     6241 ctcaccacaa cgtttttgtc agctaaatgt cctaccgtag tcgttccggc aatggatgta
     6301 gatatgtatt cacaccctgc aatgcgtaat aacttaaatg ctttacgtaa tatgggagtc
     6361 ggcatcctag aacctgaaac cggagaatta gcaagtggtt tactaggcaa aggaagaatg
     6421 cccgaacctg aaacaatttt agagtacatg gaatcttttt tcaacaaaga acaatccatg
     6481 tctaaaaaaa aagttctgat aacagccggt ccaacctacg aaaaaattga tccggtacgc
     6541 tttattggca actattcaag cggcaaaatg ggctatgcac ttgccgatga atgtgcaaaa
     6601 caaggagcag aagtttgttt aatatctgga cctgttagca tcaaacccac acaaaataat
     6661 atcaaggtta tatctgttga atctgcaaac gaaatgtata cagctgccat tgagcagttt
     6721 caacaagcag atattgcgat tttgtgtgca gcagttgctg atttcacgcc agagaacaaa
     6781 gctgacaaca aaataaagag gggtaaggat ggtttaactt taaatttaat accaacacaa
     6841 gatattgcag ctgaacttgg gaaatcaaag aaatcaaatc aaatattggt tggttttgcc
     6901 ttagaaacca ataacgaaac tgcccatgca attgacaagt taaagcgaaa gaatttggat
     6961 tttattgttt taaactcact gaatgatgaa aaatcatgct ttggacatga tacgaataag
     7021 gtatgtatta ttgacaaaaa aggagaaata aacaatttcc cattaaagaa taaacaggaa
     7081 gtagctaaag atattatcaa taaaatcacg acatggaaag actaagaaca ttatttttga
     7141 ttcttgcaag tatcttgatt gtcggtatag cacaggctca agagttaaat tgtaaggtaa
     7201 caataaattc tgaccagatt caaggcacaa acaaatctgt atttgaggct ttacaaaagg
     7261 ctgttactga gttcatgaac gaaaggcaat ggacagatta tgaatatcaa gtatctgaac
     7321 gcattgaatg ctctctttat atagtagtta aaacatattc aagcaacaca ttctctgccg
     7381 aattgcaaat acaagcaaat cgtcctattt ataatagctc atacaaatct cctatattca
     7441 gtttcactga taaaaacttt aattttaact acaccgaagg cgaccctctt aattttgatg
     7501 ctacaagttt tgggaataac ttagctgaag tattggcata ctatgcatat ataattatag
     7561 gaactgattg cgactccttt tcaaaactag gtggaactcc attctacaga aaagcagaac
     7621 aaattgtaaa tc
//
LOCUS       DAOPID010000055         7546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_12477_length_7546_cov_4.084902, whole genome shotgun sequence.
ACCESSION   DAOPID010000055 DAOPID010000000
VERSION     DAOPID010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 7546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7546
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12477_length_7546_cov_4.084902"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2711
                     /locus_tag="PK641_03040"
     CDS             <1..2711
                     /locus_tag="PK641_03040"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HPG55360.1"
                     /translation="LAPFKVNATASQTKSIVIVDTKINADKVCIDDSLLAIKNQKFDL
                     ELRANCDPKFKHFNINDLLVNIDKNHLALAGYLGIEDADSSYYTDLKLKFASPYLKKL
                     LALTPKPFKKYLKGFNFDGALGIEASSKGYMKGKHYPVIAAHVKLDKLHGKFDGFKQS
                     VDDLGFDIKANYNDRRKDSSFVDIKELHFKSGDSNLKTKGRACYKKGKEYVDISLLAH
                     LNLHTINNLYKIEENSEMKGNIDADVNTYFLLEDLKNKNLYNIFSKSSITGDGVEVVL
                     PQSGIDLYVDSLRARINTNTGVASRRRGTIDTSLVNTRISFRKMDLKYNKGISASANR
                     FSFSFLADDIEDGKPPRLRTSVSLRGLQASVFDTIQLTAKRARLSVNVQKDLNHGFIP
                     NLSIKLSLDSVLGSTPKLGVLLDSTRIKLSATPRFRKRKKLADGTRVDIPDFEQKVID
                     MKALSALFDTISKTKEPAELYMKKFSNNGDIYIKRFRIKSPDFPLRTSINRVDVDFTD
                     DTLHLDNLRLRMGRSAVTLTGQLENMRRYLLRGKTLKADLSLKSKRVDVNQLLSAFYA
                     SNQVQETKQNTDEKAKSVNVNLNALNESDDLDDNSTIDSTATAGLIVLPNNIDLKFKA
                     KIDTVKFGKMRLEDFKGKITMRNQSLRIKELFTNTQIGKANMNVMYNCSDPKTATAAA
                     SVVMDSVQIGDIVEYIPELDTLMPMLRSFDGNIYCEASGSTKLDSTMSIFLPTVNAAL
                     LLKGNDLVLMDGETFSEIAKMLMFNKKTRNLIDSVSVELLVRNNEIQIFPFMISLDKY
                     RLGVGGTQNLDMSFNYHIALLKSPIPFMMGVDVSGPNFDKIKYKLVSPKFKDSNVQIG
                     KGGTLINDGSTNIRKVFHKTMLNAILQEQELKEPTRRQQTTTEGNQ"
     gene            complement(2715..3896)
                     /locus_tag="PK641_03045"
     CDS             complement(2715..3896)
                     /locus_tag="PK641_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HPG55361.1"
                     /translation="MDQIFTDRMHDELQIRWDNLQTRMKAENVNACLITSNVNIYYML
                     GRIISGYVYILQEGEPWIFVRKPVELEGDKVRYIRKAEQIPDILAAEGIALPNILLME
                     GDELPYSEWMRYQDALRPVAVVNGSSLIRELRSVKTEYELEQLKFSARLQSQALREVP
                     LLYRSGMTDHQLTVEVERIFRLKGCLGAFRIYGRSMEAFMGSTLVGDNAAYPSPFDFA
                     LGGEGMHPSLPIGHKGTLLEPGMSVMIDINGNFSGYISDQSRTFSIGKIDEKADYAHR
                     VSLDIQNELAEMGKPGVSCGELYDRAMEIVVRHNLQDCFMGTKQQAKFVGHGIGLVIN
                     ELPVICGNNKMELKEGNAIALEPKFVINGVGAVGTENSFIVKNNGMEKITFAPEEIIE
                     L"
     gene            4041..5969
                     /locus_tag="PK641_03050"
     CDS             4041..5969
                     /locus_tag="PK641_03050"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPG55362.1"
                     /translation="MNQYLTIITGLILCTNFIQAQDNKLSGTAIGSSPSVDYNTNTPS
                     TTVNTPNYVFDGNLNTFYASFDRSNTWVGMDFGTACRITKIAYSPRISQPGRTLLAIF
                     EGANNADFSDAIPLFIIPTAATEGVMTYTDINCTRGFRYVRYISPNDARCNIAELAFY
                     GYQAQGDDSVLPQLTSIPTISIHTENCVDVTSKEEYLIGTATLVYNNGSAIWQDSLQI
                     RGRGNASWKFPKKPYRIKLNNKANLAGLPAKDKNWTLINNFGDKTLMRNLLANDISRR
                     LNMPYTPSGIPVDLVLNGEYKGCYQLCDQIEVGKNRVDIDKMAITDVDGENLKGGYLV
                     EIDAYANEGTSWFNSSRGNPVTIKYPKDDEIVWQQRNYIETYFNEMENILYSANYKDL
                     NIGYRSRLDLDTFLKHFIVGELSGNTDTYWSVYMYKYRLDDRFYTGPIWDFDLAFEND
                     SRTYPINNLGDYIYATNGSCAGNMRSFVNRILSDTKALDRLSDIWSIARHNDYLSADS
                     LIELIDRYAEEMDASQRLNFIRWPILNGTYEGELNNVKYYLSNRFTWLDNKIGLKEVT
                     EIDEEQTNQQECIVFTEKNIIIVKNASEKRIIIYSVDGKILYSNESVDAETINVPTPN
                     NGLYIINIDGISQKIFVK"
     gene            complement(6185..>7546)
                     /gene="mutS"
                     /locus_tag="PK641_03055"
     CDS             complement(6185..>7546)
                     /gene="mutS"
                     /locus_tag="PK641_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPG55363.1"
                     /translation="VIRQGINADLDEYRRLAYSGKDYLLQIQQRESDATGIPSLKISY
                     NNVFGYYIEVRNAHKDKVPQEWIRKQTLVNAERYITQELKEYEEKILGAEEKVLAIEN
                     QLFSALVTDLAGSVAVLQKDAVQIARLDVLCSFALVAAEGKYIRPLISDDDVLDIRQG
                     RHPVIERQMPIGEEYIANDVYLDSNKQQIIIITGPNMAGKSALLRQTALITIMAQIGS
                     FVPAESAQIGVVDKIFTRVGASDNISMGESTFMVEMNEAASILNNLSDRSLILFDELG
                     RGTSTYDGISIAWSIVEYIHEHPTAKARTLFATHYHELNEMEKDFKRIKNYNVSVKEI
                     DGKVIFLRKLVPGGSEHSFGIHVAKLAGMPKSIVKRAGDILLDLENKNQDNRKVSKKS
                     ISKPISEIAEHREGYQLSIFKLDDPVLSQVRDEILNVDVNNLTPLEALNKLSDIKKIL
                     KGK"
BASE COUNT         2572 a         1354 c         1276 g         2344 t
ORIGIN      
        1 aattagcacc attcaaagta aacgcaactg caagtcaaac aaaaagtatt gtaattgtag
       61 atacaaaaat aaatgctgac aaggtatgta ttgatgattc tttattggct ataaaaaatc
      121 aaaaatttga tcttgagctc agagctaatt gcgatcctaa atttaagcac ttcaatatca
      181 atgacttatt agtaaatata gataaaaatc acttagccct tgcaggttac ttgggaattg
      241 aggatgccga tagttcgtat tacactgact taaaattaaa atttgcgtct ccatatctaa
      301 aaaaactatt ggctttaact ccgaaaccat tcaagaaata tctgaaagga tttaattttg
      361 atggtgcact gggcattgaa gcaagttcca aaggttatat gaaaggtaag cactaccctg
      421 ttattgccgc acatgttaaa ttagacaagc tacatggtaa gtttgacgga tttaaacaaa
      481 gtgttgatga tttaggcttc gatataaaag ctaattataa cgataggcga aaagactcaa
      541 gctttgtcga tattaaagaa ctgcatttta aaagtggcga ttcaaactta aaaacgaaag
      601 gtcgtgcatg ttacaaaaaa ggcaaagagt atgttgatat aagcctactt gctcatctta
      661 atttacacac aatcaacaat ttatacaaga ttgaagaaaa ctctgaaatg aaaggaaata
      721 tcgatgcaga tgttaacact tatttccttt tggaggattt gaagaataaa aatctatata
      781 atatattttc aaaaagctcc attaccggag atggagttga agtggtttta ccacaaagcg
      841 gcattgactt atatgtagat tcattaagag ccagaataaa taccaacaca ggagtagcaa
      901 gcagaagaag aggaacgatt gatacctcat tagtaaacac acgtatttca ttcagaaaga
      961 tggatttgaa atacaacaaa gggatttctg caagtgctaa tcgttttagt tttagtttct
     1021 tagctgatga tattgaagat gggaaaccac ctcgtttaag aacatcagta tctctaagag
     1081 gtttgcaagc tagtgtattt gatactatac aattaactgc aaaacgtgca cgcctatcgg
     1141 taaatgtaca aaaagatttg aatcatggct ttataccaaa cttatctata aaattaagtt
     1201 tggattctgt gttgggatca actccgaaat taggtgtgtt acttgactca actcgtatta
     1261 aattatcggc aacaccacgt tttcgtaaac gtaaaaaact tgcagatggt accagagttg
     1321 atattcctga ttttgagcag aaagttattg atatgaaagc cttgtcagca ttgttcgata
     1381 ccatatcaaa aacaaaagag cctgctgaat tatacatgaa gaaattttct aataatggtg
     1441 atatatacat aaaacgcttt aggattaaaa gtcctgattt tcctttgaga acatcaataa
     1501 acagagttga tgttgatttt actgatgata cattgcatct ggataattta cgcctaagaa
     1561 tgggacgttc tgcagtaaca ttaacgggac aattggaaaa tatgagacgt tacttgcttc
     1621 gtggtaaaac gcttaaagcc gatttaagtc taaaatcaaa aagagttgac gtaaatcagc
     1681 tattgtcagc attttatgcc agcaatcaag tacaagaaac taaacaaaat actgatgaga
     1741 aggcaaaatc agtaaacgta aatctaaatg ctttgaatga aagcgacgac ttggatgata
     1801 attcaacgat agattcaact gctacagcag gcttaattgt acttccaaac aatattgacc
     1861 ttaaatttaa agcaaagatt gatactgtta agttcggaaa aatgcgatta gaagatttta
     1921 agggcaagat aacaatgcgc aatcaatcac ttcgcataaa agagctattt accaacacac
     1981 aaattggaaa agccaacatg aatgtgatgt ataattgcag tgacccaaaa acagcaacag
     2041 cagcagcatc agtagtaatg gatagcgtac aaatcggaga tattgttgag tatatacccg
     2101 aattagatac acttatgcct atgttacgtt cgtttgatgg taatatctat tgcgaagctt
     2161 ccggttcaac taaattggat tctactatga gcatattttt accaacagta aatgcggctt
     2221 tactattaaa aggaaatgat ctggtattaa tggatggaga aacattctct gagattgcta
     2281 agatgcttat gtttaacaag aaaacaagga atctgattga tagtgtatcc gttgaattac
     2341 tagtaagaaa caatgaaatt caaatattcc catttatgat aagtctcgac aaatacagac
     2401 ttggagttgg tggaacacag aatttggata tgagtttcaa ttatcatatc gcattgctaa
     2461 aatctccaat accttttatg atgggtgttg acgtatccgg tccaaacttt gataaaataa
     2521 agtataaact tgtttctcct aaatttaagg attctaatgt tcaaatagga aaaggaggta
     2581 cacttataaa tgacggcagc accaatattc gtaaagtttt ccacaaaact atgttgaatg
     2641 caatactaca agaacaggag ctaaaagaac cgactagaag gcaacaaaca actacagaag
     2701 gcaatcaata aatcctatag ttcaattatt tcttccggag caaatgtaat tttctccatt
     2761 ccattattct ttactatgaa ggaattttcg gtaccaacgg caccaactcc atttataaca
     2821 aacttaggtt ctaatgctat tgcgttacct tctttcagtt ccattttatt atttccgcaa
     2881 ataacaggta attcattaat taccaaaccg ataccatgac caacaaattt tgcttgctgc
     2941 ttagttccca taaagcaatc ttgcaaatta tgcctaacaa ctatctccat agccctatcg
     3001 taaagctcac cacaagatac tccgggtttc cccatttcgg ccaactcatt ttgtatatct
     3061 aaagaaactc gatgagcata gtctgctttt tcatcaatct ttcctataga aaatgttctg
     3121 gattgatctg aaatatatcc tgaaaaattg ccattaatat ctatcattac tgacattcct
     3181 ggctctaata aagtaccttt atgacctatg ggtagtgaag gatgcatacc ctcgcctccc
     3241 aaagcaaaat caaaaggaga cggatacgcc gcattatcgc ctactaatgt tgatcccata
     3301 aaggcctcca tcgatctacc atatattcta aatgctccca aacacccttt tagcctaaat
     3361 atacgttcaa cttcaactgt tagttgatga tctgtcatgc cacttctata taacaatggg
     3421 acttcgcgca aagcttgaga ttgtaagcga gcagaaaact ttaattgctc taattcgtat
     3481 tcggttttta cacttctcaa ttcacgtatt aaacttgagc cgtttactac agctaccgga
     3541 cgcagtgcat cttgataacg catccactca gaatatggta attcatcacc ctccattaat
     3601 aatatgtttg gcaaggcaat tccttcagct gctaatatat caggaatctg ttctgcttta
     3661 cgaatatatc ttaccttatc gccctccaat tcaacaggct tacgcacgaa aatccaaggt
     3721 tctccctcct gtaatatata tacatatcca cttattatgc gtccaagcat atagtaaata
     3781 tttacatttg aggtgattaa acaagcattt acattctctg ccttcattcg ggtttgtaga
     3841 ttatcccaac gaatttgcaa ttcgtcgtgc attcgatctg taaagatttg atccatattt
     3901 agaattttac acaaaagtac gaaaaaataa acaaaaaata tcatgttgta tttttatagt
     3961 acccactttt gattgcaatc attttattaa tattatcttt gtgtttgcta ggcgcgtttg
     4021 cttacttaaa taatcagccg atgaatcaat acttaacaat tattacaggt cttatacttt
     4081 gtaccaactt tatacaagca caagacaata aattatccgg tacagccatc ggctcttctc
     4141 cgtctgtgga ttacaacaca aatactccat caacaacagt aaatacacca aattacgttt
     4201 ttgatggaaa tctcaataca ttttacgcat ctttcgaccg atcaaacact tgggtaggta
     4261 tggactttgg aacagcctgc cgcataacca agatagcata ttccccacgt atatcacaac
     4321 cgggccgcac attattagct atttttgaag gggcaaataa tgccgatttt agtgatgcaa
     4381 taccattatt tattattcct acagctgcaa cagaaggagt aatgacatat actgatataa
     4441 attgtactcg cggttttaga tatgttagat atatttcgcc aaatgatgca cgttgcaata
     4501 ttgctgaact cgctttttat ggttatcaag ctcaaggaga cgattcagta ctacctcaac
     4561 ttacatcaat tccgacaata tcaatacaca cagaaaattg cgttgacgta acttctaaag
     4621 aagagtactt aattggcact gctacacttg tttataacaa cggaagtgca atatggcaag
     4681 atagcctgca aatacgtggt agaggcaatg caagttggaa atttcctaaa aaaccatacc
     4741 gcatcaaact taataataaa gcaaatttag caggtttgcc tgcgaaagat aaaaattgga
     4801 ctctaatcaa taatttcggt gacaaaactc tgatgcgaaa tttattggct aatgatataa
     4861 gtagacgttt aaatatgcct tacactccgt caggaatacc ggttgatctc gtattaaatg
     4921 gcgaatataa gggatgttat caattgtgtg atcaaataga agttggtaaa aaccgtgttg
     4981 atattgataa aatggcgatt actgatgttg atggtgaaaa tcttaaagga ggatatttgg
     5041 tagagattga tgcttacgcc aatgaaggca cttcatggtt taattcgtca agagggaatc
     5101 ctgttaccat aaaatatcct aaagacgatg agattgtttg gcaacaaagg aactatatcg
     5161 aaacgtattt taatgaaatg gagaatatat tatactccgc aaattataag gacttaaata
     5221 tcggatacag aagcagactc gatttggata cgtttttaaa acattttatc gttggcgaat
     5281 taagcggtaa tactgatacg tattggagtg tctacatgta taaatataga ttagatgacc
     5341 gtttttatac cggtccgata tgggatttcg acttagcatt tgagaatgac tcacgtactt
     5401 atccaataaa taatctgggt gattatatat atgcaacaaa tggctcttgc gccggtaata
     5461 tgcggagttt tgtaaataga attttatcag atacgaaagc tcttgatcgc ttatcagata
     5521 tatggagtat agccagacac aatgactatt tgtctgcaga ctcacttata gaattaatag
     5581 atagatatgc tgaagaaatg gatgcttcgc aaaggctgaa ttttataaga tggcctattc
     5641 ttaacggtac atatgaaggc gaactaaaca atgtgaagta ttacttaagt aatcgtttta
     5701 catggcttga taataaaata ggattgaaag aagttactga aattgatgaa gaacaaacaa
     5761 accaacaaga atgcatcgta tttactgaga aaaacattat aatagttaaa aacgcatctg
     5821 aaaaaagaat tataatttat tctgttgatg gaaaaatttt gtattctaat gagtcggtag
     5881 atgcagaaac catcaatgtg ccaacaccaa ataatggctt atatataatt aatattgatg
     5941 gcattagcca aaaaatattt gtaaaataat acacaggctt tattagcaaa acaaaacaaa
     6001 ctgaaagttg gcatttataa atttttagtt attgtaaatt tgcagaatta ataaattatt
     6061 gtacttttgc aaacgatctc tgagtgtaaa taacacaaaa tacatgtgta tccttatgat
     6121 gggtacgggt acacaataag ggcatctgct aaagcgggtg cccttatact tttatttgta
     6181 aggtttattt tcctttaaga atcttcttaa tatcgctcaa tttatttaaa gcttctagag
     6241 gagtgaggtt attgacatca acattaagaa tctcatcgcg tacctgcgaa agcacagggt
     6301 catcaagctt aaatatactc aactgatagc cttcacgatg ttcggcgatt tcggaaatag
     6361 gcttcgaaat agatttctta gatacctttc tgttatcttg atttttattc tctaaatcta
     6421 ataaaatatc accggcacgc ttaacaatac tcttaggcat tccggctaat tttgcaacat
     6481 gaataccaaa actatgctcg ctgccgccgg ggactaattt ccttaagaag attactttac
     6541 catctatctc tttaacagac acattgtaat tttttatacg cttaaaatct ttttccatct
     6601 catttagttc atggtaatga gtggcaaata aggttctagc ttttgctgta ggatgttcgt
     6661 gtatgtattc gacaatagac catgctattg agataccatc gtatgtactt gtgcctcgtc
     6721 ctaactcatc aaaaagaatt aaactacgat ccgataaatt gttaagaata cttgcagcct
     6781 cgttcatttc gaccataaag gttgactctc ccatcgaaat attatcgctt gcacctaccc
     6841 ttgtaaagat tttatcaact actccgatct gagccgattc ggcaggcaca aaacttccta
     6901 tttgagccat aatagtaata agtgcggttt gcctcaaaag agcagactta cctgccatat
     6961 tagggccggt aataattatt atctgctgtt tattactatc taaatacaca tcatttgcaa
     7021 tatattcttc tcctattggc atctgccttt caataacagg atgacgaccc tgacgtatgt
     7081 ctaaaacatc atcatccgaa attaatggac gtatatattt accttccgct gccactaatg
     7141 caaatgagca taaaacatca agacgcgcaa tttgaactgc atctttttgc aaaactgcca
     7201 ctgagcccgc caaatctgta actaatgcag aaaataactg attttcaatt gcaaggactt
     7261 tttcttcagc ccctaaaatc ttttcttcat actctttaag ctcttgagta atatagcgtt
     7321 ctgcattaac aagtgtctgc tttctgatcc attcttgtgg aactttgtct ttgtgagcat
     7381 tacgtacttc aatgtagtat ccaaaaacat tgttatagct aatcttaaga cttggtatac
     7441 cggttgcgtc actttctctt tgctgtatct gtaacaaata atccttgccc gaatatgcta
     7501 atcttctata ctcatctaaa tctgcgttaa ttccttgtct tataac
//
LOCUS       DAOPID010000056         7276 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_13082_length_7276_cov_4.593685, whole genome shotgun sequence.
ACCESSION   DAOPID010000056 DAOPID010000000
VERSION     DAOPID010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 7276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7276
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13082_length_7276_cov_4.593685"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..887)
                     /locus_tag="PK641_03060"
     CDS             complement(108..887)
                     /locus_tag="PK641_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPG55364.1"
                     /translation="MYALVTGASSGIGLKYATELASTYHCDLVLVSNQEKELQETAKD
                     LESRFGIKTVTVYKDLSTETAAEELHKFCAEKGIEIDILINNAGVFFFNELTKTEERR
                     VNLMLHLHIRTVTNMCRLFGNDMKERRRGYILNMSSMSAWMSMPGISTYNATKAYILN
                     FSRSLWYEMKPYGVGVTAICPGAVDTGLYGLSDYWRKVAVAINVSMRPEKLVKIALKK
                     MFKKKKQSMPGAINSLFVPFIKHLPDWAVFMVMKKISCFQK"
     gene            complement(1001..2359)
                     /locus_tag="PK641_03065"
     CDS             complement(1001..2359)
                     /locus_tag="PK641_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55365.1"
                     /translation="MKIVQTFWSGHKNHYNTYGWLSAKHHLLSWVLSSNQLRSFYDEV
                     ELVTDQYGYDLFIKQLRLPYSKVHVILDKLDYRDDLWAMAKIETYKYMKEPFLHVDGD
                     IYIWQQFPQKFANAEIITQNMEVTTESYYGNFWRKFSLKIKKLPELMVKYNTGAHNGA
                     FNTGLFGGNDIEFIQQYCNKAQEFVDDNKSDLYSLSLHEFNVFYEQVLLYEMTQAGGK
                     LVCSYYEESIKDNQYNINNIADFDTIPELNKYLHLLGGSKRIKSICDKLETYVLFFYP
                     EFYKRVEDILNINSNLRSSGYNYCRKHNNYLRDQYMSSLISKDFSCFNDTLNILGRDF
                     IAINSNYLTIKLCESNIKFYLIQMHNFSLKEGDELSKYGYIVIHGLGNDYTLIKKEKI
                     DTIILSELKVVRDNEEFMDRIMCHLNDRNSVKTNYFIKCVWSRIAFLASYKVIYISNT
                     IN"
     gene            complement(2325..3410)
                     /locus_tag="PK641_03070"
     CDS             complement(2325..3410)
                     /locus_tag="PK641_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55366.1"
                     /translation="MLIKDDIELTELANLLSQKNDRYISPEYLNENIIKKLIPYGVFV
                     GYDKFIIQNTKPSYLKLSFTIIPTFIVDKIAHWLIFLFNAKVFIILIILSCSILAYGT
                     YLNYNILLDMRIDKYWLILISFFISVTFHEFGHATAAKHFGANHNGIGGGFYLFHPVY
                     YADVTDIWRLSKYQRVIVDLSGIYFEFIFCSFVTLISILTNECTLFILAIIILSSTVY
                     NLNPFIRSDGYWAIIDITDETNIFNNPTQKLLHIYSCIRKHGLRYVVSIKNIIILLYA
                     FFSYVFQITFIYYAIIYNPKSILLFPINVYNYIVSIVNGDYLTVQTYLSLLMPLTFYI
                     LIFNYVKNKIKTYKNRHENSSNILVGS"
     gene            complement(3529..4527)
                     /locus_tag="PK641_03075"
     CDS             complement(3529..4527)
                     /locus_tag="PK641_03075"
                     /inference="COORDINATES: protein motif:HMM:NF024640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HPG55367.1"
                     /translation="MDGLKAVAIDYKRNYGELKHIMHLSKQDGFKNKNEIHVSDIFQR
                     AFIDNYLFDIASKYNSKTSEYFNNETQYFINSKKNDSIGVWSYFPTVKELSSDIDDLG
                     EIVRYFILVNRFDLIESICRKGILTAVQNQTKEGGIQTWILPKNKLSCLQKIQQNFND
                     SKWGNGPDIEVTANFVYSLFLYKPQLYDDLIAKALEYLVKNQNANGSWNCRWYSGQYY
                     GTFVCLRLLFAINDHIKYKDTLMSALSFLLNSQNNNGSFSTDGKESVISTAFALKCMK
                     YYPDKTKEAMSKAKKFLISTQSIDGCWEAETFIKPRLSETYKSKTLSTVIVLHSLL"
     gene            complement(4662..5402)
                     /locus_tag="PK641_03080"
     CDS             complement(4662..5402)
                     /locus_tag="PK641_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPG55368.1"
                     /translation="MDMQIIEKLRYFDKSPSFYQHYASLFSNYFPEISKTIIEELDNI
                     GYEYYHSILLLDSIVDESITKNLPQMVSLQEDAIKRLSQIFSYNCVFWKFWERRKYLF
                     FEAISQEKQLNYKNSNWTKYAKIADNKSAFGKIAIDCLFFLSCKKDETLYNALLESHK
                     NFSIGFQLYDDVCDFVHDLQNNQYNWGMQTMYIKDVQTSSDIEKNGKIFYLSGIACRT
                     LEKSIRYLKKAIYILKYFDCESDWIKQF"
     gene            complement(5404..6603)
                     /locus_tag="PK641_03085"
     CDS             complement(5404..6603)
                     /locus_tag="PK641_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55369.1"
                     /translation="MLKYYRITLCISLLLMGFAEYIFAGPINFKLIDKDNGEPIQFAC
                     ITANGFQTYSNLQGECIVDLPADSSITISHITYGKMIIPNNGNIPASFYWEKLSYDLG
                     ETSIIPPKKLNKILNSLRRKYLNYDDDAFGNKTFYYRQITDVDNMPTEYIESVIKAKT
                     SERLTNFNVISGRSAKLDSLNGQLVLNLMNAYSLGSVKLFSKSNAPTKNKVTPLFCNN
                     FLKIFDIKVDKIYFADTVDEMWVCEFYEKKYKSNNKRCYISGKISIKLKDLTIIASDI
                     HIRNINMISPKNTKTSNAELTIKTNYKSDNSPYPIVDTEQIDIKFDAQNLDLEKRHYN
                     VISYYTSIDTYTTSKGNKLGKIDNLFQILSSSTSDSLFLQQSLLIKRTEAEEKILNDF
                     KSKGLLH"
     gene            complement(6608..6763)
                     /locus_tag="PK641_03090"
     CDS             complement(6608..6763)
                     /locus_tag="PK641_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55370.1"
                     /translation="MNKTQNATIEKDCNEDDLLLTNFDIEELEMRYEMGWLSKPFGTG
                     SGTAVSF"
BASE COUNT         2357 a         1085 c         1034 g         2800 t
ORIGIN      
        1 tcctaatttt aaggatactc gaatagcaat gttgcgtgca tcaagcgttc atgaactctt
       61 ttcaattttt gatgatttca tcgaacgtta tggcaaatag cctgctttta tttctgaaag
      121 caagatattt tcttcataac cataaaaaca gcccaatcgg gtagatgttt tataaatggt
      181 acgaataaag agttgatagc tccgggcata gattgttttt tctttttaaa catctttttc
      241 agcgcaatct tgactaattt ttcagggcgc atgctaacat ttatagcgac tgcaacttta
      301 cgccaataat cactcaagcc atataacccg gtgtcaacag ctcccggaca tattgctgta
      361 acgcctacac cgtaaggttt catttcgtac cataatgagc gagaaaaatt cagtatataa
      421 gctttggttg cattgtatgt gcttattccg ggcatcgaca tccatgcgct catcgaactc
      481 atattaagaa tatatccgcg tctgcgttct ttcatgtcgt ttccaaaaag tctacacata
      541 tttgtaacgg tgcgtatatg tagatgaagc attaaattaa cgcgtctttc ttcagttttt
      601 gtaagttcat tgaaaaagaa tactcctgca ttgtttataa gaatatcaat ctcaatacct
      661 ttttcagcgc aaaatttatg aagttcttct gctgcagttt cggttgataa gtctttgtat
      721 acagttactg tttttatgcc aaagcgagat tctaagtctt tggctgtttc ttgaagttct
      781 ttttcttggt tgcttaccaa tacaagatcg caatggtaag ttgatgctaa ttcagtggcg
      841 tatttcaatc caattccgga acttgctccg gtaacaagtg cgtacatatc gtttttttta
      901 aattgcgatg caaaggtact aagaatttta ttaatctggt taagattata gtttgtataa
      961 aaaaataatc gaggcaaaca tcgccttaaa ttgacttata ttaatttata gtattagaaa
     1021 tatatattac tttatatgaa gctaaaaaag caattctaga ccaaacgcat ttgataaaat
     1081 aatttgtttt tacagaattg cgatcgttaa gatgacacat tattctatcc ataaattctt
     1141 cattatcgcg gacaactttc aattcggata atataatggt gtcgattttt tcttttttta
     1201 taagtgtata atcattgcca agtccatgta ttacaatata gccatatttg gaaagttcat
     1261 cgccttcttt tagtgaaaag ttatgcattt gtattaaata aaattttatg ttgctttcgc
     1321 ataacttgat tgtcaaataa ttactattta tagcaataaa atctctacct aaaatgttaa
     1381 gagtatcatt aaaacatgaa aagtctttac taattagcga tgacatatat tggtctctta
     1441 gataattatt gtgcttccta caataattat aaccagaaga tcttaaattg ctatttatat
     1501 ttaatatatc ttccacgcgt ttgtaaaatt caggataaaa aaataatacg tatgtttcaa
     1561 gcttatcaca tattgatttt atacgctttg atcctcctag taaatgaaga tatttgttta
     1621 attctgggat tgtatcaaaa tcggctatgt tattaatgtt gtattgattg tcttttattg
     1681 attcttcgta atatgaacag actaattttc ctcctgcttg agtcatctcg tataataaaa
     1741 cttgttcata aaacacattg aattcgtgaa gtgataatga atataaatcg gatttattgt
     1801 cgtcaacaaa ttcttgtgct ttattgcaat attgttgaat aaattcgata tcattgcctc
     1861 caaataatcc tgtattaaat gctccattat gtgcccctgt attgtatttc accattagtt
     1921 cgggaagttt tttgatttta agagagaatt ttctccaaaa atttccataa taactttcag
     1981 ttgtaacttc catgttttgt gtaattattt ctgcatttgc aaatttttgt gggaattgtt
     2041 gccaaatata aatatcacca tcaacatgta aaaatggctc tttcatatat ttatatgttt
     2101 caattttagc catagcccat aagtcgtcgc gataatcaag tttatcaaga attacatgta
     2161 ctttactata tggtaatcgc agttgcttta taaataaatc gtatccatat tgatcggtta
     2221 ccaattcaac ttcgtcgtaa aacgaacgta attgattaga gcttaaaacc caacttaata
     2281 aatgatgttt ggcagacaac catccatagg tattgtaatg gtttttatga cccgaccaaa
     2341 atgtttgaac tattttcatg tctattttta tatgttttaa ttttattttt tacgtaattg
     2401 aatattagta tgtaaaatgt tagtggcatt aatagagata aataagtttg cactgttaga
     2461 taatctccat taacaatact aacaatgtaa ttatatacat ttatagggaa caataatatt
     2521 gattttggat tatatattat tgcgtagtaa ataaaagtaa tttgaaaaac ataactgaaa
     2581 aatgcatata atagtattat gatgttttta attgacacta catatcgtaa tccatgtttt
     2641 cgaatacatg aataaatatg taatagtttt tgagtgggat tgttaaaaat gttagtttcg
     2701 tcagtgatat ctataattgc ccaataccca tcacttctaa taaaaggatt taaattatac
     2761 actgtagaac tcaatatgat tatggcaaga ataaataaag tacattcgtt tgtcaatatg
     2821 ctaattagtg taacaaaaga acaaaatatg aactcaaaat atataccaga caagtctaca
     2881 ataacacgtt gatatttact taatctccaa atgtctgtaa catcagcata atatacagga
     2941 tgaaataaat aaaaaccacc acctatacca ttatgattag ctccaaaatg tttggccgct
     3001 gtagcgtgtc caaattcgtg aaatgtaaca cttatgaaaa aacttatcaa tataagccaa
     3061 tatttatcaa tgcgcatatc caataggata ttatagttta agtatgtacc atatgcaaga
     3121 atagaacaag acaaaataat taatattata aaaacttttg cattgaaaag aaatatcaac
     3181 cagtgagcga ttttatctac aataaatgtt ggtataatag taaagctgag ttttagataa
     3241 cttggtttag tattttgaat tatgaattta tcatatccaa caaaaactcc atatggaatc
     3301 aattttttta ttatgttctc gtttaaatat tctggcgaga tgtatctgtc gtttttttgt
     3361 gaaaggagat ttgctaattc agtaagctct atatcatctt ttattagcat taacagatca
     3421 tacgtgttgc tgtcaatttt taaatagtgt cgatatcttg tatttattaa tagatatgat
     3481 ttactattat atttttttaa taaaatatca tctgaaatgc gcattgttct acaaaagaga
     3541 atgtaacaca attactgtac ttagcgtctt gcttttataa gtttcgctta accttggttt
     3601 gataaaagtc tctgcttccc aacatccatc tatagactga gtactaatca agaatttttt
     3661 tgcctttgac atagcttctt ttgttttatc aggataatat ttcatgcatt ttagagcaaa
     3721 tgctgttgaa atcacacttt cttttccatc tgtagaaaaa cttccattat tattttgtga
     3781 gtttaataag aaactcagtg ccgacattaa tgtatcctta tatttaatgt ggtcattaat
     3841 agcgaataaa agtctaagac acacaaaagt tccataatat tgtccagaat accatctgca
     3901 attccacgag ccatttgcat tttgattttt aactaaatat tccaatgctt tagcaattaa
     3961 atcatcgtat aattgtggtt tatataagaa taaactgtat acaaaattag cagtgacttc
     4021 aatatctgga ccattccccc attttgagtc attaaaattt tgttgtattt tttgaagaca
     4081 cgataattta tttttaggta aaatccacgt ttgaatacct ccttctttgg tttgattctg
     4141 tactgctgta agaattcctt ttctacatat actttcaatt aaatcaaatc gattaactaa
     4201 tataaaatag cgaacaattt cgcctaaatc atctatatca gaagataatt ctttgaccgt
     4261 cggaaagtat gaccatacgc caatgctatc attctttttt gaattaataa aatattgagt
     4321 ctcattatta aaatactcac tggtttttga attgtattta ctagcaatat cgaaaaggta
     4381 gttatcaata aatgctcttt gaaatatatc agaaacatga atttcgtttt tatttttaaa
     4441 tccatcttgt ttacttaaat gcattatgtg ctttagctct ccatagttgc gtttataatc
     4501 tattgcaact gctttaagcc catccataaa tactttatta cagtttaata gatttaaaaa
     4561 tggtttctcg tttacagatt ttagtaattt tgtttttatt cgtatactat taatgtatcc
     4621 gttaatattt gatatatagg tcctaatcgt agatattgtt tttaaaattg ttttatccaa
     4681 tcgctttcgc aatcaaaata ttttaaaata tatatagctt tttttaagta tcttattgac
     4741 ttttctagtg tgcgacatgc gatcccactt aaataaaata ttttgccgtt tttttctata
     4801 tcagatgatg tttgaacgtc tttgatgtac atagtctgca ttccccaatt atattggtta
     4861 ttttgtaagt catgcacaaa atcgcaaaca tcgtcatata actgaaatcc aattgaaaaa
     4921 ttcttatgtg attctaaaag cgcattgtat agcgtctcat cttttttaca cgaaagaaag
     4981 aataaacagt ctattgctat tttaccaaaa gcagatttgt tatcagcaat ttttgcgtat
     5041 ttagtccaat tagaattctt gtagtttaat tgcttttctt gtgatattgc ctcaaaaaat
     5101 aagtatttcc ttctttccca gaatttccaa aaaacgcaat tgtaagagaa tatttgggat
     5161 aatcttttta tagcgtcttc ttgtaaagat accatttgtg gtaaattctt agtaatagat
     5221 tcgtctacta tactatctaa caataatatg gaatgatagt attcatagcc tatattatct
     5281 aattcctcaa ttatagtttt gcttatttct ggaaaataat tagaaaacaa acttgcataa
     5341 tgctgataaa aagatgggga cttatcgaaa tatcttagtt tttcaattat ttgcatatcc
     5401 atattaatgc aataatcctt ttgatttaaa atcgttcaag attttttctt ctgcctctgt
     5461 tcttttaata agtaaggatt gttgtaagaa gagtgagtcc gatgtgctgc ttgataatat
     5521 ctgaaaaaga ttgtctattt ttcctaattt attcccctta cttgtagtgt aggtatcaat
     5581 tgatgtataa tatgaaatca cattataatg acgtttttct aagtctaaat tttgagcatc
     5641 aaactttata tcaatttgtt ctgtatcaac gataggatag ggcgaattat cacttttgta
     5701 atttgttttt attgttaatt cggcattact cgttttcgtg tttttaggag aaatcatatt
     5761 tatatttcta atatgaatat cagatgcaat aatagtcagg tcttttaatt taatactaat
     5821 cttgccactt atgtagcatc ttttgttgtt gctcttgtat tttttttcat aaaattcgca
     5881 tacccacatt tcatcaactg tatcagcaaa atatatctta tctactttaa tgtcgaaaat
     5941 ttttaaaaaa ttattacaaa ataaaggcgt caccttattc tttgtaggag cgtttgattt
     6001 agaaaatagt ttaactgatc cgagagagta tgcgttcatt agatttaata ccaattgtcc
     6061 gtttaggcta tctaattttg cgctacgccc tgatatgaca ttaaaatttg taagtctttc
     6121 agatgttttt gcttttatta cgctttcaat atattcggtc ggcatattat caacatctgt
     6181 tatttgtctg tagtaaaatg ttttattccc aaatgcatca tcatcgtagt ttaaatactt
     6241 gcgccttaaa gagtttaata tcttatttaa ttttttaggt ggtataattg aagtttctcc
     6301 taaatcataa cttaattttt cccaataaaa acttgccggt atattaccat tattaggaat
     6361 tatcattttc ccgtatgtta tgtgtgaaat agtaatagat gagtccgcag gcaaatcaac
     6421 tatacattct ccttgtaaat ttgaatatgt ttgaaatcca ttggctgtaa tacatgcgaa
     6481 ttgtattggt tcgccattgt ctttatctat aagtttaaaa ttaatgggcc ccgcaaatat
     6541 atattctgcg aaacccatta ataaaagact tatgcataat gtgatcctat aatatttaag
     6601 catattatta gaatgaaact gcagttccgc tacctgtgcc aaatggtttg ctgagccatc
     6661 ccatttcata acgcatttct aattcctcaa tgtcgaaatt tgtaagtaat aaatcgtctt
     6721 cgttgcaatc tttttcgatt gttgcatttt gtgttttgtt catagctgta atgttaaaaa
     6781 atcatattct tttttgcgag tacaaacgta gttgaatttt tatattgatg caaaagtttt
     6841 ttcgactttt taacaatgta ttgaacgttg taaatttttt caaaaggagc attggctata
     6901 gctaatttgt taaaagtttg taactttttt ctgtgatata ataagattat ttatctgtaa
     6961 agtagtaaat atatttgcac cttcgtttat tagtattata gaactagtgc tataaatgat
     7021 taataataag tttaatagat attatttttg ggaatttttc ctactgctag tcgttttaat
     7081 ttcggaaaaa acaagctatt atttatttat cttaggcgta gcaaatattg caaacaagca
     7141 ttttctctgt aatcgtttca aagcatttct gtgtagtaaa tgtataataa tttatcataa
     7201 aattgcggat taacgctttt tatagattaa atatttttta tatgtcaaat aaaacggtat
     7261 ctttgcattc gaatat
//
LOCUS       DAOPID010000057         7123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_13488_length_7123_cov_3.534663, whole genome shotgun sequence.
ACCESSION   DAOPID010000057 DAOPID010000000
VERSION     DAOPID010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 7123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7123
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13488_length_7123_cov_3.534663"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="PK641_03095"
     CDS             complement(<1..354)
                     /locus_tag="PK641_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPG55371.1"
                     /translation="MVKIVTGISRCGKSFLLMTLFHQHLCEQGVEENHIIELSLDNRK
                     NRRLRNPDTLLDYIDSKIQSDGQVHYIILDEVQLIDDFVEVLLSLMQDKRLDVYVSGS
                     NSKFLSKDVVTEFRGR"
     gene            858..1559
                     /locus_tag="PK641_03100"
     CDS             858..1559
                     /locus_tag="PK641_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG55372.1"
                     /translation="MIDIENINKSFDKKLIISDINLHIPKGEIICFLGPSGSGKTTLI
                     RLILGAISPDTGQIRINGIPMPNLEMLSRIGFMPQSDSIYDNLSAIDNLKFFGGLYKM
                     DKKTFNERIEKAIEMVGLTEDKFKLVSKFSGGMKKRLSLAIATLNDPDLLLLDEPTVG
                     IDPILRRSIWKQFLAWRDAGKTLLVCTHVMDEVNECNKAALINEGHLIAYDSVSNLLS
                     QAKNGHIEELFIKSK"
     gene            1570..2223
                     /locus_tag="PK641_03105"
     CDS             1570..2223
                     /locus_tag="PK641_03105"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG55373.1"
                     /translation="MKALIFRILKQLKNDPRTLAIMLVAPLLLMSLIYFLLSDNDYNA
                     RIGVYDIQESFIEVLSEEVEVKQLSDTSEINSFLKKDSVDAVLWADGDGIHTMVLENN
                     SKSAKAMQAVQRALSEMMSMGEFTTDSIYGSTDESYFSSMAYIFLTVVAFFFTFIVSG
                     MALVRERFTNTLERLLMSPIRRWQIVGGYIGGYGTAAVIQGVLNFCHEINCTDEILT"
     gene            2372..>2555
                     /locus_tag="PK641_03110"
     CDS             2372..>2555
                     /locus_tag="PK641_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55374.1"
                     /translation="MKKKSLFILMFLVAVATAAFAQPRAVGGRGRLGYGFEASYQHSI
                     GNNMVEVEVGLPGFFGV"
     assembly_gap    2556..2655
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2656..3009
                     /locus_tag="PK641_03115"
     CDS             <2656..3009
                     /locus_tag="PK641_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55375.1"
                     /translation="IGNNMVEVEVGLPGFFGVEAAATYDWIFPIRSWQEKGEWNWYAG
                     VGAGAGYFWPASGYVGAAGRIGVEYNFWFPLQLSFDWRPIVGPVFSFGGATAVKFNAG
                     GLYAGAIAFGIRYKF"
     gene            3326..3913
                     /gene="ruvA"
                     /locus_tag="PK641_03120"
     CDS             3326..3913
                     /gene="ruvA"
                     /locus_tag="PK641_03120"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HPG55376.1"
                     /translation="MFEFIKGDVVELTPTLAILENNGIAYSINITISTYTAIQGKKNV
                     CLYLYEAIREDAFILYGFAEKTERQMFLLLISVSGVGAGTARVILSSVSVPELQAAIA
                     GGNEALFKGVKGIGLKTAQRIIIDLKDKVLSIQTVPGAINISSALSEVQEEAVSALVM
                     LGFAAPAVRKVISKIAEADSSLSVEIIIKKALKMM"
     gene            3918..>7123
                     /gene="sprA"
                     /locus_tag="PK641_03125"
     CDS             3918..>7123
                     /gene="sprA"
                     /locus_tag="PK641_03125"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HPG55377.1"
                     /translation="MKKSVEKIFLILALLCGMVLIPVWAVEPVSEDAPNNTELAQDTS
                     KPRYQTNKIYPETQSDLEESAPIDLPAPENIKTKIDYDPTTGKFFFNPQLGEENVSTP
                     FYLNSDEYYDYSAKKSLESYYLSKNREEKKSKHQLSLTEMKFDIGQADKVFGPGGVQL
                     KLQGSAELDFGLDFKKLKDPSLNERSQNPAPTFDFDEKIQLSVDGSVGDKLKFGLNYN
                     TEATFDFDQSLLKLQYEGKEDDIIKKIEAGNVSMPLTGSLITGNTSLFGFKADLQFGK
                     LKINAVISQQESEGKTLNTSGAQTTDYEIRADAYDENRHYFLSHYFREHYDEWMSELP
                     NISSGISIGEIEVWITNKNTINYDNTSNVVGFADIAEPNRIYNSSFISTGNPTVPDNA
                     ANTLFADVVQSIGGLPNIYEVDTYLRSFTMQSTIDYVKLEGARKLESSEYTINKELGY
                     ISLKSALNNDEMLAVAYQYTYKGKVYNVGELSKGSEPTKPLSLKLLKSIDKSPFVPTW
                     DLMMKNVYALGAYQVQRDKFTFNIMYRNDSTGIDLRYISEGPIAKKPLIKVMNLDRLD
                     SHLQKTANGDGIFDYVEGYTIISQNGRVIFPVLEPFGSYLKKSLNNDPILVEKYVFQE
                     LYDSTLTVAQELAEKNKFHMVGEYRASSGSEIRLNAMNVPKGSVKVTAGGRELVENVD
                     YTVDYMMGVVSIMNQDLIDLGTPISVTMESQSMFNMKRKSLIGTHLDYAFNDHFNVGG
                     TIMYLSEKPLTEKVSYGDDPVANTIWGLNTSYRNQFQSVTDFLNKIPILNLKQPSSIA
                     FNAEFAQMVPGTAKGANGKVYVDDFESTKIGFDVRYASNWKLSSTPLRFEESANDNNI
                     EYGKNRALLSWYYIDNMFNRSTSSTPSYIRNDKEQLSNHFVREISEQEIFPNRELVYG
                     QSTYIQTLDLAYYPEERGPYNVFDQNFNSEGYLIDPKSKWGGIMRKLETTDFESNNIE
                     YLEFWMIDPFVYNDGTAKGGDLYFNFGDISEDVLKDGRKFYENGMPFDGSTDDVVETV
                     WGKVPSKQSLTYSFDSDAAARKLQDTGLNGLLTEEEFT"
BASE COUNT         2259 a         1107 c         1373 g         2284 t
ORIGIN      
        1 tctgccacga aactctgtta ccacatcttt cgatagaaac ttagagttag accccgatac
       61 atacacatcc aaacgcttat cttgcatcag acttagtaac acttcaacaa agtcatctat
      121 caactgcacc tcatcaagaa taatataatg cacttgtcca tccgattgaa ttttactgtc
      181 aatataatcc aataatgtat cggggttacg caagcgtctg ttcttgcgat tatccaacga
      241 taattcaatg atgtgatttt cttccacgcc ttgctcacaa agatgttgat gaaataaagt
      301 catcaaaagg aatgacttgc cgcagcggct aataccggta actattttaa ccataccatt
      361 accttttgcc gaaatcaact gctgaaggta tttatctcgt gatattaata tattagaggc
      421 gtttttatgt ggctatccac atattttata ctgcaaaatg tacaaaactt tatttaatta
      481 gcaataattt agtggtggga aaatttctcc catttgcaga aaaaaggtcg tataagttca
      541 gctgttcttt ttcttttcgc aagtacatcc tttttaacaa ggtattgcgt acttgctgcc
      601 gttcacgtag aacagcacct tgaatgtgct tctgctcatg tctaactttt ttggttgcag
      661 aactaataat caacgagtta aactttgaca agcaaaccta aacagagcga cgcaatcgct
      721 ttattcatat gttattcggt gtcattttcc cctttttatt cgtttattca ggagatttat
      781 tttatatttg tgtgatgaat aattgcgaat aaaaaactta tatttgcata agcaatccaa
      841 ttaatcctta ccatacaatg attgatattg agaatatcaa taaatcgttt gataaaaaac
      901 taataatcag cgatataaat ctacacattc caaaaggcga aataatctgt tttttggggc
      961 catccggttc aggaaaaacc acattaatac gtttaatatt aggtgcaata tctccagata
     1021 ccggccaaat acgcataaac ggaatcccaa tgcctaattt agagatgctg tcacgcatag
     1081 gttttatgcc acagagtgat tctatatacg ataacctatc cgctatcgac aatttgaagt
     1141 tttttggcgg tttgtacaaa atggacaaga aaacatttaa tgagcgaata gaaaaggcta
     1201 ttgaaatggt aggactaact gaagataaat tcaagctggt ttcaaagttt tcaggtggga
     1261 tgaaaaaacg cttatcattg gcaattgcta cactaaatga tcctgatttg ttattattgg
     1321 acgaacctac agtaggcata gacccaattt tgcgcagaag tatatggaag caattcttag
     1381 cttggcgtga tgcaggtaaa acattgttag tctgtactca tgtgatggat gaagtgaatg
     1441 agtgcaacaa agctgcctta ataaatgaag gacatttgat agcttacgat tcggtgtcaa
     1501 atttattaag tcaagcaaag aatggacata tcgaagaatt atttattaaa tctaaataaa
     1561 tcgtcaagaa tgaaagcatt aatattccgc atattaaaac aattaaagaa cgaccctcgt
     1621 acattggcga ttatgttagt ggctccactt ttattaatga gccttattta ttttttgttg
     1681 agcgataatg attataacgc acgtataggt gtttatgata ttcaggaatc atttatcgaa
     1741 gtcttatcag aagaagtgga ggtaaaacaa ttaagtgata catcagaaat taattcgttt
     1801 ctgaaaaaag attcggtaga tgctgtttta tgggctgatg gagatggcat acacactatg
     1861 gtacttgaaa ataatagcaa gtcggctaag gctatgcagg cagtgcaaag agctttaagt
     1921 gaaatgatgt ctatgggcga atttacgaca gatagtattt atggttcgac cgatgagagc
     1981 tatttttcgt caatggcata tatatttttg actgtagtag catttttttt tacgtttata
     2041 gttagtggta tggctttagt gcgcgagcgt tttacaaata ctcttgaacg tttgttaatg
     2101 agtccgatta ggcgttggca gattgttggt ggttatatcg gaggctatgg tactgctgcc
     2161 gttattcaag gtgttttaaa tttctgccat gaaattaatt gcactgacga gattttaaca
     2221 taatatacgt ctgattatat gtgttatgcg ctttatgaat gatttttcta aaaatatata
     2281 tgttaaaaaa ccgtaaaata tcaagatata acttatcttt gccggcgtat ctgagagata
     2341 taatttttct attaaactta aatattacaa aatgaaaaag aaaagtttat ttattttaat
     2401 gtttttagtt gcagttgcaa cagcagcatt tgctcaacct cgtgcagtcg gtggtcgtgg
     2461 tcgtctcggt tatggtttcg aggcatctta tcaacacagt attggcaaca acatggttga
     2521 agtcgaagta ggtttaccgg gattctttgg agttgnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnattgg caacaacatg gttgaagtcg aagtaggttt accgggattc
     2701 tttggagttg aagccgcagc tacttacgat tggatcttcc caatacgttc ttggcaagaa
     2761 aaaggagaat ggaattggta tgccggcgtt ggtgcaggag caggttattt ctggccagct
     2821 tctggatatg ttggtgcagc aggtcgtata ggtgttgaat acaacttctg gttcccactt
     2881 caattgtctt ttgattggcg tcctattgta ggacctgtat ttagctttgg aggcgctact
     2941 gctgtaaaat ttaatgcagg aggtttatat gccggtgcaa tagcatttgg tattagatat
     3001 aaattctaat tcactaaact attattatag tcttaatgag gctgcatcaa agatgcagcc
     3061 tcattttttt atatacccga aagataattt ctaaaacatt tagattgcat tacaattgta
     3121 cacactgacg agtatataaa atctgcatgc aaataataaa ttatgtataa ttaagcttct
     3181 gatttgttgt gttttgcagt ttgaagttaa aaaatactac gtatatacga tttccctttt
     3241 ttacacgtta gttattgtgt aagataaagg tttttcgtac ctttgacggc ttttattggt
     3301 tttcaccttt caatgctatt tatttatgtt tgagttcatt aaaggagatg ttgtggaatt
     3361 aactcccaca ctggcaatat tggagaataa tggtatagca tattctatta atattaccat
     3421 atctacatat actgcgatac aaggaaagaa aaatgtctgc ttgtacttgt atgaggctat
     3481 tcgcgaggat gctttcatat tgtatggctt tgcagaaaaa acagaaagac aaatgttttt
     3541 gttgcttatt tccgtttcgg gagttggtgc gggtacagcc cgtgtgatat tgtcttctgt
     3601 ttctgtgccg gagttacaag cggcaattgc cggcggcaat gaggcattat ttaaaggagt
     3661 taaaggcatt ggtttgaaaa cagcgcaaag aattataatt gatttaaaag ataaggttct
     3721 ttcaatacaa acagttccgg gtgcaataaa tatatcttct gctttatctg aagttcagga
     3781 agaggctgtt tctgcacttg ttatgttggg ttttgcggct ccggcagtta gaaaagtaat
     3841 atcaaaaatt gcagaagccg attcttcact tagtgtagag attataataa agaaagcctt
     3901 aaaaatgatg tagtcccttg aaaaaatcag tagaaaaaat atttcttatt ttagctttgt
     3961 tatgcggaat ggtgctcatt cctgtatggg ctgttgaacc tgtatctgaa gatgcaccga
     4021 ataacaccga gcttgcacaa gacacttcga agcctcgtta tcagacaaat aagatatatc
     4081 ctgagactca atccgattta gaagaatcgg ctcctattga tctgccggct ccggaaaata
     4141 ttaaaacaaa aatagattac gatccaacta caggtaaatt cttttttaat cctcaattag
     4201 gtgaagaaaa cgtatcaact ccattttatt tgaatagcga tgaatattat gattattctg
     4261 caaaaaaatc tttagaatca tattatctgt ctaagaatag ggaagaaaaa aaatctaagc
     4321 atcaattgtc tttgaccgaa atgaaattcg atataggtca ggctgataaa gtttttggtc
     4381 ccggaggtgt acaattaaaa ttacaaggat cagccgagct tgattttgga ttggatttta
     4441 aaaagttaaa ggatccatca ttgaacgagc gttctcaaaa tcccgctcca acatttgatt
     4501 ttgatgaaaa aattcaattg agtgtagatg gttctgtcgg agataaattg aagtttggct
     4561 taaattacaa cacagaggca acattcgatt ttgaccaatc tttactaaaa ttgcaatatg
     4621 aaggcaaaga agatgatatt ataaagaaaa ttgaagcagg aaatgttagt atgcctctta
     4681 ccggttcttt gataaccgga aataccagct tattcggttt taaggccgac ttgcaattcg
     4741 gtaaattaaa aattaatgct gtaatatcac aacaagaatc agaaggtaag acccttaata
     4801 ccagcggtgc acaaacaact gattatgaaa tacgagctga tgcttatgat gaaaacagac
     4861 actatttttt atctcactat ttcagagaac attatgatga atggatgtca gaactcccta
     4921 atatttcgtc aggcatatct ataggtgaaa ttgaagtatg gataacaaat aaaaatacca
     4981 ttaattacga taatacttca aatgttgttg gttttgctga tattgcagaa cctaatcgta
     5041 tatataattc ttcatttata tcaacaggca atccaacagt tcctgataac gctgcaaata
     5101 cattgtttgc ggatgtagtg caatcaattg gcggacttcc caatatttat gaggtcgaca
     5161 cttacctacg ttcatttacg atgcaaagca ccattgacta tgttaaattg gagggtgcaa
     5221 gaaaattgga atcttctgaa tataccatca acaaagaact tggttatata tctttaaaat
     5281 cggcattgaa taatgatgaa atgctggccg ttgcatatca atatacatac aaaggcaagg
     5341 tatacaatgt cggtgaattg tctaagggat ctgagccgac caaaccatta tctttaaaac
     5401 tattaaagtc tattgataaa tcaccatttg ttccaacttg ggatttgatg atgaaaaacg
     5461 tctatgcttt aggagcttat caagtacaaa gagataagtt tacttttaac attatgtata
     5521 ggaacgattc gaccggtatc gatttgcgtt acatatcaga aggacctatt gctaaaaagc
     5581 cattaatcaa ggttatgaat ttagaccgct tggattcgca tttgcaaaag acagctaatg
     5641 gtgatggtat attcgattat gtagaaggct atacgattat ttctcaaaat ggtagagtta
     5701 tattccctgt tttggaacca ttcggctcat acttaaaaaa gtcattaaac aacgatccta
     5761 tacttgtcga aaaatatgta tttcaggaat tatacgattc taccttgact gtggctcaag
     5821 aattagcaga aaagaataaa tttcatatgg tgggcgagta cagagcttcc tccggttctg
     5881 agatacgtct taatgccatg aatgttccaa aaggttctgt aaaggtaact gccggtggtc
     5941 gagaacttgt tgaaaatgtt gattatactg tcgattacat gatgggtgtt gtttcaatta
     6001 tgaatcaaga tttgatagat ctcggaactc ctattagtgt tacgatggaa agtcaatcaa
     6061 tgtttaatat gaagcgtaaa tcattaatcg gaacacattt ggattatgca tttaacgatc
     6121 attttaatgt tggtggtact atcatgtatc tttcagaaaa gccattaacc gagaaagtta
     6181 gttatggtga tgaccctgta gcaaatacta tttggggatt aaatacatca tatcgtaatc
     6241 aatttcaatc tgttacagat ttcttaaata agataccgat cttaaattta aaacaaccat
     6301 catctattgc ctttaatgct gaatttgcac aaatggtacc gggtacggca aaaggtgcaa
     6361 acggaaaggt ttatgttgat gattttgaat ctacgaagat aggatttgat gtacgttatg
     6421 catcaaattg gaaattatca agtactccat tgcgtttcga agagtctgct aatgataata
     6481 atattgagta tggaaagaat cgtgcattgt tatcatggta ttacattgat aatatgttta
     6541 atagaagtac gtcatctact ccatcatata ttagaaatga taaggaacag ttatctaatc
     6601 atttcgtccg tgagataagt gaacaagaga tattccctaa tcgagagttg gtttatggcc
     6661 agtctactta tattcagacc ttagatttag cttactatcc tgaggagcgt ggcccgtata
     6721 atgtatttga ccaaaacttt aatagcgaag gttatttaat agatccaaaa tcgaaatggg
     6781 gtggtataat gcgtaagttg gaaacaaccg attttgaatc aaacaatatc gaatatttag
     6841 agttttggat gattgaccca tttgtttata atgatggaac tgctaagggt ggtgatttgt
     6901 atttcaattt cggtgatatt tctgaggatg ttttaaaaga tggtcgtaaa ttctacgaga
     6961 acggtatgcc gttcgatggc tcaacagacg atgttgtaga aactgtatgg ggtaaggttc
     7021 ctagtaaaca atctctaaca tattcgttcg atagcgatgc ggcagcacgt aaacttcagg
     7081 ataccggttt aaacggattg cttactgaag aggagtttac ttt
//
LOCUS       DAOPID010000058         7032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_13737_length_7032_cov_3.719077, whole genome shotgun sequence.
ACCESSION   DAOPID010000058 DAOPID010000000
VERSION     DAOPID010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 7032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7032
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13737_length_7032_cov_3.719077"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="PK641_03130"
     CDS             <1..446
                     /locus_tag="PK641_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55378.1"
                     /translation="VISAGVTDLDSGLNILYTKYNNNIVIIPEKNLSNFGMVTLPLSI
                     VINGYNKDKSPNIAPEIKYKGEKCLSSSSVPSTGIKKGESSNNAHKKHKKARYLVAVV
                     LIAHNCTTTPPKIQIVDKAITRTTNKVIGVKVSGNSIGKFLRFLV"
     gene            complement(569..760)
                     /gene="xseB"
                     /locus_tag="PK641_03135"
     CDS             complement(569..760)
                     /gene="xseB"
                     /locus_tag="PK641_03135"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HPG55379.1"
                     /translation="MEKIKTYDEAYSQLEILTEEIKNGEIPLNLLTKKIKEAKALIEF
                     CQTSLAKVDNDVNDFLHNC"
     gene            complement(776..1936)
                     /gene="xseA"
                     /locus_tag="PK641_03140"
     CDS             complement(776..1936)
                     /gene="xseA"
                     /locus_tag="PK641_03140"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPG55380.1"
                     /translation="MEALSLYELNRTIKESIDELYPMSVWVKSEISRITENRSGHCYL
                     ELVEKDPNNDEVIAKASASIWKNTYKMLAPYFAMETGTALSQGMTVLIKVKPVFHEAY
                     SYSLNVTDIDPTYTIGDMSQKRKIVLARLKEESVFDMNRELTLNRAIRNIALVSSKTA
                     AGYEDFVNQLNNQEVKFNYNVELFEAVMQGDLTENSIINALDSIISSKTKFDIVVIVR
                     GGGAVSDLHWYDSYRLSYYCTQYPLPIITGIGHDRDISVLDMVAYTSLKTPTAAAQFI
                     VNRSISTYNDLKNLINEFSASVQSYIKNKQVELEMRKIDLKAAVSDLFVKKRHNLSMM
                     EQFLDLSSPSVILKKGYSISLKDGKVARCKDLHKGDCIKTILFDGNVESIIK"
     gene            complement(1936..2679)
                     /locus_tag="PK641_03145"
     CDS             complement(1936..2679)
                     /locus_tag="PK641_03145"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55381.1"
                     /translation="MKNRFLIIVLFLLPSVILAIEPIYTVKKYPNGQLKYEGYFIDGK
                     PIGEFKRYHENGNLSIIQNFDKVGNSSVKIYTGEGDFLAEGQYVGEKRDGEWKFYTKK
                     KALFIVEHYSLGKGTGEKLVYYEDGTIMNKMYYKNDLQDSVYLQYYQNGNVMAKFYYK
                     NGKIDGSFVSYYEAGELDRKGEYVNGKREGTWLIYKEDGKADEIIYKNGVSEQMNKMI
                     RAESDSSDVDRNFKDPEDYISNPSAYFER"
     gene            complement(2676..3587)
                     /locus_tag="PK641_03150"
     CDS             complement(2676..3587)
                     /locus_tag="PK641_03150"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009128281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPG55382.1"
                     /translation="MRNKPKSSGQKQFSLKVTEEEPLMEFLSLKLPDYSKTTLKSLLT
                     HRQLFLNGETMLVQHDYMLKPGDRIDIRSSKGERGHVLDHPSLKIIYEDDAILVVDKK
                     EGLLSVRTEKDGFETASHLLNTYMRAYGRGSHIYVVHRLDRDTSGVMMFAKTKEIQET
                     LRDNWNNLVQERTYIAVAEGVFEQTKGTIASYLTEDVQKKMHSSLKDNGGQYAVTHYE
                     VIAANKKYSLLKLNLETGRKNQIRVHLQSIGHPVAGDAKYGGSSSPAKRLCLHAETLK
                     FNHPVTKKLVAFEIPVPTSFLRLTGLK"
     gene            3782..4027
                     /locus_tag="PK641_03155"
     CDS             3782..4027
                     /locus_tag="PK641_03155"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HPG55383.1"
                     /translation="MNIKNVLAFVGGVAIGAAVGILLAPEKGKDTRAKIIAYLAEKGI
                     SKDRFEEIIAKAKAKISDFSNFDDVKAAVDEALNEQA"
     gene            4024..4401
                     /locus_tag="PK641_03160"
     CDS             4024..4401
                     /locus_tag="PK641_03160"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HPG55384.1"
                     /translation="MKLDPEYKQLLNDAKEYISLRYDLLRLEVLEKLSLILSLFILIV
                     ISVMLALAAFVYFSLAFAYYLKSLLGSMTPGLCIIGGIFILILILIVAFRKQFIINPL
                     IKRISKILFKDTDYQNETDNSKN"
     gene            4419..4706
                     /locus_tag="PK641_03165"
     CDS             4419..4706
                     /locus_tag="PK641_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55385.1"
                     /translation="MSINYSQIKNTDQIDRERRSLIRRIDKQKLAMDHNFEVAKSRFA
                     DNFSVWGIIKNISGSLFSFKTTSGQTISAIGLGYKIASSLYKRYKEKKASI"
     gene            4881..>7032
                     /locus_tag="PK641_03170"
     CDS             4881..>7032
                     /locus_tag="PK641_03170"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55386.1"
                     /translation="MKNHAIKRIANIAAFMTIPLLSFAISFTTSTKNPSCYSLADGSI
                     TVSVIGASGTVYYSLKADFSEQQIDNNIFLNLPAGLYDVYVKDDSGVTGPSIVQLVEP
                     DEISITATASPSACSNGEIEIQAQGGTQPYLYSLNNFTGDGQQTNIFRNLTAGNYTIY
                     VKDANGCLKTSATPVVVDDVKMSVGQPSTIQTKCAETADGSAITIVKGGVPSGSGNPY
                     VATLFKDNVAYPVTDILFTDMSGDTRITIGALSKGTYTGTISDQTGCAIPLSFVIGGP
                     AELSSILQNKTEVLCKGLSTGTINIISNGGTKPYTVTCNGGTVVTVENNTNISQLPAG
                     DYQISVTDANSCLDKAGIINVTINEPIDELTYSPNGNNVDCFGASTGKIFGEAKGGTL
                     PYIYNITNITTPYTAANTIGTFENLPMGNYTATVKDKNNCSVTSTDIIVGEPEEIVID
                     DITTNPAANVICKGDKTASVRFTLIGRTQIVAPTDTAIYYRVSLFNITEQIEVSSADL
                     KYTNRFHPVLTRIRYEQEPVIDPLTGDPAVDPETGDPITTRVAYKDTLWTDGCHEPTK
                     ELEIDDYTVDKKGFDCDDYVTVSSLGEAAYRISFFRGNCQIGNDIEFSVGRTGDVPAS
                     VKLNGMTPICDGTTITITPEIVSTPATNSYKWYLDGIQVGTTRLFEHEYIAAENNRVL
                     TLEATNRCGTTVSNAMSVHVWPRPTAVVETKEEFL"
BASE COUNT         2382 a         1281 c         1078 g         2291 t
ORIGIN      
        1 ttgtaatatc agcaggtgtt acagatttag actcgggatt aaatattcta tatacaaagt
       61 ataataacaa tatcgtaatt ataccggaaa agaatctaag caattttggt atggtaaccc
      121 tacctttatc tatcgttatc aacggatata acaaagacaa atcacctaat atagcacctg
      181 aaattaaata taagggagaa aaatgtctaa gttcgtcttc cgtaccatct acaggtataa
      241 aaaaaggcga aagctcaaat aatgcccata aaaagcataa aaaagcaaga taccttgttg
      301 ctgttgtttt gattgcacat aattgcacca caaccccacc aaaaatacaa attgtagaca
      361 aggcgataac aagaacaaca aataaagtta taggagtaaa agttagtggt aattccatag
      421 gcaaatttct tcgtttttta gtttagttca cgtaccaatt gatcatcaat agaaccaaca
      481 atgcaaatgt acacaaaaaa aactcacaga caatgaatgt tttgcatttt tgcttaaatc
      541 agatgataaa agactaatta ctaagacact aacaattatg caaaaaatca tttacatcat
      601 tatcaacttt agctaaggaa gtctgacaaa attcaatcaa agcttttgct tctttaattt
      661 tttttgtcag caaattcaat ggaatttctc cgtttttgat ttcttctgtc aaaatctcaa
      721 gttgggaata agcctcatca tatgttttta tcttttccat attgaacaaa ataatttatt
      781 tgattattga ctctacatta ccatcaaata agatagtctt aatacaatca cctttatgca
      841 aatctttgca ccgtgcaact ttcccgtctt ttaaacttat gctataacct ttttttagta
      901 taacagatgg tgaagataaa tctaaaaatt gttccatcat acttagatta tgcctttttt
      961 ttacaaacaa atcagatact gctgctttta aatcaatttt acgcatttct aattctactt
     1021 gtttgttttt tatataagat tgcacagatg cagaaaactc gtttattaag ttttttagat
     1081 cattatatgt agaaatagaa cgattaacta taaattgagc tgctgcggta ggtgttttta
     1141 aagatgtata cgcaaccata tcaagtactg atatatctct atcatgtcct atccctgtta
     1201 taattggcaa tggatattga gtgcaataat acgataagcg atatgaatca taccaatgca
     1261 aatcagacac tgcacctcca cccctaacaa ttacaactat atcaaactta gttttagaac
     1321 taattatact gtctaatgca ttaataatcg aattttctgt taagtcgcct tgcattacgg
     1381 cttcaaacaa ttcgacattg taattgaatt ttacttcttg attattaagt tgattgacaa
     1441 aatcttcgta accggctgca gtttttgaac taacaagagc tatatttctt attgctctat
     1501 ttaaagtcaa ttcacgattc atatcaaaaa cagattcttc tttcagcctt gctaaaacta
     1561 tcttccgttt ttggctcata tctcctatag tatatgtcgg atctatgtca gtcacattca
     1621 gactataact atacgcctcg tgaaatacag gttttacttt gatcagtacc gtcatacctt
     1681 gtgataatgc tgttcctgtt tccatagcga aatacggggc taacattttg tatgtatttt
     1741 tccaaataga agcagaagcc ttggcaatta cctcatcatt atttgggtct ttttctacca
     1801 attctaaata acaatgcccg gatcgatttt cggtaattcg acttatttct gactttaccc
     1861 aaactgacat tggatataat tcatctatag actctttaat tgtcctatta agttcatata
     1921 acgacaatgc ctccattaac gttcaaaata agcgcttgga tttgaaatgt agtcttccgg
     1981 atccttaaaa tttctgtcaa catcactact atcactctct gcccgtatca ttttattcat
     2041 ttgttcagaa acgccattct tatatattat ttcgtctgct tttccgtctt ctttataaat
     2101 aagccatgta ccttcacgtt ttccattaac atactcacct tttctatcaa gttcgccggc
     2161 ctcataatag gagacaaatg atccatcaat ttttccgttt ttatagtaaa atttggccat
     2221 aacatttcca ttctgataat attgtaggta cacactatct tgcaaatcat ttttataata
     2281 catcttgttc ataatagtac catcttcata atacactaat ttttcgcctg ttcccttacc
     2341 tagtgaataa tgttcaacta taaataaggc tttcttctta gtataaaact tccactcgcc
     2401 atcacgtttt tctcctacat actgcccttc tgccaagaaa tctccctcac cggtgtaaat
     2461 ttttactgac gaattaccta ctttatcaaa attctgaata atcgacaaat taccattttc
     2521 atgatatctt ttaaactctc ctatcggttt accatcaata aagtatcctt catacttaag
     2581 ttgtccgtta ggatattttt ttactgtgta aataggctct attgccaata ttacagatgg
     2641 caataaaaac aaaacaatta tcaaaaatct atttttcatt ttaatcctgt taatcgtaaa
     2701 aatgaagttg gaaccggaat ttcaaaagca acaagttttt ttgtaaccgg atggttaaat
     2761 ttaagtgttt ctgcgtgtaa gcacaaacgt ttcgcggggc tactactacc accatattta
     2821 gcatctcctg caaccggatg accaatactt tgcaaatgca cacgaatttg attctttctt
     2881 cctgtttcca gattaagttt caacaaagaa tattttttat tcgctgcaat aacttcgtag
     2941 tgagtaactg catattgtcc accattatct ttcaaacttg aatgcatttt cttttgaaca
     3001 tcttcagtta gatatgaagc tatagtccct tttgtttgtt caaaaacacc ttctgctact
     3061 gcaatataag tacgttcttg taccaaattg ttccaattat ctcttaaagt ctcttgtatt
     3121 tcttttgttt tagcaaacat catcacgcct gatgtatctc tatctaatct atgaacaaca
     3181 tatatgtggc tacctcttcc ataagcacgc atataagtat ttaacaaatg agatgctgtt
     3241 tcaaaaccat ctttttctgt tcttacggac agcagacctt cttttttatc tacgaccaat
     3301 attgcatcat cttcataaat aattttcaaa ctaggatgat ctaaaacatg tcctctttcg
     3361 ccttttgaag atcttatatc aattctatcg cccggtttaa gcatataatc atgctgcact
     3421 agcatagttt cgccatttaa aaacaactgc ctatgagtaa gcagtgattt caatgtcgtt
     3481 ttgctgtaat caggcaattt taacgacaaa aattccatca atggttcttc ttctgttact
     3541 tttaatgaaa actgcttttg acctgatgat tttggtttgt tccgcatatc tattttatat
     3601 ataagaacaa aggtacgttt ttttttcgat taacaatctg atataacaac tacactgcat
     3661 ttaaaaacaa aaattaaata ctattttatg taattaattt attgactttt atttggatat
     3721 acagcaaaat taaagtacat ctgccataat aataacttac aaaataaaag gagggactat
     3781 tatgaacatt aaaaatgtat tggcatttgt cggtggcgta gctatcggtg ctgcagtcgg
     3841 tatcttattg gctcctgaaa aaggtaagga cactcgtgca aaaataattg catacttagc
     3901 tgaaaagggc atctcaaaag atcgttttga agaaattatt gcgaaagcta aagctaaaat
     3961 ttcagacttt agtaattttg acgatgtaaa agctgctgtt gacgaggctt taaacgaaca
     4021 agcatgaaac tagatccaga atacaaacag ctactgaacg atgcaaagga gtacatatca
     4081 cttagatatg acttattgag attggaagtt ttagaaaaac tttcgcttat tttgtcgtta
     4141 tttatcctga tagttatatc agtaatgctc gctttagcag catttgtcta tttctcttta
     4201 gcatttgcat attatctaaa atccttatta ggaagtatga cacccggttt atgcataatt
     4261 ggaggaattt ttatactgat actcattcta attgttgcat tcagaaaaca atttataatt
     4321 aatcctttaa ttaagcgtat cagtaaaata ttatttaagg atactgatta tcagaacgaa
     4381 actgataatt caaaaaacta agataactga caatatatat gagcataaat tatagtcaga
     4441 taaaaaacac agaccagatt gatcgcgaaa ggcgatctct aatacgtaga attgataaac
     4501 aaaaacttgc aatggatcat aattttgaag ttgcaaaatc acgtttcgca gataatttct
     4561 ctgtttgggg aataattaag aatatatcag gaagtttatt ttcctttaaa accacatccg
     4621 gtcaaacgat atcggctata ggacttggct acaaaatagc ctcgtcacta tacaaacgtt
     4681 ataaagaaaa aaaagcttca atataaaaga acggacactc tatcgcttag aagtccgttc
     4741 gttttatttt atttcaatag gttttgcaaa tatattaaca tatcattcga aattatatag
     4801 tagttttaga taaaaaaacc ataaattata ttatctttgc aaaatatcaa aaaaagtata
     4861 taccaaatca atacactctc atgaaaaatc acgcaataaa gcgcattgca aatatagctg
     4921 cgtttatgac aattcctctt ttgtcgtttg caatttcatt cacgactagt acaaagaatc
     4981 catcttgcta tagcttggca gacggtagca ttactgtttc cgttataggt gcatccggaa
     5041 ccgtttacta ctcattaaaa gcagatttta gtgagcagca aatagacaac aacattttcc
     5101 tcaatcttcc cgcaggattg tatgatgtgt atgtaaaaga tgatagtggt gtaacaggac
     5161 cttctattgt tcaattagta gaaccggatg aaataagtat tacagctaca gcaagcccta
     5221 gcgcttgttc taatggtgaa attgaaatac aagctcaagg tggcacacag ccatatttgt
     5281 atagtttgaa taattttacg ggagatggac aacaaacaaa tatttttcgt aacctaacgg
     5341 caggaaatta caccatatac gtaaaggacg caaacggatg tttaaaaaca tcagcaaccc
     5401 ctgttgttgt agacgatgtt aaaatgtctg ttggtcaacc aagtacaatt caaaccaaat
     5461 gtgcagaaac agctgatgga agcgcaatta caattgtaaa aggcggcgta ccaagtggtt
     5521 ctggtaatcc atacgttgct accttattta aagataatgt ggcctaccct gttacagata
     5581 ttttatttac agatatgtca ggagacacaa gaataactat aggagctctt tctaaaggaa
     5641 catacaccgg tacaatatca gaccaaacag gttgtgctat tccattgtca tttgtaattg
     5701 gaggacctgc agaactatct tctattcttc aaaacaaaac tgaggtatta tgcaaaggtt
     5761 tatctacagg tacaatcaat ataattagta atggaggaac aaaaccttac acagttacat
     5821 gcaacggagg aacggttgta acagttgaaa ataatacgaa tatcagccaa ttaccagccg
     5881 gtgattatca aatttccgta actgatgcga attcatgtct tgataaagcg gggataatta
     5941 acgttacgat aaacgagcct attgatgagc taacttattc accgaatggt aataatgtag
     6001 attgctttgg agcatcaaca ggtaaaatat ttggtgaagc taaaggaggt acattaccat
     6061 acatttacaa tataacgaac ataactactc cttatactgc tgctaacaca ataggtacct
     6121 tcgaaaattt accaatgggt aattatacag ctactgttaa agataaaaac aattgttctg
     6181 tgacatctac agatattata gtcggagaac cggaagaaat tgtaatagat gatattacta
     6241 caaatccggc agctaacgta atatgtaaag gagacaaaac tgcatcagtt agatttacac
     6301 tgattggtcg cacacaaata gtagcaccta cagatactgc tatttactac agagtttctc
     6361 tatttaacat tacagagcaa atagaagttt cttctgctga cttaaaatat acaaatcgtt
     6421 ttcatcccgt attaaccaga ataagatacg agcaagaacc tgttatagat cctttgaccg
     6481 gcgatcctgc agttgatccg gaaacaggag acccgatcac aactagagtc gcgtataaag
     6541 acacattatg gacagatggt tgtcatgagc caactaaaga attagaaatc gacgactata
     6601 cagtagataa aaagggcttc gattgcgatg attacgttac agtttcgagc ttgggcgaag
     6661 ctgcttacag aatatctttc ttccgtggta attgtcaaat aggaaatgat atcgagtttt
     6721 ctgtaggtag gacaggagac gtacctgctt cagtcaaatt aaatggaatg actccaattt
     6781 gcgatggtac aacaattaca atcacccctg aaatcgtatc aacacctgca acaaattcat
     6841 acaaatggta cttggatggc attcaagtag gtacaactag actatttgag catgaataca
     6901 ttgctgctga aaacaataga gtattaacat tagaagccac taacagatgt ggaactacag
     6961 tgtcaaatgc aatgagtgta cacgtatggc caagacctac agcggtagta gaaactaaag
     7021 aagaattcct tt
//
LOCUS       DAOPID010000059         6991 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_13843_length_6991_cov_4.664360, whole genome shotgun sequence.
ACCESSION   DAOPID010000059 DAOPID010000000
VERSION     DAOPID010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 6991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6991
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13843_length_6991_cov_4.664360"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..1255)
                     /locus_tag="PK641_03175"
     CDS             complement(173..1255)
                     /locus_tag="PK641_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPG55387.1"
                     /translation="MKQSYIIILIISILLTSILIVGGIILNQKKFGKLPSGKRLERIE
                     KSPNYNNGKFTNLQPTQFIISSGGLFGAILDILFCKHPDLRPDSTIEAIKTNLHNINF
                     DEDFFIWFGHSSYLLQINAKKYLIDPVFFDASPFSFVNTPFKGSNIYKPDDMPDIDYL
                     IITHDHWDHLDYKTVTQLKERIKHVICPLGVGEHFEYWGFNSQQIIEMDWNEHTNLDS
                     NININCLPARHFSGRGLKSNKTLWASFVLQTPGFNVFIGGDGGYGKQFAQIGKQFPCI
                     NLAILENGQYNMNWKYVHTLPMYLKQETTDLNAQQIITVHHSKYSLAPHPWYEPLNNE
                     KELLHDSVNITIPIIGEIVLINSQKR"
     gene            complement(1281..2492)
                     /locus_tag="PK641_03180"
     CDS             complement(1281..2492)
                     /locus_tag="PK641_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
                     /protein_id="HPG55388.1"
                     /translation="MILHIDCNNFFVSCELVLHPESANRPAIVANNNESGGGIILALN
                     KEAKSIGLKRGTPIFKAKEILAKNSVTIFQADHYRYEKFSEQVMQCVKDLDIVLDFTQ
                     YSIDEFFGSLPIDDTEELKDCVQKIKNHINESTGIAVSCGCAQTYTLAKMATHYAKKY
                     AGYHGICIITPDKRERALSLLPIEDVWGIGRKTRDSMKFYGIGSALDFASCNESFVKR
                     NYGTTGLHTWLELRGTPCIKLDRPKWQQSISQSRTFAYMTDDINVLIQEISNYASACA
                     TKLRAQNTVCKSVMVIINTNRHRNDLPQYNNKAVIKLSTPCNDSPNIIKAAITALNQI
                     YLPKFQYKRAGVILSDISVNDAVQLNIFEAIYDGKQSKLMQTIDSINSKFGKEKIHLA
                     IQGVKKQGKQK"
     gene            complement(2496..3326)
                     /locus_tag="PK641_03185"
     CDS             complement(2496..3326)
                     /locus_tag="PK641_03185"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HPG55389.1"
                     /translation="MNKKFNILLFIGSMFILASILLGMKFLHVESLSAQIPIKTKINA
                     PHIQKNNLEIPGKLIGRKEQIIRHIGYTVSYNEDWLIPNWVAYELTPQETYGQTPRAK
                     DFMPDPDVIGHSATTEDYKRSGYDRGHMAPAADMKWSKQAMDECFYLSNICPQNNNLN
                     GGDWKELEEKIRYWASTGDTLYIICGPLMAKTTQTIGANRVSVPAAFFKVILRKNANK
                     IAAIAFMMPNRSGHKALNTYTMSVEDMEIISEMDFFKVLPDDIETAIESNYCEEDWNL
                     "
     gene            complement(3323..4027)
                     /locus_tag="PK641_03190"
     CDS             complement(3323..4027)
                     /locus_tag="PK641_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011202221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPG55390.1"
                     /translation="MSGILYLIPNTLGECELNTVFPTANADIIRRIKHFIVEDIRTTR
                     RFLKKVDKNIDIDSLNFYELNGHTPKENISGYLQALSDENDMGIISEAGCPAVADPGA
                     DVVAIAQEKGYKVVPLVGPSSILLSIMGSGFNGQSFAFLGYLPIDAAERLKAIKKMET
                     RIYNEHQTQIFIETPYRNTKLAEELVQNCQASTKLCIAVDITTPTESIQTRTIKAWKS
                     NIPQLDKRPCIFLLYK"
     gene            4107..5408
                     /locus_tag="PK641_03195"
     CDS             4107..5408
                     /locus_tag="PK641_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA cytosine-C5-methyltransferase"
                     /protein_id="HPG55391.1"
                     /translation="MQVPQDFILQMQDILKDETPDFLLTLDEKSPVSVRLNNKLKLNP
                     DYLKVEHCKNAFYLPQRPQFTLDPWLHAGVYYVQEASSMYLEKIVEKYVKSDVNILDL
                     CAAPGGKSTHLSSLISPNSLLVSNEIMPQRAFVLAENMIKWGDANTIVCNNKPKDFSF
                     ADSFFDVILADVPCSGEGMFRKEPKAIEDWSLNNVAMCAQRQKQIISDVWNTLKENGL
                     LIYSTCTFNTQEDEDNVRWIESELGAECMESKHFYFHKNRGEGFFIAALRKISNNNNT
                     CRIKVQKTEKTHALLSNLTNADDLTIIRRTDSVDAVPHNKLSEYKFLSEKLNILKCGT
                     KLCTQKGKDEIPDIQLALSKHIDTSKVNSFDVDWLTSIKYLKKEALRLDNAPIGYVLL
                     SFRSVPLGWVKNIGNRCNNLYPNEWRIRMNNIPNEQCELPF"
     gene            5390..6139
                     /locus_tag="PK641_03200"
     CDS             5390..6139
                     /locus_tag="PK641_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006046291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPG55392.1"
                     /translation="MRITFLGTGTSSGVPHINCDCETCLSSDPKDKRLRASVMVQEGH
                     NNFLIDCGPDFRQQILAHKFRNIDGVILTHEHFDHVGGLDDLRPFHDIHIYAEKRVCN
                     IVRSNMPYCFDGKKYPGVPKLNLVEINENPFCINDVKIIPIRFYHAELPILGYRIGNF
                     AYLTDISRIEKSEIQKLEGVKVLVVDALRERPHFSHFSLSEALAFIELLKPSKAYLTH
                     ICHQMGKTADVQPKLPKGVYLAYDGLEVNVE"
     gene            6265..6576
                     /gene="rplU"
                     /locus_tag="PK641_03205"
     CDS             6265..6576
                     /gene="rplU"
                     /locus_tag="PK641_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013063597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPG55393.1"
                     /translation="MYAIVEIAGQQFKVEKDQKVFVHRLNEERGAKVEFEKVLLIDAD
                     GKVKVGAPTVEGAKVLAEVVSHVKGDKVIVFKKRRRKGYRVRNGHRQCFTEILIKDVV
                     A"
     gene            6598..6864
                     /gene="rpmA"
                     /locus_tag="PK641_03210"
     CDS             6598..6864
                     /gene="rpmA"
                     /locus_tag="PK641_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPG55394.1"
                     /translation="MAHKKGQGSSHNGRESESKRLGIKIFGGQIAKAGNILVRQRGTV
                     HHPGENVGIGKDHTLFALKAGVVVFRKKKEDRSYVSIEEVAEQA"
BASE COUNT         2184 a         1120 c         1280 g         2407 t
ORIGIN      
        1 acaaaaaaaa atataaatca aaaattttgg tgtttttatt tttaataata aaagacgccg
       61 gcatcatgct attttttttg tatttgtatg aatagcaagt cattaaatta ttatattatg
      121 acagttacca gtatgatttt atttgcatac ttttttgtag tcataatata cactagcgct
      181 tttgtgaatt gatgagaaca atttctccga taattggaat cgtgatattt accgaatcat
      241 gcagtagctc cttttcgttg ttaagaggct cataccaagg atgcggtgct aatgaatatt
      301 ttgaatgatg tacggttatt atttgttgcg catttaagtc agttgtttct tgtttaagat
      361 acatgggtaa agtgtgtaca tatttccaat tcatattata ctgaccattc tctaaaatcg
      421 ccagattgat gcagggaaat tgcttgccta tttgtgcaaa ttgcttgcca tatcctccgt
      481 cgccacctat aaaaacatta aaccccggcg tttgtagcac aaaagatgcc cataaagttt
      541 tatttgattt taagcctcgt cctgaaaaat gccttgcagg taaacagttt atattgatgt
      601 tactatctaa attggtatgc tcgttccaat ccatttctat aatttgttgc gaattgaatc
      661 cccaatattc aaaatgctcg cctacgccta atggacatat tacatgtttt atacgttctt
      721 ttaattgcgt tactgttttg taatcaagat gatcccagtg gtcatgagtg atgattaaat
      781 aatcaatatc aggcatatca tcaggtttgt atatattact tcctttaaat ggagtattta
      841 caaatgaaaa tggtgatgca tcaaaaaata caggatctat taaatatttc ttggcattta
      901 tttgtaataa atatgaagaa tgtccaaacc agataaaaaa gtcttcgtca aaatttatat
      961 tatgtaaatt agtttttata gcttcgattg ttgaatcagg acgcaaatcc ggatgtttac
     1021 aaaataaaat atctaatatt gctccgaata aaccaccgga tgaaattatg aattgagtgg
     1081 gttgtagatt tgtgaatttg ccattattgt aattaggtga tttttcaatt cgttctaatc
     1141 gcttgccgct gggaagtttt ccaaactttt tttgatttaa aattataccg ccaacaatta
     1201 atatgctagt caatagaata gagattataa gtattattat gtaactttgt ttcattgtat
     1261 ataatattga gtgaaatcta ttatttttgt ttgccttgtt ttttgactcc ttgtatagct
     1321 aagtgaatct tttcttttcc gaatttactg tttatactat cgatggtttg cattaatttg
     1381 ctttgcttgc catcataaat agcttcaaat atatttaatt gaacggcatc atttacactg
     1441 atgtcagata aaatgactcc tgcacgtttg tattgaaatt ttggaagata aatttgattc
     1501 agagctgtta ttgcagcttt aataatgttt ggagagtcgt tgcaaggggt tgacaacttt
     1561 attacagctt tgttgttgta ttgtggtaga tcgttgcgat gtctattggt gtttataata
     1621 accattaccg atttgcaaac cgtattttga gcccgtaact ttgtggcaca tgcacttgca
     1681 taattgctaa tctcttgtat aagtacatta atatcgtctg tcatataagc aaaggtacga
     1741 ctttgcgaga tgctttgttg ccattttggg cgatctaatt ttatgcaagg agttccgcgt
     1801 aattctaacc atgtgtgtaa gccggtggtt ccataattac gtttaacaaa agattcgttg
     1861 catgatgcaa aatctaaagc acttcctatt ccataaaatt tcatactatc tcttgtctta
     1921 cgtccaatgc cccatacatc ttctatgggc aaaagactta gtgcgcgttc tctcttgtcg
     1981 ggagtgatta tgcaaattcc atggtatcct gcatattttt ttgcataatg agtagccatt
     2041 ttagctaagg tgtatgtttg cgcgcatcca caactaacag ctatgccagt agattcgttt
     2101 atgtggtttt tgattttttg tacacagtct tttagttctt ctgtatcatc aataggcagg
     2161 cttccaaaaa actcatcaat agaatattga gtaaaatcca aaactatatc taaatctttt
     2221 acacattgca tcacttgttc tgaaaatttc tcatacctat aatggtctgc ctgaaaaatc
     2281 gtgactgaat ttttagccag aatctctttg gccttaaaga taggagtccc acgttttaag
     2341 ccaatagatt ttgcttcctt gttaagagct aaaatgatgc caccgcctga ttcgttatta
     2401 tttgctacaa tagcaggcct attcgctgat tcgggatgta aaaccagttc acaactaaca
     2461 aaaaaattat tacagtcaat atgcagtatc atagactata aattccaatc ttcttcacaa
     2521 taatttgatt caatagcagt ctcaatgtcg tcaggtagga ctttgaagaa atccatctca
     2581 gagataattt ccatatcttc aacgctcatt gtatatgtgt ttaatgcctt atgcccactt
     2641 ctgttaggca tcataaaggc tattgctgca attttatttg cattttttcg taatataact
     2701 ttgaaaaaag cagccggaac cgaaactcta ttggctccaa tagtttgtgt agtttttgcc
     2761 attagtgggc cacatataat atataatgta tcacctgttg atgcccaata acgaattttt
     2821 tcttccaatt ctttccaatc acctccattc agattgttgt tttgtgggca aatattagat
     2881 aaataaaaac attcatccat tgcttgttta ctccatttca tatctgcagc cggtgccata
     2941 tgaccgcggt catagcctga tcgcttgtaa tcttcggtag ttgcagaatg tcctataaca
     3001 tcagggtcgg gcatgaaatc tttagctcta ggggtttgtc cgtaggtctc ttgaggagtt
     3061 aattcgtaag ccacccaatt cggaatcagc caatcttcat tataacttac ggtgtatcct
     3121 atatgacgaa taatttgttc ttttctgcca ataagctttc ccggaatttc aagattgttt
     3181 ttctgaatat gtggtgcatt aatttttgtt ttgataggta tctgtgctga taaagattcg
     3241 acatgcaaaa atttcatgcc aagtagaatc gatgcaagaa taaacattga ccctataaac
     3301 aataatatgt tgaacttttt attcatttgt ataacaagaa gatacacgga cgcttgtcta
     3361 attggggtat attagatttc catgctttaa tagtgcgtgt ttgtattgat tctgtaggag
     3421 ttgttatgtc aacagcaatg cataatttgg ttgatgcttg acaattttga actaattctt
     3481 ctgccagctt tgtgtttcta tatggagttt ctataaatat ttgcgtttga tgctcattat
     3541 atattcttgt ctccattttt tttattgcct taaggcgttc agcggcatca ataggaaggt
     3601 atcccaagaa agcgaaactt tgaccattaa atcccgaacc cattatagaa agaagaatgg
     3661 atgaaggtcc tactaatgga actaccttgt atcctttttc ttgagcaatt gcaacaacat
     3721 ctgcgcccgg gtctgcaaca gcaggacaac ctgcttcaga aataattccc atatcatttt
     3781 catcagaaag agcttgtaga tagccactta tattttcttt tggagtatga ccattcaatt
     3841 cgtaaaaatt caagctgtca atatctatgt ttttatccac cttttttaag aatcttcggg
     3901 tagttctaat atcttctaca ataaaatgtt tgatacgtct tattatatct gcgttagctg
     3961 tagggaacac ggtgtttaac tcgcattcac caagcgtatt aggtattaaa tatagtattc
     4021 cggacattgt tttttgttgt tgaatagaca aagatagtgc aaaatacgat attcactttc
     4081 aactaaataa attatctttg cgacttatgc aagtacctca agatttcata ctgcaaatgc
     4141 aggatattct gaaagatgaa acacctgatt ttttattgac tttagatgaa aaaagtcctg
     4201 taagtgtgcg tcttaataat aaattaaaat taaatcccga ttaccttaaa gttgaacatt
     4261 gcaaaaatgc tttttattta ccccaaaggc cacaatttac tcttgatcct tggttacatg
     4321 ccggagttta ttatgtgcaa gaggcatcgt caatgtatct cgaaaaaatt gttgaaaagt
     4381 atgtcaaatc ggatgttaat attcttgatt tatgtgctgc accgggtggt aaatctactc
     4441 atttgtcgtc tttaataagc cccaatagtc tgttggttag caacgagata atgcctcaaa
     4501 gagcattcgt tttggctgaa aatatgataa aatggggtga tgccaataca attgtatgta
     4561 ataataaacc taaggatttt tcttttgctg attcgttttt tgatgtgata ttggcagatg
     4621 tcccatgttc gggcgaagga atgtttagaa aagaacccaa agcaatagag gattggagtc
     4681 tgaataatgt tgcgatgtgc gctcaacgtc aaaaacaaat tattagtgat gtgtggaata
     4741 ctctgaaaga gaatggttta ttgatatata gtacttgtac ttttaatact caggaagatg
     4801 aggataatgt gagatggatt gagtcggaat taggagcaga gtgtatggag tcgaagcatt
     4861 tttattttca taagaatagg ggtgaaggtt tttttatcgc tgctttgaga aaaatatcta
     4921 ataataataa tacctgtcgt ataaaagttc agaaaacgga aaaaacacat gctttattat
     4981 ctaatcttac aaatgctgat gatttgacaa taatccgcag aactgatagt gttgatgcag
     5041 ttccgcataa taaactatct gagtataaat ttttgtccga aaaattgaat atattaaaat
     5101 gcggtacaaa attgtgtacg cagaaaggga aagatgaaat tcctgatatt caattggcat
     5161 tatcaaaaca tattgataca tcaaaagtaa atagctttga tgtagattgg ttaacatcaa
     5221 taaaatactt aaaaaaagag gcattacgtt tagataatgc acctatcgga tatgtgttgc
     5281 tcagctttcg ttcagtgcca cttggctggg ttaagaatat aggaaataga tgtaataatc
     5341 tgtatcctaa cgaatggcga ataagaatga ataatatacc taatgaacaa tgcgaattac
     5401 ctttttagga acaggcacat ctagtggtgt tccacatatc aattgcgact gcgaaacctg
     5461 tctttcgtct gatcctaaag ataagcgttt acgtgcatct gttatggttc aagaaggtca
     5521 taacaacttt cttatagatt gcggacccga ctttagacag caaatcctgg cgcataaatt
     5581 tcgcaatatt gatggtgtta tactaacaca cgaacacttt gatcatgttg gtggattgga
     5641 tgatttacgc ccatttcacg atatacatat ttatgcggaa aaacgtgttt gtaacatcgt
     5701 taggtctaat atgccatatt gttttgatgg taaaaaatat ccgggagttc ctaaacttaa
     5761 tttggtcgaa ataaacgaga atccattttg tattaacgat gttaagatta ttccgattcg
     5821 tttttaccac gctgaattac ctattttagg atatagaatt ggtaatttcg catacctcac
     5881 agatataagt agaatagaaa aatcggaaat acaaaaacta gagggtgtta aagttcttgt
     5941 agttgatgca ttacgtgaaa gacctcattt ttcacatttc tctttatctg aagcattggc
     6001 ttttattgaa ctgttaaaac catccaaagc atatttgact catatttgtc atcaaatggg
     6061 gaaaaccgca gatgttcaac cgaaattgcc taaaggtgtg tatttggctt atgatggctt
     6121 agaagtaaat gtggagtaaa attaattgaa aaagtttgtg cgttataaaa aagtttctta
     6181 actttgcggc ctgtaatttc taaaaggtaa gacaagatgc ttttaagcac acctcaggaa
     6241 tgtacctata aataatttta tatcatgtac gcaattgtag agatcgcggg acaacaattc
     6301 aaggttgaaa aagaccaaaa agtatttgtt caccgtttga atgaagagcg cggcgcaaaa
     6361 gtagaatttg aaaaagttct tttgattgat gctgacggaa aagtaaaggt tggtgctcct
     6421 accgtagaag gcgcaaaagt tttggctgaa gttgtttctc atgttaaagg tgataaagtc
     6481 atcgttttca agaaacgtcg tcgtaaaggt taccgtgtaa gaaacggaca ccgtcaatgc
     6541 tttacagaga ttttaattaa agatgttgtc gcttaattta tagaaaggaa ttagaaaatg
     6601 gcacataaaa aaggacaggg tagttcacat aatggtcgtg agtcagaaag caaacgactt
     6661 ggaattaaga tatttggcgg tcaaattgct aaagcaggca atatcttggt aagacaacgt
     6721 ggtacagttc atcatccggg tgaaaatgta ggtatcggta aagatcatac attattcgca
     6781 cttaaagccg gtgttgttgt atttagaaag aaaaaagaag atcgttcata cgtttctata
     6841 gaagaagtag cagaacaagc atagttctgt tcgttataac gatatatcat aatagaaatg
     6901 gaggtactta tgtatctcca tttttttgtc ataaaattac tgtgagatgt ttgagcttat
     6961 ttatctgtct gataatgcaa aattccgtat c
//
LOCUS       DAOPID010000060         6966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_13929_length_6966_cov_4.363044, whole genome shotgun sequence.
ACCESSION   DAOPID010000060 DAOPID010000000
VERSION     DAOPID010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 6966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6966
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13929_length_6966_cov_4.363044"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /gene="ribB"
                     /locus_tag="PK641_03215"
     CDS             complement(<1..696)
                     /gene="ribB"
                     /locus_tag="PK641_03215"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HPG55395.1"
                     /translation="MPLNTIKEAIEDFKKGKFLIVVDDEDRENEGDFIIAAEKITPEK
                     VNFMLKNGRGVLCAPITEERCQELELDRQVSNNTSILGTPFTVTIDKIEGCTTGVSAA
                     DRAATIKALADPTSTPNTFGRPGHINPLYAKIKGVLRRPGHTEAAIDLARLAGMYPAA
                     ALIEIMNEDGTMARMPDLQIVAEKFKIKIITIKDLIAYRIQLESIVEKGVEVHLPTQY
                     GNFNLIPFRQKSNG"
     gene            complement(687..2180)
                     /locus_tag="PK641_03220"
     CDS             complement(687..2180)
                     /locus_tag="PK641_03220"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPG55396.1"
                     /translation="MPLIKQIHKYVITSFLKTFAITFFGSVFVLVMQFMFRYVDDLVG
                     KGVGIGVLAEFFLYAALSIIPIALPLSILLGSLMTYGNLGERFELLAMKAAGISLFHS
                     MRSLIVLISCISIASLFFADNVLPKTQVRMWTLLFSIREKSPELDIPEGSFYSGISGR
                     NLYVGDKEGHLLKDIIIYDYSRGFNNTSIIIADTGTIQLSKNKQYLMLSLTNGETFEN
                     MHQGNSSGDNKIIPYRRETFKQKNIVIDFDANFKEMSSEFLEGQYVSKNTAQLQHTID
                     SINIRLDSTTTVYKEKSVNRNYFENGGNAERIVKAIDTTDIQKYDYKIVYYQLDSLKK
                     MTLIENALSRVTSVNSEILYRESAYDWDMGEIRKHAIEWNKRFTLSFACLIFLFIGAP
                     LGAIIRKGGMGIPIVISTILFIVYYIIDNTGYKMAREGIWPVPAGMWLSAAVLLPLGI
                     LLTYMAATDSTFMSGEAMMIAIKKFFRKENPIFVHCKKIKQKLKLCR"
     gene            complement(2184..2651)
                     /locus_tag="PK641_03225"
     CDS             complement(2184..2651)
                     /locus_tag="PK641_03225"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HPG55397.1"
                     /translation="MSKTLQYINSKILTGYRKELVEKAKSAALKAYAPYSNFKVGAAV
                     LMSDGQIYTASNQENAAFGESICAERVTLLYATANNPDVAPKAMAIAAFKNGNFTEQI
                     ISPCGACRQVLAETEKRYNSPIELILYSTNESVIISSAADLLPLSFSYGIMEQ"
     gene            2758..4032
                     /locus_tag="PK641_03230"
     CDS             2758..4032
                     /locus_tag="PK641_03230"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPG55398.1"
                     /translation="MQKKILIITYYFPPSGGSGVQRWLKFVKYLPEFNIQPIVLTVDE
                     KYASYPQYDNSLMTDVSKDLRIEKTKTCEILSLYKRLSPTKEVPYGGFSNEKKPNLFQ
                     KLSRFLRGNFFLPDPRRGWNKYAYKKACEIIEQEHIETIITTSPPHSTQLIGLRLKKR
                     FPMIKWIADLRDPWTEIYYNKDLYQTKLARHINRHYEIQVLKTADKIITVSESCKSMF
                     EKKVDIKEKINVISNGYDEDDFANVKTNKDNSNFVISYIGVLSPQYEIDTFIAAVKQL
                     SKTIKQKLLIRFVGQVYQGSKDKLDNLGVKIEYVDYVPHKTAVSYMLSSDILLLIIPN
                     ISDNKGILTGKLFEYIAAKRQIALVGPVDGDAANIINKSGIGDVFAYGESSSLSNFIE
                     RVYNNEICCLNYNSKCYSRKFLTQQLSEIIAD"
     gene            complement(3997..4935)
                     /locus_tag="PK641_03235"
     CDS             complement(3997..4935)
                     /locus_tag="PK641_03235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPG55399.1"
                     /translation="MDLRISILIPTYNQPLYIKRSVDSALAQDYPNIEVIVSDDSSSD
                     DTKNILEEITDKRFKYIRNNPRLGRVENYRTCLYKYSTGSWALNLDGDDYLTDPHFIS
                     DAVNLIESFNDKNLVFVQAGGYLIDKDGSKTGQRMPKIKTEHCIVSGKQYVCDFAKKR
                     RFLHLTSLYNTATAKSIDFYRYDILSTDLESFLRLALLGNVALLNKNVGVWFQHGKNT
                     SSSSSINKKLENTLWIDSVCDFALQTKTLTKFKANIWCYIVKQQELTGHFMVEISESK
                     NRLKTLWWIFVHYPATFFFPVFVKKLIGYNFRQLLG"
     gene            complement(4938..6143)
                     /locus_tag="PK641_03240"
     CDS             complement(4938..6143)
                     /locus_tag="PK641_03240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPG55400.1"
                     /translation="MTNRTLYLFTDSWPYGNGESFLQDESHFLSKTFSVVRVIPLHKY
                     NGIMRKIPANFVVMKSLLNEDPKHKIALLKAGVFNSSPLFFACKLFFKEKAYKNRKRI
                     WNFMTSLLVFRSAYKSFKALPSSNDLLYFYWGDKSINMLPFLKQKFKTKAIARFHGSD
                     LYEEVKGGYIPFRKWVLPYLDMAAPISEYGHEYLKKYGFLCPNRIETSRLGVFDNGLN
                     PTASNDNTLHLLTCSNIIKIKRLSLLLEAVMCLPFNVKWTHIGSGPLLKNLKEQSKQC
                     PDNIKIDWMGQQNHEFVMQLYAKQHFDIFVNVSETEGIPVSIMEALSFGIPVLATKVG
                     GVAEIVDENVGYLVSKDIKSDELASLLTNFYENKANAELRNNARKRFETMCDANENYS
                     HFCSTIENL"
     gene            complement(6140..>6966)
                     /locus_tag="PK641_03245"
     CDS             complement(6140..>6966)
                     /locus_tag="PK641_03245"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPG55401.1"
                     /translation="EYFQFSSHMLGTSLLTVIFNNIYTFLIGKFYSINQVGYYTQGYK
                     FSDSVNFTFLAILGTSYNLFAKIQHQQERIKRILGELVKNTSIVTIPITMFLIASAKP
                     LFFVLFGTKWLDSVPFFQLICAANLFAPIYQINIHSLNSMGKSHITFRMELFKRVLIL
                     TSILTCFTYGINTMLYGYILSCWIAFICSVLCIYKFVGNNGKQQFTQVLPGIIIGLAV
                     ALLVIGTSFISNNLYLTLTLQVLFTFALYIIIIRVFFKNLFLRLIAYIKTKKLNFK"
BASE COUNT         2300 a         1163 c         1138 g         2365 t
ORIGIN      
        1 accatttgat ttttgacgaa atggaattaa attaaaattt ccatattgag taggtaagtg
       61 aacttcaact cctttttcta ctattgattc taattgaatt ctgtacgcga ttaaatcttt
      121 aatggtgatg atttttattt tgaacttttc ggcaactatc tgcaaatcag gcatacgagc
      181 catcgttcca tcttcattca ttatctcgat taaagctgca gccggataca tacctgccaa
      241 acgagctaaa tctattgcag cttcagtatg tcctggtctg cgtaatacac ctttaatttt
      301 tgcatataaa gggttaatat gacctggacg accaaatgta ttaggcgttg aagtaggatc
      361 agccagggct ttaattgttg ctgcacgatc tgctgcagaa acacctgttg tgcatccttc
      421 aatcttatca atggtaacag taaacggagt tcctaatata gatgtgttgt tactgacctg
      481 acggtctaat tctaattctt gacagcgctc ttctgtgatt ggtgcgcata atacaccacg
      541 cccgttcttg agcataaaat taactttttc tggtgttatt ttttcagctg caattataaa
      601 atcgccttca ttttcgcgat cttcatcatc tacaacaatt agaaatttac cctttttaaa
      661 gtcttcaatg gcttctttaa tggtgtttaa cggcataatt taagtttttg tttaattttc
      721 ttacaatgta caaagatagg attttctttg cgaaaaaact tcttaatagc aatcatcata
      781 gcttcacccg acataaaagt tgaatcagtt gcagccatat atgttagcaa tattcccaat
      841 ggaagcaaga ctgcagcact tagccacatt cccgcaggta ccggccatat gccttctcgt
      901 gccattttat aaccggtatt gtctattatg taatatacaa taaatagaat tgttgatata
      961 acaatcggga ttcccatgcc gcccttacgt atgatagctc caagaggtgc acctataaat
     1021 aaaaagatta agcaggcaaa agatagggtg aatcgtttat tccattctat tgcgtgcttt
     1081 ctaatctctc ccatatccca atcatatgct gattccctgt ataaaatctc actgttaaca
     1141 ctagtaactc ttgaaagagc attttcaatt aatgtcatct tttttaaact atcaagttgg
     1201 taatatacaa ttttataatc atatttttgg atatcagttg tatcaattgc ctttactatt
     1261 ctttctgcat taccgccatt ttcaaagtag ttacgattaa cacttttctc tttgtaaaca
     1321 gttgtagtag aatccaatct aatattaatg gagtctattg tatgttgcaa ctgagctgtg
     1381 tttttactaa catattgacc ttccagaaat tcggaactca tttctttaaa attggcatca
     1441 aaatcaataa caatattttt ttgtttaaag gtttctcttc tgtatggaat gattttgttg
     1501 tcgcctgaag aattcccttg atgcatattt tcaaatgttt caccatttgt taggcttaac
     1561 attaaatatt gtttgttttt agataattga attgttcctg tatctgcaat aatgatgctt
     1621 gtattattga atccacgaga ataatcgtat attataatat cttttagcaa atggccttct
     1681 ttatcgccaa catatagatt tcttcctgat attccgctat aaaatgatcc ttcaggtata
     1741 tccaattccg gtgatttttc gcgtatagag aatagtagag tccacattcg gacttgggtt
     1801 ttaggcaata cattatctgc aaaaaagaga ctcgctatag aaatgcatga aattaataca
     1861 attagtgagc gcatactatg gaataaagaa attccggcag ccttcatagc taataactca
     1921 aatcgttctc ctaaatttcc ataagtcatt agagaaccta acaatattga tagaggtagt
     1981 gctattggaa taattgataa tgctgcatat aagaaaaatt cggctaaaac acctatacct
     2041 acgcctttac caaccaaatc gtcaacatat ctgaacataa attgcatgac caatacaaaa
     2101 acacttccaa aaaacgttat ggcaaatgtt tttaaaaaac tagttattac gtatttatgt
     2161 atttgtttta ttaacggcat gatttattgt tccattattc cgtatgaaaa tgaaagcgga
     2221 agtaaatctg cagcagaact aataataaca gattcgtttg tactatacaa gattagttca
     2281 atgggggagt tataacgttt ctccgtttct gcaagtactt gtctgcaagc accgcaaggg
     2341 ctaataattt gttcagtaaa attaccattt ttaaatgcag caattgccat tgctttgggt
     2401 gcaacatcag gattatttgc ggtggcgtat agtaaggtaa cacgttctgc gcatatagat
     2461 tcaccaaaag cggcattttc ttgattggat gctgtgtata tttgtccgtc agacattaac
     2521 acggccgcac caactttaaa attagaataa ggagcgtatg ctttaagagc tgcacttttt
     2581 gctttttcaa caagttcttt gcgatatcct gttaaaattt tactattaat atattgcaag
     2641 gttttactca taaatttaat cttacaaaat tacacaaaat aaactgaaaa cacaatcata
     2701 tgatgcttaa taatagcgaa atgaagagca ttattatttt tttgttacct ttgcattatg
     2761 caaaaaaaga tattaataat tacatattat tttcctccaa gtggtggatc cggagtgcaa
     2821 cgatggctca aattcgttaa gtatctgccc gaatttaata ttcagccaat agttcttacc
     2881 gttgatgaaa aatatgcttc atatccacag tatgacaatt ctttaatgac tgatgtgtct
     2941 aaagatttaa gaattgagaa aacaaaaact tgcgaaatac tatctttata taaaagatta
     3001 tctcctacaa aagaagtgcc ttatggaggc ttttccaacg agaaaaaacc taatcttttt
     3061 caaaaactgt cacgtttttt aagaggcaat ttctttttgc ctgaccctag gagaggatgg
     3121 aataaatatg catataagaa agcttgcgag ataatagagc aagagcatat tgaaaccatt
     3181 ataacaacaa gtccaccgca ttcaacacaa cttataggtt taagattgaa gaagcgtttt
     3241 cctatgatta aatggattgc tgatttacgc gacccttgga ctgaaatcta ttataataaa
     3301 gatttgtatc aaacaaaact tgctcgtcat attaacagac attacgaaat tcaagttttg
     3361 aaaaccgccg ataagataat tacagtcagc gaatcatgca aaagcatgtt tgaaaaaaaa
     3421 gttgatataa aagaaaaaat aaatgtaata tcaaacggat acgacgaaga cgattttgcc
     3481 aatgtaaaaa cgaataaaga taattcaaat tttgtgattt catatatagg agttttaagt
     3541 ccgcaatacg aaattgatac atttattgct gccgtaaaac aattatcgaa aacaattaaa
     3601 caaaaattat tgatacgttt tgtaggacaa gtttatcagg gtagtaaaga taaattagac
     3661 aatttaggtg taaagattga atatgtagat tatgtcccac ataaaacggc tgtttcgtat
     3721 atgttatcat ctgatatttt gttgctgata attccgaata taagtgataa caaaggaatc
     3781 ttaaccggta aattatttga atatattgcg gcaaaacgac aaatagcttt agttggacca
     3841 gttgatggtg acgctgcgaa tataattaat aaaagtggaa ttggcgatgt gttcgcgtat
     3901 ggagaaagct cgtctctatc aaattttatt gaaagagtat ataacaatga aatttgctgt
     3961 ttaaattata attcgaagtg ttattctcgc aaatttttaa cccagcaatt gtctgaaatt
     4021 atagccgatt aattttttca caaatacagg aaagaaaaaa gtagccggat agtgaacaaa
     4081 aatccaccat agcgttttca gtcggttttt actttcgctt atttcaacca taaagtggcc
     4141 tgttaattct tgctgcttta caatataaca ccaaatgttt gctttaaatt ttgtcagcgt
     4201 ttttgtttgt aaagcaaagt cgcaaacaga atctatccat aacgtgtttt ctaatttttt
     4261 atttattgat gaagatgacg atgtgttttt gccatgttga aaccatactc caacattttt
     4321 attaagtaat gccacattac ctagcaaagc aaggcgtaag aatgattcta agtcggtaga
     4381 taagatatca tatctataga aatcaatgct tttagcagtg gcggtattat ataaacttgt
     4441 aagatgcaag aatctgcgtt ttttagcaaa atcgcaaaca tattgcttac cacttactat
     4501 acaatgttca gttttgattt tgggcattct ttggcccgtt ttgctcccgt ctttatcaat
     4561 caaataacct cctgcctgaa caaaaactaa atttttatca ttgaagcttt caattagatt
     4621 tactgcatca gatataaaat ggggatctgt taaatagtca tctccatcaa gatttaatgc
     4681 ccacgagcct gtgctgtatt tatataagca agttctgtaa ttttctacac gtcctaatcg
     4741 cggattgttg cgaatgtatt taaaacgttt gtctgttatt tcttcaagaa tattttttgt
     4801 atcgtcagac gaactgtcgt cagatacaat tacttctata ttaggataat cttgagctaa
     4861 tgcactatca acagatctct taatatataa gggctgattg taggtaggta taagtatgga
     4921 tatgcgtaaa tccatggtta taagttctct atcgttgaac aaaaatgtga ataattttcg
     4981 ttagcatcac acattgtttc aaatcttttt cttgcgttgt ttcgtagttc tgcgtttgct
     5041 ttattttcat aaaaattagt aagcagtgat gctagctcat cagatttaat gtcttttgat
     5101 accaaatacc ccacattctc atcaacaatt tctgcaacac caccaacttt tgtcgctaaa
     5161 acaggaattc caaacgacaa tgcttccata atagaaacgg gaatgccttc tgtttcgcta
     5221 acattaacaa aaatatcaaa atgctgtttg gcatatagtt gcatcacaaa ctcgtgattt
     5281 tgttgtccca tccaatcgat cttaatatta tcaggacatt gcttcgattg ttcttttaaa
     5341 ttcttaagta atggtccgct gcctatatgt gtccatttaa cattaaacgg caaacacatt
     5401 acagcttcga gcagtaacga taagcgttta attttaatta tgtttgagca agtgagtaaa
     5461 tgcagtgtat tatcgtttga tgcagttgga ttcagaccat tgtcaaaaac cccaagacga
     5521 gatgtttcta tacgatttgg gcatagaaac ccatattttt ttaaatactc atgtccgtat
     5581 tcagatattg gagctgccat atctaaatag ggtaaaaccc attttctaaa aggaatgtat
     5641 ccgcctttaa cttcttcgta taaatcagat ccgtgaaatc tagcgatagc tttagtttta
     5701 aatttttgtt ttaaaaaggg gagcatgttt attgatttgt ctccccagta aaaataaagt
     5761 aaatcgttgc ttgaaggaag tgctttaaaa gatttgtaag cgctgcgaaa tacaagtagc
     5821 gaggtcataa aattccaaat gcgttttctg tttttatatg ctttttcttt aaaaaataat
     5881 ttacaggcaa aaaacaatgg agacgaattg aaaactcctg ctttaagcaa tgctatttta
     5941 tgtttaggat cttcattaag aagacttttc attacaacaa agtttgctgg aatcttgcgc
     6001 attattccgt tatatttatg taaaggaata actcttacaa cagaaaatgt tttactaagg
     6061 aaatgagact cgtcttgcaa aaaactttca ccatttccgt aaggccacga atcggtaaat
     6121 aaatataatg tgcgattcgt catttaaagt ttaatttttt tgttttaatg taagctatca
     6181 gcctcaaaaa cagatttttg aaaaacactc tgataattat aatatataag gcaaaggtaa
     6241 ataatacttg taatgtcagc gttaagtata aattgtttga tataaagcta gtgccgatta
     6301 ccagcagtgc tactgccaat cctataatta tacccggtaa tacctgcgta aattgttgtt
     6361 taccattgtt tcctacaaat ttgtatatgc ataatacaga acaaataaat gcaatccagc
     6421 atgacaagat gtatccgtat aacatagtat ttatgccata agtgaagcat gttaatatgg
     6481 atgttaaaat aagcacgcgt ttaaataatt ccatgcgaaa tgttatgtgc gatttcccca
     6541 tcgaattaag tgagtgtatg tttatttgat atataggagc aaataaattc gcggcgcata
     6601 ttagttgaaa aaaggggaca gaatcaagcc attttgttcc aaataataca aaaaacaaag
     6661 gctttgcact tgctatcaaa aacattgtga tagggattgt aactatagag gtgtttttaa
     6721 ccaattctcc aagtatgcgt ttaattctct cttgttgatg ctgaatcttt gcaaaaagat
     6781 tgtagctagt tcctaatatg gccagaaatg taaaattgac actatcgctg aatttatatc
     6841 cctgagtata atatcctact tgattaattg agtaaaattt acctattaaa aacgtgtata
     6901 tattattaaa aataactgta agtaaagagg taccaagcat gtgtgaacta aattggaaat
     6961 attcgc
//
LOCUS       DAOPID010000061         6934 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_14009_length_6934_cov_4.419538, whole genome shotgun sequence.
ACCESSION   DAOPID010000061 DAOPID010000000
VERSION     DAOPID010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 6934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6934
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14009_length_6934_cov_4.419538"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PK641_03250"
     CDS             <1..452
                     /locus_tag="PK641_03250"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HPG55402.1"
                     /translation="KKTDSTYTVTAMKETLDRSNLGLWIIGTEVNVERSMLMNGHLDG
                     HIVQGHVDQTARCIDVQENGGSWFFTFQYKSSEEMARRGYITVDKGSVTVNGVSLTVC
                     NPTEDTFQVSIIPYTYEHTNFHSIKIDSIVNIEFDIIGKYVSRMMGK"
     gene            521..1555
                     /gene="rlmN"
                     /locus_tag="PK641_03255"
     CDS             521..1555
                     /gene="rlmN"
                     /locus_tag="PK641_03255"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPG55403.1"
                     /translation="MADEKKHLLGKTLFELKEMAASLDMPTFAAKQIADWLYKKNITT
                     IDMMSNLSAANRTKLAENYTVGIKKPIKTSLSKDGTKKYLFDVGNNKYIESVFIPEKE
                     RATLCVSSQKGCQMNCLFCATGKQSFAGNLTSAEIINQVQSIDECDKLTNIVFMGMGE
                     PMNNINEVLKAIEILTSDYGYGWSPKRITLSTIGVLPALKTFLDSTQCNLAVSIHAPD
                     AFKRVQIIPGEKAYPIKDVLKLLKEYDWSHQRRVSFEYILFNQLNDTEEDALKLADLL
                     QGLDCRVNLIPFHQIPDVDLSPSKRDIMEKFQAILEKKHITATIRKSRGQDIDAACGM
                     LSTKKINQTL"
     gene            1552..2547
                     /locus_tag="PK641_03260"
     CDS             1552..2547
                     /locus_tag="PK641_03260"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HPG55404.1"
                     /translation="MRYKLLLFLSVLISFTACNYISLKPNSTGAANEILVVIDDSIKN
                     SAVGDSLFAILNQDVPGLNQSEPYFNISLTSTKNFASILKPARNIIIVSINNTYSRSK
                     ISHYKDQWSKPQSILKINGFDIADVANTISKYEDEILQYFIKAERERSISYQTRYSER
                     DAMKKVKDKFGFSIVLPKGMNRFKNEDNFMWIANSSREVNQNIVIYTYPYTENDAFTR
                     EKILHKRDSVMMLWIPGPSPDSYMGTEYRYNPPIARETSLENGNYAMEVRGLWTAIGD
                     IMGGPFVSISCLDEKNQQVVTAEAFVYAPNKYKRNIMRQLEAILYTINFETDGNK"
     gene            2534..3514
                     /gene="pdxA"
                     /locus_tag="PK641_03265"
                     /pseudo
     CDS             2534..3514
                     /gene="pdxA"
                     /locus_tag="PK641_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289667.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
     gene            3535..4743
                     /locus_tag="PK641_03270"
     CDS             3535..4743
                     /locus_tag="PK641_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPG55405.1"
                     /translation="MESSQLQTIKQRFDIVGNTPDLDQAIRVAVQVAPTDLPVLITGE
                     SGVGKENFPKIIHQYSVRKHEKYIAVNCGAIPEGTMDSELFGHEKGSFTGALTDRKGY
                     FEVADGGTIFLDEVGELPLPTQARLLRVLQDGEFFRVGSSDLKKTNVRVVAATNINIQ
                     QAIREGKFRGDLYYRLNAVPIQIPPLRERKIDIMPLFTKFANDFAETYRRPPVKLTAE
                     AQKMLVNYYWQGNIRQLKNVAEQISAVETNPEIEATTLAHYLPDESQSRLPLIVSNEK
                     NEKTFNNEREILYQVLFDMKKDMDELKKSVSKLMTESNSIVPKEPKPVQHHEQTVISY
                     TPTDIRNEDYEDAQEYDESPLAVDDMEKELIKKALEKHAGKRKYAAMDLKISERTLYR
                     KIKEYGLDNI"
     gene            4761..5273
                     /locus_tag="PK641_03275"
     CDS             4761..5273
                     /locus_tag="PK641_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HPG55406.1"
                     /translation="MKYFKPFEIAIVGLILMSSCSISYKFNGTVIDYDKIKSISIVDF
                     TNQAAMVYAPLTAKFNEELKDIYTRQTRLNLISRNADLELDGEITGYDITPMSISTNS
                     LASETRLTIKINVRYINNTNHEEDFEKVYSAYRGFDSKRMLTDVQDELIEEITKEICE
                     NIYNDTVANW"
     gene            5276..>5794
                     /locus_tag="PK641_03280"
     CDS             5276..>5794
                     /locus_tag="PK641_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55407.1"
                     /translation="MTSEQLIYYFKHPDELDKQTVSDLQYLVNEYPYFQSIQLLYLKN
                     LQNIGDLQYHAMLKKTALYAADRSHLQDLMNVLPQIKSKTEEKPISKSETILTETTEY
                     KLVESESVDNQSVPALQHQDLIDKFISDNPRIHPVGQIESNNIVIPDIQEDTDVSSSI
                     FTESLAKIYIKQK"
     assembly_gap    5795..5804
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5929..6267
                     /gene="secG"
                     /locus_tag="PK641_03285"
     CDS             5929..6267
                     /gene="secG"
                     /locus_tag="PK641_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005657777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPG55408.1"
                     /translation="MYNVLVSLAVVISILLTIIVSVQKPKGGGLAANFSASNNILGVR
                     KTTDFLEKATWSLVIILVVLSVASAAFISKGVSAEEAQSSALKNIIEQTATPNANQTP
                     IMPTSTPAAE"
     gene            6587..6856
                     /locus_tag="PK641_03290"
     CDS             6587..6856
                     /locus_tag="PK641_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPG55409.1"
                     /translation="MNIKPLADRVLVKPNAAEEKTASGIILPDSAKEKPLKGKVVAIG
                     NGTKDEEMIVKVGDNVLYGKYAGTEIELDSEKYLIMRQSDILAII"
BASE COUNT         2620 a         1144 c         1205 g         1955 t
ORIGIN      
        1 ttaaaaagac cgatagcaca tatacagtaa ctgctatgaa agaaacctta gatcgtagta
       61 atctgggttt atggattatc ggaactgaag ttaatgttga acgcagtatg ctcatgaacg
      121 gacatttaga cggacatata gtacaaggtc atgttgatca aacagcaaga tgtattgatg
      181 ttcaggaaaa tggcggcagt tggttcttca cttttcaata taagagttca gaagaaatgg
      241 caagacgtgg atacataact gttgataaag gttctgtaac tgtaaatggt gtcagtttga
      301 cagtatgcaa ccctactgaa gacacttttc aggtatcaat aataccctat acctacgagc
      361 acacaaattt tcatagcata aaaattgata gtatcgtaaa tatagaattt gacatcatcg
      421 ggaaatacgt aagccgtatg atgggcaaat aaacaaaatt tgcactatct ttgcattgcg
      481 taattttgca gtattctaca taatacaaat atctataagt atggcggatg aaaaaaaaca
      541 cctcttgggt aaaactcttt ttgaattaaa agagatggca gcttctttgg atatgcctac
      601 ttttgccgcc aaacaaattg cagattggtt atataaaaag aatattacca ccattgatat
      661 gatgagtaat ctttctgctg caaacagaac caaactggca gaaaattata ctgttggtat
      721 aaaaaagcct ataaaaacaa gcttatcaaa agatggtaca aagaaatact tatttgacgt
      781 tggaaacaat aagtatatag agagcgtatt tatacccgaa aaagaacgcg caaccttgtg
      841 tgtttcatca caaaagggat gtcagatgaa ttgtttgttt tgtgcaacag gaaagcaaag
      901 ttttgccggt aatttaacat ccgcagaaat aattaatcag gttcaaagta ttgacgaatg
      961 cgataaattg accaatatag tttttatggg tatgggtgaa cccatgaata atataaatga
     1021 ggttttgaaa gccatagaaa ttctaacttc cgattacgga tacggatgga gtccaaaacg
     1081 cattacatta tctactatag gagttttacc ggctctgaaa acattcttag attcaactca
     1141 atgtaacctt gctgttagca tacatgctcc tgatgccttt aaacgtgtac aaataattcc
     1201 gggtgaaaaa gcatacccca ttaaagacgt gttgaaactt ttaaaagagt acgattggag
     1261 tcatcaaaga cgtgtatctt tcgagtatat attatttaat caattaaatg acacggaaga
     1321 agatgcacta aaactagcag atttgttaca aggattggat tgccgagtta atttaatccc
     1381 attccaccaa atacctgacg ttgatttgag cccttcaaaa cgcgatatta tggagaaatt
     1441 tcaagctata ttagaaaaaa aacatatcac tgcaactatt cgtaaatcgc gtggacaaga
     1501 tattgatgct gcctgtggta tgttatcaac aaaaaaaata aatcaaacat tatgagatac
     1561 aagttattac tttttttgtc agttttaatt tcttttacag catgtaacta cattagttta
     1621 aaacctaatt caacaggagc tgcaaatgaa attctagtcg taattgatga ttcaattaag
     1681 aattcggcag ttggtgattc tttgtttgca attcttaatc aagacgttcc cggacttaat
     1741 cagtcagagc catattttaa tatatcactc acaagcacaa aaaactttgc aagtatttta
     1801 aaacctgcgc gaaacataat tattgtaagt ataaataata cctattcgcg tagtaaaata
     1861 agtcattaca aagatcaatg gtcaaagcca caatctattc taaaaattaa tggatttgac
     1921 attgctgacg tcgcaaatac aatatcaaaa tacgaagacg agatcttgca atactttata
     1981 aaagcagaaa gagaacgttc aatatcttac caaacacgtt attccgaaag agatgctatg
     2041 aaaaaagtaa aagacaagtt cggattttca atagttttgc ctaaaggaat gaatcgcttt
     2101 aaaaatgaag ataactttat gtggattgcc aattcaagta gagaggtaaa tcaaaacatt
     2161 gtgatttata catatccata taccgaaaat gatgctttta ctcgcgaaaa aatattgcat
     2221 aaaagagatt ctgtaatgat gctatggata cccggaccat ctcccgactc atacatggga
     2281 actgagtata gatataatcc ccctattgcc cgtgaaacat cacttgaaaa tggtaattat
     2341 gcaatggaag tacgcggttt atggacagcc ataggcgaca ttatgggtgg accttttgtc
     2401 agtataagtt gtttagacga aaaaaatcaa caagtagtta cagcagaagc attcgtatat
     2461 gctccaaata aatacaaacg taatattatg aggcaattag aagccatttt atatacaata
     2521 aattttgaaa cagatggaaa caaataaaat aaaattagga ataactcaag gtgatataaa
     2581 tggtatcggt tacgaagtaa tcctaaaaac gcttgaagat aacagaatct tagatttatg
     2641 tattccaatc gtatatggat cacctaaagt tgcagcttat caccgcaaaa cctttgattt
     2701 aaatatcaat cttaatctaa taaataatgc aagtgaagcc agcgataaac gtgctaacat
     2761 aattaattgc tgcgacgatg aaataaaggt tgatttaggt ttatctactc caatggcagg
     2821 tgccgctgca tttgctgcat tagaaaaagc atcacaagat ttaaaagaag gtttgattga
     2881 tgcaattgta accgcaccaa tcaacaaaaa aaacatacaa tctcaaagtt ttcaatttcc
     2941 cggtcatacc gaatatttag aagaaacatt tggaaaacga ggagatgctt taatgctact
     3001 tacgaacgat aaaatgcgtg tagctgttgt aaccggacat attccattag ctaaagttgc
     3061 acaaacaata acaagcgatt taattcttgc aaaacttcgc atattcaaca aatcgcttaa
     3121 acaagatttc tctataaata gaccccgcat tgccgtactt ggactaaatc cacattcagg
     3181 tgatgaagga gtaattggaa ctgaagaaaa agaaattata gctcctgcct taaaaacggc
     3241 tatggatgaa ggtattattt gcataggacc acaagctgca gacgggttct tcggctctga
     3301 taattatcta aaatatgatg gaatactagc aatgtatcac gaccaaggtc tgattccatt
     3361 caaagcaatg gctatggatt caggagttaa ttttacagca ggtttaaatg tagttagaac
     3421 atcacctgac catggcacgg catatgacat tgccggacag aacttggcat cagaaaattc
     3481 attccgtcaa gctctttaca tggctgttga tattatagca aatcgtagga aatgatagaa
     3541 tcaagtcaat tacaaacaat taaacaaagg ttcgatatag tcggcaatac tcctgacctc
     3601 gaccaagcaa ttcgtgtagc cgtacaagtt gctcctaccg atttacctgt attaattaca
     3661 ggcgaaagcg gagtcggaaa ggaaaatttt cctaaaataa tacatcaata cagtgtccgt
     3721 aaacacgaaa aatatattgc agttaactgc ggtgcaatac ccgaaggcac aatggattct
     3781 gaactattcg gacacgaaaa aggttctttt acaggagctc taacagaccg aaaaggttat
     3841 tttgaggtag ctgacggcgg aactatattt ttggacgaag taggagaact tccccttccc
     3901 actcaagctc gattattaag agtattacaa gatggcgaat tctttagagt cggttcttcc
     3961 gatcttaaaa aaacaaatgt aagagttgtt gcagctacaa atataaatat tcaacaagca
     4021 attagagaag gtaaatttag aggagatttg tattatcgtt taaatgctgt gccaatacaa
     4081 ataccgccac tgcgcgaacg taaaattgac ataatgccat tatttacaaa attcgccaat
     4141 gattttgcag agacatacag aagacctcct gttaaattga ctgccgaagc tcaaaaaatg
     4201 ttagtcaact actattggca aggtaatatt cgacaactta aaaacgttgc cgaacaaata
     4261 tcagctgtag aaacaaatcc tgaaatagaa gctacaacat tagcacatta tttacctgat
     4321 gaatcacaaa gtcgcttacc tcttatagtt tcaaatgaga agaacgaaaa aacatttaat
     4381 aacgagcgcg aaatcttata tcaagtcctt tttgatatga agaaggatat ggatgaactt
     4441 aagaaatcgg ttagtaaatt aatgacagag agcaattcta ttgtccctaa agagcctaaa
     4501 ccggttcaac atcatgagca aacagtaatt tcatatacac ctaccgacat tcgaaacgaa
     4561 gattatgaag atgctcaaga gtatgacgaa tctccattgg cggtagatga tatggaaaaa
     4621 gaacttatca aaaaagctct cgaaaaacat gccgggaaaa gaaaatatgc tgccatggat
     4681 ttaaagattt cagaacgcac tttgtacaga aaaattaaag aatacggatt agataacata
     4741 taatatcaca cattatatag atgaaatatt ttaaaccatt tgaaatagca atcgtaggct
     4801 tgattctaat gtcatcgtgc agtatatcat ataaatttaa tggtactgtg attgactatg
     4861 ataaaattaa atctatttct atagttgatt ttacaaatca agcagctatg gtatatgccc
     4921 cactgactgc aaaattcaac gaagaattaa aagacatata tacacgtcaa acgcgtttaa
     4981 acttgatttc gcgtaacgca gatctcgaat tagatggaga aattaccgga tatgatatta
     5041 ctccgatgtc tatatcaaca aattcattgg catcagaaac gcgattaact ataaaaatca
     5101 acgttcgcta tatcaataat acaaatcacg aagaagattt tgaaaaagtt tattcagcat
     5161 accgcggttt cgacagtaaa agaatgttaa ctgatgttca agatgaatta attgaagaga
     5221 ttacaaaaga aatatgcgaa aatatttata atgacactgt tgctaattgg taataatgac
     5281 aagcgaacaa cttatatatt atttcaaaca tcctgatgaa ttggacaaac aaaccgtttc
     5341 cgatctacag tatcttgtaa atgagtatcc gtatttccaa tcaatacaat tattatactt
     5401 aaaaaactta caaaatatag gagacttaca atatcatgca atgctaaaga aaacagcttt
     5461 atatgcagct gacagatcgc atttgcaaga tttaatgaat gttcttcctc aaattaaatc
     5521 taaaacagaa gaaaagccta tttcgaaaag cgaaaccatc ttgacagaaa ccacagaata
     5581 taaattagtt gaatcagaaa gcgttgacaa tcaatctgta ccggcacttc agcatcaaga
     5641 tttaatagat aaatttattt cggataatcc acgtatacac cctgttggac aaatagaatc
     5701 aaataacata gttattcctg acatacaaga agacacagat gttagttcaa gtatatttac
     5761 agaaagtctg gctaaaattt atataaaaca gaaannnnnn nnnnagaaac aatatacaaa
     5821 agcactacga atttttgaaa aattaaattt gaaatatcca gaaaaaagta gttactttgc
     5881 agaccaaatt cgatttttag ataaaataat tcaaaataac aaataattat gtacaacgta
     5941 ctcgtatcat tagctgtcgt tatttctatc ttattaacca ttattgtatc agtacaaaag
     6001 ccaaaaggag gcggtttggc agcaaatttt tcggcatcaa acaatatatt aggtgttcgt
     6061 aaaacaactg atttcttaga aaaagcaaca tggtcattag ttataatact agtagtttta
     6121 agcgtagcaa gtgctgcttt tatttctaaa ggagtatctg cagaagaagc acaatcatct
     6181 gctttgaaaa acattataga gcaaacagca acaccaaatg ctaatcaaac tccaataatg
     6241 cctacatcaa ctccggctgc agaataatcg aagctacaga ttacatataa atagtcataa
     6301 taagtcgttt tgtcataaca aagcgactta ttttttttgg cacgatatat gcaatgggtg
     6361 gttatgggtg aagggcgtgg aatgtgtagt gtgtaatgtg tagggttatg ggtgaagggt
     6421 taagggcgtt aaacattaaa cgattacaca ttaaacaatt aaacgattac acattaaaca
     6481 attacacgat tacacgatta aacgattaca caattaaacg attaaacaat tacacaatta
     6541 aacgattaac taattaaaca tttaaacaat taataattac acaactatga acattaaacc
     6601 attagcagac agggttttag ttaaaccaaa tgctgcagaa gaaaaaacag caagcggaat
     6661 cattcttccg gattctgcaa aggaaaaacc tttaaaaggc aaagttgttg caataggcaa
     6721 cggaacaaaa gatgaagaga tgatagtaaa agttggtgat aacgtactat acggcaaata
     6781 tgccggtaca gaaatcgaat tggattctga aaaatatctt atcatgcgcc aaagtgacat
     6841 tttggcaatt atctaatgac aattttaaat tttataaatc atggcaaaag aaattaaatt
     6901 tgacatcgac gcacgcgacc gcttgaaagg atcg
//
LOCUS       DAOPID010000062         6878 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_14148_length_6878_cov_3.801114, whole genome shotgun sequence.
ACCESSION   DAOPID010000062 DAOPID010000000
VERSION     DAOPID010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 6878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6878
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14148_length_6878_cov_3.801114"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PK641_03295"
     CDS             <1..227
                     /locus_tag="PK641_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55410.1"
                     /translation="YSNSYAKVNGAYYNEFAITMGIGFPLFSSLTTLNLLLEYGQRGS
                     VSTTKLLEQYFKIGLNVSLNERWFVKRKFN"
     gene            256..1614
                     /locus_tag="PK641_03300"
     CDS             256..1614
                     /locus_tag="PK641_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55411.1"
                     /translation="MKNFKYIFMVGALISSISAYSAKGVDDGSRFGTGEDSIRCIENL
                     SLYGSYYKIKDYESAYNSWKIVYDECPQAGGRTLYSQGAFLIATKMSKEKDAVKKQEW
                     FKMLMECYDKRIQYFGDDKNYPGPWIRGRQAIDYINYSGDVDLITKALPWLKTAVDYM
                     GEKCDADVLNAYFQMLEKQYESNKDEYRTNFINEYLRLGSILDGRIAKADKYVPNYQL
                     VRNNINQMFTNSGAADCATLESVFASKVETSKENVDELGTIITLFSKAGCKESDVYFK
                     ASLYAHKLNPTAETAAGCGYQAAKKKEYSNAVRFFNEAIKLADNDSVKYEYQYIIAAL
                     EMQMDHYTEARAAARRAIELDPSNGAPYILIAQMYADNSHNPYTDDKILAKTVYWAAV
                     DKLEKAKSVDPSIADKAQQLINSYRKFYPSKEDVFFKPELNVGELFLIGGWIGETVRC
                     RD"
     gene            1627..2232
                     /gene="lptC"
                     /locus_tag="PK641_03305"
     CDS             1627..2232
                     /gene="lptC"
                     /locus_tag="PK641_03305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HPG55412.1"
                     /translation="MKKILIKYLNTYITVAFLATVMFVFISCSSRVKSTEELPNSRDS
                     VALIHAWDVSTVISDSGITRYRLETPEWLVFDKAEEPYWDFPHGIHLEKFDENLNVYA
                     NVDADTAIYYNHKEMWILSGNVKAMNLDGEKFESEKLFVEQRNDRIYTDRFVTITQKE
                     RIINGIGLESNQKLTKYTILEPQGIIPIEEAPDSVSTSENQ"
     gene            2238..3491
                     /locus_tag="PK641_03310"
     CDS             2238..3491
                     /locus_tag="PK641_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008626995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPG55413.1"
                     /translation="MSSTIVIIIISLLFSAFFSGMEIAFVSSNKLRFEMDKQRKTLTS
                     SILNIFYTRPDRYISTILVGNNIALVIYGIQMANLLHPFLLPLGSDALIALIQTLLST
                     FLILITGEFIPKILFKINPNFWMNTFAAPLLLIYVILYPISVFMTLLSKAIIRLFGIK
                     VAERSGRLLGRVDLNFLLHESVDNHNNKKDEINNEVKIFQNALDFSKVKLRDCMVPRT
                     EIEAVDSDTVLEELTQRFVSSGCSKILVYKESIDNIIGYIHSIEVYKHPKKWQDKIIL
                     VPIVPETMAANKLMNLLLLKKKSLAVVVDEFGGTAGIVTLEDIVEEIFGEIEDEHDTQ
                     EYVEKRINDHELVLSGRLEIDYINEQFGLQLPTSDEYLTIAGLILHIYQGFPQTNDEI
                     KSGNYLFKVLKVTHNKIELVRLIIQ"
     gene            3580..5664
                     /locus_tag="PK641_03315"
     CDS             3580..5664
                     /locus_tag="PK641_03315"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HPG55414.1"
                     /translation="MATLQKIRQRGVFLTVIIGLALLAFIIGGIDFNSIFGKSRTVVA
                     KVDGDEIDIREYEKRIDEMTAFYKIESGQNSLSDQYVNQVRNSVWNSWLTEKILGEQC
                     KKLGIVVSDDELADRLTGSNPHPMLNQLRAFYNPETGGFDKNAFIQFISQIGTESNAE
                     NEDYVKYWSFIQRTIREQILEEKYNTLVGASFQINDIDAKYAYEAKKAFNIEFVQTPY
                     STLPDSTFSVSDNEIKDRYNKEKNNFYKEDESREIGMITFAIQPSQKDFEDIKTWIEG
                     LQNEFATSADYIALANQNSDVSYTGIPVSKSEIDPDLLDFAFNGKAGDFYAPQLKGRT
                     YKMARLVETGIMASDSVKARHILIMESSKEKTQALADSLTAALKSGSDFATTAKTFSK
                     AGTAQNGGELGWFRDGDLDKDFSAVCIKANVNEIFQFPMGQAIQIIQVTEKTKPVSKV
                     KICVLARDVEASSSTYGTIYNQASQYVAKNTNQADFEKNADPSKGEFLRTYTVTKNDN
                     IIADLKDSRQIIRWAFEKDLGDVAENVFECGDKFAVVTLKSITESGNQSIDVVKDQIK
                     SEIIKEKKSEKIVADMKTRLGSSQDLSVLGAPQVATNVSMSSNYIQNLGSEPKMLAEL
                     ASTVNQKDLKFFQGANSTIAAKVTEVIPSQTEFDAKVEINQLSMRRPYQYMLFESLKS
                     SSDITDNRINFY"
     gene            5837..>6878
                     /locus_tag="PK641_03320"
     CDS             5837..>6878
                     /locus_tag="PK641_03320"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HPG55415.1"
                     /translation="MHKRLTISLLLLANLLYVFAVPAFPDLILLNQINGNDLAIRIGG
                     DEYFHYTLTSDGFLIAQNAMGIYEYAEYKNSEMVLSGVAAKDPAVRPKDHEKHLHTFS
                     VEDGVKQSTKMRLAYDIDNEPIILTKKSQDTIHIPVILVSFSDIKFVYDKQDVDSLLN
                     AKIMDKTIPTGSVQTYLSHSTNKKLNVKFDVYGPYNIPGTCAYYGANQPSCTGLCDIN
                     PRGMVIDACNAADADIVFSDYATDGVKVDQVLVMYAGYNEAENATYTDFIWPHKWQLA
                     NKNTILDGVIIYDYTCVSEMRGDYSKHEDKLCGIGTIVHEFSHTLGLPDLYPTNGGNY
                     FTVGYWDVMCYGV"
BASE COUNT         2432 a          965 c         1253 g         2228 t
ORIGIN      
        1 attattcaaa ttcatacgca aaagttaatg gtgcatacta taacgaattc gcaattacta
       61 tgggtatcgg tttccctttg tttagttcac ttacaacatt aaatttatta ttagaatatg
      121 gccaacgtgg ttctgtatca acaacaaaat tgcttgaaca gtatttcaaa attggattga
      181 atgtgtctct gaacgaacgt tggtttgtta aaaggaaatt taattaatac aatatacaac
      241 tttaacatta cggaaatgaa aaactttaag tacattttta tggtaggagc gttaataagc
      301 tctatatctg cgtatagcgc aaaaggtgtc gatgatggaa gtcgctttgg aacaggtgaa
      361 gatagtatcc gttgtatcga aaatctaagt ctttatggca gttattataa aattaaagat
      421 tatgaatctg cgtataacag ctggaagatt gtttatgacg aatgtcctca agcaggtggt
      481 cgtactcttt atagtcaagg tgcattttta attgctacta aaatgagcaa agaaaaagat
      541 gcagttaaaa aacaagaatg gtttaaaatg ttgatggaat gctatgataa gcgtattcaa
      601 tattttggag acgataaaaa ttatcccgga ccatggatac gtggacgtca agctattgat
      661 tatataaact attccggtga tgtagattta ataacaaaag cattaccatg gttaaaaaca
      721 gcagttgatt atatgggcga aaaatgtgat gctgacgttt tgaatgctta tttccaaatg
      781 ttggaaaagc aatatgaatc aaataaagat gaatatagaa caaattttat taacgaatat
      841 ttaagattgg gttcaatatt agatggtaga attgcaaaag cagataaata tgttccaaac
      901 tatcaattag tgcgcaataa tattaatcaa atgtttacca atagtggtgc tgccgattgt
      961 gctacacttg aaagcgtatt tgcatcaaaa gttgaaacgt ctaaagaaaa tgttgatgaa
     1021 ctaggaacta ttataacttt gttctcaaag gccggatgta aagaatctga tgtttatttt
     1081 aaagcatctt tatatgctca taaattgaat cctacagcag aaacagctgc aggatgcggt
     1141 tatcaagcag caaagaaaaa agaatattca aatgcagttc gtttctttaa cgaagcaatt
     1201 aaattggctg acaatgattc tgttaaatat gaatatcagt atataatagc ggctctggaa
     1261 atgcaaatgg atcattacac agaagctcgt gcagctgcaa gacgtgcaat agaacttgat
     1321 ccatctaatg gcgctccata tatcttaata gctcaaatgt atgcagacaa tagtcataat
     1381 ccatatactg atgacaagat tttagcaaaa actgtttatt gggcggctgt tgataaatta
     1441 gagaaagcaa aatcagtcga tccttcaatt gcagacaaag cacaacaact tataaatagc
     1501 tatcgcaaat tttacccttc aaaagaagat gtattcttta aaccggaatt gaatgttgga
     1561 gaattgttct taattggtgg atggataggg gagacagttc gttgtaggga ctaagttttt
     1621 tctgatttga agaaaatatt aattaaatat cttaatactt acataacggt cgctttttta
     1681 gcaaccgtta tgtttgtgtt tatttcctgt tcatctcgag ttaaatccac ggaagaatta
     1741 cccaattcac gtgattctgt cgcactaatt catgcatggg atgtttcaac tgttatatca
     1801 gattctggaa ttaccagata tcgtcttgaa acacctgaat ggttggtatt tgacaaagca
     1861 gaggaaccat attgggattt tccacatgga atacatttag aaaaatttga tgaaaattta
     1921 aatgtttatg caaatgtaga tgcggataca gcaatatatt acaatcataa agaaatgtgg
     1981 attttgagtg ggaacgtaaa ggcaatgaat cttgatggag aaaaatttga gtccgaaaaa
     2041 ttattcgtgg agcaaagaaa tgatcgtatt tacacagaca gatttgtaac cataacacaa
     2101 aaagaacgta taattaatgg aattggttta gaatccaatc aaaagttaac aaaatatact
     2161 atattggaac cgcaaggtat aatacctata gaagaagcac ctgattctgt ttcaacatct
     2221 gaaaatcagt aattaatatg agttctacta ttgtcataat cataatttcg ttgttatttt
     2281 cagctttttt ttcaggcatg gaaatagcgt ttgtttcatc taacaaactt cgatttgaaa
     2341 tggataaaca acgtaaaacg ctaacatctt ccatacttaa tatattttat acaagaccgg
     2401 atagatacat ctctacaatt ttagttggaa acaacatagc attagtcatt tatggtatac
     2461 aaatggcaaa tttattgcat ccgtttttac taccattagg cagtgatgca ttaatagcat
     2521 tgattcagac actgctttcg acatttctta ttcttataac aggagagttt attccgaaaa
     2581 tattgtttaa aatcaatcct aatttttgga tgaacacatt tgcagcgcct ttattattaa
     2641 tatatgtgat tctatatccg atatctgttt ttatgacttt attgtcaaaa gctataattc
     2701 gtttgtttgg aattaaagtt gcagagcgct cagggcgttt gcttggaagg gtcgacttaa
     2761 attttctttt gcatgaatct gttgataatc acaataataa gaaagatgaa attaataatg
     2821 aagttaaaat ctttcaaaat gcccttgact tctcaaaagt aaaactacgc gactgtatgg
     2881 ttccaagaac ggaaattgaa gcagtagatt ccgatactgt attagaagag ctaacgcagc
     2941 gttttgtatc gtcaggttgt tcaaaaattt tagtctataa agagagtata gataatatca
     3001 taggttatat acattctatt gaggtgtata aacatcctaa aaaatggcaa gataagatta
     3061 ttttagtacc gattgttccg gaaacaatgg ctgcaaataa attgatgaat ttactgctat
     3121 tgaaaaagaa aagtctagca gttgtggtag acgaatttgg cggtactgca ggtattgtaa
     3181 ctttggagga tattgtagaa gagatttttg gcgaaattga agatgaacat gatacgcaag
     3241 aatacgtaga aaaacgtatt aatgatcatg aactggtact ttcaggaaga ttggaaatag
     3301 attatatcaa tgagcaattc ggattgcaat tgcctacttc tgatgaatac ttaacgatag
     3361 ccggtttaat attacatatt tatcaaggat tcccacaaac gaacgatgaa attaagtctg
     3421 ggaattattt gtttaaagtt ttaaaagtaa ctcacaataa aattgaatta gtacgattga
     3481 taatacaata aaaatgaagt gcattattta aaaaatttgt atcttcgcaa cctaattgtt
     3541 cagcaaattt aaaaacaata atattatttt tcatataaaa tggcaacatt acaaaaaatc
     3601 agacaaagag gagtcttttt gacagttatt atcggtcttg cattattggc ctttatcata
     3661 ggaggcattg attttaattc tatatttggt aaatccagaa cagtggttgc aaaagtagat
     3721 ggcgatgaaa tcgatatacg cgaatatgaa aagcgtattg acgaaatgac agctttctat
     3781 aagattgaat caggacaaaa tagtttgtca gaccaatatg ttaatcaagt acgtaattct
     3841 gtatggaatt cttggttaac agagaaaata ttgggtgaac aatgcaagaa attaggtatt
     3901 gttgtttctg atgatgaatt agctgatcgc ttaacaggtt ctaatccaca tccaatgttg
     3961 aatcagttac gtgcatttta taatccggaa acaggtgggt ttgataaaaa tgcattcatt
     4021 cagtttatat ctcagatagg tactgaatca aatgcagaaa atgaagatta tgtaaaatat
     4081 tggtcgttta tacaaagaac tattcgcgaa cagattttgg aggaaaaata taacacatta
     4141 gttggtgcat cattccaaat taatgatata gatgcaaaat atgcgtatga agcaaaaaaa
     4201 gcatttaata tcgaatttgt tcaaactccg tattctacac ttcccgattc aactttttct
     4261 gtaagtgata atgaaatcaa agatcgttat aataaagaaa aaaataattt ctataaagaa
     4321 gatgagagcc gcgaaatcgg aatgattaca tttgcaatac aaccttctca aaaagatttt
     4381 gaagatatta aaacctggat tgaaggtttg caaaatgaat ttgcaacttc tgcagattat
     4441 atagcacttg caaatcaaaa ttcagatgtt agttatacag gaattcctgt aagcaaatca
     4501 gaaattgacc ctgatttact tgattttgcg ttcaatggaa aagcaggtga tttttatgca
     4561 ccacaattaa aaggccgtac ttacaaaatg gcacgtttag ttgaaaccgg tataatggca
     4621 tctgattcag ttaaagcgcg ccatatttta attatggagt cttcaaaaga aaaaactcaa
     4681 gcacttgcag atagtttaac tgcagcgctt aaatcaggtt ctgattttgc aacaacagct
     4741 aaaacatttt caaaagcagg aactgctcaa aatggtggcg aattaggatg gttccgtgat
     4801 ggtgatttgg acaaagattt cagtgcagtt tgtattaagg caaacgtaaa tgaaatattc
     4861 caattcccta tgggacaagc aatccagatt attcaagtaa cagagaaaac taaaccggtt
     4921 tctaaggtta agatttgcgt tttagctcgt gatgttgaag ctagttcaag tacatatgga
     4981 actatatata atcaagcaag tcaatatgtt gctaaaaaca caaatcaagc tgattttgag
     5041 aaaaatgctg atccttcaaa aggtgaattt ttacgtacat atacagttac aaagaatgat
     5101 aatattatag ctgatttaaa agattcacgt caaataatcc gttgggcgtt tgaaaaagat
     5161 cttggcgatg tagctgaaaa tgtttttgaa tgtggcgata aatttgctgt agttacatta
     5221 aaatcaatta ctgaatcagg aaatcaatct attgatgttg taaaagatca aattaaatca
     5281 gaaataatta aagagaaaaa atcggagaaa atcgttgcag atatgaaaac ccgtttaggc
     5341 tcttctcaag atctatctgt tttgggcgct cctcaggtag caacgaatgt ttcaatgtct
     5401 tctaattata ttcaaaattt aggttctgag cctaagatgt tagcagaatt ggcatctaca
     5461 gtaaatcaaa aagatcttaa attcttccaa ggtgcaaatt ctacaattgc tgctaaagta
     5521 acagaagtaa taccaagtca aacagaattt gatgccaaag tcgaaattaa ccagttgtca
     5581 atgcgtcgtc catatcaata catgttattt gaatctttaa aatcaagttc agacattact
     5641 gataatcgta ttaacttcta ttgattttat tgtaaatcac atattatcac ttaaatataa
     5701 taaccgacta agagatttta gtcggttatt ttttcattac atattatgaa ttattgtcat
     5761 aaataatgct taaccggcta atgtgctttc gttttttttt gcttactttg gtgtgtgata
     5821 caaaaatatt tttgttatgc ataaacgatt gactatcagc ttattattat tagctaattt
     5881 actatatgta ttcgcagtac ctgctttccc cgatttaata ttattaaatc aaattaatgg
     5941 taacgattta gctattagaa ttgggggtga tgaatatttt cattatacct taacttctga
     6001 tggctttttg attgcacaaa atgctatggg aatttatgaa tatgctgaat ataaaaattc
     6061 tgagatggta ttaagtggtg ttgctgctaa ggatcctgca gttaggccta aagatcatga
     6121 aaagcaccta cacacttttt cagttgaaga tggtgttaag caatcgacta aaatgcgctt
     6181 agcttatgat attgataatg aaccaatcat tttgacaaaa aaatctcaag atacaataca
     6241 catcccggtt atattggtat catttagtga tataaaattt gtatatgata agcaagatgt
     6301 tgattctttg cttaatgcta agataatgga taaaacaatt ccaacaggtt cagtacaaac
     6361 ttatttgtct cattcaacga ataaaaaatt aaatgtaaaa tttgatgtat atggtccgta
     6421 taacataccc ggtacttgtg cttattatgg cgcaaaccaa ccatcttgta caggactatg
     6481 cgatattaat ccgcgtggta tggttattga tgcttgtaat gctgctgatg ctgatattgt
     6541 ttttagtgac tatgctacag atggtgttaa ggtagatcaa gtgttagtta tgtatgccgg
     6601 atataatgaa gctgagaatg caacatacac tgattttatt tggcctcaca aatggcaatt
     6661 agctaataag aatactatac ttgatggtgt aataatatac gattatacat gtgtatcaga
     6721 aatgagaggt gattatagca agcatgaaga taaactttgt ggaataggaa ctatagtaca
     6781 cgaattttct catacattgg gtttgccgga tttgtatcca acaaatggtg gcaactattt
     6841 tactgtaggt tattgggatg ttatgtgtta tggagttt
//
LOCUS       DAOPID010000063         6865 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_14182_length_6865_cov_4.857709, whole genome shotgun sequence.
ACCESSION   DAOPID010000063 DAOPID010000000
VERSION     DAOPID010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 6865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6865
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14182_length_6865_cov_4.857709"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PK641_03325"
     CDS             <1..383
                     /locus_tag="PK641_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPG55416.1"
                     /translation="NNITSGGIVEIGISANPVSHSYPVRIKAANSNHRILPGMICNVY
                     ISDKNTKSGFVVPTNAILIANDNSKFVWLIENGKAVRRSIKISGYSGVGVLVSEGLNT
                     NDLVIVEGYQKVSEGMLVTAQTEQ"
     gene            393..3515
                     /locus_tag="PK641_03330"
     CDS             393..3515
                     /locus_tag="PK641_03330"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPG55417.1"
                     /translation="MKKGIISASITYRRIIHVAVLLLFAFGIYALSNMNKDEFPSFTL
                     RQGIIAGIYPGASVEEVENQLTKPLEQLLFSMPEINRENTYSISKNGIAYVYVDLDLS
                     VKDKDETWSKIRHKLNEQKSLTFPAGVLGFVVIDDFGNNSSMLIAIESNDKTYKELHS
                     YTNELSKRLRQIPEMGNIKILGERTEEIAVKIDQSKLASYGLNPQTLLLNYASRSLLL
                     PGGNYQALDFSMPVHINQSIASEKEIEDQIIYAFADGSSIRLKDIATVERRYQDLKND
                     VNFNGKNAIVVSAEMRKGNNIVAFGDEVNKILDDFSKITPQSVKVYRITDQPTVVDKS
                     VSSFLRDLIISMIVVIVVMLMLFPLRTALVASSGLPICTAVSIGLMYLFNIELNTVTL
                     AALIVVLGMVVDDSIINIDGYIDKLQKGYKPFEAAVTSAKELFMPMLISTAAISAMFF
                     PMKLIIKGPLGDFIQLFPWTILFSLASSLIYAMLVIPALEIKFIPTATAQRKPNLFER
                     GQAYFFNLIQDGYEKLQNTCFRHPVITITVGVASTLLGIVMFLQLNIQMMPMAERDCF
                     AIEIRLPEGSDISETKVVSDSLQNLLLQDKRIKSVTAFIGEPAPRFHITYAPSMPANN
                     LAQLIVNTQSVDATEELLPLLDKKYSYYFPKAHIRLRQLDYQAVTNPIEIHVLGDDDK
                     KIAEIADKIKSYMLTLNNELRWVHSSNDEYCPSVNINLKPEESARLGISQSFLALNLA
                     GTFNGLPLSSIWEDDIKIPINLYYADFNADSSYNNIGNQLIPTAIPNTWVPLRQVADA
                     SPEWHSSQIIRRFDRNCITVSADMKFWKPQTKSIKKIKQYIKNNIEIPDGVEIKFGGL
                     DTLNKAVIPQILASFIAAVFVLFFFMLLYFKKADISILTIAASSLCLFGAFFGEWLFG
                     LDFGMTSVLGVVSLIGIIVRNGIIMYEFAEELRVQKRFSAKEAAMEAGSRRMRPIFLT
                     SATTALGVLPMIISRNPLWMPMGVVICFGVVLSLVIVLSVMPVTYWQIYKHQDLQIQA
                     MPLTDEQETM"
     gene            3518..4885
                     /locus_tag="PK641_03335"
     CDS             3518..4885
                     /locus_tag="PK641_03335"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPG55418.1"
                     /translation="MKKTTKYKLIYILTPLLFIVLNQIDVQAQVLKLSLDSCIQMAVR
                     NNVKVKNANLDIKSAEQEKNAVFTKYFPQISGMAVGFYSDKPLIEYGISDIGNAGIRD
                     VLQSIYSKYGPSLGISDHISFLEKGMITGISAMQPLYMGGRIVIGNKLAELGVDASLY
                     QAEIAKQEVMMQTEELYWQILSLEEKKNTVVNVLELIESLYKDVDCAVNAGLTTQNDL
                     LRVNLKRNEMKSNLIRIENGICLSKMALCQYIGLNRNDSIAIIDSVGMAENPVVYHID
                     NKDAVAQRYESKLLDLSVNAEKLKRRMILGEALPQIGFGGTYAYNNLMEKDYTNLIGF
                     ASIQLPISAWWETSHKLKKHNYLVEKAQNTKNDLTERLELQTQQVYDELDAAYRQITL
                     AEEAVEDAKKNQKLSNDYYHAGLVPLSEMLESQTLYRQAIDQCSDAQINYRIRLSHYK
                     QLIGK"
     gene            complement(4891..6279)
                     /locus_tag="PK641_03340"
     CDS             complement(4891..6279)
                     /locus_tag="PK641_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HPG55419.1"
                     /translation="MIEFLSTIQSFIGNALAWLSSILWGLPMIILLLGTHIILTIRTG
                     FVQRKIGTAILLTFKRDKNSTGDISQFGALMVALAATIGTGNIIGVGIAIAAGGPGAV
                     FWCWLTGVLGIATKYSESLLSVKYRIKTESGKMLGGPMVVLDMVMHKHWLAVFFCIAT
                     ALAAFGIGNMSQANSISTLMNDNFSVSPYITGLVMAFLVGLVMIGGVKWIASVCKVLV
                     PFMALIYVVGCILLLVYNIDYAWDAIKLIITSAFTPKSVAGGFAGGGIMLSMRYGFAR
                     GLFSNESGMGSAPIIASAAKTKNAVRQALISASGTFWDTVVICALSGIVMVTSVLAYP
                     DINFSNGAVLTSMAFGKLHVYGSGILTIALLTFVFSTLLGWSYYAERCLEYLWGSKII
                     KYYHVIWVVCVFIGAVAKLSLVWDFSDCANALMAIPNLIAVISLSGIVANETKKYLWQ
                     NRLDEVDEEMVS"
     gene            complement(6276..>6865)
                     /gene="lptB"
                     /locus_tag="PK641_03345"
     CDS             complement(6276..>6865)
                     /gene="lptB"
                     /locus_tag="PK641_03345"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005852797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HPG55420.1"
                     /translation="TGLIKPNAGKIFLNDLDVSSYPVFRRAQIGIGYLAQEASVFRNM
                     SVENNIKAVLEMTKFSKEYQKDKLESLIEEFHLAKVRKSFGNQLSGGERRRCEIARCL
                     AIEPRFIMLDEPFAGVDPIAVEDIQNIVSKLKDKNIGILITDHNVHETLSITDRAYML
                     FEGKIMFQGTAEELANNPIVREKYLGSNFELRKKQ"
BASE COUNT         2354 a         1293 c         1196 g         2022 t
ORIGIN      
        1 acaataatat aacaagcgga ggaatcgtag aaataggaat ctcagcaaat cctgtttcac
       61 atagctaccc tgtaagaatt aaggctgcca attcaaatca cagaatctta cccggcatga
      121 tatgcaatgt ttatatatcc gacaaaaaca cgaaatcagg attcgtagtt ccgaccaatg
      181 caattttaat tgcaaacgac aatagtaaat ttgtatggct aattgaaaat ggcaaggcag
      241 tacgacgcag tattaaaatt tcagggtata gtggagtcgg agtactcgtt tctgaaggtt
      301 taaatacaaa cgacctcgtc attgttgaag gataccaaaa agtaagcgaa gggatgttag
      361 taacagcaca aacagaacaa taaagaacta gcatgaaaaa aggaataata agtgccagca
      421 taacttatcg cagaataata cacgttgcag tacttttatt gtttgcattc ggcatttatg
      481 ctttgagcaa tatgaataag gatgaattcc cttcttttac attacgccaa ggtattattg
      541 caggtatata tccgggtgca tctgtcgaag aagttgaaaa tcagctaacc aaaccgcttg
      601 aacaattgct gttttcgatg cctgagatta atcgagaaaa tacttattct atatcaaaaa
      661 atggcatcgc gtatgtttac gtcgatctcg atttatctgt aaaagacaaa gatgaaactt
      721 ggtcaaaaat aaggcataaa ttaaatgaac agaaaagttt gacttttcct gcaggtgttt
      781 taggatttgt tgttattgac gattttggaa acaattcttc aatgctgata gctatagaat
      841 ctaacgacaa aacatataaa gagctacact catacactaa tgaattgtca aaacgactac
      901 gacaaatacc cgaaatgggt aatatcaaga ttcttggcga acgtaccgaa gagatcgcag
      961 tcaaaattga tcaatcaaaa ttggcatcat acggattaaa tccacaaact ctgcttctta
     1021 actacgcaag caggagttta cttcttccgg gaggtaacta tcaagcatta gatttcagca
     1081 tgcctgtaca tataaatcaa tctattgcca gcgaaaaaga aatagaagat caaataattt
     1141 atgcctttgc tgatggctca tcaataagac taaaagatat tgcgactgtt gaacgccgtt
     1201 accaagacct taagaatgat gtaaacttta atggtaaaaa tgccatagta gtttcggcag
     1261 aaatgcgtaa aggcaacaat atagttgcct ttggagatga agtaaacaag attctcgacg
     1321 attttagcaa aatcacacca cagagcgtaa aagtatatcg cattacagat caaccaacag
     1381 ttgtcgacaa gtctgtaagt tcatttttac gcgaccttat catatctatg atagtagtaa
     1441 tcgttgttat gttgatgcta tttccactac gtacagctct cgttgcaagt tcgggtttac
     1501 ccatttgtac agcagtttct attggattaa tgtatctatt taatatagaa cttaatactg
     1561 taacactcgc tgcacttata gtagtacttg gtatggttgt tgacgattca ataattaaca
     1621 tagacggata tatagacaaa ctacaaaaag ggtataaacc atttgaagcc gcagtaacta
     1681 gcgcaaaaga attatttatg cctatgctga tttctacagc agctattagt gctatgtttt
     1741 tcccaatgaa gttaattatc aaaggaccac ttggcgattt tattcagtta tttccttgga
     1801 caattttatt ttctttagct tcgtcactga tatatgcaat gcttgttata cctgcattag
     1861 agataaaatt tatcccaaca gctaccgctc agcgtaaacc taacctattt gaacgaggtc
     1921 aggcatattt ctttaatcta atacaagatg gatacgagaa gctacaaaac acatgcttta
     1981 gacatccggt aattacaata acagttggtg tcgcatcaac attattgggt atagttatgt
     2041 ttttgcaact caatattcag atgatgccta tggcagaaag agattgtttt gccatagaaa
     2101 ttcgtttacc agaaggttcg gacatatcag agactaaggt tgtgagtgac tcattgcaaa
     2161 atttactttt gcaagacaaa cgtatcaaat cggtaactgc atttattggc gagccggctc
     2221 cgcgtttcca cataacttac gctcccagta tgccggctaa caatctggca caattaatag
     2281 taaacactca gtctgttgat gccaccgaag aattactgcc tttactcgac aaaaaatatt
     2341 catattattt tcctaaagcg catattagat taaggcagct tgattatcag gcagttacaa
     2401 atccgattga gatacacgtc ttaggtgatg atgataaaaa gattgcagag attgctgaca
     2461 aaataaagtc atatatgcta acactaaaca acgaacttag atgggtacat agcagcaatg
     2521 acgaatattg tccgtctgta aatatcaatt taaaaccgga agagagtgcc agattaggca
     2581 tcagtcaaag tttcttggca ttaaaccttg caggaacctt taatggtttg cctttaagtt
     2641 ctatatggga agatgatatt aaaatcccta taaatctata ttacgccgat tttaacgctg
     2701 atagtagtta taataacata ggtaatcaat taattcctac cgcaatacca aacacatggg
     2761 taccactcag acaagtagca gacgcatcgc ccgaatggca ttcgtcgcaa atcataagac
     2821 gattcgacag aaattgcatt actgttagtg ccgacatgaa attttggaag ccacaaacta
     2881 aaagcatcaa aaagataaaa caatatatta aaaataatat tgaaatacct gatggagtag
     2941 aaattaaatt tggtggactt gatactctta ataaagcggt aattccgcaa atcttagcaa
     3001 gtttcatagc tgctgtattt gtattgttct tttttatgct cttgtatttt aaaaaagcag
     3061 atatttcaat tttaaccatc gctgcaagtt cattatgttt attcggagca ttctttggcg
     3121 agtggttatt tggcttggac tttggtatga cctctgtttt aggagttgtc agccttatcg
     3181 gtattatagt ccgaaacgga attatcatgt acgaatttgc agaagaatta cgtgtacaaa
     3241 aacgattttc tgcaaaagaa gctgcaatgg aagcaggcag cagacgtatg cgccctattt
     3301 ttcttacatc agccacaacc gcacttggtg tattgcccat gattataagt agaaacccat
     3361 tatggatgcc tatgggagtt gtaatttgtt ttggagttgt gttatcttta gtgatagtac
     3421 tttcggtgat gcctgtaacg tattggcaga tttacaagca tcaagattta caaatccaag
     3481 caatgccgct aacagacgaa caagaaacaa tgtaacaatg aaaaagacaa ccaaatataa
     3541 gcttatttat atattgactc cattgctttt tatagtattg aatcaaattg atgtacaggc
     3601 acaagtacta aagctatcgc ttgattcttg catacaaatg gctgtacgaa ataatgtaaa
     3661 agttaaaaat gcaaatcttg atattaaatc agcagaacaa gaaaagaatg ctgtgttcac
     3721 taagtatttc ccacagatat cgggtatggc tgtcggattt tattcagata aaccattgat
     3781 agaatatggg atttctgata taggaaatgc cggaattcgt gatgtactac agagtatata
     3841 tagcaaatac ggaccgtcac taggcattag tgaccacatc agtttcctcg aaaaaggaat
     3901 gattacaggt ataagtgcaa tgcaaccgtt gtacatgggt ggtaggattg ttataggtaa
     3961 caagttggca gaattagggg ttgatgcatc tttatatcaa gcagaaattg caaaacaaga
     4021 ggttatgatg caaacagaag aattatattg gcagatttta tctcttgaag aaaagaaaaa
     4081 tacagttgta aacgtactcg aattaataga atcattatac aaagatgtag attgtgcggt
     4141 taatgccggt ttaactactc aaaacgattt actgagagta aacctaaagc gcaacgaaat
     4201 gaagtctaat ttaattagaa ttgagaatgg aatatgctta tcaaaaatgg cactttgtca
     4261 atatatcgga ttaaatcgta atgatagcat tgctattatc gactcagtcg gcatggccga
     4321 aaatcctgta gtatatcata tcgacaacaa agacgccgtt gcacaacgat acgaaagtaa
     4381 attattagat ttaagtgtaa atgcagaaaa actaaaacgt agaatgatac tgggtgaggc
     4441 tctaccacaa ataggttttg gaggtacata cgcctataat aatctaatgg aaaaagatta
     4501 cacaaacctg atcggttttg ccagcattca acttcctatc agtgcatggt gggaaacatc
     4561 acacaaatta aagaaacaca attacttggt cgaaaaggct caaaatacaa aaaacgatct
     4621 tacagaaaga ttagaattac agactcaaca ggtctacgac gaattagacg ccgcatatcg
     4681 tcaaataaca ttagcagaag aagctgttga agatgcgaaa aaaaatcaga aattgtcgaa
     4741 cgattattat cacgcaggat tagttccctt atctgaaatg cttgaatcac aaacgcttta
     4801 tcgtcaagct attgatcaat gtagtgatgc tcaaattaat tatcgcatca gacttagcca
     4861 ttataaacaa cttataggca aataaaaata tcaactaacc atttcttcat caacctcatc
     4921 taatctgttc tgccagagat atttttttgt ttcattagct actatgccac ttaaacttat
     4981 tactgcaatt aggtttggta ttgccatcaa agcattggcg cagtctgaaa aatcccatac
     5041 caaagataat tttgcaacgg ctccaataaa cacacatact acccatatta catgataata
     5101 ttttatgatt tttgagcccc atagatattc taaacatctt tctgcataat acgaccaacc
     5161 aagtaatgta gaaaaaacga aggttagcaa tgcaattgta agtattccgg aaccatagac
     5221 atgcaattta ccaaatgcca tgcttgttaa tactgcacca ttcgagaaat taatatccgg
     5281 atatgctaat acacttgtta ccataactat acccgacaat gcacatatca caactgtatc
     5341 ccaaaaagtt ccacttgcag atattaaggc ctgacgaact gcattttttg tttttgcggc
     5401 agatgcaatt atcggagcag aacccatacc cgattcattt gaaaacaagc ctcttgcaaa
     5461 accatacctc atagataaca ttatacctcc tcccgcaaat cctcctgcaa cagatttagg
     5521 agtaaatgcc gaggtaataa tcaacttaat agcatcccac gcataatcaa tattatagac
     5581 aagtaaaagt atgcatccta ctacatatat caaggccata aaaggcacta agactttaca
     5641 aacactagca atccacttca cccctcctat cataacaagc ccaacaagaa aagccataac
     5701 gagtcctgtt atatatgggg ataccgaaaa gttatcattc attaatgttg agatcgaatt
     5761 tgcctggctc atatttccta taccaaatgc tgccaaggcg gttgctatac aaaagaatac
     5821 agccaaccaa tgtttgtgca tcaccatatc tagtacaacc atcggtccac ccaacatttt
     5881 accactttct gttttaatcc tatactttac tgataataga gattctgaat acttagttgc
     5941 aataccaagt actcctgtaa gccaacacca aaatactgca ccagggccac cggctgctat
     6001 tgcaatacct actccaataa tatttccggt tcctattgtt gcagccaaag caaccatcaa
     6061 tgctccgaac tgacttatat caccggtcga atttttatca cgtttaaatg taagaaggat
     6121 tgcggttcct atctttcttt gtacaaagcc agtacggata gttagaatta tgtgagtccc
     6181 caacaacaat attatcattg gcagccccca tagaatcgag ctcaaccaag ccagagcatt
     6241 gccaataaaa gattgtattg ttgataaaaa ttcaatcatt gtttttttct tagttcaaaa
     6301 tttgaaccta agtatttttc gcgcacaata ggattattag cgagttcttc agctgttcct
     6361 tgaaacatta tttttccctc aaaaagcata taagctctat cggttatact taatgtttca
     6421 tgtacgttgt gatctgtaat caaaatgcct atgtttttat ctttaagttt agatacgata
     6481 ttttgtatat cctcaacagc aattggatcg acccctgcaa aaggctcatc gagcattata
     6541 aaacgtggtt caatagccaa acatcttgca atttcacagc gcctgcgctc gcctcctgac
     6601 agctgatttc caaaactctt gcgaacctta gctaaatgaa attcttctat caatgattcc
     6661 aatttatcct tttgatattc ctttgaaaat tttgtcatct caagaacagc tttaatatta
     6721 ttctcaacag acatattcct gaatactgat gcttcttgag ccaaataacc aattcctatc
     6781 tgcgcacgtc taaataccgg atacgaagag acatccagat cgtttaagaa gatcttgcct
     6841 gcattcggtt ttattaaacc tgtgg
//
LOCUS       DAOPID010000064         6820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_14330_length_6820_cov_3.836068, whole genome shotgun sequence.
ACCESSION   DAOPID010000064 DAOPID010000000
VERSION     DAOPID010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 6820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6820
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14330_length_6820_cov_3.836068"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2139
                     /gene="ppdK"
                     /locus_tag="PK641_03350"
     CDS             <1..2139
                     /gene="ppdK"
                     /locus_tag="PK641_03350"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HPG55421.1"
                     /translation="RFKTAVKKQTGKDFPEDAYEQLWGAICAVFDSWMNERAILYRKM
                     EGIPAEWGTAVSVQAMVFGNMGDTSATGVCFSRDAATGEDQFVGEYLINAQGEDVVAG
                     IRTPQQITKLGSQRWAKLANVSEADRFAKYPSMEESMPVIYGELDALQTKLENHYKDM
                     QDMEFTVQEGKLWFLQTRNGKRTGAAMVKIAMDMLHQGVIDEKTAVLRVEPNKLDELL
                     HPIFDKAALKVAQVLTKGLAASPGAACGKIVFNAEDAAVWASKGEHVIMVRIETSPED
                     LAGMAAAQGILTARGGMTSHAAVVARGMGKCCVSGAGSLVIDYTAKTLSVNGTTLKEG
                     DFISLNGSTGEVYLGAVATKEAELDEDFAELMKLADKYTRLVVRTNADTPHDAQVARR
                     FGAVGIGLCRTEHMFFEGDKIRAMREMILAETLEGRRKALARILPYQQADFKGIFKAM
                     EGCPVTVRLLDPPLHEFVPHEEKEQRELAQIMGVSYDYIRQRVDSLHEANPMLGHRGC
                     RLGNTYPEITEMQTRAILGAALELKKEGVETHPEIMVPLTGIVNEFANQERIIRETAE
                     KLFQERGVSIEFKVGTMIEIPRAALTADRIATKAEFFSFGTNDLTQMTFGYSRDDIAS
                     FLPAYLDMKILKNDPFQILDQNGVGQLVQMATEKGRSVRPDLKCGICGEHGGEPSSVE
                     FCHRVGLNYVSCSPFRVPIARLAAAQAALR"
     gene            2260..3162
                     /locus_tag="PK641_03355"
     CDS             2260..3162
                     /locus_tag="PK641_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPG55422.1"
                     /translation="MTTDLQQRAREAKKVTLVGFIINLLLSIAKLTAGITGKSAAMVA
                     DAVHSISDFTTDVVVIAFVGVTAKDCDDNHHYGHGKYETFATLLISAALLAVGIGLLL
                     NGVQNSIKVINGESLEKPGIIALIAAIVSVIIKEALYWYTVIVGKKINSKAVIANAWH
                     HRSDAFSSIGTTLGIGGAIFLGEKWRILDPLAGALVSIFIIKVAIDLGLPSIKELLES
                     SIPKKEEEDIIRIITNTDGVKKYHHLRTRKVGNAYAIDVHIKLDRNISFVKSHDIATD
                     LEKQLRAIYGNNTLLSIHTEPLPE"
     gene            complement(3166..4413)
                     /locus_tag="PK641_03360"
     CDS             complement(3166..4413)
                     /locus_tag="PK641_03360"
                     /inference="COORDINATES: protein motif:HMM:NF039309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPG55423.1"
                     /translation="MRKLALENQITMKQISYLLYSIIILFTLYSCNPNNNQCKNDDET
                     ADTVTIIEYGIPIEQYEMEEGIVKKGQTLSDLFASFGALPQITSQLNTLNDSIFNVKN
                     VKAGSTYKVYFTNDSIRVPHYFVYNKSVVDHMVFHLIDSLHIHHYQKPINSILKTSSA
                     VIESSLWNAIEEQNLNRALAIDLSDIYAWEIDFFGIQKGDNFTVLYDELFVDSTSIGI
                     GKIHAVKFNHEGKDYYAYHYSDSIAELDGYWNEKGQNLKKAFLKAPLKFSRISSGFSY
                     ARKHPIYKIVRPHTGVDYSAPKGTPVMAIGDGKVIFKAYKGGGGNTIKIKHNSNYTTA
                     YLHLSNYAKGIKIGSHVKQGQVIGYVGSTGASTGPHLDFRVWKNNTPINPLRMESPSA
                     DPIPSNCMNKFQAICDSLTKYLN"
     gene            4762..6261
                     /locus_tag="PK641_03365"
     CDS             4762..6261
                     /locus_tag="PK641_03365"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HPG55424.1"
                     /translation="MADKLFIFDTTLRDGEQVPGCQLNTVEKIQVAKALEALGVDVIE
                     AGFPVSSPGDFNSVVEISKAVTWPTICALTRSVEKDIDAAAEALQYAHKKRIHTGIGT
                     SDYHIKYKFNSTREEIIERAIAATKYAKKYVEDVEFYCEDAGRTDNEYLARVVEAVIK
                     AGATVVNIPDTTGYCLPSEYGEKIKYLMENVSNIDKAIISTHCHNDLGMATANTMSGI
                     INGARQVEVTINGVGERAGNTSLEEVAMILKCHKHLNVETQINTQKIISVSRLISNLM
                     NMPVQANKAVVGRNAFAHSSGIHQDGVLKNMQTYEIMNPKDIGIDDNSIVLTARSGRA
                     ALKHRLNILGVDVDENKLDKIYKDFLDLADKKKDIHDDDILVLAGIERANGKKIQLDY
                     LHVTSGKGIKPLAYIGLIIAGEHFEASSGGNGPVDAAIKALKLIISREMTLQEFTIQS
                     INKGSDDMGKVHMQVLYDGKVYYGFSANTDIVVASIEAYIDAINKFVEK"
     gene            6295..6759
                     /locus_tag="PK641_03370"
                     /pseudo
     CDS             6295..6759
                     /locus_tag="PK641_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
BASE COUNT         2263 a         1044 c         1361 g         2152 t
ORIGIN      
        1 cgtttcaaga cagctgttaa aaaacaaaca ggcaaagatt tccctgaaga tgcatacgag
       61 caattgtggg gcgcaatctg tgctgtattt gatagctgga tgaacgaacg tgctatatta
      121 tatcgtaaaa tggaaggaat acctgcagaa tggggtacgg cagttagtgt acaagccatg
      181 gtattcggaa atatgggcga tacatcagca acaggtgtat gcttctcacg tgatgctgca
      241 accggtgaag atcaattcgt aggtgagtat ttgattaatg ctcaaggtga agatgttgta
      301 gcaggtattc gtactccaca acaaattaca aaattaggtt cacaacgctg ggcaaaatta
      361 gctaatgtat cagaagctga tcgttttgca aaatatcctt caatggaaga atcaatgcct
      421 gttatatatg gagaattaga cgcacttcaa acaaaattgg aaaaccacta caaagatatg
      481 caagatatgg aatttacagt tcaagaaggt aaattgtggt tcttgcaaac tcgtaacgga
      541 aaacgtacag gagcagctat ggttaaaatt gccatggata tgttacatca aggtgttatt
      601 gatgaaaaaa cagctgtatt gcgcgttgaa ccaaataaat tggacgaatt acttcaccca
      661 atatttgata aagctgcatt aaaggttgct caagtgctta caaaaggctt ggctgcttct
      721 ccgggagctg cttgcggtaa gattgttttc aatgctgaag atgctgctgt atgggcaagc
      781 aaaggcgaac atgttataat ggttcgtatt gaaacatctc ctgaagactt ggctggtatg
      841 gctgctgcac aaggtatatt aactgcacgt ggcggtatga catcacacgc tgctgtagtt
      901 gctcgtggaa tgggcaaatg ctgcgtatca ggtgcaggat cattagttat tgattataca
      961 gcaaaaacac tttctgtaaa cggaaccact ttaaaagaag gtgattttat ttcattaaac
     1021 ggatcaaccg gtgaagttta tttaggtgct gttgcaacta aagaagctga acttgatgag
     1081 gatttcgcag aattgatgaa attagctgat aaatatactc gtttggtagt tcgtaccaat
     1141 gctgatactc ctcacgacgc acaagttgct cgtcgttttg gtgcagtagg tataggactt
     1201 tgtagaacag agcatatgtt ctttgaaggt gacaagatcc gtgcaatgcg cgaaatgatt
     1261 ttggctgaaa ctctagaagg acgtcgtaaa gcattagcaa gaattcttcc atatcaacaa
     1321 gcagacttta aaggaatctt taaagcaatg gaaggatgtc ctgttacagt acgtttgtta
     1381 gatccacctc tacatgagtt tgttcctcac gaagagaaag aacaacgtga attagcccaa
     1441 ataatgggtg tttcttacga ttacattcgt caacgtgttg attctttgca cgaagccaat
     1501 cctatgttag gccatcgtgg ttgccgttta gggaatacat atcctgaaat cacagaaatg
     1561 caaacacgtg ctattttagg tgcagcactt gaattgaaaa aagaaggtgt tgaaacacat
     1621 cctgaaataa tggttccatt aacaggtatt gtgaacgagt ttgctaatca agagagaata
     1681 attcgcgaaa cagctgaaaa attatttcaa gaacgcggag ttagcatcga atttaaagta
     1741 ggtactatga ttgaaattcc acgtgcagca cttactgctg atcgtatcgc tacaaaagcg
     1801 gaatttttct cattcggaac gaatgactta actcaaatga catttggtta tagccgtgat
     1861 gatattgcat cattcttgcc tgcttatttg gatatgaaga tcttaaaaaa tgatccattc
     1921 caaatactag accaaaatgg tgttggacaa ttagtacaaa tggctacaga aaaaggccgc
     1981 agcgtacgtc ctgatcttaa gtgtggtatt tgtggtgaac atggtggcga gccttcgtca
     2041 gtagagtttt gccatagagt aggcttaaat tatgtttcat gttctccatt ccgtgtgcct
     2101 atcgcaaggt tggcagcagc acaagctgca ttgcgttaat gtgagaatat atacaataat
     2161 agaaaaacac cgacataatt gttggtgttt ttctatctaa tatagtcaag tattttaagc
     2221 agcgttttta ttaactaact aataattatt taattcatta tgacaacaga tttgcaacag
     2281 cgcgcacgtg aagcaaaaaa agtaacgctg gtaggtttta taattaatct tttattaagc
     2341 atcgcaaaac taacagccgg tataaccggt aaaagtgctg caatggttgc tgatgccgta
     2401 cattcaatat ctgattttac tacagatgtg gttgtaattg catttgtagg tgtaactgct
     2461 aaagattgtg atgataacca tcattatgga catggtaagt atgaaacatt tgcaacatta
     2521 ttgattagtg ctgctttgct tgctgtcgga atcggtttac tattaaatgg agttcagaat
     2581 tcaattaaag taattaatgg agaatcatta gaaaaaccag gaattatagc cttgattgct
     2641 gctatagtat ctgttattat taaagaagct ctttattggt acactgtgat agttggtaaa
     2701 aaaattaata gcaaggctgt tatcgcaaat gcatggcatc atagatctga tgcattttct
     2761 tcaataggta cgaccttagg tattggaggc gcaatatttt taggagaaaa gtggagaata
     2821 cttgatcctc ttgcaggagc attagttagt atttttatta taaaagtagc cattgattta
     2881 ggtttaccaa gtattaaaga attacttgaa tcctcaattc caaaaaaaga agaagaagat
     2941 attattcgta ttattactaa tactgatgga gttaagaagt accatcattt acgtactcgt
     3001 aaagttggca atgcttatgc cattgatgta catataaaat tagaccgaaa tatcagtttt
     3061 gtaaaatcac atgatattgc taccgatttg gaaaaacaat tgagagccat atatggtaat
     3121 aacactcttt taagcataca tacagagccg cttccggagt agttatcaat taagatattt
     3181 tgtcaaacta tcgcatatag cttgaaattt attcatgcaa ttagaaggaa tgggatcagc
     3241 tgagggagat tccattctta atgggtttat gggtgtgtta tttttccaaa ctctgaaatc
     3301 gagatgtggc ccggtagatg caccggtact acctacatat ccaatcactt gtccttgttt
     3361 aacgtgagat cctattttta ttccttttgc ataatttgat aggtgcagat aggctgttgt
     3421 gtaattacta ttatgtttaa tcttaatggt atttcctccg ccacctttat aggctttaaa
     3481 aataaccttt ccatcgccaa ttgccattac aggagtgcct ttcggagcag aataatctac
     3541 acctgtgtgc ggacgtacta ttttgtatat aggatgtttt ctggcgtatg aaaaaccgga
     3601 agatatcctt gaaaatttta atggggcttt taaaaatgct tttttaaggt tttgtccttt
     3661 ctcattccaa tatccgtcta attcagctat tgaatcagaa tagtgatatg cataataatc
     3721 ttttccttcg tgattaaatt ttactgcatg aattttacct attccaatag aagtactatc
     3781 aacaaataat tcatcataca atacagtaaa attgtcgcct ttttgtatac caaagaaatc
     3841 aatttcccat gcgtatatat cagataaatc tatagctaat gcgcgattta aattttgttc
     3901 ttcaattgca ttccataagg atgattcaat aactgcagaa gaagttttaa gtatgctgtt
     3961 tataggtttt tgatagtgat gtatatgcaa gctgtcaatt aaatgaaaaa ccatatgatc
     4021 aactacagat ttattataca caaagtaatg tggaaccctt atagaatcat tagtaaaata
     4081 aactttatag gtgctacctg cttttacatt tttgacatta aatatagaat cattaagtgt
     4141 atttaattgg ctggtaattt gcggcaaagc gccaaaagaa gcaaataaat cagatagtgt
     4201 ttgtcctttt tttacgattc cttcttccat ttcgtattgt tcaataggaa tgccgtattc
     4261 tataattgtt acagtgtcag ctgtttcatc atcatttttg cattgattat tgtttgggtt
     4321 gcaagagtat agggtgaata gaattattat tgaatataat aagtatgata tctgtttcat
     4381 tgttatttga ttttcgagtg caagtttacg cattattgta cagaaaagca aaataaattg
     4441 catatggtaa aaatatgggt ctttataatt taattcttgt ttttgtagct atattgtaat
     4501 tattttgatg taaataactg ttgtttataa caaattcatg tattttacta ataatttatt
     4561 tgtgtattta aaaatatact tatatctttg catcgttaaa aaaatacaga tgacaaattt
     4621 ttcaatcata ctaagcatta ttattctctt gcgacgcaat gggaagtaag tttggtatgt
     4681 ataactctaa agacctttct tatttcccga tttgaaaggt ctttttttat gctttaaaaa
     4741 caacttaata aatacaataa aatggcagac aaattattca tttttgacac aacattaaga
     4801 gatggcgaac aggtacccgg atgccagtta aatactgttg aaaaaattca agtagcaaaa
     4861 gcacttgaag cacttggagt ggatgtaatt gaagcaggat tccctgtttc cagtcccggt
     4921 gattttaatt ctgttgttga aatctcaaag gcagttacat ggcctacaat atgcgctttg
     4981 actagaagtg tagagaaaga tattgatgca gcagccgaag ctttgcaata tgctcacaaa
     5041 aagcgtattc acaccggaat tggaacgtcc gattatcaca taaagtataa gtttaattca
     5101 acacgtgaag aaattattga acgtgcaatc gcagctacaa agtatgcaaa aaaatatgtt
     5161 gaagatgtag aattttattg tgaagatgcg ggacgtacag ataatgagta tttggcacgt
     5221 gtggttgaag ctgtaattaa agcaggagca accgttgtaa atatacctga tacaaccgga
     5281 tattgtttgc cttcagagta tggtgagaaa attaaatatc taatggaaaa tgtttcgaat
     5341 attgataaag caataatctc tacacattgt cataacgatt taggtatggc aacagccaat
     5401 acgatgtcag gtattattaa tggtgcaaga caagttgaag ttacaatcaa cggagtaggt
     5461 gaacgtgcag gaaatacttc tttggaagaa gttgcaatga tacttaagtg tcataaacat
     5521 ttaaatgtcg aaactcaaat taatacacaa aaaataatat ctgtaagcag acttatttca
     5581 aatcttatga atatgccggt acaggctaat aaggccgtgg taggacgtaa tgcatttgct
     5641 cactcgtcag gcatccatca agatggtgtg cttaagaata tgcagacata cgaaattatg
     5701 aatcctaaag atattggtat tgatgataat tctattgttc tgacagcacg tagtggaaga
     5761 gcagcattaa aacatcgtct taatatatta ggagtagatg tagatgaaaa taaattggat
     5821 aagatttata aagacttttt ggatttagcc gataaaaaga aagatataca tgatgatgat
     5881 atacttgtat tggcgggaat tgaaagagct aatggtaaga aaatacagtt ggattatttg
     5941 catgtaacat caggtaaggg gataaaacct ttagcataca ttggtttaat tatagcaggc
     6001 gaacattttg aagcatcatc aggaggtaat ggacctgtag atgcagcaat aaaagcctta
     6061 aaacttatta taagcagaga aatgacctta caagaattta ctattcaatc aataaataag
     6121 ggtagtgatg atatgggtaa ggtacacatg caagttttgt acgatggaaa ggtatattat
     6181 ggctttagtg ctaatactga cattgttgtt gcatcaattg aagcatatat agatgcaatc
     6241 aacaaatttg tagaaaaata aaattacatt tgtagttaaa atactaataa agttatggga
     6301 aaaacattat ttgataaaat atgggaatcg catatagtat ctaaggttga aggcggtcct
     6361 acacagttat acatagacag aatgtattgt catgaggtga caagcccaca agcattcgat
     6421 ggattacgta aacgaggatt aaatgtattt cgtcctaagc aggtaacatg tataccggat
     6481 cacaatattc caacaattaa tcaagaatta cctattaagg atcctgtttc aaaaaatcag
     6541 gttgatacgc tcgacaaaaa tgctaaagac tttaaagttg agtattaccc tatgggacac
     6601 tctaaaaatg gtatcataca tgttgtcggg cctgaaaatg gtctgtcttt acccggtatg
     6661 actattgtat gtggtgactc tcacacatct acccatggtg caataggagc tatagcattc
     6721 ggcattggta cctcagaagt cgaaatggtt ttggctagtt agtgtgtttt tcaatcacgt
     6781 ccggaaacta tgcgtattaa ttttaatggt aaactaaagg
//
LOCUS       DAOPID010000065         6743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_14565_length_6743_cov_4.846292, whole genome shotgun sequence.
ACCESSION   DAOPID010000065 DAOPID010000000
VERSION     DAOPID010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 6743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6743
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14565_length_6743_cov_4.846292"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..583)
                     /locus_tag="PK641_03375"
     CDS             complement(38..583)
                     /locus_tag="PK641_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55425.1"
                     /translation="MKIASTIKVFILLALCAFCFTSCTTESYAEKRNKEKKAWADFKT
                     ANNLEISTDSAYCFNRETPWPENLYFQTHRGAFVRLIKDDTNQRHPSEGNRTIIRFLS
                     YDLNWNLIGGNTGTSSREGEVSVYSPNGTDPCVGWNDVIPCLHHGSEAIMIIDSQLGP
                     TDQQTNVITTIVHITDFSVAN"
     gene            complement(587..1054)
                     /locus_tag="PK641_03380"
     CDS             complement(587..1054)
                     /locus_tag="PK641_03380"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPG55426.1"
                     /translation="MPPQIPSNQEAIDTERQNMLIYNQLCIEAEQKDIKQFIDSSEYK
                     MTYSEDGFWYYIVNKGNGGKIRNGQIVKIAYSLELLDGTVCYSADKDGIKTFEVGKRK
                     VEKGLDIAMPLFAENSEAIIVIPSHLAHGVVGDRNCIPARTPILYRIKILSVK"
     gene            complement(1113..3542)
                     /gene="thrA"
                     /locus_tag="PK641_03385"
     CDS             complement(1113..3542)
                     /gene="thrA"
                     /locus_tag="PK641_03385"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HPG55427.1"
                     /translation="MKVLKFGGTSVGSVENILKVKDIVFSVSEPVIVVVSAISSVTDK
                     LISTAKKASQGDSTYSEDFKIIAEKHAEIINKVVPATHLNEVERQVAIYLDELYSIYR
                     NVYQSRKLDESTLNSALSYGERISSYFMSFVLEAKLFDAVNIIKTKVYHKKNILDKSN
                     TYNNVQKAFANTQQRSIIGGFIASDKSNDSITNLGRGGSDYTASIIAGALDASKLEIW
                     TDVDGFMTADPHLVNNAYTIEELSYIEAMELSNFGARVIYPPTIYPAYERNIPILIKN
                     TMKPSVKGTRITNLNNPEGKAIKGISSISNTCLITLQGMGMVGVLGISSRTFQALANE
                     GISVFLISQASSENSTTFAVRNEEADIAVKALSDEFKNELERGEIWSITPQKDLATVA
                     IVGENMKYNTGLAGKLFNTLGRSGINIIACAQGASETNISFVVMQKDLQKALNVLHDS
                     FFLADYQVLNIFQVGTGTVGGSLLEQIEHQQEKILKQNSVKLNVVGITDINGSIFNEH
                     GINLKNYKEEVLKNGKPTAPEDIKRTIIDMNLFNSVFVDCTASPSIAALYEDLLNHNI
                     SVVAANKIAASGSYTNYENLKNIARKKGIKYLFETNVGAGLPIINTMNSLINSGDRIL
                     KIEAVLSGTLNFIFNTISEDIPFSKTILMAKEARFAEPDPRIDLSGTDVVRKLVILAR
                     ESGYKVEQADVKKNLFVPNKYFEGSVEDFWKNIHELDADFEVQRKKLAAENKKYRFVA
                     TMKEGNCEVGLQAVDSSHPFYDLEGSNNIVMIQTERYFDDPMIIKGYGAGASVTAAGV
                     FGDIISIANIR"
     gene            3705..5432
                     /locus_tag="PK641_03390"
     CDS             3705..5432
                     /locus_tag="PK641_03390"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HPG55428.1"
                     /translation="MDTFNFLNMESSEEKELSLAEIVDRLKVATEGENTRQEIAQLKA
                     LFYRRQEALLSEMRQALTESGKETANFVHTFEEEASFKEILAQYKAKRALELEQSEIM
                     NERAYAIKKGILEKLHQLIESSDSVNKTFPQVRQLQQEWKDAGQVAPSKYGELVKQYQ
                     MYMEQFYDFVKISNELRDYDFKKNLELKTALCEAVEKLAKEDNLNDAFQKLQQLHAEW
                     KELGPVAPELRDSLWNRFKSASDIINKKHAEFYQGKKVKEEENLEKKTALCEAVAQIP
                     YSEANTYKAWDEITAKVLELQEAWKKIGFAPKKANTQIYEKFRKACDDIFKAKSQFYK
                     EVKEISASNLEKKRALCEKAEALKDSTEWKETADKFIKLQQEWKTIGPVSKKVSDQIW
                     TRFITACDAFFAAKEKAQSGRVAEQVENLNKKKAVLEKIAAYAENSFAGLKALIAEYN
                     AIGFVPFKDKDGLYSEYKKVLNKQFDRLRVDEIERKLSLFAGSVENAADKSVATLRRE
                     RERLIRTYESLTSDITTRENNMGFINIDSKKANPFMAEMQKVLLRQKEEQEVLLKKIK
                     LLEDNINNA"
     gene            5542..6342
                     /locus_tag="PK641_03395"
                     /pseudo
     CDS             5542..6342
                     /locus_tag="PK641_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005648211.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
     gene            6335..>6743
                     /gene="queC"
                     /locus_tag="PK641_03400"
     CDS             6335..>6743
                     /gene="queC"
                     /locus_tag="PK641_03400"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HPG55429.1"
                     /translation="MNKQDKALVVFSGGQDSTTCLLWAIERFEKVYAVTFDYEQRHKK
                     EIECASNIAKDFGIEHYVLDMALLNQLAPSSLTRSDIPVDEMPDTKTPPNSLVEGRNM
                     LFLTFAAVLAKTKSCRHLITGVCETDFSGYPDCR"
BASE COUNT         2213 a         1091 c         1224 g         2215 t
ORIGIN      
        1 ttccggaaat cgatttgatt agtgtcatgt ttttgcatta atttgcaact gaaaaatcag
       61 taatatgtac tattgtagta ataacattag tttgttggtc ggtaggacct aattgtgagt
      121 caataatcat aattgcttcg ctaccatggt gcaaacaagg tattacatca ttccaaccaa
      181 cgcacggatc agttccatta ggagagtaaa cacttacttc gccttcgcgc gatgaggttc
      241 cggtatttcc tccaattaaa ttccaattaa gatcatacga taaaaatctt attatggttc
      301 tatttccctc agaagggtgg cgttgattgg tatcatcttt tatcaacctt acaaaagctc
      361 ctctgtgtgt ttgaaaatat aaattttcgg gccatggtgt ttctctgtta aaacaataag
      421 cagagtcggt agaaatctct agattatttg ctgttttaaa atcagcccat gctttttttt
      481 ctttattacg cttttctgcg tatgattctg tagtacaaga agtaaagcag aatgcacaaa
      541 gtgcaagcaa aataaatact tttatggttg atgctatttt catgaattat ttaactgata
      601 aaattttgat tctatataat attggagtcc ttgccggaat acaattccta tccccaacaa
      661 ccccgtgtgc caaatgcgat ggtataacaa taattgcttc tgaattttct gcaaataaag
      721 gcattgcaat atctaaacct ttttcaactt ttcgtttgcc gacttcaaat gtctttattc
      781 catctttatc agccgaatag cagactgtgc catctaataa ttcaagagaa taggcaattt
      841 ttacaatttg tccgtttctt atttttcccc cattaccttt gttgacgatg taataccaaa
      901 agccatcttc ggaatatgtc attttatact cagagctatc tataaattgc tttatatctt
      961 tttgttctgc ttcaatacat aattgattgt atatcaacat attttgtctt tctgtgtcta
     1021 ttgcctcttg gttacttggt atttgtggtg gcatttttct atgacatgca gaacaaataa
     1081 tcaaaaaaca cagtaagata tatttttgca tattatctaa tattggcaat acttataatg
     1141 tcaccaaata caccggctgc tgttacactt gcaccggcac catagccttt aataatcata
     1201 ggatcgtcaa aataacgttc cgtctgaatc atgacaatat tgttgcttcc ttccaaatcg
     1261 taaaatggat gtgaagaatc aacggcttgt aatcctactt cgcagtttcc ttctttcata
     1321 gtagccacaa aacgatattt tttgttttct gctgctaatt ttttgcgttg aacttcaaaa
     1381 tcagcatcaa gttcgtgtat gtttttccag aagtcttcaa cagatccttc gaaatattta
     1441 ttaggaacaa atagattttt ctttacatca gcttgttcaa ctttgtaacc cgattcgcgt
     1501 gctaatatca ccaatttacg tacaacatca gtaccactta ggtcaattcg cggatcaggt
     1561 tcagcaaaac gagcttcttt tgccattaat attgtctttg aaaatggtat atcttcacta
     1621 attgtattaa atataaagtt caaagtacct gataaaacag cctctatttt aagtattcta
     1681 tcaccactgt ttattaagct attcatagtg ttgatgattg gtaaacctgc accaacattt
     1741 gtttcgaata aatatttaat acctttttta cgtgctatgt tttttaggtt ctcgtaattt
     1801 gtgtacgaac ctgatgctgc tatcttgtta gctgctacaa ctgatatatt atggtttagt
     1861 aaatcttcgt ataatgctgc aattgaagga cttgctgtgc aatcaacaaa tacagaatta
     1921 aataaattca tgtcaataat tgtacgcttt atatcttccg gtgctgtcgg tttcccgttt
     1981 ttaagaacct cttctttgta attttttaga ttgattccgt gttcgttaaa aatagaacca
     2041 ttaatatcag ttattccaac aacattaagt tttacactat tttgttttaa aatcttttct
     2101 tgttgatgtt caatttgttc cagtaaacta ccaccaactg ttcctgttcc aacttggaaa
     2161 atatttaata cttgataatc agctaaaaag aatgaatcat gcagcacgtt cagagctttt
     2221 tgcaaatctt tctgcatcac tacaaatgaa atatttgttt ctgaagcccc ttgtgcacaa
     2281 gcaataatat taataccgct gcgtccaagt gtattgaata atttcccggc taagccggtg
     2341 ttgtatttca tgttttcgcc aacaattgca actgtagcta agtctttttg gggtgtaatt
     2401 gaccaaattt ctcctctttc caactcgttt ttaaattcat cagacagagc ttttactgct
     2461 atatcagctt cttcattgcg tactgcaaaa gttgttgaat tttcggatga tgcttgagat
     2521 attaagaata cgcttatacc ttcgtttgct aatgcctgaa atgtacgtga actgatgcct
     2581 aatacaccaa ccatgcccat accttgcaaa gtgattaagc aagtgttact aattgatgaa
     2641 atacctttta tagctttgcc ttcaggattg tttaaattag taattcttgt tcctttaact
     2701 gatggcttca tagtattctt gatcagtata ggaatatttc tttcgtatgc aggatatata
     2761 gtaggagggt atattactct agctccgaag ttagataatt ccatagcttc gatgtaagat
     2821 aattcttcta ttgtgtatgc attgttaacc aaatgagggt ctgcagtcat aaatccgtca
     2881 acatctgtcc atatttctaa ttttgaagca tctaaagctc cggctataat agatgcagta
     2941 tagtcagaac ctccacgtcc aagatttgta atagaatcat tagatttatc agatgctata
     3001 aatccaccga taattgaacg ttgttgagta tttgcaaatg ctttttgaac gttattgtag
     3061 gtgttcgatt tgtcaagaat atttttcttg tggtatactt tagtttttat aatgttaaca
     3121 gcatcgaaca attttgcttc taatacaaat gacataaaat atgaagaaat acgctctcca
     3181 taacttaaag cagaatttaa tgttgattcg tctaatttgc gagattgata tacattgcga
     3241 tatatgctgt ataactcatc caagtatatg gcaacctgac gctcaacttc atttaaatga
     3301 gtagcaggaa ctactttgtt aattatttca gcatgctttt ctgctattat tttaaagtct
     3361 tcagaatatg tagaatcgcc ttgagaagct ttctttgcag tgcttataag tttatctgtt
     3421 acactactta tggccgaaac taccacgata accggttctg atactgagaa tacaatgtct
     3481 tttactttaa ggatgttttc tacggaacct acggatgttc cgccgaactt caatactttc
     3541 atgtttttta tatatataat ttgaataagc taaaaatcac gcaatattac tgcttttttc
     3601 gcgtatatgg aaatctatag gtataaaata ataaaaaacg atgcataagc atcaaactac
     3661 aattaaaaag gtgtatattt gcggttttgt agtactgtat ccgcatggat acttttaatt
     3721 ttttgaatat ggaatcatcg gaagaaaaag aattgtcgtt ggcagagatt gttgatcgtt
     3781 tgaaagtagc aactgaagga gaaaataccc gccaggagat agcccaatta aaggctttgt
     3841 tttacagacg acaagaagct cttttgtcag aaatgcgtca agccttgaca gaatcgggta
     3901 aggaaacggc caattttgtt catacatttg aagaagaagc aagttttaaa gaaatattag
     3961 ctcagtataa ggctaagcga gctttggagt tagaacagtc agaaatcatg aacgagcgcg
     4021 cttatgcaat taaaaaaggt atacttgaaa aattgcatca gttaattgaa tcatcagact
     4081 cagtaaataa aacatttcca caagtaagac agctgcaaca agagtggaaa gatgcaggtc
     4141 aggttgctcc aagtaaatat ggagaattgg taaaacaata tcagatgtat atggagcaat
     4201 tttacgattt cgttaaaatt agcaacgaat tgcgtgatta tgattttaaa aagaatttgg
     4261 aactaaaaac tgctctttgc gaagcagtag aaaagctggc aaaggaagat aatttgaatg
     4321 atgcattcca aaaattgcag caattacatg cagaatggaa agaacttgga cctgtagctc
     4381 ctgaactacg tgatagtttg tggaatcgct ttaaatctgc ttctgatatt atcaataaaa
     4441 aacatgcaga attttatcaa gggaaaaaag taaaagaaga agaaaatctt gaaaagaaaa
     4501 ctgctttatg tgaagctgta gctcaaatac cttattcaga agctaataca tataaggcat
     4561 gggatgaaat tactgccaag gtattagaat tacaagaggc atggaaaaag atagggtttg
     4621 ctcctaaaaa agccaacacg caaatttatg aaaaatttcg taaggcatgt gatgatattt
     4681 tcaaagcaaa aagtcagttt tacaaagaag taaaagaaat atctgcatca aatttagaga
     4741 agaaaagagc tttgtgcgag aaggcagagg ctctaaaaga tagcactgaa tggaaagaga
     4801 cagcagataa atttatcaag ttgcaacaag aatggaaaac aataggtccc gtttcaaaaa
     4861 aggtgtcgga tcagatatgg actcgtttta ttactgcatg tgatgcattt tttgcagcaa
     4921 aagaaaaagc acaatcagga cgtgttgctg agcaggttga aaatttgaat aagaagaaag
     4981 cagtactaga aaagattgct gcgtacgcag aaaactcgtt tgccggactt aaagctctta
     5041 ttgccgagta caatgcaatt ggttttgttc cttttaaaga taaagatggc ttgtactcag
     5101 aatacaaaaa agttttgaat aaacaatttg atcgtttaag agttgatgaa atagagcgta
     5161 aactaagttt gtttgcaggt tctgtagaaa atgcggctga taaatctgta gctactttac
     5221 gaagagagag agaacgctta atacgtacat acgagagcct gacttctgat attactaccc
     5281 gcgaaaacaa tatgggtttt attaatattg attctaaaaa agcgaatcct tttatggcag
     5341 aaatgcaaaa agttttgtta aggcaaaaag aagagcagga ggtacttttg aaaaaaatca
     5401 aactattgga ggataatata aacaatgcgt aataatcagt ttaataaaga ttcagaatcg
     5461 acagggaatg gccgtaaacg tataggaaaa tcaaaacctc agtttaaacg caataataaa
     5521 gaaaatgata gagttgaaaa acctaatgct aagttttctg attctgaaaa atttaaacct
     5581 aagagagttt tccccaaaaa gcagaaagta gagcatagcg atgacggctc tattcgcttg
     5641 aatcgttttt tagctaatgc aggagtctgt tctcgcagag aagccgatga atttattcag
     5701 gccggtgttg ttaaaatcaa cggtaaagtt gtttctgaat taggtgtaaa ggttatgcca
     5761 ggtgattcag tgcattttca cgatcagttg attagagcag aacggaaagt atatatatta
     5821 ctcaataaac ctaaaaatac agttactact gctgatgatc ctcaaggtcg tttgactgta
     5881 gtcgatttag ttcgtaatgc ttgtcgcgaa agaatatatc ctgtaggtcg tttagatagg
     5941 aataccatgg gagttttatt acttaccaat gacggagatt tgactataaa acttacgcat
     6001 ccgaagtacg aaaagaaaaa aatatatcaa gtaacactcg ataagtcttt agatcctgaa
     6061 gattttgaaa aaattctatc aggtataact ttagaagatg gtcctataaa tgcagatgct
     6121 cttgaatatg ttgaaaatac ttctagaaaa gaagtgggaa tcgaaataca ttcaggaagg
     6181 aatcgtcttg taagacgtat ttttgaacat ttaggatata aggttgaaaa attagacaga
     6241 gtatattttg caggtcttac taagtataat ttacctcgtg gtaagtggcg ttttttaact
     6301 gatgcagaag tatcagcttt gaaaatgggt tcgtatgaat aaacaagata aagctttggt
     6361 tgtttttagc ggtggacagg attctacgac ttgtctttta tgggctatag aaagattcga
     6421 aaaagtatat gctgtaactt tcgattacga acaaaggcat aaaaaggaga ttgaatgtgc
     6481 aagtaatatt gccaaagact tcggtattga acattatgta cttgacatgg ccttgttaaa
     6541 tcaattagca ccaagttctc ttactcgttc tgatattccc gttgatgaaa tgcctgatac
     6601 aaaaactcct ccaaatagtt tagttgaagg tcgaaatatg ttgtttttga cttttgctgc
     6661 agtattggca aagacaaagt cttgtagaca tcttattaca ggagtgtgcg aaacagattt
     6721 tagtggttat cctgattgca gag
//
LOCUS       DAOPID010000066         6683 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_14752_length_6683_cov_3.929240, whole genome shotgun sequence.
ACCESSION   DAOPID010000066 DAOPID010000000
VERSION     DAOPID010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 6683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6683
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14752_length_6683_cov_3.929240"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..344
                     /locus_tag="PK641_03405"
     CDS             117..344
                     /locus_tag="PK641_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55430.1"
                     /translation="MRSINVSNVNAEDSVGFCHLAFDMENKDALIAKMIELKAKNVRV
                     ISDARVTGDGYFESVIADFDGNKIELSTTQF"
     gene            355..1698
                     /locus_tag="PK641_03410"
     CDS             355..1698
                     /locus_tag="PK641_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006046071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HPG55431.1"
                     /translation="MKELKCPKCGNVFTVDEADYASIVNQVKNAEFSEELNLRVAELT
                     KQLATENEKSTLELTKNHLAELNRKNVEISKLSAEKAGIASLTQNEFNEKLSEKDKEI
                     SALTAKVTETQLKIQLAVYEEQNKNMSELNDKDMQIERLKICIENQKQTEELAKTSLE
                     RKYQEELKQMKEMLEYYKDLKARMSTKMVGETLESHCNAEFGRLRQMFPYAYFEKDND
                     ASSGSKGDFIFRDYEDEESMKRKSEYVSIMFEMKNEMDETATKHKNEDFLKKLDEDRT
                     TKKCEFAVLVSLLEPDSDLYNTGIVDMSHRYPKMYVIRPQFFLPLLTLLIQTSKKSME
                     YKKALTIAQSQSVDVSNFENQLNDFKDKFGNNYRLASEKFKVAIDEIDKSIDHLQKIK
                     SNLLGSENNLRIANDKASNLTIKRLTRDNATMKAKFDEARKNSPNAIIDDTDTVD"
     gene            complement(1969..2358)
                     /locus_tag="PK641_03415"
     CDS             complement(1969..2358)
                     /locus_tag="PK641_03415"
                     /inference="COORDINATES: protein motif:HMM:NF013996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HPG55432.1"
                     /translation="MKEQVKFFRCNICGNLVAVVNSSGVPMVCCDEEMEELQANTVDA
                     STEKHLPVVTKVSDGVWNVKIGSTPHPMVPEHFIDFLYFETTSGGKLVYLDHAGEPSA
                     EVCLCDDKLVAVYAYCNLHGLWKTEIK"
     gene            complement(2477..3169)
                     /locus_tag="PK641_03420"
     CDS             complement(2477..3169)
                     /locus_tag="PK641_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HPG55433.1"
                     /translation="MIGTLINTAAIIAGGFIGMIFKKNLAEKYVNIYFQAVGIFTIML
                     GVKMGFGMNNPLIVVLSLACGGLCGEYLKLEERTENLSNKLKRRLHAKNEQFTEGFIT
                     STLLFCMGSMSVIGAIEEGFGKTSDLLLTKSILDGFSAMLLASAFGSSVLFASISVLF
                     YQGILTLFVYLFARDIPNEYIVAITSVGGILLIGLGINILKIKNLKIVNLLPSLVFIC
                     FFLFVYRFLALL"
     assembly_gap    3171..3270
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3275..3620
                     /gene="rnpB"
                     /locus_tag="PK641_03425"
     ncRNA           3275..3620
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK641_03425"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            3635..4558
                     /locus_tag="PK641_03430"
     CDS             3635..4558
                     /locus_tag="PK641_03430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HPG55434.1"
                     /translation="MKKIIEYCKEIYKFITEKIWKMPAEQQDKNFLIRLIMVIYLAIT
                     RYIKDELAVRASALTYYTIFAVVPFLCLIIGIGKHFGVQHYIESELILMFPGQEEMLI
                     HAFEIVDSYLMQIHGGFLLVAVILLLWTLYGVVTQVEVAFNNIWRVHQNRKIYYRVGG
                     LLLMMVLFPIFLFVSGVVSIYMNTTLSSLNIFLKPVWNNILELFPSFINVILLLMLYL
                     VMPKTKVRFVPAFIGALLAGIVLAVFQQLYISGQIWVAHYNAIYGSFAAIPLLLLWLQ
                     FSWMIILVGAELSHCIQNRHNLGKEIVADNK"
     gene            complement(4571..5329)
                     /locus_tag="PK641_03435"
     CDS             complement(4571..5329)
                     /locus_tag="PK641_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55435.1"
                     /translation="MSKSIVLRLLTIVTIILMVSCEVTDPYTKNKINGVWLLESINGT
                     NVNTSERIVYEFNNDKTALFAMRYEESETYKSQWIEIKNYNWDLVSTNLQINGVDAKG
                     ETNAISAVLQTVNDTLLSFKASGRNLGDNNESTDNNLVFKRVLKQNYLYFGTWEMIQK
                     DDVSKTDVRITFNEDGTYIYSEYVDGNWVEKQDNNGQWFAYGSIIATNYTLNGEKICD
                     VWDFDTASSGQVNTWVWTMNNEDIKSSVIFRKVN"
     gene            complement(5331..6527)
                     /locus_tag="PK641_03440"
     CDS             complement(5331..6527)
                     /locus_tag="PK641_03440"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPG55436.1"
                     /translation="MNQVSPRLAVLSESETLAMSQKSNELKAQGIDVINLSVGEPDFN
                     TPDHIKAAAKKAIDDNFSFYSPVPGYPALRNAIVAKLKKENDLDYTAAQILCSNGAKQ
                     SVCNVLLCLIGKGDEVIVPAPYWVSYVEMVKLCDGTNVIVKTGIEQDFKMTPAQLEAA
                     ITPKTKALILCSPSNPTGSVYSKEELKGLADVLAKHPQVYIISDEIYEHINYVGKHES
                     IAQFDEIRDRVVIVNGVSKGYAMTGWRIGWIAAPLWITQACNKLQGQYTSGPCSIAQK
                     ASEAAYTGDQACVENMRMAFERRRDLIYNLIKPIPGLQINKPNGAFYVFPKCDSFFGK
                     STPNGKKINSAGELAMYLLEEGHVACVGGDAFGQPECIRISYATSDENIKKAIERIKN
                     ALAELK"
BASE COUNT         2147 a         1110 c         1198 g         2128 t
ORIGIN      
        1 atgattgcct cgacgccctc ggctcgatca ttggcgaaat atcttctgaa gaagtgcttc
       61 attcgatatt taaaaacttt tgtataggga aatagggtga tgtttctgtt gagataatgc
      121 gaagtataaa tgtgtcgaac gtaaatgcag aagatagtgt agggttttgt catttggcat
      181 tcgatatgga aaataaagat gcgctaattg caaaaatgat tgagttgaaa gcaaaaaatg
      241 ttcgtgttat tagtgatgca cgcgttacag gtgatggcta ttttgaaagc gtaatagccg
      301 atttcgatgg caataaaata gagttaagta ctacacaatt ttaaaattat ttttatgaaa
      361 gagttgaaat gtccaaaatg cggcaatgtt tttacagtag atgaggctga ttatgcttcg
      421 attgttaatc aggttaagaa tgctgaattt agcgaagaac taaatcttcg tgtagctgag
      481 ttaacgaagc agttagcaac tgaaaatgaa aaatctactc tcgaacttac taaaaatcac
      541 ttagctgaat taaacagaaa aaatgtagaa atatcaaagt tgtctgccga aaaagcaggt
      601 atagcaagtc ttactcaaaa tgagtttaac gaaaaattat ccgaaaaaga taaagagatt
      661 tcggcactta cagctaaagt aactgaaact caattaaaaa tacaacttgc tgtttatgaa
      721 gaacagaata aaaatatgtc tgagttgaat gacaaagata tgcagataga gaggctaaag
      781 atctgtatcg aaaatcaaaa acagaccgaa gaattagcaa aaacatcgct cgaaagaaag
      841 tatcaggaag agcttaagca aatgaaagag atgcttgaat attataagga tttaaaagca
      901 cgaatgtcaa ctaaaatggt gggtgaaacg ctcgaaagtc attgtaatgc agagtttggc
      961 cgattacgac aaatgtttcc gtatgcatat ttcgaaaaag ataatgatgc atcaagcgga
     1021 agtaaaggtg attttatttt tagagactat gaggatgaag agtcgatgaa gaggaagtct
     1081 gagtatgttt ccatcatgtt tgaaatgaaa aacgagatgg acgaaacagc aaccaaacat
     1141 aaaaatgaag attttttgaa aaagttagat gaagacagaa caacaaaaaa atgtgagttt
     1201 gctgtattag tgtcattact tgaacctgac agcgatctgt acaataccgg cattgttgat
     1261 atgtcacaca gatacccaaa aatgtatgtt atcagaccac aatttttctt gccgctgctt
     1321 accctgctga ttcaaacctc taagaaaagt atggagtata aaaaagcatt gacaatagct
     1381 caaagtcagt cggttgatgt gtctaatttt gaaaatcagc ttaacgactt taaagataag
     1441 tttggtaata attatcgttt agcaagcgag aaatttaaag ttgccataga tgagattgat
     1501 aaatcaatag accatctaca aaagattaaa agcaatcttt tgggctctga aaataatctt
     1561 cgcatagcta atgataaggc aagtaatctt acgattaaaa gattaacgcg tgataatgca
     1621 actatgaagg caaaatttga tgaagcgcgt aaaaatagcc caaatgcaat aatcgatgat
     1681 actgatactg tagattagta ttaaatcgat acccatttgt tcggtactct atgtagttac
     1741 cggactcaga ttgtgtatga gaaccacaaa tatcctaaca ttttaggatt gtagtttcat
     1801 ttttatagtg gcgtatacac agtggagtag tccctttaac ccccatagca gtaatgtaag
     1861 atggttaaat tatggcgatt cgcttgcagc tattcatcag taaaaaggta taaaaaatgc
     1921 ggtatacgtt ttcgtatacc gcgattaaat aattatgcaa gcctgatttt atttgatttc
     1981 tgtcttccac aatccatgca aattgcaata tgcatatact gcaactagtt tgtcatcaca
     2041 gagacatact tctgcacttg gttcacctgc atgatcaaga taaacaagtt tacctccgct
     2101 tgtggtttca aaatagagaa aatcaataaa atgttcgggt accattggat gaggagtgct
     2161 tccgattttt acattccaaa cgccatcact cactttagtt actacaggta aatgcttttc
     2221 ggttgatgca tctacagtat ttgcttgaag ttcttccatc tcttcatcgc agcataccat
     2281 tggaacacct gaactattaa caactgctac aagattgcca cagatgttgc atctaaaaaa
     2341 tttaacttgt tctttcatta tgaaataatt taatgttgtc ctacaaatat aagaattttg
     2401 atgagacttt atattttcat tatagaattt tacgaaaatg atttgttttt tttacgaaaa
     2461 caccaatgaa taagtattat agtaaggcca aaaatctata tacgaataga aaaaaacaaa
     2521 tgaacactaa tgaaggaagt aaatttacga ttttcagatt ctttattttg aggatattaa
     2581 tacctaagcc aattagcagt atgccaccaa cagatgttat tgctactata tattcgttag
     2641 gtatatcgcg agcaaataaa tacacgaata aagttagaat tccttgatag aaaagaacag
     2701 aaatagatgc aaataacaca cttgaaccaa atgcagatgc aagtagcata gccgaaaatc
     2761 catccaatat cgacttggtt aacaataggt ctgaagtctt tccaaatccc tcttcaatag
     2821 caccaattac cgacatagaa cccatgcaaa acagtagtgt agatgtaata aaaccttctg
     2881 taaattgttc gtttttagca tggagtctgc gttttaattt attgcttaga ttttctgtgc
     2941 gttcttctaa ttttaaatat tcgccacata aaccgccaca cgccaagcta agtactacta
     3001 ttaaaggatt attcataccg aaacccatct taacgccaag cataattgta aatatgccta
     3061 ctgcttgaaa gtatatattt acatattttt cagctaggtt ttttttgaaa atcattccaa
     3121 taaaaccacc tgcaataatt gctgcggtat ttataagtgt tccgatcata nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcagatagta agccggtttg tcgctatgcc
     3301 gcaaggtaga gaggaaagtc cgggcaacat agggtaccat gcttcataac gtgaagccgt
     3361 tcgtgagggc ggattagtgt aacagaaaat aaccgctgct atgcagtaag ggtgaaaagg
     3421 cgaggtaaga gctcaccgct tcggtcggaa tatcgaaggt cgtacacatc atgggttgca
     3481 aaaccaaata taccggcgta gtaggacgac ccgtctgatg ccgaggggta ggttgcttga
     3541 gcttgatggc aacatcaagt ccagataaat gacagacacc cgcaagggta cagaacccgg
     3601 cttataggct tactattttt tttgaaacat tatcatgaaa aaaataatag agtattgtaa
     3661 agaaatttac aaatttatca cggagaaaat ttggaaaatg cctgccgaac agcaagataa
     3721 aaacttcttg attaggctta ttatggttat atatcttgct attaccagat atataaaaga
     3781 tgagttggct gtaagagctt ctgcattaac atattataca atatttgctg ttgtcccatt
     3841 cctttgctta ataattggta taggaaaaca ttttggagtg caacattata tagagtcaga
     3901 gttgatactt atgtttcccg gtcaggaaga aatgttgata catgcatttg agattgttga
     3961 ttcatatttg atgcagatac atggaggatt tcttttagtt gcagttattt tattactttg
     4021 gactttgtat ggcgttgtaa ctcaggtaga agtagcattc aacaatatat ggcgtgttca
     4081 ccaaaataga aaaatatatt atcgtgtcgg aggccttttg cttatgatgg ttctgttccc
     4141 catatttctg ttcgtatcag gtgttgtatc aatatatatg aatacaacat tatcgtcact
     4201 aaatatcttt ttaaaaccgg tatggaataa tatattggag ttgtttcctt cgtttataaa
     4261 cgtaatattg ttattaatgc tatatctagt aatgcctaaa acaaaggtta gatttgtacc
     4321 ggcttttatt ggagctttat tagcaggtat tgtacttgct gtatttcaac aattatatat
     4381 tagcggacaa atttgggtag cccattataa cgctatatat ggaagtttcg cagctattcc
     4441 tttattactt ttgtggctac aattttcgtg gatgataata cttgtgggtg ctgaattatc
     4501 tcactgtata caaaatcgcc acaatctcgg taaggaaatt gtggcggata ataaataatt
     4561 gttgtttgtt ttaatttacc ttgcggaaga taacagatga ttttatgtct tcgttattca
     4621 tagtccaaac ccaagtatta acctgacccg agctagcagt atcaaaatcc catacatcac
     4681 atattttttc tccatttaaa gtataattgg tcgcaataat tgatccataa gcaaaccatt
     4741 ggccattatt atcttgtttc tcaacccaat taccatcaac atactctgag tatatatatg
     4801 tgccgtcttc attaaaagtt atacgaacat cggttttaga tacatcatct ttttgaatca
     4861 tctcccaagt tccaaaatat aagtagtttt gtttgagtac acgcttaaac actaggttgt
     4921 tgtcagtact ttcattgtta tctcccaagt ttcttcctga tgctttaaaa ctaagtagag
     4981 tgtcatttac cgtttgtaaa acggcagata tggcattagt ttcacccttg gcatctactc
     5041 cgtttatttg taagttggtg ctaaccaaat cccagttata atttttaatc tctatccatt
     5101 gagatttata agtttctgat tcttcgtagc gcattgcaaa taaagcagtt ttgtcattat
     5161 tgaattcgta tacaatgcgt tcggaagtat taacgtttgt tccattaatt gactctaaaa
     5221 gccatacacc attaatttta tttttggtgt acgggtctgt aacttcgcat gaaaccatca
     5281 atataatggt tacaatagtt agtaaacgga gtactattga tttactcata ttatttaagt
     5341 tctgctaaag cgttcttaat acgttcaatc gctttcttga tgttttcatc agaagtagca
     5401 tacgaaatac gtatacattc aggttgtccg aatgcgtcac ctccaacaca agcaacatgt
     5461 ccttcttcca gcaagtacat tgcaagttct ccggcactat ttattttttt accattggga
     5521 gtgctctttc caaagaatga atcgcatttt ggaaatacat aaaatgctcc gtttggttta
     5581 ttaatttgta agccgggtat tggtttgatt aaattgtaaa ttaaatccct acgacgttca
     5641 aatgccatac gcatattctc tacacatgct tgatctccgg tgtatgcagc ttctgaagct
     5701 ttttgagcaa tagaacaagg accggaagta tattgacctt gaagtttatt gcaggcttga
     5761 gtaatccaaa gtggtgcagc aatccaacca atacgccatc ctgtcattgc gtatccttta
     5821 gaaactccat ttacaataac aactctgtct cttatttcat caaattgagc tatgctttcg
     5881 tgcttgccaa cataattgat atgttcgtat atctcatcag atattatata tacctgtgga
     5941 tgttttgcta atacatcagc caagcctttc aattcttctt ttgaatatac acttcctgtc
     6001 gggttagatg gtgaacacaa aataagagct ttggtttttg gggtaattgc agcctcaagt
     6061 tgtgccggtg tcattttgaa atcttgttcg atgcctgttt ttacaattac gtttgtacca
     6121 tcacataatt ttaccatttc aacatagctt acccaatatg gtgcaggaac tataacttca
     6181 tctcctttac cgattaagca aagcaaaaca ttacaaacag attgttttgc accatttgag
     6241 catagtatct gtgctgcagt ataatccaaa tcattctcct ttttcaactt agcaacaatc
     6301 gcatttctca gtgcaggata tcccggaacc ggagaataga acgaaaaatt atcgtctatt
     6361 gctttttttg cagctgcttt aatgtgatca ggagtgttaa aatcaggctc tccaacactt
     6421 aaattgatga cgtcaattcc ttgtgcctta agttcgttac tcttttgcga catagctaat
     6481 gtttccgatt cggaaagcac tgccaaacgt ggtgaaactt ggttcatgat ttatatattt
     6541 atatatttat ctgtctttct ccttgcaaaa gtagtgattt ttttgtacct ttgcgatcaa
     6601 aatgatataa aatggaacta aaaaagtgca tttttgtttt ttttatgatg tattcggctt
     6661 gcgtaatggc agatggtgac gaa
//
LOCUS       DAOPID010000067         6670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_14803_length_6670_cov_3.898262, whole genome shotgun sequence.
ACCESSION   DAOPID010000067 DAOPID010000000
VERSION     DAOPID010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 6670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6670
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14803_length_6670_cov_3.898262"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..918)
                     /locus_tag="PK641_03445"
     CDS             complement(97..918)
                     /locus_tag="PK641_03445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HPG55437.1"
                     /translation="MIIFAVNNKVFININSSTMKKNLILLASMVALLVSCSSNKNEKV
                     VDIDGNEYTTIVIGNREWMVENLKTTRYNDGTPIQYCADDSAWIKAGETNEAAWCVYG
                     EYGSAQEAVASTTFGTYGVIYNFFAVEKDKLAPEGWRVATNNDWVALENYLVAQGFNY
                     DDTRSDNKIAKALTDNILWKQNTDDGLGDIGTKSSFVEKNNATGFSAVPGGDRRISGL
                     YVFLNEQTFIWTSTVCGTDSTAAWARQLSNTEYSLVTLPAKAQYGFSVRCVRDVK"
     gene            961..1611
                     /locus_tag="PK641_03450"
     CDS             961..1611
                     /locus_tag="PK641_03450"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPG55438.1"
                     /translation="MRKNKVFCLIFTLICITNAFSQEWHFGVTAGYENTGATWFQEGV
                     KKELRNISGFNAGPVVAYDFIDYFSLQSGLYFAMNGFETSSHNILWNKYDVVTSEKTI
                     LYYLQLPIFAMGKLPIGQVNFLLEAGPILSYGVASHTSTQIRVGNQTETYNSNDAFNE
                     SLYPFNCLIHLGAGAEISGARLIVGYNFGVFDIGRDTKNNNDMKTSGFVVSAAYLF"
     gene            1618..2847
                     /locus_tag="PK641_03455"
     CDS             1618..2847
                     /locus_tag="PK641_03455"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPG55439.1"
                     /translation="MKFDELNLEDALLDGLCAMNFKETTPIQEKTIPIILEGKDMIAC
                     AQTGTGKTAAYLLPILNILCKGEHREDKINALVMVPTRELAQQIDQQLEAFAYFLPVS
                     SVAVYGGGDGGLFDQQDKALRSGVDVVIATPGRLIALLNMGRIDMSFVKFFVLDEADR
                     MLEMGFVDDIMRIASYLPEKKQTLLFSATMAPKILTLAKKTLINPVEVKLSVSKPAEK
                     ISQSAYVCYETQKMPIIESIFKNKSPERVIIFSSSKQKVKDIYLMLRRMNFKVAAMHS
                     DLEQKEREDVLRQFKNGSIKLLVATDIVARGIDIEDIEMVINFDVPHDVEDYLHRIGR
                     TARANADGEAITFISDKEQYKFKAIENFIEKDVKKIPVPEEIGATPLYNPIKQEGDGR
                     KNFYRKYKNNRPKRNTN"
     gene            2942..4252
                     /locus_tag="PK641_03460"
     CDS             2942..4252
                     /locus_tag="PK641_03460"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HPG55440.1"
                     /translation="MPKIIIIGGGIAGLSAGIYARKCGFECEIYEKHVRVGGECTAWE
                     RKGYHIDNCVHWMTGTSPSSEIYETWCAVHALGKDVEIIQNPSFLRVEYNNQKLELWQ
                     DLNRLKQDMLAISSEDSIEIERFIENIRLYQSVILPAHKPLEQYSIWEVFSLIKSMFA
                     VGRIHKEYSRTSLKTYSQRFKHPLLQKMILSYMPDKYNIASFFYVLGTFTSGNGAIPK
                     GGSTGIQNRMPALYTELGGITFCRKEAIGIDIEKSQAKRVHFKDGTSAEGDFIIFACD
                     ASVVFEKILGEKHIDKYFKSHFSQKIKYPIYSSFNAYIGVDESKLDISDTTWFEGKEF
                     DICGKKCNSFLLKYYGIEPGFSPEGKSLIKALVVQYEDDFEYWKSLHSKSINEYREEK
                     LRVAQSIISNIEDHYPELKGKLEVVETVTPMSFIGCVVHTKGHI"
     gene            4249..4434
                     /locus_tag="PK641_03465"
     CDS             4249..4434
                     /locus_tag="PK641_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55441.1"
                     /translation="MSFILTPFAKKKIHKGRLKHIKNVYLAGQWIQPPGGLPNAVVTG
                     KFAIQRICKKEGFKFVQ"
     gene            complement(4502..5818)
                     /locus_tag="PK641_03470"
     CDS             complement(4502..5818)
                     /locus_tag="PK641_03470"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPG55442.1"
                     /translation="MKAKCVILFLLLPLITSAQQVWTIEQCIEYAKNENAVIKRSETQ
                     AEISKISHQQSKENFLPNLNANASQNFSFGRSLTEDNIYMSNNVFNTNIGVSAGLTLF
                     KGLQMINEVYQTDFNFQAALMDVKKQQDDICLQIMTCYLQILFNKEAVKVAESKLELT
                     KLQAKQTEILVEAGKKPKGELSEANALVAQDKVSLLDAKTTLETSYLTLTQLLYLGNE
                     DVKNFDVSDDVSFMMTDVLNKSVSEIYDAALEFLPSIKGEELRIKSQEYSVKMAKGAY
                     SPQLSFGASYGNGYYYTQGGANLSFADQFAKNNNFGLNFSLYIPIFDRMSTPNAIKRS
                     KLTLDQQSDQLETAKNNLRKEIQNAYVNAYMAQQKIQLAEANLQASEEAFNYQSEKYE
                     AGAATLYELNESKNRYTSAKLSQVQAKFNYLFRTRILSYYNGELRF"
     gene            complement(5823..>6670)
                     /locus_tag="PK641_03475"
     CDS             complement(5823..>6670)
                     /locus_tag="PK641_03475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPG55443.1"
                     /translation="GEIVKKGDIIAKVRVVPEMGALNNAETNLKKSKIALEKAQIDYN
                     RQETLYKKGVVSQSDFEDTEVNLKNAKENLIDAQNSYDIIKDGITKNSANSSNTLIRA
                     TVDGTILDVPEKVGNSVIQSNTFNDGTTIATIANMGDMLFVGKIDETEVGKLKPGMPI
                     KLLIGALEGRMFDANLEYIAPKGTEENGAVMFEIKAAVKIPDDVFIRAGYSANAQIVL
                     AKSDSVMSIPESALKFSGDSTYVEVRSTKDTLLFEKRPVVIGLSDGLNIEIKQGLKMK
                     DIIKK"
BASE COUNT         2177 a         1183 c         1124 g         2186 t
ORIGIN      
        1 ttgcttttag tgaatctttt aacttcatgt tgatttatat tataaaaaat agggccgcaa
       61 gtaagatgcg accctacata ttgagatcag tgaaacttat ttcacatcac gcacgcagcg
      121 tacgctgaaa ccatattgag cttttgccgg tagtgttacc aaagaatact ccgtattact
      181 caattgacgt gcccatgcag ctgttgaatc agtaccgcac actgtagatg tccaaataaa
      241 tgtttgttcg ttcaagaata cgtatagtcc gctaattcta cgatctccac caggtactgc
      301 tgaaaatcct gttgcgttat ttttctcaac aaaacttgat tttgtaccta tatcacctag
      361 tccatcatct gtattttgtt tccacaaaat attatcggta agtgctttag ctattttatt
      421 atcactacgt gtatcgtcat aattaaagcc ttgtgcaacc aaatagtttt ctaatgctac
      481 ccaatcatta tttgttgcaa ctctccaacc ttccggagct aatttatctt tttctacagc
      541 aaagaagtta tatataacgc cgtaggttcc aaatgtggtt gacgctacag cctcctgtgc
      601 cgaaccatat tcaccatata cacaccatgc agcttcattt gtttcacctg cttttatcca
      661 tgcactatca tcagcacaat attgaatagg agtgccatca ttataccttg ttgtttttaa
      721 gttttcaacc atccattcgc ggttgcctat tacaattgtt gtatattcgt taccatcaat
      781 atcaacaact ttctcatttt tattagatga acaagacacc aacaatgcaa ccattgatgc
      841 caacaaaata aggtttttct tcatagtact tgaattaata tttataaata ctttgttatt
      901 gactgcaaaa ataatcattt ttgcaaaaaa aacgtatctt tgtacgaaaa tatatctaca
      961 atgaggaaga ataaagtctt ttgcctaata tttaccttaa tttgcattac aaacgcattc
     1021 tctcaagaat ggcattttgg agtgactgcc ggttacgaaa atacaggtgc aacgtggttt
     1081 caagaagggg taaaaaaaga attacgaaac ataagcggat ttaatgcagg tccggtggta
     1141 gcatacgact ttatagatta tttttcatta caatccggat tatactttgc aatgaatggt
     1201 tttgaaacta gtagtcataa tatattatgg aacaaatacg atgtagtaac aagtgaaaaa
     1261 actatactgt actatttaca acttcctatt tttgcaatgg gtaaattacc tataggtcaa
     1321 gtaaattttt tattggaagc cggtccaatt ctttcatatg gggttgcgag tcatacttca
     1381 actcagataa gagtaggaaa tcaaacagaa acatacaata gtaatgatgc atttaacgaa
     1441 tcattatacc cttttaactg cttaattcat ttaggagccg gtgcagaaat ttcaggtgca
     1501 agacttattg taggatataa ctttggtgta tttgacattg gtcgcgacac taagaacaac
     1561 aacgacatga aaactagcgg atttgttgta tctgctgcat atttatttta actgactgtg
     1621 aaatttgatg aattgaattt ggaagacgca ctattggatg gtttgtgcgc catgaatttc
     1681 aaagagacca caccaatcca agaaaaaacg attcctatta tacttgaagg taaagacatg
     1741 attgcctgcg cacaaaccgg tacaggcaaa actgcagcat acttattacc tattctgaat
     1801 attctatgta agggcgaaca cagagaagac aaaattaatg cccttgtaat ggttcctaca
     1861 cgcgaattag ctcaacaaat tgatcaacaa cttgaagcat ttgcatattt cttgcctgta
     1921 agctctgtgg ctgtatatgg tggcggagat ggagggctat ttgaccaaca agacaaagca
     1981 ttaagatccg gagttgacgt agtaattgca acacccggac gcctgattgc attactcaat
     2041 atgggtagaa ttgatatgtc tttcgttaaa ttctttgtct tagatgaagc tgaccgcatg
     2101 ctagaaatgg gtttcgttga tgacattatg cgcatagcat catatcttcc cgagaagaaa
     2161 caaactttgc ttttctcggc aaccatggct cctaagatat tgacgttggc aaagaaaact
     2221 cttatcaatc cggtagaagt aaaattatct gtatcaaaac ctgcagaaaa gattagtcaa
     2281 tctgcatacg tctgctacga gacacaaaaa atgccgatta ttgaatcaat tttcaaaaac
     2341 aaatctcctg aacgcgtaat aatattttct tcatcaaagc aaaaagtaaa ggatatatat
     2401 ctgatgctac gcaggatgaa ttttaaggtt gctgcgatgc attccgattt agagcaaaaa
     2461 gagcgtgaag atgttttgcg tcaatttaag aatggaagca tcaaattatt agttgcaaca
     2521 gatattgtgg ctagaggtat agacattgaa gatattgaaa tggttattaa tttcgatgtt
     2581 ccgcatgatg ttgaagacta tttacacaga attggacgta cggcacgtgc aaatgcagac
     2641 ggcgaagcta taacatttat atcggataaa gaacaatata aatttaaagc tatagagaac
     2701 ttcatcgaaa aagatgtaaa aaaaatacct gttccggaag aaattggagc cactcctcta
     2761 tataatccaa tcaagcaaga aggagacgga agaaaaaatt tttataggaa atacaaaaac
     2821 aatcgtccca aaagaaatac gaattaagta tttaagaact ctttaaatgc gttttataca
     2881 tatttatgag taattttaca aaaaccgtaa gcaaacatta taagagaaat tttacatatt
     2941 tatgcctaaa ataataatta taggaggcgg catcgccggt ttatcagccg ggatatacgc
     3001 acgaaaatgt ggttttgaat gcgaaatcta cgagaagcat gttagagttg gtggagagtg
     3061 caccgcttgg gaacgcaaag gttaccatat tgacaattgc gtacattgga tgaccggtac
     3121 atcgccaagt agtgagattt atgaaacatg gtgtgcggta catgcactcg gaaaagatgt
     3181 tgagataatt cagaatccat cttttttaag agttgaatat aataatcaga agctggaatt
     3241 atggcaagat ttgaatcgac taaaacaaga tatgctagcc atatcttcgg aagattctat
     3301 agaaattgag agattcattg aaaatataag actataccaa tctgttatac tacctgctca
     3361 taaacctttg gaacagtatt cgatatggga agttttttct ttaataaaaa gtatgttcgc
     3421 agttgggcgt attcataagg aatattctcg cacatcattg aaaacatatt ctcagagatt
     3481 caaacaccct cttttgcaaa agatgatact atcttacatg cctgataagt acaatatagc
     3541 atcatttttt tatgtacttg gaacatttac atccggtaac ggagcaatac caaaaggcgg
     3601 ttcaacaggc atacaaaaca gaatgcctgc cctatacaca gaacttggtg gtataacttt
     3661 ttgtagaaaa gaagcaatag gtattgatat agaaaaatcc caggctaaaa gagtacattt
     3721 taaagatggc acgagcgcag aaggcgattt tattatattc gcttgcgatg catcggtagt
     3781 ttttgaaaag atacttggtg aaaaacatat agataaatac ttcaaatctc atttttctca
     3841 aaaaatcaaa tatcctattt actctagttt taatgcttat attggggtag atgaaagtaa
     3901 attagatata tctgacacta cttggttcga aggaaaagaa ttcgatatat gtggcaaaaa
     3961 atgtaattcg ttcctgctta agtattatgg tatcgaaccc ggattttcgc ccgaaggcaa
     4021 gtcattgata aaagctttag tagtccaata cgaagatgat ttcgaatatt ggaaaagttt
     4081 acacagcaaa agtattaatg aatatagaga ggaaaaacta cgagtagcac aatctataat
     4141 ttcgaatatt gaagatcact atcccgaatt aaaaggtaaa cttgaagttg tagagactgt
     4201 tacacctatg agttttatag gatgtgtggt gcatacaaag gggcatatat gagttttatt
     4261 ctaaccccat ttgcaaagaa aaaaatccac aaaggccgct taaaacacat aaaaaacgta
     4321 tacttggcag gtcagtggat tcaaccaccc ggaggattac caaacgcagt tgttacaggt
     4381 aagttcgcaa ttcagcgaat ctgcaaaaaa gaaggcttta aatttgtaca ataaatttct
     4441 aataaagaag gggccatatc aagtgatatg accccttctt tattgcatgt aatcaaacta
     4501 tttaaaatct aagttcgcca ttgtaatatg ataaaattcg agttctgaat aaataattaa
     4561 attttgcttg aacctgcgat aattttgctg atgtgtaacg atttttagat tcattcaatt
     4621 cgtacaatgt tgctgcacct gcttcgtatt tttctgactg ataattaaat gcttcttctg
     4681 atgcttgtaa gtttgcttct gccaactgaa ttttttgttg agccatatat gcattaacgt
     4741 aagcattctg aatctcttta cgtaaattgt ttttagctgt ttcaagttga tcagattgtt
     4801 gatctaatgt caatttgctg cgttttattg catttggagt actcattcta tcaaagatag
     4861 gaatataaag actgaagttc aatccgaaat tattattctt ggcaaattga tcagcaaaac
     4921 ttaaatttgc tccaccttgt gtatagtaat atccgtttcc ataactagca ccaaaactca
     4981 attgaggaga ataagctcct ttagccatct tcacagaata ttcttgactt ttaattctca
     5041 attcttcacc tttaattgat ggtaaaaatt ctaatgctgc atcatatatt tctgaaactg
     5101 acttatttaa aacatctgtc atcataaaac tcacatcatc cgaaacatca aagttcttta
     5161 cgtcttcatt tcctaaatat aatagctgag ttaaagtcaa atatgatgtc tctaaggtag
     5221 tttttgcatc taacaaagaa accttgtctt gagcaactaa agcatttgct tcggatagtt
     5281 ctcctttagg ttttttccct gcttcaacca atatttctgt ttgtttagcc tgcaattttg
     5341 tcaattcaag cttagactct gcgactttta ctgcctcttt gttgaacaaa atctgtaaat
     5401 agcaagtcat tatctgcaaa caaatatcgt cttgttgttt ctttacatcc ataagagctg
     5461 cctgaaaatt aaaatcagtt tgataaacct catttatcat ttgcaaaccc ttaaaaaggg
     5521 ttagtccggc agatactcct atatttgtat tgaaaacatt atttgacata tatatattat
     5581 cttctgttaa tgaacgtcca aaactaaaat tctgtgacgc atttgcattc aaatttggaa
     5641 gaaagttttc ttttgattgt tgatgcgaaa ttttagaaat ctcagcctgt gtttctgacc
     5701 tcttaattac cgcattctcg ttttttgcat attcaataca ttgttcaatt gtccaaactt
     5761 gttgtgctga tgttattaat ggaagcaata aaaataatat tacgcattta gctttcataa
     5821 atttattttt taattatatc cttcattttt aaaccttgct taatttcaat attaaggccg
     5881 tctgatagcc ctataacaac cggacgcttt tcaaacaata aggtgtcttt tgttgaacga
     5941 acttcaacgt atgtgctgtc acctgaaaat ttcaatgcac tctcaggtat tgacataacg
     6001 ctgtcacttt tagcaagaac aatttgtgca tttgcactat accctgctct tataaaaaca
     6061 tcatcaggaa tcttcacagc tgccttaatt tcaaacatta cagcaccatt ttcttcggtc
     6121 cctttaggtg caatatattc caaatttgca tcaaacatac gcccttcaag ggcacctatc
     6181 aacaatttaa taggcatacc gggttttaat tttccgactt ccgtttcatc tatcttacct
     6241 acaaacaaca tatcccccat attggctatt gttgctattg ttgtaccatc attaaatgta
     6301 ttactttgaa ttacagagtt accaactttt tcaggcacgt ctaagattgt tccatcgact
     6361 gttgcgcgta ttaatgtatt actgctgttg gcagaatttt tggttatacc atcttttatg
     6421 atgtcgtagc tattttgtgc atcaatcaaa ttttctttgg cattttttag atttacttcg
     6481 gtatcttcaa aatcagattg cgaaactaca ccttttttat ataatgtttc ttgacgatta
     6541 tagtcaattt gagccttttc taaagctatt tttgatttct ttaaattggt ttcagcatta
     6601 ttaagtgccc ccatttcagg aactactcta actttagcga taatgtcacc ttttttcact
     6661 atttcaccgg
//
LOCUS       DAOPID010000068         6652 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_14864_length_6652_cov_4.354404, whole genome shotgun sequence.
ACCESSION   DAOPID010000068 DAOPID010000000
VERSION     DAOPID010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 6652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6652
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14864_length_6652_cov_4.354404"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="PK641_03480"
     CDS             <1..1024
                     /locus_tag="PK641_03480"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HPG55444.1"
                     /translation="EKRTRFWMVIINLQNYIMKDYIKLIRWPNLLFLGALNALVYYMV
                     VCPILNMYSIKLDNELWIFILLIISNIFIAAGGYVTNDYFDTKIDEINRPTKVIVGKT
                     KTRKQVAVYFQILFGIGIIAGIILSILLKDYTLAFVYITIPGLLWFYSSSYKRQFLIG
                     NIVVGICTFLVIFIIGYAANTALFNQYGELIYQTNIITEIYKWTASIGLFAFLLTLIR
                     EIVKDMEDIEGDREMECRTMPIVWGINKSKIVVAVLSTITLLIVAYFTFYKIPFENDP
                     VSIKYFIFGIVLPFVVMFYLLGKAQSRRDFAQISLLLKYIMAVGSLYCVFIYYLILLN
                     TTSICS"
     gene            1015..1605
                     /locus_tag="PK641_03485"
     CDS             1015..1605
                     /locus_tag="PK641_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HPG55445.1"
                     /translation="MLLTEKLKEYELVLVSQSPRRRELLEGLEIDFSSTSVDIYETYP
                     ETLSGAQIPMYIAEAKASAYEPKLNNSTIAITADTVVLFEDDILEKPENAEKAKDMLR
                     RLSGNTHKVITGVCIFNKNKKRSFHAISDVTFATLTDEEIDHYVEVYNPFDKAGSYGV
                     QEWIGYIGIEKIVGSYYNVMGLPVHRLYKELNAFFG"
     gene            1605..2834
                     /locus_tag="PK641_03490"
     CDS             1605..2834
                     /locus_tag="PK641_03490"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPG55446.1"
                     /translation="MEINKHSFSNGLRLIHSFTDITQNVTINILYGAGSRNEKSGKTG
                     IAHLLEHIMFCGSQNIPNFDQPLEAIGGTSNAFTNNDITNYYLTIPKEAAELGFWLES
                     DRMLNPTLSQNSFDTQKKIVLEEFKQNLNEPYGDAHSLLRKLCYKQHPYKWSTIGQSL
                     DEVENLELKDLLSFHKRNYVPANAVLSITGNISYEDALSLTEKWFAGIPYSVYVEDNI
                     PDEPEQTSTRFETYYRNIPQNAIYKAYHICNVKNKDIFKYDLLSDILANGKSSRLINN
                     LVYEKKLFTSIDAFIENSFDTGLLRIVGKIQDNVSPKEADAAINEETEKLRSQIVSDY
                     EMEKWKNKYESTQLAERFNIEHLAEMLAFYEYIGQTERFLNNIDFYNSVSASDILTIS
                     NNLFDSKHSNTIWYLKN"
     gene            2908..4113
                     /locus_tag="PK641_03495"
     CDS             2908..4113
                     /locus_tag="PK641_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPG55447.1"
                     /translation="MPQTSERGKMMPQSPIRKLVPLADKAKERGVKVYHLNIGQPDLN
                     TPQVALEAIRNIDRKILEYSPSDGIKSLRVKLAEYYKRFKINVEPSDIIVTSGGSEAV
                     NFAFMSCLDPGDEIIVPEPAYANYTAFAISAGAVIKPIVSSIEDGFALPSIDKFEEQI
                     TPRTKGILICNPNNPTGYLYTQNEMDRIRDLVKKYDLFLFSDEVYREFCYTGAPYISA
                     FHLKGIEENVVLFDSVSKRYSECGLRIGALVCKNKAVKESVMKFCQARLSPPLIGQIA
                     AEASVDTPPEYMLEMYNEFVERRKFLIDGLNRIPGVYSPIPMGAFYTVARLPIDDSDK
                     FCKWLLEEFEYEGQTVMMAPASGFYTMNDVGRNEVRVAYVIKKEDLAKALFVLRKALE
                     VYPGRTHTS"
     gene            4113..5366
                     /locus_tag="PK641_03500"
     CDS             4113..5366
                     /locus_tag="PK641_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG55448.1"
                     /translation="MNLELFIARKIHFQTPAKKSKSNITKPAVRIAIAGIALGIAVMI
                     ISVGVITGFKHEIREKLIGFSSHIQITSLTNNNTFEMAPISFSATLVDSIKQIPEVRN
                     TCFYCTKPAIIKTSNDFQGLAFNGVDNNYDWTFFKNNLKEGRLPDFSDTLPSKEILIS
                     RKIASMLNISVGDGILTYFIQSNVRVRKFTVSGIYETGLQDFDKMFMIGDLRQVQSLN
                     GWNSDEVGGMNIWVKDFNKSEETAYEIFETTANKFEQHGSTYYTRTIRELQPQIFGWL
                     ALLDTNVIVILILMMLVAGFNMISGLLILILDKTNMIGVLKSIGLRDWSIRKIFLYQS
                     CFLIGKGLLLGNIFGICICLIQEYFKIIPLDSSIYYVDTVPIILQPTAIIGLNIMAAI
                     VSLLMLLIPSHVIAKISPAKSIRFE"
     gene            complement(5367..6569)
                     /locus_tag="PK641_03505"
     CDS             complement(5367..6569)
                     /locus_tag="PK641_03505"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HPG55449.1"
                     /translation="MEYQDVLNYLYEKTPMFQKIGAGAYKEGLDNVKFLDEQYGHPHK
                     AYPTIHVAGTNGKGSVSHTIASVLQAAGYKVGLYTSPHLKDFSERIRINGTQISHDYV
                     VDFTERAMNLIESQKPSFFEFTTMMAFCYFKDNNVDVAVIEVGLGGRLDSTNIITPIL
                     SIITNISFDHIKQLGNTIESIAGEKAGIIKPKVPVVVGEAPVDLRPIYDQKGSPVVYA
                     EDFEYPPIDFELKGFCQEKNKKTIRAALEVLKLQFDITDDNIKEGFAKVVENTGLCGR
                     WQKIGDSPTIMIDTAHNEGGIRYVAEQLLAQKCSVLRIVFGMVNDKDINAVLALMPKK
                     AKYYFTNASISRALPAADLQKSAMQYDLKGDCYSTVNDAYKQACADASPDDFIYIGGS
                     NFVVAEVM"
BASE COUNT         2276 a         1126 c         1123 g         2127 t
ORIGIN      
        1 tgaaaaacga acacgctttt ggatggtaat aataaactta caaaactata tcatgaaaga
       61 ttatattaaa ttaataagat ggcctaattt attgttttta ggagcattga atgcacttgt
      121 atactacatg gtagtatgcc ctattctaaa tatgtattca attaagctgg ataacgaatt
      181 gtggatattt atattgctga taatatcaaa catatttatt gcagcaggag gctatgttac
      241 caacgactat tttgacacaa aaattgatga aattaacaga cctacaaaag ttattgttgg
      301 taaaacaaaa acaagaaaac aggtagctgt ttactttcaa atattatttg gcataggtat
      361 tattgccgga attattttgt caatattatt aaaagactat acattagctt ttgtttatat
      421 tactattccc ggattattat ggttctattc gtctagctac aagcgtcaat ttttaattgg
      481 caatattgtg gtcggaattt gtaccttctt ggtaattttt attattggtt atgccgcaaa
      541 tacagctcta tttaatcaat atggtgaact tatataccaa acaaacatca ttactgaaat
      601 atataaatgg actgcaagta ttggtttatt cgcattttta ctaacattaa ttcgtgagat
      661 tgtaaaagat atggaagaca tcgaaggtga tagagaaatg gaatgtagaa ccatgccaat
      721 tgtatgggga ataaacaaat caaaaatcgt tgtagctgta ttgtctacaa taactcttct
      781 tattgttgca tattttactt tttacaaaat accgtttgaa aacgaccctg ttagtattaa
      841 gtatttcatt tttggaatag tattaccatt tgtggtaatg ttttaccttt taggtaaggc
      901 acaaagtcgt cgtgattttg ctcaaataag tttattatta aagtacatta tggctgtagg
      961 tagcctatat tgtgtattta tttattatct aattctatta aacacaacat cgatatgctc
     1021 ttaactgaaa aattaaaaga atacgaatta gtattagttt cacagtctcc acgcagaaga
     1081 gaattactag aaggattgga aattgatttt agctctactt ctgtagacat atatgaaaca
     1141 tatcctgaaa cgctttcagg cgcacaaatt cctatgtaca tagccgaagc aaaggcaagc
     1201 gcttatgaac caaaattaaa taacagtaca atcgcaatta cggcagatac tgtcgtttta
     1261 tttgaagacg acatactaga aaaaccggaa aatgctgaaa aagcaaaaga tatgttgcgt
     1321 cgtctttcag gcaatacaca taaagtaata accggtgttt gcatatttaa taagaacaaa
     1381 aagcgtagtt ttcatgccat atcagatgtt acatttgcta ctttaacaga cgaagagatt
     1441 gatcattacg tagaagttta taaccctttt gataaagcag gctcatatgg tgtgcaagaa
     1501 tggattggtt acattggcat cgagaaaata gttggttctt attataacgt aatgggctta
     1561 cctgttcatc gtttatacaa agagttaaac gcattctttg gataatggaa atcaacaagc
     1621 actcatttag taatggctta cgtttgatac acagttttac tgatatcaca caaaacgtaa
     1681 caataaacat tctttatggt gcaggctcac gaaatgaaaa atcaggtaaa acaggcatag
     1741 ctcatttgtt ggagcatata atgttttgcg gttctcaaaa tattccaaat tttgatcagc
     1801 ctcttgaagc cattggtgga acttccaatg cgtttacaaa taatgatatc accaattatt
     1861 atcttacaat accaaaagaa gctgctgaat taggattttg gttagaatcc gataggatgc
     1921 ttaaccctac cttaagccaa aatagctttg atactcaaaa gaaaatcgta ttagaagagt
     1981 ttaaacagaa tcttaatgaa ccttacgggg atgctcattc attattgcga aaattatgtt
     2041 ataagcagca tccatataaa tggagtacaa ttggacagag tcttgacgaa gtcgaaaact
     2101 tagaattaaa agacctttta tcttttcata aacgcaatta tgttccggca aatgcagtac
     2161 taagcatcac cggaaacata agctatgaag atgcattatc actgacagaa aaatggtttg
     2221 ccggtattcc ttatagtgtt tacgtcgaag ataatattcc cgatgaacct gaacagactt
     2281 ctacccgatt cgaaacctat tatcgtaata ttccacaaaa tgccatatac aaagcatatc
     2341 atatatgcaa tgtgaaaaac aaagacatct ttaaatacga tttactatct gatattcttg
     2401 caaatggtaa atcatccaga ctaataaata atttagttta cgagaaaaaa ctttttacct
     2461 ctatagatgc ttttatagaa aattcttttg acacaggctt gttacgtata gttggtaaaa
     2521 ttcaagataa tgtgtcgccc aaagaagctg acgcagcaat taatgaagaa accgaaaaac
     2581 tacgttcgca aattgtgtcg gattatgaaa tggaaaaatg gaaaaacaaa tatgaatcta
     2641 ctcaattggc cgaaagattt aatattgaac atttagcaga aatgcttgca ttctacgaat
     2701 atataggcca aacagagagg tttttaaaca atattgattt ttataatagc gtatctgctt
     2761 ccgacatatt aacgatatcc aacaatttat ttgattctaa acacagtaat acaatctggt
     2821 atttaaaaaa ttgatgttct ttatcgtctt atatttcgat taaatcttta actttgcaat
     2881 tcgttcgaaa cataattatt tatcgcaatg ccacaaacct cagaacgcgg taagatgatg
     2941 ccgcaatcac ctatacgtaa attagttcca ttagctgata aagctaaaga aagaggtgta
     3001 aaagtatatc accttaatat tggacaaccg gatttaaata ctcctcaagt tgcacttgaa
     3061 gcgatacgca atatcgatcg aaaaattttg gaatatagtc cgagtgatgg catcaaatca
     3121 ttacgcgtaa aattagcaga atattacaaa cgctttaaaa taaacgttga acctagtgat
     3181 attattgtta ctagcggtgg atctgaggcc gtaaactttg catttatgtc gtgtctggat
     3241 ccgggcgacg aaattattgt tccggaacct gcatacgcaa actacactgc atttgcaata
     3301 agtgcaggcg cagtaatcaa acctattgtt tcaagcatag aagatggttt cgcattaccc
     3361 agcatcgaca aatttgaaga acaaattaca cctagaacaa agggtattct aatttgcaat
     3421 ccaaataacc caacaggata tttatatact caaaacgaga tggatagaat tcgtgattta
     3481 gtaaagaaat acgatttatt tctattctct gacgaagtat atcgtgaatt ctgctatacc
     3541 ggtgcaccgt atatttctgc ttttcactta aaaggaatcg aagaaaacgt agtgctattt
     3601 gattctgtat caaaaagata tagcgaatgc ggattaagaa ttggagcatt ggtttgtaaa
     3661 aataaggctg taaaagaaag cgtaatgaaa ttctgtcaag ctcgtttgag tccaccactt
     3721 ataggtcaaa tagcagcaga agcatcagta gatactccac cggaatatat gcttgaaatg
     3781 tacaatgaat ttgttgaaag aagaaagttc ttgatagacg gacttaatcg tattccggga
     3841 gtatattctc caattccaat gggcgcattc tacactgtag cacgtttacc gattgatgat
     3901 tcagataaat tctgtaaatg gttacttgaa gaattcgaat atgaaggtca gactgtcatg
     3961 atggctcctg cttcaggatt ctacaccatg aatgatgttg gtcgtaacga ggtgcgtgtt
     4021 gcatatgtta taaaaaaaga agatctagcc aaagcattat tcgtactacg taaagcattg
     4081 gaagtttatc ccggcaggac acataccagc taatgaatct tgaactattc atagcacgca
     4141 agattcactt tcaaacacct gcaaagaaaa gtaaatcaaa tatcacaaag ccagctgttc
     4201 gaatcgcaat agctggcatt gctttaggta ttgcagtaat gattatatcg gttggagtaa
     4261 tcacaggttt taagcatgaa atacgcgaaa agttaattgg ctttagttct catatacaaa
     4321 ttaccagcct aacaaacaac aatacattcg agatggctcc catctcattt tctgcaacat
     4381 tagttgattc gataaagcaa attccggaag ttagaaacac atgcttctat tgcacaaaac
     4441 ctgcaattat taaaacgtct aacgatttcc aaggactggc ttttaatggc gttgacaaca
     4501 attacgactg gacttttttt aaaaacaatc tgaaagaagg acgtttacct gatttctccg
     4561 acactttacc ttcaaaagag atccttatat cacgcaaaat tgcttcaatg cttaatatta
     4621 gcgttggcga cggaatcctg acatatttca ttcaaagcaa tgtgagagtt cgtaagttca
     4681 ctgtaagcgg aatctacgaa acaggtttac aagattttga caagatgttt atgattggag
     4741 atttaagaca agtccaaagt ttaaacggat ggaactcgga tgaagttgga ggcatgaata
     4801 tatgggtgaa ggattttaat aaatcggaag aaacggctta tgagattttt gaaaccaccg
     4861 ctaataaatt tgaacaacac ggcagtacat attatacaag aacaatacgc gaattacaac
     4921 ctcagatttt tggttggtta gccctactcg acactaatgt tattgttatt ttaatactaa
     4981 tgatgttggt agccggtttt aatatgattt cgggtttact aattcttatt ttagataaaa
     5041 ccaacatgat tggagtatta aaatcaatag gactaagaga ttggagtatt cgaaaaatat
     5101 tcctttatca atcatgtttt ttaataggga aaggcttatt gttaggcaat atctttggta
     5161 tctgtatatg cttaatacaa gagtatttta aaataatacc attagattca tctatatatt
     5221 acgttgatac agtgccaatt atcttacaac cgactgctat cataggatta aatattatgg
     5281 cagccatcgt ttcattacta atgttactga taccatctca tgtcattgcc aaaataagtc
     5341 cggctaaatc aattcgtttc gaatagctac atcacttcgg caaccacaaa attactaccg
     5401 cctatataaa taaaatcatc gggtgatgca tcagcacaag cctgcttata tgcatcattc
     5461 acagtagaat agcaatctcc ttttaagtca tattgcatgg ctgatttttg caaatcagct
     5521 gccggtaaag ctctcgaaat tgatgcgttg gtaaaatagt attttgcttt tttaggcatc
     5581 aatgccagta cggcattaat atccttatca ttcaccatac cgaagacaat tctcagtaca
     5641 gagcattttt gagccaataa ttgttctgca acatagcgaa ttccaccctc attatgagca
     5701 gtatcaatca taatagtagg actatctcct attttctgcc atcttccgca tagaccggta
     5761 ttttccacaa ctttagcaaa accttcttta atattatcgt cagtaatatc gaattgtaat
     5821 tttaaaactt ctaaggctgc tcgtattgtt ttcttatttt tttcttgaca aaagcctttt
     5881 aattcaaaat ctattggggg atattcaaaa tcttcagcat atacaacagg agaacctttt
     5941 tgatcatata taggccgcaa atcaaccgga gcctcaccta ctacaaccgg caccttaggt
     6001 tttataatac ctgccttttc gcccgcaata gactcaatag tattacctaa ttgctttatg
     6061 tgatcaaaac tgatgtttgt aataatgctt aaaataggcg ttataatatt tgtactatcc
     6121 aaacgaccac ctaaaccaac ttcaataaca gctacatcaa cattattatc tttgaaataa
     6181 caaaacgcca tcattgtagt aaattcaaaa aatgaaggtt tttgtgattc tataagattc
     6241 atggcacgtt ctgtaaaatc aactacataa tcgtgagata tttgagttcc gttgatgcgt
     6301 attctttccg aaaaatcttt tagatgtggt gatgtatata atccaacttt atatccggct
     6361 gcttgtaaca ctgatgctat ggtatgtgaa accgatcctt taccatttgt acctgctaca
     6421 tgtatggtag gatatgcttt atgaggatgt ccgtattgtt catctaagaa cttcacatta
     6481 tctaatcctt ctttgtacgc accggctcct attttttgaa acataggagt tttttcgtac
     6541 aagtaattta aaacgtcttg atattccatt attgaaaaat aatttgctta caaatgtact
     6601 tattctatta ttttattagt aaatttacgt cgaaaacttt aatcaaaatc gc
//
LOCUS       DAOPID010000069         6650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_14876_length_6650_cov_3.731312, whole genome shotgun sequence.
ACCESSION   DAOPID010000069 DAOPID010000000
VERSION     DAOPID010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 6650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6650
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14876_length_6650_cov_3.731312"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PK641_03510"
     CDS             complement(<1..194)
                     /locus_tag="PK641_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017141367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPG55450.1"
                     /translation="MAKGDLTYTRNIGIMAHIDAGKTTTSERILFYTGLTHKIGEVHD
                     GAATMDWMEQEQERGITITSA"
     gene            complement(217..693)
                     /gene="rpsG"
                     /locus_tag="PK641_03515"
     CDS             complement(217..693)
                     /gene="rpsG"
                     /locus_tag="PK641_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HPG55451.1"
                     /translation="MRKAKPKKRIVLPDPVFGEIMVTRFVNHLMYDGKKSTAYTIFYS
                     ALDSVKAKLANEDKTAIEIWKAALDNITPQVEVKSRRIGGATFQVPTEIRPERKESIS
                     MKNMIHYSRVRGGKTMADKLAAEIVDAYNMQGAAFKRKEDMHRMAEANRAFAHFRF"
     gene            complement(835..1236)
                     /gene="rpsL"
                     /locus_tag="PK641_03520"
     CDS             complement(835..1236)
                     /gene="rpsL"
                     /locus_tag="PK641_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HPG55452.1"
                     /translation="MPTIQQLVRKGRTVLTENSKAPALDSCPQRRGVCVRVYTTTPKK
                     PNSALRKVARVRLTNQKEVNSYIPGEGHNLQEHSIVMIRGGRVKDLPGVRYHIVRGTL
                     DTAGVNGRTQRRSKYGAKRPKAGAAKPTGKK"
     gene            complement(1461..2498)
                     /gene="corA"
                     /locus_tag="PK641_03525"
     CDS             complement(1461..2498)
                     /gene="corA"
                     /locus_tag="PK641_03525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HPG55453.1"
                     /translation="MKRKNSQLVRTYTYTGNSTVDTYIELSLFGQDIPFRTIRCNSSQ
                     EIIAEISANKSLSYWIAIKGLKDISNIEVILKYFKINILHIQDIYNRNHLPKIEEYSE
                     YLFAILNTLTKSETHYEHDNVSLILGNNFVLTFQDTDRGIFDNISQTLSGSQSIITAD
                     HLFSILLNNTTVDFMNDVEMISDMLQNLETHILNFSPNKGMAKELQYARKLLIQLGKS
                     TFPIKEKLGRLSTGENKFIRPENLLYFQDVKDHIIYVVQVISDNKEMLRSLEDLYLAG
                     NDLRMNQIMQRLTVISTIFIPLTFLVGLWGMNFTSMPEIHWQYGYLFAWGIILLVAIL
                     TVWFLKRYKWF"
     gene            complement(2495..2809)
                     /locus_tag="PK641_03530"
     CDS             complement(2495..2809)
                     /locus_tag="PK641_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HPG55454.1"
                     /translation="MKEEQSKNQLNIELSDEMAEGVYANLSIIAHSSSEFVLDFVRIM
                     PNTPKAKVKARIILTAEHAKRLLYALKENVDKYESQYGKIDLPEQKIQIPMNFGGFKG
                     EA"
     gene            2908..4989
                     /locus_tag="PK641_03535"
     CDS             2908..4989
                     /locus_tag="PK641_03535"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPG55455.1"
                     /translation="MKDQVKRKQKIFDEIFKNVLFVLAIVAIICSFPREAKYQYQFEV
                     GKPWQYDLLTATYDAPIYKTKAELKADRDAVLKKNIPYYNYNSAVESEQIKNMETYIM
                     NVSLSEDGINETKDEHAPYATYLKSQLHEIYSKGLLNNNQFTELKTKNTEHIVVLNND
                     GIGRNHATEDIYSVSKALQKIVNNIPSDLSSTKLREFGIENYLKSNLQYNSDITNKIK
                     TDELNNVSTTSGMVQAGERIIDKGEIVTEQKFKILDSLRKDYESRKEYSSEQNKWLIS
                     GQALLVTFLILLFYLYIRLFRKKFYNSKRYIILILILISGFTVLTSLIVKKYSEEYFL
                     FFIPYALLPIILSTFFDTRTALFAHFITILISALMVHSQFEYILLQITAGMVTIYSLK
                     DLTQRSQLIKSALIIFASYCIFYLGYGLTIYKINWEMLLSFSINGIMLLFAYPLIYII
                     EKTFGFISNVTLIELTNTNNSLLRRLSESAPGTFQHSMQVSNLAAEIAIKVDANPLLA
                     RTGALYHDIGKIQNPAFFTENQVKGNNPHDKLSPEGSAQIIMRHVSDGKALAKEYGLP
                     RSVIEFIETHHGKNITKYFYNTWINQHPNETVDESLFTYSGPNPYTKELGILMICDSV
                     EAASRGMTEYTDESINQLVEDIVDGLVYGHFLDNAPLTLKQITLTKSVLKEKLKNIYH
                     TRIAYPEITKK"
     gene            4994..6469
                     /locus_tag="PK641_03540"
     CDS             4994..6469
                     /locus_tag="PK641_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HPG55456.1"
                     /translation="MTRHLLCVLFLTMLTCTANANSNGYISHPSNFNNYTISHTRNTL
                     PERFDSREQSWLLDPRSQQITGPCWAFAVADAVQAYFYKSGFEEGYLSPEILTNCHDG
                     FVFTKSQGGNSYIATACFSRLIGPAYEDAVPYSPLSTECPAHSVDDYPAYVLGAIILP
                     KNDTIAIKQAIYDHGSVTAAIYFANPYYNGDDYSYCYTGTEKPNHGISIVGWDDTERK
                     FIVKNTYGTNSYDEGYFYVSYDDVNIISECFAFEKRVEKSAIDTVYGYDKTGMISKYT
                     YAYKTVSTITRFEAPEKQILNSVGFYIPNPNMKIVIGVLSAEEIIYQSDSLPYTYPGF
                     YTFDLPENTFVENDFYVAIDYPYSIPVENAVENYNDPVIKPIGYQYIQFNNVSAWYDV
                     GVGSQTPSLSNINFCTKAYTRKFTVETPKEAATEDLYVVLEGRINPNIWKDANNIEIY
                     SNAGKLVEIIKKDTEYTYSGFCVLVVHYNDGTTKSVKAILK"
BASE COUNT         2215 a         1145 c         1067 g         2223 t
ORIGIN      
        1 gcagatgtaa tagttatacc acgttcctgc tcttgttcca tccagtccat tgttgcagca
       61 ccatcgtgta cttcaccaat tttgtgggtc aaaccagtat aaaacaatat acgctctgat
      121 gtagttgtct taccggcatc aatgtgtgcc atgataccga tattcctagt gtatgttaaa
      181 tctccttttg ccatttttat ttgtctgtct taattattag aagcggaaat gtgcaaatgc
      241 acggtttgct tcagccatac gatgcatatc ttccttacgt ttaaaggcag ctccttgcat
      301 gttgtatgca tcaactattt cagcagctaa cttatctgcc atagtttttc cacctctaac
      361 tctagagtaa tggatcatat ttttcattga tatggattct ttccgttctg gacggatttc
      421 agtaggtact tggaaggtag caccaccaat tctgcgtgat ttaacttcga cttgcggagt
      481 tatattatct aaagctgctt tccaaatttc gatagcagtt ttatcttcat ttgcgagttt
      541 tgcctttact gagtctaatg ctgaatagaa gattgtgtag gcagtagatt tctttccgtc
      601 atacattaaa tggttaacaa atcttgtaac cattatctct ccgaaaacag gatctggtaa
      661 aacaatcctt tttttaggtt tcgcttttct catttttttt caaaattatc cgttttgttt
      721 ttggttgcca attgctgtct tcaaaagatt cctctgaatc tatttactca accttgataa
      781 gcttttccaa atatgcaaaa caatattatt aactgttttt attaaaattc ctaattactt
      841 tttgcctgtt ggtttggctg cgcctgcttt tggacgtttt gcgccatatt tagaacgtct
      901 ttgagtacgt ccatttacac cggctgtatc taatgtacca cgtactatgt ggtagcgtac
      961 acctggaaga tccttaacac gacctccacg aatcatcacg attgagtgtt cctgcaaatt
     1021 gtgtccttct cccggaatgt aagagttaac ctctttttgg ttagttaagc gaacacgagc
     1081 caccttacgt aaagcagagt ttggcttttt tggagtcgta gtgtaaacac gcacacatac
     1141 tccacgacgt tgtgggcaag aatccaaagc tggagccttg ctattttcgg tcagcactgt
     1201 tctccctttt cttactaatt gttgaatagt aggcattgta acttgttatt atatatgatt
     1261 tacaaaaaat ttatatctaa ttatcaactt gttagctcaa aaactaatta caaattgaac
     1321 caaaagcaca ttttttgggg ttgcaaagtt atgaataaat tttattatac aaaagaaaaa
     1381 taggttttta atgaaatatt ttttcacaaa gccttacaaa tgaagttttg ttaactgaaa
     1441 tatgcagtct ttcaaatgca ctaaaaccat ttatagcgtt tcaaaaacca tactgttaat
     1501 atggcaacaa gcagaattat tccccatgca aataagtaac catattgcca gtggatttca
     1561 ggcattgaag taaaattcat accccataat cctaccaaga atgtaagcgg tataaagata
     1621 gtagaaatta ccgtaagacg ctgcattatt tgattcatac gcaaatcgtt acctgccaga
     1681 tacaaatctt ctaaagagcg taacatttct ttattatcac ttataacttg aactacataa
     1741 attatgtgat cttttacatc ttgaaaatac aacaaatttt cgggtcttat gaatttgttt
     1801 tcgccagtag aaagacgtcc taatttttct ttgattggga atgtactctt acctaactgg
     1861 attaacaact tcctagcata ttgcaattct tttgccattc ctttatttgg agaaaagttt
     1921 agaatgtgtg tctccaaatt ctgtagcata tcgcttatca tttcgacatc attcataaaa
     1981 tctactgttg tattgttaag caatatacta aataaatggt ctgcagtaat tatagattga
     2041 ctgccactaa gtgtttgact gatattatca aaaatccctc tgtcagtgtc ttgaaacgta
     2101 agcacaaaat tatttcccaa aatcagagac acgttatcat gctcataatg tgtctctgat
     2161 tttgttagtg tattaagaat ggcaaacaag tactccgaat actcctcaat ttttggaaga
     2221 tgatttctgt tatatatatc ttgaatgtgt aatatattta ttttaaaata cttaagaatt
     2281 acttctatat tgctaatatc ttttaaacct tttatggcta tccaatatga taaacttttg
     2341 tttgcagaaa tttcagcaat tatttcttgt gaagaattac atcttattgt acgaaatgga
     2401 atgtcttgcc cgaacaaaga caattcaata tacgtatcta cagtactatt gccggtatac
     2461 gtatatgttc ttacaagctg actattctta cgtttcatgc ttcgccttta aatccgccaa
     2521 agttcattgg aatttgaatt ttttgctcag gtaaatctat ttttccgtac tgactctcgt
     2581 atttatctac attttcttta agtgcataca acagtctctt tgcatgttct gcagtgagta
     2641 ttatgcgtgc tttaacttta gcttttggag tattgggcat tattcttaca aaatccaaaa
     2701 caaattcact tgatgaatgt gctatgattg acaaatttgc ataaacacct tcagccattt
     2761 cgtccgaaag ttcaatgttt aattgatttt ttgattgttc ctctttcatg aattactatg
     2821 tttattctgc cacaaattta aaattaatta ttgttatatg cgcataaaaa gttacatttg
     2881 cgaaaaattc actttgattt taactatatg aaagatcaag taaagcgcaa acaaaaaata
     2941 tttgatgaaa tattcaagaa tgtattgttt gtccttgcaa tagtggctat aatatgcagc
     3001 tttccaagag aagccaagta tcaataccaa tttgaggtgg gtaaaccatg gcaatatgat
     3061 ttactaacag caacttatga tgctccaata tataaaacaa aagctgaatt aaaggcagac
     3121 agagatgctg ttctcaaaaa gaatatacct tattataatt ataatagtgc agtagaatcg
     3181 gaacaaataa aaaacatgga gacatacatt atgaatgtct ctttatccga agatggcata
     3241 aatgaaacta aggatgagca cgctccatat gcaacttatt taaaaagcca actacatgag
     3301 atatactcca aaggcctact taacaacaat caattcactg aattaaaaac taaaaataca
     3361 gaacatatag ttgtactaaa caatgatggc ataggaagaa atcatgccac cgaagacatt
     3421 tattcagtat ctaaagcttt acaaaaaata gtaaacaata ttccaagtga tctatcttca
     3481 acaaaattaa gagaattcgg aattgaaaat tatttaaaaa gcaatttgca atacaattcg
     3541 gatataacaa acaaaattaa aactgatgaa ctgaataatg tatcaactac ttcgggtatg
     3601 gtacaagcag gggaacgtat tattgacaaa ggagaaattg taactgaaca aaaattcaag
     3661 atattagatt ctctaagaaa agattatgaa tcacgtaaag agtattcgtc tgaacaaaac
     3721 aaatggttga ttagcggaca agcattatta gtaactttcc ttattttatt gttttactta
     3781 tacataaggt tattccgaaa aaaattttac aatagcaaac ggtatattat tttaatatta
     3841 attttgatat ccggatttac cgttttaact tccttaattg tcaaaaaata ttctgaagag
     3901 tattttctgt ttttcatacc atacgcatta ctacctatta tattaagtac gttttttgat
     3961 actcgtactg cattatttgc acactttatc acaatattaa tttcggctct gatggtgcac
     4021 tcgcaatttg aatatatact attacaaatc actgcgggta tggtaactat ttacagctta
     4081 aaagacctta cacaaagatc gcaactaata aaatctgctc ttattatttt tgcaagctac
     4141 tgcattttct atttaggcta tggattgacc atttacaaaa ttaattggga aatgttatta
     4201 tcattttcaa ttaatggtat aatgttattg tttgcatacc cattaattta cattatagaa
     4261 aaaacttttg gttttatatc aaatgttaca ctcattgagt tgactaacac taataattct
     4321 ttattgagaa gattgtctga atctgcacca ggcacgtttc aacactcgat gcaagtttct
     4381 aatttagcag cagaaattgc aataaaagtt gatgctaatc cgcttttggc acgtacagga
     4441 gctttgtacc atgatattgg taaaattcaa aatccggcat ttttcacaga aaaccaagtt
     4501 aaggggaata atccacatga caaactgagt cctgagggta gtgcacaaat tatcatgcgg
     4561 catgtatctg atggtaaggc actcgcaaaa gaatacggtt taccgcgtag tgtaattgaa
     4621 tttatagaaa ctcatcatgg aaaaaatata actaaatatt tttacaatac ttggattaac
     4681 caacatccta acgaaactgt tgatgaaagc ctttttacat attcaggacc aaatccgtac
     4741 actaaggaat tgggtatttt aatgatttgt gattctgttg aagctgcatc acgtggtatg
     4801 actgaatata ctgacgaaag catcaatcaa ttagtagagg acatagttga cggacttgta
     4861 tatggacact ttttagataa tgcaccatta acattaaaac agataacact gactaaatct
     4921 gtactcaaag aaaaattaaa aaacatatat cacacacgta ttgcttatcc tgaaataact
     4981 aaaaaataat actatgacaa gacatctact ttgcgtttta tttctaacta tgctaacttg
     5041 tacagctaat gctaattcta atggttatat atcgcatcca tcgaatttta ataactatac
     5101 gatttcgcat acaaggaata cacttccgga aagatttgat tcgagagaac aatcttggct
     5161 tcttgatccg agaagtcaac aaataacggg tccatgttgg gcatttgccg tagctgacgc
     5221 tgtacaagca tatttttaca aatccggatt tgaagaaggt tacttatctc ctgagattct
     5281 tacgaactgc cacgacggat ttgtatttac taaaagccag ggtggaaatt cgtacattgc
     5341 aacagcatgt ttctcgcgac taataggacc ggcttatgag gacgctgttc cgtattcgcc
     5401 tttatcaaca gaatgccctg ctcattcagt agatgattat cctgcatatg ttttgggtgc
     5461 aataatttta ccaaaaaacg ataccatagc aattaaacaa gccatttatg atcatggttc
     5521 tgttactgca gcaatatatt ttgccaatcc atattacaat ggcgatgatt attcgtactg
     5581 ctacacagga acagaaaaac ctaaccatgg aataagcata gtaggttggg atgataccga
     5641 gcgaaaattt atagtcaaaa acacctacgg aaccaatagt tacgacgaag gatattttta
     5701 tgtatcttat gatgatgtta acattatatc agaatgcttt gcttttgaaa aacgtgttga
     5761 aaaatcggct atagatacag tttatggcta tgacaaaaca ggtatgataa gcaaatatac
     5821 ttatgcgtac aaaacagtat ctacaattac cagattcgaa gcacccgaaa aacaaatatt
     5881 aaattctgtg ggattctaca ttcctaaccc gaatatgaaa atagtgattg gggtattatc
     5941 tgctgaagaa attatttatc aatctgacag cttaccatac acataccccg gattttatac
     6001 ttttgatttg cctgaaaata cttttgttga aaatgatttc tacgtagcca ttgattaccc
     6061 ttattcaatt cctgtagaaa atgcagttga gaattacaac gatcctgtga ttaagccaat
     6121 aggctaccaa tatattcaat tcaataacgt aagtgcttgg tatgatgttg gtgtaggaag
     6181 tcaaactcct agcttaagta acattaattt ttgcacgaag gcatacacaa gaaaatttac
     6241 agttgaaacg ccaaaagaag ctgctacaga agacttatac gtagttttag aaggacgtat
     6301 aaacccaaac atttggaaag acgcaaataa tattgagata tattccaatg caggaaaact
     6361 tgttgaaata attaaaaaag atactgaata tacctattcc ggattctgtg ttttggtagt
     6421 acattataac gatggcacta ctaaatcggt taaggctata ttaaagtagt atattaacaa
     6481 cattatatta aaaaacactt ataatgctat ttattaatct aaaaagccgt atttttgcag
     6541 ttctaatttg taataagcat gaatttattt gaacacgtag gacgatatgc acaattgatg
     6601 agtggcgtat tttcaagacc tcaacgatgg actttgttgg gaaggcagta
//
LOCUS       DAOPID010000070         6626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_14949_length_6626_cov_5.060721, whole genome shotgun sequence.
ACCESSION   DAOPID010000070 DAOPID010000000
VERSION     DAOPID010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 6626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6626
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14949_length_6626_cov_5.060721"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="PK641_03545"
     CDS             complement(<1..799)
                     /locus_tag="PK641_03545"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HPG55457.1"
                     /translation="MKYLRYCILTIILIHCTALFAQYSSQCPLVNTAFRAGEELEYTA
                     FFQWGGIIVDRLVINTKVTDDIYRKEDVYNISAVGHTEKVMRSFFSLRDTFQVMMRKK
                     DLLSLYFYERDIERKYNAIKNHYFYPIENGMAVEAIEIKNGETKKSTDVFHDICPIDA
                     LGLLYRIRNYDFSNIAVGESTQFTFYRVDGVLNLSITYVGKENISLRDGTTYRCLRFY
                     FETSDGTLFSSENPVSIWLTDDKNKIIVHAEAKLKIGYAKLDLVKVSK"
     gene            complement(802..2643)
                     /locus_tag="PK641_03550"
     CDS             complement(802..2643)
                     /locus_tag="PK641_03550"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPG55458.1"
                     /translation="MKSRTQIIHIILITLLTIITAVLLHSCANRGSGPQGGPKDESSP
                     VIVKSSPNNRARLVSSKQISIEFNELISIEAAYKNVIISPTQKNQPTVKAIGKKAVIT
                     LEDSLLDNTTYTIDFGNSIVDYNEKNVLKDYQFSFSTGADFDTLQISGIVLDAHTLKA
                     MSGISVGIYKTDNDTAFETTPFMRMTKTKEDGSFVIRNIKEGKYKLYALNDVTQTYFY
                     KQIGTDIAFCDSILQPDVHMHGKIDTIWSDSLKTKFDSLIVDAHTEYYPKDLILKSFK
                     ENPIRHFFKKCTRNGKNNFTLTFGATPLKQPVLTIIDSTEHSNWYVQEKAMRADSLTY
                     WITDSLLYKQDTLKLSLIYLKTDSTSNLVEKTDTINMVVTNKIGKKRNKYNEVDNQNL
                     PLSFVHNIVNDMEVYDTLSLNFEEPITEIVKDSIQLFIKKDTIWTQVPFELIKDDSLC
                     TKKLKFLYKKSYGDTYKLNVDSASIRSMYGRTVDKFYKTFRIKQLEEYGNLFIKLPDM
                     PQNGIIELLNQKDQCVQTAFIEDNKYIFQDIKPGPYYIRLIIDENVNGKWDTGIYKDN
                     KQAELVYYYPKQIKIRANWDIEEEWPYNSIPWEKQRPEELKINDSKK"
     gene            complement(2691..4238)
                     /locus_tag="PK641_03555"
     CDS             complement(2691..4238)
                     /locus_tag="PK641_03555"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HPG55459.1"
                     /translation="MASQEDVFKKLVAHCKEYGFVFPSSDIYDGLAAVYDYGQNGVEL
                     KNNIKKYWWDSMILLHENIVGIDAAIFMHPTIWKASGHVDAFNDPLIDNKDSKKRYRA
                     DVLIEDQLAKFDEKINKEVEKAAKKFGDSFDAELYKSTNPRVLENVTKREELHTRFAK
                     ALNDGNLEELKQIILDYDIVCPISGTKNWTDVRQFNLMFTTEMGSTSDGAMKIYLRPE
                     TAQGIFVNYMNVQKTGRMKIPFGIAQIGKAFRNEIVARQFIFRMREFEQMEMQFFVRP
                     GDEIKWFAHWKEFRLNWHKALGLGDEKYRYHDHDKLAHYANAATDIEFEMPFGFKEVE
                     GIHSRTNFDLSQHSKFSGKKIEYFDPELNESYTPYVIETSIGVDRMFLSIMCAAYTEE
                     NLDGGDSRVVLKLPPVLAPIKACVMPLIKKDGLPEKAREIMNELKFDFKCSYDEKDSI
                     GKRYRRQDAIGTPFCITVDHQTLEDGCITVRYRDTMTQDRIKIEELHELIGKAVNMKN
                     LFKKIVG"
     gene            4431..>6626
                     /locus_tag="PK641_03560"
     CDS             4431..>6626
                     /locus_tag="PK641_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HPG55460.1"
                     /translation="MADNDEELKDELETKAHSEYKIPDLKDSMVKHQLSGMFQNWFLD
                     YASYVILDRAVPHLCDGLKPVQRRILHSMKRMDDGRYNKVANIVGHTMQFHPHGDASI
                     GNALVQLGQKDFLIDCQGNWGNILTGDDAAAPRYIEARLSKFALEVVFNSKTTEWKLS
                     YDGRNQEPVALPVKFPLLLAQGTEGIGVGLSTKILPHNFNELIDASIACLKGESFALY
                     PDFVTGGSIDVSRYSDGKRDGKLKVRAKISKLDNKTLCISEIPYGTTTESVIESILRA
                     TEKGKLQIKKVDDNTAANVEILVHLMPGKSSDKTIDALYAFTDCQISVSPNCCVIYEE
                     KPNFLSVSDVLKFSAENTMRLLKEELLIRKTELQESIFMCSLEKIFIEERIYKDKEFE
                     ESKTIESAIAHIDHRLDPFKSSFIRPVEEEDIKKLLEIKMSRILKFNSDKVNEDIKAL
                     YAELEEVKNNLAHIVDFTIKWFDHLKEKYGSKYPRKTEIRNFDNIEVSKVVEANEKLY
                     INREEGFIGTGLKRDEYVCNCSDIDDIAIFYKDGKFKVIKVAEKVYVGKNVLYLGIFK
                     KNDNRTIYNMVYRNGKDGVSYIKRFALGGLARDKDYDLTQGTKGTKVLYFSANPNGEA
                     EILKVFLKPKPRIKNLVFERDFSEIAIKGRSSMGNIFTKNDIHRIVLKQKGGSTLGGR
                     EVWFDYDVLRLNYDGRGTLLGEFCHEDSILVITKRGICFTSNFDLSNHYP"
BASE COUNT         2060 a         1094 c         1167 g         2305 t
ORIGIN      
        1 tcttagatac cttgactaaa tctagcttgg cgtaccctat ttttagtttt gcttcagcgt
       61 gtacaataat tttgtttttg tcgtcagtaa gccaaataga tacaggattt tcactcgaaa
      121 acagtgtccc atcggatgtt tcaaaataga aacgtagaca tctatatgta gtcccgtcac
      181 gcaaactgat attttcttta cctacataag taattgacaa atttaatact ccgtcaacac
      241 gataaaaggt aaattgtgtt gattctccaa cagcgatatt agaaaaatca taatttctaa
      301 ttcggtaaag aagtcctaaa gcatcaatag ggcaaatatc gtggaaaaca tctgtacttt
      361 ttttggtttc tccgttctta atctcaattg cctcgactgc cataccattt tcaatgggat
      421 aaaaatagtg gttcttaatg gcattgtatt tccgttcaat atctcgctca tagaagtata
      481 atgagagtaa atctttttta cgcatcatca cttgaaatgt gtcgcgaagc gaaaagaatg
      541 aacgcatcac cttttcagta tgacctacag ctgagatatt gtaaacatct tctttcctat
      601 atatatcatc tgttactttc gtgtttatta cgagcctatc aactatgatg cctccccatt
      661 gaaaaaatgc tgtatattca agttcttctc ctgctctgaa agccgtattg actaaaggac
      721 attgtgagct gtattgcgca aataaagcag tacaatggat tagtatgatt gttaaaatgc
      781 agtaacgtaa gtacttcatg attatttttt actgtcatta attttcagct cttcaggtct
      841 ttgtttttcc caaggtatac tattgtatgg ccattcttct tctatatccc aatttgctcg
      901 tatctttatt tgtttcgggt agtaatatac aagttctgct tgcttgttat ccttgtaaat
      961 tcctgtatcc cacttaccat taacattttc atcaataatc aggcgtatat agtaaggacc
     1021 cggttttatg tcttgaaata tatatttgtt atcttcaata aatgctgtct gaacacattg
     1081 atctttctga ttaagaagtt ctattatacc attttgaggc atatcgggaa gtttaataaa
     1141 tagattccca tattcttcca attgtttgat tctaaatgtt ttgtaaaatt tatcgacagt
     1201 tcttccatac atacttctta tgctcgctga atcaacattc agtttgtacg tatctccata
     1261 acttttctta taaagaaatt taagcttttt tgtacacaaa ctatcgtctt taatcaattc
     1321 gaatggtact tgagtccata ttgtatcctt ctttataaat aattgtatac tgtcttttac
     1381 gatttctgta ataggctctt caaagtttag cgataatgta tcgtaaactt ccatgtcatt
     1441 aactatattg tgtacaaatg aaagaggaag attttgattg tcgacttcat tatatttatt
     1501 tcgtttttta ccgattttat ttgtaacgac catattgata gtatctgttt tctctaccag
     1561 attactagtt gaatcggtct ttaaatatat caacgaaagc ttcaaggtgt cttgcttata
     1621 caataaagaa tctgttatcc aatatgtaag agaatcggca cgcatggctt tttcttgaac
     1681 ataccaattg ctatgttctg tagaatcaat aattgtgagt acaggttgtt ttagcggtgt
     1741 tgcaccgaag gtcaaagtaa aattattttt accgttgcgt gtacactttt taaagaaatg
     1801 ccttatcgga ttttctttga aagactttaa aatcaaatct ttaggataat attcggtgtg
     1861 agcatcaaca ataaggctat cgaatttggt tttcagacta tcagaccaaa tggtgtctat
     1921 tttgccgtgc atatgaacat cgggttgcaa aatgctgtcg caaaaagcta tatcagttcc
     1981 tatttgctta tagaagtatg tttgtgtaac gtcattcaga gcgtatagct tgtatttacc
     2041 ttcttttatg tttctaatta caaaagaacc atcttctttt gttttagtca tacgcataaa
     2101 tggagttgtt tcaaatgcag tgtcattatc tgttttataa atacctacag atattccgga
     2161 catggctttt aaggtgtgag catctagtac aattccgcta atttgcaagg tgtcaaaatc
     2221 ggcacccgtc gaaaatgaaa attgataatc tttaagtaca ttcttttcat tataatctac
     2281 tatagaatta ccaaagtcaa tggtatatgt agtattatct aataaactat cttccaatgt
     2341 tataacggct tttttaccaa ttgctttgac ggtaggttga tttttttgtg tgggagaaat
     2401 tatcacattt ttgtatgccg cctcaattga aatcaactca ttaaattcta ttgaaatttg
     2461 ctttgaagaa accagtcttg ctctattatt gggtgaactc ttaactataa caggagatga
     2521 ttcgtctttt ggacctcctt gtggtccgga acctctgttt gcacaagaat gtaacaatac
     2581 ggctgtaatt attgtcagca gtgttattaa aatgatgtga attatttgag ttcttgattt
     2641 catagaggca aaataaggga cactctaaaa aaaatgtccc cgtaaattat ttatccaaca
     2701 atttttttga atagattttt catattaacc gctttaccta taagttcgtg caattcttca
     2761 atcttaatgc ggtcttgtgt catagtgtct ctgtaacgaa ccgtaataca tccatcttct
     2821 aatgtttgat gatctacagt gatacagaat ggagtaccaa ttgcatcttg acgacgataa
     2881 cgtttcccta tagaatcttt ttcgtcataa gaacatttga aatcaaattt cagttcgttc
     2941 ataatttcac gagctttttc aggtagtccg tctttcttaa tcaatggcat cacgcaagcc
     3001 tttattggag ccaatacagg tggtaatttc agtactacgc gactatctcc tccatctaag
     3061 ttctcttcgg tatatgctgc acacattatt gataagaaca tacggtctac acctattgaa
     3121 gtttctatta cataaggagt atatgattca tttaattcag gatcaaaata ttcaattttt
     3181 ttacctgaga attttgaatg ttggcttaag tcgaagtttg tacgagagtg aataccttct
     3241 acttctttaa aaccaaaagg catttcaaat tctatgtcgg tagctgcatt tgcgtagtga
     3301 gctaatttgt cgtggtcgtg gtaacgatat ttctcatcgc ccaagcccaa tgctttgtgc
     3361 caatttaatc taaattcttt ccaatgggca aaccatttta tttcatctcc cgggcgaaca
     3421 aagaattgca tttccatttg ctcgaactca cgcattctaa aaataaattg tcttgctacg
     3481 atttcgttac ggaaagcctt tccgatttgt gctattccga aaggaatttt catgcgaccg
     3541 gtcttttgta cattcatgta atttacaaaa ataccttgtg cagtttccgg acgtaaatag
     3601 attttcattg caccatcaga tgtagaaccc atttctgtgg taaacatcaa attaaattga
     3661 cgcacatccg tccaattttt cgttccgctg atagggcata caatgtcgta atcaagtatt
     3721 atttgtttta attcttctaa attaccatcg ttaagagcct tagcgaagcg tgtgtgtaat
     3781 tcttctcgtt tagttacatt ttcaagaaca cgcggatttg tgcttttgta taattcagca
     3841 tcaaaagaat cgccaaattt cttagctgct ttttcaactt ctttatttat tttttcgtcg
     3901 aattttgcta attgatcttc aataagaacg tcagcgcgat agcgtttttt gctgtctttg
     3961 ttatctatta gaggatcgtt aaaagcgtca acgtgacctg atgctttcca aatagtagga
     4021 tgcataaaaa tagctgcatc aataccaaca atgttttcgt gtagcaatat catgctgtcc
     4081 caccaatact ttttgatatt gtttttaagc tcaactccgt tttgtccgta atcatataca
     4141 gcggcaagtc cgtcgtaaat atcactggaa gggaatacaa aaccatactc cttgcagtga
     4201 gcgactaatt ttttaaatac atcttcttgt gatgccataa ttatattgcg attaattgtt
     4261 tattgtgcta attttatatg cttttttgta ctaaaaaata tgtttctgct tttttgtttt
     4321 cagagccatt atttttgaaa tgcaaaggta aggtttttga tggatttttt gtaattttac
     4381 gccgaaactt gcacggatag catgcaagat gaacaaaaaa gattcaaaag atggcagata
     4441 atgatgaaga attgaaagac gagctggaaa ccaaagccca ttctgagtac aaaatacctg
     4501 atttaaaaga ctcgatggta aaacatcaat tgtccggtat gtttcaaaat tggtttctgg
     4561 attatgcatc ttacgtaatt ttggataggg cagttccgca cttatgtgat ggtctaaagc
     4621 ctgttcaaag gcgtatttta cattctatga aacgcatgga tgacggacgc tacaacaaag
     4681 tagctaacat agttggtcac actatgcaat ttcatcctca tggagatgct tcaattggaa
     4741 acgcattagt tcaattaggt caaaaagatt ttttgataga ttgtcaagga aactggggaa
     4801 atattttgac cggtgatgat gcagctgccc ctcgttatat tgaagctcgt ttgagcaaat
     4861 ttgctttaga agttgtattt aattctaaaa caacagagtg gaaattgtct tatgacggac
     4921 gaaatcaaga gcctgtagct ttacctgtta aattcccatt attgttggca caaggaactg
     4981 agggaatcgg cgtcggactt tctacaaaga tattacctca taatttcaat gaacttattg
     5041 atgcatctat tgcttgcctt aaaggagaat catttgcatt atatccggat ttcgtaacag
     5101 gaggttcgat tgacgtatct cgttatagcg atggaaagcg tgatggaaaa ttgaaggtac
     5161 gtgctaaaat ctcaaaactc gataataaaa cattatgcat ctctgaaatt ccttacggaa
     5221 caacaacaga aagtgttata gaatcaattc tgcgtgctac tgaaaaaggt aaattacaaa
     5281 ttaaaaaagt agatgataac acagctgcaa atgtcgagat attagttcat ttaatgccgg
     5341 gtaaatcttc ggataagact atagatgcat tatatgcttt tacagattgt caaatcagcg
     5401 tatctcctaa ttgttgtgtt atttatgaag aaaaaccaaa tttcctttct gttagtgatg
     5461 tgttaaagtt ttcggctgaa aacacaatga gattgttaaa ggaagaattg cttatacgca
     5521 aaacagaatt acaagaatca atcttcatgt gttcgctcga aaaaatattc attgaagaac
     5581 gtatttataa ggataaagaa tttgaagaaa gtaaaactat tgaatctgct attgctcaca
     5641 tagatcatcg tttagatcca ttcaaatcta gttttataag accggtcgag gaagaagata
     5701 ttaagaaact attggaaatc aagatgagtc gtatcttgaa atttaattct gacaaggtta
     5761 atgaagatat caaagcccta tatgctgaat tagaagaggt taagaataac ttagcacata
     5821 tagtagattt tacaataaaa tggttcgacc atttaaaaga aaaatatggt agcaagtatc
     5881 ctcgtaaaac cgaaataaga aattttgata atattgaagt ttcgaaggtt gtagaagcaa
     5941 acgaaaaact ttatatcaat agggaagaag gctttatagg aaccggactt aagcgcgacg
     6001 aatatgtttg taattgttct gacattgatg acattgcaat attttacaaa gacggtaagt
     6061 ttaaggttat taaagttgca gaaaaagtat acgttggaaa gaatgtattg tatttaggta
     6121 tctttaaaaa gaatgataac agaaccatat acaatatggt ttacagaaat ggcaaagatg
     6181 gtgttagtta tataaaacga tttgctttag gtggtttggc tcgcgacaaa gattacgatt
     6241 tgactcaggg aacaaaaggt acaaaagttc tttatttttc agcaaatccg aatggtgagg
     6301 ctgagatttt gaaagtattt ttaaaaccta agccgcgtat aaagaatctt gtatttgagc
     6361 gtgattttag cgaaatagct atcaaagggc gttcttctat gggtaatatt tttactaaaa
     6421 atgacattca cagaatagta ttgaagcaaa agggaggttc aactttaggc ggaagagaag
     6481 tgtggttcga ttatgatgtt ttgcgtctta attatgatgg gcgcggtacc ttacttggag
     6541 agttctgtca tgaagattcg atacttgtca taactaaacg tggaatttgt tttacaagta
     6601 actttgattt aagcaaccat tatcct
//
LOCUS       DAOPID010000071         6568 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_15143_length_6568_cov_4.059266, whole genome shotgun sequence.
ACCESSION   DAOPID010000071 DAOPID010000000
VERSION     DAOPID010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 6568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6568
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15143_length_6568_cov_4.059266"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PK641_03565"
     CDS             complement(<1..237)
                     /locus_tag="PK641_03565"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55461.1"
                     /translation="MQRKVIKKLYGVIGNPLRHSYSPAFFQEKFKEKHLDSSSYVKFE
                     LNRIEDITNVLDKNSNLRGLNVTIPYKEAIIPYLT"
     gene            complement(222..944)
                     /locus_tag="PK641_03570"
     CDS             complement(222..944)
                     /locus_tag="PK641_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HPG55462.1"
                     /translation="MKKLLIILFFCCSLSSLNAQIDKTNLVKDNPSLIQKIEAWYEDN
                     TNYYTITALMAVESSFIPFPSEIVIPPAAYIASKPDSDLNIFLVILFGTIGAIIGAIF
                     NYYLALWLGRPIIYKFADSKIGHLCLLSKEKIQKAEEYFVANGNISTLVGRLLPGIRQ
                     LISIPAGLAKMNIWNFLLYTTIGSGAWNCILALLGYLAHGQQDLINQYSHELSIVLIC
                     FGVLFIIYLIIKGISKKKCNAK"
     gene            complement(941..2335)
                     /locus_tag="PK641_03575"
     CDS             complement(941..2335)
                     /locus_tag="PK641_03575"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG55463.1"
                     /translation="MNFSNSKSEIVKRYEDRNDGYFDVNEISEVVDYYLRRLRTKDAK
                     KAIEIGLQLHPDDWELHYQKGRILLDLQEYDSAYAVTNEMFELCKSNNEITDIDAMAD
                     TILLKGETLVRMGNDKQAKFVFQELLQDKYGKLDDAYLDIADTYITLNDHETALQYLQ
                     EGLKYNQKNTSIISEISFCYKQLRQLDKAIEYCNKCLDISPYSSDSWYNLGELYFSKE
                     LYPEAIDAFDFTTAIDDTDVSAWEMKAYALYYNENYRKAAECHLKCAQLNGSNDASSL
                     AFAAEAFEKAEDYDNGIRYYREVLKLQPENTDAICGIGLCLFELKKYEECIVHFKKAI
                     ELDSYLSDNWMYLGEAYAALDNVEESIFAYNQCLMRKDNQAAVWATLGNLHFETDNFE
                     LALHCYKKAIEYDPNLLYIDLFLSLTYYGLGKMVLAAEHLKTAIINNANSTDLFLEVY
                     PDAHAFILTINTNS"
     gene            complement(2388..3494)
                     /locus_tag="PK641_03580"
     CDS             complement(2388..3494)
                     /locus_tag="PK641_03580"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPG55464.1"
                     /translation="MFSKPFTLDRTVRIVGVIIIIIGTVLLINKLSGVLLPFFAAWLI
                     AYMINPMVEFVQNKMHFKNRLISVLTIMLLFLGIIVGIGFLAANSIEKELIEVDKLVD
                     TYLAQSGKTEFAPFIQDYISQFLQNIDFDMLLTREKIVETIEKVIPKAFDIMSSSLHY
                     ITGLVVIFIMILYMFFILVDFKSLSTDWEVLIPKRYRKFSRQLFSDLASGMNIYFRKQ
                     ALISSIVGCLFAIGFSIIGLPMGVAMGLFVGLLNMIPYLHTLGIIPPLLIALIQSAQS
                     GSGFWMTALWIVLTFCIIQVILDGFLTPKIMGKATGLNPAVILLSLSIWGSLMGVLGM
                     IIALPVTTLMISYYKHFVIGENNDKLEDVNNLKV"
     gene            3560..4033
                     /locus_tag="PK641_03585"
     CDS             3560..4033
                     /locus_tag="PK641_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55465.1"
                     /translation="MRQMNKQVLLDDIKKRMILIQEQIKQLNYQIEQLSACLDEDDSS
                     KKNNKVEFKSVPNQFTPVSEKIDNNIAQPQLKLVDARLIVDLQKAIGINDRFRFKNDL
                     FKGDAQLMSDTFDVLNNVDTFEDAMSYIRSHFDWSETNDTVIYFFDLLHRRYYRS"
     gene            4030..4701
                     /gene="rsmI"
                     /locus_tag="PK641_03590"
     CDS             4030..4701
                     /gene="rsmI"
                     /locus_tag="PK641_03590"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HPG55466.1"
                     /translation="MKLYIVPTPVGNLDDMTFRAIKVLKEVDFILAEDTRTSSVLLKH
                     FEISNKLIPHHKYNEHQSLMTVIDRLLAGQQGAVISDAGTPGLSDPGFLIVRECVSKG
                     IEVECLPGATAAIPALVNSGLPMDRFCFEGFLPQKKGRQTRLNSLKEESRTMVFYESP
                     YRVRKTLEQFAAIFGADRLASVSREISKIHNETKYGTLEELMSYFTENEPRGEFVIVV
                     CGLKK"
     gene            4736..5587
                     /locus_tag="PK641_03595"
     CDS             4736..5587
                     /locus_tag="PK641_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55467.1"
                     /translation="MKKIFLGCAVLCVLFTSCDYESKAYKALKMQNDSIMAVQERQSA
                     ELDEYLSIIQDVDSGFEAIRQSQNYMTVTSSTEGDPTSEMRNRVADNMYMINSILADN
                     KAKIEELEKKLDNGNIKSTQLKKTVERLSATLEQKNAEITALQKELEQKDYKIDSLLL
                     ENQMINSQANDLAAENYAQQKTLQEQDAQIHRVYYLIANRKTIKSNNIDVKNMRSSLR
                     TSLFTTADMRQLDRLDTNSKYAKILTKHPTTSYELIKGSDRKYSLIIKNPSDFWSTSK
                     YLLIQID"
     gene            5637..6338
                     /locus_tag="PK641_03600"
     CDS             5637..6338
                     /locus_tag="PK641_03600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HPG55468.1"
                     /translation="MRSPYKHLFFDWDNTLWNFSANAEESLKVLFFQNGLNAYFDSFD
                     EYYLLYKKRNDELWPLYSLGKINKDYLNTERFVYPMQEKGCLNKTKSDAMQRDYLPLL
                     AKQTRLIDGASEVLEYLKNEGYKLYVISNGFSEVQYIKIEVSGLQKYFTKIYLSENIR
                     EHKPSRLFFDYVIKSSNARKSESLVVGDNWDADIVGAKNAGIDQVLFSPAGNASITFT
                     PNYIIHDLRELMSFL"
     gene            6335..>6568
                     /locus_tag="PK641_03605"
     CDS             6335..>6568
                     /locus_tag="PK641_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007218976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPG55469.1"
                     /translation="MRLIDTHSHIYSEEFSADRDEVISRALTAGVEHIFMPNVDSTSI
                     AAMLETRNKWPELCHVMMGVHPTSVNANYRDELA"
BASE COUNT         2218 a         1025 c         1124 g         2201 t
ORIGIN      
        1 tgtaaggtaa ggaataatag cttctttata tggaatagtc acatttaatc cacgaagatt
       61 ggagttttta tccaatacat tagtaatatc ttctatcctg tttagttcaa attttacata
      121 agaagaagaa tcaagatgtt tctctttaaa cttctcttga aaaaaagcag gtgagtaaga
      181 atgtctcaga gggttgccta ttactccgta taatttctta attactttgc gttgcatttc
      241 ttttttgata ttcctttaat tataagataa ataataaata gtacaccaaa acatattaat
      301 actattgata attcgtggct atattgatta atcaggtctt gttggccatg tgcaagatat
      361 cctagtaatg ccaatatgca attccatgct ccggatccta ttgttgtgta taatagaaaa
      421 ttccatatat tcatctttgc cagacctgcc ggtattgaaa taagttgtct tataccggga
      481 agtaaacgtc ctaccaaagt tgagatattt ccgtttgcaa caaaatattc ttctgccttt
      541 tgtatttttt ctttactgag tagacatagg tggcctatct tactgtctgc aaacttatat
      601 ataataggtc tacctagcca taaagctagg taataattaa aaatagctcc aataatagct
      661 cctattgtac caaaaagtat aactaagaaa atatttagat ctgaatcagg tttgcttgca
      721 atatatgcag ccggtggaat tacaatttct gacgggaaag gaataaaaga actctcaacg
      781 gccatcaatg ctgttattgt ataataattt gtattgtctt cataccatgc ttcaattttt
      841 tgaattaaag atgggttatc cttaactaag tttgttttgt caatctgtgc atttaatgag
      901 gataatgagc aacaaaaaaa tagaataatc aaaagttttt tcatgagttg gtatttattg
      961 ttaatataaa cgcatgtgca tcaggatata cttctaaaaa taagtctgtg ctatttgcat
     1021 tgtttatgat tgctgttttt agatgctcag cagctagtac catcttacct aatccgtaat
     1081 atgttaaaga caaaaaaaga tcgatgtata gtagattagg atcgtactct atggctttct
     1141 tatagcagtg taatgccagt tcgaaattgt ctgtttcaaa atgtaaattg cctaatgtag
     1201 cccacactgc agcttgattg tctttacgca ttaagcattg attatatgca aatatgcttt
     1261 cttctacatt atccaatgct gcatatgctt ctccaagata catccaatta tcgcttaaat
     1321 agctatcaag ttctatcgct tttttaaaat gtacgataca ttcttcatat ttttttagct
     1381 caaacaaaca taatcctatt ccacaaattg catctgtgtt ttccggttgt agtttaagca
     1441 cttcacgata atatcttata ccattatcat aatcttcggc tttttcaaat gcttcggcag
     1501 caaatgccag cgaggatgca tcatttgaac catttaactg agcgcatttg aggtgacatt
     1561 ctgcagcctt tctataattt tcattataat ataatgcgta ggctttcatt tcccatgcac
     1621 taacatctgt gtcatcaatg gctgtagtaa aatcaaatgc atcaattgct tctgggtata
     1681 gttctttaga aaaatataat tctccaagat tataccaaga atcagatgaa tatggtgata
     1741 tgtctaagca tttgttacaa tattcgattg ccttatctaa ctgtcttaat tgtttgtaac
     1801 aaaatgatat ttcagatatg attgatgtgt ttttctggtt gtattttaga ccttcctgca
     1861 aatattgaag cgcagtttcg tgatcgttta atgtaatata tgtgtcagca atgtctaggt
     1921 atgcatcgtc taatttgccg tacttatctt gtaataattc ttgaaaaaca aattttgctt
     1981 gcttgtcatt tcccattctt actagggttt ctccttttaa caaaatagta tcggccattg
     2041 catcaatatc ggttatttcg ttgttagatt tgcatagttc aaacatttcg ttagttactg
     2101 cgtaagcgct atcatattct tgcaaatcaa gcaaaatacg acctttttgg taatgcaatt
     2161 cccaatcgtc agggtgtagt tgtaaaccta tttctatagc ttttttggcg tcttttgtac
     2221 gaagcctacg taaataataa tcaactactt cactgatttc attgacatca aaatagccat
     2281 cgttacgatc ttcataacgt tttactattt cagatttgct atttgaaaaa ttcattattc
     2341 gtgtattctg ttgcaaaagt aagaataaag gaacgaattt tatgatttta gaccttcaag
     2401 ttattaacat cttccaactt atcgttattt tcgccgataa cgaaatgctt ataatatgaa
     2461 atcatcaatg tcgttacggg tagagcaata atcattccaa gtactcccat tagagaaccc
     2521 caaatagaaa gagataataa tattactgca ggatttaatc cggttgcttt acccattatt
     2581 tttggagtta agaaaccatc caatatgact tgaatgatgc aaaatgtgag aacaatccac
     2641 aatgctgtca tccaaaatcc cgaacctgat tgtgctgatt gtataagagc gatcaataat
     2701 ggagggataa tacccaaagt gtgcagatat ggaatcatat tgagtaaacc aacaaaaaga
     2761 cccatggcta cacccatagg aagcccgata atactaaaac cgattgcgaa tagacatcct
     2821 acgatactcg atattagtgc ttgttttctg aagtaaatat tcatgcctga tgccaaatcg
     2881 gaaaatagtt gtctggaaaa ttttctatag cgttttggta tcaaaacttc ccaatcggta
     2941 gaaagtgatt taaagtctac gagtataaaa aacatatata gaatcattat aaatataacc
     3001 actaaaccgg taatgtagtg caatgaactt gacattatgt caaatgcttt aggtataact
     3061 ttttctatgg tttctacaat tttctcgcgt gtaagcaaca tatcaaagtc tatattttga
     3121 agaaattgtg atatgtaatc ttgtataaaa ggagcaaatt ctgttttgcc tgattgtgca
     3181 agataggtat ctactaattt atcgacttct attaattctt tttcaatcga attggctgct
     3241 aaaaaaccta tccccacaat tatacccaaa aacaacaaca tgatagtaag tacacttatt
     3301 aatctattct taaaatgcat tttattttgc acaaactcaa ccataggatt tatcatatat
     3361 gctattagcc atgctgcgaa gaaaggaagc agaacaccgc ttagcttatt gataagtaat
     3421 acggtgccta ttattataat aatcacgcct actatgcgta ctgtgcgatc taatgtaaat
     3481 ggtttactga acataatgtg agtgtgtgat tcgttttatt tataaaatta ctatttttgc
     3541 aaaagtaaat aaaaaaatta tgcgacagat gaataaacaa gttttgttag atgatattaa
     3601 gaaaagaatg atcttaattc aagaacaaat taagcaactg aattatcaaa tagaacaatt
     3661 gagtgcttgt ttggatgaag atgattcttc gaaaaaaaat aataaagttg aatttaaatc
     3721 tgttccgaat caatttactc ctgtctctga aaaaatagat aataatattg ctcaaccaca
     3781 gcttaagctt gtggatgcca gattaattgt tgatttacaa aaggcaatag gaatcaatga
     3841 tcgttttcgt tttaaaaatg acttgtttaa aggcgacgca caacttatgt cagatacgtt
     3901 tgacgttctt aataatgtag atacttttga agatgctatg agttatattc gtagccattt
     3961 tgattggagt gaaactaatg ataccgtaat ttactttttt gatttgttac atcgtagata
     4021 ttatcgttca tgaaattata tatagtgcca actcctgttg gtaatttaga cgatatgact
     4081 tttagagcta taaaagtttt aaaagaagta gattttattt tagctgagga tactagaact
     4141 agtagtgtgc tgctaaaaca ttttgaaata tcaaacaagc ttattccaca tcataaatac
     4201 aatgaacacc agtcattaat gactgtaatc gacagattgt tggcaggtca gcagggagct
     4261 gttatttctg atgcagggac tccgggtttg tcagaccccg gatttttaat agtacgcgaa
     4321 tgcgtttcta aaggtataga agtagagtgt cttcccggtg ctacggctgc aatacccgcg
     4381 cttgtaaatt caggcttacc aatggataga ttttgttttg aaggattctt acctcaaaaa
     4441 aaaggtcgtc aaacaagatt gaattctttg aaagaagaaa gtcgaactat ggtgttctat
     4501 gaatcacctt atagggtacg taaaactcta gaacaatttg cagccatatt cggagccgat
     4561 agactagcat cagtatcacg cgaaatatca aaaattcata acgaaaccaa gtatggtaca
     4621 ttagaagaat taatgagtta tttcacagag aatgaaccac gtggagaatt tgttattgtt
     4681 gtatgcggat tgaaaaaata gttatatctt tgcggcatta aaaatcaaca aaaacatgaa
     4741 aaagattttt ttaggatgtg cagttctatg tgtcctgttt acatcatgtg attatgaatc
     4801 aaaagcttat aaagctttga aaatgcagaa tgattctatt atggcggtac aagagcgcca
     4861 atctgcggaa cttgatgagt atttgtctat tattcaggat gttgattccg gttttgaggc
     4921 aattcgtcaa tctcaaaatt atatgactgt tacctcaagt acagaaggtg atccaacttc
     4981 cgaaatgaga aatagagttg ctgataatat gtatatgatt aatagtattt tggctgataa
     5041 taaagctaaa attgaagagt tggagaaaaa acttgataat ggtaatatca aatctactca
     5101 acttaagaaa acagtagaac gattgtcagc tactttggaa caaaaaaatg cagaaattac
     5161 agcattgcaa aaagaattag aacaaaaaga ttataaaatt gacagcttgt tgctcgaaaa
     5221 ccaaatgatt aattctcaag ctaatgattt agctgcagaa aattatgcac agcaaaaaac
     5281 attgcaagag caagatgctc aaatacatcg tgtttactac ttaattgcca acagaaaaac
     5341 aattaaatca aacaacattg atgttaagaa tatgcgctca agcttgagga caagtttgtt
     5401 tacaactgct gatatgcgtc aattggacag attggataca aattcaaaat atgctaagat
     5461 tttgacaaaa catccgacta catcttacga gttgattaaa ggatcagata gaaaatactc
     5521 tttgataatc aaaaatccta gtgatttttg gagtacttca aaatatttat tgattcaaat
     5581 agactaatat aataaaacaa aactatgtta atggatgatt cattaatgca tattatatga
     5641 gatctcctta caagcatttg ttttttgatt gggataacac cttgtggaat ttttctgcta
     5701 atgcagaaga gtccctcaag gtgttgtttt ttcaaaatgg attgaatgct tattttgact
     5761 cttttgatga gtattacctt ttgtacaaaa aacgaaatga tgaactatgg cctttgtatt
     5821 ctttaggtaa gatcaataaa gattatttaa atacagaacg atttgtttat cccatgcagg
     5881 aaaagggatg tttaaataaa actaagtctg acgcaatgca gcgcgattat ttgcctctgc
     5941 tagcaaaaca aacgcgttta atagatggag catccgaagt tctagagtat cttaaaaatg
     6001 aaggctataa attgtatgtt atctcgaatg gctttagcga agtacagtat attaaaatag
     6061 aagtatccgg tttacagaag tactttacaa aaatatattt gtcagagaat attagagagc
     6121 ataaaccaag tcgcttgttt tttgactatg tgattaagtc gtcaaatgcc agaaaatcag
     6181 agagtcttgt tgtcggtgat aattgggatg ccgatatagt aggggcaaaa aatgcaggta
     6241 ttgaccaagt gttattttct ccagcgggaa atgcttcgat tacatttact ccgaattata
     6301 tcattcatga tttacgtgaa ttaatgtctt ttttatgaga ttaatagata cgcattcaca
     6361 catttattca gaagaatttt cagctgatag ggatgaagtt atttcaagag cactaacagc
     6421 cggtgtagag catattttta tgcctaatgt agattctaca agtattgctg cgatgttgga
     6481 aactagaaat aaatggcctg aattatgtca tgtaatgatg ggagtgcacc ctacatcagt
     6541 taatgcaaat tatagagatg aattagct
//
LOCUS       DAOPID010000072         6495 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_15405_length_6495_cov_4.674689, whole genome shotgun sequence.
ACCESSION   DAOPID010000072 DAOPID010000000
VERSION     DAOPID010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 6495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6495
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15405_length_6495_cov_4.674689"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1452)
                     /locus_tag="PK641_03610"
     CDS             complement(<1..1452)
                     /locus_tag="PK641_03610"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55470.1"
                     /translation="MKRNYTNILQLIALLFVSILCCTQNVSAAYTLAVYPNANPTDIQ
                     HFDFVQKPSNVPDEMRVVANFSGNANDYSFWVGQDYAKDAYSADVPLSNVILNCTTGS
                     FTTSIYTTNTAANHFPHDFICLDTDEYIYGMACWYDIAANCGGGASSAGNHVFTTNDH
                     VVWTYSFPLVNVSSGFKILKSADGVNWAWNMGQLGHEQSRTFLTEGTGVNINITASGW
                     AGDCTLDLPSIANGDTVLLSLTKSGANYAISASYELAKPEISTDDSDCSNVVFEITNY
                     QASRTYTCTLDGSPVTLTNGVYSINNPQPNTTYIFEAVVSEGALTSEVSTASFLYEGN
                     PDPPVVQSYRECQQVGTATWSSLVNCPSCNLLWYDSQISINPISEPSSFDKNTVGVTT
                     YYVSQVVDGCESSRQEVSVTVYELPNPLVGLPQSICSGSVAQLGSSPVADVDYSWSPA
                     DKLDDATIANPTTLPLTSSQEFTLTVQKHSYPVP"
     gene            complement(1562..2509)
                     /locus_tag="PK641_03615"
     CDS             complement(1562..2509)
                     /locus_tag="PK641_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPG55471.1"
                     /translation="MEEIIQAVDRKLLKSELTPDKFLRKTNKGGNEIYITTAKDSPNI
                     MLELARLRELSFRSAGGGSGKSLDMDEFDTMECPYKQLFVWDPDSEEIIGGYRYIMAN
                     DIKLSENGQPHLATAEMYYFTDNFIKNYLPYMIELGRSFVQPDYQSSKKGAKSLFALD
                     NIWDGLGALLVKNPNLKYFFGKVTVYQHYNSTARDMIFGFLRKYFPDHENLVYPVDPF
                     EISFTPKQIDNLFNAPTYKENFHILNREVRALGVNIPPLVNAYMGLSPNMSSFGFAVY
                     HEFGELIECCIMIPIDQMYEEKKQRHITTFLEGPENKTV"
     gene            complement(2518..3324)
                     /locus_tag="PK641_03620"
     CDS             complement(2518..3324)
                     /locus_tag="PK641_03620"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol acyltransferase"
                     /protein_id="HPG55472.1"
                     /translation="MNGIALIDVASILSSRTGKKPSRLVVAILKRLIHQDWINDVLLK
                     IGTRKDVDFMCAVVEAMDIHLEVKGLENLPEPGNRTMFVCNHPLGGVDVVSVVSVVGV
                     KYPEGLLIPANDFLLHLKNIKDMLIPVNKVGGQSKELSDRLNAAYASDAQLMFFPAGR
                     VSRKHKGIIADDEWKKNFVSKSVEYHRQVLPIRIDAQNSRLFYFVARIRKIIGLKLNI
                     EMLMLPRELYRQRGKTIKITIGKAVAWKNLDSTKSPKVLAQEIRAMVYNL"
     gene            3562..4023
                     /locus_tag="PK641_03625"
     CDS             3562..4023
                     /locus_tag="PK641_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HPG55473.1"
                     /translation="MPSFIGNIEAKVDAKGRAFVPAQFRRLVDENSVFVLRKGIFDDC
                     LVLYPEAVWEEIIGVMRQKLSRWNRKEEQVFRQFVAEANRIELEDNGRMLIPKRYIES
                     IGIKNDIRFVGCDTSIEIWPSNKIEETFISADSFAAELEKLMEKPDNSNNN"
     gene            4096..4995
                     /gene="rsmH"
                     /locus_tag="PK641_03630"
     CDS             4096..4995
                     /gene="rsmH"
                     /locus_tag="PK641_03630"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016271911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPG55474.1"
                     /translation="MSELYHTPVLLNASIDGLNIGKGGVFIDTTFGGGGHSREILNRM
                     DASGKLLAFDQDKDTIANIPNDSRFIFAQSNFRYISNFVRYYNLYPVNGILADLGVSS
                     HDFDEAERGFSFRFNGKLDMRMNQRAFLTAETIINHYDAEHLANVFYLYGELNQSRKI
                     ARAIIKKREKKAFNEIFELVDCVKPFCGKDEKKDLARIFQALRIEVNDEMGALKTMLL
                     SSEKVLVKGGRLSIITYHSLEDRLVKNFMKTGNFDGKQEKDFYGNLISPFCTIGKITI
                     PDTEEIIRNPRSRSAKLRIAEKK"
     gene            4992..5372
                     /locus_tag="PK641_03635"
     CDS             4992..5372
                     /locus_tag="PK641_03635"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HPG55475.1"
                     /translation="MTDNSKKIISKILNKDEVKDLKETSIKDVISGNIFTKSYFLKQT
                     KLILLIIALVFIYMNNRMVCEKQLALIDKLNKELECEKYISMVTETELLSISRPEEIK
                     KLVDKHGLQLEQPTMPPYKVIIKE"
     gene            5372..>6495
                     /locus_tag="PK641_03640"
     CDS             5372..>6495
                     /locus_tag="PK641_03640"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPG55476.1"
                     /translation="METAIYIALILLIVGLIVLAIIVLTRKKDVKTDILVRFGFVFVV
                     ILALFGIVFGRIIYLQNVEGDALRKLADKRISKPDTIKAKRGNILSDDGRLMASSLPT
                     YYLYMDMRVEALRIIKKGDTKNYFQLHVKELADALAKKFGDKTAKEYERDLTRAYKNK
                     SAQYKIYPKRVSYLDYMEIKKFPILHRGSIQGGFFTEERVTRVKPFGSLASRTIGDIY
                     GIEEKGGKNGLELQYDSVLRGTEGLKVTRKMAGKRTDIILKDPINGIDVVSTINIDLQ
                     EAAEKSLRNELTECQASCGTVVLMDVKTGAVKAISNLTRNPNGTYSEVTNIAVASQIE
                     PGSTFKTLSLLVALEDGVIDTTTMINTENGGHQFANRIMK"
BASE COUNT         2252 a         1116 c         1143 g         1984 t
ORIGIN      
        1 aggtacagga tatgaatgct tttgtacagt tagtgtaaat tcctgacttg atgtcaaagg
       61 cagtgttgta gggtttgcaa ttgttgcatc atctaatttg tcggcaggtg accatgaata
      121 atcaacatcc gcgacagggc ttgaacccaa ctgagctaca cttcctgaac atattgattg
      181 cggcaaacct accaaaggat ttggcaactc gtacacagta acagaaactt cttgacgtga
      241 actttcacaa ccatcaacta cctgtgatac gtaataagtt gtaactccta ctgtattttt
      301 atcgaatgat gatggttctg aaatagggtt aatagatatc tgtgaatcat accaaagcaa
      361 attgcatgat ggacaattaa ctaaagatga ccatgttgct gttcctactt gctgacactc
      421 tcgatatgat tgaactaccg gaggatcggg attgccttca tacaaaaaag aagctgtgga
      481 cacttcagat gtaagagcac cctcagaaac tacagcctca aaaatgtaag tagtattagg
      541 ttgcggatta tttattgaat aaactccatt tgtaagtgta acgggcgaac catctaaagt
      601 acaggtgtat gttcgtgaag cttgataatt agttatttca aaaacaacgt ttgaacaatc
      661 gctatcatct gtcgaaattt caggtttagc taattcataa ctagctgata ttgcataatt
      721 ggcaccactt tttgtcaagc tcaacaatac tgtatcgccg ttagcaatac taggcaaatc
      781 aagcgtacaa tcacctgccc atccggaagc tgttatatta atattaacac ctgttccttc
      841 agttaagaat gttcttgatt gttcgtgtcc taattgtccc atattccaag cccaattaac
      901 tccatcggca gattttaata tcttaaagcc tgatgaaaca tttacaagcg ggaaagaata
      961 cgtccataca acatgatcat tcgttgtgaa aacgtgatta cctgctgaag aagcaccacc
     1021 tccacaattt gctgcgatgt cataccagca agccataccg tatatatatt catctgtatc
     1081 taaacagata aaatcgtgag gaaaatggtt agctgcagta ttggttgtat aaatactcgt
     1141 agtaaaacta cctgtagtac aatttagaat cacatttgat aatggaacat cggcagaata
     1201 ggcatctttt gcataatctt gaccaaccca aaatgagtaa tcattggcat tgcccgaaaa
     1261 attggcaact acacgcattt catccggaac atttgatggt ttttgtacaa aatcaaaatg
     1321 ttgtatatca gtaggattag catttggata aactgctaat gtatacgcag cggaaacatt
     1381 ctgagtgcaa caaagaattg atacaaatag taatgcaatt aattgtagta tgtttgtata
     1441 atttcttttc ataataaagg attaaggttt ataatacaaa tgtaatcatt tttatgcaat
     1501 cgttacataa aaacacagta attatacctc tgtaacgtaa aaaatcagta caatattgca
     1561 attaaaccgt tttattttcc ggtccttcta agaaagttgt aatatgtctt tgctttttct
     1621 cttcatacat ttgatctata gggatcataa tgcagcattc aataagttca ccgaattcgt
     1681 gatatacagc aaaaccaaaa gaactcatat tgggagataa gcccatataa gcatttacca
     1741 ggggaggaat attaacaccc aacgctctta cctctctatt aagtatgtgg aaattttctt
     1801 tatatgttgg agcattaaat aaattatcaa tttgttttgg agtaaatgaa atttcaaaag
     1861 gatcaaccgg gtatacaaga ttttcgtgat cgggaaaata ttttctcaaa aatccgaata
     1921 tcatatctct ggctgtagaa ttataatgtt gatatacggt tacttttcca aagaaatact
     1981 taagattggg attttttact aacagagctc ccaaaccatc ccatatatta tccaaagcga
     2041 acaaactctt agcccctttt ttacttgatt gataatcagg ttgtacaaaa gatcgaccca
     2101 attcgatcat gtacggtaag tagtttttaa taaaattgtc ggtaaaataa tacatttcgg
     2161 cagttgccaa atgaggttgt ccattctctg acaattttat atcattagcc attatgtagc
     2221 gataacctcc aataatttct tctgaatccg gatcccaaac aaataattgc ttatatggac
     2281 attccatagt atcaaactca tccatatcaa gagatttacc ggagcctcca cctgcagagc
     2341 gaaaagacaa ctcacgcaat ctagctaatt caagcattat atttggagaa tcttttgcag
     2401 tggtaatata tatttcattt ccacccttat tagtttttct aagaaactta tcgggtgtta
     2461 attctgattt tagtaatttt ctatctacag cttgtataat ttcctccata cacaaattta
     2521 taagttgtac accatggctc gtatttcttg cgccaacacc ttgggagatt ttgtactatc
     2581 gagatttttc catgctacag cttttcctat agttattttt atggttttac ctctttgtct
     2641 atatagctct cgtggaagca tcaacatttc tatattaagt ttcaatccga ttattttgcg
     2701 tatccgtgct acaaaataaa acaaacgcga attttgcgca tcaatacgaa tcggtaacac
     2761 ttgtctatga tactcgaccg actttgatac aaaattcttt ttccattcat catcagcaat
     2821 aattcctttg tgttttctcg acaccctacc tgcagggaaa aacattaatt gcgcatctga
     2881 ggcatatgct gcattcaatc tatctgacaa ttctttgctc tgtccaccaa ctttattaac
     2941 agggataagc atgtccttaa tatttttaag atgcaacaga aaatcatttg caggaattaa
     3001 taaaccctcc gggtatttaa caccaacaac agacacaaca gacactacat ctaccccacc
     3061 taacgggtga ttgcaaacaa acattgttcg attacccggt tccggtaaat tctccaaacc
     3121 cttaacttct aaatgtatat ccatggcctc gactactgcg cacataaaat caacatcttt
     3181 gcgagttcct atcttgagta aaacatcgtt aatccaatct tgatgaatta agcgcttgag
     3241 tatagcaaca actaaacgag aaggtttttt gcccgttcga gatgataaaa ttgatgctac
     3301 atctatcaat gctataccat tcatatccat tctacattta aagtgcaaaa gtacaaattt
     3361 tttaataata atattgaaaa acacctaaaa tatataggat tacacgataa atgaatattt
     3421 ctgtaaataa tgttttattc gttaatgaat tctatttgaa ttttacttaa aattgttgat
     3481 aacttagtgg ataattgtgt cgcaaaaagt tgtaaaatgt cgtaaaatat cttatcttta
     3541 caagtaattt aaaaaataaa aatgccatca tttattggaa atatcgaagc taaagttgat
     3601 gcaaaaggaa gggcttttgt accggctcaa ttcagacgtc tggttgatga aaattcggta
     3661 tttgtactgc gcaagggcat tttcgacgac tgtttagttt tatatcccga agctgtttgg
     3721 gaggaaatta ttggtgttat gcgccaaaaa ttaagtcgtt ggaatagaaa agaagaacaa
     3781 gtattccgcc agtttgtagc cgaggctaat cgtatcgaat tagaagataa tggtcgtatg
     3841 ctgataccta aacgctatat tgagtctatc ggaataaaaa atgacattcg ttttgtaggt
     3901 tgcgatacaa gtattgagat ttggccatca aacaagattg aagaaacctt tatttcagct
     3961 gattcttttg ctgccgagct tgaaaaatta atggaaaagc ccgacaattc gaacaataat
     4021 taactgcgaa caaaattatt gtttgttaaa tttagattta ttcgcattga atagatagaa
     4081 cttatatata ataaaatgtc agaattatac cacactccag tactactaaa cgccagtatc
     4141 gatggcttaa acatcggaaa gggtggcgtt tttatagaca caacattcgg aggaggtggt
     4201 cactctcgcg aaatactaaa taggatggat gcgtcaggca aattactggc ttttgaccaa
     4261 gataaggata ctattgcaaa cataccgaac gattcaagat tcatattcgc acaaagcaat
     4321 tttcgttata taagcaattt tgtaagatac tacaacttat atcctgtaaa tggcatctta
     4381 gccgatcttg gagtatcttc acacgacttt gatgaagctg agagaggatt ctcattcaga
     4441 tttaacggaa aattagatat gagaatgaat cagcgcgcat ttcttacagc tgagacaatc
     4501 atcaatcatt acgatgcaga acatttggct aatgtatttt acttgtacgg agaattaaat
     4561 caatctagaa aaattgctcg tgctattatc aaaaaacgtg agaaaaaagc atttaatgaa
     4621 atatttgaat tagttgattg tgtaaagcca ttctgcggga aagacgaaaa aaaagattta
     4681 gcacgcatct tccaagcttt aagaattgaa gtaaatgacg agatgggtgc actaaaaaca
     4741 atgttattat catctgaaaa agtattagta aaaggaggta ggctttcgat tatcacatat
     4801 cactcgctcg aagacagatt ggtaaagaac tttatgaaga ccggcaattt tgatgggaaa
     4861 caagaaaaag acttctatgg taacctaatc agcccttttt gcacaattgg caaaataaca
     4921 atacccgaca cagaagaaat tattagaaac ccgcgctcac gtagcgcaaa attacgaata
     4981 gcagagaaaa aatgacagat aacagtaaaa aaatcatttc caaaattctt aacaaggatg
     5041 aagttaagga tttaaaagaa acatcaatca aagatgttat tagtggaaat atttttacta
     5101 aaagctattt tctaaagcaa accaaattaa ttctgctcat tatcgcactt gtttttattt
     5161 acatgaacaa ccgtatggtt tgcgaaaagc aattggcact tattgacaaa ctgaacaaag
     5221 aattagaatg cgaaaaatac atttctatgg ttactgaaac tgaactatta agcataagta
     5281 gaccggaaga aattaaaaaa ttggttgaca agcatggttt acaacttgaa cagcctacaa
     5341 tgccccctta taaggtaatt attaaagaat agtggaaaca gcaatataca tagcgctcat
     5401 tttgcttatc gttggtctta ttgtactagc aataatagtt ttaacaagaa agaaggatgt
     5461 taaaacagac atacttgttc gatttggttt tgtatttgtt gttattttag cattattcgg
     5521 gatagtattt ggacgcatta tttacctgca aaatgttgaa ggtgacgctc ttcgtaagtt
     5581 agcagataaa agaatatcta aacctgatac aataaaagca aaaagaggta atattttgtc
     5641 tgacgatggt aggttgatgg ctagctccct tcctacctat tacttgtaca tggatatgcg
     5701 agtagaagcc ttgagaataa taaaaaaagg tgatacaaaa aattatttcc agttacatgt
     5761 taaagagtta gcagatgcac ttgctaaaaa atttggagat aagacagcca aagaatacga
     5821 aagagatttg accagagcat ataaaaacaa aagcgctcaa tacaaaatat atccaaaacg
     5881 agtatcgtat ttggattaca tggaaattaa aaaattccct attttgcata ggggttcgat
     5941 tcaaggggga ttttttactg aagaacgcgt aacaagggta aagccattcg gttcacttgc
     6001 atcaagaaca ataggtgata tttatggtat agaagaaaaa ggcggtaaaa atggtttaga
     6061 gttacaatat gatagtgttt tgcgaggtac agaaggtctt aaggtaactc gcaaaatggc
     6121 agggaaacgt acagatatta ttttaaaaga tccaataaac ggaattgacg ttgtaagcac
     6181 gattaacatt gacctacaag aagctgcaga aaaatcttta cgaaatgaat taacagaatg
     6241 tcaggcatct tgcggaactg ttgttttgat ggacgtaaaa acaggagctg taaaagcaat
     6301 ttcaaatttg acaaggaacc caaatggtac atatagtgaa gttactaaca tcgctgttgc
     6361 aagtcagatc gaaccgggat ctacttttaa aactttatct ttattagtcg cattggaaga
     6421 tggtgttata gatacgacta caatgattaa taccgagaat ggcggtcatc agttcgcaaa
     6481 cagaattatg aaaga
//
LOCUS       DAOPID010000073         6316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_16063_length_6316_cov_4.020444, whole genome shotgun sequence.
ACCESSION   DAOPID010000073 DAOPID010000000
VERSION     DAOPID010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 6316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6316
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16063_length_6316_cov_4.020444"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..1092)
                     /locus_tag="PK641_03645"
     CDS             complement(109..1092)
                     /locus_tag="PK641_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HPG55477.1"
                     /translation="MRVKLLIAFFACVFCLTAQDLSVYGKLDPAQIQIGEQTTLRYEF
                     AQPKDTKVSMPLLQDTIAKGVEIVEVLKPDTIDLGNGRIQINLDYIITSFDSGFYFIP
                     AMQFASANEKIESRPIGLTVHTVKVNPETDSVKDIKEIMDAPFSWKELFKWVGIVLGI
                     LAVIALIAFILIKYVFKKKVPFIPVKEEIKESPDVVALRRLEQIKEEKLWQSGKIKEF
                     YTELTEVVRVYIASRFDINAMEMTSEEIMALTRKHPDMDEVRTILRQMLELSDLVKFA
                     KFIPLDKENYRSIIDAFTFVEKTKVTEPEIVSDDAENPKNTDETVGKESKS"
     gene            complement(1097..1963)
                     /locus_tag="PK641_03650"
     CDS             complement(1097..1963)
                     /locus_tag="PK641_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPG55478.1"
                     /translation="METSEILKKVRQVEIKTRGLSQNIFAGQYHTAFKGRGMAFSEVR
                     EYQFGDDIRNIDWNVTARFNHPYIKVFEEERELTVMLLVDVSGSGDFGTERSTKREMM
                     TEIAATLAFSAIQNNDKIGVIFFSDKIEKFIAPQKGKKHILYIIRELIEFRPESRKTD
                     ISVVLRYLTNAIKKRCTAFLISDFISDNFDKALMIAGKKHDLVALQVYDQKDSEMPDI
                     GLMKVKDSETGECMYVDTSRWDVRKSYEEHWRKRQELMSKEFSKNGIDLASVRTCDDY
                     VKVLINLFRMRG"
     gene            complement(1989..2984)
                     /locus_tag="PK641_03655"
     CDS             complement(1989..2984)
                     /locus_tag="PK641_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPG55479.1"
                     /translation="MGQVVDIRELNARIEKQSAFVDVLTLGMDKVIVGQKHLVESLLI
                     GLLSDGHILLEGVPGLAKTLAIKTLAQLIDSKYSRIQFTPDLLPADVLGTMIYSQKTE
                     EFIVKKGPIFANFILADEINRAPAKVQSALLEAMQERQVTLADSTYKLDEPFLVLATQ
                     NPIEQEGTYPLPEAQVDRFMLKVIVSYPKQEEERLIIRQNIGPKQAPVIPVLKASEII
                     AAREVIREIYLDEKIEKYILDIVFATRDPKLYNLDDLQGMISFGASPRASICLALAAR
                     SYAFIKHRGYVIPEDVRAVCYDVLRHRIGLSYEAEANNLTSEEIISRILNIVEVP"
     gene            complement(3166..4203)
                     /locus_tag="PK641_03660"
     CDS             complement(3166..4203)
                     /locus_tag="PK641_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPG55480.1"
                     /translation="MENKKISIQELTDIIAKESNSTKKFSEDFIRELVDVIAEYLEKD
                     GIVKVKGLGTFKLLWIEDRKSVNVTTGETIILPARYKVSFIPESSVKQSINAPYSHLE
                     TFVPEPPQHVDNNGYKKIAEEPIEISVEQSTEKSEEPITEPAASKQDVKNSESSSRPE
                     KKQKRNITWLIILIVLLLLCGATFYFQAMWVPIARTNIEKWFGIKIEPDTVAVAPVEL
                     EENLLLEDSTELYFDSLYNQSDSLVTDTVAIAEMAPIADYNPSVAKDAPVRETVIVRD
                     GSRLTMVAYRAFGHKAFWVYVYDANRDVLRHPNDVAQGMRLRIPDLDPSLVDPNNEEC
                     IKKAIEIGKKY"
     gene            complement(4193..4486)
                     /locus_tag="PK641_03665"
     CDS             complement(4193..4486)
                     /locus_tag="PK641_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPG55481.1"
                     /translation="MNNKELLVELSNRLGKTQKEVSAMLDVTTDCMVRSFTENSNLVL
                     QGFGSFEVRKKADRILVNPTTKLQMIVPPKLALIFKASNTYKDKIKNKEHNGK"
     gene            complement(4529..5830)
                     /gene="rimO"
                     /locus_tag="PK641_03670"
     CDS             complement(4529..5830)
                     /gene="rimO"
                     /locus_tag="PK641_03670"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HPG55482.1"
                     /translation="MRKNHIDIISLGCSKNLVDSEALMRQFEAVGYTVSHDPEHPNGD
                     IAIINTCGFIGDAKEESINMILRFCQRKQSGQLERLYVMGCLSQRYLEELKIEIPEVD
                     KFYGKFDYNGIIADIGKEYRQDLRLERHLTTPKHYAYLKISEGCNRTCSYCAIPIITG
                     KYKSRTIEDIEQEVRWLVSKGVKEFQVIAQDLTYYGYDIYKDYKIADLISRLSDIKGV
                     EWLRLHYAYPTNFPYSLLTVMREKDNVCNYLDIALQHISDPMLKKMRRSITSDETYDL
                     LARIREEVPGICLRTTLMVGHPGETEADFNQLKEFVQKVKFDRMGAFKYSDEEKTYAN
                     LHYKDDVPLDVKQDRLDELMNIQQSISFDLNQSKIGQVWKVIIDREEGDYYVARSEFD
                     SPEIDMEILIPKDENSLSIGNFYKVKIIRAEEFDFYAKIQK"
     gene            complement(5835..>6316)
                     /locus_tag="PK641_03675"
     CDS             complement(5835..>6316)
                     /locus_tag="PK641_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPG55483.1"
                     /translation="EQLDIWGERVGVPVIKQKMGADPASVAFDTLSSAKANHADIVII
                     DTAGRLHNKINLMNELTKIRNVMQKVVPDSPHEVLLVLDGSTGQNAYEQAKQFTKATQ
                     INALAITKLDGTAKGGVVIGISDQFKIPVKYIGLGEKMEDLQLFDKKAFVESLLRKK"
BASE COUNT         1949 a         1259 c          900 g         2208 t
ORIGIN      
        1 ccgatgaaat tttaacttcc ggtgtaaacg attttcgttt ccaaacgtac catgcaacta
       61 ctggcactac caatagcaac aacaaaaaat aatatggatt agcgaatgtc atgatttaga
      121 ctcctttcct acagtttcat ctgtattttt tggattttca gcatcatcac taacaatctc
      181 aggttctgtt actttggtct tttcaacaaa agtaaacgca tctattatag aacgataatt
      241 ctctttatca agaggaataa attttgcaaa cttcactaaa tcagacaatt ctaacatctg
      301 tcgcaaaatt gttcttacct catccatatc aggatgtttt ctggtaagtg ccataatttc
      361 ttcagatgtc atttccattg catttatatc aaaacgacta gctatataaa cacgtacaac
      421 ttcagtcaat tcggtataaa attctttaat tttgcctgac tgccataact tttcttcttt
      481 tatctgctct aaacgacgta aagcgactac atcaggagat tctttgattt cctcttttac
      541 cggaataaat ggcaccttct ttttgaaaac atatttaatt aaaatgaatg ctattagtgc
      601 aataactgct aatataccta acacaatgcc cacccattta aaaagttctt tccaactaaa
      661 tggagcatcc atgatttctt ttatgtcttt aacagagtct gtttcaggat ttacctttac
      721 agtatgcaca gttaaaccta ttggacgact ttctattttt tcgtttgcac tagcaaactg
      781 catcgcagga ataaaataaa aaccactatc aaatgatgtt attatgtagt ctaaattaat
      841 ctgaattcta ccattgccta aatcaattgt atccggcttt aaaacttcta caatttctac
      901 tccttttgct atagtatcct gcaacaatgg cattgatact tttgtatctt tgggctgggc
      961 aaattcatag cgaagcgtcg tttgttctcc tatctgtatt tgtgcaggat ctaacttgcc
     1021 atacactgac aaatcttgtg ctgtaagaca aaaaacacac gcaaaaaatg ctattaatag
     1081 ttttactctc atataattat cctctcattc taaacaaatt aattaaaacc tttacgtaat
     1141 cgtcacatgt tctaacagaa gccaagtcaa ttccattttt ggaaaattct tttgacataa
     1201 gttcttgacg tttacgccaa tgctcttcat aactttttct aacatcccat ctggaagtat
     1261 caacatacat acattcgcct gtttcagagt cttttacctt catcaaacca atatcaggca
     1321 tttctgaatc tttttggtca tacacttgca gagccactaa atcatgtttt ttaccggcta
     1381 tcataagtgc tttatcaaaa ttatctgaaa taaaatccga gataaggaat gctgtacaac
     1441 gttttttgat cgcatttgtc aaataacgca aaaccacaga tatatctgtt ttcctacttt
     1501 cgggacgaaa ttcaattaat tcacgtatta tgtataggat atgtttctta cctttttgag
     1561 gtgctataaa tttttctatt ttatcagaaa aaaatataac acctatttta tcattatttt
     1621 gaatggctga aaatgctaaa gtagcagcta tttctgtcat catttcacgt tttgtactgc
     1681 gttccgtacc aaaatcacca ctacctgata catcaaccaa taacataacg gtaagttctc
     1741 gttcttcttc aaatacttta atatatggat gattaaaacg agctgtaaca ttccaatcaa
     1801 tatttctaat atcgtcaccg aattggtact cacgtacctc ggaaaaggcc atacctctac
     1861 ccttaaaggc agtgtgatac tgacctgcga aaatattctg cgacaacccg cgcgttttaa
     1921 tttcgacttg ccttactttt tttaatattt cgcttgtttc catagaaata aattgatttt
     1981 tcccttcatt aaggaacttc aacaatattc aagattcggc ttataatttc ttctgatgtt
     2041 aaattatttg cttctgcttc atagctcaaa ccaattctgt gacgaagaac atcatagcat
     2101 actgcacgaa catcttccgg aataacatat cctctatgtt taataaatgc ataagaacga
     2161 gctgccaaag ccaaacatat tgatgcacgt ggagaagctc caaatgaaat cattccttgt
     2221 aaatcatcaa gattatacaa ttttggatca cgagttgcaa atactatatc taaaatgtat
     2281 ttctcaattt tttcatccaa atatatttct cttataactt cgcgagctgc tataatttca
     2341 gatgctttca aaacagggat tacaggagcc tgtttgggtc ctatattctg acgaataata
     2401 agcctttctt cttcttgttt tggatagcta acgattacct ttaacataaa acggtctact
     2461 tgagcttcag gtagtggata tgttccttct tgctcaatag ggttttgtgt agcaagaact
     2521 aagaatggtt catccaactt atatgttgaa tctgctaagg ttacttgacg ttcttgcata
     2581 gcctcaagta aagcactctg aacttttgca ggagcacgat ttatttcatc tgctaaaata
     2641 aagttagcaa atattggccc ctttttaact ataaactctt cggttttctg actataaatc
     2701 atagttccta atacatctgc aggcagcaaa tccggagtaa actgaatacg gctatattta
     2761 ctatctatta gctgtgctaa agtcttaatg gctaatgttt ttgccaaacc gggcacacct
     2821 tctagcaata tatgtccgtc agaaagaagt ccgattagca atgactcaac caaatgcttc
     2881 tgacctacta tgactttgtc cattcctaag gttaggacat caacaaatgc actttgtttt
     2941 tcaatgcgtg catttaattc tctaatatca actacttgac ccataactta tgatatgaat
     3001 taatgtataa taaaaaaaac accgctgaac aaattcagca gtgcgaagat atgcaaatgt
     3061 taaaatgatt ccaacattta tgccgttaaa aacgtttaag cgaatgttat aaatggtaga
     3121 tatatgccct gaatagtcta aaatgcttaa ttttttgcaa acaaatcaat attttttacc
     3181 aatttcgatt gcctttttga tacattcctc gttatttgga tcaacaagtg acggatccaa
     3241 atcaggtata cgtaatcgca ttccttgagc aacatcatta ggatgcctta gcacatctct
     3301 atttgcatca taaacatata cccaaaatgc cttatggcca aacgcacgat atgcaaccat
     3361 cgttaaacga ctaccatctc ttactattac agtttctcta acaggtgcat ccttagcaac
     3421 cgatggatta taatcagcta ttggtgccat ttccgcaata gctactgtat ctgtaaccaa
     3481 agaatctgat tgattatata atgaatcaaa atacagttct gttgaatctt ctaatagtaa
     3541 attctcttcc aactctaccg gagctacggc aactgtatca ggttcgattt taattccaaa
     3601 ccatttttct atgtttgtac gcgcaattgg cacccacatt gcttgaaaat aaaatgtagc
     3661 accacataat agcaataata ctatcaatat tattaaccat gttatattac gcttttgttt
     3721 tttctcaggc cttgaagatg attctgaatt ttttacatct tgttttgatg ctgcaggttc
     3781 tgtaataggt tcttcagatt tttctgttga ttgttcaaca gaaatttcta ttggctcttc
     3841 tgcgattttt ttatagccat tattatctac atgttgagga ggctccggca caaaagtttc
     3901 taaatgcgaa tatggagcat tgattgattg ctttacagat gattcaggta tgaacgagac
     3961 tttatagcga gccggtaaaa tgatcgtttc tccggtcgtt acatttacac tcttacgatc
     4021 ttcaatccac aataatttga atgttccaag acctttaacc tttacaattc catctttctc
     4081 taaatattct gctataacat caaccaattc gcgaataaaa tcttcagaaa actttttggt
     4141 tgaattagat tccttcgcaa ttatatcggt tagctcttgt attgatatct ttttattttc
     4201 cattgtgttc cttattttta atcttatcct tgtaggtatt acttgcctta aagattaacg
     4261 ctaacttagg aggtacaatc atttgaagtt ttgtggtagg attaacaaga atacgatcag
     4321 cttttttacg tacttcaaaa gacccaaaac cttgtaatac aaggtttgaa ttttctgtaa
     4381 aggaacgaac catgcaatct gttgttacat ccaacatagc agatacctct ttttgagttt
     4441 ttcctaaacg atttgatagt tctactaata attctttgtt attcacatac ttagatttaa
     4501 gaagtgtcta taagcgcaaa tatatgaatt atttctgaat tttagcataa aaatcaaatt
     4561 cttcagctct aattattttt actttgtaaa aatttccgat ggaaaggctg ttttcatcct
     4621 ttggaatcaa tatttccata tctatttcag gtgaatcaaa ctccgatctg gcaacataat
     4681 aatcaccttc ttcgcgatct attattacct tccaaacttg ccctatttta gactgattca
     4741 aatcgaacga gatagattgt tgaatattca tcaattcgtc taaacgatct tgctttacat
     4801 caagtggaac atcgtctttg tagtgtaaat tggcataggt cttttcttca tcagaatact
     4861 taaatgcgcc cattctatcg aattttacct tttgcacaaa ctctttcaat tgattaaaat
     4921 ctgcttctgt ttcgcccgga tgtccgacca ttaatgttgt gcgcaaacaa attcccggaa
     4981 cttcttccct tatacgtgca agcaagtcgt atgtttcatc agaagtaatg cttcgacgca
     5041 tcttcttaag catcggatct gaaatatgtt gtagtgcaat atctagataa ttacaaacat
     5101 tatctttttc gcgcatcaca gtcaataaag aatatgggaa atttgtagga taagcataat
     5161 gcaagcgtaa ccactctact cctttaatat cagacaaacg agatattagg tcggctatct
     5221 tataatcctt gtaaatatca tagccataat atgtcaaatc ttgagctatg acctgaaatt
     5281 ctttaactcc tttagaaact aaccaacgaa cttcttgttc tatatcttca attgtacggc
     5341 ttttatattt acctgtaatt atgggtattg cacaatatga acatgtccta ttacagcctt
     5401 ctgaaatttt aaggtaagca taatgttttg gtgtagtaag atgcctctct aacctcaaat
     5461 cctgacgata ctccttgcct atatcagcta ttattccgtt ataatcaaat tttccataga
     5521 atttatctac ttcaggaatt tctattttta attcttccaa ataacgttgc gacaaacaac
     5581 ccataacata taaacgttcc aattgacctg attgtttacg ttggcaaaaa cgaagaatca
     5641 tatttatact ttcttcttta gcatctccga taaatccgca ggtatttata attgcaatat
     5701 cgccatttgg atgttcagga tcatgcgaaa ctgtataacc tacagcctca aattgacgca
     5761 taagagcctc actatcaacc aaattttttg aacaacctaa actgattata tcaatgtgat
     5821 ttttgcgcat aaaactattt tttacgtaga agtgattcga caaaagcttt cttatcaaac
     5881 aactgtaaat cttccatttt ttctcctaaa cctatgtatt taacaggaat tttaaactga
     5941 tctgagattc ctattacaac accaccttta gccgttccat caagttttgt tatggccaaa
     6001 gcatttattt gagtagcttt tgtgaattgt tttgcctgtt cataagcatt ctgtcctgtt
     6061 gatccatcta atacgagtag aacctcgtga ggagaatccg gaacaacctt ttgcatcaca
     6121 ttacgtattt tggtcaactc gttcatcaaa ttgattttat tatgcagacg ccctgctgta
     6181 tcaataataa caatatctgc atgatttgct tttgcagatg acaatgtatc aaatgcaaca
     6241 gatgcaggat ctgcacccat tttttgttta ataaccggaa ctccaaccct ttcgccccat
     6301 atatctaatt gctcaa
//
LOCUS       DAOPID010000074         6207 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_16487_length_6207_cov_3.010566, whole genome shotgun sequence.
ACCESSION   DAOPID010000074 DAOPID010000000
VERSION     DAOPID010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 6207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6207
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16487_length_6207_cov_3.010566"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(369..2738)
                     /locus_tag="PK641_03680"
     CDS             complement(369..2738)
                     /locus_tag="PK641_03680"
                     /inference="COORDINATES: protein motif:HMM:NF027798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,3-glucanase family protein"
                     /protein_id="HPG55484.1"
                     /translation="MKKRLLLLFMAISLLSITCIGKSLPIQLVNNSNFPDNQVYIAVI
                     GQHSDVGNMWINLAANTQWDAQMKGMSASQNTLHKTNGDWGYGNIFYKLSDIKDKTIY
                     IPEGAFGGRIFFSFGSPLYIHFHAEGGYAGVDMRNPNDPNKGIRWEIIEYTYFEPARS
                     GLFMNTSRVDAFQYPMGLEIIGDGNGANFSQYQKVGELMSYAQVIAKWNQQLGNSDYN
                     VCLQDVITTDNLGCIIEQPSKVDAFKTTASFSEFNAYVNQIWDAFQSKTLNAGHGERG
                     NWSGNASGNTFTMSNPSWGGGEKTCHIYSRPSATDCIEGAGAFANGSIGDKALQAQFC
                     AAINRGIINTNLGSNVFQDWWDESSFFPAGKCYNKYVWFFHQKEISNNSRTYAFAYDD
                     VANQSATLQCTYPEKAVITIGGFTEDPGAIGNIDNDEVAFHDLPTSMESSATYTVSVD
                     YTASTSREIWICLFDNTGTWTPVVGGENKVTVPEGSGTVEISINPSVAPAIGKGYTWK
                     CDIRPVGGAWDTFLDDDIIDNISITTPSQKETYNYWVVLRDNVSDMDSYLDLRSNEFA
                     EWEGTVSQIVAGQYEGNDALAYNVNSAVGSWFGFGVVNTSGSYYFGDLADYGLHFAYK
                     TDYNGPLSVKMGGTNGEFSVEFTPIADNKWHSQEISMSEFIDAGLELGTTTDNLIFSI
                     ISENVVKTNASINVDDIYYYKTDVLPVILTGADIVETNDLSVYQPNADLLCINGTSKG
                     SQIELINMNGISMTKTEATEGTSYLSINSMPSGLYLLRVDSKVIKVLIK"
     gene            complement(3290..5857)
                     /locus_tag="PK641_03685"
     CDS             complement(3290..5857)
                     /locus_tag="PK641_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HPG55485.1"
                     /translation="MKQITTLFSYLLVITCFLFIDCHVSMAESGVYVGGHIRRERPNT
                     ITKLKNSGFTYVILFNVNVESDGTLKTDGETICQNGQYVFGNTQPNYQADIKALKTSP
                     THINRIEICIGGWGNESYDHIKTLINNNGTGSETMLYKNFKALKNAIPELDGVNNDDE
                     NCYDLSTATRFHVMMKDLGYKTSLAPYMNKDFWSQLATNINNQRSNAVDRIMVQCYDG
                     GAGNNPSNWHINGITLHAGRMNYQDGGMSGSINQFQSWKNDNGVTGGFVWVYNDETWD
                     LNAWATRMNRVFGSCNSATNPVATVYEGANYEGYSKQLAEGNYTMADLAAYGITNDDI
                     SSIKISTGFKITLYDNDKYGGSTASFTSDATFVGSDRNDKCTSLKIEPSGVTDISGIY
                     KIKNRNSGLYLDMAGNGTENGTNVVQYNDEGEEAFQLYEFKHKGNGVYTITCKGNGKV
                     LDIKESKSDNGTVVQAYTSNDTKAQQFILVDKGSGYYQIIARNCGKPIEVPGSSKQAG
                     EWIKIYDNNGTNAQQWQLIKLKPIGVAVASIYNDLNYAGTSLSLPEGSHSLNQLKIYG
                     FADNSLTSLKVTKGYKVTIYVDDNYKGSSKSFTSDVNWIGDDWNDKTSSIKIEAQGIS
                     GLNGEYKIQNKNSSLYLDLYENKTDNNTAIVQWNDLGKSETQKFKLVERDNGVYSIYS
                     APANRVFDVANASVNDRANIQLYDYYADAHNQQFMICDAGNGYYQFIARHCGKVIEVP
                     ESDKNAGEWIKTWSNNGSAAQAWKLVPWSQVITTQINSTSNTENISIYPNPACNYINI
                     KWANYAKRTIYLKDLEGRILCNTNCESNILSIPITEIQNGIYLLIIDNQSYKILVKH"
BASE COUNT         1817 a         1103 c         1024 g         2263 t
ORIGIN      
        1 taaaaacgtt ttcctgagca ctggtcaaaa atgtaaatca gcctttgtcc ttttctgcat
       61 aaaacagtta taatcaacaa tatagatgta tttttgcata atgcaatgtg gattaaaaca
      121 tacttcacaa ctgtacttca aatactattt ttaattcgtt tatttacgat taatgccttt
      181 tgtaagctac ccgattttag tacagtttta aattattttt tttcttttaa gatttgtgta
      241 ttttgcatac cgaaacggct gtttcgaaaa tgtaagtttc ggaacatatt caaattgaaa
      301 tattaaacta cacccctaaa gttttttatc taaccttagg ggtgtatttt aatattcagc
      361 ttttgatctt atttaataag aaccttaatt accttactat caacacgtaa taaatataat
      421 ccggaaggca ttgaatttat cgacaaatag ctagtaccct cagtagcctc agtctttgtc
      481 atactgattc cattcatgtt tattagttct atttgtgaac cttttgatgt gccattaatg
      541 catagcaagt cagcatttgg ttgatataca gatagatcgt ttgtttcgac gatatctgct
      601 cccgtaagaa ttacaggtaa tacatcagtt ttgtaataat aaatgtcatc aacatttatg
      661 cttgcattag ttttaactac attttcacta ataatagaga atatcagatt gtctgttgtt
      721 gttcctaatt caagacctgc atcaataaat tcggacatgc tgatttcttg actatgccat
      781 ttgttatccg caataggagt gaactcaacc gaaaattctc cattagtacc acccattttg
      841 acagacaatg ggccattgta atctgtttta taagcaaaat gtaagccata atctgcaagg
      901 tctccaaaat aataagaacc acttgtgttt actactccaa aaccgaacca agatccaaca
      961 gctgaattaa cattatatgc tagagcatcg tttccttcgt attggccggc aactatttga
     1021 cttacagtgc cttcccattc tgcaaattcg ttacttctta aatctaaata agagtccata
     1081 tctgatacat tgtcacgaag aacaacccag taattatatg tttctttttg agatggtgtg
     1141 gtgatgctta tattgtcaat aatatcatca tcaagaaatg tatcccatgc tcctccaacc
     1201 ggacgaatat cgcatttcca agtataacct ttgccgattg caggtgctac gctagggttg
     1261 atacttattt caacagtacc actgccttca ggaactgtaa ctttattctc gcctcctaca
     1321 acaggggtcc aagtgcctgt attatcaaac aaacaaatcc atatttcgcg agatgtagat
     1381 gctgtataat ctacagacac ggtgtaagtt gctgaacttt ccatgctagt tggaaggtca
     1441 tgaaatgcta cttcatcatt atctatattg cctattgcac ccgggtcttc tgtgaatcca
     1501 ccgattgtaa taacagcttt ttcaggatag gtacattgta acgtagcgga ttgattagcg
     1561 acatcatcat atgcaaatgc atatgtacga ctattattgc taatttcttt ttgatggaag
     1621 aaccaaacgt atttattata gcatttgcct gcagggaaaa agctgctttc atcccaccaa
     1681 tcttgaaaga cattacttcc taagtttgta ttaattatac cgcgattaat agctgcacaa
     1741 aattgtgctt gtaatgcttt atcaccaata cttccatttg caaatgcgcc ggcaccttct
     1801 atacaatctg ttgctgatgg gcgcgaataa atgtgacaag ttttctcacc tccgccccac
     1861 gatgggttac tcattgtaaa agtattaccg gaagcattac cagaccaatt gccgcgttcg
     1921 ccatgacctg cgtttagtgt tttactttga aaagcatccc atatttgatt aacgtaagcg
     1981 ttaaattcag agaaactagc tgttgtttta aatgcatcaa cttttgatgg ttgttctatg
     2041 atacatccta agttatctgt tgtaataaca tcctgaaggc aaacattata atctgaatta
     2101 cctaattgtt gattccattt tgcgataact tgagcgtatg acattaattc accaactttt
     2161 tggtattgcg agaaattagc accatttcca tcgccaatga tttccaagcc catagggtat
     2221 tgaaaagcat ctacgcgcga cgtgttcata aatagacctg atctagccgg ttcgaaatat
     2281 gtatattcaa ttatttccca acggatgcct ttattagggt cgtttgggtt acgcatgtca
     2341 acaccggcgt atcctccttc agcatggaaa tgtatatata aaggagatcc gaaactgaag
     2401 aatattcgac ctccgaatgc accttcgggt atataaattg ttttgtcttt tatatcactt
     2461 aacttataaa aaatattgcc atagccccag tccccatttg ttttgtgcag agtgttttga
     2521 gaagcactca tacctttcat ttgtgcatcc cattgagtgt ttgcggctaa gtttatccac
     2581 atatttccaa catcactatg ttgacctata actgctatgt aaacttgatt gtcaggaaag
     2641 tttgagttat taaccaattg tattggtaaa ctttttccga tacatgtaat ggacaataaa
     2701 cttattgcca taaaaagtaa taataatctt tttttcattg tgttgattta ttaatggttg
     2761 tgagagcaaa attagatatg ttttaatgcc attaatcgct catgaatcag tccaagaaag
     2821 taatgtaagt tttgaaaaat acgtctttgt tttaaatata ctgtaaatca gacgattaat
     2881 acaaatatat gaaattcata aagtagttat aacgaacttt ctcaacagta catcaaatac
     2941 gcattttgtc gaaaattgtt gctgttatga tggtaaaatt tataatgctt tagttgatat
     3001 gcttcttggt atatttccta ttttggcaac tttttgatgc ctttataagg atttcagtaa
     3061 gagtgtaaaa gatatagatg gttttgtaga aggtgattgt agcagcagag gtaaatgcga
     3121 aggccttgga atggcaatat atataataat tctttgcgtt cttattttct gtatggtgca
     3181 gtttgctatg tttgtagaat gattgctggt ctgtttaaat ttattagttt gttgggtgga
     3241 agtaaattcc acccacaaac taactcaaaa caattacaac aacacgatat tagtgtttaa
     3301 ctaatatttt atagctttga ttgtctatta ttaagagata aataccattt tgtatctctg
     3361 taataggtat agacaatata ttagattcgc aattggtatt gcataatatt cttccttcta
     3421 aatcttttaa atatatagtt ctttttgcat aatttgccca tttaatatta atgtaattgc
     3481 aagctggatt tggatatatg cttatatttt cagtattgga agtagagttt atttgtgtag
     3541 ttataacttg cgaccaagga actaatttcc atgcttgagc tgccgatcca ttgtttgacc
     3601 atgtttttat ccattctcct gcatttttat cgctttccgg aacttcaatt acctttccac
     3661 agtgccgtgc aataaattgg taatatccat tacctgcatc gcatatcata aattgttggt
     3721 tatgtgcatc tgcgtaataa tcgtatagtt gtatgtttgc tctatcgtta acggatgcgt
     3781 ttgcaacatc aaatactctg ttggctggcg cactgtatat gctgtaaacg ccattgtctc
     3841 gttctactaa cttaaatttt tgagtttctg attttcctaa atcattccat tgtacaatag
     3901 cagtgttatt gtctgttttg ttttcgtata aatcaagata cagagagcta tttttatttt
     3961 gtattttgta ttcgccattt aagccagata tgccctgtgc ttctattttt atagatgatg
     4021 ttttatcgtt ccaatcatct ccaatccaat ttacatcaga tgtaaaactc tttgaagagc
     4081 ctttatagtt atcatcaaca taaatagtaa ctttgtatcc tttggttact tttagtgatg
     4141 ttagtgaatt atcggcaaaa ccatatattt ttaattgatt aagcgaatgt gaaccttcgg
     4201 gtaaagacag tgaggttcct gcatagttaa ggtcattgta tatgcttgca actgcaactc
     4261 ctattggttt tagtttaatc aattgccatt gttgtgcatt tgtgccattg ttatcgtaaa
     4321 ttttaatcca ttctcctgcc tgtttgctcg aacctggtac ctcaataggt ttgccgcagt
     4381 ttcgtgctat aatttggtaa taaccactgc ctttatcaac caaaataaat tgttgagctt
     4441 ttgtgtcgtt gcttgtatat gcttgaacta ctgttccatt gtcgcttttc gattctttta
     4501 tatctaaaac ttttccgttg cctttacaag taattgtata tacgccattt cctttatgtt
     4561 taaattcgta tagttgaaag gcttcttcac cttcgtcatt gtattgaact acattggttc
     4621 cgttttcggt accattgcct gccatatcaa gatatagtcc gctatttctg tttttaatct
     4681 tatatattcc agaaatatca gtaacgccag atggctctat ttttaaagat gtacacttgt
     4741 cgtttctgtc tgaacctaca aatgtagcgt cagaagtaaa agatgcagtg ctgccgccgt
     4801 atttgtcgtt atcgtataaa gtaattttaa atcctgtgct tatcttaatt gaagatatat
     4861 cgtcattagt aatgccgtaa gcagcaaggt ctgccatagt atagttacct tctgcaagtt
     4921 gtttagaata tccttcataa tttgcgcctt cgtaaacagt tgcaacgggg ttggttgcag
     4981 aattacacga accgaaaact ctgttcatac gtgtagccca tgcattcaga tcccatgttt
     5041 catcattgta aacccataca aagccaccgg taacgccatt gtcgtttttc catgactgaa
     5101 attgatttat ggaaccagac attccaccat cctggtagtt cattctgcct gcatgcaggg
     5161 taattccatt aatatgccag tttgaaggat tattgcctgc acctccatcg taacactgaa
     5221 ccataattct atcgacagca tttgaacgtt gattatttat gtttgtagcc aattgcgacc
     5281 aaaaatcttt attcatatat ggtgcaagtg aagtcttata tcctaaatct ttcatcataa
     5341 catgaaatct tgtggcagta ctgaggtcgt agcaattctc atcgtcatta tttactccat
     5401 caagttctgg aatagcattt ttaagtgctt taaagttttt gtaaagcata gtttccgaac
     5461 ctgtgccatt attatttatt aatgttttaa tatggtcgta cgattcattt ccccagccgc
     5521 caatacatat ttcaattcta tttatatgtg taggtgaggt ttttaatgct tttatatctg
     5581 cttgatagtt tggttgagtg tttccaaaaa catactgacc attttgacat attgtttcgc
     5641 catctgtttt taaggtacca tcgctttcta catttacatt aaacaaaata acgtaagtaa
     5701 aaccggagtt ttttaatttg gttatagtat taggtcgttc gcgtcttata tgaccgccta
     5761 catatacgcc actctcggcc atacttacat ggcaatctat aaataaaaag catgttataa
     5821 ctaataagta actgaacagg gtagtgattt gtttcatatt gtgtgatttt gggtgtattt
     5881 tgattctgca ttacaaagaa atatataaag attgatttaa taaacaggac agatatatac
     5941 agtaataatt tggaatatat ttaataaaaa tgcaatgctt gtttgtgtaa gtgtcagtat
     6001 attaaggtgt tattattaac tgaatttttt attgattgta gatttgttag agtaaaatta
     6061 agcgacatag ctacctgaat cagggtttaa atatgccgtt taatttaata atttatgttg
     6121 ccttatttaa cttgtaattt aacagattta tcgcctgttt ttataatata caaaccttcg
     6181 tttaaacctg ttacatctaa agtagta
//
LOCUS       DAOPID010000075         6133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_16764_length_6133_cov_4.432215, whole genome shotgun sequence.
ACCESSION   DAOPID010000075 DAOPID010000000
VERSION     DAOPID010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 6133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6133
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16764_length_6133_cov_4.432215"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2161)
                     /locus_tag="PK641_03690"
     CDS             complement(<1..2161)
                     /locus_tag="PK641_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPG55486.1"
                     /translation="MKKLSLICAVFFVLTANLHAQSFTGEQALQGYGVFGDQLTFIFD
                     EALYGVTPKKVFVTGEFRNWDTSINSPEWNLKQTGQQWLLTIDNTDFAIVKPNTKFKF
                     RTDNGDWMSPPSGAPNQKGGDLVFMKQDESLELKAEIKSDNLIWAEIGANRSFLPSDY
                     VISDAQGNKIKVAKVLPNGAKTTLIVPESPLDKRRAYYLEIPSQNQKVICSYDGWFRE
                     LCSSKEMGANIDNGKTTIRVFSPRAEKVKLYLYKNKDDDKAYRIEEMKQDKDGVWESF
                     FNEDLHGVYYDFTVHGSKDPGNLFYETRPVHVNDPYARVSDDTFGKCRIWHRTVPAKP
                     LKNGIPTMENVIAYEVHVQDFTDKLPLPDSLKGTLKGMVASGLKNENGKKIGFDYLIE
                     LGINTVHLMPVQEYLHFPTDDWKASFENDPYMKEQGVSEENYQWGYRTSFAMAVESRY
                     RTKGSELGSERNQFRDLVQAFHDKNIAVIIDIVPNHTAEDMDGQSWNFNFNGFDKQYY
                     YRTKNFEHIGGYGNEIKTENRPMTQRWLIDQCLYFINEFGIDGFRIDLAGQIDQQTLL
                     ALREIVGKDKIIYGEPWIGSNDPNYENNPDWDWYKEDSPITYFQDEVRTAYKGPVFEL
                     KDPKNDRGWSGGKYSERPNVMKGLTNSYKDDKTPNSGISYLDIHDNFALADQFGKNFD
                     GRTDVYQDEYKIAVTLLYTTLGPIVLHGGSEIMRSKAH"
     gene            2260..3075
                     /locus_tag="PK641_03695"
     CDS             2260..3075
                     /locus_tag="PK641_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HPG55487.1"
                     /translation="MEQLNQLWENLITSGIDLGKSIIAASVIYFVGRYIVKLINKVFN
                     KMMERRKLDPAVKSFLGSLVNITLTILLIIAVISALGIETTSFAALLASAGVAIGMAL
                     SGNLQNFAGGLIILLFKPYKIGDVVETQGKTGLVKEIQIFHTIITTADNKTIFIPNGS
                     ISSGVLTNFSDQDLRRVDWSIGIEYGEDYEKAKAAIEELLNADKRILKTPEYFIALEQ
                     LADSKVNIAIRAWVKSADYWDVFFDFNKTVYSEFNKKGIGFPFPQITVHQSKK"
     gene            complement(3134..4969)
                     /locus_tag="PK641_03700"
     CDS             complement(3134..4969)
                     /locus_tag="PK641_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HPG55488.1"
                     /translation="MKVCIAEKPSVAKDIAQVLGATKRCDGYIEGNGYQVTWTFGHLC
                     TLKEPHDYKEEWKPWRMSELPMIPERFGIKLIDEQGIIKQFKTIERLFSEADEIINCG
                     DAGQEGEVIQRWVLQQTKCKKPVKRLWISSLTEEAIKDGFEHLRGSEDYDKLYAAGCA
                     RAIGDWLLGMNATRLYSLKYGNGKAVLSVGRVQTPTLALVVNRQLEIDNFVPQPYWEL
                     KTKYKDVVFQLKSGRFTNVEDAQKVLDTIKNSPFTIKSVETKNGKESSPRLFDLTSLQ
                     VECNRKFGYSADETLKIIQSLYEKKLTTYPRVDTTYLPTDVYPKIPEILKGITPYQKW
                     IEPLLTSKIRKSTQVFNDKKITDHHAIIPTGKFHNNFTPQEKWVYDLIARRFIAAFYP
                     DCSVANTVVEGITADVEFRVSGKQILEPGWRLLFENEKNQEDTETVIPEFIVGESGEH
                     TPDLAEKQTQPPKYFTEAGLLRAMETAGKMVDDEDIRDAMKENGIGRPSTRANIIETL
                     FKRRYIVRDKKRILATPTGIELISAIDNELLKSAELTGRWERKMREIEKGTYEMSTFM
                     VELRQMVADVVTQVKSARRRSITIVEQEIKKEEKPKQKITKKTNK"
     gene            complement(4997..5428)
                     /locus_tag="PK641_03705"
     CDS             complement(4997..5428)
                     /locus_tag="PK641_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55489.1"
                     /translation="MKILRYFLIFWTLFIGIGALWGTTMMFIDPSGEMWGMEELLYGM
                     QVLPYSEILFQDFIFPGIALFLVNGLFQIITSILLFSRNKYASIYGMCCGIILMLWIC
                     LQFYIWDPNPLSNAYFIFGLLEATNGFVLYRLERKKQHKKQ"
     gene            complement(5453..>6133)
                     /gene="scpB"
                     /locus_tag="PK641_03710"
     CDS             complement(5453..>6133)
                     /gene="scpB"
                     /locus_tag="PK641_03710"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF008506.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HPG55490.1"
                     /translation="VIKAQCNHGVWSAGHDIKELPINGEDPLSFDVPMERLLHLVQDL
                     PIPVIAYVEGSVWGGACDLCLSCDMIVAVDTATFAITPAKIGIPYNASGIMHFINQLG
                     INKAREMFFTGTPITAQDALNVGFLNQIAPIDKLPKLLEDKFLNPMRKNSILSISAIK
                     RQFRVLSRAAASISAENFESINAWREKVYRGEDYPEGIKAFLEKREPKFKGKASDLDH
                     TNVTRIVG"
BASE COUNT         1881 a         1177 c          924 g         2151 t
ORIGIN      
        1 catgtgcctt actacgcatt atttcagaac ctccatgcaa aacaataggg cctaaggttg
       61 tgtacaaaag tgtaacagca attttatatt catcttgata aacatctgtg cgcccatcaa
      121 aattcttacc aaattgatct gccaaagcaa aattatcgtg aatatccaaa tatgagattc
      181 cgctatttgg tgttttgtca tctttatatg aatttgtcaa acctttcatc acatttggtc
      241 tttcagaata cttaccacct gaccatcccc tatcgttttt cggatctttt aattcaaaaa
      301 caggtccttt atatgctgta cgcacctcat cttggaaata tgttattgga gaatcttctt
      361 tgtaccaatc ccaatcagga ttattttcgt aattaggatc attagatcca atccaaggtt
      421 caccgtatat tattttatct tttccgacaa tttcacgtaa ggctaacaga gtttgttgat
      481 ctatttgacc tgctaaatca attcgaaatc catctattcc gaattcatta ataaaataca
      541 aacattggtc aatcaaccaa cgttgtgtca ttggtctgtt ttctgtttta atttcattac
      601 cataaccacc tatgtgttcg aaattcttag ttctataata atattgttta tcgaatccgt
      661 taaaattaaa attccatgat tgcccgtcca tatcttctgc agtatggtta ggtacaatgt
      721 ctattatcac agcaatgttt ttatcatgaa atgcttgtac taaatcgcga aattgattac
      781 gttcagagcc aagttcagaa ccctttgtac gatagcgaga ttctacagcc attgcgaaag
      841 acgttctata tccccattga taattttctt cggatacacc ttgttctttc atatatggat
      901 cattttcgaa cgaagctttc caatcgtctg tcgggaaatg aagatactct tgtacaggca
      961 tcaaatgcac tgtatttatg cccaattcta ttagataatc aaaaccgatt tttttaccgt
     1021 tctcattttt aagtccggat gcaaccatac cttttaatgt accttttaag ctgtcgggca
     1081 aaggtaactt atctgtaaaa tcttgaacgt gaacctcata ggctattaca ttttccatag
     1141 taggtatgcc gttctttaat ggtttagcag gtactgtacg atgccatatc ctacattttc
     1201 caaaagtatc atcactaaca cgagcataag gatcatttac atgaaccgga cgtgtctcat
     1261 agaacaagtt ccccggatct ttagagccat gaactgtaaa atcatagtaa acaccgtgta
     1321 aatcttcatt gaagaaactt tcccatacac catctttatc ttgcttcatt tcttcgatgc
     1381 gatatgcctt atcatcatcc ttgttcttat atagatataa tttcaccttt tcggctctag
     1441 gagaaaaaac tcttatagtc gtttttccgt tatctatatt tgcgcccatt tctttacttg
     1501 agcacaattc tctaaaccaa ccatcataag agcatattac tttttgattt tgtgatggta
     1561 tttctaaata atatgctctg cgtttatcta atggagattc aggaacaatt agtgtcgttt
     1621 tagctccgtt tggtaaaact tttgctactt taattttatt cccttgtgca tctgaaataa
     1681 cgtaatctga aggtaaaaaa gacctatttg cacctatttc agcccaaatc aaattgtcgc
     1741 ttttaatttc tgcttttagt tctaatgatt catcttgttt catgaaaacc aaatcaccac
     1801 ccttttgatt aggagcccct gaaggaggtg acatccagtc tccattatca gttctgaatt
     1861 tgaattttgt atttggtttt actattgcaa aatcagtatt atctattgtc aatagccatt
     1921 gttgccctgt ttgtttcaaa ttccattcag gtgaattgat tgacgtatcc caatttcgaa
     1981 attcgcctgt aacaaaaact tttttaggag taacgccata taaagcttcg tcaaaaataa
     2041 aagtaagttg atcgccaaat acaccatatc cttgcaaggc ttgctctccg gtaaacgatt
     2101 gagcatgaag attcgccgta agcacaaaaa aaacggcaca aattaaactt aattttttca
     2161 taaattttgg tttagagtat tgatagaatg caaatataaa aaatatattt ctacttttgc
     2221 aaaacaaaat ttaattacaa actattaata catataatta tggaacaatt aaatcagtta
     2281 tgggagaatc tgattacatc aggaatcgat ttaggtaaga gtatcattgc agcatcggta
     2341 atttactttg ttggtagata catagttaag ctaattaaca aagtatttaa taagatgatg
     2401 gaaagacgca aactagaccc ggcagtaaaa tctttcttag gtagtttagt caatattact
     2461 ctaacaattt tattaataat cgcagttatt agcgcattag gaattgaaac aacatcattt
     2521 gcagcattat tagcatctgc cggcgttgca attggtatgg cattaagcgg aaatctgcag
     2581 aattttgcag gaggtttgat tatattactt ttcaaaccat ataaaatagg tgatgtagtc
     2641 gaaacacaag gaaaaacagg tttagttaaa gaaattcaaa tatttcatac tattattaca
     2701 acagctgaca acaaaacaat ttttattcca aacggaagca tcagtagtgg agtcttaacc
     2761 aattttagtg accaagactt acgccgtgtt gattggtcaa taggtattga atatggtgaa
     2821 gactacgaaa aagcaaaggc tgcaatagaa gaattattga atgctgataa gagaatactt
     2881 aaaactcctg aatattttat agctctcgaa caattagctg atagtaaagt aaatatcgca
     2941 attagggcat gggttaaaag tgcagattat tgggacgttt tctttgattt caacaagact
     3001 gtttattctg aatttaataa aaaaggcatc ggatttcctt tcccgcaaat tacagttcat
     3061 caatcaaaaa aatgattgta atatatacaa caaaaaagca gttcaataca gaactgcttt
     3121 tttaattaga tgcttattta ttagtctttt tagtaatttt ttgcttaggt ttttcttctt
     3181 ttttgatttc ttgttcaact atggttattg atcttcttcg ggctgattta acctgtgtta
     3241 caacatctgc aaccatttgt cgcaattcga ccataaaagt tgacatttcg tatgtcccct
     3301 tttctatttc acgcattttt ctttcccacc tacctgtaag ttcagctgat tttaataatt
     3361 cgttgtctat ggctgatatt aattctatgc ctgtaggtgt agctaaaatc cgttttttat
     3421 ctcttactat atacctgcgt ttaaaaaggg tttctataat attagcacga gttgatggac
     3481 gccctattcc attttctttc attgcatcac gaatgtcctc atcgtctacc attttacctg
     3541 ctgtttccat tgctcttagc aaaccggctt ctgtgaaata ttttggtggt tgagtttgtt
     3601 tttctgccaa atcgggtgta tgttcgccac tttcacctac tataaactca ggaataacag
     3661 tttcagtatc ttcttgattt ttttcattct caaataacaa tctccaaccc ggttcaagaa
     3721 tttgcttacc ggatactcta aattcgacat ctgcggtgat gccttcaaca acagtatttg
     3781 ctacactaca atcaggataa aaagctgcaa taaaacgtct tgcaattaaa tcgtagaccc
     3841 atttttcttg aggagtaaaa ttattatgaa atttgcctgt aggaataatg gcgtggtgat
     3901 cggttatttt tttatcgtta aatacctgag tcgatttcct tatttttgaa gttagtaagg
     3961 gttctatcca tttttgataa ggagttattc cttttaatat ttccggaatt ttaggatata
     4021 catcagtagg taaatatgtg gtatctacac gtggatatgt agttaatttt ttttcgtaca
     4081 atgattgaat aattttcaaa gtctcatcag ctgaatatcc aaacttccta ttacactcta
     4141 cttgtaatga cgtaaggtca aacaatctag gtgaactttc cttgccgttc ttggtttcta
     4201 ctgattttat tgtaaaagga ctgtttttaa tggtatccaa tactttttga gcatcttcaa
     4261 cattggtaaa tcttccggac tttaattgaa atacaacatc tttatactta gtcttaagtt
     4321 cccaatatgg ttgtggtaca aagttatcta tttctaactg tctattaaca actaatgcta
     4381 atgtcggcgt ttgtactcta ccaactgata atactgcttt accatttccg tatttaagtg
     4441 aatacaaacg agttgcattc attcccaata accaatcacc aatagctctc gcacatcctg
     4501 cagcatataa tttatcataa tcttcacttc cgcgtaaatg ctcaaagcca tctttgattg
     4561 cttcttctgt gagtgatgat atccataatc ttttaacagg tttcttgcat ttcgtttgct
     4621 gcaataccca tctttgaata acttcaccct cctgaccggc atcaccgcaa ttaataattt
     4681 catcagcctc cgagaataaa cgctctatgg ttttaaattg tttaataatt ccttgttcat
     4741 caatcaattt aataccaaaa cgctccggaa tcatcggcaa ctctgacata cgccatggtt
     4801 tccattcttc tttataatcg tgaggctctt ttaaagtaca taaatgacca aaagtccaag
     4861 taacttgata cccatttccc tcaatatagc cgtcacagcg tttggtagca cctaaaactt
     4921 gagctatatc ttttgccaca cttggctttt cggctataca aactttcatt aatatatcgt
     4981 tattcttgta agttttctat tgttttttgt gctgcttttt tcgctctaag cggtacaaaa
     5041 caaatccgtt agttgcttct aataaaccga atatgaaata cgcatttgac aatggattag
     5101 gatcccaaat ataaaattgc aaacaaatcc acaacatcaa aattatacca caacacatac
     5161 cgtaaataga agcatattta tttcttgaaa aaagcaatat agatgttatg atttggaaaa
     5221 gtccattcac caaaaacaag gctataccag ggaaaataaa gtcttgaaag agtatttctg
     5281 aatagggcaa tacttgcatt ccgtatagta attcttccat tccccacatt tcaccacttg
     5341 gatcaataaa catcattgtt gttccccata aggctccaat acctatgaaa agagtccaga
     5401 atattaaaaa ataacgtaat attttcatat tgaattaatt aaataaatat gtctaaccta
     5461 ctattcttgt tacatttgta tgatctaaat ctgatgcttt gcctttaaat ttaggctcac
     5521 gtttctctaa aaatgccttt attccttcgg gataatcttc ccctctataa actttttctc
     5581 gccatgcatt aatgctttca aaattttcgg cagatatact ggctgctgca cgacttaaaa
     5641 ccctaaattg tcttttaatt gcacttattg acaagattga atttttacgc attggattta
     5701 aaaatttatc ttctaacaat ttaggcaatt tgtcaattgg tgctatttga tttaaaaatc
     5761 ccacgtttaa agcatcttgt gccgttattg gtgttcctgt aaagaacatt tctcgagctt
     5821 tattgatacc taactgatta ataaaatgca ttatgcctga tgcattatat ggtataccaa
     5881 tttttgccgg tgtaatagca aatgttgcag tatcaactgc tacaatcata tcacacgaca
     5941 gacacaaatc gcaagcacct ccccatacag acccttctac atacgcaata accggaattg
     6001 gtaaatcttg taccaaatgt agcaatcgct ccatcgggac atcaaatgac aacggatctt
     6061 cgccattgat aggtaattcc ttgatgtcat gtcctgcact ccagactcca tgattacatt
     6121 gagccttgat tac
//
LOCUS       DAOPID010000076         6123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_16808_length_6123_cov_4.326796, whole genome shotgun sequence.
ACCESSION   DAOPID010000076 DAOPID010000000
VERSION     DAOPID010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 6123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6123
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16808_length_6123_cov_4.326796"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..1826)
                     /locus_tag="PK641_03715"
     CDS             complement(213..1826)
                     /locus_tag="PK641_03715"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047334 - diphosphate-fructose-6-phosphate
                     1-phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HPG55491.1"
                     /translation="MTKSALQIARAAYQPKLPKALKGAVVIKEGKSTESVADQAEIKK
                     LFPNTYGMPVVTFEPATAAEKLPAMNVGVILSGGQAPGGHNVISGLFDGLKTMNKDCK
                     LYGFLGGPGGLVDHKYVELTSNIIDEYRNTGGFDIIGSGRTKLEETSQFDSGIEICNK
                     LGIKALVIIGGDDSNTNACVLAEYYLQKKCGIQVIGCPKTIDGDLKNDMIETSFGFDT
                     ACKVYSELIGNIMRDANSAKKYWHFIKLMGRSASHIALECGLQTQPNICIVSEEVEAK
                     KMTLSQIVSNIADTVAVRAAAGNNFGVVLIPEGLIEFIPEMKILISELNDLLAHESDV
                     KKAVSGLSPASAAVYASLPEAIAKQLTLDRDPHGNVQVSLIETEKLLIEMVKTRLSAM
                     KAEGKYVGKFSGLGHFFGYEGRCAAPSNFDADYCYSLGFNAANLIANGKTGYMSLIRN
                     TTASAEKWIAGGVPVTMMMNMERRHGEMKPVIRKALVELEGAPFKKLVANRAEWATVT
                     SYLYPGPIQYFGPSEVCDQPTKTLQLERA"
     gene            complement(1959..3284)
                     /locus_tag="PK641_03720"
     CDS             complement(1959..3284)
                     /locus_tag="PK641_03720"
                     /inference="COORDINATES: protein motif:HMM:NF028184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH36 C-terminal domain-containing protein"
                     /protein_id="HPG55492.1"
                     /translation="MNKYDLASLGVGLVKMDYWWTVPTTGSDPNSGFDSYLETQWLPN
                     AQKWPNGMLFGIMTKDKYPTVQNSLYMCDTYQGCDISTKAGRDAQIAALSERITNWKI
                     DYWRSDFDLEIPNNYMSHEGLLLIIDSLSNKHSNFRYEHCSAGGSLKDFSSLRCMAFM
                     TMEDSGGPLNHRMAFYSNSYMINPVQLKFDVGFDWSSPSDATNISANPTQWSYYVLRS
                     SMMGAMMACAVGRDMSSIEFEAAQNCWRLYNNKQRNILRGADVYHILPMPDGKHWDGI
                     QFYNPEIAKGSVFLFSHLSNGGNDGTQKRIYLSGLDKNASYQLSFEDRTGLNCVRSGS
                     DLMQNGILVTGMSKAFDSEIIWIEQLSSSVDATQSEGMCLYPNPVAAGQNVKVLGTDL
                     ESCTIKIFDMSGRLIETGITLNSLGFSAPQMQGCYLININGTTQKLIVR"
     gene            complement(3281..3949)
                     /locus_tag="PK641_03725"
     CDS             complement(3281..3949)
                     /locus_tag="PK641_03725"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HPG55493.1"
                     /translation="MRTGLFCFIILLTNICSVCALQAQNLIANPSFEQMNESWSVWPN
                     ANISTSKAHEGTHSLQSTMRGLNGATGASNASISVSKNTYYEYSAWIYRNDNSAWAYI
                     DMNDAPGELQLRASKFGQWEKVSGLWYSGETTSVSLRCIVERDWQNNSPSYGGVTGDV
                     WFDEIYFAKLGREPYSESTSELTPNALYWTIENDDIQAVIAEDESKVYICNLVNLKMI
                     TGII"
     gene            complement(3980..4927)
                     /gene="cysK"
                     /locus_tag="PK641_03730"
     CDS             complement(3980..4927)
                     /gene="cysK"
                     /locus_tag="PK641_03730"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HPG55494.1"
                     /translation="MSKIAKKLTELVGNTPLLELERYASTKNLQASLIAKLEYFNPAG
                     SVKDRIALSMIEEAEKAGALKPGATIIEPTSGNTGVGLAFVSRVKGYHLILTMPDSMS
                     LERRNLLKAYGAELVLTPGAVGMKGAIEKAEELRNSIEGSIILQQFTNPANPLTHYKT
                     TAEEIWRDTEGKVDIFVAGVGTGGTVSGVGRRLKELNPNIKIVAVEPETSQVLAGKPA
                     GPHKIQGIGANFVPANFDRSVVDEILPVQSDDAIRTSRELSATEGLLVGISSGASAYG
                     ATILAQRIENKSKKIVALLPDTGERYLSTVLYDFENYPL"
     gene            complement(5005..>6123)
                     /locus_tag="PK641_03735"
     CDS             complement(5005..>6123)
                     /locus_tag="PK641_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HPG55495.1"
                     /translation="GRLTNPTQSVFEERIATLEGGVAGLAVASGAAAISYAIQSITRS
                     GDHVVSAKTIYGGSYNLLAHTLSNYGISVTFVDPSDINNFRRAIQANTKLIFIETLGN
                     PNSNVIDIDAVADIAHSNKIPLIVDNTFATPYLLRPIEHGADIVVHSATKFIGGHGTS
                     LGGVIVDGGKFDWKGSGKFPQLSEPDPSYHGLVFADAVGPAAYVVRIRAVVLRDTGAA
                     ISPFNSFLLLQGLETLSLRVERHVENSLKVVDFLKKHNKVEIVNHPYLSSHPDHKLYK
                     RYFPKGAGSIFTFRIKGDAKTAQQWIDNLQIFSLLANVADVKSLVIHPASTTHSQLSA
                     SELLEQEIYPNTIRLSIGTEHIDDIIYDLQQAFDKIKV"
BASE COUNT         1900 a         1261 c          947 g         2015 t
ORIGIN      
        1 taaagcatgc atcatatcat tacagagtga gttgctaaga caattcaatt tttcgggatt
       61 ttgcatttct ataaagcaaa tcctatcgac ataagaaatt ccaatcatat cgtgtttttt
      121 taataatgaa tctgtgttta taaaaaaagt cgggcggcat aatcatattt gatttcacca
      181 cccgactcta taaatattta aagcacaata ctttatgctc tttctaattg taatgtctta
      241 gtcggttggt cgcaaacttc agaaggtccg aaatattgta taggaccggg atataaataa
      301 cttgttactg tagcccactc agcacgattt gctactaatt ttttaaatgg agctccttcc
      361 aattctacta aagctttgcg aataacaggt ttcatttcgc catgacgacg ttccatgttc
      421 atcatcattg taacaggaac accacctgca atccattttt cggcagaagc tgttgtattc
      481 ctgattaatg acatgtatcc ggttttaccg ttcgcgatta agtttgctgc gttaaatcct
      541 aatgaatagc aataatctgc atcaaaattt gaaggagcag cacaacgacc ttcatatcca
      601 aagaaatgac ctaaaccgga gaatttacca acatattttc cctctgcttt cattgctgat
      661 aaacgtgttt taaccatttc tatcaacaat ttctctgttt cgattagtga tacttgtaca
      721 tttccgtgag ggtcacgatc taaagtcaat tgttttgcaa ttgcttcagg caatgatgca
      781 tatacagctg cacttgcagg acttaatccg gaaacggctt tcttaacatc tgattcgtga
      841 gctaatagat cattaagttc tgatattaaa attttcattt caggaataaa ttcgatcaag
      901 ccttccggga ttagaacaac tccaaaatta tttccggctg ctgcacgtac agctactgta
      961 tctgcaatat ttgaaacgat ttgagacaaa gtcatttttt tagcttcaac ttcttctgat
     1021 acaatacaaa tattaggttg tgtttgtaat ccgcattcaa gagctatatg tgatgctgaa
     1081 cgacccatca atttgataaa gtgccagtat ttctttgcag aatttgcatc acgcatgata
     1141 ttaccgataa gttctgaata aactttacat gcagtatcaa aaccgaaaga ggtttcaatc
     1201 atgtcgtttt tcaaatctcc atctatggtt ttagggcaac ctataacttg gatgccacat
     1261 ttcttctgaa gataatactc agcaagtacg catgcatttg tgttagagtc atctccacca
     1321 ataattacta atgctttgat tcctaattta ttgcagattt cgataccgct atcaaattgt
     1381 gaagtttctt ccaattttgt acgtcctgaa cctattatat caaaacctcc tgtgttacga
     1441 tattcatcaa taatatttga tgttaattca acatatttgt gatcgacaag tccgcccggg
     1501 ccacctaaaa atccatacaa tttgcaatct ttgttcatag ttttcaaacc atcgaacaag
     1561 cctgatatta cattgtgtcc accgggagct tggccacctg aaagaataac acctacgttc
     1621 attgcaggta atttttctgc tgctgtagca ggttcaaatg ttacaacagg cattccgtat
     1681 gtattaggaa ataatttttt aatttctgct tgatcagcaa ctgattctgt agatttacct
     1741 tctttaataa ctacagctcc tttcaaagct ttgggtaatt ttggctgata agcagctctt
     1801 gcaatctgta atgcgctctt tgtcatatcg ttataattaa taattaattc aaaacaaaca
     1861 gacgcaaatt tataaaaata tctttaaata acgctctaaa tatattaata aattattaaa
     1921 cacaatttca aaaagctgtc aaaaccgcag ctatacaatt atctgacaat taatttttgt
     1981 gtggtaccat ttatattaat taaataacag ccttgcatct gaggtgcact aaaacctaaa
     2041 gaatttaatg ttatgcctgt ttctattaat ctacctgaca tatcaaatat ttttatagta
     2101 caactttcta aatcagtgcc tagaactttt acattttgac ctgctgcaac aggattagga
     2161 tacaaacaca taccttcaga ctgtgttgca tcaactgatg atgataattg ttcaatccaa
     2221 ataatttcag agtcgaaagc ttttgacatt ccggtaacaa gtattccatt ttgcatcaaa
     2281 tcggatccac tgcgaacgca attaagtccg gttctatctt caaatgataa ctgatacgag
     2341 gcatttttat ccaatccgct tagatatatt ctcttttgcg ttccgtcgtt gcctccgttt
     2401 gataaatggc taaataagaa cacagaaccc ttagctatct ccggattata aaattggatt
     2461 ccatcccaat gcttaccatc aggcatcggt aatatgtgat aaacatcagc tcctctcaaa
     2521 atatttctct gcttgttgtt atacaatctc caacaatttt gagcagcttc aaattctatg
     2581 ctactcatat ctcttccaac tgcacaagcc atcattgctc ccatcataga tgaacgtaat
     2641 acatagtaag accattgagt tggatttgct gatatatttg ttgcatcact cggagaactc
     2701 caatcaaaac ccacatcaaa ttttaattgc acaggattaa tcatgtacga atttgaataa
     2761 aatgccattc gatgatttag cggacctcct gaatcttcca ttgtcataaa cgccatacag
     2821 cgcaaactgg aaaaatcttt taacgatcct cccgcactac aatgttcata tctaaaattg
     2881 gaatgtttgt ttgatagaga atcaattatc aacaataagc cttcgtgact catataatta
     2941 ttgggaattt ccaaatcgaa atcactacgc caataatcaa ttttccaatt tgttatacgt
     3001 tcgctcaatg cggcaatttg agcatcacga cctgcttttg ttgatatatc gcagccttgg
     3061 taagtatcgc acatataaag tgaattctgc acagtgggat atttatcctt tgtcataatt
     3121 ccaaaaagca ttccgttagg ccatttctga gcattaggca accactgagt ttctaaataa
     3181 gaatcgaagc ccgaattagg gtcagaacct gttgttggaa cagtccacca ataatccatt
     3241 tttactaaac ctacgcctaa agatgctaaa tcgtacttat tcaaataata cctgtaatca
     3301 ttttcagatt aactaaatta catatataaa cttttgattc atcttctgca attactgcct
     3361 gtatatcatc attttcaatt gtccaataaa gcgcatttgg agtcaattct gaagtagatt
     3421 cgctgtatgg ttcacgaccc aacttagcaa aatatatttc atcaaaccac acatcacctg
     3481 tcacacctcc atacgaaggg ctattatttt gccaatctcg ttcaactata caacgtaagc
     3541 ttacagaagt agtttcgccg ctataccaca aaccggatac cttttcccat tgcccgaatt
     3601 ttgatgctct gagctgtaac tcacccggag catcattcat atcaatatat gcccatgcac
     3661 tattatcatt acgatatatc caagcactat attcataata agtatttttg ctaacagata
     3721 tacttgcgtt tgatgcaccg gttgctccgt ttaagcctcg cattgtagac tgcaaagaat
     3781 gcgtaccttc gtgtgcttta cttgttgata tattagcatt tggccaaaca gaccacgact
     3841 cattcatttg ttcaaaagat ggattcgcaa tcaaattttg agcctgtaaa gcacatacag
     3901 aacatatatt agtaagcaaa ataataaaac agaacaatcc ggttctcata gtataggttt
     3961 gttttcggtt tattccttat tacaaaggat aattctcaaa atcgtacaga acagtagata
     4021 aataacgttc gcctgtatcc ggtaacaaag ctactatttt ttttgattta ttttcaatgc
     4081 gttgagctaa tattgtagct ccataagctg atgcaccaga cgaaattcct accaaaagcc
     4141 cttctgtagc ggataattcg cgactggttc ttattgcatc atcactttgt acaggcaaaa
     4201 tttcatcaac aacagatctg tcgaagtttg caggcacaaa atttgcgcct atcccctgaa
     4261 ttttatgagg tccggcaggt ttacctgcca atacttggct agtttccggt tcaactgcaa
     4321 ctatcttaat attaggattt aactctttca aacggcgtcc tacaccactt accgtacctc
     4381 ctgtgcccac gcctgctaca aagatatcta ccttaccttc agtatcacgc caaatttctt
     4441 cggcagtcgt cttgtaatga gttaatggat ttgccggatt cgtaaactgt tgtaaaatta
     4501 tcgaaccttc aatgctatta cgcaattctt cagccttttc tatagcgcct ttcattccta
     4561 ctgcaccggg agttaagacc aattctgcgc cataagcctt taatagattc ctacgttcca
     4621 aactcattga atcaggcata gtcaatatca gatgatagcc ttttactcta ctgacaaaag
     4681 ccaatcctac tcctgtattc ccactagttg gttctattat tgtagcaccg ggttttaatg
     4741 cacctgcttt ttctgcttct tctatcatag aaagagctat gcgatctttt acactacctg
     4801 cggggttgaa atactctaat ttagcgatta aacttgcttg caaatttttt gttgaagcat
     4861 atctttcaag ttcgagtaat ggtgtattac ctactaactc ggttaatttt tttgcaatct
     4921 tagacatgat gtttatattt taattattgt ttttattatg aaaagtttaa caaagatact
     4981 aataagcaga tatgattaat ctccttatac tttaatttta tcaaaagctt gttgtaaatc
     5041 atatattata tcatcaatat gttcggtacc tattgataag cgaattgtgt ttggataaat
     5101 ctcttgctct aataattctg atgccgaaag ttgactatga gtagtacttg caggatgtat
     5161 taccaaactc ttaacatccg ccacatttgc caaaagagaa aatatttgta aattatcaat
     5221 ccattgttga gctgtttttg catcaccctt aattctaaag gtaaatatac ttcctgctcc
     5281 tttagggaaa tagcgtttat ataatttatg atcaggatgt gaacttaaat atggatgatt
     5341 aactatttca actttattgt gtttcttcag gaaatcaaca actttaagcg aattttcgac
     5401 atgacgttca acccttaaag ataaagtctc taaaccctga agcaataaaa atgaattaaa
     5461 cggagaaatt gctgctcctg tgtcacgtaa aacgactgct cgaatacgaa caacataagc
     5521 tgcaggtcca actgcatcag caaaaaccaa tccgtgataa gaaggatcag gttcgcttaa
     5581 ttgaggaaat ttaccactgc ctttccaatc aaatttacct ccgtcaacaa ttacacctcc
     5641 aagcgaggta ccatgtccac cgataaattt tgttgcagaa tgaaccacaa tatcagcacc
     5701 atgttctata gggcgtagca aataaggagt agcaaatgta ttatctacta ttaatggaat
     5761 tttatttgaa tgagcaatat ctgcaactgc atcaatatct ataacattac tgttaggatt
     5821 acctaatgtc tcaataaaaa ttaattttgt atttgcttgt attgcccttc taaaattatt
     5881 gatatcagaa ggatcaacga aagttacaga aattccataa ttggacaatg tatgagccaa
     5941 taaattataa ctacctccat atatagtttt tgcagaaact acatggtcgc cgcttctggt
     6001 aatactctgt atagcgtagc taatagcggc tgcaccacta gctacagcaa gccctgcaac
     6061 accaccttct agagttgcaa tcctctcctc aaatacgctt tgcgtaggat ttgtgagacg
     6121 acc
//
LOCUS       DAOPID010000077         6113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_16845_length_6113_cov_4.330802, whole genome shotgun sequence.
ACCESSION   DAOPID010000077 DAOPID010000000
VERSION     DAOPID010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 6113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6113
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16845_length_6113_cov_4.330802"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="PK641_03740"
     CDS             complement(<1..281)
                     /locus_tag="PK641_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HPG55496.1"
                     /translation="MNNQRYMMRGVSASKEDVHNAIKNIDKGIYPRAFCKIIPDILGG
                     DAEYCNIMHADGAGTKSSLAYLYWKETGDLSVWKGIAQDALIMNIDDLL"
     gene            complement(284..1141)
                     /locus_tag="PK641_03745"
     CDS             complement(284..1141)
                     /locus_tag="PK641_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPG55497.1"
                     /translation="MKKNNWKDKDSKFAGIIGTIAVHALLLLAILFCGIKIYLPQEEE
                     GLTVNYGTSDTGEGLFEPAPQSAIEDQLEDIPDATNPPLINNSSKSELQTQNIEESLE
                     LKEAKKKIEEAKKQELERQRLLAEQKRQEEEKRKIEEAKRAKAASAAKSAFGKGGKGT
                     DTNATGEGITGGAGNQGNPFGDVNSKNRVGGGTGSGHSYNLNGRSLSGKIPEPKYDKN
                     EEGIIVVSIEVDPSGKVISAKSGALGTTIGDASMRKEAEIAAMRATFNGISGSTVQSG
                     TITYRYKLN"
     gene            complement(1138..1554)
                     /locus_tag="PK641_03750"
     CDS             complement(1138..1554)
                     /locus_tag="PK641_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPG55498.1"
                     /translation="MALKKRNKINASFSMSSMTDLVFLLLIFFMITSTVVTPSAIKLL
                     LPQSSSQAAAKPITRIYIDENINFYVAEGKQQERQVTFEEIEPFLQSVVAAEPEMYVA
                     LYADESVPYREIVRVLNIANKNKFKLVIATRPVKES"
     gene            complement(1561..2283)
                     /locus_tag="PK641_03755"
     CDS             complement(1561..2283)
                     /locus_tag="PK641_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPG55499.1"
                     /translation="MNFLNILLQIPLAAESAVDSTMQTVVTTTPEVIEMNYFEMAKKG
                     GPIIMIPLLILSIIAVYIFIERLVVIHKASKRDKSFMDRIKDYITEGKVDSATKLCQQ
                     VNTPASRMIEKGISRIGKPMNDVLIAIENVGNIETNKLERGFSMLATIAGGAPMLGFL
                     GTVIGMVQAFFDMANAGNNIDITVLSNGIYTAMITTVAGLIVGITAYFAYNYLVSKLD
                     SVVQKMELSTMEFMDLLNEPIK"
     gene            complement(2287..3003)
                     /locus_tag="PK641_03760"
     CDS             complement(2287..3003)
                     /locus_tag="PK641_03760"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HPG55500.1"
                     /translation="MTKLSVNINKVATIRNARGGNTPNVEQVAIDCEKFGAQGITVHP
                     RPDERHIRYSDVRELKKLVTTEFNIEGYPQQSFIDLVLEVVPTQVTLVPDPPEALTSN
                     AGWDTVKHLQFLEETVKIFHSKGIRVSIFTDTDLKNIEYAAKTGTDRIELYTEPYAEQ
                     FLVDKFQAVKPFAEAAAFAHKCKLGVNAGHDLSLVNLKYFAQNVPFLDEVSIGHALIS
                     DALYFGLESTIQKYRQQLTF"
     gene            3114..3995
                     /locus_tag="PK641_03765"
     CDS             3114..3995
                     /locus_tag="PK641_03765"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HPG55501.1"
                     /translation="MRVAVFGSIHKKGFSNACDMLLQALERHEADVFLFDEVYKHLLS
                     VKAYLPSNYKVLQDGDDIQVDLALSLGGDGTFLKTAQRIGTRRIPILGIKLGNLGFLV
                     DVQSNEISAAIDDVFENGIQIEERSMLQLKTDKQRFTNGETIALNEIAVLKLDISSMI
                     KVHTIVNREYLCTYRADGLLVSTPTGSTAYALSVGASILVPQNKSFILSPVAPHSLNL
                     RPVVIPDDWKIELEVESRNDSFLISLDGRSQVFDKHTKLIITKADYSILLAKRKGHTY
                     FKTLREKLMWGMDARSN"
     gene            4002..4589
                     /gene="ruvC"
                     /locus_tag="PK641_03770"
     CDS             4002..4589
                     /gene="ruvC"
                     /locus_tag="PK641_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPG55502.1"
                     /translation="MNTEIQTAKSGLPYVILGIDPGTTVMGYGLIRVQGNKVEMLAMG
                     VLELKKLADHYEKLKRIFARVLSLIDEFDPQVLSIESPFFGKNVQSMLKLGRAQGVAM
                     AAALYRDITVQEYTPLKIKMSITGRGSASKEQVADMLKRYLKITPEQMLPQLDATDAL
                     AAAYCHYLQIGNPSIKPEYKSWKDFISQNDKRVKK"
     gene            complement(4590..5315)
                     /locus_tag="PK641_03775"
     CDS             complement(4590..5315)
                     /locus_tag="PK641_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPG55503.1"
                     /translation="MGRAFEYRKATKMKRWGNMARVFTKLGKQITIAVRESGSDPDGN
                     PRLRVLIQQAKKENMPKENVERAIKKAFAKDENDYKELNYEGYGPHGIAIFVETATDN
                     NTRTVANIRSYFTRYGGSLGTTGCLQFLFDHKCVFNVCPKDGVSMDDMELELIDFGVE
                     EIDKDDDGNLIIYGDFQSYSAIQKYLEDNGFEINSAEFERIPNDYKKVTEEQRASIQK
                     LLDKIEDDEDVQNVYHNMAAEEE"
     gene            complement(5341..>6113)
                     /locus_tag="PK641_03780"
     CDS             complement(5341..>6113)
                     /locus_tag="PK641_03780"
                     /inference="COORDINATES: protein motif:HMM:NF036799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPG55504.1"
                     /translation="LLFGGLESIAYNRNRRRTNLKFYEFGTCYHYHPENAGSEQALAA
                     YSEEPHLGIWLSGNSYEAIWTDSERKLTFFDLKAHVINVLARLGIASNSYLTTKTEID
                     IFSQAIKFETRGGKTLVTLGIISPKLLKAFDIDTEVYYADMEWNQILKQVKNNKTSYK
                     DLPKFPEVKRDLALLLDKNIYFEQIENIAFETERKLLKKVVLFDVYEGKNLEEGKKSY
                     AVNFTIQDSEKTLEDKQIDKIMQNLIKAFTERLGAVIR"
BASE COUNT         1801 a         1124 c         1051 g         2137 t
ORIGIN      
        1 caaagtaaat catcaatatt cataattaaa gcatcctgtg caataccttt ccatactgat
       61 aaatctcctg tttctttcca ataaagatag gctaatgatg atttagtacc cgctccatct
      121 gcatgcatga tgttgcagta ttcagcatct ccaccgagta tatcaggtat aattttacag
      181 aaggctctgg gatagatacc tttgtcaatg ttttttatgg cgttgtgaac gtcctctttt
      241 gatgcagaca ccccgcgcat catataacgt tgattattca tgtctaattt aatttgtacc
      301 tataagttat agttcctgat tgtacggttg aaccggatat cccattgaat gttgcacgca
      361 ttgcagctat ttctgcttct tttcgcatag atgcatcacc aattgttgta cctagtgcac
      421 cggattttgc acttattact ttgccggaag gatcaacttc aattgaaaca acgattattc
      481 cttcttcatt tttgtcgtat ttaggttccg gaatttttcc tgacagacta cgaccattaa
      541 gattgtaact atgtccacta ccggtacctc cgccaacacg atttttagaa tttacgtctc
      601 caaaaggatt accttgattg ccggcaccac ccgttatacc ttcgccggtt gcgttagtat
      661 cagtaccttt acctcctttg ccgaaagcac tttttgctgc actagcagct ttagcacgct
      721 ttgcttcctc tattttgcgt ttctcttctt cttggcgttt ttgctcagcc aataagcgtt
      781 gacgttctaa ttcttgcttt ttagcttctt caattttctt tttagcttct tttagctcta
      841 gtgattcctc tatattttga gtttgtaatt cgcttttaga agaattattg attaatggag
      901 gatttgttgc atcaggaata tcttcaagtt ggtcttcgat tgcactttgt ggtgcaggct
      961 caaataaacc ttctcctgtg tcgcttgttc cataatttac ggtcagacct tcctcttctt
     1021 gcggcaaata aatttttatg ccgcaaaata gtatagccaa cagcaaaaga gcatgaacgg
     1081 ctattgtccc aataataccg gcaaatttac tgtctttgtc tttccaattg ttttttttca
     1141 tgattcctta acaggtctag ttgcgataac aagtttaaat ttgtttttat ttgctatgtt
     1201 aagtacacga acaatctcgc gataaggaac agactcatct gcatatagag caacatacat
     1261 ttcaggttca gcagcaacta cagattgtag aaatggttca atttcttcaa atgttacttg
     1321 gcgttcttgt tgtttgcctt ctgctacgta aaaatttata ttctcgtcaa tatatattct
     1381 agtaataggt tttgccgctg cttgactgct actttgcgga agtagcaatt ttatagcact
     1441 tggagtaaca actgttgatg ttatcataaa aaatatcagc aacaaaaaca ccaagtccgt
     1501 catagaggac atgctaaaag acgcattaat cttgtttctt ttttttagag ccatggctga
     1561 ctatttaatt ggttcattca ataaatccat aaattccatg gtgcttaatt ccatcttttg
     1621 tactacagaa tctaattttg atactagata gttatatgca aaatatgcag taatacccac
     1681 aattaatccg gctacagttg taatcatggc agtataaata ccatttgata aaactgtaat
     1741 atcaatgtta tttcctgcat tagccatatc aaagaatgct tgtaccatac ctattacagt
     1801 tcctaagaat ccaagcatgg gagcaccacc ggctatagta gctaacatcg aaaatccgcg
     1861 ttctaatttg ttagtttcaa tattaccaac attttcgatt gctataagta catcattcat
     1921 tggtttacct atacgtgaaa ttcctttttc aatcattctt gatgcaggag tattaacttg
     1981 ttggcataat tttgtagcag aatcgacttt gccttctgtt atatagtctt tgatgcgatc
     2041 cataaatgat ttatctcttt tagatgcctt gtgaattaca actagacgtt caataaaaat
     2101 ataaacagct ataatagaaa gaattagcaa aggtatcata attataggac cacctttttt
     2161 tgccatttca aagtaattca tttcaatcac ttccggcgtc gtagttacaa ctgtttgcat
     2221 agtgctgtca actgcagact ctgctgccaa tggaatttgc aaaaggatat tcaaaaagtt
     2281 cataatttag aaggttaatt gttgtctgta tttttgtatt gttgattcta atccgaaata
     2341 taaggcatca gatattaatg catggcctat tgatacctcg tcaaggaaag gaacattttg
     2401 ggcgaaatac ttcagattta ccaaactaag atcatgcccg gcatttactc caagcttgca
     2461 tttatgagca aaagcagctg cttctgcaaa aggttttacc gcttgaaatt tgtcgactaa
     2521 aaattgttca gcgtaaggtt ctgtgtataa ttcaatgcga tcggttcctg tttttgcagc
     2581 atattctatg ttctttagat ctgtgtctgt aaaaattgaa accctgattc ctttactatg
     2641 aaatattttg accgtttctt ccaaaaattg aaggtgttta acagtgtccc atccggcatt
     2701 tgaagtaagc gcttcaggtg gatccggaac caaagtaacc tgagtaggga caacttccaa
     2761 taccaaatca ataaatgatt gttgaggata tccttcaata ttgaattctg tagtaactaa
     2821 tttctttaat tcgcgcacat ccgagtagcg tatatgacgt tcatcaggtc gtggatgaac
     2881 cgttatgcct tgtgcgccaa acttctcaca atcaattgct acttgctcaa cattaggagt
     2941 attaccgcct cgtgcattac gtatcgttgc aaccttattt atatttacgc taagttttgt
     3001 cattataatt cagtaaaaat tacttacttt gtaagtccgt ttataaaaga cgtacaaagt
     3061 taattattta ttattgtttc accttgtttc aatgtataaa gttttccaca attatgagag
     3121 tagcagtatt tggcagtatt cataaaaaag gattttcaaa tgcttgtgat atgttgttgc
     3181 aagctttaga acgtcatgag gcagatgtct ttttatttga tgaggtatac aagcatttgc
     3241 ttagcgtaaa agcttatttg ccttcaaact ataaggtttt acaagacggt gatgacatac
     3301 aagtagattt agccttgagt ctaggtggag acggaacttt cttaaaaaca gcacaaagga
     3361 taggaacaag acgaatacct atacttggaa ttaaattagg aaatttgggt ttcttagtag
     3421 atgttcaaag taatgaaata tctgcagcta ttgatgatgt ttttgaaaac ggcatacaaa
     3481 tagaggaacg cagtatgctt caacttaaaa ccgataaaca gcgatttaca aacggcgaaa
     3541 ccattgcttt gaatgaaata gcagtactta aattagatat atcatcgatg ataaaggtgc
     3601 atacaattgt taatcgtgag tatttatgta cctatcgtgc agatggtcta ttagtatcta
     3661 ctcctacagg ttcaactgcc tatgctttaa gtgttggagc ttctatattg gttcctcaaa
     3721 ataaaagttt tatcttatct cctgtagctc cgcatagttt aaatctaagg cctgtagtta
     3781 tacctgacga ttggaaaatt gaattggaag tagagagtcg aaatgatagc tttttgattt
     3841 ctttggatgg tcgttctcag gtttttgata aacatacaaa actgattata acaaaggctg
     3901 attacagcat tcttttagca aagagaaaag gtcatacata ttttaaaacc ttacgtgaaa
     3961 aattgatgtg gggtatggat gcccgttcta attaaaacaa aatgaacacc gaaattcaga
     4021 ctgcaaaatc gggtttgcca tatgtgatac taggtataga tccgggtact acagtaatgg
     4081 gatacggact tatacgggta caaggtaata aagttgaaat gcttgcaatg ggtgtacttg
     4141 agttgaaaaa gctagcagac cattacgaaa agctaaaaag gatatttgca agggtacttt
     4201 ctttaattga cgaatttgat cctcaagtat tgtcaataga atctccgttt tttggtaaaa
     4261 atgtacaatc aatgctaaag ttgggtaggg cacaaggagt tgccatggct gcagcacttt
     4321 atagagatat cactgtacaa gaatacactc cattgaaaat taaaatgtcg ataacaggaa
     4381 gaggctctgc atcaaaagag caggttgccg atatgttgaa acgttatctt aaaataacac
     4441 ctgagcagat gcttccgcaa ttggatgcta cagatgcact tgcagctgca tattgtcatt
     4501 atttacaaat aggaaatcct tccattaaac ctgaatacaa gtcttggaag gattttattt
     4561 ctcagaatga taagcgtgta aagaagtaat tattcttctt ctgctgccat attgtgataa
     4621 acattctgaa cgtcttcgtc atcttcaatt ttgtctaata acttttgaat agaagcacgt
     4681 tgttcttctg ttactttttt atagtcatta ggaatacgtt caaattcagc actattaatt
     4741 tcaaatccgt tgtcttctaa atatttttgt attgctgagt atgattggaa atctccgtat
     4801 ataattaaat ttccatcatc atctttgtct atttcttcaa caccgaaatc aatcaattcc
     4861 aattccatgt cgtccataga tactccatct ttaggacaca cattgaatac acatttgtga
     4921 tcgaacagaa attgtaaaca acctgttgtt ccgagtgagc ctccgtatct tgtaaaataa
     4981 ctacgtatgt tagctacagt acgagtgttg ttatcagttg ctgtttctac aaatattgca
     5041 attccgtgag gaccatatcc ttcgtaatta agttctttgt aatcattctc atccttagcg
     5101 aatgcctttt taatagcgcg ttcaacattc tctttaggca tgttttcttt ctttgcctgt
     5161 tgaatcaaca cgcgtaaacg agggttacca tcaggatcgc ttccgctttc gcgtactgca
     5221 attgtaattt gttttcctaa cttagtaaaa acgcgggcca tatttcccca acgtttcatt
     5281 ttggttgctt tcctatattc aaatgctctt cccataacga tatttattat gataatttta
     5341 ttatctgatt actgctccaa gtctctctgt aaatgctttg attaaatttt gcattatttt
     5401 gtctatctgc ttgtcttcca atgtcttttc agaatcctga atagtgaaat tgactgcgta
     5461 agatttttta ccttcttcaa gatttttccc ttcataaaca tcgaacaaaa ctactttttt
     5521 caataattta cgttctgttt caaatgcaat attttcgatt tgttcgaaat atatattttt
     5581 atccagcaat aaagccaagt cgcgtttaac ctcagggaat tttgggagat ctttatagct
     5641 cgttttattg ttctttactt gtttaagaat ttggttccat tccatatctg catagtaaac
     5701 ttcagtatca atgtcgaatg cttttaataa tttcggagaa ataatgccaa gtgttactaa
     5761 tgttttgcct ccacgcgttt caaatttaat tgcttgtgag aaaatatcga tctcggtttt
     5821 tgtggttaaa tagctgttag atgctatgcc taagcgagcc aagacattaa ttacatgagc
     5881 ttttaaatca aagaatgtca atttgcgttc gctatcagtc caaatagctt cgtaactatt
     5941 tccggatagc caaatgccta aatgaggctc ttcagaatat gctgctaatg cttgttcact
     6001 acctgcgttt tcaggatgat agtgatagca agttccaaat tcatagaatt ttaaattagt
     6061 acgacgacga tttctgttat atgctatact ttctaaacca ccgaacaaca atg
//
LOCUS       DAOPID010000078         6037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_17167_length_6037_cov_4.341525, whole genome shotgun sequence.
ACCESSION   DAOPID010000078 DAOPID010000000
VERSION     DAOPID010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 6037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6037
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17167_length_6037_cov_4.341525"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3625)
                     /gene="rpoB"
                     /locus_tag="PK641_03785"
     CDS             complement(<1..3625)
                     /gene="rpoB"
                     /locus_tag="PK641_03785"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPG55505.1"
                     /translation="MSNQRINFSSIKNPLEYPDFLEVQLKSFQDFFQLDTPPEKRKNE
                     GLYKVFAENFPIADTRNNFILEFLDYYVDPPRYSIQECIDRGLTYSVPLKAKLKLYCT
                     DPDHEDFETVIQDVYLGPIPYMTEKGTFVINGAERVIVSQLHRSPGVFFGKSMHANGT
                     KLYSARIIPFRGSWIEFTTDINNVMYAYIDRKKKLPVTTLLRAIGFESDKDILEIFNL
                     AEEVKVSKASLKKSVGRTLAARVLKTWVEDFVDEDTGEVVSIDRNEVIIDRETVLSTE
                     HIDTILEANVSTILLHREDTNQSDYTIIYNTLQKDPSNSEKEATYYIYRQLRNAEPTD
                     DASAREVINNLFFSEKRYDLGDVGRYRINKKLNLDISPEIKVLTKEDIIAIIQYLIQL
                     LNSKADVDDIDHLSNRRVRTVGEQLYNQFGVGLARMARTIRERMNVRDNEVFTPIDLI
                     NAKTISSVINTFFGTNALSQFMDQTNPLAEITHKRRLSALGPGGLSRERAGFEVRDVH
                     YTHYGRLCPIETPEGPNIGLISSLCVYAKINNLGFIETPYRKVANKTVDLKKEDILYM
                     TAEEEEGKIIAQGNAPLDDKGHFIRAKVKSRKDSDFPVVLPEEVEFMDVAPQQIASIA
                     AALIPFLEHDDANRALMGSNMMRQAVPLLHAEAPIVGTGLEGQLIRDSRTQITAERDG
                     IIEFVDSTCIKVKYDYSDEELFVMFDSPIKEYHISKFQKTNQSTTFDLRPIVKKGERV
                     TKGQILTEGYSTENGELAIGRNLKVAFMPWKGYNYEDAIVINERIVREDIFTSVHVDE
                     YTLEVRETKRGMEELTSDIPNVSEDATKDLDETGIVRIGALIEPGDILIGKITPKGES
                     DPSPEEKLLRAIFGDKAGDVKDASLKASPSLHGVVIGKRLFAKVQKDKKSKNAEKLTM
                     AKLEENFEEETLQLKTILVSKLMELTNGKTSQGVKDFLDADVIAKGNKFSQKQLMDIN
                     YTSVQTTKWTTDAHKNDLIRDVIQNYLRRYKELDAQLKREKYNMTIGDELPSGIIKLA
                     KVYIAKKRKIRVGDKMAGRHGNKGIVSRIVRQEDMPFLEDGTPVDIVLNPLGVPSRMN
                     LGQIFETVLGWAGKELGVKFATPIFDGATIDDLNTWTDKAGIPRYGKTYLYDGGTGER
                     FDQAATVGVIYMLKLGHMVDDKMHARSIGPYSLITQQPLGGKAQFGGQRFGEMEVWAL
                     EAYG"
     gene            complement(3772..4149)
                     /gene="rplL"
                     /locus_tag="PK641_03790"
     CDS             complement(3772..4149)
                     /gene="rplL"
                     /locus_tag="PK641_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HPG55506.1"
                     /translation="MADLKKIAEELVNLTVKDVNELAKILKDEYGIEPAAAAVAVAAP
                     AAGAAAPAAEEKTSFDVALKAAGANKLAIVKLVKELTGLGLKEAKDLVDAAPSVIKEG
                     LSKDDAENMKKQLTEAGAEVELK"
     gene            complement(4197..4724)
                     /gene="rplJ"
                     /locus_tag="PK641_03795"
     CDS             complement(4197..4724)
                     /gene="rplJ"
                     /locus_tag="PK641_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HPG55507.1"
                     /translation="MKKEDKSKVISQLKDALGQYSHFYVTDTSGLNAERTYVLRKNCF
                     QQDVKLVVVKNTLFQKALEATGADYTPLFDVLKGSTSIMFSNNSNAPASLIKQMKKDK
                     ANNDKPVLKAAYVQECFYFGEDQLDTLVSLKSKEQLLGDIIGMLQSPMQNVLSALQSG
                     GSTIQGVLKTLGEKE"
     gene            complement(4744..5436)
                     /gene="rplA"
                     /locus_tag="PK641_03800"
     CDS             complement(4744..5436)
                     /gene="rplA"
                     /locus_tag="PK641_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006254384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HPG55508.1"
                     /translation="MSKQTKNQKLAAAKIEVGKNYSLAEASALVKEISTTKFDASVDI
                     DVRLGIDPRKSNQMVRGVVSLPNGTGKSVRVLALCTPDAEADAKAAGADYVGLDEYIE
                     KIKGGWTDVDVIITMPSIMGKIGALGRVLGPRGLMPNPKSGTVTNEVGKAVREVKQGK
                     IDFKVDKSGIVHTSIGKVSFTPKQIEENAKEFISTLNKLKPSSSKGVYIKSIYLSSTM
                     SKGLKIDPKSAE"
     gene            complement(5455..5898)
                     /gene="rplK"
                     /locus_tag="PK641_03805"
     CDS             complement(5455..5898)
                     /gene="rplK"
                     /locus_tag="PK641_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007755660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HPG55509.1"
                     /translation="MAKEVAGQIKLQVKGGAANPSPPIGPVLGSKGINIMEFCKQFNA
                     RTQDKAGKILPVVITYYSDKSFDFIIKTPPVAIQLLEISKQKSGSAEPNRKKVAEITW
                     DQVRVIAQDKMPDLNCFTVESAMRMVAGTARSMGISIKGEFPASN"
     gene            complement(5964..>6037)
                     /locus_tag="PK641_03810"
     CDS             complement(5964..>6037)
                     /locus_tag="PK641_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HPG55510.1"
                     /translation="VMVMIFGRKTPLELSFMQVEKEQ"
BASE COUNT         1700 a         1221 c         1044 g         2072 t
ORIGIN      
        1 caccgtatgc ttctaatgcc caaacttcca tttcaccaaa acgttgacca ccaaattgtg
       61 ctttaccacc aagaggttgt tgagtaatta aagagtacgg tccgattgaa cgtgcgtgca
      121 tcttatcatc aaccatatgg cctaatttca gcatataaat aacgcctaca gttgcagctt
      181 ggtcgaagcg ttcacctgtt ccaccatcat acaagtaagt tttaccataa cgaggaatgc
      241 cggctttatc tgtccatgta ttaaggtcat ctatagttgc gccatcaaaa attggtgtag
      301 caaatttaac tcctaattct ttgccagccc agcctaaaac agtttcaaaa atctgtccaa
      361 ggttcatacg agaaggcaca cccagagggt ttaggacaat gtcaacaggc gtaccatctt
      421 ctaagaatgg catatcttct tgacgtacaa ttcttgatac tatacccttg ttaccatggc
      481 gtcctgccat cttatcaccg acgcgaatct tacgtttctt agcaatataa actttggcta
      541 atttgataat acctgaaggt aattcatctc caatagtcat attgtatttt tcgcgtttaa
      601 gctgtgcgtc tagttcttta tagcggcgta aatagttttg aattacatcg cgaatcaaat
      661 cgtttttgtg tgcatcagtt gtccacttgg ttgtttgtac gcttgtgtaa ttaatgtcca
      721 ttaattgctt ttgtgagaac ttattgcctt ttgcaataac atctgcatcc aaaaagtctt
      781 taactccttg cgaagttttt ccgtttgtaa gctccatcaa tttactaact aaaatagttt
      841 tcaattgtaa agtttcttct tcgaagtttt cttctaattt ggccattgtt aatttctcgg
      901 cattctttga tttcttgtcc ttttgaactt ttgcgaacaa gcgtttgcca ataactacac
      961 catgtaatga aggtgatgct tttaaagatg cgtctttaac gtcaccagcc ttatcaccaa
     1021 atatagcacg taacaatttt tcttcaggag aaggatctga ctcgccttta ggagtgattt
     1081 taccaattag aatatcacca ggttcgatta gtgcaccaat gcgaactata cctgtttcat
     1141 ctaaatcttt tgttgcatct tcgcttacgt ttggaatatc agaggttaat tcctccatac
     1201 cgcgttttgt ttcacgaact tccaatgtgt attcgtcaac atgcacagaa gtgaaaatat
     1261 cttcacgtac aatgcgctcg ttaataacga tggcatcctc gtaattatat cctttccaag
     1321 gcatgaatgc aacttttaaa ttgcgtccga ttgccaattc accattctcc gtagaataac
     1381 cttctgtcag aatctgacct tttgtgacac gctctccttt tttaacaata ggacgtaagt
     1441 caaaggttgt actttgattg gtcttttgaa atttagaaat atgatattct tttataggac
     1501 tatcgaacat gacaaataat tcttcgtctg agtaatcata ttttacttta atgcaagttg
     1561 aatcaacaaa ttcaataata ccatcacgtt cagctgtaat ctgtgtgcgt gaatcgcgaa
     1621 ttaattgacc ttccaatcct gttccaacaa taggagcttc ggcatgtaac aaaggtactg
     1681 cctgtctcat catgtttgaa cccattaaag cacggttagc atcatcgtgt tccaagaatg
     1741 gaattaatgc agctgcaata gaagcaatct gttgtggagc tacgtccatg aattctactt
     1801 cttccggtaa aacaacgggg aaatcactat ctttgcgaga tttaaccttg gcacgtataa
     1861 aatgaccttt gtcgtcaaga ggagcattac cttgagcgat aatcttgccc tcttcttctt
     1921 cggcggtcat atacaatatg tcttcttttt ttaaatcaac tgttttatta gcaaccttac
     1981 gatatggagt ttcgataaat ccaagattat taatttttgc atatacgcat aaagacgaaa
     2041 tcaaaccgat gtttggacct tcaggggttt caatcgggca aagacgtcca taatgtgtat
     2101 agtgtacgtc acgaacctca aatcctgcac gttcacgaga caaaccgcca ggtcctaatg
     2161 ctgatagacg gcgtttgtgt gtgatctcag ccaatggatt ggtttgatcc atgaattgag
     2221 aaagtgcatt tgttccaaag aacgtgttga ttactgacga tattgttttt gcattaatca
     2281 agtcaatagg agtaaatact tcgttgtcgc gtacattcat acgttcacga attgtgcgtg
     2341 ccatacgtgc aagtccaact ccaaattggt tgtataattg ctctccaaca gtacgtacac
     2401 gacgattact taagtgatcg atatcatcaa cgtctgcctt tgaatttaaa agttgtatta
     2461 aatattgaat aatagcaata atatcctcct tagttaaaac tttaatttca ggagatatat
     2521 ccagattcaa ttttttgttt atacgataac gacctacatc acctaaatcg tatctctttt
     2581 cagagaagaa caggttgtta ataacctcgc gtgcgctagc atcgtctgta ggttctgcgt
     2641 tacgtaattg acgatatata taatatgttg cttctttttc cgaattactt ggatcttttt
     2701 gcaatgtatt atatattatg gtgtaatcag attggtttgt atcttctctg tgcaacaaaa
     2761 ttgttgaaac atttgcttct agtatagtgt cgatgtgttc tgttgacaaa actgtttcac
     2821 gatcgattat aacttcgtta cgatcaattg aaacaacttc gccggtatct tcatcaacaa
     2881 aatcttcaac ccatgttttc aacacgcgtg cagccaatgt acgaccaaca gatttcttta
     2941 aactcgcctt tgatacttta acttcttctg caaggttgaa aatttccaaa atatctttgt
     3001 cgctttcaaa tccgattgct cttaaaagtg tagtaacagg caattttttc tttctgtcga
     3061 tgtatgcgta catgacattg ttaatgtctg tcgtgaattc tatccacgaa ccgcggaacg
     3121 gtataattct tgccgaatat aactttgtac cgtttgcgtg catgcttttt ccaaagaaaa
     3181 caccgggtga acgatgtaac tgtgaaacga ttacgcgttc agctccgtta atgacaaaag
     3241 ttcccttttc tgtcatataa gggattggcc ccaaatatac gtcttgtatt acagtttcga
     3301 agtcttcatg atcgggatcg gtgcagtaca atttcagctt tgcctttaaa gggacactgt
     3361 aagttaatcc acggtctatg cattcttgaa tgctatacct tggtggatct acatagtagt
     3421 ccaaaaattc aaggatgaaa ttattacgag tatctgctat tggaaaattt tccgcaaata
     3481 ctttatacaa accttcgttc ttgcgttttt cgggcggggt atcaagttgg aaaaagtcct
     3541 ggaacgactt caattgtacc tccaggaaat ctgggtattc caacggattt ttaatggaag
     3601 aaaaatttat tctttggtta ctcatacgag aggtctatca acatggttaa tgaactcgtg
     3661 attgccttat tctcggtgga atagaacacc actttattta taaacataaa aaggcttaga
     3721 accttatttc taaggttcta aaccgaattt cctgattaac aggatgttag tttacttcaa
     3781 ttcaacttca gcaccagctt ctgttaattg ttttttcatg ttctcagcat cgtcttttga
     3841 caatccttct ttgattactg aaggagcagc atcaactaaa tctttagctt ctttcaagcc
     3901 aagacctgtc aattctttta ctaattttac gattgccaat ttatttgcac cagcggcttt
     3961 caaagcaaca tcaaaagatg ttttttcttc agcagcagga gcagcagcac cagcagcagg
     4021 agcagctacg gctacagcag cagctgcagg ttcgatacca tattcgtctt tcaaaatttt
     4081 agctagttca tttacatctt ttactgtcaa gtttactaac tcttctgcaa tttttttcaa
     4141 atctgccatt gttttatgat ttaaaataag ttactaataa attaattata aattttttat
     4201 tctttctcgc ctaatgtttt cagcacgcct tgtattgtac ttccgcctga ttgcaaagcc
     4261 gatagtacgt tttgcatagg agactgtaac ataccgataa tatcgccgag aagttgttcc
     4321 tttgatttca aagaaaccaa tgtatctaat tggtcttcgc caaaatagaa acattcttgt
     4381 acataagctg ctttcaagac aggtttgtca ttgttagcct tatctttctt catttgtttg
     4441 ataagactag caggtgcatt actgttattt gaaaacatga tcgatgtgga accctttaaa
     4501 acgtcgaata acggagtata gtccgcaccc gtagcttcaa gagctttttg gaacaaggtg
     4561 ttttttacaa ctaccaattt tacgtcttgt tgaaagcaat tttttctaag tacgtaagta
     4621 cgttcagcgt ttaagcctga tgtatctgta acatagaaat ggctatattg tcctagcgca
     4681 tctttcaatt gacttattac tttgctttta tcttcctttt tcataacatt gtttttttta
     4741 aagttattca gcagatttag ggtcgatttt cagacctttg ctcattgtgc tggaaagata
     4801 aatgctctta atataaacac ctttagaaga agatggtttc agtttgttca acgttgaaat
     4861 aaactctttt gcattctctt caatctgttt aggagtgaaa gatactttgc cgatagaggt
     4921 atgtacaata cctgatttat caactttgaa atctatttta ccttgtttta cttctcttac
     4981 agccttgcca acttcatttg taactgtgcc gctcttaggg tttggcatta agccacgagg
     5041 gcctaaaaca cgacctagag caccaatttt acccatgata gaaggcattg tgatgataac
     5101 atcaacatct gtccatccac ctttaatctt ttcgatgtac tcgtcaagtc cgacataatc
     5161 ggcaccagct gctttcgcat ctgcttccgc gtcgggtgta cataaagcaa gaaccctaac
     5221 tgatttacct gttccattag gcaaagagac aacgcctctt accatttggt tagatttacg
     5281 agggtcaata cccaaacgaa cgtctatatc tacagacgcg tcgaattttg tggtacttat
     5341 ttcctttacc aaagcagatg cttctgcaag tgaatagttt ttacctactt caatctttgc
     5401 agcagccaat ttttggtttt ttgtctgttt actcattttt taaaaattga agtgttagtt
     5461 acttgcaggg aattctcctt ttatagaaat acccatgctt cttgctgttc cggctaccat
     5521 acgcattgca gattcaactg taaaacagtt taaatcgggc attttatctt gagcgattac
     5581 tcttacttga tcccaagtaa tttcagcaac ttttttacgg ttcggttctg cagatccgct
     5641 tttttgtttg gatatttcca acaattggat tgctaccgga ggcgttttga tgataaaatc
     5701 gaacgattta tctgagtaat acgtaataac tacaggtaat attttacctg ctttgtcttg
     5761 tgttctggca ttaaactgtt tgcaaaactc cattatattg atacccttag aacctaagac
     5821 aggaccaata ggaggagacg gatttgctgc accgccttta acttgcaatt tgatttgtcc
     5881 agcaacttct tttgccatat tatcaaatac taattgttaa taaaaaaatg atatgcgaat
     5941 cggaacaatt aaagcgtaac cttttactgt tctttttcta cttgcataaa gctcaattca
     6001 agcggggttt tccgcccaaa aatcatgacc ataacct
//
LOCUS       DAOPID010000079         6002 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_17308_length_6002_cov_5.353960, whole genome shotgun sequence.
ACCESSION   DAOPID010000079 DAOPID010000000
VERSION     DAOPID010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17308_length_6002_cov_5.353960"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..304
                     /locus_tag="PK641_03815"
     CDS             128..304
                     /locus_tag="PK641_03815"
                     /inference="COORDINATES: protein motif:HMM:NF013024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL15m family ribosomal protein"
                     /protein_id="HPG55511.1"
                     /translation="MEKIGIEELIEAGFVSSKHKVKILAKGEITSKLEVSAHAFSKTA
                     VEEIEKAGGTVVTL"
     gene            314..1657
                     /gene="secY"
                     /locus_tag="PK641_03820"
     CDS             314..1657
                     /gene="secY"
                     /locus_tag="PK641_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPG55512.1"
                     /translation="MKKFIETIRNIFKIEELKSRLAITFLLVIVYRFGSYIALPGIDT
                     TQLSELSKQTSGGLMMLLDMFSGGAFSNASIFALGIMPYISASIVVQLLGMAFPYFQK
                     LQREGESGRQRMNQITRYLTILILLLQGPAYLINLRMQVGSVVPEGAFFMITSVLILA
                     AGSMFVLWLGERITEKGVGNGISFIILVGIIARFPRAIVGEFSSRLHEAAGGLVLFLA
                     EIVFLLVVIGFSVLLVQGTRKVPVQYAKRVVGNQQYGGVRQYIPLKVNAANVMPIIFA
                     QAIMFIPASLSGLFASDGEVSNFAMTFMDNNSFWYNCVFFLLIIAFTYFYTAVTVNPT
                     QMAEEMKRNNGFIPGIKPGKKTAEYLDTIMSRITLPGSIFLGIVAILPAFAGSEWLGV
                     SASFAQFFGGTSLLIMVGVVLDTLQQIESYLLMRHYDGLMKGGKIKGRSGSIAAY"
     gene            1666..2445
                     /gene="map"
                     /locus_tag="PK641_03825"
     CDS             1666..2445
                     /gene="map"
                     /locus_tag="PK641_03825"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPG55513.1"
                     /translation="MIYLKTPEEIAILKKSNRLVGKTLAEVAKAIEIGVTTLELDKIA
                     EDFIRANGATPGFLGYNGFPNTLCISVNEQVVHGIPSKYTLKDGDIVSVDCGTIIDGF
                     YGDSAFTFCVGNVDEKTQKLLKTTREALYKGIEIAVHGNRIGDVGYAIQHHCEAAGFS
                     VVREMVGHGVGRNMHEAPEVPNYGKRGQGILLKEGMTIAIEPMINMGNKGIVFEKDGW
                     TVRTIDRKPSAHFEHTIAIHKGEAEILSSFDYVDEVLSHKS"
     gene            2446..2664
                     /gene="infA"
                     /locus_tag="PK641_03830"
     CDS             2446..2664
                     /gene="infA"
                     /locus_tag="PK641_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPG55514.1"
                     /translation="MAKQAAIEQDGTIVEALSNAMFRVELENGHEITAHISGKMRMHY
                     IRILPGDKVRVEMSPYDLSKGRISFRYK"
     gene            2680..2796
                     /gene="rpmJ"
                     /locus_tag="PK641_03835"
     CDS             2680..2796
                     /gene="rpmJ"
                     /locus_tag="PK641_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HPG55515.1"
                     /translation="MKVRTSVKKRSDDCKIVRRKGRLYVINKKDPKMKQRQG"
     gene            2826..3206
                     /gene="rpsM"
                     /locus_tag="PK641_03840"
     CDS             2826..3206
                     /gene="rpsM"
                     /locus_tag="PK641_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPG55516.1"
                     /translation="MAIRIAGVDLPQNKRGEIGLTYIYGIGRNSANKILSEAGVDKDI
                     QVKDWTDDQAAKIRDILSSKFKVEGDLRSEVQLNIKRLMDIGCYRGIRHRLGLPLRGQ
                     STKNNARTRKGKKKTVANKKKATK"
     gene            3237..3626
                     /gene="rpsK"
                     /locus_tag="PK641_03845"
     CDS             3237..3626
                     /gene="rpsK"
                     /locus_tag="PK641_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPG55517.1"
                     /translation="MAKKTAATKKRVVKIDGTGQAHVCSSFNNIIVTITNAQGEVISW
                     SSAGKMGFRGSKKNTPYAAQMAAQDCAKVAFDLGLRKVKAYVKGPGNGRESAIRTIHG
                     AGIEVTEIVDVTPLPHNGCRPPKRRRV"
     gene            3655..4260
                     /gene="rpsD"
                     /locus_tag="PK641_03850"
     CDS             3655..4260
                     /gene="rpsD"
                     /locus_tag="PK641_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPG55518.1"
                     /translation="MARYTGPKTKIARKFGEAIFGADKVLSKKNYPPGQHGNSRKRKT
                     SEYGVQLKEKQKVKYTYGVLEKQFSNLFDRASRTKGITGEVLLQLLESRLDNVVFRLG
                     MAPSRSAARQLVGHKHVVVNGEVVNIPSFQVKQGDVVAVREKSKSLEVIVNALSGFNH
                     SKYPWLEFDESSMSGKFLHMPDRADIPENIKEQLIVELYSK"
     gene            4270..5262
                     /locus_tag="PK641_03855"
     CDS             4270..5262
                     /locus_tag="PK641_03855"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPG55519.1"
                     /translation="MAILPFQKPEKVIMLEADTFHGKFEFRPLEPGFGITIGNALRRI
                     LLSSLEGFAITSLRIDGVQHEFATVPGIMEDVTNIILALKQVRFKQLVEGVDSEKVTI
                     DVADTTTFKAGNIGKHLTAFEVLNPDLLICTMDPKASFHIELTINKGRGYVPADENRG
                     LVQGLDVIPIDSIYTPIVNVKYSVENYRVEQKTDYEKLVIEIDTDGSIHPKEALKESA
                     KILIHHFMLFSDEKITIDFNDSEGGEEFDEDALHMRQLLKTKLVDLDLSVRALNCLKA
                     ADVETLAELVSFNRNDLLKFRNFGKKSLVELDAKLASLNLSFGMDISKYKLDKE"
     gene            5269..5778
                     /gene="rplQ"
                     /locus_tag="PK641_03860"
     CDS             5269..5778
                     /gene="rplQ"
                     /locus_tag="PK641_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007747966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HPG55520.1"
                     /translation="MRHNKKFNHLGRKANHRNAMLSNMAVSLIKYKKINTTLAKAKEL
                     RKFVEPLITKSKEDTTHSRRIVFSALQNKEAVTVLFGEISQRIADRPGGYTRILKTGF
                     RLGDAAQMCFIEFVDYNENMLKDTASKKKTKTRRAGAKKAVEAKVETAEVVEDAIKEN
                     PVAETPAAE"
     gene            5873..>6002
                     /gene="pyrH"
                     /locus_tag="PK641_03865"
     CDS             5873..>6002
                     /gene="pyrH"
                     /locus_tag="PK641_03865"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668426.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPG55521.1"
                     /translation="MLTYKRILLKLSGESLMGEQGYGIDSKRLSQYAEQISEIASMG"
BASE COUNT         2048 a          868 c         1251 g         1835 t
ORIGIN      
        1 ggcctttatt ttttacgttt tggatagcaa aaaaaactcc ccaaacaagc caacttaaaa
       61 ttacaattgc cagtccatta attttaatgt ttggcagcca tattgatact attatagcga
      121 gaaaaaaatg gaaaaaatcg gcatcgaaga attaattgaa gctggttttg tatcttcaaa
      181 gcacaaagta aaaattttag caaaaggcga aataacatct aaattagaag tttctgctca
      241 tgcgttttct aaaactgcag tagaagaaat tgaaaaagca ggtggtacgg tggtaacttt
      301 ataatccgtt atcatgaaaa aatttattga aacaatacgg aatatattta agatagagga
      361 gcttaaaagt agactagcca ttacatttct tttggttata gtttatcgtt ttggttccta
      421 tatcgctctt ccgggtatag acacaacaca actttctgaa ctgagtaaac aaactagtgg
      481 aggtttaatg atgttgttgg atatgttctc tggtggtgct ttttctaatg catcaatttt
      541 tgcattgggg attatgccgt atatctcggc atcaatcgta gtgcaattgt taggtatggc
      601 tttcccatat tttcaaaaat tgcaacgtga aggagaaagc ggtcgccaac gcatgaatca
      661 aatcactcgc tatttgacta ttttgatttt attgcttcaa ggacctgcat atttgatcaa
      721 tttaagaatg caagtaggat cagttgttcc tgaaggtgca ttctttatga tcacatctgt
      781 attgattttg gcagccggta gtatgtttgt actatggtta ggcgaacgta ttacagaaaa
      841 gggtgtaggt aatggtattt catttatcat cttagtaggt attatagcgc gttttccaag
      901 agcaattgta ggtgagtttt catctcgttt gcacgaggct gccggtggtt tagtattgtt
      961 cttggcggag atagtattct tgttagtagt aataggattt agtgtattat tagttcaagg
     1021 tactcgtaaa gttcctgtac aatatgcaaa acgtgttgta ggcaatcaac aatatggtgg
     1081 cgttcgtcaa tacattccat taaaagtgaa tgcagcaaat gtaatgccta tcatttttgc
     1141 tcaagcgatt atgtttattc cggcatcttt atctggttta tttgcgtcag atggagaagt
     1201 tagtaatttt gccatgactt ttatggataa taacagtttt tggtataatt gtgttttctt
     1261 tttactgatt atcgcattta cgtattttta tacagcagta acagtaaatc ctactcaaat
     1321 ggcagaagag atgaaacgta ataatggttt tattccgggt attaaaccag gaaagaaaac
     1381 agctgagtat ttggatacaa ttatgtctag aattacatta ccaggttcta tattcttggg
     1441 cattgtagct attttgcctg cgtttgcggg atctgaatgg ttaggcgtaa gcgcatcttt
     1501 tgctcaattt tttggaggaa catctctttt gattatggta ggtgttgttt tagatacatt
     1561 acaacaaatc gaaagctatt tattaatgcg tcattatgat ggattgatga aaggtggaaa
     1621 aataaaaggg agaagtggct cgatagccgc atattaaaaa taaagatgat atatcttaaa
     1681 actccggaag agattgcaat attaaaaaaa agcaatagac ttgtcggaaa aacattggca
     1741 gaagtcgcaa aggcgattga aattggagta acaacacttg aattagataa aattgcagag
     1801 gattttattc gtgcaaatgg tgcaactcct ggctttttag gttataatgg tttcccaaat
     1861 actttatgta tttccgtaaa tgagcaagtt gttcatggaa taccatctaa atatactttg
     1921 aaagacggtg atatagtttc ggtagattgc ggaacaataa ttgatggatt ttatggagac
     1981 tcagcattta cattttgtgt tggaaatgtt gatgaaaaga cacaaaaact tctaaagact
     2041 actcgtgaag ctttatacaa aggtatagaa atagctgttc atgggaaccg aattggagat
     2101 gtgggatatg caattcagca tcattgtgag gcagccggtt tctctgtagt aagagaaatg
     2161 gtaggacatg gagtaggacg caatatgcat gaagctcctg aggtaccaaa ttatggaaaa
     2221 cgtggtcagg gtatactctt aaaagaagga atgacaattg ctattgagcc aatgataaat
     2281 atgggtaaca aaggtattgt ttttgaaaaa gatggttgga ccgttcgtac aatagataga
     2341 aaaccatccg ctcattttga acatacaatt gcaattcata aaggagaagc agaaatatta
     2401 tcatcgttcg attatgtaga tgaagtttta agtcacaaat cataaatggc aaaacaagca
     2461 gcaatagagc aagatggaac catcgtagaa gcattgtcaa acgcaatgtt tcgagtagaa
     2521 ttggaaaatg ggcatgaaat tacagctcac atatcgggta agatgcgtat gcattatatt
     2581 cgtatattac caggtgataa ggtgcgtgtt gaaatgtcac cttatgattt gagtaaagga
     2641 agaatttcgt tcagatacaa ataatttaaa ttttaaataa tgaaagtcag aacatctgtt
     2701 aaaaaacgca gtgatgattg caaaatcgtc agaagaaaag gtcgtcttta tgtgatcaac
     2761 aaaaaagatc caaagatgaa acaacgtcaa ggttaataat ttaccaaaat catataaaat
     2821 agtttatggc tataagaatt gcaggagtag atttacctca gaataagaga ggtgaaatag
     2881 gtttaaccta tatctacgga ataggaagaa acagcgccaa caaaatattg tcggaagctg
     2941 gtgtcgacaa agacattcaa gttaaagatt ggacagatga ccaagcggca aaaatccgtg
     3001 acatcctttc ctctaaattt aaagtggaag gtgatttgcg ttctgaggta caattgaaca
     3061 ttaaacgttt aatggatatt ggctgctatc gcggtattcg tcatcgttta ggtttaccat
     3121 tgcgtggcca aagtacaaaa aacaatgcac gtacgcgcaa aggaaagaag aaaacagttg
     3181 caaacaagaa aaaggcaact aagtaattaa ttgtagaaca gaaaataaat taagatatgg
     3241 caaaaaaaac tgccgctact aaaaaaagag tagtgaaaat agatggaact ggtcaagccc
     3301 acgtatgttc atcatttaac aatataattg ttacgattac aaatgcccaa ggagaagtaa
     3361 tttcttggtc gtctgcaggt aaaatgggtt ttcgcggttc aaaaaagaat acaccgtatg
     3421 cagctcaaat ggcagcacaa gattgtgcaa aggttgcatt tgatttgggt ctgcgtaaag
     3481 taaaagctta cgtaaaaggt ccgggtaatg gaagagaatc tgctattcgt acaattcatg
     3541 gagcaggtat agaggtaaca gaaattgtag atgtaacacc gctacctcac aatggttgcc
     3601 gtcctcctaa aagacgtaga gtataataag ttaaaccgct aaaaatagaa taaaatggca
     3661 agatatacag gacctaaaac aaaaatagcg cgtaagtttg gagaagcaat atttggcgct
     3721 gacaaagttt tgtctaagaa gaattatcct cccggtcaac acggtaattc tcgtaaacgt
     3781 aaaacttctg aatacggtgt tcagttgaaa gaaaaacaaa aagttaaata tacttatggt
     3841 gttttggaaa aacaattctc aaatttgttt gacagagcat ctcgcacaaa aggtattact
     3901 ggtgaggttt tattacaatt gttggaatct cgtttagaca atgttgtatt ccgtttagga
     3961 atggctcctt ctcgttcggc tgcacgtcaa ttagtagggc acaaacatgt tgttgttaat
     4021 ggagaagttg taaatattcc ttcttttcaa gtaaaacaag gtgatgttgt tgcagttcgc
     4081 gaaaaatcta aatcattaga ggttattgtg aatgcattaa gtggatttaa tcatagtaaa
     4141 tacccatggt tagagtttga tgaatcttct atgtctggaa aattcttaca catgccagat
     4201 cgtgcagata ttccggaaaa tattaaggaa caattaatcg tcgaacttta ttctaaataa
     4261 tgtagaatta tggctatatt accatttcaa aaaccagaaa aagttatcat gttggaggca
     4321 gacacgttcc atggtaagtt tgaattcaga ccattggagc cgggcttcgg tattactatc
     4381 ggtaatgctt tacgccgcat cttgctttct tcattggagg gctttgccat cacatcgtta
     4441 aggattgatg gagtacaaca cgaatttgct acagttcctg gaataatgga ggatgtaacg
     4501 aatattatct tagctttaaa acaagttcgt tttaaacaac ttgttgaagg tgtggatagt
     4561 gaaaaagtta caatcgacgt agcagataca accacattta aggcaggtaa tattggtaaa
     4621 catttaactg cttttgaggt attgaatccg gatcttttaa tatgtacaat ggatcctaag
     4681 gcatctttcc atatcgaatt aacgattaac aaaggacgtg gatatgttcc tgctgatgaa
     4741 aatcgtggct tagttcaagg attagatgtt ataccaatag actctattta cacacctatt
     4801 gttaatgtta aatattctgt tgaaaactac cgtgtagaac aaaaaacaga ctatgaaaaa
     4861 ttagtaatag aaattgatac agatggttct attcatccta aggaagcttt gaaagaatct
     4921 gcaaaaatat tgattcatca ctttatgttg ttctctgatg agaaaataac aattgatttc
     4981 aatgattcag agggtggaga agaatttgat gaagatgcat tgcatatgcg tcagttgttg
     5041 aaaactaagt tagttgatct tgatttgtca gtacgtgcat taaattgctt aaaggctgct
     5101 gatgttgaaa ctttagctga gttggtttct ttcaacagaa atgatttgtt gaaattccgc
     5161 aattttggta agaaatcgtt agtagagtta gatgctaaat tggcaagttt gaacttgtcg
     5221 tttggtatgg acatttctaa gtataaatta gataaagagt aatacgagat gagacataat
     5281 aagaaattta atcatttggg aagaaaagcg aatcatcgca atgcaatgtt gtcaaacatg
     5341 gctgtttccc taattaagta taagaagatc aatactacgc tcgcaaaggc aaaagaattg
     5401 cgtaagtttg ttgagccgtt gattacaaaa tcaaaagagg atacaactca ttctagaaga
     5461 atcgtttttt cagctttaca gaataaggaa gcagttacag ttcttttcgg tgaaatttct
     5521 caacgcatag ccgatcgtcc cggtggttat acacgtattt tgaaaacagg cttccgttta
     5581 ggagatgctg ctcaaatgtg tttcattgag tttgttgatt acaacgaaaa catgttgaaa
     5641 gatacagcat caaagaagaa aactaaaaca cgtcgtgctg gtgcaaagaa agcagtagaa
     5701 gcaaaagttg aaacagcaga agtagtagag gatgcaatta aagaaaatcc tgtagcagaa
     5761 acacctgctg ctgaataact ttatttatat tataaaaagg gataagactt aatcttatcc
     5821 ctttttttgt gtaaataatt atgctatatt tgcacactgc aaattaatta atatgcttac
     5881 atataaaagg atcttattaa aattgagtgg tgagtcgctt atgggcgaac aaggctatgg
     5941 aatagattca aagcgtttgt ctcaatatgc tgaacaaatc agtgaaatag cttcaatggg
     6001 gg
//
LOCUS       DAOPID010000080         5987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_17367_length_5987_cov_4.756912, whole genome shotgun sequence.
ACCESSION   DAOPID010000080 DAOPID010000000
VERSION     DAOPID010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 5987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5987
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17367_length_5987_cov_4.756912"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..785
                     /locus_tag="PK641_03870"
     CDS             51..785
                     /locus_tag="PK641_03870"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPG55522.1"
                     /translation="MDKADTRRGKPTVHKRWNDNTAILSGDAMLIEAYKLIADCPPNV
                     LKKVLDVFSQTALEVCDGQQYDMEFETRTDVSMVDYIEMIRLKTAVLLAGSLKIGAII
                     GGANDQDANCLYNFGINIGIAFQLRDDYLDVFGDKLVFGKNIGGDILANKKTFLLIEA
                     SSLAQAETKAELENWVANNDPKHASDKIAAITEIYKKTGVDFRCEEKMNFYFNESVES
                     IKNLQCSDNKNIVLLNFAKSLLCRKK"
     gene            872..1600
                     /locus_tag="PK641_03875"
     CDS             872..1600
                     /locus_tag="PK641_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55523.1"
                     /translation="MPYRRLPNTDAARMRALQVARKQYELKGAENVPYSSKIVHEISA
                     FIPFFERAIAEYKTSVETQFAKNQKYQNQVRMVRMYISHFIQVLNLSCQRNEIKPEKK
                     LLYKLLPDNYSVPDLSTEMLLIEWGKNIIDGESERTRSGGAPIYNPAISKVSVHYDQF
                     CDSFQTKKVSKKNTDRYLESVQKLRIQADALILNLWDQIEKAYAELGTEEMQNRAKEF
                     GIVYYLRRKERQALGLPVSGDEDK"
     gene            complement(1537..2469)
                     /locus_tag="PK641_03880"
     CDS             complement(1537..2469)
                     /locus_tag="PK641_03880"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HPG55524.1"
                     /translation="MKLIGIFDKSNNFIKSLVIILVPCFLFCFSYTIILLIAKLSGTD
                     LNVIATDKSWMEQVQLFTSISVFILGAVVCASLLDKHPFSYISANKSVKIPIYILAIF
                     CLFFSVPLINFTTEINSQIHLPDALKELEDYLRSMEEASQNMTKLFLSGNGFKDYLFT
                     IFLMALIPAVGEEFFFRGILQSYLKSLTNNNHAAIWLTAFIFSLIHFQFLGFIPRMLL
                     GALLGYLLVWTGSIWVPIVAHFTNNALAVTGYWLLQHKYIDVDIDNVGVGDDLIINVL
                     LPTAILGILLFLLRHLSSSPETGNPKAWRSLRRK"
     gene            complement(2466..3368)
                     /locus_tag="PK641_03885"
     CDS             complement(2466..3368)
                     /locus_tag="PK641_03885"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HPG55525.1"
                     /translation="MIQAPFLHIGSHICILSPSGVVNPDFVWNAKDVLESWGLIVTIG
                     KSVFAANGRFAGTDEERIADIQSAIDDNNCDAILCSRGGYGLARIIDKIDFSKLQERP
                     KWILGFSDITLIHSALSLHGITSIHSIMTKNLALQAKEEPALRLKQLLFGELPSYSIG
                     YERFNKKGTALGNLVGGNLSVIYGLRGTAFDLQAKNNILFIEDVCEPLYHIDRMIQNI
                     RLGGIFNQISGLVVGQFTDIQEDTSFPDGVYGIIENAVKDYSFPVCYGFNAGHTDYNL
                     PLILGHKTSLSVSDNGDSILTQNI"
     gene            complement(3365..4918)
                     /locus_tag="PK641_03890"
     CDS             complement(3365..4918)
                     /locus_tag="PK641_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HPG55526.1"
                     /translation="MKQIHILWADDEIDLLRPHIIFLESKGYSVTAVNSGQDAIDACN
                     ENNFEVVFLDENMPGVSGLETLLQIKKQHPELPVIMITKSEEEDIMEQAIGQKISDYL
                     IKPVNPNQILMAIKKVVQAQDIISQKTTTNYQSEFMRISMQINDSFNTDDWKKVYKNL
                     VFWELELEQANNNMDEMLQMQKNEANATFVKFIKKNYENWFKENNARPLMSPDIFKTK
                     VFPLLDNGEKVFFVLIDNLRLDQWRVMQKELAKWFNFTDDDTFFSILPTATQYSRNAI
                     FSGLMPSQIRKMYPELWVDEEEDEGKNMNEEALIQTQLDRFRKKYKFSYNKINTNQAG
                     EKLNEGIKQLLDNPLNVVVFNFVDMLSHARTEVKLFRELVPDEAAYRSLVLSWFKHSS
                     IAEFFKILSESGVSVVLTTDHGAIRVKNPVKVIGDKNTNVNLRYKVGKNLNYDKKDFL
                     VINKPELIGLPSPNISSTYVFSSNDDFIAYPNNYNYYVSYYKDTFQHGGISLEEMIIP
                     IISMKAKKQ"
     gene            complement(4983..>5987)
                     /locus_tag="PK641_03895"
     CDS             complement(4983..>5987)
                     /locus_tag="PK641_03895"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPG55527.1"
                     /translation="NKMDELLEKGHSLIDRYYKFIKILIMVCAYAYLAYVLVNFKGYN
                     EFFNSFKNISAFRTILLVLAVLLIPCNWLLESMKWQHLSQKLQPVSLCKAYKSVLMGL
                     TVGFFTPNRIGDPLGRVLILNACNRTKGIVLSVIGVLGQTFSTLVCGLTAGILLIVFS
                     PFESIKDSLNIQIASILLIIAFISLYFTLPIWGKILFQRNRFKRLRSLLEAISYINPR
                     SLFYVTFLSLIRYIVYCSQYYFLLRFFDVELSLTDAAMILPLNYLMVSVTPTIAFSEL
                     GVRGSYAVLFIGAFASNTVGAALAGMSVWFINYVIPMMVGSIFVGKMKKQGESTTANL
                     "
BASE COUNT         1993 a         1034 c         1001 g         1959 t
ORIGIN      
        1 tccaaagcag gaacgcgtag cgacaccgct gaaagtagct gcaataaatt atggacaaag
       61 cagatacgcg tagaggtaaa cccacagtgc ataaacgttg gaatgataat actgcaatat
      121 tatccggtga tgcaatgctt attgaggcat acaagctaat agccgattgt cctcctaatg
      181 tattaaagaa agttcttgat gtgttttctc agactgcatt agaagtttgc gatggtcagc
      241 agtatgatat ggagttcgaa acacgtacgg atgtttctat ggtagactat atagaaatga
      301 tacgcctaaa aacagctgtg ctacttgcag gaagtttaaa aatcggtgct attataggcg
      361 gtgccaatga ccaagatgcc aattgtttat ataattttgg tataaacata ggtatagcat
      421 ttcaattacg tgatgattat ctggatgtat ttggagataa acttgttttc ggtaaaaata
      481 taggaggcga catacttgct aataaaaaga catttttact tattgaagct agttctttag
      541 cacaagcaga gaccaaggct gaattagaaa attgggtagc aaataatgat ccaaaacatg
      601 cttccgataa aattgctgca atcactgaaa tatacaaaaa aaccggagtg gattttcggt
      661 gcgaagagaa gatgaatttt tattttaatg aatcagtcga atctataaag aatttacaat
      721 gctctgataa taagaatatt gtactactaa attttgctaa atccttgctt tgtagaaaaa
      781 aataaatatt ttttttgttg aaaagttttt tcgtttcaat tattgtttct atttttacct
      841 cggtaaatca ataatttaat cgaaaaaagc catgccatat cgacgtttac ctaatactga
      901 cgcagctcga atgagagctc ttcaggttgc tcgcaagcaa tatgaattaa aaggtgcaga
      961 aaatgttcct tactcttcaa agatagtgca tgagattagt gcctttattc ctttttttga
     1021 gagagcgata gctgaatata aaacttctgt tgaaactcag tttgcaaaaa atcaaaagta
     1081 ccagaatcaa gtgcgaatgg ttcgtatgta tatctcacat tttattcaag tattgaatct
     1141 atcttgccaa cgtaacgaga ttaaacctga gaagaaacta ttatacaaac tattacctga
     1201 taattattca gttcctgatt tgagtacaga gatgttgctg attgaatggg gcaagaatat
     1261 tatcgatggt gaaagcgagc gtacacgcag tggtggagct cctatttata atccagcaat
     1321 aagcaaagtt tcagttcatt atgatcaatt ttgtgatagt ttccaaacaa agaaagtatc
     1381 aaaaaagaat acagacagat atttggaatc agttcaaaaa ttgcgtattc aggcagatgc
     1441 acttatctta aatctttggg atcaaataga gaaagcttat gccgaattag ggaccgaaga
     1501 aatgcaaaat agagccaaag aatttggtat tgtttattat ttacgtcgta aggagcgcca
     1561 agctttggga ttacctgttt caggtgatga agataaatga cgtaataaaa atagcaatat
     1621 tcctaaaata gctgttggca atagcacatt gattattaaa tcatcaccca cacctacatt
     1681 gtctatatca acatctatat atttgtgttg tagtagccaa taacccgtaa ctgccaatgc
     1741 attatttgta aaatgagcaa ctataggaac ccaaattgat cctgtccaga ctaataaata
     1801 accgagtagt gctcctagca acatacgcgg tatgaatcct aagaactgaa aatgtattaa
     1861 actgaatata aaagctgtca accaaatagc tgcgtgatta ttatttgtta ggctttttag
     1921 atacgattgt aagatccccc tgaaaaaaaa ctcctcccca acagcaggta ttaaagccat
     1981 taaaaatatt gtaaatagat aatccttaaa accattcccg gataaaaaca attttgtcat
     2041 attttgggat gcttcttcca ttgaacgtaa gtaatcttca agctctttta gtgcatcagg
     2101 cagatgtatt tgactattta tttcggtggt gaagttaatt aagggaactg agaaaaacag
     2161 gcaaaaaata gcaagaatat aaataggaat ttttaccgat ttatttgctg atatatagct
     2221 aaaagggtgt ttatcaagta aagatgcaca aaccacagcg cctaaaatga aaacggatat
     2281 tgatgtaaat aattgaactt gctccatcca tgatttgtct gttgcgatta cgtttaaatc
     2341 agttccggat agtttagcaa ttaaaagaat gattgtgtac gaaaagcaaa ataagaagca
     2401 tggtactaaa ataattacca aactttttat aaaattattg cttttatcaa atataccgat
     2461 taatttcata tgttttgtgt aagtatagaa tctccgttgt cgcttacaga gagacttgtt
     2521 ttgtgtccta atattaatgg aagattataa tctgtatgtc cggcattaaa accataacaa
     2581 accgggaaag aataatcttt tactgcgttt tcaattatcc cgtatacacc atccgggaaa
     2641 cttgtatctt cttgaatatc agtaaactgt cctacaacta aaccggaaat ctgattaaaa
     2701 ataccaccga gtctaatatt ttgtatcatt cggtcaatgt gatatagagg ttcgcatacg
     2761 tcttctataa aaagtatgtt gtttttagct tgtaaatcaa atgctgttcc gcgaagtccg
     2821 tatataacag ataagttccc tccaactaaa ttacctaatg cagttccttt tttgttaaat
     2881 cgttcataac caattgaata acttggcaat tcaccaaaaa gcaattgttt tagacgtaat
     2941 gcaggctctt ccttagcttg tagtgctaaa tttttagtca ttatactatg tattgatgta
     3001 atgccatgca aagataatgc gctgtgaatg agtgtaatat cactaaatcc gagtatccat
     3061 ttcggtcttt cttgtaattt tgagaaatca atcttatcaa ttattctcgc taatccatag
     3121 ccgccgcgac tacacaaaat cgcatcacaa ttattatcat ctatagcaga ttgtatatca
     3181 gcgatgcgtt cttcgtctgt acctgcaaaa cgaccattcg cagcaaaaac cgattttcct
     3241 atagtaacaa taagtcccca tgactctaaa acgtcttttg cattccatac aaaatcagga
     3301 ttgacaacac ctgatggtga tagaatacag atatggctgc ctatatgtaa aaacggagct
     3361 tgaatcattg tttcttagct ttcatactta ttattgggat aatcatttcc tcaagtgaaa
     3421 tgccaccgtg ttggaatgtg tctttataat atgatacgta gtaattataa ttgtttggat
     3481 atgctataaa atcatcatta gatgagaaaa catatgtaga actaatgttt ggagaaggta
     3541 gtccgattag ttccggctta ttaattacta agaaatcttt cttatcgtag ttcaaattct
     3601 tgccgacttt atacctaaga ttgacattcg tatttttgtc gcctattact ttaaccggat
     3661 tttttactcg tatagcacca tgatctgttg taagtacaac cgaaacgcca ctttctgata
     3721 ggattttaaa gaattcagct attgacgaat gcttaaacca cgacaaaacc aaagaacgat
     3781 atgcagcttc atcaggaact agttctctaa acaattttac ctctgtacgt gcatgcgaaa
     3841 gcatatccac aaaattaaac actacaacat taagcggatt atcaagcaac tgtttaatgc
     3901 cttcgttaag tttttctccg gcctgattag tatttatttt gttatacgag aatttgtatt
     3961 tctttctaaa acggtctagt tgagtttgta ttaaggcttc ttcattcata tttttaccct
     4021 catcttcttc ttcgtcaacc cataattccg gatacatctt tcttatttgt gaaggcatca
     4081 atcctgagaa gattgcatta cgcgaatatt gagttgccgt tggtaagatg ctaaagaaag
     4141 tatcatcatc agtaaaatta aaccattttg ccaattcttt ttgcattacc cgccattggt
     4201 caagtctaag attatcaatt aatacaaaaa ataccttttc cccattatcc aatagaggga
     4261 atacctttgt tttaaatata tcgggactca ttaatgggcg cgcattgttt tccttgaacc
     4321 aattttcata attctttttt atgaatttta cgaatgttgc attggcttca tttttttgca
     4381 tttgcaacat ttcgtccata ttgttattgg cttgttctaa ttctaattcc cagaaaacca
     4441 agttcttgta tactttcttc caatcatcag tattgaatga atcatttatc tgcatactaa
     4501 tacgcataaa ctctgattgg taattggtag tggtcttttg tgaaataata tcctgagctt
     4561 gtacaacctt tttaattgcc ataagaatct ggttaggatt tacaggtttt ataaggtagt
     4621 cggatatttt ttgtccaata gcttgttcca tgatatcctc ctcttcgctt ttggttatca
     4681 taataacagg caattccgga tgttgttttt ttatttgtag tagagtttcc aatccgctta
     4741 ctcccggcat gttttcatcc aaaaacacta cctcaaagtt attctcgttg catgcatcaa
     4801 tagcatcttg accactattg acagcagtta cagaatatcc tttactttct aaaaaaatta
     4861 tgtgagggcg caacaaatct atttcgtcat cagcccaaag aatatgtatt tgtttcattg
     4921 ctgtcaaagt taactcaaat gcatctttga taatcttaat aaaaccttat atcatctaat
     4981 ttttatagat tagcagtagt gctttctcct tgctttttca tttttcctac aaaaatagaa
     5041 cctaccatca ttggtatcac ataattaatg aaccatactg acatacctgc aagtgctgct
     5101 ccgacagtat ttgacgcaaa tgctccgata aataacactg cgtatgagcc tcgcactcct
     5161 aattcggaaa atgctattgt tggagttaca gataccatca aataatttaa tggaagtatc
     5221 attgcagcat cagttagtga tagttctaca tcaaagaaac gcagtaagaa ataatattgc
     5281 gaacaataca caatgtatct aattagggat aaaaatgtaa catagaacaa actgcgtggg
     5341 tttatgtatg atatggcttc taataagctc cttagtcttt taaatctgtt gcgttggaat
     5401 aatatcttcc cccaaatagg taaggtaaaa tagagagaga taaaagctat tataagcagt
     5461 atagatgcga tctgtatatt taaggaatcc tttatagatt caaatggcga aaaaacgatt
     5521 agcaatatac cggctgttaa tccgcaaacc agagtagaga atgtttgtcc aagaacccca
     5581 ataacagaaa gtacaatccc ttttgtgcga ttgcatgcat ttaatataag aactcttcct
     5641 aaaggatctc ctattcggtt aggcgtaaag aaaccaactg ttaagcccat taaaaccgat
     5701 ttatatgctt tacacaatga aaccggttga agcttttgtg atagatgttg ccatttcatt
     5761 gattctaaca accaattaca tggtattaaa agcactgcaa gcacaagtag tatggtacga
     5821 aatgcagaaa tgtttttgaa actattaaaa aactcattat atcctttaaa gtttacaagt
     5881 acataagcca gataagcgta tgcacaaacc ataataagta ttttaataaa tttataatac
     5941 ctatcaatca agctgtgtcc tttttctagt aattcatcca ttttatt
//
LOCUS       DAOPID010000081         5965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_17462_length_5965_cov_4.551946, whole genome shotgun sequence.
ACCESSION   DAOPID010000081 DAOPID010000000
VERSION     DAOPID010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 5965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5965
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17462_length_5965_cov_4.551946"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..150)
                     /locus_tag="PK641_03900"
     CDS             complement(<1..150)
                     /locus_tag="PK641_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HPG55528.1"
                     /translation="MPDTGKQFDAAIAQCKAVYVKKMDDYGPAWRILRPESVTDQIFI
                     KANRIR"
     gene            complement(187..699)
                     /locus_tag="PK641_03905"
     CDS             complement(187..699)
                     /locus_tag="PK641_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HPG55529.1"
                     /translation="MALIKSVRGFEPKIGNDCFLAENATIVGDVEIGEGCSIWFNVVL
                     RGDVNSIRIGNHVNIQDGSVLHTLYQKSTIEIGDYVSIGHNVTIHGAQVKDYALIGMG
                     ATVLDQAVVGEGAIVAAGALVLSNTIIEPNTIWAGVPAKFVKKVDPEQSKEINQKIAH
                     NYAMYASWYK"
     gene            complement(729..2141)
                     /locus_tag="PK641_03910"
     CDS             complement(729..2141)
                     /locus_tag="PK641_03910"
                     /inference="COORDINATES: protein motif:HMM:NF028437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HPG55530.1"
                     /translation="MKFKFLFAAILSGIMFTVNAKENLNIQVKSTDLAGQQVFLGQYY
                     MSKLYTKDSIMLDKNGRGTFVSKEKYPEGLYVMYFSDSKYFDVLLSDKQQFTIKIDTT
                     DIPKNIVFEGAEETKAFHDYSQFLLQKQINGKALNERKKQSKDSSEIKSIEAELDLLN
                     IEVKTRQNDLMAQYKGGMLELFLKELVVPEFKETELPAGSKNPDSIKAINRYVFYKQH
                     YFDNVDFSDERTYRTPYIGNTLQLYLDKVLVQSYDSIIPPAINLIERSKGSEKAFQSM
                     CSFMLDYSLKSKIMGMDSLMVTIGRKYYLSGEAKWADSTLLSNLAKEIKKIDKSLVGM
                     KAKNIALCDTLGQYHSIYDMGGSQLTILFFYEPSCGHCKKTSPLLRDFYDKYKDDPRI
                     KVIAVYMLTDQEEWMKFLKEIKMENMTNVWDPHRTSYYWQWYDTSSTPMIYVLDKDYK
                     VFAKKIDVETLEMIAKYELK"
     gene            complement(2131..3894)
                     /locus_tag="PK641_03915"
     CDS             complement(2131..3894)
                     /locus_tag="PK641_03915"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HPG55531.1"
                     /translation="MSETVGHLYELRCMMKEYGVSACLIPQADPHQSEYLEDHYKVRE
                     YFSGFTGSAGLLAVSLTDVGLWTDSRYYLQASEQLQLSGIKLYKQGLTDTISYDKWLY
                     NQVGDCGVVVVNANNISVNEWIRLSKIVKLKDSDSFESLWFNRPPLSDSKIFIHDVKY
                     SGKSVDKKLTEFRKAILDAKADCCILTVLDEIAWLLNIRAFDVPHNPVVRSYLIVNKD
                     ECILFVDLNKLSPELVKYFAINNISVREYDEVGVFISNMQNVRVLYDADKMSFRLYSK
                     FSSTIIAICKTSFVSAMKAVKNDTEIRGFKNAMKRDGVVWVKFLRWFELNQQNGIKMT
                     ERSVMNELRKLKSEQNGFVEESFETTVGYGKHAAIVHYAATEQSDAVIKNSGLLLIDT
                     GTQYLDGTTDTTRTLACGSLTQQEKIDYTLVLKGHIALAQAIFPLGTKGSQIDILARQ
                     YLWQKGLDYGHGTGHGVGHFLNVHEGYARISPRENGPIIEAGFTFTNEPGLYREGCHG
                     IRIENMLLVVDDKKTENTKFLRFETLTLVPIDMKAIDLSLLDENERNYLNNYHKRVIT
                     LLSPLLNEEENNWLKNTLYEI"
     gene            4043..4234
                     /gene="rpsU"
                     /locus_tag="PK641_03920"
     CDS             4043..4234
                     /gene="rpsU"
                     /locus_tag="PK641_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HPG55532.1"
                     /translation="MIVVPVKEGENIERALKKFKRKFEKTGVVKELRKRQAFSKPSEV
                     SRERMIHAIYVQQLQQGEE"
     gene            4293..5183
                     /locus_tag="PK641_03925"
     CDS             4293..5183
                     /locus_tag="PK641_03925"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPG55533.1"
                     /translation="MISAFLQYLQYEKNFSSHTVFAYKKDLEQFSLFVSDKYNTQNLT
                     EVKSDYVRDWIISLKDTKLTSTSINRKISALRSFYKFCRKHNSNIGDPLAKVSAQKTG
                     KKLPYFFKDSEIESVLSDNPIVDNFSDSRDDIILETLYDTGIRRAELITIKDEDFNFF
                     SLTLRVMGKGNKERLIPISNILKVKAENYINLKKSLFGNTDSFIVTDKGCSTYPNFIY
                     RVVKDKMSTVSMLDKRSPHVIRHTFASGMLNNGAELNAVKDLLGHANLSATQIYTHTS
                     FEQLRSIYKQAHPRNNKNNH"
     gene            5203..5496
                     /gene="raiA"
                     /locus_tag="PK641_03930"
     CDS             5203..5496
                     /gene="raiA"
                     /locus_tag="PK641_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HPG55534.1"
                     /translation="MELNIQAIKFDATAKLQSFILKRMEKLDRFYEGISTIDVTLKVV
                     KPETSVNKTATIKLSAPHLELFAEKTADSFEQAIDECAEALQKQLIKAKEKKQ"
     gene            5666..5739
                     /locus_tag="PK641_03935"
     tRNA            5666..5739
                     /locus_tag="PK641_03935"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5700..5702,aa:Thr,seq:tgt)
     gene            5770..5851
                     /locus_tag="PK641_03940"
     tRNA            5770..5851
                     /locus_tag="PK641_03940"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5804..5806,aa:Tyr,seq:gta)
     gene            5890..5962
                     /locus_tag="PK641_03945"
     tRNA            5890..5962
                     /locus_tag="PK641_03945"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5923..5925,aa:Gly,seq:tcc)
BASE COUNT         1995 a         1166 c          855 g         1949 t
ORIGIN      
        1 tctaatgcga tttgctttta taaatatttg atcagttact gattccgggc gcagtatacg
       61 ccaagcagga ccatagtcat ccatcttttt tacgtaaaca gccttacatt gcgcaatggc
      121 agcatcaaat tgtttccctg tatcaggcat attttgtatt tttaatagac cacaaagtta
      181 ataattttat ttataccaac ttgcatacat ggcatagtta tgtgcaatct tttggttgat
      241 ctcttttgac tgctcagggt caactttttt tacaaattta gcaggaaccc ccgcccaaat
      301 ggtattaggt tcgataattg tattggatag aactaatgct cccgccgcta caatagcacc
      361 ttcacccaca acggcttgat ctaaaactgt agcacccatt ccaattaaag catagtcttt
      421 aacttgcgca ccatgaatag taacattatg tcctattgaa acataatcac ctatttctat
      481 tgttgatttc tggtacaaag tatgtaatac tgaaccatct tgtatattaa catgatttcc
      541 tatacgaata ctatttacat ctcctctcaa cacgacatta aaccaaatgc tgcatccttc
      601 gccaatttct acatcgccta caattgttgc gttttcggct aaaaaacagt cattgcctat
      661 ttttggttca aatcccctaa cagatttaat aagtgccatc ttttgtttat ttaatattat
      721 atgaatactt attttagctc gtatttagca atcatctcta aagtctctac atcaattttc
      781 tttgcaaaaa ccttataatc tttatctaaa acataaatca taggtgttga agatgtatcg
      841 taccattgcc aataataaga tgttctatga ggatcccaca catttgtcat attctccatt
      901 ttaatctctt tcaaaaactt catccattct tcctggtctg ttagcatata tacggcaata
      961 actttaatac gaggatcatc tttgtatttg tcgtaaaaat ctctcaataa aggacttgtc
     1021 tttttacaat gtccgcacga tggttcgtaa aaaaataaaa ttgtaagttg agatccaccc
     1081 atatcgtata tagaatgata ttgaccaagt gtatcgcaca aagctatgtt ctttgccttc
     1141 atgccgacca atgatttgtc tatcttttta atttcttttg caagatttga aagtaaagta
     1201 gaatcagccc attttgcctc gcccgacaaa taatacttcc ttcctattgt aaccattagg
     1261 ctgtccatac ccataatttt acttttcaag gagtaatcta acataaaact acacattgac
     1321 tgaaatgcct tctccgaacc cttactacgt tctattagat ttattgcagg tggtattatc
     1381 gaatcgtacg attgcaccaa caccttatct agatacaatt gcagagtgtt tccgatatat
     1441 ggcgttctat atgttctctc atcagagaaa tctacgttat caaaataatg ttgtttataa
     1501 aacacatagc gatttatggc ctttatcgaa tccggatttt tactgcctgc aggcaattct
     1561 gtttctttaa attcaggaac gactaattct tttaggaaca attctaacat tccgccctta
     1621 tattgagcca tcaaatcatt ttgtcttgtt tttacttcaa tattcagtaa atctaattct
     1681 gcttcaatcg acttaatttc ggatgagtct ttagattgtt ttttgcgttc attcaatgcc
     1741 tttccgttta tttgcttttg tagcaaaaac tgagaataat catgaaaagc ttttgtctcc
     1801 tcagctcctt caaacactat attctttggt atatctgtag tatcaatctt aatagtaaac
     1861 tgctgtttat cagacaaaag aacatcaaaa tacttagaat ctgagaaata cattacatat
     1921 aaaccctccg gatatttttc tttgcttaca aaagttcctc tgccattctt atctagcatt
     1981 attgaatcct tggtgtacaa cttactcata tagtattgac ccaaaaagac ttgctgcccg
     2041 gctaaatcag tagattttac ttgaatatta agattttctt tggcattaac agtaaacata
     2101 atgcctgaca aaatagctgc aaacaaaaat ttaaatttca tataaagtat tttttaacca
     2161 attattttct tcttcattta acaatggtga taataatgta atcacccttt tatgataatt
     2221 attcaggtaa tttctttcat tttcatcaag taaactcaaa tcaattgctt tcatatcaat
     2281 cgggaccaaa gttaatgttt caaaacgtaa aaacttagta ttttcagttt tcttatcatc
     2341 tacaactaaa agcatatttt caatacgaat gccatgacat ccctcacgat acaaaccagg
     2401 ttcattagta aaagtaaatc cggcttcaat aatcggacca ttttcacgtg ggctgattct
     2461 agcatatcct tcatgtacat ttaaaaaatg tcctacacca tgacccgttc cgtgcccata
     2521 atcaagtcct ttttgccata aatattgacg agccaaaata tctatctgcg aacccttagt
     2581 acctaatgga aatatagcct gcgccaatgc tatatggccc tttaaaacca atgtataatc
     2641 aatcttttct tgctgagtca atgaaccgca agccaaagtt ctagttgtat ctgttgttcc
     2701 atccaaatat tgagtacctg tatcaatcaa caatagacct gaatttttaa tcacagcatc
     2761 actttgttct gtagctgcat aatgcacaat tgcagcatgt ttaccatatc cgactgttgt
     2821 ttcaaaactc tcttcaacaa aaccattttg ctccgatttt agctttctta gttcgttcat
     2881 aaccgaacgt tctgtcattt ttatgccatt ttgttgattt agttcaaacc atcgtaagaa
     2941 ctttacccaa actactccat ccctcttcat agcgttcttg aaaccgcgta tttcagtatc
     3001 atttttaact gctttcattg ctgatacgaa agaggtctta caaatagcga ttatcgtaga
     3061 tgaaaattta gaatacaatc taaatgacat cttatctgca tcatacaaaa ctctaacatt
     3121 ttgcatatta cttataaaca cacccacttc atcatattca cgtactgaaa tattatttat
     3181 agcgaagtat ttgactagtt cagggctaag tttgtttaaa tccacaaata aaatacattc
     3241 atctttgttt acaataagat atgatcttac taccggatta tgcggaacat caaaagctct
     3301 aatatttaac agccaagcga tttcgtccaa aacagtaaga atgcagcaat ctgctttcgc
     3361 atccaaaatt gctttcctaa attcagtaag ttttttatct acgctctttc ctgaatattt
     3421 tacatcatgt ataaagattt tactatcact aagtggaggt ctattaaacc ataaactttc
     3481 aaaactatcg ctgtctttta gttttactat tttgcttagt ctaatccatt catttacaga
     3541 tatattattt gcatttacaa ctacaacacc acaatctcct acttgattgt ataaccattt
     3601 atcatacgat attgtatctg tcagaccttg tttatataat ttaattcctg acaattgcaa
     3661 ttgttctgat gcctgcaaat aatatctaga atccgtccac aaaccgacat cagtcaaaga
     3721 aactgctaat aaacctgcag agccggtaaa gccactgaaa tattcacgaa ccttataatg
     3781 atcttctaaa tactcacttt gatgaggatc tgcttgggga atcagacagg ctgacactcc
     3841 atattctttc atcatgcaac gtaattcata caaatgtccg actgtttcac tcatttcaaa
     3901 cgtacataga attatataat gtgcaaagtt atagttttct gcatcttttt tcaaaaaaaa
     3961 tgaaagaaaa gtttgttaat aaaaaagctt tacgtaactt tgccccctca aaaatccgac
     4021 tataaattta atttattaat atatgatcgt agtacctgta aaagaaggcg agaacattga
     4081 aagagccttg aagaaattta aacgcaaatt cgaaaaaacc ggtgttgtta aagaattacg
     4141 taaacgtcag gcatttagca aaccatctga agtatcccgc gaacgcatga ttcatgcaat
     4201 ctacgtacaa caactacaac aaggcgaaga ataatttttt attctgtttc tgattcgcta
     4261 tatttgcaat ataatagcaa atagcagcga acatgattag tgctttttta caatacttgc
     4321 agtatgagaa gaatttttct tcccatactg tttttgcata taaaaaagac ctcgaacaat
     4381 tctcattgtt cgttagtgac aaatacaaca cacaaaacct taccgaagtt aaatctgatt
     4441 atgttagaga ttggattatt tcactaaaag ataccaagtt aacatccaca tccatcaaca
     4501 gaaagatttc tgcgctccgt tcattctata aattttgcag aaaacacaat tcaaatattg
     4561 gtgatccact agcaaaagtt agcgcacaaa aaacaggtaa aaaacttcca tattttttta
     4621 aagactccga aatagaaagc gtactctctg ataatccgat tgttgacaat ttttccgatt
     4681 caagagatga tattattttg gaaactttgt atgacaccgg tattcgtaga gccgaattaa
     4741 taacaataaa agatgaagac tttaattttt tttcgctaac tttgcgtgta atgggtaaag
     4801 ggaataaaga gcgactaata cctattagta acatattaaa agtaaaggca gaaaattaca
     4861 ttaatttaaa gaagagtcta tttgggaata cagattcatt tattgtgaca gataaaggat
     4921 gctcaactta tcccaacttt atatacagag tagttaaaga caaaatgagt actgtaagta
     4981 tgcttgacaa gcgaagtcca cacgtaataa gacatacatt tgccagtggt atgctgaaca
     5041 acggagccga actaaatgcc gtgaaagatt tattaggaca tgcaaattta tctgcaacac
     5101 aaatatacac acacacatca tttgaacaat tgagatcaat atacaaacaa gcacacccta
     5161 gaaacaataa aaataaccat taattaaaga aagggaaaaa ttatggaatt aaacattcaa
     5221 gcaattaaat ttgatgctac agcaaaactt caatcattca ttctgaaacg catggaaaaa
     5281 ttagatcgtt tctacgaagg aataagcaca atagatgtca ctcttaaagt agttaagccg
     5341 gaaacatcag taaataaaac agccacaatt aaattatctg caccacattt agaactattt
     5401 gcagaaaaaa cagcagattc cttcgaacag gctatagatg aatgcgccga agcactacaa
     5461 aaacaactaa tcaaagcaaa agaaaagaaa caatagagta ttaaaaccac aaatacctag
     5521 taataacaca tatagctaca gtttcagact tttgactata aaaaagtgat ttttttattc
     5581 aattattttg cagatataaa aaaaacacct acatttgcaa gccgttttaa caaacctatg
     5641 ttagaacggc tttttagcca agaatgcctc tttagctcag ctggccagag cacgtgattt
     5701 gtaatctcgg ggtcgttggt tcgaatccga caagaggctc aaaaaaaaat aggttttgtc
     5761 ctatgtaatg ggcagttacc agagtggcca aatggggctg actgtaactc agctgtttta
     5821 aacttcggtg gttcgaatcc atcactgccc actgaattgc aggataattt gcaagattga
     5881 caaaaaaatg cgggaatagc tcagttgata gagcattagc cttccaagct gagggtcgcg
     5941 ggtttgagtc ccgtttcccg ctctt
//
LOCUS       DAOPID010000082         5953 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_17516_length_5953_cov_3.636148, whole genome shotgun sequence.
ACCESSION   DAOPID010000082 DAOPID010000000
VERSION     DAOPID010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 5953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5953
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17516_length_5953_cov_3.636148"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="PK641_03950"
     CDS             <1..652
                     /locus_tag="PK641_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55535.1"
                     /translation="LWRKYHVKRHHNLFLKRAIGGRFEGANKANFSDAVLLHEIKNLP
                     PMKMTYVDIVNPSGFQYFRYLSPDSSYCSIAEIKLYDGTQHLIYGNIIGTNGHYVQSP
                     NCVKENVFDGDVLSYFDAPNPNEAWVGMQFSDKRCIKKIGYIMRNDDNFVQEGQCYEL
                     LYMDKNGWKSMGKKTAIADSIVYQNVPSGAAYLLKNHTKGKEERIFTYENGQQIWW"
     gene            659..1864
                     /locus_tag="PK641_03955"
     CDS             659..1864
                     /locus_tag="PK641_03955"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG55536.1"
                     /translation="MDFWKEILTYLLRNKGRTLTCGIGISWGIFMLCILVGVGNGLEN
                     GILNLFDAYSKNATCVYGGEISVAEQGNFIGQQIRFSDTDLSLLKKSVSNIQYISPEC
                     TQLVSMHTNNNIDNFELKGIDIDYLQIKNLRASQGRILNPWDFMQNKNHIIIGQHIAE
                     VLFPKQTAISKYIHINDEVFKVVGIIKNSSTIALESQCAYIPLAAYKRTISNQQVFQV
                     FWYTDQNNNCEKQVKIYLQKYYHINRNDDKALYIQTMEEQTQSFKSLFGGIRGFLWFI
                     GISTLIGGIIGVGNIMHASVKERMRDIGIRKAIGAKSSDIKKMVISESLVITLLAGIS
                     GSVIGWMCLRIIGFYLSEESILKDPQIDAATIAVSVVALMLSGLIAGLKPAIYAAALR
                     PIESLKIEN"
     gene            1865..2923
                     /locus_tag="PK641_03960"
     CDS             1865..2923
                     /locus_tag="PK641_03960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPG55537.1"
                     /translation="MNAFKYIIIGTFILFTAGIISIAILQQNKDLPYTTARFRDINKE
                     LVISGQIDPLKEIEIKSPISGILEHLYVQIGDTITTGQAIARVQFVRNPVELKQLLKE
                     LEVSKTRYQTQQKQFERSQSLYQKGVISSEDYEKEQSTFAIYKQEYLALLSEYDMLQG
                     KYEEKGISNIIQATGSGTILDLPIKEGGSVMARGALSEGTSVVRIADLKSLVFSSDVT
                     EAHILKLHIGDSLHISVATDPTQKIIGIISLISPVASLREGQRRFSIKADIKITHENA
                     PFLRAGSSATAHVILEGEKQVLSIEESMLIFENDSCYVEIIDKKDHIQKQAIHTGISD
                     GVYIEIKSGIDSTSHIKL"
     gene            2920..4131
                     /locus_tag="PK641_03965"
     CDS             2920..4131
                     /locus_tag="PK641_03965"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPG55538.1"
                     /translation="MNNIQRQKYIVTIIAILFLFCGIFSLRACSVFIPLFIWAGYKSD
                     KFGIRQPVAKRWIIGVFVIFVLIGINILLSYYQSNSVSLGLSIALLLLFDIFVFDNIS
                     RQYIIWIYRFICLLSTCVIIISLTAYTLFHCEIIQLKLMPLCDMKFLYKPFGCPINEW
                     GTLILVLIPFNLYSYFIEKSKLNYLMLINIALLSSCIALSYCRSLYGSLFLLYLSVLI
                     YVVIGKITYRQSWAKLIFLALAGFLAPILIPNNAVITTLKANETVSQQRSIDARIDRL
                     DNVINYADEHRLYGYGLGNYLLSTQSNKSEYETLVSPAAPNTFIQIYIETGYIGFGIF
                     TILLLGLLSYLFYHIKAGENIPALLCSGTILAFLFRDMAYATLLHVHYVLILSNLLIV
                     LLLIDINRTVR"
     gene            4128..5183
                     /locus_tag="PK641_03970"
     CDS             4128..5183
                     /locus_tag="PK641_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55539.1"
                     /translation="MKQTLYILCIIIASSLAVVDFLFVLWGKNNSITNNPVSYINQAC
                     KITECDSSNIRLENLLKNSFVYSLKTDSATSILSKAYVLNPYCEQIIYAYAICLYLNK
                     ESVTAAHVLQSKSILRYKETLWLDGFIAEQKHDTLECINKYAKLIYAYPDVLDSQFFD
                     DLYQRNPYLALKAVSVAFCIVQKEWNKEHNPLIAAKIGKFNLYKGQIEKASKCFTYCM
                     QQLPNLNRPYLYQGIIYLSQKDSVNAQLCFEQANVLDPADHLPNYYLDIMEHKTFSGN
                     KYHVFYRTPVELETCYHSRLVSNSLIIPDLLAYLSPDITLTEAYKQFYKNGILQNSLY
                     SKGGIENPIKRVEKNTN"
     gene            5195..5392
                     /locus_tag="PK641_03975"
     CDS             5195..5392
                     /locus_tag="PK641_03975"
                     /inference="COORDINATES: protein motif:HMM:NF021923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HPG55540.1"
                     /translation="MKNLSEKEMSEVIGGSRCNAGCNLAGSVLIGISIGAAGLSFGAS
                     LAAGAFGAFLWDQGVCGSICG"
BASE COUNT         1987 a          972 c         1038 g         1956 t
ORIGIN      
        1 cttatggcga aaatatcatg taaagagaca tcataattta tttttaaaac gcgcaatcgg
       61 cggccgattt gaaggagcaa ataaggctaa ttttagtgat gcggttttat tacacgaaat
      121 taaaaattta cctcctatga aaatgacata tgttgacatt gtgaatccat cgggttttca
      181 atattttcga tatttatcgc cggatagttc ttattgcagt atagcagaaa ttaaattata
      241 cgatggcacg caacatttga tatatggaaa tattatcggg acaaacggac attatgtaca
      301 aagtcctaac tgtgtcaaag agaatgtttt tgatggcgat gtccttagtt attttgatgc
      361 tcctaatccc aatgaagcat gggttggaat gcagtttagt gataaaagat gtatcaaaaa
      421 aattggatat atcatgcgaa acgatgataa tttcgttcaa gaggggcaat gctacgaatt
      481 actatatatg gataaaaacg gatggaaaag tatgggcaaa aaaacagcca ttgctgattc
      541 aattgtttat caaaatgttc catccggagc tgcttactta ttaaagaatc acacaaaagg
      601 taaggaggaa cgaatattta cttatgaaaa tgggcaacaa atttggtggt aaagaataat
      661 ggatttttgg aaagaaatac taacatatct acttagaaat aaaggaagaa cattgacttg
      721 cggtattggc attagctggg gtatctttat gttatgcata ttagttggcg taggtaatgg
      781 tttagaaaat ggcatcctta atttgtttga tgcctatagt aaaaacgcaa cctgcgttta
      841 cggaggagaa atttctgtcg ctgagcaagg taattttatc ggacagcaaa ttcgattctc
      901 agataccgat ttgtcgctat taaagaaatc cgtatccaac atccaataca tttcaccgga
      961 atgtacacaa ttagttagta tgcatacgaa taacaatatc gataattttg agttaaaagg
     1021 aatagatatt gattatttac aaataaaaaa tttacgggca agccaaggca gaattctaaa
     1081 tccttgggat tttatgcaaa acaagaacca tattattatt gggcaacaca tagctgaagt
     1141 gttatttcca aagcaaacgg caatcagcaa atacatacac ataaacgatg aggtatttaa
     1201 ggtagttggt attatcaaaa attcctcgac gattgcatta gaaagccaat gtgcttatat
     1261 tccattagcc gcatataaaa gaacgatatc aaatcaacaa gtatttcaag tcttttggta
     1321 caccgatcaa aataataatt gcgaaaaaca agttaaaatt tacctgcaaa aatattatca
     1381 tattaatcgc aatgatgata aagcattata catacaaact atggaagagc aaacgcaaag
     1441 ttttaaaagt ttgttcggtg ggattcgcgg ctttttatgg tttatcggaa tcagtacact
     1501 tattggtggt ataatcggtg tcggaaatat tatgcatgcc tctgtaaagg agcggatgcg
     1561 agatattgga attcgcaaag caataggagc aaaatcatcc gatattaaaa aaatggtcat
     1621 atcagaatcg ctcgtaatta cgctgcttgc aggtatatca gggagtgtca tcggttggat
     1681 gtgtttacga ataattggtt tttatttatc agaagaatcc attcttaaag atcctcaaat
     1741 tgatgctgca acaattgctg tttcggttgt tgctttaatg ctatctggtt taatcgctgg
     1801 actaaaaccg gcaatttatg cagcagcttt acgtcccatt gaatcattaa agattgaaaa
     1861 ttaaatgaat gccttcaaat atattatcat aggtactttt attcttttca ctgctggaat
     1921 tattagcatt gctatattgc agcaaaataa agacttacca tatacgactg ctcgatttcg
     1981 tgacataaac aaagaattag taatcagcgg acaaattgat ccactaaaag aaattgaaat
     2041 taaatcgccg atatctggta tcttagaaca tttgtatgta caaataggtg atactataac
     2101 aactgggcaa gctattgctc gtgttcaatt tgttcgcaat cctgttgaac taaaacaatt
     2161 attaaaagaa ttggaagtat ccaaaacaag atatcaaacg caacaaaaac aatttgaacg
     2221 ttctcagtct ctttatcaaa aaggggttat ctcttccgaa gactacgaaa aagagcaaag
     2281 tacttttgct atctataaac aagaatacct tgcactttta tcggaatatg acatgttgca
     2341 aggaaaatat gaagaaaaag gaatctcaaa tatcattcaa gcaactggat ctggcactat
     2401 tttagatctt cctattaaag aggggggatc tgttatggca agaggtgccc tcagcgaagg
     2461 tactagtgta gtacgcattg cagatctaaa atctttggtc ttttcatcag atgtaactga
     2521 agcacatatt ttgaaattac atattggcga cagcctacat atttcagttg ctaccgatcc
     2581 cacacagaaa atcataggta ttatttcact aatatctcct gttgcatccc ttcgtgaagg
     2641 acaaagacgc ttttctatta aagcagatat taaaatcacg catgagaatg ctccttttct
     2701 gcgagctgga agttctgcaa cggcacatgt gatattagaa ggggaaaagc aggtgcttag
     2761 catagaggaa agtatgctaa tctttgagaa tgactcttgc tatgtggaaa taattgacaa
     2821 aaaagaccac attcaaaaac aagctataca cacaggaata agtgatggtg tatatattga
     2881 aataaaaagt ggcattgaca gcacaagcca tattaaatta tgaacaacat tcaacgacag
     2941 aaatatattg taactataat agcaatacta ttcttgtttt gcggcatatt ttctttacgc
     3001 gcatgtagcg tttttattcc tctatttatt tgggcaggtt acaaatccga taaatttgga
     3061 atacgccagc cagtggctaa aagatggatt ataggagttt ttgtaatatt cgtcttaatt
     3121 ggcataaaca tcttattaag ttactatcaa tctaattctg tttcattagg tttaagtatc
     3181 gctttgcttt tattattcga tatattcgta tttgataata tatcacggca atatattata
     3241 tggatatacc gtttcatttg tctgcttagt acgtgtgtaa ttatcatcag cttgactgca
     3301 tataccttat ttcattgcga aataattcaa ctaaagctga tgccactatg cgatatgaaa
     3361 tttttgtaca aaccatttgg ttgtcctata aatgaatggg gtacattaat tttagtacta
     3421 atacccttta atctatattc ctactttatc gagaaaagta aattaaacta tttaatgctt
     3481 atcaatattg ccttgctaag ttcttgtata gcattaagtt attgtcgctc tttatatggt
     3541 tcactttttt tactttatct ttctgtttta atatacgttg ttataggcaa aataacatac
     3601 cgccaatcat gggcaaaact tatttttctt gcattggctg gttttcttgc gcccatacta
     3661 attcctaaca atgcagtaat aactacctta aaagcgaacg agactgtttc tcaacagcgt
     3721 agtattgatg cacgtattga taggctggat aacgttatta attacgcaga cgaacatcgt
     3781 ttgtatggtt atggcttagg aaattatcta ttatctacac aatccaataa atccgaatat
     3841 gaaacacttg tatcaccggc tgctcccaat acgtttattc aaatttatat agaaaccgga
     3901 tatatcggct ttggaatttt tacgattcta ttattgggat tattaagtta tctcttttat
     3961 cacattaagg ccggagaaaa cattccggct ctattatgct ccggaaccat tttagccttt
     4021 ctgttccgcg atatggctta tgccacgttg ctacatgtac attatgtatt gattctaagt
     4081 aatcttctta ttgtattgct actaatagat ataaatagaa ccgttcgatg aaacagactt
     4141 tgtacatatt atgtatcatc atcgcatctt ctttggctgt ggttgacttt ttattcgttt
     4201 tatggggaaa gaataattct atcaccaata accctgtttc atacatcaat caggcatgca
     4261 aaatcacaga atgcgatagt agcaatattc gtttggaaaa tttgttaaag aattcatttg
     4321 tttactcatt aaaaacagat agtgcaacat ctattctttc caaagcttat gttctaaatc
     4381 catattgtga gcaaattatt tatgcctacg ctatttgttt gtatttgaac aaagaatccg
     4441 ttactgcagc ccatgtactt caatcaaaat cgattctccg ctacaaagaa acgctatggt
     4501 tagatggatt tattgcagag caaaagcatg ataccttaga atgtataaat aaatacgcga
     4561 aattaatata tgcttatcct gatgtattag atagccaatt ttttgatgat ttatatcaac
     4621 gcaatcctta tttggcttta aaagctgttt ctgtggcttt ttgcatagta caaaaagaat
     4681 ggaacaaaga gcacaatcct ttgattgctg ctaaaatagg caaattcaat ttatacaaag
     4741 gtcagataga aaaggcttca aagtgtttta cttattgcat gcaacaactt ccgaatttga
     4801 atcgtcccta tttgtatcaa ggtattattt atctttctca aaaagacagt gtgaatgctc
     4861 aactttgttt cgaacaagcc aatgtacttg accctgccga tcatttaccc aattactacc
     4921 ttgacattat ggaacacaag acattttcag gcaataaata ccacgtcttt tatcgcactc
     4981 cggttgaact tgaaacttgc taccatagcc gattggtgag caactcatta attataccag
     5041 atctacttgc gtatttatca ccggatatta cgctaaccga agcgtacaaa caattttata
     5101 aaaatggaat tttgcaaaat tcgctatata gcaaaggagg aatcgaaaat cctatcaaaa
     5161 gagtagaaaa aaatacaaat taaaattagt ctttatgaaa aatttatcag aaaaagaaat
     5221 gagtgaagta attggaggta gtcgatgtaa tgcgggatgc aatcttgcgg gtagcgtatt
     5281 gataggaata agcattggag ccgctggatt atcatttggt gcaagcttag ctgcaggagc
     5341 ttttggggct tttctgtggg accaaggagt atgtggaagt atctgtggat aataatttaa
     5401 tataaatttt tatcagacag aaaacatagt ttcagattcc gtgtactagg cggaatctga
     5461 aactatacaa aattaattta tcccattttt tatacaaaca attatcaagt aatgtacaca
     5521 actctattat atctcatcaa acctgaatta aaccgatctg caatgcgaaa tacctttata
     5581 gctaatgtat tagccatttt tctttctttt ggaatattaa tatggggaaa tattccaaat
     5641 ctagagctaa acttttttat ttctattatg ccttattggt gtatctgtag tctgttttta
     5701 gagcttggct gcaatccaga attgccattt gcggatttta tattatcagt gaagcccaaa
     5761 ctatttcaga aaattatgat ataccgttac gttctttcat gcattatatc attaataatc
     5821 cttttgtgtt atatgatgtt attcccctca ggaataatac tcaaacactt atcagctttc
     5881 tgcataggtt tttgttttcc ttctttttgc tacattttat tatatccaca ggccaataag
     5941 tcgattaata tta
//
LOCUS       DAOPID010000083         5910 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_17690_length_5910_cov_4.582408, whole genome shotgun sequence.
ACCESSION   DAOPID010000083 DAOPID010000000
VERSION     DAOPID010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 5910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5910
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17690_length_5910_cov_4.582408"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="PK641_03980"
     CDS             <1..979
                     /locus_tag="PK641_03980"
                     /inference="COORDINATES: protein motif:HMM:NF017964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HPG55541.1"
                     /translation="DRFESIVKTAIREINKLMNGENSEFIEQIDAPDALLWFIWSIQQ
                     YGTHESKEDMFRKYGDICNEIMQFIISQKHPNLYLHDNGLLYVDGRDTPMTWMNATEN
                     NKPITPRTGYVVETNALWYNALCFISEYANRLKDTKAQKAYESLAEKVKKTFIQTFWN
                     GIYLYDFVNGSEHDLEVRPNMIFAVSLPFSPLDKYQKKSVLDICTRELLTSKGLRSLS
                     PKSGCYRPNYIGGMQERDRNYHNGPVWPWTTGAYAEAYLIVHKQSGISFVERMMIGME
                     AEMKKLCIGTLSELYDGNPPYKGHGAMSFAPTVAEIIRTLTLLKQYNEA"
     gene            1010..2266
                     /locus_tag="PK641_03985"
     CDS             1010..2266
                     /locus_tag="PK641_03985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPG55542.1"
                     /translation="MKALMFGWEFPPHILGGLGTASYGLTRGMSVQDDLEITFVIPKP
                     YGDEDQSFLKLIGACNVPVVRKENSWDHVNERIGRIMHPNEYFWYRDHIYYDFTRIGT
                     NELGCIEFSGRYPDNLIQEIDNYEAVAGVLAHSLEFDVIHSHDWLTYPAGIFAKQISG
                     KPLVIHVHATDFDRSRGNVNPTVYNIEKRGMDIADHIICVSNLTRNTVIEKYHQHPDK
                     VTTVHNAVDPVPNVEHFKKEERKDKLVTFLGRITMQKGPEYFVEAAYRVLQRSKNVRF
                     VMAGNGDMMQAMVDLAASRGIADRFHFPGFLKGSEVHQMLANSDVYVMPSVSEPFGIS
                     PLEAMQVGTPSIISKQSGCAEILAHAIKTDYWDIDAMADAIYAIVEYSSIYKTLSEEG
                     IKEVNSITWDKAGLKVRSIYNRVTGR"
     gene            2285..3562
                     /locus_tag="PK641_03990"
     CDS             2285..3562
                     /locus_tag="PK641_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HPG55543.1"
                     /translation="MKNICFYFQVHVPYALRRYRFFEIGKDHYYYDDFGTEQQIRELA
                     DKSYLPANRIIANLIRTSGGKFCCGFSISGAAIEQLEQYAPEVIDSFKELADTNCVEF
                     LAEPYAHSLSSVFNDNEFELQVKKHSAKIKELFGKKPTSFRNSELIYSDEIGEKVAKL
                     GYNTILLEGAKHILGWKSPNYVYGHPYLPKVKLLTRNFSMSNHIPFEFSNTSWADYPL
                     TADKFAQWIADAPEKEEIFNIWLGYEAFGSIQKDYTGIFEFLKALPTQTFNRGIGFIT
                     PSEAGKMTPVDTISAPYPLSWSGEEKDLSSWTGNHLQEEALQKLYSVAERVNLCKEKA
                     LKRDWQTIQDTDYLRYMSFKNAWGSTFDSPYDAFINYMNILADFLLRVDAEYPTSIEN
                     EELNALLKTINEQEATISELENTIKTLRSRKTK"
     gene            3624..5153
                     /locus_tag="PK641_03995"
     CDS             3624..5153
                     /locus_tag="PK641_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008672909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HPG55544.1"
                     /translation="MLKKEDLQQLQSKGISESTFYTQLENFEKGFPFADLDSAATIGN
                     GIVTLSENDISKYIKRFDEQKETLSLLKFIPASGAASRMFKDLINFAASYDGSGNLGA
                     YPSIKKCMMNIRKFAFYDKLKSKLRKRDIRIDDLIYNKDYKKIADFILNEYGLDYQHL
                     PKAMLYFHRYPDGPRSAFGEHLVEGSEYAYDINREVNLHFTISPEHRDLFEQKLTTVV
                     LKCEEQFNVKYNIEFSEQKSKTDMVALDQNGELIRNKDGSILFRPGGHGALIENLNDC
                     DADLIFIKNIDNVTVDRLKETTYKYKKALAGYLLFLQEQTFECLEKLDSLSIDSTDLA
                     RIEAFASQQLYIRVNMTYYSQSQREKVAFWQQKLNRPIRVCGMVKNEGEPGGGPYWVR
                     NKQHEKSLQIIEASQIDFTNRFQVESVQKASHFNPVDLVCGVRNYKGNKFNLKNYIDK
                     STGFISNKTQNGIDMKIQELPGLWNGSMANWISIFVEVPIDTFTPVKTLEDLLRDEHQ
                     E"
     gene            5197..>5910
                     /locus_tag="PK641_04000"
     CDS             5197..>5910
                     /locus_tag="PK641_04000"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HPG55545.1"
                     /translation="MNYTKEKQSSTIRFRRFTRKRYAAFTSIHKQVTIGHINVDICLR
                     AQAKAKKLFREQDIRAEFQDEMVENDYSSITPASEELINCLQVCPIVNKHKTEGIGDP
                     RPRSYKAALQLLVCIAALFMFSNIIKASDSIFISSNLSEIEIIAQKSDIYTNKLRPII
                     NLTQNDIKSLPVTSINELLDYIPGIDVRQRGTGGVQADLSLRAGTFDQVLILIDGMDI
                     TDPQTGHHNLDLPIDLTSIE"
BASE COUNT         2083 a          941 c         1129 g         1757 t
ORIGIN      
        1 tgatagattt gaatcaatcg tcaaaacggc tattagagaa attaataagc taatgaacgg
       61 tgaaaattct gagttcatcg aacaaattga tgctcctgat gccttgttgt ggtttatatg
      121 gtcgatacaa cagtatggaa cacatgaatc aaaagaagat atgttccgaa aatatggaga
      181 tatttgcaat gagattatgc agttcatcat tagtcaaaaa catccaaatc tatatttgca
      241 tgataatggt ttattgtatg tagatggtcg tgacactcct atgacatgga tgaatgctac
      301 tgaaaataac aaacctatta cacctagaac aggttatgta gttgaaacaa atgctttatg
      361 gtataatgct ttatgtttta tttctgaata tgctaatcga ctcaaggata ctaaagcaca
      421 aaaagcatac gaatcattgg cagaaaaagt aaaaaagaca tttattcaaa cattctggaa
      481 tggtatctat ttatacgatt ttgtgaatgg cagtgaacat gatttagagg ttcgtccaaa
      541 tatgatattt gcagtatctc taccattttc tcctttagat aaatatcaaa aaaaatctgt
      601 tttagacatt tgtacacgtg agcttctcac aagtaaaggt ttaagaagtt taagccctaa
      661 aagtggttgt tacagaccta attacattgg aggaatgcaa gaacgagacc gcaattatca
      721 caatggacct gtttggccat ggactaccgg tgcatacgca gaagcatatc taatcgtaca
      781 caaacaaagc ggtatatcat ttgttgaacg tatgatgata ggaatggaag cagaaatgaa
      841 gaaattatgc attggtacac tatctgaatt gtacgatggc aatcctccat ataaaggaca
      901 cggtgcaatg tcttttgcac caactgtagc agaaattata cgcaccttaa ccttattaaa
      961 acaatataac gaagcctaaa aacttacgtt aaatggatat attagcaata tgaaagcact
     1021 gatgtttggg tgggagtttc caccgcacat tttaggagga ttgggaaccg caagctatgg
     1081 actaacaaga gggatgtctg tacaagatga cttggaaatt acctttgtaa ttccaaagcc
     1141 atacggagat gaagaccaaa gtttcttaaa actaatagga gcttgcaatg tgcctgttgt
     1201 aagaaaagaa aattcttggg accacgttaa cgaacgcatt ggtagaatca tgcatcctaa
     1261 tgaatatttt tggtatcgtg atcacatata ttacgacttt acacgcattg gaacaaatga
     1321 attaggctgc atagaatttt ccggacgcta tcctgataac ttgattcaag aaatagataa
     1381 ttacgaagca gtcgcaggcg tattagctca tagcttggaa tttgacgtaa tacactcaca
     1441 cgactggctt acatatccgg caggaatttt tgcaaagcaa atatcaggga aaccgcttgt
     1501 aattcacgtg catgcaactg attttgatcg cagtagaggt aacgttaatc ctactgtata
     1561 taatattgaa aaacgtggta tggatattgc tgatcacata atatgtgtaa gcaatttaac
     1621 tagaaacact gtaattgaaa aataccatca acatcctgac aaggtaacaa cagtgcataa
     1681 cgcagtagat cctgttccaa atgtagagca ttttaagaaa gaagaaagaa aagacaaatt
     1741 agttacattc cttggacgta ttaccatgca gaagggaccc gaatattttg ttgaggccgc
     1801 atatagagta ttgcaacgta gcaagaatgt tcgctttgta atggccggaa atggtgatat
     1861 gatgcaagcg atggtagatt tagctgcgtc tcgtggtatt gcagatcgat ttcactttcc
     1921 tggattctta aaaggatcag aggttcatca aatgcttgcc aatagtgatg tttatgtaat
     1981 gccatcagta tcagaacctt ttggtatatc accacttgag gcaatgcagg ttggtactcc
     2041 gtcaattata tctaagcaat caggatgtgc tgaaatatta gcacacgcaa ttaaaacaga
     2101 ttattgggat attgacgcaa tggctgacgc tatttatgct attgttgaat attcttcaat
     2161 atacaaaaca ctaagtgaag agggcataaa agaagttaat tcaatcacat gggacaaagc
     2221 tggattaaaa gttcgcagta tttataatag agttacaggt agataacaaa caaaaccata
     2281 aaatatgaaa aatatttgtt tttattttca ggtacacgta ccttacgcat tacgtcgata
     2341 tcgatttttt gagattggta aagaccacta ttactatgat gattttggaa cagaacaaca
     2401 aattagagaa ttagctgata aatcttattt acccgctaat cggataattg ccaatttgat
     2461 ccgcacatcg ggaggaaaat tctgttgcgg gttttcaatt tccggggcgg ctattgaaca
     2521 attagaacaa tatgcgcccg aggttattga cagttttaaa gaattggcag atacaaattg
     2581 tgttgaattt ctagcagaac cttatgcgca ttcactatct tctgtattta atgataacga
     2641 gtttgaattg caggttaaaa aacattctgc taagataaaa gaactctttg gtaagaaacc
     2701 aacttctttt cgtaattcag agctaattta ttctgatgaa atcggtgaaa aagttgctaa
     2761 actaggatat aacacaatct tgttggaagg tgcaaaacat atattaggat ggaagagtcc
     2821 gaactacgta tatggtcatc cgtatttgcc gaaagtaaaa ttgttgacta gaaacttttc
     2881 tatgagtaac cacatacctt ttgagttttc aaatacttca tgggctgatt atccactaac
     2941 agcagataaa tttgctcagt ggattgcaga tgcccctgag aaagaagaaa ttttcaatat
     3001 atggttggga tatgaagcat tcggttctat acaaaaagat tataccggaa tttttgaatt
     3061 tcttaaagca ttaccgactc aaacgtttaa caggggtatt ggatttatta caccctctga
     3121 agcaggtaaa atgactcctg tagacactat tagcgcacca tatccgttaa gttggtcggg
     3181 cgaagaaaaa gacttaagta gttggaccgg taatcattta caggaagagg ctttacaaaa
     3241 gctatatagc gttgcagaac gcgtcaattt atgcaaggag aaagcgttaa aacgtgattg
     3301 gcagacgata caagatactg attacttaag atatatgagt tttaagaatg cttggggttc
     3361 tacattcgat tcgccttatg atgcgtttat taattatatg aatattttag cagacttctt
     3421 acttagagtt gatgcagagt atcctacatc aatagagaat gaagaattaa atgctctact
     3481 aaaaacaatc aacgaacaag aagcgactat atctgaattg gaaaatacaa tcaagacatt
     3541 gcgtagtcgt aagactaaat aaatattttt aattggagat taagacttaa tgattgatta
     3601 tcacaacatt aatagctaaa aatatgttga aaaaagaaga cttgcagcaa ttacaaagca
     3661 aaggtattag tgagagtaca ttttacacgc agttggaaaa ctttgaaaag ggatttccgt
     3721 ttgctgattt agatagtgct gccactatag gcaatggtat tgttacactt tctgaaaatg
     3781 atatttcaaa atatataaag aggtttgacg agcaaaaaga aacactatct ctgcttaaat
     3841 ttattccggc ttcaggagcc gcatcaagaa tgttcaagga tttaatcaat tttgctgcat
     3901 cttacgatgg atccggcaat ttaggcgcgt atccttcaat taaaaaatgc atgatgaaca
     3961 ttcgaaaatt tgctttctat gacaagctaa aatctaagtt acggaaaagg gatattagga
     4021 tagatgactt gatatacaat aaagattaca agaagattgc agatttcatt ctcaatgaat
     4081 atggtttaga ctatcaacac ctgccaaagg caatgctata cttccatcgt tatcccgatg
     4141 gtccaagaag tgcatttgga gaacacttgg ttgaaggtag cgaatatgct tatgatataa
     4201 atcgtgaggt aaatcttcat tttaccattt cgcctgaaca cagagatttg tttgaacaga
     4261 agctaacaac tgtagtatta aaatgtgaag aacaatttaa tgttaaatac aatattgaat
     4321 tttctgagca aaaatcaaag accgatatgg ttgcattaga tcaaaatggc gaattaatta
     4381 ggaataaaga tggcagtatt ctatttcgcc ccggaggtca tggagcatta attgaaaatc
     4441 taaatgactg tgatgcagac ctaatattta tcaaaaacat tgacaatgtt actgtagatc
     4501 gtttaaaaga aactacatat aagtataaaa aagcattagc aggatatttg ttgtttttac
     4561 aagaacaaac atttgaatgc cttgaaaaat tagattcgct ttctattgat agtacagatt
     4621 tggccagaat tgaagctttt gcatcgcaac aactttatat cagagtaaat atgacttatt
     4681 atagccaaag tcaacgtgaa aaagttgcat tttggcaaca aaaacttaat cgtcctattc
     4741 gtgtttgcgg tatggttaag aatgaaggag aaccgggtgg aggtccatat tgggttcgta
     4801 acaaacaaca cgaaaaaagt ttgcagatta tcgaagcttc acaaatagat ttcacaaaca
     4861 gatttcaagt agaatctgtt caaaaagcat cacactttaa ccctgttgat ttagtttgtg
     4921 gagttcgtaa ctataagggc aacaagttca atcttaagaa ctatatcgat aagtctaccg
     4981 gtttcatttc aaataaaact caaaatggta ttgatatgaa gattcaggaa ttaccgggac
     5041 tttggaatgg atctatggct aattggatta gcatttttgt tgaagttcct attgatacct
     5101 ttacgcctgt aaaaactctt gaagatttat taagagacga gcatcaggag taattcagaa
     5161 aaaaatcata cctttgcgtg gattaatctt aaatatatga actacacaaa agaaaaacaa
     5221 tcaagtacaa ttcgttttcg cagatttacg cgtaaacgct atgctgcttt tacttctatt
     5281 cacaaacaag ttactatcgg acacattaat gttgatattt gtttgagggc acaagcgaag
     5341 gctaaaaaac tttttcgcga gcaagacata agagcagaat ttcaagatga aatggttgaa
     5401 aatgactatt cttcaattac gccggcatcc gaagaactga ttaattgttt acaagtttgt
     5461 cctatagtta ataagcacaa aacagaagga ataggagacc ctagaccacg atcatacaaa
     5521 gccgctttgc aattactagt ttgcatagcg gctttattta tgttttcaaa tatcataaaa
     5581 gcgtcagatt caatttttat tagtagtaat ttgtctgaaa ttgagattat tgcacagaaa
     5641 agcgacatat acacgaacaa acttcggcca ataataaatt tgacacaaaa tgatattaaa
     5701 tcgctacctg ttacatctat aaacgaatta cttgactata tacccggaat tgacgttcga
     5761 caaagaggta caggaggagt tcaagccgac ttatctttga gagccggaac atttgatcaa
     5821 gtattgattt taatagatgg aatggacata accgacccac aaacaggtca tcataattta
     5881 gatcttccaa tagatcttac atctatcgaa
//
LOCUS       DAOPID010000084         5875 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_17856_length_5875_cov_3.729553, whole genome shotgun sequence.
ACCESSION   DAOPID010000084 DAOPID010000000
VERSION     DAOPID010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 5875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5875
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17856_length_5875_cov_3.729553"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PK641_04005"
     CDS             <1..200
                     /locus_tag="PK641_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HPG55546.1"
                     /translation="YGMLVAPGEYEMVLNTDSKSFGGFGLSDESIHHFTMPADGFIDK
                     EWIKLYVPSRSAFVLRRINNK"
     gene            205..912
                     /locus_tag="PK641_04010"
     CDS             205..912
                     /locus_tag="PK641_04010"
                     /inference="COORDINATES: protein motif:HMM:NF003198.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HPG55547.1"
                     /translation="MFSLNKKQLAILIDPDSYGSDKFAHLLSLLSKYTPDYIFVGGSI
                     TSASTEKAIESIKKAVNIPVIIFPGNASQFSPKADALLYLSLISGRNPDYLISQHVNS
                     APYIKRSGISVIPVGYILIESGTMTSVEYISNTKPIPRNKTSIAVATAIAGEFLGQKA
                     IYLEAGSGAQLRVPDKMINSVKQSINIPLIVGGGIRSAEEMKKVFQAGADLVVIGNAL
                     EDNTDLLSELIQTLVLL"
     gene            complement(886..2727)
                     /locus_tag="PK641_04015"
     CDS             complement(886..2727)
                     /locus_tag="PK641_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG55548.1"
                     /translation="MKQIWILLKRFVPPYKINAVGVVSYSMLSTILSLFSFALIVPIL
                     EILFQTREAVYTIQSMDNVQTMQLFDVLKNNLYYYITQFIGVYGPTATLIVLSIALVI
                     MTFLKVITFYASAMSMSKIQSGVLRDLRNTLFSKIVSLPIGFFSEERKGDIMARMSVD
                     VNDVETSIMSSLDMLIKNPLIIIIYLIVMFVISWELSAFVIIMLPISGVLIGQVGKNL
                     KKSSLECAEQGGALNAQVEETLGGLRIVKAFNAESKIEDRYRKLTNKNRESVLKVLRR
                     QQLAHPMSEFLGTTVISIVLCYGGNLILNTSNSPLTAPVFIYYILLFYSIINPIKELS
                     KAAYSVRKGSASLQRIDKILAAKNPLTWPKNSIKLDEFKHQIEYKNVSFSYQTELVLR
                     NVNLTIEKGKTIALVGQSGSGKSTLVDLLPRFWDVTDGEILIDGINIKQMTLHNLRAL
                     MGNVNQEAILFNDSFYNNITFGVENATEEEVIKAAKIANAHDFITATEQGYNTNIGDR
                     GSKLSGGQRQRISIARAILKNPPILILDEATSALDTESEKLVQEALENLMKNRTTIVI
                     AHRLSTIKNADEICVIDNGMITERGTHDELIALNGIYKKLQQDQSLN"
     gene            complement(2764..4110)
                     /gene="purB"
                     /locus_tag="PK641_04020"
     CDS             complement(2764..4110)
                     /gene="purB"
                     /locus_tag="PK641_04020"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HPG55549.1"
                     /translation="MNELTALTAISPIDGRYRGKTNVLAEYFSEYALIRYRVKVEVEY
                     FIALCELPLPQLKSINSNLFKDLRDIYKNFSESDAQAIKDIEKVTNHDVKAVEYFLKE
                     KFDKLGLAEYKEFIHLGLTSQDINNTALPMSVKNCLDDVYYPAIQELIDKLNCLADTW
                     KDIPMLAKTHGQPASPTRLGKEIKVFAIRLEQQLSLLKSVPISAKFGGATGNYNAHLV
                     AYPSYDWKQFGDKFVSEKLGLQRERWTTQISNYDNLAALCDGLKRINTIIIDLDRDFW
                     TYISMEYFKQTIKAGEVGSSAMPHKVNPIDFENSEGNLGLSTAILEHLAHKLPVSRLQ
                     RDLTDSTVIRNMGVPFAHTLISIQSTMKGLGKLILNEPALERDLEHCWNVVAEAIQTI
                     LRREGYPKPYETLKELTRTNSTVTEESIHAFIETLNVSESVKAELRQISPFNYTGM"
     gene            complement(4114..5475)
                     /locus_tag="PK641_04025"
     CDS             complement(4114..5475)
                     /locus_tag="PK641_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HPG55550.1"
                     /translation="METSVPAWTLIPFGMMLMCIAIAPLFLEKSWEKNLNKLIVSLVL
                     GLPTAAYMIYIGLGEKVAHQLMFDYVPFIILLCSLFAVTGGIHLSGDIAAKPKINTLF
                     LGIGYVLASIMGTTGAAMLLIRPIIATNAQRKHKTHTILFFIAAVANCGGLLTPLGDP
                     PLFLLYLRGAEFTWFLNLLPEWAFVGALLLGIYFLVDTYYYKKENLADIKKDNTQKSP
                     IRISGNVNFLYLVGIVLSVAFINPGTIPGMADHDGYTANLVMKYLREIVLVLIILGSI
                     FTTKKDIRIANNYSWSPIIEVAALFLGIFITMTPALLFLQEHAASLGLDKPWQFYYAT
                     GALSSFLDNAPTALAFHSVASGLPESIAAANANIVDGLLNFNGNDVSFVARVPETLLK
                     AISIGAVFFGAMTYIGNGPNFMVKAIAEENKISMPSFFGYMIKFSLIILLPIYIITQL
                     IFF"
     gene            complement(5501..>5875)
                     /locus_tag="PK641_04030"
     CDS             complement(5501..>5875)
                     /locus_tag="PK641_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HPG55551.1"
                     /translation="INGFRPSKLVKLDILLNGEPVDALSSLTHADNAVTIGRRMCEKL
                     RELIPRQQFEIAIQAAIGAKIIARETIKPVRKDVTAKCYGGDISRKRKLLEKQKKGKK
                     RMKQIGSVEVPQKAFMEVLKLD"
BASE COUNT         1891 a         1069 c          968 g         1947 t
ORIGIN      
        1 attatggtat gttagttgct ccaggtgaat atgagatggt acttaatact gattctaaat
       61 catttggtgg attcggttta agcgacgaat ctatacatca ctttacaatg cctgctgatg
      121 gctttataga taaagaatgg attaagctat acgtgccatc acgttctgct ttcgttctac
      181 gtcgtattaa taacaaatag gatagtgttt tcactaaata aaaaacaatt agcaatactt
      241 atcgatcccg attcttatgg gagcgataag tttgcgcatt tgttatctct cttgtctaaa
      301 tacactcccg attacatttt tgttggtggc agcattacat ccgcatctac agaaaaggcc
      361 atagaatcaa taaaaaaagc agtaaatata cctgtaataa tatttcccgg taatgcttct
      421 cagtttagtc ctaaagcaga tgcattattg tatttatccc ttatttcagg tcgaaatccc
      481 gattacttaa tatctcaaca tgtaaattca gctccatata ttaagcgttc cggaataagt
      541 gttataccgg tcggatatat attgattgag agtggcacta tgacaagtgt tgagtacatc
      601 agcaatacta aaccaattcc tcgtaataag actagtattg cagtagcaac agctatagcc
      661 ggcgaatttt taggtcaaaa agctatttat ttagaagcgg gtagtggtgc tcaattaaga
      721 gtaccagata aaatgataaa ctctgtaaag cagtcaataa acattccatt aattgttggt
      781 ggaggaataa ggtctgcaga agaaatgaag aaagtttttc aagcaggagc agatttagtc
      841 gtaattggta atgctctcga agataatacc gatttactat ccgaattaat tcaaactttg
      901 gtcttgttgt aacttcttat agatgccatt aagagcaata agctcgtcgt gtgtacctct
      961 ttcggtaatc ataccattat caataacgca tatctcatca gcattcttta tagttgataa
     1021 gcgatgtgca attacaattg ttgtacgatt cttcatcagg ttctctaaag cctcttgtac
     1081 aagtttttcc gattcagtat ctaaggccga tgtggcttcg tctaaaatta aaataggagg
     1141 gttttttaaa atagctcttg ctatactaat gcgttgacgt tgtccgccgg ataatttact
     1201 tcctctatcc ccaatattag tattatatcc ttgttctgtg gcagtaataa aatcatgtgc
     1261 atttgctatt ttggcagctt taattacctc ttcttcggta gcattttcaa cgccaaaggt
     1321 aatgttattg taaaacgaat cattaaataa tatggcttcc tgatttacat tacccataag
     1381 tgcacgtaaa ttgtgtagag tcatttgttt gatattaatg ccatcaatca aaatctctcc
     1441 atccgttaca tcccaaaaac gtggtaataa atcaaccaaa gtagatttgc ccgaacctga
     1501 ttgacctact aaggctatgg ttttaccttt ttcaatagta agattaacat tgcgaagtac
     1561 caattccgtt tggtatgaaa aactgacatt cttatattct atttgatgtt taaattcgtc
     1621 aagtttaatc gaatttttag gccatgttaa tgggtttttt gcagctaata ttttatcaat
     1681 acgttgcagt gatgctgaac ctttacgaac gctatatgca gctttagata gttctttaat
     1741 agggttaatt atactataaa acaacaagat ataatatatg aatacgggag ctgttaatgg
     1801 tgagttactc gtatttaaaa tcaagttacc tccataacat agaacaatag atattacagt
     1861 cgttcccaaa aattcgctca ttggatgagc caactgttgg cgacgtaaaa cttttaatac
     1921 agattctcta ttcttgttag ttagttttct gtatctgtct tctattttcg attctgcatt
     1981 aaatgccttt acaatacgta aaccacctaa tgtttcttca acttgtgcat ttaaggctcc
     2041 tccttgttct gcacattcca aagaactttt ctttaggttt ttaccaactt gcccaatcaa
     2101 aacaccacta ataggcagca ttataattac aaaagcagat aattcccaac ttattacaaa
     2161 catcacaatc aaataaataa tgataataag cgggtttttt attaacatgt caagcgaact
     2221 catgattgac gtttcaacat cgttcacatc gacgctcata cgtgccatga tatcaccctt
     2281 acgctcttcg ctaaaaaatc cgattggtaa acttacgatc ttactaaata aggtgttacg
     2341 taggtctcgc aaaactcctg attgtatttt tgacattgac atggcacttg cataaaaagt
     2401 aataactttt aagaatgtca tgattacaag agctattgat aatacaatca gtgttgctgt
     2461 cggaccataa actccaataa actgagttat gtaatagtat agattattct ttaaaacatc
     2521 aaacaattgc attgtctgca cattgtccat actttgaatt gtatatacag cctcacgagt
     2581 ctgaaaaaga atttccagaa taggaacgat tagtgcaaaa gaaaacaagc taagtatagt
     2641 tgacaacata ctatacgaca caacacctac cgcattaatt ttgtaaggcg gcacaaagcg
     2701 ttttagtaat atccaaattt gtttcacttt cttgtgttgt tagtgcttgc tgtaaatact
     2761 taattacatt ccggtgtagt tgaacggact tatttgtctc aattcagctt ttacactttc
     2821 agatacattt aatgtttcaa taaaggcatg tattgattct tcagtaactg tactattggt
     2881 acgagttaat tctttcagtg tttcgtaggg ttttggatat ccttctctac gtaaaattgt
     2941 ttgaatagcc tctgcaacaa cattccagca atgttccaaa tcacgttcta aagcaggttc
     3001 attcaaaatt aatttaccta aacctttcat tgtgctttgt atcgaaatca atgtgtgagc
     3061 aaaaggtact cccatgtttc taataacagt agagtcagtt aagtcgcgtt gtaaacgtga
     3121 tacaggtaat ttgtgagcca aatgctccaa aattgcggtt gataagccta agttaccttc
     3181 cgaattttca aaatcaatag gatttacttt gtgtggcatt gcagatgaac caacttcacc
     3241 ggcttttatt gtctgcttaa agtattccat tgagatatat gtccagaaat cgcgatctaa
     3301 atcaatgata atagtgttga ttcgttttaa accatcgcaa agagcagcta aattatcata
     3361 gttagatatt tgagtcgtcc atctttctct ttgtaatcct agtttttcac ttacaaactt
     3421 atccccaaat tgtttccaat cgtagcttgg atatgcaaca agatgtgcat tgtaatttcc
     3481 ggttgcgccg ccaaattttg cagatatagg aactgattta agcaaactta actgttgttc
     3541 aagtctaatt gcgaacacct taatctcctt acccaaacgg gtaggagatg caggttgtcc
     3601 gtgtgtcttt gccaacatgg gaatatcttt ccatgtgtca gctaagcaat ttaatttgtc
     3661 aattaattct tgaatggcag gataatatac atcatccaaa caattcttta cagacatagg
     3721 caaggcagtg ttatttatgt cttgcgatgt caatcccaaa tggatgaatt ctttgtattc
     3781 ggccaaacca agtttgtcaa atttttcttt taaaaaatat tctaccgctt taacatcgtg
     3841 attagtaacc ttttcaatgt cttttattgc ttgtgcatca gactcagaaa agttcttgta
     3901 aatatctctc aaatccttaa atagattact atttattgat ttaagttgtg gtaatggtaa
     3961 ttcgcataga gcgataaaat attccacttc tacctttaca cgatagcgga ttaaggcata
     4021 ttcagaaaaa tattccgcta atacatttgt ctttccacga taacgtccgt caatgggcga
     4081 tatggctgtt aaagcagtta attcgttcat tatttaaaag aaaatcaatt gagttataat
     4141 ataaatcggt aataaaatta tcaaagagaa ttttatcatg tatccaaaga aactaggcat
     4201 tgaaattttg ttctcttcag cgatagcttt aaccataaag tttggtccgt taccaatata
     4261 ggtcatagca ccaaaaaata cagcaccaat agaaatggct tttagcaagg tttcaggaac
     4321 ccgcgcaaca aatgatacat catttccgtt aaaattcaac aaaccatcaa caatgtttgc
     4381 attagctgct gctatagatt caggtaaacc gctagctaca ctatgaaatg caagtgctgt
     4441 aggagcgtta tcaaggaaag agcttaaagc acctgttgca taataaaatt gccaaggttt
     4501 atccaaacct aaacttgcag catgttcttg taagaacaat aatgcaggag tcatagttat
     4561 aaaaatacct aagaataatg cagctacttc aattatagga gaccatgaat aattgtttgc
     4621 gatgcgaata tcttttttag ttgtaaatat agatcctaat atgataagaa ctaaaacaat
     4681 ttcgcgtaaa tatttcatta caagattagc tgtatatccg tcatgatcag ccattcccgg
     4741 aattgtaccc ggattgataa atgctacgga taatacaatt cctaccaaat ataagaaatt
     4801 tacatttccg cttatacgaa taggtgactt ttgggtattg tcttttttga tgtcagcaag
     4861 attttctttc ttataatagt aagtatcaac taagaagtaa attcctaata ataaagctcc
     4921 tacaaaagcc cattccggta ataaatttaa aaaccatgta aattcagcac cacgtaaata
     4981 aagtaagaat agtggaggat cgccgagtgg agttaataaa ccaccacagt tagcaaccgc
     5041 tgcaataaag aacaaaatag tatgtgtttt gtgtttacgt tgtgcatttg ttgcaataat
     5101 aggtctgatt aaaagcattg cagcacctgt tgttcccata attgatgcca aaacgtaacc
     5161 aatacccaaa aacagagtat taattttagg ttttgcagcg atatcaccgc ttaaatgaat
     5221 acctcctgtt acagcaaaca gagagcaaag taaaatgata aaaggcacat aatcaaacat
     5281 taattgatgt gcaacttttt caccaaggcc tatgtatatc atataagccg cagtaggtaa
     5341 acctaaaaca agtgatacaa tcaacttgtt aagatttttc tcccaactct tttctaagaa
     5401 gagtggtgcg atcgcaatac acattaacat cattccaaag ggaatgagcg tccatgcggg
     5461 aaccgatgtc tccataattt tctaagttaa tatttaatat ttaatccaat ttaagtactt
     5521 ccataaaggc tttttgaggc acttcaacag aaccaatctg tttcatacgt ttcttgcctt
     5581 ttttctgttt ctcaagcaac ttgcgtttac gcgagatatc acctccgtaa catttggcag
     5641 ttacatcttt tcgtacaggt tttattgtct ctcgtgctat aattttagct ccgatagctg
     5701 cttgtatagc aatttcaaat tgttgacgag gaatcaattc ccttaacttt tcacacattc
     5761 ttcgtcctat tgtaacagca ttatcagcat gggttaaaga agataaagca tctacaggtt
     5821 ctccattaag taagatatcc aattttacta attttgaagg acgaaatccg tttat
//
LOCUS       DAOPID010000085         5768 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_18316_length_5768_cov_4.572554, whole genome shotgun sequence.
ACCESSION   DAOPID010000085 DAOPID010000000
VERSION     DAOPID010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5768
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18316_length_5768_cov_4.572554"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            297..467
                     /locus_tag="PK641_04035"
     CDS             297..467
                     /locus_tag="PK641_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55552.1"
                     /translation="MKRSHRISFMLNDKEVDALERYIKKYKIKCKSKFVREALMITVI
                     RKLEEDSPTLFD"
     gene            470..904
                     /locus_tag="PK641_04040"
     CDS             470..904
                     /locus_tag="PK641_04040"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HPG55553.1"
                     /translation="MYDDNYFMKLALIEAKTAFEAGEVPIGAVVVQQNRVIGRGHNLT
                     ETLEDATAHAEMQALTAASGTLGAKYLIDCTIYVTVEPCVMCAGAMSWTKISRLVYGA
                     SEPKFGYTQKAPTALHPKTEVLAGLMADECSELMKRFFKDKR"
     gene            906..2195
                     /gene="tyrS"
                     /locus_tag="PK641_04045"
     CDS             906..2195
                     /gene="tyrS"
                     /locus_tag="PK641_04045"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPG55554.1"
                     /translation="MNFVEEIKWRGMLHSIMPGTEEQLQKEMTTAYLGIDPTADSLHI
                     GHLCGVMMLRHFQHCGHKPLALIGGATGMIGDPSGKSAERNLLDEATLRRNEEAIKKQ
                     LSHFLDFTGEAPNKAEMVNNYDWMKGFSFLDFAREIGKHITVNYMMAKESVQKRLNGE
                     ASDGLSFTEFTYQLLQGYDFMHLYETKNCKLQLGGSDQWGNITTGTELIRRKLGGSAF
                     ALTCPLITKADGGKFGKTESGNVWLDRRYTSPYKFYQFWLNVSDDDAEKYIKIFTSLT
                     REEIESLVEEHKTAPHLRILQKRLAQEVTIMVHSKGDYDMAVEASSILFGGSTSETLR
                     RLDEDTLLSIFDGVPRFDITLSENLGLKFVDILTEKASVFESKGEMRKLVQNGGFFIN
                     KEKVANADIVFDETNLLNGKYILLQKGKKNYFLLTAK"
     gene            complement(2435..2508)
                     /locus_tag="PK641_04050"
     tRNA            complement(2435..2508)
                     /locus_tag="PK641_04050"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2472..2474),aa:Ile,seq:cat)
     gene            2583..3176
                     /locus_tag="PK641_04055"
     CDS             2583..3176
                     /locus_tag="PK641_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPG55555.1"
                     /translation="MIKNIVFDMGGVLIDLDMQASVKSFKNLGFENVEEYISPYTQSG
                     FMLDFERGLMSNADFCNEVRKLSGKTVSDKQIEDAWFQFLLDIPAEKLKLIKNLREKY
                     MIYLLSNTNWIHFPVMLERYNLDKYFDKFYLSCEVHYTKPDPRIFEYMINDAGIKAEE
                     TLFIDDGPANIKTAESLGFQTHLAKANESLEFLNAII"
     gene            3276..4217
                     /gene="ribF"
                     /locus_tag="PK641_04060"
     CDS             3276..4217
                     /gene="ribF"
                     /locus_tag="PK641_04060"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HPG55556.1"
                     /translation="MKIIDNIANFDCSASAVTIGFFDGVHRGHVALLHKLTKEAHKRS
                     LASVVVTFAIHPREGLHANYVPKLLTTHAERMQLLEKQGVDICVLLKIDHKMFEMSSG
                     SFMHHVLFEKLNTKYLLVGHDHHFGCDRENGYVHYKECGNKYGIEVEQANPFPGNGLN
                     ISSSLIRKALEEGNINQASEWLGYRFYIEGTIVQGHKIGRSIGFPTANIAINDVRKLI
                     PHEGVYAVDVELEGEKEILHGMLNIGIRPTFVTVTVAKSIEVNIFDFNQDIYHKKIKV
                     FFLDYLRPEIKFDSPEKLTAQLNDDREAVLKWFEQKL"
     gene            complement(4277..>5768)
                     /locus_tag="PK641_04065"
     CDS             complement(4277..>5768)
                     /locus_tag="PK641_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPG55557.1"
                     /translation="DWCGVNALNTIRKEGWRSVMINYNPETVSTDYDSCDRLYFDELS
                     FERVLDILDLENPHGVIVSTGGQIPNNLAIRLDCMNVPILGTSAKNIDNAEDRHKFSA
                     MLDRIGVDQPRWKELVSLDDINKFVNEVGFPILVRPSYVLSGAAMNVCSNESELIEFL
                     QLAANVSKKHPVVVSSFIERAKEIEMDAVADHGEIITYAISEHVEFAGVHSGDATIQF
                     PPQKLYLETMRRIKRISKQIAKELDISGPFNIQFLARNNEIKVIECNLRASRSFPFVS
                     KVLKINFIELATKIMLGIPVEKPNKNAFNLDYVGIKASQFSFSRLQKADPVLGVDMAS
                     TGEVGCLGDDTNEAILKSMLSVGYKIPEKNIMLSTGSAKQKVDMLDAARLLQEKSYHL
                     YATGGTQKFLQDNGIKSERIYWPNETDKQPQALDLIREKKIDLVVNIPKNLSADELDN
                     GYKIRRAAIDFNIPLLTNPRLASAFINAFCNININNIEIKSWSEYK"
BASE COUNT         1818 a          903 c         1164 g         1883 t
ORIGIN      
        1 tgtaaataat ttacagcagc gttttctccg tccttgccga gtttattatg ttcagccatg
       61 atgtaaataa gaattaagta gttaaaaacg ccccaaaatt acatataaat ttctatttaa
      121 acagtactct cgcttggaaa tgcttactga tgagagtttt gttaatcaat tgatatgcaa
      181 atattaatcg atttgaattt ctgtaatcgc aaaaaactat gtacttttgt agttcaaatt
      241 aagtgttcgt gtctataatg cagtccaata aaaataaaat acaaaagatt aggaagatga
      301 aacgcagtca tcgcataagt tttatgctga atgataaaga ggtggatgca ttagaaaggt
      361 atatcaaaaa gtacaaaata aaatgtaaat cgaagtttgt gcgcgaagct ttgatgataa
      421 ctgtaataag aaaattagaa gaagattctc caacactatt cgattgatga tgtacgacga
      481 taattatttc atgaaattag ctttgattga ggctaagacc gcttttgaag ccggagaagt
      541 tcctattgga gctgttgttg tgcagcagaa tagagtgata ggcagagggc ataatttaac
      601 ggagaccttg gaggatgcaa cagctcatgc agaaatgcag gccttaacag ctgcatcagg
      661 aacacttggt gctaaatatc tgattgattg tacaatatat gtcacggttg agccttgtgt
      721 gatgtgtgcg ggtgctatgt cgtggacaaa aatttcgaga ttggtatatg gagcatccga
      781 acccaagttc ggttatacac agaaagcccc tacagcccta catcctaaaa cagaagtttt
      841 ggccgggctt atggctgatg aatgttctga attaatgaaa cgttttttta aagataaaag
      901 ataagatgaa ttttgtagaa gaaattaaat ggagaggtat gttgcatagc atcatgccgg
      961 gtactgaaga acaattacaa aaagaaatga caactgcata tttggggata gacccaactg
     1021 cggattcatt acatataggc catttatgtg gtgtgatgat gttgcgccac tttcagcatt
     1081 gcggacataa accattggct ttgattggcg gagcaacggg aatgataggc gatccatccg
     1141 gcaaaagtgc tgaacgtaac ttgcttgatg aggcaacact tcgtcgtaat gaagaagcta
     1201 taaaaaagca attatctcat tttcttgatt ttaccggaga agctccaaat aaggctgaaa
     1261 tggtaaataa ttatgattgg atgaagggtt tttctttcct tgattttgct cgtgaaatag
     1321 gaaagcacat cacggttaat tatatgatgg caaaggaatc tgtacaaaag cgtcttaatg
     1381 gtgaagcaag tgatggtttg tcatttacgg aatttacata tcagttatta caaggatacg
     1441 attttatgca tttgtacgaa accaagaatt gtaaattaca acttggaggt tcggatcaat
     1501 ggggcaacat tactaccggt acggaactta ttcgtagaaa attgggaggt agtgcatttg
     1561 ctttaacatg tccgcttata actaaggctg atggcggtaa gtttggtaaa acagaaagtg
     1621 gtaatgtgtg gttggacaga cgttatacat ctccatataa gttttatcag ttttggttaa
     1681 atgttagtga tgatgatgcg gaaaaatata ttaagatatt cacatcattg acacgtgagg
     1741 agatagaatc gttagttgaa gaacataaga cagctccgca tttgcgcatc ttgcaaaaac
     1801 gtttagctca agaagtaact atcatggttc attcaaaagg agattatgat atggctgttg
     1861 aggcatcttc aatattgttt ggaggaagta cttctgaaac tttacgtcgt ttggatgaag
     1921 atactttatt atctattttt gacggagttc cgcgttttga tattacttta tctgaaaact
     1981 taggacttaa atttgttgat atcttgactg aaaaagcatc agttttcgaa tctaaaggtg
     2041 agatgcgtaa gttggttcag aatggtggtt tctttatcaa taaagagaag gtagcaaatg
     2101 ctgatatagt atttgatgag acgaatttgc ttaatggtaa atatattcta ttacagaaag
     2161 gtaaaaagaa ttatttttta ctgacagcaa aataaaagat aagattttgc gcaattttta
     2221 ggaatttgta ttatctttgc cgcgcaaatg cgataaccgc aatagtaatt gtctcatggt
     2281 gtaatggtag cactgcagtt tttggttctg cctgtcgaag ttcgaatctt cgtgagacaa
     2341 caaacctgat cattttatgg tcaggttttt ttgtggaaaa atttgatttt gtgaaataaa
     2401 aaaagcggct gatgtaaatc aaccgctttg caggtgggcc cacttggact tgaaccaagg
     2461 acctgcgggt tatgagtccg atgctctaac caactgagct ataggccctg acttgagata
     2521 tttttacgta tctttgcaaa tcggaggcaa aggtaatttt tttttattaa gttacaaaga
     2581 aaatgataaa aaatattgtg tttgacatgg gtggtgtgct gattgatttg gatatgcagg
     2641 caagcgtaaa atcgtttaag aatttaggtt ttgagaatgt tgaagagtat attagtccat
     2701 acactcagtc tggttttatg ttggattttg agagagggtt gatgtctaat gcggatttct
     2761 gtaatgaggt aagaaaacta tccggaaaga ctgtttctga caaacaaata gaagacgctt
     2821 ggtttcagtt tttattggat attcctgccg agaagctgaa acttataaag aatttgcgcg
     2881 agaagtatat gatatacctt cttagcaata caaattggat acactttcca gttatgttag
     2941 aacgttataa tcttgataaa tattttgata agttttattt gtcttgtgaa gtgcattata
     3001 caaaaccgga tcctcggata tttgagtata tgataaacga tgccggaata aaagcagaag
     3061 agaccttgtt tattgatgac ggacctgcaa atattaagac ggcagaatct ctcggatttc
     3121 aaactcattt ggctaaggca aatgagagtc tggagttttt gaacgccata atttaaggct
     3181 tgtgttaaat tatagctagc agatatttag atacatatta ttttttttgc ttaaaagttg
     3241 tttttcgcta gtcttacact atctttgcct ccattatgaa aataattgat aacatagcta
     3301 attttgattg tagcgcatca gccgttacta tcggtttttt tgatggtgta cacagaggcc
     3361 atgtggcact attgcacaaa ttaaccaaag aagcacacaa acgttcactg gcttcggttg
     3421 tagtaacatt tgctatccat ccgcgcgagg gtttgcatgc caattacgtt cctaaattgt
     3481 taactacaca tgcagaacgt atgcaattac ttgaaaaaca aggtgttgat atctgtgttt
     3541 tgttgaagat tgatcataaa atgtttgaga tgtcgtctgg ttcatttatg catcacgtgt
     3601 tatttgaaaa acttaatacc aagtatttac ttgtaggtca tgatcatcat tttggttgcg
     3661 atcgtgaaaa tggctatgtt cattacaaag aatgtggaaa taaatatggt atagaagtag
     3721 aacaagcaaa tccattcccc ggaaatggat taaatataag ttcttcgtta attcgtaaag
     3781 cccttgaaga aggaaatata aatcaagcat cagaatggct gggatatcga ttttatattg
     3841 aaggcactat tgtacaaggt cataagatag gtcgttcaat aggattccca acagcaaata
     3901 ttgctattaa tgatgtgcgc aaattaatac ctcatgaagg agtttatgca gtagatgtgg
     3961 aattggaagg agaaaaagaa atattgcacg gcatgcttaa tattggaatt agacctacct
     4021 ttgttacagt tacagtagct aagagcattg aagtgaatat ttttgatttt aaccaagata
     4081 tatatcataa aaaaataaaa gtattttttc ttgattattt gcgtccagag attaagttcg
     4141 attcgcctga aaaattgaca gcacaactta atgatgatag agaagctgtt ttgaagtggt
     4201 ttgaacaaaa actataatat attttgtaat tatattataa gccccgaaag tttgattaaa
     4261 gctttcgggg cttgttttat ttatactcag accaactctt tatttcaata ttattgatgt
     4321 tgatattgca aaatgcgttt ataaatgcag atgcaagacg tggatttgtg agtagaggaa
     4381 tattaaagtc tattgcagct cgccggattt tgtatccgtt gtcgagttcg tctgcactta
     4441 gattctttgg aatattcaca accaaatcaa tcttcttttc gcgtattaag tcaagcgctt
     4501 gaggctgctt atcagtttcg ttaggccaat aaatacgttc agacttaatt ccattatctt
     4561 gtaagaactt ctgagtccct cctgtggcgt ataaatgata tgatttttct tgcaaaaggc
     4621 gtgcagcatc taacatatct actttttgtt ttgcacttcc tgttgatagc ataatattct
     4681 tctcaggaat tttgtatcct actgacagca ttgattttaa aatagcttca ttagtgtcat
     4741 ctcctaggca tccgacttcg cctgtagatg ccatatcaac tcctaatacc gggtctgcct
     4801 tttgcaatct ggaaaacgaa aattgtgagg cctttattcc tacatagtct aggttaaatg
     4861 cgtttttatt tggcttttct acagggatac caagcattat ttttgtagca agttcaatga
     4921 aatttatttt cagaacttta gatacaaaag ggaatgatcg tgatgcgcgt agattacatt
     4981 caataacttt tatctcattg ttgcgagcca aaaattgtat gttgaaagga cccgaaatgt
     5041 ctagctcttt tgcaatttgt tttgatattc tctttatacg acgcatcgtt tcaagataca
     5101 atttttgtgg ggggaattgt atagtcgcgt caccggaatg aacacctgca aactctacgt
     5161 gttctgaaat agcgtatgtg ataatttcac catggtcggc aactgcatcc atctctattt
     5221 cctttgctcg ttcaataaaa gaacttacta ctacaggatg tttttttgaa acatttgccg
     5281 ctaattgtaa gaactcaatc aattcgcttt cgtttgaaca tacattcatg gcagctcctg
     5341 atagcacata gctgggacga actaaaatag gaaaacctac ttcattaaca aacttattaa
     5401 tatcatccaa acttactaat tctttccacc ttggctgatc aacgccaatt ctatcgagca
     5461 tcgcagagaa tttgtgtcta tcttctgcat tgtcaatgtt ttttgcagat gtacctagta
     5521 ttggtacatt catacagtct aatctaatcg ctagattatt aggaatctgg ccacctgttg
     5581 aaacaataac accatgagga ttttccaaat ccaagatatc taaaactctt tcaaaggata
     5641 attcgtcaaa atataatcta tcgcagctgt cgtaatcggt cgaaacggtt tcaggattat
     5701 aattaatcat aaccgaacgc caaccttctt ttcttattgt atttagagca tttacgccgc
     5761 accagtca
//
LOCUS       DAOPID010000086         5699 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_18623_length_5699_cov_3.234940, whole genome shotgun sequence.
ACCESSION   DAOPID010000086 DAOPID010000000
VERSION     DAOPID010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18623_length_5699_cov_3.234940"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="PK641_04070"
     CDS             complement(<1..805)
                     /locus_tag="PK641_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HPG55558.1"
                     /translation="MTHMYEASELILNQDGSVFHLHLKPEQLSDKVILVGDRARTDVI
                     ASYFDSIECEVQNREFRTITGMYRNKRISVLSTGIGCGNLDIVMNELDALVNIDLEKR
                     VNKDVFKKLTLVRVGTSGGLQANVPEGTFVASKRSIGFDGVINFYADSTKVCDMQFEK
                     AFVDYTNFSKRHAAPYCVKNSENLLTRIASTDMVLGNTIASSGFYGPQGRILRLNLDD
                     ANLNKKIMQFEFNGEKITNYEMEGSVLAGMSALMGHEALTVCLIIANRYG"
     gene            1253..2122
                     /gene="purU"
                     /locus_tag="PK641_04075"
     CDS             1253..2122
                     /gene="purU"
                     /locus_tag="PK641_04075"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HPG55559.1"
                     /translation="MENTNKDTAVLLLHCPDRQGILASVTDFINVNKGNIIYLDQYVD
                     TIQKAFFMRVEWELESFLIPKEKIKEYFETLMAKKYSMDFQLYLHAQKPRMAIFVSKQ
                     SHCLYDLLAHYEAGDWNVEIPLIISNHPDMEHVAKKFNIPYYCFPITKETKEEQEAKE
                     LALLKENNISFLVLARYMQVISPNFIRAYPEKIINIHHSFLPAFVGARPYHAAYERGV
                     KIIGATSHYITEELDAGPIIKQDVTGISHKDTVENLIRKGQDLEKIVLSRAVEKHIEH
                     KILVYKNKTIVFG"
     gene            2127..2717
                     /gene="hisH"
                     /locus_tag="PK641_04080"
     CDS             2127..2717
                     /gene="hisH"
                     /locus_tag="PK641_04080"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005788823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HPG55560.1"
                     /translation="MNIAIVKYNAGNIYSVQHALRRLGIEPVVTDDIQILRSADKVIF
                     PGVGEASTTMSYLQNKGLDKLICSLTQPVLGICLGMQLMCAYSEEGGKTESLGIFDAP
                     VKLFIPQNREKIPHMGWNQIYNLKSPLFAGTKENDYVYFVHSYYVPVCKDTIASCDYI
                     EAFSAALHKNNFYATQFHPEKSGSIGSKILEHFLAL"
     gene            2714..3457
                     /gene="hisA"
                     /locus_tag="PK641_04085"
     CDS             2714..3457
                     /gene="hisA"
                     /locus_tag="PK641_04085"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HPG55561.1"
                     /translation="MIQLIPAIDIIEGKCVRLSQGKYDQKKVYNENALEVAKEFEAHG
                     ITRLHLVDLDGAKAKHIVNYKVLENIASNTNLCIDFGGGLKTDEDLKIAFECGASMVT
                     GGSIAIKDAATFSSWINIYGKDKIILGADVKGETIALNGWTEESDCQLMPFLDSYIQK
                     GIDKVICTDIAKDGMLEGPSIDLYKKILSKHKNLYLIASGGVSSMEDIAALESAGLPA
                     VIFGKAIYEGRISLKQLESSIIQKHIIIC"
     gene            3451..4203
                     /gene="hisF"
                     /locus_tag="PK641_04090"
     CDS             3451..4203
                     /gene="hisF"
                     /locus_tag="PK641_04090"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HPG55562.1"
                     /translation="MLAKRIIPCLDVKDGKTVKGINFVNFRDAGDPVELGAKYSEEGA
                     DELVFLDITASHEGRKTFVDMVKRVAANISIPFTVGGGINELSDVERLLNAGADKVSV
                     NSAAIRNPQLINDIARNFGSQVCVAAIDAKYDDGVWTCYLNGGRIPTERSLFEWAKEA
                     QDRGAGEILFTSMSHDGVKTGYPNDALATLADTLTIPVIASGGAGTKEHFKDAFLIGK
                     ADAALAASVFHFGEIKIPELKEYLRNNNIPVR"
     gene            4200..4826
                     /gene="hisIE"
                     /locus_tag="PK641_04095"
     CDS             4200..4826
                     /gene="hisIE"
                     /locus_tag="PK641_04095"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HPG55563.1"
                     /translation="MKLDFAKQGGLIPAIIQDNVTNKVLMLGYMNEESLAKTEEIGKV
                     TFFSRSKQRLWTKGEQSGNFLNVVSIKEDCDSDTLLIKVNPEGPVCHTGSDTCFDEVN
                     QMDDLMFLKHLQDYIDKRYKEMPEGSYTTSLFKDGVNRMAQKVGEEAIETVIEATNGN
                     NNRLIYEASDLIYHLMVLLTSKGLRIEDLAIDLKKRHKKSSNEYPHQV"
     gene            4804..5475
                     /locus_tag="PK641_04100"
     CDS             4804..5475
                     /locus_tag="PK641_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPG55564.1"
                     /translation="MNTLIKYNNVDIYQKEQLVLKNVNLEIGTGEFYYLIGKVGSGKS
                     SLLKTMYAELPIENGEANIFEYNLIDIRNKDIPHLRRQIGIIFQDFQLLNDRTVKENL
                     LFVLRATDWKDKDDMDKRIEEVLSEVGMSTKGYKMPHELSGGEQQRIVIARALLNKPK
                     LILADEPTGNLDPQAGKDLVELLQKICHSDSGTTIIMATHNLGWLDSYPGKVLSIEKD
                     TLTES"
BASE COUNT         2038 a         1009 c         1045 g         1607 t
ORIGIN      
        1 tgccgtatct attagcaata atcaagcaaa cagttagtgc ctcatgcccc attagtgctg
       61 acatgccggc aagtacagag ccttccatct cataattagt aatcttttcg ccattgaatt
      121 caaattgcat aattttctta ttcaaatttg catcatccaa attcaaacgc aatatcctac
      181 cctgaggtcc gtaaaaaccg ctcgaagcta tggtattacc caataccata tcggttgatg
      241 caatacgagt aagtaagttc tccgagtttt taacgcaata aggagcagca tgacgtttcg
      301 aaaaattagt ataatcaaca aaagcctttt caaactgcat atcgcaaacc ttggtactat
      361 ctgcgtaaaa attaatcaca ccgtcaaaac ctattgaacg tttggatgca acaaaagtac
      421 cttccggcac attagcttgc aatcctccgg acgttcctac cctgactaaa gtcaatttct
      481 taaacacatc cttattaaca cgtttctcca aatcaatatt tacaagagca tcaagttcat
      541 tcatcacaat atccaaatta ccacaaccaa ttcctgtaga taatacgcta atgcgcttat
      601 tgcgatacat tccggtaata gttctaaact cacgattttg cacctcgcac tcaatgctgt
      661 caaaatatga agctataaca tcagttctag ccctatcccc aaccaatatc accttatcag
      721 ataattgctc aggtttcaga tgcaagtgaa acacactccc atcctgattt aaaatcaatt
      781 cagatgcttc gtacatatgg gttatgggtt atgggttatt tattagttta caaagtacta
      841 taaaaaacac gagataaaaa aattcaatta taaagaaaat ccaataaaac agatgattcg
      901 tacatatgtg gaatcgtgta atgtgtaatg tgtataatcc aatgttgaaa tgattaataa
      961 tgcgtcgtag gggcatgcca tggcatgccc ttcatttgat gatgcgatac cgtttaataa
     1021 atgcgtcgta ggggcgaatt gcattcgccc ttcatatgaa atacgcaacg atttatcgaa
     1081 caacgtgtat ataattatac atttcaacat tgaattataa atattacatg tcgaaacatt
     1141 tgtgtatgtc gatcaccaat tccacaattc cacattcatc atcaatctgc aagctaatta
     1201 tacctgcttt ttccgaaaaa ataagtactt ttgcaagata tatccaaaaa tcatggaaaa
     1261 tacgaacaaa gatactgccg ttttactgtt acactgccct gacagacaag gtatattagc
     1321 ttctgtaaca gactttatca atgtcaacaa aggtaatatt atctatttag accaatatgt
     1381 tgacactata caaaaagcat tctttatgcg tgtagaatgg gaattggaaa gttttctaat
     1441 ccctaaagaa aaaattaaag aatactttga aacattgatg gctaaaaagt atagcatgga
     1501 tttccaatta tacttacatg cacaaaaacc cagaatggca atatttgtat caaaacaatc
     1561 acattgtttg tatgatttat tagcacatta tgaagcaggc gattggaatg tagagattcc
     1621 tctaatcata agtaatcatc ctgatatgga acatgttgct aaaaagttca acataccata
     1681 ctactgtttc cctattacca aagaaacaaa agaagaacaa gaagctaaag aattggcact
     1741 acttaaagaa aacaatatat cgtttttagt cttggcaaga tatatgcaag taatatcgcc
     1801 aaactttatt cgtgcatatc ctgaaaagat aataaacata catcactcat ttttgcctgc
     1861 ctttgtagga gcacgtcctt atcatgcagc atacgaacgc ggtgtaaaaa tcattggagc
     1921 aacaagtcat tacataactg aagagcttga tgccggacca attatcaaac aagatgtaac
     1981 aggcatatca cacaaggata ctgttgaaaa cttgattcgc aaaggtcagg atttggaaaa
     2041 aatagtatta tcacgtgcag tagaaaaaca tatcgaacat aaaatattgg tatataaaaa
     2101 taaaaccata gtatttggat gataatatga acatagccat tgtaaaatat aatgcaggca
     2161 atatttattc agtacaacac gcattaagac gtttgggtat cgaacctgta gtaactgacg
     2221 atattcaaat tttacgttct gcagataagg ttatatttcc cggggtcggt gaagcatcaa
     2281 ccacaatgtc gtatcttcaa aacaaaggat tagacaagct gatatgttca ctcacccaac
     2341 ctgtacttgg tatatgtttg gggatgcaac taatgtgcgc ttattcggaa gaaggcggaa
     2401 aaacagagag tttgggcata tttgatgctc ctgttaaatt gtttatccca caaaacagag
     2461 aaaaaattcc gcacatgggc tggaatcaga tatacaattt aaaatcgccc ttatttgccg
     2521 gtactaaaga aaacgattat gtgtattttg ttcatagtta ctatgttcct gtttgcaaag
     2581 ataccattgc aagttgtgat tacatagaag cttttagcgc agctctgcat aaaaacaatt
     2641 tttacgctac tcaattccac ccggaaaaaa gcggcagcat aggcagcaaa attttagagc
     2701 atttcttggc attatgatac aattaatacc ggcaatagat ataatagaag gcaaatgtgt
     2761 acgtttgtcg caaggcaagt acgatcaaaa gaaagtctat aacgaaaacg ccttggaagt
     2821 tgcaaaagaa tttgaagcac atggcatcac tcgtctgcat ttagttgatt tagacggagc
     2881 aaaagccaaa catattgtaa attataaagt cctcgaaaat atagcatcaa atacaaattt
     2941 atgtatagat tttggaggtg gcttaaaaac tgacgaagac ctcaaaattg cattcgaatg
     3001 tggagcatca atggttaccg gaggtagtat agccataaaa gatgcagcaa cattcagctc
     3061 atggataaat atctacggca aagacaagat catattggga gctgatgtaa aaggcgaaac
     3121 aatagcatta aatggttgga cagaagaaag cgattgccaa ctgatgccat ttttagacag
     3181 ctatatacaa aaaggaattg ataaagttat ttgcacagac atcgcaaaag atggtatgtt
     3241 agaaggtccg tcaattgatt tgtacaaaaa gattttatcc aagcataaaa atttatattt
     3301 aattgcaagt ggcggcgtta gcagcatgga agacatcgca gcattagagt ctgcgggctt
     3361 accggctgtt atattcggga aagccatata tgaaggacgc atcagcctaa aacaacttga
     3421 atcatcaata atacaaaaac atataataat atgctagcca aacgtatcat accttgttta
     3481 gatgttaaag acggaaaaac cgttaaaggc atcaattttg taaattttag agatgctgga
     3541 gaccctgttg agttaggtgc aaaatacagc gaagaaggag cagacgaatt ggtttttctc
     3601 gacattacag catcacacga aggtcgtaaa acctttgtcg acatggttaa acgtgtagca
     3661 gcaaatataa gtattccttt tacagtaggt ggcggaataa acgagttgtc agatgtagaa
     3721 cgtttactta atgcgggtgc agataaggtt tctgttaatt cagctgccat tcgcaatcct
     3781 caacttatca acgacatagc tcgtaatttc ggatcacaag tttgtgttgc tgcaattgat
     3841 gcaaaatacg atgatggtgt atggacctgt tatcttaacg gaggtcgaat cccgaccgaa
     3901 aggagtcttt ttgaatgggc aaaagaagca caagacagag gagccggtga gatattattt
     3961 accagcatga gccacgacgg cgttaaaaca ggctatccaa acgatgcctt agcaacattg
     4021 gccgacactc ttactatacc tgtaatcgca tcaggtggag ccggtacaaa agagcacttt
     4081 aaagatgctt ttttaatagg taaggcagat gcagcattag cagccagcgt attccacttc
     4141 ggagaaatta aaatacccga actaaaagaa tatttacgaa ataacaatat acctgtaaga
     4201 tgaaattaga ttttgcaaaa caaggcggac taattcccgc aattatccaa gacaatgtta
     4261 ctaacaaagt attgatgtta gggtacatga atgaagagtc cttagcaaaa actgaagaaa
     4321 ttggtaaagt aacttttttc agccgtagca aacaacgttt atggactaag ggcgaacaaa
     4381 gtggcaactt cttaaatgtt gtatcaatta aagaagattg cgacagtgat actctattaa
     4441 tcaaggttaa tccggaaggt cctgtatgcc ataccggcag cgatacttgc ttcgacgaag
     4501 taaaccaaat ggatgacttg atgttcttga aacatttgca agattacatt gacaaacgct
     4561 ataaagaaat gcctgaaggt tcatacacca catcattatt taaggatggt gtaaatcgta
     4621 tggcacaaaa ggttggagaa gaagccatcg aaactgttat tgaagctaca aatggtaaca
     4681 ataacagatt aatttacgaa gcatcagacc ttatatatca ccttatggta ttacttacat
     4741 caaaaggttt acgcatcgaa gacttagcaa tagatttaaa aaaacgtcat aaaaaatcaa
     4801 gcaatgaata ccctcatcaa gtataataat gttgacattt accaaaaaga gcagttagtt
     4861 cttaaaaatg tcaatctaga aatcggtaca ggcgagttct attaccttat aggtaaggta
     4921 ggttcaggga agagtagcct tttaaaaacc atgtacgctg aattgccaat agaaaatggc
     4981 gaagcaaaca tatttgaata caatcttatt gatattcgca ataaagatat tccacattta
     5041 cgcagacaaa taggaattat ctttcaagac tttcaattat taaatgacag aacggtaaaa
     5101 gagaatttat tgttcgtatt aagggctacc gactggaaag acaaggacga tatggataaa
     5161 cgtattgaag aagttttgtc tgaagttggt atgagtacca aaggatacaa aatgccacat
     5221 gagttatcag gtggtgaaca acaacgtata gttattgctc gtgctttact taataagcct
     5281 aaacttattt tggcagatga gcctaccgga aatcttgacc ctcaagccgg taaagattta
     5341 gttgaattat tgcaaaaaat atgtcattca gatagcggta caactattat tatggcaaca
     5401 cacaatctag gttggcttga ctcctatccc ggcaaagttc ttagcatcga gaaggatacg
     5461 ctaacagaat cgtagtaatc attattgtca gaactatgat ttttgtgatt aatggattac
     5521 aatgattaat agtaaatagc aaaattgcaa gttaagcaat aacgcggaac ttattttggg
     5581 caattttgta ggataagggc gatttaaata cttaaatact aaactacaaa aaattaaaaa
     5641 aataacaatg cttgcatttc tttgagatgc aagcatttgt tttgtattgg agattatta
//
LOCUS       DAOPID010000087         5685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_18678_length_5685_cov_4.651332, whole genome shotgun sequence.
ACCESSION   DAOPID010000087 DAOPID010000000
VERSION     DAOPID010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 5685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5685
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18678_length_5685_cov_4.651332"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="PK641_04105"
     CDS             complement(<1..479)
                     /locus_tag="PK641_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HPG55565.1"
                     /translation="MLNHKWIINELTEEQKKCKEELVKQLNISPIICTLLVQRGIYTY
                     EEAKKFFRPQLSQIHDPFLLNDMDKAVERLNLAMGKKEKILIYGDYDVDGTTAVALVY
                     KFLQQFYSSIDFYIPDRYEEGYGVSYKGIDYAAENGFSLIIVLDCGIKAIKKVKYAK"
     gene            602..1165
                     /locus_tag="PK641_04110"
     CDS             602..1165
                     /locus_tag="PK641_04110"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPG55566.1"
                     /translation="MSDEVKKTIEVLRAGGIILYPTDTVWGIGCDATNAEAVEKIYKI
                     KQRDDSKSMLVITDGMARIQSYVDEIPDIAWDLAELADKPITIIYPQAKNLAANLISD
                     DGSIGIRISSEKFSKQLCAAFRRPIVSTSANISGEPSPECFAEISDIIKDQMDYIVQY
                     RQDDTQKKQASSIIKLKKNGTFVILRH"
     gene            1171..2568
                     /locus_tag="PK641_04115"
     CDS             1171..2568
                     /locus_tag="PK641_04115"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPG55567.1"
                     /translation="MNVKYQLYKYLIADAISAFVTWVIFYVFRRIEVDLSTFSLNLFM
                     PQYNARILFPLVPIFWLFMYWLSGYYGEVCFKSRLSEFWTTLTSTLFGTLILFFTIVI
                     DDPVSDYTIFYTSYLLLFGMQFTFTYFTRLCITTITQKNMQKRRIGFNTLIIGVGEKA
                     VKLQSDLDSMPKSLGFNVVGFASFNSDSKVCVDSKSVLGNINDVENLLLNYKITVVII
                     AIDRVSESEIYDLMHILCGKSVKIKLIPSRYELLTGSVKLTNIYGIPMVDLTEVVMPE
                     WQKNVKRLTDIVISAIVLIVFSPVYAIIALKIFKKPIYKQERIGLHGKPFMMYKFRTM
                     VIDAENGEALLSSKDDPRIIKIGHFLRKYRLDELPQFFNVLKGDMSLVGPRPEREYFI
                     NQIVKDAPYYYMLSNVRPGLTSWGMVKFGYAQTVEQMIERANYDVLYLENMSIIVDCK
                     IAIYTIKTIITGKGI"
     gene            2570..4336
                     /locus_tag="PK641_04120"
     CDS             2570..4336
                     /locus_tag="PK641_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HPG55568.1"
                     /translation="MISNWYTKLLNWREKKIPTQRFVILLSFFVGIFTALSACLLKAG
                     IHGIQKLLTLNFNINSENFLYLLYPVIGILLSALFVKYIVKDDISHGVTRILYAISQR
                     KSILKSHNMWTSVVASSMTIGFGGSVGAEAPVVLTGSAIGSNLGRFFKMDQKTLMLMV
                     GCGAAGAISGIFKAPIAGVVFTIEVLMLDMTFTSIVPLLISSVTATAVSYFLMGDAVM
                     FSMQGEAFVLNRIPAYLMLGVVCGFASLYFTRGMNWLEDFFRKLKNFWSKLVIGGLIL
                     SALIFLLPPLYGEGYDSISALLNNNTNGLFDNSLFYNFKDNQYWLLFYMSLIIIFKIF
                     ASAATNAAGGCGGIFAPSLFVGCFVGFIVAIVLQLIGVPVPAQNFALAGMAGLMSGVM
                     HAPLTGIFLIAELSGGYSLFMPLMIVSVVSYCTIILFEPHSLYAMRLAKKGELITHHK
                     DKAVLSLLKTENVIETDLQVIRPEAMFGDLIKLISKSKRNIFPVVDESGVFLGVVTLE
                     EIRNIMFRSELYNRFNVQKLMVSPPATVSIDESMDAVMKKFEQTHAWNLPVLDGDKYV
                     GYVSKSKIFSAYRDVLVEFSSD"
     gene            complement(4337..5587)
                     /locus_tag="PK641_04125"
     CDS             complement(4337..5587)
                     /locus_tag="PK641_04125"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPG55569.1"
                     /translation="MRKIIFLTIIPLFALFTSCNDTNTGEPTEDPPKNDIKPSTFTHL
                     PIVRIIIDGGKNVSSNKSVYRTMTAEFDPNNMSPGDTIIAKGQIRGRGNSTWDMPKRP
                     YKIKLDEKAGIFGLNPAKKWILLANYADKTLMRNYLAFEMSEKLNMDYTPQHLFVELF
                     INGVHQGNYLLTDQIEIGNGRLEAVNLLEIDQRIYETQNKPWFKTSRFPVVIEEPTDI
                     TQAEKNQISGYFNNAERILDSNNYKDPKNGFRKYYDTKSVIKWLLINELFKNVDARTY
                     SSIYFHQRLTGEKLFMGPVWDFDLGAGNSGHSEVCQRATGWHVLYNAYIYRMYTDPDF
                     KQEMQVAWNTYKPVLLAVIMSIDSVKEYLKYSQEENFNIWPIFNDNEWYSVTNLPDNY
                     AGQVEFLKKYLLDRFDWMDSQINP"
BASE COUNT         1723 a          862 c         1066 g         2034 t
ORIGIN      
        1 tcttttgcgt atttaacttt ctttatggct ttaataccac agtcaagtac gataattaat
       61 gaaaaaccat tttcagctgc atagtcaatt cctttatatg atacgccata tccctcctcg
      121 tatctgtcgg gaatgtagaa atctatgctt gaataaaatt gttgtaaaaa cttgtaaacc
      181 aatgctaccg cggtagtacc atctacatca taatcgccgt aaataaggat tttttccttt
      241 ttacccattg ctaaatttaa tctctctact gctttatcca tgtcgttcaa taagaacgga
      301 tcatgaattt ggcttaactg aggacggaag aactttttag cttcttcgta agtgtaaata
      361 cctctttgta ccaacaatgt acagataata gggctgatgt tgagttgttt gactagttct
      421 tctttacatt ttttttgttc ttcagttaat tcattaataa tccatttgtg attaagcata
      481 tatgttttgt ttgtatatag caaggaataa tgtaaatttg ccaccgcaaa aatacaaacg
      541 gtaaaaatac atataatatt ttaaaaataa ggagatggat atggataatt atgaaaaaat
      601 aatatctgat gaggttaaga aaacaataga agttttacgc gccggcggaa ttatactata
      661 tcctaccgat actgtttggg gaattggatg tgatgcaaca aatgcagagg ctgttgaaaa
      721 aatatataaa ataaagcagc gtgatgattc caaatcaatg ttagttatta ctgatggaat
      781 ggccagaatt cagagttacg ttgacgaaat ccctgacata gcatgggatc tcgcagaact
      841 tgcagataaa ccgattacta taatttaccc tcaagcaaaa aatctagctg cgaatcttat
      901 ttctgatgat ggtagcattg gaataagaat aagttctgaa aagttttcaa agcaattatg
      961 tgctgcattt agacgtccaa ttgtttcgac ttctgcaaat attagcggtg aaccctcacc
     1021 tgaatgtttt gccgaaatat ccgacataat aaaagatcaa atggattata ttgttcaata
     1081 tcgtcaagac gatactcaaa aaaaacaggc atcatctata attaagttga aaaaaaatgg
     1141 tacttttgtc atccttagac actaatcgat ttgaatgtta aatatcaatt atataagtat
     1201 ctcatagctg atgcgatttc cgcatttgta acttgggtta ttttctatgt ttttagacga
     1261 atagaggttg acttatctac gttcagcctc aatttgttta tgcctcagta taatgctaga
     1321 atcctgttcc ctttagtacc aatattttgg ttgtttatgt attggctgtc aggatattac
     1381 ggtgaagtat gttttaaatc gaggttgtct gagttctgga caactcttac atcaacatta
     1441 ttcggtacat taatattatt ttttacaatt gtaattgatg atcctgtatc cgattatacc
     1501 atcttttaca catcatatct gcttctattt ggaatgcaat ttacatttac gtactttaca
     1561 cgtttatgca ttaccacaat tacgcagaaa aacatgcaaa aaaggcgaat aggatttaat
     1621 actctaataa ttggagtggg agaaaaagct gtaaagttgc aatctgattt agattctatg
     1681 cctaaatcac taggctttaa cgttgtaggt tttgcttctt tcaattctga cagtaaagtt
     1741 tgtgtggact caaaatcagt acttggtaat attaatgatg tagagaatct attgttgaat
     1801 tacaaaatca cagtagttat tattgcaata gatagagtaa gtgagtctga aatatatgat
     1861 ttgatgcata ttttgtgtgg caaatcggta aaaatcaaac ttataccaag tagatatgaa
     1921 ttgttgacag gtagtgttaa attgactaat atctatggca ttccaatggt tgatttaaca
     1981 gaggttgtta tgcctgaatg gcagaagaat gtaaaaagat taaccgatat tgtaatatcc
     2041 gccatagtac tgattgtgtt tagtcctgtg tatgcaataa tagcattaaa aattttcaaa
     2101 aaacctatat acaagcaaga acgtatcggc ttacatggta aaccatttat gatgtataag
     2161 tttagaacaa tggttattga tgctgaaaat ggggaggctt tgttatcatc aaaagatgat
     2221 ccgagaatta ttaagatagg gcattttttg aggaaataca gattagacga attacctcaa
     2281 ttttttaatg tacttaaggg tgatatgtct ttggtaggac ctcgtcccga gcgagaatat
     2341 tttattaatc aaatagtaaa agatgctcca tattattata tgttgagtaa tgttcgtccg
     2401 ggtctgactt cgtggggaat ggttaagttt ggatatgctc aaactgttga acaaatgatt
     2461 gagcgtgcaa attatgatgt tctttatttg gaaaatatgt caataattgt tgattgtaaa
     2521 attgcgatat atacaattaa gacaattatt acagggaaag ggatttaata tgatatcaaa
     2581 ttggtatacc aaattattga attggcgtga gaaaaaaatt ccaacacaac gctttgttat
     2641 tttactaagt ttttttgttg gtatttttac tgctctttct gcgtgcttgc ttaaggcagg
     2701 gattcatgga atacaaaaat tattgactct caatttcaat attaattccg agaattttct
     2761 gtacttatta tacccggtta taggtatttt gttgtctgca ttattcgtta aatatattgt
     2821 taaagacgac ataagtcatg gtgttactcg aatactatat gcaatctctc agcgtaaaag
     2881 tattctgaaa tcgcacaata tgtggacttc agttgtagca agttctatga caattggttt
     2941 tggtggatcg gtcggagctg aggctcctgt tgtacttaca ggttcggcaa tcggttcaaa
     3001 ccttggcagg ttctttaaaa tggaccagaa aacattgatg cttatggttg gttgtggagc
     3061 tgcaggtgca attagtggaa tttttaaagc tccaattgcc ggtgtagtat ttactatcga
     3121 agtattaatg ttggatatga cttttacctc gatagttcca ttgttaatat cctcagttac
     3181 tgctactgct gtatcttatt ttcttatggg tgacgcagtt atgttctcta tgcaaggtga
     3241 ggcatttgtg ttgaatcgca tccctgcata cttaatgtta ggtgttgttt gcggttttgc
     3301 atcattgtat tttacgcgcg gaatgaattg gttggaagat ttctttcgta aattaaaaaa
     3361 cttttggagc aaactggtta ttggtggttt gattttgagt gcattgatat ttttattgcc
     3421 tcccttatac ggtgagggtt acgattcgat atcagcttta cttaataata ataccaatgg
     3481 attgttcgat aatagtttgt tttacaattt taaagacaat caatactggt tgttgtttta
     3541 catgtctttg ataataatat ttaaaatatt tgcatctgcc gctacaaatg cagccggtgg
     3601 ttgtggtggt atttttgctc catctttgtt tgtgggttgt tttgtaggat ttattgtagc
     3661 aatagttttg caacttatag gtgtgccggt tccggctcag aattttgcat tggcaggaat
     3721 ggcaggattg atgtcgggag ttatgcatgc tccattaaca ggtatatttc taattgccga
     3781 actatcagga ggttattctc tgtttatgcc tttgatgatt gtttctgttg tgtcttattg
     3841 tacaattata ttgttcgagc ctcacagttt gtatgcaatg cgtttagcaa aaaaaggaga
     3901 gttgattacg catcataaag acaaagcagt tttaagtttg ctgaaaactg aaaatgttat
     3961 agaaacagat ttgcaagtta ttagaccgga ggcaatgttt ggtgatttga taaaattgat
     4021 ttcaaaatca aaacgtaata ttttccctgt agttgatgag tcaggggttt ttctcggtgt
     4081 agtaactctt gaagaaattc gtaatataat gttccgatcc gaattgtata atcgcttcaa
     4141 tgtacaaaaa cttatggttt cgccgccggc aacagtatct atagatgaat ctatggatgc
     4201 cgttatgaaa aaatttgagc aaactcatgc ttggaatcta cctgtgcttg acggagataa
     4261 atatgtaggc tatgtttcaa aatcgaaaat attcagcgca tatagagatg tgcttgtaga
     4321 attttcttct gattgattat gggttaatct gcgaatccat ccaatcaaag cgatcaagta
     4381 aatatttttt taaaaattca acttgtcctg cgtaattatc aggcagattt gttaccgaat
     4441 accattcatt gtcattaaag ataggccaaa tattaaaatt ctcttcttgt gagtatttta
     4501 agtattcttt tacagaatct atcgacataa ttacagctaa caaaacaggt ttgtaagtat
     4561 tccaagccac ctgcatttct tgtttaaaat caggatccgt atacattcga tatatgtagg
     4621 cattatataa aacatgccaa ccggttgctc tttgacatac ttcactatga cctgagttgc
     4681 ctgctcctaa atcaaaatcc caaacagggc ccataaataa tttttcgcct gtcaaccttt
     4741 gatgaaaata gatgctggaa tatgttcggg catcaacgtt tttaaataat tcgtttatca
     4801 ataaccattt tattacactt tttgtgtcat aatatttcct gaatccattt ttggggtctt
     4861 tataattatt tgaatctaaa atcctttcag cattattaaa ataccctgaa atctgatttt
     4921 tctcggcttg agtaatatct gtaggttctt ctatgactac agggaaacga gatgtcttaa
     4981 accaaggctt attttgagtt tcgtaaatgc gttggtctat ttctaaaaga ttgactgctt
     5041 caagtctgcc gtttcctatc tctatttgat ctgtaagaag ataatttccc tgatgcactc
     5101 cgtttataaa cagttcgaca aaaagatgct gtggagtata atccatattc agtttctcgc
     5161 tcatttcaaa ggcgagataa tttcgcatca aagttttgtc ggcataattg gcaagtagaa
     5221 tccatttttt tgccggatta agtccgaaaa tgcctgcttt ttcgtctaat tttattttgt
     5281 atggacgttt gggcatatcc caagtagaat ttcctcttcc tcttatttgg ccttttgcta
     5341 ttatagtatc accggggctc atgttgttgg ggtcaaattc ggctgtcata gttctgtaaa
     5401 cactcttatt tgaacttaca tttttgcctc cgtcaataat aatgcgtact ataggtagat
     5461 gagtgaaagt gcttggtttt atatcgtttt ttgggggatc ttccgtgggc tcaccggtat
     5521 ttgtatcgtt acatgatgta aagagtgcaa aaaggggtat tatagttaaa aatatgattt
     5581 ttctcataat attattatag gccgcaaacg atataaataa caaagatgag gttttttttg
     5641 ttaaatatca aagtttttgt tgcttttgta taaaaattag actta
//
LOCUS       DAOPID010000088         5668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_18758_length_5668_cov_3.882950, whole genome shotgun sequence.
ACCESSION   DAOPID010000088 DAOPID010000000
VERSION     DAOPID010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18758_length_5668_cov_3.882950"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1839
                     /locus_tag="PK641_04130"
     CDS             <1..1839
                     /locus_tag="PK641_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55570.1"
                     /translation="INAVATRLTCAVSSIQLEAQTDVQNVSYLWSNGSTDQSINVTTV
                     GMYSVTITNTDNGCSSTSSQVEITEDKTAPSASIISTSTELNCLTNSIDLTSSTDASN
                     ADYLWSNGATSKDINVTEVGTYSVQITNTDNSCATTSNAIEITENKKQPIVDINPKSG
                     SLTCDEPTLVLTSSTDAVTPQYLWSNGTTESTLTVSTGDNYYVTVTDASNGCYVQSET
                     VSVNDEKTYPSLTIVPENPVLTCTQTTVILEAQANNVTYLWSNSSTSSTIEVSVAQEY
                     SVTITDITTGCTNTASVVVTESTDKPNVQISSQETVLTCKTTSIVLTAEANNVTYLWN
                     DGSTDATLNVNEVGIYNVTVTDPTNGCQNSANSEEITENIEIPQVSITAPETEITCQN
                     QKVVLTAETQNDVDYLWDDNSSLETRDILAAGSNYWVEVTDKENGCVGKADAPIITDV
                     REMLDFDLQLSNEEVVLGNEATVEVNVTSGDYNNVIWQRNGEIFVPSDELQSTEKSYI
                     DVTYTITLEGDCNTISKEAIVKVLWPTAFTPYNTSSNLNRTFVTDMNPNFPIVVYTRY
                     GNKILESPNGWDGKCAGQMCMPGVYYYQVSLPNGEIKKGTIELHKE"
     gene            2054..3631
                     /locus_tag="PK641_04135"
     CDS             2054..3631
                     /locus_tag="PK641_04135"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HPG55571.1"
                     /translation="MKRGQIKSLCLVFAACLAITGCNNKQTEEKSENEPEVPVFVVDT
                     QNKRQVVDGIGSSLTESSAYVLACLSVKERQSLLEELFGENGANFPITRTHIGACDFS
                     VEGRYSLAEQNCDTALVSFSMFHDKEGFSTEKYPDIVDSTYDLYYLMKDVADIKAKQN
                     DKTYRVVATTWTAPAWMKDINDYYSKEKKTGGRLLKKYYQTYANYLLKYLEFYKSEGI
                     NCWALSPVNEPQGNDGSWESMHFTPQEESEFIGQYLGPTLEKAGYGDVKILGFDQNTF
                     EIGPWTAAIYGDSLSRKYTYGMAVHWYGSSFTPFPEVMDSIHNLYPDKAIIHTEGCID
                     NLGVEPWDAVIDKEGFVEKDWYKNDKFWWSKEATDWAYSTPFWSELHPKYVPVHRYAR
                     FIIEGMNNWMTGFIDWNVVLEPNGGRNHVGNHCGAPIMIDTKNKEIYYTPVYKVLKQL
                     SRNMRPGDTVIYVKQAEELAETVFVNAVQKPDGRIVVNILNTREEPLSFKLEIDDKDY
                     AVSVSANAVQTRIYPAK"
     gene            complement(3719..>5668)
                     /locus_tag="PK641_04140"
     CDS             complement(3719..>5668)
                     /locus_tag="PK641_04140"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HPG55572.1"
                     /translation="DRVLLNSPIQYLYGSVDSKGAGYTSCIYKDKLYLGTNQGLYVTQ
                     YPFKSNERKTQLSLVRKISGQVWNLSVINNTLFCCHNRGLYIINNQDGRPIDSRDGYW
                     LIKPMHKHSNMAIAGSYNGLYLLKNEFGQWRISNIIKGYTSSTRMFELDKNDNIWILS
                     TRGVERLILNDKLDSCTAKVMKKYPNNGYCSILPWEDEIIICSNGSCQMTDINSNLVD
                     SVDFFNEILDGEKLYSAVKKSDNKDVWFMYGDVLKVRKYDHNAMRYESKTTEIWDYPN
                     LYIGGFEHIQILNNTEAIVGCTSGFAMAKLNTVLHPNQGVWMKPTIRKLISTNQNDTI
                     IYGESYPKIKKDIEIKYKNNSLRFIIDGYDNYNEHVLYSYCLSPIESDYNKWIRSNTK
                     EYTNLSEGTYTLKIRTMSAASGQTAENSLEFTILPPWYRTWWAYLLYLIPIMYIIYLV
                     YKYFKHRVEKGRLLLERRKDSEMQKQQLKYAEESHEREKEILRLQNEQTEIELKSKSQ
                     ELANMMLSHLNKNEILTEIKKDLKKILGDLHGSNAEQISRKIIVLQGKITQNIEQEVD
                     WNKFEENFDLVHSQFIQKLMKKYPMLNKKERKLCIYVKMGLFTKEIAPLMNMSVRGVE
                     MLRFRMRKHMNLDRADDLEQLLEKI"
BASE COUNT         1873 a          876 c         1035 g         1884 t
ORIGIN      
        1 ataaatgctg ttgctactag attgacttgc gcagtatcaa gtattcagct agaggctcaa
       61 actgatgtgc aaaatgtaag ttatctatgg agtaacggca gcacagatca aagtattaat
      121 gttactacag tcggtatgta ttctgtaact ataacaaata ctgataatgg ttgttcttca
      181 acaagtagtc aagttgaaat tactgaggat aagactgctc caagtgcatc aataatttct
      241 acatctaccg aattaaattg tttgactaat tcaatagatt tgacatcatc gacagatgca
      301 tcaaatgcag attatttgtg gagtaatgga gctacgtcta aagatataaa tgttactgag
      361 gtaggaacat attcagttca gataacaaat acagataatt catgtgctac aacaagtaat
      421 gcaattgaaa taacagaaaa taagaaacag ccgattgtag atataaatcc aaaatcaggc
      481 tcgttaacat gtgatgaacc tacattggtt ttgacatcaa gtacagatgc tgtaacccct
      541 caatatttat ggagtaacgg aacaacagag tctacactta cggtaagtac tggtgataat
      601 tattatgtta ctgtaaccga tgccagcaac ggctgctatg ttcagtctga gacagtttct
      661 gtaaatgacg aaaaaactta tcctagctta acaatagtgc ctgaaaaccc tgtcttgaca
      721 tgtactcaaa caacagtaat acttgaagct caagctaata atgttacgta tttgtggagc
      781 aattcttcaa cttcaagtac tattgaagta agtgtggctc aggaatattc agttactata
      841 acagatatta caaccggatg tactaatact gcgtctgttg tagttacaga aagtactgat
      901 aaacctaatg ttcagataag ttcacaagaa actgttttaa catgtaaaac aacttcaatt
      961 gtgttgactg cagaagcaaa taatgtaaca tatttgtgga atgacggctc tactgatgca
     1021 acattaaatg taaacgaagt aggtatttat aatgttaccg ttacagatcc aactaatggt
     1081 tgtcaaaatt cggcaaatag tgaagaaatt actgagaata tagaaatccc gcaggtttct
     1141 attactgcac cggaaacaga aattacatgt cagaatcaaa aggttgtatt gactgcagaa
     1201 acgcaaaacg atgtcgatta tttgtgggat gataattcaa gtttagagac acgtgatatt
     1261 ctagcagcag gctcaaatta ttgggtagag gtaacggata aagaaaatgg ttgtgtagga
     1321 aaagcagatg ctcctattat tacagatgtt agggaaatgc ttgattttga tttacaatta
     1381 tcaaatgaag aggtagtatt aggaaacgaa gctactgtag aggtcaatgt tacatcaggt
     1441 gattataaca atgttatatg gcaacgcaat ggtgaaatat ttgttccgtc agatgaattg
     1501 caatcaacag agaaatctta tattgatgta acatatacaa taacattgga aggtgattgt
     1561 aatacgataa gcaaagaagc aatagtaaaa gtgctttggc cgacagcctt taccccttac
     1621 aacacaagta gtaatttgaa tcgtactttt gttactgata tgaatccgaa cttcccgatt
     1681 gttgtatata cccgttatgg aaataaaata ctcgaaagtc ctaatggttg ggatggaaaa
     1741 tgtgcagggc aaatgtgtat gcctggtgtg tattactatc aagtatcatt gccaaatggt
     1801 gaaattaaaa aaggtacaat agaattacat aaagaataat gtaatattct tcaagacaat
     1861 agcttagcaa gctgtcggaa tccatatttt aaaagattcc aacagcttgc tttgtaattt
     1921 gtcgtatgat tgagaaaatc aaatcaatat cttagaaagt ttttgtgaaa gtaaactgtt
     1981 tttgataatc aaagaaataa gataaatttg cactgctgta ctacaatatt aattttttta
     2041 atgagtatta attatgaaaa gaggtcaaat taaaagtctg tgtttggttt ttgctgcctg
     2101 ccttgctata acaggctgta ataataagca aacagaagaa aaatcggaga atgaaccaga
     2161 agttcctgtt tttgtagttg atacacaaaa caaacgtcaa gtagtagatg gcataggttc
     2221 ttctttgaca gaatcttcgg cttatgtatt ggcttgcttg tcggttaaag aacgtcagtc
     2281 tttgttagaa gaactgtttg gagaaaatgg tgcgaatttt cctattacaa gaacacatat
     2341 aggtgcttgc gatttttcgg ttgaaggacg ctattcttta gcagaacaaa attgcgatac
     2401 agctttagtt tctttttcga tgtttcatga taaggaaggt tttagtacag aaaaatatcc
     2461 tgacatagtt gatagtacat acgatttata ttatttaatg aaagatgtcg ctgacattaa
     2521 agcaaaacaa aatgataaaa catacagagt ggttgctact acatggacag cacctgcttg
     2581 gatgaaagat attaatgact attatagtaa agagaaaaaa acgggtggac gtttgttgaa
     2641 aaaatactac caaacttatg ctaattattt acttaaatac ttggagttct ataaatcaga
     2701 aggtattaat tgctgggctt tgtcacctgt aaacgaacca caaggaaatg acggaagttg
     2761 ggaaagtatg catttcactc ctcaagagga atctgagttt attggtcaat atttaggacc
     2821 tactcttgag aaagccggat atggtgatgt taaaatacta ggatttgatc aaaatacttt
     2881 tgagatagga ccatggaccg ctgcaatata tggtgattca ttatcgcgta aatatactta
     2941 cggaatggca gtgcattggt atggcagctc atttactccc ttccctgaag taatggactc
     3001 aattcataat ttatatcccg acaaagcaat tattcataca gaaggttgta tagataattt
     3061 aggagtagag ccttgggatg cagtcattga taaagaaggt tttgttgaaa aagactggta
     3121 taaaaatgat aaattctggt ggagtaaaga agcaactgat tgggcttata gtacaccttt
     3181 ctggtcggaa ttgcatccta aatatgtacc cgttcatcgt tatgcgcgtt ttatcataga
     3241 aggtatgaat aattggatga caggttttat tgattggaat gtagtactgg agcctaatgg
     3301 aggtcggaat cacgttggaa atcattgtgg tgcgccaatt atgattgata ctaaaaataa
     3361 agaaatatac tatacaccgg tatataaggt attaaaacaa ttaagtagaa atatgcgtcc
     3421 cggtgatact gttatttatg taaaacaagc cgaagaactt gctgagactg tatttgttaa
     3481 tgcagtacaa aaaccggatg gacgtatagt tgtcaatata ttgaatactc gtgaagaacc
     3541 attgtcattt aaactagaaa tagatgataa ggattatgcg gtttctgtgt ctgcaaatgc
     3601 tgtgcaaaca agaatctatc ctgcaaaata aatagattta tattttatag taaaggcggg
     3661 cttctaaata ttttagagac ccgcctttta ttgtttggat aatgtttgat aattttaatt
     3721 agatcttttc taacaattgt tctagatcgt ctgcacgatc aagattcatg tgcttacgca
     3781 ttctaaatct aagcatttcg acaccacgta ccgacatatt cataagagga gcaatctctt
     3841 ttgtaaacaa tcccattttt acataaatac ataatttgcg ttctttttta ttaagcatag
     3901 gatatttttt catgagcttc tgaataaatt gtgaatgaac taaatcgaag ttttcttcaa
     3961 atttgttcca atctacttct tgttcaatgt tttgtgttat tttaccttgt aaaacaatta
     4021 ttttccttga tatttgttct gcattactgc catgtaaatc gccaagaatc ttttttaaat
     4081 ccttctttat ttcggttaaa atttcattct tgttaagatg gctgagcatc atattagcca
     4141 attcttggct cttgctcttt aattctattt cagtttgctc gttctgtagg cgtaaaattt
     4201 ctttttcgcg ttcgtgcgat tcttctgcat atttaagctg ttgcttctgc atctcagaat
     4261 ctttccggcg ttctaataat aggcgtcctt tctccacgcg atgtttaaaa tatttgtata
     4321 ctaaatatat aatgtacata ataggaatta aatacaataa gtatgcccac catgttcgat
     4381 accaaggtgg tagtattgta aattccaaac tgttttctgc agtttgacct gatgctgcag
     4441 acatagtcct aatttttaga gtgtaagttc cctcacttaa gttagtgtat tctttagtgt
     4501 tactgcgtat ccatttatta taatcagatt caatagggga tagacaataa gagtataata
     4561 catgttcgtt ataattatca tatccgtcaa ttataaagcg taaagaattg tttttgtatt
     4621 taatttcaat gtctttttta attttaggat atgattcacc atatattata gtatcatttt
     4681 gattcgttga aattagtttt cttatagtag gcttcatcca tacaccttgg tttggatgaa
     4741 gcacagtatt gagcttagcc atggcaaatc cggaggtgca tccaacaata gcttctgtat
     4801 tgttaagtat ttgaatatgc tcaaaacctc cgatataaag attcggataa tcccatattt
     4861 ctgtagtttt tgattcgtat cgcatagcgt tgtgatcgta ttttctgact ttcaatacgt
     4921 ctccatacat aaaccaaaca tctttattgt ctgatttttt tacagcagaa taaagttttt
     4981 ctccatcaag tatttcattg aaaaaatcta cagaatcaac caaattacta ttaatgtcgg
     5041 tcatttggca gctaccattg ctacaaataa taatttcgtc ttcccatggt aaaattgagc
     5101 aatagccatt attaggatac tttttcatta ctttcgctgt gcaagaatca agtttatcat
     5161 tcaaaatcaa gcgttctaca cctcttgttg ataaaatcca gatgttatca ttcttgtcta
     5221 attcaaacat acgtgttgat gaagtatatc ctttaataat attacttatg cgccattgac
     5281 cgaattcgtt tttgagtagg tataaaccat tgtaagatcc ggcaatagcc atattggagt
     5341 gcttatgcat aggttttatt aaccaatatc cgtcacgaga atcaataggc cttccatcct
     5401 gattattaat aatgtataag cctctgttat gacaacaaaa taaagtattg tttattactg
     5461 ataaattcca tacctggccg gaaatctttc ttactagtga taattgagtt tttctctcat
     5521 tacttttaaa aggatattgc gtaacatata gaccttggtt agtgcctaga tatagtttgt
     5581 ctttatatat acatgatgta taaccggctc ctttagaatc tacgcttccg tataaatatt
     5641 gtataggaga atttagtaga acacggtc
//
LOCUS       DAOPID010000089         5560 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_19300_length_5560_cov_3.952407, whole genome shotgun sequence.
ACCESSION   DAOPID010000089 DAOPID010000000
VERSION     DAOPID010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19300_length_5560_cov_3.952407"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PK641_04145"
     CDS             <1..180
                     /locus_tag="PK641_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55573.1"
                     /translation="ITVFNSMNMFWFAYIVVLILSVTYTCKITYKQSINIVLCSYGFV
                     SMLMIVFTCLFMMLQ"
     gene            177..632
                     /locus_tag="PK641_04150"
     CDS             177..632
                     /locus_tag="PK641_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPG55574.1"
                     /translation="MMKRNIFIAFVLLCLVGASIMLLIYHDRNRESYNFIQGLAYGLN
                     ISPNDTLIVSFFHPECDICHSMFPQYKEHQTKCSIILICYESDSVIQQTATTYEWNHY
                     PNIHLVSDSTFHCSNHLLVNSCPSTFTIVPKSEQVKRKIGYTNFKKILP"
     gene            697..2790
                     /locus_tag="PK641_04155"
     CDS             697..2790
                     /locus_tag="PK641_04155"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HPG55575.1"
                     /translation="MLPYLTYKIKRAGVLQQDSSDCGAACIATLLRFYGTHVNFENTR
                     EHCGTQGNGGNLLGLYQAAQHFGFKSGGAQGTVSDLKEIDNPSILHFIMPNGLQHFVI
                     FFTFHKNRFFLFDPAQGFLKMDEQTLSTLWSGNCLLIKGFAPLSDSLKTHKMQKLQWF
                     WQYLKPDKSILFISIIMGSICSVMGLGISLFSQKLIDNILPKQNTYILIVALCALFII
                     LVLQSISMYVKDHILLKQGSEFSTRLLSAFYKRFLALPLSFFENRKNGDLVARLNDCN
                     RIQKNLSGLFGSSLAGDLLIALSASILLGIYHYSLPIILMLGFICMLIVSYYATPSIT
                     KSQQQVMANHAICESNFMHTVEGIRCIKNENLSNSFTLQTNNIYTKFQQSIFQLGIIN
                     IKLRLFYQITGIIMVIITLLIGGFLVIKSQLTAGELIACLSLCSLVSPTLISLSLIPI
                     PISEATVAFDRLYESSNIALNNSNSVDFTNPITQIDIENLGFAYRAGKPILQNLNYHF
                     GIGKLYCINGQCGRGKTTLCKLLEKSYTSYSGNILINGEHELQKINTFAWKNKIGVIS
                     QEPFLFNATIFENIALHVNSQEEIQNALRICEEYHIVERFKNLSQGIFSTIGENGIQL
                     SGGEKQLVALARILIKNPDVLILDEPTAAMDQDLENFTWNTFIKLSKEKIVCVVTHKK
                     HILEQYKENINTLIL"
     gene            2826..3482
                     /locus_tag="PK641_04160"
     CDS             2826..3482
                     /locus_tag="PK641_04160"
                     /inference="COORDINATES: protein motif:HMM:NF039827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5687 family protein"
                     /protein_id="HPG55576.1"
                     /translation="MKRSYFFEDQIIGIVIKSVVAIWLLFSICASGAMLPMAIQEQIP
                     DVNLQSLFFYLLAGLCCIDLLIKYIKKNSFYQKMHPYLLLPIPKYKLFGIALCAEIVS
                     PWNWWMFVFCLPYLAQVYSIALCLSLLFLVWVLAIFNSLLVRFVKSLLNWITLQLLVC
                     ISFAIAYGSIIHFRLADAIYVFILSYPFVPFTASILLLLGCLYLFRKLSQKELYNSLS
                     "
     gene            3507..4205
                     /locus_tag="PK641_04165"
     CDS             3507..4205
                     /locus_tag="PK641_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007484322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG55577.1"
                     /translation="MITIKDLSKAFNQQVAVNISDLHIGKNELVGLVGNNGAGKTTLL
                     RLILDLYQADKGYVLSKGINVAQSEDWKIYTGAFIDSGFLIDFLYPNEYFDFIGRLHQ
                     FSKEEIKERLSQFEKFASGEIFGQKKYIRNFSAGNRQKIGIIGAMFTHPEILLLDEPF
                     NFLDPSSQNEMKRLLAYYQKEYGCTLLISSHNLEHITDICSRVILLEKGLIIKDLLGN
                     YSETAQELQTYFLQ"
     gene            4219..4881
                     /locus_tag="PK641_04170"
     CDS             4219..4881
                     /locus_tag="PK641_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG55578.1"
                     /translation="MIQIHQLHKTYTANENLHVLCGIDIDIKQGEFVSIMGASGSGKS
                     TLLNIIGLLDQFDKGSYLLDNEEMFQIDEDAAADIRLHKIGFIFQSFNLIPYKTALDN
                     ISLPLFYQNINKKERDNLAIELLKSMNLEKWKLHYPSELSGGQKQRVAIARAIITRPK
                     VILADEPTGALDSYTSKDILLLLRQINKQYGSTIVMVTHDPVIAQKADRIIRINDGIV
                     EC"
     gene            4988..>5560
                     /locus_tag="PK641_04175"
     CDS             4988..>5560
                     /locus_tag="PK641_04175"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPG55579.1"
                     /translation="MEFKILPYITHKRLLNMALIAADYLESVCTKKVILKTKPYAASI
                     EPANYCNLNCPQCPTGNGSIKKVPLCLSIENFKKIIKPLLPELTYLNLYFQGEPFLND
                     DLAKMIEYTHKERVFTCVSTNGHFLSKQICEELKKAKLDKLIICLDGATKDTYETYRK
                     GGDFNAVIEGIRNSVKAKLPVEVQCLLLKSN"
BASE COUNT         1895 a          932 c          896 g         1837 t
ORIGIN      
        1 attactgtat ttaacagcat gaatatgttt tggtttgcct acattgtagt acttatactt
       61 tctgttacat acacttgtaa aataacgtac aaacaaagta ttaacattgt actttgcagc
      121 tatggttttg tttcgatgct gatgattgtt ttcacttgtc tttttatgat gctgcaatga
      181 tgaaaagaaa tatatttatt gcattcgtat tactttgttt agtaggagca tctattatgc
      241 tcttgattta tcatgacagg aatagagaaa gttacaattt tattcaaggt ctggcatacg
      301 gtttaaatat ttctcctaat gacactctaa tcgttagctt ctttcatcca gaatgtgata
      361 tatgccatag tatgtttcct caatacaaag aacatcagac aaaatgttca attatactta
      421 tttgttacga atcagattct gttatccaac aaacagcaac aacttatgaa tggaatcatt
      481 atccgaacat tcatttggta tccgattcga cttttcattg ttccaaccat ttacttgtaa
      541 attcctgtcc aagcacattt acgatagttc ctaaatcgga acaagtaaaa cggaaaatag
      601 gatataccaa ttttaaaaag attctaccgt aaattaatcc attatcctaa agacaatatt
      661 tacaaaatat tataccattc aaaattaatc gtttaaatgt taccttatct aacttataaa
      721 attaaacgtg ccggtgtttt acaacaagat tcgtccgatt gtggtgctgc ttgcatagcc
      781 actcttttgc gattttatgg tacacacgtt aattttgaga atacccgaga acattgtggg
      841 acacaaggaa acggaggaaa cttattggga ttgtaccaag cagcccaaca ttttggattt
      901 aaaagtggag gagctcaagg tactgtatct gatttaaaag aaatagacaa tccaagcata
      961 ttacacttta ttatgccaaa cggattacaa cattttgtaa ttttctttac gtttcataag
     1021 aatcgctttt ttttattcga tcctgcacaa ggatttctca agatggatga acaaacatta
     1081 tctacattat ggagcggaaa ctgtttgctg attaaaggat ttgcaccttt gtccgattct
     1141 cttaaaacgc ataaaatgca aaaattgcaa tggttttggc aatatttaaa accagataaa
     1201 tccattttat ttatcagcat cattatggga tcgatttgtt ctgttatggg attaggcata
     1261 tcgctatttt cgcaaaaact cattgataat attttgccta aacaaaatac ctatattcta
     1321 atagtcgctt tgtgtgcctt atttattatt ctagttttac aatccataag tatgtatgta
     1381 aaagaccaca ttctactaaa acaaggatct gaattttcaa ctcgattgtt atctgctttc
     1441 tataaacgct ttttagcatt acccctttct ttttttgaaa ataggaaaaa tggtgattta
     1501 gttgctcgtt taaatgattg taatcgcata caaaaaaatc tatccggctt atttggcagt
     1561 tctttagcag gagatctact catagcttta tcggcaagca ttctcctcgg tatttatcat
     1621 tatagtttgc caattatttt gatgttaggg tttatctgta tgttaattgt atcgtactac
     1681 gcaactccgt ctattacaaa atcgcaacaa caagtaatgg ctaatcatgc catttgcgaa
     1741 tcaaatttta tgcatactgt tgaaggaata agatgtatca aaaatgaaaa tttatcaaac
     1801 tcctttactt tacaaacaaa taacatttat acaaaatttc aacaaagcat ttttcagtta
     1861 ggtattatca atattaaatt gcgattattt tatcagatta ccggcattat catggtaata
     1921 atcactttac ttattggtgg ttttttggtc attaagagtc aactaactgc tggagaatta
     1981 atagcttgct taagtttatg ttctttggta agtcctacac taataagcct ctcattaatt
     2041 cccattccca taagtgaagc aacagtagcc tttgatcgtt tgtacgaaag ttcaaatata
     2101 gccttaaata atagcaattc ggttgatttt accaatccta ttacacaaat agacatcgaa
     2161 aatcttggct tcgcttatcg ggcaggaaaa cctattttac aaaatcttaa ctatcatttt
     2221 ggaatcggta aattatattg cattaatgga caatgtggtc gtggaaaaac gacactctgt
     2281 aaattattag aaaagtcata tacgtcatat tctggcaata ttttaattaa tggcgagcat
     2341 gagttgcaaa aaattaatac ttttgcatgg aaaaataaaa taggagtaat aagccaagaa
     2401 ccatttttgt ttaatgcaac tatatttgaa aacattgcac tacatgtcaa ttctcaggaa
     2461 gagatacaaa acgcccttcg aatttgcgaa gaataccata ttgtagaacg ttttaagaat
     2521 ctatcacaag gcattttttc tacaattgga gagaatggaa ttcagctctc tggtggagaa
     2581 aaacaattag tggcactcgc tcgaatacta atcaaaaatc cagatgttct catattagat
     2641 gaacctacag ctgcaatgga tcaagatttg gaaaatttca catggaacac gtttataaaa
     2701 ctttctaaag aaaagattgt atgcgttgta acccataaaa aacatatact tgaacaatac
     2761 aaagaaaata ttaatacctt gatactgtaa ccatgcgcga gttgttatct cattacctgc
     2821 tttcgatgaa acgatcttac ttttttgaag atcaaataat aggcattgtc attaagagcg
     2881 ttgtggctat atggctactt ttttcgattt gtgcgagcgg tgctatgcta cctatggcta
     2941 tacaagaaca aatacctgat gtcaatttac aatccttatt tttttattta ttggcaggtt
     3001 tgtgttgcat tgatttgctc ataaaataca ttaaaaagaa ttcgttttat caaaagatgc
     3061 atccgtattt attgcttcca atacctaaat ataaattatt tggtattgcg ttgtgcgccg
     3121 aaattgtttc gccttggaac tggtggatgt ttgtattttg tttgccatat ttggcgcagg
     3181 tatattctat cgctttgtgt ttatccttac tgtttttggt ctgggtatta gccatattca
     3241 atagtttgct tgtgcgtttc gtaaagagtt tacttaattg gattacactc caattattag
     3301 tttgcatttc atttgcaatt gcttatggca gtataataca tttccgtttg gcagatgcaa
     3361 tatatgtttt tatactatcc tacccatttg taccctttac ggcatctata ctattgttgt
     3421 taggatgcct ttatttgttc cgaaaactta gtcaaaaaga attatataac agtttgtcgt
     3481 aaaatataaa tataaacaat aataatatga ttacaattaa agatcttagt aaagcattta
     3541 accaacaagt tgctgtaaat atttccgatt tgcatatcgg aaaaaatgaa ttagtaggtt
     3601 tagtgggtaa caacggtgca ggtaaaacca cactattgcg tcttatttta gacttgtatc
     3661 aggcagataa gggttatgta ctaagcaaag gtataaacgt agcacaatcg gaagattgga
     3721 agatttacac aggtgcattt atcgacagtg gttttttaat tgatttcttg tatcctaacg
     3781 agtacttcga ttttatcggc agactgcatc aatttagcaa agaagaaata aaagaacgtt
     3841 tgagccaatt tgaaaagttt gccagtggtg aaattttcgg acaaaaaaaa tatattcgta
     3901 acttttcggc agggaacaga caaaaaatag gtatcattgg agctatgttt acacatccag
     3961 agattttatt gctggacgaa ccatttaatt tcttggatcc gtcatcacaa aacgaaatga
     4021 aacgactgct cgcctattat caaaaagaat atggttgcac gcttttaata tcaagccaca
     4081 atttggaaca tattaccgac atctgttcac gcgtaatttt actcgaaaaa ggtttaataa
     4141 tcaaagatct tttgggcaac tactcagaaa ccgctcaaga actacaaact tattttttac
     4201 agtaaactaa tattataaat gatacaaatt catcaattac acaaaaccta taccgccaac
     4261 gaaaacctac acgttttgtg cggtatagat attgacatta aacaaggcga atttgtatcc
     4321 attatgggag catctggttc cggtaaatca actttgctaa atattattgg tttattggat
     4381 caatttgaca agggaagtta tttattagac aacgaagaaa tgtttcaaat tgatgaagac
     4441 gcagctgccg acatccgttt acacaaaata ggatttattt ttcaatcatt caatctcatt
     4501 ccgtacaaaa cagctttgga caatatcagc cttcctcttt tttatcaaaa tataaataaa
     4561 aaagaaaggg ataatttagc tattgaatta ctaaaatcca tgaatcttga aaaatggaaa
     4621 ttgcattatc caagtgaact ttcaggtgga caaaagcaac gagtcgctat agctcgcgca
     4681 attattacta gacccaaagt aattttggct gatgaaccca ctggtgcatt agatagctac
     4741 acatcaaaag acatccttct attactaaga caaataaaca aacaatacgg tagtaccatt
     4801 gtaatggtaa cgcacgaccc tgtcatcgca caaaaagccg accgaataat tcgcataaac
     4861 gacggaatag ttgagtgttg attaattaat tggaaagtgc acatattagt ttgcgataaa
     4921 aattcaagaa tattttaacc ctttttggct tgcgtagtcg gatgtaaatg ctcatttttg
     4981 catctaaatg gaatttaaaa ttttaccata tatcacacac aaaaggctat tgaacatggc
     5041 tttgattgca gctgattatt tagaatcggt atgcacaaaa aaagtaatac ttaaaacaaa
     5101 accttacgct gcgtcaatag agcctgccaa ttattgcaat ctcaattgcc cacaatgccc
     5161 tacagggaat ggctcaataa aaaaagttcc attatgcctt tcaatcgaaa actttaaaaa
     5221 aataatcaag cctttactac ctgaacttac atacctaaat ttgtattttc aaggagaacc
     5281 atttcttaat gatgacctgg caaaaatgat agaatacaca cacaaagaaa gagtatttac
     5341 ctgcgtatca actaatggtc atttcttatc aaaacaaatt tgcgaggaac taaaaaaagc
     5401 aaagctggac aaactgataa tatgcttaga cggtgccaca aaagacacat acgaaacata
     5461 tcgcaaagga ggagacttta atgcagtgat tgaaggaata agaaactctg taaaagcaaa
     5521 attacctgtt gaagtgcaat gcttattact aaaaagcaac
//
LOCUS       DAOPID010000090         5513 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_19521_length_5513_cov_3.676988, whole genome shotgun sequence.
ACCESSION   DAOPID010000090 DAOPID010000000
VERSION     DAOPID010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19521_length_5513_cov_3.676988"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /gene="dnaA"
                     /locus_tag="PK641_04180"
     CDS             complement(<1..1049)
                     /gene="dnaA"
                     /locus_tag="PK641_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPG55580.1"
                     /translation="MSADFSQLWDQCLEVIKDNVGEVGYKTWFEPIVPLQLVNDSFTI
                     QVPSHFFREYLEEKYADLMRSTIFRVFGPNTKLFYRVILDKDNKRGGSTDIPCSNQNA
                     ERKRVGISEFDSQLNYNYNFGNFIEGDCNKLPRTAGINIASQPGKAVFNPLLIYGKSG
                     VGKTHLANAIGLKTKELHPQKKVLYVSANLFQLQYTEASRNNQVNDFLNFYQMVDMLI
                     IDDIQEFGEGKKTSTQNTFFHIFNHLHQSGKQLILTCDKKPAELEGLEERLLTRFKWG
                     LTAEVEGPDFETRKAILKAKVEADGLKIPENVITYIAENVTDNIRNLEGTIISLIAQS
                     TMNQKTINIDLAKKVV"
     gene            complement(1362..2282)
                     /locus_tag="PK641_04185"
     CDS             complement(1362..2282)
                     /locus_tag="PK641_04185"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPG55581.1"
                     /translation="MGEKKKKFLTRETKIGIAVVVAIGMLYFGINYLKGINVFKPNTY
                     FYVQYDRVDGIVKTTHVLINGYKIGHVSDIYFDYTKEAPITLEITVDKHLVVPKGTIA
                     QVYETGLMGDKAIQLKLGNGTELCQTGDTLQGEVVGGMIAEIVQSLMPPIERLVPQLD
                     STILAIRKVAEDENIRKTLANAEQATKELKLASVKLNNIMNNDVPTIMTDVKTTTSKL
                     SKISTDIAEMDLKATIEKVNATLANLQFASDKFKSKDNTLGLLLNDKQLYLHIDSTAN
                     SANQLLIDLKANPKRYVHFSVFGSKDKAKK"
     gene            complement(2289..3476)
                     /locus_tag="PK641_04190"
     CDS             complement(2289..3476)
                     /locus_tag="PK641_04190"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPG55582.1"
                     /translation="MKHIKTMSLKHIITISLALCLFGYINAAPFTIVIDAGHGGHDAG
                     AIGSFSKEKNINLRHALLLGDMLKEKNPEIKVIYTRDNDVFVDLNERARIANRAKADL
                     FVSIHTNAAKNRSANGVETFTLGVSRNKENMEVAMLENSVILLEDDYEKNYQGFDPNS
                     TDSYIMFEFMKDQYMDRSITCADMVQTRLVNASQRLDRGVRQAGFLVLRATTMPSILV
                     ELGFISNREEEKSLNENKNQIALATALYKAIIAYKHDLEKKNGTGINTPAVPETPEPV
                     IKDTIVVKQIEEEKTAETAPISKVDNNKYEDIVFMVQIFSSKNKYKLNDAIFKGIKIT
                     ETKKEGNLYKYIVGKSTNLDAIIELRKNVSKEFPDAFIIAYKKGVKIPTKDAITEYQT
                     QSK"
     gene            3655..>5513
                     /locus_tag="PK641_04195"
     CDS             3655..>5513
                     /locus_tag="PK641_04195"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HPG55583.1"
                     /translation="MLFAFSYALSAYSQQDSSMLVPVVPLDTLTISLPLDSVASKNKN
                     SSEAIKKRKPKKTTQSITSDIAYNAKDSIVLIGNHTALLYGEAQVIYNDIELKADYIR
                     LRLDSNLVYATGVPDSTGENMKGKPVFKDGKESYEAKAMKYNFNSKKGFIWGTVTEQG
                     EGYVTSAKTKKISEDEYCLQHGKYTTCDQHDHPHFYLALTKAKMKQGKYIVSGPAYLV
                     VEDIPFPLAVPFGYFPVNQKYSSGIIFPTFGEEQTRGFYAKGMGYYFAINDYIDLALT
                     ADVYTKGSWSLALASSYKWRYKFGGSLNLSFIRNTYGDPNTPDYRSSNDFRVMWSHTQ
                     DQKMSPYTSFSASVNFSTSSYEQNNVDSYYNPALLSQNTKSSTITLTQTFPESPFTLS
                     LSMYVNQRTSDSTINLNLPSITLNMSRVCPFKRKSSVGKEKWYEKIAITYNLALSNSI
                     TAKESKFMQTNFLRDWKNGIKNEIPISASFTLFKYLNLNVGLNNSLRWYFSKVNQHWE
                     GDYSGAAVSDTTYGFYNLYKCSASVGLQTQLFGFYKIKSKPGKNAPIFRHKIVPSVSF
                     SIAPDFGAPGWDYWGTYDRPASDGSIKTVYYDKFAGGIYGGSPSRGASGSVSFT"
BASE COUNT         1685 a          917 c          918 g         1993 t
ORIGIN      
        1 acaaccttct tagccaaatc tatattgatg gttttttggt tcatggtaga ttgagctata
       61 agagatatga ttgtcccttc caaatttctg atattgtcag ttacattttc tgcaatgtaa
      121 gtaataacat tttcaggaat ttttaatcca tcggcttcaa cttttgcttt tagtattgct
      181 ttacgtgttt caaagtcagg accttcaacc tctgctgtaa gaccccattt aaaacgagta
      241 aggagacgtt cttccaatcc ttccaactca gccggtttct tatcacaagt caggattaat
      301 tgcttacctg attgatgtaa atgattaaat atatggaaga aagtattttg agtgctcgtc
      361 tttttgcctt ctccaaattc ctgaatatca tcaattataa gcatatcaac catttggtaa
      421 aaatttaaga aatcgtttac ttgattgttg cgtgatgctt cagtatattg caattgaaat
      481 aggtttgcag atacatacaa tactttcttc tgagggtgca attcctttgt tttaagacca
      541 attgcattag cgaggtgagt tttaccaaca cctgatttcc cgtaaatcag aagaggatta
      601 aatacagcct tacccggttg acttgcaatg ttaatgccgg cagtacgagg caatttattg
      661 caatctccct caataaaatt accaaagttg taattgtaat taagttgaga gtcgaactca
      721 gaaataccta cacgtttgcg ttctgcattt tggttactgc atggaatatc tgtagaacct
      781 ccacgtttat tatccttatc caaaataact ctatagaaca attttgtatt aggcccaaaa
      841 actctgaata ttgttgagcg cattaggtct gcatatttct cttccaagta ttcgcgaaag
      901 aaatggcttg gtacttgtat tgtaaacgaa tcgttcacta attgtagcgg aacaataggt
      961 tcaaaccaag ttttataacc gacctctcca acgttgtctt taattacttc aagacattgg
     1021 tcccataatt gtgaaaaatc agcgctcatt tttgtgtaat tattttaata attcataatt
     1081 cattgtttgg caaatatcgt tttttatttc ttgaaaaaaa aatgtgtttt tgtttgtttt
     1141 tttcaaaaca actgcaatgt tttgtgtctt aggtaattat actaacgtat tgatatacag
     1201 actattataa actaagattt gataaaacta ttgacaacca atgaattagc gtaatactta
     1261 tttattataa aaaatcgggg taaaaatagg gaaatcgtta tgtttcaacg gtttccctat
     1321 tattgttaaa atattgttaa aatagtaatt ttattttcgt cttacttttt ggctttgtct
     1381 ttacttccaa aaacagaaaa atgtacataa cgtttaggat ttgcctttaa atctataagt
     1441 aattgattag cactatttgc tgttgaatct atatgtaaat acaattgttt gtcgttcaat
     1501 agcaatccta gagtattgtc tttgctctta aatttatctg aagcgaactg caaattagct
     1561 aatgtagcat taaccttttc aatagttgct tttaagtcca tttctgctat atcagtacta
     1621 atcttagata atttagaagt tgttgttttg acatcagtca ttattgttgg tacatcatta
     1681 ttcatgatgt tattgagttt aaccgatgca agttttaatt cttttgtagc ttgctctgca
     1741 tttgccaaag ttttgcgtat attttcatct tcagcaactt tgcgtatagc taatattgta
     1801 gagtctagtt gtggaaccaa tctttctatt ggcggcataa gcgattgtac aatttccgca
     1861 atcatacctc caacaacttc gccttgcaaa gtgtcgcctg tttgacatag ttcagtgcca
     1921 ttgcctaact ttaattgtat agctttgtct cccattaatc cggtttcata aacttgtgca
     1981 attgttccct tgggaactac aagatgtttg tcaactgtta tttccaatgt tattggagct
     2041 tcctttgtat agtcaaaata tatatcacta acatgaccta ttttatatcc gttgatgagt
     2101 acatgtgtgg tttttactat tccgtcaact ctatcatatt gtacatagaa ataggtattc
     2161 ggtttaaata cattaattcc ttttagatag ttgataccga aatatagcat tcctattgca
     2221 acaacaacag caatacctat ttttgtttca cgtgttaaaa acttcttttt tttctcgccc
     2281 atagtgattt atttggattg tgtttgatat tctgttatcg catctttagt aggtatctta
     2341 accccctttt tatatgcaat aataaatgcg tcaggaaatt ctttgcttac atttttacgt
     2401 agttcaatta ttgcgtctaa gttagtcgat ttccctacta tatatttata tagattgcct
     2461 tctttttttg tttcggttat tttgatgcct ttaaaaatag cgtcattcaa tttgtattta
     2521 tttttagaag aaaaaatctg aaccataaaa acaatatcct catatttgtt attgtcaact
     2581 tttgatattg gtgctgtttc tgcagttttt tcttcttcta tttgtttcac aacaatggta
     2641 tctttaataa caggttccgg agtttcagga acagcgggag tatttatacc ggttccgttt
     2701 ttcttttcca agtcgtgttt atatgctata attgccttat ataatgctgt agccaatgca
     2761 atttgatttt tattttcatt tagagatttt tcttcttctc tgtttgaaat aaatcctaat
     2821 tcgaccaaaa ttgatggcat tgtggtggct cttaaaacca agaaaccggc ttgacgaacg
     2881 cctctgtcta atctttgact tgcattaacc agtcttgttt gaaccatgtc agcacaagtg
     2941 atacttctat ccatatactg atctttcata aattcaaaca taatgtacga atcggttgaa
     3001 ttagggtcaa aaccttgata attcttttcg taatcgtctt ctaataagat aactgagttc
     3061 tctaacattg caacttccat gttctcttta ttacgagata cacctaaagt aaatgtttca
     3121 actccattag cgcttctgtt ttttgctgca ttggtatgaa tagaaacaaa caaatcggct
     3181 tttgcacgat ttgcaattct agctctttca tttaaatcaa caaaaacatc attgtctctt
     3241 gtataaataa cttttatttc aggatttttt tctttcaaca tgtctccaag taggagagca
     3301 tgtcgtaaat taatattttt ttcttttgaa aatgatccta tggcacctgc atcatgtcca
     3361 ccatgtccgg catcaattac tattgtaaat ggcgctgcat tgatataacc aaacaagcag
     3421 agtgcaagtg aaattgtaat tatgtgtttt aatgacattg ttttgatatg tttcatgcgc
     3481 aaaagtagtg atttttttac taatgaggct tgatgtatga gcaaatatgt agcaaaaaat
     3541 attaaaaaat cagatacgtc tttatgttac ccttaaaatg tgtaactttg cggtttgttt
     3601 atatacacta ttctataaga ctctttgagg caaggttttc aaatactttt gagcgtgttg
     3661 tttgcatttt catatgcatt gtcagcatat tctcagcaag acagttcgat gttagtgcct
     3721 gttgtaccat tggatacact tactatttcg ttgcctttag attctgtcgc ttctaaaaat
     3781 aaaaattcaa gtgaggcgat aaagaaaaga aaacccaaaa aaaccactca atctattaca
     3841 tctgatattg cttataatgc aaaagattca attgttctaa taggtaacca tactgcatta
     3901 ctatatggtg aagcccaagt aatttataat gatatagaat taaaagctga ttatataaga
     3961 cttcgtttag acagtaattt ggtttatgct acgggagttc ctgattctac gggtgaaaat
     4021 atgaaaggta agcctgtttt taaagatggt aaagagtctt acgaagcaaa ggcaatgaag
     4081 tataatttta attcaaaaaa aggttttata tggggaactg taacagaaca aggtgaaggc
     4141 tatgttacaa gtgccaaaac aaagaaaatt tctgaagacg aatattgctt acagcatggt
     4201 aagtacacaa cttgcgatca acatgatcat ccgcattttt accttgcatt aaccaaggca
     4261 aaaatgaaac agggcaagta tatagtatcc ggacctgctt atttggttgt tgaggatata
     4321 ccatttccat tggctgttcc atttggttat ttcccggtta accaaaaata ttcgtcaggt
     4381 attatattcc caacgtttgg agaagaacaa acacgtggtt tttatgcaaa agggatggga
     4441 tattatttcg ctataaatga ctacattgat ttagccttaa ctgcagatgt gtatactaaa
     4501 ggttcctggt ctttggcatt agcttcaagt tataaatgga ggtacaagtt tggaggatct
     4561 cttaatttga gttttattcg caatacttat ggtgatccga atacacctga ttatagatca
     4621 tctaatgatt ttagagttat gtggtcacac acacaagatc agaagatgag tccgtacact
     4681 tcgttttcgg caagtgtaaa cttttcaaca tcaagttacg aacagaacaa tgtagatagt
     4741 tactacaatc cggctttatt gtctcaaaat acaaaaagtt caacaataac cttaacacag
     4801 acattcccgg agagcccatt tacattgtct ttaagtatgt atgttaatca acgtacatca
     4861 gattcaacga taaacttgaa tttgccgtca attacattaa acatgagtcg tgtttgtcca
     4921 tttaaacgaa aatcgtctgt tggtaaagaa aaatggtatg agaagattgc tattacatat
     4981 aatttggctc tttcaaacag tataactgca aaagaaagca aatttatgca gactaatttc
     5041 ttgcgagatt ggaaaaatgg tattaaaaac gaaataccta tatcagcatc atttactctg
     5101 tttaaatatt taaatttgaa tgtaggttta aataattcat tgagatggta ttttagtaaa
     5161 gtcaatcaac attgggaggg agactattcc ggtgctgcgg tatcagatac tacttatggc
     5221 ttttacaatt tatataaatg cagtgcatca gttggtttac aaacacaatt attcggattt
     5281 tacaaaataa aaagtaaacc cggtaaaaat gcgcctatat ttaggcacaa aatagttcca
     5341 tctgtaagtt tttccattgc acctgatttt ggtgcaccgg gttgggatta ttggggaacc
     5401 tacgatcgtc ctgcatctga tggttcaatt aaaaccgttt actatgataa gtttgcaggg
     5461 ggaatatatg gaggttcacc ttctagaggg gcatccggta gcgtgtcatt cac
//
LOCUS       DAOPID010000091         5504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_19567_length_5504_cov_4.288126, whole genome shotgun sequence.
ACCESSION   DAOPID010000091 DAOPID010000000
VERSION     DAOPID010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5504
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19567_length_5504_cov_4.288126"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PK641_04200"
     CDS             <1..219
                     /locus_tag="PK641_04200"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPG55584.1"
                     /translation="IEVKTRSSTRFILPQDAVTHAKIRHIVNAAQGYIHQYGIKKDVR
                     FDIMALIPGEYGGYSIDHIKDAFMSPVW"
     gene            387..1103
                     /gene="dapB"
                     /locus_tag="PK641_04205"
     CDS             387..1103
                     /gene="dapB"
                     /locus_tag="PK641_04205"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HPG55585.1"
                     /translation="MKIALIGYGKMGKTIEQIAISRGHEIVAHIDIDNQQDFDSKEFK
                     SADIAIEFTAPSVAVENYRRAFNAGVSVVSGTTGWTARMEEVKSWCKEKDLAFFWTSN
                     FSLGVNIFFAMNKYLAKIMNEFTEYEVDITETHHIHKLDAPSGTAITLADGIIENLDR
                     KSSWELDKASKDDKIGIKAIREGEVPGIHTIRYESDVDSITITHDAKSRAGLALGAVV
                     AAEFTYRKKGCLGMSDMLNF"
     gene            1115..2518
                     /gene="lepB"
                     /locus_tag="PK641_04210"
     CDS             1115..2518
                     /gene="lepB"
                     /locus_tag="PK641_04210"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPG55586.1"
                     /translation="MQKFSNIQTKQWIKAAISCITYILFIIWVGNYWWLLLLPFIADI
                     FITKIIPWSFWKKIKNKTLYSICSWLDAIIFALIAVYFINTYLFQNYRIPSSSLEKSL
                     MVGDFLLVSKASYGPRVPMTPLSFPLVQHTFPGTNLKSYIEKPHWEYKRLAGLDTIKA
                     LDIVVFNFPTGDTVATLQSNPDYYTLCEMFGRDNVLKHTETFGKIVYRPVDRRENYVK
                     RCVGLPGDSLQIIDNHIFINGKAQYEPKGLQFNYFVATDGRKISEEQFDKLGISVDDR
                     NVINQWNNGDAILEYLGYTRNSNGAFNPVYYLPLTKAALSATKKLPNVIDVKIEPNLF
                     SHQVFPKTEKDWTRDNYGPIYIPRKGATISLNMDNLPIYQRAIVNYENNELQVKDSVI
                     YINGEKSNTYTFKMNYFWMMGDNRHNSEDSRFWGFVPEDHIVGKPIFIWLSLDKDKSF
                     INSIRWKRIFSLVENIR"
     gene            2515..3129
                     /locus_tag="PK641_04215"
     CDS             2515..3129
                     /locus_tag="PK641_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HPG55587.1"
                     /translation="MSTVVLSSTYAGNIHYFSKIAHFDLVETEVYDNYLRQTYRNRCQ
                     IATADGIMDLTIPVEKPDGKCIVRDIKIADEAWQKLHWGAIMSAYNSSPYFEYYEDDL
                     RPFYTQKYKYLADFNQELEECICNLIGCKTDFCRTQKYEKEYSKDFRNLVLIKPIEDK
                     TFKQEPYYQIFKQKYDFIENLSIFDLLLNMGNESILILKKSYVE"
     gene            3119..5326
                     /locus_tag="PK641_04220"
     CDS             3119..5326
                     /locus_tag="PK641_04220"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPG55588.1"
                     /translation="MSNNLVKYSILLLLCLLHVNVSAQISSGGSPLPYKHPQPSEKAR
                     KVVATESVTNFYYKLPVDTNKTLPENNLRVLYTGTVCNVDLSPTNSGTTFTTEDGTNI
                     WRVGMYSPNAASISLIFSEFNVPDGAKLFLYNPSQTIVYGAFTSANNNSHNTLPIRPL
                     DNDTIVIEYQEPMDAAFKGSLKIGSAVHNFESSTSATRKVRQFYYAERCSPHTSVWEG
                     AEPIKRSVCILYATGTSSAWWGTANLINNADKHPYIYTAAHNVDETNVEHHVIWYFNY
                     EVPAKDTLVQGSEEFTMASSEVIARDFTLDFALLELNQMPPKDYRPYLAGWNLTASPT
                     PPLTCIQHPQGDTKRISQTTNAPVISTFDGDILNPVQNSFWRINRWQKGTTEPGSSGS
                     ALLDNNGYIIGALTGGNSTCDSPVLDYFSRISYAWDYYQDASKQLKIWLDPYNTGITQ
                     MDGADPYENSHPCARTNNYTDTDEMSIIYIGNGISGLLAGHNSLEHTAFAEKFSFDED
                     KLLYGAYVVPCIGKYNSSAPVYLNIYNGTDVPEDIIGSAQIHPQDLTFVRGELGTKDK
                     VTLTKKENYIKFDTPIKVGKTFFVGCQIQYAQIDSFGIYAVENRTINNSAFYQDDNGW
                     HSYTEHSYMPTNTSLWIEPVVCFDDISSVQNVNDNASNNFLVSPNPTSDKIQWESTNN
                     EIGDEVYFELFDIQGRLCNFGKSTLVQMPPAKGIYILRLIDGINTEVHKIIRK"
BASE COUNT         1954 a          924 c          973 g         1653 t
ORIGIN      
        1 atagaggtta aaacacggag tagtaccaga tttatattac ctcaagatgc tgttactcat
       61 gccaaaattc gtcatattgt aaatgcagcg cagggttata ttcaccaata cggcatcaaa
      121 aaagacgtcc gttttgatat tatggcttta atacccggcg agtatggagg atatagtata
      181 gaccatatta aagatgcatt tatgagtcct gtttggtgat tattggttat taatataaat
      241 actgatgcaa aaatttgtgt tccttaatgt atatcctatt tcgcacttct tatttgaatt
      301 ttcacaagtc acattcaact aaataaattt ggttacttta ctgctttgct gtatttttgt
      361 agtttataca ttaatctctg atttagatga aaatagcatt gataggatac gggaaaatgg
      421 gtaaaaccat cgaacaaata gcaataagtc gtggacacga gattgttgca cacattgaca
      481 tagataacca acaagatttt gattctaagg aatttaaaag tgcagacatc gctattgaat
      541 ttactgcacc atctgtagcc gtagaaaatt acagaagggc atttaatgcc ggagtatctg
      601 tcgtatcagg aacaacagga tggacagcaa gaatggaaga agtaaaatct tggtgcaagg
      661 aaaaagattt agctttcttt tggacttcaa attttagttt gggcgtaaat atcttctttg
      721 caatgaacaa gtaccttgca aaaataatga acgaattcac tgaatatgaa gtagatataa
      781 cagaaacaca tcatatacac aaattggatg ctcccagtgg cactgctatt acattggctg
      841 atggtattat cgaaaatcta gatcgcaaat catcatggga acttgacaaa gcaagcaaag
      901 acgacaaaat aggcatcaaa gcaatacgcg aaggagaagt tccgggaatt catacaatta
      961 gatatgagtc tgatgtagat tctataacca ttactcatga cgcaaaaagt cgcgcaggct
     1021 tggcattggg tgcagttgtt gctgcggaat ttacataccg taaaaaaggt tgcctgggta
     1081 tgtcagatat gcttaatttt taaatactaa aacgatgcaa aaattttcaa atattcaaac
     1141 caagcaatgg attaaagctg ccatttcttg catcacctat atattattca ttatttgggt
     1201 tggcaattat tggtggttgc tgcttttacc ttttattgca gatatattta tcacaaaaat
     1261 aattccttgg agtttttgga agaaaataaa gaacaaaaca ctttatagta tttgcagctg
     1321 gttggatgcc ataatttttg cattgatagc tgtatatttt ataaacacat acttatttca
     1381 gaactataga attccatcct catcactaga aaaatctttg atggttggag attttttgtt
     1441 agttagtaag gctagttacg gaccacgtgt acctatgact cctctttcat ttcctttagt
     1501 tcaacacaca ttccccggga ctaatttaaa atcatatatt gaaaaacccc attgggaata
     1561 taaaagactt gccggacttg atacaataaa agcattagat atcgttgttt tcaacttccc
     1621 tacaggagat accgttgcaa ctttacaatc taatccggat tattacactc tatgcgaaat
     1681 gtttggtaga gacaacgtac taaaacatac cgaaaccttt gggaaaatag tttatcgccc
     1741 tgtagatagg cgcgagaatt atgtaaaacg ttgcgtagga ttaccgggcg atagccttca
     1801 aatcattgac aaccatatat ttataaatgg caaagcacaa tatgaaccca aaggtctaca
     1861 atttaattat tttgtagcta ctgatggaag aaagatatct gaagagcaat ttgataaact
     1921 tggcattagc gtggatgacc gaaatgttat aaatcaatgg aacaatggtg atgccatttt
     1981 agagtatctt ggatatacta gaaattcgaa tggagcattt aatcctgttt attatcttcc
     2041 tcttacaaaa gctgcattaa gtgcaacgaa aaaattacct aatgtaattg acgtcaaaat
     2101 tgagcctaat ttattttcgc atcaagtatt tcctaaaacg gaaaaggatt ggacaagaga
     2161 caattatggc cctatatata tacctagaaa aggagcaaca attagtctga atatggacaa
     2221 tttacctata tatcaacgtg ctatagtcaa ctatgaaaac aatgaacttc aggttaaaga
     2281 cagcgtgata tatatcaatg gtgaaaaatc taatacttac acattcaaga tgaactattt
     2341 ttggatgatg ggagacaaca gacacaactc tgaagattct cgcttttggg gatttgttcc
     2401 tgaagaccac attgttggta agcctatttt tatttggctt tcattagaca aagacaaaag
     2461 ttttataaac tcaatacgtt ggaaacgaat attttcgtta gttgaaaata tacgatgagt
     2521 accgttgttt tatcatctac atacgccgga aacatacact atttcagtaa aatagcacat
     2581 tttgatttag tggagacaga agtttacgat aattatttga gacaaacata tcgtaataga
     2641 tgtcaaatag caactgcaga tggtataatg gatttaacta taccggttga aaaacctgat
     2701 ggtaaatgca tcgttcgtga tattaaaatt gcagatgaag cttggcaaaa attgcactgg
     2761 ggtgctatta tgagcgcata taattcaagt ccgtatttcg agtattacga agacgaccta
     2821 cgcccatttt acacacaaaa gtacaagtat cttgcagact tcaaccaaga attggaagaa
     2881 tgtatttgca atctcattgg atgtaaaaca gatttttgca gaactcagaa atacgaaaaa
     2941 gaatactcta aagactttcg aaacttagtt ttaataaaac caatagaaga taaaacattt
     3001 aagcaggaac cttattatca aatatttaaa caaaaatatg attttatcga aaacttgagt
     3061 atctttgact tattactaaa tatgggaaac gaaagtattt tgatattaaa aaaatcgtat
     3121 gtcgaataat ttagtaaaat actcaatatt gttgttgtta tgcttattac acgtaaacgt
     3181 ttcagcacaa atatcttcag gaggatctcc actcccttat aaacatccac aaccttcaga
     3241 gaaagcaaga aaagttgtag caacagaatc agtaacaaac ttttactaca aactacctgt
     3301 tgataccaat aagaccttgc ctgagaataa cctgagagta ttatataccg gtacagtatg
     3361 taatgtagat ttatcaccta ccaatagcgg tacaacattc acaacagaag atggtacaaa
     3421 tatttggaga gttggtatgt attctcctaa tgcagcatct ataagcttaa tatttagcga
     3481 atttaatgtt ccggatggag caaaactatt cttgtataat ccatcacaaa ctatagtata
     3541 tggtgctttc acatctgcaa acaacaattc acacaacact cttccaataa gacctttaga
     3601 caatgatact attgttatcg agtatcaaga accaatggat gcagcattca aaggaagttt
     3661 aaagataggc agcgcagtac ataatttcga atcaagcaca agcgcaacac gtaaagtcag
     3721 acaattctat tatgcagaaa gatgcagtcc acacacatct gtatgggaag gtgctgaacc
     3781 aataaaaaga tctgtttgta ttttatatgc aacaggtaca tcaagtgctt ggtggggtac
     3841 agcaaattta atcaataacg ctgataagca tccatacatt tatacggctg cacataatgt
     3901 tgacgaaacg aacgttgagc atcatgttat ttggtatttt aattacgaag tacctgcaaa
     3961 agacacctta gttcaaggtt ctgaagaatt tacaatggca agttctgaag taattgcgag
     4021 agattttact ttagattttg cgctactcga attaaatcaa atgccgccta aagactatcg
     4081 tccatatctt gcaggttgga atttgacagc atcacctaca ccaccactta cttgtattca
     4141 acatccgcaa ggagacacaa aacgaattag tcagactact aatgcacctg ttatatcaac
     4201 cttcgatggc gatatactta atccggtaca aaattcattt tggagaatca acagatggca
     4261 aaaaggaact acagagcccg gatcttccgg ttctgcccta cttgataata atggatatat
     4321 aataggagca ttaacaggag gtaattctac ctgcgatagt cctgtactag attatttttc
     4381 acgaatctcc tacgcatggg attattatca agatgcaagc aagcaactaa aaatatggtt
     4441 agacccatat aacacaggaa tcacacaaat ggatggtgca gacccatacg aaaattcaca
     4501 tccatgtgct cgtacgaata actatacgga tacagacgaa atgagcatta tatatatcgg
     4561 taatggtata agtggtttac ttgcaggaca taattcattg gagcatactg catttgcaga
     4621 aaagttcagc ttcgacgaag ataaattatt atatggagca tacgtagttc cttgcatcgg
     4681 taaatataat agttcagcac ctgtttattt gaatatatat aatggaactg atgttcctga
     4741 agatattatt ggaagtgcac agatacatcc acaagactta acttttgtta gaggtgaatt
     4801 gggaacaaaa gacaaagtaa ctttgacaaa aaaagaaaat tatatcaaat ttgatacacc
     4861 aattaaggtt ggaaaaacat tttttgtagg atgccaaatt cagtatgcac aaattgattc
     4921 cttcggaata tatgctgttg aaaatagaac aatcaataat tccgcatttt atcaagatga
     4981 taacggatgg cactcataca ctgaacattc atatatgcca acaaacactt cattgtggat
     5041 tgagcctgtt gtttgctttg acgacataag ttctgtacaa aatgtaaatg ataatgcttc
     5101 aaataatttt ttggtttctc cgaatcccac atcagataaa atacaatggg aatcaacaaa
     5161 caatgaaatc ggcgatgagg tatattttga attatttgac atacaaggca gattatgtaa
     5221 tttcggaaaa tccacattgg tacaaatgcc acccgctaaa ggaatctaca ttcttcgact
     5281 gattgatggt attaatacag aagttcataa aatcataaga aaatagttct ttaatgattt
     5341 acaaaactaa aggcatcgtt ctagaatctt ttaaatatag agacaaccaa tctattgttc
     5401 atgtattaac agaagaattt ggtaaggtct cttatttagt gtatgcacct caaagcaaac
     5461 gcgcaagcat acgtcagtct aatatgcaag ttttaagcat actt
//
LOCUS       DAOPID010000092         5502 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_19579_length_5502_cov_4.416192, whole genome shotgun sequence.
ACCESSION   DAOPID010000092 DAOPID010000000
VERSION     DAOPID010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 5502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5502
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19579_length_5502_cov_4.416192"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(547..1878)
                     /locus_tag="PK641_04225"
     CDS             complement(547..1878)
                     /locus_tag="PK641_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009230730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPG55589.1"
                     /translation="MERIFKRKLYERLLEWKKVKNGKTAIMIEGARRVGKSTLVELFA
                     QNEYESYILIDFNEASDEVKSLFSDLMNKDYIFLQLQALYNVVLKERKSVIIFDEVQN
                     CPLARQAIKYLVKDGRYDYIETGSLISIKKNINNITIPSEEERVTLYPMDYEEFRWAL
                     GDEATIPLLRTFYDKKLPLNKAHRDKMRDFRLYMLIGGMPQAVNAYIETNNFSMVDQA
                     KRGIIQLYLDDFQKLDSAGRLEKLFKEIPSQLSQTSNRFRPYAILGNVDDNKLLELMK
                     NLEDSKTTLFSFHSNDPNVGMSLTKNLSKFKIFCADTGLFVTLAFWDKDITENVIYQK
                     LLNDKLNTNLGYIYENIIAQMLVSAGNRLFYYTWPKDATHNYEIDFLLSRAAKLHPIE
                     VKSSGYNSHASLDAFCEKFSHIVDKRYLIYTKDLNKDEQTILLPFYMAMFL"
     gene            complement(2237..4432)
                     /locus_tag="PK641_04230"
     CDS             complement(2237..4432)
                     /locus_tag="PK641_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HPG55590.1"
                     /translation="MNSLRFEVVSEAFKKKPLAVSAPSVSTSDYFGKCVFDRKQMSKY
                     LSKETMKVICDVIDKGEVLDREIANHVAAGMKMWAMEMGATHYTHWFQPLTEGTAEKH
                     DSFIEYVGAPGDGIIEEFSGKLLAQQEPDASSFPNGGIRNTFEARGYTAWDPSSPAFI
                     VDDTLCIPTVFVSYTGEALDYKTPLLKALNAVDKAATDVCNYFDNSVKKVYSYLGWEQ
                     EYFLVDEGLYAARPDLLLTGRTLMGHESAKNQQLEDHYFGAIPARVQAFMKELEFEAY
                     KYEIPCKTRHNEVAPNQFELAPIYGETNLANDQNLLLMSLMHRIARKHGFRVLLHEKP
                     FAGINGSGKHNNWSLGTDTGVGLLTPGKTPEENLQFVTFLLNILMAVYKNNALLKASI
                     MTAQNAHRLGANEAPPAIISAFLGTQISAVLDKLEMSTSEEAIVMDSKKRMKLGVTHI
                     PEVLVDNTDRNRTSPFAFTGNRFEFRAVGSSANCASAMIALNAAVAGQLKEFKTAVDA
                     KIASGITKEKAIFDVLKQYIKESKSIRFDGNGYSEAWKQEAKKRNLDCETSVPIIYDA
                     YTTDASVKLFAETGVFNKIELEARNEVKWETYTKKIQIEARVLGDMAINHIIPVATRY
                     QSLLLDNVYKMMQVYDKEKAARLSAHDNSIIEELSENISIIKQKVEEMVAARKVANKI
                     SSEREKAIAYHDTVLPFFDEIRVYTDKLEMIVDDEMWTLPKYREMLFIR"
     gene            complement(4555..5385)
                     /locus_tag="PK641_04235"
     CDS             complement(4555..5385)
                     /locus_tag="PK641_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55591.1"
                     /translation="MKAAAPSVETAASPIVPLDTVWVLLAAMLVFFMQPGFALVEAGF
                     TRSKNTANIIMKNFVDFMLGTVLFWFVGFGIMFGDGGFMGVPNLFSLDFYPDSNVPME
                     AFLVFQTVFCATSATIVSGAMAERTKFYMYIIYSILISAIVYPVSGHWTWGGGWLSQL
                     SLLGYDGGFHDFAGSTVVHSVGGWLALVGASSVHGVCGFIGTLLVGLFSTTDGLFYGG
                     GIGLLAVQAVGALTVGAWALLCGFVIFKGLDKIKSLRVTPRIEEEGLDIYEHGETAYN
                     "
BASE COUNT         1715 a         1152 c          910 g         1725 t
ORIGIN      
        1 gaattgcatt cgcccttggg attcgcattg tgattatgtt tcgttgggcg attttgtagg
       61 ggcatgccat ggcatgccct tcattttcga tatgtgatta atataaattc gattgtgaaa
      121 tgatccgcaa cgtacccgta ggggcgaatt gcattcgccc ttgggattca caatgcaata
      181 aatacaaatt caatgttgaa atgttgaaat gttgaaatat tgaaatattg aaatgtataa
      241 ttaatataca ccttgttcga taaatcgtca cgtatttaat gcgaagggca tgccatggca
      301 tgcccctacg atcgcattgt gttgcggtta ttcgtgtcga aatatttgtt catgtcgaat
      361 gtgaagggcg aatgcaattc gcccctacag cgcatttatg aaacggtatc gcatttcgaa
      421 acattacacg attacacatt acacattaca cgctacacat tacacattcc acgcccctac
      481 gatctcaaaa acacacgcat ccgcaccacg caatctatac gtttattgtt tatcgttaga
      541 attttcctaa agaaacatag ccatataaaa aggaagaagt attgtttgct cgtctttatt
      601 taaatcttta gtatatatta gataacgctt atcaacgata tgcgagaact tctcacagaa
      661 ggcatcaagt gacgcatgac tattatagcc cgacgactta acttcaatag ggtgcaattt
      721 ggctgcacga gaaagtaaga agtctatctc atagttgtgt gttgcatctt taggccaggt
      781 gtaatagaat agtctgttgc cggcagaaac caacatttga gcaatgatgt tctcgtatat
      841 atagccaagg tttgtattta gcttatcatt cagcaatttt tgataaatta cattctcagt
      901 aatatcttta tcccaaaaag caagggttac gaacaaacct gtatcggcac aaaatatctt
      961 aaacttggat aaattctttg ttaacgacat tccaacattt ggatcgttag aatggaaaga
     1021 gaaaagagtt gtcttgctat cttcaagatt tttcatcaat tctaacagct tattatcgtc
     1081 aacatttcct agtattgcgt acggtctaaa acggttactt gtttgactta gctgagaggg
     1141 tatctcctta aatagttttt ccaagcgacc tgctgaatca agtttttgga aatcatctaa
     1201 ataaagttgt ataataccac gcttcgcctg atcaaccata ctaaaattat tagtttcaat
     1261 ataggcatta actgcttgag gcatgccacc aataagcata taaagtctaa aatcacgcat
     1321 tttgtctcga tgagctttgt ttaatggcaa ttttttgtca taaaatgtac gaagcaaagg
     1381 aattgtagct tcatcgccca atgcccaacg aaactcttcg taatccattg gatagagagt
     1441 aacgcgctct tcttcacttg gtatagttat attatttata tttttcttaa tacttataag
     1501 cgacccggtt tctatatagt cataacgacc atccttaact aaatacttaa tagcctgtcg
     1561 agccaaagga caattctgaa cttcatcaaa gataattaca gacttacgtt ctttaagtac
     1621 aacattatat agtgcttgaa gctgaagaaa gatataatcc ttattcatca aatcactgaa
     1681 caacgatttc acttcgtctg aagcctcatt gaagtcaatt aaaatatatg actcatattc
     1741 gttctgtgca aacaactcaa ccaaggttga tttgcctaca cgtctggcac cttctatcat
     1801 tatggcagtc tttccattct tcactttctt ccattctaga agacgttcat atagcttgcg
     1861 cttaaatatt cgttccatat tttgagtata tatttgatgc aaatataatg tagtttttcc
     1921 agttactaaa atgttttaat atcaaatatg ctaaaatacg gattttttaa tacacaaaat
     1981 atgctaaaat acggatttat atatattttt cgaaaacatg tgtagggtaa agggtgaggg
     2041 gtgaggggtg tgtaatgtgt aggggcgaat tgcattcgcc catgggattc gtggtgtgat
     2101 tatgtttttt tggtcgattt tgtagaggca cgccatggca tgcctctaca aaaaacaagc
     2161 atcaattaca gagccaaatc tctgcaatca atgcctgtct cgaaaatgat tgaggtttta
     2221 taactacccc aaagtactat ctaataaaca acatttcgcg atatttagga agagtccaca
     2281 tttcatcatc aacaatcatt tcgagtttat ctgtgtaaac acgaatctcg tcaaagaaag
     2341 gtaaaacagt atcatgataa gcaatcgcct tttcacgctc gctagagatt ttatttgcaa
     2401 ccttacgagc cgcaaccatc tcctcaacct tttgcttaat aatagatata ttctctgaca
     2461 actcttcaat aatagaatta tcatgagcac ttaaacgagc agccttctct ttatcataca
     2521 cctgcatcat cttatataca ttatctaaca ataaagactg ataacgagtt gcaaccggaa
     2581 tgatgtgatt tattgccata tcacccaata ctcttgcctc aatctgaatc tttttagtat
     2641 aagtttccca cttaacctca ttacgagctt ccaactctat tttgttaaat acacctgttt
     2701 ctgcaaatag cttaacactt gcatctgtag tataagcatc gtagattata gggacactgg
     2761 tttcgcaatc taaattacgt tttttagcct cttgcttcca cgcttcagaa taaccgtttc
     2821 cgtcaaatct aattgacttc gattctttga tatattgttt taacacatcg aaaattgctt
     2881 tctccttagt tatacctgaa gctatttttg catcaacagc tgttttaaac tcttttaatt
     2941 gacctgctac agccgcgttt aatgctatca ttgctgatgc acagtttgca gaagaaccaa
     3001 cagcgcggaa ttcaaaacga tttccggtaa atgcaaatgg tgatgttcta ttacgatcgg
     3061 tattatcaac taatacttcg ggtatatgag taactccaag tttcattctc ttcttgctat
     3121 ccattacaat agcttcttcg ctggtactca tttcaagctt gtccaaaact gccgaaattt
     3181 gagttccaag gaatgcagat ataattgcag gaggagcctc atttgctcct aaacggtgag
     3241 cattctgtgc agtcataata gatgctttta aaagcgcatt gtttttataa actgccatca
     3301 atatattaag aagaaaagtt acaaactgca agttctcttc aggagttttt cccggagtaa
     3361 gtaaaccaac accggtgtca gtaccaagag accagttgtt atgttttcct gaaccattaa
     3421 tgcctgcaaa tggtttttcg tgaagcaata cacgaaatcc gtgtttacgt gctatacgat
     3481 gcattaacga cataagaagt aagttctggt cgtttgctaa gttagtttca ccataaatag
     3541 gagctaactc aaactggttt ggtgctacct cattatgacg tgttttacaa ggaatttcat
     3601 atttatatgc ctcaaactcc aactctttca tgaaagcctg aacacgtgca ggaatagctc
     3661 caaagtaatg atcttcaagc tgttgattct tagcactttc gtgtcccatt aaggtgcgtc
     3721 ctgtaagtaa taaatcggga cgtgcagcat ataaaccttc atcaaccaag aaatattctt
     3781 gttcccaacc caagtatgaa tacactttct ttacagagtt gtcaaaataa ttgcaaacat
     3841 ctgttgctgc tttatctact gcattaagtg cctttaacaa aggtgttttg tagtctaatg
     3901 cctcacctgt atatgaaacg aatactgtag gaatacacaa tgtatcatca actataaatg
     3961 caggtgatga aggatcccat gctgtgtaac cgcgtgcttc aaaagtatta cggataccac
     4021 cattggggaa acttgatgca tcaggctcct gttgagccaa caatttgcct gaaaattctt
     4081 cgatgatccc atcgcccggt gcacctacgt attctataaa tgaatcgtgc ttttcggctg
     4141 taccttcggt taatggttga aaccagtgag tatagtgtgt cgctcccatt tccattgccc
     4201 acattttcat acctgcagca acatggttag caatttcgcg atctaatacc tcacccttat
     4261 caataacatc acaaatgact ttcattgttt ccttcgaaag atactttgac atctgtttac
     4321 ggtcaaatac gcattttcca aaataatcag aagtacttac gctcggagca cttacagcta
     4381 agggtttctt tttaaaagcc tcgcttacca cttcaaatct taatgaattc ataatctgtt
     4441 tgctttttat tagttaaatc tttatcattt cttttcaata taaaaaataa aaccaccgat
     4501 tcgttaaata actatacaaa atatacttac cgacgaatcg gggtttatta attatcagtt
     4561 ataggctgtt tcaccatgtt cataaatatc aagaccttct tcttcaatac gtggcgttac
     4621 tcgtaaactt tttatcttat caagaccttt gaaaattacg aagccgcaca ataatgccca
     4681 tgctcccact gttaaagcac ctacagcttg cactgccaat agcccgattc caccaccgta
     4741 aaacaaacca tcggttgtag aaaacaaacc taccaataat gttcctataa aaccgcatac
     4801 tccatgtact gaagatgcac ctaccaacgc caaccatcct ccgacagaat gcacaaccgt
     4861 tgaacctgca aaatcgtgaa atccgccatc atatcctaat aatgataatt gtgacagcca
     4921 acctccgccc catgtccaat ggccagaaac aggatatact atggcagaaa tcagtatcga
     4981 atatattatg tacatataaa acttggtacg ttctgccatt gctccggaca caatagttgc
     5041 agatgttgcg caaaatactg tttgaaatac aaggaaagcc tccattggta cgttagaatc
     5101 gggatagaaa tcaagtgaaa acaaatttgg aactcccata aaaccgccat caccaaacat
     5161 tattccgaaa cccacaaacc aaaacaggac tgtacccaac ataaagtcaa caaaattttt
     5221 cattataatg ttggctgtat ttttactacg ggtaaaacct gcttcaacca atgcaaatcc
     5281 cggctgcata aaaaacacta acatcgctgc caataaaacc caaaccgtat ctaagggcac
     5341 gataggactt gctgctgttt ctactgaggg cgcagccgct ttcaaaactt ctacagtttc
     5401 actaacttct gtcgcttcta ttgcgacaat atctgctgca caaatttcgc ctagagacaa
     5461 aatctctatt atgggcaaaa ttagcaataa tttaatatat cg
//
LOCUS       DAOPID010000093         5253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_20919_length_5253_cov_4.218546, whole genome shotgun sequence.
ACCESSION   DAOPID010000093 DAOPID010000000
VERSION     DAOPID010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5253
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20919_length_5253_cov_4.218546"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="PK641_04240"
     CDS             complement(<1..470)
                     /locus_tag="PK641_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPG55592.1"
                     /translation="MKKLVILFALLFTTAFAVFAQEVPQLPVDPSVRTGKLDNGLTYY
                     IRKNDYIPQRADFYIAQNVGSILEQKDQRGLAHFLEHMAFNGTKHFPGKAMINYLETI
                     GVQFGGNLNAYTSIDETVYLMRDVPVIRTGIIDSALTILRDWSDGISLETSEIE"
     gene            complement(483..665)
                     /locus_tag="PK641_04245"
     CDS             complement(483..665)
                     /locus_tag="PK641_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55593.1"
                     /translation="MNKTMMIILLIISIIGFAGSIFVSVTEFSASNVMLSVLLLAICV
                     YFAWSIKKQSNSKQSN"
     gene            complement(767..2581)
                     /locus_tag="PK641_04250"
     CDS             complement(767..2581)
                     /locus_tag="PK641_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HPG55594.1"
                     /translation="MAEKIKNIELLSPAKTAGIGIEAIRHGADAVYIGAPKFGARSAA
                     SNTIEELQTLIDYAHLFHAKVYITFNTILKDEELAEAEQLIKRLYEIGVDALIVQDMA
                     IFEMDLPPIAIHASTQTDNRTLEKVKFFESIGCSQVVLARESSLEQIKNISSNTNVAL
                     EAFVHGALCVSYSGQCYASQALCGRSANRGNCAQICRLPFDLQDSEGHSFGKYHWLSL
                     KDLDLSASLEEMIEAGISSFKIEGRLKDADYVKNITAHYRQKLDEILKKHSSWHRTSD
                     GYSNYTFVPNVCKTFHRDATDYFYHGRHNGIASLYTPKSTGEFIGTVIYSDSKSMSLD
                     TKSVLHNGDGLCYLDDKNQFQGFRVNRVEGKMLFPAESLVLKKGTQVYRNADIEYERI
                     LSKPSAIRKIPIKIALRDNILSASDGVSNATIIISQEKIIAQKPQDENIRFQLSKLGD
                     TIYESSDIEINSNWFFPSSILSDARRRMVIELNKLRIETHPKTQAFIKKNDVQFPKTE
                     LDYTGNVYNHLAEAFYKRHGVFSLQRAFEQNAQQNLTLMFCKHCIKYTLGACLNHPNP
                     DVPMLKTPLQEPLHLIYKNYYLRLQFDCKNCEMHVIAE"
     gene            complement(2610..5228)
                     /gene="alaS"
                     /locus_tag="PK641_04255"
     CDS             complement(2610..5228)
                     /gene="alaS"
                     /locus_tag="PK641_04255"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPG55595.1"
                     /translation="MMTSKEIRKSFLDFFASKGHKIVPSAPMVIKDDPTLMFTNAGMN
                     QFKNIILGNDPIKYPRIADSQKCLRVSGKHNDLEEVGHDTYHHTMFEMLGNWSFGDYF
                     KSEAIQWAWEYLTEVLKLDKNRLYVTVFEGAPAEGLSRDDEAANIWAKYIDPSRIING
                     NKHDNFWEMGDTGPCGPCSEIHIDLREDTERAKIDGKTLVNGDHPQVIEIWNNVFMQY
                     NRKSDGSLEALPKKVIDTGMGFERLCVAVQGKKSNYDTDVFIPLLKAVGESCSCEYGK
                     DNKIDIAMRVVADHIRTISFAITDGQLPSNAKAGYVIRRILRRAVRYGYTFLNQRSAF
                     MYKLVPALIDNMGEAYPELKLQQTLIEKVIKEEEDAFLRTLETGIRLLDKVMSDTKKN
                     GRDEISGVDAFVLYDTYGFPIDLTELILKENGLKLNHNDFDAEMQKQKDRARNAASIE
                     TGDWVIIREGETAFVGYDQTEADAQILRYRKMVQKGKEFYQVVLSVTPFYAEMGGQVG
                     DSGVLETESEKIQVIDTKKENNLPIHILTTLPKQIEATFKAIIDIDNRMATEANHTAT
                     HLLHEVLRDLLGQHVEQKGSYVSPESLRFDFSHFQKLTPEEIRKVEHAVNKKIRMNEP
                     LHERRNVPIAEAKSLGAMALFGEKYGDSVRVIQYGSSIELCGGTHAKSTGNIGIFKIV
                     SESSIAAGVRRIEAVTAEHAEFLIDDLQNMQVSVREMFNNVPDVVQAAQRTLEDNTAL
                     RKKIDIFMQERALQIRDNMLNRAEQFGDVKLIRYVGNDNPDIFKNIVPYFKGKFTDVK
                     FVFVAGTVYEDKPTLTILLSKPMVDAGYNAGNIIREAAKFIQGGGGGQAFMATAGGKY
                     ANGIDEAIEAVVEAIK"
BASE COUNT         1615 a         1011 c          793 g         1834 t
ORIGIN      
        1 ttttcaatct cgcttgtttc caatgagatt ccgtctgacc aatctcttaa aattgtaagt
       61 gcgctatcaa taattcctgt tctaataaca ggaacatcac gcatgaggta cacagtttca
      121 tcaatacttg tgtaggcatt taaatttcct ccaaattgta ctccaatggt ctccaagtaa
      181 ttaatcattg ctttaccggg gaaatgtttg gttccgttaa atgccatgtg ctccaaaaaa
      241 tgagccaacc cgcgttggtc tttttgttcc aaaatagaac ctacattttg agctatataa
      301 aaatcagctc tttgtggaat atagtcattt ttacgtatat agtacgttaa gccgttatca
      361 agttttccag ttcgaacact tgggtctacc ggcaattgtg gaacctcttg agcaaatact
      421 gcaaatgcag ttgtaaaaag tagagcaaat aaaattacta gttttttcat tatatattat
      481 ttttaattag attgttttga attagattgt ttttttattg accaagcaaa ataaacacag
      541 attgctaaca ataacactga aagcattaca ttactggctg aaaattctgt aacagataca
      601 aatatcgatc cggcaaaacc tattatgctt ataatcagca aaataatcat cattgtttta
      661 ttcataggta ttaaatttgt ttataatatt tcgaattagt acaatagcag ttttatgcaa
      721 aacaagccgc aaaggtacaa tattaattaa acatataaaa ctattatcac tctgcaatta
      781 cgtgcatctc acaattttta cagtcgaatt ggagtcttag ataataattc ttatatatta
      841 aatgcaaggg ttcttgcaaa ggtgttttaa gcattggtac atcaggattt ggatgattaa
      901 gacaagctcc aagggtatat ttaatacaat gtttacaaaa cattaaagta aggttttgtt
      961 gtgcattttg ttcgaatgct ctttgcaatg aaaacacacc atgtcgttta taaaaagctt
     1021 ctgccaaatg attatataca tttccggtat aatctaattc tgttttagga aattgtacat
     1081 cattcttttt tataaatgct tgtgttttag gatgtgtttc aatgcgtaat ttatttagct
     1141 caattaccat acgtctacga gcatcagaca gaatcgaaga tggaaaaaac caattactat
     1201 ttatctcaat atcagatgat tcataaattg tatcacctaa tttagataat tgaaaacgaa
     1261 tattttcatc ttgaggtttt tgtgctatga ttttttcttg agaaataata attgtggcat
     1321 tacttactcc atctgatgca gataaaatat tatctcgtaa tgctattttt ataggtattt
     1381 ttcgaatagc agatggtttt gacagtattc tttcatattc tatatctgca tttctgtaga
     1441 cctgtgtacc tttcttcaaa actaatgatt ctgcaggaaa caacatctta ccttcaacac
     1501 gattcactct aaaaccttga aactgatttt tgtcgtctaa ataacataag ccatctccat
     1561 tatgtaatac agattttgta tcaagtgaca tacttttaga atcagaatat ataactgttc
     1621 ctataaattc gccggtcgat ttaggcgtat aaagagatgc tattccgttg tgcctgccat
     1681 gatagaaata atccgttgca tccctatgaa aggttttaca aacatttggt acaaaagtat
     1741 agttactata accatcagaa gttctgtgcc atgaagaatg tttctttagt atttcatcaa
     1801 gtttttgacg ataatgagct gttatattct taacgtaatc tgcatctttt aatcgtcctt
     1861 cgattttaaa tgatgagatt ccggcctcta tcatttcttc gagtgatgct gacaaatcta
     1921 aatctttcaa cgacagccaa tgatattttc caaagctatg tccttcgcta tcttgcaaat
     1981 cgaatggcaa acgacatatt tgagcacaat taccacgatt ggcacttcgt ccacataaag
     2041 cttggcttgc ataacactgc ccactatagc ttacacataa tgctccatgc acaaatgctt
     2101 caagagcaac atttgtatta gaactgatat tctttatttg ttcgagtgaa ctttcgcgag
     2161 ctaaaacaac ttgactacaa cctatacttt caaagaattt tactttttcg agggttctat
     2221 tatcagtttg cgtacttgca tgaatggcaa ttggaggtaa atccatttca aagatggcca
     2281 tatcttgtac aatcaaagca tcaaccccta tttcgtataa tctttttatt aattgttcag
     2341 cttcggctaa ttcttcatcc tttaagattg tattaaaagt gatgtaaact ttagcatgaa
     2401 acaaatgagc gtaatcaatt aatgtttgaa gttcttcaat tgtatttgat gcagcagacc
     2461 ttgctccaaa cttaggagca cctatatata cggcatcggc accgtgccta atagcctcga
     2521 tgccgattcc tgccgttttt gccggagata ataattctat attttttatt ttttctgcca
     2581 ttaagcaaat caaatactaa aaaaatctgt tatttaatgg cctcaaccac agcttcaatt
     2641 gcttcgtcaa taccattagc atatttaccc cctgctgtag ccataaatgc ttgaccaccg
     2701 ccaccgcctt gaatgaattt tgctgcttca cgaataatat ttcctgcgtt ataacctgca
     2761 tctaccatcg gcttacttaa taatatggtc aatgtcggtt tatcttcata aacagtacct
     2821 gctacaaata caaacttaac atctgtaaat ttacctttga aataaggtac aatgtttttg
     2881 aatatatcag gattatcatt acctacatat ctaataagct taacatcgcc aaattgttct
     2941 gccctattaa gcatattatc acgtatctgt agagctcttt cttgcataaa aatatcaatc
     3001 tttttacgaa gtgctgtatt gtcttccaaa gtacgttgag cagcttgtac tacatccggt
     3061 acattattaa acatttcgcg cacagatact tgcatatttt gcaaatcatc tatcaaaaac
     3121 tctgcatgtt ctgctgttac agcttcaata cgacgtacac cggctgcaat agaactttca
     3181 gaaactattt taaatatgcc tatgttacct gttgattttg cgtgtgttcc accacataat
     3241 tctatagaag aaccatattg tataacacga acgctatctc catatttttc gccaaataat
     3301 gccattgcac caagtgattt agcttctgca atcggtacat tgcgacgttc gtgcaaaggc
     3361 tcattcatac ggattttctt atttactgca tgttcaactt tacgtatttc ttcaggtgtc
     3421 agcttttgga aatgcgagaa gtcgaaacgt aatgattcgg gagaaacata agaacctttt
     3481 tgctcaacat gttgacctag taaatcgcgc aaaacctcat gtaataagtg agttgcggtg
     3541 tgatttgctt cagttgccat acgattatca atgtcgatta tagctttaaa agtagcctca
     3601 atctgttttg gcaaagtggt taatatgtga atcggcaaat tattttcttt ttttgtatct
     3661 atcacttgaa ttttttctga ttcggtttca agtacacctg aatcgccaac ttgaccaccc
     3721 atctcagcat aaaaaggcgt aacagacaaa acaacttgat aaaattcttt gcctttttga
     3781 accatctttc tataacgtaa aatttgtgca tcagcttcgg tttgatcata tcctacaaat
     3841 gcagtttcgc cctcgcgtat tattacccag tcgcccgttt caatagatgc tgcattacgt
     3901 gcacgatctt tttgtttttg catttctgca tcaaaatcgt tatgatttaa ttttaatccg
     3961 ttctccttta atataagttc ggttaaatct attggaaaac cataagtatc ataaagtaca
     4021 aatgcatcaa caccgctaat ctcatctctt ccgtttttct ttgtatcaga cattacttta
     4081 tctaacaaac gaattccggt ttccaaagta cgtaaaaatg catcttcttc ttctttgata
     4141 actttctcaa tcaaagtttg ttgtaactta agttcgggat aagcttctcc catattatca
     4201 atcaaagccg gcaccaattt atacataaat gcagaacgtt gatttaagaa tgtataacca
     4261 tacctaactg ctctacgaag gatacggcgt attacataac ctgcttttgc attggaaggt
     4321 agttgaccat ctgtaattgc aaacgatata gttctaatat ggtctgcgac aacgcgcatg
     4381 gctatatcaa tcttattatc tttaccatat tcgcaggagc aagattcgcc tacagccttt
     4441 aataaaggaa taaatacatc tgtatcgtag tttgattttt taccttgaac agcaacacac
     4501 aaacgttcaa agcccatacc tgtatcaata actttctttg gtagagcctc taaagagcca
     4561 tctgattttc ggttgtattg catgaataca ttattccaaa tttcaataac ttgaggatga
     4621 tcgccattaa caagtgtttt gccatcaatt ttagctcttt cggtatcttc acgtaaatct
     4681 atatgtattt ctgagcaagg tccacaagga ccggtatcac ccatttccca gaaattatcg
     4741 tgtttatttc cgtttattat tcgacttgga tcaatatatt ttgcccaaat atttgcagct
     4801 tcatcatctc tactcaaacc ttcggcagga gcgccttcaa aaactgttac atataggcga
     4861 ttcttatcta attttaatac ttctgtaaga tattcccacg cccactgaat tgcttctgat
     4921 ttaaaataat caccaaaact ccagttacct aacatttcga acattgtatg gtgatatgta
     4981 tcatgtccaa cttcttccaa atcattatgt ttaccgctta cgcgtaaaca tttttgtgaa
     5041 tcagctatac gtggatattt aattggatca ttacccaaaa tgatattttt aaattgattc
     5101 atacctgcat tggtaaacat caaggtagga tcatctttaa ttaccattgg tgcagaagga
     5161 actattttat gcccttttga agcaaaaaag tccaaaaagg acttacgtat ttctttagaa
     5221 gtcatcatta tactatatga tttttttgac ttg
//
LOCUS       DAOPID010000094         5231 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_21037_length_5231_cov_4.670015, whole genome shotgun sequence.
ACCESSION   DAOPID010000094 DAOPID010000000
VERSION     DAOPID010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5231
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21037_length_5231_cov_4.670015"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="PK641_04260"
     CDS             <1..807
                     /locus_tag="PK641_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPG55596.1"
                     /translation="PLPYNAIEGLEHIDKIISVDQSPIGKTPRSNPATYTNLFADMRN
                     LFVELPEAKIRGYKPGRFSFNVKGGRCEVCGGNGYKRLEMGFMADVYVPCESCNGKRY
                     NRETLEVRFKGKSIGDVLDMTINQAVEFFENIPQLMQKLKVLQDVGLGYIKLGQSSTT
                     LSGGENQRVKLATELAKHDTGKTLYILDEPTTGLHFEDIHVLLNVLDKLVERGNTLIV
                     IEHNLDVILHADYLIDMGNEGGRNGGNVVCVGTPESIASRGIGHTAKFLK"
     gene            804..1265
                     /locus_tag="PK641_04265"
     CDS             804..1265
                     /locus_tag="PK641_04265"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HPG55597.1"
                     /translation="MTEKISAYYSKAASYCSIAERCEQEIIEKMAKWGTPEDEQQAII
                     NKLIEDDFLNERRYATAFARDKFRFNQWGRIKISYALKLKGISSHNIREGLSAIDEYI
                     YRQTIKKLTVAKQKSIKDASPSVVYVKTLKYLISKGFESDIISQILKSTES"
     gene            1271..2509
                     /locus_tag="PK641_04270"
     CDS             1271..2509
                     /locus_tag="PK641_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55598.1"
                     /translation="MKRTIIISVILYLFTTCTYAVSSAIIYGNDPEYAGDSLILYTER
                     DGLTHHQIEIGNCLVAQDGSFSINAKIDSTEKCYINLGAVIVSIFIEPGSSYSINLPA
                     KKPLTKANKLNPYFKAQEILLSINNPKPDDINLQIAAFEDDMDKEWAALVFGEINKQN
                     IEEACIRMENKYNSDNDFFRIYRFSNYALIANLYEPTSPDLAIKTYFLDEPIHYNNPA
                     YWEAFYVLFEKFIEPERLYANKPLYELVVMQKVMNKELPIKQLDKVRSEHNTIIANEI
                     KTQLNFLGVGTLLNIQKLVNINGDTLQWKDFDCPLVYIVFANSSIKESKADIAFAKKI
                     QSKWKNKCAMIFIFDKEPREKTLKTAQKNELNFFTFSTQDNPEIITTFAINNIPNYIL
                     IDYEGRIIQNPAKKPTQFTP"
     gene            2506..3240
                     /gene="pssA"
                     /locus_tag="PK641_04275"
     CDS             2506..3240
                     /gene="pssA"
                     /locus_tag="PK641_04275"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HPG55599.1"
                     /translation="MNIKNHIPNTITCLNLFSGCIAVALAFQGNLLASALFIFASAIF
                     DFFDGFAARKLKAFSIIGKDLDSLADLISFGLAPATMVSSLLKEYQYPEYMFDYAFIF
                     PYIAYLIAVFSALRLAKFNNDERQSNTFIGLPTPANALFIAGFANAPSASFLWQPLTS
                     FSDMWTNQVSGLFVLLGLIAISSFLLVSPIPMFSLKQTGKLQYVFLTISVILIGTCGF
                     LGMFMSISVYILFSIFKIIATSKTIK"
     gene            3240..3461
                     /gene="yidD"
                     /locus_tag="PK641_04280"
     CDS             3240..3461
                     /gene="yidD"
                     /locus_tag="PK641_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005655675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HPG55600.1"
                     /translation="MKKVLSWILLLPVYFYRALISPLTPASCRYTPTCSEYMIQAIKK
                     HGPIKGLFLGLKRLSKCHPWGGSGYDPVP"
     gene            complement(3465..5228)
                     /locus_tag="PK641_04285"
     CDS             complement(3465..5228)
                     /locus_tag="PK641_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HPG55601.1"
                     /translation="MFLYGMKLMSEGLQKIAGEKLRSVLTAMTKNRVLGLLTGLLITA
                     VIQSSSATTVMVVSFVNAGLLTLIQSITVIMGANIGTTVTAWIISLFGFKVSISALAL
                     PIIAVSIPFIFSSHNKRKSFGEFLLGFALLFMGLELLKTSVPDLQSNPEILAFLTQYT
                     QYGFGSVFIFLLIGTILTIVVQSSSATIAITLIMCGKGWIPFELAAAMVLGENIGTTI
                     TANIAAIPANISAKRTALSHFMFNVFGVIWMLCIFFPFINMISGVVAEYGPGDPTKFS
                     EFSMGIDAQTMSLINNPKSVLTPDQLELKSQYEAYQLSTSYGLSLFHTMFNIINSFLM
                     IWFVKLIAKFVTFCIPQKSSDEEYKLTYISSGLLSTSELSILQADKEIDIYAKRSVKM
                     FGIVRALYDERDSDRFEKTYERVEKYERICDRMEIEIANYLTKVSEGKLSDKSKQDVH
                     AMLRVISEIESIGDGCHNISNILKRKRNDNIEYSDEMNIRVYAMFDLVQKSLDEMMLV
                     LHRTDNDRKVPDMKRSLEIEQEINDMRSKYKMQNATDVNQRKYPYPISVTYMDLISEC
                     EKVGDYVVNVVEQYCETREVK"
BASE COUNT         1810 a          950 c          858 g         1613 t
ORIGIN      
        1 ccgctaccat ataatgcaat agaaggatta gaacacatag acaaaataat atctgtagat
       61 caatcaccta tcggaaaaac acctcgttct aacccggcaa catacacaaa cttatttgca
      121 gatatgagaa atttgttcgt tgaattacca gaagcaaaaa tacgaggata caaacccgga
      181 cgtttttctt ttaatgttaa aggaggacgt tgtgaagtat gtggaggaaa tggttacaaa
      241 cgtcttgaaa tgggttttat ggccgatgta tacgttccat gcgaatcctg caacggtaaa
      301 agatataaca gagaaacact tgaagtaaga tttaagggga aatccattgg tgatgtactt
      361 gatatgacaa ttaaccaagc agttgagttt tttgaaaaca tacctcaact gatgcaaaaa
      421 ctcaaagtac tacaagacgt cggattagga tacatcaaat taggacaatc ttccactaca
      481 ttatcaggtg gagaaaatca gagagttaaa ttagctacag aactagcaaa acacgataca
      541 ggtaaaacac tatacattct tgacgaacca accacaggat tacatttcga agatattcat
      601 gtattactta atgttttaga caaattagta gaaagaggca atacattaat cgttattgag
      661 cataatttag atgttatact tcatgccgat tatcttatag atatgggaaa tgaaggaggt
      721 agaaatggtg gaaatgttgt ttgtgtagga acaccggaat ctattgcttc aagaggtatc
      781 ggacatacag caaaattttt aaaatgacag agaagatttc tgcatattac tcaaaagctg
      841 cctcatattg ttctatagca gaacgatgtg aacaagaaat aatcgaaaaa atggcaaaat
      901 ggggaactcc cgaagatgag caacaggcta taataaacaa attaattgaa gacgattttt
      961 taaatgaaag acgttacgca acagcatttg cacgagacaa atttcgtttc aatcaatggg
     1021 gccgcataaa gatttcatac gcacttaaat taaaaggaat aagcagccac aatattcgag
     1081 aaggcttatc agccatagat gaatatatat atcgccaaac aattaaaaaa ttgactgtcg
     1141 caaagcaaaa aagcatcaaa gatgcatcac cgtcagttgt atacgtaaag actctcaaat
     1201 accttatcag taaggggttt gagtcagata ttatttcaca aattttaaaa tcaaccgaaa
     1261 gctgaactat atgaaaagaa ctattattat atctgttata ttgtatctat ttacgacctg
     1321 tacttatgcc gtatcttctg caataatata cggaaatgac cccgaatatg ccggtgatag
     1381 tttaatttta tatactgaga gagatgggct tactcatcat cagattgaaa taggtaattg
     1441 cttagttgct caagatggtt ctttcagcat aaatgctaaa attgattcaa cggaaaaatg
     1501 ttatattaat ttgggcgcag taattgttag catctttata gagcccggat catcatattc
     1561 gataaaccta ccggctaaga aacctcttac aaaagccaac aaactaaatc catactttaa
     1621 agctcaagag atattactga gtataaacaa tccgaaaccg gatgatataa atctgcaaat
     1681 tgccgcattt gaagacgaca tggacaaaga atgggcagct cttgtatttg gcgaaataaa
     1741 taaacaaaac atcgaagaag cttgtatccg aatggaaaat aaatacaatt cggataatga
     1801 tttctttaga atatatagat tctcaaatta tgcattaatt gctaacttat acgaacccac
     1861 atctccggat cttgcaataa aaacatattt tttagatgag cctatacatt ataataatcc
     1921 tgcatattgg gaagcgtttt atgtactgtt tgaaaaattc attgagcctg agcgactata
     1981 tgctaacaag ccattatatg agttggttgt aatgcaaaag gttatgaata aagagttacc
     2041 tataaagcaa ctagacaagg taagatctga acataataca atcattgcaa acgaaattaa
     2101 aactcaacta aatttcttag gagtaggcac tttattaaat attcaaaagc ttgttaatat
     2161 aaatggggat actctgcaat ggaaagattt cgactgcccg ctagtttata tagtttttgc
     2221 caattcttct attaaagagt cgaaagccga tattgctttt gcaaaaaaga tccaaagtaa
     2281 gtggaaaaac aaatgtgcaa tgatatttat tttcgacaaa gagccacgcg aaaaaacttt
     2341 aaaaacagct cagaaaaacg agttgaactt ttttacattc agcacgcaag acaaccccga
     2401 aataataact acatttgcaa tcaacaatat ccctaattat atattaatag attacgaggg
     2461 acgtattatt caaaatccgg ctaaaaaacc aacgcaattt acaccatgaa cattaaaaac
     2521 cacataccca acactataac ttgtttgaat ctattttcag gatgcatcgc agtagctttg
     2581 gcttttcaag gcaatctact tgcatctgct ttatttattt ttgcatcagc tattttcgat
     2641 ttctttgatg gctttgcagc tcgtaaatta aaagcttttt ctataatagg caaagacctt
     2701 gattctttgg cagacttaat tagttttgga ttagcaccgg caacaatggt atcttcttta
     2761 ttaaaagaat atcagtatcc ggaatacatg tttgattatg cattcatctt tccatacata
     2821 gcatacttaa ttgctgtttt ttcggccctg agattggcta aatttaacaa tgacgaaaga
     2881 caaagtaata catttattgg cctacctacc ccggcaaatg cgctatttat tgcaggattt
     2941 gcaaatgctc cttctgcatc attcttatgg caaccactta caagcttttc cgatatgtgg
     3001 acaaatcagg tatccggttt attcgtatta ctgggattaa ttgctatctc ttcattcctt
     3061 ttggtgtcac caattccaat gttttcgctc aaacaaacag gaaaattaca atatgtcttt
     3121 ttaacaattt ctgtaatact tataggaacc tgcggtttct tggggatgtt tatgtctatc
     3181 tcggtatata tattattttc aatatttaag ataatagcta cgagcaaaac aataaagtga
     3241 tgaaaaaagt tttatcatgg attctgctac ttccggtata tttttatagg gcattaattt
     3301 cgccactaac cccggcatct tgcaggtata ctccgacttg ctctgaatac atgatacagg
     3361 caataaaaaa acatggaccg ataaagggat tatttcttgg actaaaacgc ctatcaaaat
     3421 gccacccatg gggaggtagc ggctacgacc ccgtaccgta agttttactt cacttctctg
     3481 gtttcacaat attgttcaac aacatttacc acataatcac caaccttctc gcactcagat
     3541 attagatcca tatatgtaac actaatagga tatggatact tgcgttgatt tacatctgta
     3601 gcattttgca tcttatattt gctacgcata tcgtttattt cttgctctat ttcaagtgat
     3661 cgcttcatat caggaacttt tctgtcattg tctgttctat gaagtactaa catcatttca
     3721 tcaagtgact tttgaactaa atcaaacatt gcatacactc gaatgttcat ctcatccgaa
     3781 tattcaatat tatcgtttct tttacgctta aggatgttag atatattatg gcatccatct
     3841 cctatacttt caatttctga tattacacga agcattgcat gtacatcttg cttactctta
     3901 tcacttaatt taccttcaga tacttttgtc aaataatttg cgatttctat ctccattcga
     3961 tcacaaattc tctcgtattt ttcaacccgt tcatacgttt tctcaaatct gtcagaatcc
     4021 cgctcatcat ataatgctct aacaatacca aacatcttta cgctgcgctt agcatatata
     4081 tcaatttctt tgtcggcctg taaaattgat aattctgagg tcgacaataa accggaagaa
     4141 atataggtaa gtttatattc ctcatcagat gatttttgtg gaatacagaa ggtaacaaat
     4201 ttggctatta acttgacaaa ccatatcatt aaaaatgagt taataatatt gaacatggta
     4261 tggaacaatg atagcccgta tgaagtagat aattgatatg cttcgtattg gctttttaat
     4321 tccaactgat caggtgttaa tacagattta gggttgttta ttaaagacat ggtttgggca
     4381 tcaatcccca ttgaaaactc agaaaattta gtgggatctc ccggaccata ttcagcaaca
     4441 actcctgaaa tcatattaat aaaagggaaa aatatacata gcatccaaat tacgccaaac
     4501 acattaaaca taaagtgcga aagtgcagta cgctttgccg atatattagc cggaatagct
     4561 gcaatattag ccgttatagt tgttcctata ttttctccta aaaccatcgc agccgctaac
     4621 tcaaacggaa tccacccttt cccacacata attaacgtga tcgcgatagt tgcagatgat
     4681 gattgaacta caatagtaag tatagttcca ataagtagaa aaataaacac cgaaccgaat
     4741 ccgtattgtg tgtattgagt taaaaacgct aatatttcag gattactttg taaatcaggt
     4801 actgatgtct ttaatagttc aagacccata aataacaagg cgaatcctaa caagaattcg
     4861 ccaaatgatt tacgtttatt atgtgatgaa aatataaatg gaattgaaac tgctattata
     4921 ggaagagcaa gggcagatat acttacctta aacccaaata atgagataat ccatgcggtt
     4981 acagttgttc ctatatttgc ccccatgata acagtaattg actgaatcaa tgttagcaat
     5041 ccggcattca caaaacttac taccattaca gtagttgcag atgaagactg tattacagcc
     5101 gttattagaa gacctgttaa taagcctaaa actctatttt tggtcatcgc tgttaatacc
     5161 gatcttagtt tctctccggc tattttctga agtccttcac tcatcaactt cattccatat
     5221 agaaacaagc c
//
LOCUS       DAOPID010000095         5195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_21240_length_5195_cov_4.620623, whole genome shotgun sequence.
ACCESSION   DAOPID010000095 DAOPID010000000
VERSION     DAOPID010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21240_length_5195_cov_4.620623"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PK641_04290"
     CDS             complement(<1..181)
                     /locus_tag="PK641_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55602.1"
                     /translation="MKKNILFIILFCLSSLAVFAEVGNLYFMDYLPYRVYMNPALRPI
                     CGTYVELPGISTVSLE"
     gene            complement(195..1931)
                     /locus_tag="PK641_04295"
     CDS             complement(195..1931)
                     /locus_tag="PK641_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55603.1"
                     /translation="MTKIKHLVRISLTGLLSCCLFSCVNPNYSLLEEIDTEIGLDMTL
                     AGPIGHSNLNLIKMLPDSFGSFRLVNEGDEIYLEGSNIEEMGNEIVGHLKMYPKGIFT
                     KEVSIVADGLDPYRGNISMPLTYTFDDINTNPNERLDSILYAKNQTLHIDITSDLACQ
                     TGSYVDLIFNPNELSLDPILYPENKVRADLTNGSIDIPITMDAAMLKFYGNKSFTIQV
                     EGVIISPDVIPSGFYVHLTCDYGTIKPRVTYGYLGPDRVIYETTKKMPFTYTKTLQNG
                     SFFLPFYDPKINITCESNIGIPANYTLDYVKVFDSKTGEFVYADFNGSMSTSFVLECP
                     SLDEIAGLTREELLNFDVSGIIKTTEKTFDREYGHTNRLFTINGDSMEYHYTIRPLDT
                     VVPVVSYFFDESDMDLDIDTKLLCQFEGNSTDPDKNFYINSRDTIPISIGTFDLGPVT
                     IADTTVAAVKMVHINHLPVGALAHVYFIDENKQQILTELELDITINAAPVNADGEVTE
                     ATAETKTYIKFNYEQLEIFSESCKGIVLDYRVDNDLMCNVWFKSNDWLDVKLGAYVIG
                     KIGYKPEQNNDN"
     gene            complement(1990..3381)
                     /locus_tag="PK641_04300"
     CDS             complement(1990..3381)
                     /locus_tag="PK641_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPG55604.1"
                     /translation="MLEKYVADKILAHFGFEPTRQQVDLINLLSVFITNREERSLFLL
                     KGFAGTGKTTVIGALVKAFLEMEQMIVLSAPTGRAARVLSLYAGIQAHTIHKLIYRQS
                     KPEIGGAFEIAYNKAKNTLFVVDEASMLADSGEKIFGSGNLLDDLIQFVYQGENCRLL
                     LLGDTAQLPPVTCEESPALDVRSLETFGLTITEYTLTQVVRQAKESGILQNATRLRML
                     IDNPQAVAFKEFNDVIRVDGMNFLECMENSYREVGEDETIVITRSNKRAGMYANGIRG
                     RILYREDVLSNGDLLMIVKNNYTVGLPYGLDFIANGDVAELIRTRGARTIYGCSFREV
                     SLHLVDYNMDIDALFLEDSLLSETPFDLQELNKRLYDAVMEDYAEIRNKRERYKKMRD
                     NPYLNAIQAKSAYAVTCHKAQGGQWKHVYIDMGLITADKMDKSFVRWLYTSVTRATEK
                     LYLINFPDTFFVK"
     gene            3460..4095
                     /locus_tag="PK641_04305"
     CDS             3460..4095
                     /locus_tag="PK641_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55605.1"
                     /translation="MDKVLKIVLALAAICLTWLCYQSINTPIEFNEQRDSREKKVVSR
                     LVDIRRAEVEFKNIKGDYTTNFDTLIAFVKNSKAKIVLKEGVLTDKQLEEGLTEREAV
                     KQGIIKRDTTYVSMLESLFGKDYPIDSIRYIPYSEPRQEFELAKAEIATGAAGIKVKV
                     MEAKAPFSVYLNGLDKQELVNLIELTEKLERYPGLKFGDIQQSNNNAGNWE"
     gene            4076..4795
                     /locus_tag="PK641_04310"
     CDS             4076..4795
                     /locus_tag="PK641_04310"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HPG55606.1"
                     /translation="MPETGNNPDLHSLSIRISTDGFSFCTNNEKKELYFTKKDPDFNR
                     CKAICLCEPSLQKDYGKTTIFYNGIFATLVPSTITDSTNLESILLFNFPEISSDKFDY
                     ISNAVIGYDITYIFAIPKALHNFLDDTFPACKIMHANSITLQIALKDSKKQGEKLVWA
                     DVSKSEAYIILAEEGKLKFANRFELTGDKDILYYCGCVFEQFALSQHNDKLYVSGDIT
                     CIDTLQNYFANCIYKETNICV"
     gene            4786..>5195
                     /locus_tag="PK641_04315"
     CDS             4786..>5195
                     /locus_tag="PK641_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HPG55607.1"
                     /translation="MRIISGLYKGRHFTPPANLQARPTTDFAKEGLFNLLDNRMDLSG
                     INALDLFSGTGSICYELASRGANSVTGIEQNYKHVDYIKKTASVLKINCLQILKADVF
                     KFLETCHTQYDFVFADPPYTLSNLQSIPDLIFAKS"
BASE COUNT         1799 a          960 c          775 g         1661 t
ORIGIN      
        1 cttccaaact aacagtagaa ataccaggaa gttctacgta agttccgcaa ataggacgaa
       61 gagccggatt catatacaca cggtatggca aataatccat gaaatacaga ttgccgactt
      121 cggcaaaaac agctaaagac gacaaacaaa acaatattat gaataaaata ttttttttca
      181 ttttattttc tcctttaatt atcgttgttt tgttccggtt tataaccgat ttttccaata
      241 acatatgcac caagtttaac atctaaccag tcgttcgatt taaaccatac gttacacatc
      301 aaatcattat caactctgta gtctaataca attcctttac aactttcaga aaaaatctct
      361 aattgctcat aattaaattt gatatatgtt tttgtttcag ctgtcgcttc cgtaacttct
      421 ccatctgcat ttacaggagc cgcattaata gtaatatcaa gttccaattc ggttaaaatt
      481 tgttgcttgt tttcgtcaat aaaatataca tgagccaatg ctcctacagg caagtgatta
      541 atatgtacca tttttacagc tgcaactgta gtatcggcaa ttgtaacagg tccaagatca
      601 aatgttccta ttgatatagg aattgtatct ctcgaattaa tgtagaaatt tttatccgga
      661 tctgtcgaat tcccttcaaa ttgacaaagc agttttgtat caatatctaa atccatatca
      721 ctttcatcaa agaaatatga cacgaccggt actacggtgt ctaaaggcct gattgtataa
      781 tgatattcca ttgaatcacc attaattgta aacaagcgat ttgtgtgtcc atattcacga
      841 tcaaatgttt tttctgttgt tttgattata cccgatacgt caaaattcaa caattcttct
      901 cttgtcaatc ctgcaatctc atctaatgat ggacattcaa gtacaaaact tgtactcata
      961 cttccattaa aatcagcata aacaaattct cctgttttag aatcaaaaac tttcacataa
     1021 tctaatgtat aattagcagg aatacctata ttgctctcac acgtaatatt gattttggga
     1081 tcgtaaaatg gcaagaaaaa actcccgttt tgcaaagttt tcgtgtaggt aaatggcatt
     1141 ttttttgtag tttcgtatat aacacggtca ggtcctaaat aaccataagt tactcttggc
     1201 tttattgttc catagtcaca tgttaaatga acgtaaaaac cgctaggtat tacatcggga
     1261 gagataataa ctccttctac ttgaatagta aaactcttat ttccataaaa tttaagcata
     1321 gctgcatcca tggtaatagg tatatctatt gacccatttg tcaaatctgc tctaacttta
     1381 ttctcaggat ataaaatagg atctaaagat aattcattgg gattaaatat taaatccaca
     1441 taagagcctg tctgacatgc gagatctgaa gttatgtcta tatgtagagt ttggttttta
     1501 gcatataaaa tactatccaa acgttcgtta ggatttgtat ttatatcatc aaatgtataa
     1561 gtcaaaggca tggaaatgtt tccacgatac ggatctaaac catctgcgac aatagaaact
     1621 tcttttgtaa atattccttt cggatacatt tttaaatggc caactatttc attacccatt
     1681 tcttcaatgt tcgatccttc caagtatatt tcatcaccct cgtttactaa gcgaaatgag
     1741 ccgaaagaat caggcaacat ttttattaaa ttaagattgg aatgtcctat tggtcctgcc
     1801 aatgtcatat caagtcctat ttccgtatca atttcttcaa gaagagaata atttggattg
     1861 acacaagaaa ataaacaaca agacaataag cctgttaaac ttattctaac aagatgtttt
     1921 atcttcgtca tagtttaaat taattaattt ataatcgcaa aaataaacaa taatatgtat
     1981 atgtaaaaat tatttaacaa aaaacgtatc agggaaatta atgagataca atttttctgt
     2041 tgcacgtgta actgatgtat ataaccatct gacaaaagac ttatccattt tatcggcagt
     2101 aatcaatccc atatcaatat atacatgttt ccattgacca ccttgagctt tatggcaagt
     2161 aactgcgtat gccgattttg cttgtattgc attcagataa gggttatctc tcattttttt
     2221 gtaacgttca cgcttatttc tgatttcagc gtagtcttcc atgactgcat cgtataggcg
     2281 cttatttaat tcttgtaagt caaatggagt ttctgacaac aaagaatcct ctaaaaataa
     2341 tgcatctata tccatattgt aatctacaag atgtaaactt acttcacgaa atgagcaccc
     2401 ataaattgtt cttgctccgc gtgttctaat taattcagca acatcaccat ttgcaataaa
     2461 atcaagacca taagggagtc ctaccgtata gttgtttttt actatcataa gaagatctcc
     2521 atttgataaa acatcttccc tatataatat tcgcccacga atcccatttg catacatacc
     2581 tgcacgctta ttagatcttg ttatcacaat ggtttcatcc tctccaacct cacgatatga
     2641 gttttccatg cactctaaaa aattcatccc atctactctt atcacatcat taaactcctt
     2701 aaatgcaaca gcttgaggat tatctataag cattctaagg cgtgtggcgt tttgaaggat
     2761 accgctttct ttagcttgtc ttacaacctg ggttaaagta tattcagtaa tggttaatcc
     2821 gaaagtttct aaactacgca catccaatgc agggctctct tcacacgtaa caggcggtaa
     2881 ctgtgccgta tctcccaaca atagcaatct acaattttcg ccttggtaca caaattgtat
     2941 taaatcatca agaagattac ccgagccaaa aattttttcg ccggaatcag ctaacataga
     3001 tgcctcatct accacaaaaa gagtattttt ggccttattg taagctattt caaaagcacc
     3061 acctatttca ggtttacttt gtctatatat caatttatgt attgtatgag cctgaatgcc
     3121 tgcatataat gacaatactc tagcagcacg acctgtcggc gccgatagca caatcatttg
     3181 ctccatttct aaaaacgctt taaccaaagc accaatcaca gttgtttttc cggttccggc
     3241 aaatcctttc aataaaaaaa ggcttctttc ttcgcgattt gtaataaaaa ccgacagaag
     3301 atttatcaaa tcaacttgtt gcctagttgg ctcaaaacca aagtgagcga gtattttatc
     3361 tgcaacatat ttttctaaca tgattcaaag ataaatacat aaaaaaaatt gtgcaaataa
     3421 ataaaattct atacatttgc gaagttaaaa agcaaagata tggataaagt attaaaaatc
     3481 gttctagcat tagctgcaat atgccttaca tggctatgct accaaagcat caacactcca
     3541 attgagttta acgaacaacg cgattctcga gagaaaaaag tagtatctag attggttgac
     3601 attcgtcgtg cagaagtaga gtttaagaac atcaaaggag attacactac aaattttgac
     3661 acccttatag cttttgttaa aaattcaaaa gcaaaaatag ttttaaaaga aggtgttttg
     3721 acagataaac aattagaaga aggattaact gagcgcgaag ctgtaaaaca aggtattatc
     3781 aaacgtgata caacttacgt aagcatgctt gaatctttat ttggtaaaga ttatcctatc
     3841 gattctataa gatatatacc atattctgaa ccaagacaag aatttgaatt agcaaaagca
     3901 gaaatcgcaa caggtgctgc aggaatcaaa gttaaagtaa tggaagcaaa agccccattt
     3961 tctgtatatc taaatggttt ggataaacaa gaattagtaa atctaattga attaacagag
     4021 aaactagaaa gatatccagg acttaaattt ggtgacatcc aacaatcaaa caacaatgcc
     4081 ggaaactggg aataatccgg atttacatag tctatccatc cgcatctcta cggatggatt
     4141 ttctttttgt acaaacaacg aaaaaaaaga attatacttt acaaagaagg atcctgattt
     4201 taatcgttgt aaagctattt gcctttgtga gccctcacta cagaaagatt atggcaagac
     4261 gactatcttt tataatggca ttttcgcaac tttagttcca agcacaataa ccgattcaac
     4321 caatttagaa tcaatacttc tgtttaactt tcctgaaata tcatccgata aatttgatta
     4381 catatcaaat gctgtaatcg gatatgacat aacatatatt ttcgctattc caaaagcatt
     4441 acacaatttt ttagatgata ctttccctgc ttgcaaaatt atgcatgcaa attctataac
     4501 tctacaaata gccttgaagg attcaaaaaa gcaaggagaa aaattagttt gggcagatgt
     4561 atctaaatca gaagcatata taatattggc agaagaaggc aaattaaaat tcgccaaccg
     4621 atttgaattg accggagata aagatatcct atactactgt ggctgtgtat ttgagcagtt
     4681 tgctttatca caacacaacg ataaacttta tgtatcagga gatataacat gtatagacac
     4741 gctgcaaaat tattttgcta attgtattta taaagaaaca aatatatgcg tataataagc
     4801 ggtttatata aaggacgtca ttttacacca cctgcaaacc tacaagccag accaactact
     4861 gatttcgcta aagaaggatt attcaattta ttggacaatc gaatggattt aagtggtatc
     4921 aatgcattag atttattttc gggaacaggc agtatttgct acgaattagc atcaagagga
     4981 gccaattctg taacaggcat agaacaaaac tataagcacg ttgattacat aaaaaaaaca
     5041 gcatcagttt taaaaataaa ttgtttgcaa atattaaaag cagatgtatt caaattttta
     5101 gagacctgcc atacgcaata cgatttcgtt tttgcagatc ctccatacac attaagcaat
     5161 ctacaatcaa tacctgatct tatatttgct aagtc
//
LOCUS       DAOPID010000096         5191 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_21261_length_5191_cov_4.622858, whole genome shotgun sequence.
ACCESSION   DAOPID010000096 DAOPID010000000
VERSION     DAOPID010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21261_length_5191_cov_4.622858"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="PK641_04320"
     CDS             <1..573
                     /locus_tag="PK641_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HPG55608.1"
                     /translation="AVIMARVFKRFLFNGDDVPFVMELPPYRVPTIKSALHHMWEKSL
                     QYLKKMGTLILFASVVIWALGYFPLQKENETNQVHQEKSYIGKLGHFIEPAIKPLGFD
                     WKMGVGIIAGLPAKELVVSSLSVLYMGDDKSEDSPKLAESLQNDKAFNPIVAFGYMVF
                     ILLCFPCIATIVAIKNESGSWKWGIFSAAY"
     gene            624..800
                     /locus_tag="PK641_04325"
     CDS             624..800
                     /locus_tag="PK641_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55609.1"
                     /translation="MDINIEWIIVFIILVIAVVYIGIYIYRSFNRKPGCCDNSKTCDG
                     NPNNDACANCPLRK"
     gene            1268..3421
                     /gene="pnp"
                     /locus_tag="PK641_04330"
     CDS             1268..3421
                     /gene="pnp"
                     /locus_tag="PK641_04330"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPG55610.1"
                     /translation="MNVVTKTITLGDGRTITLETGKLAKQADGAVMVTMGKTMLLATV
                     CSAKDAVPGTDFMPLTVEYKEKFASNGKFPGGFTRREGRASDYEILTARLVDRALRPL
                     FPDDYHAETFVNVTMYSADGEDMPDALAGLAASAAIAVSDIPFNGPISEVRVARIDGK
                     FVINPTFEQLEKADLDIIVAATIDNIMMVEGEMKEVSETDLLNAIKVAHESIKVHCQV
                     QMELMEAAGKTQKRVYCHEVNDEDLRKDLHDKTFAKTYELAKSCNTDKHLREANFTAV
                     KDEYKAQFTEEELTEKASLIDRYYHDIEREAMRRAVLDEGLRLDGRKTTEIRPIWSEV
                     DYLPGPHGSAVFTRGETQSLSTVTLGTKQDEKMIDEALIKGNEHFLLHYNFPPFSTGE
                     AKAQRGIGRREIGHGNLACRALKNMIPENYPYVVRVVSDILESNGSSSMATVCAGCLA
                     LMDAGVPIKSPVSGIAMGLISENKGTNYAILSDILGDEDHLGDMDFKVTGTKNGITAT
                     QMDIKVDGLSFEILEKALKQARDGRMHILEKIQETLAEPRADLKPHAPRIVTMSVPKD
                     MIGAIIGPGGKIIQGMQAETGATISITEADDKGVVEIAGTNKQCIDAAVAKIKAIVAI
                     PEIGEIYDAKVKSIMPYGAFVEFMPGKEGLLHISEISWKRIETMEDSGLKENDIMSVK
                     LLEIDQKTGKFKLSHKVLTERPPRDPKKDETPKAE"
     gene            complement(3485..4144)
                     /gene="phoU"
                     /locus_tag="PK641_04335"
     CDS             complement(3485..4144)
                     /gene="phoU"
                     /locus_tag="PK641_04335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HPG55611.1"
                     /translation="MKQTEYEIRKVKDQYARMWYLVEKQVVSSFATVENFDRDAARAI
                     VMREKMVNAQELAIDDACERFLALFAPVAVDLRFVISMIKINNNLERIGDFAEGLAKF
                     CLNRQTQALSQELKESLRWHELTDTTLKMMQMAHESFETENSDKALSLMDLDAVVDEI
                     YISSTGVLADAIRANPKNAEEFMALLEVVRRIERMCDHFNNVAEDIVFYLDARELRHQ
                     S"
     gene            complement(4155..4529)
                     /locus_tag="PK641_04340"
                     /pseudo
     CDS             complement(4155..4529)
                     /locus_tag="PK641_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005788575.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(4534..4797)
                     /locus_tag="PK641_04345"
                     /pseudo
     CDS             complement(4534..4797)
                     /locus_tag="PK641_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005832302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(4815..>5191)
                     /locus_tag="PK641_04350"
     CDS             complement(4815..>5191)
                     /locus_tag="PK641_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55612.1"
                     /translation="SSGSGYMNGEATFSGSHDANAGDLYLRPFMGGYVYLVQNIVNSK
                     HSITVKYDCYDPNYMLSGTECLNIGDIKYQTLGIGYMFTLSKYLRFMAYVDLIWNENT
                     ALPDYTYNKRDNVLTVRAQYKF"
BASE COUNT         1718 a          966 c          907 g         1600 t
ORIGIN      
        1 gcagtaataa tggctagagt atttaaacgc tttttattta atggtgacga tgttccattt
       61 gtaatggaat tacctccgta cagagtacca actattaaat ctgcacttca ccacatgtgg
      121 gaaaaatctt tgcaatatct taaaaagatg ggaacactta ttttatttgc atccgtagtt
      181 atatgggctt taggatattt ccctctacaa aaagaaaacg aaactaatca agtgcatcaa
      241 gaaaaaagct atataggcaa attaggacat ttcatagagc cggcaataaa accattaggc
      301 ttcgattgga aaatgggtgt cggaataata gccggattgc ctgcaaaaga gcttgttgtt
      361 agttctctat ctgtgttata catgggtgat gataaatcag aagatagccc taaacttgcc
      421 gaatcattac aaaatgacaa agcatttaat ccaattgtag cttttggcta catggtattt
      481 atattattat gtttcccatg tatagcaaca attgtggcaa ttaaaaacga atccggctct
      541 tggaaatggg gaatattctc tgctgcatat taaacagcaa tcgcatggat aatggcattt
      601 ataatttttc aaattggtag cttatggata taaatattga atggataatt gtttttatta
      661 tacttgtaat agcagtagtt tacattggca tctacatata caggtcattt aatcgtaaac
      721 ccggatgttg cgataatagt aagacttgcg acggaaatcc caacaacgat gcgtgcgcaa
      781 actgcccact tcgtaaataa aaacatatcc ccgaaaataa gttttcgggg atattcaaat
      841 catagttttt ataaaaatat gttgtgtttt gtaagggcta agaaaaaccg taccgcaaat
      901 ttatgatcca aacttgttat tagtaggatt tgaggccccg gaactctttg taatccgccc
      961 aatcagctaa ctgatagcgg cacgccattg ataaacccga agtgactcgt ttcagaaata
     1021 gattgttaag tttcccaaaa tgttgcgata cggtattcaa agatacaatt ttttttcaaa
     1081 ttatcaaaca atctacgtat ttcttgtcat tatttttcaa atttattttt ttggttaaaa
     1141 ctttcataca aaattgttta ccaaaatcta acacgttttc ggttttgcag ttttattagg
     1201 aataataaaa aaatatagct aattttgtac gattttttaa agttaaaaac aagttaatgt
     1261 tatttgtatg aatgtagtaa caaaaaccat cacactcggt gatggaagaa ccattaccct
     1321 agaaacagga aagttagcaa aacaagctga cggagcagta atggtaacca tgggtaaaac
     1381 catgttattg gctacggttt gctcggccaa ggacgcagtt cccggtaccg attttatgcc
     1441 actaaccgta gagtataaag aaaaattcgc atcaaacgga aaatttccag gcggttttac
     1501 acgtcgtgaa ggacgtgcat cagactacga aattctgaca gcacgtttag tagaccgcgc
     1561 attacgtccc ctattccctg acgattatca cgctgaaaca tttgtaaatg ttacaatgta
     1621 ttctgcagac ggagaagata tgcctgatgc attagcaggt ttggctgcat cagcagcaat
     1681 cgcagtcagc gatataccat ttaacggacc aatttctgaa gttcgtgtag ctcgtataga
     1741 cggaaaattt gtaattaatc caacttttga gcaattagaa aaggctgatc tagacatcat
     1801 tgttgctgca actattgaca acattatgat ggtagaaggc gaaatgaaag aagtttctga
     1861 aacagacttg cttaatgcaa taaaagttgc acacgaatca ataaaagttc actgtcaagt
     1921 tcaaatggaa ttaatggaag ctgccggtaa aactcaaaaa agagtatact gtcatgaagt
     1981 taatgatgaa gacttgcgca aagacttaca cgataaaaca ttcgcaaaaa catacgaatt
     2041 agcaaaatct tgcaacactg acaaacactt acgcgaagca aattttactg ctgtaaaaga
     2101 cgaatataaa gcacaattta cagaagaaga gttaacagaa aaagcttctc tgatagaccg
     2161 ctactatcat gatattgaaa gagaggctat gcgtcgtgcc gtattggatg aaggtttacg
     2221 cttagatggt cgtaaaacaa ccgaaatccg ccctatttgg tctgaagtag attatctacc
     2281 cggacctcac ggttcagcag tatttactcg tggcgaaact caatcattaa gcacagtaac
     2341 cttaggaacg aagcaagatg agaaaatgat tgacgaagcc ttgataaaag gtaatgaaca
     2401 tttcttatta cactacaatt tccctccatt ttctaccggt gaagcaaaag cacaacgcgg
     2461 tattggtcgt cgtgagattg gacacggaaa cttagcttgc cgcgctctta agaatatgat
     2521 tcctgagaat tatccatatg tagtacgtgt agtttctgat atattggaat ctaatggttc
     2581 ttcatctatg gctacggtat gtgcaggatg cttggcactt atggatgccg gtgttcctat
     2641 caaatcacct gtatcaggta ttgcgatggg acttatctct gaaaacaaag gtacaaatta
     2701 tgcaatttta tctgatatct taggagatga agatcactta ggagatatgg actttaaagt
     2761 aaccggaact aagaatggta ttacagctac tcaaatggat ataaaggtag atggtttatc
     2821 attcgaaatt ttggaaaaag cactaaaaca agcacgcgat ggtcgtatgc atattcttga
     2881 aaaaatccaa gaaacattag cagaaccacg tgcagattta aaacctcacg ctccacgtat
     2941 tgttacaatg agtgttccaa aagacatgat tggtgcaatt ataggtcctg gaggaaaaat
     3001 tatacaaggt atgcaagctg aaaccggagc cacaatttct atcacagaag ctgatgacaa
     3061 aggtgtcgta gaaatagcag gaacaaacaa acaatgcatt gatgcagctg ttgctaaaat
     3121 caaggctatt gttgcaatcc ctgaaattgg tgaaatttat gatgctaaag taaaatctat
     3181 tatgccttat ggtgcattcg tagaatttat gcctggtaaa gaaggtttac ttcacatctc
     3241 tgagatcagt tggaaacgca ttgaaacaat ggaagattca ggtttgaaag agaatgatat
     3301 tatgtcggtc aaattacttg aaattgatca aaaaacagga aaattcaaac tatctcataa
     3361 agttttgaca gaacgtcctc cacgcgaccc taagaaagat gaaactccta aagcagagta
     3421 ataaaactta tagtctatat acaataatcc ccaaaagcaa aacttttggg gattattatt
     3481 ttaattaaga ttgatgacgt aattcgcgtg catctaaata gaaaacaata tcttctgcga
     3541 cattgttgaa atggtcacac atacgctcta ttctgcgaac tacttcaagc aaagccataa
     3601 attcttcagc atttttagga ttagctctta ttgcatcagc taaaacacct gtagatgaaa
     3661 tataaatttc atctactact gcatctaaat ccataagtga taaggcttta tccgaattct
     3721 cagtttcaaa agactcatga gccatttgca tcatttttaa agttgtgtct gtaagttcat
     3781 gccaccttaa tgattctttt agttcttgac tcaaggcttg tgtctgtcta tttaaacaga
     3841 actttgcaag tccttcggca aaatcaccta tgcgttcaag attattatta atcttaatca
     3901 ttgatataac aaaacgtaaa tcaactgcaa caggagcaaa taacgctaaa aagcgttcgc
     3961 aagcatcatc aattgccaat tcctgtgcat taaccatttt ttcacgcata acgattgccc
     4021 tcgcagcatc gcgatcaaaa ttttctacag ttgcaaaaga actgacaact tgcttctcga
     4081 ccaaatacca cattcgtgca tactgatctt ttactttcct aatttcatac tctgtttgtt
     4141 tcatagtctt aattttatcc gaatcttcct gtaatataat tttgtgtttc ctttttttcc
     4201 gggtttaaga aaatcctttt tgtatctcca tactcaataa gatttcccaa ataaaagaat
     4261 gccgttttat cagataccct tgtcgcttgt tgcatattgt gagtaacaat aattatggta
     4321 tattcatctt ttaaagaata aataagttct tcaattttag ctgttgatat tggatctaag
     4381 gctgatgccg gctcatccat caataatact gatggcctta cggctaatgc tctcgcaata
     4441 cataaacgtt gctgttgccc gccagacaga gcaaaagcag gcttatcaag ttggtcttta
     4501 acctcatccc acaatgcagc tcctctcaac ttgtaatttc atacgctcat caaactttat
     4561 tttacgagat gccatagcaa tatctgtatt ttcatcaatc aaagctgata ttcctacacc
     4621 tgaccctcca cctgttacag taactcttgc agatggattt tttttcataa aagcttctgc
     4681 ttgtttttgc gccaaaggca atactgtatc acttcctttc atcttttgag ccataagccc
     4741 attaacactc aatgctgttg ccacaataat ggcaataatt ttgtttggat ttttcatttt
     4801 cattaatttt tagtttaaaa tttatattga gctcgcaccg taagaacatt atctctttta
     4861 ttgtaagtat aatcgggcaa agcagtattt tcgttccaaa ttaagtcaac gtatgccata
     4921 aatcgcaagt attttgacaa agtaaacata tatcctattc ctaatgtttg atacttaata
     4981 tctccaatat ttaaacattc tgtcccggac aacatgtagt taggatcata acaatcatac
     5041 tttactgtta ttgaatgttt agagtttact atattttgca ctaaatacac atatccgccc
     5101 ataaatggcc ttaagtacaa atcaccggca tttgcatcgt gactaccact aaaagttgct
     5161 tccccattca tatatccgct tccactacta c
//
LOCUS       DAOPID010000097         5032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_22233_length_5032_cov_4.093229, whole genome shotgun sequence.
ACCESSION   DAOPID010000097 DAOPID010000000
VERSION     DAOPID010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 5032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5032
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22233_length_5032_cov_4.093229"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="PK641_04355"
     CDS             complement(<1..886)
                     /locus_tag="PK641_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55613.1"
                     /translation="MKNIDQTKNKLNTIFKRYGLILSLLFVTLGYSGSAWGGTYYLLM
                     GTDSNPSKMTSKGSQTADDEYEWTVTVQTSGNNYFGISTSDSYTNLLNASGHTITCNL
                     SPCTSSGQHYNFGGIDYYFKYFSSNQSSTVVIRYKASTTTYTISASSISVPVVRMGDI
                     PTPNPATNEAVTAYAYLAKTGCANVTKLRIYIGKDESPTKSSPYVEYTTGNPYSINNQ
                     YAITIPASKLNEIAGASSSFTAHIKATGFNSAGESELSDELTFSYQACPIGVEITPSS
                     GEMLVGSPTTFSTSVSGAG"
     gene            complement(977..>2807)
                     /locus_tag="PK641_04360"
     CDS             complement(977..>2807)
                     /locus_tag="PK641_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55614.1"
                     /translation="PSPFTFGTINRTSCTTANLAWSANAPALLVRYEGSSPSITNPTH
                     GQSYSSGNTIGAGTVVYSGTSDNYTATFDADKIYTFVIYSVSASGYLYSTSASTTLPK
                     GVPTVTTTAASSIDATSATLGGNVTAQGVGGAMSNRGIEWTLTVGGKTAGDKEIISTE
                     NATGAFSKEFTGLTGSTTYKYKAYATNNSCETSYGYGSEVEFTTSATCSTPSSGFAAS
                     GTYNSGNWTTRFANDYRTASLSTNQVADAYLWECTSANSSKVSIASNTSKTTNATFDL
                     PGEYTFRVGAKCTSDGGYTYSETVTVNVPYPDMGISGPFYDGNWDHPKAGTDGKFTRN
                     GLVYTKDFTVNTSNGGFVIIQAYKLGCESNSGWAGYQECNVDGKGNKINCSTLTTSTI
                     NTSSCGNFTQNAGLSNGQTARLTVTMTAYDSYTMSLGLACTPPTGGTATASASSVCSG
                     SGTTITLTGNTGTIQWQSSTNGTDYSNISGQTSSTLSTGNLTVKTWYRAVLTSDGCTS
                     NSSAAEVTIKTSPVLSAITGSATTTQYKPETYTASGSNLGTLTWAIVGAPAHANISAT
                     TGSSTVFKAKDGSYTLSVTATNDGCTDNETKGITVSTDSETCP"
     assembly_gap    2808..2817
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2818..3862)
                     /locus_tag="PK641_04365"
     CDS             complement(<2818..3862)
                     /locus_tag="PK641_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55615.1"
                     /translation="MKQELLHHVKNQSNVRNLSGFGLFNGMAKSLKFALLSLVFILLG
                     FVFGTQDVQAETYPEGQTVYVESSSNYSAVKLYAWGGTYSTPSWCGETMTSLGLIGSK
                     YYWSITLNGTFTNMIVCDNSCNKISGNTDGVASDGNTTNCITVTGDSYSWKLFNCTSY
                     SDCPSNVLTGTNVMFYINQSYGGTLGITNDSSSSCIANGTNLGNNNAYINMAKASVPD
                     TMSVSNNCGGWVGTGAFLRSTAYNAGNYFVTGASKSNTSATTASLSASSTSIAAGTSS
                     INITSTAGSVNSSFGKKLAIQYYISTTKYGCDFANTSGVATTINTSSLSQGTYKIKSV
                     LTDGTIYYLGDSIN"
BASE COUNT         1534 a          975 c          854 g         1659 t
ORIGIN      
        1 caccggcacc tgaaactgag gtgctgaatg tagtgggcga tccgacaagc atctcacctg
       61 aacttggagt tatctcaact ccaatcggac atgcttggta agagaatgta agttcgtctg
      121 ataattcaga ttcgcctgcg ctattgaaac ctgtagcctt aatgtgagcg gtaaaacttg
      181 aggacgcacc tgctatctcg ttcagttttg aggcaggtat tgttatggca tattgattat
      241 taattgagta aggatttcct gttgtatatt ctacataagg tgatgatttt gtaggtgact
      301 cgtctttacc tatatatata cgtaattttg ttacgttggc gcaacctgtt tttgctaaat
      361 atgcgtatgc tgttacggct tcatttgtgg caggattagg agttggtata tctcccatac
      421 gtacaacagg tactgaaata ctacttgctg aaatggtgta agtagtagtt gaagctttat
      481 accttattac tactgttgat gattgattag aactaaaata cttgaaataa taatctatac
      541 caccaaaatt ataatgttga ccactggagg tacatggtga aagattacac gtaatcgtat
      601 gaccagatgc attcaacaaa ttggtatacg aatctgatgt tgaaatacca aaataattat
      661 ttcctgatgt ttgcacagtc actgtccatt catactcatc atcagctgtt tgtgaacctt
      721 tcgaggtcat tttagacgga ttgctatctg tacccatcaa caaatagtat gttccacccc
      781 atgcgcttcc gctatatcct aatgttacaa acagtaatga caaaatcaaa ccataccttt
      841 taaatatggt atttagtttg ttttttgttt ggtcgatgtt tttcatgtta taatcgtttt
      901 aatttatttc ttttatttta ttttttttgc cactgccgta tgcctcaaca gagacatacg
      961 acaaaggcaa actctattat ggacatgttt cgctgtctgt acttactgtt attcccttag
     1021 tttcattatc ggtacaacca tcattagtag ctgttacact taatgtatat gaaccatcct
     1081 tagctttgaa tactgtgcta cttccggtag ttgctgagat atttgcgtgc gccggcgcac
     1141 caactatcgc ccaagtaaga gttcccaaat tgctacctga tgcggtatat gtttcgggtt
     1201 tatactgggt tgttgttgca gatccagtta ttgctgataa tacaggagat gttttaatag
     1261 ttacttctgc tgccgatgaa tttgaagtac aaccatcaga tgttaataca gcacgatacc
     1321 atgtttttac agttaaattt cctgttgata atgttgatga tgtttgtcca ctaatattag
     1381 agtaatccgt accatttgtt gaagattgcc attgaatagt accggtatta ccagtaagtg
     1441 ttattgttgt accactacct gagcaaacag aacttgctga tgcggttgca gtaccaccag
     1501 tagggggtgt gcaagctaat cccaatgaca tagtatacga atcgtaagca gtcattgtga
     1561 cagttaatcg agctgtttga ccatttgaca aaccagcatt ttgtgtaaaa ttaccacaac
     1621 tgcttgtatt aatagtactt gtagttaaag tagaacaatt tattttatta cctttgccgt
     1681 ctacattaca ttcctgatat ccagcccatc cactattaga ctcacaacct agtttatatg
     1741 cttggattat aacaaatcct ccattagatg tatttacagt aaaatctttt gtgtaaacca
     1801 aaccattgcg agtaaactta ccatcagtac cggcttttgg atgatcccaa tttccatcat
     1861 agaaaggtcc acttataccc atatccggat aagggacatt aaccgtgaca gtttcagaat
     1921 atgtatatcc accatccgaa gtacattttg cacccactct aaaagtatac tcaccaggca
     1981 aatcgaatgt tgcgttggta gtctttgaag tgttgcttgc tattgatact ttactgctat
     2041 ttgcgcttgt acattcccac aaataagcat ctgctacttg gtttgtactt aatgatgcag
     2101 tacggtaatc attagcaaaa cgtgttgtcc aatttcctga attataagtc ccgcttgccg
     2161 caaaaccaga tgaaggagtg ctgcaagttg cagaagttgt aaattcaact tcagaaccat
     2221 aaccataaga agtttcgcaa gaattatttg tagcgtatgc tttatatttg taagttgtac
     2281 tacctgttaa acctgtaaac tcttttgaga aagcaccggt agcattttca gtacttataa
     2341 tttctttatc accggctgtt ttgcctccaa cagtaagagt ccactctata ccacgatttg
     2401 acattgcacc accaacacct tgtgctgtaa cattaccacc caaagtagca ctagtagcat
     2461 ctatacttga agcagcagta gtcgttacag taggaactcc tttaggcaaa gttgttgatg
     2521 ccgaggttga atacaaatat cctgatgcag acacactgta tatgacaaac gtgtatattt
     2581 tatctgcatc aaaagttgct gtataattat ctgatgttcc gctgtataca acagttcctg
     2641 ctccaatagt atttccggat gaataacttt gaccatgagt agggttggtt atggatggac
     2701 tacttccctc ataacgaacc aacaaagctg gtgcatttgc agaccatgcc agatttgcgg
     2761 tagtacaact agtcctattg atagtgccga aagtaaaagg tgaaggannn nnnnnnnaat
     2821 taatcgaatc acctagataa tatattgttc cgtcagttaa aactgatttt attttatatg
     2881 ttccttgcga taaacttgag gtatttattg ttgttgctac tcctgatgtg ttagcaaaat
     2941 cacaaccata ttttgttgta ctaatatagt attgtattgc caacttttta ccaaaagaag
     3001 aatttacact gccagctgtt gaggtaatgt taattgaaga tgtacctgcg gctatcgatg
     3061 tagaagaagc tgaaagtgat gcagtggttg cacttgtgtt agatttggaa gcgcccgtta
     3121 caaaataatt accggcatta tatgctgttg accttaaaaa tgcacctgtt cctacccaac
     3181 caccgcaatt attagataca ctcattgtat cgggaactga tgcctttgcc atatttatgt
     3241 aagcattatt attgcctaag ttggttccat ttgcaataca acttgaagaa gaatcatttg
     3301 taattcctaa tgttcctcca taactttggt ttatataaaa cataacattg gttcctgtta
     3361 aaacgtttga aggacaatct gaatagcttg tacaattaaa taacttccaa gagtaactgt
     3421 ctccagtaac agttatacaa tttgttgtat ttccgtcact agctacacca tcagtatttc
     3481 cactaatctt gttacaacta ttatcgcaaa caatcatatt tgtaaatgta ccattcaatg
     3541 ttattgacca ataatattta cttccgatta aacccaatga agtcatagtt tctccgcacc
     3601 aacttggagt tgaatacgtt ccgccccagg cgtaaagttt tactgcacta tagttactgg
     3661 aagattccac atacactgtt tgaccctccg gatacgtttc agcttgcaca tcttgtgtac
     3721 caaatacaaa tcctaataat ataaacacca atgacaaaag agcaaatttt aaagactttg
     3781 ccatcccatt aaacaaacca aagccggata aattccgcac attagattga tttttcacat
     3841 ggtgtaatag ttcttgtttc atgttcttgt tttttatttg ttgttttgtt atttatccga
     3901 ataatatata ctgtactttt tagcacaaat gaattttaaa tttatcaata cgtttccttc
     3961 aaacctaaaa actaaattgt tatgagaaaa atggttttaa aatactatat caaaaaacca
     4021 tatacttttt taaaaacttg ttaaaacaac gtcgaactaa aaatatttca attttaccca
     4081 gttaaatatt gttaatgttc aatttttgtg actcaatttt aataattggg tccttttttt
     4141 tgatagctgc attccaaaac acagcaatgt caaagagtgg taaaaaccta ttagtcaaca
     4201 aaatacacaa aaatcaaaag gcttgtcgcc acaatacaat gctaatatac aaacaatttt
     4261 tcaattgtac aacacccatt tatttccaca aaatccggca cttatgacgc atattatagt
     4321 gcagttttat aacataattt taaaaattaa ataaaaatgc ctattcatcg gaagaaatag
     4381 ctatttaatt gataataaaa ggattaaaaa tcatttatta tacatcaatt tgtgaaatta
     4441 gtttatagtt aatttcacaa gcaataatac tacattttgt taattcacaa cacacaattg
     4501 cgggagaggg gtgaggggtt aagggtgagg ggtgtgtagt gtgtaatcaa tatatgcact
     4561 tcgtgcctac cgcggcttcg ccccggaccc cagttcattt tctttgtctt gtccaaagaa
     4621 aacgaaccaa aagaaaagcg ctttttgggg ttccgcttgc aaattattgc taaaaattgc
     4681 cttctgcaat ctaaaactgc tatgcgccct acgggacgca gtttttgacg attgcatcag
     4741 tataattttt tatacgcaat aattttatag gcggtcctaa cgagtagtgc tcgtcacacc
     4801 ccttctcccc taacccctca ccctacacat tacacactac acattacaca ttccacacta
     4861 cacattacac gattccacaa tacgcgcccc tcacattaca cacgatactg catatagtgg
     4921 cacattagtt attatttctt caacacaata ggctgacatg gaaatgcgat aagccaaaga
     4981 ggacgagttt tcacgtgtat attgacgcaa actttttgca tttaggttta tt
//
LOCUS       DAOPID010000098         5018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_22333_length_5018_cov_3.914568, whole genome shotgun sequence.
ACCESSION   DAOPID010000098 DAOPID010000000
VERSION     DAOPID010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22333_length_5018_cov_3.914568"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="PK641_04370"
     CDS             complement(<1..616)
                     /locus_tag="PK641_04370"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55616.1"
                     /translation="MKNLKIYFFIILTCSSILSCTSCKNSEPIDNKYKYAVNIASVEN
                     GSVNVLCDGQVVLSGDSLYAGSSITVTAVPNENYELSSMNVSNPFELSKDTTIRVVFA
                     KNSFAFSVAPFEGGTVKVSYRGTEYQPGSIFIKNTYLIVEFVADDDHYFTGSNIANGT
                     GVRDSFKLVSDTIIRPTFETITVVSPSDYHNICSYNIRLYTTDDK"
     gene            complement(631..2430)
                     /locus_tag="PK641_04375"
     CDS             complement(631..2430)
                     /locus_tag="PK641_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007486641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPG55617.1"
                     /translation="MVSYLQIEGLTKSFGNLVLYEKIDFDLSEGQRVAIVAKNGAGKS
                     TLLNIIAGNDTADEGKISFKRDLRISFLEQDPKYNLDSTVFDAFMSVENECKIRHKED
                     EWEYETRAKQILTKLQVGEFDKKISQLSGGQLKRLALANALISEPDLLILDEPTNHLD
                     LEMVEWLEEYLNKSNMTLLMVTHDRYFLDRVCSDIVEIDQKKIYRYKGNYSYYLEKHQ
                     DRVDNQNAELAKANNLYRKELDWMRRQPQARGTKAKGRVDAFYNLQDKVSVNNKEENI
                     RLEVKSSYLGSKIFEAKYICKAYENLVILKDFYYNFTRYEKLGIVGKNGTGKSTFLKL
                     LLGIQKPDSGKFDIGETVRFGYYSQDGLAFNDQMKVIDVVRDIAEEIDLGGGRKMSAS
                     QFLQHFLFTPETQYNYVYKLSGGEKRRLYLCTVLMKNPNFLVLDEPTNDLDIMTLNIL
                     EEYLRDFKGCVIVVSHDRYFMDKVVDHLLVFKGNAEIKDFPGNYTQFRYWRDETEKEL
                     RQQEKVKESVSKPVPRVERKRRLTYAEQKEYESLSKEIEDLDNEKKNLESSFLNNDLT
                     PDQLMLNSERIALIIKLLDEKEMRWLELEDLNN"
     gene            2487..4676
                     /locus_tag="PK641_04380"
     CDS             2487..4676
                     /locus_tag="PK641_04380"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HPG55618.1"
                     /translation="MRKYAISLLFLLSSQLLSFADEGMWILPNIPDSVSESFEELGCE
                     LEIKDIYHERTYSLKDQTAYLSNGYTATLLSESGLLITNYNAITKYISEKDSNIILNG
                     FYASEKYNEIPLGNLHVFFLKSTENVTWRISSKFLDSDNESVRKNKTDSISRLICNEK
                     VLPAGCFAQVKKTSNEEYYLYIYERYSDLRLSYIPPKYIAEYGNKKDKWTASRHNADF
                     ALIRIYAARDGQPAYYSDTNKHAEFSGCAKIAVDPYKEDDFVFALGYPNSSERNIRAA
                     DIYERIMIKNRISKQIMSRLQDMKYRDYQDKIDYISRLETYIDKQNIIKKKRAKEYSF
                     IKWAANNANFNIALRYSNVIPHLNSYYKKRESAYSQLLYLKEFSNQIDLLMVGSMMIE
                     LNDNNQDDIFYEISRFYERYNPAIEKQVLLVALNTWKEKCESAYLPDIFNFIQEKYKG
                     DTEKYLDDLFKKSFITEEIRFNKYLDRPTEKQFESDMLIVLCKSILNAQWQAYSAYMI
                     DNDKIERCNRLYKEGVNIQHPSNMAMPDANYTLRMSVGKIASYSPSDGYTINYATTFN
                     GLFEKSYIDQYEDKIDYKLKALRNKGKDLRLNFMTTCDMPPSNTGEGVYNANGDLLGI
                     LSSGNSETICSEYSYNEQYQRTMALDINYVIFLLKEYSKANKLIEELHFREAEKYVNI
                     EYIEDTKKPEPAETIIYTSEDEKAMVLDNVEEKTTPDKNDESTESEN"
     gene            4648..>5018
                     /locus_tag="PK641_04385"
     CDS             4648..>5018
                     /locus_tag="PK641_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HPG55619.1"
                     /translation="MMSLQKAKIKIVADKNIPFLRGIFEDVADIEYLAAPDIDKFAIQ
                     DADALIIRTRTKCDENLLQGSSVKYIATATIGYDHIDTEYCKANKIEWSNAPGCNANS
                     VVNYINAVLKFTESFPTQKTIG"
BASE COUNT         1751 a          870 c          773 g         1624 t
ORIGIN      
        1 ccttatcatc agtagtatac aagcgaatat tataactaca aatattatga taatctgatg
       61 gagaaacaac tgttatcgtt tcaaaagtag gtcttattat tgtatctgat acaagtttaa
      121 aagaatctct tacacctgta ccattagcta tatttgagcc tgtaaaataa tggtcatcat
      181 ctgctacaaa ctcgacaata agatatgtgt ttttaataaa tatactgccc ggttgatact
      241 ctgtaccacg ataactaacc ttaactgtgc caccttcaaa tggtgcaacc gagaatgcga
      301 aagaattttt tgcaaaaaca actcttattg tagtatcttt tgacaattca aaaggattac
      361 ttacgttcat tgatgacaat tcataatttt catttggtac tgcagtaaca gtaatagacg
      421 aacctgcata caacgaatcg ccgcttaata ctacttgacc atcacacaaa acatttacag
      481 atccattttc tacagaagct atgttaactg catatttgta tttattatca atcggttcgc
      541 tgtttttgca agatgtacaa ctcaaaattg acgaacatgt caatattata aagaagtaga
      601 ttttaaggtt tttcataatg tataaggtta ttaattattt aagtcttcta attcgagcca
      661 acgcatctct ttttcgtcta ataattttat tatcagagct attcgttctg agtttaacat
      721 tagttgatca ggggttaaat cattatttaa gaaagaggat tccaagtttt ttttctcatt
      781 atccaagtct tcaatttctt tgctcaatga ctcatattct ttttgttcag cataagttaa
      841 acgacgtttg cgttctacac gaggaaccgg tttgctgaca gattctttta ctttttcttg
      901 ttggcgtaat tctttttcag tttcatctcg ccagtatcta aattgggtat aattgcccgg
      961 aaaatcttta atttctgcgt ttcctttaaa tactaataaa tgatctacaa ccttatccat
     1021 aaaataacga tcgtgagata caactataac acagccttta aaatcgcgca aatattcttc
     1081 aagtatgttt aatgtcatga tatccaaatc attagtaggt tcatcaagta ctaagaaatt
     1141 cggattcttc attaaaaccg tacataaata taatctacgt ttttctcctc cactcaattt
     1201 atatacataa ttatactgtg tttcgggagt aaacaaaaaa tgctgcaaaa actgagaagc
     1261 actcattttg cgtccaccac ctaagtcaat ttcttctgct atatcacgga caacatcaat
     1321 aactttcatt tgatcgttaa aagctaaacc atcttgacta taatagccaa atcgaactgt
     1381 ttcgccaata tcaaatttac cgctatcggg cttctgaatt cctaataaca attttaaaaa
     1441 tgttgatttt cctgtaccat ttttacctac aataccaagc ttttcataac gagtaaaatt
     1501 atagtaaaaa tcctttagta taaccagatt ttcgtatgct ttacagatgt atttggcttc
     1561 aaagattttt gaaccaagat atgaagattt tacttctaaa cgaatattct cttctttatt
     1621 attcacagaa accttgtcct gtaaattgta gaaagcatca accctacctt tagcttttgt
     1681 tcctcgggct tgcggctgac gacgcatcca atctaattct ttacgatata aattatttgc
     1741 ctttgccaac tctgcatttt ggttatcaac acgatcttga tgtttttcaa gatagtagct
     1801 gtaatttcct ttgtatctat aaattttttt ttgatctatt tccacaatgt ctgaacagac
     1861 cctatctaaa aaatacctat catgtgttac catgagcaag gtcatattgg atttattcag
     1921 atattcttcc aaccattcaa ccatttctaa atcaagatgg tttgtgggtt cgtctaatat
     1981 aagtaaatcc ggttcgctga taagtgcatt agctaaagcc aagcgtttca actgacctcc
     2041 tgataattgc gatatttttt tatcaaattc gcctacttgt aactttgtca gaatttgttt
     2101 tgcccgtgtc tcatattccc actcatcttc tttatgacga attttacatt cattttcaac
     2161 actcataaag gcatcaaata ccgtgctgtc aagattgtat tttggatctt gctccaaaaa
     2221 tgaaatacgc aaatcacgtt tgaacgagat ttttccctca tcagcagtgt catttccggc
     2281 aataatatta agtagggtac tttttccggc accatttttt gcaactattg caacacgctg
     2341 cccttcagat aaatcaaaat cgattttctc atacaaaact aaattgccaa aacttttagt
     2401 taaaccttct atttgtagat aactaaccat aataatgggc aaaagtacaa aaaaatcatt
     2461 atttttgcat ctcatttata tgttgtatga gaaaatacgc catttcatta ttgtttttgt
     2521 tatcatcgca acttctttca tttgcagatg aagggatgtg gatattgcca aatatacccg
     2581 actccgtatc tgaaagtttt gaggaattag gttgcgaatt ggaaatcaaa gacatatatc
     2641 acgaaagaac ctattctttg aaagaccaaa ctgcttatct gtcaaatggt tacactgcta
     2701 cacttctatc agaatccggt ttgcttatta caaattataa tgccattact aagtacatat
     2761 ccgagaaaga ttctaacatt atactcaatg gtttttacgc atctgaaaaa tataacgaaa
     2821 ttccactagg taacctgcat gtattctttt tgaaaagcac tgagaatgta acatggcgaa
     2881 ttagttctaa atttttagat agtgataacg aaagtgtcag aaagaacaaa accgattcaa
     2941 tatcaagatt gatttgtaat gagaaagttc ttcctgccgg atgctttgct caggtaaaga
     3001 agacatctaa tgaagaatac tatttatata tttacgaaag atattctgat ttaagattga
     3061 gctatattcc acctaaatat attgcagaat atggcaataa aaaggacaaa tggacagcga
     3121 gtagacacaa tgccgacttc gctctaattc gtatatatgc agccagagac ggacagccgg
     3181 catattatag tgataccaac aaacatgcag aattttcagg atgcgcaaaa atagcagttg
     3241 acccatacaa ggaagatgat tttgtgtttg ctttaggata tcctaatagc tctgagcgta
     3301 atattagagc tgctgatatt tacgaacgca taatgattaa aaatagaata agtaaacaaa
     3361 taatgtctcg gttgcaagat atgaaatatc gcgattacca agacaaaatt gactatataa
     3421 gcagactaga aacgtacatt gacaaacaaa atattatcaa gaaaaaacga gccaaagagt
     3481 atagttttat taaatgggca gcaaataatg ctaatttcaa tattgcgcta agatactcaa
     3541 acgttattcc acatctaaac tcatattaca agaagcgaga atccgcttat tctcaattac
     3601 tttatttaaa agaattttct aatcaaattg atttactgat ggttggaagt atgatgattg
     3661 aacttaatga caacaatcag gatgatattt tttatgaaat aagccgtttt tacgaaaggt
     3721 ataatccggc aatagaaaaa caagttttac tagttgcttt aaacacctgg aaagaaaaat
     3781 gcgaatcggc atatctacct gatattttta attttataca agaaaaatac aaaggtgaca
     3841 cagaaaaata cttagatgat ttattcaaaa aatctttcat tactgaagaa attcgtttca
     3901 acaaatattt agacaggccc acagaaaagc aattcgaatc agatatgcta attgtactat
     3961 gcaagagcat cttgaatgca caatggcaag cctactctgc ctatatgatt gataatgata
     4021 aaattgagag atgtaataga ttatacaaag aaggtgttaa tattcaacat ccctcaaaca
     4081 tggctatgcc tgatgctaat tacactcttc gtatgagtgt agggaaaata gcttcatata
     4141 gtccatcaga cggatatacc attaattatg ctactacatt caatggacta tttgaaaaaa
     4201 gttatataga tcaatatgaa gacaaaattg actacaaact gaaagcttta cgaaataaag
     4261 gcaaagatct gcgcttaaat tttatgacaa cttgtgatat gccgccatca aataccggag
     4321 aaggtgtata taatgctaat ggcgatttac taggtatatt gtcaagcgga aattctgaaa
     4381 caatatgcag cgaatattct tacaatgaac aatatcaacg taccatggca ttagatatta
     4441 attatgtaat atttttacta aaagaatatt ctaaggctaa taaattgata gaagaattac
     4501 atttcagaga agcagaaaaa tacgtaaaca ttgagtatat agaagatacc aaaaaacctg
     4561 aacctgcaga aacaataata tatacttcgg aagatgaaaa ggctatggta ttagataatg
     4621 ttgaagaaaa aactacaccg gacaagaatg atgagtctac agaaagcgaa aattaaaatc
     4681 gttgcagaca aaaacattcc gtttttacga ggcatatttg aagatgtagc agatattgaa
     4741 tacttagctg cacccgatat tgataaattt gcaatacaag atgctgatgc tctaattatt
     4801 agaacgcgga cgaaatgtga tgagaacctg ctacagggca gttctgttaa atacattgca
     4861 actgctacaa ttggatacga ccatattgat actgaatatt gtaaggcaaa taaaatagaa
     4921 tggtcaaatg ctccgggttg caatgccaat tctgttgtca attatattaa tgccgtactt
     4981 aaatttacag aatcgttccc tacacaaaag actatagg
//
LOCUS       DAOPID010000099         4967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_22644_length_4967_cov_3.202769, whole genome shotgun sequence.
ACCESSION   DAOPID010000099 DAOPID010000000
VERSION     DAOPID010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4967
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22644_length_4967_cov_3.202769"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3040)
                     /locus_tag="PK641_04390"
     CDS             complement(<1..3040)
                     /locus_tag="PK641_04390"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPG55620.1"
                     /translation="MKKYFLSFLFSLLCLSINAQPAYLAGSSTGFNELNSSPSGSNMT
                     VETRLNTTVAVKTCSAKADYSAWFKFTSSNNSWEPQWCASGSEKTIATNAYLSSTAEY
                     VTGQNLKVDVLSGKYYTFIVGQNATTNNDLSVLLSYEAPKTISSITNATPAENTDIQV
                     SATLSSAIGVTSGDGAQYVRLSYTIDNISYVTVDMNGSGSVYTANIPASVNTDGATVK
                     YFVFVTPISNLTPSTANFDYLYFTKSNTNTYCIGCVSNPITVKAKRPSSWTNDMYIFY
                     WGGEISSPSWSDAELMESIGNDWYSYTFDPTITSTNIIFRNINDGTNDPVNQSVDINT
                     VSVDVCYELSQSGSSAATYSEVDCSNINSDVDAPEIRAHRNTVNENDFSDFASLYVYS
                     NGNLNTTDFTGATKYLWQKSTDGSVWSTFEEGRKNNINNIRPVTAAYYRCLVSRYCVS
                     TSKDSTLISNQIQVTNTSGSTNTISSKLPIIIVRTDVKDFPVQPGGNGFLSSNEALAA
                     GKEKISVDVKIIWDETSTDDQTNTYVGDDVTNLSRLYYDKKARMNYRGASSMNYDRKN
                     YAFVVGDDSCTVSGTWVKDKKKMFNLNETKDKDWILYAAYTDNTKMRNLLSMNLYEDM
                     TGTWNSHGRYVRLYVNGEDKGIYIFMEKNKQESSRVQVAEDGYIFKFDKTDIVDRVSS
                     TEGGRCTFTTDKTGWQNIGTYGLIIDQAFEVVYPEWDEADFTQEVWNERMLALKNRIN
                     DFETALAAGNYASARNYIDYESWADNFIINEYTKNVDGYRISQFFNMSSATGLIKAAP
                     LWDMELGFNNSSAYGASEKGCTSTSGLMAENSAVHSDSFTIPFWWTGSDKAYKGYGLL
                     NDDCFKAKVKERWLIHTAAGGALNESNVQSEINKLASSLNVSAPSVITDWVNTTSGRP
                     AGLNSIISAYPTYISSASMSDVSNVALGSAAQMAVSVSGTGNYTYAWYFCTTNSINDS
                     DWTLLTDNGSATYSIASAKTTDDGFYRCVVTSQTCDCINKS"
     gene            complement(3077..4312)
                     /locus_tag="PK641_04395"
     CDS             complement(3077..4312)
                     /locus_tag="PK641_04395"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HPG55621.1"
                     /translation="MIITISLPAFSARNLYVVKSDLSETIYNTTDIDSITFDDPQIDD
                     NYIISLHDGENVASFNVSELNKIYLGPYFDEDDINNNEFGTTYTFTYKSSSVTVFPTP
                     TEGITVEQNGTNIVVRSELEGINYVLKSSMYRTGSFKLYSAHKSQLTLNGINLGSSNG
                     SAINIQTGKRTFVVIADGTQNYITDSAVYPVETEYSEDQKAAIFSEGELIFSGKGSLN
                     VSSLAKHGIASDDYVSIYDGNIVVSNAVKDGLKSKQKFVMKGGSLNITALDDGLDCTE
                     GYIKIYDGDINITSADKGLVASYDSADVVANFEVFDPSVNTNITINGGTMSITTGVNA
                     SIDEQKAHGIQTSSDVIINSGIININTNVVNKSDGINCNGSFVVNGGSITIEANDDCY
                     PIPIFNAGTLSCDGAIYVP"
     gene            4494..>4967
                     /locus_tag="PK641_04400"
     CDS             4494..>4967
                     /locus_tag="PK641_04400"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HPG55622.1"
                     /translation="MITTKTIADLLHEISSRSNTKVGFVPTMGALHQGHISLVKKCVK
                     ENDICVVSIFVNPTQFNDKNDLAKYPRTPDADTVLLEEAGCDILFMPSVSEMYPTEDT
                     RKFDFGNIEKVMEGKFRPGHFNGVAQIVSKLFYAVKPNKAYFGEKDFQQVAIIKAM"
BASE COUNT         1643 a          952 c          720 g         1652 t
ORIGIN      
        1 ctgacttatt aatacaatcg caagtttgtg aagtaacaac acaacgataa aatccgtcat
       61 cagtagtttt agcgctcgct attgaataag tagcagatcc attatcagtc aacaaagtcc
      121 aatcagaatc gttaattgaa tttgttgtac aaaaatacca tgcatatgta taattacctg
      181 ttccggaaac gcttactgcc atttgtgctg cacttcccaa tgctacattt gacacatcac
      241 tcatagatgc agaagaaatg tatgtaggat atgctgaaat aatgctattt aaacctgcag
      301 gacgtcctga agtcgtattc acccagtctg taattacaga aggcgcagaa acattcaaag
      361 acgatgctaa tttattaatc tcactttgaa cattactttc atttaatgca ccacccgctg
      421 ctgtatgtat taaccaacgc tcttttactt ttgctttaaa acaatcatca ttgagtaatc
      481 cgtaaccttt ataagcttta tctgaacctg tccaccaaaa tgggattgta aaagaatctg
      541 aatggacagc agaattctct gccattaaac cgcttgttga agtacatcct ttttcacttg
      601 caccataagc tgatgaatta ttgaatccca attccatatc ccacaaagga gctgccttaa
      661 ttagtcctgt agcagaactc atgttaaaga attgagatat tctatatcca tcaacatttt
      721 tagtgtattc atttataata aaattatctg cccacgactc ataatcaata taattacggg
      781 cgcttgcata atttccggca gccagagcag tttcaaaatc attgatgcga tttttaagtg
      841 ctaacatacg ttcattccaa acttcttgcg taaaatctgc ctcatcccat tccgggtaca
      901 ccacttcaaa agcttggtca ataattaaac cataagtacc aatattttgc caacccgttt
      961 tatcggtagt gaaagtacat ctaccccctt ctgtggaact tactctatct acaatatcag
     1021 ttttatcaaa tttaaatata tatccatctt cggcaacctg tacacgactt gactcctgtt
     1081 tatttttctc cataaagata taaataccct tatcttcacc attaacatac aagcgaacat
     1141 atctgccatg gctattccat gtgccggtca tatcttcata tagattcatt gacagcaaat
     1201 tacgcatctt agtattatca gtatatgctg catataatat ccaatcttta tcttttgttt
     1261 catttagatt gaacattttt ttcttatctt ttacccacgt tccggataca gtacagctat
     1321 catctccaac aacaaatgca tagttttttc tatcataatt catacttgat gcaccacgat
     1381 aattcattct cgctttctta tcatagtata aacgagacaa attcgttaca tcatctccta
     1441 cataagtgtt agtttgatca tctgtcgatg tttcatccca aataatttta acatcaacgc
     1501 ttattttttc tttaccggca gcaagagctt cgtttgatga taaaaatcca ttgcctccgg
     1561 gttgtacagg aaagtctttt acatctgtac gaactattat gatcggcaat tttgaagaaa
     1621 ttgtatttgt tgatccgctt gtattggtaa cttgaatttg attagaaatt aaggttgaat
     1681 ctttagatgt tgaaacacaa taccttgata ctaagcaacg ataataagct gctgttacag
     1741 gacgaatatt attaatgtta ttctttctac cttcttcgaa agttgaccaa acagaaccgt
     1801 cagttgattt ctgccataaa tattttgtcg ctcctgtaaa atctgttgta ttcaaatttc
     1861 catttgaata cacatataat gaagcaaaat ctgagaaatc attttcattt actgtgttcc
     1921 tatgcgccct aatttcagga gcatcaacat ccgaatttat atttgaacaa tcaacttctg
     1981 agtacgttgc agcagaagaa ccgctctgac tcaattcata acatacatct acagaaactg
     2041 tatttatatc aactgattga tttacaggat cattagtacc atcattgata tttctaaata
     2101 taatgttagt actagtaatg gtaggatcga atgtatatga ataccaatca ttacctatac
     2161 tttccattaa ctcagcatca ctccatgatg gtgatgaaat ctcgccaccc caataaaaaa
     2221 tatacatatc atttgtccat gaagatggcc ttttagcttt aactgtaatt ggatttgaca
     2281 cacatcctat acaatatgta tttgtatttg attttgtaaa atacaaataa tcaaaatttg
     2341 cggtagatgg tgtcaagttt gagatcggcg taacaaatac aaaatactta acagttgcac
     2401 cgtcagtatt aactgaagcc ggaatatttg ctgtatatac tgaaccggag ccgttcatat
     2461 caactgttac atatgatata ttatctattg tgtaagacag acgaacatat tgagctccgt
     2521 caccggatgt aactcctata gcacttgata atgttgcaga aacctgtata tcagtatttt
     2581 cagcaggagt agcatttgtt attgaagaaa tcgttttagg tgcttcgtat gacaataaga
     2641 ctgataaatc attattggta gtagcattct gtcctacaat aaatgtatag tattttcccg
     2701 acaatacatc aaccttcaaa ttttgtccgg taacatattc tgcagtagat gacaaataag
     2761 catttgttgc tatggttttt tctgaacctg atgcacacca ttgtggttcc cacgaattat
     2821 tagaacttgt aaatttaaac caagccgagt aatctgcttt tgctgaacat gttttaacag
     2881 caacagttgt attcagtctg gtttcaacag tcatgtttga tccggaaggc gaactattta
     2941 attcgttaaa gccggtacta cttcctgcta aatacgcagg ttgcgcatta attgaaagac
     3001 aaagaagcga aaatagaaat gataaaaaat attttttcat aatgttaagg ttttttcttc
     3061 tttatataat tgtaaatcat ggcacataaa tagcaccatc acaagacaat gtacccgcat
     3121 taaatatcgg aatcggataa caatcatcat tagcttcaat cgttatactt ccgccattta
     3181 caacaaaact accgttacaa ttaataccat ctgatttatt tactacattg gtattaatat
     3241 tgattattcc actattaata ataacatctg atgaagtttg aattccatga gctttttgtt
     3301 catcaatcga agcgttcact cctgttgtga tagacattgt tccaccattt attgtaatat
     3361 ttgtatttac tgatggatca aaaacttcaa aatttgctac aacatctgca ctatcgtaag
     3421 atgcaacaag tcctttgtcg gcactagtaa tattaatatc gccatcgtat attttaatat
     3481 atccttccgt acaatctaaa ccatcatcca gcgctgtaat attcaaagat ccacctttca
     3541 taacaaattt ttgtttagat tttaatccat cctttaccgc attggaaaca acaatattcc
     3601 catcatatat gcttacataa tcatcactgg caataccatg tttagccaaa ctagatacat
     3661 ttagagaacc tttcccgcta aaaatcaact caccttccga aaaaatagca gctttctgat
     3721 cttctgaata ttccgtttca acaggatata cggcagagtc ggttatataa ttctgagtac
     3781 catccgctat aaccacaaaa gttcttttgc ctgtttgaat atttatagca gaaccattag
     3841 atgatcctaa attaattcca tttagtgtca actgcgactt atgggcacta tacaatttga
     3901 acgaaccggt tcgatacata ctacttttaa gcacataatt aatgccttct aattcggatc
     3961 taacaacaat attcgttccg ttttgctcaa ctgtaattcc ttctgttgga gttggaaaaa
     4021 cagtcacaga agaagattta taagtaaacg tatatgtggt tccaaactca ttattattaa
     4081 tatcgtcctc gtcaaaatat ggtcctaagt atattttatt taattctgat acattaaatg
     4141 acgcaacatt ctcaccatca tgcaaagaaa taatataatt atcatctatt tgaggatcgt
     4201 caaaagttat actatcaatg tctgttgtat tataaatagt ctcagacaaa tctgacttta
     4261 caacatacaa gttcctcgca gaaaatgcag gtaaagaaat agttatgatc attataaata
     4321 ataattttct atacataata ataaaaataa agcactactt aaaccttaca aaaaaaaaaa
     4381 taagcacaca tacggcaaat atacgaatta attttcacat cttaattatt tttagctatt
     4441 ttgttggtta taaatttcat ttttacataa ctttgcggct tcattttata atcatgataa
     4501 caacaaaaac catagctgat ttactacatg aaatttcgtc aagaagcaat accaaagtag
     4561 gatttgttcc taccatggga gccctacatc aaggacatat ttctttagta aaaaagtgtg
     4621 taaaagaaaa tgacatttgc gtagtaagca tatttgttaa tcccacacaa tttaatgata
     4681 aaaatgacct ggctaaatat ccacgtaccc ctgatgctga cacagtttta cttgaagaag
     4741 ccggttgcga tatcttattt atgccatctg tatcagagat gtatcctacg gaggatacaa
     4801 gaaaatttga ttttggcaac attgaaaagg tcatggaagg taaattccgc cctggacatt
     4861 ttaatggagt ggcacaaatt gtaagcaagt tgttttatgc agtaaagcca aacaaagcat
     4921 attttggcga aaaagatttt caacaagttg caattattaa agctatg
//
LOCUS       DAOPID010000100         4951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_22754_length_4951_cov_4.666871, whole genome shotgun sequence.
ACCESSION   DAOPID010000100 DAOPID010000000
VERSION     DAOPID010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22754_length_4951_cov_4.666871"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..721
                     /locus_tag="PK641_04405"
                     /pseudo
     CDS             1..721
                     /locus_tag="PK641_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="lysine--tRNA ligase"
     gene            764..1777
                     /locus_tag="PK641_04410"
     CDS             764..1777
                     /locus_tag="PK641_04410"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HPG55623.1"
                     /translation="MEEKFTNKDRKVCIMGGGSWATAIAKIAMTNGRHINWYMRRQEQ
                     IDQFKASGFNPSYLSSVRFDVKKIKFYSNINLAIRYSDILIFATPSPFLKQHLVKLTK
                     SLSDKIIISAIKGIVPDENMIVSDYFQKYHDVKEENIAVIAGPCHAEEVALERLSYLT
                     VASKNLKLAEMFATRIANSYLKTSTTTDVRGIEYASVLKNVYAIASGICHGLKYGDNF
                     QAILVSNSIREMGRFVDAISKDDERNIQDAAYLGDLLVTSYSKFSRNRTFGSMIGKGY
                     SVRSSQFEMEMIAEGYFGTKCIKEINEEYKVDMPILDACYNILYERIAPNIEIKVLTE
                     KIR"
     gene            1797..3137
                     /locus_tag="PK641_04415"
     CDS             1797..3137
                     /locus_tag="PK641_04415"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HPG55624.1"
                     /translation="MEDIKLSIDSCLKFISKDNIAGYETQVKACAETLHNGTGKGNDF
                     LGWLNLPSSVTAEFLADIKATAAYLRANCDVVVVAGIGGSYLGAKAVIDSLSNSFEWL
                     QDKKPIIVYAGNNIGEDYLAELTEFLSDKKFGIINISKSGTTTETALAFRLLKTQLEK
                     QMGKAEAKKRIVAITDKAKGALRTLSTKEGYKTYVIPDNVGGRFSVLTPVGLLPIAVA
                     GLDVDSLIAGAQRMEKVCGLNTPFAENPAAVYAATRNELYRNGKKIEILVNFQPKLHY
                     FAEWWKQLYGESEGKDNKGIFNAAVDFTTDLHSMGQWIQEGERSIFETVLSIKKPNKT
                     MMVPSDEDNLDGLNFLAGKHVDEVNKMAELGTMLAHVDGGVPNLKVEIPELNAYYIGQ
                     LIYFFEKACGISGYLLEVNPFNQPGVEAYKKNMFALLNKPGYEAESKAIQARLK"
     gene            complement(3201..4844)
                     /locus_tag="PK641_04420"
     CDS             complement(3201..4844)
                     /locus_tag="PK641_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010799833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPG55625.1"
                     /translation="MITADALTVEFGGTTLFKDISFAINDGDRIALMGKNGAGKSTLL
                     KILAGEKEPTKGKMSIPKDCIVAYLPQHLMTEDGRTVFEEAAQAFASIHSMEARINAI
                     NEELATRTDYDSEEYMHLIEEVSNLGEKFYSIEEINYDGEIEKTLLGLGFKRSELHRQ
                     TSDFSGGWRMRIELAKLLLRKPDVLLLDEPTNHLDIESIQWLEDFLIENGKAVIVISH
                     DKAFVDHITTRTIEVTLGRIYDYKASYSHYLELRRERRQQQQKQFDDQQKMIAETKEF
                     IERFKGTYSKTLQVQSRVKMLEKLELIEVDEEDTSALRLKFPPAPRSGNYPVIAENLS
                     KSYGEKNVFSKAGFTIKRGEKIAFVGKNGEGKSTLVKCIMGEIPYDGTLTIGHNVQIA
                     YFAQNQASLLDSELTVFQTIDDVAVGDIRTKIRDLLGAFMFNKEESEKKVKVLSGGER
                     TRLAMVKLLLEPVNLLILDEPTNHLDLRTKDILKQALSDFDGTLIVVSHDRDFLDGLV
                     EKVYEFGGGRIVEHQEGIYDFLKRKKLENLNELERKTKK"
BASE COUNT         1570 a          801 c          943 g         1637 t
ORIGIN      
        1 cttccgtaac gagggtatgg atcgaacaca taatcctgaa tttacatgta tggaaattta
       61 tgtttcatac aaagattata actggatgat ggattttaca gagcgcatgc ttgagaagat
      121 atgtcacgat gtaaatggaa ctgacgaagt aaaagtcggt gatagtgtaa ttagttttaa
      181 ggctcctttt aaacgtatta ccatgatcga ttcaataaag gaattcacag gcattgatat
      241 tacgggaatg gatgagagtc aacttcgtga tgtatgtaag caattacata tagaaattga
      301 cgaaacaatg ggtaaaggta agattattga cgaaatattc ggagataaat gcgagggcaa
      361 ttatatacaa ccaacattta tcacagatta tcctattgaa atgtctccat tgtgtaagcg
      421 tcatcgtaat aatccagagc ttacagaaag atttgagttg atggttaacg ggaaagaatt
      481 atgtaatgca tattcagaat taaatgatcc tattgatcaa ttaggtagat tccaagatca
      541 attaagatta agtgaaaaag gagacgatga agcaatgttt attgacatgg actttgttcg
      601 tgctttggag tatggtatgc ctccaacttc aggaatgggt attggtatag atagattagt
      661 tatgcttatg accggtcaaa caactattca agaggtacta cttttcccac aaatgaaacc
      721 ggaaaagaaa caatcgtcaa atttagaaga ggagaaatag actatggaag aaaaatttac
      781 gaataaagat cgtaaagttt gtattatggg tggtggtagc tgggctactg caattgctaa
      841 aatagcgatg acaaatggtc gtcatattaa ttggtatatg cgtcgtcagg aacaaatcga
      901 tcagtttaaa gcttccggat ttaatccatc atatttgagt agtgttcggt ttgatgtaaa
      961 gaaaatcaag ttttattcaa atataaattt agctataaga tattctgata tattgatatt
     1021 cgctacgcca tcaccatttc taaaacaaca tttagtaaaa ctgaccaagt cattgtctga
     1081 caaaataata atatcagcta ttaaaggtat tgtgccggat gaaaatatga tagtttctga
     1141 ttattttcag aaatatcatg atgtaaagga agaaaatatt gctgttattg ccggtccttg
     1201 ccacgctgaa gaagttgctc ttgaaagatt gtcatatctt acagttgcaa gtaaaaatct
     1261 gaaattagca gaaatgtttg ctacacgaat tgcgaacagt tacttaaaga catctaccac
     1321 tactgatgtt cgtggtattg aatatgcatc agttttgaaa aatgtatatg ctattgcatc
     1381 aggtatttgc cacggattaa aatatggtga taattttcaa gctattttag tttcaaatag
     1441 tattcgcgag atgggtcgtt tcgttgatgc tatttcgaaa gatgatgaac gtaatattca
     1501 agacgctgca tatcttggcg acttattagt aacttcatat tctaaattca gccgtaatag
     1561 gacttttggt tctatgattg gaaaaggtta ttctgtacgt tcatctcaat tcgaaatgga
     1621 gatgattgct gaaggctatt ttggcactaa gtgtattaag gaaattaatg aagaatataa
     1681 ggttgatatg cctatattgg atgcttgtta caatattttg tacgaaagaa ttgctccaaa
     1741 catagaaatc aaagtattaa cagagaaaat tagataaact ataattactt acaaaaatgg
     1801 aagatattaa attatcaatt gacagttgtc ttaaatttat atcaaaagac aacatagccg
     1861 gctacgaaac tcaggttaaa gcatgtgcag aaacattgca taacggaacc ggtaagggaa
     1921 atgacttttt aggctggctt aatttgcctt cttctgtaac tgctgagttt ttagcagata
     1981 ttaaagcaac agcagcatat ttgcgtgcaa attgtgatgt tgtagttgta gcaggtatcg
     2041 gtggtagcta tttaggagca aaggctgtaa ttgattcatt atcaaatagt tttgaatggt
     2101 tacaagataa gaaacctatt attgtatatg ccggtaataa tattggtgaa gattaccttg
     2161 ctgaattaac ggaattcttg tctgataaaa agttcggtat cattaatatc tctaaatcag
     2221 gaacaacaac cgaaactgct ttagctttcc gtttacttaa aactcaattg gaaaaacaaa
     2281 tgggtaaagc agaagcaaaa aaacgtatag ttgcaataac agataaagct aaaggtgcat
     2341 tacgtacttt atcaacaaaa gaaggttaca aaacatatgt tattcctgat aatgttggtg
     2401 gtcgtttttc tgtattaaca ccggttggtt tgttgcctat tgccgtagca ggattagatg
     2461 ttgacagctt gattgcagga gctcaacgta tggaaaaagt ttgtggattg aatactccat
     2521 ttgcagaaaa ccctgcagct gtatacgcag ctactcgcaa cgagttatat agaaatggca
     2581 aaaaaattga aatcttagta aacttccaac ctaaacttca ttatttcgct gaatggtgga
     2641 aacaattata tggtgaaagt gaaggaaaag ataacaaagg tatttttaat gctgctgttg
     2701 attttacaac agatttgcac tcaatgggac aatggattca agaaggcgaa cgtagcattt
     2761 tcgaaaccgt tttgtctatt aaaaagccaa ataaaactat gatggttcct tctgatgaag
     2821 ataatcttga cggattaaat ttcttagcag gtaaacatgt tgatgaagtt aataaaatgg
     2881 ctgaattagg tacaatgtta gcacacgttg atggcggtgt tcctaatctt aaagtagaaa
     2941 tacctgagtt gaacgcatat tatattggtc aattaatata cttctttgaa aaagcttgtg
     3001 gaattagtgg atatctatta gaggttaatc catttaatca accgggagta gaggcatata
     3061 agaaaaatat gttcgcatta ttaaacaaac cgggttatga ggctgaatca aaagctattc
     3121 aagctcgttt gaaatagtat ttttattaat gataaaagtc cggatagaat tctacccgga
     3181 ctttttgttt ttgttatagt ctatttttta gtttttcgtt ccaactcgtt caggttttca
     3241 agttttttgc gtttcagaaa gtcgtagatg ccttcttgat gttcaactat acgtccacct
     3301 ccaaattcat atactttttc aaccaaacca tctaaaaagt cgcggtcgtg agatacaacg
     3361 attagtgttc cgtcgaaatc cgataatgct tgctttaata tatctttggt tctcaaatca
     3421 aggtgattag tcggctcatc taatattaat aaattgaccg gttcaagtaa taattttacc
     3481 atagcaagtc ttgtacgctc gccccctgat agaaccttta ctttcttttc cgattcttct
     3541 ttattgaaca taaatgcacc tagcaaatct cttatttttg tacggatatc acctacggct
     3601 acatcgtcaa ttgtttgaaa tacagttagt tcactatcca acaaggatgc ctgattttgt
     3661 gcaaaatatg ctatttgtac attgtgacct atagttaatg ttccatcgta tgggatttca
     3721 cccatgatgc acttaactaa tgtagattta ccttctccgt ttttacctac aaaagcaatc
     3781 ttttcacctc ttttaattgt gaaaccggct tttgaaaaca catttttttc gccgtaactt
     3841 ttagacaagt tttctgctat tacaggataa ttacctgaac gtggtgcagg tggaaatttt
     3901 agtcgtagtg cagatgtgtc ttcttcatca acttcaatca gttcaagctt ttccaacatc
     3961 ttaaccctcg actgtacttg tagggttttt gaataagtac ctttaaaacg ctcaataaac
     4021 tcttttgttt cagcaatcat tttttgctgg tcatcaaatt gtttttgctg ctgttggcgt
     4081 ctttctcttc ttaattccaa atagtgtgaa tacgaagctt tataatcgta gatacgtcct
     4141 aaggtaactt ctatggttcg tgtcgtgatg tgatcaacaa aagctttgtc gtgcgaaatt
     4201 actataacag ctttgccatt ttcaatcaga aaatcttcaa gccactgaat agattcaatg
     4261 tccaaatggt tggtaggctc atcaagtaat aaaacgtcgg gtttgcgcaa taataatttt
     4321 gccaattcta ttctcattcg ccatccaccg ctaaaatcac ttgtttgtct gtgcaattca
     4381 ctgcgcttaa aacctaatcc aagcaatgtt ttttcaattt cgccgtcgta gttaatttct
     4441 tctatgctgt agaatttttc gccaaggttt gatacttctt caattaaatg catgtattct
     4501 tcgctatcgt agtcagttct agttgctaac tcttcattaa tagcgttgat tcttgcttcc
     4561 attgagtgta tagatgcaaa ggcttgagct gcctcttcaa acacggtacg cccatcctcg
     4621 gtcattaaat gctgtggcaa atatgctaca atgcagtctt taggtattga catcttacct
     4681 ttggtgggtt ctttttctcc tgcaagtatc tttagtagtg tagatttgcc ggctccgttt
     4741 ttgcccatta aggcaattct gtcgccatcg ttaatggcaa aagagatgtc tttaaatagg
     4801 gtggttccac caaattctac agtaagtgca tcagcagtaa tcatataaat ttatttgttt
     4861 gttgggcata aaaaaagctg ctaaaaactt agcagctttc tgtgggcgtt gacggattcg
     4921 aaccgccgac cctctgcttg taaggcagat g
//
LOCUS       DAOPID010000101         4929 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_22904_length_4929_cov_4.624333, whole genome shotgun sequence.
ACCESSION   DAOPID010000101 DAOPID010000000
VERSION     DAOPID010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22904_length_4929_cov_4.624333"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PK641_04425"
     CDS             <1..254
                     /locus_tag="PK641_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HPG55626.1"
                     /translation="IPFGLIYVYNDVDKDSRQPKEGDIVYIRAKKSKCQKCDNHVVAE
                     GESMYDISQHYGIKIHKLYDLNNMPYSAKPKVGQILKLH"
     gene            275..931
                     /locus_tag="PK641_04430"
     CDS             275..931
                     /locus_tag="PK641_04430"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPG55627.1"
                     /translation="MDQNKRMFYHALVVPILLVILLFLVFALEYFGGYDFHFLGVYPR
                     ELKGLIGILTMPIIHSDIKHLYANIIPIFVLTLFLYMAYRKYAYKVLIFVWIVTGMIL
                     WIIGRSSWHIGISGIIYGLVCYLFFGGLAGNNRTSIAIALIVAFLYGGIVWYMIPIGI
                     DNHISWEGHLSGAVSGIIAAVIFRTKPKGDENAEKQEEQDDVQYDWRDSDKTESELPS
                     "
     gene            1001..1954
                     /locus_tag="PK641_04435"
     CDS             1001..1954
                     /locus_tag="PK641_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55628.1"
                     /translation="MEQQDSTNKNKEEMDLIDVIKIVWHWFVLYICKPFVYLFRFGLN
                     RWWQLIIFGILGFAASYMYSEFRPIYQGFVVYQTNTGISSDFITEIDQLSKSSLDNIS
                     TKLEIPEKSIVELRSIAPHYVYSVDSLLTSHIIDYYDKYLNKNKKIVINNRFCVEIRM
                     YDKSCFDDWESGFVNYFNKNKFFSDLKKKDIANVKTRIGVLEEEIEKLDSLRQIEYFE
                     NSRKNVSITSVGTISTSPETKLLHNDILSLKSELQSNKNKLIYETDVLSVVVPMQVNQ
                     LPYNTWLKTYKVFVFVGVFIGLVLLLLWDNRKKIYNFIVIK"
     gene            complement(2006..2402)
                     /gene="ssrA"
                     /locus_tag="PK641_04440"
     tmRNA           complement(2006..2402)
                     /gene="ssrA"
                     /locus_tag="PK641_04440"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2479..4017)
                     /gene="rny"
                     /locus_tag="PK641_04445"
     CDS             complement(2479..4017)
                     /gene="rny"
                     /locus_tag="PK641_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HPG55629.1"
                     /translation="MELLEIILIIAAFVLGGGIAIGLVFFIQNLRAKSIIQTAKAEGE
                     AIKKAKLLEVKEKYLQLKNEYEKQASVRNSKLQSTEARLVQKEKQLLSQEESIVKKES
                     EIEHLKTSYEGQLEIAEKKDQELDRLHHQAIEKLESIAGLSAEEAKNNMIESLKAEAQ
                     TAALAYTNEIMEEARMNANKEAKKVIIKSIQRVASEAAIENAVTVFHIESDEIKGRII
                     GREGRNIRALEAATGVEIIVDDTPEAIVLSAFDPVRREIARLSLHQLVQDGRIHPARI
                     EEIVAKVTKQIEEEIVEIGKRTTIDMGVHGLHPELVRLIGKMKYRSSYGQNLLQHARE
                     TANLCAVMASELGLNPKKARRAGLIHDIGKVADEDPELPHALLGMKIAQRCGEKPDIC
                     NAIGAHHDEVEMQTLIAPIVQACDAISGARPGARREIVEAYIKRLNDLENIAMSYPGV
                     QKTYAIQAGRELRVIVGADKISDQETERLSEDIAKRIQDEMTYPGQVKITVIREIRAV
                     AVAK"
     gene            complement(4127..4435)
                     /locus_tag="PK641_04450"
     CDS             complement(4127..4435)
                     /locus_tag="PK641_04450"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HPG55630.1"
                     /translation="MNQLQKQFEITLKINDCTFHTIIERSEEAIYREAAKQLKLKILQ
                     YQEKYSSVAKNKVFLTSLVALEYALELIKLQNEQTNNTAKISIEKMNDKILKCLSEVK
                     "
     gene            complement(4410..4730)
                     /locus_tag="PK641_04455"
     CDS             complement(4410..4730)
                     /locus_tag="PK641_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55631.1"
                     /translation="MAEVYPNSLDELEKNIDALISLSENQKEQIERLIEENSNARNEI
                     LRSHAEIMELQKKYDSLKIAKASISLNKGDVKEAQKALSEIIEEINNCIAQLEQNEPA
                     SETI"
BASE COUNT         1509 a          824 c          823 g         1773 t
ORIGIN      
        1 gaataccatt tggtttgatt tatgtttaca atgatgtaga taaagatagt cgtcagccta
       61 aagaaggtga tatagtatat atccgtgcaa aaaaatcaaa atgtcaaaaa tgtgataatc
      121 acgtagttgc tgaaggagaa tcaatgtatg atatttctca acattacggc attaaaatac
      181 ataaattgta cgatcttaat aatatgccat attctgcaaa accaaaggta ggacaaatac
      241 ttaagttaca ttgatgccgt ttttctcgca agatatggat caaaataagc gtatgtttta
      301 tcatgcgctt gttgtgccta tattactagt tattttgtta tttttagtct ttgcgctaga
      361 atattttggt ggttacgatt tccattttct aggtgtgtat cctcgtgaat taaaagggct
      421 gattggcata ttaactatgc caattattca tagcgatatt aaacatttat acgccaatat
      481 tatacccata tttgttttga ccttattctt gtatatggca tatagaaaat atgcatataa
      541 ggtattgatt tttgtatgga tagttacggg catgatacta tggataatag gaagatcttc
      601 atggcacatt ggtatatcag gtattatata tggtttagta tgttatcttt tttttggagg
      661 cttagccggc aataatagaa cgtcaatagc aatagcatta attgtggctt ttttgtatgg
      721 cggtattgtt tggtatatga taccgatagg aattgataat catatttcgt gggaaggaca
      781 tttgtccggt gctgtgtcag gaataattgc tgcagtaata tttagaacca agcctaaggg
      841 cgatgaaaat gcagaaaagc aagaagaaca agatgatgta caatatgatt ggcgagattc
      901 ggataaaaca gaatctgaat taccttcata gtacatatat acgttcaaat cgcaaaattt
      961 gatttttttt tactactttt gcagcattaa tataaataca atggaacaac aagactctac
     1021 taataaaaat aaagaagaaa tggatttgat agatgtaatc aaaattgtgt ggcattggtt
     1081 tgtactatat atatgtaaac catttgtata tctatttaga tttggtttga atagatggtg
     1141 gcagctaatt attttcggaa ttttaggatt tgctgcttcg tacatgtata gtgaattccg
     1201 tccaatatat caaggatttg tagtatatca aactaataca ggaattagta gtgattttat
     1261 tactgaaata gatcaattat caaagtcttc tttggataat atatctacaa aattggaaat
     1321 tcctgaaaaa tcgattgtcg aactcaggag tattgcaccc cattatgtat attcggtgga
     1381 ttccttactt acttcccata taattgatta ttatgataag tatttaaaca aaaataagaa
     1441 gattgtaata aataatcgtt tttgtgttga aataaggatg tacgataaga gttgttttga
     1501 tgattgggaa tctggttttg taaattactt taataaaaat aaattctttt ctgatttaaa
     1561 gaaaaaggat atagctaatg taaaaactag aattggtgta ttagaagaag aaatagaaaa
     1621 attagatagc cttcgtcaaa tagagtattt tgaaaactca agaaaaaatg tttctattac
     1681 atcagtagga actatatcga cgtcacctga aactaaattg ttacataacg atatattgtc
     1741 tcttaaatca gaattacaat caaataaaaa taagttgata tatgaaacag atgttttatc
     1801 tgttgtagtt ccaatgcaag ttaatcaatt accatacaat acttggttga agacatataa
     1861 ggtatttgtc tttgtcggag tatttattgg gttagtatta cttctattgt gggataatcg
     1921 taaaaaaata tataacttta tcgtaattaa gtaagcaagt ttgcaagata caaaaaagag
     1981 ccgcataaaa attatgcggc tctttttgtg gagctggagg gtatcgaacc ctcgtccaaa
     2041 caaggaaata atacgctttc tacatgctta ttttggctta aattttcgag atgtaacaag
     2101 acccaaacta ccaattacat ccttatcttc tgttttttac ctgcaacgcg aagccgaagt
     2161 aggcgagttc tgatatttcc gcacctccat gttcaaaaag cctcagaaca gcggcatttg
     2221 ggagatgtct cgttttagta ccttgtacca aaataaagtt agtctactat aattcgacta
     2281 agcagcaaga gcgtagttat tttcgccagt tataattttg aaagttcaga ttatagagcc
     2341 cacttacaaa gctctgcatg cttacatatc agttctacct gctgtcaaat ccagtcaacc
     2401 ccaatatgtt ccggcaaaag tacgacaaaa aaaagtccct tacaaatttg caagagactt
     2461 tttaaatatt ttgattgctt attttgcgac agctacagct ctaatttcgc gtattacagt
     2521 aatttttact tgtccgggat atgtcatttc atcttggatt ctctttgcaa tatcttcaga
     2581 caaacgttct gtttcttgat cgctaatctt atcagcccca acaataacac gtaattcacg
     2641 tcctgcctga atagcgtatg tcttttgtac tcccggataa ctcattgcga tattctccaa
     2701 atcgttaaga cgtttaatat atgcctcaac aatttctcgt cttgctccgg gtcttgctcc
     2761 ggaaatagca tcacatgctt gtacaatagg agcaattaat gtttgcattt ctacttcgtc
     2821 atggtgagca ccaattgcat tgcaaatatc cggtttctct ccacaacgtt gtgcaatctt
     2881 cataccaagt aatgcgtgtg gtaattccgg atcttcatca gcaactttac ctatatcgtg
     2941 tattaaacca gcgcgacgtg ctttcttcgg atttaagcct aattcagatg ccattactgc
     3001 gcataaattt gcggtttcgc gtgcatgttg taaaagattc tgaccatacg aagaacgata
     3061 tttcatttta cctattaaac gaacaagctc aggatgtaaa ccatgtacac ccatatcaat
     3121 tgtagtacgt ttacctattt caacaatttc ttcttcaatc tgttttgtaa cctttgctac
     3181 aatttcttca attctggcag ggtggatacg tccgtcttga accaattgat gtaatgacaa
     3241 acgtgctatc tctctgcgga cagggtcaaa tgcagataga acaattgctt caggagtatc
     3301 atcaacaata atttcaacgc cggttgcagc ctctaatgct ctaatgtttc taccttcacg
     3361 tccaatgatg cgtcccttta tttcgtcaga ttcaatatga aatacagtaa ctgcattctc
     3421 tatagctgct tcacttgcaa ctctttgtat agattttata attacttttt tagcttcttt
     3481 gtttgcattc atgcgagctt cttccataat ctcattagtg taagccaatg ctgctgtttg
     3541 agcctctgct ttcaatgatt caatcatgtt gtttttagcc tcttctgcag ataaacctgc
     3601 gatagattct agtttttcaa tagcttgatg atgtaagcga tctaattcct gatctttctt
     3661 ttcggctatt tctaattgtc cttcgtaaga agttttaaga tgttctattt cagattcttt
     3721 ttttacaata ctttcttctt gtgacaataa ttgtttttct ttctgtacca atcttgcctc
     3781 agtagattgt agcttcgaat tgcgtacact tgcttgtttc tcgtattcgt ttttaagttg
     3841 taagtacttt tcttttactt caagtaattt ggccttctta attgcttcac cttctgcttt
     3901 tgctgtttgt atgatgcttt ttgctcgcag gttctgtata aagaacacga gtccaatcgc
     3961 aatgccgcca ccaagcacga acgctgcgat gattaaaatt atttctaata gttccatttt
     4021 attttgtata tataattaat taataaaaaa aatgcaataa ctgcccgcat ttagcggaat
     4081 caggaattgc atttaattat gaaagatatt cagtagagat agtgtattac ttcacttctg
     4141 acaagcattt taaaatctta tcgttcatct tttcgataga aatcttagcc gtattattgg
     4201 tctgctcatt ctgtaatttt atcaattcaa gagcatattc tagagcaacc agcgatgtta
     4261 aaaatacttt gtttttagct accgatgaat atttctcttg atattgcaga atctttaact
     4321 ttaattgttt tgctgcttct cgatatatgg cttcttcact tctttcaata atagtatgaa
     4381 aagtacaatc attgattttt agtgtaatct caaattgttt ctgaagctgg ttcattttgt
     4441 tcgagttgtg cgatacaatt atttatctct tcaataattt ctgacagtgc tttttgtgcc
     4501 tcttttacat ctcctttatt taaagatatg ctagctttag ctatttttag actgtcatac
     4561 ttcttttgca attccatgat ttctgcatgt gaccggagta tttcgtttct tgcattgcta
     4621 ttttcctcaa ttagtctttc aatctgttcc ttttgatttt cagacaagct gatcaaagca
     4681 tcaatatttt tctctagttc atctaatgaa tttggataca cttcggccat atacataaaa
     4741 tctttacaaa agtagcaata aaggatgaga aaaaaaaacg gcagacaata aaaaaataag
     4801 atttatataa aaaaacaaaa gccgcttgtt ttaagcggct tttatatgtt tgactggtaa
     4861 aattaaatac ctttttcttt aatacgtgct tttttaccta ctaatccgcg gaggtaatac
     4921 aggcgtgag
//
LOCUS       DAOPID010000102         4868 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_23279_length_4868_cov_4.620195, whole genome shotgun sequence.
ACCESSION   DAOPID010000102 DAOPID010000000
VERSION     DAOPID010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23279_length_4868_cov_4.620195"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PK641_04460"
     CDS             complement(<1..199)
                     /locus_tag="PK641_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HPG55632.1"
                     /translation="MKKLTVIKVGGKIVEEENTLASLLTDFSSIEGNKVLIHGGGRSA
                     TRIATLLGIESKMVDGRRITDA"
     gene            complement(211..744)
                     /locus_tag="PK641_04465"
     CDS             complement(211..744)
                     /locus_tag="PK641_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPG55633.1"
                     /translation="MRKLSVTDLNRLSAEDFRSSEKLPLIVVLDNVRSMYNVGSVFRT
                     ADAFLLESIFLCGITCTPPSVEIHKTALGAEETMAWKYYADTMDAVKDLKKEGYKVLA
                     IEQAEKSVMLQNLVLSPSEKYALVLGHEVKGVSQSVVDACDGCIEIPQYGTKHSLNVS
                     VAGGMVIWECEKQLRKQ"
     gene            846..1652
                     /locus_tag="PK641_04470"
     CDS             846..1652
                     /locus_tag="PK641_04470"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HPG55634.1"
                     /translation="MPDSTSHTNNIQVIVDSTDTITAQNDTTANLISLQDTVVADSIV
                     FIPEPIRFDGIPRTQEFFKNDIFVSLILLFAVFLSLIISSQRKARIKYLDKLLNTNNN
                     QQTLTIREYGDQLILGIFAIIGYSLLACESFFGKGNWETLGLCAGGFVLFLIAKYTLL
                     YWYMRTFFPTEQTRIVKLYETLLNLTGLIAFCGYICVVFMPHIFLPYIYGLLILIAVA
                     FCVTVIYIFFTRFFNKHGLILRFILYLCTLEILPIAVLIKALLEVNGLIN"
     gene            1668..2423
                     /locus_tag="PK641_04475"
     CDS             1668..2423
                     /locus_tag="PK641_04475"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HPG55635.1"
                     /translation="MKIRKILVSQPKPSTEKSPYYDLADKYGFKIDFRPFIKVEGLSL
                     KEFRAQRIEVTDYTAVIFTARTAIDHFFRLCEEMRLTISEDMKYFCISESVAVYLQKY
                     IQYRKRKVFFSATGKFDELIPLIKKHNTEKYLIAVSDVNKDSQINLLNANKITFTKAV
                     FYKTVSNDFGPDEAFDYDMLIFFSPEGINSLLKNFPNYQQGETLIATFGASTAQAVKD
                     AGLRLDLGAPNEQAPSMTGAIDLFFKENKVNKK"
     gene            2487..2852
                     /gene="rnpA"
                     /locus_tag="PK641_04480"
     CDS             2487..2852
                     /gene="rnpA"
                     /locus_tag="PK641_04480"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HPG55636.1"
                     /translation="MDQKLPKTERLHGETSVSQLFMKGKGFSVFPLRVVYLKRNDSLP
                     DRIVASVSKKRFKHALDRNRVKRLIRETFRKNKPCMSLDIGFIFLSDQIPSYLEMESA
                     MHKVVAKLKQTDRAVLTKE"
     gene            2849..>4868
                     /gene="uvrA"
                     /locus_tag="PK641_04485"
     CDS             2849..>4868
                     /gene="uvrA"
                     /locus_tag="PK641_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPG55637.1"
                     /translation="MNSEQSYINVYGAREHNLKNIQVKIPHGSLTVITGLSGSGKSSL
                     AFDTLYAEGQRRYIETFSAYARNFLGGGERPDVDEITGLSPVIAIEQKTTNNNPRSTV
                     GTTTEIYDYLRLLFARAGEAYSYLTGEKMVKYTEDQILSLIMSEYSGKKIYLLSPVVR
                     SRKGHYRELFETLRKKGYLNVRVDGELKEITRDMRLDRYKNHDIEVVIDRLEVQDKDV
                     TRLQRSLRLALDQGEGMTAVLDKDGKKMRYFSRRLMCPTTGISYSDAAPHDFSFNSPK
                     GCCKRCHGTGYISQIDIEKLIPNRSLSIHDGGIAPLGRYKNSIIFAQIEALCEDYGAT
                     LKTAIKSLPDELIEKIIYGTEDNIIIKSLVEGLNGYPVAYNGIAEYMAMQQDSEASAG
                     AQKWAEQFSQTITCPECGGTRLKKEALYYRIADKNIAELTAMDIDELYQWLIDNEPKL
                     SSNQHKIATEIIKEIKTRLSFLINVGLHYLSLARASNTLSGGESQRIRLATQIGSQLV
                     NVLYILDEPSIGLHPRDNIKLIDSLKKLRDLGNTVIVVEHDKDMMLAADYIIDMGPKA
                     GRLGGQVVFEGTPKDMLKTDTLTTAYLTGKKAISIPGKRRQGNGDILRLIGAKGNNLK
                     NVNVEFPLGKFICVTGVSGSGKSSLIGGTLLPIISQKLYKFVYVAGLER"
BASE COUNT         1669 a          885 c          856 g         1458 t
ORIGIN      
        1 cggcatcagt aattcgcctt ccgtcaacca tcttactttc aatacccaaa agagtagcaa
       61 tacgtgttgc agaacgtcca ccgccatgta ttaaaacttt attcccttca attgaagaaa
      121 aatcagtcaa caatgatgcc aatgtatttt cttcctctac tattttcccg ccaactttta
      181 ttacagtaag ttttttcatg tctttaaact ttattgtttc cttaattgtt tctcacactc
      241 ccatattacc attcctcccg ccaccgatac attaagcgaa tgctttgtcc catattgcgg
      301 aatttctata caaccatcac aagcatctac tacactctgt gaaactccct tcacttcatg
      361 tcctaatacc aaagcatatt tttctgatgg cgaaagcact agattctgca acataacgct
      421 tttctctgct tgttcaattg ctagcacctt atacccctct ttctttaaat cttttacggc
      481 atccattgta tctgcataat atttccacgc catagtttct tcagcaccta aggcagtttt
      541 atgaatctcg acactaggag gtgtacaagt aataccacat aaaaatatag attctaatag
      601 aaaggcatca gccgttcgaa acacagaacc cacattgtac atacttcgga cattatctag
      661 aactacaatc aaaggaagtt tttcactgct gcgaaaatcc tctgccgata gacgatttaa
      721 atcggtcaca cttaattttc tcattttcat cggatgaatg acaaaaataa tagttttttt
      781 tctaattttg tcatcaatta aaaaagaaac ggaaaagcac aatcttttca ccaagtagca
      841 catctatgcc ggattccaca tcacacacaa acaacataca agttatcgtt gatagtacag
      901 atacaatcac ggcacaaaac gataccacag caaacctaat aagtttacaa gatacagttg
      961 ttgcagattc catagttttt attcctgagc ctattcgatt tgatggtatc ccaaggacac
     1021 aggagttttt taaaaacgat atatttgtca gtttaattct actctttgct gttttcctaa
     1081 gtttaataat ttcatcacaa cgcaaagcta gaatcaaata tcttgataaa ttgctaaata
     1141 ccaataacaa ccaacaaact cttactataa gagaatatgg cgatcaactt atattaggta
     1201 tttttgcaat aataggatac tcattacttg catgcgaatc tttttttgga aaaggcaatt
     1261 gggaaactct tggcttatgt gcaggaggat ttgttttgtt tttaattgct aaatacacgc
     1321 ttctctattg gtacatgcgc acattttttc cgacagagca aactcgtatt gtaaaactat
     1381 acgaaacact actaaattta acaggattaa tcgcattttg tgggtatatt tgcgttgtgt
     1441 ttatgccgca tatttttctt ccatatatat acggattact aatattaatt gctgtagcat
     1501 tctgtgttac agtgatttac atatttttta ctcgattttt caacaaacat ggcttgattt
     1561 taaggtttat tttgtacctt tgcacgctcg aaattttacc tattgcagtg ctcataaaag
     1621 cattgttgga ggttaatgga ttaataaact aaaaatcgcg tatagcagtg aaaatcagaa
     1681 aaatactcgt ttcgcaacca aagccgtcaa cagaaaaatc tccgtattac gatttagcgg
     1741 ataaatatgg ttttaaaatt gactttcgcc cttttataaa agtagaagga ttaagtttaa
     1801 aggaatttcg tgctcaacgc atagaagtta cagattatac agctgtaatt tttactgcac
     1861 gcacagctat tgatcatttt ttccgtcttt gcgaagaaat gcgtttaacc atttctgaag
     1921 acatgaaata tttctgtatt tcagaaagtg tagctgttta tttacaaaaa tacatccagt
     1981 accgcaaacg caaggtgttt ttttctgcta caggcaaatt tgatgaatta attcccctca
     2041 tcaagaaaca taatactgaa aagtatttaa tcgctgtatc agatgtgaac aaagacagcc
     2101 agataaattt acttaacgcc aacaaaataa catttacaaa agctgtcttc tataaaacgg
     2161 taagtaatga tttcggccct gatgaagcat ttgattacga tatgcttata ttttttagtc
     2221 ctgaaggaat taattcatta ctaaaaaact ttcctaatta ccaacaaggc gagactttaa
     2281 tagcaacatt tggagctagc acagcacaag cagttaaaga tgccggatta agattagact
     2341 taggtgcacc aaatgaacaa gcaccatcaa tgactggtgc tattgacctg tttttcaagg
     2401 aaaacaaagt gaataagaaa taaaaatacc ttataattat caattaatat cccaaaagtc
     2461 agtacttttg ggatatttta tttaaaatgg atcagaagct accaaaaaca gaacgactgc
     2521 acggagagac atctgtaagt caattattta tgaaaggaaa gggtttttct gtttttcctc
     2581 taagggtagt gtacttaaaa agaaacgatt ctctaccgga tagaatagtt gcaagcgtct
     2641 caaaaaaacg atttaagcat gctttagata ggaacagagt taagcgatta ataagagaaa
     2701 catttagaaa aaacaaacct tgcatgtctc ttgatatcgg ttttattttt ctatcggatc
     2761 aaatacccag ctatcttgaa atggagtcag caatgcataa agtagtagca aaattaaagc
     2821 aaacagatag ggctgtacta acaaaagaat gaattcagag caaagctaca taaatgtata
     2881 tggtgcaaga gaacacaatc tgaagaatat acaggtaaaa attccacatg gcagccttac
     2941 ggtaattaca ggtttaagtg gaagcggaaa atcttcatta gcatttgata cactatatgc
     3001 agaaggacaa agacgttata tagaaacttt ctccgcatac gccagaaact ttttaggcgg
     3061 aggcgaaagg cctgatgttg atgaaataac agggctgagt ccggttatcg caatagagca
     3121 aaaaactacc aataacaatc cgcgatctac agtaggaact actactgaga tatatgatta
     3181 cctacgtttg cttttcgcac gagcaggcga ggcatattcg tatctgacag gcgaaaagat
     3241 ggtcaaatac acagaagacc aaatcttgag ccttattatg tcagaatatt caggcaaaaa
     3301 aatatattta ttatcccctg tagttagatc acgcaaaggt cattatcgcg aacttttcga
     3361 aaccttgcgt aaaaaaggat acttgaatgt acgggttgac ggagaactaa aagagattac
     3421 gcgtgatatg cgattggaca gatacaaaaa tcatgacatt gaagttgtca tagatcgttt
     3481 ggaagttcaa gataaggatg taacaagatt acaacgctca ctccgattag cacttgatca
     3541 aggtgaaggc atgactgcag tactcgacaa ggacggcaaa aagatgcgct attttagtcg
     3601 cagacttatg tgccctacta ccggcatatc ttattctgac gcggctccac acgatttctc
     3661 cttcaattct cctaaaggat gttgtaagcg ttgccatggg accggatata tatctcaaat
     3721 agatattgag aaattaatac ctaatcgttc tttatctatt catgatggag gtattgctcc
     3781 tcttggtaga tataaaaaca gcataatatt tgcacagata gaagcacttt gtgaagacta
     3841 tggtgcaact ttaaaaactg caataaaaag cctacctgac gagttaatag aaaaaataat
     3901 atacggaacc gaagataata taattatcaa aagtttagtt gaaggtttaa atggctaccc
     3961 tgttgcttat aatggaattg cagaatatat ggcgatgcaa caagatagtg aggcatcagc
     4021 cggagctcaa aagtgggcag aacaattttc tcaaacaatt acttgccctg aatgtggcgg
     4081 cactcgccta aaaaaagaag ccctgtatta tcgtatcgca gataaaaata ttgccgaact
     4141 aactgcaatg gatattgatg aattatatca gtggttaatc gataacgaac ctaaattaag
     4201 tagtaatcag cacaaaatag ctaccgaaat tataaaagaa attaagacac gactttcttt
     4261 tttgattaat gtcggattac actatttatc tttagcacgt gcctcaaaca ctctttcagg
     4321 aggagaaagc caacgtattc gattagcaac acaaataggt tctcaattag taaacgtttt
     4381 gtatatactt gacgagccga gtataggact tcatccgcgc gacaatataa agttgattga
     4441 ttctttaaaa aaattgcggg atttaggcaa tactgtgata gttgttgagc acgacaaaga
     4501 tatgatgctt gctgccgatt atattattga tatgggacct aaagcaggtc gattaggtgg
     4561 acaagttgtg tttgaaggaa caccaaaaga tatgctaaaa acagatacgt taactactgc
     4621 ctatttaacg ggcaagaaag ctattagtat ccccggaaaa cgaagacaag ggaatggtga
     4681 tattctaaga ttaataggtg caaaaggcaa taaccttaaa aatgtaaatg tagaatttcc
     4741 attaggaaaa tttatatgtg taacaggagt ttcgggcagt ggcaaatcta gtttaattgg
     4801 aggaacacta ctgcctatta tcagtcagaa actttataag tttgtgtatg ttgccgggtt
     4861 agaacgag
//
LOCUS       DAOPID010000103         4858 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_23353_length_4858_cov_3.199667, whole genome shotgun sequence.
ACCESSION   DAOPID010000103 DAOPID010000000
VERSION     DAOPID010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23353_length_4858_cov_3.199667"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2250)
                     /locus_tag="PK641_04490"
     CDS             complement(<1..2250)
                     /locus_tag="PK641_04490"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPG55638.1"
                     /translation="MKPFLQQIAELFLSTSEYNISECCFVFPNRRAGLFFQKYLTENA
                     GRTLLAPKIQTIADFFASYSTLKQEDKFGLLFHLYDAYTKVLPDGIVPEPFDDFIYFG
                     EIILSDFNDVDNYMVNAEQLYTNIKDLKNLDNLDYLTTEQKKVLKSLLKFSDDKKNRA
                     KFIETWNWMLPLYNKFREILFEQNLAYGGMLHREVVKKERLDNEYNKVIFVGFNALNE
                     AEKTLFKLLGAKADFYWDYKSAPILDQANMAHYFMEENISKFPSKLELPKETLAEPTI
                     KCIGIPSGVGQTKEVGKILSKMDSVSINTAVVLSDEQLLLPTIYSIPDCIENVNITMG
                     YPMNHTPIQQLLEECLLLQKNLQNNTFYFKNVLAVLNHPYILRNSSQISNTINNIVKE
                     NKIRIDITELLKDNIEIYNLLFKKTDDVLSYLQDIIDYLKNKDDADEWSHEFIIQASI
                     NLKRIGILLNKWHLDLQIETILHIIRQIFGKISISFKGEPLGGLQIMGMLETRCLDFD
                     NLIITSFNEGIFPKTETANSFIPYNLRRAFNLPTTEHQDAIFAYHFYRLIQRASSVCL
                     IYDTRSESLGSTGEESRYIKQLKHLYNVNIQEHIIQYKVQINKPQDIIIKKDQKIQDI
                     LKKYLRTDDKPKTKEEEKNNYLSFSASSINTYLKCPLAFYYSHILNIKEQDEISEIME
                     SNQFGQIFHKAMELIYANNINREVDKATLDSWINDEVKLRQIVQNSFNMCYFNGKNTV
                     TFSNQFTVLE"
     gene            2351..4324
                     /locus_tag="PK641_04495"
     CDS             2351..4324
                     /locus_tag="PK641_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPG55639.1"
                     /translation="MISIDGLTVEFSERALFDNVSYVVNRRDRIALVGKNGAGKSTML
                     KIIFGIQEPTRGRISYPKDLTIGYLPQHMIHNDDTTVIEEASKAFSHIAQLQEKIERK
                     NQELCERTDYESEEYQDLIDEISSLNEHLTVLGGGNFQAEIEKTLLGLGFLRSDFDRP
                     TKEFSGGWRMRIELAKIILQCPDVLLLDEPTNHLDIESIQWFENFLASSPNAVILVSH
                     DRAFLDAVTKRTIEISLGKIYDYKANYSKYVELRKERREQQLRAYNNQQKEIQDTEDF
                     IERFRYKATKSVQVQSRIKQLERIERIEVDEEDNSSLRLKFPPAPRSGSYPVIMENVA
                     KRYGEHTVFEHVDMTINRGEKVAFVGKNGEGKSTLVKCIMGDITDFEGKLQLGHNVKI
                     GYFAQNQASLLDESLTVFQTIDYVAVGDIRTKIRDILGAFMFGGEASDKKVKVLSGGE
                     RSRLAMIRLLLEPVNLLILDEPTNHLDMRSKDVLKQAIKEFDGTAIIVSHDREFLDGL
                     VTMVYEFGGGAIRPHIGGIYEFLYAKKMETLRELERKNPVKPSVAKVPEKATKNLSSS
                     EEKSKVQADHIENELTYEQRKERNKLIKRAERLIELCEINISETETEIKHIETQMAEG
                     NADAATFEHYAELKKQLEQQVADWEKAMEDMPK"
     gene            complement(4409..>4858)
                     /locus_tag="PK641_04500"
     CDS             complement(4409..>4858)
                     /locus_tag="PK641_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HPG55640.1"
                     /translation="PYEIFTGLADDEDGLMLPKSVTTGKIIRNYHEETGVKTYDFQFT
                     NKYGYKITIEGLSHKFNPEYWNYAKLISGVLRYGMPIDQIVRLVGTLQLNNESINNWK
                     MGVERALKKYIPDGADTGQKCPHCGEKLTFTEGCMKCNNCGYSKCGG"
BASE COUNT         1634 a          741 c          814 g         1669 t
ORIGIN      
        1 ttctagtact gtaaattggt ttgagaaagt tacagtattt tttccattaa aatagcacat
       61 attaaaacta ttttgaacta tttgtctaag ttttacttca tcattaatcc atgagtccaa
      121 tgtggcttta tctacttcgc gattaatatt attggcataa attagttcca ttgctttatg
      181 aaatatttgc ccaaattgat tagattccat aatctcggaa atctcatctt gttctttaat
      241 gtttagtatg tgcgaataat aaaaagcaag tggacatttt aaataagtat ttatactgct
      301 cgccgaaaat gaaaggtaat tatttttttc ttcttctttt gtttttggtt tatcgtctgt
      361 acgtaaatat ttttttaata tgtcttgtat cttttgatct tttttgataa ttatatcttg
      421 tggtttatta atctgtacct tatattggat tatatgttct tgaatattaa cattatacag
      481 atgttttaat tgtttgatat aacggctttc ttctccggtt gaacctaaac tttcagaccg
      541 tgtatcatat attaagcata cagatgatgc tcgttgtatt aaacgataaa agtgatatgc
      601 aaaaattgca tcttgatgtt cggttgtagg taaattaaat gctcgtctta aattgtatgg
      661 aataaaagag ttagcagttt ctgttttagg aaatatacct tcattgaacg aagtaataat
      721 taggttgtca aaatccaaac atcttgtttc caacataccc attatttgta agcctccaag
      781 cggctctcct ttaaacgata tacttatttt tccaaaaatt tggcgtataa tgtgtaaaat
      841 ggtttctatt tgtaaatcta gatgccattt attcaacaat atacctatgc gtttaagatt
      901 tattgaagct tgtataataa attcgtgact ccattcatca gcatcatctt tatttttcag
      961 ataatcaatt atatcttgta gataagataa tacatcatca gtctttttaa acaacaagtt
     1021 ataaatttct atattatctt ttaataattc agttatatca attcttattt tgttctcttt
     1081 tacaatatta ttaatagtat tgcttatctg tgagctattg cgcaaaatat aaggatgatt
     1141 taaaactgcc aacacatttt taaaatagaa tgtgttattt tgcaagttct tttgtagtag
     1201 taaacactct tccaataatt gttgaatagg ggtatggttc atcggatatc ccattgttat
     1261 attgacattc tcaatgcaat ccggaattga gtatatagta ggtaacaata gttgttcatc
     1321 tgatagtaca acggctgtat ttatagatac agagtccatt ttagacaata ttttacctac
     1381 ttcttttgtt tgtcctacac cagatggtat gccaatacat tttattgtag gttcagctaa
     1441 ggtttcttta ggtaattcaa gctttgatgg aaactttgaa atgttttctt ccataaaata
     1501 gtgagccata tttgcttgat ctaatattgg agctgattta taatcccaat aaaagtctgc
     1561 ttttgcacct aatagtttaa ataaagtttt ttctgcttca ttcaaggcat taaaacctac
     1621 aaatattacc ttattatatt cattatcaag tctttctttt tttactacct ctcgatgtaa
     1681 cataccaccg tatgctaaat tttgctcaaa caaaatttct ctaaatttat tatataaagg
     1741 caacatccaa ttccatgtct caataaattt agctctgttt tttttgtcgt cactaaattt
     1801 taataaactc ttaagaacct ttttttgttc ggttgtcaaa taatccaggt tatccaaatt
     1861 ctttaagtct ttaatgttgg tatataactg ctcagcatta accatatagt tatctacgtc
     1921 attaaaatca cttaaaatta tttctccaaa ataaatgaaa tcatcaaatg gttcgggaac
     1981 tataccgtct ggtaatacct tagtatatgc atcatacaaa tggaatagta agccaaattt
     2041 atcttcttgt ttaagtgtac tatatgatgc aaaaaagtct gcaattgtct gtatcttagg
     2101 tgctaacaaa gtccttccag cattttcagt caggtatttc tgaaagaaaa gaccggcacg
     2161 tctattcgga aatacaaaac aacattctga aatattatat tctgatgtac ttaggaataa
     2221 ttctgcgatt tgctgtagaa aaggtttcat ttattctgta taattatttt tttagtcatt
     2281 agcacaaaaa tactttaaaa aatcaattaa ataatcattt aattgtaatt ttccttttta
     2341 cctttgcgcc atgatttcaa tagatggatt aacagtagaa tttagcgaac gtgcattatt
     2401 tgataatgtc agttatgtag tcaataggcg cgatcgtatt gcattggtag gaaaaaatgg
     2461 tgcaggcaag agtacgatgc tcaaaataat ttttggcata caagaaccta cgcgaggcag
     2521 aatctcatat cctaaagacc taactattgg atatttacct caacacatga ttcacaatga
     2581 cgatacaaca gttattgaag aagcatctaa agcatttagt catatagccc agttacaaga
     2641 aaaaatagaa cgcaaaaacc aagaattgtg tgagagaaca gactatgaat cagaagaata
     2701 tcaagattta atagatgaaa taagcagctt aaacgaacat ttaaccgtat taggtggtgg
     2761 aaattttcag gctgaaatag aaaaaacttt attgggatta ggatttttgc gctctgattt
     2821 tgatcgtccc acgaaagagt ttagcggtgg atggcgtatg cgtattgaat tggcaaaaat
     2881 aatattacaa tgtcctgatg ttctactact tgatgagcct actaaccacc ttgacataga
     2941 atcaattcaa tggtttgaga actttttggc atcaagtcct aatgcagtaa tacttgtttc
     3001 tcacgataga gcatttttag atgcagttac caaaagaaca attgaaatca gtttaggtaa
     3061 gatatacgat tacaaagcga attattctaa atatgttgaa ttgcgcaaag aacgccgtga
     3121 acaacagttg cgtgcatata ataatcaaca aaaagaaatt caagatacag aagattttat
     3181 agaaagattt agatacaagg ctaccaaatc agtacaagta cagtcgcgta ttaaacaatt
     3241 ggaacgaata gaaagaattg aggttgatga agaagataac agttctttaa gactaaaatt
     3301 tccaccggca ccgcgttcgg gatcatatcc tgttattatg gaaaatgttg ccaaaaggta
     3361 tggagaacat actgtatttg aacatgttga catgactata aataggggtg agaaagttgc
     3421 tttcgttggt aaaaatggtg aaggtaagag taccttggtt aaatgtatca tgggtgatat
     3481 tactgatttt gaaggtaaac tacaattagg acataatgta aaaatagggt actttgctca
     3541 gaatcaggct tctttgcttg acgaaagttt aacagtgttt caaactattg actatgttgc
     3601 cgttggtgat ataagaacaa aaattagaga tatattgggc gcatttatgt ttggcggaga
     3661 ggcttcagac aaaaaggtaa aagttctttc gggtggtgag aggagtaggt tggcaatgat
     3721 tcgtttatta ttagaacctg taaacctgtt aatcttggat gaaccgacta atcacctaga
     3781 tatgcgttcg aaagacgttc taaaacaagc aattaaagaa tttgatggta cagccattat
     3841 tgtttcacac gatcgtgagt ttttagatgg tttggtaact atggtttatg aatttggagg
     3901 tggtgctata cgtccgcata tagggggtat atacgagttt ttatatgcaa aaaaaatgga
     3961 aactttgcgc gaacttgaac gcaaaaatcc tgtaaagcca agtgtcgcca aagttcctga
     4021 aaaagcaact aaaaatttat cgtcatctga agaaaaatct aaagttcaag cagatcatat
     4081 tgaaaatgaa cttacatacg aacagcgtaa agaacgtaac aagctgatta aaagggcaga
     4141 acgattaatt gaattatgtg aaatcaacat ttcagaaaca gaaacagaaa ttaaacatat
     4201 tgaaactcaa atggcagaag gtaatgctga tgcagcaaca tttgaacatt atgcagaact
     4261 taaaaaacag ttagaacaac aagtagctga ttgggaaaag gcaatggaag atatgcctaa
     4321 ataatattca cttcattata aaaaaaatag ggcggtttct taaagattcc gcccttattt
     4381 ttcatagtat ttaatttata acatcaagtt atccaccaca ttttgagtat ccacagttgt
     4441 tacatttcat gcaaccttct gtaaatgtga gcttttcacc acaatgagga cacttttggc
     4501 cggtatcagc accatcagga atgtatttct tcaatgcacg ttcaacaccc attttccagt
     4561 tattaataga ctcattatta agttgaagag tacctacaag tctaacaatt tgatcgatag
     4621 gcatgccata gcgaagtacg cctgatatta attttgcata attccaatat tcaggattga
     4681 atttgtgtga caagccttcg atggtaatct tgtaaccata tttattcgta aattggaaat
     4741 cgtaggtctt aacacctgtt tcttcgtgat agttacgaat aatcttacct gtagttacag
     4801 actttggtaa cattaagcca tcttcatcat cagctaaacc ggtaaagatt tcgtatgg
//
LOCUS       DAOPID010000104         4840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_23490_length_4840_cov_3.894462, whole genome shotgun sequence.
ACCESSION   DAOPID010000104 DAOPID010000000
VERSION     DAOPID010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23490_length_4840_cov_3.894462"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2173)
                     /gene="purL"
                     /locus_tag="PK641_04505"
                     /gene_synonym="purI"
     CDS             complement(<1..2173)
                     /gene="purL"
                     /locus_tag="PK641_04505"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HPG55641.1"
                     /translation="MVFFFKTKSNSWYAVQTLAPLSIENTNKLVWLFDEAEALTDTEL
                     LGHFVGPRREMITPWSTNAVEITQNMGIEGILRIEEFYKETDTTEFDPMLQRKYDKID
                     QNIFTVDKQADPIIYIDDIRSYNKQEGLALNDDEITYLEGIAAKLGRKLSDSEVFGFS
                     QVNSEHCRHKIFNGQYIIDGKEKELSLFKLIKKTSEYNPNKLVSAYKDNVAFNAGPRI
                     QQFAPKSGDKPDFFQITEFDSVISLKAETHNFPTTVEPFNGAATGTGGEIRDRLGGGK
                     ASLPIAGTAVYMTSYPRSEVRSWEESIKSRNWLYQTPEQILIKASNGASDFGNKFGQP
                     LICGSVLTFEHEENDKIYGFDKVIMLAGGVGFSSMRDALKGKPEVGEKVVVMGGDNYR
                     IGMGGGAVSSVDTGQYANAIELNAVQRANPEMQKRVSNVIRTLAESEHNPIVSIHDHG
                     AGGHLNCLSELVESTGGKIDMDSLPVGDPTLSDKEIIGNESQERMGMLIKEQDIDLVD
                     KIAKRERAPMYVVGETTGDMNFVFEKKNGNKPIDLRLEYMIGNPPRTVIKDSTTKDSF
                     TSPKTDAKQINTYIEQVLQMESVACKDWLTNKVDRSITGKVARQQCAGELQLPLNDLG
                     VVALDYTGTSGIATSIGHAPMAGLADPEAGSVLSIAEALTNIVWAPLKDGLDSVSLSA
                     NWMWPCKNQGEDARLYKAVEACSDFACALGINIPTGKDSLSM"
     gene            complement(2306..>4840)
                     /locus_tag="PK641_04510"
     CDS             complement(2306..>4840)
                     /locus_tag="PK641_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55642.1"
                     /translation="NNNYWRDLAKINDIGDIHYILSTLDSLKESSPEINSFMENRKLM
                     ISTYVDSSANFKHLISTQISASEWTTVQNFMHTYVKKTFYFCYENEIFIASSNRELLD
                     ASKQQIKSKIAIIKADSAFTQISASSGTKATANWFLDMKQSKKLINRFFQSDITQILS
                     ITKDYDSWCCFDLDLSEDKIIINGFADGNGKNDIIHIFQGQTTNSNTLSSVMPYNTYF
                     FRHYAISDFGLFQNKLAEYREALSKTAHFAEESAKWETTSGESPLLFFQEYFGGEIAL
                     ACNPTNTYIIAKLYNQAGAQDRLTRMVEETEGASSNKKGTLTIFHFPLNGFAGNIFGE
                     YFNLDNEYISVVGDNLIIAPTEDLLSYIASRNKNTQTLQCSPEYKRANKTLLSTSNRT
                     IYVDIPYIIRNSEKFFVPEIADKIKSTRSLWSNFESLGFQSEAESNGNDYQHIFVQYD
                     KKSEVEIQEENKQESQSNENIAQNENSNISVAATQPVDENTSKFSKLISIELDKPARI
                     KPQFVINHYTGETEIAIQDEKNQFYLINSEGKILWKIQLPEAIIGDITQVDMLKNKKL
                     QMAFTTLNKLYIIDRTGKNLKSYPKNLPSRATAGLSVFDYDNKRDYRFFIPTENKQIN
                     LIKADGTTPQDWKFKESSGKIKQAIKFYRVNGKDYLVASDAKQCYFLDRRGRNRLIPK
                     KNIIPSANNLIYIDDFSSQKRFVMSSPDGTIVYLHQDGNVLSIKTTKRDKNHAFVVCK
                     SKQKTYYAFYDTQGFTLYDTELESIIEDNTVNSGKSPKCETYNNYIGIYDSDQEKIFV
                     YNSDIPNQKIAIASDNDLFSICQYKPHEQTCIAYCNENKLLLAKIE"
BASE COUNT         1340 a          854 c          851 g         1795 t
ORIGIN      
        1 tcatagaaag gctgtctttt ccggtaggga tattaatgcc taaagcgcaa gcaaaatcgc
       61 tacatgcttc aactgccttg tatagacgag catcttctcc ttggttttta catggccaca
      121 tccagttagc agaaagagat acgctatcca atccgtcttt aagtggagcc catactatat
      181 tggttaaagc ttctgcaata gaaagtacag aacctgcttc ggggtcagcc aagccggcca
      241 taggtgcgtg accaatagat gttgcaatcc ctgatgttcc ggtatagtca agcgctacta
      301 cacccaaatc atttagaggt aattgtaatt cgcctgcaca ttgctgacgt gcaacttttc
      361 ctgtaataga acgatctacc ttgttggtta accaatcctt acatgcaaca gattccattt
      421 gtagaacttg ctcaatgtag gtatttattt gttttgcatc tgttttaggt gatgtaaatg
      481 aatctttggt agtgctgtct tttataactg tgcgaggagg atttcctatc atatattcca
      541 aacgtagatc gataggttta tttccgtttt tcttttcaaa tacgaaattc atatctcctg
      601 ttgtttcacc aacaacgtac ataggagcgc gctcacgttt ggcaatttta tctactaaat
      661 caatatcttg ttctttgatt aacataccca tacgctcttg actctcgttg ccaatgattt
      721 ccttgtcaga tagagtaggg tctccaaccg gtaaactatc catgtcgatt tttcctccgg
      781 tagattcaac taattcagac aagcaattta agtgtccacc tgcaccatgg tcatgtattg
      841 agacgattgg attatgttcg ctttcggcta atgtacgtat aacgtttgat acacgttttt
      901 gcatttcagg gtttgcacgt tgaactgcat ttaattcaat tgcattagcg tattgacctg
      961 tatctactga tgatacagca ccaccaccca taccgattct atagttatcg ccacccataa
     1021 ccacaacttt ttcgccaact tccggtttgc ctttcaatgc atctcgcata gagctaaaac
     1081 ctacaccgcc ggcaagcatg attactttat caaaaccata tattttatcg ttctcttcgt
     1141 gttcgaatgt caatacagaa ccacagataa gtggttggcc aaacttatta ccgaagtctg
     1201 atgctccgtt tgatgcctta atcaagattt gttcgggggt ttgatataac caattacgag
     1261 atttgatact ttcttcccaa ctgcgaactt cacttcgagg gtaggatgtc atgtatactg
     1321 ctgttcctgc aattggtaag ctggctttac ctccgccaag tctatctctg atttctccac
     1381 ctgtaccggt tgctgcacca ttaaaaggtt caacagttgt cgggaaatta tgtgtttctg
     1441 ctttaagtga gattactgaa tcgaattcgg ttatttggaa gaaatcaggt ttatctccac
     1501 tcttaggtgc aaattgttga atacgaggac ctgcattaaa tgctacattg tctttatatg
     1561 cagaaaccaa tttgttagga ttgtattctg atgttttttt gatgagtttg aaaagagata
     1621 attctttttc ttttccatca attatgtatt ggccattgaa tattttgtga cggcagtgct
     1681 ctgaattaac ttgtgaaaat ccgaatactt cgctatcgct taatttgcgt cctaattttg
     1741 cagcgatacc ttcaagatat gtaatttcat catcattaag ggctaagcct tcttgtttgt
     1801 tgtatgaacg aatgtcatca atataaataa taggatctgc ttgtttgtca accgtaaata
     1861 tgttttgatc aattttatca tatttacgtt gcaacatagg atcgaattct gttgtatcag
     1921 tttctttata aaattcttca atacgtaaga tgccttcgat acccatgttt tgtgttattt
     1981 caacagcatt agtactccat ggggttatca tttcgcgacg cgggccaacg aagtgtccaa
     2041 gcaattccgt atcagtcaaa gcttctgctt catcgaaaag ccacactaat ttgttcgtgt
     2101 tctcaatact taatggtgct aatgtttgca ctgcatacca actattgctt tttgttttaa
     2161 agaaaaatac catttttatt gtaaatatgt tttaaggtgc aaatttactc aatttttaat
     2221 acatttctgc ttgatatata ttaaagttat caactgatgt taaaatacca ctgataataa
     2281 gttttaaatt taatcgatta ctttattact ctattttagc aagtaataat ttgttctcat
     2341 tgcaataagc tatgcaggtt tgctcgtgtg gtttatattg acaaatggaa aatagatcat
     2401 tgtctgatgc aattgcaatc ttttgatttg gaatatcaga gttgtataca aatatttttt
     2461 cttgatctga atcgtaaatt cctatataat tattgtatgt ttcacacttt ggcgatttgc
     2521 ctgaatttac agtgttatct tctataatgc tctctaattc agtatcatac aaagtaaaac
     2581 cctgagtgtc ataaaatgcg tagtatgttt tttgtttaga tttacatact acaaatgcgt
     2641 ggttcttatc tctttttgtt gttttaatag ataacacatt tccgtcttga tgtaaatata
     2701 caattgtacc atcgggacta ctcattacaa atcttttttg agatgaaaaa tcatctatgt
     2761 atataagatt gtttgctgat ggtatgatgt ttttcttagg tataagtcga ttacgtcctc
     2821 ttctatccag aaaatagcat tgctttgcat ctgatgcgac caaataatcc ttaccattta
     2881 cacgataaaa tttgatagct tgtttgattt tacctgacga ttctttaaat ttccaatcct
     2941 gaggggttgt tccatcagct tttataagat taatttgttt attctcagta ggaataaaaa
     3001 atcgataatc gcgtttatta tcataatcaa aaacagataa tcctgctgtt gctcttgatg
     3061 gtaaattttt tggatacgat ttcaaatttt tgccggttct gtctattata tatagtttat
     3121 tgagtgtggt aaatgccatt tgtagttttt tgtttttaag catatctact tgtgtgatat
     3181 caccaataat agcttcaggc aattgtatct tccaaagaat ttttccttcg gaatttatta
     3241 aatagaactg atttttttcg tcttgtatgg ctatttcagt ttcgcctgta taatgattaa
     3301 taacgaattg aggtttaatt cgcgcaggtt tatccaattc tatagaaatt agttttgaga
     3361 atttacttgt attctcatcc accggctgtg ttgctgcaac agaaatattt gaattttcgt
     3421 tttgtgcaat gttttcgtta ctttgtgact cttgtttatt ttcttcttga atttcaactt
     3481 ccgatttttt atcgtactga acaaatatgt gctgatagtc atttccgtta ctctctgctt
     3541 ctgattgaaa accaagactt tcaaagttac tccataagct gcgtgtagat tttattttgt
     3601 ctgcgatttc aggaacgaaa aatttttcag aatttcggat tatatatggg atatcaacat
     3661 aaatagttct atttgaggtg gataatagag tcttattggc tcgtttatat tcgggcgaac
     3721 attgaagtgt ttgagtgttt ttgttgcgtg atgcgatgta gcttaataga tcttcggtag
     3781 gtgctataat taaattatcc ccgactacag atatgtattc attatcgaga ttaaaatatt
     3841 caccaaaaat attaccggca aatccattaa gcgggaaatg aaaaatagtt aatgttcctt
     3901 ttttgtttga tgaggcgcct tcggtttctt caaccattcg agtcagtcga tcttgtgcac
     3961 ctgcttggtt gtatagtttt gcaataatat aagtgtttgt tggattgcaa gctaatgcaa
     4021 tttctcctcc aaaatactct tgaaaaaata ataacggact ttcgccggaa gtagtttccc
     4081 attttgcaga ttcttctgca aaatgcgctg tttttgatag ggcttctcta tactcggcaa
     4141 gcttgttttg gaatagtccg aagtcactta tggcgtaatg tctgaagaaa tatgtattgt
     4201 atggcatcac agaagacaaa gtattgctat ttgttgtttg cccttggaat atgtgtatga
     4261 tatcgttttt gccattccca tcagcaaagc cgttaataat gattttatct tcggatagat
     4321 ccaaatcaaa gcaacaccag ctatcatagt ctttggtgat gctaagaatt tgcgtgatgt
     4381 cagattgaaa gaaacgatta atcagttttt tactctgctt catgtctaaa aaccaatttg
     4441 ctgtggcttt tgtccctgaa ctagcgctta tttgagtaaa agcggaatct gctttaatta
     4501 tcgcgatttt tgatttaatt tgttgctttg aggcgtctaa taactctcta tttgatgagg
     4561 ctataaagat ttcgttttcg taacaaaaat agaatgtttt ttttacgtaa gtgtgcatga
     4621 aattttgtac ggtagtccac tcacttgcag atatctgagt acttataaga tgtttgaaat
     4681 ttgcagaact atcaacataa gtgcttatca tcagtttcct gttttccatg aatgaattaa
     4741 tttcaggaga actttcttta agtgagtcta aggtgctaag tatgtagtga atatcaccaa
     4801 tatcgtttat tttagctaaa tctctccaat agttattatt
//
LOCUS       DAOPID010000105         4832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_23548_length_4832_cov_4.094620, whole genome shotgun sequence.
ACCESSION   DAOPID010000105 DAOPID010000000
VERSION     DAOPID010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23548_length_4832_cov_4.094620"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..154
                     /locus_tag="PK641_04515"
     CDS             32..154
                     /locus_tag="PK641_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55643.1"
                     /translation="MQEVDCVIDGGGGSLEPSTIIDCTGDKPYLKRKGKGEVIL"
     gene            complement(166..1758)
                     /locus_tag="PK641_04520"
     CDS             complement(166..1758)
                     /locus_tag="PK641_04520"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPG55644.1"
                     /translation="MKYKIFACLASLLFFFNSVDAQTPSMPERPRIVVGITIDGLRSD
                     YLSLFWNSLSKGGFKRLAEQGAVFPNVRYSYQSADASVDVASFATGAEPYIHGIVGDS
                     KFDRKLSRQVKSASDASVIGLSDTRTLSAKNLQCTSLADELQEASINGSKIVSVGIDP
                     TKAILQLGHVGNCFWMNNISGNWASSNYYMTSLPEWVQNINNKKTIDTYLSKTWETMY
                     PAGYYVSASTNGGSGFKYEVRAACNGLHLYDNFATTPFANDYLCDFALEAIEQLKMGK
                     DMIPDLMLLQFSAQPFFLKAEFGLSMELEDAYLRLDKNLQWLFENLDNKFGKDNVLIY
                     LTTTRTDDAMKTTRMNPNVPGKSFNVDRYLALLNSYLMANYGQFKWVLSCGRGNIYLN
                     RELIEEKGLNLKDVQNKAVEFFYLIPGVVNVATSFDLEQAYFASGSLRYAYYRESSGD
                     LLYTLSPGWYEVDINNRPNGYKSNSSQTVPLYFYGWRIAPQLVSDQVDAADAVFTLSR
                     WLRIPAPNGSKGVVLPVKLNDK"
     gene            complement(1820..2311)
                     /locus_tag="PK641_04525"
     CDS             complement(1820..2311)
                     /locus_tag="PK641_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55645.1"
                     /translation="MLLCGCTVNCDDVPEITKQYFPYTKNQVLQYVSNENEQMELVVD
                     SIKEFHAGCFSVSAMCACESSIRWFIIDNNGESFYIPELIYTYGKGGLVALVLSQQIA
                     GGEDYRSLYFDKPDFNTADDLFGDTIKCATLGKYKDFTVVFNKGLVSFTDTITYQTWN
                     LVE"
     gene            complement(2962..3537)
                     /gene="pnuC"
                     /locus_tag="PK641_04530"
     CDS             complement(2962..3537)
                     /gene="pnuC"
                     /locus_tag="PK641_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HPG55646.1"
                     /translation="MDLKLILQIIGLILGLLYLWLEYKANIWLWLIGIIMPIVHGILY
                     LTSGLYADAAMNVYYVVAGLYGWFMWSRKPNGSDAAVIKIGHTPLKKIVPLVLAYAIA
                     HVLIYYVLVNFTDSTVPFCDSLTTALSIVALWMLSRKYIEQWLVWLVVDAITVGLYLY
                     KEIPITAGLYTLYTCMALAGYFRWCRMVSEC"
     gene            complement(3542..>4832)
                     /locus_tag="PK641_04535"
     CDS             complement(3542..>4832)
                     /locus_tag="PK641_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPG55647.1"
                     /translation="HYTYGNGYYEQYKNNRSLYTYMLDNLIDKDDNPLSKSNLVRKKG
                     MYNHFGGFVSSANYQISKLKLSFGLAWNTYAGDHYGDVLNVVDAVNFNAPFEYYRNSS
                     IKHDGNVFVKANWEIFKGFHLYADVQYRFVNQRIWGKDDLYDYNTSDMQEININKIFN
                     FINPKLGINYNFLKYNSVYASFAMANREPTRKDFVNAEKGKDPLSEQLYDIEFGYRLN
                     HKYVELGLNFYYMIYKDQLVLTGEQNPDTYEALYMNVPNSYRRGIELSIMGHPLKWFS
                     LGGNLTLSQNRVLDYTEYVSNSDTWEMDAIYIGTSTLSYSPSVIASTDFNFHTHGFEV
                     DIFTQYVGKQYITNGQNENLTIDDYCVTNLNLAYTLKTKKIESVRFGIALNNIFNSKY
                     CANAFAYSEISGGERYDEGYYLPQAQFNFLANVTIKF"
BASE COUNT         1646 a          917 c          694 g         1575 t
ORIGIN      
        1 agtaccaatc cggaattgat tgaagaaaat tatgcaggaa gtcgattgcg taattgatgg
       61 cggtggaggt tctttagagc cttcaacaat aattgactgc acaggagaca agccttacct
      121 taaacgtaaa ggtaagggcg aagttatact ctagtaaatt aatttttact tatcattcag
      181 cttcacaggt aacacaactc cttttgaacc atttggtgcc ggaatacgta accatctgga
      241 taatgtaaat actgcatcag ctgcatccac ttgatctgat accagctgtg gcgcgattct
      301 ccatccgtag aaatatagtg gtactgtttg gctgctattt gacttatatc cattgggtct
      361 gttattaatg tcaacctcgt accagccggg cgagagagta tataacaaat cgcctgaaga
      421 ttcgcgataa tatgcatatc gcaatgaacc tgaagcaaaa tatgcttgtt ctaaatcaaa
      481 ggaagttgcc acattaacca cacccggtat caaataaaag aactcaacag ccttattttg
      541 cacatctttt aaattcaatc ctttttcttc tatcagttcc ctattaagat atatatttcc
      601 tcttccacaa gataaaaccc atttgaattg tccgtagttt gccattaaat acgaatttaa
      661 cagagccaaa tatctatcaa cattaaacga tttacccggc acattaggat tcattcgagt
      721 agttttcatt gcatcatcag ttcgtgtcgt tgtaagataa atcaagacat tatccttccc
      781 gaatttatta tctaaatttt cgaacagcca ttgtaaattc ttatccaaac gcaaataagc
      841 atcttctaat tccatgctta aaccaaattc cgcctttaag aaaaatggtt gagccgaaaa
      901 ttgaagcaac atcaagtcgg gaatcatatc tttgcccatt tttaattgct ctattgcttc
      961 taaagcaaaa tcacataaat agtcatttgc gaatggtgtt gtagcaaaat tatcatacaa
     1021 atgcaagcca ttacaagctg cacgtacttc atacttaaag ccgcttccac catttgttga
     1081 tgcagataca taataacctg caggatacat agtttcccat gtttttgaca ggtatgtatc
     1141 tatggttttc ttgttattga tattctgaac ccactcgggc aagctggtca tgtaataatt
     1201 agatgaggcc caattccctg atatattatt catccaaaag caatttccga catgtcctaa
     1261 ttgcaatatt gctttagtag gatctatacc caccgaaaca atttttgagc cgtttataga
     1321 tgcttcttgt aattcatcag ctaagcttgt acattgtaaa ttttttgcag ataaagttct
     1381 tgtatcagat aagcctatta ccgaggcatc agaagccgac tttacttgtc tgcttaattt
     1441 cctgtcaaac tttgaatctc ctactattcc atgtatgtat ggttcagcac ctgtagcaaa
     1501 acttgcaaca tcaaccgaag catcagccga ttgatatgaa tatcttacat ttggaaaaac
     1561 agcaccttgc tctgccaaac gcttaaagcc acctttcgac aagctattcc aaaataaaga
     1621 aaggtaatca gaacgtaaac catctattgt gattcccaca actattctag ggcgttccgg
     1681 cattgatggt gtttgagcat caacagagtt gaagaaaaac aacagtgacg ctaggcaagc
     1741 aaaaatttta tacttcatac aatcgtgtta aaagaatgta caaagttagt aaaaaactta
     1801 attatgcgat taagtatact tattccacta aattccaagt ttgatacgtg attgtatcgg
     1861 taaaagacac caaacccttg ttaaatacta ctgtaaaatc tttatacttt cctaaggtag
     1921 cgcacttaat ggtatctcca aataaatcat ccgccgtgtt aaaatcaggc ttatcaaaat
     1981 ataaagaacg atagtcttca ccacctgcaa tctgctggga taataccaaa gcaaccaagc
     2041 ctccttttcc ataagtataa atcaattcag gtatgtaaaa agattcgcca ttattatcga
     2101 tgataaacca ccgtatagaa gattcacaag cacacattgc agatacacta aaacaacctg
     2161 catgaaattc cttaatggaa tccactacta attccatttg ctcattttcg ttactaacat
     2221 attgtaatac ttgatttttc gtataaggga agtattgttt tgttatttca ggtacatcat
     2281 cacaattcac tgtgcatccg cacaataaca ttgtgcatgc tataattaaa aatttaactt
     2341 tttttcataa ttataatttt cttttgatta tcctataaag gtaatacaat aatttttaat
     2401 aaaaatgact ttattctgtt ttttaatatt aaaaattcaa ataagaagtg cgaaacgcaa
     2461 aagaaatggc aaaatagttg aatgccaata aacatatgtc aaaagttgga attatttaat
     2521 gcaataaaag ttatttcact tttaatacgt agattttttg tattgttacg cctgcagatg
     2581 ttgcgtttct cggaattacg aaagacagag ttttaatagg taattgggac ttacctatat
     2641 cttcgatgta ttcacaacct gaaaaacgat ataagatcga agttgttctg ttttgtgtat
     2701 tgtaatcggt tgttgttcag catataactt tcgtaagttt gcgcatacaa agcacaattt
     2761 atacacgcta ataacatttt aatgattctc attttcttat tttaattttt gtactgaatg
     2821 gagttttatc tataacaata tatacaatat agattacagc agcaaaatac gaaacggata
     2881 tttgtgattc agtataattg cgcttataaa cttaatattt gttttacaaa aaacaactat
     2941 tatttttaaa acaatcaaat cttaacattc gctcaccatt ctacaccatc tgaaatatcc
     3001 cgctaaagcc atacaagtgt agagtgtata taaacctgca gttatgggta tttctttgta
     3061 taaatataat ccgactgtaa tagcatccac aaccaaccat accaaccatt gctcaatata
     3121 tttacgcgat aacatccaca aagccactat actaagagca gtagttaaac tatcgcagaa
     3181 cggcacggtg ctatcagtaa aattaaccaa cacataatat attaaaacat gagcaattgc
     3241 ataagctaat actaaaggaa ctatcttttt tagtggcgta tgaccaattt taataaccgc
     3301 agcatcagac ccattcggct tcctactcca cataaaccaa ccatacaaac ctgcaactac
     3361 ataataaaca ttcatggcag catctgcata tagtcccgat gtaagatata agattccatg
     3421 tacaataggc ataattattc ctatcagcca aagccaaata ttggccttat actcaagcca
     3481 taaatacagt agaccaagta tcagccctat aatttgaaga attaatttta aatccatttt
     3541 attaaaattt tattgtaaca ttagccaaga aattaaattg agcctgaggc aaataatacc
     3601 cctcatcata acgttctcca ccagatattt cggaatacgc aaaagcatta gcacaatact
     3661 tcgaattaaa tatgttatta agtgcaatac caaatctcac actttcgatt ttttttgttt
     3721 taagagtata tgcaagattt aaatttgtaa cacaataatc atctattgta agattctcat
     3781 tttgaccatt ggtaatatac tgtttaccaa catattgagt aaaaatatca acctcgaaac
     3841 catgtgtatg gaaattgaaa tctgttgatg ctataacact aggtgaatac gacaaagttg
     3901 atgttccgat atatatggca tccatttccc atgtgtccga atttgataca tattctgtat
     3961 aatcaagcac tctattttga ctcaaagtta agttcccgcc taaagaaaac cattttagag
     4021 gatgccccat aatcgatagt tctataccac gacgataact gtttggaaca ttcatatata
     4081 aggcttcata agtatccgga ttttgttcgc ctgttaaaac taattgatct ttgtaaatca
     4141 tataatagaa attaagtcca agttctacat atttatgatt taaacgatag ccgaattcaa
     4201 tatcatataa ttgttctgat aatgggtcct tccctttttc tgcattcaca aaatctttac
     4261 gagtcggctc tctatttgcc atagcgaatg atgcatatac actattgtat ttcaagaaat
     4321 tatagtttat acctaattta ggatttataa aattgaaaat cttattaata ttaatttctt
     4381 gcatatctga tgtattgtaa tcatacaagt catctttgcc ccatattctc tgatttacaa
     4441 aacgatattg aacatcagca tataaatgaa aacctttgaa tatttcccaa ttagctttta
     4501 caaaaacatt accatcgtgt tttattgatg aatttctata atattcgaat ggagcattaa
     4561 agttaacagc atcaacaaca ttaagcacat cgccgtaatg gtcgcctgca taagtattcc
     4621 atgctaaacc gaatgataat ttaagcttgc tgatctgata atttgctgaa cttacaaatc
     4681 ctccaaaatg gttatacatt ccttttttac gtacaagatt acttttactt aatggattat
     4741 catccttatc gattaaatta tccaacatat aagtatacaa actcctgtta ttcttgtact
     4801 gctcgtaata accgttacca taagtataat ga
//
LOCUS       DAOPID010000106         4820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_23641_length_4820_cov_4.341238, whole genome shotgun sequence.
ACCESSION   DAOPID010000106 DAOPID010000000
VERSION     DAOPID010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23641_length_4820_cov_4.341238"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..475
                     /locus_tag="PK641_04540"
     CDS             191..475
                     /locus_tag="PK641_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55648.1"
                     /translation="MDDHTYPNDKTRGYMMQVDASATPGQFFRSQIDDLCAGASLYFS
                     VWAANSIKKTYENNYQKASLSFVIEDINGNVLLTENTGLMPGTNNDNDWN"
     gene            530..1738
                     /locus_tag="PK641_04545"
     CDS             530..1738
                     /locus_tag="PK641_04545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HPG55649.1"
                     /translation="MNNGNSSSGNDLVLDDIEIRLCVPPTNVIISPNDTICKEETVIL
                     AGQFTNDGTFTEPLTYKWFKSATASYNQSDWTEIAIGQTLTIPSPQSSDAGFYRLAVA
                     SIGSIDFVNCRSMSDIVSLTVQDCQINSLVVSADTAICLGASVELHASGAVSYSWSPA
                     IGLSSTIIPNPIASPTTTTTYTVTATFADNSSMSASVTVSILTNITTILKDSICQNDS
                     YMKNGFNLPIQTESGVQTHVLNLLSQTGCDSIVQLNLQVFPEYETTRKVFIEQGESIQ
                     IDGQTYSFPTEVISRYTSVSGCDSVIITTISLNDISSECDELIPDKYFSPNDDDIQDT
                     WNIKNIECYDGYVVEIFDRFSKLLIRYEGNFTPWDGMYLGHPEQATDYWYVITLKEGE
                     TMSGHFILKR"
     gene            complement(1828..2001)
                     /locus_tag="PK641_04550"
     CDS             complement(1828..2001)
                     /locus_tag="PK641_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008153459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone H1"
                     /protein_id="HPG55650.1"
                     /translation="MKKLAATIEEKMQFFAENAKAQVENGNKAAGMRARKASLELEKL
                     LKEFRKQSIEAAK"
     gene            complement(2126..3397)
                     /gene="hisD"
                     /locus_tag="PK641_04555"
     CDS             complement(2126..3397)
                     /gene="hisD"
                     /locus_tag="PK641_04555"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007214514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HPG55651.1"
                     /translation="MQIYNNPKSEVWAELLSRPKIDNSGLENTVSNVLKDIQNNGDKA
                     VFEYEERFDHVKLSNLEVSKAEIDAAEHLIPTELKDAINTAKSNIYTFHAAQKHDLPK
                     IETTNGVCCWQKDVAIEKVGLYIPGGTAPLFSTVLMLAVPAKIAGCKEIVLCTPPDKN
                     GNVNAAILYAAKVSGVGKIFKIGGIQAIGAMAYGTESVPKVYKIFGPGNPYVTTAKQL
                     VSLKDVAIDMPAGPSEVAIIADETAKAAVVAADMLSQAEHGSTSQAIVFTTNADLAVK
                     IAEAASKQCELSSRKEFISKSLENSRIIILPEAEIIDAVNEYAPEHLIIMCKNYIKIS
                     EQIVNAGSVFLGYYTPESAGDYASGTNHTLPTSGYAKAYSGVNLDSFIKKITFQEISK
                     EGLDNLGNTIKIMAEHEQLTAHKYAVTVRQQ"
     gene            complement(3399..4250)
                     /gene="hisG"
                     /locus_tag="PK641_04560"
     CDS             complement(3399..4250)
                     /gene="hisG"
                     /locus_tag="PK641_04560"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005789131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HPG55652.1"
                     /translation="MLRIAVQAKGRLHEETIKMLEESSIKIEVSKRLLLIQSPNFPVE
                     ILFLRDDDIPQAVATGIADVGVVGENEFVERNEDAEIIKRLNFSKCRLSLAIPKAADY
                     KGLEWFNGKKIATSYPGILDSFLKSKGINADIHVITGSVEIAPGIGLADCIFDIVSSG
                     STLVSNNLKEVEVVMYSEALMIGNKNLSSEKRAILDEMLFRFSSYKNAEDKKYIILNI
                     PNDKIDDVCNLLPGMKSPTVTPLVKEGWSSMHSVIAEETFWDIINQLKAIGAEGILVV
                     PIEKMIL"
     gene            4410..>4820
                     /locus_tag="PK641_04565"
     CDS             4410..>4820
                     /locus_tag="PK641_04565"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPG55653.1"
                     /translation="MSDLFININQVLPRIPGKVFSKPFNFQIKQGQCWTFYGKNGSGK
                     TLLSEIICGRYGLKSGVIEYPFIDEIRKTVSEYIYPRQYIKTVGFQSTYSMSDFRDAY
                     YQQRFNSQEVDWYPTVAELLSAFDDKQWLNELIDL"
BASE COUNT         1465 a          838 c          830 g         1687 t
ORIGIN      
        1 agttggtaga gcatcagctt cccaagctga gggtcgcggg ttcgaacccc gttttccgct
       61 cttgttcaaa taaaacagct ccctatttat agactacttg acgtagtcgt ctggtgggct
      121 tctttatgtc gtactaactt ttcactactc atgaaaaata tattattata cactgcatta
      181 ctggacacac atggatgatc atacttaccc taacgacaaa actcgtggtt atatgatgca
      241 agtggatgct tcggcaactc ccggacaatt tttcagaagt caaatagatg atttgtgtgc
      301 aggagcttca ttatattttt ctgtttgggc agcaaattct attaagaaga cttatgaaaa
      361 taactatcag aaagccagct tgtcgtttgt tatagaggat attaatggga atgtattatt
      421 gacagaaaat acaggattga tgccgggtac aaacaatgac aatgattgga attaatatgg
      481 agcaacattc acggtgcctt tgggacaatc atctattatt tttaaaatat tgaataatgg
      541 gaatagttca agcggcaatg acttagtatt agatgatata gaaattcgtc tttgtgtgcc
      601 tcctactaat gttattatat ctcccaatga tactatttgt aaagaagaaa cggtaatttt
      661 agcaggacaa tttactaatg atggcacttt tacagaacct ttgacatata aatggtttaa
      721 aagtgcaaca gcttcttata atcagtctga ttggactgaa atagctatag ggcaaacctt
      781 aaccattcct tctcctcaat cttcagatgc aggtttttat agattagccg tagcaagtat
      841 cggaagtatc gattttgtaa attgtcgatc aatgtcggat attgtttcgt taacagtaca
      901 agattgtcaa ataaattctt tggttgtttc ggcagatact gccatttgct tgggggcttc
      961 ggtcgaatta catgcaagtg gagctgtgag ttatagttgg tctcctgcaa taggtttaag
     1021 tagtactatt ataccaaatc ctatagcctc tccaactact acaacaactt atacggttac
     1081 agctacattt gctgataatt caagtatgag tgcttcagta accgtatcaa ttttaacaaa
     1141 cataacaact atattgaaag atagtatttg tcaaaatgat tcttatatga aaaacggttt
     1201 taatttgcca atacaaacag aatcaggagt acaaacgcat gttttaaacc ttttatcgca
     1261 aaccggttgt gacagcattg ttcaacttaa tttgcaagta tttcctgagt atgaaactac
     1321 tcgtaaggta tttattgaac aaggggaaag tatacagata gatggtcaaa catattcttt
     1381 cccaaccgaa gttataagca gatatacatc agtatcgggg tgtgacagtg ttattataac
     1441 aacaataagt ttgaatgata tttcttctga atgtgatgaa ttgattcccg ataaatattt
     1501 ttcgcccaat gatgatgata ttcaagacac ttggaacatt aaaaatatag aatgttatga
     1561 tggctatgtg gtggagatct ttgacaggtt cagcaaatta ttgattcgtt atgagggcaa
     1621 ctttactccg tgggatggaa tgtatttggg acatccggaa caggctactg actattggta
     1681 tgtgataacc ctgaaggaag gggaaacaat gtcgggacat tttatcctaa aaaggtaggc
     1741 tttttattat aaatactaat tttaagcaat cagacataaa aaaaggttgg atgatttttc
     1801 atccaacctt tttttatgct gttaatatta ttttgcagct tcaatagatt gtttacggaa
     1861 ttctttcaat aatttttcaa gttccaaaga agctttgcgt gcacgcattc cagcagcttt
     1921 gtttccgttt tcaacttgtg cttttgcatt ttctgcaaaa aattgcattt tttcttcgat
     1981 agtagcggct aactttttca taatcgcgat ttaaataatt tatgaattaa tttttataac
     2041 actctcgcaa atgtaaaggc ttttttttga tttcgcaaaa aaaaccttac taaaatgcct
     2101 ttataatgcg atttttcaaa taaatttatt gttgacgtac agtaacagca tatttatgtg
     2161 ctgttagttg ctcgtgttcg gccattatct taattgtatt gcctaaattg tccagtcctt
     2221 cttttgaaat ttcctgaaaa gttatctttt ttataaaact atctaaatta actccgctat
     2281 atgcttttgc ataaccactg gtaggtaaag tatgattggt tccggaagca taatctccgg
     2341 cactttcagg tgtataatat ccaaggaata cagatccggc atttacaatc tgttcactta
     2401 tttttatgta gtttttgcac ataataataa ggtgttcagg tgcatattca tttactgcat
     2461 caataatttc tgcttcgggc aaaataataa tacgactatt ttcaagcgat tttgaaataa
     2521 attctttacg tgatgacaat tcacattgct tgcttgctgc ctcagctatt ttgactgcta
     2581 gatctgcatt cgtagtaaat acaatagctt gactcgtact tccatgttca gcttgagata
     2641 acatatctgc agcaactaca gcagcttttg cagtttcgtc tgcaattatt gcaacttccg
     2701 atggccctgc cggcatatca attgcaacat cttttagact aactaattgt tttgctgttg
     2761 ttacataagg atttccgggt ccaaatattt tatatacttt tggaacactt tctgtgccgt
     2821 aagccatggc tccaattgct tgaataccac ctattttaaa tatttttcca actccagaaa
     2881 cctttgctgc atataaaata gctgcattta cattgccgtt tttgtcgggt ggggtacaca
     2941 aaactatttc cttgcatcct gcaatctttg caggtacagc caacattaat actgtagaaa
     3001 ataaaggagc agtacctccg ggtatatata atcctacttt ttcgatagca acatcttttt
     3061 gccagcagca gactccattt gttgtctcta ttttaggcaa atcgtgtttt tgtgcagcgt
     3121 ggaatgtata aatattagat tttgctgtat taatggcatc ctttagttct gttggaatta
     3181 agtgttcggc tgcatcaatc tcagctttac taacttctaa gttcgacagt ttaacatggt
     3241 caaagcgttc ttcatattca aaaacagcct tgtctccatt gttttgaatg tcctttaaaa
     3301 cattagaaac agtattttcc aatccagaat tgtcgatttt tggtcgagat aacaattctg
     3361 cccatacttc agatttagga ttattatata tttgcatctt aaagaatcat tttttctata
     3421 ggtactacaa gaataccctc agccccaatt gcttttagtt gattaattat gtcccagaat
     3481 gtttcttcag caattacaga atgcatagaa gaccatcctt cttttaccaa aggcgttact
     3541 gtcggtgatt tcatgccggg tagcaaattg catacatcat ctattttgtc gtttggtatg
     3601 tttagtatta tatatttttt gtcttcagca tttttgtatg atgagaatct gaatagcatc
     3661 tcatctaaaa tagcgcgttt ttcagaagac agatttttat taccaatcat aagagcttcc
     3721 gaatacataa ctacttcaac ttcttttaag ttgttgctta ccaaagtgct accggaactt
     3781 acaatatcaa aaatacaatc agccaaaccg ataccgggtg caatttcaac cgatcccgta
     3841 atcacgtgaa tatcagcgtt tattcctttg cttttcaaga atgaatcaag aatgccgggg
     3901 tatgatgttg ctatcttttt cccattaaac cattctaatc ctttataatc agcggctttt
     3961 ggtattgcaa gagataatct gcatttacta aagttaagac gtttgataat ttctgcatct
     4021 tcatttcttt ctacaaactc attttctcca actacaccaa catcagcaat acctgtagca
     4081 actgcttgag gtatatcgtc gtctcttaaa aataaaattt cgaccggaaa atttggtgat
     4141 tgtatcaata gtaatctttt gcttacttct attttgatac ttgattcttc aagcatctta
     4201 atagtctctt catgcaacct acctttggct tgtacagcaa ttcgtaacat ggcttatatt
     4261 atcgttaaat gatgatttcc gcaaaagtac aaaaaaatat ccgtatcttt acccctttaa
     4321 tgtataaagt atatatagtt ttaatattag aacttaagta ataacaagta gtaaagattt
     4381 agtactttgc tatctaaaat atatggtgga tgagtgatct ttttattaat ataaatcaag
     4441 tgcttcctcg tatcccggga aaggtttttt caaaaccgtt taattttcaa atcaaacaag
     4501 gacaatgttg gacgttttat ggaaaaaatg gaagtggaaa aaccttactt agtgaaatta
     4561 tttgcggcag atatggcttg aaatcgggtg ttattgagta cccttttatt gatgaaataa
     4621 gaaaaaccgt ttcggagtat atttatcctc gacaatatat taaaacagta ggatttcagt
     4681 caacatattc gatgtctgat tttagagatg cctattatca acaaagattt aactcgcaag
     4741 aagttgattg gtacccaaca gtggcagaat tattgtctgc ttttgatgat aagcagtggc
     4801 ttaatgaatt gattgattta
//
LOCUS       DAOPID010000107         4813 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_23686_length_4813_cov_4.079655, whole genome shotgun sequence.
ACCESSION   DAOPID010000107 DAOPID010000000
VERSION     DAOPID010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4813
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23686_length_4813_cov_4.079655"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="PK641_04570"
     CDS             complement(<1..1052)
                     /locus_tag="PK641_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPG55654.1"
                     /translation="MEYNFKEIEQKWQSYWKSNAIYHVEIDNNRPKYYVLDMFPYPSG
                     AGLHVGHPLGYIASDIFSRYKRLQGFNVLHPMGYDAYGLPAEQYAIQTGQHPEITTFN
                     NINRYRDQMDKIGFCYDWDREVRTCDPKYYKWTQWAFIQMFDHWYCKRADAARPISQL
                     IEIFNEQGNAKVKAACAETPIFTAEEWKSWDEKRQRDILMNYRIAYKADTMVNWCPKL
                     GTVLANDEVSEGLSVRGGYPVEQKVMSQWCLRVSAYAQRLLDGLDKIDWTDSLKETQR
                     NWIGRSEGAEMKFKIAGSNEYMTVFTTRADTTFGVTFMVLAPESEYVSKITTDAQKAE
                     VNEYLDAVKRRTERER"
     gene            1269..2012
                     /locus_tag="PK641_04575"
     CDS             1269..2012
                     /locus_tag="PK641_04575"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HPG55655.1"
                     /translation="MTQKDITKYVLIGVSAIILIMLSASSFYTIDTGERGVILRFGRL
                     TTIADEGLNVKVPFVDEVKKLSIRDQNLSLHTEVSSSDIQTIAVDVSLVYSLDSKRVD
                     KIFQTYGMDIENTLIRPTLSEKINAVIAEYPIETFVEKRAEISNRINTTFANQVTGSG
                     IKVKSLLITNHDFSDDFNKAIEDKKIAEQGALTAKFTLERMKLEAEAQKVKQASLSPM
                     VLQEMAINKWDGKMPQYFSGAQLPFITIK"
     gene            2032..3132
                     /locus_tag="PK641_04580"
     CDS             2032..3132
                     /locus_tag="PK641_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008666103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HPG55656.1"
                     /translation="MYLNHLSILNYKNLAEVELDFEKGLNCLIGDNGVGKTNLLDAIY
                     YLACCKSRLTPIDLQNIKHDEPFMMVQGNFDRAGVLEEIGIGLKRGQRKSFKRNGKEY
                     DRLSDHIGLVPLVIVSPEDSSLITDGSEIRRKFMDGVISQYNREYLYKLIRYNKLLLQ
                     RNTLLKSQDTEDSLYDVCELQMEELAVYLYQQRLSFIKEFVPIFNRYYSLVSQEKEKV
                     GINYRSHLQDGELKPQLEFCRRRDSILGYTSKGIHKDELELTLGDYPIKMVGSQGQGK
                     SFVISLRLAQFDFLKKANGGETPLLLLDDIFDKLDAGRVTQIIKIVSGDGFDQIFITD
                     TNREHLDKLLSDTVRNYRIYRVESGKIQYASI"
     gene            3129..3419
                     /locus_tag="PK641_04585"
     CDS             3129..3419
                     /locus_tag="PK641_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005775689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HPG55657.1"
                     /translation="MRKEHIQPIGDILKEYLKVFRLQSKIDETTLITSWKQVLGPSIN
                     SYTRDLSIRNKILYVSLTSSALRNELMLMRTRLIKELNDVVGTDVIEDIKFR"
     gene            3416..4111
                     /locus_tag="PK641_04590"
     CDS             3416..4111
                     /locus_tag="PK641_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HPG55658.1"
                     /translation="MNEIQQEIQSNIKRIIDGIPQGVRLVAVSKYNSNDRIEAAYSAG
                     QRLFGESRAQELVPKAQALPKDIRWHFIGHLQTNKVKYIVPYVSCIESVDSFRLLSEI
                     NKQAQKINRVIPCLLQFHVAIEETKFGFDLDECEQMLLSNDYKEFCNVSIIGVMGMAS
                     ITTDTDYIRKEFRHVKQIYTKLKAGFFANDDKFSEISMGMTHDYKIAIEEGSTIVRVG
                     SGIFGERMYINHS"
     gene            4243..>4813
                     /locus_tag="PK641_04595"
     CDS             4243..>4813
                     /locus_tag="PK641_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPG55659.1"
                     /translation="MDNKKRVYTFGNGKAEGKADMKNLLGGKGANLAEMNLIGVPVPP
                     GFTITTEVCNEYYEIGKEKVISFLKDEVLKAVAHIEGLMKSKFGDVENPLLVSVRSGA
                     RASMPGMMDTILNLGLNDKVVEGMVRKTGNPRFVWDSYRRFVQMYGDVVLGMKPVNKS
                     DIDPFEEIIESVKSAKGVKLDTELSVEDLQ"
BASE COUNT         1607 a          738 c          910 g         1558 t
ORIGIN      
        1 atacgttcgc gttcggtacg gcgttttact gcatcaagat attcgtttac ctctgccttt
       61 tgtgcatcag ttgtgatttt tgaaacatat tcgctttctg gagccaatac cataaaagta
      121 acaccaaatg ttgtgtcggc tcttgtggtg aaaactgtca tgtattcatt ggatccggct
      181 attttaaatt tcatttcggc accttcactt cggccaatcc aattacgctg agtttcttta
      241 agagaatcag tccaatcaat tttatccaag ccgtcgagca gacgttgagc gtaggcagat
      301 acacgcaaac accactggct cattactttt tgttctaccg gataaccacc gcgaaccgaa
      361 agcccttcgc ttacttcgtc gtttgccaaa acagttccca atttaggaca ccaattaacc
      421 attgtgtcag ccttgtatgc aatacgatag ttcatcaaaa tatcgcgttg tctcttttcg
      481 tcccaacttt tccattcttc agctgtaaat ataggagtct cagcgcaagc tgcttttact
      541 ttcgcatttc cctgctcatt aaatatttct attaattgag aaataggacg tgcagcatca
      601 gcgcgtttgc aataccagtg gtcaaacatt tggataaatg cccattgtgt ccatttatag
      661 tattttggat cgcaagtgcg gacttctctg tcccaatcgt aacaaaaacc tattttatcc
      721 atttggtctc tgtagcgatt gatattatta aaagtggtaa tttccggatg ttgtcccgtt
      781 tgaattgcat attgttctgc aggtaatcca taagcatcat atcccattgg atgcaaaaca
      841 ttaaatcctt gtaatcgctt atatctactg aatatgtctg aagcaatgta tccaagtgga
      901 tgccctacat gcaatccggc acccgatggg taagggaaca tatccaaaac ataatattta
      961 ggacgattgt tgtcgatttc aacatgataa atggcattgc ttttccaata tgattgccat
     1021 ttttgttcaa tttctttaaa attgtactcc ataatgctct gtgtagacta aatttacctt
     1081 gatttaaaca gggattttaa ttataggctg caaaggtaca ttttttttgt tgtttttatg
     1141 ccttgtttat tagatgatta aatcttgatg ctcgattttg ttttttatgc acattaaagt
     1201 atcaagtatt ttgtgtaaat ttgcccacta atattataat aattatgatt tatcaacaac
     1261 aaaacactat gacacaaaaa gacatcacta agtatgtatt aattggcgtt tcagctataa
     1321 tactaattat gttatctgcc tcaagttttt atacaattga caccggtgaa aggggcgtta
     1381 tattacgttt cggacgtctt acaacaatag ctgacgaagg tttgaatgta aaagttcctt
     1441 ttgttgatga agtgaagaaa ttgtcaatac gtgaccagaa tctatcgctt catacagaag
     1501 tttcatcaag cgacatacaa acaatcgcag ttgatgtaag tttggtatat tctttggatt
     1561 caaaacgagt tgataaaata tttcaaactt atggaatgga tatagaaaac accttgattc
     1621 gtccaacatt atcagaaaag attaatgctg taatagccga gtatccaatc gaaacattcg
     1681 ttgaaaaacg tgctgagatt tcaaatagaa tcaatactac atttgcaaat caagtaacag
     1741 gcagtggtat taaggtaaaa agtttactaa ttactaatca tgattttagt gacgatttca
     1801 acaaggcaat tgaggataaa aaaattgcag aacaaggagc acttactgct aaatttacac
     1861 ttgaacgtat gaaacttgaa gctgaagcgc aaaaagttaa acaagcatca ttatcaccaa
     1921 tggtattgca agaaatggca ataaacaaat gggatggtaa aatgcctcaa tatttttcag
     1981 gagctcaatt accatttatt acaattaaat agttgatggt aaaaagaatc catgtacctg
     2041 aatcatcttt caatattaaa ttacaagaac cttgccgagg tagaacttga cttcgaaaaa
     2101 gggcttaatt gcttgatagg cgacaatggt gtgggtaaaa cgaatttgtt ggatgccata
     2161 tattatttgg catgttgcaa gagtcgctta actcctattg atttgcaaaa tataaagcac
     2221 gatgagccat ttatgatggt tcaaggcaat tttgatagag caggtgtttt agaagaaata
     2281 ggaatcgggt taaaaagagg gcaaagaaag tcttttaaac gtaatggtaa agaatatgat
     2341 cgtttgtccg accatatagg tctggtacca ttggttattg tatcacctga ggattcatct
     2401 ctaattactg atggtagtga gatacgcaga aaatttatgg atggtgttat ttcgcaatat
     2461 aatcgcgaat atttatacaa gttaattcgt tataataaat tactgcttca acgcaataca
     2521 ttgctaaaaa gtcaagatac cgaagactct ttatatgatg tatgcgaatt acaaatggaa
     2581 gaattggcag tttatttgta tcaacaacgg cttagtttta taaaagaatt tgtgcctatt
     2641 tttaatcgat actatagttt agtatcacaa gagaaagaaa aagtaggaat aaactatcgt
     2701 tctcatttac aagacggaga attaaagccg cagttagagt tttgcaggcg tcgtgacagt
     2761 attttaggat atacttcaaa aggtattcat aaagatgaat tggagctgac tttaggcgat
     2821 tatcctatta aaatggtggg ttctcaagga caaggaaaaa gttttgttat atcccttagg
     2881 ttggctcaat ttgacttttt gaaaaaagct aacggaggtg aaactccact attattattg
     2941 gatgatattt ttgataaatt agatgccgga agagtgacac agatcattaa aatagtttca
     3001 ggagacggat ttgatcaaat atttattaca gatactaatc gtgagcattt ggataaacta
     3061 ttaagtgata ctgttcgtaa ttatagaatt tatagagttg aatccggaaa aattcaatat
     3121 gcaagcatat gagaaaagag catattcagc caataggtga catattgaaa gaatatttaa
     3181 aggtttttcg tttacaatca aaaatagatg aaacaacttt aattacatct tggaaacaag
     3241 tgctaggccc ctctattaat tcctacactc gtgatttgtc tattagaaat aaaattttgt
     3301 atgttagttt aacatcttca gctttgcgta atgaacttat gttgatgcgc actcgtttaa
     3361 taaaagagtt gaatgatgta gttggaacag atgtaattga agatataaaa tttagatgaa
     3421 cgaaattcaa caagaaattc aatcgaatat taaacgtata atagatggaa taccacaagg
     3481 agtgcgttta gttgccgtat ctaagtataa ttctaatgat agaatagagg cagcatattc
     3541 agcgggtcag cgtttatttg gagaaagccg cgcacaagaa ctagtaccta aagcacaagc
     3601 tttacccaaa gatatacgtt ggcattttat tggacattta caaacaaaca aagtgaagta
     3661 catagtacct tatgtatcat gtatagagtc agtcgattct tttcgtttat tatcggaaat
     3721 aaataaacaa gctcagaaaa taaatagggt gataccttgt ttattgcagt ttcatgtagc
     3781 tattgaagaa actaagtttg gatttgattt agatgaatgc gaacagatgt tattgtcaaa
     3841 tgactacaaa gaattttgca atgtttcaat aataggagta atgggaatgg cttctattac
     3901 aactgatact gattatatac gcaaagaatt tcgtcatgtt aagcaaattt ataccaaact
     3961 taaagctgga ttttttgcaa atgatgataa gttttcagaa atttcaatgg gtatgacgca
     4021 cgattataaa atagcaattg aagagggttc tactatagtg cgtgtaggga gtggaatttt
     4081 tggagaaaga atgtatataa atcattctta gaaataaagt tcaaacgttt gctaatattt
     4141 ttcatattag gtttcagttt ttcgataaaa ttgactaact ttgcgccctc ttatttcaaa
     4201 tacttcgtaa aatctagata atatacttta ttaaaataat ttatggataa caaaaaacgc
     4261 gtttacacct tcggaaacgg aaaagctgaa ggaaaggcag atatgaaaaa tctgcttggc
     4321 ggtaaaggtg ctaaccttgc tgaaatgaat ttaataggag tgcctgttcc tcctggtttt
     4381 acaatcacta ctgaagtttg taatgaatat tacgaaatcg gtaaggaaaa agtgatatca
     4441 tttcttaaag atgaagttct taaagcagtg gctcacattg aaggtttgat gaaatcaaaa
     4501 tttggtgacg ttgagaatcc tttattagtt tcagtacgct caggagctcg tgcatctatg
     4561 ccgggtatga tggataccat cttaaactta ggtttgaatg ataaagtagt tgaaggtatg
     4621 gtgcgcaaaa caggtaatcc tcgttttgta tgggattcat atcgtcgttt tgttcaaatg
     4681 tatggtgatg ttgttttagg tatgaaaccg gtaaataaat cagacatcga tccgtttgaa
     4741 gaaattattg aaagcgtaaa atctgcaaaa ggtgttaagt tggatacgga attaagtgta
     4801 gaagatttac aac
//
LOCUS       DAOPID010000108         4781 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_23910_length_4781_cov_3.947524, whole genome shotgun sequence.
ACCESSION   DAOPID010000108 DAOPID010000000
VERSION     DAOPID010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23910_length_4781_cov_3.947524"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..2662
                     /gene="clpB"
                     /locus_tag="PK641_04600"
     CDS             71..2662
                     /gene="clpB"
                     /locus_tag="PK641_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HPG55660.1"
                     /translation="MNFERFTIKSQEAIQKSLEMAQNAQNQAIETPHLLKALLQVGEE
                     IAKYVFAKLNVSTQNLNAELDRQIQTYPKVSGGNQYLSNETNKVLERAISLSDKKGNQ
                     FVPLEMILLALFEEKNAAGTILKNIGVNSSDLNKAIDELTKDTKTNSKSAEESYQSLS
                     KYAINLNEKARTGKLDPVIGRDEEIRRVLQILSRRTKNNPILIGEPGTGKTAIAEGLA
                     HRIVRGDVPENLKTKQIYSLDMGALIAGAKYKGEFEERLKAVINEVVKADGEIILFID
                     EIHTLVGAGGGEGAMDAANILKPALARGELRAIGATTLDEYQKYFEKDKALERRFQVV
                     MVNEPDEASTISILRGLKERYENHHKVRIKDDAIISAVELSNRYISDRFLPDKAIDLI
                     DEAAARLRLEMDSVPEELDTIDRNIRQLEIEREAIKRENDKEKVAQLTSEIENLQEEE
                     KKQKAKWQSEKNLIDKIQQNKIDIENLKFEAEKAERNGDYGKVAEIRYGKLKEKETEI
                     AEFQKKLQEMQNNNPMVKEEVDSEDIAEIVSKWTGIPVKKMVQSEQEKLLNLEAELHK
                     RVIGQDEAITAISNAVRRSRAGLKDPKRPIGSFIFMGTTGVGKTELAKALAEFLFDNE
                     NMITRIDMSEYQEKFSVTRLIGSPPGYVGYDEGGQLTEAIRRKPYSVVLFDEIEKAHP
                     DVFNVLLQVLDDGRLTDNKGRTVNFKNTIIIMTSNLGSSLIRDNFEKINSINRNDIIE
                     TTKNQVFELLKKSIKPEFLNRIDDLIMFTPLNEAEIRQIVELQVGGISKMLQDNGIMF
                     SLTSKAIDYISNEGYDPQFGARPIKRALQRLVLDELSTQIIAGTINKEKPIVINFIDD
                     KLVMKNE"
     gene            complement(2872..3687)
                     /locus_tag="PK641_04605"
     CDS             complement(2872..3687)
                     /locus_tag="PK641_04605"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPG55661.1"
                     /translation="MKAKRKYILVPFDYSDYSIKAGLLAFDLANKFNIEIIFLHAYQD
                     EESNVSEAANNAKDFYDKEIKAGRLPDAAYSFILRKGVPEDEIKRFIRRDEPLVIIMG
                     TRGKDRKINDLIGSVTAEVMDISEAPVFAVPESVELKSFNEIENIGYATSLSDPDLKA
                     FESMMEFMKPYNFTIMFVHILESGEVASLYKGKIDYLQNYISDKYPNINTAFKLIPAE
                     KSLAVELNDYYVANQIDVLAVKSNHRNIFSRIFVASLAKKLVFQAKTPLIVLH"
     gene            3795..>4781
                     /locus_tag="PK641_04610"
     CDS             3795..>4781
                     /locus_tag="PK641_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPG55662.1"
                     /translation="MQQTPFDYSIVKSVIDSFHLPDFSKATIREIVNMALQIEKQTGK
                     RFARMEMGIPGIPAPEIGVEAEIEALKNGVASTYPLLSGIPELKNESSRFIKAFIDVD
                     INPECCVPAVGSMNGAYASFLMAGQCDEKKDTILFIDPGFPVQKMQIQVLGYKIASFD
                     VYDYRGDKMKDKIESYLSHGNIAAIVYSNPNNPSWVCLTNKELKIIGELSEKYDTIVI
                     EDLAYFAMDFRKNIGTPFKAPFQASVAKYTKNYILLISGSKAFSYAGQRIGIIAMSDY
                     LYSKKYTALKKRYGVDEFGNVLISRVLYSLSAGVTHSTQYAMAAMMKAANDGK"
BASE COUNT         1699 a          736 c          922 g         1424 t
ORIGIN      
        1 gtttaatatg tggcaaagtt tttgaacatt cataattgga atcactaaag attccggaaa
       61 ggatattatt atgaactttg aaagattcac cattaaatcg caagaagcta tccaaaaatc
      121 attggaaatg gctcaaaatg cacaaaatca ggcaattgaa acccctcatt tgctcaaagc
      181 acttttgcaa gtaggcgaag aaattgcaaa atatgtattt gcaaaactta atgtaagtac
      241 acaaaatctt aatgcagaat tagaccgcca gatacaaaca tatccgaaag tatcaggtgg
      301 taatcaatat ttgagcaacg agacaaacaa ggtgcttgaa cgtgctattt ctttgtctga
      361 taagaaagga aatcagtttg tcccattgga gatgatattg ttggctttgt tcgaagaaaa
      421 gaatgctgca ggtaccatac ttaaaaatat aggtgttaat tcttctgatt tgaataaggc
      481 aatagatgaa ttgactaaag atactaaaac aaactctaaa tcggctgaag aaagttatca
      541 gtctttgtct aagtatgcaa ttaatttgaa tgaaaaagca agaacaggaa agctggatcc
      601 ggttataggt cgtgatgaag aaatcaggcg tgtattgcag attttaagta gacgaactaa
      661 aaataaccct atattaatag gagaaccggg tacaggtaaa actgctattg cagagggttt
      721 agctcaccga atagtacgtg gcgatgttcc tgaaaacttg aaaaccaagc agatatactc
      781 attggatatg ggtgctttga tagccggtgc aaaatacaaa ggagagtttg aggaacgttt
      841 aaaagcagta ataaacgaag ttgttaaagc agatggcgaa ataatcttgt ttattgatga
      901 gatacataca ttggtaggtg ccggtggtgg cgaaggcgct atggacgctg ctaatattct
      961 taaacctgca ttagctagag gtgaattacg tgcaattggt gctaccacgc ttgatgaata
     1021 tcaaaagtat tttgaaaagg ataaagcatt ggaaagaaga ttccaggttg taatggttaa
     1081 cgagccggat gaagcaagta caatctctat tttgcgtggt ttgaaagaga gatatgaaaa
     1141 ccaccacaag gttcgtatta aagatgatgc tattatttct gctgtagagt tatcaaacag
     1201 atatatatct gataggtttt tacccgataa agccattgac cttatagatg aggctgcggc
     1261 acgtttacgt cttgaaatgg actctgtgcc tgaagaattg gacacaattg accgtaatat
     1321 aagacaatta gaaattgaac gtgaggcaat taagcgtgaa aatgacaaag aaaaagtagc
     1381 tcaattgaca agtgaaatag aaaacttgca agaagaggaa aaaaaacaaa aagctaaatg
     1441 gcaatctgaa aagaatctta tcgataaaat tcagcaaaat aagattgata ttgaaaatct
     1501 taaatttgag gcagaaaagg cagaacgtaa tggagattat ggtaaagttg ctgaaataag
     1561 atatggaaaa ctaaaggaaa aagaaactga aatagccgaa tttcagaaga aactgcaaga
     1621 aatgcaaaat aataacccaa tggtaaaaga agaggttgat agtgaggata ttgcagaaat
     1681 agttagcaaa tggacaggaa tacctgtaaa gaaaatggtt caaagtgaac aagaaaaact
     1741 attaaatctt gaagcagagc tgcacaaacg tgttattggt caagacgagg ccattacagc
     1801 catttcaaat gcagtaaggc ggagtagggc gggcttaaaa gatccgaagc gccctatcgg
     1861 ttcctttata tttatgggca ccacaggtgt cggtaaaaca gaattggcta aggctttggc
     1921 tgaattcttg tttgataacg aaaatatgat taccagaatt gatatgagtg agtatcaaga
     1981 aaaattttct gtaacaaggt taataggttc acctccggga tatgtaggtt atgacgaggg
     2041 tggtcagcta actgaggcaa ttcgccgtaa accttattcg gtggtattgt ttgatgaaat
     2101 tgaaaaagct catcctgatg tattcaatgt attacttcag gtactagacg atggtagatt
     2161 aactgataat aaaggacgta ctgtcaattt taaaaacacg ataataataa tgacatctaa
     2221 tttaggttca tcattaattc gtgacaactt tgaaaagatt aacagcatta atcgtaatga
     2281 tatcattgag accaccaaaa accaagtgtt tgaactattg aaaaagagca ttaaacctga
     2341 gtttttgaat cgtattgacg atttaattat gtttacgccg cttaatgagg ctgaaatacg
     2401 tcaaattgta gaattacaag taggtggcat ctccaaaatg ttacaagaca atggaataat
     2461 gttcagctta acaagtaagg ctattgatta tattagcaat gaaggatatg atcctcaatt
     2521 cggtgctagg ccaattaaaa gagctttaca acgtttggtt ttagacgagt tatcaacaca
     2581 aattattgca ggtacaataa ataaagaaaa gccgattgta attaatttta ttgacgataa
     2641 gttggttatg aagaatgaat agtgtgtaat gtgtagaggc gaattgcatt cgcccttcaa
     2701 attcacaatg cgataaatgt ttcgaaatgc aatcgtaggg gcatgccatg gcgtgccctt
     2761 cacattacat tttcctattt aacataatat taattatggg acaaaaggaa gtaaacatat
     2821 aaatggccac ttgcagacca tttatatgtt actatattga tttgtttagg attaatgtaa
     2881 aactatcaac ggagttttag cttgaaaaac taatttcttt gctaagcttg ctacaaatat
     2941 tcttgagaaa atatttctgt gattactttt tacagccaat acatcaattt gatttgctac
     3001 atagtaatca tttaattcta ctgctaaaga tttttctgca ggaattaatt taaaagcagt
     3061 attaatgttt ggatatttgt cacttatgta gttctgtaaa taatctattt tacctttata
     3121 taaagatgct acttcccctg actccaaaat atgaacaaac ataattgtaa aattataagg
     3181 cttcataaat tccatcatgc tttcaaatgc ttttaaatca ggatcgctta aacttgtagc
     3241 gtagcctata ttttcaattt cattaaaaga ctttaattca acactctcag gaactgcaaa
     3301 aaccggagct tcgcttatat ccataacttc tgcagtaacg cttccaatta aatcgttgat
     3361 tttgcggtct ttaccacgtg ttcccataat aattaccaat ggttcatctc ttctgataaa
     3421 gcgttttatt tcatcttcag gaacaccttt acgtaatata aaagaatatg ctgcatcagg
     3481 taaacgtcct gcttttattt ctttatcata aaagtctttt gcgttattgg cagcttcaga
     3541 cacattggat tcttcatctt gatatgcgtg caaaaatatt atttcgatat tgaatttatt
     3601 agctaagtcg aatgctaata agcccgcttt tatcgagtaa tcagaataat cgaacggtac
     3661 gagaatatat tttcgttttg ctttcataag ttacaaaaat aacgttattt tgcaactctg
     3721 caaaattata ttttataact ttgcactgcc taagtaaaaa aatatgcaat ttatatagtt
     3781 caattataat tattatgcaa caaactcctt ttgattattc aatagtaaaa tctgtaatag
     3841 atagttttca tttacctgat tttagtaaag ctacaatacg cgaaattgtt aatatggcat
     3901 tgcaaataga aaaacagaca ggtaaacgct ttgccagaat ggaaatgggc ataccgggca
     3961 ttcctgctcc tgaaatagga gttgaagccg agatagaagc tctaaaaaat ggagttgctt
     4021 caacataccc cctactctca ggcattcctg aacttaagaa cgaatcatca cgttttataa
     4081 aagccttcat agatgttgat attaatcctg aatgctgtgt gcctgctgta ggttcaatga
     4141 atggtgctta tgcatctttt ctcatggcag gtcagtgcga tgaaaagaaa gatacaatat
     4201 tgtttatcga tccgggattc ccggtacaaa agatgcaaat acaggtatta gggtacaaaa
     4261 tagcatcttt cgacgtttat gattacagag gcgacaaaat gaaagacaaa atagaatctt
     4321 atttgtcgca cggaaatatt gcggctatag tatattcaaa tcccaataat ccgtcttggg
     4381 tatgccttac caacaaagag ttaaaaataa ttggagaatt gtcagaaaaa tacgacacaa
     4441 tagttataga agacttggct tattttgcaa tggattttag aaagaacatc ggaactccgt
     4501 ttaaagctcc atttcaggca tcagtagcaa aatatactaa aaattacata ttgcttattt
     4561 caggttcaaa ggcattcagc tatgccggac aacgcatcgg cataatcgct atgtctgact
     4621 atttgtattc aaaaaaatac acagctttaa agaaaagata tggggttgat gaatttggta
     4681 atgttttgat tagtagagtt ttgtatagct tgtcagccgg agtaacccac tctacccaat
     4741 acgcaatggc tgccatgatg aaagctgcca atgatggtaa a
//
LOCUS       DAOPID010000109         4780 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_23918_length_4780_cov_3.416085, whole genome shotgun sequence.
ACCESSION   DAOPID010000109 DAOPID010000000
VERSION     DAOPID010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23918_length_4780_cov_3.416085"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PK641_04615"
     CDS             <1..396
                     /locus_tag="PK641_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55663.1"
                     /translation="QSKYDGKDLWKKYDYHDFGIIGEPYFDTDFCDFFYITDTGRMWD
                     RYNVSVRDKIPVHQDRWISQGLVYHYTKDICKAIDLGTFPKRMMITTHPQRWTNNTIE
                     WMRELLLQNVKNVIKFLIKRMKKSISSLH"
     gene            546..2069
                     /locus_tag="PK641_04620"
     CDS             546..2069
                     /locus_tag="PK641_04620"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPG55664.1"
                     /translation="MKNKIFYFPNYKLILLDLVGLLLSYVIVIGITPTHAENPWAKYL
                     PIIGIYAFLWFFFGYLFRRYVGRLKNRGYLKTAFNSLYCSFAAYLISLILFLYVLNGY
                     SISVLSVFTIIAFAISFIGDTIYFAYMYALDPEEVQPISVREHKSVITKPFTLDEEAY
                     SNREHAILNLTGKKSLEYFSEFVDLRSSNTNVLSTTQLFNVEQIRPYNFDVIINLRSL
                     NNIRGINKMFCLINEKLPDNGIYIGCFKDKSVKKKQILDKYPKGINWVIYTMYYIIKR
                     LLPKLFITRHLYYDITQGKKRVLSKTEVFGRLYYCGFEVLDEKKIECYTYFIARRKQS
                     PPARKKRRYGPIIALNRVGKNGKVFKFYKMRTMYPYSEFLQDYIYQKCSLQEGGKFNH
                     DIRVNTIGRIMRKFWIDELPMMINLIKGDMKLVGVRPLSNQYFNLYCDELQKMRVKFK
                     PGLLPPFYADMPKTLDEIQASEMKYLRLCEKHGYFVTDIQYLWKIFANIVFKHARSH"
     gene            2078..3841
                     /locus_tag="PK641_04625"
     CDS             2078..3841
                     /locus_tag="PK641_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55665.1"
                     /translation="MKRYKSLIFALCCFVSALAQDIPEHISYTRVYDFVDELAIDGIV
                     EVNSVIKPYSRNFIAQKLAEASKKDSLLNKRQKSDLKFFMSEFAIELDTMPKAYVHWT
                     DKKSFDLSLLQPQFMYRDKYFKCQVRPIIGMDLLYNRHGLIMKRWYGADIQMDIVKHV
                     SVWGSIRDISFNGDYLNDTYFPDQYAKINGAKISQSGFLNDLPGCAYKESNSGGDYSD
                     FRGGIKAYVWWGSIGLVKDNIVWGDNYHGSNIISGRAPSFPMITLNLKPCKWFEFNYI
                     HGWMVSNVIDSSYYYNEEQYTDSTSKRHYRPMNKFIAANMFTFTPYPKLNISIGNSII
                     YAERSVQAIYFIPFAFFKSLDHLMTKDLRVENQNSQIFINVSSRNIKHLHLYASFFID
                     EFSINRWKPDNKERNLVSYKAGFNLSNWPVKNLAVNFEYTRSNILTYKHSIDAITWAS
                     NDYNLGHYLGDNSQEFFVSVNYKPIRSLNLNLSYTNSQKGRDYKYVRTNTKEILSQDV
                     LNEFTYKNEIVALKAVYEVWNNAYAVLNVEYNNARGYDVVSDPIEGENRLTAQGYLDA
                     FSPKFYQGENWTLTVGFSIGF"
     gene            3890..>4780
                     /locus_tag="PK641_04630"
     CDS             3890..>4780
                     /locus_tag="PK641_04630"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPG55666.1"
                     /translation="MKKIQMVDLKSQYEKIREDVNNGIQEVIDTTTFIKGGKVNDFQK
                     NLEAYLGVKHVIPTGNGTDALQIALMALGLKPGDEVITPTFTFIATAEVVALLGLKPV
                     VVDVEEDTFNISIESIKKAITTKTKAIVPVHLFGQCANMEAILEIAKEHNLFVVEDAC
                     QAIGAEYTFKDGSKKQAGCIGNIGCTSFFPSKNLGCYGDGGAIFTNDDDLAARMRAIA
                     NHGMVVQYHHDFVGVNSRLDSIQAAVLNAKLPHLNEYIAARQKAAATYDKAFAGNKHI
                     ITPVHFASSTHVFHQYTLKLV"
BASE COUNT         1697 a          659 c          840 g         1584 t
ORIGIN      
        1 caatcaaaat atgatggtaa agatttgtgg aaaaaatacg actatcatga ttttggaata
       61 attggtgaac cttattttga tactgatttt tgtgactttt tctatataac agatacaggt
      121 agaatgtggg ataggtataa tgttagtgta cgcgataaaa tacctgttca tcaagataga
      181 tggattagtc aaggtcttgt atatcattat actaaggata tatgtaaagc aattgattta
      241 ggaacattcc cgaaacgcat gatgattacc acacatcctc aaagatggac caataataca
      301 atagaatgga tgagagaatt attgcttcag aatgttaaaa atgttataaa attcctaatc
      361 aaacgaatga aaaaaagtat aagtagttta cattgatttt taacaagatt atgacgtgtt
      421 aagttgaatc aatgatatat ctttgtaaag tttacttttt agtactatag ccccttacaa
      481 gacattttgt aaaataaata ttgagttaag aattaacaaa tggttgtgta tggtattaat
      541 tttacatgaa aaacaaaata ttttactttc ctaattataa gctgatctta cttgatttag
      601 taggcttgct tctgagttat gtcatagtaa ttggtataac acctactcat gcagaaaatc
      661 catgggctaa atacttgcct attataggca tatacgcttt tctttggttt ttctttggct
      721 atttatttag gcggtatgta ggtagattaa aaaatagagg ttaccttaaa actgctttca
      781 attctttata ttgttcattt gctgcttatt taattagtct gatattgttt ttgtatgtac
      841 ttaacggata ctccattagt gtgttgtctg tttttacaat aatagcattc gccatatcat
      901 ttataggcga tacaatctat tttgcatata tgtacgctct tgatccggaa gaagttcaac
      961 cgatctcagt tcgtgaacac aagtcagtaa ttacaaaacc attcacatta gacgaagaag
     1021 catatagtaa tagagagcat gctatattaa atttaaccgg aaaaaaatct ctcgagtatt
     1081 tttctgagtt tgtcgactta agaagttcta atacaaatgt attatctaca actcaattat
     1141 ttaatgtaga acaaataaga ccatataatt ttgatgttat tattaactta cgtagtttaa
     1201 acaatattag aggcatcaat aagatgtttt gtttaatcaa tgaaaaacta cctgataacg
     1261 gcatttatat tggttgtttt aaagataaaa gtgtaaaaaa gaaacaaata ttagacaaat
     1321 atccaaaagg aataaattgg gtaatctata caatgtatta cataattaaa cgcttgcttc
     1381 ccaagttgtt tataacccgt catctttatt atgatataac tcaaggcaaa aagagagtgt
     1441 tatcaaaaac agaagtcttt ggacgtttgt attattgtgg atttgaagtt ttagatgaaa
     1501 agaaaataga atgttataca tatttcatag ctcgtagaaa acaatctcca ccggcacgta
     1561 aaaaacgtag atatggaccc attattgcac tgaatagagt aggtaaaaat ggtaaagtgt
     1621 tcaaatttta taagatgcgt accatgtatc cgtattctga gtttttacaa gactacatat
     1681 accaaaaatg cagcttacaa gaaggaggaa aattcaatca tgatatacgt gtaaatacta
     1741 taggtcgaat catgagaaag ttttggatag acgaacttcc gatgatgata aaccttatta
     1801 aaggagatat gaaactagtt ggcgtaaggc cacttagtaa tcagtatttt aatttatatt
     1861 gtgatgagct acagaaaatg agggttaaat tcaaaccggg tttactacct ccattttatg
     1921 ctgatatgcc taaaacttta gatgaaatcc aagcttctga gatgaaatac ttgagattat
     1981 gcgaaaaaca tggatatttt gttaccgata ttcagtacct ttggaaaatt tttgcaaata
     2041 tagttttcaa acacgccaga tcacactaaa aattcgaatg aaacgatata aatcattgat
     2101 atttgcactc tgctgttttg tttcagcttt agctcaagat attcctgaac acatatcata
     2161 tacgcgtgtt tacgattttg ttgatgagtt ggcaattgat ggtatagttg aagtcaactc
     2221 tgtaattaag ccgtatagca gaaattttat tgctcaaaaa ttagcagaag caagcaaaaa
     2281 agacagtctt cttaataagc gtcagaaatc tgatttaaaa ttctttatga gtgaatttgc
     2341 tatcgaatta gatacgatgc ctaaagctta tgttcattgg acagataaaa aatctttcga
     2401 tttatcgctt cttcagcctc agttcatgta tcgtgataaa tattttaaat gtcaagtaag
     2461 acctattata ggtatggatc tactatataa taggcatgga cttattatga aacgttggta
     2521 tggagccgat attcaaatgg atattgttaa acatgtatct gtatggggta gtattagaga
     2581 tatctcattc aatggggatt atttgaatga cacttatttt cctgaccagt atgcaaaaat
     2641 aaatggagca aaaatttcac aatcaggttt tttgaatgat ttacccggat gtgcatacaa
     2701 agaatctaat agtggtggcg attacagtga ttttagaggt ggtattaaag cttatgtatg
     2761 gtggggaagt ataggtttgg taaaggataa tattgtttgg ggtgataatt atcatggttc
     2821 taatattata tcaggaagag ccccatcgtt tccaatgata actttgaatt taaaaccatg
     2881 caagtggttt gagtttaatt atatacatgg atggatggta agcaatgtga ttgatagttc
     2941 ttattattat aacgaagaac agtatacaga ttcaacatct aaacggcatt ataggccaat
     3001 gaacaagttt attgccgcta atatgttcac gtttacacca tatccaaaat taaatatatc
     3061 aataggaaat tcaattattt acgcagaaag gagcgtacaa gcaatttact ttataccatt
     3121 cgcatttttt aaatctttag atcatttaat gaccaaagat ttacgtgtcg aaaatcaaaa
     3181 ttcacagata tttatcaatg taagttcgcg taatataaag catttacatt tatatgcatc
     3241 attctttata gatgaattta gcattaacag gtggaaaccg gataataaag aacgtaactt
     3301 agtatcatat aaagcaggat ttaatttaag caattggccg gttaagaatc ttgcagtaaa
     3361 ttttgagtat acacgtagca atatattaac ttataaacat tctattgatg ctattacttg
     3421 ggcatctaac gattataatt taggtcacta tttaggcgat aattctcaag aattttttgt
     3481 gtcagttaat tacaaaccta ttcggagttt aaatcttaac ttgtcttata ctaattctca
     3541 aaaaggcagg gattataaat atgtaaggac taatacaaaa gaaattttga gccaagacgt
     3601 tttaaacgaa tttacatata aaaacgaaat agttgcctta aaagctgttt atgaagtatg
     3661 gaataatgcc tatgcagtat taaatgttga atataataat gcaagaggct atgatgtagt
     3721 atctgaccca atagaaggag aaaatcgttt aacagcgcaa ggatatttag atgcattttc
     3781 accaaagttt tatcaaggtg aaaattggac actaacggta ggattctcga taggattcta
     3841 aaaaaaagaa attttatttt ttttgattta ataattaata cagattagta tgaaaaaaat
     3901 tcaaatggtt gatcttaaaa gtcaatacga aaaaatccgt gaggatgtca ataacggcat
     3961 tcaggaagta attgacacaa caacctttat taaagggggt aaggttaacg atttccagaa
     4021 aaatctggaa gcttatctgg gagttaagca tgttattccc acaggaaacg gtaccgatgc
     4081 tctgcaaatt gctttaatgg cattgggttt aaaaccgggt gacgaggtta ttactccgac
     4141 atttactttt attgcaacag cagaagttgt tgctttatta ggcttaaaac ctgttgttgt
     4201 agatgttgaa gaagatacat tcaacattag tatagaatct attaaaaaag ctattacaac
     4261 taaaacaaag gcaatagttc ctgtgcattt gtttggtcaa tgcgcaaata tggaagctat
     4321 acttgaaatt gcaaaagaac ataatttgtt tgttgttgaa gatgcatgcc aagctatagg
     4381 tgcagaatat acatttaaag acggcagtaa aaaacaagcc ggttgtatag gtaatatagg
     4441 ttgtacatca tttttccctt ctaaaaattt aggatgctac ggagatggtg gtgcaatatt
     4501 tacaaatgat gatgatttag cagctcgtat gcgtgctata gcaaaccatg gtatggtagt
     4561 ccaatatcat catgattttg ttggagtaaa ttctcgctta gacagcattc aagcagctgt
     4621 acttaacgct aagttacctc acctaaacga atatatagca gcacgccaaa aagcagctgc
     4681 aacttatgat aaagcatttg caggtaataa acatattatc acaccggtac attttgcttc
     4741 atcaactcat gtatttcacc aatatacatt aaaattagtt
//
LOCUS       DAOPID010000110         4665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_24728_length_4665_cov_3.707592, whole genome shotgun sequence.
ACCESSION   DAOPID010000110 DAOPID010000000
VERSION     DAOPID010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24728_length_4665_cov_3.707592"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1743)
                     /gene="dxs"
                     /locus_tag="PK641_04635"
     CDS             complement(<1..1743)
                     /gene="dxs"
                     /locus_tag="PK641_04635"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HPG55667.1"
                     /translation="MSNKYPLLNTIDYPSDLRKLDKSQLQQVCDELRQFIIDQLSCNP
                     GHLASSLGAVELTVALHYVFDTPNDRIVWDVGHQAYGHKILTGRRCVFNTNRKFKGIS
                     GFPNPKESEYDSFIAGHASNSVSAALGISVADEMQHNHHKVIAVIGDGAMTGGLAFEG
                     LNNVCVHPNNLLIILNDNNMSIDKPVGGISQYLMDITTSRLYNRLRFKTHHLLKKLGL
                     MNDNKKNRIVRFANSIKNTVSGSHNIFEGLNIRYFGQLDGHDINQLVKVLNDIKNFDG
                     PRVLHIKTKKGKGFAPAEQEATIWHAPGLFNPETGELIKSEDVKNISLYQDVFGETLS
                     ELARKNDKIVGITPAMPTGCSMNIMMKKMPERVFDVGIAEGHAVTFSAGLAKEGMMPF
                     CNIYSSFMQRAFDNLIHDVAIQKLNIVLCLDRAGIVGADGPTHHGAFDISYLRCIPNL
                     NISSPLNEYELRNLLYTAQLPGKGPFVIRYPRGKGVGVEWENQEMKEIPVGTGKMMKD
                     GSKIAVLTIGHIGNVAKDAISSLKDDADKFAWYDMRFVKPLDTKLLDEIAQKFKQIVT
                     IEDGSLKGGFGSAVL"
     gene            1904..2878
                     /locus_tag="PK641_04640"
     CDS             1904..2878
                     /locus_tag="PK641_04640"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPG55668.1"
                     /translation="MKTIEKTILILFIAISAASCVTQEKYNEAKESEARYYDEARRCG
                     ESLASANVKNKELEAELTRLKALKAKADADTAKLSKELRRVQDDCNNMQEQNRALLEK
                     LQTSKSKEEAQAMLAEIQALQSELIKREDALFQAERQLSDKQKELELRNAKISELTDL
                     IADKERKMQEIKEKIKKALTSYEGDGLQITTKNGRIYVSLDEQLLFQSGKWDVDARGA
                     AAISKLSTVLAQHKDINVMVEGHTDNVPYNGSGNILDNWDLSVKRATAIVRILLKSGD
                     LEPERVIASGRSEYNPVDTDNTSDARKRNRRTEIILSPNLDELMKIFE"
     gene            2878..3810
                     /locus_tag="PK641_04645"
     CDS             2878..3810
                     /locus_tag="PK641_04645"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HPG55669.1"
                     /translation="MDFRTIVQLPNNAPNINHKDSWLLLGSCFAQNIAKKASFYGFES
                     LYPFGALYNPISIIKSLNMLKDNVRLTDADLFLEREQWNSYNFHSDYSDIDKNVALSK
                     MNECLDKAHKILESAKIIIITFGTAYIYQNNYTGEIVANCHHSVMSKFTRKLLTADEI
                     VLSYQDILNKYSEKQFIFTVSPIRHLKDGAHANQISKSTLLLAIDNICNTYKNCHYFP
                     SYEIVLDELRDYRFYSDDMIHPSKTTVDYIWERFSETYFNKETQNLNKQIEEIRLEEM
                     HRPILPDSISHKQRIAILKQKKDELIQKFPYIHW"
     gene            3819..4538
                     /locus_tag="PK641_04650"
     CDS             3819..4538
                     /locus_tag="PK641_04650"
                     /inference="COORDINATES: protein motif:HMM:NF041646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VC0807 family protein"
                     /protein_id="HPG55670.1"
                     /translation="MDKEQKTQKKENSLISILVSIVIPAIILSKFSKPEYLGVLPGFL
                     IALAFPIIQSLYDIIVNKKAGLIAIIGFVSIFLTGIIGVLELPSEWMAYKEASVPLLI
                     GIAVVVSLKTPYPLIKKLFYNEDLIDVEKIDAILIENNSKEKFEKIMRNATYMIGASF
                     LLSAILNFILTKIIIQSPTGTEAFNEELGRLTALSYPVIALPSTLVMFVALWYLFKQL
                     KKITNLSIEEMMSESLKNKSK"
BASE COUNT         1666 a          812 c          734 g         1453 t
ORIGIN      
        1 taatacagca cttccgaagc ctccttttaa agaaccatct tcaattgtaa ctatttgttt
       61 gaatttttga gcaatttcat cgagtaattt agtatccaat ggctttacaa aacgcatatc
      121 ataccatgca aatttatctg catcatcttt caatgatgaa atcgcatctt ttgcaacatt
      181 acctatatgt cctattgtta gtactgcaat tttagaacca tctttcatca ttttgcctgt
      241 acctacaggt atttctttca tttcttgatt ttcccactct actcctactc cttttccacg
      301 cggatatctt attacgaatg ggcctttgcc aggtaattga gctgtatata acaaattacg
      361 caattcatat tcgtttaatg gtgaagaaat gtttagatta ggaatacatc ttaaatatga
      421 tatatcaaat gcaccatgat gagttggtcc atcagcccca actattccgg ccctatcaag
      481 acacaataca atgttgagtt tttgaatagc cacatcatgg attaggttat caaaagctct
      541 ttgcataaaa gacgaatata tattgcaaaa tggcatcatt ccttcttttg ccaaacctgc
      601 cgaaaaagta acagcatgac cttctgctat tccaacgtca aaaacgcgct caggcatttt
      661 tttcatcatt atattcatcg agcatccggt tggcatagca ggagtaattc caactatttt
      721 atcgtttttg cgagctaatt ctgacaatgt ttctccaaaa acatcttgat ataaagatat
      781 atttttgaca tcttcacttt taattagttc accggtttcg ggattaaata atccgggtgc
      841 gtgccatatt gtagcttcct gttcggcagg agcaaaacct ttaccctttt tagtctttat
      901 atggagtaca cgaggaccat caaaattttt aatatcattc aataccttca ccaattgatt
      961 gatatcatga ccatctaatt gtccaaaata cctaatattg agtccttcaa aaatattatg
     1021 agaccccgac actgtgtttt ttatggaatt tgcgaaacgt acaatacgat ttttcttatt
     1081 gtcgttcatc aaacctaatt tctttaatag atgatgggtt ttaaaacgta gacgattata
     1141 taacctagat gtggttatat ccataagata ttgacttatt cctcctaccg gtttatcaat
     1201 cgacatattg ttgtcattca aaataatcaa aagattatta ggatgcacac atacattatt
     1261 cagaccttca aaagcaagcc caccagtcat agctccatca cctattacag caattacttt
     1321 atgatgatta tgctgcattt catcggcaac tgagattcct aatgccgctg atactgagtt
     1381 agaagcatgt cctgcaataa acgaatcata ttcactctct ttaggattag ggaaaccact
     1441 aataccctta aattttcgat tcgtattaaa tacgcatctt cgtccggtta aaatcttgtg
     1501 tccataagct tgatgaccga catcccatac tatacgatca ttaggagtgt caaatacata
     1561 gtgcaaagca acagtaagtt caacggctcc caaactagat gctaaatgac ccggattgca
     1621 tgacaattgg tcaataataa attgtcttaa ttcatcgcac acttgctgta gctgtgattt
     1681 gtctaatttg cgtaaatcag acggataatc tatcgtgttt aacagtggat atttattgct
     1741 cataaaaata cgggcagaac ttttttattc gtttaatcaa atttaggtgc aaatatacaa
     1801 tttaaattat gcaattaatg attattaatt gtaatttatt caacaagcga ttgggttttc
     1861 tttacatttg caaaacataa accaaaataa gatgatttaa tccatgaaaa caattgaaaa
     1921 gactatttta attttattta ttgctatcag tgcagcatct tgtgtaactc aagaaaaata
     1981 taacgaagca aaagaaagtg aagctcgcta ctacgatgaa gcccgcagat gtggtgaatc
     2041 tttagcatca gcaaatgtaa agaacaaaga attagaagca gaattgacca gactaaaagc
     2101 tctaaaagcc aaggctgatg cagatacagc caaactatca aaagaattac gtagagttca
     2161 agatgattgt aataatatgc aagaacaaaa tcgtgcactt ttagaaaagc tacaaacatc
     2221 aaaaagtaaa gaagaggcac aagccatgtt ggcagaaatt caagcactac aatctgagtt
     2281 aattaaacga gaagatgctt tatttcaagc agaacgtcaa ttatcagata agcaaaaaga
     2341 attagagctt agaaatgcca aaatatcaga attgaccgac ttaattgctg ataaagaacg
     2401 taaaatgcag gaaattaaag aaaaaattaa aaaagcattg acaagttacg aaggagacgg
     2461 tcttcaaatt actactaaaa acggtagaat ctatgtatca ctcgacgagc aactattgtt
     2521 tcagtcagga aaatgggatg ttgatgcacg tggtgctgct gcaatcagta aattatctac
     2581 agttttagct caacataaag acatcaacgt catggttgaa ggacatactg acaatgtgcc
     2641 atacaatgga agtggaaata tattagacaa ctgggattta agtgtaaaac gtgcaaccgc
     2701 tatagtacgt attttactta agagcggaga tttagaacct gagcgtgtaa ttgcttcagg
     2761 tagaagtgaa tataatccgg ttgatacaga taatacttct gatgcaagaa aacgtaatcg
     2821 ccgtactgaa atcattttaa gtcctaactt agatgaattg atgaaaattt ttgaataatg
     2881 gattttagaa ccattgtaca attaccaaat aatgcgccta atatcaacca taaagacagt
     2941 tggttgttat taggctcatg ttttgcacaa aatatcgcaa aaaaagcctc attttatgga
     3001 tttgagtctt tgtatccctt tggtgcattg tacaatccta tttcaataat aaaaagtctg
     3061 aatatgctaa aagacaatgt ccgtttaaca gatgcagact tattccttga acgtgagcag
     3121 tggaacagtt ataattttca tagtgattat tcagacatag acaaaaatgt tgcactctct
     3181 aaaatgaatg aatgtttgga taaagctcac aaaattttag aatctgctaa aataatcatc
     3241 atcactttcg gtacagcata tatttatcaa aataactata caggcgagat cgttgccaat
     3301 tgtcaccatt ctgttatgag taaatttaca cgaaagttac ttacagcaga cgaaattgtt
     3361 ttatcatatc aagatatact gaataagtac tcagaaaaac aatttatatt tacagttagc
     3421 cctatcagac atttaaaaga tggagcccat gctaatcaaa taagtaaaag taccttgcta
     3481 ttagctattg acaacatttg caacacatac aaaaactgtc attatttccc ttcgtacgaa
     3541 attgttttag acgaattgcg agactatcgt ttttattcag acgacatgat acatccttca
     3601 aaaacaacag ttgattatat atgggaacgc ttttccgaaa cgtattttaa taaagaaact
     3661 cagaatctaa ataaacaaat tgaagagata agattagaag aaatgcaccg ccctatattg
     3721 cctgatagca tttcgcataa acaaaggatc gccatattga aacaaaaaaa agatgaattg
     3781 atacagaaat ttccttacat acattggtaa aaaaactcat ggataaagaa caaaaaacac
     3841 aaaagaaaga aaattcactg ataagcattt tggtcagcat tgttattcct gctattattc
     3901 tgtcaaaatt tagcaaacct gaatatttag gcgttctgcc cggattttta attgcattag
     3961 ccttccctat catacaaagt ttatacgaca ttatagtaaa caaaaaagca ggtcttattg
     4021 caattattgg ttttgttagc atcttcctaa caggtatcat tggagtttta gaacttccat
     4081 cagaatggat ggcatacaaa gaagcttctg taccattact tataggtatt gcagttgtgg
     4141 tatcactcaa aaccccatat ccattaatta aaaaattatt ttataacgaa gatttaattg
     4201 atgttgaaaa aattgatgca attttgattg aaaacaattc gaaagaaaaa tttgaaaaaa
     4261 ttatgcgtaa cgctacatac atgataggcg catccttttt attgtcagca atactaaatt
     4321 ttatattaac caaaataatt attcaaagcc ctaccgggac tgaagcgttt aacgaagaac
     4381 taggcagact aacagcattg agttatcctg taattgcact accgtcaaca ttagtaatgt
     4441 ttgtagcttt gtggtactta tttaagcaat taaaaaagat tactaactta tcaattgaag
     4501 aaatgatgtc tgaatcttta aaaaacaaaa gcaaatgaat tacgatataa aagacattgt
     4561 caaacttatt aattgtaaag caataattaa ttgcgaatac tctaatatta agtacatatt
     4621 aacagatagt cgcaaattaa tatggccaca agagacaatt tttac
//
LOCUS       DAOPID010000111         4649 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_24852_length_4649_cov_3.792991, whole genome shotgun sequence.
ACCESSION   DAOPID010000111 DAOPID010000000
VERSION     DAOPID010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4649
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24852_length_4649_cov_3.792991"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="PK641_04655"
     CDS             <1..586
                     /locus_tag="PK641_04655"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55671.1"
                     /translation="AGTTYKYYGKACLLPEEGGVVDGNWTTQWKDGEISLLQDNENAV
                     IGCGGEANKLGLISESVVYIEVAQWQSVPCVPDEEYNISFKAPACTPEDAQVYIIGSF
                     KESTWGTAVPMVLNGEGNWTATVTGQATSEYKFRLTESSWDDQLQEYVVNVETEVGEW
                     KDCANKSLGDNTEIFFDASNAEQYKWTSCEAPVE"
     gene            728..1582
                     /locus_tag="PK641_04660"
     CDS             728..1582
                     /locus_tag="PK641_04660"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPG55672.1"
                     /translation="MRKGNKLSFFKRIRVKYKLSVLNEGTLEEVYHFRLSALSFFVLS
                     MVILLLAMALFSLLIWVTPLRKLLPENVDMTLRKEVVEDAMKIDSLTREVEMRQVYLN
                     TIKEVVMGNIPIDTTITSDSLMAQQREEVLMEKTAEEKEFCKNFEDEERYNLATTTNA
                     TTDMVFFRPVKGIITGKFTPVQNHFGIDISTQPNATISAVYQGVVLTSSYDPQEGYFI
                     QILHKQNFISVYKGASMVFKKTGDVVNTGEVIAIVGHGDKKDKPSLHFELWSGMQPQD
                     PSNYIVLE"
     gene            1579..2739
                     /locus_tag="PK641_04665"
     CDS             1579..2739
                     /locus_tag="PK641_04665"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HPG55673.1"
                     /translation="MKKRIAILGSTGSIGTQALDVISAHPDLFEVYALTANNNVDMLI
                     EQARRFKPLFVVITNEQHYDRLKNALADEDIKVFAGMQSICELVEMPSVDMVLTAMVG
                     YAGLLPTIHAIKASKTIALANKETLVVAGELICSLAQNYNSTIIPVDSEHSAIFQCLS
                     GEGDNKIEKILLTASGGPFRTKTLEELQHVTKSDALKHPNWSMGDKITIDSASMMNKG
                     FEVIEAKWLFGVESSQIEVVVHPQSIIHSMVQFADGAVKAQLGMPDMRLPIQYAFAYP
                     NRLENSYSRLDFFDLANLSFERPDLNKFRNLGFAYEALKRGGNMACIINASNEVVVSA
                     FLQGKIGFLQMSDVIEFAMNKVPFIEKPTYEDYVATDSETRKLVAEHKYLNI"
     gene            2759..4075
                     /gene="rseP"
                     /locus_tag="PK641_04670"
     CDS             2759..4075
                     /gene="rseP"
                     /locus_tag="PK641_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HPG55674.1"
                     /translation="MQLLIQILQLLACLSLLVIVHEFGHFITAKIFGVRVEKFYLFFN
                     PGFSLLKWKPKNSETEYGIGWLPFGGYVKLAGMIDESLDTDQMKKPVQDWEFRSKPAW
                     QRLIIMLAGVVMNFIAAIVIYSMVLFTYGESNVPMREAKLGMDFSEVAHAQGFKDGDI
                     ILSADGDSLVYFDEESMRKIIGASQVVVLRDGKEHTLTMTSTFMKDMIASKQGFASYR
                     IPFVVREVINGGPAESAGLQVGDSIVGVNDTVIITMDDSYAIFQNNKNNVIKLNFYRA
                     GLEDSVSLALDSAGRIGVYVKPYFEVYPTVNYEYGFFESFPAGIEKGVKTFTGYGSDM
                     KMVFSKEGVESLGGFGTITSLFPGNFSWAAFWYLTAYISVILAFMNVLPIPALDGGHI
                     LFLLYEMITRKKLSQEFMIRMQKIGLFILLLLLLYANGMDIFRAFK"
     gene            complement(4146..>4649)
                     /locus_tag="PK641_04675"
     CDS             complement(4146..>4649)
                     /locus_tag="PK641_04675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPG55675.1"
                     /translation="SKTDETIPYEGGSFNGSIENTVKSWINKNAHSDPNIYQIGSGVE
                     GTTIYDYSESPRVLFYKIEEASHTLSDVHGFDPNTEIWKFFNNIPLSVDDAKTDKLDI
                     FPNPADDILYTSEDGMYSITDLSGRILKKGYTDEYSIDLKGLSRGRYIVGVTTDTETY
                     KAVLLKK"
BASE COUNT         1473 a          667 c          993 g         1516 t
ORIGIN      
        1 tgctggtact acttacaagt attatggtaa agcttgcttg cttcctgaag aaggtggtgt
       61 agttgacggt aactggacaa cacaatggaa agatggagaa atttctttat tgcaagataa
      121 tgaaaatgca gttattggtt gcggcggtga agccaataaa ttaggtttga tttctgagtc
      181 ggtagtttac atagaagttg cacagtggca atcagtacct tgtgtacctg acgaagagta
      241 caacatttct tttaaagctc ccgcttgcac accggaagat gctcaagttt atatcattgg
      301 ttctttcaaa gaatcaactt ggggtacagc tgttcctatg gtattaaatg gagaaggaaa
      361 ttggacggca accgtaacag gtcaagctac atcagaatac aaattccgtt tgacagaatc
      421 aagttgggat gatcaattac aggaatatgt agtaaatgtt gaaactgagg ttggtgaatg
      481 gaaggattgc gctaacaaat ctttgggcga caatacagaa atcttttttg atgcttcaaa
      541 tgctgaacaa tacaaatgga cttcatgcga agctcctgta gaataatctt ataagattat
      601 aaaatataaa aaggttcatc tcgcttttgc ggggtgaact tttttgttgt ttcccagcca
      661 aaaataaatt ttcatttact ctcggcttgc actatctttg tccagttaat ttgagattat
      721 gaataaaatg cgaaaaggta ataaactaag ttttttcaaa cgaataagag taaaatataa
      781 gttatccgta cttaacgagg gaactttgga agaggtgtat cactttagat tgtcagccct
      841 tagttttttt gtactctcaa tggtaatatt attacttgcg atggctttgt tttcactttt
      901 gatatgggta acaccattga ggaaattatt gcctgaaaac gtagatatga cattaaggaa
      961 agaagtagtt gaggatgcta tgaaaataga ctctttaacc cgggaagttg aaatgcgaca
     1021 agtttatttg aatacaatta aggaagttgt tatgggtaac attcctattg atactacaat
     1081 aacttcggat tcgttgatgg cgcagcaaag ggaagaagtt ttaatggaaa aaacagcaga
     1141 agaaaaggag ttctgtaaaa actttgaaga tgaagaaaga tataatctgg ctactactac
     1201 aaatgcaaca acagacatgg tgttttttag acctgtaaag ggtattatta caggaaaatt
     1261 tacaccggtg cagaatcatt tcggtattga tatatcaact cagccaaatg caactatttc
     1321 ggctgtttat cagggtgttg tcctgacatc gtcttatgat cctcaagaag gctattttat
     1381 tcagattttg cataaacaga attttatatc agtttacaaa ggggcatcca tggtatttaa
     1441 gaaaacaggc gatgtggtta ataccggtga ggttattgca attgtaggac atggagataa
     1501 aaaagataag ccttcgttac attttgaatt gtggagtggt atgcaaccac aggatccaag
     1561 taattacata gtattagaat gaaaaaacga atagcgatat taggttcaac cggttctatt
     1621 ggaactcagg ccttggacgt aatttcagcg catcctgatt tgtttgaagt atacgcactg
     1681 acagcaaaca ataacgttga tatgctaatt gagcaggcac gtcgttttaa accgctgttt
     1741 gtagtaataa ccaacgaaca acattatgat agattaaaga atgcattggc agatgaagat
     1801 ataaaagtgt ttgccgggat gcaatctatt tgcgaacttg ttgagatgcc gtctgttgat
     1861 atggtattaa cagctatggt tggctatgca ggcttattac ctactataca tgctattaaa
     1921 gcaagtaaaa caattgcttt agcaaataaa gaaacattgg ttgtggccgg tgagttaata
     1981 tgctctttag cacaaaatta taatagcaca attattcctg ttgattctga acactcagca
     2041 atatttcagt gtttgtcagg tgaaggggat aataaaatag aaaaaatatt acttacagct
     2101 tccggtggtc catttaggac aaaaacactt gaggaattgc agcatgtcac taaatctgat
     2161 gcattaaagc atccaaattg gtcgatggga gataaaatta ctattgattc tgcaagtatg
     2221 atgaataaag gttttgaggt aattgaagcc aaatggttgt ttggagttga gagtagtcag
     2281 atagaagtag ttgtgcatcc gcagtctatt atacattcga tggtgcagtt tgcagatggt
     2341 gcagtaaagg ctcaacttgg tatgcctgat atgcgtttgc ccatacaata tgcttttgca
     2401 tatcctaatc gtcttgaaaa ttcatattcg cgtcttgatt tttttgattt agcaaatttg
     2461 agttttgaac gtcctgattt aaacaagttt agaaatttag gatttgctta cgaggcattg
     2521 aaacgaggtg gaaatatggc atgtataatt aatgcttcga atgaagttgt agtatcggca
     2581 tttttacaag gaaagatagg tttcttacaa atgagtgatg taattgaatt tgcgatgaat
     2641 aaagttccgt ttattgagaa gccgacttat gaggattatg tggctactga ttcggaaacg
     2701 cgtaaattag tagctgaaca taaatattta aatatataat taccaaaaca tttttaatat
     2761 gcaattgttg attcaaatat tacagttgtt ggcatgtttg tcgttattgg ttattgttca
     2821 tgaatttgga cactttataa ctgcaaaaat tttcggagta agagttgaaa aattttattt
     2881 gttttttaat cccggttttt cattgctcaa atggaaacca aagaacagtg aaactgaata
     2941 tgggataggg tggttgccat ttggcggcta tgtaaaactt gcaggaatga tagacgaaag
     3001 tcttgacact gatcaaatga aaaagcctgt tcaggattgg gaatttagat cgaagccggc
     3061 atggcaaaga ttaataataa tgttggcagg ggtagttatg aattttattg ctgccatagt
     3121 tatttactct atggttttgt ttacttatgg cgagagtaat gttcctatgc gcgaagctaa
     3181 gcttggaatg gatttttcgg aggtagccca tgcacaagga tttaaagatg gggatattat
     3241 attatcagca gacggagata gtcttgtgta ctttgatgag gaatcaatga gaaaaatcat
     3301 tggtgcatcg caagtagttg tgttgcgaga tggtaaggag catactttga caatgacaag
     3361 tacatttatg aaagatatga ttgcatctaa gcagggattt gcatcatatc ggattccttt
     3421 tgttgtacga gaagtaatta acggagggcc tgctgaaagt gccggattgc aagtgggaga
     3481 tagtattgtg ggtgtaaatg atactgtaat aattacaatg gatgattcgt atgctatatt
     3541 tcaaaataac aaaaacaatg taataaaatt aaacttttat cgtgccggat tggaagattc
     3601 agtttcattg gctttggatt ctgccggacg cataggtgta tatgttaaac cttattttga
     3661 ggtatatcct actgtaaatt atgaatatgg cttttttgaa tcatttcctg ccggcatcga
     3721 aaaaggtgta aagacattta ccggatatgg tagcgatatg aaaatggtgt tttcaaaaga
     3781 aggagtggag agtttgggcg gttttggtac aataacaagt ctatttcccg gaaatttctc
     3841 atgggcggca ttctggtatc taacagctta tatatctgta atattggcat ttatgaatgt
     3901 tttaccaata cctgctttag atggtggaca tatattgttc ttgctttatg aaatgattac
     3961 aagaaagaaa ttaagccaag agtttatgat aagaatgcag aaaataggat tgtttattct
     4021 gttgttatta ttactctatg ctaacggaat ggatatattt agagcattca aataatattt
     4081 aagtgaaata aaaaagcagg cgaattcaat tgagttcgcc tgctttttgt atgatataag
     4141 ctaatttact tttttaataa aacagcttta tatgtttcgg tatcggttgt tacaccaaca
     4201 atgtaacgtc ctcttgaaag gccttttaag tctatgctgt actcatcagt atatcctttc
     4261 ttaagaattc ttccgcttaa atccgtgatc gagtacatgc catcttctga tgtgtacaat
     4321 atatcatctg caggattggg gaaaatatca agtttgtctg tttttgcatc atccacactc
     4381 aaaggaatat tattgaagaa tttccatatt tctgtattcg gatcaaagcc atgtacgtca
     4441 gaaagtgtgt gtgatgcttc ctcaatttta taaaacaaaa cccttggtga ttcgctgtag
     4501 tcgtatattg tagttccttc aacaccggaa cctatctgat atatgttagg atctgaatgt
     4561 gcgttcttat taatccatga tttaacagta ttttcgattg atccattgaa tgatccgccc
     4621 tcgtatggaa tagtctcatc tgttttgct
//
LOCUS       DAOPID010000112         4631 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_24982_length_4631_cov_3.867570, whole genome shotgun sequence.
ACCESSION   DAOPID010000112 DAOPID010000000
VERSION     DAOPID010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24982_length_4631_cov_3.867570"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /gene="atpD"
                     /locus_tag="PK641_04680"
     CDS             complement(<1..1065)
                     /gene="atpD"
                     /locus_tag="PK641_04680"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HPG55676.1"
                     /translation="MAQINGSITQIIGPVVDVYFEEGGDNLPKIHDALQITRSNGLKL
                     ILECQQHIGENSVRAIAMDSTDGLVRGMEVTPTGAPISMPIGNQIKGRLLNVTGDAID
                     GIGSIDRSNEYPIHREPPKFEDLATSKEILYTGIKVIDLLEPYLKGGKIGLFGGAGVG
                     KTVLIMELINNIAKGYGGYSVFAGVGERTREGNDLLREMLESNVINYGEEFKKSMEAG
                     DWDLTKVDPKALEESKITLVFGQMNEPPGARASVALSGLTVAESFRDANAKDGKGNDI
                     LFFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGRMQERITSTKNGSITSVQA
                     IYVPADDKTDPAPATTFSHLD"
     gene            complement(1243..1572)
                     /locus_tag="PK641_04685"
     CDS             complement(1243..1572)
                     /locus_tag="PK641_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55677.1"
                     /translation="MKKLLLILLLCLPISIFAKVSIDKDNCTITKDGKTFPLYGKVKI
                     VESFPDIKVKIVDSFADIKVTIVDSFADECGKVKIVDSFPTIKVQLVNSFPDITVKIV
                     DSFPGIK"
     gene            1869..2030
                     /locus_tag="PK641_04690"
     CDS             1869..2030
                     /locus_tag="PK641_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55678.1"
                     /translation="MKDKLKDLLFLKALLASNKELVEMKNKVRKQFGEPTIEEEIAIL
                     EKELKEEGK"
     gene            2291..2668
                     /locus_tag="PK641_04695"
     CDS             2291..2668
                     /locus_tag="PK641_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPG55679.1"
                     /translation="MFSIRKSAIILSFLAIIISVNAQTYTSLNNEDFAKQLKNKKLQL
                     VDVRTPSEYAAGHLPKAINIDFKNQDFEKNILKLNKKKKVAVYCRSGVRSKKAAEILS
                     KNNYIVIELNTGISKWNGDIVTK"
     gene            2899..3873
                     /locus_tag="PK641_04700"
     CDS             2899..3873
                     /locus_tag="PK641_04700"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HPG55680.1"
                     /translation="MKIIGTGKALPSLSVTNDMLAEFLDTSDNWISTRTGIKERRLLS
                     KDNLLGLTTDASRLALENAGITAADLDYIICSNVANKYVTPALSCILQGEINATCPCI
                     DINGACAGFIYALDIAESFLQTKPYVNNVLVVCAEEPSRFTNWKERSTSVLFGDGAAA
                     VILSKGDDLKSALLTTTSNVDALYYQRDLEDSPYVEKKIDAAPLVMNGKDVFKLAVAS
                     SVADISEVVEKAGLTFKDIDYFMLHQANIRIIDAICNQLQQPEEKFPHNIERYGNTSS
                     ASIPILLDEMNRDGRLKKGSKIVLSAFGAGFTTGACVIEWSKSSNEKT"
     gene            3888..>4631
                     /locus_tag="PK641_04705"
     CDS             3888..>4631
                     /locus_tag="PK641_04705"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HPG55681.1"
                     /translation="MFRSRICDLVGIKYPIFQGGMAWVSESILAASVSNAGGLGIISA
                     GNADANYVREQIRKCKKLTDKPFGVNVMLLSPFAAEVAEVLAQEKVAVVTTGAGNPGK
                     YMQMWLDAGIKVIPVVPSVALAQKVESIGACAVIAEGGESGGHIGDLATITLVPQVCH
                     AVKVPVIAAGGIADGRGMAAAFILGAEGVQCGTRFLSAVECQISDVYKQKVLSANDTD
                     TIVTGRRSGHPVRALKTPFTRKFAKMEQTS"
BASE COUNT         1572 a          715 c          909 g         1435 t
ORIGIN      
        1 atccaagtga ctgaatgtgg tagcaggagc agggtctgtt ttgtcatcag caggtacata
       61 aatagcttga actgatgtaa ttgatccatt tttagttgat gtgatacgct cttgcattct
      121 tcccatttct gtggcaaggg taggttggta tccaacagcc gaaggcatac gtcctagtaa
      181 agcagaaact tccgaacctg cttgtgtaaa cctgaatata ttatcaataa aaaacagaat
      241 atcatttcct ttaccatctt ttgcattggc atcacggaaa gattctgcaa cagtaagtcc
      301 agacaaggct acagatgcac gtgcgcctgg aggttcattc atctgtccga acaccaaggt
      361 gatttttgac tcttcaagtg cttttggatc aacctttgtt aaatcccaat cacctgcttc
      421 catgctcttt ttaaactctt caccataatt tattacgtta gattccaaca tctcacgtaa
      481 caaatcatta ccttcacgtg tacgttcacc aacgccggca aataccgaat atccaccata
      541 tcctttggca atattattaa tcaattccat gattaataca gttttaccaa caccggcacc
      601 accaaacaaa ccgattttac caccttttaa ataaggttca agtaagtcaa taaccttaat
      661 acctgtgtat aagatttctt tagatgtagc taagtcttca aatttaggtg gctcacgatg
      721 aatagggtat tcatttgaac ggtcaatgga accaataccg tcaatggcat cacctgtaac
      781 gtttagcaaa cgacctttaa tctgattgcc gataggcatt gagataggtg cgcccgttgg
      841 agttacttcc attccgcgta ccaagccatc tgtagaatcc atagcaattg cacgaactga
      901 gttctcgccg atatgctgtt ggcactctaa gattagtttt aaaccatttg atcttgttat
      961 ttgaagagca tcatgaattt ttggtagatt atctcctcct tcttcaaaat acacgtccac
     1021 aaccggacct ataatttgtg tgattgagcc attgatttga gccatagtta ctttctaaat
     1081 atagcttgct tccacaagct gtttttaacg gcttcaaagt tatctttttt tttcttaaaa
     1141 caagaattta cttgcgtttt tttttgaaca gcccataaaa tgctgatatt tttaatataa
     1201 agatgatata attataacaa attaaaatat cgataatggc ttttatttaa tacccggaaa
     1261 ggaatcgact attttaactg taatgtcggg gaatgaattg acaagctgta cttttattgt
     1321 tgggaaagaa tctacaattt ttactttgcc gcattcatca gcaaaagaat ctacaattgt
     1381 tactttaatg tcagcaaaag aatctacaat tttaaccttg atgtcgggga atgattccac
     1441 aattttaact ttgccataca gcggaaaagt ctttccgtct tttgtgattg tgcaattgtc
     1501 cttatcgatg ctaactttag caaatattga aatagggagg cataatagca atatgagtag
     1561 cagttttttc atataaaatt gatttgcaac aagacaaaat aataactgaa tataaaggta
     1621 tcaaaaaaaa tcaatttatg aagtaaaatt aaatttgttg atttacttgg taattatcta
     1681 aagacttcgc tttttgctat tttttttatc ataaaatatg atttactttg tactatgtca
     1741 taagttgaca aaactacaat gttaatttgc aaaaacaaag ggagagagat acaagcatgc
     1801 ttttgaaaat atatttgaga actgaatgat tttataacga ttaggtaaca tttttaatat
     1861 caattactat gaaagacaaa ttaaaggatc ttttattcct gaaagcccta ttggcatcaa
     1921 acaaagagtt agtggagatg aagaacaaag tccgcaagca gttcggagaa ccgacaatag
     1981 aagaggagat agcaatcttg gagaaagaac tcaaggagga agggaagtag gggttaaggg
     2041 tgagtggtta tgggttatgg gtgaagggtg aagggtgaag ggcgtgtaat gtttaatgtg
     2101 taatgtgtag gggcgaattg cattcgccct tcaaattcac aatgcgataa atttcaacat
     2161 tggattatac acgattacac aattccacga ttacacacta cacgattaca cgattacacg
     2221 attacacact aatatatttt gtcaccttga gataaatacg tagttttgta tcacaaatac
     2281 gcaatatatc atgttttcta ttagaaaatc agcaataata ttaagttttt tagcgattat
     2341 aatatcggta aatgctcaaa catatacctc attaaataat gaggattttg caaagcaact
     2401 taagaataaa aaactgcagc ttgtagatgt gagaactcca agtgagtatg cggcagggca
     2461 tcttccaaag gccattaata tagattttaa gaatcaagat ttcgagaaaa atatattaaa
     2521 actaaataag aaaaagaaag tagcagttta ttgtagaagt ggagttagga gtaaaaaggc
     2581 tgctgaaatt ctatcaaaaa ataattatat agtaatagaa ttaaatacag gaatttccaa
     2641 gtggaatgga gatattgtta ctaaataggt aaaatcagta aaattttgcc aaatttcggt
     2701 ggggtaaatt gaaatatcac gaataaaaac aggtcaaaat aatcgtttta cccctttatt
     2761 taacagaatt cacccctata aaaagcattt ttatcccatt tgggagagat atagagtaca
     2821 aaaagggagg taatcccatt tgttttattg attaagttta gtgtgcttac tttgcagtca
     2881 aataaagtgc acactattat gaaaattatt ggaacaggaa aagctttgcc atctttatca
     2941 gtaacaaatg atatgttggc tgaatttttg gatacgagtg ataattggat tagcacacgt
     3001 acaggaatta aagagagaag attgctctca aaagacaatt tgttgggttt aacaactgac
     3061 gcttcgcgtt tagctcttga gaatgcagga attacagctg cagatttaga ttatattata
     3121 tgttctaatg ttgcaaataa atatgtaact ccggcattaa gttgtatatt gcaaggcgaa
     3181 attaacgcaa catgtccttg cattgatatt aatggggctt gtgcaggttt tatttatgca
     3241 ttagatattg cagaatcatt tttgcagacg aagccatatg ttaataatgt attagttgtt
     3301 tgtgcagaag aaccatcacg ttttacaaat tggaaagaac gtagtacaag tgttttattt
     3361 ggagatggtg cagctgcagt tattttatca aaaggagatg atttaaaatc ggcattgctt
     3421 actacaacaa gtaacgttga tgccttatat tatcaaagag atttggaaga ttcaccatat
     3481 gttgagaaga agatagatgc agctcctttg gtgatgaatg gaaaagatgt atttaagttg
     3541 gctgttgcat catcagtagc tgatattagt gaggtagtgg aaaaagccgg tttaactttt
     3601 aaagacattg attattttat gttgcatcag gcaaatatta gaataattga tgctatttgt
     3661 aatcaattgc aacaaccgga agaaaaattt ccacataata tagaaaggta tggaaacaca
     3721 tcgtcggcaa gtattccaat tttgttagac gaaatgaata gagacggacg tttaaaaaaa
     3781 ggaagtaaaa tagtattaag tgcttttgga gcaggattta caacaggtgc atgtgtaatt
     3841 gaatggtcaa aaagcagtaa cgaaaaaacc taaaaaaaat atagatcatg tttagaagta
     3901 gaatctgcga tttagtgggt attaaatacc cgatttttca agggggtatg gcatgggtgt
     3961 cagaatcaat attggcagcc tcagtttcaa atgccggagg tttaggtata atatcagcag
     4021 gtaatgctga cgcaaattat gtaagagaac aaattcgtaa atgcaaaaaa ttaaccgata
     4081 aaccatttgg tgtaaacgta atgttgttaa gtccttttgc agcagaagtt gcagaagtat
     4141 tggcacaaga aaaagttgca gtagttacaa caggtgcagg aaatccggga aaatacatgc
     4201 aaatgtggtt agatgccggc attaaagtta tacctgtagt accatcagta gctttggcac
     4261 aaaaagttga aagcattggt gcttgcgctg taattgcaga aggtggtgag tcgggtggac
     4321 atattggtga tttagcaaca attacacttg tacctcaagt gtgtcatgct gttaaagttc
     4381 cggttattgc tgcaggaggt attgctgatg gacgtggaat ggcagcagca tttatacttg
     4441 gagcagaagg tgttcaatgc ggaacacgtt tcttgtctgc agtagaatgt cagatcagtg
     4501 atgtttataa gcaaaaagta ttatcagcta atgatacaga tactattgta acaggtaggc
     4561 gttcaggtca tcctgtaaga gcattgaaaa ctccgtttac cagaaaattt gctaaaatgg
     4621 aacaaaccag t
//
LOCUS       DAOPID010000113         4607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_25174_length_4607_cov_3.799868, whole genome shotgun sequence.
ACCESSION   DAOPID010000113 DAOPID010000000
VERSION     DAOPID010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25174_length_4607_cov_3.799868"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..1646
                     /locus_tag="PK641_04710"
     CDS             81..1646
                     /locus_tag="PK641_04710"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HPG55682.1"
                     /translation="MNSPILFLHQNPFFRIVIALIIGIIVGIYFNIIGISFFSFIIAI
                     VLYTLKRFVFNKDEYKFRWMHGMAFVLVFVGIGNLVCILHKEAIQSVPEEQTFIATII
                     ETPTEKPRSVLITVDLQNKAINSYKSVLFLQKDSNALNLSEGDKIITHVRLKETDLSF
                     FNNKGIYATAYVTSTQWYLMKINTKFSLKNKANKASKALLGVFKDFINPNNFGLISAL
                     TFGDTSSLNSEIKQDFAKSGSSHVLSVSGLHIGIIYIAFSFIFSFLGKNKIGTIFKHI
                     CIILCIWIYAFLCGLPPSVVRSAAMLSLISGAYIFQKKSATYNAVFFSAFLMLLYEPM
                     YLYDIGFQLSYCAVLSILIFHKKIYKLIPLKNKILDNIWSLTSVSIAAQLGTFPISLY
                     YFHQFPTYFLLSNYIAIPCSTLLIYLSICLFFTSFIPIISGFIKTVINYIAEYMQDGI
                     SYICNFPYASINIEIELWQVYAIFGLILSTYMMLTKRRFCYVCSNLCIIIIFLTNGII
                     VDLCKLFTTFAEV"
     gene            1658..2680
                     /locus_tag="PK641_04715"
     CDS             1658..2680
                     /locus_tag="PK641_04715"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007567095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HPG55683.1"
                     /translation="MITKIIPEEKIISVLKSINKAAKIAIVTHKSPDGDAIGSSLALY
                     HFLFSQEKETTVIVPDSFPKYLDILPSSSEILVYEGNETQVIETIKQCDLVFCLDFNN
                     LKRIGKLNTHIAGSNAKKIMIDHHPDFIEFADITISHSEIASTSELIFRLICRMGYFS
                     EMTNETAECICAGMLTDTGGLAFNSNSPEIYTIFAELLRKGVDKDSLYRHLYNNYSEN
                     RMRLMGYVLAEKMKIYPEYHTAIVALDKSDLEKYDYQTGDTEGFVNMPLSIDSVYFSI
                     FIRQDHDRVKLSFRSQGDFPANKVAAEIFGGGGHLNAAGAESLQPIDETVKRIEEALP
                     RYFNDK"
     gene            2687..3904
                     /locus_tag="PK641_04720"
     CDS             2687..3904
                     /locus_tag="PK641_04720"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HPG55684.1"
                     /translation="MKYIIILGDGMADEPIDILGNKTPLQKARKPYIDLLAKKGSSGM
                     LATVPDGFKPGSEIANLEVLGYDVAKCFEGRGALEAASMGVAVLPGEMAMRCNIICIE
                     NKKIKNHSAGHISNEEAKELIDYLQKELGDDTFTFHNGISYRHLLIMKGGDKRISCTP
                     PHDVPGTPFADVMVKALVPEAEDTARKLNELTLKSQELLAKHPVNLKRIEMGKDPANS
                     IWVWSAGYKPQMKTMMESFGIKSGAVISAVDLIKGIGIYAGLKSIDVEGATGLYNTNY
                     EGKAQAAIDALKTHDFVFLHIEASDEAGHEGDVDLKIKTIEYLDNRIVRPIYEEVSKW
                     NEPVTIAVLPDHPTPCAIRTHTNAPVPFIIYRPNGIADAVENYDEFSAQKGSYGLLKG
                     TEFIETLFKKQNS"
     gene            3901..>4607
                     /locus_tag="PK641_04725"
     CDS             3901..>4607
                     /locus_tag="PK641_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPG55685.1"
                     /translation="MKYYSTNKLVNCVDLENAVVKGLAEDKGLFMPNNIKVLPASFYQ
                     NIDKMSFKEIAYNVADAFFGEDVDAESLKKIVYDTLNFDVPLVKVEDSIYSLELFHGP
                     TLAFKDVGARFMSRLLGYFIQKAGENKKINVLVATSGDTGSAVANGFLGVKGIHVYVL
                     YPKGKVSKIQESQFTTLGQNITALEIDGTFDDCQSLVKSAFMDADLNAKMKLTSANSI
                     NVARFLPQAFYYFYAYS"
BASE COUNT         1590 a          704 c          817 g         1496 t
ORIGIN      
        1 gctttggtag caggaaattt catatttaaa tcggaagatc caatagaaac tatcaaacaa
       61 ttaaaagcac tataacataa atgaattctc ccatcttatt cttacatcaa aatccttttt
      121 ttcgaattgt tattgctctg attataggta tcatagtcgg aatatacttt aacattattg
      181 ggataagttt tttttctttt attattgcaa tcgttttata tacactaaaa cgattcgtat
      241 ttaataaaga cgaatataaa tttagatgga tgcatggtat ggcatttgtc ctagtttttg
      301 ttggtatagg caatcttgta tgcattttgc ataaagaggc aattcaaagc gttccggaag
      361 aacaaacatt tattgcaaca attattgaaa cgccaacaga aaaaccaaga agcgtactaa
      421 taactgttga tttacaaaac aaagccataa atagctacaa atctgtactt tttttacaaa
      481 aagacagcaa tgctttaaat ttatcagaag gagataagat aataactcat gtaagactaa
      541 aggaaactga tttatctttt ttcaataaca aaggaattta tgcaactgca tatgtaacat
      601 ctacgcagtg gtatcttatg aaaatcaaca caaagttttc tctaaaaaat aaagccaata
      661 aagcaagtaa agctttactt ggagtattta aggactttat aaatccaaat aatttcggtt
      721 taatttcagc tttaactttt ggcgacacat catctttaaa cagcgaaata aagcaagact
      781 tcgccaaatc aggtagctct catgtacttt ctgttagcgg attacatata ggtataatat
      841 atatagcctt ttcgttcata ttcagttttt taggaaagaa caagattggc actattttta
      901 aacacatttg tataatatta tgcatttgga tatatgcatt tctgtgcgga ttaccaccgt
      961 ctgtagtacg ttcggcagca atgctgagtc taatctcagg agcatatatt tttcaaaaaa
     1021 aatccgcaac ttacaatgcg gttttttttt cggcattttt aatgctgcta tacgagccta
     1081 tgtatctata tgatattgga tttcaattaa gttattgcgc tgttttatca attctaatat
     1141 ttcacaaaaa gatatataag ttaatcccac taaaaaacaa aatacttgat aatatatggt
     1201 ctttaaccag tgtatcaatt gcagctcaat taggtacatt tcctatttca ttatactatt
     1261 ttcatcaatt tccaacatat tttttattaa gcaattatat cgctatacca tgttctacat
     1321 tgcttatcta tttatctata tgcttattct ttactagttt tattccaata atatcaggtt
     1381 ttatcaaaac tgtaattaat tacatagctg aatacatgca agatggcatc agttatattt
     1441 gcaattttcc gtatgccagt ataaatattg aaattgaatt gtggcaagta tatgcaatat
     1501 tcggtttaat tctaagcact tatatgatgc ttacaaaaag gcgattttgc tatgtttgtt
     1561 ctaatttatg tataataatt attttcctga ccaacggaat catagttgat ttatgcaaac
     1621 ttttcactac ttttgctgaa gtttagactg cataaatatg attacaaaaa ttattcctga
     1681 agagaagatt atttcagttt taaaatcgat caacaaggcc gctaaaattg ctatcgtaac
     1741 acataaaagt ccggatggcg atgcaattgg atcgtcactt gctttgtatc attttttatt
     1801 ttcacaagag aaagaaacaa cggttattgt tcctgactct tttccaaaat atttagatat
     1861 tttgccaagc agttctgaaa ttctagtcta tgaaggtaat gaaacacaag tgattgaaac
     1921 tattaaacaa tgtgatcttg ttttttgttt agatttcaat aatttgaaga gaattggaaa
     1981 actaaataca catattgcgg gaagtaatgc aaaaaaaatc atgattgatc atcatcctga
     2041 ttttatagaa tttgcagata taacgatttc acattctgaa attgcatcca caagcgaact
     2101 tatcttcaga ttaatatgtc gaatgggata tttttcggaa atgacaaatg aaacggcaga
     2161 atgtatttgt gcgggtatgc tgactgacac aggtggatta gcatttaatt ctaatagccc
     2221 cgaaatatat actattttcg ctgaactatt aaggaaaggt gttgataaag attcgctata
     2281 cagacactta tataataatt attctgaaaa cagaatgcgt ttaatgggtt atgtattagc
     2341 agaaaaaatg aaaatatacc ctgaatatca tactgcaata gttgctttgg ataaatctga
     2401 tcttgaaaaa tatgattatc aaacaggaga tactgaaggt tttgtaaata tgccattatc
     2461 aattgatagc gtctattttt caatatttat aagacaagat cacgatcgtg ttaagctatc
     2521 gtttcgttcg caaggcgatt ttccggcaaa taaagttgct gccgaaatat ttggaggtgg
     2581 cggtcattta aatgcagcag gagcagaaag tctgcaacca attgatgaaa ctgtaaaaag
     2641 aatagaagag gcactccccc gctatttcaa tgataaatga acagatatga aatacattat
     2701 cattttagga gatggaatgg ctgatgagcc aatagatatt ttaggtaata aaacaccact
     2761 acaaaaagcc cggaagccat atatagacct tttagcaaaa aagggaagtt ccggaatgtt
     2821 agcaactgtt cctgatggtt ttaaacctgg cagtgaaata gccaacctgg aagttttagg
     2881 ttacgatgta gccaaatgct ttgaaggtag aggcgcatta gaagcagcta gtatgggtgt
     2941 tgctgtttta cccggtgaaa tggctatgcg ttgcaatatt atttgcattg aaaataaaaa
     3001 aatcaagaat cattctgcag gacacattag caatgaagaa gctaaagagt taattgatta
     3061 cttgcaaaaa gaattgggtg atgatacgtt tacattccac aatgggattt catacaggca
     3121 tttgcttatt atgaaaggtg gagataaaag gatttcatgt acaccacctc atgatgttcc
     3181 cggtactcca tttgctgatg ttatggttaa ggcattagta cctgaagcag aggacactgc
     3241 acgtaaactt aatgagttga cattaaagtc tcaggaattg ttggcaaagc atcctgtaaa
     3301 cttgaaacgt attgaaatgg gtaaagaccc tgcaaatagt atatgggtgt ggtctgcagg
     3361 atacaaaccc caaatgaaaa ctatgatgga gtcatttgga ataaaaagtg gcgcggtaat
     3421 atcggctgtt gatttgatta aaggcatagg aatttacgca ggcttaaaat caattgatgt
     3481 agaaggtgca accggtttgt ataatacaaa ctatgaaggt aaggcacaag ctgctattga
     3541 tgcacttaaa acgcatgatt ttgtatttct acatatagaa gccagcgacg aagcgggaca
     3601 tgagggagac gtcgatttaa agataaaaac aattgaatat ttagataatc gcattgtacg
     3661 tcctatttac gaagaagtat cgaaatggaa tgaacctgtg acaattgcag ttttacccga
     3721 tcaccctaca ccttgtgcta ttcgtactca tactaatgct cctgttccgt ttattattta
     3781 cagacctaac gggattgcag atgcagttga aaattatgac gaattttctg cacaaaaagg
     3841 aagctacgga ttattaaaag gaactgaatt tatcgaaaca ttattcaaaa aacaaaactc
     3901 atgaaatatt acagcacaaa taaattagtc aattgtgtag atcttgaaaa cgctgttgta
     3961 aagggtttag ctgaagataa aggtttgttt atgcccaaca atatcaaagt gctgcctgca
     4021 tcattttatc agaatattga taaaatgtct tttaaagaaa ttgcatataa tgttgcagac
     4081 gcatttttcg gcgaagatgt tgatgctgaa tctctaaaga aaatagtcta tgatacatta
     4141 aacttcgatg taccattagt taaggttgaa gattcgatat atagcttaga attgttccat
     4201 ggacctacat tagcatttaa agatgttggc gctagattca tgtcgcgttt acttggatat
     4261 tttattcaaa aagcaggcga aaataaaaaa ataaatgtac ttgtagccac atcaggagac
     4321 accggtagtg cagttgctaa cggattttta ggagttaaag gcatacatgt atatgttcta
     4381 tatccgaaag ggaaagttag taaaattcag gagagccaat ttactacatt aggacaaaat
     4441 attaccgcac tcgaaattga tggtactttt gacgattgtc aaagtttggt taagtcagct
     4501 tttatggatg ctgacttgaa tgcaaaaatg aaattgacat ctgctaactc tatcaatgtt
     4561 gctcgcttct tgccacaagc attttattat ttttatgcat attcaca
//
LOCUS       DAOPID010000114         4462 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_26349_length_4462_cov_3.370547, whole genome shotgun sequence.
ACCESSION   DAOPID010000114 DAOPID010000000
VERSION     DAOPID010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26349_length_4462_cov_3.370547"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1438)
                     /locus_tag="PK641_04730"
     CDS             complement(<1..1438)
                     /locus_tag="PK641_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55686.1"
                     /translation="MTLCLGNTNVNATKKDVKGGFIVIDGEKFYQITNYDAMQAFFIS
                     IASESNLWMYLSSTGGLTAGRINPDTALFPYYTDDKITEGFESTGSKTILHVKLAGKE
                     YLWEPFSNRNAGVYKIERRIAKSTIGNKILFIEQNFDLGLTFQYAWMNADAYGWIKKS
                     KLVNDSNDKIEVEIIDGVQNVLPSGVDQLTQNKLSTLVDAYKKTELVADASMALFRME
                     SILVDRAEPSESLRANVIWNYGLDKASYLLSSRQLDAFRAGLEIEAENEAKGVRGAFL
                     AHTSFTISSKTEKTWFFVAEVSQDAVQVRNCIAFLKNEKNIPLLLEQSIAKGTAALNA
                     IVGEVDGIQETADDHGMARHFANVLFNTMRGGFYCDNYNIFGAAFAKHVKIFNTKVAA
                     RNAEFLKSLPETLSYKDLESAVLKQKDKQLYRLFQEYLPLTFSRRHGDPSRPWNMFNI
                     KVNDENGNKLISYQGNWRDIFQNWEALSL"
     gene            complement(1475..2881)
                     /locus_tag="PK641_04735"
     CDS             complement(1475..2881)
                     /locus_tag="PK641_04735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPG55687.1"
                     /translation="MKLSIKEKVGYSLGDTASHFVWDLVGFWLLFFYTEVFGISPIVA
                     GWIMFAGSIWDAVVDPIVGIITDRTNTRWGKFRPYLLFGAIPYAILAVLAFTTPPFSP
                     TGMIVYAAITCLLLRTAYAFVNLPYSSMSAVMTNDTYERAGLNTYRFIAAYVGQFIVT
                     GAALYLVNYFGAMGNNEVSFSLLDSIAGKKEGIVIDQAQGYQYTVIAFAILSLLFFFI
                     AFKTTRERVPAPKKQDNSVSKDFKYLFKNRPWIVLTVVGVVSFVMFAMQNAAIAYYFK
                     YFIKNTEEVQLFNIVGTLALIVALPFSKPLAKRFGNKNVFITSSIVSGIFFAAIYAAG
                     SNITLIYIFNVLAKMAYAPAVPLLWTMIADAADFGEWHLGRRTTGLCFSAAVFAQKVG
                     WAIGAAAAGWILEMSNFVSNGDTQPDSAIFGIKLLVSVIPGVLYASCAIVMAFYNIDA
                     DTTTQMKIDLEAKRASEL"
     gene            complement(2931..3935)
                     /locus_tag="PK641_04740"
     CDS             complement(2931..3935)
                     /locus_tag="PK641_04740"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HPG55688.1"
                     /translation="MKTHLCLVGAMFITLCLNAQEYKLVWEDDFNANKIDSTYWNFED
                     NARGGGNGEMQYYAPKNASIETHPSGVSCLVLNAQKEDYKNRPATSARLNTQDKLSFC
                     YGKVEIRVCLPKTANGLWPAFWMLGDNLVKDMGNDDSVDKRLKQIKKEGRVVWPRCGE
                     IDILEMGHADGIKNGTQDRLFNGACHWGENFNNGKYPNIGMTKTTDESIQGDFHTFTL
                     IWDADSIRMYLDKDQNPECKPYFALGIKGKGKKNHPSRYFNKPFYLVLNLAVGGYFTG
                     LPAPEKYAAVITDDWKNFVEITNTALPKDGTPVKMYVDYIKIYQKGDKGESLNISGRK
                     "
     gene            complement(3985..>4462)
                     /locus_tag="PK641_04745"
     CDS             complement(3985..>4462)
                     /locus_tag="PK641_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HPG55689.1"
                     /translation="RCGEIDIVEMGNSGGFQTVDKSERFLNGALHWGYYVDGNYPNYG
                     KSSTYSYSVQDGNYHLFTLLWDENSVKMYIDLDKYPDAKAYYSMDITAPDPMPAEPTW
                     DAGEYFHKDFFILFNLAVGGHFPGITKIDAITALNEENNYEAKMYIDYVKVYQKAD"
BASE COUNT         1410 a          919 c          666 g         1467 t
ORIGIN      
        1 ataatgatag ggcttcccaa ttttggaaga tatcacgcca attgccttga tatgaaatta
       61 gtttattgcc gttttcatca ttaactttaa tgttaaacat gttccaagga cgacttggat
      121 ccccatgacg acgactaaat gtcaatggaa gatattcttg gaataaacgg tataattgtt
      181 tgtctttttg tttcagcact gccgattcaa gatctttgta tgataatgtt tcgggtaatg
      241 atttcaaaaa ttcagcattg cgagcagcta ctttagtatt aaatatcttt acgtgttttg
      301 caaaagcagc tccaaaaata ttataattat cgcaatagaa accaccacgc atagtattaa
      361 acaaaacatt tgcaaagtga cgtgccattc catggtcatc agctgtttct tgaataccat
      421 caacttctcc tacaattgca tttaaagctg ctgttccttt tgctatgctt tgttctaaca
      481 agagaggtat attcttttca tttttcaaaa atgcaatgca attacgaact tgtacagcat
      541 cttgcgaaac ttctgcaaca aagaaccatg ttttttcggt tttacttgat atagtaaacg
      601 aagtatgtgc aagaaatgct ccacgaaccc cttttgcctc attttcagct tcaatttcaa
      661 gacctgctct aaacgcatcc aattgacgag aactcaataa atatgatgct ttatccaaac
      721 cgtaattcca gataacatta gcacgtaaag actcacttgg ttctgctcta tcaaccaaaa
      781 tagactccat tctgaacaat gccattgaag catctgctac aagttctgtc tttttatatg
      841 catcaactaa ggttgataat ttgttttgtg taagttgatc tactcccgaa ggcaatacat
      901 tctgaacacc atctataatt tcaacttcaa ttttatcatt gctgtcatta acaagtttac
      961 tcttcttgat ccaaccataa gcatctgcat tcatccatgc atattgaaat gtcagaccta
     1021 aatcaaaatt ctgctcaata aacaatatct tgttacctat tgtgcttttt gctattcggc
     1081 gctcaatttt atatactcct gcattacgat ttgagaatgg ttcccataaa tattctttac
     1141 ctgctaactt tacatgtaag attgttttgc tgcctgtcga ttcaaaaccc tctgttattt
     1201 tatcatctgt gtagtatgga aataatgcag tatcagggtt gatacgacct gccgtaagac
     1261 cccctgtact tgacagatac atccataagt ttgattcgct ggctatgctg ataaaaaatg
     1321 cttgcatagc atcgtaattt gttatttgat agaatttttc tccatcaatt acaataaaac
     1381 caccttttac gtctttttta gtggcattta catttgtatt tcctaagcaa agagtcattt
     1441 taataaaact tatcagttaa tgtttagtat ttacttaaag ttcgctggca cgtttagcct
     1501 ctaaatcaat tttcatttga gttgttgtat ctgcatctat attgtaaaat gccattacta
     1561 ttgcacatga ggcatataat acacccggaa taacagaaac aagtaactta ataccaaaaa
     1621 ttgcactatc aggttgagta tcgccatttg atacaaaatt tgacatttct aagatccagc
     1681 cggctgcagc tgcaccaatt gcccaaccta ctttttgtgc gaatactgct gcagaaaagc
     1741 ataaacctgt tgttctacga cctaaatgcc actcgccgaa atctgcagca tcagcaatca
     1801 tagtccataa caatggaact gcaggtgcgt atgccatctt agctaacaca ttaaatatat
     1861 aaataagtgt aatattgctg ccggctgcat atatggcagc aaagaaaata cctgaaacaa
     1921 tagaactggt aataaataca ttcttattgc caaaacgctt tgctaaaggt tttgagaatg
     1981 gtaaagcaac aattaatgct aatgtaccta caatattaaa gagttgtact tcttcggtat
     2041 tcttaataaa atatttaaaa taatacgcaa tcgcagcatt ctgcattgca aacattacga
     2101 atgacactac acctacaaca gtcaacacaa tccaaggtct atttttaaat agatatttaa
     2161 aatctttaga tactgaatta tcttgctttt taggtgccgg aacacgttcg cgagttgttt
     2221 taaatgcgat aaagaagaat aataaactca atattgcaaa agctattacc gtgtattggt
     2281 aaccttgagc ctgatcaata acaattcctt ctttcttacc tgctatactg tctaataacg
     2341 aaaatgatac ttcgttattt cccatagcac caaaataatt taccaaatat aaagctgcac
     2401 cggttacaat aaattgtcca acataagctg caataaaacg ataggtattt aatcctgctc
     2461 tctcgtaagt atcgtttgtc ataacggcac tcattgaaga ataaggcaaa tttacaaatg
     2521 cgtatgctgt acgcaacaac aaacaagtga tagctgcata cactatcata cctgtaggac
     2581 taaacggagg agtagtaaat gctaatacgg ctaaaatagc atacggtatc gctccgaata
     2641 ataaatacgg gcgaaattta ccccaacgag tatttgttct gtctgttata atgcctacga
     2701 ttggatcaac tactgcatcc catatacttc cggcaaacat aatccagcct gctacaatag
     2761 gtgaaatgcc gaatacttca gtgtaaaaga ataatagcca aaatccaacc aaatcccaca
     2821 cgaaatggga tgcggtatcg cctaaactgt aacccacctt ttctttgatg gataatttca
     2881 tgtaattcat gttttgtttt gtattgtatt aataagtatc tcaaaaataa ttactttctg
     2941 cctgatatat ttaatgactc acccttatca cctttctgat atatctttat atagtcaaca
     3001 tacatcttaa caggggttcc gtctttgggt aatgcagtat tcgttatttc tacaaaattt
     3061 ttccaatcat ctgtaataac agctgcatat ttttcgggag caggtaaacc tgtaaaataa
     3121 ccacctacag ccaagtttaa taccaaatag aacggtttat taaaatatct gctaggatga
     3181 tttttctttc ctttaccctt aatacccaat gcaaaatatg gcttgcattc aggattttga
     3241 tccttatcta aatacattct tattgaatcg gcatcccata ttaatgtaaa tgtgtggaaa
     3301 tcaccttgta ttgactcatc agttgtttta gtcataccaa tattcgggta tttaccatta
     3361 ttgaaatttt caccccagtg acaagctcca ttaaataaac gatcttgcgt tccgttttta
     3421 ataccatctg catgccccat ttccaatatg tcaatttcgc cacagcgagg ccaaactaca
     3481 cgaccttcct ttttaatttg tttaagcctt ttatctacag aatcatcgtt acccatgtct
     3541 ttaacaagat tatcaccaag catccaaaat gcaggccata aaccatttgc agtcttaggc
     3601 agacaaactc taatctcgac ctttccgtaa cagaacgaca atttgtcttg agtattcaat
     3661 cttgcagagg ttgcaggcct gtttttgtaa tcttctttct gtgcgttaag tactaaacag
     3721 ctaactcctg aaggatgtgt ttcaatactt gcattctttg gtgcataata ctgcatctct
     3781 ccattaccgc ctcctctagc gttgtcttca aaattccaat aggtagagtc tatcttgttt
     3841 gcgttaaaat catcttccca aactaattta tattcttggg catttagaca caaagtgata
     3901 aacatcgccc ctaccaaaca taagtgcgtc ttcatattat atagttgtat ttatttagat
     3961 ttacaatctc tatccgatat cagcctaatc tgccttctga tataccttta catagtcaat
     4021 atacatctta gcctcgtaat tattttcttc gttaagagct gttatagcat caatttttgt
     4081 tatacccggg aaatgtcctc caacggcaag attaaacaaa atgaaaaaat ccttgtgaaa
     4141 atattcaccg gcatcccaag taggttcagc tggcatcgga tccggagccg ttatatccat
     4201 tgaataatat gcttttgcat caggatattt atccaagtct atatacattt taactgaatt
     4261 ctcgtcccat aatagagtaa ataggtgata gttaccatct tgaacactat aagaataagt
     4321 agatgatttt ccgtaattcg gataattacc atcaacgtag tatccccagt ggagagctcc
     4381 gttcaagaat ctctcagatt tatctacagt ttgaaaacct ccgctattcc ccatctcaac
     4441 aatatcaatt tcaccacaac ga
//
LOCUS       DAOPID010000115         4460 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_26371_length_4460_cov_4.535301, whole genome shotgun sequence.
ACCESSION   DAOPID010000115 DAOPID010000000
VERSION     DAOPID010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26371_length_4460_cov_4.535301"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..3934
                     /locus_tag="PK641_04750"
     CDS             62..3934
                     /locus_tag="PK641_04750"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HPG55690.1"
                     /translation="MSQLSKIDSFQNSFRFNFSMPKSYIKGNDLACFAPILKGLRNPI
                     KTSAAINYSNKTLRLKDFSASYGNNINIHTSANISDPFNPNETFVYADISEVRIGRDE
                     LENLISDLTKEPFILPKEMQNLGDLLYKGNISGFYSNLVAYGRLITQVGVIKTDLMMS
                     YIPNNQNIKFKGKIGTQDFNLNRLLGKDTKLQHLSMNVEVDGEIPQNKKITGKVSGRI
                     NSFTYNGYDFNDITIDGNYSQENASADIKYGDENGNGNILIHSDIDFKDKIKKFNLFA
                     RVDSIKPNPLKLIDSYPTFKASLIAKSQLQFTTIDDILGYISIDSLSLFNDNESYNID
                     SLVVRATKENDIQRITISSEPIRAFLEGKYTFSTLGSNLQYLLTEELTTFKPIQLKRK
                     QPSNDFTFSFDISPISELTYVFEKDIYIEDTTFIRGKFSDFENSIELTAFTNNIFLGN
                     KSMDSVSLHIHNLQEKIQLRLETTLETKLDTTDLAFSARLDNNDLDINFEWLNSVEKD
                     FSGKLGLDVLFKHPKTEDALLNLECTLHPSEMILSDSLWRIKEGKILYDEQSLIVNNI
                     DFGSSNQYLRINGAAHKEDNSKIIQVDLKDIDLSYISEVFYMPDIKLLGIVSGSVSIS
                     EALKKPILNADVTAKQFGLNNFPLGDVSAKASFNYEKEQIDLYGEVLNSFGDTSYVHG
                     FVAPVRQEMLLDIDINNLKLNFIKPYLVSFANDMAGTARGKLYVGGGFKSIELWGDAY
                     VQDAMLSIDFLKTKFYFSDSIHIRKDRFILKDITMYDEKGNTAYVNGLVTHKLFNDFK
                     YKIELGVENALILNTTEKDMPDFYGTIYATGGAVINGDMNKVDIAVKAKPEKNSYFAV
                     PVNSYTTAADNKFITFVNKNDNKKRSAEERRKRRRMNIAPTTKINVDIAIEATPDVEA
                     QIIFDSHTGDIIKARGQGNLKVNIDNNANVKLFGKYDITKGEYNFSMQGAIRKKFEVG
                     ENSSISFDGNPMNGIMDINAMYQTSASLNDLLEESMLTDIKNRTVKVNCIANISGQIL
                     EPRVKFDINLPNSDDEVQRRVKSTINTEELMSQQMVFLLLMGKFYNPQVTKANSGNAL
                     AASFATSTLSSQLNYWISQISDDVNLGLNYQEDTKDATNNRSFEVNISTNLFDNRLLI
                     NGNVGYRTQYGSDDFIGDFDVEYKLNKSGRVRLKAYNHTNDKIYSTALYTQGLGIMYR
                     EDFDTWNNLFKYYKDVFRKKTPEEKAVEKQRKEKEKLIKAKEKANRKALREDRKRRHK
                     IFVEEEKQRKQQEKEKRAAEKAARKSSK"
     gene            complement(3955..>4460)
                     /locus_tag="PK641_04755"
     CDS             complement(3955..>4460)
                     /locus_tag="PK641_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HPG55691.1"
                     /translation="ARCVAVDASGCVEVLTSAAKYKGASVVEVLQNCIIFNNGTYDLV
                     ATAQGRTKNAIYLEHGKPMLFGENKEFGLMQEGFGLKVVKLGENGITEKDILVHDAHC
                     EDNILQLKLASMEGPDFPIALGVIRSVEAPTYNESVYSQIEDVKGKKHYSNFNELLLT
                     NDTWEVK"
BASE COUNT         1702 a          673 c          722 g         1363 t
ORIGIN      
        1 taatagttta actatctaat cagatatcag attagatagt ttaactatta gttatgacag
       61 tttgtctcaa ctaagtaaaa ttgactcatt ccaaaatagt tttaggttta atttttcaat
      121 gccaaaatca tatatcaaag gaaatgattt ggcttgtttt gctcctattt taaagggttt
      181 aagaaatcct ataaaaacat ctgcagctat aaattattct aataaaactc taagattaaa
      241 ggatttttcg gcttcttatg gaaataatat aaatatacat acttcagcta atatatcgga
      301 tccttttaat ccaaacgaaa catttgtgta tgcagacatt agtgaagtaa ggattggacg
      361 agatgaatta gaaaatctca tatccgattt aacaaaagaa ccatttatat tgccaaaaga
      421 aatgcaaaat cttggtgatt tgctttataa gggtaatata tccggatttt attcaaatct
      481 cgttgcatac ggccgattaa taacacaagt cggagtcata aaaacagatt tgatgatgag
      541 ttatattcct aataatcaaa atattaagtt taaaggaaag attggaactc aagacttcaa
      601 tttaaacaga ttactaggta aagacacaaa gctacaacat ctttcaatga atgttgaagt
      661 tgatggagaa atacctcaaa ataaaaaaat tacaggaaaa gtcagtggac gaataaattc
      721 atttacatac aatggatatg atttcaatga catcacaata gatggtaatt acagccaaga
      781 aaatgcatcc gcagatatta agtatggtga tgaaaatggg aatggaaata ttttaataca
      841 ttcagatatt gattttaagg ataaaattaa aaaattcaat ttatttgcca gagttgactc
      901 tatcaaacca aatcctctga aattgatcga ttcctatcca acattcaagg cttcattaat
      961 agcaaaatct caacttcaat ttacaacaat agatgatata ttgggatata taagtattga
     1021 ttctttgtca ttgtttaacg ataatgaatc atacaatata gatagtctcg ttgtaagagc
     1081 aacaaaagaa aacgatattc aacgcatcac aatcagttct gaacctataa gagcattttt
     1141 ggaaggtaag tatacatttt caacattagg atctaattta cagtatttac taacggaaga
     1201 attaacaaca tttaaaccga tacaattaaa acgcaaacaa ccatcaaacg attttacatt
     1261 tagttttgac atatccccca tttcagaatt aacttatgta tttgaaaaag acatttatat
     1321 tgaagatacg acttttataa gaggtaaatt cagcgacttt gaaaactcaa tagaactaac
     1381 agcctttacg aataatatct ttttgggtaa taagagtatg gattctgttt cactacatat
     1441 acataacttg caagaaaaaa ttcaattacg cttagaaaca accttggaaa ctaaattaga
     1501 cactaccgac cttgcttttt ctgctcgttt ggacaataat gatttagaca ttaattttga
     1561 gtggcttaat tctgttgaaa aagatttctc cggaaaatta ggacttgatg tattgtttaa
     1621 acatccaaaa actgaagatg cactcttaaa tcttgaatgc acattacacc cttccgaaat
     1681 gattcttagc gattcgctat ggcgaataaa agaaggtaaa atcttgtatg atgaacaatc
     1741 tttgatagtt aacaatatag atttcggaag ttcaaatcag tatttacgaa ttaatggtgc
     1801 tgcacacaaa gaagacaata gtaaaattat acaagttgac cttaaagaca tagacttatc
     1861 atacataagt gaggtctttt atatgcctga cattaaacta ttaggtatag taagtggttc
     1921 tgtttcgatt agtgaagcac tcaaaaaacc aattttaaat gctgatgtaa ctgcaaaaca
     1981 attcggatta aataactttc ctttaggcga tgtttctgca aaggcgagtt ttaattacga
     2041 aaaagaacaa attgatttat atggcgaagt acttaacagt tttggtgaca cctcgtatgt
     2101 acatggattt gtagcacctg taagacaaga aatgttattg gatattgata taaacaatct
     2161 taaattaaac ttcataaaac catatttagt atcatttgct aatgatatgg ctggtactgc
     2221 acgcggaaaa ttatatgttg gcggaggatt taaaagcatt gaattatggg gagatgcata
     2281 cgtgcaagat gctatgctgt caattgattt cttgaaaaca aaattttatt tctctgattc
     2341 cattcacata cggaaggacc gttttatatt gaaagatatc accatgtatg atgaaaaagg
     2401 aaacactgca tacgtaaatg gacttgttac tcacaaatta tttaatgact tcaagtataa
     2461 aatagagctc ggagttgaaa atgcacttat attaaatacc acagaaaagg atatgcctga
     2521 tttttatggt acaatatacg caaccggtgg agctgtaata aatggcgaca tgaataaagt
     2581 tgacattgca gtaaaagcta aacctgaaaa gaatagctat tttgcagttc ctgttaatag
     2641 ttacacaacg gcagctgata ataaatttat tacattcgta aataagaatg acaacaaaaa
     2701 gagatctgca gaagaacgaa ggaaacgtag aagaatgaat attgctccta ccactaaaat
     2761 taatgtagat atagcaatcg aagcaactcc ggatgtagaa gcacaaataa tatttgatag
     2821 tcacaccggc gacataataa aagcgagagg acaaggaaac cttaaggtta atatagacaa
     2881 taatgcaaat gtaaaattgt ttggcaaata tgatattaca aaaggtgaat acaatttttc
     2941 tatgcaagga gctattcgta aaaaatttga agtaggtgaa aatagcagta tatcgtttga
     3001 tggaaatcca atgaatggta ttatggatat taatgcaatg tatcaaacat cagcatcatt
     3061 aaatgactta cttgaagaga gtatgttgac tgacattaaa aatagaactg taaaagtaaa
     3121 ttgtattgct aatatatcag gtcaaatatt agaaccgcgt gttaaatttg atattaattt
     3181 accaaactca gacgatgaag ttcagagaag agtaaaatct actattaata ctgaagaatt
     3241 gatgagtcag caaatggtat ttttattgct tatgggtaaa ttttataatc cacaagtaac
     3301 aaaagcaaat tcaggcaatg cactggctgc atcatttgcc acctctaccc tttcaagtca
     3361 attaaactat tggatttcac aaattagtga tgatgtgaat ttaggattaa actatcaaga
     3421 agataccaaa gatgctacta ataatagatc ttttgaagtc aatatatcaa ctaatttatt
     3481 cgataacaga ttgttgataa atggcaatgt cggatatcgt acacaatatg gttcagatga
     3541 ttttatcgga gactttgatg ttgaatacaa attaaacaaa tccggacgag taagactaaa
     3601 ggcttataat cacacaaatg ataagattta ttcaactgca ctttataccc aaggattagg
     3661 tattatgtat cgtgaagatt tcgatacatg gaataatctt tttaaatact ataaagatgt
     3721 attccgcaaa aaaactccgg aagaaaaagc tgttgaaaaa caacgtaaag aaaaagaaaa
     3781 gctaattaag gctaaagaaa aagctaacag aaaagcatta agagaagacc gaaaacgcag
     3841 acataagatt tttgtggaag aagaaaagca gcgcaagcag caggaaaaag aaaaacgagc
     3901 tgccgaaaaa gcagctcgta aatctagtaa atgatggaat caattattta ttaattattt
     3961 cacttcccat gtatcattag taagcaacaa ctcattgaag ttactataat gttttttgcc
     4021 cttaacatct tcaatttgag aataaactga ctcgttatat gtaggagctt caacactacg
     4081 aataacacct agtgcaattg gaaaatcagg accttccatt gaggctaatt ttagttgtaa
     4141 gatattatcc tcacaatgtg catcatgtac aagaatatct ttttctgtaa ttccgttttc
     4201 tcccaattta acgactttca atccaaaacc ttcttgcatt agaccaaatt ctttattctc
     4261 accaaacaac attggtttac catgctccaa ataaattgca ttttttgtac gaccttgagc
     4321 agttgcaact aaatcatacg ttccattatt gaaaataata cagttttgca aaacttcaac
     4381 aacggatgcg cctttatatt ttgctgctga agtcaatact tctacacaac cgcttgcatc
     4441 aactgcaacg catcttgcaa
//
LOCUS       DAOPID010000116         4456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_26418_length_4456_cov_3.970461, whole genome shotgun sequence.
ACCESSION   DAOPID010000116 DAOPID010000000
VERSION     DAOPID010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26418_length_4456_cov_3.970461"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PK641_04760"
     CDS             <1..483
                     /locus_tag="PK641_04760"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HPG55692.1"
                     /translation="GYAMIPIIERDIVDKRRWMPREEFIEMLALVQSVPGPISINTSI
                     YVGYKVLGWAGAIAAIFGIIIPSFVIILLIAIVFTDISSNPVIERVFKGIRPAVVALI
                     AAPIVRMAKAIGITWKTAWIPIAAALLIWLAGVSPVYIILATICISILWNMFKKPNNK
                     "
     gene            489..1013
                     /locus_tag="PK641_04765"
     CDS             489..1013
                     /locus_tag="PK641_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009038952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HPG55693.1"
                     /translation="MLYLILFWTFFKIGLFGFGGGYAIAALIQHEVVDVNAWLTVTDF
                     TNIMAISQITPGPIAINCATYVGYSATQSIIGSLIATIAVCLPSLIIMGIICKFLIKF
                     KSNKYVSYAMSGLKPIVLGLLASAALLLANAENFIDVYSYIIFGIVAIASLFKVNPIM
                     LLVLSGIAGFYIYA"
     gene            complement(1017..2165)
                     /locus_tag="PK641_04770"
     CDS             complement(1017..2165)
                     /locus_tag="PK641_04770"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPG55694.1"
                     /translation="MSLEEKFILLSTRLHISEDIKQELQNMSASALQWDKIFTIAGIH
                     RVDNLIYYALKQADLLNLIPNKYSQLFKLKYHSTAMANEKFMEDVNGIQSSLPQDKIV
                     LLKGSALLSNLYPNYGTRQMADIDILILKDRRDAVREQLLNSGWKSDFYAGYRSAIHR
                     ELAEKYSKTMYAVYKGRWERFLDIHWKFYIGKDDDKLAFEAMNTSLVVSGGYNVLSNE
                     MMLIHLCSNFADGFAAGEKLRTLCDIREFVRAKFIDWNKVEDVSKDTILYEKIALSLN
                     AVKWLYGDEVPKLYKRNYFDVIPPSLETLIARKKRKSKPTFWQTIKNSITAFQNPIKV
                     FVFLYKTIFPDVEWLRKMYPGKKSSHLRYWKYLFFRHILRADKRYRNF"
     gene            2390..>4456
                     /gene="nifJ"
                     /locus_tag="PK641_04775"
     CDS             2390..>4456
                     /gene="nifJ"
                     /locus_tag="PK641_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HPG55695.1"
                     /translation="MAKEKKFLTCDGNQAAAHISYMFSEVAAIYPITPSSTMAEYVDE
                     WAAAGRKNIFGETVLVQEMQSEGGAAGAVHGALQAGALTTTYTASQGLLLMIPNMYKI
                     AGELLPCVFHVSARTLASHALCIFGDHQDVMSCRQTGFAMLCEGSVQEVMDMSAVAHL
                     ATLKSRVPFVNFFDGFRTSHEIQKIESLDNDDLAPLIDQEALAEFRSRALNPMKPLMR
                     GMAENPDHFFQHRESSNPFYENVPAIVEEYMNELSKITGRSHGLFDYYGDKNAERVII
                     AMGSVTEAAREAIDYLQSKGEKVGMISVHLYRPFSAKHFIAALPKSAKRIAVLDRTKE
                     PGANGEPLYLDVKDVLYGQENPPLVVGGRYGLGSKDTTPAQILSVFENLSLPQPKNQF
                     TIGIVDDVTFTSLPVKEEIALGGAGMFEAKFYGLGADGTVGANKNSVKIIGDNTNKYC
                     QAYFSYDSKKSGGFTCSHLRFGDTPIRSTYLVNTPNFVACHVQAYLQMYDVTRGLQNN
                     GTFLLNTIWDGDTLIKNLPNNVKRYFAQKDIKVYYINATQIAQEIGLGNRTNTILQSA
                     FFRITAVIPVELAVEQMKKFIMKSYGKKGEDIVNKNYAAVDRGGEYKELVVDKAWGNL
                     KDDAVVENNDPDFINNIVRPINAQNGDLLPVSAFKGIEDGTWYPGTAKYEKRGVAAFV
                     PTWTAAN"
BASE COUNT         1434 a          822 c          756 g         1444 t
ORIGIN      
        1 ggttatgcta tgatacccat tattgaaagg gatatcgtag ataaaagacg atggatgcct
       61 agagaagaat ttatagaaat gttggccttg gtacaaagtg tacctggacc aatttctatt
      121 aatacgtcta tctatgttgg ctacaaagtt ttaggttggg caggtgcaat agcagcaatc
      181 tttggtatta tcataccatc attcgtaatt atacttctta tcgcaattgt atttaccgac
      241 atcagcagta atcctgttat agaaagagtt tttaagggta ttcgaccagc tgttgttgct
      301 ttaatagcag cacctatagt tcgtatggca aaggcaatag gcattacatg gaaaacagca
      361 tggattccta ttgcagcagc acttttgatt tggctggcag gagtgtctcc tgtgtacata
      421 atactagcaa ctatttgcat cagcatttta tggaatatgt tcaaaaaacc taataacaaa
      481 taatcaaaat gttgtattta attctttttt ggacgttttt taaaatcgga ttatttggat
      541 tcggaggtgg atatgctatt gccgctctaa ttcaacacga agttgtggat gtaaatgcat
      601 ggctcacggt tactgatttt acaaatatca tggctatatc tcagataaca cccggaccta
      661 ttgctatcaa ttgtgcaaca tacgtcggtt attctgcaac acaatcaatt ataggatcgc
      721 ttattgcaac aattgcagtc tgtttgccat cactcataat tatggggatt atctgtaaat
      781 ttttaatcaa gttcaaaagc aataaatacg taagttatgc aatgtcagga ctaaaaccta
      841 ttgttttggg attacttgca tctgctgctt tactactggc aaatgccgaa aattttatag
      901 acgtatacag ctacataatt ttcggaatag ttgcaattgc atcattattt aaagttaatc
      961 caataatgct tttagtactc agcggcattg ccggatttta catatatgct tgataatcaa
     1021 aaatttcgat agcgtttgtc tgcgcgcaat atatgtctaa aaaacaggta tttccaatat
     1081 cttaaatgtg atgatttttt acccggatac attttacgca gccattctac atccggaaat
     1141 attgttttat acaaaaacac aaacaccttt attggattct gaaatgcagt aatagaattc
     1201 tttattgttt gccaaaaagt gggttttgat tttctttttt tgcgtgcaat tagtgtttct
     1261 aaggagggtg gaatcacatc aaaataattc cgtttataca atttcggtac ctcatcacca
     1321 tacaaccatt taacagcgtt caaacttaag gctatttttt cgtataaaat agtgtcttta
     1381 gatacatcct caactttgtt ccaatcaata aattttgcac gcacaaactc acgaatatca
     1441 cataatgttc taagtttttc acctgcagca aacccatcag caaaatttga gcataaatgt
     1501 attagcatca tctcgttaga taaaacatta taccctcctg atacaactaa agatgtattc
     1561 attgcttcaa atgctaattt atcatcgtcc tttcctatat aaaatttcca atgtatgtct
     1621 aaaaatcgct cccaccttcc tttataaaca gcatacatag ttttgctgta tttttcagcc
     1681 aattccctat gtattgcaga cctatatcct gcataaaaat cgcttttcca tcctgagttt
     1741 aaaagttgtt ctctaactgc atctcgccta tccttcaaaa tcaatatatc aatatcagcc
     1801 atttgccttg tcccataatt aggatacaga ttagaaagca aagcacttcc ttttaaaaga
     1861 acaatcttat cttgtggcaa tgatgattga ataccattta catcttccat aaatttttcg
     1921 ttagccattg cagtagaatg atactttaat ttgaacaact gcgaatattt attcgggatc
     1981 aaattaagca aatctgcttg ttttaaagca taatagataa gattatctac cctatgaatg
     2041 ccggctattg taaatatttt atcccattgc aaagccgatg cagacatatt ttgcaattcc
     2101 tgttttatat cttcagaaat atgcaaacga gttgataata atataaattt ttcttccaac
     2161 gacataacaa aattgttttc agaaaacaaa attagtcatt aatttttaat cattttgtaa
     2221 taaacactaa acactacaac ataataatct attattatta catttcgtga ttaatcgttt
     2281 aatatatttt gtaatctcta cattgcaaaa taccattttt tgtagtatct ttgcggtctc
     2341 aataatgata aaaacaaaac actgaatatc ataattttaa attataaaaa tggcgaaaga
     2401 aaagaaattc ttaacatgtg atggaaatca ggctgctgca catatctcgt atatgttctc
     2461 agaagtagct gctatttatc ctatcacacc atcttccact atggcagaat acgtggatga
     2521 atgggctgcc gccggtcgta aaaacatctt cggcgaaaca gtattagtac aagagatgca
     2581 atctgaagga ggtgcagcag gtgctgttca tggtgcattg caagcaggtg cattaacaac
     2641 cacctatacc gcatcacaag gcttattgtt aatgatacca aacatgtaca aaatcgcagg
     2701 cgaattactt ccttgcgtat ttcatgtatc agctcgtaca ttagcaagtc acgcactatg
     2761 tatttttggt gatcatcaag atgtaatgtc ttgccgtcaa accggttttg caatgctttg
     2821 cgaaggttca gttcaagaag ttatggacat gtctgctgtt gcacaccttg caacattaaa
     2881 atcacgtgta cctttcgtta atttctttga cggattccgc acttcacacg aaatacaaaa
     2941 aatcgaatct ttagataatg acgacttggc tccacttatc gaccaagaag ctttagctga
     3001 attccgttct cgcgcattaa acccgatgaa accgcttatg cgtggtatgg cagagaatcc
     3061 tgaccacttc ttccaacaca gagaatccag caatccgttc tatgagaatg tacctgctat
     3121 tgttgaagaa tacatgaacg aattatctaa aattacaggt cgttctcacg gtttgtttga
     3181 ttattatgga gataaaaatg cagaacgcgt tattatcgca atgggttctg taacagaagc
     3241 tgcacgtgaa gctattgatt atttgcaatc taaaggcgaa aaagttggta tgattagcgt
     3301 acatctttat cgtccattct ctgcaaaaca ctttattgct gctttaccta aatcagctaa
     3361 acgtatcgct gttcttgacc gtacaaaaga accgggtgca aatggcgaac ctctatattt
     3421 agatgtaaaa gatgttcttt acggacaaga aaaccctcct cttgtagtag gtggacgtta
     3481 tggtttgggt tcaaaagaca ccactcctgc tcaaattctt tctgtatttg aaaacttatc
     3541 attaccacaa cctaaaaacc aatttacaat aggcatagtt gatgatgtaa ctttcacatc
     3601 acttcctgtt aaagaagaaa tcgctttagg tggtgcaggt atgttcgaag ctaaattcta
     3661 tggtttagga gctgatggta ctgttggtgc aaacaaaaac tctgtaaaaa tcattggtga
     3721 caatacaaac aaatattgcc aagcatattt ctcatacgat tcaaagaaat caggtggttt
     3781 cacttgctct catcttcgtt ttggtgatac ccctatccgt tctacatatt tggtaaatac
     3841 tccaaacttt gtagcatgcc acgtacaagc ataccttcaa atgtatgatg taacacgcgg
     3901 tttgcaaaat aatggtacat tccttttgaa tactatttgg gatggcgata cattgattaa
     3961 aaacttgcct aacaacgtaa aacgttattt tgcacaaaaa gatatcaagg tatattatat
     4021 caatgcaaca caaattgcac aagaaatcgg tttaggaaat cgtactaaca caattctaca
     4081 atcagcattt ttccgtatca cagctgttat tcctgttgaa ttagccgttg agcaaatgaa
     4141 gaaattcatt atgaaatcat acggtaaaaa aggtgaagat attgttaaca agaactatgc
     4201 tgctgttgat cgcggtggag aatataaaga attggtagtt gataaagcat ggggtaatct
     4261 aaaagatgat gccgttgttg aaaacaacga tccggacttt attaataata ttgttcgtcc
     4321 tataaatgcg caaaatggtg atttactacc tgtttcagca ttcaaaggca ttgaagatgg
     4381 tacatggtat cctggaacag caaaatatga aaaacgtggc gttgccgcat tcgttccaac
     4441 atggacagct gcaaat
//
LOCUS       DAOPID010000117         4428 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_26664_length_4428_cov_2.941688, whole genome shotgun sequence.
ACCESSION   DAOPID010000117 DAOPID010000000
VERSION     DAOPID010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26664_length_4428_cov_2.941688"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2010)
                     /locus_tag="PK641_04780"
     CDS             complement(<1..2010)
                     /locus_tag="PK641_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HPG55696.1"
                     /translation="MKKLQKIFICLLAVLLPSLSASALNKHILVGYWHNFNNGAANGL
                     KLSQVSDSWDVINLSFAEPTSATSGELVYNPTADNIYASADEFKNDVKAAQAKGKKVL
                     LSIGGANGQVRLETQTARDNFIRTAKNIITTYGLDGLDIDFEGHSLSFNAGDYNIANP
                     TTPVIVNLIYALNEICNFYGNDFLLTFAPETFFVQQGYSYYGSVCYGCDSRAGAYLPV
                     LYAMKDRLSWLQVQYYNSGTIPSPEGSNSCGSESFVVNLVKMLATGFSLADPQNLLSG
                     PKSFPALRQDQIVIGVPSTTGSAGSGVLTTNQYISIFNTLINKGYTNLRGYMTWSINW
                     DKHYNYEWSAPLRSYFDGLLGGTITTGGDEEEEEEENGGATGTADCSGIEQWNSSKAY
                     NVYGEYVGKEIAYEGNIYTVVTGTWSPAGVTPNSTTYTGLWTLVRACGSGGTNNDGGD
                     SDSGSNDNPGEITNDRVLTQCLKKHLVIGYWHNFKNGASNGLKLADVNTGYDFLNVSF
                     GETDATDRAVVTFVLDNSIYANDAAFINDIKSCQARGQKVNLSLGGQNGIISVSSEND
                     KNKFVNSVIAIIEKYGFDGLDIDFEGTSAGGTSSSFINPGTNAQLMISAIREICNHFG
                     DNFILTMAPETAYVQFGINQGSAPAYLALIYGLRDKLTVLHVQLYN"
     gene            complement(2031..>4428)
                     /locus_tag="PK641_04785"
     CDS             complement(2031..>4428)
                     /locus_tag="PK641_04785"
                     /inference="COORDINATES: protein motif:HMM:NF025563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HPG55697.1"
                     /translation="IKCPWGAIGVWPAFWMMGQKIANPGWPRCGEIDIMEQMCTGDPN
                     SWNTTLATYHWDGNGGTNSVNDHSMYGLTCNVGEQLGNQYRIYGVEWTPSQLKGYVCD
                     GNGNNYRQIVVMDIPNDASRGLDAFHQADNEFYMILNIALGGSYTGYAIDGGFSSATM
                     YVDWIRIYQDYAAYSGSKITNRSNLTTGGGTGDGGEQTVTETVAFKNAATTLEQSKSY
                     SFNVNYTVAEQRDLWVALYRTSNYDLIGESKIVVNKGSGTANLTINLNNVPDAGNDYI
                     LLTDIRPLNGAWTTRIKQEEKRNVTIQQSCKTTNKGVATFYVDADYSGNAVSLEEGTY
                     TKSQMAEYCINDNSISSIKVLPGYKVTTFIDDNFNGTSKSWTGNVSNTGSTWNDVISS
                     IKIEAQGVTGKSGTYKIKGRNSAMYWGLKGNGTGAGTMLVQYNDEGDEKYQRFTLVEI
                     SNGVYNIKANTSDLVLDIKNASIDNRADVQTWTANGSKCQQYILIKVDNGYYQFVARH
                     CGKVIEVPDNSSVADKELKLWDNNSQKCSHWLLESPSHETYDYWVIYRDDVSDMSSYL
                     DLRNNGFAEWEGTVSATKASMYESNNALAYNINAAKDSWFGLGVLNTTGTYYFADIAN
                     YGLHFAYKTNYNGELSVKLGGTNGEYAVAFTPNYDNKWHTKELKLSDFTSKGLALGSC
                     TNNLLFSIVSEKTTKTGAEINIDDIYYYKKSVLPIVFTETESNKTDNISCYQISSDII
                     RINGISEGTIIDIIDLNGINKIRVLAESETQYIYVSNLVNGLYIVRINDTVLKIIINN
                     "
BASE COUNT         1293 a          888 c          701 g         1546 t
ORIGIN      
        1 gttatataat tgaacgtgta aaacggttag tttatcacgt aagccataaa ttaaagcaag
       61 gtatgcaggt gcacttcctt gatttatacc gaactgaacg tatgcagttt caggtgccat
      121 tgtcaaaatg aaattatcac caaagtggtt gcatatttca cgaattgcag aaatcatcaa
      181 ctgagcattg gttcccggat taataaatga agaacttgtg cctccggcag atgttccttc
      241 aaagtctata tctaaaccat caaatccata tttttctata atagctatta cgctatttac
      301 gaatttgttt ttatcgtttt ctgagcttac agagatgatg ccattttgtc cgccaagtga
      361 aagattaact ttttgaccac gagcttgaca tgattttata tcgttaataa atgcagcgtc
      421 attagcataa attgaattgt cgagtacaaa agtaacaaca gctctgtctg ttgcatcagt
      481 ctcaccaaaa gaaacgttca agaaatcgta tccggtatta acatcagcta atttaagtcc
      541 gtttgatgct ccatttttga aattgtgcca atagcctatt acaagatgtt tctttaagca
      601 ttgtgtaaga actctgtcgt ttgttatctc acccgggtta tcattacttc cactatctga
      661 atcaccacca tcgttatttg tgccaccact gccacaagca cgaactaaag tccataaacc
      721 tgtgtatgtt gtcgagtttg gtgttacacc tgcaggagac catgtgcccg taacaactgt
      781 atatatattg ccttcgtaag caatttcttt gcctacatac tcgccgtata cattatatgc
      841 tttagagcta ttccattgtt cgattcctga acaatcagct gttcctgtag ctcctccgtt
      901 ttcttcctct tcctcttctt catctccacc tgtagttata gttccaccca acaaaccatc
      961 aaaataagag cgtaatgggg cagaccattc ataattgtaa tgtttatccc aatttattga
     1021 ccaagtcata taaccacgca aatttgtgta tcctttgttt atcaaagtat tgaaaatgct
     1081 tatgtattgg ttagttgtaa gaacaccgct acctgcagag cccgttgtag aaggaactcc
     1141 tattacaatt tgatcttgac gtagagcagg aaatgattta ggaccgctta gtagattctg
     1201 agggtctgca aggctgaatc cggtagctaa catttttacc aaattaacta caaaagattc
     1261 agaaccacag ctattagaac cttccggtga aggtatcgtt cctgaattat aatattgaac
     1321 ttgtaaccat gataaacggt ctttcatcgc atatagtaca gggaggtatg caccggctct
     1381 actatcgcat ccgtaacaaa cagatccgta atagctatat ccttgttgta caaaaaaagt
     1441 ttcaggtgca aatgttagca agaaatcatt gccataaaaa ttgcatattt catttaatgc
     1501 atatataaga tttacaatta caggtgtagt agggttagca atgttataat cgccggcgtt
     1561 aaatgacaat gaatgtccct caaagtcgat gtctaatcca tctaatccgt aagttgttat
     1621 aatatttttt gctgtacgaa taaagttatc gcgagccgtt tgtgtttcca aacgtacttg
     1681 tccgtttgca ccgccaatcg aaagtaaaac ctttttacct tttgcttgtg cagcctttac
     1741 atcattttta aattcatctg ctgatgcata aatattgtca gcagtagggt tatatacaag
     1801 ttctccactt gtagcagatg taggttcggc gaacgataag ttaattacgt cccacgaatc
     1861 gcttacttga gatagtttta ggccattagc tgcaccatta ttaaaattat gccaataacc
     1921 gactaaaatg tgtttattaa gagctgatgc tgataaactt ggcagcaaaa cagctaataa
     1981 acagatgaaa attttctgta attttttcat tgcttttttt gattaaaata ttagttatta
     2041 attattattt ttaaaactgt gtcatttatt cgaactatgt ataatccatt aacaagatta
     2101 cttacatata tatattgtgt ttcgctttct gccaaaactc ttattttatt aatgccattt
     2161 agatctatta tatctataat tgttccttca gatattccgt ttatgcggat aatgtctgac
     2221 gaaatctgat agcacgaaat attgtctgtt ttattagatt ctgtttctgt gaagacgata
     2281 ggtaaaacag attttttgta ataataaatg tcatcgatgt tgatttctgc tcctgtttta
     2341 gttgtttttt cgcttacaat agaaaacaac aagttgtttg tacatgaacc cagagcgagt
     2401 cctttagaag taaaatcgct tagtttaagc tcttttgtgt gccatttatt gtcataattt
     2461 ggtgtgaacg cgactgcata ttctccattg gtacctccta attttacaga aagctctcca
     2521 ttgtaattgg ttttatatgc aaaatgtaaa ccgtagttag caatatctgc aaaataatat
     2581 gtgcctgtag tattcaaaac tcctaagcca aaccaagaat cttttgctgc atttatgtta
     2641 tatgccaaag cattattact ttcgtacata cttgcttttg tggcacttac agttccttcc
     2701 cattctgcaa agccattatt tcgcaaatcc aaatacgaag acatatcaga aacatcatct
     2761 ctgtaaatta cccaataatc atatgtttcg tgagatgggg attccaataa ccaatgtgaa
     2821 catttttggc tgttattatc ccataatttt aattctttat cagcgaccga actgttatca
     2881 ggaacttcta tcactttacc acaatgtctt gcaacaaatt gataataacc attatcaact
     2941 ttgattaata tatattgttg acacttactt ccgtttgcag tccatgtttg tacatcagct
     3001 ctattatcga tagatgcatt tttaatatcc aaaactaaat cactagtatt ggcttttatg
     3061 ttataaacac cattacttat ttcaaccaaa gtgaatcgtt gatatttttc atcaccctcg
     3121 tcattatatt gtaccaacat tgttcctgcg cctgttccat taccctttaa accccagtac
     3181 atagcactat tacgtccttt tattttatac gttcctgatt tacctgtaac tccttgtgcc
     3241 tcaatcttta ttgatgagat tacatcgttc catgtgctac ctgtattact tacatttcca
     3301 gtccatgatt tagaagttcc attaaaattg tcatcaataa atgttgttac tttatatccc
     3361 ggtaatactt tgatcgatga tatgctattg tcattgatac aatattctgc catttgtgat
     3421 ttagtatatg ttccttcttc taaactgaca gcatttccgc tataatcagc atccacataa
     3481 aaagtagcaa ctcctttgtt tgtagttttg caactttgtt gaattgtaac gtttcgtttt
     3541 tcttcttgtt taatacgtgt agtccatgct ccatttaaag gtcttatatc tgtgagtagg
     3601 atatagtcat taccggcatc aggaacatta tttaaattaa tagtaagatt tgcagtaccg
     3661 ctgcctttgt ttacaacaat cttagattca cctattaaat cgtaatttga agttctatac
     3721 aaagcaaccc acaaatcgcg ttgctctgct actgtgtagt ttacattaaa gctatatgat
     3781 ttactttgct cgagagtagt tgctgcattt ttgaatgcga ctgtttctgt aacagtttgt
     3841 tctccaccgt ctccggtgcc tccacctgta gttaaattag atctattagt aattttcgac
     3901 cctgaataag cagcataatc ttgatatata cgaatccagt ctacatacat tgttgctgaa
     3961 ctgaaacctc catcaatcgc ataacctgta taactaccgc caagggcgat attcagtatc
     4021 atatagaact cattatcagc ttgatggaat gcatctaatc cgcgagaagc atcatttggt
     4081 atatccataa caacaatttg tctgtagtta ttcccgtttc catcgcaaac atatcctttt
     4141 aattggcttg gagtccattc aaccccatat atacggtact gattacctaa ttgttctcca
     4201 acattgcaag ttaatccata catagaatgg tcattgacac tatttgttcc gccatttcca
     4261 tcccaatgat atgttgcaag tgttgtgttc caactatttg gatcacctgt gcacatttgc
     4321 tccataatat caatttcgcc gcaacgtggc caccccgggt tggctatttt ttgtcccatc
     4381 atccagaaag ccggccatac gccaatcgca ccccatggac attttatg
//
LOCUS       DAOPID010000118         4422 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_26711_length_4422_cov_4.196474, whole genome shotgun sequence.
ACCESSION   DAOPID010000118 DAOPID010000000
VERSION     DAOPID010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26711_length_4422_cov_4.196474"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PK641_04790"
     CDS             complement(<1..277)
                     /locus_tag="PK641_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55698.1"
                     /translation="MKRISLLVCLMVFFASIFASKSVYYGRIDGKQLYQVIVVNSLES
                     GSPQMLTTVPVRPDNTFQIEIDAKQSDFYTLMFQGSGNLRRTQYLVVH"
     gene            complement(274..1494)
                     /locus_tag="PK641_04795"
     CDS             complement(274..1494)
                     /locus_tag="PK641_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008155440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HPG55699.1"
                     /translation="MLSVIEKIKSINIEDYNYPLTNERIAKYPLAKRDESKLLVNRNG
                     VFSTKIFKDLSNELPTKSLLVFNNTKVIQARMLFQKDTGAHIEIFCLEPLDPNDYQLV
                     FQKTSFCIWKCMVGNLKKWKTEILSKSCLIRGESVELYAEKLKTTGNTHEIKFNWRGE
                     DVTFADVLEAFGELPIPPYLNRATEESDKTTYQTVYSKIKGSVAAPTAGLHFTDSVLD
                     NLSKIGIEKCELTLHVGAGTFQPVKTEKIGGHQMHTEVIEVPKSAILQIKASLEQVIA
                     VGTTSVRTLESLYYIGLRLSSGNEDLFVSQWEPYMSKSDMPVEDALNAILNFMDLHGL
                     SVLNASTQIIIAPGYEFKIVKGLITNFHQPKSTLLLLLAAFIGDNWKQMYDYALSGGF
                     RFLSYGDSSLLLKV"
     gene            complement(1588..>4422)
                     /locus_tag="PK641_04800"
     CDS             complement(1588..>4422)
                     /locus_tag="PK641_04800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPG55700.1"
                     /translation="VGTDIDMATVLVQNRVNQATSSLPSDVTRLGVTTEKKSSSILML
                     IGMTSDNEELYDNVYLNNYATLNIVDELKRIPGVGSVSLFGADEYSMRLWLDPEVLRV
                     RGLNPSDIVAVIKEQNMQVSAGKVGELPSANKEAFQYTLDVKGRLVNAEEFGNIVVKT
                     LPGGKYLRVKDVAKIELGSKSYTIDSKLNHKTASSICIYQLPDANSLEVASAVKAKME
                     ELSQRLPEGVHSEVVLDTTKFIEVSIDEVYQTLFEAIILVLIVILLFLQDFRATLIPA
                     ITIPVSLVGTFAVMGLMGFSINTLTLFGLILAIGIVVDDAILVVENTFRHMELGAANA
                     KEATLKAMGEISGPIVGTVLVLLAVFVPTAFIGGITGEMYKQFAITIAVSTAFSGLNA
                     LTLSPALCALILKKKEKQSNFFVYRYFNAGFDKLHKGYSWVISTFLRKSVAVLLTFCA
                     LTAIMAFGFIKMPSAFIPAEDQGYFLIMAELQDGASLNETMEVMEQAEQILDSIPGVD
                     KHVTISGMSMADGAQISSKASMFVMLKDWEERKTDETSISAIIKRFNAEAHMKIQKAT
                     VYGFMMPAIQGLGTSGGYELYVQDKSNMGVVELQKVSEEFAEQANQEKELSGLRTTFK
                     ATTPQIFLNIDRDKVKMQKLAISDVFNALSAYLGSSYVNDFTLYGKVYQVRLQAESRN
                     RSSSNDIMRLSVRNSEDKMVPFATFASIEQKTGTEVINRYNMYQAAYMSGNAAEGYSS
                     GQAEDKVARLAKEKFGNTFGFEWTSMAFQEQSAGSSIILIFALALIVVLMVLAAQYES
                     VTSPMAVIMAVPIALLGVILGCIILGLPINIYTQIGMVLMIALSAKNAILIVEFARDY
                     RKEGKSIRDAAFDAGQIRLRPILMTSFAFILGVFPLVTASGAGAASRVSLGIAVFAGM
                     IMCTVVGTLFIPNYYQFWQKIQEKYFEKHK"
BASE COUNT         1407 a          907 c          649 g         1459 t
ORIGIN      
        1 gatgcacgac taaatattgt gttctacgca gattgcctga accttgaaac atcaaagtat
       61 aaaaatcaga ctgttttgca tctatctcta tttgaaatgt attatcaggc cgaacaggaa
      121 cagttgtcaa catttgtgga gaacctgatt ctaagctatt tacaactata acttgatata
      181 attgtttgcc atcaatacga ccataataaa ctgatttgct tgcaaatatt gaagcaaaaa
      241 acaccataag acatactaat aaactaattc ttttcatacc tttaataata atgatgaatc
      301 accataagac aagaaacgga atccgcctga aagtgcataa tcgtacattt gtttccaatt
      361 atcaccaata aaagctgcta ataataatag taatgtgctt ttaggttgat gaaaattcgt
      421 tatcaatcct tttactattt taaactcata tccgggagca attattatct gagtgctcgc
      481 gttcaatacg cttagaccat gtaaatccat aaagtttaat atggcattta aagcatcttc
      541 aaccggcata tctgatttag acatataagg ttcccactgc gacacaaata aatcttcatt
      601 gcctgatgac aagcgcaaac caatatagta caaactctcc aatgtacgta ccgatgtcgt
      661 tcctactgca ataacttgtt ctaaagatgc tttaatttgc agtattgcag atttaggaac
      721 ttcaataact tccgtatgca tctggtgccc acctattttc tctgttttaa cgggctgaaa
      781 agtgccggct cctacatgta aagtcaactc acatttttcg attcctatct tacttaaatt
      841 gtctaaaaca gaatctgtaa aatgcaaacc tgccgttgga gcggctacag atcccttaat
      901 tttagaatat accgtttgat atgtcgtttt atcactttct tctgttgctc tatttagata
      961 cggcgggatt ggtagttctc caaaggcttc caatacatct gcaaatgtaa catcttcgcc
     1021 cctccaatta aactttattt catgcgtatt gccggtagtt ttaagctttt cggcatacaa
     1081 ttcaacactt tcgccacgta tcaaacatga ttttgataaa atttctgttt tccatttttt
     1141 aagattacct accatacatt tccatataca aaaactagtt ttttgaaaaa ctagttggta
     1201 atcattggga tctaaaggtt ctaaacagaa aatttctata tgtgcaccag tatccttttg
     1261 aaaaagcata cgtgcttgaa tcaccttagt attattaaat accaataatg attttgtcgg
     1321 caattcgttt gatagatcct tgaatatttt agtactgaaa acaccattgc ggttcaccag
     1381 taatttagac tcatcgcgtt tcgctaatgg atactttgca atacgttcgt ttgtaagtgg
     1441 ataattataa tcctctatat taatactttt gatcttttct atcactgata gcatgatgct
     1501 tatgctgctt ttatatctta tttttatctt atcgattatg atctttttaa gattctaatt
     1561 agtcttattt atcataagat tttgaaacta tttatgcttc tcaaaatact tttcttggat
     1621 tttttgccag aattgataat aattaggtat aaataaagta ccaactacag tacacattat
     1681 catacctgca aacacagcta tacctaaaga cacacggctt gctgcacctg ctccgcttgc
     1741 tgttaccaat gggaatacac ctaatataaa tgcaaatgat gtcattaata taggtcttaa
     1801 acggatttga ccggcatcaa aagcagcatc tctaatagac ttaccttctt ttctgtaatc
     1861 acgagcaaat tctacaatca aaatagcatt ttttgcagat agcgcaatca tgagaaccat
     1921 accaatttga gtgtaaatat taattggcaa ccccaatatt atacaaccta aaataacacc
     1981 aagtaaggca attggaaccg ccataattac tgccatagga ctggttacgc tctcgtattg
     2041 agctgcaagt accattaaca caacaattaa agctaatgca aatatcagaa ttattgaaga
     2101 accggcagat tgttcttgga acgccattga agtccattca aagccgaatg tgtttccaaa
     2161 tttttctttt gctaaccggg ccactttatc ttctgcttga cctgaactat aaccctcagc
     2221 agcattcccg gacatatatg cagcttgata catgttataa cgatttataa cttcagttcc
     2281 cgttttttgt tcaattgatg caaatgttgc aaatggcacc attttatctt ctgagtttct
     2341 aacacttaag cgcataatgt catttgaact ggaacgattc cgagattctg cttgaagtct
     2401 tacttgatac acttttccgt atagtgtaaa gtcatttaca taagacgatc caagatatgc
     2461 agatagcgca ttaaaaacat cactgatagc caatttttgc atttttacct tatcccgatc
     2521 aatattcaag aatatttgtg gagttgttgc cttgaatgtt gtgcgcaaac cgctcaattc
     2581 tttttcttga ttagcttgtt cggcaaattc ttctgaaact ttttgtaatt cgacaactcc
     2641 catattagat ttatcttgca catacaattc gtaaccacct gaggtaccta aaccttggat
     2701 tgcaggcatc ataaatccat aaactgttgc tttttgaatc ttcatatgtg cttctgcatt
     2761 aaaacgctta ataattgcag atattgaagt ttcatcggtt tttctttctt cccaatcttt
     2821 tagcataacg aacattgatg ctttactaga tatctgagct ccatcagcca ttgacatacc
     2881 tgaaatagta acatgcttgt caactccggg tattgaatct aaaatttgct cggcttgctc
     2941 cataacttcc atggtttcat ttagagatgc tccatcttga agttctgcca ttattaagaa
     3001 atagccttga tcttctgcag gaataaaagc tgaaggcatc ttaataaatc cgaatgccat
     3061 tattgcagtt aatgcacaga aagtcaataa aacagctaca gattttctca aaaaagtgga
     3121 tattacccac gaatatccct tatgtaattt atcaaaacct gcattgaaat acctatatac
     3181 aaagaaatta ctttgttttt ctttcttttt caaaataagt gcacacaaag caggacttaa
     3241 tgtcaaagca ttcaaaccac taaatgccgt agaaactgca attgttatag caaactgttt
     3301 atacatttca cccgtgatac caccaataaa tgcagttggt acaaacacag ccaatagcac
     3361 taatactgtt ccgacaatag gacctgaaat ttctcccata gccttaagag tcgcttcctt
     3421 tgcattagct gcacccaatt ccatatgtct gaaagtattt tccacaacga gtatcgcatc
     3481 atcgaccact atacctatag ccaatatcaa accgaacaat gtcaaggtgt taatagaaaa
     3541 tcccataaga cccatcactg caaaagttcc taccaatgaa acaggtatgg taattgcagg
     3601 aatcaatgtt gccctaaaat cttgtaaaaa caatagaata acaatcagaa ctaatataat
     3661 tgcctcgaac aaagtttggt atacctcatc aattgaaact tctataaatt ttgtagtatc
     3721 taacacaact tcagaatgaa caccttcggg taagcgctga gacagttcct ccatctttgc
     3781 ctttactgca cttgcaactt ccaaagaatt tgcatccggt aattgatata tacaaataga
     3841 acttgctgtt ttgtgattta acttactgtc tatagtgtat gatttactgc ctaattcaat
     3901 cttagcaaca tctttgacac gtaagtattt acctccgggc aaagttttta ctactatatt
     3961 accgaattct tcagcattta ctaatcggcc ttttacatct aaagtatact gaaatgcttc
     4021 tttgttagca gatggcaact ctcctacttt acctgcagaa acctgcatat tttgctcttt
     4081 aataacagca acaatatctg atggatttaa tcctctgaca cgtaaaactt ccggatcgag
     4141 ccacaaacgc atactatatt catctgcacc aaaaaggctt acagaaccga ctcctggtat
     4201 gcgcttaagt tcatcaacga tatttaaagt agcgtagtta tttaaataca cattatcgta
     4261 caattcttca ttatctgaag tcattccgat aagcatcaat atactacttg attttttctc
     4321 ggtagtcaca cctaaacgag ttacatcact tggcaaactt gaagttgctt gattaacacg
     4381 gttttgaacc aaaactgttg ccatatcgat atctgttcct ac
//
LOCUS       DAOPID010000119         4400 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_26887_length_4400_cov_4.915765, whole genome shotgun sequence.
ACCESSION   DAOPID010000119 DAOPID010000000
VERSION     DAOPID010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26887_length_4400_cov_4.915765"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PK641_04805"
     CDS             <1..514
                     /locus_tag="PK641_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55701.1"
                     /translation="AHAPLKEVVKETKLGYKTYMHGYRDTYNHREANDFKWNLVGQKP
                     NKQNRNDYANMYAFWQGLGKLRNSDYGKVFRVSEAVPEGYYTWILPKNESMLGYLVNE
                     KVLVLINASEKANSFDSVKLPAGKWRLVGTTEEVNLKGVKSSNKTTKVKQGLNKVDME
                     PTSLYIWVKD"
     gene            607..1692
                     /gene="trpS"
                     /locus_tag="PK641_04810"
     CDS             607..1692
                     /gene="trpS"
                     /locus_tag="PK641_04810"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPG55702.1"
                     /translation="MDKIILTGDRPTGKLHIGHYVGSLRRRVELQNSGEYKNIFIMIA
                     DAQALTDNADNPEKVRQNIIEVALDYLACGLDPEKSTLLIQSQIPELCELSFYYMNLV
                     TVSRLQRNPTVKTEIKMRNFEANIPVGFFTYPISQAADISAFKATTVPVGEDQSPMIE
                     QTAEIVRRFNYLYGETLVEPKVLLPTNQACLRLPGIDGKAKMSKSLGNCIYLSEEADE
                     VAKKVRIMYTDPNHIKLEDPGKIEGNVVFTYLDAFCKPEQFARYLPEYANLDELKAHY
                     RRGGLGDVKVKKFLTAVLEEELAPIRARRKEYQKDIPYVYEVLKKGSEAAREVAIKTL
                     SEVRSAMKINYFDDIELINSQVERSSK"
     gene            1739..2014
                     /locus_tag="PK641_04815"
     CDS             1739..2014
                     /locus_tag="PK641_04815"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG55703.1"
                     /translation="MENIKNKIDDLMRQEKYDDALASINAYLAENNNSDEAWFLAGNV
                     HRKMENWQGAMNAYIKAMDINPDSPAKAAYGMIVKILDFYDKNRYNQ"
     gene            2058..2537
                     /locus_tag="PK641_04820"
     CDS             2058..2537
                     /locus_tag="PK641_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55704.1"
                     /translation="MKKLIVFVSILCALVSCVGPEGPRGPVGPKGSDAEGDQWIVDEF
                     TVNEGDWLLYGTEGQLGSYYYCDLNDQNLSEWIFDNGSVEVYLFDNSEERGWIQQPLP
                     VTRFKSTDEGFLYQEEYDFDYSVGKIRVYVTYNDFATANKPSAISFRSVKQWWLADN"
     gene            complement(2579..3232)
                     /locus_tag="PK641_04825"
     CDS             complement(2579..3232)
                     /locus_tag="PK641_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG55705.1"
                     /translation="MINATNITKSFGQLQVLKGIDLHIDKGEFVSIVGASGAGKTTLL
                     QILGTLDKSDTGAISIDNTKVNELKSDKLADFRNKHIGFIFQFHQLLPEFTALENVEI
                     PALIAKRSKQDAEKNAKELLNMLGLAERMDHKPNELSGGEKQRVAVARALINRPSLIL
                     ADEPSGSLDSKNKEELHKLLFKLRDEFKLTILVVTHDFELAHISDRIIEMKDGLIVK"
     gene            complement(3229..3942)
                     /locus_tag="PK641_04830"
     CDS             complement(3229..3942)
                     /locus_tag="PK641_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HPG55706.1"
                     /translation="MEWLERTKLLLGKSGTDSLCHSKIIVIGVGGVGAYAAEMLCRSG
                     IGDITLVDADTIKESNINRQLPATTKTLGLNKATVMAKRLQEINSDANITPIIKFIEA
                     DSIEALFADNKYDYVVDAIDSISPKVALITYCIKHQIPIISSMGAGGRIDPSKIKFAD
                     ISKTFECPLAKTMRDRLKKEGIYKGLPVVFSTEPVNRSSILEVFDERNKRSTLGTISY
                     LPAIFGCYLAAHVIKNLTK"
     gene            complement(3930..>4400)
                     /locus_tag="PK641_04835"
     CDS             complement(3930..>4400)
                     /locus_tag="PK641_04835"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPG55707.1"
                     /translation="VKVDGIHLHIPNKRFLKNGIEFIDSKQDQLLNMLERVKSRQKAN
                     ITKEEEFELQNIAKETFPKRVKELAIQYGFKYADVSIRKMKSRWGSCSLKGNIHLSSY
                     LAALPTACIDYVILHELCHTRHMNHGPLFWREVEKVCPDYKNTRKIMRQHKWNG"
BASE COUNT         1410 a          682 c          814 g         1494 t
ORIGIN      
        1 ggcacatgct ccacttaaag aagttgttaa agaaaccaag ttaggatata aaacctatat
       61 gcatggctat cgtgatacat ataatcatcg tgaagcaaat gatttcaaat ggaatttagt
      121 cggtcaaaaa ccaaacaaac aaaatcgtaa tgattatgca aatatgtatg ctttttggca
      181 aggacttggc aaattgcgca actcagatta tggtaaagta tttagagttt cagaggcagt
      241 acctgaagga tactatactt ggatacttcc aaaaaatgaa agtatgttag gttatttggt
      301 taacgaaaag gtattggtat taattaacgc aagtgaaaaa gcaaatagct ttgattctgt
      361 caagttaccg gcaggaaaat ggagattggt tggtactaca gaagaggtta atttaaaagg
      421 tgtaaaatct tcaaataaaa ctactaaagt aaaacaaggt cttaataaag tagatatgga
      481 gccgacttca ttatatattt gggtaaaaga ttaattctta aaatcaatat ttaatgcatg
      541 gggatgtgta cacatcccca tgttttttat acctttgtga aattttttta aagaacaaga
      601 caatttatgg ataagattat tttgacaggc gatagaccta caggcaagtt acacataggt
      661 cactatgtgg ggtctttacg tcgtcgtgtt gaattacaaa attctggcga atacaaaaat
      721 atttttataa tgatagctga tgctcaggca ttaacagata atgctgataa tcctgaaaaa
      781 gtgcgtcaaa atattatcga agtagcgtta gactatttgg catgtggttt agatcctgaa
      841 aaatctactc ttttaattca atcgcagata ccggaattat gtgagttgtc attttattac
      901 atgaatcttg ttacggtttc tcgtttacag cgcaatccta ctgtaaaaac agaaatcaaa
      961 atgagaaatt ttgaagcaaa tatacctgtc ggttttttta cttatccaat aagtcaagct
     1021 gctgatataa gcgcatttaa agccacaact gttccggtag gagaggatca gtctccaatg
     1081 atagagcaaa cagccgaaat agtacgcaga tttaattact tatacggtga aacgctggta
     1141 gaacctaaag tattattacc tactaatcag gcttgtttac gtttgccggg tattgacgga
     1201 aaagcaaaaa tgagtaagtc tttaggcaat tgtatatatc tttcagagga agctgacgaa
     1261 gtcgctaaaa aggtgagaat aatgtataca gatccaaacc atattaaatt ggaagatccg
     1321 ggaaaaatag aaggtaatgt tgtgtttaca tatttggatg ctttctgtaa acctgaacaa
     1381 tttgctcgtt atttacccga atatgctaat ttagatgaac ttaaagccca ttatcgtaga
     1441 ggcggcttag gtgatgttaa ggttaaaaaa ttcttaactg ctgtactaga agaagagtta
     1501 gctccaattc gcgcaagacg aaaagaatat caaaaggaca taccttatgt ttacgaagtt
     1561 ttgaagaagg gaagcgaagc ggctcgcgaa gttgcgataa aaacattatc ggaggttcgt
     1621 tctgcaatga aaattaatta ttttgatgat atagagttga ttaactctca agtagagagg
     1681 tcctcaaaat gaggtgtgga gaattatttt catggattct tatttctact aattttttat
     1741 ggaaaatatt aaaaataaaa ttgatgattt gatgcggcaa gaaaaatacg atgatgcact
     1801 tgcttcaata aatgcatact tggcagaaaa taataattct gatgaggcct ggtttttagc
     1861 cggcaatgta catagaaaaa tggaaaattg gcaaggagct atgaatgctt atataaaagc
     1921 aatggatatt aatcctgaca gtcctgccaa agcagcttat ggaatgatag ttaaaatatt
     1981 ggatttttac gacaaaaatc gttataatca atagatataa attttaaata ttcacttatt
     2041 aattaataat ttaaaaaatg aaaaaattaa ttgtttttgt atcgatttta tgtgctttgg
     2101 tatcgtgcgt aggcccggag ggtccacgag gcccggtagg cccaaaagga agtgacgcag
     2161 aaggagatca atggattgta gacgaattta cagtaaatga aggagattgg ttgttgtacg
     2221 gaacagaagg acaactaggt tcttattatt attgtgactt gaatgatcaa aatttgtcag
     2281 aatggatttt tgataatggt tctgttgaag tttatttgtt tgataactcg gaagagcgtg
     2341 gttggattca acaaccactg cccgtaactc gttttaaatc aacagatgaa ggttttttat
     2401 atcaagagga atatgatttt gattattctg taggtaagat tcgtgtatat gtaacttaca
     2461 acgattttgc aacagcaaac aaacctagtg ctataagttt ccgaagtgta aaacaatggt
     2521 ggctggcgga taattaataa ctatagaaat aaaaaatgct tccccaagga agcatttttt
     2581 attttactat taaaccatct ttcatttcaa ttattctgtc ggatatgtga gctagttcaa
     2641 aatcgtgagt aactactaat attgtaagtt taaactcatc tcgtaatttg aataataatt
     2701 tgtgtaattc ttctttgttt ttactatcca aactgcctga tggctcatca gccaatatta
     2761 atgaaggtct atttattaaa gctcttgcaa ctgcaactct ttgtttctct cctcctgaaa
     2821 gttcgttagg tttatgatcc atacgttctg ccaaacctaa catattgagt aattctttag
     2881 cgtttttttc agcatcttgt ttgcttcttt ttgcaattaa tgcaggaatt tcaacatttt
     2941 caagtgcagt aaattcaggt aaaagttgat gaaactgaaa aataaatcca atatgtttat
     3001 tcctaaaatc agcgagttta tcagatttta attcattcac ttttgtatta tctatcgaga
     3061 tagcgcctgt atcagatttg tcaagtgtgc ctaatatttg taatagtgtt gtttttccag
     3121 caccgctggc tcccacaata gaaacgaatt cacctttgtc tatgtgtaag tcaatgcctt
     3181 ttaaaacttg cagttgaccg aatgattttg tgatatttgt tgcgtttatc attttgttag
     3241 attttttata acatgtgctg ctaaatagca tccgaaaatt gccggaagat atgatattgt
     3301 acctaatgtt gaacgtttat tcctttcatc gaacacttct aaaatcgaac ttctatttac
     3361 aggctctgtg ctgaatacaa ccggtaatcc tttatatatt ccttcctttt ttagtctgtc
     3421 gcgcattgtc ttggcaagtg gacattcaaa ggttttcgaa atatcggcaa atttaatctt
     3481 agacgggtca attctacctc cggctcccat agaagatata ataggaattt gatgttttat
     3541 gcaatatgtg ataagggcaa ctttgggaga tatcgaatcg atggcatcaa ctacatagtc
     3601 atatttattg tcagcgaata atgcttcaat agagtctgct tcaataaatt taattattgg
     3661 tgtaatattg gcatcagaat taatttcttg taatcgttta gccatcactg tagctttatt
     3721 tagtccaaga gtttttgtcg tggcaggtag ctgtctatta atattgcttt cttttatagt
     3781 atcggcatca actaatgtaa tatcaccaat accacttcta caaagcattt cagcagcata
     3841 agctccaact ccgccaactc caattactat tatttttgaa tggcaaagcg aatcagtacc
     3901 tgatttacct aaaagtagtt ttgtgcgctc taaccattcc atttgtgttg tctcattatt
     3961 ttacgtgtgt tcttgtaatc agggcaaacc ttttcaactt ctctccaaaa taatgggcca
     4021 tgattcatat gtcgtgtgtg gcaaagttcg tgtagtatca cgtaatcgat gcatgcagtt
     4081 ggtaatgccg ccaagtatga tgatagatga atattccctt ttagcgagca acttccccat
     4141 cttgatttca ttttcctaat gcttacatct gcatatttga atccatattg tattgctagt
     4201 tctttaacac gttttggaaa tgtttcttta gcaatatttt gtaattcaaa ttcttcctct
     4261 tttgtgatat ttgctttttg tctactttta accctttcga gcatatttaa aagttggtct
     4321 tgcttgctat ctataaattc tattccgttt tttaaaaagc gtttatttgg aatgtgtaga
     4381 tgtataccgt caacttttac
//
LOCUS       DAOPID010000120         4395 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_26930_length_4395_cov_3.975346, whole genome shotgun sequence.
ACCESSION   DAOPID010000120 DAOPID010000000
VERSION     DAOPID010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26930_length_4395_cov_3.975346"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..105
                     /locus_tag="PK641_04840"
     CDS             <1..105
                     /locus_tag="PK641_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008824037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HPG55708.1"
                     /translation="AWLVIEHGKKTNFSSHEHFIEERNYGNVHFSFFK"
     gene            complement(84..1127)
                     /locus_tag="PK641_04845"
     CDS             complement(84..1127)
                     /locus_tag="PK641_04845"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HPG55709.1"
                     /translation="MNFLSKGALWFCLIFCASCVNHLYSQSFRVAFYNVENYFDVRND
                     SLKDDNAFLPEGDYHWTWAKYRKKTISIAKVLTSLGEWDGAAIIGLCEVENDSVLDAL
                     IYRSPLKSKKYKYIHYESPDQRGVDVAMLFDPSRFEAINSKALNVGLKGSHSFKTRDI
                     LYVKGIVEKIDTLHIFVCHAPSRRGGEEQSEWKRMQSAAVMKGAIDSIRSNKVNANIL
                     IMGDFNDQPSNKSIAEVLKALHVNKNDSIKSDCLYNLYAQYENTEQGTHKFNGVWGVL
                     DQIIVSGSLLQGLNMHTSLLDAHIYVNEFLVKEDAKNMGVKPNSTYVGPVYNGGISDH
                     LPIYTDFYLKKEK"
     gene            complement(1111..2577)
                     /locus_tag="PK641_04850"
     CDS             complement(1111..2577)
                     /locus_tag="PK641_04850"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HPG55710.1"
                     /translation="MKKKSEHWIKRVLHSFVVFCFCLPMLICGVSCEEDRINSDPSLK
                     LEFSVDTLSYDTIFTDLGSTTKQLKIYNRSANKLQISSIKLSEGLFKLNVDGVKSNNL
                     SDVIINAHDSMIVFVQVYINPKDTNIPFLVKDSIMCYTNGNEQKILLTAYGRRAIRWD
                     RHIVAQDTTLTSYMPFLISDTLFVPQGVTLTILEGSNLFFRKEAVLLVEGKLIAQGTF
                     ENPVIFRGDRSDYMNTIPPLSYDKASEQWGGIRFKNGSIDNVLMNTDVRNTTFGIIVD
                     STTCDNQVLLIENSKIRNSSGNLLTMNNAKLEVNNSLLYNAGAFILELNGGDYQFNHC
                     TIANYYLFSWGTRTQAIAVFKNSVGITPCAFNALFNNTIIYGGYNNELTYDRDSHGEA
                     PFNYLYKNCLLRLLTSNIDEHYNNCLFNTDPKFVYTERSSEEKQTDPYNYDFHIKSGS
                     AAIGKGNQETAIKFPIDLDGRSRLSDGSYDIGAYEFFE"
     gene            2758..3048
                     /locus_tag="PK641_04855"
     CDS             2758..3048
                     /locus_tag="PK641_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55711.1"
                     /translation="MASRRNLKKDIQYIYSELLSECFTLSMLFPDIKKEENEAIFNKI
                     LVNNNDFIKRVSKTDGKCNSKIVKAYYRKLVTELLTDASNISKELQQLHKNS"
     gene            3053..3604
                     /locus_tag="PK641_04860"
     CDS             3053..3604
                     /locus_tag="PK641_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005788935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPG55712.1"
                     /translation="MKKICSFILKILGWKTINSVPDIDKMVLCVAPHTTNWDFIIGKL
                     FYSSMGLKASFLIKDDWLRPPFGTWMKKRGAIGVNRKGKHSMTEQLTELFKTQSRLHL
                     AITPEGTRKANPNWKLGFYYIAKKANVPILLIALDYKYKEAKILGLFTPTENEKEDIK
                     AIKRYYKDIHAKYPKAFDLGNID"
     gene            3606..4394
                     /locus_tag="PK641_04865"
     CDS             3606..4394
                     /locus_tag="PK641_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HPG55713.1"
                     /translation="MEKQEELRVRVVQSDIIWEDKEANLNHYNDLLSKADDGKTDIIL
                     LPEMFSTGFSMNAKKLAENTDGPTMKLAQSWAKQFNAAIIGSFIAQEDGLFYNRGFAA
                     MPNGKTYFYDKRHLFIGGEKQIFTPGDTHLTIAYKGWNIALCICYDLRFPVWLRNKDC
                     AYDLMLCMAEWPSNRQYIFEHLLIARAIENQSYVCACNRVGVDGNLLSYIGGSVLIDY
                     KGKVKERCTDNVEETRTFTLLKYPLESHREKVPVWKDGDSFYWA"
BASE COUNT         1509 a          775 c          691 g         1420 t
ORIGIN      
        1 gcttggttgg ttatagaaca cggaaagaaa accaacttta gttcgcacga gcattttatc
       61 gaagaacgca actatggaaa cgttcatttt tctttcttca aatagaaatc agtatatata
      121 ggtaagtgat ctgatattcc tccattataa accggaccga cgtaagtgct attgggtttt
      181 actcccatat tttttgcatc ctcttttact aaaaattcat ttacatatat atgagcatcc
      241 aataagcttg tatgcatatt taatccttgc aataagcttc ctgatacaat tatttgatct
      301 aatactcccc aaacaccatt aaatttgtgc gtgccttgtt ctgtattctc atattgagca
      361 taaagattat acaagcaatc tgattttatc gaatcatttt tgtttacatg caaagctttt
      421 aatacttcag ctatactttt attagaaggt tgatcattaa aatctcccat tatcaaaatg
      481 tttgcattta ccttattaga tcgaatgcta tctatagctc ctttcatcac tgccgcagat
      541 tgcatgcgtt tccattctga ctgttcttca cctcctctgc gcgagggtgc atgacacaca
      601 aatatatgta aagtatcgat tttttctaca attcctttta catataatat atctctggtt
      661 ttaaatgaat ggctaccttt caaaccaaca ttcaatgctt tgctgttaat agcttcaaat
      721 ctgcttggat caaacaacat agcaacatct accccacgtt gatcaggcga ctcgtaatgt
      781 atatatttat atttttttga ttttaaggga gatctatata tgagggcatc taatacagaa
      841 tcgttttcaa cctcacacag acctattatg gcagcaccat cccattcacc taaagaagtt
      901 agtacttttg ctatagatat tgttttttta cgatactttg cccaagtcca atgataatca
      961 ccttcaggca aaaaagcatt atcgtccttt agcgaatcgt ttctaacatc gaagtaattt
     1021 tcaacattat aaaaagcgac tctaaaagat tgagaataaa gatgatttac acacgaagcg
     1081 caaaatatta aacaaaacca caatgcgcct ttactcaaaa aattcataag cacctatgtc
     1141 gtaagatcca tcgcttagac ggcttctacc atctaagtca ataggaaatt taattgccgt
     1201 ttcttgattt cctttgccta tagcagccga tccggatttt atatgaaagt cataattata
     1261 aggatcggtt tgtttctctt cactagagcg ttcggtatat acgaatttag gatccgtatt
     1321 aaataaacaa ttattataat gctcgtctat atttgatgtt aaaagacgga gcagacaatt
     1381 tttatataaa taattgaatg gtgcttcacc atggctatcc ctgtcataag taagttcatt
     1441 attataacct ccatatatta tcgtgttgtt aaacaatgca ttaaaagcac atggagtaat
     1501 tccaacagaa tttttaaata ctgctatggc ttgtgtacgc gttccccacg aaaataaata
     1561 atagttagct atagtacaat gattaaattg ataatcgccc ccatttaatt caagaataaa
     1621 tgcgccggca ttatataata acgaattatt aacttctaac ttagcattgt tcatggttag
     1681 caaattacct gatgaatttc ttattttgct gttttcaata agcaaaactt gattatcgca
     1741 agttgttgag tctacaataa taccaaaagt tgtattacgt acatccgtgt tcatcaatac
     1801 attgtctata cttccatttt taaaacgtat tccgccccat tgttctgatg ctttatcata
     1861 agatagcggt ggtatggtat tcatgtaatc gcttctatca cctctaaata taaccggatt
     1921 ttcaaatgtt ccttgcgcaa taagtttacc ctcaactaaa agcacagcct cttttctaaa
     1981 aaacaaattt gaaccttcta aaatcgtcaa ggtaactcct tgcggaacaa acaatgtatc
     2041 agatataaga aacggcatgt atgatgttaa tgttgtatct tgtgctacaa tatgtctatc
     2101 ccaacgtata gctcgtctac catacgctgt caacaagatt ttttgctcgt taccattagt
     2161 gtaacacatt attgaatcct ttactaaaaa tggtatatta gtgtcttttg gattgatgta
     2221 aacttgtaca aaaacaatca tgctatcatg agcatttata ataacatcac ttaagttgtt
     2281 agattttacg ccgtctacat ttaacttaaa taagccctct gaaagtttta tagaacttat
     2341 ttgtaattta ttagcagacc tattatatat ttttaattgc ttggttgtgg atcctaaatc
     2401 ggtaaaaatc gtatcgtaag acaatgtatc gacagaaaac tccaatttta aacttggatc
     2461 gctatttatt ctatcttctt cgcacgaaac accgcatatt agcattggta ggcaaaagca
     2521 aaatactaca aatgaatgta gtaccctctt tatccaatgt tcagacttct ttttcatggc
     2581 acaaagatac attttttttg cagaaaaatc ttttacaaac aatctcttta agcttgatca
     2641 atcaattaat acactactga taatgtgaaa taatgaatta tctccggaat tatattattg
     2701 cacatcatat tatctttttg taaatttgta gaattaaact cataatttca actcaaaatg
     2761 gcaagtagac gcaatttaaa gaaagatata cagtatatct actcagaatt attatcagaa
     2821 tgtttcacat tatcaatgtt attccctgat attaaaaaag aagagaacga agctatcttt
     2881 aataagatat tggtaaataa caatgatttt attaagcgag taagcaaaac tgatggcaaa
     2941 tgcaactcaa aaatagtaaa agcatattat cgcaaattag taacagagtt attaacagac
     3001 gctagcaaca ttagcaaaga attgcagcaa ttacataaaa actcctgata cagtgaaaaa
     3061 aatctgctca tttatattaa agattctcgg ttggaaaacc atcaatagtg ttcctgatat
     3121 agataaaatg gttctttgcg tagctccaca cacaacaaat tgggatttta taataggcaa
     3181 actattttac tcttcaatgg gtttaaaagc gtcttttctt ataaaagatg attggttacg
     3241 tcctcctttt ggaacatgga tgaaaaaacg tggagctatc ggagtaaata ggaaaggtaa
     3301 gcattcaatg acagagcaac taacagagct ctttaaaacg caatcccgtt tacatctggc
     3361 aataacaccg gaaggtacaa gaaaagctaa tccaaattgg aagttgggct tttactatat
     3421 agctaaaaaa gctaatgttc caatattatt aattgctctg gattataaat acaaagaagc
     3481 aaaaattctt ggattattta cacctaccga aaacgaaaaa gaggatatta aggcgattaa
     3541 gagatactac aaagatatac atgcaaaata tccaaaggcc tttgatttag gcaatattga
     3601 ttaacatgga aaagcaagaa gaattacggg ttcgtgttgt acaaagcgac attatatggg
     3661 aagacaaaga agctaactta aatcactata atgatttact aagcaaagct gatgatggta
     3721 aaaccgatat aatactgcta cctgaaatgt ttagcacagg attcagtatg aatgcaaaaa
     3781 aacttgcaga aaatactgat ggtcccacaa tgaaacttgc acaatcatgg gcaaagcaat
     3841 ttaatgcagc tataattggt agttttattg cacaagaaga tggtttgttt tacaacagag
     3901 gatttgccgc aatgccgaac ggcaaaacat atttttacga caaaagacac ttatttatag
     3961 gtggtgaaaa acaaatattt acaccgggag atactcatct tactatagca tataaaggtt
     4021 ggaatattgc cttgtgtatt tgttatgact tacgtttccc tgtatggtta cgaaataaag
     4081 attgtgcata cgatttgatg ttatgtatgg cagaatggcc atcgaacaga caatatattt
     4141 tcgagcattt actaattgct cgtgcaatag aaaatcaatc atatgtatgc gcatgcaacc
     4201 gtgtaggtgt cgatgggaac ctactgtcct atattggagg atctgtatta attgactata
     4261 aaggaaaagt gaaggaacgc tgcaccgaca atgtcgaaga aacacgaacc ttcactttat
     4321 taaaatatcc actcgaaagc caccgcgaaa aagttccggt atggaaagat ggtgactcct
     4381 tttattgggc ttaac
//
LOCUS       DAOPID010000121         4383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_27045_length_4383_cov_3.956100, whole genome shotgun sequence.
ACCESSION   DAOPID010000121 DAOPID010000000
VERSION     DAOPID010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27045_length_4383_cov_3.956100"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PK641_04870"
     CDS             complement(<1..312)
                     /locus_tag="PK641_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HPG55714.1"
                     /translation="MKPTLYVMAAGMGSRYGGLKQMDGLGPSGETIIDYSIYDAIKVG
                     FGKIVFVIRHDFEEDFRNIVVAKFASKIPVEIVFQDLTNLPKGFTVNPERVKPWGTNH
                     AV"
     gene            complement(312..2216)
                     /locus_tag="PK641_04875"
     CDS             complement(312..2216)
                     /locus_tag="PK641_04875"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HPG55715.1"
                     /translation="MKRISILCALVALACTSLFANPNIVPIGGSKYARLSEDKTFIST
                     GDYKTGNEDTLLVFSKMRVNPLDSVEAFLFSPDGNRILLRASFENYYVYKIESRRSDK
                     LSAIDTQSKPMFSPDGKKIAFVRGNNIYVKRLEYDTEVVVTEDGSESIYNGLRSPEFE
                     KAFGIDVTMEWSPDGMYLLYTKNQNVYMYSMQYKWNKLITLPDKDAYYITRIAWTSKP
                     ETFSIMYLNRAQTVLRMAKVDAASLVAKRVYEYVEDRYISPDVASFVMFFPENDNFVV
                     LEERDDYKNLYLYSSAGSYIKALTKANYDITKVYGYDAASQRIFYQSTENGPDNRGIY
                     AIGVQNAKKTALSVTTGTNDASFSADYKFYELRHSAINSPDKYSICDIKGAELRVLEQ
                     DTVSEILHKEMYDFCGMSGWILRKDTLKHPLVMWVTEAKNQWDMDFAYRLVNEGYTVA
                     AVNARGTIGYGESFKKAVYGDMFVIPAQDYALAARCLVEQGKADAANMFIIGSGLSGA
                     VVLAALSQTDSPFKGGIAIAPVSDLASYNVAEVERLMQAQGATLAYKTNSAIYNAANI
                     KGKLLLVHAMNDKSMPLDNTLRLTEALVDANVQFDMQLYPNRDENFTMWLTQNHVFGK
                     VMNFLKINTK"
     gene            2368..4299
                     /gene="dnaK"
                     /locus_tag="PK641_04880"
     CDS             2368..4299
                     /gene="dnaK"
                     /locus_tag="PK641_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPG55716.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGNEPTIIANSEGKRTTPSIVAFID
                     GGERKVGDPAKRQAITNPKKTIFSIKRFMGETFDQTQKEIQRVPYTVVKGENNTPRVD
                     IDGRLYTPQEISAMILQKMKKTAEDYLGSEVTEAVITVPAYFSDAQRQATKEAGEIAG
                     LKVKRIVNEPTAAALAYGLDKTQKDLKIAVFDLGGGTFDISILELGDGVFEVKSTNGN
                     THLGGDDFDHVIIDWLADEFKNDEGLDLRKDPMALQRLKEAAEKAKIELSSSNSTEIN
                     LPYIMPVNGIPKHLVKTLSRAKFEQLADKLIQATIEPCKKAMSDAGFTNSDINEVILV
                     GGSSRIPAIQAIVEKIFGKTPSKGVNPDEVVAVGAAIQGGVLTGEVKDVLLLDVTPLS
                     LGIETMGGVMTKLIDANTTIPTRKSETFSTASDNQPSVQINVLQGERPMARDNKQIGM
                     FNLDGIPAAPRGVPQIEVTFDIDANGILKVSAKDKATGKEQNIRIEASSGLSDDEIKR
                     MKAEAEANADADKKEKERIDKLNQADGMIFQTEKQLKDLGDKIPADKKAPIESALEKL
                     KEAHKNQNIDAIDASMKELNDAFHAASQDMYNAANAGGAQGGQQAGPDFNAEQGGQNA
                     GSSANNGDNVQDADFEEVK"
BASE COUNT         1413 a          844 c          776 g         1350 t
ORIGIN      
        1 tacagcatga tttgtgcccc acggttttac tctttcagga ttgacagtga aacccttagg
       61 caaattagtc aaatcttgaa aaactatttc taccgggatt tttgaagcaa attttgcaac
      121 tactatattt ctaaaatctt cttcaaaatc gtgtcgaata acaaaaacga tctttccgaa
      181 tcctacttta atagcatcat aaatagaata atcaataatg gtctctccgc ttgggcccag
      241 gccgtccatt tgttttagac ctccataacg actacccata ccggcagcca taacatacaa
      301 agttggtttc attattttgt gtttattttt aaaaagttca ttactttacc aaaaacatgg
      361 ttctgtgtca accacattgt aaaattctca tctctattag gatacaattg catgtcaaat
      421 tgcacattag catcaactaa agcctctgtc aatcgtagtg tgttatctaa tggcatagac
      481 ttatcattca ttgcatgaac taacaacaat tttcctttaa tatttgctgc attatatatt
      541 gcagagtttg tcttgtaagc tagtgttgct ccttgtgcct gcatcaaacg ttctacttct
      601 gctacattgt atgatgctaa atcgcttacg ggagcaattg cgattcctcc tttaaatgga
      661 gaatcagttt gtgacaatgc tgccaaaacc acagcacccg ataatccact gcctataata
      721 aacatatttg ctgcatctgc cttcccttgt tcaactaaac aacgagcagc aagggcataa
      781 tcttgagcag gtatcacaaa catgtcacca taaactgcct ttttaaaact ttcgccgtat
      841 cctattgtac ctctggcatt tacagcagca accgtatagc cttcatttac caaacggtat
      901 gcaaaatcca tatcccattg attttttgct tctgtaaccc acattaccaa aggatgcttt
      961 aaagtatctt ttctcaaaat ccaacccgac atgccacaaa aatcatacat ttctttatgc
     1021 aatatttctg acactgtatc ttgctccaga acacgcaatt cagcaccttt tatatcgcag
     1081 atcgaatatt tgtcaggcga attaatcgca ctatgtctca attcgtaaaa tttatagtcg
     1141 gctgaaaaag atgcgtcatt agttcctgtt gtaacagaca atgctgtttt tttagcattt
     1201 tgtaccccta ttgcatatat gccacgattg tcaggtccat tttctgttga ctgataaaag
     1261 attctttgtg atgccgcatc ataaccatat actttggtaa tatcataatt agcttttgtc
     1321 aatgctttaa tatatgatcc tgctgaagaa tataaataga gatttttgta atcgtctctt
     1381 tcttccaaaa ctacaaaatt atcattttcg ggaaagaaca ttacgaatga tgccacatca
     1441 ggacttatat atcgatcttc aacatattca tatacgcgtt ttgcaactaa tgatgctgca
     1501 tcaaccttag ccattctcaa aacagtctgt gccctattca gatacattat cgaaaaagtt
     1561 tcaggttttg atgtccaggc tatacgagtt atataatagg catctttatc aggcaatgta
     1621 atcagcttat tccatttata ttgcatgcta tacatataca cattttggtt ctttgtatat
     1681 agcagataca tgccatcagg cgaccattcc attgtaacat ctatcccaaa ggctttttca
     1741 aactcaggac tacgcaagcc attatatata gactcgcttc catcctcagt cacaactact
     1801 tctgtatcgt attcaagtct ttttacatag atattatttc ctctcacaaa agcaattttc
     1861 ttaccatccg gcgaaaacat aggctttgac tgagtatcta ttgcagaaag tttgtcagaa
     1921 cgacggcttt ctatcttata aacataataa ttttcgaatg atgctcgtaa taatattcta
     1981 ttaccatcag gactaaaaag gaatgcttcc acactatcta aaggattgac acgcattttt
     2041 gaaaacacta acaaagtatc ttcatttccg gttttataat cgccggtcga aataaatgtt
     2101 ttatcctccg acaaacgagc atatttactt ccgcctattg gcacaatatt tggatttgca
     2161 aataaagaag tgcacgctaa ggcaactaga gcacacaata tggatattct tttcatgttt
     2221 caatatttta tgcaaagata agatttctac ttcatagatg attaaataaa atgccacttt
     2281 gtcagtaata attactttgg cacaaatttt gtcttatgct tagtgtgatt tgaaaatcaa
     2341 aattaataac aaaataaatt tacaattatg ggaaaaatta ttggtatcga cttaggaacc
     2401 acgaattcat gcgtggcagt tatggaaggc aatgagccta ccataatcgc aaacagtgaa
     2461 ggcaaacgta caactccatc aatagttgca tttatcgatg gcggcgagcg taaagttggt
     2521 gatccagcaa aacgtcaggc tatcacaaac cctaaaaaga ctatattttc aatcaaacgt
     2581 ttcatgggtg aaacttttga tcaaacacaa aaagaaatac aacgtgtacc ttatacagtt
     2641 gtaaaaggcg aaaacaacac tccacgtgtt gatattgacg gtcgtttata cactccgcaa
     2701 gaaatttctg caatgattct tcaaaagatg aagaaaacag cagaagatta tcttggaagc
     2761 gaagttacag aagctgttat tacagtacct gcatacttta gcgatgcaca acgccaagct
     2821 actaaagaag ccggcgaaat tgcaggttta aaagttaaac gtattgtaaa cgaacctact
     2881 gcagctgctt tagcttatgg tttggacaag actcaaaaag atttgaaaat cgcagtattt
     2941 gaccttggtg gtggtacttt cgatatttct atacttgagt tgggtgacgg tgtatttgag
     3001 gttaagtcta caaatggtaa tactcacctt ggtggtgatg attttgacca tgttattata
     3061 gactggttgg ctgacgaatt taaaaatgat gaaggcttag atcttcgtaa agatcctatg
     3121 gctttgcaac gtttaaaaga ggctgctgaa aaagcaaaaa tcgaattgtc aagttcaaat
     3181 tcaacagaaa tcaacttgcc atatatcatg ccggttaatg gtatacccaa acacttggtt
     3241 aagacattaa gccgtgctaa gtttgagcaa ttagctgata aattgataca agctactatc
     3301 gaaccatgta aaaaggcaat gagtgatgcc ggttttacaa acagcgatat taacgaggtt
     3361 attttggttg gtggttcatc tcgtattcct gcaattcaag caattgtaga gaaaatattt
     3421 ggtaaaactc caagtaaagg tgttaaccct gacgaagttg tagctgttgg tgctgctatt
     3481 caaggtggtg ttttaacagg tgaagtaaaa gatgtattat tgttggatgt tactcctcta
     3541 tctttaggta ttgaaacaat gggtggcgtt atgacaaaat tgattgatgc caacactacc
     3601 atacctactc gtaaatcgga aactttctca actgccagcg ataatcaacc ttctgtacaa
     3661 atcaatgtat tgcaaggtga acgtccaatg gcacgcgaca acaaacaaat cggtatgttc
     3721 aacttagacg gaatacctgc tgcaccacgt ggcgttcctc aaattgaagt aacattcgat
     3781 attgatgcta atggtatctt gaaggtttct gcaaaggata aagcaactgg taaagagcaa
     3841 aatattcgta ttgaggcttc ttcgggctta agtgatgacg aaatcaagcg tatgaaagct
     3901 gaggctgaag ctaacgctga tgctgataag aaagagaaag agcgcattga caaattgaat
     3961 caggctgacg gaatgatttt ccaaactgaa aagcaattga aagatcttgg tgataagatt
     4021 cctgctgaca aaaaggctcc tatcgaatct gctttggaaa aattgaaaga ggctcacaaa
     4081 aatcaaaaca ttgatgctat tgatgcttca atgaaagagt tgaatgatgc tttccatgct
     4141 gctagccaag atatgtacaa tgctgctaat gcaggtggtg ctcaaggtgg acagcaagct
     4201 ggtcctgact tcaacgctga gcaaggtggt cagaacgcag gttcttcagc aaacaatggc
     4261 gacaatgtac aagatgctga ttttgaagaa gtaaagtaaa agccaaaaac aaggattaat
     4321 atccatactc aaaccgctgt aaacacttag tttacagcgg ttttttattt tattatttat
     4381 gct
//
LOCUS       DAOPID010000122         4361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_27243_length_4361_cov_3.274036, whole genome shotgun sequence.
ACCESSION   DAOPID010000122 DAOPID010000000
VERSION     DAOPID010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27243_length_4361_cov_3.274036"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1245
                     /locus_tag="PK641_04885"
     CDS             <1..1245
                     /locus_tag="PK641_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55717.1"
                     /translation="DVFYGTTSESNYHIGFGGCTPSAITASISGTDSAAFTLPGAQPY
                     EVSLTDGAADLEISFSPTKVGAHTATLTFSTMCGETTYTKTVSLSATGKNHSITPLIA
                     DTADVTEGVVTLSGYLKYAGCKTDIIDYGFYYCEGADCTPSSSSDSIRSSEHTPISSG
                     SSWSAQPEGLKDKTWYSYKSYAKSTADADPVLSEETGHFYVTCDYPMGDTIYYTINAA
                     LSEDIPCTLEFSTFAAALTHLKSMSEYLASSSGNITNKLLKHIVFEVEAGVYGEANSQ
                     IDLSDINKITDNGQTIECPTHSLIVRRKNATDRPTLNGLKLYESRHITIDGFNITRST
                     TTSNMSGATISIGRNSDSNNAVPGNMKYANVSIINNTITATGFCCVHVQGADTIYYEN
                     NELYADITATGDPANDRNWVLQ"
     gene            1251..4130
                     /locus_tag="PK641_04890"
     CDS             1251..4130
                     /locus_tag="PK641_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55718.1"
                     /translation="MNCKNVQILRNSFRGTHATNLFIQGSRNFLIMNNVFWNDNQYTE
                     NVAFVRPVYFGGDGENNALQNIGIYYNTFFLKDEGGSTDYKVNFIEFGGAAQTGNATY
                     YHSNLMGFQYNNCYSYSLKVSGRDTDPFLGKNMETDICPTFKDNNFWSKYDVAQSEQD
                     TSAFAFGCSPIFINVENAVCWTASNDPASLVIKTDALNFGVSVKTDMSGMNIANTLYN
                     DRIYPSNLADAIRPKTNSSEEEPNEYGILSNAPTIRMSMSSMVETISHDVDMTSYTLT
                     PSSNVNFTLSGTNADKFSINKSSTTTDATGTSKDTVTVTFDRPATTGTYNAFLNISPS
                     TVSDVDTIAIPLIGIYADGPVSGGWTLGAYQQHEGNEVDVIVWNGTSDSPNDWDNRGN
                     WVKMDGTRVTCVDKLSEKLKVIIPAPDSKVYPMPAGDTIKTYPNMPTNFNAENRSVKQ
                     HAEQVDAGAGIIETTKFFADSIYLEYGASLRGVEKLVRTVGEQKDTLYNVATTNFTAQ
                     RSAFKDSREWILVGTVIKPFTGNGYETRNIKSGDFYIANQTPHVYMQHIKCTDGVNVS
                     WGTPFSSLQVEVPTTTAFAIEIPDQYGQYKLSSSLFYKYYYPDESMLNDGTQPKSFKF
                     EGRFANDASLPTYSIVSGVNFLNNSYPANLDAYTLHTSESYPGTVLMYDYELKSFRTP
                     QSGEVIKPQNGFIFNATSTTSMTLGADLFVDGNTRYKRALDEKPNIYIRAINAGRSLG
                     SQIALVYDEFKEQTIDPNVDARKPFNKESSLSPELYIMAQDAAFASLNITNMETVIPL
                     GLRNASKKLMDVKFVLRAQEGFGSAVLVDRQEGKEYDLLTGEAYTITNIAAGTIEGRF
                     FLNLSGIEDPDPIDPENPPTDVEDTELVGESISIFAQDGSVVISSTEGVALQTVYVSD
                     MAGKTDTYKLSGSHYQKLDLPVAKGVYLISVVGDTMTKEQKVIIK"
BASE COUNT         1450 a          739 c          874 g         1298 t
ORIGIN      
        1 gatgtatttt acggaacaac atcagaaagc aattaccata ttggatttgg cggatgcacg
       61 ccttcagcaa taactgcttc tataagcggc acagattctg ctgcatttac acttcccggt
      121 gcgcaaccat acgaagtaag cttaaccgat ggagcggcag atcttgaaat atcattttcg
      181 ccgacaaaag taggcgcgca tacagcgaca ctaacattta gtacaatgtg cggtgaaaca
      241 acatatacca aaacagtttc attgtcagcc acaggtaaaa accattcaat aacaccactt
      301 atagcagata ctgctgatgt aactgaaggt gtagtaactc tatcgggtta tttgaagtat
      361 gcaggttgta aaactgatat tattgattat ggtttctatt actgcgaagg tgctgattgt
      421 acaccaagtt caagtagtga ttcaattcgc tcaagtgaac acactccaat tagctccgga
      481 agtagttggt ctgcacagcc ggaaggatta aaagataaaa catggtactc ttataagtct
      541 tatgcaaaaa gtactgctga tgcggatccg gtattatccg aagaaacagg tcacttctat
      601 gtaacttgcg attatccaat gggtgataca atttattata caattaatgc agcattaagc
      661 gaggatattc cttgcacgct tgagttttcg acatttgcag ctgctcttac gcatttgaaa
      721 tcaatgtcag aatatttagc ttcaagcagt ggtaatataa caaacaaatt acttaaacat
      781 atagtatttg aagtagaagc cggtgtatat ggcgaagcaa actcacaaat agatttgtct
      841 gacattaata aaattacaga caatggtcaa acaatagaat gcccgacgca tagtttaata
      901 gttcgtcgta aaaatgccac agatcgtcct acacttaatg gtttgaagtt gtatgagtct
      961 cgtcatataa caatagacgg attcaatata acacgtagta caacaacaag taatatgtca
     1021 ggagctacta tatcaattgg tagaaatagt gatagtaaca atgcagttcc gggtaatatg
     1081 aagtacgcca atgtaagtat tattaataac actattaccg caacaggttt ctgctgtgta
     1141 cacgtacagg gagctgatac tatttattac gaaaacaatg agttgtatgc tgatataacc
     1201 gcaacaggtg atcctgcaaa cgacagaaac tgggtgcttc aatgaagttt atgaattgca
     1261 agaatgtaca gattttgcgt aatagcttta gaggaactca tgcaacaaac ttgtttatac
     1321 aaggttcgcg taatttctta atcatgaata atgtattctg gaatgataat cagtatactg
     1381 aaaacgtagc atttgttcgt ccggtatatt ttggtggtga tggagaaaat aacgcacttc
     1441 aaaacattgg tatttattac aatacattct ttttgaaaga tgagggtggt agtacagatt
     1501 ataaagtaaa cttcatcgaa tttggtggag cggcacaaac gggcaatgct acttattatc
     1561 actcgaactt gatgggattc caatacaata actgttattc gtattcttta aaggtatcag
     1621 gtcgtgatac agacccattc ttgggtaaga atatggagac tgatatttgt ccgacattta
     1681 aggacaataa cttctggtct aaatacgatg tggctcaatc ggaacaagat acatcagcat
     1741 ttgcattcgg ttgtagtccc atctttatta atgttgaaaa tgctgtatgt tggactgcat
     1801 caaacgaccc ggcatcgctt gttataaaaa ctgatgcctt aaattttggt gtatctgtta
     1861 aaacggatat gtccggtatg aatattgcaa acacattgta caatgaccgt atatatccaa
     1921 gtaatttagc agatgcaatt cgtcctaaaa ctaattcatc tgaagaagaa ccgaatgagt
     1981 atggtatttt atcaaatgca cctactatta gaatgagtat gtcatcaatg gtagagacta
     2041 tatctcacga tgttgatatg acgtcttata ctttaactcc tagtagtaac gttaatttta
     2101 cattgtcagg tacaaatgca gacaaattct caataaataa atcgtctaca acgactgatg
     2161 ctacaggaac ttcaaaagat actgtaactg taacatttga tcgtcctgct accacaggta
     2221 catacaatgc atttttgaat atatctccat caacagtaag tgatgttgat actatagcaa
     2281 tacctcttat aggtatctat gctgatggcc ctgtttcggg aggttggaca ttaggtgcat
     2341 atcaacaaca cgaaggtaat gaggttgatg tgattgtttg gaatggcaca tcagattcgc
     2401 caaacgattg ggataatcgt ggtaactggg ttaagatgga tggaacgcga gttacttgtg
     2461 ttgataagtt gtcagaaaag ttgaaagtaa taatccctgc tcctgattca aaggtatatc
     2521 caatgcctgc aggcgataca attaagacat atcctaatat gccgacaaac tttaatgctg
     2581 aaaatcgttc agttaagcaa catgctgaac aagttgatgc aggtgcagga atcatagaaa
     2641 ccacaaaatt ctttgctgac agcatctatt tagagtacgg tgcttcgctt agaggtgttg
     2701 agaaattggt tcgcactgtc ggagaacaaa aagatacttt gtataatgtt gcaacaacga
     2761 actttactgc acaacgtagt gcatttaaag actctcgcga atggatactt gtaggaacgg
     2821 ttattaaacc atttacaggc aatggctatg aaacacgtaa tattaaatca ggtgatttct
     2881 atattgctaa tcaaacgcct cacgtatata tgcaacatat taaatgcact gacggcgtta
     2941 atgtaagttg gggtacacca ttctcaagtt tgcaagtaga agttcctaca acaacagcat
     3001 ttgcaataga gattcctgac caatacggac aatacaaatt gagttcatcg ctgttctata
     3061 aatattatta tcctgatgaa agcatgttga acgatggtac acaacctaaa tcattcaaat
     3121 ttgaaggacg ttttgctaat gatgcttcgt tgcctactta tagtattgta tcaggagtta
     3181 atttcttgaa taactcatat cctgctaatt tggatgcata tacactgcat acaagtgaat
     3241 catatccggg aacagtactt atgtatgatt atgaattgaa atcattccgt acacctcaaa
     3301 gtggtgaagt aattaagccg caaaacggat tcatatttaa tgctacatca acaacatcaa
     3361 tgacattagg tgccgatttg tttgtggatg gaaatacaag atacaaacgt gctttagacg
     3421 aaaaaccgaa tatctatatt agagcaataa atgcaggtag atcacttggt agtcagattg
     3481 ctcttgtata cgatgagttt aaggaacaga caatagatcc taatgttgat gctcgaaaac
     3541 catttaacaa ggaatcttct ttaagtcctg aattatatat aatggctcag gatgctgcat
     3601 ttgcttcatt gaatattaca aatatggaaa ctgtaattcc attaggttta cgtaatgcat
     3661 ctaagaagct tatggatgtt aagtttgtgt taagagcgca agaaggtttt ggatctgctg
     3721 ttttagttga ccgtcaggaa ggtaaggagt atgacttgct aacaggtgag gcttacacaa
     3781 ttacaaatat tgcagcagga acaatagaag gtcgtttctt cttgaactta agtggcattg
     3841 aagacccaga tcctattgat cctgagaatc cacctacgga tgttgaagat acagaacttg
     3901 taggagagtc aattagtatt tttgcacagg atggctcagt tgtaatttca tctaccgaag
     3961 gtgtagctct tcaaacggtt tatgtaagtg atatggcagg aaaaacagat acatacaaat
     4021 tatcgggaag ccattatcaa aaattggatt tacctgttgc gaaaggtgta tatctcattt
     4081 ctgtagtcgg agacacgatg acaaaagaac aaaaagtgat tattaagtga tattaaatct
     4141 atttggctat gtgaagaatc ttgcgtaatt ttgcgccgga ttctcacaat agctaataaa
     4201 tggagaatga taacgaatac aaattcagaa aatgaaaaaa catattaaat taataagcct
     4261 tttgttcttt ataataagca gtatgactgt tggggcggtt acactgccat caagttcata
     4321 tgtcccatcg ggagggaatg atggcggtta cgatccgata t
//
LOCUS       DAOPID010000123         4328 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_27547_length_4328_cov_3.501053, whole genome shotgun sequence.
ACCESSION   DAOPID010000123 DAOPID010000000
VERSION     DAOPID010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27547_length_4328_cov_3.501053"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="PK641_04895"
     CDS             complement(<1..692)
                     /locus_tag="PK641_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55719.1"
                     /translation="MMKKFNFILLIQVALFLSCSHTPERTAADLYQTAQQYFEQNNLN
                     AAKQVIDSIHTTFHNDVEVRKLARELMFKIQQKEEKRNVAYYDSMLLIKEPILAEYSK
                     QFILSKDSEYFDYKVYTHRSQTISYMPHTNILCEVKDNGELTLVSVFLGRKLDHTSFR
                     ITAKDIFAETFEVPLSSEYNYHFDDMGSRWEYVTFTPEYQNNVAGFIAQYADQALKVT
                     LKSQESSYNYFL"
     gene            772..2181
                     /locus_tag="PK641_04900"
     CDS             772..2181
                     /locus_tag="PK641_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPG55720.1"
                     /translation="MQAGWQNPIFDLISDCADAQQTECYVIGGYVRDWLLNRPSKDID
                     IVVVGSGIELAKALKAKLGRKASLSIFKNFGTAQIKYKDTEIEFVGARKESYRNDSRK
                     PVVENGTLEDDQKRRDFTINAMAICLNKSKYGELTDPFGGKQDLEYKILRTPLNPDVT
                     FSDDPLRMMRCIRFATQLKFSIEDGTYRALQTNKERISIISQERILDELNKIMMSEKP
                     SIGLDILYKCGLLEIIMPEISNLKGVEIISGKAHKDIFYHTLEVVDNVAKQSSDLWLR
                     WAALFHDVGKPATKRFDPKIGWMFHNHNYIGEKMIPHIFKRMKFPMNEKMRYVQKIVS
                     LHMRPIALVEEEITDSAVRRLLFEGGDDIEDLMTLCEADITSKNEAKVNKYMQNLQIV
                     RLKLKDIEQKDHVRNFQPPIDGAEIMKTFGLEPCGTVGKIKAIIKDAILDGKIPNEYE
                     PAHELMVKIAASMGLTPIK"
     gene            complement(2197..2871)
                     /locus_tag="PK641_04905"
     CDS             complement(2197..2871)
                     /locus_tag="PK641_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPG55721.1"
                     /translation="MAQLDRNIIKRLTQVDRQRWHFGFILGMNFADFVTQPSTSWKDE
                     DGNSWYSASAGLSPAFNVGMIIDLRCVEYLNIRFTPVLNLGERHLNYTCFNPAGERIG
                     DVKTTSVATTTIDIPFYIKYSAKRYGNVRPYIIAGGGPQFNLNLDPEVPILLNPFDVQ
                     IAFGIGLNIYTEYFKFCPEIKFAFGLLDQLNRNHPEMEGTDNILFTSSVDRLTSRMLI
                     ISFNFE"
     gene            complement(3001..>4328)
                     /gene="hisS"
                     /locus_tag="PK641_04910"
     CDS             complement(3001..>4328)
                     /gene="hisS"
                     /locus_tag="PK641_04910"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPG55722.1"
                     /translation="DFLPDEMAKRNYIFDTIKKVYALYGFKQIETPAMENLSTLMGKY
                     GEEGDKLLFKILNSGNFLDNVDEDDLKTKNSVKVISKISEKGLRYDLTVPFARFVVQY
                     RDQIKFPFRRFQIQPVWRADRPQKGRYREFYQCDADIVGSDSLLNELELLQIIDEVFT
                     QFGINVCIKLNNRKVLAGIAEVIGAPEKIIDITVAIDKLDKVGLDNVNAELAVKGLSA
                     DSVNKLQPILALKGDNASKVNDLRKILSTSEIGTKGVEELETIFNMLNESSVRCEIEL
                     DLTLARGLNYYTGTIIEVKSKDVQIGSITGGGRYDNLTGVFGMDGVSGVGISFGADRI
                     YDVLNQLDLYPKSETASTQVIFLNFGAKEAFYSLKCMKTLRTAGIACEIYPDSVKLKK
                     QMGYADECGIPYVAIIGETEMADNLISLKNMKSGEQEKLSIEALIAKLV"
BASE COUNT         1437 a          735 c          709 g         1447 t
ORIGIN      
        1 tctaaaaagt aattgtagct tgattcttga ctttttaaag tcactttcaa agcctgatca
       61 gcatattgtg caatgaatcc ggctacatta ttctggtatt caggtgtaaa tgttacatac
      121 tcccatctac tacccatatc atcaaaatga taattatatt cagaacttaa tggaacttcg
      181 aatgtctctg caaatatatc ttttgcagta atgcgaaatg aagtatgatc caatttgcgt
      241 cctaaaaaaa cacttactaa agtcagttcc ccattatctt taacttcaca taatatattt
      301 gtgtgaggca tataagaaat ggtttgacta cgatgtgtat atactttata atcaaaatat
      361 tctgaatctt tagacaaaat aaattgtttt gaatattctg ctaatatagg ctcttttatc
      421 aacaacatac tatcgtaata agcaacatta cgtttttctt ctttttgttg aattttaaac
      481 attaactcac gagccagctt acgtacttct acatcattgt gaaatgtggt gtgtatactg
      541 tcaataactt gtttggcagc attcaaattg ttttgttcaa aatattgctg agctgtttga
      601 tataaatcag cggcagttct ttcgggtgta tgagaacacg aaaggaacaa tgcaacttgt
      661 atcaataata taaaattaaa cttcttcatc atataaggtc acaaaattac tatttttttg
      721 tgagttcaag acaaacagcg tacctttgca gggttaataa attttaattc aatgcaagct
      781 ggctggcaaa atccaatatt tgacttgatt tctgattgtg cagatgcaca acaaacagaa
      841 tgttatgtta ttggaggata tgttcgtgat tggttactga atcgtccttc aaaagatatt
      901 gacattgttg tggttggtag cggaatagaa ttagccaagg ctttgaaggc aaaattaggc
      961 agaaaagctt cattaagtat ttttaaaaat ttcggaacgg ctcaaataaa atataaagat
     1021 accgaaatag aatttgttgg agctagaaaa gaatcttatc gtaatgattc gcgtaagccg
     1081 gtagttgaaa acggtacttt agaagacgat caaaaacgga gagattttac cattaatgca
     1141 atggcaatct gtctgaataa atctaaatat ggagaattaa cagacccttt tggaggtaaa
     1201 caggacttag aatataaaat tttacgaact cctttaaatc cggatgttac ttttagcgat
     1261 gacccattgc gaatgatgag atgcatacgc tttgcaactc aattaaagtt cagtatagaa
     1321 gacggtacat acagagctct tcaaactaat aaagaacgca taagtatcat atctcaagag
     1381 cgaattcttg atgaattgaa caaaattatg atgtctgaaa agccatctat aggattagat
     1441 attttgtata aatgcggcct actggaaatt atcatgcctg aaatctcaaa ccttaaaggt
     1501 gtcgaaatta tttccggcaa agctcataaa gatattttct atcacacact agaggtggtt
     1561 gataatgtgg ctaaacaaag ttctgatttg tggttaagat gggctgccct attccatgat
     1621 gttggtaaac ctgcaacaaa gcgatttgat cccaaaatag gttggatgtt tcataaccac
     1681 aactacattg gcgaaaagat gattccgcat atttttaaac gaatgaagtt tcctatgaac
     1741 gaaaagatgc gttatgtaca gaaaatagtt agtttacaca tgcgtcctat tgctctggta
     1801 gaagaagaaa ttactgattc tgccgttcgt cgtttattat ttgaaggagg tgatgatatt
     1861 gaagatttaa tgacactttg tgaagccgat ataacttcaa aaaatgaggc taaggtcaat
     1921 aaatacatgc agaatctgca aatagttaga ttaaagttga aggatataga acaaaaagat
     1981 catgttagaa atttccaacc gccaatagat ggagcagaaa ttatgaaaac atttggctta
     2041 gaaccttgtg gaactgttgg aaaaatcaaa gcaattataa aagacgctat tctggatggt
     2101 aaaattccta atgaatatga accggctcat gaattaatgg taaaaatagc agcttcaatg
     2161 ggattgactc ctataaaatg atctttttat ataatatcac tcaaagttga atgatattat
     2221 caacattctt gatgttaacc gatcaaccga gctcgtgaac agaatattat cagtaccttc
     2281 catttccggg tgattacgat ttaattgatc tagtaagcca aacgcaaatt ttatttcggg
     2341 acaaaatttg aaatattccg tataaatatt taaaccgatg ccaaatgcta tttgcacgtc
     2401 aaaaggatta agcaatatgg gcacttccgg atctaagttt aaattaaatt gtggacctcc
     2461 acctgcaatt atatacggtc gcacatttcc ataacgtttt gcactatatt ttatatagaa
     2521 aggaatatct atggttgttg ttgcaactga agtggtttta acgtctccta ttctttcgcc
     2581 tgcaggatta aaacaagtat agttaagatg gcgttctcct aagtttaaaa cgggagtaaa
     2641 tcgtatgttc aagtattcaa cacaacgtaa atctataatc ataccaacat taaatgcagg
     2701 tgataatcct gcagatgcag aataccaact gttaccatct tcgtctttcc agctagtgga
     2761 tggttgtgtt acaaaatcag cgaaattcat acccaaaata aatccaaaat gccatctttg
     2821 acggtcaact tgtgttaatc ttttgattat gtttcgatct aattgtgcca tcgacggaat
     2881 agcgactagc acaagtagca atatggttat gaatctgttt ttcaactata tatattttag
     2941 taacgaaata tgaactatgt aatattaact taacggaaat attatctgta tattttgttg
     3001 ttatactaat ttagcaatta aggcttcaat tgacaatttc tcttgttctc ccgatttcat
     3061 gttttttaga gaaattaaat tgtctgccat ttcagtttca ccaataatag ctacataagg
     3121 tattccgcat tcatctgcat agcccatttg tttttttaac ttaacagaat cgggatatat
     3181 ttcgcatgct ataccggctg ttctcaatgt tttcatacat ttcaaactat aaaaagcctc
     3241 ttttgctccg aaattaagga atataacttg tgttgatgca gtctctgatt ttgggtataa
     3301 atcaagttgg ttcaagacat cgtaaatacg atcagctcca aaagagatgc cgactcctga
     3361 tacaccatcc ataccaaata cacctgtaag attatcgtaa cgaccgccac ccgtaatact
     3421 acctatttga acatctttag atttaacttc tataatagtt cctgtatagt agtttaatcc
     3481 acgtgctaag gttaggtcta attctatttc gcatcgaaca gatgattcat tcaacatatt
     3541 aaatattgtc tctaattctt cgacaccttt agtacctatt tcagaagtac ttaatatttt
     3601 gcgtaaatca ttgactttag atgcattatc accttttaat gcaagtattg gttgaagttt
     3661 atttacagag tctgcggata aaccttttac tgctaattct gcattaacat tgtctaagcc
     3721 taccttgtct aatttgtcaa ttgcaacagt aatgtcaata attttttcag gagcccctat
     3781 cacttctgct attcctgcca atacctttcg attatttaat ttgatgcaaa cattaatacc
     3841 gaattgtgta aatacctcgt ctattatttg caataattcc aattcgttaa gcagagaatc
     3901 acttccaaca atatcagcat cacattgata gaactcacga taacgacctt tttgtggacg
     3961 atcagctctc caaacaggtt gtatttggaa tcttcgaaaa ggaaatttta tttgatcgcg
     4021 atattgtacc acaaatcttg caaacggaac tgttaggtca tatctcaatc ctttttcaga
     4081 tatttttgat attactttaa cagaattctt tgtttttaaa tcatcttcat caacattatc
     4141 taaaaaattg cctgaattta gaattttaaa aagcaactta tcaccttctt ctccatattt
     4201 tcccatcaaa gttgatagat tttccattgc aggagtctct atttgtttga aaccatataa
     4261 agcatatact tttttaatag tatcaaaaat gtagttgcgt tttgccattt catcaggcaa
     4321 aaagtcgc
//
LOCUS       DAOPID010000124         4316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_27660_length_4316_cov_3.829617, whole genome shotgun sequence.
ACCESSION   DAOPID010000124 DAOPID010000000
VERSION     DAOPID010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27660_length_4316_cov_3.829617"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PK641_04915"
     CDS             <1..309
                     /locus_tag="PK641_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HPG55723.1"
                     /translation="SLKTALTAQKYNDAIKEMLEIVGLDRMVTILNPKTRTEEKYKFY
                     EIVSSHMARRSFIGNLYKQVQDPNLIGSMSGHVEGSRAFARYRQIDDDIKKNIISLLE
                     "
     gene            351..569
                     /locus_tag="PK641_04920"
     CDS             351..569
                     /locus_tag="PK641_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPG55724.1"
                     /translation="MATTVLNETQLQLVKMFSFAKTKTATDKLKKVLSSYYAKEIEKQ
                     MDALWKSGKMTEEKNNKIAKTHLRTAYK"
     gene            complement(916..987)
                     /locus_tag="PK641_04925"
     tRNA            complement(916..987)
                     /locus_tag="PK641_04925"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(952..954),aa:Arg,seq:ccg)
     gene            1165..2202
                     /gene="asnA"
                     /locus_tag="PK641_04930"
     CDS             1165..2202
                     /gene="asnA"
                     /locus_tag="PK641_04930"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HPG55725.1"
                     /translation="MSQLVKPQNYHSYLDLRQTELAIKNLKDFFQSSLSAELRLRRVT
                     APLFVLKGMGINDDLNGVERPVTFCVKDLNEAQAEVVHSLAKWKRLTLADYKIERGYG
                     IYTDMNAIRADEELDNTHSLYVDQWDWEMVIEDQDRNLAFLKDIVRRIYATIVRCEYA
                     VYEHFSEIKPILPSKIHFIQAEELLSLYPDKTPKERENLITKKYKAVFIIGIGSNLSN
                     GEKHDGRAPDYDDWTTLNEDGYKGLNGDILVWDNVLGQAMELSSMGIRVDKKALLHQL
                     DITGQQDRMKYYYHKCLVEDKLPLSLGGGIGQSRLCMFLLRKAHIGEIQSSIWPESMR
                     KECDESGICLL"
     gene            2207..2887
                     /gene="mtgA"
                     /locus_tag="PK641_04935"
     CDS             2207..2887
                     /gene="mtgA"
                     /locus_tag="PK641_04935"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HPG55726.1"
                     /translation="MKQFFRIILFAICVFVIESILSVAAYRFVPVVYTPLMFIRVAQQ
                     AIDGKPLALHREWTSLDNISHNMVQAVVASEDNLFTEHYGFDFKGIEKAFKNNQRGKR
                     LKGGSTISQQTAKNVFLTPSRSYVRKAFEAYYTILIELIWSKSRIMEVYLNVIETGNG
                     MYGVTVASEEYFHKKPAKLTKYQAASIAASLPNPRKYKVVNQGPYMQRRTSHIQSLMN
                     KIERVSFK"
     gene            complement(2944..>4316)
                     /gene="glmM"
                     /locus_tag="PK641_04940"
     CDS             complement(2944..>4316)
                     /gene="glmM"
                     /locus_tag="PK641_04940"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HPG55727.1"
                     /translation="SISGIRGTIGGQVGSGLSPVDVVKFTAAYSTLIRKTTKNKSNKI
                     VVGRDARLSGNMVDKLVVGTLMGMGFDVCEIGLASTPTTELAVTMENAAGGIIITASH
                     NPKQWNALKLLNDKGEFLNKEEGEEVLRIAESDEFSFSEVDDLGLVTECDYTQKHIDK
                     VLSLKLVDVDAIKKAKFKVAIDCVNSVGGIILPRLFDALGVETLTQLYCEPTGNFCHN
                     PEPLEKNLGDIMSLMKKGGNDVAFVVDPDVDRLAMICENGEMYGEEYTLVTVADYVLT
                     HTPGNTVSNLSSTRALRDVTRSHGGSYQAAAVGEVNVVTKMKESNAVIGGEGNGGVIY
                     PECHYGRDALVGIALFLSHLAHKKMKVSELRASYPSYFISKQRVDLTPSIDVDAILNK
                     VKEKFHNEEINDIDGVKIDFADKWVHLRKSNTEPIIRIYSEASTQEKADELGAEIIAI
                     IRGFIS"
BASE COUNT         1467 a          708 c          756 g         1385 t
ORIGIN      
        1 tcactaaaaa cagcattaac agcgcaaaag tacaatgatg ctatcaagga aatgttagaa
       61 attgttggat tggatcgtat ggttacaata ctaaacccaa agacacgtac cgaagaaaaa
      121 tataagtttt atgaaattgt aagtagccac atggcgcgta gatcttttat aggcaacctg
      181 tataaacaag ttcaagatcc taatctaatt ggatctatgt ccggacacgt agagggttcg
      241 cgtgcttttg cgcgttaccg gcaaattgat gacgatataa agaagaacat tatcagcttg
      301 ttagaataat tgttttttta tatctttgca aaaaatagga gggctaaatt atggcaacga
      361 cagtattaaa cgaaacacaa ttacaacttg taaaaatgtt ttcttttgct aaaacgaaaa
      421 cagcaacaga caaacttaaa aaagtgcttt cttcttatta tgctaaggaa atagaaaagc
      481 aaatggatgc actgtggaaa tcgggaaaaa tgacagaaga aaaaaataat aaaatagcga
      541 aaacccattt gcgcactgca tacaaataat gaagaatata gtattagata ccaattgttt
      601 tataaataac ctcaccccac ccaattaagc ctttatccaa aggctttttt tattctaacc
      661 ttaccacacc tataacgata gacaatgagc ggatttcaga aataggttaa taggaattgt
      721 tttgttcact atcggcaaaa ttgcttttaa aatttacaaa acctttgttt tgcatatata
      781 aaagcatata tttgcaagac aatatttgcc gtataaaatt agactgtaaa atataaaagc
      841 attgcaaata tcgtgcaaaa cttaaaataa atcatcttaa tttattaata atcagcatat
      901 tactttctaa tattgtgacc ttgtggggag tcgaacccca atcgaaagaa ccggaatctt
      961 ttattctatc cattgaacta caaggcctat tgagcgcaaa ggtaattaaa atatcgcttg
     1021 tatattacaa taaacattat tttgtctttt tgttatttta aatcgatatt ttgtcacttt
     1081 tgttgcgtta atttttaaat aatgctatct ttttatcaat aaatatatta attttgcgtc
     1141 aaatataaat caaaatataa ttgtatgtca caactagtaa aaccgcaaaa ctatcattca
     1201 tatctggatt tacgacagac tgagctggca atcaagaact taaaagattt ctttcagtca
     1261 agtttatcag ccgaattgcg tttgagacgt gtaactgctc ctttgtttgt tctcaagggt
     1321 atgggtataa atgatgattt gaatggggta gagagaccgg ttacattttg tgttaaggat
     1381 ttaaatgaag cacaagcaga agttgtacat tcgttagcaa aatggaaacg acttacattg
     1441 gcagattata aaattgaaag aggatatggt atatatacag atatgaatgc aatacgtgct
     1501 gatgaggaat tggataatac gcattcacta tatgtagacc aatgggattg ggaaatggtt
     1561 atcgaagatc aagacagaaa cttggcattt ttgaaagata tagttcgtag aatttatgca
     1621 acaatagtaa gatgtgaata tgcagtatat gaacatttct ctgagataaa accgatttta
     1681 cctagtaaga ttcattttat tcaggcagaa gaattgttat cattatatcc tgataaaact
     1741 cctaaagaac gtgagaattt aattactaaa aagtacaaag ctgtatttat aattggtatt
     1801 ggttcgaatt tgtctaatgg agaaaaacat gatggtagag ctcctgacta cgatgattgg
     1861 acaactttaa atgaagatgg gtacaaaggt ttaaatggag atatattagt atgggataat
     1921 gtattaggac aagctatgga attgtcgtca atgggtatta gagttgacaa aaaggctttg
     1981 ttgcatcaat tggatattac aggtcagcaa gaccgtatga aatattatta tcataaatgt
     2041 ttagttgaag ataaattacc attatcacta ggcggaggta ttggacaatc tcgcttatgt
     2101 atgttcttac tacgcaaagc ccatataggt gaaattcaat cgagtatttg gcctgaatct
     2161 atgagaaaag aatgtgatga atccggtatc tgtttgttgt aataatatga aacaattttt
     2221 ccgcattata ttgtttgcaa tatgtgtgtt tgttatagaa tctatattgt cagttgccgc
     2281 gtatagattt gttcctgttg tatatactcc actgatgttt atcagagtgg ctcaacaagc
     2341 aatagatgga aaaccgcttg ctttacacag agaatggact tcattggata atatttcgca
     2401 taacatggta caagcagttg tagcatcaga agataactta tttactgaac actatggatt
     2461 tgattttaaa ggcatagaaa aagcctttaa gaataatcaa cgtggaaaac gtttgaaagg
     2521 tggaagtaca ataagtcagc aaactgcaaa gaatgtattt ttaacaccat ctaggagtta
     2581 tgtgcgaaag gcattcgaag cctattatac aattttaatt gagcttattt ggtctaaaag
     2641 tcgtataatg gaggtttatt taaatgtaat tgaaacagga aatggtatgt atggtgtaac
     2701 agtagcttcg gaggaatatt ttcataaaaa accggctaaa cttaccaagt atcaagctgc
     2761 ttctattgca gcgtcattac ccaatccaag aaaatataag gttgtgaatc aaggtccata
     2821 tatgcaaagg cgcactagtc atattcaaag tttaatgaat aagattgaac gtgtaagttt
     2881 taagtgatga ctttgaaaat aaagtaatag taaaggcaaa cttcaatgaa gtttgccttt
     2941 gtgttatgag atgaatccgc gtataatagc aataatctct gcacctaact cgtcagcttt
     3001 ttcctgagtc gatgcttcag aatagatacg aataataggt tctgtatttg acttacgtag
     3061 atgaacccat ttatctgcga aatcaatctt aactccatca atgtcgttta tttcctcatt
     3121 gtggaatttt tcttttactt tatttagaat cgcatcaaca tcaatagatg gagttaaatc
     3181 aactctttgt tttgagataa aatatgaagg atatgaagca cgtaattctg ataccttcat
     3241 ctttttgtgt gccaaatggc ttaaaaacaa agcaatacca accaatgcat ctctaccata
     3301 atggcattca ggataaataa cacctccgtt accttcgcct ccaataacgg cattactttc
     3361 tttcattttt gttacgacgt taacttcacc aacagcggca gcttgatatg aacctccatg
     3421 tgagcgtgtt acatcgcgta aagctctggt agaacttaaa ttagaaacag tattaccggg
     3481 tgtatgagtt aatacataat cagcaactgt aaccaatgtg tattcttcgc catacatttc
     3541 tccattttcg catatcattg ctaatctatc aacatcagga tcgactacaa atgcgacatc
     3601 gttaccacct tttttcatta atgacattat gtcgccaaga ttcttttcta gaggttcagg
     3661 attgtggcag aagttacccg taggttcaca atataattga gtcagagttt cgacgcctaa
     3721 tgcatcaaat aaacggggaa gaattattcc tccaacagaa ttgacacaat caatagctac
     3781 tttaaactta gcttttttaa tcgcatcgac atctactaac ttcaatgaca gtactttgtc
     3841 aatatgtttt tgtgtgtaat cacattcagt aacaagaccc aagtcgtcaa cttctgaaaa
     3901 tgaaaattca tcgctttctg caatgcgaag aacttcttct ccttcttctt tgtttaagaa
     3961 ttcgccctta tcattaagta gttttaaagc attccactgt ttaggattgt gacttgctgt
     4021 gatgataatt ccacctgcag cattttccat tgttacagct aattctgtag taggagtgga
     4081 tgctaaacct atttcacaaa cgtcgaatcc catccccata agagtgccaa ctactaattt
     4141 atctaccatg ttgcccgaca aacgagcatc acgtccaaca actattttat tacttttgtt
     4201 tttagtcgtt ttacgtataa gagttgaata tgccgctgta aattttacta catctacagg
     4261 acttagacca cttcctactt gtccacctat ggtccctcta attccggaaa tcgatt
//
LOCUS       DAOPID010000125         4300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_27789_length_4300_cov_4.769376, whole genome shotgun sequence.
ACCESSION   DAOPID010000125 DAOPID010000000
VERSION     DAOPID010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27789_length_4300_cov_4.769376"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1298
                     /locus_tag="PK641_04945"
     CDS             <1..1298
                     /locus_tag="PK641_04945"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPG55728.1"
                     /translation="LGYVTGMMLVYPFEKAAYDAKIGKVVGPIRTRFGYHLILVTNRR
                     PSKGEIKVAHIFKRKPEKADSASIDSIRTFVNSIYKLASAGSDFAELAKKYSEDEANA
                     SQGGVLPWLSIGRTNETFETAAFALKDSGAISKPVEASYGWHIIRLLEKRPIQSLDDS
                     RQQIESRINGDERANIINTSFINKIKKTYNYKAGKGDTIATFANVVLTKDNLNKFSAA
                     RPRNSDSLTQFINTSLIDYENSNLEQRYPEFGMLMKEYYDGILLYYISKEKVWDKASK
                     DTAGLRMQFEQNKEKYRWKKPHYKGIIVYCASKEIKDKAADIIKTVPKDSVSQYLKRQ
                     LNVNKTINVKIESGIYEQGRNQVIDALVFNEGSLPINEKFPEVLVDGYIQKEYPETYK
                     DIKGPVSNDYQEILEKSWIDSLRKTYPVIIYDNILKTVK"
     gene            1295..2158
                     /locus_tag="PK641_04950"
     CDS             1295..2158
                     /locus_tag="PK641_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55729.1"
                     /translation="MRRHKIIVLTTIVVIAFCSCNKVVQLDPTKNHIMEVNGNVLSYD
                     DIVKVMPEDLSGQDSAIFVENYKKRWATNILMYDKALRNVGSTDEINELAEIYRRELI
                     INEYQQQLIDQEMPELNEDSIMAFYEKQKEHFPLEEAIVKGIFIKILTSTPNQSKLSR
                     WLSNINDENLDNIMRFCTKTAIYYDFFNDNWVYYSKISSLLPEKMDANDPALSRGTVV
                     LQSDEYSYYLKIIGICQAGEPKPYEFIRPELMNIMANKQKIDFIHNFQEDLYQKALKN
                     NTIITIEDKNK"
     gene            2161..3513
                     /locus_tag="PK641_04955"
     CDS             2161..3513
                     /locus_tag="PK641_04955"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPG55730.1"
                     /translation="MKKTTYITFLLICITSLMPILSSNQIIDQVVWVIGDEPILKSDI
                     ETEILRMRYEKQSIDGDPYCVIPEQIAIQKLFIAQAKLDSITVSEASVEQQVEGRIKY
                     FIQRIGSKEKVEEYFNKPIAKIKEEMTRTVEEQLLVQEMQQKITSSNKITPADVKRFY
                     NELPKDSIPTIPEQVEVQIISIAPQTTPIEEERIKSELRDFREKIESGEYQFSTLAIL
                     YSEDRASAMQGGELGFMSKGKLVPEFANVAFSLYDPKKVSRIVETEFGYHIIQLIERK
                     NDLVNCRHILLIPKVDNKQKDAAKLKLDSIAEDIRTGKFSFEEAALYSSEDKDTQQNE
                     GLMINPTNGSTKFQLQDLPADIAKTVYNLQIGEISRPFVYINEKGKELVSIIRLKSKI
                     KSHVANPQDDFQELKNIVTVKKNQELIEDWIKDKQKDTFIHINEEYKDCKFRYPGWIQ
                     "
     gene            3594..>4300
                     /locus_tag="PK641_04960"
     CDS             3594..>4300
                     /locus_tag="PK641_04960"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HPG55731.1"
                     /translation="MEHSETLSFDQERMPDCQILGGNVQFRHDNALMFCDSAFFHDKQ
                     NTIDAFGNIRIEQGDSIFVYGDKLHYEGNIKLAKLRHHVRMIKDGMTLYTDSLNYDRE
                     LNVGYYFNKGKIVDSTNVLTSINGHYYPNTNTAVFQKDVVLTNPDFILYSDTLKYNTA
                     TDIAYILGPSTILYDSTTIYSEYGWYDTKQEHSKLIDNSIIKDKEGRTLTADTIYYNR
                     KNNIAKGYINVQLRDSV"
BASE COUNT         1691 a          672 c          755 g         1182 t
ORIGIN      
        1 gtttaggata tgttaccgga atgatgctgg tttatccatt tgaaaaggcc gcatacgatg
       61 caaaaattgg gaaagtagta ggacctataa gaactaggtt cggatatcat ctgattttag
      121 ttacaaatcg cagaccaagt aaaggcgaaa ttaaggttgc acatatattt aaacgtaaac
      181 ctgaaaaagc agactcggca agcattgaca gcataagaac atttgtaaat tctatttaca
      241 aattggctag tgccggaagt gattttgcag aacttgcaaa aaaatattcg gaagatgaag
      301 ctaatgcgtc acaaggtgga gtattgccat ggttaagtat aggacgcaca aatgaaacct
      361 tcgaaactgc tgcatttgca ttaaaagaca gcggagcaat ttctaaaccc gtagaagcat
      421 catacggatg gcacataatt agacttttag aaaaacgccc tatacaatct ttagatgatt
      481 ctcgtcagca aattgaatca cgtataaacg gagacgaacg cgcgaatatc attaatacca
      541 gttttatcaa taaaattaaa aaaacataca attataaagc cggtaaaggt gatactatcg
      601 ccacattcgc gaatgttgta cttacaaaag acaatttgaa taaattttct gctgcaagac
      661 cacgtaattc tgatagttta acacaattca taaatacttc tctgatagat tatgaaaata
      721 gtaatttgga acaacgttac cctgaattcg gtatgttgat gaaagagtat tacgacggca
      781 tattactata ttacataagt aaagaaaaag tatgggataa ggcatcaaaa gacactgcag
      841 gacttagaat gcaatttgag caaaacaaag aaaaatatcg ttggaaaaag cctcactaca
      901 aaggtataat tgtatattgc gcatctaaag aaataaaaga caaagcagct gatatcataa
      961 aaacagttcc taaggattct gtatcgcaat atctaaagag gcaattaaac gtaaataaga
     1021 caataaacgt aaaaatagaa agtggcatat atgaacaagg cagaaaccaa gtaattgatg
     1081 ctttggtatt taacgaaggt tcacttccta ttaacgaaaa atttcctgaa gttcttgtag
     1141 atggatatat tcaaaaggaa tatcccgaaa catacaaaga tatcaaggga cctgtatcta
     1201 atgattatca agaaatttta gaaaaatcat ggatagatag tttacgtaaa acatatcctg
     1261 tgattatata cgacaacatt cttaaaaccg ttaaatgaga cggcataaaa taatcgtttt
     1321 aaccacaatt gtggtaatag ccttttgttc ttgcaataag gttgtgcaat tggatcctac
     1381 taagaatcac attatggagg tcaatggcaa tgtattgagc tacgatgaca ttgtaaaagt
     1441 gatgcctgag gatcttagtg ggcaagatag tgcaattttt gtagagaact acaaaaaaag
     1501 atgggcaaca aatattttaa tgtatgacaa agcactaaga aatgtcggaa gcactgatga
     1561 aattaatgaa ctagctgaaa tatatagaag agagcttatt attaatgagt atcagcaaca
     1621 attaattgat caagagatgc cggagttaaa cgaagatagc ataatggcat tttatgaaaa
     1681 acaaaaagag cacttcccct tggaagaagc cattgtaaaa ggaatcttca ttaagatact
     1741 tacatcaact ccgaatcaat caaaactttc tcgctggtta tctaatataa atgatgaaaa
     1801 cttagataac attatgcgct tctgtacaaa gacagcaata tactacgatt tttttaatga
     1861 taattgggtg tattactcga aaatttcatc cctacttcct gaaaagatgg atgcaaatga
     1921 tcctgcatta tcaagaggaa ctgtagtttt gcaatctgat gagtatagtt actatttaaa
     1981 aataataggt atatgccaag ccggagagcc aaaaccatac gaatttatta ggccggaatt
     2041 aatgaatata atggctaaca aacagaaaat agattttatt cataattttc aagaagatct
     2101 ataccaaaag gcactcaaaa acaatacaat tattacaatt gaagacaaaa ataaatagac
     2161 atgaaaaaaa caacatatat aacatttcta ctaatatgca taactagttt gatgcctatt
     2221 ttgtcaagca accaaattat tgaccaagtt gtatgggtaa tcggtgatga gcctatattg
     2281 aaatcggata ttgagacaga aatattgcgt atgagatatg aaaaacaatc aatagatggt
     2341 gatccatatt gcgtaatacc tgaacaaata gcaattcaga aattatttat tgcacaagct
     2401 aaactagata gtataactgt atccgaagct tctgttgaac agcaagtaga aggtcgtata
     2461 aaatatttta ttcaacgtat tggttcaaag gaaaaggttg aagaatattt caataaaccg
     2521 attgcgaaaa ttaaagaaga aatgacacgc actgttgaag aacaactctt ggtacaagaa
     2581 atgcaacaaa agattaccag tagcaacaaa ataacgccgg ctgacgtaaa aaggttttac
     2641 aatgaacttc caaaagatag tatccccaca atacctgaac aagttgaggt tcaaataatt
     2701 tcaattgctc ctcaaactac tccaatagag gaagaacgta ttaaaagcga attaagagat
     2761 ttccgtgaaa aaatagaatc aggtgaatat caattctcaa ctttggctat tctttattcg
     2821 gaagaccgtg catctgcaat gcaaggcggt gagttaggat ttatgagtaa aggaaaatta
     2881 gtgcccgaat ttgcaaatgt tgcattctct ttgtacgatc caaagaaagt ttcacgcatt
     2941 gtagaaactg aatttggata tcacatcatt caactaattg aacgtaaaaa cgacctagtt
     3001 aattgtagac atatattact aattcctaag gttgataaca agcaaaagga tgctgccaaa
     3061 ttaaaattag atagcattgc cgaagatata agaacaggta aattcagctt cgaggaagct
     3121 gcattatact catctgaaga caaagataca cagcaaaatg aaggattaat gattaatcct
     3181 acaaatggtt ctacgaaatt tcaactacaa gacttacctg cggatattgc aaaaactgtg
     3241 tacaacttac aaattggcga aatttcaaga ccgtttgttt acatcaatga aaaaggcaaa
     3301 gaactggttt caataatccg actaaaatca aaaatcaaaa gccatgttgc caatccacag
     3361 gatgattttc aggaattaaa aaacattgta actgtaaaga aaaaccaaga acttattgaa
     3421 gattggatta aagacaaaca gaaagataca tttatacata taaacgaaga gtataaagat
     3481 tgtaaattta ggtatcccgg gtggattcaa tagaaatgac aaaaaaaaca ctcatttacg
     3541 ttatatgttc cgttttttta attgaggttt ttgccgaaaa aaccactatc tatttggaac
     3601 attccgaaac tttaagcttt gatcaagaac gtatgcccga ttgccagatt cttggtggta
     3661 acgtgcaatt ccgacatgat aatgcactaa tgttctgcga tagcgcattt ttccatgata
     3721 aacaaaatac aatagatgcg tttggcaata ttcgcattga acaaggagat agtatatttg
     3781 tatatggaga caaactgcat tacgagggaa atatcaaatt ggcaaaattg cgtcatcatg
     3841 tacgaatgat aaaagatggc atgacacttt atacagattc cctaaattac gatagagaac
     3901 taaatgttgg atactatttt aataaaggaa aaattgttga tagcacaaat gtattgacat
     3961 caattaatgg tcactactac cctaacacaa atacagcagt atttcaaaaa gatgtagtgt
     4021 taacaaaccc ggattttatt ctgtactccg acaccttaaa atataatacc gcaacagata
     4081 ttgcttatat acttggtcca tcaacaattt tatatgacag taccacaata tactcagaat
     4141 atggttggta tgacacaaag caagagcact caaaacttat agacaattca attattaaag
     4201 ataaggaagg aagaacactt acagccgata caatctacta taatagaaaa aataacatag
     4261 caaaaggtta tataaatgtt caattgcgag actctgtgca
//
LOCUS       DAOPID010000126         4273 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_28006_length_4273_cov_4.453532, whole genome shotgun sequence.
ACCESSION   DAOPID010000126 DAOPID010000000
VERSION     DAOPID010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28006_length_4273_cov_4.453532"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(229..1569)
                     /locus_tag="PK641_04965"
     CDS             complement(229..1569)
                     /locus_tag="PK641_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HPG55732.1"
                     /translation="MEKRGGFATKIGILMATVGSAVGLGNIWRFPYETGRNGGAAFLL
                     VYLLSIFIIGFPVVMAEFVIGRKAKANPVGAFKKLAPNAKWHWVGYMGVLAGLLILGY
                     YSVVAGWTMEYVFKALRFELENKSAADYKSMFETFTSNPILPIIWTVLVMIISHLIVK
                     KGIKGGIEKASKIMMPILFLLMLILAVHSILLPGSMKGIEFFFNPDFSKLTAESVLTA
                     LGHAFFSLSLGMGCLITYASYFKSDIDLPKTAAQVTIIDTLVSVTAGVIIFPAVFAFN
                     MQPEAGPGLVYMVLPNVFQQMSGSFIWSTMFYILLFLAALTSLISLHEVVTAYIHERY
                     SISRSNSSRIVTALVLFLGVFCSLSFGLMKNATLFSMNFFEWLEYLTASVLLPLGGML
                     ISIFVAWYLDKKIVSDELSNDGKVKVKGFKIYIFILRYVAPLCIFAVFVTSIIK"
     gene            1727..2077
                     /locus_tag="PK641_04970"
     CDS             1727..2077
                     /locus_tag="PK641_04970"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPG55733.1"
                     /translation="MKDPYLLSDMELARQIGMRIRECRLDQNISQQKLAKAAEVSLSS
                     VIRIEDGQIKSFDTFIRILRTLGLLEIVSTLLNRDPISPSEYYKLVKSAKQTKRKRAA
                     KKIITENNKKESEW"
     gene            2071..3351
                     /locus_tag="PK641_04975"
     CDS             2071..3351
                     /locus_tag="PK641_04975"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HPG55734.1"
                     /translation="MVEVAEVKMYSRVVGYVKWDNQYDLAAFEYDKEFLNLGIEPAPI
                     MMPVKEGRIYRFGDLGKDTFKGLPGMLADSLPDTYGRALFDKWLTLTGNVSSNSIESL
                     CFMGKRCMGALEFEPAITKQSEGNSQIEIQSLINVAKEALNEKSNFKTNLSDDQKTAI
                     AEILRLGTSAGGQRAKAIIAYNKATGEIRSGQVDAPAGFDHYIIKLDGVSSKLELKET
                     GNYGRLEYSFSELAKLCNIQMTECSLIEENGRAHFMTKRFDRNGGKKIHLQTLCGIAH
                     YDYRLFHAYSYEQAFSVMRTLRLSYEEAQEMFKRMVFNIVIRNNDDHTKNISFLMDED
                     GKWRLSPAYDIGYAYNPNGKWTSHHQMSINGKFDKITKTDLLECAYKNNIKNAQQIIG
                     EVCDAASKWNKIAQECDIPKTIINNIIPNLQLNL"
     gene            3556..>4273
                     /locus_tag="PK641_04980"
     CDS             3556..>4273
                     /locus_tag="PK641_04980"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HPG55735.1"
                     /translation="MTMKAKPFIKWVGGKTQLIESIEKALPENFAEIKNVTYIEPFVG
                     GGAILFWILQQYPNITKAVINDINPDLTTAYKTIKETPTELVKYLQIIQDEYLPLDEE
                     KRKEYYLNKRERFNTKSLESIENTALFIFLNRTCFNGLYRVNSKGLFNVPFGRYANPK
                     ICDKQTIFADSELLQKVEILTGDFENTLKYATNNSFFYFDPPYKPLSDTSSFNSYAKE
                     DFNDLEQIRLGNFCKKIDLLG"
BASE COUNT         1589 a          734 c          705 g         1245 t
ORIGIN      
        1 accgaatcaa actgctgaat agcatttagt tcgggttcaa tgttgtcatt ttgatactgc
       61 gctaacacat aagaactgca tagcttgata caaactaaaa ttaagataat tgatagcagt
      121 gcaagcactc cgcgcctttc ctgcttagta aagaagaaat aatctttcca ctccataaga
      181 cataaaaatt aagatgttga tattatattt acggctgcaa atataaggct attttataat
      241 actggtcaca aatactgcaa aaatacacaa aggtgccaca tatctaagta taaatatata
      301 gattttaaaa ccttttactt tgacttttcc atcattactc aactcatcag atactatttt
      361 cttatctaag taccaagcta caaaaataga tatcagcatt cctcccaatg gtaataatac
      421 tgaggctgta aggtattcga gccactcaaa aaagttcatt gaaaacaaag ttgcattttt
      481 catcagacca aacgacaacg aacaaaaaac acctaagaat aaaaccaaag cagttactat
      541 gcgtgatgaa tttgatctac ttattgaata tcgttcatga atatatgcgg taacaacttc
      601 atgaagtgat atcagtgaag ttaatgccgc caaaaacaat aatatataaa acattgtaga
      661 ccagataaaa gaaccggaca tctgttgaaa aacgtttggc aaaaccatgt aaactaaacc
      721 cggacctgct tcgggttgca tgttgaaagc aaaaacggcc gggaatatta ttacacctgc
      781 agttacagaa accaatgtat caataattgt tacttgagcg gccgttttgg gcaaatcaat
      841 atcactttta aaataagaag catatgtaat taagcatccc attcctaaac ttagcgagaa
      901 aaatgcatga cctaaggccg taagaactga ttcggctgta agtttagaaa aatcagggtt
      961 aaagaaaaat tctattcctt tcatactgcc tggaagtaat atggaatgaa ctgctaaaat
     1021 caacatcaat aaaaacaaaa tcggcatcat aatttttgag gctttctcta ttcctccttt
     1081 aatgcctttt ttaacgatta aatgggatat aatcatcacc aatacagtcc atattatagg
     1141 aagaatggga ttagacgtaa atgtctcgaa catagattta taatctgctg ccgatttatt
     1201 ttcgagttca aagcgtaaag ccttaaatac atattccatt gtccatcctg caacaaccga
     1261 ataatatcct aaaatcaaca aacctgctaa aactcccatg taacctaccc aatgccattt
     1321 tgcgtttggt gctagttttt taaatgctcc taccggatta gctttggcct tacgccctat
     1381 cacaaactca gccattacta caggaaaacc tatgataaat atactcaaaa gatataccaa
     1441 caaaaaggca gcaccaccat ttctacccgt ttcgtatgga aatcgccata tattgcccaa
     1501 accgacagca gagcctactg ttgccattaa aatacctatt tttgttgcaa aacccccgcg
     1561 tttttccatt tttgaatttt aatagggcaa aactactaaa aatcctggaa atgacaaatg
     1621 tatgataata cacggcttaa cgcacttatg caaaacattt tatataatat gtcgtttttg
     1681 aaatattata tatatatttg catcataata tatcaaaata gacaatatga aagatccgta
     1741 tctattatcc gacatggagc ttgcacgtca aataggaatg agaatacgag aatgcaggct
     1801 cgatcaaaac atatcccaac agaaacttgc aaaagcagca gaagtatcct tatcatccgt
     1861 tattagaatt gaagacggtc aaataaaatc gtttgatact tttattcgca tattgcgtac
     1921 attaggttta ctcgaaatag taagtacact gcttaacaga gatcctataa gcccgagcga
     1981 atattacaaa ttagtaaaat cggcaaaaca aacaaagcgt aaacgcgccg caaaaaaaat
     2041 catcacagaa aacaataaaa aagaatcgga atggtagaag ttgcagaagt aaaaatgtat
     2101 agtagggttg ttggttatgt aaaatgggat aaccagtatg acttggctgc atttgagtat
     2161 gataaagagt ttctaaattt aggaattgag ccggctccta ttatgatgcc tgtaaaagaa
     2221 ggccgcatat atcgttttgg agatttaggg aaagacacat ttaaaggttt acccggaatg
     2281 ttagccgact cattgcctga tacttatggc agagccttat ttgataaatg gcttacttta
     2341 acaggtaacg taagcagtaa ttcaatagaa tcactttgtt ttatgggaaa gcgctgcatg
     2401 ggtgcacttg aatttgagcc ggctattact aagcaatcag aaggaaactc gcaaatagaa
     2461 atacaatctt tgataaatgt tgctaaagaa gctctaaatg agaaatcgaa ttttaaaact
     2521 aatttatctg atgatcaaaa aacagccatc gctgaaatac taagattggg aacatctgcc
     2581 ggaggacaac gcgcaaaagc aattattgcc tacaataaag caaccggcga aattcgatcg
     2641 ggtcaagttg atgctcctgc cggtttcgat cattacatta ttaagctcga cggcgtttct
     2701 tctaagcttg aattaaaaga aaccggaaac tatggtcgtc tggaatattc attctcagaa
     2761 ttagcaaagt tatgcaatat acaaatgacc gaatgttctt taattgaaga aaacggcaga
     2821 gcacatttta tgactaaacg ctttgatcgc aatggcggaa aaaagattca tttacaaaca
     2881 ttgtgtggca tagcacatta tgattatcgc ttatttcatg cctactctta cgagcaagct
     2941 tttagcgtta tgcggacttt gcgtttatca tacgaagaag ctcaagaaat gtttaagcgt
     3001 atggtattta atattgtaat aagaaataat gacgaccaca caaagaatat atctttttta
     3061 atggacgaag atggtaaatg gcgattatct ccagcctacg atattggata tgcgtataat
     3121 ccgaatggga aatggacatc gcatcatcaa atgtcaatta atggcaaatt tgacaagatt
     3181 actaaaactg atttacttga atgtgcttat aaaaataata tcaaaaacgc gcaacagatt
     3241 ataggagaag tatgtgatgc cgcatctaaa tggaataaaa tagctcaaga atgtgacatc
     3301 cccaaaacaa taatcaacaa tataatacca aacttacaat taaatttata ataatgcaat
     3361 atgtctattt tgaaacatta ttagcaatac ctaaacgtaa tatgtcaata tagaaatatt
     3421 ataagatatt atcggagttt gtttaagcaa tattagatat ttgtcgcagc actgttgata
     3481 aaatatttct tcttattgtg actaaacaaa caaaaagaag aataaaattg taaatttgca
     3541 aaaaagaaat taaatatgac gatgaaagca aaaccattca ttaaatgggt tggcggaaaa
     3601 acccaactaa ttgaaagcat tgaaaaggct ttgcctgaga attttgctga aattaaaaat
     3661 gttacttata tagagccatt tgttggtgga ggagcaatct tattctggat tttacaacaa
     3721 tacccgaata ttacaaaagc ggttattaat gacataaatc cagatttaac aactgcttat
     3781 aaaacaatta aagaaacacc gaccgaatta gttaaatatc ttcaaataat tcaagatgag
     3841 tatttgccat tggatgaaga aaaacggaaa gaatactatc tgaacaaaag ggaacggttt
     3901 aatacaaaat cactcgaatc tattgagaat actgcattat ttatcttttt aaaccgtact
     3961 tgtttcaatg gcttatatcg tgtaaacagt aaagggcttt tcaatgttcc atttggacga
     4021 tacgctaatc ctaaaatttg cgacaaacaa acaatttttg ctgatagtga actcttgcaa
     4081 aaagtagaaa tattgacagg agattttgag aatacattaa aatatgctac aaataatagt
     4141 ttcttttatt ttgatcctcc ttataaacca ttaagtgata cttcaagttt caattcttat
     4201 gctaaagaag atttcaacga cttagaacaa attcgattag gcaatttttg taaaaaaatt
     4261 gacttacttg gtc
//
LOCUS       DAOPID010000127         4259 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_28151_length_4259_cov_3.967650, whole genome shotgun sequence.
ACCESSION   DAOPID010000127 DAOPID010000000
VERSION     DAOPID010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28151_length_4259_cov_3.967650"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3754)
                     /locus_tag="PK641_04985"
     CDS             complement(<1..3754)
                     /locus_tag="PK641_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55736.1"
                     /translation="MKSKLPLLSLLASALLLLLPSNLAATTYCDTPITSLSGNKSATV
                     SCESLGSNQYRFTLVSDESFTSATNKNVGCNPGPVNIGAILSVQADNKTLTGEFTCTG
                     APTLYVATIFINYSSGEERFDIPLDAEFSVCGSPTPIPPTIGAVTVVSSYNGNAELAF
                     SITGSTPITYTVTCDGNTISSGDYTYSSGKLKINSSYFTVGAHSVNITATNSEGSDSK
                     TGIVVNTSAAPQKCGTVLGGDGSSIDYLFITNPDNSVTITINATANLSTAIVYIGAPG
                     YIMTMNGSIGTYTHPASLIGAQTELSNIYFLYNIGAGQQSTSASPLNYTIGTICYDSE
                     SPQWGSDPSFVAASARSFVLDVNASDNIGVKGFNVKLGTCESVDVQITESVLYDGSIT
                     IPLPAGCPDLPSDAGSAKSATIEAYDAAGNVSISKIVGGIYKTITPPTMVSASCIDPI
                     GTTTMTMAVSATNGTNNVKAYKVVSSSPAYSGTFTPSAGKILLTGLAGNTSYVFDVWA
                     VDDMDVLSENYVTCSANCKTLDPNAPDVPSKLTATPMERTSTGTCSTSIVAGTNFAAS
                     TYAEDTYNLSYSDIVTAEGRLSNLSINSILKESYVNGGFHDVPSSYGEYAIVSNPKVL
                     NGKYPAKSDGKNRFIAGFSTTLNTGNRLNIMTYTFEPLIQSSKFKVSFKYDDMNTAAV
                     GARQLWIVIKEGESVLVDTYLDYSAGSENTYTSAEFTLSGANQKVSVQIDARMLPVET
                     TVAISDLIIYGCVTKSIAQEIDENYVCRGTSVNLRADGFGTGTGFTWYSGSTITGPWT
                     ILPASNTSTITVTPEIVGNNYYRVVNGSDEATTSVLAKVCCDVSGESQQKIVWTEIFG
                     TSLDPNERYTNRAVDSIAVYTFATKENTCGGAEGLGKVCDGYYVVVSNSTAAYAHMCG
                     WPSGKFDHTTSDGTGGYLIVNAGTENKLLYEKEIYPEGGFCSGIWYNFSLYATNIAPS
                     GSSPARFTLEIIEKRAGGVEIELSNFDTGNIEGFSMSQWFNYGTSFTPSDFAEQIVVR
                     IWNTGGSTNGNDLVLDDLSVSICQPKAGIFIGDIADKKKEETEISCGTTVSLSAVLNG
                     YQADFFSSTPYYLWTMNTGSGWTEISGASGIGVDGYSHMTQDGVTTQVKTILAGDRET
                     ALKVLNGEEVGSCSSNAVTDVATVSCKNNCPNPATIIWKSDSGKQNQTVCSGESMISS
                     RYELGGSATDYTIEGLPSYMTHSKTGSIIEISGIPTENINYTITTTG"
     gene            complement(3980..>4259)
                     /locus_tag="PK641_04990"
     CDS             complement(3980..>4259)
                     /locus_tag="PK641_04990"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HPG55737.1"
                     /translation="LSILCMGIVAFGLGTGGGVLLAKLINVFSKEKINPLIGSAGVSA
                     VPMAARVSQVVGQEENPGNFLLMHAMGPNVAGVIGSAVAAGILLSMLG"
BASE COUNT         1367 a          862 c          681 g         1349 t
ORIGIN      
        1 gaccggttgt agtaattgtg taattaatat tttctgttgg aataccacta atttcaatta
       61 tcgatccggt tttactatga gtcatataag atggcaaacc ttctatggta taatctgtag
      121 cagaacctcc taattcatat ctgctagaaa tcatagattc gcctgaacat accgtttgat
      181 tttgtttgcc tgaatctgac ttccatataa tagtagcagg attagggcaa ttattcttac
      241 aagaaacagt agctacatct gtaactgcat ttgaagaaca tgaacctacc tcttcaccat
      301 ttaaaacttt caaagcggtt tcacgatctc cggctaatat agttttaact tgtgtagtta
      361 ctccatcttg tgtcatatgt gaatatccat ctacacctat tccggaagca ccggatattt
      421 cagtccatcc tgatcctgta ttcattgtcc acaaataata aggtgtactt gaaaagaaat
      481 cagcttgata accattaagc actgcagaca aactaactgt tgttccacaa ctaatttctg
      541 tttcttcttt tttcttgtcg gcaatatcac caatgaaaat acctgcctta ggttgacata
      601 ttgaaacaga caaatcatct aaaaccaagt cgttaccatt agtactacca cctgtattcc
      661 aaatacgtac aacaatttgt tcagcaaaat cgcttggggt aaatgatgtt ccgtagttaa
      721 accattgtga catcgagaat ccttcaatat taccggtatc gaaattactt aattctatct
      781 cgacaccacc tgctcttttt tctataattt ctaaagtaaa acgtgcaggt gaagaacctg
      841 atggtgctat attagttgca tacaatgaaa aattatacca tattcctgag caaaaaccac
      901 cttcaggata tatttctttt tcgtacaaca acttattttc agttccggca tttacgataa
      961 gataaccacc ggttccatcg gatgttgtat gatcaaattt acctgatggc catccacaca
     1021 tgtgtgcata agcagctgta gaatttgaca caacaacata gtagccgtca cataccttac
     1081 ctaatccttc agctcctccg catgtattct ctttagttgc aaatgtatac acagctattg
     1141 agtcaactgc tctattggta taacgttcgt ttggatctaa gcttgttccg aaaatttctg
     1201 tccatacaat tttttgttga gattcacctg atacatcaca acaaactttt gctaaaacgc
     1261 ttgtagtagc ctcgtctgaa ccattaacaa cgcgataata gttattacca actatttccg
     1321 gagttacagt tatagttgat gtgtttgatg ccggcaatat agtccaaggt ccggtaatag
     1381 ttgatcctga ataccatgta aaccctgttc ctgttccaaa accatcagct cttaaattaa
     1441 cagaagtacc acgacaaaca taattctcat ctatttcctg agcaattgat tttgtcacac
     1501 atccgtatat aataaggtca gatattgcaa cagttgtttc tacaggcaac attctagcat
     1561 caatctgaac tgaaactttt tgattagctc ctgacaatgt aaattctgca gaagtgtaag
     1621 tattctctga tcctgctgaa taatctaagt atgtatcaac taatacactt tcgccctctt
     1681 ttattacaat ccataactgt cttgcaccga cagcagcagt attcatatca tcatatttga
     1741 atgatacctt aaatttactt gactgaataa gaggttcgaa tgtatatgtc attatattta
     1801 aacgatttcc tgtgtttaat gtggtagaaa aacctgcaat aaaacggttc tttccgtcag
     1861 attttgcagg atacttacca tttaaaactt taggatttga cactattgca tattcaccgt
     1921 atgaactagg cacatcgtgg aatccgccat taacataaga ttctttaaga attgaattta
     1981 tggacagatt actcaaacgt ccttcagcgg ttactatatc cgaatatgat aaattatatg
     2041 tatcttcagc gtatgtagat gcagcaaaat tagttcctgc aacaattgaa gtagaacatg
     2101 taccggttga tgtacgttcc attggtgtag ctgtcaactt agaaggtaca tccggagcat
     2161 tcggatccaa tgttttgcaa ttagccgagc aagtaacata attttctgac aatacatcca
     2221 tatcatcaac agcccaaaca tcaaacacat aagatgtatt acctgcaagt ccggtcaata
     2281 atattttacc ggcacttgga gtaaaagttc ccgaataagc aggtgatgaa gaaacaactt
     2341 tataggcctt aacattgtta gttccatttg ttgcacttac agccattgtc atagttgtgg
     2401 ttcctattgg atcgatacaa cttgctgata ccattgtagg aggtgttatt gttttataaa
     2461 taccacctac aatttttgat atagaaacat tacctgcagc atcataagct tcaatagttg
     2521 ctgattttgc acttcccgca tcacttggaa gatcaggaca tcccgcaggc aatggaatcg
     2581 tgattgatcc atcatataat acagactctg taatttgaac atcaaccgat tcgcatgtac
     2641 ctaattttac attaaatcct tttacaccaa tattatcact agcatttaca tctaaaacaa
     2701 acgagcgtgc tgatgcagca acaaaacttg ggtctgaacc ccattgtggt gattcgctat
     2761 cataacaaat agttcctatt gtataattta atggacttgc tgatgtactc tgttgaccgg
     2821 cacctatatt atatagaaaa tatatatttg acaattctgt ttgagctcca attaatgaag
     2881 ccggatgagt atatgttcca atactaccat tcattgtcat tatatatcca ggagcaccta
     2941 tatatacaat tgcagtactt aaattagcag tagcattgat tgttattgta actgagttat
     3001 cgggatttgt aataaataaa tagtcgatcg atgatccatc tcctcctaat acagttccgc
     3061 atttttgagg tgcagcactt gtgtttacta caataccggt tttagaatct gagccttcac
     3121 tattagttgc tgttatattt acagaatgag ctcctactgt aaaataactg ctattaattt
     3181 ttaatttacc tgatgaataa gtataatcac cagaagatat tgtattacca tcacaagtaa
     3241 ctgtataagt aataggagta gatcctgtta tagaaaatgc aagttcagca ttaccattat
     3301 atgaagagac tacagttaca gcgcctatag taggagggat tggggttggc gaaccgcata
     3361 cagaaaactc tgcatcaaga ggaatgtcaa atctctcctc gccggaacta taattaatga
     3421 aaatagttgc cacataaaga gttggagccc ctgtacaagt aaactcacct gtaagagttt
     3481 tattatcagc ttgaactgaa agaatggcac cgatattcac aggccccgga ttacaaccga
     3541 cgtttttatt agtggcacta gtaaaagact catcactaac taatgtaaaa cgatattgat
     3601 tactacctaa tgactcacaa gatactgtgg cacttttatt tccgctaagt gaagtaatag
     3661 gcgtgtcgca ataggtagtc gccgccaaat ttgacgggag tagtaagagc agagcactag
     3721 ccaataatga caatagaggt aatttgcttt tcattttatt tgatttttaa gattattcaa
     3781 caatttgaaa cgcaaagata ctattaattt ttatttgcaa acgattgacc tatccacttt
     3841 ttattacaca ttaatacatc aatgcaccac aacaaccatt tctttagata acataataca
     3901 ttataaaaca gagttttata tacaaaaatg aggcttgatt ttaaaaatca agcctcaata
     3961 attaatataa tacaaattgt tatcctaaca tacttaacag aattcctgcc gctacagccg
     4021 aaccaattac acctgcaaca ttaggtccca ttgcatgcat aagcaagaaa ttacccggat
     4081 tttcctcttg tccaacaact tgagaaaccc gtgcagccat aggtactgct gaaacaccgg
     4141 cagaacctat tagtggattg attttctctt tactgaacac attaatcaat tttgcaagca
     4201 gcacaccacc gcctgttccc aacccaaatg caacaattcc catacataaa atcgataag
//
LOCUS       DAOPID010000128         4217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_28526_length_4217_cov_4.010332, whole genome shotgun sequence.
ACCESSION   DAOPID010000128 DAOPID010000000
VERSION     DAOPID010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28526_length_4217_cov_4.010332"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PK641_04995"
     CDS             complement(<1..228)
                     /locus_tag="PK641_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPG55738.1"
                     /translation="MISIVIPLYNKAQSIANTIRCIQQQTYQDFEVVVIEGWSTDGST
                     ELIHQLAAQDTRIHVLMQQNRKGVTPARNEGV"
     gene            complement(231..800)
                     /locus_tag="PK641_05000"
     CDS             complement(231..800)
                     /locus_tag="PK641_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPG55739.1"
                     /translation="MKKDYSENIKLYLDKLKKTIDILDLEQWNNFINLLEDACETGKQ
                     IFIMGNGGSAATASHYVCDFNKGISYGKEKRFKMICLNDNVPTMMAYSNDVSYNEVFV
                     EPLKNFYNQGDLVIGISGSGNSKNVVKAMEWANQNNGVTIAITGYNGGVLKQICKHNV
                     HVPINDMQISEDLHYILCHCIMQVLGGYC"
     gene            complement(804..1781)
                     /locus_tag="PK641_05005"
     CDS             complement(804..1781)
                     /locus_tag="PK641_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HPG55740.1"
                     /translation="MIISRTPLRISFAGGGSDLPSFYHHQQGAVLSTSIDKYVYLAVH
                     EFFYPNQTLLKYSKTELVNNVSDIQHPIFRECLSMLNLSGLDIASMADVPAGTGLGSS
                     SSFTVSLLNVLHAYKRHYVSAEYLAKTACEIEIDRLGEPIGKQDQYAAAYGGLNFIRF
                     NGDESVDVEKIIMDPDAKKQLENNLIMIYTGQTRSASDILKNQNKEMSSEAKRNIVHQ
                     MVEIAFALKDVLQQNKIDEFGKILHEGWILKQSLSQGISNQDINDLYEKGIKAGALGG
                     KLLGAGGSGFLLFYCPQEKQYIFRQKMSENKELAFKFDNSGSKIIYIAE"
     gene            complement(1822..3078)
                     /locus_tag="PK641_05010"
     CDS             complement(1822..3078)
                     /locus_tag="PK641_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HPG55741.1"
                     /translation="MQAQVVIIAGGKGTRIASIASDIPKSMIPIAGKPVVEYQIELAK
                     RYGYKEILFMIGHLGDQIKKYFGNGEKWGVNIEYYLEDAPMGTAGALPFVEEMLAETF
                     FVFYGDTIMDFDMTEMMKFHVEHEASATLFLHPNDHPYDSDLVETDFNGIIKKFIFKP
                     HDELLMYRNLVNAALYVMDKKCIDYIPEGKCAMEKELFPRLLDANEKLAAYASPEYIK
                     DMGTPDRWEHVTADVVSGKVKRLNRKYKRPAVFLDRDGVISKEIDLLHKVEQLELIDG
                     AADAISLINKSGYLAIVVTNQPVIARNLCSFEELQNIHNRMETLLGEKHAYLDAIYFC
                     PHHPDKGYPEERVEYKIDCNCRKPKPGMLLKAAEDFNIDMSESFMIGDRPQDIQAGNA
                     ADVKNSILIETNVPNALLNVVKELAL"
     gene            complement(3078..4013)
                     /locus_tag="PK641_05015"
     CDS             complement(3078..4013)
                     /locus_tag="PK641_05015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HPG55742.1"
                     /translation="MMFSIVIPLYNKAKSIANTIQSILGQSFADFELIIVDDGSTDDS
                     VQVVNQFTDKRIRLIQKENGGVCSARNVGIKNSKYEYIALLDADDYWDKDFLLEASKL
                     IEDFPQAALWGINFAEIYEGKLIRELPTGLPKGFRGIVEDYFEMPEKMGRISDLFCSS
                     SVVIRKSIFDHTGMFDERLKYSEDIDMWYRIIANFPVAFYDRYMSFYQYDADNRAMNR
                     KRTLKYFLPYYSDKYLPYKNNSVFYNFIIRWCAVNIKDIYFNDKSQRNDAKVAAKKLD
                     YSVLPSKYKYLFNMPYCIAYILYLITEYRLKLKNK"
     gene            complement(4010..>4217)
                     /locus_tag="PK641_05020"
     CDS             complement(4010..>4217)
                     /locus_tag="PK641_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55743.1"
                     /translation="IFWDRKTDNSAALAKIKELHDSPKLLKEFLAQPRLTPHAAEYVL
                     DTFSTLEHKLKSVIESKAKQRYRQ"
BASE COUNT         1351 a          840 c          586 g         1440 t
ORIGIN      
        1 tacgccttca ttacgcgctg gagtcactcc tttacgattt tgttgcatca gcacatgaat
       61 acgcgtatcc tgcgccgcca attggtgtat cagctcggta ctaccgtcgg ttgaccaacc
      121 ttcgattacc acgacttcaa agtcttgata cgtttgctgt tgaatacagc gaatggtatt
      181 ggcaatactt tgagctttat tatacaatgg aattactatc gaaatcataa ttaacaatag
      241 cctcctaaaa cttgcattat acaatggcac aagatgtagt gtaaatcttc tgatatttgc
      301 atatcattta ttggcacatg tacattgtgt ttgcaaattt gttttaatac accaccatta
      361 taacctgtaa tagcaatcgt aactccatta ttttgattag cccattccat agcctttaca
      421 acatttttag aattacctga gcctgaaatg ccaattacta aatcaccttg attataaaag
      481 ttcttaagcg gttcaacaaa aacttcgtta tatgacacat cattagaata tgccatcatt
      541 gtaggaacat tatcgttcaa acaaatcatt ttaaatcttt tttctttacc ataagaaatg
      601 cccttattaa agtcacatac ataatgcgat gctgtagctg cagagcctcc atttcccata
      661 ataaagatct gtttaccggt ttcgcaggca tcttctaaaa gattaataaa attattccat
      721 tgctcaagat ctaatatgtc tatagtcttt ttaagtttgt ctaaatacaa cttaatattt
      781 tccgagtaat cttttttcat attctattct gctatgtaaa ttattttaga acctgaatta
      841 tcaaacttaa atgctaactc cttattttct gacatttttt gtctgaatat atattgtttt
      901 tcttgtggac aataaaacaa caaaaaacca ctacctcccg cacccaataa tttgccacct
      961 aatgctcctg ccttaatgcc tttttcatac aagtcattaa tatcctgatt tgatattcct
     1021 tgtgacaaag attgcttcaa aatccaacct tcatgtaaga tttttccaaa ttcatctatt
     1081 ttattttgtt gcaaaacatc ctttaaagca aatgctattt ctaccatttg atgaactata
     1141 ttgcgttttg cttcagaact catctcttta ttttgatttt taagaatatc gcttgccgaa
     1201 cgagtttgtc ctgtatatat cattatcaaa ttgttttcta actgcttttt tgcatctgga
     1261 tccataatta ttttttcaac atcaacagat tcatctccat taaaacgaat aaagttcaaa
     1321 cccccataag cagctgcata ttgatcttgt ttaccaattg gctcaccgag tctatctatt
     1381 tctatctcgc aagctgtttt tgccaaatac tcagcagata cataatgacg tttataagca
     1441 tgcaaaacat ttaacaaact aactgtaaat gaactgctag aaccaagacc agtgccggca
     1501 ggtacatctg ccattgatgc aatatccaaa ccagataagt taagcattga caaacattct
     1561 ctaaaaatag gatgctgaat gtcagataca ttattaacca attcggtctt tgaatatttc
     1621 aataaagttt gatttggata aaaaaactca tggacagcca aataaacata tttatctatt
     1681 gaggtgctaa gaactgcacc ttgttggtga tgataaaaac ttggcaaatc agaaccacca
     1741 ccggcaaatg aaatacgtaa aggggtacga gatattatca tatattttat aatcgtaata
     1801 attaatatac aacaagacaa attacaaagc taattcttta acaacattaa gcaaagcatt
     1861 tggcacattt gtttcaatca aaatactgtt tttaacatcc gcagcattac ccgcctgaat
     1921 atcttgaggt ctatctccta tcataaaaga ttcagacata tcaatattaa aatcttcagc
     1981 tgctttcaaa agcattcccg gttttggttt tctgcaatta cagtctattt tatattcaac
     2041 gcgttcttca ggataacctt tatccggatg atgaggacaa aaatatattg catccaagta
     2101 tgcgtgtttt tcacccaaaa gtgtttccat tcgattatga atgttctgca attcttcaaa
     2161 agaacataga ttccgcgcta ttacaggctg atttgtaacg acaattgcta aataacctga
     2221 tttattaatc aaagatattg catctgcagc accatctatt aattctaatt gctcaacttt
     2281 atgcaacaaa tcaatttctt tacttattac cccatctcta tctaaaaaca ctgcaggacg
     2341 tttatatttc ctatttaaac gtttaacctt acctgataca acatcggctg ttacatgttc
     2401 ccatctatca ggtgtaccca tatcttttat atattcggga gatgcataag ccgctaattt
     2461 ctcattagca tccaacaaac gtggaaacag ctctttttcc atggcacatt taccttctgg
     2521 tatatagtca atacattttt tatccatcac gtataaagct gcattaacta gattgcggta
     2581 cataagcaat tcatcgtgag gtttaaatat aaatttcttt ataataccat taaaatctgt
     2641 ttcaaccaaa tccgaatcat aaggatgatc gttaggatgc aaaaacaatg ttgcggatgc
     2701 ttcgtgctct acatgaaatt tcatcatttc ggtcatgtcg aaatccatta tggtatcacc
     2761 ataaaacaca aaaaaagtct ccgccagcat ctcttctaca aaaggcagag caccggccgt
     2821 tcccataggt gcatcttcta aataatactc tatattaaca ccccattttt cgccgtttcc
     2881 aaaatatttt ttgatttgat cacctaagtg accaatcata aaaagaattt ctttataacc
     2941 atatcgtttg gctaattcta tctggtactc aacaacaggc ttacctgcta taggaatcat
     3001 tgattttgga atatctgatg caattgatgc tattcttgtt ccttttccgc ctgctattat
     3061 aacaacttga gcttgcatta tttatttttg agttttaaac gatattctgt aataagatat
     3121 aagatatatg cgatacaata cggcatatta aataagtatt tatattttga cggcaaaacc
     3181 gaataatcta acttttttgc agcaactttt gcatcattgc gctgagactt atcattaaaa
     3241 taaatatctt ttatatttac tgcacaccac ctgataataa aattgtaaaa tacggaatta
     3301 tttttgtatg gtaagtactt atcactataa tatggcaaaa aatattttag agtccttttt
     3361 ctattcattg ctcgattatc ggcatcgtac tgataaaaag acatatacct atcataaaac
     3421 gcgacaggaa aatttgcaat aatccgatac cacatatcaa tatcttcgct gtattttaaa
     3481 cgttcatcaa acattccggt atggtcaaaa attgattttc taattactac agaagacgaa
     3541 caaaacaaat ctgaaatgcg tcccatcttt tccggcatct caaaataatc ttccactatg
     3601 cctctaaaac cttttggtaa acctgtaggc aattcgcgaa tcaattttcc ttcatatatt
     3661 tcagcaaaat taatgcccca taatgctgct tgcggaaagt cttcgattaa tttacttgct
     3721 tccaataaaa aatccttatc ccaataatca tctgcatcta atagagcaat atattcatat
     3781 tttgaatttt taatacctac atttcgtgca gaacaaacgc caccattctc tttttgaatg
     3841 aggcgaatac gtttgtcagt aaattggttc actacctgca cactgtcatc ggtagagcca
     3901 tcgtccacaa taatcaattc aaagtcggca aaagattgac ctagaatact ttgaatggta
     3961 tttgcaatgc tcttagcttt gttgtaaagc ggtatgacaa tggaaaacat cattggcgat
     4021 agcgttgttt agcttttgat tcaatcactg attttaattt atgctctagc gtcgaaaacg
     4081 tatctaatac atattcggca gcgtgtggtg tcaagcgtgg ttgcgctaga aattctttga
     4141 gtaattttgg agagtcatgc aactctttaa tttttgccaa cgcagcacta ttatctgttt
     4201 tacggtccca aaagata
//
LOCUS       DAOPID010000129         4191 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_28758_length_4191_cov_4.526112, whole genome shotgun sequence.
ACCESSION   DAOPID010000129 DAOPID010000000
VERSION     DAOPID010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28758_length_4191_cov_4.526112"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PK641_05025"
     CDS             complement(<1..446)
                     /locus_tag="PK641_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HPG55744.1"
                     /translation="MGFFSFFKKNTPEEKREVLDNGLSKTKDSVFDKLSRAIVGKSKV
                     DDEVLDNLEEALVTSDVGVDTTLRIIERIQSRVARDKYVNTSELDHILREEIIDLLAE
                     NDNSDFADIEFDSSENPYVIMVVGVNGVGKTTTIGKLAYQFKQAGK"
     gene            complement(587..739)
                     /locus_tag="PK641_05030"
     CDS             complement(587..739)
                     /locus_tag="PK641_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007214255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HPG55745.1"
                     /translation="MAKKTVATLQKGEGRAYAKVIKMVKSPKTGAYVFEEEMVLNEKV
                     KDYLAK"
     gene            complement(750..938)
                     /gene="rpmG"
                     /locus_tag="PK641_05035"
     CDS             complement(750..938)
                     /gene="rpmG"
                     /locus_tag="PK641_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HPG55746.1"
                     /translation="MAKKSKGNRVQVILECTEHKASGMAGTSRYITTKNRKNTTERLE
                     LMKYNPILKKMTLHKEIK"
     gene            complement(963..1196)
                     /gene="rpmB"
                     /locus_tag="PK641_05040"
     CDS             complement(963..1196)
                     /gene="rpmB"
                     /locus_tag="PK641_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HPG55747.1"
                     /translation="MSKICQITGKKAAIGNNVSHSKRRTKRKFDINLFTKKFYWVEQD
                     QWISLNISANGLRVINKMGLDAAIKRAAEKGYL"
     gene            complement(1288..2415)
                     /locus_tag="PK641_05045"
     CDS             complement(1288..2415)
                     /locus_tag="PK641_05045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPG55748.1"
                     /translation="MKIIILGTAHPYRGGLAAFNNCLAEQFVKEGHDVTLYTFTLQYP
                     SLLFPGKTQYTNDEAPKNISILRRINSINPFNWISVGREIRKQKPDIVIFAYWMSFMA
                     PCYGVIAQVIKKDPAIKCISLVHNFIPHEPSLLDKILPKYFVKKMDAFVAMSESVATD
                     LTRAVSFNVPVKVTPHPIYNQYGNLKDRNEALSHLRLDHDFRYVLFFGFIRPYKGLDL
                     LLEAMSDSRLNKYSVKLIVAGEFYGNPQPYYDSVKKMNIVEKVEWFNRFIPDAEVSNF
                     FSAGDILVLPYKSATQSGVTQIAYHFEKPMLVTKVGGLSDMVPEGKVGYVVEPNPSSI
                     ADALVDFFEFGDENRFKEGLISEKDKYSWSKMTRTIISIEI"
     gene            2464..3597
                     /locus_tag="PK641_05050"
     CDS             2464..3597
                     /locus_tag="PK641_05050"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPG55749.1"
                     /translation="MKFRIIAIITVVLSLSACSSNKQTKILENASADSSATLKYAKWF
                     SIDYYPDYKCVSVINPWVKNKIINKYYLVNDSSITTPSDGYKIKIPIRKLAVNSCSHL
                     GFLGILKESESVIGICTKERIYDPIILKRCAENKCTDIGDAFAINYEKTLLIQAEAVM
                     VSTFNQTDNHVERIRDAKIPVIDNVEWMESHILGRAEWIKFVAAFYNKEHIADSIFNE
                     VCFNYDNLKSLAKVTENRPNILPGLSFKGTWYMPGGNSFMGRLFFDAGGNYKFSDDTT
                     STSLPISFEVALKYFKNCDIWLGVDANSFEELKAADDRLCMFKAYRTKNVYNNNKRTT
                     PSGGNDFWESALARPDILLKDLIIAMHPELLPGQSCFYIKKLE"
     gene            3617..>4191
                     /locus_tag="PK641_05055"
     CDS             3617..>4191
                     /locus_tag="PK641_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005809813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HPG55750.1"
                     /translation="MTKPAKISISFLLLIIVLFAAFIADILLGSVYIEPKTIIENLLN
                     GTIESDPIINDIILNFRLPKAITAILCGASLATAGLLMQTLFRNPLAGPYVLGISSGA
                     SLGVAILVLAAGSSIIPAFLVFSGWGQVIAAIIGASLILMLVLGTSLKINDTVSLLIV
                     GMMFGSLSGAVVNILQSISNPDALKIYIVWT"
BASE COUNT         1355 a          735 c          655 g         1446 t
ORIGIN      
        1 tttttacctg cttgtttaaa ttgatatgcc aatttaccta tagtagtagt tttacctact
       61 ccatttactc ctacaaccat tattacataa ggattttcag aactgtcaaa ttcaatatct
      121 gcaaaatcag aattatcatt ttctgctaat aaatcgataa tttcttctct taatatatgg
      181 tcaagttctg aagtgtttac atatttatct cttgcaacac gtgactgaat tctctcaata
      241 attcgtaaag ttgtatcaac tccaacatct gaagttacaa gagcctcctc caaattatct
      301 aaaacctcat catctacctt tgatttacct actattgctc ttgacaactt gtcaaaaacg
      361 ctatctttgg ttttagaaag accattatca agaacttccc gtttttcttc gggggtattt
      421 tttttaaaaa agctgaaaaa tcccataata ctagtattaa taaatatgcg cgataaagat
      481 tatttttccc aaacacctca tccacgcaaa gatactacat ttaatgcaaa caaaaaacct
      541 ttcggaatcc cgaaaggttt tttaaaatat catgtaaaga tactatttat tttgccaaat
      601 agtctttcac tttctcatta agtaccatct cttcttcgaa gacataagca cccgttttag
      661 gcgattttac cattttgatg acttttgcat atgcacgtcc ttcgcctttt tgcaatgttg
      721 caactgtttt ctttgccata tcttatatct tatttaattt ctttgtgcag agtcatcttt
      781 ttcaagatgg gattgtattt catcaactcc aagcgttcgg tagtattttt acgattctta
      841 gtcgtaatat aacgtgatgt accggccatt ccacttgctt tgtgttctgt acactctaag
      901 attacttgta ctctatttcc ttttgacttt tttgccatga cttaatctcc tctactttac
      961 gattatagat aacctttttc agcagcacgc ttaatagctg cgtccaagcc catcttgtta
     1021 ataacacgca gaccattggc tgaaatattc aggctgatcc attgatcttg ttctacccaa
     1081 tagaatttct tggtaaaaag atttatatcg aatttacgtt ttgtacgtct cttcgagtgg
     1141 gaaacattgt tgccgatagc ggctttttta ccggtaattt gacaaatttt tgacatcgct
     1201 gtatcttttt tttgtatatc ttcaaaagag agtgcaaagg aaatactttt tttttaatta
     1261 cacaaatgtt tgatgataaa aaaacaatta aatttcaatt gatattattg ttcttgtcat
     1321 tttgctccaa gagtatttat ctttctccga aattagtcct tctttaaatc tattttcatc
     1381 tccaaattca aagaaatcga ccaatgcgtc tgcaatagaa ctaggattcg gttcaactac
     1441 atatccaact tttccttccg gaaccatatc tgaaagacct cctactttag ttactaacat
     1501 aggcttttca aaatgatatg ctatttgagt tactccactt tgagttgccg atttataagg
     1561 taaaacaagt atgtcaccgg cactaaagaa gtttgaaacc tcagcatcag gtataaatct
     1621 attaaaccac tcgacctttt caacaatatt catttttttt acgctatcat aatatggttg
     1681 aggatttcca taaaattcgc ctgctacaat taatttaacc gaatatttat ttaatcttga
     1741 atcagacatc gcttctaaca ataagtctaa tcctttataa ggacgaataa aaccgaagaa
     1801 taaaacatat ctaaaatcat gatccaatcg taaatgggac aatgcttcat ttctatcttt
     1861 taaattgcca tactggttat atatgggatg tggcgtaact tttacaggaa cattaaatga
     1921 tacagcccta gttagatctg ttgcaacaga ctcagacatt gcaacaaaag catccatttt
     1981 ttttacaaaa tattttggta gtattttatc taacaaagat ggttcgtgcg gtataaaatt
     2041 atgcaccaat gatatacact taattgccgg atctttttta ataacttggg ctataactcc
     2101 gtaacaagga gccataaatg acatccaata tgcgaatatc acaatatcgg gcttttgctt
     2161 tctaatttct ctgccaacag atatccaatt gaatggatta attgagttaa tgcgtcttaa
     2221 tatagaaata ttttttggcg cctcatcgtt agtatactga gttttaccgg gaaacaacaa
     2281 tgagggatac tgcaaagtaa atgtatataa tgtcacatca tgaccttcct ttacaaattg
     2341 ctctgcaaga caattattaa atgctgccag acctcctcta tatggatgag ctgtaccgag
     2401 tataattatt ttcatagtgg caaaaataca aatttttcat gaagatttga ttacctttgc
     2461 aaaatgaaat ttcgtattat tgctattatt acagttgtat taagtttgtc cgcatgttca
     2521 tcaaacaaac aaactaaaat attagagaat gcaagcgcag actcttccgc tactctaaaa
     2581 tatgcaaaat ggttttcaat cgactattat cctgattaca aatgtgttag tgtaataaat
     2641 ccatgggtca aaaataaaat cataaacaag tattatttgg ttaacgactc atccattaca
     2701 actcctagtg atggatataa gattaagatt ccgatacgaa aactagcagt aaattcttgt
     2761 agccatttag gattcttagg tatattaaaa gaatctgaaa gtgtaattgg catctgcaca
     2821 aaagagcgta tatacgaccc cataatttta aaacgttgtg ctgaaaataa atgcacagat
     2881 ataggagatg catttgccat taattacgaa aaaacgcttt taatacaagc tgaagctgta
     2941 atggtaagta cttttaacca aacagataat catgtagagc gtattcgtga tgcaaaaatt
     3001 ccggttattg acaatgtaga gtggatggag tctcatatat taggaagagc tgaatggatt
     3061 aaatttgttg cggcatttta caacaaagaa catattgctg atagtatttt taatgaagtt
     3121 tgcttcaatt atgacaatct aaaatcacta gcaaaagtta cagaaaatcg acctaatata
     3181 ctacccggtc tatcttttaa aggaacctgg tatatgcccg gaggtaatag ttttatgggt
     3241 aggttatttt ttgatgcagg aggtaattat aaattttcgg acgatacaac atctacaagt
     3301 ttaccaataa gttttgaggt ggcattaaaa tacttcaaaa actgtgatat atggttggga
     3361 gttgatgcca atagttttga agagttaaaa gctgcagacg acagattatg tatgttcaaa
     3421 gcatatcgca ctaaaaatgt atataataat aataaacgca ccaccccaag cggcggtaat
     3481 gatttttggg aaagtgcatt agctagacct gacattctat taaaagactt aataattgcg
     3541 atgcatccgg aattgttacc cggtcaatct tgcttttaca taaaaaaatt agagtaatta
     3601 ttgtatattt gcaagtatga caaaaccagc taaaataagt attagttttt tgctccttat
     3661 aatagtttta tttgccgcat ttattgctga catattactt gggagtgtct acattgaacc
     3721 gaaaaccatt attgaaaact tattaaatgg aacaattgag tcagacccta tcataaatga
     3781 tattattctt aatttcagac ttcctaaagc tattacagct atactttgcg gtgcaagttt
     3841 agctactgcc ggattactca tgcaaacttt gtttagaaac ccactagccg gtccttatgt
     3901 tttaggtatt agttccggtg caagtttagg cgttgctata ttagtgttgg cagcaggtag
     3961 ttcaataatt ccggcatttc ttgtatttag cggatggggt caagtaattg ctgccataat
     4021 aggtgcatca ctcatattaa tgctagtttt aggaacttct ttaaaaataa atgacacagt
     4081 atcactactt atagtaggta tgatgtttgg gagtttatca ggtgcagttg taaacatact
     4141 acaaagcata agtaatcctg atgctctaaa aatatacata gtttggacaa t
//
LOCUS       DAOPID010000130         4190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_28772_length_4190_cov_3.965417, whole genome shotgun sequence.
ACCESSION   DAOPID010000130 DAOPID010000000
VERSION     DAOPID010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28772_length_4190_cov_3.965417"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..1370)
                     /gene="metK"
                     /locus_tag="PK641_05060"
     CDS             complement(117..1370)
                     /gene="metK"
                     /locus_tag="PK641_05060"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HPG55751.1"
                     /translation="MAYFFSSESVSEGHPDKIADQISDALLDNFLVQDPNSKVACETL
                     VTTGQVILAGEVKTDAYVDVQTVVRHVIKRIGYTKSEYKFEAESCGVLSAIHEQSDDI
                     NRGVERENPYDQGAGDQGMMFGYACAETDNLMPIALDLSHALLLELAAIRHEGKEMTY
                     LRPDAKSQVTVEYSDDNKPVRIDTIVLSTQHDEFLNDDAKMLAVIEKDVREILIPRVM
                     SKDSKYKALNKDDYKLFVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFSG
                     KDPSKVDRSAAYAARHVAKNMVAAGLADEVLVQVAYAIGVAKPINLYVNTYNTSKVSL
                     SDGEIASKIETLFDMRPKAIEDRLKLRNPIYEETASYGHVGRIPTVVEKTFTSSDGRF
                     IKRKVELFTWEKTDMVDAIKKAFGL"
     gene            complement(1549..2466)
                     /gene="queG"
                     /locus_tag="PK641_05065"
     CDS             complement(1549..2466)
                     /gene="queG"
                     /locus_tag="PK641_05065"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HPG55752.1"
                     /translation="MDASALTQKIKETAIQVGFDAVGITKAELLEEDRAYLDSWLSAG
                     YAGSMNYMHNYFEKRVDVSKLIDGAKSVIVLLYNYMPERLQSASAPKVAKYAYGNDYH
                     HIIKQKLEQVVSQIPEIKLDEQQCFCDSAPLLEKRLAVRAGLGWIGKNTTLINKRFGS
                     YCFISEIVTDKELVYDKPDLSTHCGNCKRCIDECATNALYKEYQMDARKCLSYITVES
                     KDDRSQDEALRSTHTLFGCDKCQSVCPWNLRFAHSSNHEELKALDFLNWTNEDWLNLD
                     KSKFNRIFRDSAMQRAGYEKIMTILNCLM"
     gene            complement(2471..3298)
                     /locus_tag="PK641_05070"
     CDS             complement(2471..3298)
                     /locus_tag="PK641_05070"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPG55753.1"
                     /translation="MKINPKIILLTGASAGIGLAVSELLMWQGHKVYAASRRLVADRQ
                     DIKSGGCICGVCMDVTNEESIRKAVNTILSKESHLDAVICNAGNGIAGAIEDTSDEEA
                     RFQMETNFFGAVNTIRVCLPIFRQQKYGRIIATDSVAGIVPIPFQAFYSASKAALLIY
                     MQALAMEVKEFGIECGCVLPGDTKTDFTASRCFTKASQLDSSCYKDKMKKAVGIMEHD
                     EQNGMSPAQIADAVSCQINGSSVKPILVPGLSYKFICWLMGIIPASLRISIIRKIYS"
     gene            3487..>4190
                     /locus_tag="PK641_05075"
     CDS             3487..>4190
                     /locus_tag="PK641_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HPG55754.1"
                     /translation="MDIIIKEMRTRSELKKFTQFGIELYKNNPYFCPPIIIDEINTLS
                     NDTNPAFEVCESVYYIAFQDKKPVGRIAGIINSAANNAWQVKKLRFGWFDFINNLEVS
                     KALLDAVVEWGRKKGMTQMNGPVGFTDFDHQGLLLEGFEHLSPMASLYNYPYYEQHYD
                     AYGLKKDADWIEIRSGLIHEMPERVDRLCKAVEQRYNVKIEYIHSKKELIKRFGYTFF
                     DVIDSAYSPLYNYSPLT"
BASE COUNT         1353 a          872 c          637 g         1328 t
ORIGIN      
        1 gatttagtca aggtatctaa gaatcgttat ccactacact acctaaagaa ataataatat
       61 aaaaacaggg aacatcatta cgacatcccc tactcattat ttcatatagg ctgaatttat
      121 aaaccgaaag ccttttttat tgcgtcaacc atatccgttt tttcccatgt aaacaactct
      181 actttgcgtt tgatgaaacg accgtcagaa cttgtaaatg ttttttcaac tactgtcgga
      241 attctaccaa cgtgaccata agaagcagtt tcctcgtaaa taggattacg caatttaaga
      301 cgatcttcaa tagccttagg gcgcatatca aataaagtct caattttaga cgctatctcg
      361 ccatcgctta aagaaacttt cgatgtatta tatgtattta cataaagatt aataggcttt
      421 gccactccta tagcatacgc aacttgaaca agcacctcat cagccaagcc ggctgcaacc
      481 atattttttg ctacatgacg tgcagcataa gctgcagacc tatcaacctt tgaaggatcc
      541 tttcccgaaa aagcaccacc gccatgagca cctttgccgc cataagtatc cacaattatt
      601 tttctgccgg ttaagcctgt atctccatga ggacctccaa taacgaattt tccggttgga
      661 tttacaaata acttataatc gtccttgttt aatgccttgt attttgaatc tttagacatt
      721 acacgaggta tcaaaatttc tcgaacatcc ttttcaataa cagcaagcat ctttgcatca
      781 tcattcaaaa attcatcgtg ctgagttgaa agaacaatag tatcaatcct caccggctta
      841 ttgtcatcac tatactctac ggttacttga gattttgcat caggtcgtaa atatgtcatt
      901 tccttaccct catggcgtat agcagctaat tctaaaagca aagcatgaga taaatcaaga
      961 gctataggca tcaaattatc tgtttcggca caagcatagc caaacatcat accctgatct
     1021 ccggcaccct gatcgtacgg attttcgcgc tcaactccgc gatttatatc atcagattgc
     1081 tcgtgaattg cagacaaaac accacaagac tcagcttcaa atttatattc gctttttgta
     1141 taacctatac gttttataac atggcgtacc acagtttgca catcgacata agcatcggtc
     1201 ttaacctctc cggccaaaat cacttgacct gtagttacca aagtttcgca agccaccttt
     1261 gaattagggt cttgtactaa gaaattatca agcaaagcat ccgaaatttg gtcagctatc
     1321 ttatcaggat gaccttcaga gactgattca gaagaaaaaa aataagccat aacaattaaa
     1381 ttaatttaca aattttgttg tatatggatt tgaattaacc gaacggcaaa aaagaagtcg
     1441 tttttagcat ttttttgcgt ggttgcaagc tagttcaaat ccatccacga ttacggctac
     1501 aaagttatga ttaattttta tactaacaca tatctaaata gataaaattt acataagaca
     1561 atttaatata gtcataatct tctcataacc tgcacgttgc atagccgagt ccctaaatat
     1621 tctgttaaac ttcgatttat ccagattcaa ccaatcttca ttagtccaat ttaaaaaatc
     1681 aagtgctttc agttcttcat gattcgaact atgagcaaaa cgtaaattcc acggacacac
     1741 tgactgacat ttatcgcaac caaatagcgt atgagtagag cgtaaagcct catcttgact
     1801 tctatcatcc ttactctcta ctgttatata cgacaaacat tttctggcat ccatttgata
     1861 ttccttgtac aaagcatttg tagcacattc gtctatgcaa cgcttgcaat tgccgcaatg
     1921 agttgataaa tcaggcttgt cgtacactaa ttctttatcg gtaacaatct cagaaataaa
     1981 gcaatagctg ccgaatcgtt tattaataag agtagtattt ttaccaatcc aacctaaacc
     2041 ggctcgcact gccaaacgtt tttcaagcaa tggcgcagaa tcgcaaaaac attgctgctc
     2101 atccaactta atttcaggga tttgagaaac gacttgctct aacttttgtt ttataatatg
     2161 gtgataatca ttgccgtatg catatttagc aaccttaggc gcagaagccg attgcaatct
     2221 ttcgggcata taattatata ataaaactat aactgactta gcaccatcta ttaattttga
     2281 cacatcaact cttttctcaa aataattgtg catgtaattc attgaacctg cataacctgc
     2341 cgacaaccaa gaatcgagat aggctctatc ctcttccaat aattcagctt tagttatgcc
     2401 aacagcatca aaaccgactt gtattgcagt ttctttgatt ttttgagtta aggctgatgc
     2461 atccatatct ttatgaatat atttttctga ttatgcttat acgtaatgat gccggtatta
     2521 tacccattaa ccaacaaata aatttatatg ataatccggg cactaatatc ggcttaacac
     2581 tacttccgtt tatttgacac gaaactgcat ctgctatttg agccggcgac ataccattct
     2641 gttcatcatg ttccattatt ccaacagctt ttttcatttt atccttataa catgatgaat
     2701 ctagttgtga tgcttttgta aaacatcttg aagctgtaaa atctgttttt gtatctcccg
     2761 gcaaaacgca accacactct atgccaaact ccttaacctc cattgcaagt gcttgcatat
     2821 atattaataa tgcagcttta cttgccgaat aaaatgcctg gaatggtatc ggaactattc
     2881 ctgcaacaga gtctgttgca atgatgcgtc catatttttg ctgcctaaag attggtaaac
     2941 atacacgaat agtattcacc gcaccaaaga aatttgtttc catttgaaat ctagcctcct
     3001 catcagaagt atcttcaatg gcccctgcaa ttccattacc tgcattacaa ataacagcat
     3061 ccaaatgaga ctctttacta agtattgtat taacagcttt gcgtattgat tcttcattgg
     3121 ttacatccat acaaaccccg cagatacaac ctccggactt aatatcttgt cggtctgcaa
     3181 ctaaacgtct agaagctgca tatactttat gtccttgcca cattagcaat tcagaaacgg
     3241 ccaaacctat acctgcactg gctcctgtca ataatattat ttttggattg attttcatat
     3301 tttgtcttct gtttttacgt gttaatataa agcatcaaaa gtacaacttt aaaccaacat
     3361 atcaaactct attgctctgt cgaaaatacg tttcatatta ttttgattta aatacaattt
     3421 aaacagatgt tttacgttat tagtaatata ttaaaagcag ctaacaaagc aaaacacact
     3481 aactctatgg atataattat aaaagagatg cggacacgct ccgaactcaa aaaattcaca
     3541 caatttggta tcgaactata taaaaacaat ccgtattttt gccctcctat aattattgac
     3601 gaaattaata ctttaagtaa tgatacaaat cctgcttttg aagtatgtga atctgtttac
     3661 tatattgctt tccaagacaa gaaaccagtt gggcgtattg caggtattat taattctgca
     3721 gccaacaatg catggcaagt aaagaaactt agattcggtt ggttcgactt tattaataat
     3781 cttgaagtat ctaaggctct actcgacgct gtagtagaat ggggcagaaa aaaaggtatg
     3841 actcaaatga acggacctgt aggttttaca gattttgacc atcaaggact tctattagaa
     3901 ggatttgagc atttatcacc aatggcatca ctatataatt atccatacta cgaacaacat
     3961 tatgatgcgt atggcttaaa gaaagatgca gattggatag agattcgttc gggattaata
     4021 cacgagatgc cagaacgagt tgatagacta tgcaaagcag tagaacagag atacaatgta
     4081 aaaattgaat atattcattc taagaaagaa ttgattaaac gttttggata tacatttttt
     4141 gatgttattg actctgcata ttcaccactt tacaattatt cgcccttaac
//
LOCUS       DAOPID010000131         4184 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_28831_length_4184_cov_5.248729, whole genome shotgun sequence.
ACCESSION   DAOPID010000131 DAOPID010000000
VERSION     DAOPID010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28831_length_4184_cov_5.248729"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PK641_05080"
     CDS             <1..473
                     /locus_tag="PK641_05080"
                     /inference="COORDINATES: protein motif:HMM:NF021143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HPG55755.1"
                     /translation="DYSDNLTETQREKLGLLYNIAAAGGFTEFEAMTKTGGFGTQRQG
                     ICPNGFHLPTTEEWDVLAEACGGVAISGKKLKTTTGWYEYDDGTDYYGFAALPAGFAY
                     GSNVYNVGEIVQFWSSNADNDDYLYYAYLYYNDDELFRGSRQDGALSVRCIRNN"
     gene            664..990
                     /locus_tag="PK641_05085"
                     /pseudo
     CDS             664..990
                     /locus_tag="PK641_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646811.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            1236..1985
                     /locus_tag="PK641_05090"
     CDS             1236..1985
                     /locus_tag="PK641_05090"
                     /inference="COORDINATES: protein motif:HMM:NF039745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPG55756.1"
                     /translation="MKKITKSVMLLLASMLFLSSSAYAGGIVIDPAQSYTLSYTISTT
                     TDLPRVYFKLFKTGDDNSFLEIPAQAIDAGTNIVKTNAMIPVGNLTEITRLLFDFGGN
                     PANSEIAITNVELYKTGESENLFTNIVPGERIEYFAPNWAQSANYTYVLDNGAELVLG
                     DATYEAWQAQFGFEFQIATSVDETENSNVQVYSNDGSIVVNADAEQLISIYDVAGKLV
                     VSETASSFNYSVNAGIYIVKVGDKTTKITVK"
     gene            complement(2168..2656)
                     /locus_tag="PK641_05095"
     CDS             complement(2168..2656)
                     /locus_tag="PK641_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008624528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4494 domain-containing protein"
                     /protein_id="HPG55757.1"
                     /translation="MQRYFECKIRYEKQITDTNDKDCGKVKKITETYLVDALSYTEAE
                     ARIVKEMAQYIKGEYSITGIKQAKYAEIFYKEDDLCENWYACKIRITLIDESRGAEKK
                     VPQSLLVRAEGVDDAWKRLMEGMKSTMSDFEITAIGLSPILDYFRYTPDAEITRKIEL
                     TD"
     gene            2794..>4184
                     /locus_tag="PK641_05100"
     CDS             2794..>4184
                     /locus_tag="PK641_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HPG55758.1"
                     /translation="MDLQEEIGRRRTFAIISHPDAGKTTLTEKLLLFGGAIHVAGAVK
                     SNKIKKTATSDWMEIEKQRGISVATSVMGFEYRDYKINILDTPGHQDFAEDTYRTLTA
                     VDSVIIVIDVAKGVETQTRKLMQVCRMRKTPVMVFINKMDREGKDPFDLLDEVESELG
                     IKVRPLSWPINMGFSFKGVYNIYKEDLNLYTPNKQTVSETIAFKDLNSKELEDYIGDK
                     DAAKLREDLELISGVYSPFDVKSYLNGNLAPVFFGSALNTFGVKELLDCFVEIAPSPR
                     PIETEERIVDPLENNFSGFIFKIHANMDPNHRSCIAFVKVCSGKFERNVNYKHIRFGK
                     MMKFASPTAFMAQKKETVDEAYAGDIVGLPDNGNFKIGDSLTSGEDIHFKGLPSFSPE
                     MFMYIENADPMKQKQLQKGIDQLMDEGVAQLFTNQFNGRKIIGTVGQLQFEVIQYRLL
                     HEYNAQCRWEPIR"
BASE COUNT         1365 a          656 c          784 g         1379 t
ORIGIN      
        1 cggattattc agataattta actgaaacgc agcgagaaaa acttggcttg ctttataata
       61 tcgctgctgc cggaggtttt acagagtttg aagcaatgac taaaacaggt ggttttggta
      121 ctcaacgtca aggtatttgt cctaatggtt ttcacttacc aacaactgaa gagtgggatg
      181 ttttggctga ggcttgtggt ggtgtggcaa tctcgggtaa aaaactcaaa actactacag
      241 gttggtatga atatgatgat ggcacagatt attacggctt tgctgctctt cctgccggct
      301 ttgcctatgg tagcaatgtg tacaacgtgg gtgaaattgt ccaattttgg agttctaacg
      361 ccgacaatga tgactactta tactacgcgt acttgtacta caacgatgac gaattgttcc
      421 gcggcagccg tcaggatggc gcactaagtg tacgatgtat taggaataat taaagatgta
      481 aattttataa gcgtagcaat aaaatttatt acgcttaaaa atttgccttt aggagtttgc
      541 aaacctacac agaagcctcg ccatcttcaa aaagtatatt tatcggctca aaaacacccc
      601 taattttggc aattatcggc tcaaagtgag ctgataatat tcacagacga cgagtatatt
      661 cttcttaata atgcgcagaa agtatttaga tctcatgtgg ataatctgac agaaactgat
      721 tacagcaaag ataatgtttt tgataaagct attttggcat tattgttact ctcttatata
      781 aaagcgtttt cagatggaaa taaacgaact acacgtatta ctagtaatgc catattaatt
      841 gccaatggat attgtccaat ttcatttaga agggttgatt cgatagatta caaaaaggca
      901 atgctgatgt tttacgagca aaacagcata tccgctttta aacaaatttt tattgaacaa
      961 tttgaatttg cagtaaaaac ttacttttag caaatttaaa tataaaggtc ggcactgcgg
     1021 cctttgtgtt ttataatacc tattttaaat taacttagta tttgcttaaa taattgtaaa
     1081 tcaaaccaac aaaaccacct aaaaatttgc caatttattt ctgcaataaa tttctattgt
     1141 taaattagtt caaacgtttg ctattgtaaa aatagttaaa tatctttgca tgattatttt
     1201 aattacaaat aagtataaat ttaaatttta cgacgatgaa aaagattact aaatcagtta
     1261 tgttactttt ggcaagtatg ttattcttgt caagttctgc ttatgcaggc ggaatagtta
     1321 ttgatcctgc tcaaagctac acactttcgt acactatttc tactacaaca gatttgcccc
     1381 gtgtatattt taaattgttt aaaacaggtg atgataacag tttccttgaa atacctgcac
     1441 aggctataga tgccggtacg aatattgtta agactaatgc aatgattcct gtaggtaatt
     1501 tgacagagat tacacgtttg ttgttcgatt ttggtggtaa tcctgctaat tctgagatcg
     1561 ccattactaa tgttgaatta tataaaacag gtgaatcgga aaatttgttt acaaacattg
     1621 ttccgggcga aagaattgaa tattttgcac cgaattgggc tcaatcagca aattatactt
     1681 acgtgttaga taacggtgca gaattggttc taggtgatgc tacctatgaa gcttggcaag
     1741 ctcaattcgg atttgaattt caaattgcaa catctgtaga tgaaactgaa aatagcaatg
     1801 ttcaagtgta ttcaaatgat ggttctatag ttgttaatgc cgatgcagaa caattgattt
     1861 ctatttacga cgttgcaggt aaacttgttg tttctgaaac agcatcttca tttaactact
     1921 cagttaatgc aggtatatat atcgtaaaag taggagataa aactacaaaa ataactgtaa
     1981 aataatcagg attataacac agtttatata ataaaaagaa atggaggcat ttatgtctcc
     2041 atttcttttt attatattta ggaactattt gtttatatgt gcaccagaaa cacctccgat
     2101 tcagtatgcc atagcaacaa ttgtaagtcc aaaggctaat gcgatatact tttttataaa
     2161 ttaaaattta atcagtgagt tctatctttc tagttatttc tgcatcagga gtgtatctga
     2221 aataatccaa aatcggactt aatcctattg cagtaatttc gaagtctgac attgtgcttt
     2281 tcattccttc cattaagcgt ttccatgcat catcaacacc ttctgcacgg acaagtaatg
     2341 actgtggaac ttttttctcg gctcctctgc tttcgtctat tagagtgatt cttattttac
     2401 atgcatacca attttcacat aggtcatcct ctttgtaaaa aatttctgcg tatttagctt
     2461 gctttatacc tgtaatacta tattctcctt ttatgtattg tgccatttct ttgactatac
     2521 gtgcttctgc ttcagtgtag ctgagtgcat caaccaaata tgtttctgtt atttttttta
     2581 ctttaccaca gtccttgtcg tttgtatccg taatttgttt ctcgtaacgg attttacatt
     2641 caaaatagcg ttgcatgttt attatattta ttgtttaagg aaacaaaagt aactattttt
     2701 tttacaacca aggtggcgta atttaaaata tttggctcgg taataatcgg atttcaacta
     2761 aatagaatta cctttgcgct gcaaattaga attatggatt tacaagaaga gattggacgt
     2821 aggcggactt tcgccataat atcgcatcct gatgcaggaa aaactacact gacagaaaaa
     2881 ttgctattgt ttggaggggc aatacatgtt gcaggtgctg taaaatcaaa taagattaaa
     2941 aagacagcta catccgactg gatggaaata gaaaagcaac gtggtatttc tgttgcaaca
     3001 tcagtaatgg gttttgaata tagagattat aagataaaca tcttggatac tccaggacac
     3061 caagattttg cagaagacac atacagaacc cttacggcag tagatagtgt aattattgta
     3121 atagatgtag caaaaggtgt tgaaactcaa acacgtaagc ttatgcaagt gtgcaggatg
     3181 cgtaaaactc cggtaatggt atttataaac aagatggacc gcgaaggaaa agatcctttt
     3241 gatttgcttg atgaggttga atcagaatta ggaataaaag tacgtcctct tagctggcct
     3301 ataaatatgg gattttcttt taagggcgtt tacaatatat ataaagagga tttaaatcta
     3361 tatacgccta ataaacagac tgtttccgaa acaattgctt ttaaagattt aaacagcaaa
     3421 gaattggaag actatatagg cgataaagat gcagctaaat taagagaaga tttggaattg
     3481 atttcaggag tgtattcgcc gtttgatgtt aaatcatact taaatggcaa tttggctcct
     3541 gtgttttttg gtagtgcttt aaatacattt ggagtaaaag aattacttga ttgttttgtc
     3601 gagattgcac catctcctcg tccgatagaa acagaagaaa gaattgtaga tccgttggaa
     3661 aacaacttca gcggatttat attcaagatt cacgcaaata tggatcctaa tcacagaagt
     3721 tgtatcgcat ttgtcaaagt ctgttccgga aaatttgaaa gaaatgtaaa ttacaagcat
     3781 attcgctttg gcaaaatgat gaaatttgca tcgccaacag catttatggc gcaaaagaaa
     3841 gaaactgtgg atgaagcgta tgcaggtgat attgttggtt tgcctgacaa tggtaacttt
     3901 aaaattggtg atagtctgac ttcaggtgag gatatacatt ttaaaggatt gccaagtttc
     3961 tctcctgaaa tgtttatgta cattgaaaat gctgacccga tgaaacaaaa acaattgcaa
     4021 aaaggaatcg atcagctaat ggatgagggt gtagcgcaat tatttacgaa tcagtttaat
     4081 ggtagaaaaa ttataggtac ggtaggacaa ttacaatttg aagtaattca atatcgttta
     4141 ctacatgagt ataacgcaca atgtcgttgg gagcctatac gatt
//
LOCUS       DAOPID010000132         4174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_28932_length_4174_cov_3.474873, whole genome shotgun sequence.
ACCESSION   DAOPID010000132 DAOPID010000000
VERSION     DAOPID010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28932_length_4174_cov_3.474873"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1790
                     /locus_tag="PK641_05105"
     CDS             <1..1790
                     /locus_tag="PK641_05105"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPG55759.1"
                     /translation="LGWIVTIVVCTSTLIAISLTPMLCSKLLKSKKVHIDKNGNLIED
                     EEKVDWYQKHVVGFLNKVDTWYAKALHVCLTHKTITIISVVALFAISLVPVFMGAIGT
                     DFMNQSDNGRLTVKIELQRGTRVEETSITARQLEARFATLVPEMRMITTTAGSNDDAG
                     ISALFTSTTNNKIQMTVVCSKKYERKRTIFEIAEVLRKELDRYPEIVDYQCEVSSGMG
                     GGRASTVNIEIYGYDFDTTTNFAEAVKQRIRNNVTGARDIDISRDEDRAELKIVVDKE
                     KIASHGLSSSAISMYVRNRVNGYTAGYLKEDGDEYDIRVRLKEENRNSISDIENMTIP
                     TAKGNLIKLNEIASVQEYWSPPEIERKSRQRIVTVAVTPYETSLGELATAIQKEVDKM
                     DMPAGITIRYGGDYEDQQETFGDLGMLLALIIMLVYIVMASQFESFAKPFIIMMSVPF
                     AITGVIFALLITNTSLDMIGALGVVMLVGIVVKNGIVLVDYTNLMRDRGYELNEAIEL
                     SGASRLRPVLMTALTTVLGMVPMALSSGEGSEMWQPMGIVVIGGLLVSTIITLIVVPV
                     LYAIMSKHGERNKEEKARKEFIFMQLSDK"
     gene            1797..2090
                     /locus_tag="PK641_05110"
     CDS             1797..2090
                     /locus_tag="PK641_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007758092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55760.1"
                     /translation="MKSVFIVFNQANTERVEYMLDQLKVSGFTFFEQVQGRGTNGGEP
                     RRGTHTWPEMNSAVMAVVEDDQVPELLSTVKKLDNRNKEVGVRAFVWNIEQTV"
     gene            2338..3984
                     /locus_tag="PK641_05115"
     CDS             2338..3984
                     /locus_tag="PK641_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55761.1"
                     /translation="MRKTKFSAASALLLLLFLGLAGVTSAQQLPNSNFEAWGNSFDGN
                     PQPSNWTGSNVTQVGMKFTLVYQSTDAHTGSYSVKMADKEVGAMGITEISPSWLTLGT
                     PWSYLEGLSTSSATAGTDGGISFAYRPDTMAVWIKRQSNGLEDMNLVYYSWRGQSKGD
                     KYLNKGNGCTSTTHYDEESDIRQSTDKNSCGTSVQATQVAEGLLRTKTVYNNWTLVKV
                     PITYYNDQIPEKMNIILSASNYPNFRANSGLYDGNSLLVDDISLIYSSKIHEIRLNGI
                     ALPGFKTNTTEYTYEIPKGSSIPTITCYRSGRLLSGSEISVNYAAAGTPTQITVNAGD
                     GSSTTTYKVTFVYTRSQNCKLNGISVNGTPISGFNSSVTNYTVALPFGTTEFPTITYT
                     KADDAQTVTIEPCAIPGTARVVVTAENPDYSSTYLINFTVGELSDTTLEDILVNGVSI
                     TGYAPTKTIYSVNVPVGTTEAPVLSYKSAYADGLQTIVVSQKGLSETSTITVSAPGAS
                     TSRIYKISYNITESSYSYLQDIKLDGVILTDFNPSTLTYT"
BASE COUNT         1387 a          648 c          835 g         1304 t
ORIGIN      
        1 aattaggatg gattgttaca atagttgtat gtacatcaac tcttatagca atttcattaa
       61 ctcctatgtt atgctcaaaa cttcttaagt caaaaaaagt acatattgat aaaaatggta
      121 atttgataga agatgaagag aaagtagact ggtatcaaaa gcatgtagtc ggatttttga
      181 ataaagtaga tacatggtat gctaaggcat tgcatgtttg tcttactcat aaaacgatta
      241 caatcatctc agtagtagca ttatttgcta tatccttagt acctgtattt atgggtgcta
      301 taggaacaga ctttatgaac caaagtgata atggacggtt aacagttaag attgaattac
      361 aacgcggaac tcgtgttgaa gaaacatcaa taacagctcg tcaattagaa gctcgttttg
      421 ctacattagt accggaaatg agaatgataa ctacaactgc aggttcaaat gatgacgcag
      481 gtatatctgc tttgtttaca tcaactacaa ataataagat tcaaatgaca gttgtttgtt
      541 ctaaaaaata tgaacgtaaa cgtacaatat ttgaaatagc ggaggtactt cgtaaagaac
      601 ttgatcgtta tcctgaaatt gttgactatc aatgcgaggt ttcaagtggt atgggtggtg
      661 gaagagcatc taccgtaaat attgaaattt atggttacga ttttgatact accactaatt
      721 ttgcagaggc agtaaaacaa agaattcgca ataatgttac aggtgcacgt gatattgata
      781 ttagtcgtga tgaagatcgt gccgaattaa aaatcgttgt agataaagaa aaaattgcca
      841 gtcatgggtt aagctcatca gcaatatcta tgtatgttag aaaccgtgta aacggatata
      901 ctgcaggata tcttaaagaa gacggagatg aatacgatat tcgtgttcgt ttgaaagaag
      961 agaatagaaa ttctatatct gatatagaaa atatgacgat acccacagct aaaggtaatt
     1021 taataaaact taacgagatt gcatcagttc aagagtattg gtctcctcct gagatagagc
     1081 gtaagagtcg tcaacgtatt gttactgttg ccgtaacccc atacgaaacg tctttgggag
     1141 agttggcaac tgcaattcaa aaagaagttg acaaaatgga tatgccggca ggcataacaa
     1201 tacgttatgg tggtgattat gaggatcaac aagaaacatt tggcgattta ggaatgttgt
     1261 tagctttgat tattatgctt gtatacattg ttatggcatc acaatttgaa tcatttgcaa
     1321 aaccatttat tattatgatg tcagtaccgt ttgctataac aggtgttata tttgctctgt
     1381 taattacaaa tacatctctt gatatgattg gagcattagg tgttgtaatg ttagttggta
     1441 ttgtagttaa gaatggtatt gtgttagttg attataccaa tttgatgcgc gacagggggt
     1501 acgaattgaa tgaagcaatc gaattgtccg gtgcatctcg tctgcgtcct gttttaatga
     1561 ctgctttgac taccgtatta ggtatggtac caatggcttt gagtagcggc gaaggttctg
     1621 aaatgtggca gcctatgggc atagtcgtta taggtggttt gttagtttca acaataataa
     1681 ccctaatagt tgttcctgta ttgtatgcta tcatgtcaaa acatggtgaa cgcaataaag
     1741 aagagaaggc tcgcaaagag tttatattca tgcaattatc agataaataa aaaaatatga
     1801 aatcagtatt tatagttttc aatcaagcaa acacagaacg tgtagagtat atgctggatc
     1861 aactaaaagt aagcggattt actttttttg agcaggtaca aggtagggga actaatggag
     1921 gagaaccacg aagaggtact cacacatggc ctgagatgaa ttctgccgtt atggcggttg
     1981 tagaagatga tcaagttccg gaattactta gtactgtaaa aaaacttgac aatagaaaca
     2041 aggaagtcgg cgtgcgcgca tttgtatgga atatagaaca aacagtttaa ttccatttga
     2101 ttaaatgatg attgaatacc gaatatctag agatggatat tcggtatttt ttttgtcaaa
     2161 tgttaaaatt tccagcataa tttgccaaaa gttatcaaca aatatgctgt taaacacaat
     2221 attttttcca gtgaaacgtt ttttaactaa ctttgcgcat tcgtttttta atgtgcaagt
     2281 catataaatt tgaattatgt atattataat agaatagaaa ttactaaaaa ctaaataatg
     2341 agaaaaacta aattttctgc tgcttcggca cttttattat tattattttt gggattggca
     2401 ggtgttacat ctgctcagca attacctaat agtaactttg aagcttgggg gaattctttc
     2461 gacggtaatc ctcaaccatc aaattggaca ggttctaatg ttacgcaggt tggtatgaaa
     2521 tttactttag tataccaatc aacagatgct catacaggat catatagtgt taaaatggct
     2581 gacaaagaag tcggtgctat gggaattacc gaaatttcac cttcatggtt gactttagga
     2641 actccttgga gctatttgga aggcttgagt acaagttctg caaccgcagg taccgatggt
     2701 ggtataagtt ttgcatatcg tcctgataca atggctgtct ggattaaacg ccagtccaat
     2761 ggtttagaag acatgaatct tgtttattat tcatggagag gccaatcaaa aggagataaa
     2821 tatttgaata aaggaaatgg ttgtacatca actactcact acgatgaaga atctgatata
     2881 agacaatcta ccgataaaaa tagttgtgga acaagtgttc aggcaaccca agtagcagaa
     2941 ggtttgttac gtacaaaaac tgtatataat aattggacat tagttaaggt acctattaca
     3001 tattataatg atcagatacc tgaaaagatg aacataatct tgtctgcaag taattatcct
     3061 aattttagag caaacagtgg tttgtatgat ggtaattcat tgttggtaga tgacattagt
     3121 cttatttata gttctaaaat tcacgaaatt cgtttaaacg gtattgcgtt gccgggattt
     3181 aaaactaata ctaccgaata tacatacgaa attccaaaag ggtcttcgat acctacaata
     3241 acatgttatc gtagtggtcg tttgttgagt ggaagtgaaa tatccgttaa ttacgctgca
     3301 gctggaactc caactcaaat cacagtaaat gccggagacg gaagttctac aacaacatat
     3361 aaagtaacat ttgtatatac gcgttcgcaa aactgcaaat taaacggaat atcggttaat
     3421 ggtactccta tatcaggatt caatagttct gtaacaaatt acacagtagc actaccattc
     3481 ggaactacag aatttccgac tattacttat acaaaagcag atgatgctca aacagttaca
     3541 attgagcctt gtgcaatccc cggtacggca agagttgttg taaccgctga aaatcctgat
     3601 tatagcagca catacttaat taactttact gtaggtgaac taagtgatac tactttggaa
     3661 gatatattgg taaatggagt ttcaataaca ggttatgctc ctacaaagac tatatatagt
     3721 gtaaatgtgc cggttggaac aacagaagct cctgttttgt cttacaagtc tgcttatgct
     3781 gatggcttac aaacaattgt agttagtcaa aaaggattaa gcgaaacttc aactattacg
     3841 gtatctgctc ccggagcttc aacatcacgt atttataaga ttagttataa tattacagag
     3901 tcatcttatt catacttaca agatattaaa ttagatggag ttattctaac agatttcaat
     3961 ccatcaacac ttacatatac ttgaatgtgg ttggcttgga gcggcgtagt gtcggtgcgg
     4021 atcgtcgctt tattaaacag gctggtgttc atcaccatct gctcactcac cttgatgaga
     4081 ttgggcaaat cactcactgc aacactatca ccctgcttaa attgtagctc ccgcaagatg
     4141 atattgtcgt tcgttttccg atttccctga attt
//
LOCUS       DAOPID010000133         4170 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_28970_length_4170_cov_3.459781, whole genome shotgun sequence.
ACCESSION   DAOPID010000133 DAOPID010000000
VERSION     DAOPID010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28970_length_4170_cov_3.459781"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="PK641_05120"
     CDS             complement(<1..848)
                     /locus_tag="PK641_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55762.1"
                     /translation="MNPFQEAYSVCVRNDAGLSVLRIYWWDGGSACNWSTRPDMTYNG
                     DGWWCYDTGNAKNFKVSGSDDSFQSSEIYNAGGFADKYYILSSDKQLTETTPPIPTGL
                     YISADIPNKVAPSTTVTLTANNATSLCEWSYSDNGTHWTSYLGAVSGPNSTIITPNPS
                     SNTYYRVTSGSESATYQILMETMAFAITGGVNGTPAWHTTPTTADMFQADESSRMVMS
                     FIYSSGQKFKILAKDGGVADVDWGWGSQLNVTNGANISGGTGDLQIDNFANGDAVTLV
                     LTKSGGT"
     gene            complement(1004..2605)
                     /locus_tag="PK641_05125"
     CDS             complement(1004..2605)
                     /locus_tag="PK641_05125"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HPG55763.1"
                     /translation="MNIDEKIMIVYKKTILVFINLYIVLSIQSQVLPDFVKAVPCYGD
                     VQTHRVDIKIHRVLGGFGPYSGYAPVMVGDLDGDKYPELVTIREDYRNNGSCVLVVKG
                     GEWGKHALICFGSDFGSDGVSLGYASGAVALARVKDGAGRMIGIIYIVNGTADAKMIQ
                     AYRYNSIEDISLWKESPLMNNYYGNPGIADLNADGKPEVYVGNEVFDAYSLQPLGVGE
                     GNIGQHLQHDGAMFMMSVAVDVIPSNKGLELICGNQIYSLDASDLNLYQTIGIKHDGS
                     SLAADFDGDGNIEILVRSTLGKLSMYSPNNNKAIFVDAYSMASYPAVGDIDGDGSPEI
                     IGLKSNTRMVAYKYNAKHKRLYEFWSMSHTDVSSQTLMTLFDFDGDGKQEIVYRDETL
                     LRIINGSGKNHITGKDTIRAGKRVAYTLCSMNVSSLTKSEYPVIADVDADGEAEIIVP
                     GTMFGTYTKESTTINIFKSHQDKWMPTRPVWNQYAYDALNINSDLTVPYNLVDKTYTF
                     PDDTNHFNNFLQQMVIMGSNGGFCR"
     gene            2709..3995
                     /locus_tag="PK641_05130"
     CDS             2709..3995
                     /locus_tag="PK641_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HPG55764.1"
                     /translation="MKGNLSTICVQGGWQPKSGEPRVLPIYQSTTFKYDTTEQMADLF
                     DLKTNGYFYTRLQNPTNDIVASKIADLEGGVAAMLTSSGQAANFYAIFNICEAGDHFV
                     SATSIYGGTYNLFAVTMRKLGIDVTFVDADSSEEEIDKAFRPNTKALFGETIANPVLS
                     VLDIEKFARIAHKNGVPLIIDNTFPTPINCRPFNFGADIITHSTTKYMDGHALTVGGC
                     IVDSGNFDWETQHDKFKGLTEPDPSYHGLAYCKSFGKGAYITKATVQLMRDLGSIQSP
                     NNAFLINLGLETLHLRMPRHCENAQKIAEYLEAQDQVAWVNYSGLKSSKYYELAQKQF
                     VNGASCGVVTFGLKGGKEKSIKFMDSLKLACIVTHVADARTSVLHPASHTHRQLSDEQ
                     LIEAGIQPDLIRFSVGIEDANDIIADIKQALACAYK"
     gene            4002..>4170
                     /locus_tag="PK641_05135"
     CDS             4002..>4170
                     /locus_tag="PK641_05135"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HPG55765.1"
                     /translation="MPVNIPKGLPAIETLKKENIFVMDNERASTQEIRPLRIAVLNLM
                     PIKITTETDLIR"
BASE COUNT         1314 a          828 c          722 g         1306 t
ORIGIN      
        1 gttccccctg attttgtaag aaccaaagtt accgcatcac catttgcaaa attatctatt
       61 tgcaaatcgc cggttccacc acttatgttt gcgccatttg taacatttaa ttgtgaaccc
      121 catccccaat ctacgtctgc tacaccaccg tcttttgcta aaattttaaa tttttgaccg
      181 ctgctataaa taaagctcat taccattcta cttgattcat cagcctgaaa catatctgca
      241 gtagttggtg ttgtatgcca tgccggagtc ccgtttacac caccggttat ggcaaatgcc
      301 atggtttcca ttaatatttg atatgttgct gattctgagc ctgacgttac acgataataa
      361 gtgttgctac ttggatttgg agttattatg gtagaatttg gaccacttac ggcaccaaga
      421 tatgaggtcc aatgggttcc gttatcagaa taactccatt cgcataagga tgttgcatta
      481 tttgcagtaa gtgttactgt tgttgaagga gccaccttgt taggtatatc tgctgatata
      541 tataatcctg ttgggatagg aggtgttgtt tcggtaagtt gtttgtctga cgataaaata
      601 tagtatttat ctgcgaagcc gccggcatta tatatttctg atgattgaaa actgtcatca
      661 ctgcctgata ctttgaagtt tttggcattg cctgtgtcgt agcaccacca gccatcgccg
      721 ttatatgtca tatcgggtct agtgctccaa ttgcatgcag acccgccatc ccaccaatat
      781 attcgtaaga ccgataatcc cgcatcatta cgtacacata cagaatatgc ctcttgaaaa
      841 gggttcaaaa gaagacaaag caaaaggagc aataccctgg ttatcgattt attcatatag
      901 cttttgaagg tcagggcaaa gttataaaaa agttatgtaa attaaaatct tataaaccac
      961 aaaataggca ttaattcaat caaacgattg caaatgatga tttttatcta caaaagcctc
     1021 catttgaacc catgattacc atctgctgta gaaaattatt gaagtgattt gtatcatcag
     1081 gaaaagtata tgttttatca actagattat aaggtacagt aagatccgaa ttaatattta
     1141 atgcatcata ggcatattgg ttccatacgg ggcgagtcgg catccatttg tcttgatgcg
     1201 atttaaaaat attgattgtg gtactttctt ttgtgtatgt gccaaacata gtacccggga
     1261 ctattatttc tgcttcacca tctgcatcaa catcagctat tacaggatac tcgctttttg
     1321 ttaatgatga aacattcata gaacacagcg tgtatgcaac tcgtttccct gctctaatag
     1381 tatcttttcc tgttatatga tttttaccac tgccgttaat tattctaagt aatgtttcgt
     1441 ccctatatac tatttcttgt tttccgtcgc catcaaaatc gaataaggtc attagtgttt
     1501 gcgatgaaac atcggtgtgt gacatactcc aaaattcata taaacgttta tgctttgcat
     1561 tatatttgta tgcaaccatt cttgtgttac tttttaatcc tataatttca ggagatccgt
     1621 cgccatctat atctccaaca gccggatatg atgccatact atacgcatca acgaatatcg
     1681 ctttattatt atttggcgag tacattgaaa gcttacccaa agtactgcgt actaatatct
     1741 cgatatttcc gtcaccgtca aaatcggctg ctaaagaaga tccgtcatgt tttattccga
     1801 tggtctgata caaattcaaa tcggatgcat ctaatgaata tatttgattt ccacatatta
     1861 attcaagccc tttattactc ggaataacat caacagctac agacatcata aacattgctc
     1921 catcgtgctg taaatgctgc ccaatattac cttctcctac tcctaaaggc tgtaaactat
     1981 atgcatcaaa aacttcgttg ccgacgtaaa cttcaggctt tccatctgca tttaaatctg
     2041 ctattcccgg attgccgtaa taattattca ttaaagggga ttctttccaa agggatatat
     2101 cttcaattga attataacga tatgcttgta tcatttttgc atcagccgta ccattaacta
     2161 tatatattat gccaatcata cgtcctgctc cgtcttttac acgagccaat gccactgctc
     2221 ctgatgcgta gcctaaagag actccgtcag aaccgaagtc cgaaccaaaa caaataagtg
     2281 catgttttcc ccattctcct ccttttacaa ctaaaacaca actgccatta tttctgtagt
     2341 cttcacgtat ggtaactaat tcaggatatt tatctccatc taaatctccg accatcacag
     2401 gtgcgtaacc ggaatatggt ccaaagccac ctaaaacacg atgtatttta atatctacac
     2461 gatgagtttg cacgtcacca taacaaggca ctgcttttac aaaatcaggt aagacttgcg
     2521 attgaatcga taaaacgata tatagattta taaaaacgag aattgttttt ttatacacaa
     2581 tcattatttt ttcgtcaata ttcacaaagg taaagatttt tgtagaatat tactattgcc
     2641 attcaaataa ttatactttt cttttgatag tttttttgta actttgcgcc aattacaaaa
     2701 gaaatatcat gaaaggaaat ttaagcacta tatgtgttca aggtggatgg cagcctaaat
     2761 cgggcgaacc acgcgtatta cccatttatc agagtactac attcaaatat gatacaacag
     2821 aacaaatggc agatttgttc gacttaaaaa cgaatggata cttttacact cgcttgcaaa
     2881 atccgactaa tgatatagtt gcatctaaaa ttgcagacct tgaaggtgga gtggccgcaa
     2941 tgctaacatc gtcaggtcaa gctgctaatt tttatgcgat atttaatata tgcgaagccg
     3001 gtgatcactt tgtaagtgca acatcaatat atggaggcac atacaacttg tttgcagtta
     3061 ctatgagaaa attaggtatt gatgtaactt ttgtagatgc agacagttcg gaagaggaaa
     3121 ttgacaaagc atttcgccca aacacaaaag ctctttttgg agaaacaata gcaaatcctg
     3181 tattgagtgt acttgatatt gaaaaatttg cacgcatcgc acataaaaat ggagtcccat
     3241 taataattga caacacattc ccaactccaa tcaactgccg cccatttaat tttggtgccg
     3301 acatcattac acattcaaca accaaataca tggatggaca tgctctaact gtaggtggat
     3361 gtattgttga tagtggaaac tttgattggg aaactcaaca cgacaaattt aaaggtctta
     3421 cagagccgga tccttcgtat catggattag catattgcaa atcttttggt aaaggcgcat
     3481 atataacaaa agcaactgtt caattgatgc gcgacttggg ttcgatacaa tctcctaata
     3541 atgcattttt gattaacctt ggcttggaaa cattacattt acgtatgcct cgccactgcg
     3601 aaaatgcaca aaaaattgca gaatacttag aagctcaaga ccaagttgca tgggttaatt
     3661 atagtgggtt aaaaagcagc aaatactacg aattagcaca gaaacaattt gtaaatggtg
     3721 catcttgcgg tgttgttact ttcggtctta aaggcggaaa agaaaagtct atcaaattta
     3781 tggatagcct taaattagct tgcattgtta cacacgtagc agatgcccgt acttctgtat
     3841 tgcatccggc aagccataca cacaggcaat tatctgacga gcaattaatt gaagcaggca
     3901 tacaacctga cttaattcgt ttctcagtag gaattgaaga tgccaacgac attattgcag
     3961 acataaaaca agcattagct tgtgcatata aataaattga tatgccagta aatattccaa
     4021 agggattacc agcaattgag acattaaaaa aagagaatat ctttgtaatg gacaatgagc
     4081 gagcctcaac acaagagatt cgtccactaa gaattgctgt tctcaacttg atgcccataa
     4141 aaataactac cgaaacagat ttgataagat
//
LOCUS       DAOPID010000134         4148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_29176_length_4148_cov_3.933056, whole genome shotgun sequence.
ACCESSION   DAOPID010000134 DAOPID010000000
VERSION     DAOPID010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29176_length_4148_cov_3.933056"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="PK641_05140"
     CDS             <1..853
                     /locus_tag="PK641_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPG55766.1"
                     /translation="KKSPKVYLRHLPFVLSVLSIISIIFALARPQKSDSWSNQTTEGI
                     DIVMALDISGSMLAEDLKPNRLEAAKLVASKFISDRSNDNIALVVFAGESFTQCPLTV
                     NHAELINLMKGVKSGMIEDGTAIGSGLATGINRIKDSQAKSKVIILLTDGSNNRGEIA
                     PTTAGEIAKTFGVRVYTIGVGTRGMAPYPFQTPYGVQYQDVEVDIDEPTLQQIASTTG
                     GAYFRATDNDSLESIYSEIDKMEKTKINVKEFSKKEELYHIFSLLAVVFLVSAVALRN
                     TVLKSIP"
     gene            914..1930
                     /locus_tag="PK641_05145"
     CDS             914..1930
                     /locus_tag="PK641_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPG55767.1"
                     /translation="MFRFARPEYLYLLLLIPVLIGFFYYSLIIKNKRLRKLGEKSLLK
                     NLMLTASHWRPRVKFYAVLVAIILCIFALAGPQFGTKLEKVKRQGIEMMVAVDVSNSM
                     LAQDVSPDRMSRAKQVLSRLIDNLQDDKVGLLVFAGDAYVQMPMTTDVGSAKMFLSNI
                     NPGMVPVQGTAIGSAIRMSMGLFSTQENISRSIIIITDGENHEDDAVSTAKMAFENGV
                     TVNVLGLGTPQGSPIPINGTNSFRKDSDGNVVVTKLNEQMCQEIAQAGGGVYVRADIA
                     GTAVRLLSKELNKLDKADIEAEVYTNYNEQYAPLLWLALIFLLVEACLLERKNPLFKN
                     VKLF"
     gene            1935..2615
                     /locus_tag="PK641_05150"
     CDS             1935..2615
                     /locus_tag="PK641_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG55768.1"
                     /translation="MRKLLFCTILSIGIPFVVLAQEANSDIRSGNKLYKDQKYTDSEI
                     EYRKGIQKDKKSIESFYNLGNSLYQQKKYKEAAEEFEKAATLTDDKDKLASVYHNMGN
                     SYYQAEDYGKSIDAYKKSLKNRPSDNDTRFNLALAQQKLQQQQQQQQQQDQNQDKKDQ
                     KDQQQQQDQKDKKDQQKPQQQQQKQQELSKEAAQQILDSYSQDEKEVQEKVKEQQQAG
                     QRHLEKDW"
     gene            2617..>4148
                     /locus_tag="PK641_05155"
     CDS             2617..>4148
                     /locus_tag="PK641_05155"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HPG55769.1"
                     /translation="MKTKILVILGLCMLAVSVYAEDLQFSASAPEAVVEGDQFRVTYT
                     LNKEGKDLRVPDMSDFDVLMGPSTSRSSSMQITNGSMTKTMSISYTYILAGKAPGKYV
                     LAPATILVDGQKYSSNQLEIKVLPPDQKVPSSSSTQSSNQVPVAQSNTSANISDKQIF
                     VRQTLSKTKVYEQEAILVTYKLYTRNDVSNIPSAKFPEFKGFYVQEIDLNKNQQFVTE
                     HYDGLNYNTYVLKQCLLYPQQSGTLNIESGKLDIVLRVRVNNRAQSIFDSFFDSYQEV
                     RKTLNIPSAKVEVMPLPSGAPASFSGAVGTYKLSSTISSIKLKANDAVTIKVAISGNG
                     NLKLLKNPEITFPTDFDIYDPKVETNIKAAPTGTSGNRNIEYLAIPRFGGDFEIPSAE
                     FTYFDLNTKKYKTLLTPAYSIEVEGTSDETAVVSSNYVSNKESVKNIGSDIRYISSDL
                     TLKPKNDFFLGSASFWMAIILPFIIFLVLVIVLQKQARENANVALRKTKRANKQARKR
                     LKA"
BASE COUNT         1478 a          633 c          797 g         1240 t
ORIGIN      
        1 taaaaaatct cctaaagttt atttgagaca tctgccattt gtattgtctg ttttatctat
       61 tatatcaata atttttgcat tagcacgtcc tcaaaaaagc gatagttgga gtaatcaaac
      121 tacagaaggt attgacatag ttatggctct tgatatatcc ggaagtatgt tagccgaaga
      181 tctgaagcct aatcgcttgg aagctgcaaa attggttgca agtaaattta tctctgatcg
      241 ttcaaacgat aatattgcac ttgtagtatt tgccggtgaa agttttacac aatgtccttt
      301 gacagtgaat catgctgaac tcatcaattt aatgaaaggt gtaaagagtg gaatgattga
      361 agacggaaca gcaataggtt ccggtttggc aactgggatt aatcgtatta aggatagtca
      421 agctaaatct aaagttataa tcttattgac tgatggttct aataatagag gagagattgc
      481 accaacgaca gccggtgaga ttgcaaaaac atttggagtg cgtgtttata ctattggtgt
      541 tggtacgaga gggatggctc cttatccatt ccagacacct tatggcgtac aatatcaaga
      601 tgtagaagta gatatagacg aaccaacttt gcagcaaatt gcttcaacta ccggaggtgc
      661 ttattttaga gctactgata atgatagttt ggagtcaatt tattctgaga ttgataaaat
      721 ggaaaaaact aagattaatg taaaagaatt cagcaaaaag gaagaattgt accatatttt
      781 ttcattgtta gcagtcgtat ttttagtttc tgctgttgct ttacgtaaca ctgttttaaa
      841 atcaattcct tgatgtatcg aattgatatt gcgtattaaa tgataaagaa ttaatttctt
      901 aaagatttta aagatgtttc gatttgctcg tcccgaatat ttatatttat tattactgat
      961 accggttttg atcggtttct tctactatag cttaatcatc aagaataaac gtttaagaaa
     1021 attaggcgaa aaatcattac tcaaaaattt gatgctaact gcttctcatt ggcgcccaag
     1081 agtaaagttt tatgcggttt tagtcgcaat tatcttatgt atttttgctt tagcaggtcc
     1141 tcaatttggt acaaaattag aaaaagttaa gcgtcagggt attgaaatga tggtagcagt
     1201 ggatgtatca aattcaatgt tagctcaaga tgtgtcacct gacagaatgt cgcgtgcaaa
     1261 acaagtacta tccagattaa tagacaactt acaagatgat aaggtaggtt tattggtatt
     1321 tgccggagat gcatatgttc agatgccaat gactacagat gttggatcag ctaagatgtt
     1381 cttatctaat ataaatcccg gaatggtacc tgtacagggt actgcgatag gttctgctat
     1441 acgaatgtct atgggtttat ttagtacgca agaaaatatc agtagatcaa taataatcat
     1501 tactgatggt gagaaccacg aggatgatgc agtttctact gcaaaaatgg catttgaaaa
     1561 tggtgttacc gtaaatgttc tgggtttggg tactcctcaa ggatcgccaa tcccaataaa
     1621 cggaacaaat agttttagaa aagacagtga tggtaatgtg gttgtaacca agttgaatga
     1681 gcaaatgtgt caagaaatag ctcaagcagg tggaggagtg tatgtaagag cagatatcgc
     1741 aggaacagcg gtacgtttgt tatctaaaga attgaataaa ttggataagg cagatataga
     1801 agctgaagtc tatactaatt ataatgagca atatgctcct cttttgtggt tagcattgat
     1861 atttttgctc gttgaagctt gtttattgga acgtaagaat cctttattta agaatgttaa
     1921 attgttttag tatcatgcga aaattattgt tttgtactat cttatctatt ggcatacctt
     1981 ttgtagtatt ggctcaagag gcaaattctg atatccgtag tggaaataaa ttatacaaag
     2041 accaaaaata taccgattca gaaattgagt acagaaaagg tattcaaaaa gacaagaagt
     2101 caatagaaag tttttacaat cttgggaata gtttgtatca acaaaaaaaa tacaaagaag
     2161 ctgccgaaga atttgaaaaa gcggctactt taactgatga taaagataaa ttggcatcag
     2221 tatatcataa tatgggtaat tcttattatc aagcagaaga ttatggaaag agtattgacg
     2281 cgtataaaaa atcgcttaag aatcgtccat cagataatga tactcgtttt aatttagctt
     2341 tagcccagca gaagttacag caacaacagc aacagcaaca acagcaagat cagaatcaag
     2401 ataaaaaaga tcagaaggat caacagcaac agcaagatca aaaagataag aaagatcaac
     2461 aaaagccgca gcaacagcaa caaaaacaac aagaattgtc taaggaagcg gctcaacaaa
     2521 tattagactc atactctcaa gacgagaaag aagtacagga aaaagtaaaa gaacaacaac
     2581 aggcaggaca aaggcatttg gaaaaagact ggtaatatga aaacaaaaat attagttata
     2641 ttaggcttat gtatgttagc cgtttcagtg tatgctgagg atcttcaatt ttcagcgtcg
     2701 gcacctgaag cagtagttga aggcgaccag tttagagtta catacacact aaataaagaa
     2761 ggcaaggatc ttcgagttcc tgatatgtct gattttgacg tgttaatggg gccttctaca
     2821 tcacgtagtt caagtatgca gattactaat ggttcaatga ccaaaacaat gtcaatatcg
     2881 tatacttaca tattagcagg aaaagctccg ggtaaatatg tattagctcc tgctacaata
     2941 ttggttgatg ggcaaaaata tagttctaat caactagaaa taaaagtact tcctcctgat
     3001 caaaaagtgc cttcttcatc aagtacgcaa agttcgaatc aagttcctgt agctcagagc
     3061 aatacatctg caaatatttc tgataaacaa atattcgttc gtcagacttt atcaaaaaca
     3121 aaagtctacg aacaagaggc tattttagtc acatataaat tgtatactag aaatgatgtt
     3181 agcaatattc caagcgcaaa atttcctgaa tttaaaggtt tttatgtgca agaaattgat
     3241 ctaaacaaaa accaacagtt tgtcacagaa cattatgatg gtttgaacta caatacatac
     3301 gtcttaaaac aatgtttgct atatccgcaa caatccggaa ctttaaatat tgaatcagga
     3361 aaattagata tagttttaag agtacgtgtt aacaataggg cacaaagtat atttgatagc
     3421 ttctttgatt cgtatcaaga agtaagaaaa actttgaaca taccatcggc taaagttgag
     3481 gtaatgcctt tgccatcagg tgcaccggca agttttagtg gggcagtagg gacttacaaa
     3541 ctgtcttcaa ctatttcctc aatcaaatta aaagccaacg atgcagtaac aattaaagtg
     3601 gctatatcag gaaatggaaa tttaaaattg cttaagaacc ctgaaataac attcccaaca
     3661 gattttgata tttatgaccc taaggtagaa acaaatatca aagcagcacc aacgggaact
     3721 tccggcaata gaaatattga atatctggca attccacgtt ttggaggaga ttttgaaata
     3781 ccctctgccg aatttacata ttttgattta aatacaaaga aatataaaac attgcttact
     3841 cctgcttatt caattgaagt agaaggtaca tctgacgaaa cagcagttgt ttcttctaat
     3901 tatgtgtcga ataaggaaag tgtaaaaaat atagggtcag atataagata tattagcagc
     3961 gatcttactt taaaaccaaa gaatgatttc tttttgggat cagcatcttt ttggatggca
     4021 attattttac cgtttattat tttcttggtt ttggtaatcg tgttacaaaa acaagcaaga
     4081 gaaaatgcca atgtcgcatt acgtaaaaca aaacgagcta ataaacaagc acgtaaacgt
     4141 ctgaaggc
//
LOCUS       DAOPID010000135         4148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_29179_length_4148_cov_3.220376, whole genome shotgun sequence.
ACCESSION   DAOPID010000135 DAOPID010000000
VERSION     DAOPID010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29179_length_4148_cov_3.220376"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1626)
                     /locus_tag="PK641_05160"
     CDS             complement(<1..1626)
                     /locus_tag="PK641_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPG55770.1"
                     /translation="MKKLHQRMRFRRCLCVFFLTLASISSLFASSYGTIFSETRTDFR
                     DESIYFVMTTRFYDGDVSNNTQCWDGQSYNVGDPAWRGDFKGLIEKLDYIKALGFTAV
                     WITPIVENASGYDYHGYHASNFSKVDHRYESEDVAFEDLVTAVHSKGMKIILDIVINH
                     TGNFGESNLCPLFKRDWDADQSNLDDCMIPLEASEGGLLPSNYYDLESGKQYAARLSK
                     MKNTPDENGQKNNYDSHNYWHHYAQFNWDETNRWWGQIAGDCVDLNTENPAVYNYLIQ
                     CYTTFIAMGVDGFRIDTGGHIPRLTFNKAFIPAFQAAAEQYKDKRGGFPFYMYAEVCA
                     RYSSIWYREHPNLSPLYYTWKESQEYDWDVNPESWNSIVALEGDKCDTHTNHKSVQAT
                     AIADLDNKGAQPISNNAFLNGNDYHAPDYSKASGLNVIDFTVHHSFVDIGTAWNVANR
                     DNDQYYNDATWNVVYVDSHDYAPNGAPEDKRYSKGTTAWAENLCLMYTFRGIPCVYYG
                     SEIEFKAGATIDKGPNIPLKESGRAYYGGYIKGS"
     gene            1920..>4148
                     /locus_tag="PK641_05165"
     CDS             1920..>4148
                     /locus_tag="PK641_05165"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HPG55771.1"
                     /translation="MSKNVPKVGFFQQIKSFFTDEKVRFIIGLIILFGNLYVCISLIS
                     FLFTGSEDFNLLGTEVQIPDFDDKPYMNVCGAGGAIVANFFINKWFGISSFFLIFWGF
                     SAGYKLVNPTKQYSLFKQLIVCVFFTIWISVALGFVLPDIEAIPYIKLGGNHGNAIAA
                     FLSGVMGKTGLVLLLVGVILIFCIIAFVSALPAIRKWMDFSSWKIFKKKDKSITESLD
                     NTDTTINEENLSDSKKDDDIDFTITDTMDDILESEDESDEKEELIIQPINEDDEIGFE
                     VNQSTDEGSEIVDKPIDLLEKYGEYDPKLDLPHYKIPTFNLLKDYNLEDSIDLNEQNA
                     NKQRIIETLKTYGITIKEIKATVGPTITLYEIIPGEGVRIAKIRNLGDDIALSLAAEG
                     IRIIAPIPGKGTIGIEVPNENKQIVPMRSVIASKKFQESTFDLPLVFGKTITNEVFMV
                     DMCKMPHLLVAGATGQGKSVGLNAIITSLLYKKHPSQLKFVLVDPKKVEFSIYSTIEK
                     HFLAKLPDNEEAIITNVNKVVQTLKSLCKEMDTRYDLLKKAHVRNIKEYNDKFVKRQL
                     NPEKGHCFLPYIVVVIDEFGDLIMTAGKDVELPIARIAQLARAIGIHMIIATQRPTTN
                     IITGTIKANFPARMAFRTAASIDSRTILDAPGADKLIGRGDMLFSQGSDLVRIQCAFV
                     DTPEIEAICSYIGDQKGYPAAFALPEVESENGGDTDIRGIDMSQRDAMFEEAARLIVI
                     HQS"
BASE COUNT         1390 a          681 c          720 g         1357 t
ORIGIN      
        1 tgagccttta atataacctc cataatatgc tcgtcctgat tcttttaatg gaatattagg
       61 acctttatct attgttgcgc ctgctttaaa ctctatttca gaaccataat atacacatgg
      121 aataccacga aaagtgtaca tcaaacataa attttcggcc catgctgtgg ttccttttga
      181 ataacgctta tcttctggtg caccatttgg agcataatca tgtgaatcta catatactac
      241 attccatgta gcatcattat agtattgatc attatctcta tttgcaacat tccaagcagt
      301 gccgatatca acaaagctgt gatgcacagt aaaatctatc acatttaatc cggaagcttt
      361 actataatca ggtgcgtgat aatcatttcc atttaaaaat gcattatttg aaattggttg
      421 tgctccttta ttgtctaaat cagcaatcgc agtagcttgt accgatttat gattagtatg
      481 tgtatcacat ttatctcctt ctaatgctac aatactattc caagactcag gattaacgtc
      541 ccaatcatat tcctgagatt ctttccatgt ataatataac ggggataaat taggatgctc
      601 acgataccat attgaagaat atcttgcgca aacttcagca tacatataaa acgggaatcc
      661 gccacgtttg tctttatatt gttcagcagc agcctgaaat gcgggtataa atgctttatt
      721 aaatgtcaaa cgaggaatat gacctcctgt atcaatacga aatccgtcga cacccattgc
      781 aataaatgtt gtataacatt gtataagata attataaaca gccggatttt cagtatttaa
      841 atcaacgcaa tctcctgcaa tttgtcccca ccaacgattt gtttcatccc agttaaattg
      901 agcatagtga tgccaataat tgtgagaatc ataattgttc ttttgaccat tttcatcagg
      961 tgtatttttc attttcgaca aacgagcagc atattgcttg cctgattcca aatcatagta
     1021 gtttgatggt aataatccac cttctgaagc ttctaatgga atcatgcaat catcaagatt
     1081 actttggtcg gcatcccaat cacgtttaaa caatggacat aagttagact caccgaaatt
     1141 accggtatgg ttaattacaa tatctaatat gattttcatg ccttttgagt gtacggcagt
     1201 aactaaatct tcgaatgcta catcttctga ttcataacga tggtcaactt tcgaaaaatt
     1261 acttgcatga taaccatgat aatcgtatcc ggaagcattt tcaactatag gagttatcca
     1321 aactgcggta aatcccaaag ctttaatgta atccaacttt tcgattaaac ctttaaagtc
     1381 tccacgccat gcagggtctc caacattgta gctttggcca tcccaacatt gagtattgtt
     1441 tgatacatca ccatcataaa atcgagtcgt cattacaaaa taaatagact catctctaaa
     1501 atcagtacgt gtttctgaga agattgttcc ataagaggaa gcaaacaagg aagaaattga
     1561 tgctagtgtc aaaaagaaaa cacacaagca tctacggaaa cgcatccgtt gatgtaattt
     1621 tttcattttt tcataaattt ttaaggttcg tatctgtata tattgagtat atcagaatat
     1681 cagccgcaaa tgtaatcgtt tttttattta gtacaatttt aaatcgtaac tttaatatat
     1741 tttaatataa aaaaaacttg tataaactat aaaggaaaat tgttgaaaac tcatgagcaa
     1801 aaaatatatt attagtttat tatttagtaa ttttgccata tcaaaatgga aaggaagtct
     1861 gaacagcgat gtaggtattt taattattgt caataaactc ctttcaatca gtattatata
     1921 tgtctaaaaa cgtccccaaa gtaggatttt tccaacagat taaatctttt tttacggatg
     1981 agaaagttcg atttattatc ggattaatca ttctgtttgg caatttatat gtatgcatct
     2041 cacttatatc atttctattt acaggtagcg aagattttaa tttactaggt actgaggtac
     2101 aaattcctga ttttgatgac aagccatata tgaatgtatg tggagccgga ggtgctattg
     2161 ttgcaaattt ctttattaat aagtggtttg gaatatcatc attctttttg atattttggg
     2221 gcttttctgc aggttacaaa ttggttaatc cgaccaagca atactctctt tttaaacaat
     2281 taattgtttg tgtgttcttt acaatatgga tttccgttgc attaggattt gttttacctg
     2341 atattgaagc cataccatat attaagttag gtggaaacca cggaaatgcc attgctgcat
     2401 ttttaagcgg tgtaatgggg aaaaccggtt tagtattact tcttgttggt gttatattaa
     2461 tattttgcat aattgctttt gtttcagcac tacctgctat tagaaaatgg atggattttt
     2521 cttcttggaa aatatttaaa aagaaggata aatctattac ggagtcatta gataatactg
     2581 atactacaat taatgaagaa aatcttagtg attccaaaaa agacgatgat attgatttta
     2641 caataacgga tacaatggac gatattttgg agtcagaaga tgaatctgat gaaaaagaag
     2701 agcttattat tcaaccaata aatgaagacg acgaaatagg atttgaggtt aatcaatcta
     2761 ctgatgaagg ttctgaaatt gtagataaac caatagattt acttgaaaaa tatggcgaat
     2821 atgatcctaa attagatttg ccgcattaca aaataccgac atttaattta cttaaagact
     2881 ataatctcga agatagcata gatttaaatg aacagaatgc taataaacaa agaattattg
     2941 aaacacttaa gacctatggt atcactataa aggaaattaa agcaactgtt ggaccaacta
     3001 ttacattata cgaaataatt cccggagaag gtgttagaat agccaaaata aggaatttag
     3061 gtgatgatat agctcttagt ttagcagctg aaggtatacg tattatagcc cctattccgg
     3121 gcaaagggac cataggtatt gaagtgccta atgagaacaa acagatcgtt cctatgcgtt
     3181 ctgtaatagc atctaaaaaa ttccaagaaa gcacatttga cttaccactt gtatttggaa
     3241 agaccattac taacgaagta tttatggtag atatgtgcaa gatgcctcac ttattagttg
     3301 ctggtgctac cggacaagga aagtccgtag gattgaatgc gattattaca tcattattat
     3361 acaagaagca cccttcgcag ttgaaatttg tattagttga tcctaagaaa gttgagttta
     3421 gtatatactc tacaattgag aaacatttct tagcaaagct gcctgataat gaagaagcca
     3481 ttattaccaa tgtcaataaa gttgtacaaa cattaaagtc tttgtgcaaa gaaatggata
     3541 ctcgttatga tttactcaag aaagcacatg ttcgtaatat taaagagtat aatgacaaat
     3601 ttgtaaagag acaattgaat ccggaaaaag gacattgctt tttaccttat atagtagtgg
     3661 taattgatga gttcggtgat ttgataatga ccgcaggaaa agatgttgag ttaccaattg
     3721 cacgtattgc ccaattagca cgtgctatag gtattcacat gataattgca acacaacgtc
     3781 ctactactaa tatcattaca ggtacaatta aagctaactt ccctgctcgt atggcattta
     3841 gaacagctgc atcaattgac tcaagaacta ttttggatgc accgggtgct gacaaattaa
     3901 ttggtcgtgg agatatgtta ttttcgcaag gtagcgattt agttagaata caatgtgctt
     3961 ttgttgatac tcctgaaatt gaagcaatat gttcgtatat tggagatcag aaaggctatc
     4021 cggcagcatt cgcacttcct gaagtagaaa gcgaaaatgg tggtgataca gatatacgtg
     4081 gcattgatat gagtcagaga gatgcgatgt ttgaagaagc agcacgttta atcgtgattc
     4141 atcaaagc
//
LOCUS       DAOPID010000136         4128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_29361_length_4128_cov_4.463049, whole genome shotgun sequence.
ACCESSION   DAOPID010000136 DAOPID010000000
VERSION     DAOPID010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29361_length_4128_cov_4.463049"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..110
                     /locus_tag="PK641_05170"
     CDS             <1..110
                     /locus_tag="PK641_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPG55772.1"
                     /translation="KNAAELFAKPDVDGGLIGGASLKAPDFKAIIDAFN"
     gene            112..696
                     /gene="rdgB"
                     /locus_tag="PK641_05175"
     CDS             112..696
                     /gene="rdgB"
                     /locus_tag="PK641_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HPG55773.1"
                     /translation="MANSLIFATGNRHKLGEVQSFLGDSFALSCLKDVGITEDIPETA
                     DTLEGNALQKALYVYHKCGCACFSDDTGLEIEALNGEPGVHSARYAGSHKNDQDNVKK
                     VLKLLQGETNRRARFRTVIAYIDKSGESRFFEGEVRGTIIESCHGTEGFGYDPIFMPD
                     GYDCTFAELSLNEKNKISHRARALQKFLLFLKPS"
     gene            693..3020
                     /locus_tag="PK641_05180"
     CDS             693..3020
                     /locus_tag="PK641_05180"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPG55774.1"
                     /translation="MKRFISLLILSIATSTIASAQIAVGSWRTHFSYNSTTQVVQTPN
                     KVFGVADKRLFSISKTDETIDTYTKIDGFSGNGVERIAYNKEKSLLVIVYTTSDIDIL
                     DADGNIYNIPDLYKKSLAVDKTVYQIYFKGNYAYLSCGFGVVVLNTKNMEISDTYIIG
                     DDAAMKPVYGFASDDNYFYALMDDKIKRAKVSGVNLLDYNNWEEGQILLPDESAAFRN
                     LYYYSNCIVVSKTKASAYKYDGQSWQVFYDNSEQKRINISISDDNLFVCNESSFHIYS
                     NSWTKESIENINAEDIASDGSVYWIAGSHRGIIRMEPNKDYKFYKPDGPYISSAQSMT
                     YSKGRMLFAPGYSWMDRGNVEGAVLIFDNNVWTSYTGTSTDAASISPNGIFKDIVSVA
                     VDPDNDNHIFATTYGEGVYEFLDGKAVKLFNNENSVLEDIGGFGVHYVRVDGLTYDAK
                     KNLWLVNSGAEYALKYMTPDGIWHSAPKYASISGQSTLQQIVINSKKQKWVRAARSAS
                     GVFVLDDGSTIDEVSDDKSRFFTSFTDKDGNILKPQYIYSMEEDRSGTMWLGTSNGPI
                     LMNSVNKVFDSNYTCSRIKIPRNDGTNLADYLLDGVNVRAIAVDGADRKWLGTDNSGL
                     YLVSSDGTKTIHHFTTENSPLSSNNINSIKLNEETGEVFISTADGIVSYRSDSTEPVD
                     IAETSKVHVFPNPVRPNYTGLITITGLEENSLVKITDASGNLVYEGTSTGGSISWTGK
                     NYSGSYVSGGIYFVHCFNSSQEENRSVAAKILIVR"
     gene            3239..>4128
                     /locus_tag="PK641_05185"
     CDS             3239..>4128
                     /locus_tag="PK641_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55775.1"
                     /translation="MAASLFLSFNIDPNELNVDRWSAIHNFLFNLLNGVFPYSAQTHL
                     GGYGSPFPVWQILHLPFYALGNVGYSSIFVILLFVLSLCKTRSFLMGFKAILLLCMSP
                     AVWYEITVRSDFMANFLLCATVINYMHYIKFDAERHIILSALIIGMFLSTRWAAAIPF
                     AVYLFPSFYKWNFKKQVSLILLSCLVFALTFLPFILWDYEKLLFFDYSPFVLQTRQGS
                     TWDLLLLIPIAIWTALRFRSNNASYAFQIAFVLFMSVAIAFVHSSLKHGMQYGLFDQF
                     YDICYFNMSLVFVILGLSIK"
BASE COUNT         1285 a          602 c          839 g         1402 t
ORIGIN      
        1 caaaaaacgc agctgaatta ttcgcaaaac cagacgttga tggcggatta atcggtggtg
       61 catctttaaa agctcctgat ttcaaagcaa tcattgacgc tttcaattaa tatggcaaat
      121 tcccttattt ttgcgacggg aaatagacat aaattaggtg aggtgcaaag ctttcttggt
      181 gatagctttg cactttcttg tctaaaggat gtaggaatta cagaagatat tcccgaaacg
      241 gctgatactt tagaagggaa tgcactgcaa aaggcacttt atgtgtatca taagtgtggt
      301 tgtgcatgtt tctcagacga taccggctta gaaatagagg ctcttaatgg cgaaccgggt
      361 gttcattctg ctcgttatgc aggctctcac aagaatgacc aagacaatgt aaaaaaagtt
      421 ttgaaattgt tacaaggtga aacaaatcgc agagctcgtt tcagaactgt tatagcttat
      481 attgataaaa gtggagaaag tagattcttc gaaggagaag tgcgtggaac aattattgaa
      541 tcatgtcatg gaacagaagg atttggatat gatccgatat ttatgcctga tggatacgat
      601 tgtacttttg ctgaattgtc tttgaatgaa aaaaataaaa taagccatag ggcgcgtgca
      661 ttgcaaaaat ttttactatt tttgaaacca tcatgaaacg atttatttct ttgctaatat
      721 tgtcgatagc taccagtacg atagcgagtg cacaaattgc tgtaggatcg tggagaactc
      781 atttctcata caattcaaca acgcaggttg tgcaaactcc gaataaagtt tttggcgttg
      841 cagataaaag attgttttct atatctaaaa ccgacgaaac aattgataca tatactaaga
      901 ttgatggatt tagtggaaat ggagttgaaa gaatcgcata taacaaagaa aaatcattgt
      961 tggttatagt ttataccact tctgatatag atatcttgga tgcagatgga aatatatata
     1021 atattcccga tttgtataaa aagagtttgg ctgtagataa aaccgtatat caaatatact
     1081 ttaaaggaaa ttatgcttat ttgtcatgcg gatttggtgt tgtagtctta aataccaaga
     1141 atatggaaat atctgatacc tacataattg gggacgatgc ggcaatgaag ccggtatatg
     1201 gctttgcatc agatgacaat tatttttatg ccttgatgga tgacaaaatt aaaagggcaa
     1261 aagtaagcgg tgtaaactta ttggattaca ataattggga agaaggacag attttgttac
     1321 ctgatgaatc cgcggctttt agaaatctat attattatag taattgcata gttgtaagta
     1381 aaacaaaagc atctgcatat aaatatgatg gccagtcatg gcaagtattt tatgataact
     1441 ctgagcaaaa aagaattaat atatcaattt cagatgataa tctgtttgta tgtaacgaat
     1501 catcatttca catatattca aattcctgga ccaaagaaag catagagaat attaatgctg
     1561 aagatatagc tagtgacggt agcgtttatt ggattgcagg tagtcatcgc ggaattattc
     1621 gtatggagcc aaataaagat tataagtttt ataaacctga tggaccatat ataagttctg
     1681 cacaaagtat gacatacagt aaaggacgta tgttatttgc tccgggatat tcatggatgg
     1741 atagaggtaa tgtagaagga gcagtcctta ttttcgacaa taatgtttgg acatcgtata
     1801 ccgggacttc aacagatgct gcttctatat ccccgaatgg catttttaaa gatatagttt
     1861 ctgttgccgt cgaccctgat aatgataatc atatttttgc tacaacatat ggtgaaggtg
     1921 tatacgagtt tcttgatgga aaggctgtta aactctttaa taatgagaat agtgtactcg
     1981 aagatattgg aggtttcggt gttcactatg ttcgtgttga cggattgact tatgatgcaa
     2041 aaaagaatct gtggcttgtt aattcaggtg ccgaatatgc tttaaaatat atgactcccg
     2101 atggaatatg gcattctgca cctaagtatg cttcaatatc agggcagagt acattacaac
     2161 agattgttat taactcaaaa aaacaaaaat gggtgagggc tgcccgttct gcgagtggtg
     2221 tttttgtatt agatgacggg agtacgattg atgaggtttc ggacgacaaa tcaagatttt
     2281 ttacctcgtt tacagataaa gacgggaata ttttaaagcc tcagtacatt tattcgatgg
     2341 aagaggatag gagtggaaca atgtggcttg gaacaagtaa tggaccgata ttgatgaata
     2401 gcgttaataa ggtttttgat tcaaattata catgttctcg tataaagatt ccaagaaatg
     2461 acggcacaaa tttggcagat tatctattag atggtgttaa tgtaagagcc attgctgtag
     2521 acggggccga taggaaatgg ttgggaacag ataattcggg cttgtattta gtatcgtctg
     2581 atggtacgaa aacaatacat cattttacta cagaaaacag cccattgtct agtaataata
     2641 ttaattcaat taaacttaat gaagaaacag gagaggtctt tatttctaca gctgatggaa
     2701 tagtgtcata cagatctgat tctacagaac ctgttgatat tgctgagaca tctaaagttc
     2761 acgtgttccc gaatcctgtt agaccaaatt atacgggtct cattacaatt acaggtttag
     2821 aagagaactc tttagtaaaa ataacagatg catcaggaaa ccttgtatat gagggaacct
     2881 cgaccggagg atcaatatca tggacaggta aaaattatag tggttcttat gtttcagggg
     2941 gaatatattt tgttcactgc tttaattcta gtcaggaaga gaatagaagt gttgcggcta
     3001 aaatattaat tgttagatga ataaattgac tcacatttat cttccactgc ttgtatatac
     3061 atgcatcaat gctttattta ttgcaaaata ttcatctaga atttctgttt actatgcttg
     3121 gtctgtgctg gcctatgttg tagtatagtt tgcagttttt gcattctgta tttattattt
     3181 tccaaaaaaa tcagaaaagt tttttaaagt ttgtgttggc gtttctgttg ttctatttgt
     3241 ggcagcatct ttgtttttgt cttttaatat tgatccgaac gagcttaatg tagatagatg
     3301 gtctgctata cacaacttct tatttaatct gttgaatggg gtgttcccgt attctgccca
     3361 aacacattta ggcggctatg gttctccctt tcctgtatgg cagattcttc atttaccatt
     3421 ctatgcctta ggcaatgtag gttattcatc tatttttgta atactactat ttgttttatc
     3481 cttatgtaaa acgagatctt ttttaatggg ttttaaggcg atattgctct tgtgtatgtc
     3541 tcctgcagta tggtatgaga ttacagtgcg gagtgatttt atggctaatt tccttttgtg
     3601 tgcaactgtt attaattaca tgcattacat caagtttgac gcagaacgcc atattatttt
     3661 gtctgcttta ataataggta tgtttttatc aacgcgttgg gctgcagcaa ttccttttgc
     3721 agtatatcta ttcccatctt tttacaagtg gaattttaaa aaacaagtta gtttaatctt
     3781 attatcgtgt ttggtttttg cacttacatt tctacctttc attttgtggg attatgaaaa
     3841 attattgttt tttgattata gtccatttgt attacagacg cgtcagggta gtacatggga
     3901 tttattatta ttaataccaa ttgctatatg gactgctttg aggtttcggt caaataatgc
     3961 ttcttacgca tttcagatag ccttcgtgtt atttatgtcg gtagcaatcg cttttgttca
     4021 ttcttcatta aaacatggaa tgcaatatgg actatttgat caattttatg atatatgcta
     4081 ttttaatatg tcattagtat ttgtaatact aggactatcc ataaaaaa
//
LOCUS       DAOPID010000137         4106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_29539_length_4106_cov_4.487534, whole genome shotgun sequence.
ACCESSION   DAOPID010000137 DAOPID010000000
VERSION     DAOPID010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29539_length_4106_cov_4.487534"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /gene="glmS"
                     /locus_tag="PK641_05190"
     CDS             complement(<1..1025)
                     /gene="glmS"
                     /locus_tag="PK641_05190"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HPG55776.1"
                     /translation="MCGIVGYIGNQQAYPILIKGLHRLEYRGYDSAGVAFMNDKKELN
                     VYKTKGKVSDLESLVESQDVSGTIGIAHTRWATHGEPNNENAHPHYSQSETIALIHNG
                     IIENYSVIKKELLRQGYTFKSTTDTEVLVQFIEYVKKTNKVDLCHAVQIALSQVIGAY
                     AIAVIDKENPNQIVAARKSSPLVVGIGKNEYFLASDATPIVEYTDKVVYVNDEEVAVI
                     DRKNGVKLLNLSDVEQTAEVTTLKMNLSQLEKGGYENFMLKEIHEQQQTLIDCIRGRI
                     NVEGTEVVLSGVVDNKDKFLNAKRIIIVACGTSWHAALIGEFLLEDLCRIPVEVEYAS
                     EFRYRHP"
     gene            1290..2363
                     /gene="carA"
                     /locus_tag="PK641_05195"
     CDS             1290..2363
                     /gene="carA"
                     /locus_tag="PK641_05195"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HPG55777.1"
                     /translation="MRLSLPASLILDNGSVFRGYSFGYKKSTAGEVVFNTAMSGYPES
                     LTDPSYSGQILTFTFPLIGNYGVPSTNISNGISDFLESEKIHTSGIVVSDYSFEYSHW
                     NAKQSLHDWLESNKIPGIYGIDTRELTKILRNEGSMKGKIIIDDYKDIDFIDPNIENQ
                     VAKVSCKEVIEYNNSGKTIVMIDCGVKHNIIRCLLKRGVRLIRVPWDYDYNSMQFDGL
                     FISNGPGDPNTCGKTIANIQKTMQSGKPIFGICMGNQLLCKAGGSETYKLKYGHRSHN
                     QPVREVGTNRCFISSQNHGYAVDNNTLSKDWEVLFENLNDHTNEGIRHKTMPWFSAQF
                     HPEAASGPTDTEFLFDDFLQMIK"
     gene            2377..>4106
                     /gene="carB"
                     /locus_tag="PK641_05200"
     CDS             2377..>4106
                     /gene="carB"
                     /locus_tag="PK641_05200"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HPG55778.1"
                     /translation="MISNIKKVLVLGSGALKIGEAGEFDYSGSQALKALKEEGIFTVL
                     INPNIATVQTSADIADQIYFLPVTPFFVEKIIQKEKPEGILLAFGGQTALNCGVELYK
                     AGILEKYGIQVLGTPVQAIINTEDRELFVKKLNEINVKTINSEAVENAEDARKAAERI
                     GYPVIIRAAYALGGLGSGFCNNESELDVMVEKALSFSPQVLVEKSLKGWKEVEYEVVR
                     DRFDNCVTVCNMENFDPLGIHTGESIVIAPSQTLSNSEYHKLRALAIKIIRHIGIVGE
                     CNVQYALDPESEDYRVIEVNARLSRSSALASKATGYPLAFVAAKLGLGYGLFDLKNSV
                     TKDTTAFFEPALDYVVCKIPRWDLGKFHGVSRELGSSMKSVGEIMAIGRSFEEVIQKG
                     LRMIGQGMHGFVANKELVISDIDKALHEPTDNRIFVISKALRKGYTVDEIHRLTMIDK
                     WFLCKLNNIVQTANEFETLHSFHKITPEMMKTAKQQGFSDFQIARLAGNNETDLEHAL
                     LEIRKIRKSMSIVPVVKQIDTLAAEHPAQTNYLYLTYNGCTNDVKYLGDKKSVVVLGS
                     GAYRIGSSVE"
BASE COUNT         1375 a          726 c          734 g         1271 t
ORIGIN      
        1 atcggatgcc tgtatctaaa ctctgaagca tattcaactt ctacaggtat cctacataaa
       61 tcttctaaaa gaaactctcc tattagtgcc gcatgccaag atgtaccaca cgcaacaata
      121 ataatacgct tagcattaag aaatttatct ttattatcta caactcctga taaaacgact
      181 tctgttcctt caacatttat acgtcctcta atacaatcaa ttaaggtttg ttgctgttcg
      241 tgaatctctt tcaacataaa attttcatag ccaccctttt caagttgact tagattcatc
      301 tttaatgttg taacttctgc tgtttgctca acatcagaca gatttaataa cttaacacca
      361 ttttttctgt caattactgc aacttcttca tcattaacat atacaacctt atctgtgtat
      421 tctacaatag gtgtggcatc agacgctaaa aaatattcgt ttttacctat gcctacgact
      481 aagggactac tttttcttgc ggctactatc tgatttggat tttctttatc aataacagct
      541 atggcataag cacctattac ttgtgaaagg gctatttgca ctgcatgaca cagatctacc
      601 ttgtttgttt tttttacata ttcaataaat tgaactaaaa cctcagtatc ggtagtactt
      661 ttaaatgtat atccttggcg taacagctct ttttttatta cagaatagtt ctcaataatt
      721 ccgttgtgaa tcaaagcaat tgtttcggac tgcgagtagt gcggatgagc attctcattg
      781 ttaggttcgc catgagttgc ccaacgtgta tgagcaatac ctatagtacc tgatacatct
      841 tgcgactcaa ccaaagattc caaatcagaa acctttcctt ttgttttgta aacattaagc
      901 tcctttttat cattcataaa agccacacct gcactatcgt atcccctata ctccaaacga
      961 tgcagaccct taatcaaaat aggataagct tgctgattac cgatataccc gacaatacca
     1021 cacataattg tttttttata taataattct ttacaaaagt accatactaa tagccataat
     1081 ttaatgacta ctacattaaa aggatttgta tttttatgca aaatttcaaa ttatcaataa
     1141 aacgacacta aaaaagtaat tccgtaacca aaaacaactt aatttataac aaattatcaa
     1201 actgtattat aaaaatataa cacaaacagc ttttttgatt atgcgttttg aaaaacaact
     1261 aactttgcaa tgtattaata acgattacta tgcgactttc tctacctgca tcactaatat
     1321 tggacaatgg ttctgtattc cgcggatatt cattcgggta caaaaaatca accgccggtg
     1381 aggttgtttt caacactgca atgtcagggt atcctgaaag tcttactgac ccatcatact
     1441 caggtcaaat cttaactttt acatttcctc ttattggaaa ttatggagtt ccttccacaa
     1501 atatttcgaa tggcatttca gattttcttg aatcagaaaa gatacatact tcgggaattg
     1561 ttgtgtccga ttattcattt gaatattcac attggaatgc aaaacaaagt ttacacgatt
     1621 ggcttgagtc taataaaatt cccggcattt acggcataga tactcgcgag ttaaccaaaa
     1681 tattacgtaa cgaaggatct atgaaaggca aaatcatcat tgatgattat aaggatatcg
     1741 attttattga tcccaatata gaaaatcagg ttgcaaaggt atcgtgcaaa gaagttattg
     1801 aatacaacaa ttcaggaaag acaattgtga tgatagattg cggagttaaa cataatatta
     1861 tacgatgttt attaaaacgt ggggttagac taatccgagt accttgggat tacgactaca
     1921 attcgatgca atttgatgga ctatttattt caaatggacc cggcgatcca aatacatgtg
     1981 gcaagacaat tgcaaacatt caaaagacta tgcaaagcgg taaaccgatt tttggaattt
     2041 gtatgggaaa tcaattattg tgtaaagcag gaggatccga aacttacaaa ttaaaatacg
     2101 gacatagaag tcataaccaa ccggttagag aagttggaac taatcgttgt tttataagtt
     2161 cacaaaatca cggatacgct gtcgataata atactctaag taaagattgg gaagttctgt
     2221 ttgagaattt gaacgatcat acaaatgaag gaataagaca taagacgatg ccctggtttt
     2281 cggctcaatt ccatcccgaa gcagcaagtg gtcctacaga tactgagttt ttattcgatg
     2341 attttctaca aatgataaag taaagccatg tcaaacatga tctcaaacat aaaaaaagta
     2401 ttagtattag gctccggtgc actaaagata ggtgaggcag gagagtttga ttactcaggt
     2461 tcacaggctc ttaaggcatt gaaagaagaa ggtatcttta ctgtacttat aaacccaaat
     2521 attgcaactg ttcaaacatc tgcagatatt gcagaccaaa tatacttttt accggttaca
     2581 ccattctttg tcgaaaaaat tattcaaaaa gaaaaaccgg aaggaatatt acttgccttt
     2641 ggtggacaga cagctctaaa ctgtggagtt gaactttaca aagccggtat tttagaaaaa
     2701 tatggcattc aggtattagg aactcccgta caagcaatta ttaatacaga agatagagaa
     2761 ttgtttgtta agaagttaaa tgagattaat gttaaaacga taaatagtga ggcggtcgaa
     2821 aatgctgagg atgcacgtaa ggctgcagaa aggataggat accctgttat tataagagct
     2881 gcttatgccc tgggtggact tggaagcgga ttttgtaata acgaatcgga attagatgta
     2941 atggtagaaa aagcgctatc attttcacca caagttttgg tagaaaaatc tcttaaaggt
     3001 tggaaagaag ttgaatatga agtagtaaga gatcgattcg acaattgtgt gacagtttgc
     3061 aacatggaaa atttcgaccc tctaggtata cacaccggtg aaagcatagt tatagctcct
     3121 tctcaaactt taagcaatag tgaataccat aaacttcgcg cacttgcaat taaaataatc
     3181 agacatattg gtattgtcgg agaatgtaat gtacagtatg cgcttgaccc ggaatcagaa
     3241 gattatcgcg ttatcgaagt taatgctcgt ctaagtaggt cttctgcttt ggcatcaaaa
     3301 gcaaccggat atccattagc atttgttgct gcaaaattag gtttaggcta tggcttattt
     3361 gatttaaaaa attcagttac aaaagatact actgcatttt ttgaacctgc acttgattat
     3421 gtggtatgta aaattcctcg ttgggattta ggcaagttcc acggggttag ccgtgagctt
     3481 ggttcaagca tgaaatctgt aggcgaaata atggctatag gacgcagctt tgaagaagtt
     3541 attcaaaaag ggttacgaat gataggtcag ggaatgcatg gttttgttgc taataaagaa
     3601 ttagttattt ctgatataga caaagccctg cacgaaccta cagataatcg tatttttgta
     3661 atttcaaagg ccctgcgtaa aggttatacg gttgatgaaa tacaccgtct aaccatgatt
     3721 gacaaatggt ttttgtgcaa actgaataat attgtacaaa cagccaacga atttgaaact
     3781 ttgcatagtt ttcataagat cactcctgaa atgatgaaaa cagctaaaca acagggtttt
     3841 tctgattttc agatcgcgcg tttagccggt aataatgaaa cagacttaga gcatgctctt
     3901 ctcgaaataa gaaagattag aaaatcaatg agtattgtac ctgttgtaaa acagatagat
     3961 acgcttgctg ctgaacatcc ggcccaaaca aactatttat acctgaccta taacggctgc
     4021 accaatgatg ttaaatacct aggcgacaaa aagtctgtag ttgttctcgg ttcaggcgca
     4081 tatcgcatcg gtagttcggt tgaatt
//
LOCUS       DAOPID010000138         4064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_29951_length_4064_cov_4.529558, whole genome shotgun sequence.
ACCESSION   DAOPID010000138 DAOPID010000000
VERSION     DAOPID010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29951_length_4064_cov_4.529558"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PK641_05205"
     CDS             <1..431
                     /locus_tag="PK641_05205"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743774.1"
                     /note="catalyzes the formation of oxaloacetate from
                     L-aspartate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HPG55779.1"
                     /translation="GANRLASNSLIEAIVYADAAAQEALKNVDNIPLNEEVPMWNDEG
                     TSLNEEMVLITQSEKEVGQIMSNYVGIVRSNLRLKRAFDRLEILYKETEDLFDRSVVT
                     KNICELRNIINTAYLVIKMAMERKESRGLHYTIDYPDKID"
     gene            545..1744
                     /locus_tag="PK641_05210"
     CDS             545..1744
                     /locus_tag="PK641_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006848400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPG55780.1"
                     /translation="MEIIERNNYLNKLLALKDKQIIKIVTGIRRCGKSTLLEMYQHRL
                     LLNGVESNQIIAVNFESFDFLELRDPKKLYEYINSRISVTKMNYIFLDEIQHVYKFPD
                     VVNSLFIKKNVDLYITGSNAYMLSSEIATLISGRYVEIPMLPLSFKEYVQSTGDMRDL
                     SLKYANYTRYGGFPYSLELRDQPQELNDYLRGIYSTIVLKDVVDRKKVSDVMMLENVM
                     RFAFDNIGNILSTKRIADTMTSNGHKIDVKTVEKYLSALIESFVLYQVKRYNIKGKQY
                     LKTLEKYYVVDMGLRSMLLGSASFDVGHILENVVYLELLRRGRQMYVGKVDELEVDFV
                     ATDADSLCYYQVAATVREPSTLLRELAPLQNINDNNPKYLLSLDDESNISHNGIMQIN
                     VLEWLLQ"
     gene            complement(1846..2817)
                     /locus_tag="PK641_05215"
     CDS             complement(1846..2817)
                     /locus_tag="PK641_05215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HPG55781.1"
                     /translation="MKKYLAAIIVLLILSVGKLSAQEELIYNQYHFNYYLVNPAMAGA
                     EPCSHLMISNKMQWVGMEDFPMTQLLSFKTRVWKNVGIGAYLYNDRNGYSNRAGGQVT
                     FAYHIPLSKGRSYSKKVSYDRQLSFGASFKANYFYINTDKLFADDPDAQYDNAFANTS
                     GFGVNANFGIYYKSYGGFVGLSLTNLIPMTADIYGDKEMPAPLTGFLFGGYTFDLNRR
                     GDYYIEPMVMLKMNQYLDINMDLNLKFGQEVSKDWGYWVQASYRHSWDANNIQSLVLM
                     PMCGFRIHGFQLGYAFSLDLNNLVTQNYGTHEIMLGYSFCYTKHFCR"
     gene            complement(2868..>4064)
                     /locus_tag="PK641_05220"
     CDS             complement(2868..>4064)
                     /locus_tag="PK641_05220"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HPG55782.1"
                     /translation="QINITDSDPNTATSYVWMQEISPYAYQEISQQTAKDISGFTPTV
                     TANYKKISYNGVCSAESNEVTVTVHPAIVFSIEPIDRTICEGEDVTITMSGYPTGSTT
                     EWFEKATTSLISSDPSTVVTPSQTTVYTARVTNICTAEKDLTVSVLPPINPQISQPTA
                     ICLGDSTILTVSGNGIVSYSWVPSGSLDSPSAATTIARPKTNTQYIVTVSNGVCDVTA
                     TTEVTVNALPLFDTIEEQNTENCSTRSVTVTAKEGTPPYQFSEDGDTFTTQNVFTGMT
                     SGWNILFVIDAYTCKGDTSFYLEPYPIIPDRFVTPNNDNTNELWTVENLNCYSAYIVE
                     IFDRYGKRLYEWRKGSFSKSANPEDFPGWDGMYNGHQLPSTDYWYLITVEEIRKQYNG
                     NFILKR"
BASE COUNT         1270 a          649 c          769 g         1376 t
ORIGIN      
        1 acggagctaa tcgtttggca tcaaattctt taattgaggc tattgtatat gccgatgcag
       61 ctgcgcagga agcacttaaa aatgtagata atataccatt aaacgaagag gttccgatgt
      121 ggaatgatga gggtacttct ttaaatgaag aaatggtttt aattactcaa agtgaaaaag
      181 aagtaggtca gataatgagt aattatgttg gtattgttag atctaattta cgtttaaaac
      241 gtgcctttga tcgtcttgag attttgtaca aagaaaccga agatttattt gaccgttctg
      301 ttgtaactaa aaatatttgc gaattgcgta atattattaa taccgcttat ttagttatta
      361 agatggcaat ggagcgaaaa gagagtagag gtttgcatta tacaattgat tatcctgaca
      421 aaatagatta aaatcatatt tataagacaa gttttgggat tgtaatccaa aaagtatcta
      481 aataagtaaa gtttttggat tgtattccaa aaactttact tatttttgta caaaatatgt
      541 ttttatggag ataatagaac gcaataacta tttgaataaa ttgcttgctt taaaagataa
      601 gcagattatt aagattgtaa cgggtatacg ccgttgcggt aaatctactc tgttagaaat
      661 gtaccagcat cggctattgt tgaatggtgt tgagagtaat cagattatag ctgtgaactt
      721 cgagagtttt gattttctcg aattgcgtga tccaaaaaaa ttatacgaat atataaattc
      781 gcgcatatct gttaccaaaa tgaattacat ttttttggat gagattcagc acgtatataa
      841 attccctgat gtggtgaata gcctttttat aaaaaagaac gtagatttat atattacagg
      901 ctcaaatgct tatatgcttt cgagtgagat agcgactctt atatccggca gatatgttga
      961 gatacctatg ttgccattat cgtttaaaga atatgtgcaa agtacaggtg atatgcgtga
     1021 tttgtcttta aagtatgcca actataccag atatggcggt ttcccttatt ctttagagtt
     1081 acgagaccaa ccacaagaac ttaacgatta tttaagaggg atatatagta ccatcgtttt
     1141 aaaagatgta gtagatcgta agaaagtatc ggacgtaatg atgctcgaaa atgtaatgcg
     1201 ctttgccttt gataatattg gcaatatttt atcgacaaaa agaatagcag atacaatgac
     1261 aagcaatggg cataagattg atgtaaaaac agttgagaaa tatttatctg cattaataga
     1321 gagttttgtt ttgtatcagg taaaacgtta taacataaaa gggaaacaat atcttaaaac
     1381 cctcgaaaaa tattatgtag tagatatggg cttaagaagt atgcttttag gatctgcttc
     1441 gtttgatgtg ggtcatatct tagagaatgt tgtttatttg gaattgttaa ggcgagggag
     1501 gcagatgtat gtaggaaaag ttgatgaatt ggaggttgat tttgtggcta cggatgctga
     1561 ttctttatgc tattatcagg ttgccgcaac agttcgcgag ccttccacac tattgcgtga
     1621 gctagctcca ttacagaata taaatgataa taatccgaag tatctgctaa gcttagatga
     1681 tgagtcaaat atctcgcata atggtattat gcagattaat gttctggagt ggttattgca
     1741 ataagttttg ggattatatt cccaaaagta tctaaataag taaagttttt ggattgtatt
     1801 ccaaaaactt tactttgcga aagaactata tatatgtgtt gttgcttaac ggcagaagtg
     1861 tttagtgtag cagaaactgt atccaagcat tatttcatga gtaccatagt tctgtgttac
     1921 caaattgttt aggtctaaac taaatgcata acctaattgg aagccatgta tacggaaacc
     1981 acacataggc attaaaacta aactttgtat attgtttgca tcccaactat gtctgtatga
     2041 tgcctgtacc cagtatcccc aatcctttga aacttcctgt ccaaacttca agttcaagtc
     2101 catgttgata tccaagtatt ggttcatttt aagcatcacc ataggttcaa tataataatc
     2161 tccacgtctg tttaggtcga atgtataacc accaaataag aatccggtta atggagcagg
     2221 catctctttg tctccatata tatcagcagt cattggaata aggtttgtta aagataatcc
     2281 aacaaagccg ccataagatt tataataaat accaaagtta gcatttacac caaaacctga
     2341 tgtgttagca aatgcattgt cgtattgcgc atcaggatca tcagcaaata atttgtctgt
     2401 gttaatgtaa aaatagtttg ccttgaatga tgcaccaaaa gataattgac gatcgtagga
     2461 gactttttta gaataactac ggccttttga tagtggaata tgatatgcaa atgttacttg
     2521 tccacctgcg cgattagaat aaccattgcg atcattgtac aaataagcac caataccaac
     2581 attcttccat acacgagttt taaatgatag taattgtgtc attggaaaat cttccattcc
     2641 aacccattgc attttgttac ttatcattaa atgtgagcat ggctctgcac ctgccatggc
     2701 gggattcacc aaataatagt tgaaatgata ttgattatat atcaattctt cttgggctga
     2761 taatttgcca acgctaagta ttaataatac gattattgca gcgaggtact ttttcatgat
     2821 atgttagttt ttctatttcc taaatttatg ctcaaatata caattattta tcgtttaagt
     2881 atgaaattcc cgttatattg tttacgaatc tcctcaactg taataagata ccaataatca
     2941 gtacttggta attgatgtcc gttatacatg ccgtcccatc cggggaaatc ttcgggatta
     3001 gctgatttgc taaaacttcc tttacgccat tcgtaaagac gttttccgta tctgtcaaat
     3061 atttctacta tgtatgcaga gtagcagttt aagttttcta ctgtccatag ttcgtttgta
     3121 ttgtcgttat taggcgttac aaaacgatcc ggaataattg gataaggttc taagtaaaaa
     3181 cttgtatcac ccttacatgt atatgcatca ataacaaata atatattcca accgcttgtc
     3241 atacctgtaa atacattttg tgttgtgaag gtatcgccat cttcagagaa ctgatatggg
     3301 ggagttcctt ctttagctgt aacagtaacg ctacgagttg aacaattttc tgtgttttgc
     3361 tcttcaattg tatcgaataa tggtaatgcg ttaacagtta cttctgtagt cgctgttaca
     3421 tcacagactc cattactcac tgttactata tattgagtgt ttgtttttgg ccgtgcaatt
     3481 gtagtcgcag cactaggact atctagtgag cctgaaggaa cccaactata tgagacgatg
     3541 ccattaccac taactgtcaa tatcgtgcta tctcccaaac aaattgctgt tggctgactt
     3601 atttgaggat ttataggagg cagtacgcta acagttaaat ctttttctgc agtacagata
     3661 ttggtaactc gagctgtata tacagttgtc tgcgaaggtg ttactacagt acttggatcg
     3721 cttgaaatta agcttgttgt agctttttca aaccattctg ttgtcgatcc ggtaggatag
     3781 cctgacattg tgattgtaac atcttcacct tcacatattg ttctgtcaat aggttcgata
     3841 ctaaatacaa ttgcaggatg aacagtcaca gtaacttcat tactttctgc actacacact
     3901 ccattataag atattttctt gtaattggca gttactgtag gcgtaaatcc gcttatgtct
     3961 ttagctgttt gctgcgaaat ctcttgatag gcgtatgggc ttatttcttg catccaaaca
     4021 tagctggttg cagtattcgg gtcgctatcg gtaatgttta tttg
//
LOCUS       DAOPID010000139         4058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_30011_length_4058_cov_3.829378, whole genome shotgun sequence.
ACCESSION   DAOPID010000139 DAOPID010000000
VERSION     DAOPID010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30011_length_4058_cov_3.829378"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="PK641_05225"
     CDS             complement(<1..881)
                     /locus_tag="PK641_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPG55783.1"
                     /translation="MSKIVETPMLKQYNAVKKQHPDALLLYRCGDFYETYGEDAVTAS
                     AILGITLTMRGKDTSNEIRMAGFPYHAIDTYLPKLVRAGWRVAICDQLEDPKLAKGIV
                     KRGITELVTPGVSYNDNVLENKSNNFVACLHFGKSAIGLAFLDISTGEFLLTQGTAEY
                     VSKLIANFAPKEVLYEYGKKQKWEDLSAGKYFTFQLDDWMFTTDAANNRLKKQFDVLT
                     LKGFGVDDLPEGVIAAGGILHYLDATQHLQTAHITNLKRIEGDSYVWMDKFTIRNLEL
                     YGSMNGEGKSFISILDKT"
     gene            1122..>4058
                     /locus_tag="PK641_05230"
     CDS             1122..>4058
                     /locus_tag="PK641_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55784.1"
                     /translation="MQKKVFFILISTLFALFGGVSLYAQSCTTQFGGTTFQPKSGVTA
                     TYYDLTDVSFVDPTSEYITVGSGYSFQNTSPASLATSRYTISETDGLVLHGRELGQYG
                     FFSYQLSSLLTAAGRNSYKVEIVFSAAQQTGCSTSIEGWNNYIKMKCSPAADAEYKTI
                     NFGTTYTYSYTFTAASSSQTFAFLSDYSNGCGLLSVKSIRVTGCFDKAIVSSNGNKVC
                     AGELNTLTAKGLTGGSYTWEQSIDNKFTWQPLVGNQQSINVDVKTDTYYRVTSGSETL
                     VSSLIRPIVCCAVSGDRQTMSAEEFTFTGPRTSNSSCQYTYKSSGNINEGQYAVVHSA
                     ADGGHWTNRKLKGHTTNGLQPDDGFLLVNAARAAGMFYEQYINNQLCQNTMYDFSAFI
                     ANACPDDGKAGRVPVNAGFKVYGCTSPGNCPDELLSVESGNLPDGTDWTEKGGSFNSG
                     SYTSVNIQVFNNINAPGTGTVDGNDIAIDDITFSTCSPEIKMYSNSELTNQDTTVCDY
                     GNNVTLNLIAAAVYDMTDFFTTPYYLMQQSNTPTGPWTQVGVSQTSNTFAITVPATQS
                     APIYYRTWVAGDQTTVEKVSQGQPVSGCGSVSAVTDPISVRYHCGCSPTTPPTVTAYS
                     QCPCQTGTSLLADLVTSDKTQLRFYSDATGGTALPATKTFDCRTVHDTVYYVTNQFAT
                     VGTTEYCESDRIPININIKDAVQFTISPKDITTCLTSGVNLTYTVSNVVPAGAQITWK
                     TSPDITGTTYTLSATEGSGTINIEATDASACLATDQVTYQLLNTPDFTLSAPTMVCVS
                     NPVAEIKAQVSAGSGSYQWFKNGVEVANGTMTLGQTEITYNDPAVASAEGSVTYRLKL
                     DNGICFTEKTVSVSVGDRIDIPITASAPAQNNTICLGATIDLTAGYTLNAGESLTWTV
                     GGVVVPETGTVLASQQPTANTTYLVSLVGGICEGDGSITINVDTPPAPAITVDKDVIC
                     EGTQINI"
BASE COUNT         1342 a          799 c          729 g         1188 t
ORIGIN      
        1 gtcttgtcta aaatgctgat gaacgattta ccctcgccat tcatcgaacc atatagctca
       61 agatttcgaa ttgtaaattt atccatccaa acataagaat caccctcaat acgtttaaga
      121 tttgtgatat gagccgtttg caagtgctgt gtagcatcca aataatgtaa aattccaccg
      181 gctgcaatca caccttcagg caaatcatca actccaaaac cttttagagt taaaacatca
      241 aattgtttct ttaaacgatt atttgcagca tctgttgtaa acatccaatc gtcaagttgg
      301 aaggtaaaat atttgcctgc cgacaaatct tcccatttct gttttttgcc atattcgtac
      361 aaaacttctt ttggagcaaa gttagctatc aacttactta catattctgc ggtaccttgt
      421 gtaagaagaa attcgcctgt agaaatatcc aaaaaagcta atcctatggc tgattttcca
      481 aaatgcaaac aagccacaaa attgttgctt ttattttcaa gcacattgtc attatacgaa
      541 acgcccggtg taactaattc tgtaatacca cgttttacaa ttcctttagc tagtttagga
      601 tcttctaatt gatcacaaat tgctactcgc caacctgcgc gtactaattt tggaagatat
      661 gtatcaattg catgatatgg gaatcctgcc attcgaatct catttgaagt atctttcccc
      721 ctcatcgtaa gagtgatacc taaaatagca gatgcagtaa ccgcatcttc tccgtatgtt
      781 tcataaaaat ctccacaacg ataaagcaac aatgcatcag gatgctgttt ttttactgca
      841 ttgtactgct ttagcatggg agtttcaaca atctttgaca taagtaaaac aaaataattt
      901 tcattgagca gcaaatatac atctaattca acacttaata ctatgtgcag agtccataaa
      961 tatttaagaa tttgcatctt tttataaaat aaatgcatct tttctctcag aaatacaata
     1021 attattaagc tttttaggtt aattcattaa cggatatttt atttttagaa taaatttttt
     1081 acttttgtga gttgaattat gtttacctta aaaccaaaac tatgcaaaag aaagtattct
     1141 ttatattgat ttctacatta tttgccctat ttggaggagt aagtttgtat gcccaaagtt
     1201 gtacaactca attcggaggt acaacttttc aacccaagtc aggagttacg gcaacgtact
     1261 atgacctaac tgacgtatcc tttgttgatc ctacttcaga atacattaca gttggatcgg
     1321 gatatagttt tcaaaacaca tctcccgcat cactagccac aagccgttat acaataagtg
     1381 aaacagatgg tttggtatta cacggtcgcg aactaggtca atacggattc ttttcgtatc
     1441 aactaagcag tttattaact gcagccggta ggaattcgta caaggttgag attgtatttt
     1501 ctgctgcaca acaaaccgga tgcagtacat caatagaggg ttggaacaac tacattaaaa
     1561 tgaagtgttc gccggcagct gatgcagaat acaaaacaat taacttcgga acgacataca
     1621 cttattcgta cactttcaca gcagcatcaa gttcacaaac tttcgcattt ttatccgact
     1681 actcaaacgg atgcggatta ctttcagtaa aatcgatacg agtaacagga tgttttgaca
     1741 aagcaattgt ttcgtcaaat ggaaataaag tttgcgccgg agaattaaac actcttacag
     1801 caaaaggttt aacaggaggt tcatacactt gggaacagtc tattgacaat aaatttacat
     1861 ggcaaccact tgttggaaat caacaaagca taaatgttga tgtaaaaacc gatacatact
     1921 acagagttac gtcaggtagt gaaacacttg tatcttcttt aatcagacct atagtatgtt
     1981 gcgcagtatc aggagataga caaaccatgt cggcagagga atttacattt acgggaccaa
     2041 gaacaagtaa ttcttcatgt caatacacat acaaatcatc gggcaatata aatgaaggtc
     2101 aatatgccgt ggtacactca gctgccgatg gcggacattg gaccaacaga aaattaaaag
     2161 gacatacaac aaatgggtta caacctgatg atggttttct attggtaaac gctgccagag
     2221 ctgccggtat gttttacgaa caatacataa acaatcaact ttgtcaaaat acaatgtatg
     2281 atttttcagc atttattgca aatgcatgtc ctgatgatgg gaaggcaggc cgtgtacccg
     2341 taaatgcagg ttttaaagta tatggatgta catctcccgg aaattgtccg gatgaattat
     2401 tgagtgttga atcaggaaat ttacctgatg gcactgattg gaccgagaaa ggtggttctt
     2461 ttaattccgg ctcatacaca tctgttaata tacaggtatt taacaatatt aatgctccgg
     2521 gaacaggaac tgtagatgga aatgatatcg ctattgatga tattactttc agtacttgtt
     2581 cgcccgaaat caagatgtac tcaaattcag aattaacaaa tcaagataca actgtttgtg
     2641 attatggtaa taatgtaaca ttaaatttaa ttgcagctgc ggtatacgac atgaccgact
     2701 tctttacaac cccatactat ttgatgcaac aatctaatac tccaacaggt ccttggacac
     2761 aagttggagt atctcaaaca tcaaatacat ttgcaataac tgttcctgca actcaatctg
     2821 ctccaatata ttatcgcaca tgggttgcag gtgaccaaac aaccgtggag aaagtttcac
     2881 aaggccagcc tgttagcggt tgcggtagtg tgtctgcagt tacagaccct atatcggtaa
     2941 gatatcattg cggttgtagc cccacaactc caccaacagt tactgcttat agccaatgtc
     3001 cttgtcaaac aggaacttct ttacttgcag acttggttac atcagataaa acacaattac
     3061 gcttttattc tgatgcaaca ggtggtactg ctttgcctgc aacaaaaaca tttgattgta
     3121 gaacagtaca cgacacagta tattatgtaa ccaatcaatt cgcaacagta ggaacaaccg
     3181 aatactgcga gagcgaccgt attcctatca acataaatat taaagatgca gttcagttta
     3241 ccatatcacc aaaagatatt actacatgtt taaccagtgg tgtaaacctt acttatactg
     3301 ttagtaatgt agtacctgca ggtgcacaaa taacatggaa aacatctccc gatatcaccg
     3361 gaacaacata tacattgagt gccactgaag gtagcggaac tattaatatt gaagctacag
     3421 atgcaagcgc atgtcttgca acagatcaag taacatacca attattaaac actcctgact
     3481 ttacattatc tgcaccaaca atggtctgcg tatctaatcc ggttgctgaa attaaagcac
     3541 aagtaagcgc aggatccggc agctatcaat ggttcaaaaa cggtgtagaa gttgcaaacg
     3601 gaacaatgac gctgggacaa acagaaataa catataatga ccctgctgtt gcaagtgcag
     3661 aaggttctgt tacatataga cttaaattag acaatggtat atgctttaca gaaaagacag
     3721 tatctgtaag tgtaggcgac agaatagata ttcctattac tgcaagtgca cctgcacaaa
     3781 ataatactat atgccttggt gctactattg atttaacagc aggctacaca ctaaacgcag
     3841 gcgaaagtct tacatggact gtaggaggtg tggtagtacc cgaaaccggc accgttttag
     3901 cttcgcaaca acctactgct aatacaacat accttgtaag tttggtaggt ggaatatgcg
     3961 aaggagatgg aagtattaca attaatgtag atactcctcc ggctcctgcc attacggttg
     4021 acaaggatgt gatttgcgaa ggtactcaaa taaacatt
//
LOCUS       DAOPID010000140         4055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_30039_length_4055_cov_4.314750, whole genome shotgun sequence.
ACCESSION   DAOPID010000140 DAOPID010000000
VERSION     DAOPID010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30039_length_4055_cov_4.314750"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..1596
                     /gene="cls"
                     /locus_tag="PK641_05235"
     CDS             163..1596
                     /gene="cls"
                     /locus_tag="PK641_05235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HPG55785.1"
                     /translation="MDLLQVLYYVLLAAYWILVISSVSVVLLENKNPVRTTAWILVLT
                     MVPFIGLLFYIVFGRSYHRQSRLSRKTKAKIQVCMNPAIARTELATNSKIVKKHAHLI
                     KLLETSSEAKLFANNTVEIYTNGKKLFADILNDIENAKNHIHAEYYIVETDKTGNKFK
                     DALIKKAREGVEVRLIYDDIGSWRLKYFTIKEMQDAGIKLQSFFKLRFPYFTNKLNYR
                     NHRKILVIDGKIGYLGGFNIADRYTDGLDWGIWKDTHIRFEGDGVAGLQNIYLTDWLY
                     VTQKLHQTPRFFPISSCESKTAIQVVGSGPDTDRQSIMQGFCFAINDAKKYIYIQTPY
                     FLPNESILTAIESAAMRGIDVRLMIPFRSDSRISYEASLSYMGEILSAGVKVIQYRKG
                     FIHAKTIVIDDEVAIIGSANMDFRSFEQNFEVSAFIYDEEKSVELRNIFLVDEKSCRK
                     LTLWKLSKTPKFRKVIRSIARLFSPAL"
     gene            1593..2105
                     /locus_tag="PK641_05240"
     CDS             1593..2105
                     /locus_tag="PK641_05240"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HPG55786.1"
                     /translation="MIRIFLIGFMGAGKTTFGKQLASNLGLSFIDTDVYIENRYHKTV
                     SSLFAEKGESEFRILERKILEEISKIEDVVIATGGGMPCFYDNIDYMNSKGITIFMQV
                     SDSELAKRLYPAKSNRPLLMDKDYDEILSFVSSMLTKRLPFYLKAQYTVDASGNKVVD
                     RMAEICTKID"
     gene            complement(2102..3808)
                     /locus_tag="PK641_05245"
     CDS             complement(2102..3808)
                     /locus_tag="PK641_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55787.1"
                     /translation="MKPYLTINMKALKLITCLCLIASIPAYGQKAVSETESITRNVTV
                     EKAYDPTTIEVTKISPVPPKEEYTPEQPSVTYSTWSKAEEVSTTPDKLKAEQYENPET
                     ADTKNGIFKAGLGFYWQVLGEFYYPILRGDKYLLDVNLKHLSNWSKIRLEDGSVPRAM
                     TQNTSANVTFESQFRDSRLNSALDFSYNGFDYYGKSTVPVINDPIKDTIGTYTTVGVN
                     FNLFSTNTKKAFQYNTTLGYNYLGRNFGLSNHNILVKADLGGEVGNGELGADIDIDTN
                     IKGLTEESTNTYKSEAGCLISIMPYYKLIGKTWDLKIGGNLYILAADSTKRPVTGSAN
                     IEGRFGLVPGLFHLYTGIKGSFVENTYPNIIKENPYIKPLLSLEPTYTPLNIALGMKV
                     NIMQGLLFDLGFDYSLILDQYYYVNDTINGNYYNTFNVTYENTTNKVSVQAGLYFDYV
                     KNLNISLVGKYNHWGVTDNEFAWQKPAWEIKLEASYIIKEKWQIGLSYNFLGERYARV
                     GTEAIRMTDIHDLNVYVSYKALDWLSVFANGKNLINIQADKYYGYRNFGINGMIGATF
                     SF"
     gene            complement(3786..>4055)
                     /locus_tag="PK641_05250"
     CDS             complement(3786..>4055)
                     /locus_tag="PK641_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55788.1"
                     /translation="TYKINSQNDEAEKEIFDFIDKGTPHTYWLAKSFILLSDIYIEQE
                     NYFEAKQYLLSLQDNYKEADEDIKKAIAERLEIIEKHENETVSNN"
BASE COUNT         1273 a          621 c          724 g         1437 t
ORIGIN      
        1 tttggattgt tttatcagat ctaattttta tgatgaggta caaatagagg aatctcttgt
       61 taaatgtggt tctgggattg ctgctccaat tataaaaatt cctgaggatg ctgatattaa
      121 aatgtatgga aatcacagaa taatcagttt taaatcaaaa taatggattt attacaagtt
      181 ttatattatg tgttgcttgc tgcctattgg attttggtaa tttcaagtgt ttcagtagtg
      241 ctgcttgaaa ataaaaatcc ggtaaggaca actgcatgga ttttggtatt gacgatggtt
      301 cctttcattg gattattgtt ttatattgtt tttggtcgta gttatcacag acaaagcaga
      361 ttgtcacgca aaacaaaagc aaagatccaa gtttgtatga atcctgcaat agctagaact
      421 gaattggcaa caaatagtaa gatagtaaaa aaacatgctc atttaataaa actattagag
      481 acttcttctg aagcaaagtt atttgcaaat aatactgttg agatatatac aaatggtaaa
      541 aaactatttg cagatatcct taatgatata gaaaatgcta agaatcatat tcatgcagaa
      601 tattatatag ttgaaacaga taagactggt aataagttta aagatgcttt aattaagaaa
      661 gctcgtgagg gtgtagaagt cagacttatt tacgatgata taggtagttg gaggcttaag
      721 tattttacaa ttaaggaaat gcaggatgcc ggaataaagt tacagtcatt ctttaaactt
      781 aggtttccat attttacaaa caagttaaat taccggaatc accgaaagat tctagtaatt
      841 gatggcaaaa taggttatct tggaggattc aatatagctg ataggtatac tgacggactt
      901 gattggggaa tttggaaaga tacacatatt cgatttgaag gtgatggtgt tgccggactg
      961 cagaacatat atcttacaga ttggttgtat gttacacaga aattacatca aacacctaga
     1021 tttttcccca tttcatcttg cgaaagtaaa actgcaatac aagtagtggg tagtggacct
     1081 gatacagata gacaaagcat aatgcaaggc ttttgttttg caattaatga tgctaaaaaa
     1141 tatatctata ttcaaactcc atattttttg ccgaacgaaa gtatattaac agccatagaa
     1201 tctgcagcaa tgcggggtat agacgttcgt ttgatgatac catttcgttc ggattctcga
     1261 atatcatacg aagcatctct atcatatatg ggagaaatat tgagtgccgg tgttaaagtt
     1321 attcagtata ggaaaggttt tattcatgcg aagactatag taattgatga tgaagttgct
     1381 attataggtt ccgcaaatat ggattttcgt agtttcgaac agaattttga agtcagtgct
     1441 tttatttatg atgaagagaa gagtgtcgaa ttgcggaata tatttcttgt cgatgaaaaa
     1501 tcttgccgaa aattaacact atggaagttg agtaaaacac ctaaattccg taaagtgatt
     1561 agatcaatag cacgtttatt tagtcctgcc ttatgatacg tatatttttg ataggtttta
     1621 tgggggccgg aaagactact tttggcaagc aattagcttc taatttagga ttgtctttta
     1681 ttgatactga tgtttatatt gagaataggt atcataagac tgtttcatca ttgtttgctg
     1741 aaaaaggaga gtcggagttt cgtatattgg aacgtaaaat attagaagaa atatctaaaa
     1801 tcgaagatgt tgttatagca acaggaggcg gaatgccttg tttttatgat aatatagatt
     1861 acatgaatag taagggcatt actattttta tgcaagtctc cgattcggaa cttgctaaac
     1921 gcttatatcc ggcaaaatct aataggccac ttcttatgga taaggactat gatgaaatac
     1981 tttcttttgt aagttcaatg cttacaaaga gattgccatt ctacttaaaa gcccaatata
     2041 ctgttgatgc ttcaggcaat aaagttgtcg atagaatggc tgaaatttgt actaagatcg
     2101 attaaaaaga gaatgtggca ccaatcattc catttatgcc aaagttccta tatccgtagt
     2161 atttatcagc ttgaatattt attagattct ttccattagc aaatacactc aaccaatcta
     2221 atgctttgta tgaaacataa acattaagat cgtgtatgtc tgtcattcga atcgcttctg
     2281 ttcctactcg agcgtatctt tcgccaagga agttataaga caatccgatt tgccattttt
     2341 cttttataat atagcttgct tccaatttaa tctcccacgc cggtttttgc catgcgaatt
     2401 cattatcagt tacaccccaa tgattgtact ttccgactaa acttatattt agattcttta
     2461 cataatcgaa atataatccg gcctgaactg atactttatt ggtcgtattt tcgtaagtaa
     2521 cattaaaagt attatagtaa ttgccgttta tagtatcatt tacgtaataa tattgatcta
     2581 atataagact atagtcaaat cctaaatcaa aaagaagccc ttgcataata tttactttca
     2641 ttcccaaagc aatatttagt ggcgtatatg ttggctccaa acttaaaaga ggtttgatat
     2701 atgggttttc tttaattatg tttggatatg tattttctac aaagctacct ttaatacctg
     2761 tgtacaaatg aaataatccg ggaaccaatc caaagcgacc ttcaatgttt gcagaacctg
     2821 taacaggacg ttttgtacta tctgccgcta aaatatatag attgccacca atctttaaat
     2881 cccatgtttt accaatcaat ttatagtatg gcatgataga tattaagcat ccggcttccg
     2941 atttgtaagt atttgtgctt tcttctgtaa ggccttttat atttgtgtct atatcaatgt
     3001 cggctcctaa ttcgccattg ccaacctcgc ctcctaaatc agctttaaca agtatgttat
     3061 gattactaag tccaaaatta cgtcctagat aattataacc taatgttgtg ttatactgaa
     3121 atgctttttt cgtatttgta gaaaatagat taaagttaac tccaactgta gtataagttc
     3181 ctattgtatc tttaatagga tcgttaatta cgggtacagt agatttgccg tagtaatcaa
     3241 acccattata tgaaaagtct agtgcagaat tcaatctgga atctctaaat tgagattcaa
     3301 atgttacgtt tgctgatgtg ttttgtgtca ttgcacgtgg aacacttcca tcttccaatc
     3361 ttattttgct ccaatttgat aaatgtttaa ggtttacatc taataaatat ttatcgccac
     3421 gtaaaattgg ataataaaat tcgccgagta cttgccaata aaatcctaag ccggctttaa
     3481 atatcccatt cttagtatcg gcagtttcag gattttcata ctgttcagct tttaatttat
     3541 cgggtgtagt acttacttct tctgcttttg accatgttga atatgttacg gatggttgct
     3601 caggcgtata ttcttctttc ggtggaaccg ggcttatttt agttacttcg atggtagttg
     3661 gatcgtatgc cttttcaacc gtaacattac gtgttattga ttctgtttcg cttactgctt
     3721 tttgcccata agcaggtatt gatgctatta ggcataaaca agtgattaat ttaagggctt
     3781 tcatattaat tgttagatac ggtttcattt tcgtgttttt caattatttc taaacgttcg
     3841 gcaattgctt tcttgatatc ttcatctgct tctttataat tatcttgtaa acttagcaag
     3901 tactgtttcg cttcaaaata attttcttgt tcaatataga tgtcagaaag aagtataaat
     3961 gatttagcca accaataggt gtgtggtgtt cctttgtcga tgaaatcgaa tatttctttt
     4021 tcagcctcat cattttgact gttaatctta taagt
//
LOCUS       DAOPID010000141         3952 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_31136_length_3952_cov_3.670003, whole genome shotgun sequence.
ACCESSION   DAOPID010000141 DAOPID010000000
VERSION     DAOPID010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31136_length_3952_cov_3.670003"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1135)
                     /locus_tag="PK641_05255"
                     /pseudo
     CDS             complement(1..1135)
                     /locus_tag="PK641_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991856.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            complement(1218..2810)
                     /locus_tag="PK641_05260"
     CDS             complement(1218..2810)
                     /locus_tag="PK641_05260"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HPG55789.1"
                     /translation="MNIKSCLLIGVAMLTFSLPMSAAYELVWSDEFDCDELNPTVWTY
                     EIGAGGWGNGESQYYTSRADNLYLQDGKMIIVAKKEDYSGSSYTSARIKSNGKVQARY
                     GKVESLIKTPKANTGVWPAFWMLGKGADSWPYQGEIDIMEEMCTSNTTTWNRALSTYH
                     WNNNGMDGSYSNVNYGQSITFDEEVGDLWRIFGVEWTPSVLIGYVCDADGSNRQNVVT
                     MSTAAATDRSNGIYAFNYEFYMLYNIALGGAYVNYNIAQDFTSCQMQVDWVRIYQDKT
                     DYPQSTLTDNSSSCGTEIDVCTNSFASYTSMSNVCNENNVWNVVEGLASYSGTTITAN
                     PKLSYDNMYVVTVGNKNPLKASTEYTLSGTITSTENATVNIYVESNNDNKLQMFNDNE
                     ITLTAGQEYKFSISETAAERLDAPALVLSVSGGPADAVYTISNVSLKGSECVTAVNDV
                     VADNHYLVVYPNPISDYAVVESDEVIKSAEILSLNGKVLKQFNFNSDNYEINMSGISS
                     GLYILKAVYTNDNVEITKILKK"
     gene            complement(2852..>3952)
                     /locus_tag="PK641_05265"
     CDS             complement(2852..>3952)
                     /locus_tag="PK641_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HPG55790.1"
                     /translation="VLVKNENDFLPLSADNIKRVAVIGPMADAAHDQMGTWCFDGDKT
                     HSVTPLAAIKQQYGDKFEILYEKALISTRDYDKKDFAKAVSMASKSDVVLLFVGEEAI
                     LSGEAKCRADINLPGAQTELLKALEATGKPLVTIVMAGRPLTIEAETEASKSLFVAFH
                     GGTMAGPALIDLIFGKSVPSGKLAVTFPKMVGQIPIYYSDYKTGRPAQQPLHMIDDIP
                     LEAKQTSLGFCTYWLDAGLAPLFPFGYGLSYTKFEYFDLQLSSDKINMKDTLTVSCKI
                     KNTGKYDGTEVVQLYVRDYVGSLVRPLKELKGFQRIYIKKGEEKTVSFKISADQLSFW
                     NAKMQKVTEPGKFCVWVAPNSVEGLSADFVLN"
BASE COUNT         1206 a          820 c          609 g         1317 t
ORIGIN      
        1 agcctgtaca tgaacattcg ccggcagcat acaaatgctt aagcgaggtc cttgcttttt
       61 tatctactac aataccacct cacaaataat gagcagcggg aactacaggt atataatctt
      121 ttgtgatatc tactccaatg ctcaagcact tatcataaat catggggaaa tgttccttgg
      181 tttctgcagc agttttgtga gttacatcaa gataaacata atcacttccg gataatttca
      241 tctcagtatc aatagaacga gctactatat cacgcggcgc caaagatcca cgtttatcat
      301 atttgtacat aaattcttcg cctttaagat tacgcaatac tgcaccatat ccgcgcaaag
      361 cttctgtaat caaaaaggaa ggacgttcat taggattata taaagatgtt ggatggaact
      421 gtacgaactc catatctttt accaatcctt ttgctctata aaccattgag attccatctc
      481 cggtagcaat agaaggattt gtggtagttt gatatatctg tcctatacca cctgttgcca
      541 tcatagtaac ttttgacaag aaagtatgta cgtgtcttgt tttaggattt aaaacatata
      601 ctccgtaaca ttcaataccg ggagtagtat gcttaactaa ttgcccaaga tgatgttgcg
      661 ttattaaatc tatggcgaaa tgattttcaa atacctcaat attcggatgc tgattaattc
      721 ttttaaccaa agaatgctga atttcagcac cggtactgtc tttatgatgc agaattctaa
      781 attcagaatg gcccccttct ttatgaaggt cgaaattacc tgatgcatct ttgtcaaaat
      841 ctacacccca ctgaactaat tgatctattt cgcgtggtgc attttcaacc accatacgta
      901 caacagattc gtcacaaatt ccatctcccg caatcaatgt atcggcaata tgtttgtcaa
      961 atttgtcggt atctttagtt acagaagcaa ttcctccttg tgccaggttt gtatttgcct
     1021 cctccaattt ggttttgcat agaattgcaa ccttgccttt atcagcaacc ttaagggcaa
     1081 aactcattcc tgcaatgcca cttccaataa ccataaaatc aaacttgcgt accataattc
     1141 aaatattttc aatacacaaa agtacaaaaa aggaggatac tattgtaata tcctcctttt
     1201 aaagcttcga attcgaatta tttcttcaga atctttgtta tttcaacatt atcatttgta
     1261 taaactgcct ttaaaatata taaacccgaa ctaattcctg acatattaat ttcgtaatta
     1321 tcactattga aattgaactg tttaagaact tttccgttta gtgataagat ttctgcagac
     1381 ttaattactt cgtcagattc aactactgca taatctgaaa taggattagg atatacaaca
     1441 agataatgat tatctgcaac gacatcattt acagcagtta cacattcaga acccttcaaa
     1501 gaaacatttg atatggtgta aacagcatca gcaggtccgc cactaacaga taatacaagc
     1561 gccggagcat ctaatctttc agcagctgtt tcagatatgc taaacttata ttcttggcct
     1621 gctgtgagtg ttatttcgtt atcgttaaac atctgaagct tattatcatt gtttgattct
     1681 acatatatgt ttacagttgc attttcagtt gatgttatag ttccgctaag tgtatattct
     1741 gtacttgcct tcaaagggtt cttatttcca accgtaacaa catacatatt gtcatatgat
     1801 agtttcggat ttgcagtaat tgtagttcct gaatagcttg caagaccttc aaccacattc
     1861 cagacattat tttcattaca aacattcgac atagaagtat atgatgcaaa agaatttgta
     1921 catacatcta tttcagttcc acaacttgaa gagttatctg ttaatgttga ttgaggatag
     1981 tctgttttat cttggtaaat gcgaacccaa tcaacttgca tttggcatga agtaaaatcc
     2041 tgagcaatat tataattcac ataagcacct cctaatgcta tattatatag catgtagaat
     2101 tcgtaattaa aagcataaat accatttgaa cgatctgttg cagctgcagt actcatagta
     2161 actacatttt gcctatttga gccatctgca tcacatacgt aaccaattag aactgatggt
     2221 gtccactcta caccaaatat acgccatagg tctcctactt cttcatcgaa tgttatggac
     2281 tgaccatagt ttacattaga ataagaacca tccattccgt tattgttcca gtgatatgta
     2341 ctcaatgctc tattccatgt tgtagtattt gaggtacaca tttcttccat aatgtcaatt
     2401 tcaccttggt acggccaaga atcagctcct ttacctaaca tccaaaatgc gggccaaaca
     2461 ccggtattag cttttggggt tttgattaat gattctacct taccatatct tgcctgtact
     2521 ttaccgttac tttttatacg tgcagaagta tagctacttc ctgaatagtc ttcttttttg
     2581 gcaactataa tcattttacc gtcttgaaga tataaattat cagctctact tgtgtaatac
     2641 tgagattcac cattacccca tccaccggca cctatttcgt aggtccatac tgttgggttt
     2701 aattcatcac agtcgaactc gtcagaccac actaattcgt aagcagcaga cataggcaga
     2761 gagaatgtca gcatagcaac tccgattaaa agacagcttt taatattcat aatatttaat
     2821 ttacggagtt atctattcga caaactcccg gttagttaag tacgaaatcg gccgacagac
     2881 cttctacact gtttggagcc acccaaacgc aaaatttacc cggttctgta actttttgca
     2941 tttttgcatt ccaaaaagac aattgatctg ccgaaatctt aaatgaaact gttttttctt
     3001 ctcccttttt aatataaatc ctttgaaatc cttttaattc ttttaaagga cgaactaatg
     3061 agcctacata gtctctaacg tacaattgca ccacttcggt accatcgtat ttgcctgtat
     3121 ttttaatctt acatgatacc gttagggtat ctttcatgtt tattttatct gacgataatt
     3181 gtaaatcaaa atactcaaat tttgtatatg ataaaccata accgaatggg aataatggag
     3241 ctaaacctgc atctaaccaa tatgtacaga aacctaaaga tgtttgcttt gcttctaacg
     3301 gaatatcatc tatcatatga agaggttgtt gtgcaggacg ccctgtttta taatcgctat
     3361 aataaatagg aatctgacca accatttttg ggaatgtaac agctaattta cccgatggaa
     3421 cagatttacc aaaaatcaaa tctatcaaag caggccctgc cattgtaccg ccatgaaatg
     3481 caacaaataa tgattttgag gcttctgtct ctgcttctat cgttaacggt cttcctgcca
     3541 tcactattgt tactaaaggc tttcctgtgg cttctaatgc ctttagcaat tcggtttgag
     3601 ctccaggtag attaatatcg gctctacatt ttgcttctcc cgacaatatt gcttcttcac
     3661 ctacaaataa caatactaca tcagactttg atgccataga tacagccttt gcaaaatcct
     3721 ttttatcgta atcgcgagta cttattaaag ctttctcgta taaaatttca aacttatcac
     3781 catactgttg ttttattgca gcaagaggag ttacagaatg tgttttatct ccatcaaagc
     3841 accatgttcc catttggtcg tgagcagcat cggccattgg tccaattaca gccacccttt
     3901 taatattatc tgcagacaat ggtaagaaat cattttcatt ttttactaaa ac
//
LOCUS       DAOPID010000142         3912 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_31598_length_3912_cov_4.558984, whole genome shotgun sequence.
ACCESSION   DAOPID010000142 DAOPID010000000
VERSION     DAOPID010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31598_length_3912_cov_4.558984"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="PK641_05270"
     CDS             <1..915
                     /locus_tag="PK641_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55791.1"
                     /translation="KYIAQDWDGFQKLVQESSVKDKDLILRVLGAYQDAEQREKEIKN
                     LSAAYTELASDILPKLRRSHLALTINVAGKTDAELLEIAKSNSDSLTVEELLYAAKLA
                     CTSGNKEDAAKFTAANAAQNPSDWRTQNNQGAVNFAKGDLSATKSALNNSVNNGGANE
                     PETNFNLGLVALEENQPNQAQQYFGKAAGVEQLGEGQGLMYIQQGEYAKAVSAFGNAS
                     TNNAALAQMLVNNNDKAISILNNVSEKDATTYYLLAVCSARTANDAQVYSNLSQAISL
                     DANYSARAKSDLEFGKYWTLDSFKNVVK"
     gene            complement(946..1854)
                     /locus_tag="PK641_05275"
     CDS             complement(946..1854)
                     /locus_tag="PK641_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55792.1"
                     /translation="MIQKLIRFIKDWTLLLALIFGFIFNHFFGYVASTYTEIIPTLIF
                     CMLLLSFSKLEGGTIKSPFLHIILLGLQLIFGISSYYIFKHFNVDLAEGIMIIILVST
                     ATSAPVVVNLMKGKIEFITQYLLLSSISASIFLPIWFSWINHGFENSFWSSFTRIATY
                     TLPTILFPLVIALCTRKFAPKTHTYIRKCTSATYYMWAFALMILIGSAVNSFLTQDNP
                     NYLFAIIAFIAAFIICSAQLIIGKLIGRKYDESIGAGQAIAQKNTVLAIWLSRSFLSP
                     MASLVPTMYIIAQNIINSLQIKKATK"
     gene            complement(1921..2103)
                     /locus_tag="PK641_05280"
     CDS             complement(1921..2103)
                     /locus_tag="PK641_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55793.1"
                     /translation="MKNLGILVVILGALALIIPSFANFESNTTLAIGLALLIIGIVIH
                     IIVSRKSLDSDNKTKK"
     gene            complement(2189..>3912)
                     /locus_tag="PK641_05285"
     CDS             complement(2189..>3912)
                     /locus_tag="PK641_05285"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007762338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HPG55794.1"
                     /translation="DNSIDEFRMKFGNHIDISINGQEVSVRDYGRGIPQGKLVDCVSI
                     INTGGKFDSQNFQKSIGLNGVGTKAVNALSSHFVATSFRNGMVKRAEFTNGRLSQESD
                     LEPTTEKDGTLITFSPDSSEKLFGNYQFKNEFIETMLRNYSYLNPGLTLSFNGTRFSS
                     KNGLLDLLNENITAETIYPIVHLQGEDIEIAFTHTEQYGEEYYSFVNGQHTTQGGTHQ
                     SAFREAFARTIKEFYNKNIDYADIRNGFSAAISIAVQEPVFESQTKIKLGSKDMGPDG
                     PSVNKFISDFVKKEVDNYLHKNPEVADILLRKILESEKERKAIAGVSKQAKERAKKAN
                     LHNRKLRDCRFHLNEGKDEEKKLASCIFITEGDSASGSITKSRDVNTQAVFSLRGKPL
                     NSFGLTKKVVYENEEFNLLQAALNIEDGLDGLRYNKVIIATDADDDGMHIRLLILNFF
                     LQFFPDLVKSGHVYILQTPLFRVRDKKETFYCYTDEERINAINKLGKKAEITRFKGLG
                     EISPDEFVHFIGKDIRLDQVSLRKEDALATLLSFYMGKNTPERQDFIIQNLRIEEDLE
                     EDLLSTE"
BASE COUNT         1268 a          662 c          668 g         1314 t
ORIGIN      
        1 aaatatattg ctcaagattg ggatggattc caaaaattgg ttcaagaatc aagcgtgaaa
       61 gataaagatc ttattttacg tgttttaggt gcataccaag atgctgaaca acgtgaaaaa
      121 gaaatcaaga acttatctgc tgcttacaca gaattagctt ctgatattct tcctaaatta
      181 cgtcgttctc atttagcttt aactataaat gtagctggta aaacagatgc agaattgtta
      241 gaaatagcta aatcaaattc agattctttg actgtagaag agttgctgta tgcagcaaaa
      301 ttagcttgca cttcaggaaa taaggaagat gctgctaaat ttacagctgc aaatgctgct
      361 caaaatcctt cagattggag aactcaaaac aaccaaggtg ctgttaattt tgcaaaaggt
      421 gacttaagtg ctacaaaatc agccttgaat aactcagtta ataatggtgg tgctaacgaa
      481 cctgaaacca acttcaactt aggcttagtt gctttggaag aaaatcaacc aaaccaagct
      541 caacaatatt ttggtaaagc tgcaggtgtt gaacaattag gtgaaggaca aggactaatg
      601 tacatccaac aaggtgaata tgctaaggct gttagcgcat ttggcaatgc aagcacaaac
      661 aatgctgctt tagctcaaat gttggtaaac aacaacgaca aagcaatctc tatattgaat
      721 aatgttagcg aaaaagatgc tacaacatat tatttattgg cagtatgcag tgctcgtaca
      781 gcaaacgacg ctcaagttta ttcaaattta tcacaagcaa tcagtttaga tgctaattac
      841 tcagctcgcg caaaatctga tcttgagttc ggtaaatact ggacattaga ttcattcaaa
      901 aatgtagtga aataagtttt atttctatat agtaaaaaag gtcacctatt tggtggcctt
      961 ttttatttgt aatgaattaa taatgttctg tgcaataata tacatagttg gaacaagtga
     1021 tgccataggt gataaaaatg agcgagaaag ccatattgcc agtactgtat ttttctgagc
     1081 tattgcttgt cctgcgccaa tactttcgtc atatttgcgt cctataagtt tacctataat
     1141 tagttgcgca gagcagataa taaatgcagc tataaaggca attattgcga atagataatt
     1201 aggattatct tgcgttaaaa atgagtttac agcgctgcca ataagtatca taagtgcaaa
     1261 agcccacata taatatgtag ccgatgtgca tttccgtatg taagtatgtg tcttaggtgc
     1321 gaatttacgc gtgcataatg ctataactaa agggaagagt atcgttggca aggtatatgt
     1381 tgcaatgcgc gtaaaagagc tccaaaatga gttttcaaaa ccatgattaa tccacgaaaa
     1441 ccaaatcggt aaaaatattg atgcagaaat gctgcttaat agtaaatact gtgtaataaa
     1501 ttcaattttg cccttcatca gatttactac aacgggtgct gaagtagcag tagataccag
     1561 aataataatc attattcctt ctgccaagtc tacattaaag tgtttgaaaa tataatatga
     1621 tgaaatgcca aagattaatt gtagtcctaa taaaattata tgcagaaatg gagacttaat
     1681 agtaccacct tctaatttag aaaaagaaag taataacata caaaaaataa gagtaggaat
     1741 tatttccgtg tatgtagaag caacgtatcc gaagaagtga ttgaaaataa aaccaaagat
     1801 tagtgctaat aatagagtcc aatcttttat gaaacgtatg agtttttgta tcatggctga
     1861 ttaatacaaa agcctccttt aacaaaggag gcttgaatta tttattatgt aattgtcaag
     1921 ttattttttt gttttattgt ctgaatctaa actttttctg ctaacaatta tatgtattac
     1981 aatgcctata attagtaatg ctaatccgat agctaatgtt gtgtttgatt caaaattagc
     2041 aaatgatgga ataataagtg ccaatgcacc taaaattact actaaaatac ctaaattttt
     2101 catattgttt acttttttgt ttatgtcgaa tttgtgttac aaaggaaata ataattttct
     2161 tacttaaaaa ataaaacctt tgtttttttt actcagtgct taataaatct tcttctaaat
     2221 cttcttcaat acgtagattt tgaattataa aatcttgtct ttcaggcgta tttttaccca
     2281 tgtagaagct aagcagtgtg gcaagagcat cttccttacg taaacttact tgatccaatc
     2341 ttatgtcttt gcctataaaa tgaacaaact catcaggtga gatttcacct aaacctttaa
     2401 atcttgttat ttctgccttc ttaccaagct tgttaattgc atttatacgt tcttcgtctg
     2461 tataacagta aaacgtttct tttttgtcgc gaacacgaaa tagtggagtt tgtagtatat
     2521 atacatgtcc gctttttact aagtcaggga aaaattgaag aaagaaatta agaattagta
     2581 atcttatgtg catgccatca tcatcggcat ctgttgcgat tataacctta ttatatctca
     2641 atccgtctaa accgtcttca atattcagtg cagcttgtaa taagttaaat tcttcgtttt
     2701 cgtaaactac tttctttgtt agtccgaaac tattaagcgg tttccctctt aaactgaata
     2761 ctgcttgtgt attaacatcg cggctctttg ttatagaacc acttgcagag tcaccctcag
     2821 taataaagat gcaactcgcc aatttctttt cttcatcctt accttcgtta aggtggaaac
     2881 gacaatctct taacttgcga ttgtgtaaat tagccttctt ggctctttct ttcgcttgtt
     2941 ttgaaacgcc tgcaatagct tttcgctctt tctccgattc taaaatcttg cgtaataaga
     3001 tgtctgcaac ttccggattc ttatgtaggt aattatcgac ctcttttttt acaaaatctg
     3061 aaataaattt attaacggaa ggtccatcgg gtcccatgtc tttagaccca agtttaatct
     3121 ttgtttgaga ctcgaataca ggttcttgaa ctgctataga aattgctgca gaaaaaccat
     3181 ttcgaatatc ggcataatct atattcttat tgtaaaattc ttttatagtt ctagcaaagg
     3241 cttcgcgaaa tgctgattga tgagtacctc cttgtgttgt atgctgacca tttacaaaag
     3301 agtagtattc ttctccgtat tgttctgtgt gtgtaaacgc tatttctata tcctcgcctt
     3361 gtaagtgaac tattggatat atggtttcgg cagttatgtt ttcgtttaga aggtcaagta
     3421 atccattctt tgacgaaaac cttgtgccat taaagctaag agtaagtccc ggattcaaat
     3481 agctgtagtt acgcagcatt gtttcgatga attcgttctt aaattgataa ttaccgaata
     3541 atttttcaga gctgtcaggc gaaaaagtta taagagttcc atctttttcg gttgtaggtt
     3601 ctaaatcaga ttcttgagat aatctgccat tagtgaattc cgcacgcttt accataccat
     3661 ttctaaatga cgtagcaacg aaatgcgaag acaaagcatt tacagctttt gtgccaactc
     3721 catttaatcc aatagacttt tggaagtttt gagagtcgaa ttttccacct gtgttgatta
     3781 ttgatacaca atctactaat ttgccttgtg ggatacctct tccatagtct ctgacagaaa
     3841 cctcttgacc attaatacta atgtcaatat ggtttccgaa tttcatacga aattcgtcaa
     3901 tagaattatc ta
//
LOCUS       DAOPID010000143         3909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_31643_length_3909_cov_4.092372, whole genome shotgun sequence.
ACCESSION   DAOPID010000143 DAOPID010000000
VERSION     DAOPID010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31643_length_3909_cov_4.092372"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PK641_05290"
     CDS             <1..271
                     /locus_tag="PK641_05290"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009433947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HPG55795.1"
                     /translation="EGEIINELQKLGFTYDGIILNAGAYTHTSVAIRDCIKSITTPVI
                     EVHLSNVTARESFRHESLITPVCKGCILGFGLHSYDLAIQSFLID"
     gene            343..2778
                     /locus_tag="PK641_05295"
     CDS             343..2778
                     /locus_tag="PK641_05295"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HPG55796.1"
                     /translation="MKKIFTCAILFMCALSTFAQSGKIYGTIIDNSTKESMDYVNIAL
                     YKGSSNTPEIGGFSDESGQFNIQVPYGTYTLKASFLGYNSFEKKVIVTKDKPNVKIGN
                     IILYEDAKMMKEIEVVGQKSGMSLEIDKKVFNVDQSIVSEGASASEILQNIPSVDIDT
                     DGNVALRNSSSVEIWINGKPSGLSDTDKGQVLEMLPAETVEKVELLTNPSAKYNPEGS
                     VGIINIVLKENRKGGYFGNVSTGVNYQEGSAYPGGNLGLNFTYSDNKWDVNFNASVRS
                     RKRDRGSYNYRMSFEDADTTYLNQTNTSTQDRVNGFLKAGATYHIDKKNEIGLSAYGM
                     YSQNWNKGNIEYATLNKDKDTTHLRERNNESTGNMGFYNVAIDYRHQFEKDTHEISAN
                     LNYFGRQRNSNSSYLTKESGDINPLRTYELQTLNESNNSASVQVDYFNKFTKNSKFEA
                     GLKGSLNFTESIDKTFDSIYIDNTLIEDITKYNPFYYSEQIYAAYATYGNKFDWFSFQ
                     LGIRAEETITKANEVQRSYFQAFPSVYLSFELPKDNEIQVNYTRRINRPRGRRINNYI
                     DRSDPTNISFGNPLLMPEFANSVEINYLKNWERHTLSVGLFYRYTENVIQSVKRLTDL
                     GIMENTYENITYSQNAGAEIVAKNRLFNNYLDLTTSLSAYYYQLGANEEWNVAETNSF
                     SWNARINANVKIISNLSAQITAYYNSPHIVAQGTVGDNYAMDLGLRANFLNKTLSLSL
                     SVRDVLDSRTHTQNITWGENFYQENANTTCGRSYRLTLTYNFGNMKAKKQKKNENTNE
                     DSYEDTDNGMGSF"
     gene            2845..3669
                     /locus_tag="PK641_05300"
     CDS             2845..3669
                     /locus_tag="PK641_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55797.1"
                     /translation="MKKLILTIASIAVTSILFAQIQTNASNQYYNNQNEYYNAQLPID
                     IKSVSGPNKEVVNVFYITFNYPSQSIENAIVERLKKEGLEGKKMKNNFFAFKGVKYNY
                     LWNRTFDFYISVIGSQNAGTVNLLMSQGYDNFIDTNDYEIQKRVSDWLTGLDIDIQRY
                     IHSQTLNGEIKEYEEAQKELKKLNKEKSKIESKIAKKDIERRTFEAKRTIPDQNNMNS
                     IDPNILEKEQKKANDLEEDRNKLDYELKEIKQKIEAAEKHVAEHDKAIKEIKSAKP"
     gene            3682..>3909
                     /locus_tag="PK641_05305"
     CDS             3682..>3909
                     /locus_tag="PK641_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55798.1"
                     /translation="MQQLNVLFSKTFKEDATSIQKIAESGSNRRYQRMYGSSFSVIGV
                     YGESIRENKAFIAMSQHFKQCNINVTLFKMLR"
BASE COUNT         1504 a          615 c          678 g         1112 t
ORIGIN      
        1 agagggtgaa atcataaacg agttacaaaa gttaggattt acttatgatg gcatcatcct
       61 aaatgccgga gcatatacac acacatctgt agcaatacgc gattgtatca aatcaatcac
      121 aactcctgtt attgaagttc atttatcaaa cgttactgca agagagagtt ttagacacga
      181 gtcattaatt acacctgtat gtaagggctg tattcttggt tttggtctac actcatacga
      241 tttagcaata caaagtttcc ttatcgacta gtaaggctat taaataagcc tgattgtatc
      301 gaaacatttt aaacttattt tattttaggg tgtctaagcg acatgaaaaa aatatttact
      361 tgcgccattt tatttatgtg tgctttatca acgtttgccc aaagtggtaa aatatatggt
      421 actattatcg ataatagtac taaagagtca atggattatg taaatatcgc attgtacaag
      481 ggcagttcaa atacacctga aataggagga ttttctgatg aatcgggaca atttaatatt
      541 caagtccctt atggcactta tacactaaaa gcatctttcc tcggttacaa tagttttgaa
      601 aaaaaagtaa ttgtaacgaa agacaaacca aatgttaaaa ttggcaatat tattctttac
      661 gaagatgcaa aaatgatgaa agagatagaa gtcgttggtc aaaaatcagg catgagccta
      721 gaaatagaca aaaaagtttt caacgttgac caaagcattg tatccgaagg agcatctgca
      781 tccgaaattc tacaaaatat accttctgtt gacattgata cagatggtaa tgttgcttta
      841 cgcaatagtt cctctgtcga gatttggatt aatggaaaac catcaggatt gtcagataca
      901 gacaaaggcc aggttctgga aatgctaccg gctgaaactg ttgaaaaagt tgaattactt
      961 actaatccat cagctaaata caaccccgaa ggcagtgtgg gtattattaa tattgtatta
     1021 aaagaaaatc gtaaaggcgg ttatttcggg aatgtttcaa ccggtgtaaa ttaccaagaa
     1081 ggctccgcat accccggagg aaatttagga cttaatttta catatagcga taataaatgg
     1141 gacgtgaatt ttaacgcaag tgtacgttca agaaaaagag atagaggtag ttataattat
     1201 cgaatgagtt ttgaagacgc tgatacaaca tatttaaatc aaacaaacac atctacgcaa
     1261 gacagagtta atgggttttt aaaggcaggg gcaacatatc acattgacaa aaaaaacgaa
     1321 atcggattat ctgcttacgg catgtatagt caaaattgga ataaaggaaa cattgaatat
     1381 gccacactca acaaagacaa agacacaaca cacttacgcg aaagaaacaa tgagagcaca
     1441 ggtaatatgg gattctacaa tgtagctata gactatcggc atcaatttga gaaagacaca
     1501 cacgaaatat ctgctaattt aaactatttt ggacgtcaac gtaatagtaa tagttcttac
     1561 ttgacaaaag aatccggaga tattaatcca ttacgtactt acgaacttca gactcttaat
     1621 gaaagtaata attcagcatc tgtacaagtt gactacttca ataaatttac caaaaactct
     1681 aaatttgaag ccgggctgaa aggaagttta aattttactg aaagcattga caaaacattt
     1741 gattcaatat atatagataa tacactgatt gaagatatta caaaatacaa tccattctat
     1801 tattcagaac agatttatgc tgcatacgct acatacggca ataaatttga ttggttctca
     1861 tttcagttgg gaatacgtgc agaagaaact attaccaagg caaacgaagt acaacgcagt
     1921 tatttccaag catttccgtc agtatattta agttttgagt taccaaagga taatgaaata
     1981 caagtaaact acacaagacg tattaataga ccacgtggca gacgaataaa taattatatt
     2041 gatagatctg atcctaccaa tattagcttt ggtaatccat tattaatgcc tgaatttgct
     2101 aattcagtcg agattaatta tctaaaaaat tgggaaaggc atacattatc tgtcggtttg
     2161 ttttatcgtt atacagaaaa cgttatacaa tctgtaaaac gactgacaga tcttggtatt
     2221 atggaaaaca cttacgagaa tattacgtat tcgcaaaacg caggagctga aatcgttgca
     2281 aagaatcgtc ttttcaataa ctatcttgat ttaacaactt cgcttagtgc ttattactat
     2341 cagttaggag ctaacgaaga atggaatgta gctgaaacaa atagtttttc gtggaacgct
     2401 cgtatcaacg ccaatgttaa aataataagt aacttgtctg cacaaataac agcatattac
     2461 aactcaccac acattgtagc tcaaggtaca gtcggcgata actacgcaat ggatttagga
     2521 ttaagagcaa atttcttgaa taaaaccctg agcttaagtt tatctgttag agatgtatta
     2581 gattctcgta cgcacacgca aaacattact tggggagaaa atttttatca agaaaatgca
     2641 aacacaacat gtggacgtag ttatagatta acattgacat ataattttgg caatatgaaa
     2701 gccaaaaaac aaaagaagaa cgaaaataca aatgaagatt catatgaaga tacggataac
     2761 ggcatgggca gtttctaaaa aaactgtata tttgtaaatc ttttatgaga cagatgttat
     2821 cagtaataat gaattttata caccatgaaa aaactaattt taaccattgc tagcattgct
     2881 gtaacaagta tattgtttgc tcaaatacaa acaaatgctt caaatcagta ttacaacaat
     2941 caaaatgagt attataatgc acaactgcct attgacatta aaagtgttag tggcccaaac
     3001 aaagaagtag taaatgtttt ttatatcaca ttcaactacc cttcccaatc aatagagaat
     3061 gcaattgtag aacgacttaa aaaagaaggt ctagaaggta agaaaatgaa aaataatttc
     3121 tttgcattca aaggtgttaa gtacaattac ctatggaaca gaacttttga cttctacata
     3181 agtgttatcg gatctcaaaa cgcaggaacc gttaatttgc ttatgtctca aggatatgat
     3241 aactttattg acacaaacga ttatgaaatc cagaaacgag tatctgattg gttaaccggt
     3301 ttggatattg atattcaaag atatattcat tcacaaactc ttaatggaga aattaaagaa
     3361 tatgaagaag ctcaaaaaga gctcaaaaaa ctaaacaaag aaaaatcaaa aatagaatct
     3421 aaaattgcaa aaaaagatat tgagagacgt acttttgaag ccaaacgcac aattcccgat
     3481 caaaataaca tgaacagtat agacccgaac atacttgaaa aagaacagaa gaaagcaaat
     3541 gatcttgaag aggataggaa taaacttgat tacgaattaa aagagattaa acaaaaaatt
     3601 gaagctgcag aaaaacatgt tgcagagcac gataaagcaa ttaaagagat taaatctgct
     3661 aaaccataat taaggcttat catgcaacaa ttgaatgtct tattttcaaa aacatttaaa
     3721 gaagacgcta cttcaataca aaagattgca gaatcaggat caaacagacg ctatcagcgc
     3781 atgtatggta gttcatttag cgtaattggt gtgtatggcg aatctattcg cgagaataag
     3841 gcatttattg caatgtcgca acattttaaa caatgtaaca ttaatgttac attgtttaaa
     3901 atgttgcga
//
LOCUS       DAOPID010000144         3907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_31663_length_3907_cov_5.239616, whole genome shotgun sequence.
ACCESSION   DAOPID010000144 DAOPID010000000
VERSION     DAOPID010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31663_length_3907_cov_5.239616"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="PK641_05310"
     CDS             <1..1137
                     /locus_tag="PK641_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HPG55799.1"
                     /translation="ILWGPPGVGKTTLARIMAEKSERGFYSLSAVNSSVKDVREVIER
                     ADKSRFFQSPPPILFIDEIHRFSKSQQDSLLAAVEKGTVVLIGATTENPSFEVITPLL
                     SRCQVYVLKSLDKTALLHLAEVALKKDIELKKRHVELKETEALLQYSGGDARKLLNAL
                     ELVVGTESDRDVIITNDLVTEKLQENPAAYDKQGEMHYDIISAFIKSIRGSDPDAAVY
                     WLARMIAGGEDPKFIARRLLISASEDIGLANPNALLLANACFDAVNKIGFPEARIVLS
                     ETAIYLASSPKSNSAYLAIDSALEEVNKSTNLSVPLQLRNVPTNLMRDMQYGKGYKYP
                     HDFEENFVNQEYMPKELKNHKFWHPQSNTSESRLSEFLKKMWGK"
     gene            1354..>3907
                     /gene="carB"
                     /locus_tag="PK641_05315"
     CDS             1354..>3907
                     /gene="carB"
                     /locus_tag="PK641_05315"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HPG55800.1"
                     /translation="MKQSFKKVLLLGSGALKIGQAGEFDYSGSQALKAMREEGIETVL
                     INPNIATIQTSEGVADKVYFLPVNPYFVERIIEKERPDGILLAFGGQTALNCGTALYQ
                     NGILKKYGVNVLGTSVEAIMITEDRDLFVKSLKQIDAKTPISQAVESMEDALTVARRI
                     GYPVMVRSAYALGGLGSGVCANETEFIQLCESSLAYSKQILVEESLKGWKEIEFEVIR
                     DANDHCFTVVPMENVDPLGIHTGESIVVAPIISLTKEQISLLEKIAAQVVRHIGIVGE
                     CNIQYAFNAETNDYRIIEINARLSRSSALASKASGYPLAFVAAKLALGYTLDQIGEMG
                     TLNSAYVAPSVDYIIAKIPRWDLTKFVGVSREIGSSMKSVGEIMSIGKSFEEIMQKGL
                     RMIGQGMHGFVGNTDLTFDDVEKALSHPTDLRIFAIADAFEKGYTVAQIEKLTKIDVW
                     FLEKLENIYKYTKVLSKFSKIEDLTAEVLKEAKRLGFSDFQIARYVENSKGNMESEML
                     RVRKHRKSLEINPKVCRINTVASENPDKTNYLYMTYGAEKSFKPVKSNKESVVVLGSG
                     AYRIGSSVEFDWCSVNAVQTARKLGYQSVMINYNPETVSTDYDMCDRLYFDELSFERV
                     LDVLDIENPKGVIVSVGGQIPNNLAMKLYRQNVPILGTSALSIDRAEDRHKFSAMLDT
                     LGIDQPRWRELSSFEDIDEFISEVGFPVLIRPSYVLSGAAMNVCYDKDQVRSFLTMAA
                     KVSKEHPVVVSSFLQNAKEIEFDAVAKQGEIVEYAISEHVEFAGVHSGDATLVFPAQK
                     IYFETMRRIKKVSKMIAKELQITGPFNIQYLAKNNDVKVIECNLRASRSFPFV"
BASE COUNT         1300 a          603 c          771 g         1233 t
ORIGIN      
        1 atattgtggg gtcctccggg tgtaggaaaa accacattgg caagaataat ggctgaaaaa
       61 tcagagcgtg gattttatag tcttagtgca gttaattcat ctgtaaaaga tgttcgtgaa
      121 gttattgaga gggcagataa atctcgtttc tttcaatctc ctcctccgat tctatttatt
      181 gacgagatac atcgttttag taaatctcaa caagattcct tacttgcagc agtagaaaaa
      241 ggtacagtcg ttttgatagg agcgactaca gagaatccat catttgaagt aattaccccg
      301 ttgttgtcca ggtgtcaggt ttatgttctt aaatctttgg ataaaacagc tttgttgcat
      361 cttgccgaag tcgcattaaa aaaagatata gaacttaaaa aacgtcatgt tgagcttaaa
      421 gaaacagagg ctttgttgca atattcggga ggtgacgcac gtaaattgct taacgcttta
      481 gaattagttg ttgggacaga aagcgataga gatgttatta ttacaaatga tttggtaact
      541 gaaaaattgc aagagaatcc ggcagcctac gataaacaag gcgaaatgca ttatgatatt
      601 atttctgcct ttattaaatc aatacgtgga agtgatcctg atgcagcagt gtattggttg
      661 gctcgaatga tagcaggagg tgaagatcct aaatttatag cacgaaggct acttatttct
      721 gcatccgaag atataggttt agctaatcca aatgctttgc tattagcaaa tgcttgtttt
      781 gatgcagtta ataaaattgg attccctgaa gcgcgtatcg ttttgtctga aaccgcaata
      841 tacttggcat caagtcctaa aagtaattct gcatatttag ccattgacag cgccctcgaa
      901 gaagttaata aatcgacgaa tttatcagtt ccattacaat tacgcaatgt tcctacaaat
      961 ttaatgcgcg acatgcaata tggtaaaggg tataaatatc cacatgattt tgaagaaaat
     1021 tttgtaaatc aggaatatat gcctaaggag ttgaaaaatc ataaattctg gcatccgcag
     1081 tcaaatactt cagaatcaag actaagtgag tttttgaaaa aaatgtgggg gaaataagca
     1141 aaatttattt tttcatttgt ctaaaaatat atacctttgc tgcgatttaa accaaactaa
     1201 atgaacaagc agttttatct cctgtacgat ggcaaattta aacatgccat tgatattttt
     1261 ttaggttttg tttttatctg atgcgtttgc tttttggcgg cgcttttttt ttgtaaaaaa
     1321 gtacaaacat acaatattat ataacatatc atcatgaaac aatcttttaa aaaagtattg
     1381 ctgcttggct caggagcctt gaaaattggt caagcaggtg agttcgatta ttccggttca
     1441 caagcgttaa aagctatgag agaagaagga attgaaacag ttctgattaa tccgaacatt
     1501 gcaacaattc aaacctctga aggtgtagct gataaagttt acttcttacc tgtaaatcca
     1561 tatttcgttg aaagaataat tgaaaaagaa cgtcctgatg gtatattgtt agcttttgga
     1621 ggtcagacag cattaaattg cggtacggct ttgtaccaaa acggaatact taaaaaatat
     1681 ggcgttaatg tattgggaac atctgttgag gccattatga ttacagaaga ccgcgattta
     1741 tttgttaaaa gcttaaaaca aatcgatgcg aaaactccga tatctcaagc tgtcgaaagc
     1801 atggaagatg cactaacagt tgcacgccgt ataggctatc ctgttatggt ccgttctgct
     1861 tatgctcttg gaggtttggg atcaggcgta tgtgctaacg aaacggagtt tatccaatta
     1921 tgcgaaagtt cattagcata ctcaaaacag attttggttg aagagagtct taaaggttgg
     1981 aaagaaattg aatttgaggt aatacgcgat gcaaacgatc attgctttac tgttgttcca
     2041 atggagaatg ttgatccact aggtatacat acaggtgaaa gtatagttgt tgcacctatt
     2101 atatctttaa ctaaagaaca aatttcactt ttagagaaaa tagctgctca agttgtacgt
     2161 catatcggaa ttgtaggtga atgtaacatt caatatgctt tcaatgccga aaccaatgat
     2221 tatcgtatca tcgaaattaa tgcacgttta agtcgttctt cagctttagc atctaaagca
     2281 tcaggttatc cgcttgcatt tgtagctgct aaattagcat taggatatac ccttgatcaa
     2341 ataggtgaga tgggtactct taattctgct tatgtagctc cttctgttga ttatataatc
     2401 gcaaaaatac cacgttggga ccttacaaaa tttgttggag taagtcgtga aataggttct
     2461 tcaatgaagt ctgtaggcga aattatgtca ataggtaaat ctttcgaaga aatcatgcaa
     2521 aaaggtctac gtatgatagg tcaaggaatg catggttttg taggaaatac tgatttgact
     2581 tttgatgatg tagaaaaagc attatctcat cctacagatt tacgtatatt cgcaattgct
     2641 gatgcattcg aaaaaggata cactgtagct caaattgaga aattgactaa aatagatgtt
     2701 tggttcttag aaaaattgga gaatatatat aaatacacta aagttttatc taaattttca
     2761 aaaattgaag atttgaccgc agaagtattg aaagaggcaa aacgcttagg attctctgat
     2821 ttccaaatag cacgttatgt tgagaattca aaaggaaata tggaatctga aatgttacgt
     2881 gtgcgtaaac acagaaaatc attagaaatt aatcctaaag tttgtcgtat aaataccgta
     2941 gcttctgaaa atcctgataa aacaaattat ttgtatatga cctacggcgc tgaaaaatct
     3001 ttcaagccgg ttaaatcaaa taaagaaagt gtagttgttt taggttcagg agcatatcgt
     3061 atcggatcat ccgtagaatt tgactggtgt agcgtaaatg cagtacaaac agcccgtaaa
     3121 ttaggctatc aatctgtaat gattaattac aatcccgaaa cagtatctac cgattatgat
     3181 atgtgtgatc gtttatattt cgacgagtta tcattcgaaa gagtacttga tgttttggat
     3241 atagaaaacc ctaagggagt tattgtttct gtaggaggtc aaattccaaa taatcttgca
     3301 atgaaactat atcgtcagaa tgtacctatt ttaggaacct ctgcattatc tattgacaga
     3361 gcagaagatc gtcataaatt ttcagcaatg ttggatactc tgggtataga tcaacctcgt
     3421 tggcgcgaac ttagcagttt tgaagatata gatgaattta tttctgaagt aggatttccg
     3481 gtattgattc gtccttcgta tgtattatca ggagcagcaa tgaacgtatg ttatgataaa
     3541 gatcaagttc gttcattttt aacaatggct gccaaggtgt ctaaagaaca tcctgtagta
     3601 gtatcatcat tcttgcaaaa tgcaaaagaa attgagtttg atgctgttgc aaaacaaggt
     3661 gaaatagttg aatatgcaat atcagaacat gttgaatttg ccggagttca ctccggtgat
     3721 gcaaccttag tattccctgc acaaaaaatc tattttgaaa caatgcgtcg aatcaagaaa
     3781 gtttcaaaaa tgattgcaaa agaattgcaa attacaggac ctttcaatat ccaatattta
     3841 gcaaaaaata atgatgtaaa agttattgaa tgtaatttac gtgcatcacg ttcgttccca
     3901 tttgtat
//
LOCUS       DAOPID010000145         3884 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_31948_length_3884_cov_4.759467, whole genome shotgun sequence.
ACCESSION   DAOPID010000145 DAOPID010000000
VERSION     DAOPID010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31948_length_3884_cov_4.759467"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="PK641_05320"
     CDS             complement(<1..886)
                     /locus_tag="PK641_05320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPG55801.1"
                     /translation="MNNSSLKIGLFNECFPPIQDGVAQVVENYARLLQERGNDVTVAT
                     AKVPFFDYSQPYHVRNFLSIPVLMRMPYRFGVPIWMAPFQPIMKYNADIFHGHSPFTA
                     GRIALKLSRKYNAPFIMTFHSKFRDDFRRSVPSDTIVDLMIKRIMSLYEYADEVWIPQ
                     PAVLDVMREYGYKGKVEVVPNAIDYDLGNQDINILKAESRCKLGLKDNEICLLFVGQI
                     IYEKNIQYILDSVKLLKDVPYKMYFVGKGYAAIEFHEKIKQYGLQDKIFMTGQLQDRS
                     ALQVYYAAADLFLFPSLYD"
     gene            1234..2754
                     /gene="guaA"
                     /locus_tag="PK641_05325"
     CDS             1234..2754
                     /gene="guaA"
                     /locus_tag="PK641_05325"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HPG55802.1"
                     /translation="MLEKIVILDFGSQTTQLIARRVRELNTYCEILPYNKYPQNDSTI
                     KGVILSGSPFSVYDKAAFKTDLRQFVGKLPVLGLCYGAQLIAEQSGGSVEPAASREYG
                     RANFTMCNNNKELLNGISVGSQVWMSHGDTITRIPDNFEIIASTSDVKIAAYKVSGEN
                     TYGVQFHPEVFHSTDGTHLLDNFLNICGCKKDWSAASFIETTVENLKNQLGDDKVVLG
                     LSGGVDSSVSAVLLNKAIGKNLTCIFVDHGLLRKNEFENVLRDYESLGLNVIGVNAKN
                     RFYSELSGVKDPEKKRKIIGKGFIDVFDEEAKKIKNVKWLAQGTIYPDCIESLSITGT
                     TIKSHHNVGGLPEKMNLKLCEPLRLLFKDEVRKVGTQLGMPQHLIKRQPFPGPGLGVR
                     ILGDITEEKVRILQDADDIFIKALHEWNLYDSVWQAGVILLPVQSVGVMGDERTYENA
                     VALRAVTSIDAMTADWAHLPYDFLAKVSNDIINKVKGVNRVTYDISSKPPATIEWE"
     gene            2847..3260
                     /locus_tag="PK641_05330"
     CDS             2847..3260
                     /locus_tag="PK641_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HPG55803.1"
                     /translation="MKTKLLFISLLLAATSSLFAQDAVETLKFKEKIHDFGQIKEEGG
                     KVTTTFEFTNTSKKPITLTNVTASCGCTTPEWTKEPVAPKKKGFINVTYNPAGRPGNF
                     TKSITIKFTEAGNETAQVAILNIKGEVIPKPKDKQ"
     gene            3354..3812
                     /locus_tag="PK641_05335"
     CDS             3354..3812
                     /locus_tag="PK641_05335"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HPG55804.1"
                     /translation="MKRVIYFTIGLILLSTISGFAQMSLAFDNKEYSFGNLSETDTLL
                     TYSFVFCNTGQDHAIIKRVESSCNVIVNYSKEAIKPNISGTIDILLDVSQTIGKFNKS
                     IYIYGDTAAIQLSITGNRIAAPTYKDEDYSAIPPTSNKDKKATKKRNKKK"
BASE COUNT         1428 a          673 c          648 g         1135 t
ORIGIN      
        1 tatcatataa agacggaaat aaaaacaaat cggcagcagc ataatatacc tgcagtgcag
       61 acctatcttg caattgacca gtcataaaaa ttttatcttg caaaccatat tgtttgatct
      121 tttcatgaaa ttctattgca gcataaccct tgcccacaaa atacattttg tacggcacat
      181 cttttagtaa tttaacagaa tccaatatgt attgaatatt cttttcgtaa ataatttgcc
      241 ctacaaataa caaacatatt tcattgtctt taagccccaa cttgcatctc gattcagcct
      301 tcaaaatatt aatatcctga ttacccaaat cgtaatctat cgcattcgga accacttcta
      361 ctttcccttt ataaccatat tcgcgcataa catcaagtac tgccggttgt ggaatccaaa
      421 cttcatcagc gtattcgtac aaactcatta ttcgctttat cattaaatca acaatggtat
      481 cagaaggtac acttctacga aaatcatctc taaatttcga atgaaatgtc ataatgaacg
      541 gagcattgta tttcctcgaa agtttcaatg caatacgacc tgctgtgaag ggtgaatgcc
      601 catgaaatat atcggcatta tatttcataa tcggctgaaa tggagccatc caaataggca
      661 caccaaaacg atatggcata cgcatcaaaa caggaatcga taaaaaatta cgaacgtgat
      721 atggttgtga gtaatcaaaa aacggcactt ttgcagttgc aacagttacg tcattccctc
      781 tctcttggag taaccttgca taattttcaa caacctgtgc cactccatcc tgaataggag
      841 gaaagcactc attaaataaa cctattttta acgaagaatt attcattcat acagaagttt
      901 cgttttttgt tatgccaaag gtaaataaag tttcagttat acacaaaaaa atcttccatg
      961 tcggcacccc tacacgacat ggaagaagta ccacgagaat ggtactgaaa atatttttgt
     1021 ttttactcgt tttctttatt tcgagttaaa gttattgtat atttttggta caacaatagt
     1081 acaaacttac aaatttacta tttttaagtt ttaatttcta tttttaccaa aatcagcatc
     1141 atgattccta aaaaaaccca tgactaaaca tgtataacaa atatattttg gctatttttg
     1201 tgcggtaatt aaatacacaa ataaaactca tatatgctgg aaaagattgt tattttagat
     1261 ttcgggtctc aaacgactca actcattgca cgacgcgtgc gcgagttaaa cacatactgc
     1321 gaaatactac catacaataa gtacccacaa aatgacagca ctataaaagg tgtaatcttg
     1381 tccggaagtc cattttcagt ttacgacaaa gctgccttta aaacagacct aagacaattt
     1441 gtaggcaaat tacctgtttt aggattatgc tatggcgcac aattaattgc agaacaatca
     1501 ggtggatctg tagaacctgc agctagtcga gaatatggcc gtgcaaactt tactatgtgt
     1561 aataacaaca aagaattgct taatggtatt tcagttggtt cacaagtatg gatgtcgcat
     1621 ggagatacaa taacaaggat acccgataat ttcgaaataa tagcgagcac gtctgatgta
     1681 aaaatagctg cttataaagt gagtggagaa aatacatacg gagttcaatt tcaccctgaa
     1741 gtatttcatt caacagacgg aacacattta ctagacaact tcttgaacat ctgtggatgc
     1801 aaaaaagatt ggtcagcagc ttcattcatc gaaacaactg ttgaaaattt aaagaaccaa
     1861 ctaggtgatg acaaggtagt tcttggttta tcaggaggtg ttgatagttc agtatcagcc
     1921 gttttgctta ataaagctat tgggaaaaac ttaacttgca tcttcgtgga ccatggtttg
     1981 ttgcgcaaaa acgaatttga aaatgtacta cgcgattacg aaagcttagg tcttaatgta
     2041 ataggcgtca atgccaaaaa tcgtttctat agtgaactat caggagtaaa agaccctgag
     2101 aaaaagcgta agataatagg taaaggtttc attgacgtat ttgatgaaga agcaaaaaaa
     2161 ataaaaaatg taaagtggtt ggcacaaggc actatatatc cagactgcat tgaatcacta
     2221 tcaataacag gaacaactat taagtctcat cacaatgttg gcggacttcc tgagaaaatg
     2281 aatctaaaat tatgcgagcc tttaagatta ctatttaaag atgaagttcg taaagttgga
     2341 actcaattag gtatgccaca acatttgatt aaacgtcaac cattccctgg accaggttta
     2401 ggagtaagaa tacttgggga cattacagaa gaaaaagtaa gaattttaca agatgcggat
     2461 gatattttta tcaaagcact gcacgaatgg aatttatacg atagcgtatg gcaagccgga
     2521 gtaattctac ttcctgtaca atctgtagga gttatgggtg atgaacgtac atacgaaaat
     2581 gcagtagcac taagagccgt tacatcaata gacgcaatga cagcagattg ggctcacctt
     2641 ccgtatgatt ttttagcaaa ggtatctaat gatattatca ataaagtgaa aggtgttaac
     2701 agagtaactt atgacatatc ttctaagcca cctgcaacaa tagaatggga gtagtgttta
     2761 aaaaacttaa ataagaagat aacgagttta cttttggtat atctttgtgt ctttaagcag
     2821 taattttatt ttaaaacata cataatatga aaacaaaact tttattcatt agtttattat
     2881 tggcagctac cagtagttta tttgcccaag atgcagttga aactttaaag tttaaggaaa
     2941 aaattcacga ttttggacaa atcaaagaag aaggtggaaa agtaacaacc acttttgaat
     3001 ttacaaatac aagcaaaaag cctattacac taaccaacgt aacggcttct tgtggatgta
     3061 caactccgga atggacaaaa gaaccggttg ctcctaagaa aaaaggattc ataaatgtaa
     3121 catacaatcc tgcaggacgt cctggtaatt ttacaaagag cattactata aaattcacag
     3181 aagctggaaa cgaaaccgct caagttgcta ttttaaatat taaaggtgaa gttattccaa
     3241 aaccaaaaga taaacaataa tcagtaataa caaacacaaa ctaaatgcgg caaaattttc
     3301 attttgccgc atttattttt ttgggtatct ttacaaattc aaatcaaatt ttcatgaaaa
     3361 gagtaatata ttttacaatc ggactaattt tactttccac aataagtgga tttgcacaaa
     3421 tgtcacttgc atttgataac aaagagtatt catttggaaa tttatcagaa acagatacac
     3481 tactgacgta tagttttgtt ttttgcaata cgggtcaaga tcatgcaata attaagcgag
     3541 tagaatcttc ttgtaatgtt attgtaaact attcaaaaga agctataaaa cctaacatta
     3601 gcggcactat cgacatttta ttagacgttt cacaaacaat aggaaaattc aacaagagta
     3661 tctacattta cggagatact gctgccatac aattaagcat aacaggtaat cgaattgcag
     3721 caccaacata caaagatgaa gattattctg caataccacc aacaagcaac aaagacaaaa
     3781 aagcaacaaa aaagaggaat aagaaaaaat aagaagatca attattgtaa agctatggaa
     3841 cacatagaaa acgattcaaa atatgcaggt ttatcagtta ataa
//
LOCUS       DAOPID010000146         3870 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_32120_length_3870_cov_3.343119, whole genome shotgun sequence.
ACCESSION   DAOPID010000146 DAOPID010000000
VERSION     DAOPID010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32120_length_3870_cov_3.343119"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="PK641_05340"
     CDS             <1..813
                     /locus_tag="PK641_05340"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HPG55805.1"
                     /translation="CRVKLFGMKKIYTVCFILLSILCFSCVTPRKTHYLQDPDKGIAS
                     YPEVYTPADYLIQNSDELNIRVITLDPESKKIFNSSNSSSSNTGSSKGLYTYTVYEDG
                     TIDFPYVGTVPVSGKTTRQIKIALEDTLRSYIKDCSVEVRLVNSYFNLLTESKAARYK
                     IVKEKMTIFEALAVGGDLGQFSDRKRVKILRQQQNGETSMKEFDLRSKDIIGSEFYYI
                     QPNDIIYVKAFNGQFFRINSFLTALGVTTTTISFGLLIWSIVRLCLPATSTK"
     gene            786..1559
                     /locus_tag="PK641_05345"
     CDS             786..1559
                     /locus_tag="PK641_05345"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HPG55806.1"
                     /translation="MFTCNKHKIINYLAVVVLLLAANSCITNKSNTYLQDDPKLPQYE
                     KAEYNYYKIQPNDELIIRLISVNEEATAIFNISGSEGSGSNSYNYRVYDDGTIDLPFI
                     SQIKVAGLTIREAAKQIEARLKDFAPDAMVKIALANDNFYIVGEGGRGAYKLYKEKLN
                     IFQAISLSGDFSKNGDRKHVRIIRPNPEGGKPFIKEFDMRTASIIGSEFYYVQPNDVI
                     YVSSIKGDFFKVQDYSSTIGSFSTSIGFLVTVINLGLSF"
     gene            1573..>3870
                     /locus_tag="PK641_05350"
     CDS             1573..>3870
                     /locus_tag="PK641_05350"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HPG55807.1"
                     /translation="MDNNQQYTQNQYTSQANAFSDDDGGGQKFDIMLWVLRFLKSWYL
                     FVISLAIFLSIAYVKNRSWQPNYKSSALVIIEENKGLSGAQAALMQGFSVERGYRNVN
                     NQVIMFGSYELISRVIAKQPELTIDYYTQGRFKSTNLYKAAPILITKTFVSPQAYERE
                     FTIQDLGNNNFEISAPGTKQLPELKISGKYGIPIQNSSFFIQVDKSAIYRRNFNICFK
                     LLNPENLAITYSAKLSFDFLMTGASVVQASISGTVPERDCDFLNGLCEEFLADNLARK
                     NDAAIKTIEFIDEQLSTIADSLRISEGRLKDYKANNFVVASVGGSNLMGQYTQLDAMQ
                     TDLRLKESYLNYLTNYLKSNVEEGAIIAPDNLGVSDANLSSLVSQFTEMQLKRKEIGV
                     KSPLYAKYTRDIDAVKAQMYEALKNIRVGLDIQKADMQNRLQKLSAGMQELPYKEQQL
                     LNIQRKFKINDDYYTFLVQKRADAQIQKASNSPDNIILDKARLASMTNAGEKNKAYTM
                     FLAIGLLVPVAIIVLLELMVPYIRTESEVISMAPKQYQLLGVIMHTDRKTPVIVDKYP
                     KSAITEAFRVIRTRIEFLSGKSKGISVMVTSSQSGDGKSYISANIAGVFAMTGRKTIL
                     VDLDMRKPSINAELYLSANKGISSYLVNQISLEDAIIKHPDYKFDILPVGVVPPNPGE
                     LIKSDNMNNLISILKETYDYIVFDTSPIGLVTDAYGIIESVDIILYAVRAEQTNKSFY
                     KKAMSQLEANNVKKVGLIFNDVNLKK"
BASE COUNT         1296 a          622 c          690 g         1262 t
ORIGIN      
        1 tgccgagtta aattgtttgg aatgaagaaa atctacactg tttgctttat tttattgagc
       61 attttatgtt tttcgtgtgt aactccacga aaaactcatt atcttcaaga tcctgataaa
      121 ggcattgctt catatcctga agtatacacc cctgccgact atcttataca aaatagtgat
      181 gaattaaata ttcgtgtaat taccctagat cccgaatcaa aaaaaatatt caattcgtca
      241 aattcatctt catctaatac aggatcttct aaaggcttat atacttatac cgtttacgaa
      301 gatggaacta tcgatttccc atacgttggt actgtaccgg tatccggtaa aacaaccagg
      361 caaattaaaa ttgctctcga agacacattg cgtagctata taaaagattg ttctgtcgag
      421 gtgcgtttgg taaatagtta ctttaatttg cttaccgaat ctaaagctgc acgttacaaa
      481 atagttaaag aaaaaatgac tatatttgaa gcattggcgg tagggggaga cttaggacag
      541 ttttcagatc gtaaaagagt taagatactt cgccaacaac aaaacggcga aacctctatg
      601 aaagagtttg atttgcgtag taaagatatt ataggttccg aattttatta tatccagcca
      661 aacgatatta tttatgttaa agcattcaat ggtcagttct ttaggattaa ttcattcctt
      721 actgcacttg gagttacaac tacaacaata tcatttggtt tattaatatg gagcatagtt
      781 cgcttatgtt tacctgcaac aagcacaaaa taataaacta tcttgcagtc gttgttttat
      841 tgttggcagc aaactcctgc ataacaaata aaagcaatac ctatttgcaa gacgatccca
      901 aactgcctca atacgaaaag gcagaatata actattataa aattcagcct aatgacgaat
      961 taataatacg tcttatcagt gttaatgaag aggctacagc aatttttaat atttccggtt
     1021 ctgaaggttc cggttctaat tcatataact atagagttta tgatgacggg acaattgatc
     1081 taccctttat atcgcaaatt aaagttgccg gcttaactat aagagaggct gcaaagcaaa
     1141 ttgaggctag acttaaggat tttgctcccg atgctatggt taaaattgct cttgctaacg
     1201 acaatttcta tatagtaggc gagggtggtc gtggtgcata taaattatac aaggaaaaac
     1261 taaatatatt tcaggctatt tcgctttctg gtgatttttc taaaaacgga gatagaaaac
     1321 atgtacgcat tatccgtcct aatcccgaag gaggaaaacc atttattaaa gaattcgata
     1381 tgcgtacagc atctattatt ggctctgaat tttattatgt acagccaaat gatgttattt
     1441 atgtatcatc cattaaggga gattttttta aggtgcaaga ttattcatca acaatcggtt
     1501 cttttagtac atcaataggt tttcttgtaa ctgtaataaa tttaggctta agtttttaaa
     1561 taaaaaattt aaatggataa taatcaacaa tatacacaaa atcagtatac atcacaagca
     1621 aatgctttta gtgatgacga tggtggcggt cagaagtttg acataatgct atgggtgctt
     1681 cgctttctta aatcatggta tctgtttgtt atatccttag ctatattttt gagtatagct
     1741 tatgtaaaaa accgcagttg gcaaccaaat tataaatcat cggctttagt tattattgaa
     1801 gaaaataaag gtttaagtgg cgcacaagct gcattaatgc aaggctttag tgtagaaagg
     1861 ggttatcgta atgtaaacaa tcaggttatt atgtttggct cttatgagct aatatctcgt
     1921 gttatagcca aacaacccga acttaccatt gattactata ctcaaggcag gtttaaatcc
     1981 acaaatttgt ataaggcggc acctatttta attacaaaga cctttgtatc acctcaagct
     2041 tacgagcgtg agtttactat tcaagatctt ggtaataaca atttcgagat tagtgctccc
     2101 ggaacaaaac aactacctga gcttaagata agcggtaaat atggtattcc tattcagaat
     2161 tcatcatttt ttatacaggt agataaatct gcaatttatc gccgtaattt taatatatgc
     2221 tttaagctgc ttaatcccga aaatcttgcc attacatatt cagctaagct atcttttgat
     2281 tttcttatga caggagcttc tgtagtgcaa gcttccatta gcggtaccgt tccggagcga
     2341 gattgtgact ttttaaacgg cttatgcgaa gaatttcttg ccgataatct tgctcgtaaa
     2401 aatgatgctg ctataaaaac catagaattt atagatgagc aattgtcaac tatagccgat
     2461 tctcttcgta tatcagaagg acgtttaaaa gactataaag ccaataattt tgttgtagca
     2521 tcagtaggtg gctctaatct tatgggtcaa tatactcaat tagatgccat gcaaacagat
     2581 ttgcgtctta aagagtcata tcttaattat cttaccaatt acttaaaaag taatgtagaa
     2641 gaaggtgcaa ttatagcacc tgataattta ggtgtgagtg atgctaattt atcatctctt
     2701 gtaagtcaat ttacagagat gcaattaaag cgtaaagaaa ttggggttaa gagtccctta
     2761 tatgcaaaat acactcgaga tattgatgct gttaaagcac aaatgtatga ggctttaaaa
     2821 aatattcggg ttggtttgga tattcaaaaa gcagatatgc aaaatcgttt acagaaactt
     2881 agtgcaggta tgcaagaatt gccatacaag gagcagcaat tacttaatat tcagcgtaag
     2941 tttaagatca atgacgatta ttatacattt ttagtccaaa aacgtgctga tgctcaaatt
     3001 caaaaagcct caaattcgcc tgacaatatt atattggata aagccaggct tgcatccatg
     3061 acaaatgcag gtgaaaaaaa taaggcttat accatgttct tagcaatagg cttattagtt
     3121 cctgttgcaa ttatagtatt gcttgaatta atggtgccat atattcgtac cgagtccgaa
     3181 gttatttcta tggcacctaa acaatatcag ttattaggtg taatcatgca tactgataga
     3241 aagacacctg taatagttga taaatatcct aaatctgcta taactgaggc atttagagtt
     3301 attcgtactc gaatagaatt tctttctggt aagtcaaaag gaatatctgt gatggtaaca
     3361 tcatctcagt ccggtgatgg caaaagttat atctctgcta atattgcagg agtatttgcc
     3421 atgaccggtc gtaaaacaat attagtcgat ttggatatgc gtaagccaag tattaatgca
     3481 gagctttatt tgtctgcaaa taagggtata agtagttatt tagttaatca aataagttta
     3541 gaagatgcta taatcaagca tcctgattat aaattcgata ttctgcctgt aggtgtagta
     3601 cctccaaatc cgggtgagct tattaagtcg gataatatga ataatcttat atcaatttta
     3661 aaagaaacat acgactatat agtttttgat acatctccta taggcttagt aaccgatgct
     3721 tatggtataa tagaatctgt tgatattatc ttgtatgcgg ttcgtgccga acaaaccaat
     3781 aaatcttttt ataaaaaggc aatgtctcag cttgaggcta ataatgtaaa aaaagttggc
     3841 ttaattttta atgatgtcaa tctaaagaaa
//
LOCUS       DAOPID010000147         3850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_32364_length_3850_cov_3.499605, whole genome shotgun sequence.
ACCESSION   DAOPID010000147 DAOPID010000000
VERSION     DAOPID010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32364_length_3850_cov_3.499605"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="PK641_05355"
     CDS             <1..621
                     /locus_tag="PK641_05355"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPG55808.1"
                     /translation="TEKTAYDPRSPYSASKASSDHFVRAYFHTYGLPVVISNCSNNYG
                     ANHFPEKLIPLAINNIKHNKPIPIYGKGANVRDWLWVEDHARAIDTIFHTGKTGETYN
                     IGGHNEWTNLDLIKLLCKVMDRKLGREEGTSEKLITYVTDRAGHDLRYAIDPTKLRDQ
                     LGWKPSLQFEEGLEKTVDWYLENEEWLDNVTSGAYQQYYEKQYKNC"
     gene            633..1802
                     /gene="pepT"
                     /locus_tag="PK641_05360"
     CDS             633..1802
                     /gene="pepT"
                     /locus_tag="PK641_05360"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HPG55809.1"
                     /translation="METDALERFLRYVTYDTQSNKDATDTPSTKGQFELARILEQELL
                     QLGLSKITLDKHCYLYGLLPANTNKTDISIGFIAHLDTSPAVSGKDVKPRIITNPEGE
                     QIIVSDGTTLLGADDKAGIAAIVSAMAYLQAHPEILHGNIYVAFTPDEEIGRGTNFFN
                     TKIFKADWAYTVDGGEIGELEFENFNAAKAILIINGKNVHPGYAKGIMKNSMLIAGKV
                     IEELSKEDIPENTEGRKGFYHLDFINGSVEQTVLHYIIRDFDIEEFDRKKKFLQYLEH
                     SINEQFGEGCASLVITDQYSNMGEIIKQHPHIIQIAQKAMEEIGVTPKITAIRGGTDG
                     ARLSFMGLPCPNLFAGGMLFHSKDEYLPVKSLQKACETIIAIIKNTAEYNKKCIS"
     gene            1939..3012
                     /gene="serC"
                     /locus_tag="PK641_05365"
     CDS             1939..3012
                     /gene="serC"
                     /locus_tag="PK641_05365"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HPG55810.1"
                     /translation="MKKHNFNAGPSVLPREVIEKTAQAVLDFQGEGLSILEISHRAKN
                     FQPVVDEAVALFKELLNIPEGYSVIFLGGGASLQFCMVPFNLLEKKAAYLNTGVWAKK
                     ALKEAKGFGEVVEVASSADANYTFIPKGFTIPTDADYFHITTNNTIYGTEILKDLDSP
                     VPLIADMSSDIFSRPVDVSKYGMIYGGAQKNLAPAGVTFVIIKNDLLGKVSRYIPTML
                     NYQTHINEGSMFNTPPVLPIYTALQTLKWLKENGGLKEMEKKNIAKANLLYNAIDNSK
                     MFVGTANKEDRSRMNICFVMKEEYKALEDDFLKFAVSKGMVGIKGHRSVGGFRASCYN
                     ALPIESVQALVDCMAEFEKQNAK"
     gene            3014..>3850
                     /locus_tag="PK641_05370"
     CDS             3014..>3850
                     /locus_tag="PK641_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPG55811.1"
                     /translation="MKVLVATDKPFAKVAVDGIKAVLDKAGYELALLERYTEKKQLLD
                     AVADANAIIVRSDIIDAEVISAAKNLKIVVRAGAGFDNVDLEAATKAGVCVMNTPGQN
                     ANAVAELAFGLMVYAIRNFYNGTSGTELMGKKLGIHAYGNVGRNVARVAKGFGMEIYA
                     FDAYCPAEVIEKDGVKALKSVEELYSTCQFISLHIPATAETKNSINNSLLSRMPKGAL
                     LLNTARKEVIDEAGLIKTMEERPDFRYITDILPAANEEFAGKFPGRYFSTPKKMGAQT
                     AEA"
BASE COUNT         1341 a          650 c          748 g         1111 t
ORIGIN      
        1 accgaaaaaa cagcatacga cccacgcagc ccatattcag cttcaaaagc aagttcagac
       61 cattttgttc gtgcctattt ccacacttat ggtttacctg tagtaatttc caattgctca
      121 aacaattatg gtgcaaacca tttccctgaa aaattaattc cacttgcaat caataacatc
      181 aaacacaaca aaccaattcc tatttacggt aaaggagcta atgtacgtga ttggttatgg
      241 gttgaagatc atgcacgcgc tattgatacc attttccaca ccggaaaaac aggtgaaaca
      301 tacaacatag gcggtcacaa cgagtggacc aacttagacc ttatcaaatt actttgtaaa
      361 gtgatggatc gcaaattagg acgcgaagaa ggcacatccg agaagctaat aacatacgta
      421 actgatcgcg caggacacga cttacgctat gctattgacc ctaccaaatt acgcgatcaa
      481 ttaggttgga aaccatcgtt acaatttgaa gaaggcttag agaaaactgt tgattggtac
      541 ttggaaaatg aagaatggct tgacaatgta acaagtggcg catatcaaca atactacgaa
      601 aaacaatata aaaactgctg atactataaa atatggagac tgatgcatta gaaagattct
      661 tacgttatgt tacgtacgat actcaatcga ataaagatgc aacagatact ccaagtacca
      721 aaggtcaatt tgaattagca cgcatattgg agcaagaatt gctccaacta ggtttgtcta
      781 aaattacttt agataaacat tgttatttat acggattact gcctgcgaat accaataaaa
      841 cagatataag tatagggttc atcgcgcact tagacacgag tcctgccgtt tcgggcaaag
      901 atgttaagcc acgcattata actaatcccg aaggagaaca aataattgtt tctgatggaa
      961 caactttact cggtgctgat gataaagcag gcatcgcagc tattgtatca gcaatggcat
     1021 atttacaagc acatcccgaa atattacatg gtaatatata tgtagccttt actcctgatg
     1081 aagagattgg aagaggcaca aactttttta atactaagat atttaaagca gattgggctt
     1141 atacggtaga tggaggcgaa ataggcgaac ttgaatttga aaatttcaat gcagcaaaag
     1201 ccattttaat catcaatgga aagaatgttc atccgggata tgcaaaaggc attatgaaaa
     1261 attcgatgct tatagcaggt aaagtaatcg aagaattgag taaggaagat atacccgaaa
     1321 ataccgaagg gcgtaaagga ttttatcacc ttgattttat taatggtagc gtagaacaga
     1381 ccgtactaca ttatataata agagattttg atattgaaga atttgacaga aagaaaaagt
     1441 tcctgcaata tcttgaacat agcattaatg agcagtttgg cgaaggatgt gcatctttag
     1501 ttataactga tcagtattca aatatgggag aaataatcaa gcaacaccca catattattc
     1561 agattgcaca aaaagcaatg gaagaaattg gtgttacgcc caaaatcaca gctattcgcg
     1621 gaggaactga cggagcgaga ttatctttta tgggacttcc ctgccctaat ttatttgcag
     1681 gaggcatgct ttttcatagt aaagatgaat atttgcccgt aaaatcctta caaaaagcat
     1741 gcgaaacgat aattgctatt atcaaaaata ctgctgaata caataaaaaa tgtattagtt
     1801 agaaacaaaa catacgtgtt gataaatatt cgtatatatt ttttcaatac aaaggaaaat
     1861 gtagtacttt tgtgggtcat tttcgtaaaa tttatagaaa acaacattat tagcatttaa
     1921 caccaataat ataattgtat gaagaagcac aattttaatg ccggaccttc agttttacct
     1981 agagaggtca ttgaaaaaac agcacaagca gttttggatt ttcaaggtga aggtttatcg
     2041 attttggaaa taagccatcg cgcaaaaaat tttcaacctg ttgttgatga agcggtagca
     2101 ttgttcaaag aattattaaa catacccgaa ggttactccg ttattttctt aggtggtggt
     2161 gccagcttac aattttgcat ggttccattt aacttactag agaaaaaagc tgcatatcta
     2221 aatacaggtg tatgggcaaa gaaagctttg aaagaagcta aaggatttgg agaagttgta
     2281 gaagttgcct cttctgcaga tgctaattac acttttattc ctaaaggttt cactatccct
     2341 actgatgcag attatttcca catcacaacc aataatacca tttacggtac cgaaatctta
     2401 aaagatttag attctcctgt acctttaata gctgatatgt cttctgatat attctcaaga
     2461 cctgttgacg ttagcaaata tggtatgatt tatggtggtg cacaaaagaa tcttgctcct
     2521 gcaggtgtta cttttgttat cattaaaaat gatttgttag gcaaagttag ccgttacatc
     2581 cctactatgc tcaactatca aacacatatt aatgaaggtt ctatgtttaa tactcctccg
     2641 gtattaccta tctacacagc tttacaaaca ttaaaatggt tgaaagaaaa tggtggattg
     2701 aaagaaatgg agaagaaaaa cattgcaaaa gctaacttat tatataatgc tattgataat
     2761 agcaagatgt ttgttggcac agcaaataaa gaggatcgtt ctcgtatgaa catctgcttc
     2821 gttatgaaag aagaatacaa agctcttgaa gatgatttct tgaaatttgc cgtatcaaaa
     2881 ggaatggtcg gaattaaagg acatcgttct gtaggtggat ttagagcatc atgctacaat
     2941 gcacttccta ttgaaagtgt acaagcttta gttgattgta tggctgaatt cgaaaaacaa
     3001 aatgctaaat aaaatgaaag tacttgttgc aacagataag ccgtttgcca aagtggctgt
     3061 tgacggtatt aaagcagtgc ttgataaagc cggatacgaa ttggctttgt tagaaagata
     3121 cactgaaaaa aaacaattac ttgatgcagt tgctgatgca aatgccatca tcgtacgtag
     3181 tgacattatt gatgccgaag ttatttcggc tgctaaaaat cttaagattg ttgtacgtgc
     3241 aggtgcaggt ttcgacaatg ttgatttgga agctgcaaca aaagcaggtg tatgtgtaat
     3301 gaatacaccg ggacaaaacg ctaacgcagt agctgaatta gccttcggat tgatggtgta
     3361 tgctatacgt aatttttaca atggtacatc gggcacagaa ttaatgggaa agaaattagg
     3421 tattcatgct tatggtaacg ttggtcgcaa cgtagcacgt gttgctaaag gatttggaat
     3481 ggagatctat gcatttgatg catattgccc ggctgaagtt attgagaaag atggtgttaa
     3541 agcacttaaa tcggtagaag aattatattc aacatgccaa tttatttctt tacacattcc
     3601 tgcaactgca gaaactaaaa actcaatcaa taactcttta ttatctcgta tgcctaaggg
     3661 agcattatta ttgaatactg ctcgtaaaga agttattgac gaagccggat taatcaaaac
     3721 catggaagaa cgtcctgatt tccgttatat tacagatatc cttcctgctg ctaatgaaga
     3781 atttgcagga aaattcccgg gacgttattt ctctacacct aaaaagatgg gagcccaaac
     3841 agcagaagca
//
LOCUS       DAOPID010000148         3844 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_32437_length_3844_cov_5.170757, whole genome shotgun sequence.
ACCESSION   DAOPID010000148 DAOPID010000000
VERSION     DAOPID010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32437_length_3844_cov_5.170757"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..446)
                     /locus_tag="PK641_05375"
     CDS             complement(84..446)
                     /locus_tag="PK641_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HPG55812.1"
                     /translation="MMKKYVYVFCGTISLCLGIIGIILPVLPTTPFLLLTAWLYFRSS
                     PKLYQKLMDNKYFGPYIKRFREDKAIPLHAKIVSISLLWITISYSAICIVDELWLKIL
                     LFAIAIAVSIHILSFKTK"
     gene            621..1538
                     /locus_tag="PK641_05380"
     CDS             621..1538
                     /locus_tag="PK641_05380"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-dehydropantoate 2-reductase"
                     /protein_id="HPG55813.1"
                     /translation="MKYAIVGTGAIGGFYGGELARIGKDVHFLLRSDYEFVKQNGLKV
                     HSVNGDFCINPINAYNDAKQMPQCDIILVCMKTTQNHLLPELLYPLVKPSTCIVLVQN
                     GLNIEKKLASSYPDIDIAAGMAFVCCNKIKIGEIMHLDYGKLTVSPYQGKCSYLKTLC
                     NDLTEAGIDNYLWEEYVTARWRKLVWNIPYNGLTVVLNTTTELLMKNEASRQLVKELM
                     EEVRAGGLACGADIPDNFIDEMLKSTDQMRPYDPSMRLDFLRHHQMEIEAIYSNPIAA
                     AHNAGVEMPKIAMLEKQLKFIQAGYLQKV"
     gene            complement(1541..1981)
                     /gene="mscL"
                     /locus_tag="PK641_05385"
     CDS             complement(1541..1981)
                     /gene="mscL"
                     /locus_tag="PK641_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HPG55814.1"
                     /translation="MNQLIKEFKDFAVKGNMIDMAVGIIIGGAFGKIITSLVNDVIMP
                     PIGLLVGGVNFTDLKWDMKDAVVDAAGVITPAVTLNYGNFLQVLFDFLIVAFAIFMFV
                     RLISKAKQRTETPAKPAAPEAPAPPTKEETLLTEIRDLLKQSKK"
     gene            complement(2058..3161)
                     /locus_tag="PK641_05390"
     CDS             complement(2058..3161)
                     /locus_tag="PK641_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032528339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HPG55815.1"
                     /translation="MKTKDSKVSHKSSDLTSVLVLHFGKSLNLARIKFISLFICALCK
                     VQVVCFEKLASGFENGCGSDSSLRRIQRFMADYKLDKDLIARLIFALLPHEPPYSIAI
                     DRTNWKFGQTNINILVLAIVYKGVAFPILFKLMPKRGNSHTAERIQIVNHYIRLFGKQ
                     TIQYLVADREFVGEHWISYLNFNRIEYHIRIRDNFWVLNPKTGKQFKATWLFSDLKLN
                     ETRFLHSIYNIHNQLCYLSASKIKNKEGKPEFQIVISFCKPEIAQKTYKERWQIETAF
                     RAMKTSGFNIEDTHLTDIDRIEKLVALVTIAFCWAYLVGIYLHEHDKPIRILNNGRMA
                     KSFFKYGLTFIALVLLNAGFQSDIDIFKFLSCT"
     gene            3477..3641
                     /locus_tag="PK641_05395"
     CDS             3477..3641
                     /locus_tag="PK641_05395"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HPG55816.1"
                     /translation="MNPIEIDVVVAHVDVDSMQPVFFNDEIAVQTTVEKIENKSFTLC
                     QRIIDLKNNV"
BASE COUNT         1362 a          654 c          614 g         1214 t
ORIGIN      
        1 tgtgcataag caatgcttcg actatactct ataaaaatta ctacaccaat acgcaagaat
       61 gcaaaaaaaa tacgaaagca atactacttt gttttaaatg acaggatatg aatgctcaca
      121 gctattgcaa ttgcaaaaag taaaattttc aaccataatt cgtctactat acaaatagcc
      181 gaatagctga tagtgatcca caatagcgat attgaaacga ttttggcatg aagtggaatt
      241 gccttatcct cacgaaacct tttaatatat ggaccaaaat atttgttgtc cataagtttt
      301 tgatataatt taggtgagct acgaaaatat agccatgcag ttaatagaag aaaaggtgtt
      361 gtgggtaaaa caggtaaaat gatgccgatt atgcctaagc ataatgaaat tgttccgcaa
      421 aacacataaa catatttctt catcattatc tcttttctta tttttctaca aaaatattca
      481 gcaaagatat gaaatattga aacaccacat caactataga acttatagaa ttgagcatcg
      541 ccttaaatat ttattttttc atggttttat tattacatct ttaacaacat atatttaact
      601 ttgcactaaa ttacagagat atgaaatatg caattgtagg gacaggtgcc attggaggat
      661 tttatggagg agagctggct cgaataggta aagatgtcca ttttttatta cggagtgatt
      721 acgagtttgt aaaacaaaat ggtttaaaag tacattcggt aaatggagat ttttgtataa
      781 atcccataaa tgcatataat gatgcaaaac agatgcccca atgcgacatc atattagttt
      841 gcatgaaaac tactcagaat catcttctac ctgaattgct atatccttta gtaaaaccaa
      901 gcacatgtat tgttctagta caaaatggct taaatattga aaaaaaatta gcctcatcat
      961 atcccgatat cgatatagca gccggtatgg catttgtatg ctgcaataaa ataaaaatcg
     1021 gtgaaattat gcatcttgac tacggtaaac tcacagtatc tccatatcaa ggaaaatgtt
     1081 catatttaaa aacattgtgc aatgacttga ccgaagcagg tattgataat tatctgtggg
     1141 aggaatatgt gaccgctcga tggcgcaaac ttgtatggaa tataccatat aatggactta
     1201 cagtagtact taacactacc accgaattac ttatgaaaaa cgaagcaagt agacaacttg
     1261 ttaaggaatt aatggaagaa gttagagccg gaggcttggc ttgtggagct gatataccgg
     1321 ataactttat agatgagatg cttaagtcga ccgatcaaat gagaccatac gacccaagta
     1381 tgcgtttaga tttcttacgt catcatcaaa tggaaataga agcaatctac agcaatccaa
     1441 ttgctgctgc tcataatgcg ggagtagaaa tgccaaaaat agccatgctc gaaaaacaat
     1501 taaagtttat tcaagcaggc tatttgcaaa aagtttaaat ctatttcttg ctttgtttta
     1561 gcaagtcacg tatttcggta agtaatgttt cttcttttgt aggaggagca ggagcttcgg
     1621 gagctgcagg ttttgcagga gtttccgttc tttgttttgc tttactaatt aagcgaacaa
     1681 acataaatat tgcaaatgct actattaaaa agtcgaatag cacttgtaaa aagttaccat
     1741 agttaagagt tactgccggt gttataacac ctgctgcatc tacaacagca tctttcatat
     1801 cccattttaa atctgtaaaa ttcacaccac caactaacaa accaattgga ggcataatta
     1861 cgtcatttac taaagatgta ataatcttac cgaatgcacc gccaatgata ataccgacag
     1921 ccatgtcaat catattaccc tttactgcaa aatctttaaa ttctttgatt aattgattca
     1981 tagttaaaaa tttcaaatta ttaatttatt caacctgcac aaatatatac aacttaaatt
     2041 aattaatacg aatttttcta agtacatgac aaaaatttaa aaatatctat atctgattga
     2101 aaaccagcat ttaaaagcac caatgcaata aaagtgagac catatttgaa gaaacttttc
     2161 gccattcttc cattgttgag tattctgatt ggcttatcat gttcgtgtaa ataaattcca
     2221 accagatatg cccaacaaaa tgcaattgtt accaaagcaa caagtttttc aattctgtca
     2281 atatcagtca aatgagtgtc ttctatattg aatccacttg ttttcattgc tctaaatgcc
     2341 gtttcaatct gccagcgttc tttgtatgtt ttttgcgcaa tttcaggttt gcaaaatgag
     2401 ataactattt gaaattcggg tttcccttct ttgtttttta tttttgaagc agacaagtaa
     2461 cacaactgat tatgaatgtt ataaatcgag tgtaagaatc ttgtttcatt caattttaaa
     2521 tcactaaata gccatgttgc cttaaactgt tttccggttt taggattcag aacccagaaa
     2581 ttatcccgaa tacgaatgtg atattcaatc cggttaaaat tcagataact aatccaatgt
     2641 tcaccaacaa attcacgatc tgccaccagg tattgaatgg tttgtttccc aaataaccga
     2701 atataatgat tcacaatttg aattctctcg gctgtgtgag aattgcctcg ttttggcatt
     2761 aatttgaata aaattggaaa ggcaactcct ttataaacaa tggccaagac caaaatattg
     2821 atatttgttt gtccaaattt ccagttcgtt ctgtcaatag caatcgagta aggtggttca
     2881 tgtggcaaaa gtgcaaatat caatcttgca atcaagtctt tgtcaagctt gtaatcagcc
     2941 ataaaacgct gtattcgtcg cagtgaagag tcgctgccac atccattttc aaagccactg
     3001 gcaagttttt caaagcaaac gacctgaacc ttgcaaagag cgcaaataaa caaacttatg
     3061 aactttattc gggctaagtt taaactttta ccgaagtgta gaaccaaaac tgaggttaaa
     3121 tcactacttt tgtgcgagac cttggaatct tttgttttca ttataactaa ctggtaatca
     3181 gttataaaga tacaaaattt caaggtcttt accaaataaa aaggcagtta ttttgctgat
     3241 tatcaaataa ttatatcact atataatttt tgtcatgtac taaacgaatt tttatatgtt
     3301 tttttgaaaa ccgaatttaa tagttgtcta cattattgca aattcaatta tagcacacaa
     3361 taaacactat atttgcactt tcaatatata caaatgaata cagattttat tacgccgcca
     3421 aatcacacca atttctaggc aaaacaaact atttcaacgc tataagagga cgcaacatga
     3481 atcctatcga aatagacgta gtggttgcac atgtagatgt agattccatg caacctgtat
     3541 tttttaacga tgaaatagca gtacaaacaa cagttgagaa aatagaaaat aaaagtttta
     3601 cgctctgcca gcgcataatt gatctgaaaa acaacgtata aaaatgcgta tgccgtacag
     3661 ttatggctgg ctttgatttt aaaaacaact gtacaaaaac aatttccgaa cagtggagaa
     3721 aagacattga agaatacaaa aacaattaaa aaagtaagaa cttgcttttt aattgttgac
     3781 ttgcttttta tgcaacaaaa aaaatgcgtt actacttagt aacgcatttt ttataagtag
     3841 aaag
//
LOCUS       DAOPID010000149         3841 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_32479_length_3841_cov_3.699947, whole genome shotgun sequence.
ACCESSION   DAOPID010000149 DAOPID010000000
VERSION     DAOPID010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32479_length_3841_cov_3.699947"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PK641_05400"
     CDS             complement(<1..285)
                     /locus_tag="PK641_05400"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899292.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HPG55817.1"
                     /translation="MPDIKPSEISSVLQMELEGFKNKLTFEEIGRVLQVSDGVARIFG
                     LNQVQANELVEFENGTMGIVQNLEEDNVGVVLLGATQDIKEGFTVKRTKRI"
     gene            complement(272..808)
                     /locus_tag="PK641_05405"
     CDS             complement(272..808)
                     /locus_tag="PK641_05405"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HPG55818.1"
                     /translation="MNNGKISTRYARALLYSAKEKHSEDKVYIDICLLNKAFSDFPEL
                     KHAISSPTITDEDKMKLLTTAVGKDANAETLLFIKFIIQKKREDYMPFIGLMYERLYH
                     EEKRIVLSTVTTAVEIPKETASAITSYISKLRNATVELRTKTDSSIIGGFILDIEDNR
                     MDASIKGQLSKLYKYAGH"
     gene            complement(812..1318)
                     /gene="atpF"
                     /locus_tag="PK641_05410"
     CDS             complement(812..1318)
                     /gene="atpF"
                     /locus_tag="PK641_05410"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HPG55819.1"
                     /translation="MNLFLPESGLVIWMLLAFSIVFFVLAKFAWPSILKSIQQRQEYI
                     SDSLVKANEAIQTLSGLEQKGKQIIADAQAEQLRMVKETKALNDKMVAEAKEQAKIEA
                     EKIIENAHDRIRLDKEHAILEIRKEIAGLTISIAEKILKKELEDKQAQSQYINRLLKE
                     SADDIKAS"
     gene            complement(1385..1639)
                     /locus_tag="PK641_05415"
     CDS             complement(1385..1639)
                     /locus_tag="PK641_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HPG55820.1"
                     /translation="MLSIILQAAVAGASLAKLGACLGAAIAIIGAGLGIGRIGSTAME
                     AISRQPEAAGDIRSSMIVAAALIEGATFLAIIVCLLAIVL"
     gene            complement(1682..2824)
                     /gene="atpB"
                     /locus_tag="PK641_05420"
     CDS             complement(1682..2824)
                     /gene="atpB"
                     /locus_tag="PK641_05420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HPG55821.1"
                     /translation="MKKLYNILVCILILSFSCSVTYASESIQSDSIKTEKDSAKSLDV
                     SSILFGHIGDAYEYHITEIHEHPIAIPLPVIVYSKERGWFSFLSTKVTEGEAYNGFFI
                     QKGGSYDGKIVETNSKGEIVRPFDLSITKNVFALLFSCFLLIVIFVPMALSYKKRPLN
                     VPNKKQNLLEMVIMYIEDDVIKPSVGKKYLKYSPYLLTIFFFILINNLMGLIPIFPFG
                     ANLTGNISVTLILAMFTFFITNIFGTKEYWKEILWPDVPLMLKAPIPLMQMIEIISTL
                     TKPFALMVRLFANIFAGHMIILVLTTLIFLFSAVMGAAMGYATVVISVLFSVFMYCLE
                     LLVAFIQAYVFTLLSAIFIGLAQVEPHHKTKKEEILSVAQVNKKND"
     gene            complement(2835..3200)
                     /locus_tag="PK641_05425"
     CDS             complement(2835..3200)
                     /locus_tag="PK641_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55822.1"
                     /translation="MMKRYYYIVAISAVVLELIMTIIHQLVLKCNYSLDYLIVPLFFI
                     SAEFIYCKFVSMEKKTMSWFMIFKSLKLLVSIIILGLVYFVINKSDIGLYIRFFVAYF
                     VFLALETWIGLNYSKHKVR"
     gene            complement(3197..3442)
                     /locus_tag="PK641_05430"
     CDS             complement(3197..3442)
                     /locus_tag="PK641_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HPG55823.1"
                     /translation="MNVSIVAPNKTLYSGKAVAITVPGKNGSFTALENHAPIVSILDK
                     GTISVQAKAESEKVLFEIKSGFVEIHDNIVTICVEVA"
     gene            complement(3448..>3841)
                     /locus_tag="PK641_05435"
     CDS             complement(3448..>3841)
                     /locus_tag="PK641_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009227452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HPG55824.1"
                     /translation="PLDSTSRILDPNIVGEKHYKTALRVIELLQRYKELQDIIAILGM
                     EELSESDKIAVHRARRVQRFLSQPFHVAEQFTGLKGVMVPIEETIRGFNMIMDGETDK
                     YPEAAFNLVGNIDEAITKGEKLLFESNN"
BASE COUNT         1217 a          683 c          587 g         1354 t
ORIGIN      
        1 tattcgtttt gtacgtttta cggtaaagcc ttcttttata tcttgtgttg cgcccaataa
       61 aacaacaccc acattgtctt cttccaaatt ttgaacgatg cccattgtgc cgttttcaaa
      121 ttcaaccaac tcgttggctt gtacttgatt aagcccaaat attctggcaa caccgtcgct
      181 tacttgaagc actcttccta tttcttcgaa agtgagtttg tttttaaacc cttcgagttc
      241 catttgtaga acactcgata tttcgcttgg tttaatgtcc ggcatatttg taaagttttg
      301 ataattgtcc ttttatactg gcatccattc gattatcctc aatatctaag ataaaaccac
      361 cgatgatgct agagtcggtt tttgtacgta attctacggt agcattacgc aactttgaaa
      421 tatatgaagt tattgcactt gctgtttctt ttggtatttc aaccgcagtt gtaaccgtac
      481 tcagaacaat acgtttttct tcatgataca aacgttcgta cataagtccg ataaatggca
      541 tataatcttc acgttttttc tgaataatga attttataaa caataaagtt tccgcatttg
      601 catctttacc aactgctgta gttaaaagtt tcattttatc ttcatcagtt atagtaggac
      661 tgctaatagc atgttttagc tccggaaaat cagaaaaagc tttgttcaac aggcaaatat
      721 caatatatac cttgtcttca gaatgctttt ctttggcact atatagtaat gctcttgcat
      781 atcgtgtgga tattttaccg ttgttcatgg tttaagaggc ttttatatca tcagcagatt
      841 cttttaacaa gcgattaata tactgacttt gagcttgttt gtcttctaat tcctttttaa
      901 gaattttttc tgcaatacta attgttaaac ctgcaatttc ttttctgatt tctaatatgg
      961 catgttcttt gtctaaacgt attctatcat gtgcattttc gataattttt tcggcttcaa
     1021 ttttagcttg ttcttttgct tcagcaacca ttttatcgtt taatgccttt gtttctttta
     1081 ccatacgaag ttgttctgct tgtgcatcgg caataatttg tttacctttt tgctccaagc
     1141 cggacaatgt ttgtatcgct tcgttggctt ttaccaaaga atcagaaata tattcctgac
     1201 gttgttgtat agatttaagt atgcttggcc atgcaaattt tgctaataca aaaaatacaa
     1261 tggagaaagc taacaacatc catattacta aaccggattc tggaaggaat aaattcatgt
     1321 tatggttttt ttatgtgtcg agaattttaa actctcgaca catgattttt attactgcaa
     1381 taaattataa tacgattgct aacaagcata cgatgatagc aaggaatgta gcaccttcaa
     1441 tcaaggctgc tgcaacgatc atgctagaac gaatgtcgcc tgctgcttcc ggttgacgag
     1501 aaattgcttc cattgcagta ctaccaatac gaccgatacc aagacctgca ccaataattg
     1561 ctatagcagc tcccaaacag gcacctaatt ttgctaaaga tgcgccggct acggcggctt
     1621 gaagaataat agataacata actttagttt ttaaaaagtg aatactttat tattaatttt
     1681 actaatcatt ttttttgttt acttgagcca cagacaaaat ttcttctttt tttgttttat
     1741 ggtgtggttc aacttgggcc aatcctataa atatggcaga taacagagta aatacataag
     1801 cttgaataaa tgcaaccaat aactcaagac aatacataaa tacggagaat aacactgaaa
     1861 taacgactgt tgcatatccc attgcagcac ccataactgc agaaaataag aatattaaag
     1921 tagttaaaac cagaataatc atgtgtcctg cgaaaatatt tgcaaacaaa cgtaccatca
     1981 atgcaaaggg tttagttaga gtagatatta tttctatcat ttgcatcaat ggaatagggg
     2041 ctttaagcat aagcggaacg tccggccata atatttcttt ccaatattct tttgttccga
     2101 aaatatttgt aataaaaaac gtgaacattg ctaatatcaa agtaacagat atatttcctg
     2161 ttaggtttgc tccgaatggg aatattggta tcaatcccat taaattatta atcaggataa
     2221 agaaaaatat tgttagtaga tatggtgaat atttcagata ttttttgcct acagatggct
     2281 ttattacatc atcttcaatg tacataataa ccatttctaa tagattttgc ttcttgttag
     2341 gcacattaag tggacgtttc ttatacgaaa gcgccatcgg tacgaaaata acaatcagca
     2401 agaaacaaga aaaaagtagg gcaaatacat tttttgtaat tgataaatcg aatggtctta
     2461 caatctctcc tttcgaattt gtttcaacaa tttttccgtc gtaggagcct cctttttgaa
     2521 taaaaaatcc attatatgct tctccttcag ttactttggt tgataaaaaa gaaaaccatc
     2581 cacgttcttt actataaact ataacgggaa gaggaattgc tatagggtgc tcatgtattt
     2641 cagttatatg gtattcgtat gcatcaccta tatgtccaaa gaggatgctt gatacatcaa
     2701 ggctttttgc actgtctttt tcagttttaa tactatcaga ttgtattgac tctgaagcgt
     2761 atgtgacaga gcaagaaaaa ctcaatatca ggatgcatac taatatgtta tatagttttt
     2821 tcatgcaaga tacattatct aactttatgt tttgaataat ttaatcctat ccatgtttct
     2881 aatgccaaga aaacaaaata ggctacaaaa aatctaatat ataaaccgat atcgcttttg
     2941 ttgattacaa aataaaccaa tcctaatatt atgattgata caagcaattt caaacttttg
     3001 aaaatcataa accaagacat ggtttttttc tccatgctta caaacttgca atatataaat
     3061 tctgcggata taaagaacaa tggaacaatc aaataatcaa ggctataatt acacttaaga
     3121 accaattgat gtataattgt cattataagt tctaatacaa cagcagaaat tgctacaata
     3181 taataataac gtttcatcat gcaacttcaa cacatattgt aacaatattg tcgtgaattt
     3241 ctacaaaacc actttttatt tcaaataaca ctttttctga ttcagcttta gcttgaacag
     3301 aaatagttcc tttatccaga atagatacaa ttggagcatg attttccaat gcagtaaatg
     3361 aaccattttt acccggcact gtaattgcaa cagctttacc agagtataat gttttatttg
     3421 gagctactat ggatacgttc ataattttta attattactt tcgaataata atttttcacc
     3481 cttagtaata gcttcatcta tattaccaac taaattgaat gcagcttcag gatatttgtc
     3541 agtttcacca tccataatca tattaaagcc acgtattgtt tcttcaattg gaaccataac
     3601 accttttagt cctgtaaatt gctctgcaac gtggaatggc tgtgataaaa atctttgtac
     3661 acgtcttgct ctgtgtactg caattttatc tgattcagat aattcttcca tacctaagat
     3721 tgcaataata tcttgcaatt ccttatagcg ttgaagcaac tcgataacgc gtaaagctgt
     3781 tttatagtgt ttttcgccta ctatattagg atctaggata cgagatgttg agtctagcgg
     3841 a
//
LOCUS       DAOPID010000150         3828 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_32640_length_3828_cov_5.130400, whole genome shotgun sequence.
ACCESSION   DAOPID010000150 DAOPID010000000
VERSION     DAOPID010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32640_length_3828_cov_5.130400"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="PK641_05440"
     CDS             <1..571
                     /locus_tag="PK641_05440"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPG55825.1"
                     /translation="DTVEVSIVYMLLLMLLISIFIIKAIQTEPIELPEHCKISWSYKK
                     FQWIIVDELADCRYKTAEADKFSYLSSNELNENEINRYKSILDEWMLQDKPYLNKDFR
                     VSDVHAKLNIDRLKCNEIFSRAYKCYFRNWVLQYRIEYAIKLMKENPDKQVKEISFEA
                     GFTSQAIFARSFALQMGMSCTQYKERFLI"
     gene            1007..2404
                     /locus_tag="PK641_05445"
     CDS             1007..2404
                     /locus_tag="PK641_05445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HPG55826.1"
                     /translation="MQNKLILILLVFLSTSFSLYAKPSTGRIDSNIPKNWILKSDTFF
                     LEQQPIEQKWWQSFDDPVLNSIEELAIANNYDLVAASYRIAQAKANMRIAEAGFYPQI
                     SLGAAYDYNKNISAIGMIAHTGSLGLDFQWEIDIIGSVRNKANAQKKVYEATQEEYNA
                     MMTILVAQVADAYFSLRTTQLRLNVVKRNLASQKETMDITEARFNSGLVSALDVSQAK
                     SIYYSTAASKPKLESLVSKYINTIGVLVGKLPWDLESDLALVKDIPLKENPILVPVGI
                     PSEVIRQRPDVRSAEKQIDAQAAQLGATRADWWPKFYLTAKFGYASTDFQQLFMNNNM
                     YWQVAPAIQWTIFSGTQFSGSTQAAKANLEISINNYNNTILSALQDVDDAMKTYRYSV
                     NESEMRRKAFKEALTTFSLAMDLYKRGLINFQNVLDAQRSLLQYEDAVVSVEGNTYSA
                     LVNLFKALGGGWNNK"
     gene            2425..3558
                     /locus_tag="PK641_05450"
     CDS             2425..3558
                     /locus_tag="PK641_05450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPG55827.1"
                     /translation="MKNKSSIIIPAALIFLSMFSCANDQETKQVPLQKFSVANPEVRD
                     IVYRLEYPGYLQSERIVEIIARTEGFLIENKIKPGQKVSEGDVLFIIEPKTYEDKLTT
                     AEAALATAKSNLVFAQSSLDRIEEAAKSNAVSQIDLIQAQTNVDLCKSAIKTAEAQLS
                     IAQTQLGYCYIKAPCNGRVSKSTVDEGNYVKPQTQLTTLYKEDNMYAIFTIEASNLIW
                     AQKQAKSQGSINTKPFNTVDVIVADVTGETEIFQGELDYLSPSADLSTGTVDMRAVIK
                     NPKNELNNGVYVKISLPYKETKDAVLVPESSIGSDQSGRYIYVVKDDTVRYRNVKVGQ
                     LERDNMREITLGLQADELYITEALTRVRNGQAISPVLESTKQK"
     gene            3565..>3828
                     /locus_tag="PK641_05455"
     CDS             3565..>3828
                     /locus_tag="PK641_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPG55828.1"
                     /translation="MSKFFINRPIFAFVIALLIMLAGVITLINLPVALYPEITPPTVS
                     VTAAYPGANAQVISETVAAPIENKVNGVEGMLYMNSVSSSAGVY"
BASE COUNT         1352 a          574 c          694 g         1208 t
ORIGIN      
        1 tgatacagta gaagtgtcta ttgtgtatat gctattatta atgttattaa tatctatatt
       61 cataataaaa gcaattcaaa cagaacccat tgagttaccg gagcattgta agatttcttg
      121 gtcttacaag aaattccaat ggattattgt tgatgaacta gctgattgta gatataaaac
      181 tgcagaggcc gataaatttt catatttgtc atctaatgaa ttaaatgaga atgaaataaa
      241 tcgatataaa agcatattag atgaatggat gttgcaagat aaaccttatt taaataaaga
      301 tttcagagtt tctgatgtgc atgcaaaatt aaatattgat cgtcttaagt gtaatgaaat
      361 cttttcacgt gcgtataagt gctattttag gaattgggtt ttgcaatata gaatagaata
      421 tgccattaaa ttaatgaaag aaaatcctga taagcaggta aaagagattt catttgaggc
      481 cggttttact tctcaagcca tttttgcgcg atcgtttgca ctacaaatgg gaatgtcttg
      541 tactcaatac aaagagagat ttttgatata atttcaaaaa aactactatt tttttgtatg
      601 aaaagaagta ctttttatcg atttgtaaaa tgctgattaa ctgatacata agtatctgtt
      661 gtgtgggtgt tgacgtatag taaatgaatc tagtacaaat ttgcaaacta aaaatacaaa
      721 aaggcaaaat actttatttg caaaagataa tatgcctcaa tacatttgca ttgtcgaaag
      781 actaaaaatc tagtagcggc attttcaaaa ggaaggattt tgattaaaag gctgcgtatg
      841 attttaatta cttttgtatt gtttttttca tagtgatatt ttttcttcgt tttttagtga
      901 attagtgagt gaaaagtggc aggtaagcgt acctgccact ttttttttga ttatcccata
      961 ttttctttaa aaaaacctta cttttgcgat atataaataa aaacaaatgc aaaataaatt
     1021 aattctgata ctactcgtat ttttatccac atctttttct ttatatgcaa aaccaagtac
     1081 tgggcgtatt gattcgaata tcccgaaaaa ctggatttta aagtctgata cgtttttttt
     1141 agaacagcaa ccaatagaac aaaaatggtg gcaatctttt gatgatcccg ttttgaattc
     1201 aatcgaagaa ttggctattg ccaataatta tgacttagtt gcagcatcat atagaattgc
     1261 ccaagcaaaa gctaatatgc gtattgctga ggcagggttt tatcctcaaa tatcattagg
     1321 cgcagcttat gattataata aaaacattag cgcgatagga atgattgctc atacaggatc
     1381 tttaggtttg gattttcaat gggaaataga tataataggt tctgtaagaa ataaagcaaa
     1441 tgcacaaaag aaagtttatg aggcaacaca agaagagtat aatgcaatga tgactatttt
     1501 agtagcacaa gttgcagatg cttacttttc tttgcgtaca actcaactac gcttaaatgt
     1561 agtaaaacgt aatttagcta gtcagaaaga gacaatggat attacagaag cgcgtttcaa
     1621 ttcaggctta gtctctgcat tagacgtttc tcaagctaag tcgatatatt attctactgc
     1681 agcatcaaaa ccaaaattgg aatcattggt ttcaaagtac attaatacaa taggtgtttt
     1741 ggtcggaaaa ttaccatggg atttagaatc agatttagct ctagtaaaag atattccatt
     1801 gaaagagaat cctatattag tacccgttgg gataccatca gaagttattc gtcaacgtcc
     1861 cgacgtgcgt agtgcagaaa aacagattga tgcacaagct gctcaactag gagcaactcg
     1921 tgcagattgg tggcctaaat tttatctaac agccaagttt ggatatgcat caacagattt
     1981 tcagcaattg tttatgaata ataatatgta ttggcaagtt gcacctgcaa tccaatggac
     2041 tatattttca ggaacacaat tttctggaag cactcaagct gcaaaggcta atttagagat
     2101 ctctataaac aattataaca atacaattct ttcggctttg caagatgtgg atgatgctat
     2161 gaaaacatat cgttattctg tcaatgaatc tgaaatgcgt agaaaagcat ttaaggaggc
     2221 tctcacaact ttttcattgg caatggattt gtataaaaga ggtttgatta actttcaaaa
     2281 tgttttagat gcacaacgtt ctttgttgca atacgaagac gcagttgtat ctgtagaagg
     2341 caatacctat tcagctttag taaatctatt taaggcttta ggcggaggat ggaataataa
     2401 ataattaaat aaaaatcaca ctatatgaaa aataaatcaa gtataattat tcctgcagca
     2461 ttgatttttt tgtcaatgtt ttcgtgtgct aacgatcagg aaacgaaaca agtgcctcta
     2521 caaaaatttt cagtcgcaaa tcctgaggtt cgcgatattg tatatcgttt agagtatccc
     2581 ggttatttgc aatcagaaag aatagttgaa atcatagcaa gaacagaagg ttttttgata
     2641 gaaaataaga tcaaacccgg tcagaaagtt tcagaaggtg atgtcttatt tattattgag
     2701 cctaaaactt acgaagataa attaactaca gccgaagctg ctttagccac tgcaaaatca
     2761 aatcttgttt ttgcacaatc atctttagat cgtattgaag aagctgctaa aagtaacgcc
     2821 gtaagtcaga tagatttaat acaagctcaa actaatgtag acttgtgtaa gtcggcaatt
     2881 aaaacagctg aagcacaact tagtattgca caaacacagc ttggatattg ctatataaaa
     2941 gcaccttgca atggtcgtgt ttctaaaagt acagttgatg aaggcaatta tgtaaaacct
     3001 caaacacaat taactacctt atacaaggaa gataatatgt atgctatttt tacaatagag
     3061 gcaagtaatt tgatctgggc tcaaaagcaa gcaaaatctc aaggatcaat taatactaaa
     3121 ccttttaata ctgttgatgt tattgttgcc gatgtgaccg gtgaaacaga aatttttcaa
     3181 ggagaattag actatttatc tccatctgca gatttatcta ccggaacagt agatatgcgt
     3241 gcagttatta aaaatccaaa gaatgagtta aataatggag tatatgtaaa gatttcattg
     3301 ccatataagg aaacaaaaga tgcagtcctt gttcctgaaa gttcaatagg ctcagatcaa
     3361 tcaggaagat atatatatgt agtaaaggat gacaccgttc gttataggaa tgtcaaagta
     3421 gggcaattag aacgtgacaa tatgcgtgaa ataactttag gtttgcaagc agacgaatta
     3481 tatataacag aagcattgac tcgcgtaaga aatggtcaag caataagtcc tgtattagaa
     3541 tcaacaaaac aaaaataagc agccatgtcg aaatttttta tcaatcgacc gatatttgct
     3601 tttgttatag cactactaat tatgttggcc ggtgtaataa cactgataaa tttacccgta
     3661 gcactatatc ctgaaattac acctccaacg gtatctgtaa cagcagcata tcccggcgca
     3721 aacgcacaag ttatatccga aactgtagca gcaccaatag agaataaagt taatggtgtt
     3781 gaaggtatgt tatacatgaa ttcggtatca tcttcggcag gtgtatat
//
LOCUS       DAOPID010000151         3814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_32808_length_3814_cov_3.560255, whole genome shotgun sequence.
ACCESSION   DAOPID010000151 DAOPID010000000
VERSION     DAOPID010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32808_length_3814_cov_3.560255"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="PK641_05460"
     CDS             complement(<1..993)
                     /locus_tag="PK641_05460"
                     /inference="COORDINATES: protein motif:HMM:NF028048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starch-binding protein"
                     /protein_id="HPG55829.1"
                     /translation="MVLNTKLRLILTTLLLSLFVVSNAITVYYKDTNSWGGVNGHVWN
                     ASASSENTGFPGKQWILKCVDGGDTWYKYEYQSTSMNLKLTKFSDSNVGTGDIGPLTT
                     DCWIEGTSSTPHTDPITLANLESLTFTTSVAITFASDALAGWSTYLPTTTSDNENYSL
                     TYLYGTGSGIDVWYDTTKKKYVSTGEIIGYSASYIGQVVTFTWNITSETLSVTGFSIV
                     PDIPTPHYLGDIINLTAENGTGPTYTWYKSTDDGATYSVITGETTEMLTVFADRKTKY
                     KCVDDGTSGYTTYDIEPIIKCDPQNTTIEFTEDFGTVAQSDGGKGDGFWDKGRTT"
     gene            complement(1144..1911)
                     /locus_tag="PK641_05465"
     CDS             complement(1144..1911)
                     /locus_tag="PK641_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPG55830.1"
                     /translation="MNLSLKNPIIFFDLETTGIDIVKDRIVEISYLKVYPNGREETKT
                     IRVNPGMPIPKQATEVHHITDDDVKDCPSFKMVAKELARDFEGCDLAGYNSARFDLPL
                     LAEEFLRNEVDVDLSKRKMVDVQTIFHKMEQRNLSAAYKFYCNADLVGAHGAEADTKA
                     TYEVLKSQLDRYDNLQNDINFLSEFSSQNKNVDFAGRIVYNEKGEEIINFGKYKGRLV
                     VDVLKEDMGYYGWIMGADFPLSTKRVFTEIKLRNFNR"
     gene            complement(1923..3041)
                     /gene="dnaN"
                     /locus_tag="PK641_05470"
     CDS             complement(1923..3041)
                     /gene="dnaN"
                     /locus_tag="PK641_05470"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPG55831.1"
                     /translation="MKFVVSSSTLVEHLQVLSRVISNKNTLPIMNYFLFSLEGSKLTI
                     TASDQEITMLSTIEVENKGNDGRIAILPKYLLDILHELGDQPLAFEIDESNYAIVIKS
                     AKGKYSFIGHETEEYPAFRQLEGDVSSISIPCPLLLEGLSQTLFAVANDDLRPIMTGV
                     CFDITTDSLNLVSTDAHTLSRFKLNDIKSETNINFVLPQKAASLIKNVLSKETGDASM
                     KFDDKSIFCEISDYILVCRQIEGTYPKYNSVIPQDNPIKVIVDRLSLLSAIRRVSVCT
                     NQGSSLMRVSISNGNIYLSGQDIDFSVSGEENVSCQYDGEAIEIGFKAILLIDMLSNM
                     SASDIVIELSDKTRPGIFSPLNQAEGTEALMLLMPMMI"
     gene            complement(3111..>3814)
                     /locus_tag="PK641_05475"
     CDS             complement(3111..>3814)
                     /locus_tag="PK641_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55832.1"
                     /translation="KFEAAPWYYAPLLLPSAANVITKNTAPVRSDFTSIVELVGEEQT
                     ANIKKSILLASSNQTGVEKAPMPIRMDVVNLSPESPFFAYSYLPVAVLLEGSFKSVFA
                     NRMIPQDINMAGRKQLHSGNATKMIVVADGDIIRNDIQGFGSGFEALPLGYDRYMDKQ
                     FGNGTFVLNAINYLADDEGWMSLRGREWKLRLLDKQSIRTQKTYWQFMNMGLPIILLI
                     IFSFAYQLIRKKQYS"
BASE COUNT         1274 a          746 c          526 g         1268 t
ORIGIN      
        1 tgttgtgcgt cccttatccc aaaaaccatc acctttacca ccgtcacttt gtgcaacagt
       61 gccaaaatct tctgtaaatt caatggttgt attttgcgga tcgcatttga ttataggttc
      121 tatatcataa gtagtatacc ctgaagtccc atcatcaaca catttgtatt tggtttttct
      181 atctgcgaac actgttagca tttctgtagt ttcaccagtt ataacagaat acgtagcacc
      241 atcatctgta gatttatacc aagtatatgt aggtcctgtt ccattttcag cagtcaaatt
      301 aattatatcg ccaagataat gaggagttgg aatatcaggc acaatactaa agcctgtaac
      361 cgataatgtt tcactagtaa tattccatgt aaacgtaact acctgaccaa tatatgatgc
      421 actatatccg attatttctc cagttgacac gtacttcttt ttagtagtat cgtaccacac
      481 atctattccg gatccggttc cgtacaaata agtaagagaa tagttttcat tgtctgatgt
      541 tgttgtaggt aaataagtac tccacccggc caacgcatca gaagcgaatg ttatagcaac
      601 agatgttgta aatgtcagac tctctaaatt agccaatgta atgggatcag tatgtggagt
      661 tgaggaagtt ccttcaatcc aacagtcagt tgttaaagga ccaatatctc ctgtacctac
      721 atttgaatca gaaaatttag tcaatttcag attcatagat gtactttgat actcatattt
      781 ataccaagta tcaccaccgt caacacattt caaaatccat tgtttacccg ggaaaccagt
      841 attttcggag gaggcactag cattccaaac atgcccattt actccacccc aactatttgt
      901 atctttataa taaacagtaa tggcattact tacaacaaat aaggacaaca ataatgtagt
      961 caatattaat cttaacttag tatttaaaac catgatatat ttgattatgt atttgatttt
     1021 aaaaaatata gcttgctcga taattatacc gggcaagcta caaaattata actttttatg
     1081 aattatgtgt gttttttttt actttattga atgctaaaaa caaaaatcat ctttatgcaa
     1141 acgttatctg ttaaaattgc gcaatttaat ttctgtaaaa actcgttttg ttgacaaagg
     1201 aaaatcagct cccattatcc aaccataata tcccatgtct tcttttaaaa catcaacaac
     1261 caaacgaccc ttatattttc caaaattaat tatctcctca cctttttcat tataaacgat
     1321 tcttcctgca aagtcaacat ttttattttg agatgaaaat tcggacaaga aattaatatc
     1381 attttgaaga ttatcatagc gatccaattg cgatttaagc acctcataag tagctttagt
     1441 atcggcttct gcaccatgtg ctcccaccaa atctgcatta caataaaact tatatgcagc
     1501 agacaaatta cgttgctcca ttttatgaaa tatagtttga acatctacca ttttacgttt
     1561 agacagatca acatctactt catttctcaa aaattcctcg gctaaaagag gtaaatcaaa
     1621 acgcgccgaa ttataaccgg ccaaatcaca tccttcaaaa tctctggcca actcttttgc
     1681 taccatctta aagcttggac aatcttttac atcatcatct gtaatatggt gaacttctgt
     1741 agcctgctta ggtattggca taccgggatt gacacgaata gttttagttt cttctcttcc
     1801 gttaggatac acctttaaat acgatatttc tactattcta tcttttacta tatcaatacc
     1861 tgtagtttcc agatcaaaaa aaataatagg atttttaaga cttaaattca tctttatcaa
     1921 tattatatca tcattggcat caataacatc aaagcctctg ttccttctgc ttgatttagc
     1981 gggctaaata taccaggacg tgttttatca gataattcga ttacaatatc tgaagctgac
     2041 atatttgaca acatatcaat tagcaaaata gccttaaaac ctatttcaat agcttcgcca
     2101 tcatactgac atgatacatt ttcttctcct gatacagaaa aatcaatatc ttgacccgat
     2161 aaataaatat ttccgttact gatagatacc ctcatcaaac tactaccttg atttgtgcaa
     2221 acagatacac gacgaatcgc tgacaaaaga gataatctat caacaataac cttaattgga
     2281 ttgtcttgag ggataaccga attatattta ggatatgttc cttctatttg tctacaaacc
     2341 aaaatataat ctgatatttc acaaaatata ctcttgtcat caaatttcat tgaagcatct
     2401 cctgtctcct ttgataagac atttttaatc aaagatgctg ctttttgtgg caacacaaaa
     2461 tttatattag tttcagactt tatatcattt agtttaaaac gtgacaatgt gtgagcatct
     2521 gtactgacta aattcaagct atcagttgta atatcaaagc aaacaccagt cattattgga
     2581 cgcaaatcat cattagcaac tgcaaacaat gtttgagata atccttccaa taaaagtgga
     2641 catggtattg atattgaact tacatcccct tctaattgac gaaatgcagg atattcttca
     2701 gtttcgtgtc ctataaaact atacttacct tttgcactct taataacaat agcataatta
     2761 ctttcgtcta tttcgaatgc caatggttga tcgcccaatt cgtgtaaaat atctaataaa
     2821 tattttggta aaattgctat gcgaccatca ttacccttgt tttcaacttc aatagtagaa
     2881 agcatcgtaa tctcttgatc tgaagctgta atagttagtt ttgatccctc taatgaaaac
     2941 aaaaaatagt tcattatagg taatgtgttt ttattactga taacacgtga aagtacctgc
     3001 aaatgctcca ctaaagtgga cgaagaaaca acgaatttca tagaattaaa tttattaatg
     3061 atagctacaa agttatgatt attttttaat atttaaaact caaaacacta ttaagaatat
     3121 tgttttttgc gaattaactg ataagcaaaa gaaaaaatta ttaataagat tataggtaat
     3181 cccatattca taaattgcca atatgttttt tgagttctaa tactttgctt atcaagtaaa
     3241 cgcaatttcc attctctacc cctcaaagac atccagccct catcatcagc caagtaatta
     3301 attgcattaa gcacaaatgt accattaccg aattgtttat ccatataacg atcataacct
     3361 aatggtaatg cttcaaaacc cgaaccaaag ccttgaatat cattcctaat tatatctccg
     3421 tcagctacaa caatcatctt tgtagcattt cctgaatgca actgtttgcg acctgccata
     3481 ttgatatctt gcggtatcat acgattggca aatacagact taaaagaacc ttccaataaa
     3541 acagccacag gcaaataaga atatgcaaaa aatggagatt ccggagacaa atttactaca
     3601 tccatgcgaa taggcattgg tgccttttca acgcccgtct gattagaact tgccaacaaa
     3661 attgactttt taatatttgc agtttgttct tctcctacca gttcaactat tgatgtaaag
     3721 tcagatctca caggagcagt gttttttgta ataacattcg ctgctgatgg taataacaat
     3781 ggtgcataat accatggagc tgcctcaaat ttag
//
LOCUS       DAOPID010000152         3778 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_33241_length_3778_cov_2.745098, whole genome shotgun sequence.
ACCESSION   DAOPID010000152 DAOPID010000000
VERSION     DAOPID010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33241_length_3778_cov_2.745098"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PK641_05480"
     CDS             <1..411
                     /locus_tag="PK641_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55833.1"
                     /translation="IGNRYNISNKLIDTLIVISLCCLIILSIYCCNLDFARAKQYYND
                     VNLRNEKLIQLENQGFKGIAIVKPFKIKQELSSYAKLRNAMVKYIKPSAMIEEYYFPY
                     MLYGISEGKTSWKNNAIKQYFSLNYDIIGWENCE"
     gene            443..1294
                     /locus_tag="PK641_05485"
     CDS             443..1294
                     /locus_tag="PK641_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007845031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG55834.1"
                     /translation="MTSDKTWTTIIKPHNKLWEVDFKELWRYRDLFMMYVKRDIITMY
                     KQTILGPLWFVIQPAITTIMYMVVFGGIAGISTDGLPQPLFYLAGICLWQYFADCLNK
                     TSSTFISNQGIFGKVYFPRLVAPLSTIVSGLVKLGIQILLFVIVYIYFLAIGTPVEPN
                     WALSLFPLLVIMIAGLALGFGIIISSLTTKYRDLTILFTFIVQLWMYGTPVIYPLSSI
                     PEGKIKLLMQLNPITPIVETFKYGTLGIGEFSWTMLGYSFCFMLVVLAIGIILFNKVQ
                     RSFMDTV"
     gene            1528..1893
                     /locus_tag="PK641_05490"
     CDS             1528..1893
                     /locus_tag="PK641_05490"
                     /inference="COORDINATES: protein motif:HMM:NF008911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPG55835.1"
                     /translation="MQDYHKLTVWTKSLDLVKDIYQITTAFPKTESYGLSNQIQRAAV
                     SIPSNIAEGSSRSSKNDYIRFLEIALGSAYEVETQLRISKELNYISENTFNSILDKID
                     HIQRQLVQFIKRIRETLNA"
     gene            1948..3237
                     /locus_tag="PK641_05495"
     CDS             1948..3237
                     /locus_tag="PK641_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008652411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide ABC transporter ATP-binding
                     protein"
                     /protein_id="HPG55836.1"
                     /translation="MTAIEFNNISKQYRLGLVSTRTLSNDLNRWWTMNILHKEDPYLK
                     IGSVNDRSQKADSDYVWALKNIDFKVEQGDVIGIIGKNGAGKSTLLKLLSRVTMPTTG
                     TIKARGRIASLLEVGTGFHPELTGRENIYMNGAILGMSKSEITQKLDEIIDFAGVERY
                     IDTPTKRYSSGMTVRLGFAIAAHLDPEILVVDEVLAVGDAEFQKKAIGKMQEISKREG
                     RTVLFVSHNMSAIRNLCKSGVVLENGYVSFSGTAEESIAKYLSINQSTNLLSISQRKD
                     RIGNGKIRIKNILLKNKNGVVVEDVFVGSYLKIEFHFDKEESVDLSQMIVACSLSNNY
                     GDVVVLWVSDEMENCFHKIKDNIFSLEIPEINMRPNIYNMNFQISLKTTSQSDFCDVM
                     NSAISISVLNNSFYQKGIDLRTSRGYIPLLKAKYIID"
     gene            3242..3649
                     /locus_tag="PK641_05500"
     CDS             3242..3649
                     /locus_tag="PK641_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HPG55837.1"
                     /translation="MGTLEDKVIIIPRHLIRDDRGWFLKTLNGKEENLSQNVGEVYFT
                     SAKQGKSKGAHYHIMAKEWFTLISGKAILKLEDIYSHDFLSISLESEHPITVYIPPYV
                     AHEVVNNGSEDFILCAYTDLQYNSIDTIPYNLK"
     gene            3668..>3778
                     /locus_tag="PK641_05505"
     CDS             3668..>3778
                     /locus_tag="PK641_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPG55838.1"
                     /translation="MKAVILAGGFGSRLSEATNLIPKPMVEIGGKPILWHI"
BASE COUNT         1344 a          509 c          683 g         1242 t
ORIGIN      
        1 ataggtaata gatataatat atcaaataaa ttgattgata cattgattgt gatctcatta
       61 tgttgtttga taatactgtc tatatattgt tgcaatttgg attttgccag agcaaaacaa
      121 tattacaatg acgtcaattt aagaaacgaa aaattaattc aattagaaaa ccaaggattt
      181 aaaggtatag ctattgtaaa accatttaaa attaaacagg aattatcatc atacgctaaa
      241 cttagaaatg ctatggttaa atatattaaa ccttcagcaa tgattgagga atattatttc
      301 ccatatatgc tatatggaat aagtgaaggt aaaactagtt ggaaaaataa tgcaattaaa
      361 cagtattttt ctttgaatta tgatattatt ggttgggaaa attgtgaata atctttatgc
      421 tttttgaaaa taaataattt atatgacctc agataaaact tggacaacta ttattaaacc
      481 tcataataaa ttatgggagg ttgattttaa agaattgtgg agataccgcg atttgtttat
      541 gatgtatgtt aaaagagaca tcataacgat gtataagcaa accattcttg gtccgttatg
      601 gttcgttata caaccggcaa taacaacaat tatgtatatg gtagtgttcg gcggcatagc
      661 cggcattagt actgatgggc taccacagcc tttgttttac ttagcaggta tttgtctttg
      721 gcaatatttc gccgactgtt taaataaaac atcttctaca tttattagca atcaaggtat
      781 ttttggaaaa gtatattttc ctcgtttagt agcaccatta tcaacaatag tatcaggctt
      841 agttaaatta ggaatacaaa ttttgctgtt cgtaattgta tatatatatt ttcttgcaat
      901 aggcacgcct gttgagccta attgggcatt gagtttgttt cccttacttg taataatgat
      961 agccggctta gcattaggtt ttggtattat catatcatct cttaccacta agtatagaga
     1021 tttaactatt ctatttacat ttattgtgca attatggatg tacggcacac ctgtaattta
     1081 tcccttaagt tctatccccg aaggcaaaat taaattgcta atgcaactta atccgataac
     1141 tcctatagtt gaaacattta aatacggaac acttggaatt ggtgaattct cgtggactat
     1201 gttaggatat agtttttgct ttatgttagt agtactcgca ataggtatta tactatttaa
     1261 caaagtacaa agaagtttta tggatacagt ttaattttct gctcgaagca gaaaattaaa
     1321 cgtatctcgt atctcgttaa gagttactcg ggaaatagaa gacgcgggaa aacgttactc
     1381 gttgaacgtt aagcgtaaag cgaaagaaaa cgttactcgt tgaacgttaa gcgtaaagcg
     1441 aaaggaaacg ttactcgttg aacgttaagc gtatctagta aatcgtcaag cggaaaacaa
     1501 aaatcgtttt tttttagaga atttaatatg caggattatc ataaattgac agtttggaca
     1561 aaatcattgg atttggtaaa agatatttat caaataacta ctgcatttcc caaaactgag
     1621 tcctatggat tgtcgaatca aatacaacga gcagctgtat ctattccatc aaatattgca
     1681 gaaggtagct ctcgctcctc aaaaaatgat tatatacgtt ttttagaaat tgcattaggt
     1741 tctgcctatg aggtagaaac acaattacgc atatcaaaag aacttaatta tatttcagaa
     1801 aatacattca attcaatttt agataaaatt gatcatatac aaagacaact agtacaattt
     1861 attaaaagaa ttcgagaaac gttaaacgct taactaacaa cgcttaacta acaacgctta
     1921 actaacaacg cttaacgaat tacgcttatg acagccatag aatttaacaa tatcagtaaa
     1981 caataccgcc tcggattggt aagtacacgc actttaagca atgaccttaa ccgttggtgg
     2041 acaatgaata ttttgcataa agaagatccg tatcttaaga ttggttctgt aaacgatcgt
     2101 tcacaaaagg ctgatagcga ctatgtgtgg gcattaaaga atattgattt taaagtagag
     2161 caaggtgatg tgataggtat aattggtaaa aacggagccg gtaaaagtac attgttaaaa
     2221 ttattgtcaa gagttacaat gcctacaacg ggtactatta aagcacgtgg ccgaatagca
     2281 tcattacttg aagttggtac cggattccat cctgaactta cagggcgtga aaatatctat
     2341 atgaatgggg ctattttagg tatgagcaag tctgaaatta cacaaaaatt agatgaaatt
     2401 atagattttg ccggtgttga acgttatatt gatactccaa caaaacgtta ctcaagtggt
     2461 atgacagtac gtttgggctt tgccattgcg gcacatttgg accctgaaat tctagttgtt
     2521 gacgaagttt tagcagtagg agatgctgaa tttcagaaaa aagctatagg taaaatgcaa
     2581 gaaatttcaa aaagggaagg aagaactgta ttgtttgtga gtcataatat gtcagctatt
     2641 cgaaatttat gtaaatccgg agtagttttg gagaatgggt atgtgagctt ttcaggaaca
     2701 gcagaggaat ctattgcgaa atatttatca ataaatcaaa gtacaaatct tctttctata
     2761 tctcaaagga aagatagaat tggaaatgga aaaatcagga taaaaaatat tttattaaaa
     2821 aataaaaatg gtgttgttgt tgaagatgtg tttgtaggtt cttatttgaa aattgaattc
     2881 cattttgata aggaggaaag cgttgactta tctcaaatga ttgttgcttg tagtttatca
     2941 aataattatg gggatgttgt tgttttgtgg gtttcagatg aaatggaaaa ttgttttcat
     3001 aaaataaaag ataatatttt ttcattggaa attcctgaaa taaatatgag acctaatatc
     3061 tataacatga attttcaaat ctctctaaaa accacgtctc aaagtgattt ttgtgatgtt
     3121 atgaatagtg caatttctat ttcagtttta aataattcat tctatcaaaa aggaattgat
     3181 ttaagaacaa gtcgtggata tataccatta ttaaaagcta aatatattat agattaattt
     3241 tatgggaact ttagaagaca aagtgataat aatacctagg catttgatta gagatgatag
     3301 agggtggttc ttaaaaacat taaatggcaa agaagaaaat ttatcacaaa atgttggaga
     3361 agtttatttt acctcagcca agcaaggtaa atcaaaaggg gctcattatc atattatggc
     3421 aaaagaatgg tttactctta tttcaggaaa agcaatttta aaactagaag acatatattc
     3481 tcatgatttt ttgtcaattt ctttagagtc ggaacatcca ataacggtat atattcctcc
     3541 atatgttgct catgaagtag taaacaatgg tagtgaagat tttattttgt gtgcatatac
     3601 tgatttacaa tacaattcta tagatacaat tccatataat ctaaaatagt ttaaataaat
     3661 taataggatg aaagcagtaa ttttagcagg cggatttggt tctcgattaa gtgaagctac
     3721 taatttgata cccaaaccga tggttgaaat cggagggaag ccgatattgt ggcatatc
//
LOCUS       DAOPID010000153         3773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_33288_length_3773_cov_4.570199, whole genome shotgun sequence.
ACCESSION   DAOPID010000153 DAOPID010000000
VERSION     DAOPID010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33288_length_3773_cov_4.570199"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..1283)
                     /gene="mraY"
                     /locus_tag="PK641_05510"
     CDS             complement(57..1283)
                     /gene="mraY"
                     /locus_tag="PK641_05510"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPG55839.1"
                     /translation="MFYYLFRFLENAFDIPGAGLFSYVSFRAAMTFITSVFISMIIGK
                     DIIKYLQKKQIGESIRNLGLEGQLSKKGTPTMGGIIIIIAILVPCLLFGRLRNIYLLL
                     MLVSTAWLGVFGFLDDYIKVFKKNKEGMPGKTKILGQIGLGLIVGFTLFLSPEAVTVE
                     KVGKAQSYEIESAELNTGDKAVKSTRTTIPFVKSNDLDYADLLSFLGENAQKGAWILF
                     VLITIFVITATSNGANLTDGLDGLAAGTSAIIGVTLAILAYISSNVGFAGYLNVMFIP
                     GSEELVVFAAAFIGALVGFLWYNSYPAQVFMGDTGSLTIGGIIAVFCIIIHKELLLPI
                     LCGIFLIEDLSVMLQVSYFKHTKRKYGEGKRIFLMSPLHHHFQKKGIPEAKIVTRFWI
                     IGIALAIITILTLKIR"
     gene            complement(1300..2757)
                     /locus_tag="PK641_05515"
     CDS             complement(1300..2757)
                     /locus_tag="PK641_05515"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HPG55840.1"
                     /translation="MTVSELTKNLKISKHFGNLNLEVSGLSFDSRKTETGHLFIAIKG
                     TTSDGHDYIETAINNGATAIICEQLPKEINSKICYVQTADSSDALGKVAAVFFDLPSE
                     KMIVVGVTGTNGKTTVASLLYYMFKKLGYKVGLLSTVCNYVDEEAVPSTHTTPDPIEM
                     QSLMARMAKKSCQYVFMEVSSHAINQKRISGICFDGGIFTNLTRDHLDYHKTMDEYLK
                     AKKCFFDNLPSHAFAITNLDDKNGKVMLQNTQANKYAYALQTSADFKGKVIEESFEGM
                     TININNKEVSTHFVGRFNASNLLAVYGAAYLLRIPSDDILLVLSTLLPVNGRFDSVRS
                     NDGRNAIVDYAHTPDALENVIETINQIRRNSQQKNTLITVVGCGGNRDKGKRPIMAKE
                     AVKGSDKVIITTDNPRFEEPSDIIKDMLDGLDETQKAQVLVIEDRKQAIKTACMLAKQ
                     GDVILVAGKGHENYQEIKGVKHHFDDKEIVSECFK"
     gene            complement(2757..>3773)
                     /locus_tag="PK641_05520"
     CDS             complement(2757..>3773)
                     /locus_tag="PK641_05520"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPG55841.1"
                     /translation="ANRIMKDWNFHSGNGGFGVISVPTVLHQSSNVGVSLLIDNNYRN
                     NPQRFVDGIHRTKFDSPIDLELPGHGKVVIKDTDNKTWSNTTLPWMSIGYEVLVPPIY
                     TLMLYNAIANDGKMMKPKFTYGLSQNGSVVEEFEPEVINESICSKETLGKVKTMLEGV
                     VSKGTAKSVQSKLFTTAGKTGTAQIFLQGTNKNAEGRTRHQITFCGYFPADKPMYSCI
                     VYIREPQGYASAGGMCGKVFKELAEKAFILGGGDLPIWAKDSSNVDMTIDKAIVKEKI
                     KPITTDDNIMPDVMGLTASDAIYILENKNVRVFLNGYGHVTSQSIPTGTEIKKGMTVI
                     LTLN"
BASE COUNT         1093 a          740 c          626 g         1314 t
ORIGIN      
        1 gctgctcccg taccgctctc accacctcct aatacgatga ttttttttgt catagcctat
       61 cggattttta aagttaatat tgttataatt gccaacgcaa tgcctataat ccaaaatctt
      121 gttacaatct ttgcctcagg aatgcctttc ttttggaaat gatggtgtag cggagacatt
      181 agaaatattc gtttaccttc gccatatttg cgttttgtat gtttaaaata tgatacttgt
      241 agcataacag ataggtcttc aattaagaag ataccgcata aaataggcaa aagcaactct
      301 ttgtgtatga ttatgcagaa cactgcaatg atacctccaa ttgttaaact tccggtatct
      361 cccataaata cttgagcagg ataggagttg taccataaga aacctactaa tgcacctata
      421 aatgcggctg cgaaaactac taactcttcg cttcccggta tgaacataac atttaggtaa
      481 cctgcaaatc cgacattgct ggatatgtat gcaagaatag ccagtgtaac tccgattatg
      541 gctgatgttc cggctgctaa tccatctaga ccatcagtta gatttgctcc attagatgta
      601 gctgttatta cgaatattgt aattaataca aatagtatcc acgctccttt ttgtgcgttt
      661 tctcccaaga atgataggag gtctgcataa tctaaatcgt tgctttttac aaatggaata
      721 gttgttctgg ttgattttac ggctttgtcg ccggtgttaa gttcggctga ttcaatctca
      781 taagattgtg ctttgcccac tttttcaaca gtaacagctt ccggactcaa aaacaaagta
      841 aagccaacta taagaccaag tccaatctga ccaagaattt tagtcttgcc cggcattcct
      901 tctttattct ttttaaatac cttgatgtaa tcatctaaaa atccaaatac tccaagccat
      961 gcagttgaaa ccagcatcaa taaaagatat atatttctaa gtctgccaaa caaaagacat
     1021 ggtacaagaa tcgcaattat aattattata ccacccattg taggtgtgcc ttttttactt
     1081 aattgacctt ccaaccctaa attcctaatc gattcaccaa tctgcttttt ttgcaaatac
     1141 tttataatgt ctttacctat aatcatagat ataaaaacag atgtaataaa ggtcatagct
     1201 gccctaaaag atacgtagct gaacaaaccg gctcccggta tgtcaaacgc attttctaaa
     1261 aatctaaaca gatagtaaaa catagtatta gcttattaat tatttaaagc attctgaaac
     1321 aatttctttg tcatcaaaat gatgtttgac tcctttaatt tcttgatagt tttcgtggcc
     1381 tttacctgct acaagaataa catctccttg ttttgcaagc atgcaagccg tttttattgc
     1441 ttgtttacgg tcttcaataa ctaaaacttg tgctttttga gtttcatcta atccgtcaag
     1501 catatcttta ataatgtctg aaggctcttc gaatcgaggg ttgtctgttg tgataataac
     1561 tttatcgctg ccctttacag cttcttttgc cattattgga cgtttaccct tatctctatt
     1621 tccaccgcaa cccacaacag ttatcagtgt gtttttttgt tgactatttc tgcggatttg
     1681 attaattgtt tctattacat tttctaaagc atcgggagtg tgtgcatagt caacaattgc
     1741 attacgtcca tcatttgaac gaacactgtc gaatctgcca ttaaccggca aaagtgtact
     1801 taaaacaagc aagatatcat ctgaaggaat tcttaataga taagcagctc cgtatacggc
     1861 cagcaagttt gaagcattga atcttcctac aaagtgagtt gaaacttctt tgttgttgat
     1921 gttgattgtc ataccctcaa aactttcttc tatcactttg cctttaaaat cagctgatgt
     1981 ttgaagtgca taagcatatt tatttgcttg agtattttgg agcattactt ttccattctt
     2041 gtcgtccaag ttagttatgg caaaagcatg agaaggaaga ttgtcaaaaa agcatttttt
     2101 tgcctttaga tattcatcca tagttttatg ataatcaaga tgatcgcgag ttagatttgt
     2161 gaagatacca ccatcaaagc atatacctga tatgcgtttt tggtttatgg catgcgagct
     2221 tacttccatg aaaacatatt ggcaagattt ttttgccata cgagccataa gagattgcat
     2281 ctcaataggg tcaggcgttg tgtgtgtact tggaacagcc tcttcatcta cataattgca
     2341 tactgttgat aacaaaccga ctttgtagcc gagtttttta aacatgtaat acagaaggct
     2401 agctacagtc gttttgccat tggtgccggt aactcctaca actatcattt tttctgatgg
     2461 caaatcgaag aatacggcag ctactttacc aagtgcatca gaactatcag ccgtttgaac
     2521 gtagcaaatt ttagaattaa tctctttggg taattgttca caaattatag ctgtagcacc
     2581 attattaatg gcagtctcga tatagtcgtg accatctgaa gtagtacctt ttatagcaat
     2641 aaacaaatgt cccgtctctg ttttgcgaga atcaaaactt aaacctgaaa cttcaaggtt
     2701 taaattgccg aagtgcttac tgattttcag attctttgtt aattcgctga ctgtcattaa
     2761 tttaatgtta atattactgt cattcctttt tttatttctg tacctgtagg tatggattgt
     2821 gatgtaacgt gcccgtatcc atttaaaaaa acacgtacat ttttgttctc taaaatataa
     2881 atagcatctg aagctgttaa tcccattaca tcaggcatta tattatcatc ggtagtgatg
     2941 ggtttgattt tttctttaac aattgcctta tctattgtca tatcaacatt tgaactatct
     3001 ttggcccata ttggcagatc tccaccgcct aatataaatg ccttttcagc caactcttta
     3061 aatactttcc cacacatgcc tccggcagaa gcgtatcctt gaggttctct tatgtaaaca
     3121 atgcatgagt acataggctt gtctgcgggg aaatatccgc aaaatgttat ttgatgacgt
     3181 gtacgtcctt ctgcgttttt gtttgtgcct tgtaaaaata tttgagctgt acctgtttta
     3241 ccggctgttg taaaaagttt tgattgtaca gattttgcag tacccttgct tacaactcct
     3301 tctaacatgg tttttacttt ccctaatgtt tcttttgaac aaattgattc gtttataact
     3361 tcaggttcaa attcttcaac tacactacca ttttgactta aaccatatgt aaattttggc
     3421 ttcatcattt taccgtcatt tgctattgca ttatacagca tcagtgtata tatgggtgga
     3481 accagaacct catatcctat tgacatccaa ggcagtgttg tattcgacca agttttgtta
     3541 tcagtatcct taataacaac ttttccatgt cccggaagtt caaggtcgat aggagagtca
     3601 aacttagtgc ggtgtatgcc atcaacaaaa cgttgtggat tatttctgta attattgtcg
     3661 attagtaatg atacacctac gtttgatgat tgatgaagta cagttggaac acttattaca
     3721 ccaaatcctc cattcccgct atgaaagttc caatctttca taattctgtt tgc
//
LOCUS       DAOPID010000154         3769 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_33336_length_3769_cov_4.578891, whole genome shotgun sequence.
ACCESSION   DAOPID010000154 DAOPID010000000
VERSION     DAOPID010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33336_length_3769_cov_4.578891"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="PK641_05525"
     CDS             <1..438
                     /locus_tag="PK641_05525"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPG55842.1"
                     /translation="VFEHQIDVAIQKSLPIVIHCRDSFAEVIASLRKFDKSKLRGIFH
                     SFSGVKDQAREIFLSGDFYLGIGGVCTYKNAKFSERLFEIPIDRIVLETDAPYLTPVP
                     FRGKRNEPAYLVYVAKKLASVYKIEEYELAEQTSKNAENLFLM"
     gene            681..767
                     /locus_tag="PK641_05530"
     tRNA            681..767
                     /locus_tag="PK641_05530"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:715..717,aa:Ser,seq:gga)
     gene            883..1680
                     /locus_tag="PK641_05535"
     CDS             883..1680
                     /locus_tag="PK641_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPG55843.1"
                     /translation="MKKLFAVLAFIASVSLMSLYAQDAEQSTDSAATEQVATVSEADA
                     PAATIDEAEETGIHQAIKQKFIEGGAGFMSTVALCLIFGLAVAIERIIYLSMATADSK
                     KLLASVDKALSEQGVEAAKDICRNTRGPVASIFYQGLMRIDEGIDVAEKSIVAYGGVQ
                     AGLLERNLSWIGLFIGLAPMLGFLGTVVGMIEAFDRIQQAGDISATIVAGGIKVALIT
                     TVGGLIVAIILQLFYNYILSRIDAIVGDMEDSSISMLDILMKKELKK"
     gene            1700..2155
                     /locus_tag="PK641_05540"
     CDS             1700..2155
                     /locus_tag="PK641_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55844.1"
                     /translation="MQNKLSTITLYVLFVATVIVSALFFFGGNVDSSAEYVEPKFTQE
                     LIYLMYAFVAIAAIIVFGFQIWQLLLKLKSNPKQAVKSVAGVVILFILLLVLYLFSSG
                     EPIKILGMEDLELSSTMWKLVDMQLYTMYILTLVGLLLVLVGGFAKKIK"
     gene            2171..2761
                     /locus_tag="PK641_05545"
     CDS             2171..2761
                     /locus_tag="PK641_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPG55845.1"
                     /translation="MSKKKRKVPEINSSSTADIAFLLLIFFLVTTTMAVNKGLARRLP
                     PPVPADQKTEDLKVKERNVFVVLINSDNQLLVQNEYMDISKLKTKAKEFIVNNDDDPN
                     MPEKSVVNIDYFGNVMVTKDHVISLQNDRGTSYSKYIEVQNELVAAYNELRNDLARTK
                     WGVSYEELDESKQNAVQKYFPQKISEAEPKNYGGNK"
     gene            2761..3237
                     /locus_tag="PK641_05550"
     CDS             2761..3237
                     /locus_tag="PK641_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPG55846.1"
                     /translation="MGRFRDSSSHEMPAISTASLPDIIFMLLFFFMISTSMKEVDYRV
                     GIRPPEATELVSLEKKSLVRFIYVGTPVQKYRSQYGTESRIQLDDAFAEVSQIENYVV
                     AERSAMPESDQDLMTVSLKIDKECKMGIVTDIKQALRRAYALKINYSAIPRANSQF"
     gene            3339..>3769
                     /locus_tag="PK641_05555"
     CDS             3339..>3769
                     /locus_tag="PK641_05555"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPG55847.1"
                     /translation="MYLLLSFLFTFTFPFDSFSEKPDLIQPMIIPVELSANFAELRPN
                     HYHSGVDFKTMQIIGQNVYSVDDGYVARINISGSGYGNALYINHPKSGLTSVYAHLDN
                     FESFIDSIIKLKQYADESFEVDFYLSPDQLPVSKGQRVAKSG"
BASE COUNT         1191 a          542 c          738 g         1298 t
ORIGIN      
        1 gtttttgaac atcagataga tgtcgctata caaaaatcac ttccgattgt tatacattgt
       61 cgcgattctt tcgcagaggt aatagctagt ctgagaaaat ttgacaaatc aaaattaaga
      121 ggaatttttc atagtttttc cggagtaaaa gatcaagcga gagagatttt tttgtccggc
      181 gatttttatt tgggtatagg tggagtatgt acttacaaaa atgcaaaatt ttcggaacgt
      241 ttgttcgaaa taccgattga tcgtattgta ttggagaccg atgccccata tttaacacct
      301 gtgccatttc gtggtaaacg taacgaaccg gcctatttgg tatacgtggc taaaaagttg
      361 gcttctgtat acaaaattga ggaatatgaa ttagcagaac aaacatctaa aaatgcagag
      421 aatctatttc ttatgtagtt tttattatga tgtattttgc atctaattaa tcgattatga
      481 tacgatatta atgtaatatc aatattatga tgatgtatta ttttatgatt ataatttcaa
      541 tttatcacta ataattgctt tgttattatt attttacatt acattgaaaa aaattgttca
      601 aaaattattg taggttcgta tactttttgt aacttgcagc cgcttttgta ggctctcgta
      661 tcaagggctt gaaagacatt ggagagatgc tcgagcggtt gatgaggcac gcctggaaag
      721 cgtgtatact ggaaacagta tcgcgggttc gaatcccgct ttctccgcat tatgcgaagt
      781 ttagcggtca ttattgataa acaatttgtg ttagtgttac aagataaaat cttttaaaac
      841 aatcattaat aaaaatatta ttttaatttt aaacaaaaag acatgaaaaa gttatttgct
      901 gtgttagcgt ttatcgctag tgtaagttta atgagtttat atgctcaaga tgcagaacaa
      961 tcaacagatt ctgctgcaac agagcaagtt gctactgttt ctgaggctga tgctccggct
     1021 gcaactatag atgaagctga agaaacaggt attcatcaag caatcaaaca aaaatttatt
     1081 gaaggtggtg caggttttat gagtactgtt gcattgtgtc ttattttcgg tttggcagta
     1141 gctattgagc gtataatata tttgagcatg gctacagctg attctaaaaa attattggct
     1201 agtgtagata aagctttgtc tgaacaaggt gttgaagctg caaaagatat ttgccgcaat
     1261 acccgtggtc ctgttgcttc tattttctac caaggtttaa tgcgtattga tgaaggaatt
     1321 gatgttgctg aaaaatcaat agttgcttat ggtggggtac aagccggtct tttagagaga
     1381 aacttaagtt ggataggttt atttattggt ttggctccta tgttgggatt cttgggaact
     1441 gttgtaggta tgattgaggc atttgaccgc atccaacaag ctggtgatat ttctgctact
     1501 attgttgccg gtggtatcaa ggttgctctt atcacaacag taggtggttt gatcgttgct
     1561 attatcctac aattatttta caattacatt ttatcacgta ttgatgctat tgttggtgat
     1621 atggaggatt cttctatcag tatgttagat atcttgatga aaaaagaatt gaaaaaataa
     1681 ttaataggag ataataatta tgcaaaataa actatcaaca ataacattat acgtgctctt
     1741 tgtagcaacg gtaattgtat ctgccttatt cttttttggc ggcaacgttg attcttcagc
     1801 cgaatatgtt gagccaaaat ttacacaaga acttatatat cttatgtatg catttgttgc
     1861 aatagcagct attatagttt ttggatttca aatatggcaa ttattactaa aactgaagag
     1921 taatcctaaa caggctgtta aatcagttgc aggtgttgta attctgttca tattactttt
     1981 agttctttat ttattttcaa gtggtgagcc tattaaaatc ttaggtatgg aagatcttga
     2041 attaagctct actatgtgga aattggttga tatgcaattg tatacaatgt atatcttaac
     2101 tttagtaggt ctattattgg ttttggttgg cggtttcgca aaaaaaataa aataaaaaac
     2161 tatcaggatt atgagcaaga aaaaaagaaa agtaccggag attaattcaa gctccacagc
     2221 cgacatagcc tttttgcttt tgatattctt tctcgtaacc accactatgg ctgtaaataa
     2281 aggattggca cgtcgtttgc cacctccggt tcccgctgat caaaaaacgg aagacttgaa
     2341 ggtaaaagaa cgtaatgtgt tcgtcgtttt aattaacagc gataaccaat tgttggtgca
     2401 aaacgagtat atggatatat ctaagttgaa aactaaagcc aaagagttta ttgttaataa
     2461 tgacgatgat cctaatatgc ctgaaaaatc tgttgtgaat attgactatt tcgggaatgt
     2521 aatggtgaca aaagatcatg ttatatcatt gcaaaacgac cgtggtactt cgtattcaaa
     2581 atatattgag gttcaaaacg aattagtagc agcttataat gaattgcgta atgatttggc
     2641 tagaactaaa tggggtgtat cttatgaaga attagatgaa tcaaaacaaa atgcagttca
     2701 gaaatatttt ccacaaaaga tttcggaggc agaacctaaa aattatggag gaaacaaata
     2761 atgggacgat ttagagattc ttcttcgcac gaaatgcctg cgattagtac ggcgtcttta
     2821 cctgatatta tttttatgtt attgttcttc tttatgattt caacatcaat gaaagaagta
     2881 gactataggg ttggtattcg tcctcctgaa gcaacagaat tagtatctct ggaaaagaaa
     2941 agtttggttc gttttatata tgtaggaact cctgttcaaa agtatcgttc tcaatatggt
     3001 accgaatctc gtattcagct tgacgatgct ttcgctgagg tttctcaaat agagaattat
     3061 gttgtagctg agcgttcggc tatgcctgaa tcagaccaag atttaatgac agtatcgctt
     3121 aaaattgata aagaatgtaa gatgggtata gtaactgata ttaagcaagc tttgagaaga
     3181 gcttatgctt tgaaaatcaa ttattctgct atacctaggg ctaatagtca gttttaattc
     3241 tatattaagt ttaaaaaagt ctcttgattt tatctcaaga gacttttttt gtttatataa
     3301 atattttaaa ttgtaacttt gccgaaactt ttataattat gtacttatta ctgtcttttt
     3361 tattcacatt tacttttccc tttgactctt tttccgaaaa gccggatttg atacaaccta
     3421 tgataatacc ggtggagcta agtgcaaact tcgctgaatt acgacctaat cattatcatt
     3481 cgggtgtgga ttttaaaact atgcagataa taggtcaaaa tgtatattca gtagatgatg
     3541 gctatgtagc aagaataaac atatccggaa gcggatatgg gaacgcccta tatattaatc
     3601 atcccaagtc gggtttgaca tctgtatatg ctcatcttga taattttgaa tcatttatcg
     3661 actcaattat taaactaaaa caatatgctg atgaatcttt tgaggtggat ttttacctaa
     3721 gtccggatca attgcctgtt agtaaaggcc agagagttgc gaagagtgg
//
LOCUS       DAOPID010000155         3758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_33468_length_3758_cov_5.554955, whole genome shotgun sequence.
ACCESSION   DAOPID010000155 DAOPID010000000
VERSION     DAOPID010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33468_length_3758_cov_5.554955"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1667)
                     /locus_tag="PK641_05560"
     CDS             complement(<1..1667)
                     /locus_tag="PK641_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HPG55848.1"
                     /translation="MSVKLSKVTKDLNVGLSTAVDFLHQKGFADVEENPNARITDDQF
                     DLLHKEFCKDKDQKIESEIMAMRHKEKSKSESVVLEGFKQTQPEEIKVEIADNERPQL
                     KTVGKIDLESLNYKKSEPQPKAEPVAPVEPVKEEKNVEKVEAPIQKEIIVEPKIEKEQ
                     VKKIEPLEDSKANPVVEPKLESKIREEIQNTTNIEQGSDNTQELDNIENEVSKPAEEI
                     FSMSKPPIQSTINVVGKIDLASLNQQTRPKKKTKEEKRKEREVKYPSKQNSGNNTNSN
                     AANTQNSQNNQNRPQNSPNNAPNANAGAGGDEKRKRNRIQKERIDINKPIDDKSRKPS
                     NNKFSKDKRPFEHAQPNEEDVQKQVKETLALLTGKSKSKTSKYRRDKRDATLQRMQNE
                     AEAELLEGKTIKITEFVTVNELATMMNVAVTQVIATCMNLGLMVSINQRLDAETINIV
                     ADEFGFKTEYISAEVSETINEVQDVPEELVPRAPIVTVMGHVDHGKTSLLDYIRKANV
                     IAGEAGGITQHIGAYNVTLDNGQHITFLDTPGHEAFTAMRARGTKVT"
     gene            complement(1754..3007)
                     /gene="nusA"
                     /locus_tag="PK641_05565"
     CDS             complement(1754..3007)
                     /gene="nusA"
                     /locus_tag="PK641_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPG55849.1"
                     /translation="MAKDSTKSLNMIETFSEFKDLKNIDQSTMISVLEESFRSVIAKM
                     FGTDENFTVVINPTKGDCEIWRDRVVVEDDAVEDPNLQIGITDAKKIDEECEVGEDVT
                     DAVDFLSFGRRAVLNLRQTLYSKILELQKESVYEKYKDMIGEVVYGEVYQIWKKEILL
                     LDAQNNELLLPKTEQIPSDFYRKGDTVKAVVVKVDNQNNTLKIIVSRTAPVFLQRLFE
                     LEVPEVQEGIITIKKIARIPGERAKVAVESFDDRIDPVGSCVGVKGSRIHSIVRELRN
                     ENIDVINYTSNISLFISRALSPAKILSIKVDEANKRAEVSLKPEEVSLAIGKGGLNIK
                     LASMLTEYTIEVFRDIQEDNEEDIYLDEFSDEIEQWVIDVFKELGYDTAKSVLTVSRE
                     QLLKETDLEEETIDQVLSVLKAEFA"
     gene            complement(3015..3467)
                     /gene="rimP"
                     /locus_tag="PK641_05570"
     CDS             complement(3015..3467)
                     /gene="rimP"
                     /locus_tag="PK641_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HPG55850.1"
                     /translation="MINKDVVYQLVAEFLQDKDCFLTDVSVGKDNAIVVEIDSERGVD
                     IDTCCEINKFIELNLNRDIEDCSLEVGSAGITSPFKVKKQYEKNIGNEVEVLSGGKKI
                     FGILKEVGDEHFVVEVEKKEKGSRIVNHEQLRFSYTEVKYTKYNLKFN"
BASE COUNT         1071 a          752 c          517 g         1418 t
ORIGIN      
        1 tccgtaactt ttgtaccacg cgcacgcata gctgtaaacg cttcgtgacc cggggtatcc
       61 aagaaagtaa tatgttgacc gttatctaag gttacattgt aagcaccaat atgttgtgta
      121 attccacctg cctcacctgc aattacatta gctttacgaa tataatcaag caatgaagtt
      181 ttaccatggt caacgtgtcc cataaccgta acaataggag cacgtggaac taattcttca
      241 ggcacatctt gtacctcgtt tatagtttca gatacttctg cactaatata ttctgtctta
      301 aagccgaatt cgtcagcgac tatatttatt gtttctgcat ctagacgttg gttaatagaa
      361 accatcaaac ctaaattcat acatgtagca ataacttgag ttacagctac attcatcata
      421 gtagctaact catttactgt aacaaattcg gtaatcttaa tagttttacc ttccaaaagt
      481 tcagcctctg cctcattttg catacgctga agagttgcat cacgcttgtc acggcgatat
      541 tttgatgttt tagactttga tttacccgtg agcagtgcta aagtctcttt tacttgcttt
      601 tgtacatctt cttcatttgg ttgtgcatgt tcaaaaggac gtttatcttt tgaaaatttg
      661 ttattcgatg gtttgcgaga cttatcgtct ataggtttat taatgtcaat tcgctccttc
      721 tgaattctat tacgttttcg tttttcatct ccacctgctc ctgcatttgc atttggagcg
      781 ttatttggag aattttgagg acgattttga ttgttttgag aattctgagt attagctgca
      841 ttactatttg tgttattacc tgaattctgc tttgaaggat attttacttc tctttcttta
      901 cgtttttctt cttttgtttt cttctttgga cgtgtttgtt gattcaaact agccaaatca
      961 attttaccta caacatttat tgtggattgt ataggaggtt tgctcattga aaatatttct
     1021 tcagcaggtt ttgatacctc attttcaata ttatccaact cttgagtatt atctgaccct
     1081 tgttcaatat ttgttgtatt ttgaatttct tcccttattt tcgattctaa cttaggctct
     1141 actacaggat tcgctttgct atcttctaaa ggctcaattt tcttgacttg ttctttttct
     1201 attttaggtt caacaataat ttctttttga atcggagcct caactttttc tacatttttt
     1261 tcctctttaa ccggttctac aggtgctacc ggttctgcct taggttgagg ttctgatttc
     1321 ttataattaa gactttccaa atctattttg ccaacagtct ttaattgagg tctttcatta
     1381 tctgcaattt cgaccttaat ttcttccggt tgtgtctgtt tgaatccttc aagaacaaca
     1441 gattcacttt ttgatttttc cttatgacgc attgccatta tttcagactc aattttttga
     1501 tccttatcct tgcaaaattc tttgtgcaat aaatcaaact ggtcatcagt aatacgcgca
     1561 ttcggatttt cttccacatc ggcaaagcct ttttgatgta gaaaatcaac agctgtggac
     1621 aaacccacat tcaaatcttt tgttacttta cttagtttta cagacatata ttattttttt
     1681 tctaattctg ttgttattca tttaatccga tacaccctac taaaaacacc gtttttgcaa
     1741 aacaataatg gttttatgca aattcagctt tcagtacaga taatacctga tcaatagttt
     1801 cttcttccaa atctgtttcc ttcaataatt gttcacgaga aactgttaat acactcttag
     1861 ctgtatcgta tcctaattct ttgaacacat caataaccca ctgttcaatt tcatcgctaa
     1921 actcatcaag ataaatatct tcttcattat cttcttgaat atcacggaat acctcaattg
     1981 tgtattcagt aagcatgctt gccaatttta tatttaaacc gcctttaccg attgccaaag
     2041 atacttcttc cggtttcaat gaaacttctg cacgcttgtt tgcttcgtca acttttattg
     2101 acaatatttt tgcagggctc aaagcacgtg atataaacaa tgatatgtta gatgtataat
     2161 taataacatc aatattttcg ttgcgtaatt cacgtacgat actatgtata cgcgaacctt
     2221 taacaccaac acatgaacct acaggatcaa tacgatcatc aaatgattca acggcaactt
     2281 ttgcacgttc accagggatt ctggcaatct tcttaattgt tataattccc tcttgaactt
     2341 caggaacttc caattcgaat aagcgttgta aaaataccgg agctgttctt gatactataa
     2401 ttttcagagt attgttttga ttatcaactt ttaccactac agccttaact gtatcgcctt
     2461 ttctgtaaaa atcagaagga atttgttctg ttttaggtaa aagcaattcg ttattttgtg
     2521 catctaacaa caatatctct tttttccaga tttgataaac ttcgccataa acgacttcac
     2581 ctatcatatc tttatatttt tcgtaaacag attctttttg tagttctaat attttagaat
     2641 acaatgtttg acgcaaattt aaaaccgcac gacgaccaaa tgacaaaaaa tccactgcat
     2701 ctgtaacatc ttcaccaacc tcgcactcct catcaatttt cttggcatca gttataccta
     2761 tttgcaaatt aggatcttct acagcatcat cttcaacaac tactctatca cgccatattt
     2821 cgcaatcgcc tttagtcggg ttaataacca ctgtaaaatt ttcgtctgta ccgaacattt
     2881 ttgcaataac gctgcgaaac gattcttcca aaacactaat cattgttgac tgatcgatgt
     2941 tctttaaatc cttaaactct gagaaagtct caatcatatt tagagacttc gttgaatctt
     3001 ttgccatttt agtgtcaatt gaattttaag ttatattttg tgtattttac ctctgtataa
     3061 gaaaatcgca attgttcatg atttactatg cgactacctt tttctttttt ctctacttct
     3121 acaacgaaat gttcatctcc aacttctttt aaaattccga agattttttt accaccggac
     3181 aagacttcta cttcgtttcc gatatttttt tcgtattgtt tttttacttt aaacggagat
     3241 gtaatgcctg cagaaccaac ttcaagacta caatcttcaa tatcacgatt aagatttagt
     3301 tctataaatt tattaatttc acaacatgta tcaatgtcaa cacctcgttc tgaatcaatc
     3361 tccacaacta tggcattgtc tttacctact gacacatcag tcagaaagca atctttatct
     3421 tgcaagaact ctgcaacaag ttgatataca acatctttat ttatcattcg gtatattaca
     3481 ataaagaagg ggaacttacg tcccccctca aatcggtgca aaggtatgaa taattattta
     3541 aaacacaaaa tattacaatt atttttataa cccagtaaat tgaaaattca aactctttaa
     3601 aacgatatcg aaaaatacca ctttgtacat ttaataaatt atgtaccttt gttgctgtgt
     3661 tataagaatc agagtacaat tctgcttgtt gtttcaaata tggagcatta caattacaat
     3721 cataataaat taggagtctt agatgtttca atacgtcc
//
LOCUS       DAOPID010000156         3743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_33662_length_3743_cov_5.095445, whole genome shotgun sequence.
ACCESSION   DAOPID010000156 DAOPID010000000
VERSION     DAOPID010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33662_length_3743_cov_5.095445"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="PK641_05575"
     CDS             complement(<1..432)
                     /locus_tag="PK641_05575"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPG55851.1"
                     /translation="MKTKSIFSALCGAAVIAMAFSSCGPEPTQFTLSLDKATASIGVG
                     SNTTITATITPEVVGATVTFTSSNEAVATVVGTMQSCTVTGVSEGTAVITATYEGVSK
                     TCAISVSDVQVDPSLLGTDYYVIQLDAQSREFLGNKIVADYA"
     gene            complement(472..2103)
                     /locus_tag="PK641_05580"
     CDS             complement(472..2103)
                     /locus_tag="PK641_05580"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPG55852.1"
                     /translation="MKKLNHILILSLMLSVVSCSDWVHEPSPNVTTLEDFYQSQESAI
                     QNVNAAYVPLMWEYNSTYFSEFFIGDVVSDDALKGGQYVMTDMMDAYEMENWKTNTNN
                     SLLRDYYRAKFQGIGRCNLVLKYVPEMDNSLFTDGVKERVIGEAKFLRAYYYFQLVRV
                     FGGVPLSLEVISSSEKWQMPRSTREAVVEQILSDLTDAEAVLWEKSDSRFTPEDLGRA
                     TKGAAQAMLLKVNLYKAGLIDNSAYEEAYNWGHKLTRESTEYDLFPLYEDNFTLAGEN
                     GIESVFEVQYGQEPTSDYGEGYGFTRGTFQVILTRTRSSLAFGETGWGFNRPTQNLYD
                     EYEESDPRRDITIFNPTNEQIENPAEEIYLGDRYCSRKYAMYYNDGTCYHLDHPTRGP
                     INRKDIRFSDVLLMYAEACEATTRTSEAEWALERVRSRARSNASEDAINPLPEYPNYT
                     IKINGVEKTPTLKEAIRHERRMELAMEGHRWYDLCRWKGFDGSGVKQHMDAYKLTETP
                     EAQEHMGDFVAGKHELFPIPSQEIDLNPMDQNPLY"
     gene            complement(2122..>3743)
                     /locus_tag="PK641_05585"
     CDS             complement(2122..>3743)
                     /locus_tag="PK641_05585"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPG55853.1"
                     /translation="NKYEYSSFDKSNKNFLESSMANSMTFQNDNIIQYSRDIQKNRFS
                     IMLGQSLEEKSSYSIGGSGASIPVPDKYHWYLSQTTEDRTYASDGASRTRMFSLFSRL
                     FYSYDGKYMVTVNFRADNTSRFTENPWGYFPSAALSWNLSEEKFMKRFSKMDYLKIRG
                     GWGMLGNQEIGQDDFTQLMFTSGPTFVDYILGADQLYENALGATVLTYVNTGGKWETT
                     SQWNVGIDFGFWNGMLTGTIDGYIRNTSDMLMSVNAPAQVGNRYSAIKNVGSVLNEGI
                     ELSLGHRNSVGDFSYSIDGNISFVKNELTALNGGSTVYLDGIRVCDEGLPLYTIWGYK
                     YLGVYKSNEEANTYLPNSSIPVSAGDSKYEDLNGDGNIDDADKANLGNPFPWLTYGLN
                     FSADYKGFDLQLFFQGVVGNELYNALRVRTEGSGTECTLSTTMRDVWSTSNPDGSIPN
                     PKNSYNSQVSSRFVESGDYFRLKNLQLGYTVPKRIMSKAHINRLRFYVSISNLFTITD
                     YSGYDPEVAGGVDYGNYPQARTFLFGLNLDF"
BASE COUNT         1185 a          785 c          589 g         1184 t
ORIGIN      
        1 agcataatca gctacaattt tattacctaa aaattctctg ctttgagcat caagttgaat
       61 tacgtaataa tcagtaccta ataaagaagg atctacctgt acatcactaa cactgatagc
      121 acaagtttta gaaacacctt cgtaagttgc agtaataact gcagtacctt ctgatacacc
      181 tgttactgta caagattgca ttgttcctac aactgttgca actgcctcat ttgaagaagt
      241 aaatgtaaca gtagcaccta ctacttcggg tgtaattgta gcagtgatag ttgtgtttga
      301 accaacacca attgatgctg tagctttgtc taatgacaat gtaaactgcg tgggctccgg
      361 accacaacta gagaaagcca ttgcgataac ggctgcaccg cataacgcac taaaaatact
      421 ttttgttttc atgataataa attaaagtta aaaaataaat aaactattat tttaatatag
      481 aggattctga tccatagggt tcaaatcaat ctcttggctt ggtattggaa ataattcgtg
      541 ttttcctgct acaaaatctc ccatatgttc ttgcgcttca ggagtttctg taagtttgta
      601 agcatccatg tgttgtttaa cgccactgcc gtcaaaacct ttccaacgac acaaatcata
      661 ccagcgatga ccttccatcg ccaactccat acgacgttcg tgacggatag cttcttttaa
      721 agtaggagtc ttttcaacac cattgatctt gatagtataa ttaggatact caggtagagg
      781 atttattgcg tcttcagatg cgtttgaacg agcacgcgaa cgaactcttt ccaaagccca
      841 ttcagcttca gatgtacgag ttgttgcctc gcatgcttct gcgtacatta ataatacatc
      901 ggaaaacctt atatcttttc tgttaatagg accacgagta gggtgatcta aatgataaca
      961 agttccgtca ttataataca tagcatattt tctgctgcaa tatctatcgc caagatagat
     1021 ttcctcagcc ggattttcta tctgctcatt tgttggatta aagattgtaa tatcacgacg
     1081 tggatcgctt tcttcatact catcatataa gttttgtgtt ggtctattaa atccccagcc
     1141 ggtttcacca aacgccaaac tagaacgagt acgggttaaa attacttgga atgttccgcg
     1201 tgtaaatcca tatccttcac cataatcact agtaggttct tgaccatatt gtacttcaaa
     1261 tactgactcg ataccatttt cgcctgctaa agtgaagtta tcttcatata atgggaaaag
     1321 atcatattcc gttgactccc tagttaattt atgcccccaa ttgtaagctt cttcgtatgc
     1381 agaattatca attaaaccag ctttatacag attaactttt aacaacatag cctgcgctgc
     1441 tccttttgtt gcacgaccaa gatcttcagg tgtaaatcta gaatctgatt tttcccataa
     1501 aactgcttct gcatctgtaa gatccgataa tatttgctca acaacagctt ctcttgttga
     1561 tcttggcatt tgccattttt ccgaactcga aataacctct aaggaaagtg gaacaccacc
     1621 aaaaactctt actaactgga aataataata agcacgcaag aattttgctt cgccaattac
     1681 acgttcctta actccgtccg taaataaaga gttgtccatc tccggaacat atttcaacac
     1741 taaattacaa cgaccaattc cttgaaattt agcacgataa taatccctca ataaggaatt
     1801 attcgtattt gtcttccaat tttccatttc gtacgcatcc atcatatcgg tcataacgta
     1861 ctgaccacct tttaaagcat cgtcagacac tacatcacca ataaagaatt ctgaaaaata
     1921 ggtcgaatta tactcccaca ttaatggtac gtatgctgcg tttacatttt gtattgcaga
     1981 ctcttgcgac tgatagaagt cttcaagagt tgttacattg ggacttggtt catgaaccca
     2041 atccgaacac gacaccacac taagcatcaa tgacaatatc aatatatgat ttaacttttt
     2101 cattttcgtg tgtttttaat attagaaatc aagatttaag ccaaataaaa atgtgcgagc
     2161 ctgcggataa ttaccataat caacaccgcc tgcaacttca ggatcataac cagaatagtc
     2221 ggttattgta aacaaattgc ttatcgacac ataaaaacgc aagcgattaa tatgagcttt
     2281 cgacattatg cgtttaggaa ctgtatatcc caattgaaga tttttcaaac ggaaatagtc
     2341 accactttca acaaaacgac ttgatacctg cgaattgtaa gaatttttag gattaggtat
     2401 agatccatca ggattgcttg tagaccatac atcgcgcatg gttgtactca aagtacattc
     2461 agtaccggaa ccttcggttc taactcttaa agcgttgtac aattcgttgc caactacacc
     2521 ttggaagaat aattgtaagt caaagccttt ataatctgca gaaaaattta aaccatatgt
     2581 taaccacggg aatggatttc ccaaatttgc tttatctgca tcatctatat tgccatcacc
     2641 atttaaatct tcatattttg aatcacctgc acttacagga attgaactgt tcggcaaata
     2701 tgtattagcc tcttcatttg atttgtaaac tcctaaatat ttataacccc aaattgtata
     2761 taaaggtagt ccctcgtcgc aaacacgtat tccgtctaag taaacagttg atccaccatt
     2821 taaggctgtt agttcatttt ttacgaatga tatgttacca tctattgaat atgagaaatc
     2881 accaaccgaa ttacgatgac caagagacaa ctcaatacct tcatttaata cagaacctac
     2941 atttttaatt gctgaatatc tatttccaac ttgtgcgggt gcatttactg acattaacat
     3001 atctgatgta tttcttatat aaccatcaat tgtacctgtc aacattccat tccaaaatcc
     3061 gaaatctata ccaacattcc attgagaagt tgtttcccat ttaccaccgg tattcacata
     3121 agtaagtact gtagctccaa gtgcattttc atataattga tctgctccaa gtatataatc
     3181 aacaaaagta ggtccggagg tgaacattaa ttgcgtaaag tcgtcttgac caatttcctg
     3241 atttcccaac ataccccaac ctccacgaat tttcaaataa tccatcttag aaaatctttt
     3301 catgaatttt tcttcactaa gattccacga caaagcagct gaggggaaat atccccaagg
     3361 attttccgta aacctactgg tattatctgc gcggaaattt acagttacca tgtatttgcc
     3421 atcatatgaa taaaacaatc tgctaaacaa tgagaacata cgagtacgac ttgcaccatc
     3481 agaggcataa gtacgatctt cggtggtttg agataaatac caatgatatt tatcaggaac
     3541 cgggattgac gcaccggaac cgcctattga gtacgacgat ttttcttcaa gagattgacc
     3601 caacataatt gagaatctgt ttttctgaat atcgcgagaa tactgaataa tattgtcgtt
     3661 ttggaatgtc attgaattag ccatactgct ttccaaaaag ttcttattgc tcttatcaaa
     3721 agaagaatat tcatatttat ttt
//
LOCUS       DAOPID010000157         3706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_34126_length_3706_cov_3.770748, whole genome shotgun sequence.
ACCESSION   DAOPID010000157 DAOPID010000000
VERSION     DAOPID010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34126_length_3706_cov_3.770748"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1387
                     /locus_tag="PK641_05590"
     CDS             <1..1387
                     /locus_tag="PK641_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HPG55854.1"
                     /translation="FLILLLIAFIGRKDNDNYAFFLGKRQSPWYIVAFGMLGASISGI
                     SFISVPGMAAYSGFTYMQMVVGFSIGYVVIAYLLLPLYYKIGTTSIYEYLGMRFGIHS
                     HRTGTCFFILSRALGGAARIFVAAVIIQTFVFGHWNIPFWLTSVILISLIYLYTFTNG
                     IKTIVWTDALQTFFLLSALIVLFIQAFPLVSFKNVEIPQFVVFDINSKADFFKMLLSG
                     IFITITMTGLDQDMMQKNLSCKSLRDAKKNMLSYGAAFIPINLLFLFLGWMLCIIYAN
                     NGQALPANTDNILPDAISLSYLGQGATIFFILGIVSATFSSADSTLISLTTSVSVDLL
                     HSEQKQEKTAKRIRQYTHIFMCLLFVFLMIIFNSIDNRNIIDVLFKIASYTYGPLLGL
                     FTFGMFTKRKVHDKFVPIIACLSPIICLIIQSVTNNFFGYELLIINGLITFLGLIISS
                     IIYENKNRHLC"
     gene            1359..1955
                     /gene="yihA"
                     /locus_tag="PK641_05595"
     CDS             1359..1955
                     /gene="yihA"
                     /locus_tag="PK641_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HPG55855.1"
                     /translation="MKIKTATFVKSCADITQCPKEELLEYAFIGRSNVGKSSLINNLT
                     NIKGLAMTSSKPGKTLLINYFLINEEWNLVDLPGYGYAKVSKDIRKNIEIMIKNYIMQ
                     REQLTNLFVLIDSRLEPQKIDLEFISWLGEMGIPFCIVFTKADKLTNNGLKVNIENYK
                     TKLLENWETLPPLFISSAETGLGKDEILDYIDHINASV"
     gene            2025..3455
                     /locus_tag="PK641_05600"
     CDS             2025..3455
                     /locus_tag="PK641_05600"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPG55856.1"
                     /translation="MSNWFYAILSLPATAMGFALSVQISALSWILSTKYGLNIEDIGL
                     VWAAGPIAGIIAQPIVGALSDNMWFMGGRRRPWIIIGGILSGLMMLALPNLEVVQKIF
                     VGGEDPQAGLLSIAVIVALTFDLSINVSFNPTRSLIADCTSMENRAKGYTWMQTISGS
                     FGVLAYFIGGVLGNIALIYIGIGIALLATIIPAFLIEEPRELQQVVTDDAKKSKASAR
                     DFIESLLPLIGLLVYGIYIICEKLIGFNITYGIEIFGFFINIVECICLALIVLSAFIV
                     LPNLLGSNKNSNEFQKVLFAHSFTWIGVQTMFVYAFFYLRESMGLDEKMAGSVNSIAF
                     FILSLVSAILPILVLNGSAKRFGMVRTHTICILIMALGYFGIYLFGTSSVISYYALMA
                     IAGIGWASVVSLPFAIMSEKVDQTKMGLYMGIFNLSVVLPQLVASFKMGQIINATADK
                     TIIFAICAACLFISGIFWFTVKESKK"
BASE COUNT         1126 a          602 c          661 g         1317 t
ORIGIN      
        1 ctttctaata cttttgctta ttgcgtttat tggcagaaaa gacaatgaca actatgcgtt
       61 ctttttaggg aagaggcaat caccatggta tattgtagct ttcggcatgt tgggagcttc
      121 aatatcaggc atctctttta tatcagtgcc ggggatggct gcatattcag gttttacata
      181 tatgcaaatg gttgtaggtt tttcgatagg atacgtagtt attgcatact tattgctccc
      241 actctactac aaaataggca ctacaagcat ttatgaatac cttgggatga gatttggcat
      301 acatagccac cgtacaggaa cctgtttttt tatactctca agagcattag gaggagccgc
      361 acgtattttt gtggcagctg tgattataca aacttttgta tttggtcatt ggaatattcc
      421 attttggctg acttctgtaa ttttaataag tttgatatac ttatacacct tcactaatgg
      481 cattaaaacc attgtttgga ctgatgcgct acaaacattt tttcttttat ctgcattaat
      541 agtcttattt attcaagctt tccctttagt aagtttcaaa aatgttgaga ttcctcaatt
      601 tgttgtattt gatataaatt cgaaagccga tttctttaaa atgctactta gtggtatttt
      661 tattacaatc actatgacag gcttagatca agatatgatg caaaaaaatt tgagttgcaa
      721 gagcttacgc gatgccaaaa aaaacatgct tagttatggt gccgcattta tacctattaa
      781 cctattattt cttttcttag gatggatgct ctgcattatt tatgcaaaca acggccaagc
      841 tttaccggcc aataccgaca atattttacc cgatgcaatt tcactttcgt atcttgggca
      901 gggtgctaca atatttttta tactaggaat tgtgtctgct acattttcga gtgcagactc
      961 tactttaata tcattaacga cttctgtttc ggtagatttg ctacactctg aacagaaaca
     1021 ggaaaagaca gctaaacgca tcagacaata tactcatatc tttatgtgtt tattgtttgt
     1081 ttttttaatg attatattta actctattga taaccgaaat ataattgacg tgttatttaa
     1141 aattgcatca tacacttatg gtccattact tggattattc acttttggta tgtttacaaa
     1201 aagaaaagta cacgacaaat ttgttccaat aattgcctgt ttatctccta ttatttgttt
     1261 gattatacaa tctgtcacaa ataatttctt tggatacgaa ttattaatca taaacggctt
     1321 aattactttt ttaggtctaa tcatttcatc tattatatat gaaaataaaa accgccacct
     1381 ttgttaaaag ttgtgctgac ataacacaat gtccaaaaga agagctactt gaatatgcct
     1441 tcattggtcg ttcaaatgtt gggaaatcat cattaattaa taatttgacc aatatcaaag
     1501 ggcttgccat gacaagttct aaacccggaa aaacattatt aataaattat tttctgataa
     1561 acgaagaatg gaatttagtt gatcttcccg gatatggata tgcaaaagta agtaaggata
     1621 ttcgcaaaaa tattgaaatc atgattaaga attacatcat gcaacgcgag caacttacta
     1681 atctatttgt gcttatagac agccgtttag agcctcaaaa aatagacttg gagtttattt
     1741 catggcttgg agaaatgggc attccattct gcattgtttt tacaaaagca gacaaactaa
     1801 ctaataatgg attaaaggta aatattgaga attacaagac aaaactttta gaaaattggg
     1861 aaacacttcc acctcttttt atatcttcag ccgaaacagg acttgggaaa gatgagattt
     1921 tggattatat cgaccatatt aacgcatcag tgtaaaaaaa tatatttacc tttgccaaac
     1981 ttataactct taaaaacaga actatgctaa acatgcagaa aaaaatgagt aattggtttt
     2041 atgccatact cagtcttccg gctaccgcaa tgggattcgc cttatcggtg caaatttcag
     2101 ctttaagttg gattctatca acaaaatacg gtttaaatat tgaggatatc ggcttggttt
     2161 gggccgctgg tcctatagcc ggtataattg cgcaacccat agtcggtgct cttagtgata
     2221 atatgtggtt tatgggaggc agacgcaggc cttggattat tattggtgga attttgtcag
     2281 gtttaatgat gcttgcactt cctaacttag aagttgtaca aaaaatattt gtcggaggtg
     2341 aggatccaca agcaggtttg ctttctatag ctgttattgt agcacttact tttgacttga
     2401 gtataaatgt aagtttcaat ccaactcgtt ctttaatagc cgattgtacc agcatggaaa
     2461 accgtgccaa aggatatact tggatgcaaa caatttccgg ttcgtttggt gtactcgcgt
     2521 attttattgg cggtgtgctt ggtaacatag cattaattta cattggtatt ggcattgcgc
     2581 ttcttgcaac gataatacct gcattcttaa tagaagaacc tcgcgaatta caacaagtag
     2641 taactgatga cgcaaaaaaa agtaaagcgt ctgcacgcga ttttattgaa tctttattgc
     2701 ctcttatagg tttgcttgta tatggaattt atattatctg cgaaaaactt attggtttca
     2761 acataactta tggtattgaa attttcggat tttttataaa tattgttgaa tgtatttgcc
     2821 ttgcattaat tgttttaagt gctttcatag tacttccaaa tttattaggc agcaacaaga
     2881 attcaaacga gtttcagaaa gtactgtttg ctcattcatt tacttggata ggcgttcaaa
     2941 ctatgtttgt atacgcattt ttctatttaa gagaaagtat gggacttgac gaaaaaatgg
     3001 ccggcagcgt aaactctata gcatttttta ttttgagttt ggtaagtgct attttgccta
     3061 tccttgttct taacgggtct gctaaacgct ttggtatggt tcgtacgcat acaatatgta
     3121 ttcttattat ggcattggga tattttggaa tttacttatt tggaacatct tctgtaatat
     3181 cttattatgc gcttatggca attgccggta ttggttgggc ttcggtagta tctttgccat
     3241 ttgctattat gagcgaaaag gttgatcaaa ctaaaatggg tttatatatg ggaattttca
     3301 atttatcggt tgtgttacca caattggtgg catcatttaa aatgggacaa attattaatg
     3361 ccacagccga caaaactatt attttcgcta tatgtgctgc atgcttattt atctcaggaa
     3421 tattttggtt taccgtaaag gaatcgaaaa aataatcaca aaacataaat tataaaaaac
     3481 ggggttcaca tcaaaatgtg agccccgttt ttgtatctcc aatatattta aaattatttt
     3541 gttagctgta tgcctaccgg taatgaatat atcttagtat tgaagtttaa ttcggtttcg
     3601 cttagggatg tgatataaca atttttatcc attgattcaa ttagctgaca atctaatatc
     3661 aattttgtac cctcccgact ccatgtacct tgtatcgtat tgttag
//
LOCUS       DAOPID010000158         3611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_35441_length_3611_cov_4.070022, whole genome shotgun sequence.
ACCESSION   DAOPID010000158 DAOPID010000000
VERSION     DAOPID010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35441_length_3611_cov_4.070022"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /gene="dusB"
                     /locus_tag="PK641_05605"
     CDS             complement(<1..905)
                     /gene="dusB"
                     /locus_tag="PK641_05605"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HPG55857.1"
                     /translation="MRIAQIEFPSYPLFLAPMEDVTNPNFRLLCKRYGADMVYTEFIS
                     ADALIRNVPRTEQKLTVADAERPVVIQLYGKDPEAMADAAKRAEEANPDIIDINFGCP
                     VKKVAGKGAGAGLLKDIPRMLEITRAVVASVKTPVTVKTRLGWDDNNKIIVDLAESLQ
                     DCGIAALAIHGRTRAQMYRGDADWTLIGVVKNNQRMHIPIIGNGDVTTPERAKECFDR
                     YGVDAVMVGRAAIGKPWVFKEMKYFLETGEHFEGFSPSEQMDILKEQVLKSVEWLDER
                     RGILHIRRHIAVTPLFKGIPNFKDTR"
     gene            976..1629
                     /locus_tag="PK641_05610"
     CDS             976..1629
                     /locus_tag="PK641_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPG55858.1"
                     /translation="MNKKHISTKENILQTTIDLLNKKGLQNVTFRDVADALHISLGNI
                     TYYFHKWDNLMDVIFTDYCVKDVKELYLYFPNNIHEIGQYLSRIYDVQIKYTFVFSNF
                     YLFFNVFPRYNHFKEEFFIDRMDKMRLGFVHLAEQNYMYPPSKDHDYDLLTKDMWVFV
                     ANWYGFSRMFNDSPNRITKEQFFMSIYNISVFHLTEEGKIAILESFDKLRKEGVFDK"
     gene            1629..2867
                     /locus_tag="PK641_05615"
     CDS             1629..2867
                     /locus_tag="PK641_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HPG55859.1"
                     /translation="MQISDYEIMAPVGSWESLAAALNAGSDSVYFGIEHLNMRSHSSN
                     NFTTEDLKEIVRRCKEKGVKTYLTVNTILYDADLDLMHEIIDIAKASGISAIIASDIA
                     AMQYACQIDVEVHLSTQLNISNIEALRFYAQFADVVVLARELNLDQVKAIHEAIVREN
                     IVGRHGKLIKIEMFCHGALCMAVSGKCYLSLHEYDRSANRGECMQICRRGYTVTDKET
                     GSELDINNKYIMSPKDLKTIHFINKMMDAGVQVFKIEGRARGAEYVKTVVSCYKDAIS
                     SYVNGTFTEDKIVEWDKQLATVFNRGFWNGYYLGQRLGEWSSNYGSQATKRKVYIGKI
                     TNYFKKINVAECLMETQDLKLGDEILITGETTGAYIATIDELRMDDSPVQAVVKADVF
                     SIKTSLPVHRNDKLFKLVEA"
     gene            2867..>3611
                     /locus_tag="PK641_05620"
     CDS             2867..>3611
                     /locus_tag="PK641_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55860.1"
                     /translation="MASKRKKIILSICIPVLVIAFLLALPIVALRIALTESVSNKLLG
                     MLPDYMQAEASIKKIDYSIFATWPNIEIQLSDVILISDVFEPKDTLLQIKHLDLSASA
                     GAFLSEGKIQVNRCILDEPCVYGHSIEDKNNWDVLPPSEPDTSSSTLPDIYFTKIEIN
                     KANLKYKDETQKLDAQVSGLSLQVPKGVYTPDSLAAQLAMSINSIIYKDGTQKLDAQV
                     LGLSLQVPEGTYTPDSLAAQLAMSINSIIY"
BASE COUNT         1214 a          648 c          671 g         1078 t
ORIGIN      
        1 cgcgtatcct taaaattagg aatcccctta aaaagaggcg ttactgctat atgtcttcgt
       61 atatgcaaaa tgcctcttct ttcatctaac cactcaacac ttttgagaac ttgctctttt
      121 aaaatatcca tttgttcgga aggtgaaaag ccttcaaaat gctcgccggt ttcaagaaaa
      181 tatttcattt ctttaaacac ccaaggtttt ccaatagcag cacgccctac cattaccgca
      241 tctacaccat aacgatcaaa gcattctttt gcacgctcag gagtagttac atcaccattc
      301 ccaataatag gaatatgcat acgctgatta ttcttaacaa cgcctataag agtccaatca
      361 gcatcaccac gatacatttg agctctcgta cgaccatgaa tagctaatgc agcgattccg
      421 caatcttgca aactctctgc taaatctaca atgattttat tattatcgtc ccaacctaaa
      481 cgagtcttaa ctgtaacagg agtctttacg gaagcaacta cagcacgagt tatttcgagc
      541 atacgcggta tgtctttgag caaacctgca cctgcacctt tgccggcaac cttttttacc
      601 ggacatccga aattaatatc tataatatca ggatttgctt cttcggcacg ttttgcagca
      661 tcagccattg cctcagggtc tttcccgtat agttgaatta caacaggacg ttcggcatca
      721 gctacagtta atttttgctc agtgcgaggc acatttctaa taagtgcatc agccgaaata
      781 aattccgtat aaaccatatc tgcaccatat cgcttacaca ataagcgaaa attaggattg
      841 gtaacatctt ccattggggc taagaataat ggataagaag ggaactcgat ttgtgctatc
      901 cgcattgact taatattttg ttgcaaagat aaaaaaagaa ctactaaatt cacatttttt
      961 gtgtatgttt gtggcatgaa taaaaagcac atttcaacaa aagaaaacat attgcaaacc
     1021 acaattgatt tactcaataa aaaaggtttg caaaatgtaa catttaggga tgttgcagat
     1081 gcgcttcata taagtttggg taacatcact tattactttc ataagtggga caatttaatg
     1141 gatgtcatat ttacagacta ctgcgtaaaa gatgtaaagg aactgtattt atattttccg
     1201 aataacattc atgaaattgg acagtacctt agcagaatat atgatgtaca aattaaatac
     1261 acatttgtat tttctaattt ctatttattc ttcaatgtat ttccacgcta taaccacttt
     1321 aaagaggagt tttttataga ccgaatggat aagatgcgtt taggatttgt acatcttgcc
     1381 gaacaaaatt acatgtatcc accaagtaaa gatcatgatt atgacttact gaccaaagat
     1441 atgtgggttt ttgttgctaa ttggtatgga ttttcgcgta tgtttaacga tagtccaaat
     1501 agaatcacca aagaacaatt ttttatgtct atatacaata tttcggtatt tcacctaact
     1561 gaagaaggca aaattgctat acttgaatcg tttgacaaat tgcgtaaaga aggagtattt
     1621 gataaataat gcagatttcg gattatgaaa taatggcacc ggtaggttct tgggaaagcc
     1681 ttgcagcagc attaaatgca ggatcggact cggtgtattt tggtattgag catctaaata
     1741 tgcgcagtca ctcatcaaac aattttacta cagaagattt aaaagaaatt gttagaaggt
     1801 gcaaggaaaa aggagtaaaa acatacctga ctgtaaatac cattttatat gatgccgact
     1861 tagatttgat gcatgaaata attgacattg caaaggcatc aggcatttct gccataattg
     1921 catctgatat tgccgctatg caatatgcat gtcaaataga tgttgaagta cacctatcta
     1981 cccaactgaa tatttcgaat atagaagccc ttcgttttta tgcgcaattt gctgacgtag
     2041 tggtacttgc acgcgaactt aatctagatc aggtaaaagc catacacgag gctattgtgc
     2101 gtgaaaatat agtgggcaga catggaaaac taataaaaat agaaatgttt tgccatggtg
     2161 cattatgcat ggctgtatca ggaaaatgct acttgagttt gcacgaatat gatagatctg
     2221 caaacagagg tgaatgtatg caaatttgta gacgcggata tacggttaca gataaagaaa
     2281 caggatcgga acttgatatt aacaacaaat atataatgtc gcccaaagac ttgaaaacga
     2341 tacattttat aaataaaatg atggatgcag gcgtacaagt atttaaaatt gaggggcgcg
     2401 cacgtggagc agaatatgta aagacagtag tatcttgcta taaggatgcc ataagcagct
     2461 atgttaatgg gacctttact gaagataaga ttgttgagtg ggataagcaa cttgctactg
     2521 tttttaatcg tggattttgg aatggatatt atttgggaca acgtttgggc gaatggagtt
     2581 caaattacgg ctcacaagct acaaaacgta aggtttacat aggtaaaatt actaactatt
     2641 ttaaaaagat aaatgttgca gaatgcttaa tggaaactca agatttgaag cttggtgatg
     2701 agattcttat tacgggagaa acaaccggtg catatattgc aactatagat gagttacgta
     2761 tggatgacag tccggtacaa gcagtggtaa aagccgatgt gttttcaatc aaaacatcat
     2821 tacctgtaca tagaaatgat aaattattta aattagttga ggcataatgg catctaaaag
     2881 aaaaaaaata atcttaagta tttgcatacc tgttcttgtt attgcatttt tgctggcgtt
     2941 gccgatagtt gctcttcgca tcgctctaac tgaatctgtt agcaacaaat tgcttggcat
     3001 gctacctgat tatatgcaag cagaagcaag tatcaaaaaa attgactata gcatttttgc
     3061 cacatggcct aacatcgaaa tacaattatc tgatgtaatc cttatatctg atgtatttga
     3121 gcctaaggat actttactac agatcaaaca cttagactta tctgccagtg ccggtgcctt
     3181 tttgagcgag ggcaaaatac aagtaaatcg ttgcatactt gacgagccat gcgtttacgg
     3241 gcatagtatt gaggataaaa acaattggga tgttttaccc ccatctgaac ccgatacaag
     3301 ttcatcaaca cttcctgaca tttatttcac aaaaattgag ataaacaaag ctaatcttaa
     3361 atacaaggat gaaacacaaa agcttgatgc tcaggtatca ggattatcat tgcaagtgcc
     3421 taaaggagta tatactcctg actctttagc agcacaactt gcaatgagca tcaactcaat
     3481 aatatacaag gacggaacac aaaagcttga tgctcaggta ttaggattat cattgcaagt
     3541 gcctgaaggg acatacactc ctgactcttt agcagcacaa cttgcaatga gcatcaactc
     3601 aataatatac a
//
LOCUS       DAOPID010000159         3577 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_35883_length_3577_cov_4.603066, whole genome shotgun sequence.
ACCESSION   DAOPID010000159 DAOPID010000000
VERSION     DAOPID010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35883_length_3577_cov_4.603066"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="PK641_05625"
     CDS             <1..1108
                     /locus_tag="PK641_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55861.1"
                     /translation="VEKGGKIEAIGTPASPIVFTSNQAAGERNYGDWGGIIICGNGRT
                     NSATGVGKIEGGPRSEYGGNDDYDNSGTLQYVRIEFPGVEYATDNEINGLTFGAVGSG
                     TTIDHIQVSYCGDDSYEWFGGMVNASYLVAYRGWDDEFDTDNGYRGKVQFAVGLRDPN
                     VADKSKSNGFESDNDASGSSNEPFTQAVFSNVSLFGPYTTTSDIVDPQKGNGQFQAAM
                     HLQRNTQLRCYNSIFAGWPIGLFIENGGKGNAQENAINGSLILNNNIMAGMKVNFKED
                     EKGTADFNYTYWNESSRNNTLLTNNIDLGLVDPFNITSPKFYASNSNGVIFGKASFNS
                     DDLSGMTNVNFVGAFDTEDWTDEWCNFDPQNTIY"
     gene            1187..1666
                     /locus_tag="PK641_05630"
     CDS             1187..1666
                     /locus_tag="PK641_05630"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HPG55862.1"
                     /translation="MKKHYLIQLFIACLISISVFAESDIITLSDGIYFKDGSQKELYT
                     GDYKELYPDGSLKLEMRIKNGQIEGTYVTYFPNGNVKEVRSYLDGQLHGTWRTYNSDA
                     ILTAEAIYSHGKKNGTWRIWDDSGKLRYQMNYINNKKIGIWQSWNENGIIVQTRTYK"
     gene            1747..2079
                     /locus_tag="PK641_05635"
     CDS             1747..2079
                     /locus_tag="PK641_05635"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPG55863.1"
                     /translation="MKKKFGVTLMALLYVSCIAVAKIATNSVEKKTSGVLYGYVVDAE
                     AGEPIAGAVIEICNQKVFSDLDGKFEINGLTSNKLQMRVSMISYNTQTVEIDLSKQSN
                     VSISILQQ"
     gene            2076..2780
                     /locus_tag="PK641_05640"
     CDS             2076..2780
                     /locus_tag="PK641_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPG55864.1"
                     /translation="MSPQKRILVVDDESDICEILKFNLEKNNFIVDVAYSAEEALEND
                     LSTYNLFILDVMMGKISGFNFAKITQQKFETAKIPIIFITAKDTIEDKFVGFKIGADD
                     YISKPFSISETLARIENILKRYDENNKHSDINFKRNITTYKGIYIDENSKTVTIDNKP
                     VQLTKKEYELLVLFLHKPNKIFSRNELLQLIWDNDVYVLERTIDVNIARLRNKITPYE
                     DILITKQGYGYKSNLE"
     gene            2855..>3577
                     /gene="tgt"
                     /locus_tag="PK641_05645"
     CDS             2855..>3577
                     /gene="tgt"
                     /locus_tag="PK641_05645"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPG55865.1"
                     /translation="MQFTLQHTDLKSQARAGLLQTDHGTIETPIFMPVGTVGSVKAVH
                     QRELNDDIKAQIILGNTYHLYLRPGINILEKAGGLHKFIGWDKPILTDSGGFQVFSLS
                     DTRKLKEEGVTFRSHIDGSKHLFTPENVVDIERSIGADIIMALDECTPGDADYNYAKK
                     SLELTLRWLDRGVKHFRETEPKYGYHQSYFPIVQGCVYPDLRKKSAEIIAELDMDGNA
                     IGGLAVGEPAEKMYEMIEVVNEI"
BASE COUNT         1295 a          579 c          687 g         1016 t
ORIGIN      
        1 tgttgaaaaa ggaggtaaaa tcgaagctat aggtactccc gcatcaccta ttgtatttac
       61 atcaaatcaa gctgccggag aacgaaacta cggcgactgg ggtggcatca ttatttgcgg
      121 taatggacga accaatagtg ccaccggtgt aggtaaaata gaaggtggcc cacgcagtga
      181 atacggtggt aatgatgatt atgataattc aggcaccttg caatatgtac gcatcgaatt
      241 tccgggcgta gagtatgcta cagataacga aatcaacgga ttaacatttg gtgcagtcgg
      301 atcaggcact acaatagacc acattcaagt atcatactgt ggcgatgatt catacgaatg
      361 gtttggaggt atggtaaatg caagctactt agtcgcatat agaggatggg atgatgaatt
      421 cgacacagat aatggttata gaggcaaggt tcaatttgca gttggactgc gagatcctaa
      481 tgtcgcggac aagtcaaaat caaatggttt tgaatctgac aacgacgcat ctgggtcttc
      541 aaacgaacca ttcacacaag ctgtattttc aaatgtgagt ttgttcggtc cctacacgac
      601 gacatcagat attgttgatc cacaaaaagg caatggacag ttccaagctg caatgcattt
      661 gcaacgtaac acacagttaa gatgttacaa ttccattttc gcgggatggc ctattggatt
      721 atttattgag aatggtggca agggtaatgc tcaagaaaac gcaattaacg gatcattaat
      781 tttgaacaac aacattatgg caggcatgaa ggtcaacttc aaggaagatg aaaaaggaac
      841 agcagatttt aattatacat attggaacga aagcagccgc aataatactt tacttacaaa
      901 taatattgac ttaggtttgg ttgacccatt caatattaca agtcctaagt tttacgcatc
      961 aaacagtaac ggagttatat ttggtaaagc aagtttcaac tcagacgacc tatcgggtat
     1021 gactaatgtt aactttgtgg gagcttttga tacagaagat tggacagatg aatggtgtaa
     1081 cttcgaccca cagaatacaa tttactaata agtttattaa taattaaaga gttatcgcat
     1141 cctcaagtaa ttgaaggggc gataactctt tttaaaataa ttatttatga aaaagcatta
     1201 tctaatacaa ctatttattg cgtgtctaat ttcaatatcg gtgtttgccg aatcagatat
     1261 aattacttta agtgatggta tatattttaa agatggcagt caaaaagaat tatacacagg
     1321 agattacaaa gaattgtatc ctgatggctc tttaaaatta gaaatgcgta ttaagaatgg
     1381 tcaaatcgaa ggtacttatg taacatactt tccaaatggc aatgtaaaag aagtgcgttc
     1441 ttatttagat ggtcaattac atggtacatg gcgcacatat aacagcgatg ctattcttac
     1501 tgcggaagcc atttactcac atggcaaaaa aaatggtaca tggagaatct gggatgatag
     1561 cggcaaactt cgctatcaaa tgaattacat caacaataaa aaaataggta tttggcaatc
     1621 ttggaacgaa aatggcataa ttgttcaaac aagaacctat aaataattac ttgtaaccca
     1681 tttttaatct ttacgtcata aacttgtaat atataaatcc ttactttgtg atataaaaaa
     1741 tatagaatga aaaagaaatt cggtgttacc ttaatggcat tattgtatgt aagttgcata
     1801 gccgtagcaa aaatagctac aaattcagtc gaaaaaaaga cgtctggtgt actatatggt
     1861 tatgtggtag atgcagaagc aggagaaccc attgcaggtg ctgttattga aatatgcaac
     1921 cagaaagttt tttcagattt agatggtaaa tttgaaatta acggtcttac atcaaacaaa
     1981 ttacaaatgc gagtaagtat gatttcgtac aacacacaaa cagtagaaat tgatttatca
     2041 aaacagtcta atgtttcaat aagtatttta caacaatgag tccccaaaaa cgaatattag
     2101 ttgtagatga tgaatctgac atctgcgaaa tacttaagtt caatttagaa aaaaataatt
     2161 ttatagtaga tgtagcatat tcagcggagg aggctctaga aaatgactta tcaacataca
     2221 atctatttat attagatgtc atgatgggaa aaatatcagg cttcaatttt gccaaaataa
     2281 cgcaacaaaa atttgaaact gcaaaaattc ctataatttt cataacagcc aaagacacca
     2341 ttgaagacaa atttgtaggc tttaaaatag gtgctgacga ctatatatcg aagccatttt
     2401 caatttcaga aacattggct agaattgaaa atattctcaa acggtatgat gagaacaata
     2461 agcatagcga tataaatttt aagcgaaata ttaccaccta caaaggaatt tacatagacg
     2521 aaaacagcaa aactgttact attgataata agcctgtaca actgacaaaa aaagaatacg
     2581 aactcctagt actattttta cataagccaa acaaaatttt ttctcgaaat gaattacttc
     2641 aattaatatg ggataatgac gtatacgtac ttgaaagaac tattgatgtc aatatcgcac
     2701 gtttaagaaa caaaatcacc ccttacgaag atatacttat cacaaaacaa ggatacggct
     2761 acaaatctaa tcttgaatag cattagctat ttactgcgcc tttggcatgt tatttcaata
     2821 taaaatcgta tttttgcgat ttttagaaat cattatgcaa tttacattac aacatacaga
     2881 tttaaaaagt caggcgcgag cgggattatt gcaaacagat cacggaacaa tagaaactcc
     2941 aatcttcatg ccggtcggaa ctgtcggttc tgtaaaagca gtacatcaaa gagagttgaa
     3001 tgatgatatc aaggcgcaaa ttattttagg taacacttat catttgtacc tgcgcccggg
     3061 aatcaatatt ttggaaaaag ccggaggttt acataagttt ataggttggg acaaacccat
     3121 tttaaccgat agcggcggtt ttcaagtatt ttctctttct gatacgcgca aattgaaaga
     3181 agaaggcgta actttccgtt cgcatattga tggttcaaag cacctgttta cacccgaaaa
     3241 tgttgtagat attgaacgta gtattggtgc cgacataata atggcattag atgaatgtac
     3301 accgggagat gcagattaca actacgcaaa gaaatcatta gaattaactc taaggtggtt
     3361 agatagagga gttaagcact tccgagaaac agaacctaaa tacggatacc atcaaagcta
     3421 cttccctatt gtacaaggct gtgtatatcc cgatttacgc aaaaaatcag cagaaataat
     3481 agcagaactg gatatggatg gaaatgccat tggcggttta gcagttggcg aacctgccga
     3541 aaaaatgtac gaaatgattg aagttgtaaa tgaaata
//
LOCUS       DAOPID010000160         3543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_36343_length_3543_cov_3.223337, whole genome shotgun sequence.
ACCESSION   DAOPID010000160 DAOPID010000000
VERSION     DAOPID010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36343_length_3543_cov_3.223337"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="PK641_05650"
     CDS             complement(<1..363)
                     /locus_tag="PK641_05650"
                     /inference="COORDINATES: protein motif:HMM:NF023325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3417 domain-containing protein"
                     /protein_id="HPG55866.1"
                     /translation="MKLQVCNANYPNWRPITVKFHVPKEFQKLMELSKNLWWVWNYEA
                     VELFSEMNPQLWIEVGRNPVLFLQRLTSEEYVELAKNADFMKRIDAVYANFCAYVSVK
                     PSKSIPSVAYFSMEYGLTD"
     gene            complement(593..668)
                     /locus_tag="PK641_05655"
     tRNA            complement(593..668)
                     /locus_tag="PK641_05655"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(631..633),aa:His,seq:gtg)
     gene            complement(734..1762)
                     /locus_tag="PK641_05660"
     CDS             complement(734..1762)
                     /locus_tag="PK641_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPG55867.1"
                     /translation="MVLLIVIIVLAISFDYINGFHDAANSIATIVSTRILTPFQAVLW
                     AAFFNFSAFFIPKYIIGEFGIADTVSKTVFPEFITMPIILSGIIAAIIWNLLTWRLGI
                     PSSSSHTLIGGFAGAAIAATGFQAIHTEVIIIIAIFIFIAPLVGMIGGNLMMILTLHI
                     VRKAKPHKADKWFKRAQLLSSALLSIGHGLNDSQKIMGIIAAALISFSSTHTNVPHWL
                     QLSDIHDIHDWVPIACFTAIAAGTMSGGWRIMKTMGDKITKLTPVEGFCAETSGAITL
                     FLTEVLKVPVSTTHVITGSIIGVGSVKRLSAVRWGVTVDLLVAWILTIPVSAVLAMSV
                     YFIVSLFL"
     gene            complement(1765..2415)
                     /locus_tag="PK641_05665"
     CDS             complement(1765..2415)
                     /locus_tag="PK641_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HPG55868.1"
                     /translation="MELNSFFSFIIPKENKFFPLIIEVGDKCAEASEYLVQFMNEPDK
                     AKAKEIYLKIKQCEIVGDTLIDNIFNELNDTFITPFDREDIHDLCETLDDVIDYITSS
                     AKRMVLFQPHFVPELTKELAENIRQGCLSIQIAMRELKTISKKPALALEQCGILHDLE
                     HQADDMYERFVTDLFKEETNSIELIKNKEIMQELEKTTDKAKVVGKILKTIIVKYA"
     gene            complement(2473..3336)
                     /locus_tag="PK641_05670"
     CDS             complement(2473..3336)
                     /locus_tag="PK641_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HPG55869.1"
                     /translation="MVEIGKTNALEVVKKVDFGVYLDGDEFGEILLPLRYVPAECNIG
                     EFLNVFIYTDSEDRVIATTETPLAQVGDFAYLQVKDTSSVGAFLDWGLMKDLLVPFSE
                     QKADLNIGNKVFVYVYLDEKTKRIVASAKWEKFIEKDIVDLSEGTEVSLKIAAKTELG
                     YKAVIDGKFMGLLYKDEVFRSIQIGDTVTGFIKHIRPDAKIDLSLTIGGYGKIKNETE
                     RILEKLRESGGFLPTTDKSPAEFIYQTYGISKKTYKQAIGDLYKRCCVLLEQDGVRLN
                     TTKQKNIKNNL"
     gene            complement(3349..>3543)
                     /locus_tag="PK641_05675"
     CDS             complement(3349..>3543)
                     /locus_tag="PK641_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007758067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HPG55870.1"
                     /translation="IRLYKACWVESDDAAELENFKKRKYQFMAKDKEGRDVFLADSGY
                     VLQMAQEDFKKIRFHFSSEF"
BASE COUNT         1168 a          687 c          488 g         1200 t
ORIGIN      
        1 atctgtcaaa ccatattcca tgctgaaata tgctacagaa ggaatagatt tactaggttt
       61 tactgatacg tacgcacaaa aatttgcata aactgcatct atacgtttca taaaatcagc
      121 gttctttgcc aactccacat attcttcact tgtcagcctt tgcaaaaaca aaaccggatt
      181 tctaccaact tcgatccata attgaggatt catttcgctg aacaattcta cggcttcata
      241 attccaaacc caccataggt tctttgaaag ctccattaat ttttgaaatt cctttgggac
      301 gtgaaattta acagtaatag gtctccagtt aggataatta gcattgcata cttgtaattt
      361 cataattcta taataagagt taataaatat cattaaaccc aagtagtaaa atattacata
      421 cttgagcgga gcacaaagtt agtttttttg ttttatctaa caagagtttt taaaaaaaag
      481 ctaactgttt gttgatatct ttttcctgat tttcagcata tttagtgtgc aatcgtttga
      541 acactgcata aaaaaaagtg cttctttttt aaaaaagcac tttatatatt aagggtgaaa
      601 gatgggactc gaacccacga cattcggaac cacaatccga cgctctaacc aactgagcta
      661 caatcaccat ctatgtcaaa tccctttgac gacgcaaagg taacacattt ttttatttca
      721 acaacaaaaa aaattacaaa aacaatgata caataaaata aacagacata gccaagactg
      781 cactaacagg tattgtcaat atccatgcta caagtaaatc aacagttacg ccccatctca
      841 cagcagacaa tcttttaaca gaacccacac ctataatact tccggttatt acatgagtgg
      901 tactaaccgg cacctttaag acttcagtta aaaataaagt tattgcacct gaagtttctg
      961 cacaaaagcc ctctaccggt gttaattttg ttattttatc acccattgtc ttcataatac
     1021 gccacccacc tgacattgtt ccggcagcta ttgcagtaaa acatgcaata ggtacccaat
     1081 catgaatgtc gtggatatca gataactgca accaatgagg tacatttgta tgtgttgacg
     1141 agaaactaat taatgctgct gcaattattc ccattatttt ctgtgaatca tttagtccat
     1201 gtcctatgct taataaagca gatgataata attgagcacg tttaaaccac ttatctgctt
     1261 tatgcggttt cgctttacgc acaatatgta aggttaatat catcattaaa ttacctccta
     1321 tcattcctac aagtggggca ataaaaataa aaatagcaat tataattatg acttctgtat
     1381 gaatagcttg aaatcctgtc gctgctatag ctgcaccggc aaaaccgcct attagtgtat
     1441 gagaagatga tgatggtatt cctaaacgcc atgtaagcaa attccaaatt attgcagcaa
     1501 taatacccga taaaataata ggcattgtaa tgaactcagg aaaaactgtt tttgacacag
     1561 tatctgctat accaaattca cctataatat attttggaat aaagaaagct gaaaaattaa
     1621 aaaatgcagc ccataaaacg gcttgaaaag gagtcaaaat acgagtagaa acaattgtag
     1681 ctattgaatt agccgcatca tggaatccgt tgatataatc aaatgagatt gctaaaacaa
     1741 ttattacgat taaaagaacc atgtctatgc gtatttaacg ataattgttt taagaatttt
     1801 tccaacaacc ttcgctttat cggtagtctt ttccaattcc tgcattattt ctttgttttt
     1861 aattaactcg atactatttg tttcttcttt aaacaaatct gtaacaaaac gctcgtacat
     1921 atcatcagcc tgatgctcca aatcgtgaag tatgccacat tgttctaaag ctaacgcagg
     1981 tttctttgaa attgttttta attcgcgcat tgcaatttga attgacaaac aaccttgtct
     2041 tatattttca gctaattcct ttgtcagttc aggaacaaaa tgaggttgaa ataataccat
     2101 acgttttgca gatgatgtta tgtagtcaat tacatcatct aaagtttcac ataaatcgtg
     2161 aatatcttct ctatcaaatg gagtaataaa cgtatcatta agttcattaa agatattgtc
     2221 gattaatgta tctccaacaa tctcacattg tttaattttt agataaattt ccttagcttt
     2281 agccttatca ggctcattca taaactgcac taaatattca gatgcttcag cacatttgtc
     2341 gccaacctct ataataagag ggaaaaactt attttctttt ggtataataa aagaaaaaaa
     2401 cgaatttaat tccatgttta aggttaaaaa gatgttgcaa aagtaattaa ataatagtat
     2461 tcagatgcta ttttataaat tatttttgat gtttttttgc ttggtagtat ttaagcgtac
     2521 tccatcttgt tctaagagca cacaacacct tttgtataaa tcgccgatag cttgtttata
     2581 cgtttttttg ctgatgccgt aagtctgata tataaattct gccgggcttt tgtctgttgt
     2641 tggcaaaaag cctcctgatt cacgtaattt ttcgagtata cgttcggttt cgttttttat
     2701 cttgccatat ccgcctattg tcaatgacaa atctattttt gcatcaggtc tgatgtgttt
     2761 aataaatcct gttactgtat cccctatctg tattgatcta aaaacctcat ctttatataa
     2821 aagtcccata aattttccat caataacagc tttataacct aattctgttt tagctgctat
     2881 ttttagagaa acttctgttc cttcagataa atcaacaata tccttctcta taaatttttc
     2941 ccattttgct gatgctacaa tacgctttgt tttttcatct agatagacat atacaaaaac
     3001 tttatttcca atattcaaat cggctttttg ctcactaaaa ggaactaaca aatccttcat
     3061 caatccccaa tccaagaaag cccctactga tgatgtatct ttaacttgta gatatgcaaa
     3121 atcgccaact tgtgccaatg gcgtttctgt tgttgcaata actctatctt cagaatcagt
     3181 atatatgaaa acattcaaaa attcaccaat attgcattct gcaggcacat atcgcaatgg
     3241 tagtaatatc tcaccaaatt cgtcgccatc taaatacact ccgaaatcta cttttttaac
     3301 aacttcaaga gcgtttgttt ttcctatctc taccatttga ttttctcctt aaaattcact
     3361 tgaaaaatgg aatcttattt ttttaaaatc ttcttgagcc atttgtaata catatccgga
     3421 atcggctaag aaaacatccc taccctcctt atctttagcc ataaactgat atttacgctt
     3481 cttaaaattt tcaagttcag ctgcatcatc actttctacc caacaagcct tatataatcg
     3541 tat
//
LOCUS       DAOPID010000161         3519 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_36677_length_3519_cov_3.717379, whole genome shotgun sequence.
ACCESSION   DAOPID010000161 DAOPID010000000
VERSION     DAOPID010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36677_length_3519_cov_3.717379"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..1068)
                     /locus_tag="PK641_05680"
     CDS             complement(127..1068)
                     /locus_tag="PK641_05680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPG55871.1"
                     /translation="MKSVSVVIPTYNRAMYIERSVRSVLQQTYQNLEIIVVDDGSTDD
                     TLQILQKLQHEDHRLKILQQEKNLGAQAARNTGIRAATALYIAFLDSDDEWLASKLEK
                     QLSLIDQSNDKIGVVYAGFMWVYSDGRPSKEQKPRFKGKIYNDALRGWIADTNTLVVR
                     KDILYKIGLLNEGIRAYQEWDLCIKLSRYTEFDFVNEPLAIYHLHDQPTISKNLVNDA
                     LGYLDIIMAHKNEIVTELGNDALAEHFLSASLRFTRIDDFKTARKLVSESIRISHLKM
                     RSKLASYWILFHLNPSSYKALTSILGKMRSFYRNMEF"
     gene            complement(1090..1926)
                     /locus_tag="PK641_05685"
     CDS             complement(1090..1926)
                     /locus_tag="PK641_05685"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPG55872.1"
                     /translation="MDKYWNNPLLWEQYGRDEYYSERASFVKNIIPDDVNSLLDVGAG
                     KGEIINNLTENIDTVMGLDISEEALKYLNCKAVLGSIENLPFDDKSFDLVMCMEVLEH
                     LPIKSLSLGVHELQRVAKNYLLISVPFNENIDARKVRCPRCKMIFNIDTHFRNFQTER
                     ELCQLFPDFQLNKKWLCGPSYRRPSRVQLFIQQKILNIYYPLDHYFVCIFCGAKYSEF
                     ESITSNERTSFAKNRFGLLRSLSRLRIFEMLFKLFSFLKKNEACWMFVLLRRNEIIKI
                     NH"
     gene            complement(1931..3334)
                     /locus_tag="PK641_05690"
     CDS             complement(1931..3334)
                     /locus_tag="PK641_05690"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HPG55873.1"
                     /translation="MTEIAARTAQPIIFVVLARLLAPTDFGVLATAMIVISFVQMFWD
                     AGLSKALIQTKEVPENAAHVVFWTNLTLGIIIYTLLFAAAPYIAFFFKSPVTEPVLRV
                     LGIQIIIASLASVQQALFVRDLDFRGLFWIKLLTVFIPALFSIPLALLGYGIWSLVTG
                     TLAGQFFNLLLLWMRSPWRPELKYDLKLAKKLMTFGIWILLGSLGSWLIMWVDNLIVG
                     KFLGVYELGLYRIGWMSVTLIYGLVLNPIMVVLYPTISRMQDNLPMLKDMIYKMNKII
                     ISIALPIGIGSLFIGKNAAVLLFGDKWLGLGFVFSILAFREAISWACGINSEVYKAIG
                     KPDIIAKIEYALLLIIIPIYFFSVKFGLEIFLFARLGVTVIGLFVHMKIFQHILNLSR
                     DYLWQHMKMAMLSCVSMAIVLCISKELCSIKRLPGIVELFMLSAIGICAYAATYWLLD
                     RNFLIYAFKVVKRKAIA"
BASE COUNT         1134 a          716 c          636 g         1033 t
ORIGIN      
        1 gtgtatgagt ctgaaatgcc ctgaaggtaa ctacgtctgc agaaatatgt cccagtgaga
       61 agattttgtg gcgtctgaaa tgtagtattt tgagacgttg aacggtatgt cgcagaaatc
      121 atatttttaa aattccatat tacgataaaa agaccgcatt tttccaagaa tagaggtaag
      181 agccttatat gagctcggat tgagatgaaa tagaatccaa taggatgcca atttagatct
      241 catcttcaaa tgagaaattc taattgattc tgaaaccagc tttctagctg ttttgaaatc
      301 atctatacgc gtaaaccgaa gcgaagcgga aagaaaatgc tcggcaagag catcattacc
      361 gagttcggtt acaatctcat tcttatgagc cattattata tcaagatatc caagagcatc
      421 attcacgaga ttttttgaaa tcgtcggctg gtcatgcaga tgatagattg ccaatggttc
      481 gtttacaaaa tcaaactcag tgtatcgtga aagtttaata caaaggtccc attcctgata
      541 agcgcgaatg ccttcgttaa gtaaaccaat tttataaaga atgtcttttc gcacaactaa
      601 ggtattcgta tcagcaatcc atcctctgag ggcatcgttg taaattttac ccttgaagcg
      661 gggtttttgt tctttcgaag gcctcccatc agaataaacc cacatgaagc cagcatatac
      721 aacaccaatt ttgtcatttg actgatcaat tagcgagagt tgcttttcca gtttactagc
      781 caaccattcg tcgtctgaat caaggaatgc tatatagaga gccgtagctg cacgtattcc
      841 tgtgttgcgt gcagcttgag ctcctaaatt tttctcttgt tgaagaatct tgagacgatg
      901 gtcttcatgc tgtaattttt gaagtatttg taaggtatca tctgtcgatc catcgtcaac
      961 gacaattatt tccagattct gataagtctg ctgaagtaca ctacgaactg atctttcgat
     1021 gtacatagct cggttatagg ttgggatgac gacggatacg gatttcacaa aaactttaca
     1081 atatcaaatc taatgattta tttttatgat ttcattcctt cttagtagaa caaacatcca
     1141 acatgcttca ttcttcttca agaacgaaaa taatttaaat agcatttcaa aaattcgcag
     1201 tcgagataat gatcggagta aaccgaaccg gttttttgca aatgaagttc tctcatttga
     1261 tgttattgat tcaaattcgc tatatttggc gccgcagaat atacaaacaa aataatggtc
     1321 taacggataa taaatgttca gtatcttttg ctgaatgaat aattgaactc gtgagggtct
     1381 tctatatgac gggccacata accatttttt gtttaactga aagtcgggga aaagctgaca
     1441 taattctctt tcggtctgaa aatttctgaa atgcgtatct atgttaaaaa tcattttaca
     1501 acgcggacat ctgacttttc gtgcatcaat attttcgtta aaaggtacgc tgatcagtaa
     1561 ataatttttt gcgaccctct gaagttcatg aactccaaga ctcagagatt taatcggtag
     1621 gtgttctaag acttccatgc acattacgag gtcgaagctc ttgtcgtcaa aaggcaaatt
     1681 ttcaatactt cccaatactg ctttacaatt tagatatttc aacgcttcct ctgaaatatc
     1741 taatcccata actgtatcaa tgttttcagt gagattgttt atgatttcac ctttgccggc
     1801 gccaacgtct aagagggagt tcacatcatc cggtattata ttcttcacaa aagatgcacg
     1861 ttcagagtaa tattcatctc ttccgtattg ttcccatagc aacggattat tccaatattt
     1921 atccaaatac ttatgcaatt gctttccttt tcacgacctt aaaggcataa atcaaaaaat
     1981 ttctatcaag aagccagtat gttgcagcat aagcgcaaat gccaattgcc gatagcatga
     2041 atagttcaac aattcccggc aacctcttaa tactgcaaag ctcctttgat atacacaaca
     2101 caattgccat tgagacgcaa gacaacatcg ccatcttcat atgttgccat aaataatcgc
     2161 gcgataagtt taaaatatgt tgaaaaatct tcatatgaac aaataatcca ataacagtaa
     2221 cacccaatct tgcgaataaa aaaatctcta gtccaaattt tactgaaaaa aagtaaatag
     2281 gaatgattat taataataat gcatattcta tcttcgcaat tatatctggt ttcccgattg
     2341 ctttgtacac ctcactattg attccgcaag cccaagaaat tgcctcacga aatgcgagga
     2401 tgctaaatac aaatcctaat cccaaccact tatcgccaaa taagagaact gcggcgtttt
     2461 tccctatgaa taaagatccg atacctatag gtaatgcaat ggaaataata attttgttca
     2521 ttttatagat catatctttc agcataggaa gattgtcttg catacgtgaa attgttggat
     2581 aaagcacaac catgatagga ttaagaacaa ggccataaat gagtgtcacg gacatccatc
     2641 ctatcctgta taatcccagt tcgtatacac ctaaaaactt tccaacaatc aaattatcaa
     2701 cccacatgat caaccaagaa ccaagactac ctaacaatat ccatataccg aaagtcatca
     2761 atttttttgc caatttcaag tcatatttaa gttctggtcg ccatgggctt ctcatccaaa
     2821 gcaacaagag gttaaagaac tgcccagcca gggttccggt gacaagtgac caaatgccgt
     2881 agcccaataa tgcaagcgga atagaaaaca aagccgggat gaataccgtg agcagcttga
     2941 tccagaaaag ccctcgaaaa tcaagatcac ggacaaataa ggcttgttgt acggacgcaa
     3001 gagacgcgat gatgatctgg atgcccaaga cgcgcaatac cggttcggta acgggactct
     3061 taaagaaaaa ggcaatgtac ggggcagcgg caaataaaag ggtataaatt ataattccca
     3121 gtgtaaggtt ggtccaaaat accacatgag ctgcattttc aggaacttcc tttgtttgta
     3181 tcaatgcctt gctcaggccc gcatcccaaa acatttgtac aaagcttatg acgatcatag
     3241 ctgttgctaa aacaccaaaa tcagtaggcg caagtaaacg tgcaaggaca acgaagatga
     3301 tcggttgggc tgttctagct gcaatttccg tcaaggccga ccacttcaaa gatccgacag
     3361 cttttgttgt aatgttctga ttcgaatagt tcacgagatt atccctacaa cgacacataa
     3421 cgcagtctct ccaatgccgt tagttttcgt ttccgatgag agagataagc ccgatagttt
     3481 ttcatctgta tccatgagac aagctcaatg agatcgacg
//
LOCUS       DAOPID010000162         3514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_36746_length_3514_cov_3.889853, whole genome shotgun sequence.
ACCESSION   DAOPID010000162 DAOPID010000000
VERSION     DAOPID010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36746_length_3514_cov_3.889853"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..405
                     /locus_tag="PK641_05695"
     CDS             40..405
                     /locus_tag="PK641_05695"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPG55874.1"
                     /translation="MDLKQLGTTIMNVRRKLAKTQEAVAADLDISLTAYSKIERGETN
                     LQFNRLSQIADYFNMEPWQLLCPTYNFDFNEFKSEFDSLKLEIKTLRDDIAKYPATNT
                     HIYKSESRLVNQSKQSSKK"
     gene            complement(598..1041)
                     /locus_tag="PK641_05700"
     CDS             complement(598..1041)
                     /locus_tag="PK641_05700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HPG55875.1"
                     /translation="MTRQETIDKAVAYFTEGYACSQSVVLAFAEQVNLDPTIAKRISS
                     TFGGGMGRLRQKCGALTGAFMILGLKEGYDSPSDMDGKLCVYKKVRELDKQVEKEFGT
                     TNCNELLIKYSVPQHVKERSHHKDICVRVVADAAGKLYDMINAND"
     gene            complement(1127..3418)
                     /locus_tag="PK641_05705"
     CDS             complement(1127..3418)
                     /locus_tag="PK641_05705"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPG55876.1"
                     /translation="MRYKSFLLCVAFSYLFAFSQVVNAASIVQQDDDILLSFDNQSSL
                     NVIIQKSTGKFGTYETIGQTYLSSFIDNNISGNPYSYYYKILTPDYEVLCTLSLETEL
                     FGPNVYIYSPSDDMSQVGKEINTIHDEMQYSQFGTNRYAFYFKPGNYADAGTLKIAYY
                     MHFGGLGKTPYEVGLSNIIPPAPLLDNNGTCTFWRSLENFSVLSEPESTKEDYFWWGV
                     SQAAPIRRVYSERKTIFDYYGGQASGGFAADCYFDDAAGSYSQQQWYTRNSYIEKGSS
                     GINKGGWNNAYQGVEFGSSVYMPNHSDNWEINKNSFGNVSRVETTPIIREKPFLFIGD
                     DGRYKVFKPALRENSIGTSYSQEDMGNGEILDLLNDFYIAKPGVTASTLNIQLSAGKH
                     LFFTPGIYELEEPIYITKENAIILGTGYATLITAESNNTAAIVASDVNGITIASLLFD
                     TYYHTKYLLQIGPDGSDADHSSNPSLLADLFFRVGGVKTTNVNADVALEINSSNVIGD
                     HFWIWRADHGNGVGWTKNTSKNGLIVNGDNVTLYGLFNEHFQEYQTLWNGEYGKVYFY
                     QSETPYDPTSQSIYMSHEGTKNGYASYKVSDLVEHHYACMMGIYDVFIRTGSATIAID
                     NAIEIPRTKDVKIHHACNVGLSSRGGINYIVNESVKSTYGTTGAKRFYILDYCETGID
                     ENIALNNAEISIYPNPVVDVLNIRSKNNKNINKISILDFNGKVLKSQDYSESIDMSAF
                     ESGLYFISIETEGGSGIAKIIKQ"
BASE COUNT         1154 a          656 c          490 g         1214 t
ORIGIN      
        1 tgtataaata tttgttttcc ataatgtatt tttgtcaata tggatttaaa gcaattaggt
       61 acaacaataa tgaatgttcg ccggaagtta gcgaaaactc aagaagccgt ggctgcagac
      121 ctcgacatct cgcttactgc atactcaaaa attgaacgag gcgaaacaaa tctacaattc
      181 aatagattgt cgcaaatagc cgattatttt aatatggaac cttggcaatt gctttgccct
      241 acttataatt ttgattttaa tgaatttaaa tcagagtttg attccctaaa gcttgagatt
      301 aaaacattac gcgatgatat agctaaatat cccgcaacca acactcatat atataaatca
      361 gagtcgcgtc ttgttaatca aagtaagcaa tcttcaaaga aatagtttat ctttaagttc
      421 tatttttact atcacattta taaagacaaa aggtaactcc tgataaagaa gttacctttt
      481 taattaaaat ataagattaa ggaattatac ttttggaaaa taatatactt aaatattcag
      541 aaggataaat atcagcatta aattaactgc gaacatagta taattattgc atttaattta
      601 atcattcgca tttatcatat cgtataattt tccggcagca tccgctacaa ctcttacaca
      661 tatatctttg tggtgactgc gctcttttac gtgctgcgga acagaatatt tgatcaataa
      721 ttcgttgcaa tttgttgtgc cgaattcttt ttctacttgt ttgtctagct cacgcacctt
      781 tttataaacg cacaattttc catccatgtc agatggagaa tcatatcctt cttttaatcc
      841 caatatcata aaagctcctg tcagtgcacc gcatttttgt cttaaacgcc ccattccacc
      901 accaaaagta gaagatatcc gtttggctat agttggatct aaattaactt gttcggcaaa
      961 tgccaatacg acagattgag aacatgcata cccttctgta aaataagcaa ctgctttatc
     1021 tatggtttct tgtcgtgtca taataatttt ctttatatat attggatgca aatatataaa
     1081 ttaaattttt atgtcaatcg atcaattagt caataacatg ttttacttat tgtttgataa
     1141 tcttagcaat tccactgccg ccttctgttt ctatactaat aaaatatagt ccgctttcaa
     1201 aagcactcat atcaatggat tctgaataat cctgagattt caatactttg ccgttgaaat
     1261 ctaatattga aatcttgttt atgttcttat tgttcttact tcttatattt aatacatcta
     1321 caacaggatt cggatatatt gagatctctg cattatttaa agcaatattt tcatcaattc
     1381 ctgtttcaca ataatcaaga atgtaaaatc tttttgctcc tgtagttcca taagtgcttt
     1441 taactgattc atttactata taattaatcc ctccgcgact cgaaagacct acattacatg
     1501 cgtgatgaat ttttacatcc ttagtacgtg gaatttctat ggcattatca atcgcaatag
     1561 ttgcacttcc tgttctaata aacacatcat aaatgcccat catacaagca taatgatgtt
     1621 ccaccaaatc cgatacttta tatgaagcat atccattttt cgttccttca tgactcatat
     1681 aaatactttg agatgtcgga tcgtaaggag tttcactttg atagaaatag actttaccat
     1741 attctccatt ccaaagagtt tgatattctt gaaaatgctc attgaataaa ccatataatg
     1801 taacattatc accatttaca attaatccgt ttttagatgt attttttgtc caacccactc
     1861 cattaccatg gtctgctctc catatccaaa aatggtcacc tattacgtta gaactattta
     1921 tttctaaagc tacgtctgca ttaacattag tagttttaac acctccaact ctaaaaaaca
     1981 aatcagccaa aagactcgga ttagatgaat gatctgcatc tgagccatca ggtcctattt
     2041 gtaataaata ttttgtatga taatatgtat caaataataa acttgcaatt gttataccat
     2101 ttacatctga tgcaacaata gctgccgtat tatttgattc tgccgtaatt aatgttgcat
     2161 aacctgtacc taaaatgatg gcattttctt ttgttatata aattggctct tcaagttcat
     2221 aaatacctgg cgtaaaaaac agatgtttac ctgccgatag ttgaatattt aatgtagatg
     2281 cagtaacacc tggctttgca atatagaaat cattcaacaa atcaagtatt tcgccattcc
     2341 ccatatcttc ttgcgaatat gaagtaccga ttgagttttc gcgtaaagca ggtttaaaca
     2401 ctttataacg gccatcatca ccaataaata agaatggttt ttctctgata ataggagtag
     2461 tttctaccct acttacattt ccaaatgaat ttttattaat ttcccaatta tcgctatgat
     2521 taggcatata aacgctagag ccaaattcaa cgccctgata agcattattc caacctccct
     2581 tattaatacc ggatgaacct ttttctatat atgaattacg tgtataccat tgttgctgag
     2641 aataggaacc tgccgcatca tcaaaataac aatcagcagc aaaaccacca ctggcttgac
     2701 ctccataata atcaaatatt gttttacgtt ctgaatatac gcgacgtatt ggagctgctt
     2761 gtgatactcc ccaccagaaa taatcttctt tcgttgattc cggttctgat aaaaccgaga
     2821 agttttctaa agaacgccaa aaagtacatg ttccattatt atctaacaat ggagccggtg
     2881 gtattatatt actcaagcct acttcgtacg gggttttacc caatccaccg aaatgcatat
     2941 aatatgcaat tttcagagtt cccgcatcag cataattgcc cggtttgaaa tagaaagcat
     3001 atcgatttgt accgaattgg ctatactgca tttcatcatg tatggtatta atttcctttc
     3061 caacttgaga catatcatca cttggagaat agatatacac atttggacca aataattctg
     3121 tttctaaact cagtgtacaa agtacttcat aatcaggagt taatatctta tagtaataac
     3181 tatatggatt accgcttatg ttattatcaa taaatgaact taaataagtc tgccctatgg
     3241 tttcataagt gccgaatttt cctgtcgatt tttgaattat aacatttaac gaagattgat
     3301 tatcaaaaga caacaaaatg tcatcatctt gttggacaat acttgcagca ttaacaactt
     3361 gggaaaacgc aaacagatag gaaaatgcca cacaaagtaa aaaagatttg tatctcataa
     3421 ttgattttta aaattgttac acaataggtg tatacatatc gtaactattt atcagatgca
     3481 aagttatctc tttaaattga tatttctatt tttg
//
LOCUS       DAOPID010000163         3474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_37353_length_3474_cov_4.577654, whole genome shotgun sequence.
ACCESSION   DAOPID010000163 DAOPID010000000
VERSION     DAOPID010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37353_length_3474_cov_4.577654"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /gene="mnmE"
                     /locus_tag="PK641_05710"
     CDS             complement(<1..1314)
                     /gene="mnmE"
                     /locus_tag="PK641_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007766255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HPG55877.1"
                     /translation="MIQLDTTICAISTAPGVGGIAVIRVSGKQAICLSDKIFKAKKSL
                     LEQSSDMAVFGRIVDSEAKVIDEVIVTVFRAPHSFTGEDVVEISCHGSLYIQQEIVRL
                     LLAQGCVMAGPGEFTQRAFLNGKMDLSQAEAVADLIASTSAASHRVAINQMRGGFSSE
                     LALLRDKLLEFTSLIELELDFGEEDVVFADRSRLKSLSQVIDAKIDSLSKSFSAGNAI
                     KNGVPVAIVGETNAGKSTLLNVLLNEERAIVSDIHGTTRDLVEDTSVLSGIMFRFIDT
                     AGIRETDDDIEKLGIKRSYKAIEQASIVIWMIDATQVNEHIDWMAERIIPRAQGKKLL
                     LVFNKVDKVSESEKNVLEDLFSNVEAQSIFISAKYKQNISELIKLIVESSHLPEISSE
                     DVIVTNLRHYEALCKALEAIRRVQDGLDSKLSGDLLSRDLHDCLDA"
     gene            1494..2057
                     /gene="efp"
                     /locus_tag="PK641_05715"
     CDS             1494..2057
                     /gene="efp"
                     /locus_tag="PK641_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPG55878.1"
                     /translation="MATTADFKTGMCIEHNGALYYVVNFLHVKPGKGNTFVRTKLKNV
                     KTGLVLEERFNAGVRINVARVERRPMQFLYKDDMGYNLMNTETFEQITIPEGGINGAA
                     FMKEGEMIEAVFHADTESLLYADMPASVILEVTYTEPGIKGDTATNTLKPATVETGAT
                     VRVPLFINTGDKIKVNTQDGSYVERAK"
     gene            2477..>3474
                     /locus_tag="PK641_05720"
     CDS             2477..>3474
                     /locus_tag="PK641_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55879.1"
                     /translation="MAKRIFNILIVFCIAAVNLFAQSGINVGTPNLSFEQGNFNNWTR
                     ELGNFRVLASDYNSYEYYNWTTVTNSGQRIELQSSMNTNDPVFACSNFYTNPIGYSAA
                     RVGRPMYSEGMPNGGYSCTQRWNNYRGAAAERLTYTFTVTKQTTLLYFQFAMVLNNPG
                     TATGKHIGQQYPTFGINITITDPVSGLVSKPQCTSIELHPSDDPANEKYLKRYTSQCN
                     NSIAGSDLINFQYLPWTIGAVNLIDFIGQTVTIEIYNHDCLVICDGYPVAGGHSAYGY
                     FTAETRSLGFNIRNCEGEDAVITAPSGFSKYTRSRSDGLPITYQTAGDSSAIVVDQ"
BASE COUNT         1144 a          649 c          614 g         1067 t
ORIGIN      
        1 ggcgtcgagg caatcatgca agtcgcgtga taataaatcg cctgacagtt tggagtcgag
       61 tccgtcttga actcggcgga tagcttcgag ggctttacat aaagcttcgt aatgacgtag
      121 gtttgtaaca attacatctt ctgatgatat ttcgggcaaa tgagatgatt ctacaatcag
      181 tttgattaat tcagatatgt tttgtttgta ttttgcagat ataaagatac tttgagcttc
      241 aacgttagaa aataagtctt caagaacatt tttttcggat tcggatacct tatctacctt
      301 attaaacacc aacagaagct ttttgccttg agcgcgaggt ataatacgct ctgccatcca
      361 atcgatatgc tcgtttactt gagttgcatc aatcatccaa attactatgg aggcttgttc
      421 aatagcctta taactacgtt ttatacctaa tttttctata tcatcatcgg tttcgcgaat
      481 acctgctgta tcaataaaac ggaacatgat gcccgacaaa acagaagtat cctcaactaa
      541 atcgcgagtt gtaccatgta tgtcagatac aattgcacgt tcttcattca ataaaacatt
      601 tagaagggtc gatttcccgg catttgtttc gccgacaata gcaacaggca caccattttt
      661 aatagcattc cctgctgaaa atgattttga taaggagtca atttttgcat caatcacttg
      721 tgatagcgat tttaaacgcg atctgtctgc aaaaacaaca tcttcttcgc caaagtctaa
      781 ttccaattct attaatgaag taaactccaa tagtttatca cgcaataatg ccaattctga
      841 cgaaaaaccg ccacgcattt gattaattgc aacacgatga gatgctgcag atgttgaagc
      901 aataaggtca gctaccgctt cggcctgcga caaatccatc ttgccattta aaaatgcacg
      961 ttgggtaaat tcaccgggac ctgccataac acaaccttga gccaacaata atcgtactat
     1021 ttcttgctgt atatatagtg agccgtgaca tgatatttca actacatctt cacctgtaaa
     1081 agaatgaggc gctctaaata cagtaactat gacttcatca attactttag cttctgaatc
     1141 aactatacgg ccaaagactg ccatatcgga gctttgctct aataaagatt tttttgcttt
     1201 aaatatctta tctgataaac aaatggcttg tttacctgaa acgcggatta cggcaattcc
     1261 ccctactccc ggagcagttg aaatagcgca tattgttgta tctaattgaa tcatagccac
     1321 aaaattacta cattttctgt catcaccgaa taaataaaca atataaacgt tagaatagga
     1381 tgatttagcc ggtgaaaatt gaaaaacaat tacgtatgac caaatatgtc agatagtatt
     1441 gaattttttt catatctttg cagcctaaaa acaaacacat caaattaata tttatggcaa
     1501 ctacagctga ttttaaaaca ggcatgtgca ttgaacataa tggggctttg tattatgtcg
     1561 ttaattttct acatgtaaaa cccggaaaag gaaacacctt tgtacgcaca aaactaaaaa
     1621 acgtaaaaac cggattggta ttggaagaac gctttaatgc aggtgttcgc atcaacgttg
     1681 cacgcgttga acgcagacct atgcaatttc tatataaaga cgacatggga tataacttaa
     1741 tgaatactga aacatttgaa caaatcacta ttcctgaagg tggtattaat ggtgcagcat
     1801 ttatgaaaga aggagaaatg attgaagctg tattccacgc tgatacagaa tcattattat
     1861 atgcagatat gccggcatca gtaattttgg aagttacata cacagaaccg ggtattaaag
     1921 gtgatactgc aactaacaca cttaaacctg caactgttga aacaggagct actgttcgtg
     1981 ttcctttgtt tataaataca ggagataaaa tcaaagtcaa tacccaagat ggttcgtacg
     2041 tagagcgtgc aaaataaggt attaatattt tatactttaa tggagatgtt ttttttaaac
     2101 atctccattt ttattttaac agtttatata cgacttgtta tttttagtac aaaaatacaa
     2161 atatcattct atttcaataa gataagtatt tgtaatacta attaacatca caagtgatat
     2221 ctgcagccat agcaaaaact cgtttttaaa ttctcttttt agtaaaaaaa tacttaactt
     2281 cgcaaagatg ttcgctagaa ttctacgact gatgcctttg tatgaaaaac cgaaattgcg
     2341 tggctgtaac tttgaccctt taggtttaac cgggtcatca ctcgtcataa cgataaacgt
     2401 ttcgcaaact ttgattacat acaatttctt gtagaatgta aaccatagtt aatatcaaat
     2461 gagggaaaac tcaaatatgg cgaaacgcat tttcaatata ttaatagtgt tctgcatcgc
     2521 agcggtgaat ctatttgctc aatccggcat aaatgtcggc acacctaact tgtcttttga
     2581 gcaaggtaac ttcaataatt ggacacgaga attaggaaac ttcagagtac ttgcaagtga
     2641 ttataatagt tacgaatact acaattggac tacggtaaca aattcgggac agagaataga
     2701 gttgcagagt tcaatgaaca ccaacgaccc tgtttttgca tgtagtaatt tttataccaa
     2761 tcctatcgga tattcagctg cacgtgtagg tcgtccaatg tattcagaag gcatgccaaa
     2821 tggtggatat agctgcacac aaagatggaa taattacaga ggtgcagcag ctgaaagatt
     2881 gacatataca ttcactgtaa ctaaacaaac taccttgctg tattttcagt ttgcaatggt
     2941 tttaaataat ccaggaacag caacaggaaa acatattggt cagcaatatc ctacatttgg
     3001 aatcaatatt acaataacag atcctgtatc aggtttagta tcaaaaccac aatgtaccag
     3061 tatcgaatta catccatctg atgatccggc taatgaaaaa taccttaaaa gatatacttc
     3121 ccaatgtaat aatagtatag ccggctctga tttaatcaat tttcaatatc tgccttggac
     3181 aatcggagct gtaaatctaa tagattttat aggtcagact gttactatcg aaatttacaa
     3241 ccacgactgt ttggttattt gcgatggtta tcctgttgcc ggtggtcact ctgcgtatgg
     3301 ttattttaca gctgagacac gctctcttgg ttttaacata cgtaattgtg agggtgagga
     3361 tgctgtaatt actgctccat ccggattttc aaaatacact aggagtagat cagatggact
     3421 acctattaca tatcaaacag ccggcgatag ttctgcaatt gttgtagacc aaag
//
LOCUS       DAOPID010000164         3471 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_37401_length_3471_cov_3.428571, whole genome shotgun sequence.
ACCESSION   DAOPID010000164 DAOPID010000000
VERSION     DAOPID010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37401_length_3471_cov_3.428571"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            276..2102
                     /locus_tag="PK641_05725"
     CDS             276..2102
                     /locus_tag="PK641_05725"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPG55880.1"
                     /translation="MKKLSFILLLVAMSVSSYALDILSGTFYFDNSKTQYGSVKFLYG
                     SDYQDYTVIVDLIDLGNGKWELTIPSTVHDMYRYSFSNTSYAAGVYQSTFSNMKDEIS
                     NVRMELRTATTDQEMVVGATYTPASGSNWAQGSWISPYSGGCYNLSNTLPVFYLNTVS
                     GDEITSKEVYVPATMYIDAMGQTGYQSYGTQQNPINTEVKGRGNYTWIGFDKKPYRIK
                     METSASLLNLSQSKSFCLLAHADDDCAFMRNTVGFELSRIFGLPYTPGQEPVELVLNG
                     QYWGLYFLTDHITVSTTRVNIIEQADNSIDPLEITGGWLLEIDNYDDVNQIVIQENED
                     EIIRFTYKSPEILSYEQESYMTTFLENANAAIYNPDKNSTDWENYIDLESAANFYIVQ
                     EIMGNAEGFHGSCYLNKDRGNNTKLNFGPVWDFGNSYRHSGFIYDDPPFGDNWISELV
                     KFPRFQSKVQQRWNDESWRLSTIYDLMDQLATRISSAANCDCGKWPSYSSSNFQNGLI
                     QARSYLNNRISWLNTLWQTTGVEDVDDEINVSVYPNPSMGNVYVSSDADIISAELCDL
                     SGRLLQTFDCREFPLVLNVSAGTYLMRVYTNNNMSVHKILVR"
     gene            2181..3377
                     /locus_tag="PK641_05730"
     CDS             2181..3377
                     /locus_tag="PK641_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPG55881.1"
                     /translation="MPVEEKLLNANYVKVMFVNFAVSFAFYLLAPLLPLYLSETFNAS
                     KDIIGLVLSGYTITALICRPFSGYIVDSFPRKMVLEICLLVYFLFFLGYLIAGSLLVF
                     AIFRTLHGGPFGASTVVNSTMAIDVSYPSRRKEAIGYYGLSNNIATALAPTIGIFLYD
                     YCNNFEVLFWVAFIFAGVGLFVGYTIKLFEKQTVNNRSKLSLDRFFLLRAWTIGVNIA
                     FFGFCFGVISNYLAIYGQEMLGITSGTGVYFMLLSGGLILSRFQGAKSLKEGKLTQNA
                     AVGIIVSTVGFSIFVGLPNLVGYYLSALLIGLGNGHMWPAFQNMIIGIAHHNERGTAN
                     STLLTSWDLGVGVGILLGGVIAEYFGYKWAFISVVIVHLIGLIMYFAKTQKSYLDRKS
                     TMQDEL"
BASE COUNT         1073 a          490 c          697 g         1211 t
ORIGIN      
        1 gggtagtgct tgtgatacat ctccaaggcc taaacgtatt acatctgctt tggggtgtac
       61 gactttgtgc gcattaatct tttgctctat ttcgttgaag aaatagcatt caggtagttt
      121 taagtagttt tcgttaatta aggtcataac ttgcaaatgt aaaaaaacat tgataaatat
      181 aaaaatattt tattattttt gtttgttata aaggcgtaaa aaattgtata attaaatatt
      241 aaaatatatc tttgtaatac cttaaaaaat aatatatgaa aaaattgtcg tttattctac
      301 ttcttgtagc tatgtcagtt agcagttatg ctcttgatat attatccggt acattttatt
      361 tcgataactc taagacacaa tatgggtctg tgaaattttt atacggatca gactaccaag
      421 actacactgt tattgttgat ttaattgatt taggtaatgg aaaatgggaa ttgactattc
      481 catctactgt tcatgatatg tatcgttata gcttctcaaa tacatcttat gcagccggtg
      541 tgtatcaaag tacgttctcg aatatgaagg atgaaatttc taatgttcgc atggaattaa
      601 gaacagctac aactgatcaa gagatggttg ttggtgcaac atatacacct gcaagcggta
      661 gtaattgggc acaaggatct tggatatcac catatagtgg tggttgctat aatctttcaa
      721 atactttacc tgtgttttat ctcaacaccg taagtggtga tgaaataact tcaaaagaag
      781 tatacgttcc ggctaccatg tatattgatg ctatgggtca aaccggttat caatcttatg
      841 gtacccaaca gaatccgatt aatactgaag taaaagggcg cggtaactat acttggattg
      901 gttttgataa gaagccttat cgtataaaaa tggaaacgtc ggcgtcttta ctaaacttaa
      961 gtcagagcaa aagtttttgt ttgttggccc atgctgatga tgattgtgca tttatgcgca
     1021 atactgtagg atttgaattg agccgtattt ttggtttgcc atatacaccg ggtcaggaac
     1081 ctgttgaatt agttctgaac ggacaatatt ggggtttgta cttcttaaca gatcatataa
     1141 cagtttcaac taccagagta aatataatcg aacaagctga caatagcata gatccattag
     1201 aaataacagg aggttggttg ctggaaattg ataattacga tgatgtaaac caaatagtaa
     1261 tacaagaaaa tgaagatgag ataattcgat tcacatataa atcgcctgag atattgtctt
     1321 atgaacagga atcatatatg acaacattct tagagaatgc taatgctgct atttacaacc
     1381 cggataagaa tagtactgat tgggaaaatt atattgattt ggaatcggca gctaattttt
     1441 atatagttca ggaaataatg ggtaatgccg aaggctttca tggcagttgt tatttgaata
     1501 aggatagagg gaataatact aagctgaatt ttggaccggt atgggatttt ggaaattcgt
     1561 acaggcattc aggttttata tacgatgatc ctccttttgg tgataattgg atttctgagt
     1621 tggttaaatt tcctcgtttt caatcaaagg ttcaacaacg ttggaatgat gagtcttgga
     1681 ggttatcaac tatatatgat ttaatggatc aattagcgac acgtataagt tcagctgcca
     1741 attgcgattg tgggaaatgg ccttcttatt catcatctaa ttttcaaaat ggtttaattc
     1801 aggcacgtag ttatttgaat aatagaatct catggcttaa tacattatgg caaactactg
     1861 gtgttgagga tgttgatgat gaaattaatg tgagcgtata tccaaaccca agtatgggga
     1921 atgtttatgt gtcgtctgat gcagatatta tttcggcaga gttatgtgat ttgtcgggta
     1981 gattattgca aacttttgat tgccgcgaat ttccattggt tctcaatgtg tcggcaggta
     2041 catatttaat gcgtgtatat acaaataata atatgtcggt acataagata ttagtgcgct
     2101 aataatctta ctatatcttt aaacatttag ctccaaatca aaacaaaatt tgtaattttg
     2161 ctcgtcgaaa aattctatat atgcccgtcg aggaaaagct tttgaatgcg aattacgtaa
     2221 aagtgatgtt tgtcaatttt gctgtatcat ttgcattcta tctattagca ccattactac
     2281 ctttatatct tagcgagacc tttaatgcga gtaaagacat aatagggctc gttttatcgg
     2341 gatatacaat aactgcactt atttgtaggc cattcagcgg atatattgtt gatagttttc
     2401 ctaggaaaat ggtattagaa atatgcttat tagtatattt tttattcttt ctcggttatt
     2461 taatagcagg ctcattactt gtgtttgcta tattcaggac tctgcatggt ggtccttttg
     2521 gtgcatcaac agtagtaaat agtacaatgg cgattgatgt atcatatcca agtcgtagaa
     2581 aagaggccat tggttattat gggcttagta ataatatagc aacagcacta gctcctacaa
     2641 taggtatttt cttgtacgat tattgtaata attttgaggt tctattttgg gttgcattca
     2701 tttttgcagg tgtaggtctg tttgtggggt atacaatcaa actatttgaa aaacaaacag
     2761 taaacaatag aagcaaactt tcgttagata gattcttttt gttaagggct tggacgatag
     2821 gggttaatat tgcatttttc ggcttctgtt ttggtgttat aagtaattat ttggctattt
     2881 atggtcaaga aatgctaggc ataacaagtg ggacaggggt gtattttatg ctactttccg
     2941 gcggtctgat attatcaaga tttcagggtg caaaatcatt aaaagaaggc aagttaactc
     3001 agaatgcagc tgttggtatt attgtttcta cagtaggttt ttcaatattt gtcgggctgc
     3061 ctaatttagt agggtattat ctttcggctt tgctaatagg gttaggtaat ggacacatgt
     3121 ggcccgcatt tcaaaatatg attattggta ttgctcatca caacgaacgc ggtacagcaa
     3181 actcgactct acttacttca tgggatttag gcgtaggtgt gggaatatta ttgggaggag
     3241 taattgcaga atattttggt tataaatggg cctttatatc agttgtcatt gttcatctta
     3301 ttggtcttat aatgtatttt gcaaaaacac aaaagtcata tttagatcgt aaatctacta
     3361 tgcaagatga actatagtca cacattataa cttatgatat taaattatca tgactttcta
     3421 atattgtgac cttgtgagga gtacaaagta tgtttttagc aaaaaactaa a
//
LOCUS       DAOPID010000165         3432 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_38020_length_3432_cov_4.262659, whole genome shotgun sequence.
ACCESSION   DAOPID010000165 DAOPID010000000
VERSION     DAOPID010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38020_length_3432_cov_4.262659"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..1356)
                     /locus_tag="PK641_05735"
     CDS             complement(13..1356)
                     /locus_tag="PK641_05735"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPG55882.1"
                     /translation="MRKFVLLLFMAVFVSVELFGAEFSISGTDAVGTESTIDTIFVVN
                     GTENGVLHFQMPESHDYNWYKYDIDGTSSLIKSETNVTETSIDLEDEKSIGYKLDISG
                     NKSAWVWVFDYKRYQLLLDTIIVNGEVEDRCSYIQLNINMDVEPMEYDTIGQITMHDE
                     VERLFELSYDSTYYSKGSGYVSEEVTRVIPELSDYTIESPLGDTQYSLTGDQFAKVFE
                     EEPPVIVSEPFKAIAVEVHLDATVRIRENAKNENEKGDPIEPDETKTLLKGSAPLNIE
                     IINNASPASFFYTWCLSNEREFEDCIIQTTDKDFRYTFTEKATYYLRVQVSNSSVTED
                     PEIGCTKEATYTIEVLESALDIPNIFTPNGDGKNDEFKVAYKSIIKFNASVYNIWGKL
                     VYQWDDPTTGWNGNIGGKPAAEGAYMYIIEATGDDLDDKGNRIKYYKKGTVNLIR"
     gene            1504..2949
                     /locus_tag="PK641_05740"
     CDS             1504..2949
                     /locus_tag="PK641_05740"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPG55883.1"
                     /translation="MKRSIFCIITMLAITSIILAQNNQYQIITENGKQYYLYTVQKSE
                     GFYSIGKRFEITNDEIIKANPEAALGLKLGQVLKIPVYKSESESESISKPSTSVEPTE
                     TNLENQDNENKNTIVDEPSQTVLKYNRTKKSSIRIAILMPFSLDAVERDANMDHFVEF
                     YQGCLIAVDSLKSLGIDVFIDSYDIGKTTNKLDLILHQAALKQADLLIGPAYSSQVPH
                     IADFAKINKIKVVVPFTTNIPDLENNQYLFQIVSPQKELYNKIADKCANQWENKQVLI
                     VRPDSAGIRYDKKDFVKILIPKLRAKNISVKYISETRIAANIDSAAKSNMRETVVVIP
                     TSNKVKLIQLSEHFSRIESNNVSLFGFPEWHNYQIKDLYVKPLYTFSNYNTDFSSTKV
                     ISFYQKYNEKFGIPGVQNSPSYTIFGYDICNYFISQWIENGEHFENFLNDEETPLLQM
                     KFLFERVGNGGYLNRGTIFQLYNDEGILDIE"
     gene            2951..>3432
                     /locus_tag="PK641_05745"
     CDS             2951..>3432
                     /locus_tag="PK641_05745"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPG55884.1"
                     /translation="MKRRLITIFVGMFVLCVSASAQYTYIQPELAIGTNQGVAIWTTV
                     GFSPSVAQNFDVRYNGGFTVRYIIQKHFGIQAELNYAQRGWSETSSTGERYDHRFDYL
                     EIPLVAHIYFGKEKFRFFVNLGPKFRYMINDYATEHFSSNYGKQQTKAIENRFDYSVF
                     G"
BASE COUNT         1205 a          605 c          513 g         1109 t
ORIGIN      
        1 atatttttac aattatctaa taagattaac agtacccttc ttgtagtact ttattctgtt
       61 tcctttatca tccagatcat cacctgttgc ctctataata tacatgtatg caccttcagc
      121 agcaggttta ccacctatat ttccattcca acctgttgta ggatcatccc attgataaac
      181 taatttaccc cagatattat agacagaagc attaaacttg ataattgatt tataagctac
      241 tttaaattca tcgtttttgc catctccatt aggagtaaat atatttggaa tatctaaggc
      301 agattctaaa acttcaattg tatatgtagc ctctttggta caacctatct caggatcttc
      361 ggtaacagac gaatttgata cttgtactct taaatagtaa gtagctttct cggtaaatgt
      421 atatctaaaa tccttgtcag tagtttgaat gatgcaatcc tcgaattcac gttcatttga
      481 caaacaccag gtataaaaaa atgatgcggg agaagcatta ttaataattt ctatatttaa
      541 gggtgcagaa cctttaagta atgtcttagt ttcatcgggt tcaattggat cacctttctc
      601 gttttcgttt ttagcatttt ctcttatacg tacagttgca tctaagtgaa cttcaactgc
      661 tatggcttta aacggttcag atacaattac cggcggttct tcttcaaaaa ctttagcaaa
      721 ttgatcaccc gtcaaagaat attgcgtgtc tcctaatggc gattctatgg tataatcaga
      781 aagttcagga ataacacgag ttacttcttc agaaacataa ccgctgcctt tgctatagta
      841 tgtcgaatca taacttaatt caaatagtct ttctacttca tcgtgcatag ttatctgccc
      901 aatagtatca tattccattg gctctacatc catatttata tttaattgaa tgtatgaaca
      961 tctatcctca acttctccat ttacaattat tgtatcaagc aataattgat atcttttata
     1021 gtcaaatacc caaacccaag cacttttatt tccggaaata tccaacttgt aacctattga
     1081 tttctcatcc tccaaatcaa tgctggtttc ggtaacattt gtctcggatt taatcaaaga
     1141 acttgtgccg tcaatatcat atttatacca attatagtcg tgcgattcag gcatttgaaa
     1201 atgcaacaca ccattttctg taccattaac aacaaatata gtatcaattg tagattctgt
     1261 accgactgca tctgtaccgc ttatcgaaaa ttctgctcca aaaagttcaa cagacacgaa
     1321 aacagccata aataataaca aaacaaattt tcgcatatta tatttgtgct tataaaatca
     1381 aaaaaaaatt aaatattact acactacccc acaaatcacc gtgtaaaggt aaggcttttg
     1441 gctttatctg ccaaaaattt gtatctttgc aacttgtagt agcatatttt aataggaatt
     1501 gacatgaaac gttctatctt ttgcattatc acaatgcttg caataacaag cattatatta
     1561 gctcaaaata atcaatacca aataattaca gaaaacggta aacaatacta cttatatacc
     1621 gttcagaaaa gcgaaggatt ttacagcata ggaaaacgtt tcgaaattac aaacgacgaa
     1681 attattaagg ctaatcctga agcagcgctt ggacttaaac ttggacaagt acttaaaatc
     1741 cctgtatata aatctgaatc tgaatctgaa tctatatcta aaccaagcac atctgtagaa
     1801 ccaactgaaa caaatttaga aaatcaagat aacgaaaaca aaaacacaat tgttgacgag
     1861 ccatctcaaa ctgttttaaa atataatcgt accaaaaaat caagtataag aattgctata
     1921 ctaatgccat tttcgttgga tgcagttgag agagacgcaa acatggacca ttttgtcgaa
     1981 ttttatcaag gatgtttaat agcagttgat tcattaaaga gtttaggaat agatgttttt
     2041 attgacagct atgatatagg taaaacaact aacaaattag atttaatatt acatcaagca
     2101 gccttaaaac aggcagattt actcatcgga ccggcttatt caagtcaagt tcctcatatt
     2161 gccgattttg caaaaataaa caaaataaaa gtagttgtac catttacaac caatatccct
     2221 gatttagaaa acaatcagta tttatttcaa attgtaagcc cacaaaaaga attatataat
     2281 aaaatagctg ataaatgtgc taatcagtgg gagaacaaac aagtacttat tgtacgtccg
     2341 gatagtgccg ggattagata cgacaaaaaa gattttgtaa aaattcttat cccaaaatta
     2401 cgcgcaaaaa acatttctgt taaatatatt tctgaaacac gtatagcagc taatatagat
     2461 tctgctgcta aaagcaatat gcgcgaaact gtggtggtaa ttccaacttc aaacaaagta
     2521 aaactaattc aattatcaga gcatttttct cgaatagaga gtaataatgt tagtttattt
     2581 ggattcccgg aatggcataa ctatcaaatc aaagacttgt acgtcaaacc attatatact
     2641 tttagcaatt acaatactga tttttcatca acaaaagtaa tttcattcta tcaaaagtac
     2701 aatgaaaaat tcggtatccc cggagtacaa aactcaccta gttataccat tttcggatat
     2761 gacatttgta attattttat ttcacaatgg atcgaaaacg gagaacactt tgaaaacttt
     2821 ttaaatgacg aagaaacgcc tttacttcaa atgaaattct tatttgaacg agttggtaat
     2881 ggtggatatt taaatagagg tactatcttt caactatata atgatgaagg tattcttgac
     2941 atagaataag atgaaaagac gactaatcac tatatttgta ggtatgtttg tattatgcgt
     3001 gagcgcatca gctcaataca catacataca gcctgaattg gcaataggca cgaaccaagg
     3061 tgtcgctatt tggacaacag taggcttttc accatctgta gctcaaaact ttgatgttcg
     3121 ctataatggt ggttttactg taagatatat tatacaaaag cactttggaa tacaagccga
     3181 attgaattat gcccaacgtg gatggtctga gacttcttcg acaggcgaaa gatatgatca
     3241 taggtttgat tatttagaaa ttccgctggt tgcccacata tattttggaa aagaaaaatt
     3301 tcgttttttt gttaatttag gtcctaaatt cagatacatg attaatgact atgctacaga
     3361 gcatttttca tctaattatg ggaaacaaca aaccaaagct attgaaaata gatttgatta
     3421 tagtgtattc gg
//
LOCUS       DAOPID010000166         3399 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_38566_length_3399_cov_4.212620, whole genome shotgun sequence.
ACCESSION   DAOPID010000166 DAOPID010000000
VERSION     DAOPID010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38566_length_3399_cov_4.212620"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..657)
                     /locus_tag="PK641_05750"
     CDS             complement(118..657)
                     /locus_tag="PK641_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55885.1"
                     /translation="MKTKIALWMLSKLESKQKRCFMNLSQIRSLHILYLIDKPLNIDA
                     QKQYNDLYAILDFCMAQKIKTEITLFVDKSLEEQKRFNIHILTAKDFSKWTWKPLDAT
                     YADFMELDCEVLLNLSPKTCYPLEYLAAMSNAKFKIANKREIQGAKYDFMIEMQGEQD
                     TPKSMFDTVMFYLEKIQSK"
     gene            complement(672..2675)
                     /gene="ligA"
                     /locus_tag="PK641_05755"
     CDS             complement(672..2675)
                     /gene="ligA"
                     /locus_tag="PK641_05755"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016267567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPG55886.1"
                     /translation="MDATLQKIENLRDLLNRYNHEYYVLSQPTISDFEFDQLMSELKN
                     LETVYPQYADPNSPTMRVGSDINQSFKQIKHKYLMLSLGNTYSEAEITDFYERIKKTL
                     NEPFEIVCELKYDGTSISLTYEHGKLIQAVTRGDGEKGDDVTANVRTIRSIPLTLNGN
                     YPDLFEVRGEILMPWSVFENLNKEREEQEEPLFANPRNAASGTLKLQNSAIVAKRKLD
                     SYMYYMLGEQLPANNHYDNLKEAELYGFKISGFTKKCSTLQEIYDFINYWDFERKNLP
                     VATDGIVLKVNSLLQQKQLGFTAKSPRWAIAYKFKAERACTRLNSISYQVGRTGVITP
                     VANLDPVQLAGTIVKRASLHNADIIKALDLYVGDMVYVEKGGEIIPKITGVNTDARCL
                     IGDKIGFIDKCPECGAKLVREDGEAGHYCPNEDNCPPQIKGKIEHFVSRKAMNIDGLG
                     EEIVDLLYRNKLIETSADLYKLKLVDLAYLDRLGEKSGRKIIQSIIDSKQVPFERVLF
                     ALGIRYVGATVAKKLAYSLKNIDNIAAASLETLTSIEEIGERIAQSVYDYFRIDKHIK
                     LIENLRSAGLQFQIAEDVFEGYTDTLQGKIFVISGVFAKYSRDELKNLIERYGGKNAG
                     SISAKTNYVLAGNNMGPAKLEKANSLNVPIIDEDTFLNMIGEL"
     gene            2810..>3399
                     /gene="trmD"
                     /locus_tag="PK641_05760"
     CDS             2810..>3399
                     /gene="trmD"
                     /locus_tag="PK641_05760"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005837186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPG55887.1"
                     /translation="MRIDIISVLPELIESNFSYSILKRAQNKGLAEIYLHNLRDYSTD
                     IKHHRVDDYPFGGEAGMVMQIEPVDRIISKLKSERDYDEVIFTSPDGEVFNQHIANDM
                     SLMNNIIILCGHYKGIDYRIREHLITREISVGDYVLTGGELAASIIVDAVVRIIPGVI
                     SDEQSALSDCFQDDLLSAPVYTRPADYKGWKVPDVL"
BASE COUNT         1067 a          640 c          509 g         1183 t
ORIGIN      
        1 atcaacagaa tcatctgttt gaaatggtgt tatcaaagca acacccatac cttttaaatt
       61 agttgccatc gtatattccg tagcttgtta gcggggcaaa gttagctatt tttttagtta
      121 ctttgattga attttttcta aataaaacat tactgtatca aacatcgatt ttggggtatc
      181 ttgttcgcct tgcatttcta tcataaaatc gtattttgca ccttgaatct ccctcttgtt
      241 tgctatttta aactttgcat tcgacattgc tgccaaatat tccaaaggat aacaggtctt
      301 tggacttaag tttaataaaa cctcgcaatc taactccata aaatcagcat aggtagcatc
      361 taatggcttc caagtccatt tgctaaagtc ttttgctgta agtatatgaa tattaaatcg
      421 tttttgctct tccaatgatt tatcaacaaa cagagtgatt tctgttttta ttttttgtgc
      481 catacaaaag tctaatattg catacaaatc attatactgt ttctgtgcat caatatttag
      541 aggcttgtct attaaataca gaatgtgcaa acttcttatc tgactaagat tcataaagca
      601 tcgtttttgt ttcgattcta atttcgacag catccacaat gcgattttag ttttcatttg
      661 tgttattgca ttcataattc ccctatcatg tttaaaaatg tatcttcatc aataataggc
      721 acatttaaac tatttgcctt ctctaatttt gccggtccca tattattgcc ggcaagaaca
      781 taattggttt tggcagaaat actaccggca ttttttcctc cataacgttc tattaagttt
      841 ttaagttcgt cacgcgagta tttggcaaaa actccactaa taacgaaaat tttaccttgc
      901 aatgtatcag tgtaaccttc aaaaacatct tcagcaattt gaaattgtaa gcctgctgaa
      961 cgtaaatttt caatcagttt tatatgttta tctattctaa aataatcata tacactttga
     1021 gcaatacgtt cacctatttc ttcaatgctt gtaagtgttt cgagcgatgc agctgcaata
     1081 ttgtcaatat tcttcaatga ataagctaac tttttggcaa cggtagcacc aacatatctt
     1141 ataccaagag caaacaggac acgttcaaat ggtacttgct tactatcaat aatactttga
     1201 atgatttttc ggcccgattt ttcacccaat ctatcaagat atgccaaatc aactaacttc
     1261 aatttatata agtctgctga tgtctctatt aatttgttgc gatataataa atcaactatt
     1321 tcttctccaa gtccatcaat attcattgcc tttctcgaga cgaaatgctc tattttacct
     1381 ttaatctggg gcgggcaatt atcttcatta gggcaatagt ggcctgcttc tccgtcttca
     1441 cgtacaagct tagcaccaca ttcagggcat ttatctataa atccgatttt atctccaatt
     1501 aaacatcttg catcagtatt aacaccggtt atttttggaa tgatttcccc tcctttttca
     1561 acatatacca tatcaccaac atacaaatcc aaggctttta tgatatctgc attatgtaat
     1621 gatgcacgtt taacaatagt ccctgccaat tgaaccggat ctaagtttgc tacaggagtt
     1681 attactccgg tacgtcccac ttgatatgat attgaattta agcgtgtaca ggctctttct
     1741 gccttaaact tatatgcaat agcccatcgc ggagattttg ccgtaaaacc cagttgtttc
     1801 tgctgtagca atgaattaac cttcagaact ataccatcag ttgcaaccgg caaatttttc
     1861 ctttcaaaat cccaataatt aataaaatca taaatttctt gtaaggtaga acacttcttt
     1921 gtaaaaccgg atattttaaa accatataat tcagcctctt ttaagttatc ataatgatta
     1981 ttagccggta attgctcacc aagcatataa tacatatatg aatctaactt ccttttagct
     2041 acaatggcag aattttgaag ttttaatgtt ccggacgctg catttcgagg attagcaaat
     2101 aaaggttcct cttgttcctc acgttcctta ttcaaatttt caaaaacgct ccatggcatc
     2161 aaaatttctc cacgcacctc aaataaatca ggataattgc catttaatgt tagtgggatg
     2221 cttctaatag ttctaacatt tgccgtaaca tcatcaccct tctcaccatc accacgagtt
     2281 actgcctgaa ttaatttacc atgttcgtat gttagagaaa tagaagttcc atcatatttc
     2341 aattcacaca caatctcaaa aggctcgtta agtgtttttt taatacgttc ataaaaatct
     2401 gtaatctctg cttccgaata ggtgtttcct aacgacagca tcaagtattt atgttttatt
     2461 tgcttgaatg actgattaat atcactacca acacgcattg ttggcgaatt cggatcagca
     2521 tattgcggat aaactgtttc caaattcttt aactctgaca ttaattgatc aaactcgaaa
     2581 tctgaaatcg ttggttgcga taatacataa tactcgtgat tataacgatt aagcaaatcg
     2641 cgcagatttt caattttttg taaagttgca tccattaaga ttacagagta aatttcttca
     2701 attcacaaag ataaaaaaag aaaagagatt ctaatagatt caattctaag aaaacaataa
     2761 aaaaacgtac ctttgcatta gttattgaat aaaatttaat ataacatata tgcgtattga
     2821 cataatatca gtacttcctg aacttattga aagtaatttt tcgtattcaa tactaaaacg
     2881 agctcaaaat aagggtttag ctgaaatata tctgcataat ctaagagatt attcgacaga
     2941 tattaaacac catagggttg acgattatcc ttttggagga gaagccggta tggttatgca
     3001 aatagagcct gttgacagaa taatcagcaa acttaaaagt gaacgtgatt atgatgaagt
     3061 gatttttaca tcacctgatg gtgaagtttt taatcagcat atagcaaatg acatgtctct
     3121 tatgaacaac ataattattt tatgtggtca ttataaaggc atagattaca gaattcgtga
     3181 acatcttatt acacgtgaaa tatcagttgg agattatgtt ttaacaggtg gcgaattggc
     3241 agcatctata attgttgatg cagttgttag aataataccc ggagtgattt ctgatgagca
     3301 atcagcccta tccgattgtt tccaagatga cttactttcg gctcctgttt acacacgtcc
     3361 ggcagattat aagggctgga aagtacctga tgtcttatt
//
LOCUS       DAOPID010000167         3390 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_38701_length_3390_cov_5.938231, whole genome shotgun sequence.
ACCESSION   DAOPID010000167 DAOPID010000000
VERSION     DAOPID010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38701_length_3390_cov_5.938231"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2151)
                     /locus_tag="PK641_05765"
     CDS             complement(<1..2151)
                     /locus_tag="PK641_05765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPG55888.1"
                     /translation="MITSNLKKSRKAILTAALLVLGLCAYAQQLAVKGSVLDEAGEPI
                     IGAYITLEGNNSVGTITDFDGNFEISVDAKSKLLVSYTGYESQTIAVAGKSQIDVVLR
                     EISTHLDEVVAIGYGSQKAKEITSSVASVKAENFNPGVKESPMGLLQGKVAGLTITRT
                     EGGDPTKTGFNVQIRGTSTLDKGSGTTPLYIVDGIPVTSIDNIAPEEIASMDVLKDGS
                     SAAIYGTRGTNGVIMITTKRGTGSGNVAECGTTNVEYSGYASFAIDASKQQMATADQF
                     LEMQSMTNGKCKASDKGYKTDWVSAQMRDFALTHNHNLAISGATKNFSYRGSVNFKNA
                     EGIANTSNRQEIMAKLAANQKALQGWLDLQYDFSYMHYKNDYFCGSFDNGAIMNPTMP
                     IYNEDGSYYIPTGTVTSNPIADNNLKESYQDGNYFRGSVKASVNIKPVPGLKVSGFGA
                     FEEGDNYDYWSNSKKYYKASDANLAGRKTKRSMNQLYEGTIDYVNEWNGHALAAVLGT
                     SYQNFWYDGSEIENGGFAVDGIKYYKIGDGDVEKTNMKMSSYRNSNTLVSFFARANYN
                     YKEKYLLSASVRREGSSRMGANFKWGWFPAASAGWRISGEDFLKDVKAVNDLKLRFGF
                     GITGNNLKDDLKSVELLTQGGTFNNHGTEAYSYTVNQNANPNLAWERKFEYNLGIDYA
                     FLDNRLYGSIDLYFRNTKDLLWEYNVPTPPYQYST"
     gene            2738..>3390
                     /locus_tag="PK641_05770"
     CDS             2738..>3390
                     /locus_tag="PK641_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPG55889.1"
                     /translation="MKSQITIKDIARELNISASTVSRALQDHPDISKATKEAVNAYAK
                     KYHYKPNAIALSLKMKRTNVIGVLVPEMVHHFFSSVFAGIEDVANEKGYNVIVCQTSE
                     KYEKEVKNVETLISARVSGVLASISKETEKYDHFQELIDDGTPLVFFDRICTGLKTDR
                     VIADDYTGAYNAVEHLIKTGCKRIAFYGAPANLEISKNRKNGYIDALRANSIGIDNSL
                     "
BASE COUNT         1125 a          659 c          529 g         1077 t
ORIGIN      
        1 tgtagagtat tgataaggag gagttggcac gttgtattcc cacaacaaat cttttgtatt
       61 acggaagtat aaatcgatag atccatataa acgattatct aagaatgcat agtcaatacc
      121 aagattatac tcaaacttac gttcccatgc caagttagga tttgcatttt gatttactgt
      181 atatgaatat gcttcagttc catgattatt gaaagtacca ccttgagtta acaactcaac
      241 agacttcaga tcatctttca aattattacc agtaatacca aatccgaaac gtagtttcaa
      301 gtcattaact gccttaacat ctttcaaaaa gtcctcaccg ctgatacgcc aacctgcgct
      361 cgctgctggg aaccaacccc atttgaaatt ggcacccata cgcgaagaac cttcacgacg
      421 tacagaagca gataacaaat atttttcctt ataattgtaa ttagcgcgag caaagaaaga
      481 aaccaaagta tttgagttgc gataagaact cattttcatg ttagtttttt ctacatcacc
      541 atcgccaatc ttataatatt ttattccatc aaccgcaaaa ccgccgtttt cgatctcaga
      601 accatcatac cagaaatttt gataagacgt acctaaaaca gcagccaaag catgaccatt
      661 ccattcatta acataatcaa ttgttccttc atataattga ttcatgcttc tttttgtttt
      721 acgtcctgcc aaattggcat cagatgcttt ataatatttt ttactgtttg accaataatc
      781 ataattgtca ccttcttcaa atgcaccaaa tccgctaact ttcaaaccag gaaccggctt
      841 aatgtttaca gaggccttga cagatcctct aaagtaattt ccatcttgat aagattcttt
      901 aagattgtta tcagctattg gattcgaagt gacggttccg gttggaatat aatatgaacc
      961 atcttcattg tatattggca ttgtcggatt catgatagcg ccattatcaa aactaccaca
     1021 gaaataatca ttcttatagt gcatataaga aaagtcatat tgcaaatcca accaaccttg
     1081 taatgctttt tgatttgcag ccaatttagc cattatttct tgacgatttg aagtattcgc
     1141 tataccttca gcatttttga agtttacaga accacgatag ctaaaatttt tggtagctcc
     1201 cgatatagca agattatgat tgtgcgttaa tgcaaaatcg cgcatttggg cactaaccca
     1261 atctgtttta tatcctttat cagaggcttt acatttacca ttagtcatgg attgcatttc
     1321 taaaaattgg tcagcggttg ccatttgctg tttgctggca tctattgcaa aagaagcata
     1381 acctgaatat tctacattag ttgtaccgca ttcagccaca ttgcctgaac ctgtacctct
     1441 tttggttgtt atcatgatta caccgttagt accacgtgta ccgtaaattg cagcagaaga
     1501 tccgtctttt aaaacatcca ttgatgcgat ttcttccgga gctatattat caatacttgt
     1561 aacaggaata ccatcaacta tatatagtgg agttgtaccc gaacctttat ccaaagttga
     1621 tgtaccacga atttgaacat taaatcctgt ttttgtagga tcgccaccct cggtacgagt
     1681 tattgtaaga ccagctactt taccttgtaa aagtcccatc gggctttctt taacaccagg
     1741 attaaagttt tctgctttta cgcttgctac agaagatgtg atttctttgg ctttttgcga
     1801 accataacca atagccacaa cctcatccaa atgagttgat atctcacgta aaacaacgtc
     1861 tatttggctt ttacctgcca ctgctatagt ctgagattcg taaccggtgt aagatacaag
     1921 aagtttcgac tttgcatcta cagatatttc aaagttgcca tcaaagtctg tgatggttcc
     1981 gacgctatta ttaccttcca aagtaatata cgcaccgata atcggttctc cagcctcatc
     2041 taacacgcta cccttaactg caagttgttg cgcataggca catagcccta gcaccaacaa
     2101 agccgcggta agaatagctt ttcgtgattt ttttaagtta gaagtaatca tgcattgaat
     2161 tttttaagag ttaataaata agttttgtta aatttctgtt tctcaaaaac acaacatgct
     2221 ttgcagcata tttgcattcg tttgcgcgcc aaatggcagt tttacaatcg acaaacgtac
     2281 actatttttt atattagaac aaattttatg gagttttttt tgcaaaatga tagatttact
     2341 agtcgaaatg ttaaaaaaac aactgatgta gtgttgatgt attttcaaaa atcatttaaa
     2401 tatccattgc agcacattat aaccggtttt taaaatacaa acgagtgcga caagttctgc
     2461 gaacataata gaaatttagt aataaaagag cagtttaata ttaacaaatt ctatatatca
     2521 tctttttaac aaataaatta catcatcaac tcaacaaata ttaactcaca taaatgtttt
     2581 tttcttttga actaccacat ttgtatacaa cgctttgctt tttcacaaaa atatatatag
     2641 ctttgtacct taattaattt aatataatta tagcaacaaa acaaaaacgc tattttatat
     2701 taaatctatt acaaaattac aataataaaa attactcatg aaaagccaaa tcacaatcaa
     2761 agacattgca agggaattga atatttctgc atcaacagtg tctcgtgctt tacaagatca
     2821 tccggacatt agcaaggcaa caaaagaagc agttaatgcc tatgctaaaa aatatcatta
     2881 caaaccgaat gcaattgctt taagtttgaa aatgaaacgc acaaatgtaa tcggtgttct
     2941 tgtgccggaa atggttcatc acttcttttc atcagtattt gccgggattg aagatgtagc
     3001 taacgaaaaa ggctacaacg taattgtttg tcaaacttca gaaaaatacg aaaaagaagt
     3061 aaaaaatgtt gaaactctaa tatcagctcg tgtatcgggt gtattagctt caatatcaaa
     3121 agagacagaa aaatacgacc actttcaaga actaattgac gatggtacac ctctagtgtt
     3181 ctttgataga atatgcacag gcttgaaaac agaccgtgta atagcagacg actataccgg
     3241 tgcatataat gctgtagaac atcttatcaa aacaggttgc aaacgcattg cattttatgg
     3301 agctccggca aacttggaaa tctcaaaaaa tagaaagaat ggatatatcg atgctttaag
     3361 agctaatagc attggcattg acaatagcct
//
LOCUS       DAOPID010000168         3388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_38723_length_3388_cov_5.566757, whole genome shotgun sequence.
ACCESSION   DAOPID010000168 DAOPID010000000
VERSION     DAOPID010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38723_length_3388_cov_5.566757"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PK641_05775"
     CDS             complement(<1..333)
                     /locus_tag="PK641_05775"
                     /inference="COORDINATES: protein motif:HMM:NF024982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4138 domain-containing protein"
                     /protein_id="HPG55890.1"
                     /translation="MKIIRHLIIVLFGMSCLTANALEDSLAVSNLHTVILDFKSEIKY
                     VDFGSSEIFGSKLSSGKMLSIQAKVPDFHETTLSVVTGDGKFYEFKVYYEKNPNFAVW
                     NNESLISSK"
     gene            complement(320..1441)
                     /gene="traM"
                     /locus_tag="PK641_05780"
     CDS             complement(320..1441)
                     /gene="traM"
                     /locus_tag="PK641_05780"
                     /inference="COORDINATES: protein motif:HMM:NF023922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transposon protein TraM"
                     /protein_id="HPG55891.1"
                     /translation="MNKQQKFLHFISLPFVLCLCVCFYILRENIEAKAEIDESYKTGL
                     NIEIPKAQETELSESRLDAIKKEELRISREKRRENMECSSFEWLDINPSDDKSDRIEH
                     PSRQANVRQYQLISEKTQSEIIRKELPPAIPDFEKEKEAIMREKRAKLEKELGINFSD
                     YGYKKYEKVDDNNEQKNAYTESTNYIGEEKNLNSSGLANENINDSGFYGLEDADEFHE
                     TNIRAVIHGEHKDVNKGSIIKIRLLENVLLGKTKIPQNTFIYGRLGFSDGRATIKIEN
                     INYKDYIIPFAASIYDRDGFEGIHIPDNTISDTKKDAAGKTISGLDLRLTSPNGLLNT
                     AANAVTGAIKSATQSAVREPKITISSNYSITIKRQNENH"
     gene            complement(1448..1726)
                     /locus_tag="PK641_05785"
     CDS             complement(1448..1726)
                     /locus_tag="PK641_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55892.1"
                     /translation="MKKRKLYLIVFLVLVTFLLCLRMIFSFLYIKREESNRIKVEEEY
                     RVRLDSISLEIDKREMVYKKAYIRNYVDSAKIDRVYELADSLNYEQAD"
     gene            complement(1831..2451)
                     /gene="traK"
                     /locus_tag="PK641_05790"
     CDS             complement(1831..2451)
                     /gene="traK"
                     /locus_tag="PK641_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014298158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transposon protein TraK"
                     /protein_id="HPG55893.1"
                     /translation="MFKSLKNIQGAFALIRIYFILITVISISLGAYAIYESYKFAEMQ
                     REKIYVLDGGKSLLMALSHDVQQNRLAEARSHVTRFHEHFFTISPDKEAIEHHMEYAL
                     ALAGKEAIEKYQRMKEEGFYDRIIAAGINCEIRVDSVLIDESNYPFKVRLFGKTSVIR
                     SSNITYRNLETECELINCSRSDNNPHGFMIEKWRIIDNSDINVINR"
     gene            complement(2444..>3388)
                     /locus_tag="PK641_05795"
     CDS             complement(2444..>3388)
                     /locus_tag="PK641_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55894.1"
                     /translation="ANYADALSATAQLVCGLAAFLYIGTKLWHTWAKGEAIDFYSMLR
                     PFSIGLIIVFFSVFTGFLDTLVAPIGVATEYVRDSTQESIYAAHRSYSELQVKMKDNR
                     AKYEQSKSETKQEKLSVWRTMNRNISEIREGISGVFESSETILLRFSVDLLLMIINIL
                     TMAMSYFYKVFVVTAKIVLVLIGPFSLALSVFPGFESNLKLWIAHYINVSLYIPICNI
                     IGFVQGMLISECLYEPGIKAMQSIMSHTMNDSLLTQMDNCMLMTSVLSIIIGIISIMM
                     YINVPRFAGWILNNDGSSGMISSHNYTSNIVLKGGSDV"
BASE COUNT         1072 a          633 c          474 g         1209 t
ORIGIN      
        1 ttttgaacta ataagacttt cattattcca aaccgcgaaa ttaggatttt tctcgtagta
       61 aactttaaat tcatagaatt tcccatcacc agtaactaca gatagtgtag tttcatgaaa
      121 atcaggtact ttagcctgaa tgctaagcat tttacccgat gaaagtttgg aaccaaatat
      181 ttcgctactt ccgaaatcta catatttaat ttctgatttg aaatctagta taacagtatg
      241 caaattactc acagctaaag agtcttctaa agcatttgcg gttagacacg acatcccaaa
      301 cagaactata atcaaatgtc taatgatttt cattttgtct tttaattgta atagaataat
      361 tagatgaaat agtgatttta ggttctctaa ctgcagattg agtagctgat tttattgctc
      421 ctgttactgc atttgctgct gtgtttaata aaccgtttgg tgaagttagt cttagatcta
      481 aacctgaaat ggtttttcct gcggcatctt ttttagtatc actaatggtg ttatccggga
      541 tatgaattcc ctcaaagcca tctctatcgt aaattgaagc agcaaatggg attatgtaat
      601 ccttatagtt aatgttctct atcttaattg ttgctcttcc atcagaaaat cctaacctac
      661 cataaataaa tgtattttga ggaatcttcg tttttcccaa taaaacgttt tccaacaatc
      721 tgatttttat aatagaaccc ttgtttacat ctttatgctc gccatgtata actgctctta
      781 tattcgtttc atgaaattca tcggcatctt ctaatccata aaatcctgag tcattaatat
      841 tttcatttgc aagccctgag gaatttaaat tcttttcttc tccaatataa tttgtggact
      901 ctgtatacgc attcttctgt tcattattgt catcaacttt ttcatatttt ttatatccgt
      961 aatcagaaaa gtttattcct aactcttttt ctaatttagc acgcttttca cgcataatag
     1021 cttccttctc tttttcgaag tcagggatgg cgggcggcag ttcttttctt atgatttccg
     1081 attgcgtttt ttcggatata agttggtact gccgcacatt cgcctgtcgg gatgggtgtt
     1141 caatccggtc ggatttgtcg tcagatgggt tgatgtccaa ccattcaaag gatgagcatt
     1201 ccatgttttc ccgacgtttt tccctcgata tacgaagttc ttctttttta attgcgtcta
     1261 aacggctttc tgaaagttcg gtttcttgag ccttgggtat ttctatgtta agtcctgttt
     1321 tatatgactc atctatctct gcttttgctt ctatgttttc tcttagtatg taaaaacaaa
     1381 cacataaaca caaaacaaat ggcagtgaaa taaaatgcaa aaatttttgt tgcttgttca
     1441 taatatatca atctgcttgt tcgtaattca aagaatcagc tagttcataa acacggtcta
     1501 ttttagcact atcaacatag tttctaatat acgctttctt gtataccatc tcccgtttat
     1561 ctatttctaa actaatacta tcaagacgaa cacgatattc ttcttcaact ttgattctat
     1621 tactttcctc tcttttaata tataaaaatg aaaaaatcat tcttagacac aacaaaaaag
     1681 ttacaagaac taagaataca ataagatata gttttctttt tttcacagaa agtttattta
     1741 aaacattcga caattcgcta ctatacttgc acaaaaaaat cttgcaccgt gaatatattt
     1801 gacgctttaa actattgttt tttatccttt tcatcgatta attacgttaa tatcactatt
     1861 atctataatt ctccattttt caatcatgaa tccgtgagga ttattatcag atctggaaca
     1921 atttattaat tcacattcag tttctaggtt cctatatgta atatttgaag aacgaataac
     1981 agatgttttc ccaaatagtc taactttaaa cggataatta gattcatcta tcaatacact
     2041 atctactctt atttcgcaat ttattccggc tgcaattatt cgatcgtaaa aaccttcttc
     2101 cttcatacgt tgatattttt caatagcttc ttttcccgcc aaagctaggg catattccat
     2161 gtgatgctct atggcttctt tatctggtga aatcgtaaaa aaatgctcat gaaaacgtgt
     2221 cacatgcgaa cgggcttcag caagtctatt ttgttgtaca tcgtgcgata aagccattaa
     2281 taaagatttc cctccatcca aaacataaat tttctctctc tgcatttcgg caaacttgta
     2341 tgattcataa atagcataag ctcctaacga aatagaaatc acggttatta aaataaaata
     2401 aatacgaatt agtgcaaaag ccccttggat atttttcaga gatttaaaca tctgaacctc
     2461 ctttcaatac gatatttgag gtgtaattat gtgaactaat cataccactg ctgccatcat
     2521 tatttaatat ccagccagca aatctgggca catttatata catcataatt gatataatac
     2581 caatgataat acttaataca cttgtcatca gcatacaatt atccatttgt gttagtaatg
     2641 aatcattcat tgtatgtgac ataatagatt gcattgcttt tataccaggt tcatataaac
     2701 attccgaaat aagcattcct tgaacaaaac ctataatatt acaaatgggt atatataatg
     2761 aaacattaat gtaatgagcg atccataatt ttagattcga ttcaaaacct gggaatacag
     2821 ataaagccaa tgaaaatggt ccaattagta ccaaaactat tttggcagtt actacaaaaa
     2881 ccttataaaa gtatgacatc gccatagtaa gaatatttat aatcatcaat aataaatcga
     2941 ctgaaaaacg aagtaatatg gtttcacttg attcaaaaac accagagatt ccttctctta
     3001 tttctgaaat attcctattc attgtgcgcc atacacttaa tttttcttgt ttcgtttcag
     3061 atttgctttg ttcgtattta gcgcgattgt ccttcatttt tacttgcaat tccgaatagg
     3121 acctatgcgc agcgtaaata gattcttgag ttgaatcacg aacatattca gttgctaccc
     3181 ctattggagc aaccaatgtg tccaaaaaac ctgtaaatac cgagaaaaaa acaattatta
     3241 aaccaattga aaaaggacgt aacattgaat aaaaatcaat agcttcacct tttgcccaag
     3301 tatgccacaa ttttgtacct atgtataaaa aggctgctaa gccacaaacc agttgtgctg
     3361 tagcagacaa tgcatctgca taattagc
//
LOCUS       DAOPID010000169         3377 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_38898_length_3377_cov_4.780855, whole genome shotgun sequence.
ACCESSION   DAOPID010000169 DAOPID010000000
VERSION     DAOPID010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38898_length_3377_cov_4.780855"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="PK641_05800"
     CDS             <1..601
                     /locus_tag="PK641_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55895.1"
                     /translation="AHNGEVRNYVLRTVIEISDNTNLLSISIEGELIENFDPLVHDYY
                     YTLPFGASFVDVEMLGYEKTEQGQEVTFVKDGMDIQLQVLAQDGTSIGIYTIHFVPDD
                     FDPSVTPTGDEVCITSTDDGGLRFTTKCSNVDIYLSDMSGRMIANYTLPLVDPNIEDI
                     CSPEAEGYVYQGPSGQIVLYYFVYAKKKVIYSGKFRTSN"
     gene            773..1657
                     /gene="fabD"
                     /locus_tag="PK641_05805"
     CDS             773..1657
                     /gene="fabD"
                     /locus_tag="PK641_05805"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HPG55896.1"
                     /translation="MKAYVFPGQGAQFVGMGKNMYESSKVAKDLFEKANDVLGFRITD
                     LMFNGTDEDLRQTKVTQPAVFLHSVILAKTMNEKFQPSMVAGHSLGEFSALVAAGALS
                     FEDGLLLVSKRAKAMQKACELNPSTMAAIIGLPDATVENICAEINGIVVPANYNCPGQ
                     LVISGSVEAVDAACVKLLEAGAKRALKLSVGGAFHSPLMEPAKVELAAAIEDALFSKP
                     ICPVYQNVSTIAEIDPVKIKSNLVKQLTAPVKWTQSVQNIIADGATEFVEVGPGKVLQ
                     GLVIKINKEITAKSIEEL"
     gene            complement(1715..1785)
                     /locus_tag="PK641_05810"
     tRNA            complement(1715..1785)
                     /locus_tag="PK641_05810"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1751..1753),aa:Gln,seq:ctg)
     gene            complement(1860..2870)
                     /gene="gap"
                     /locus_tag="PK641_05815"
     CDS             complement(1860..2870)
                     /gene="gap"
                     /locus_tag="PK641_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008624396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPG55897.1"
                     /translation="MIKVGINGFGRIGRFVFRAAQTRNDIEIVGINDLCPVDYMAYML
                     KYDTMHGQFDGTIEANVEKSQLIVNGKAIRITAERNPADLKWDAVGAEYVVESTGLFL
                     TKEKSQGHIDAGAKYVVMSAPSSDDTPMFVCGVNLDAYKKGTQFVSNASCTTNCLAPI
                     AKVLNDKFGITDGLMTTVHSTTATQKTVDGPSMKDWRGGRAASGNIIPSSTGAAKAVG
                     KVIPSLNGKLTGMSMRVPTLDVSVVDLTVNLAKPAKYSEICAAMKAASEGELKGILGY
                     TEDAVVSSDFLGCKLTSIFDAKAGIALTDTFVKVISWYDNEIGYSNKVLELIAHMAKV
                     NG"
     gene            complement(3004..3129)
                     /locus_tag="PK641_05820"
     CDS             complement(3004..3129)
                     /locus_tag="PK641_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55898.1"
                     /translation="MKKIVLIATLIAALCCLEGCKSPCNCGYALNDLPASIEVNS"
     gene            3257..>3377
                     /locus_tag="PK641_05825"
     CDS             3257..>3377
                     /locus_tag="PK641_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005828284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HPG55899.1"
                     /translation="MQPLAERLRPLDFEEYIGQEHLVGRGAVLRKMIESGNISS"
BASE COUNT         1101 a          548 c          628 g         1100 t
ORIGIN      
        1 tgctcataat ggtgaagttc gtaactatgt attgagaaca gttattgaaa tttctgacaa
       61 tacaaattta ttatcaatat ctatcgaagg agaattgatt gaaaactttg atccattagt
      121 tcacgattat tattatacat tacctttcgg agcatcattt gtggatgttg aaatgctagg
      181 atatgaaaaa acagaacaag gacaagaagt tacctttgtt aaggatggta tggatattca
      241 attgcaagta cttgcacaag acggaacttc aataggtatt tatacaattc actttgtgcc
      301 tgatgatttc gatccatctg taactccaac aggcgatgaa gtatgtatca caagcactga
      361 tgatggagga ttgaggttta ctacaaaatg tagtaatgtt gatatctatc tatcagatat
      421 gtcaggtaga atgatagcta attacacttt accactggtt gatcctaata tcgaagatat
      481 atgtagtcct gaagcagaag gatatgttta tcaaggacct agcggacaaa tagtattata
      541 ttactttgta tacgcaaaaa agaaagtgat atatagcggt aaatttagaa catcaaattg
      601 atgaaataat aacactgaga gtgtggcaag agttatgctt ttgtcacact ttttgtttgt
      661 attggtatca aaaattatat gtaaggggtg tatttgtgaa tggaggtata gactattttc
      721 tacttatgga taaaagtaat acttttgcgg cgttgaatta aatttttaaa atatgaaagc
      781 ttatgttttt cccggacagg gtgctcaatt cgtaggaatg ggcaaaaata tgtacgagtc
      841 atctaaggtt gcaaaagatt tgtttgaaaa ggcaaatgat gtgcttgggt ttagaattac
      901 agatttgatg tttaatggaa cagatgaaga tttacgtcaa actaaggtta cacaacctgc
      961 tgttttcctt cattctgtaa tattagcaaa aactatgaat gaaaaatttc agccatcaat
     1021 ggttgcaggt cattcattag gcgagttttc tgctttagtt gctgcaggag ctttgagttt
     1081 tgaagatggt ttgttactag tttcaaaacg tgcaaaagca atgcaaaaag cttgtgagtt
     1141 gaatccatcc acaatggctg caattattgg attgcctgat gctacagttg agaatatatg
     1201 tgcagaaatt aatggcattg ttgttcctgc aaattacaat tgtcctggac agttagttat
     1261 ttctggatct gttgaagcag ttgatgctgc ttgtgtgaaa ctattagaag ccggtgcaaa
     1321 acgcgcactt aaactatctg ttggtggtgc atttcattca ccacttatgg aacctgctaa
     1381 agttgaatta gcagcagcta ttgaagatgc attattttct aaacctattt gccctgtata
     1441 ccaaaatgta agtacaattg cagaaattga cccggttaag attaaatcaa atttggtaaa
     1501 acaacttact gctcctgtta aatggactca gtcagtacaa aatataatag cagatggtgc
     1561 aacagagttt gttgaagtag gacccggcaa ggtacttcaa ggtttggtta taaaaatcaa
     1621 taaagaaatt actgccaaat ctatcgaaga attataatta cgactagatc ttttatataa
     1681 aaaatgctgt attcatttaa tacagcattt tttgttgccc cactaggact cgaacccaga
     1741 caagcagaac cagaatctga tgtgctacca ttacaccata gggcagaaaa acgggtacaa
     1801 atatatataa aaaatgcgat gttgaaaaga acaccgcatt ttttataata ggatatagat
     1861 tatccgttaa cttttgccat gtgagcaatt aattcaagaa ctttgttaga gtaaccgatt
     1921 tcattgtcat accatgaaat aactttaacg aatgtatctg tcaaagcgat acctgctttt
     1981 gcatcaaaga ttgatgttaa tttgcatcct aagaaatcag atgatacaac tgcatcttcc
     2041 gtataaccaa gaataccctt taattcgcct tctgaagcag ctttcatagc agcgcatatt
     2101 tcgctatatt tagctggttt agccaagtta actgttaagt caactactga tacgtctaag
     2161 gtaggaacac gcattgacat acctgttaat ttgccgttca atgaagggat aactttacct
     2221 acagctttag cagcacctgt tgatgaaggg ataatgttgc ctgatgcagc acgaccacca
     2281 cgccagtctt tcattgaagg accatcaact gttttttgag tagcagttgt tgagtgaact
     2341 gttgtcatca aaccatctgt gataccaaat ttatcattca atactttagc gataggagct
     2401 aaacagtttg ttgtgcaaga tgcattagaa acgaattgtg ttcctttttt gtaagcatcc
     2461 aagttaacac cgcatacaaa cattggagta tcatctgaag aaggagctga cataactaca
     2521 tattttgcac ctgcatcaat gtgtccttgt gatttttctt ttgtcaaaaa taaacctgta
     2581 gattcaacaa catattctgc accaactgca tcccatttca aatcagcagg atttctttca
     2641 gcagtaatac gaattgcttt gccgtttaca ataagttggc ttttttcaac attagcttct
     2701 attgttccgt caaattgtcc gtgcatagta tcatacttta gcatataagc catgtaatca
     2761 accgggcaaa gatcattaat acctacgatt tcaatgtcat ttcttgtttg ggctgcacgg
     2821 aaaacaaaac gtccgatacg tccgaaacca ttaataccta ctttaatcat tgttataaaa
     2881 tttaagttgt taaaatttcg tgcaaaagta cattttttct gcgcgatggc aaaacttttt
     2941 tattactaaa ttagcaaaaa gtataacaaa ttgtcagata ttgcgaatct gactttgtta
     3001 atgttacgaa ttcacttcaa ttgatgccgg taagtcgttt agtgcatatc cgcaattaca
     3061 aggtgatttg caaccttcta aacagcacaa agcagcaatc aatgtggcta ttaatacaat
     3121 ttttttcatg attaatatat ttttgagatg caaagataat gaatttactc gtaaaacaat
     3181 ttgactttag taattttatt attgttgact ttttgtatga taaaaattag tacttttgct
     3241 cattattata atgcaaatgc aaccattagc agaacgtctt agaccattag attttgaaga
     3301 atatatcggg caagaacact tggtcgggag gggtgctgtg ttgcgtaaga tgatagaatc
     3361 aggcaatatc agttctt
//
LOCUS       DAOPID010000170         3363 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_39113_length_3363_cov_4.142382, whole genome shotgun sequence.
ACCESSION   DAOPID010000170 DAOPID010000000
VERSION     DAOPID010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39113_length_3363_cov_4.142382"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PK641_05830"
     CDS             <1..295
                     /locus_tag="PK641_05830"
                     /inference="COORDINATES: protein motif:HMM:NF040284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55900.1"
                     /translation="VQTDAVALTLATTGTNGATVNKWDITANVPAGITGTATTGIGLT
                     SASAIANDTFTNTTAGNLDVVYTVTPYAGDCPGAAYTVTVTVLPDVLAPTIIF"
     gene            359..922
                     /locus_tag="PK641_05835"
     CDS             359..922
                     /locus_tag="PK641_05835"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HPG55901.1"
                     /translation="MLFCLTASVLHAENPDLVESIGRVRNFHVPVIEDADYYWWLDDE
                     LLDENTSEITLEFTESGEFELSAQYSVRGCLSPKSVILISVSDSDIDIPDIPDELPEI
                     YPEKFFSPNGDGNNDTWDIINIQYYPNALIEIYDRFHKRLTQYQGIDKGWDGLYRGHP
                     MPESDYWYIIKDKKLINTRSGHFILKR"
     gene            995..1942
                     /locus_tag="PK641_05840"
     CDS             995..1942
                     /locus_tag="PK641_05840"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HPG55902.1"
                     /translation="MLICALSSCTERIDFTVSDIEPQLVITGYVCTEPGTYAIHLSSS
                     GNFFGKDTLKKYDNAVVSINDYQLENDPNNNGTYITKSDFCAVEGETYTLKVLLDFNL
                     DGVEELYTSTVVAPYTVPLKNLLLQKLSNEEGSSIFPLASLIFFQDPIGPNYYGAHLY
                     LTTARDSNSSYQKYHVSNTPSKYVMNIFSSDVEDGSFIFYPAYLISRRMLHTPLDTLT
                     VYPLDTFELELNNHSQKYFEYLKQASDASSGSNPLFMTPPGVMHGNIEGGALGAFGVY
                     TVSRLKEVMPFAENTWTDEQMILRFGVNWREKFPKEESQ"
     gene            1972..>3363
                     /locus_tag="PK641_05845"
     CDS             1972..>3363
                     /locus_tag="PK641_05845"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPG55903.1"
                     /translation="MLVVINLHAACKLSGGLFDITTGEPLINAYIYVDELQSGTITDI
                     DGNYSFDLENQKKYTIRLSYVGYSSTYQIVKTGNKDKQNQDFYLEQENKHLDEVIVSG
                     ESARKRLEQPTMGVERLEIGQIRKIPAFMGEVDIIKSIQLLPGVQSTSEGSSSFSVRG
                     GAPDQNLILLDGSTIYNASHLMGFFSVFNNDIINDAVLYKGDIPATHGGRLSSLLEIN
                     TKEGNNDKFSGRGGIGLISSRLLLEGPLVKDRLTAWISGRVFYAGMFLPLMKNVNYSL
                     SRTRLTFYDINAKLSATINDKHRLFLSGYMGEDFFALTGTGDFDYANKALSLRWSAIV
                     NEKFYLNTTANCSFYNYLGQGSLSGLNGQWKSSIQDYGLRQEYVWDVDFHNHIKFGFS
                     SSYKYVKTGDASMNQEGIEQDLAISIPPTQSLESALFISNEQKYGQVALSYGLRASIF
                     NNIGPQTELILNEN"
BASE COUNT         1105 a          533 c          640 g         1085 t
ORIGIN      
        1 agttcaaaca gatgcagtag ctctaacttt agctacaacc ggtacaaacg gagctacagt
       61 taataaatgg gatataacag caaatgtacc tgccggaatt acaggtacag caacaaccgg
      121 aatcggttta actagtgcat cagccatagc aaacgataca tttactaata ctacggcagg
      181 aaatttagat gttgtatata ctgtaactcc gtatgccgga gattgcccgg gtgcagctta
      241 tacagtaaca gtaacagtat tacctgacgt attagctcct acaattattt tttagtatta
      301 tatagttttc atcgttttgt ttaaaaggca aattcaaata ttgaatatat tgctgactgt
      361 gctgttttgc ttaacagcct cagttttgca cgcagaaaat cctgatctgg tcgaatcaat
      421 cggccgggtt aggaatttcc atgtgcctgt aattgaagat gctgattatt attggtggct
      481 tgacgatgaa ttattagatg aaaacacatc agaaataact cttgaattta ccgaaagcgg
      541 tgaatttgaa ctatctgctc aatattctgt aagagggtgt ctatcgccca aaagcgttat
      601 tttaattagt gtaagtgata gtgatataga tatcccggat attcccgatg aattacctga
      661 aatttatcct gaaaaattct tcagtcctaa cggagatggg aataatgata cttgggatat
      721 tattaatatt caatactatc cgaatgcact tattgaaata tatgacagat ttcataaacg
      781 cttaactcaa tatcaaggaa tcgataaagg ttgggacgga ttgtatagag gacatccgat
      841 gcctgaatct gattattggt atataataaa agataaaaaa ttaattaata ctcgttctgg
      901 tcactttatt ttaaaacgct aatggcataa aaacactata tttgctgcaa aataattcta
      961 aatgtttaat ttgcgacatt gttttgtgtg ttttatgttg atttgtgcac tttcgtcttg
     1021 cactgagcgt attgatttta ccgtatcaga catagaacct caattagtaa ttacaggata
     1081 tgtatgcacc gaacccggta cttacgcaat acatctgtct tcaagcggta atttcttcgg
     1141 aaaagatact ctgaaaaaat atgataatgc agtagtcagt attaatgatt accaattgga
     1201 aaatgacccg aataacaatg gtacttatat tactaaatca gatttttgtg cagttgaagg
     1261 cgaaacatat actctaaagg tacttcttga ctttaattta gacggagtag aagaactata
     1321 tacatcaaca gtagtagccc cttatacggt ccctctgaaa aatttattat tgcagaagtt
     1381 gtctaacgaa gaaggatcat caatttttcc attggcatca ttgatatttt tccaagatcc
     1441 tataggccct aattactatg gcgctcattt gtatttgaca acagcaagag attcaaactc
     1501 ttcatatcaa aaatatcatg tttcaaatac accctcaaag tatgtgatga acatatttag
     1561 ttcggatgtt gaagatggca gttttatatt ttatcctgca tatttgatat ctagacgaat
     1621 gttacacacc ccattagata cccttaccgt atatccctta gataccttcg aattagaatt
     1681 gaataatcat tctcaaaaat actttgaata tttaaagcaa gcgagtgatg cttcaagtgg
     1741 ttcaaatcct ctttttatga ctcctcccgg tgttatgcac ggaaatattg aaggtggcgc
     1801 attaggtgcg tttggagtct atactgtatc tagattaaaa gaagtaatgc cctttgcaga
     1861 aaatacatgg actgatgaac aaatgattct gcgtttcgga gtcaattggc gtgaaaaatt
     1921 tcctaaggag gaatctcaat gaagcggttt tatatattag ggattttttt tatgctggtt
     1981 gtgattaatt tgcatgcggc atgtaaactt agcggaggtt tatttgatat aactaccggt
     2041 gaaccgctta ttaatgcata tatctatgta gatgaattgc aaagtggtac aatcacagat
     2101 attgacggga attacagctt tgatttagaa aatcagaaaa aatatactat acgtctatct
     2161 tacgttggat attcatctac ttatcagatt gttaagacag gaaataagga taaacaaaat
     2221 caagatttct atttagaaca agaaaataaa catttagatg aagtaatagt ttcgggagag
     2281 agcgcaagga aacgtttgga acagcctaca atgggtgttg agcggcttga aataggacaa
     2341 atccgtaaga tacctgcatt tatgggcgaa gtggatatta ttaaatcaat tcaattatta
     2401 ccgggtgtac aatctacatc agagggctcg tcttcattta gtgtaagagg aggtgcgcct
     2461 gatcaaaatc ttattttatt agacggatca actatataca atgcgtctca tttaatggga
     2521 ttcttttctg tctttaataa cgatattatt aatgatgcag ttttgtataa gggagatatt
     2581 ccggcaactc atgggggaag attatcttca ttgcttgaaa ttaataccaa agaaggtaat
     2641 aacgataagt tttcaggacg aggcggaata ggacttattt cttcaagact tttactcgaa
     2701 ggtccattag ttaaagatag acttactgca tggatttcgg gacgtgtatt ttacgccgga
     2761 atgtttttac cattgatgaa aaacgtcaat tattcacttt ctcgtacacg acttaccttt
     2821 tatgatataa atgcaaaatt atctgcaact ataaacgaca aacatcgttt gtttttgtcg
     2881 ggttacatgg gtgaagactt ttttgcttta acagggaccg gcgattttga ttacgcaaat
     2941 aaggcattat ctttaagatg gagtgccatt gtaaatgaaa aattttacct taataccact
     3001 gcgaattgta gtttctataa ctatttaggt cagggctctt tgtctggttt aaacggacaa
     3061 tggaaatcgt caattcaaga ttatggttta agacaagaat atgtatggga tgttgatttt
     3121 cacaatcaca ttaagtttgg tttttccagc tcttataagt atgtaaaaac gggtgatgca
     3181 agtatgaacc aagagggtat agaacaagat ttagccataa gtattcctcc aacgcaaagt
     3241 ttagaatcgg ccttatttat atcgaatgaa caaaagtatg gacaagtggc attgtcgtat
     3301 ggattaagag catcaatatt taataatata ggacctcaaa ccgaattaat attgaatgaa
     3361 aac
//
LOCUS       DAOPID010000171         3350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_39314_length_3350_cov_4.994234, whole genome shotgun sequence.
ACCESSION   DAOPID010000171 DAOPID010000000
VERSION     DAOPID010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39314_length_3350_cov_4.994234"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1694)
                     /locus_tag="PK641_05850"
     CDS             complement(<1..1694)
                     /locus_tag="PK641_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPG55904.1"
                     /translation="MLFVSSRKGSTGIMRNFRLLFLFVLYCVVTFSASAQLDTTKVYA
                     LAEVQFSPKRQLKEMVPFQTMDAGVIERLSCQSVADAVRYFSGVQIKDYGGVGGIKTI
                     NIRSMGTNQMGVFYDGIQLGNAQNGQIDLGRFSTENMESIDLYNGQRGSVLQSAKDYG
                     SAGTIYLNTRKPVFNDSINYHLRATMKAGSFGLANPSILWEQKLSDKVTLSASAEYTY
                     ANGKYKFRYKKVKNDGTTAYDTTAIRENADINALRTELSLFGKLKEGEWKAQLYNYYS
                     ERGIPGYIARNLYFHKQRQWDDNFFVQTHYKQTFWKKYSILVNAKYAYDYTRYLNPDT
                     TLQYIDDTYKQQEAYLSMANSVNIFPFWDVSLSADYQFNSLDATLRDFAYPTRHTGLV
                     ALATAFKFPNIKLQASVLGTFVHENVKRSIAASDIAVFTPTAIVSVRPWRTIPFDIRA
                     FYKRIFRMPTFNDLYYTFVGNSMLEPEYVTQYDLGLSYHYHDGKHIFSDFGIQLDVYD
                     NQVENKIVAIPSSNPFRWQMKNYGQVRINGIDVSMDFGLQWCCYWNLDLRLAYSYQ"
     regulatory      complement(1706..1900)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(1918..>3350)
                     /locus_tag="PK641_05855"
     CDS             complement(1918..>3350)
                     /locus_tag="PK641_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HPG55905.1"
                     /translation="PQWVYIDLKQSCYISYVTLLWETAYSSQFEIQVSENALDWTTIT
                     TQSGTGGTQTVKIDINARYIRMYGTQRATNYGHSMYEFEVYGIEPQGDTPALFGLDIT
                     PRNMQLKEGESVQLSVKGYDQFGNEMSINPTYLVTSGEGNVTANGLLTPSKYGSITIS
                     ATSEAKSSSVTYLVEEQIKLKQIEISPKTSLLPVGKTQEFTCVATDQFGAEFESSSIG
                     YYLKGDGARLIDNLFYADNVGDYEVSAGVGEIRDTATVSVSSLSDMNLAFNKPVISTG
                     YENAGTLPEYVNDGDITTRWGSQHKDGQWIQFDLKDSYKLTSAEIYWETSSAAEYQLL
                     VSKDGDDWVVAADKKNQPAGSRTDIIDLTQYQGRYLKINCIKRSNTYGFSIFEFRVYG
                     SEIILSAENEEYQTNVEIYAKNGEINIITADKINNIGIYDLTGRCLSFINEIVGNKQV
                     VLDRGHGIYIVRYQQNNVYYQTKIGF"
BASE COUNT         1041 a          684 c          494 g         1131 t
ORIGIN      
        1 tcttgatatg aataggccaa acgcaaatcc aaattccaat aacagcacca ttgtaaacca
       61 aaatccatag aaacatcaat cccgttaata cgaacttgtc catagttttt catctgccac
      121 ctaaatggat ttgaagacgg aatcgcaact attttatttt ctacttggtt atcatataca
      181 tctagctgta ttccgaaatc ggaaaaaata tgtttcccgt catgataatg gtacgaaagg
      241 cctaaatcat attgtgttac atattcaggc tctaacatag aattacctac aaaagtgtaa
      301 tataaatcat taaatgtagg catcctaaat atacgtttat aaaatgctct tatgtcaaat
      361 ggtatcgttc gccatggacg cacagaaact attgctgttg gagtaaatac tgctatgtct
      421 gatgccgcta tagatctctt tacattttcg tgaacaaaag ttcccaatac gctggcttgt
      481 aatttaatat tcggaaactt aaatgcagtg gcaagtgcaa ccaaacctgt atgacgtgtt
      541 ggatatgcaa aatcgcgtaa tgtcgcatct aaactattaa attgataatc agctgacaat
      601 gaaacatccc aaaatggaaa tatattcaca gaatttgcca tcgacaaata tgcttcttgc
      661 tgcttgtaag tatcatcaat atattgcaat gttgtatccg gatttagata tctggtataa
      721 tcataggcat actttgcatt tacaagaatc gaatatttct tccaaaatgt ttgcttataa
      781 tgtgtctgca caaaaaagtt atcatcccac tgtctttgtt tatgaaaata taaatttctg
      841 gctatataac ccgggattcc gcgttctgaa taatagttgt ataattgtgc tttccactct
      901 ccttctttga gttttccaaa taaagacaat tctgttctaa gtgcgttaat atcagcattt
      961 tcgcgaattg cagttgtatc gtaagctgta gtaccatcat tttttacttt tttatatcgg
     1021 aatttatatt ttccgtttgc atatgtatac tctgcacttg cagacaatgt aactttatct
     1081 gataattttt gttcccacaa tatggatgga tttgccaaac cgaaagaccc ggctttcatt
     1141 gttgcacgaa gatgataatt tatagaatca ttaaatacag gcttccttgt attcaagtat
     1201 attgttcctg ccgagccgta atcttttgca gattgtaaca cagaccctct ttgtccattg
     1261 tacaaatcaa ttgattccat gttttctgta gaaaatctgc ctaaatcgat ttgcccattt
     1321 tgagcatttc caagttggat tccatcataa aacacaccca tttgatttgt acccatacta
     1381 cgtatattga tagttttaat accaccaaca ccaccatagt ctttaatttg aactcctgaa
     1441 aaataacgta ccgcatctgc taccgattgg caactaagcc tttcgatgac acccgcatcc
     1501 atagtttgga atggtaccat ctccttaagt tgacgtttag gactaaactg cacttcggct
     1561 aaagcataaa ctttagtagt atctaattga gccgacgcac taaacgttac aacacaatat
     1621 aaaacgaata aaaataaaag acgaaaattc cgcataatac ctgtcgatcc tttcctcgag
     1681 gatacaaaga gcataatagc tcctttctga ttgttatcgt atcggcaggt cttctgactt
     1741 aacccaaaaa tcggcgcctt cccatcctaa taaagacagt ggctaagatt gccgaccaca
     1801 aaggtattac agcagcggga ctgtttggga ttctcaccca attccctctt cgtttactct
     1861 tttatttaaa agagcaaaaa ccaatacggg gcaaagatac atctattttt gtaattatca
     1921 aaagccaatt ttagtttgat aataaacatt gttttgctgg tatctcacaa tatatatacc
     1981 atgtcctcta tccagtacta cttgtttatt acctacaatt tcatttatga aagataaaca
     2041 gcgtccggtc aaatcgtaaa ttccaatatt attaatctta tctgcagtaa ttatatttat
     2101 ttccccgttt tttgcataga tttctacatt cgtctgatac tcttcatttt cagcactcaa
     2161 tataatttca gagccatata ctctaaattc aaatattgaa aagccgtaag tatttgatct
     2221 cttgatacaa ttaatcttca aatatctgcc ttggtattga gtcaaatcta taatgtctgt
     2281 tctacttcct gcaggttgat tctttttatc agctgcaaca acccaatcgt ccccgtcttt
     2341 cgaaaccaat aattgatatt cggcagccga tgaagtttcc caataaattt ccgcgctagt
     2401 taatttataa gaatctttca aatcaaattg aatccattgt ccgtctttat gttgactgcc
     2461 ccatcttgtt gtgatatcac catcatttac atattcaggt aacgttcctg cattttcata
     2521 gcctgttgaa attacaggtt tattaaatgc caaattcata tcgcttaatg aagatacact
     2581 cacagtagct gtatcacgaa tttcgcctac acctgccgac acttcataat cgcctacatt
     2641 atcagcatag aataaattat caataagacg agcaccatct ccttttaaat aatatcctat
     2701 tgatgatgat tcaaattctg cgccgaattg atctgtcgca acacaagtaa attcctgagt
     2761 tttacctacc ggcaacaatg atgttttagg agaaatttct atttgtttca atttgatttg
     2821 ttcctcaacc aaatatgtaa cagaacttga ttttgcttct gatgttgcac taattgtaat
     2881 acttccatat ttactgggag tcaataatcc gtttgcagtt acatttcctt ctccggatgt
     2941 aactaaatat gtaggattaa tagacatttc atttccaaac tgatcatatc ctttaaccga
     3001 taattgaaca gactcacctt cttttaattg catattacga ggtgttatat cgagtccgaa
     3061 caatgccggg gtatctccct gaggctcaat accatagact tcaaattcat acattgaatg
     3121 tccataatta gtagcacgtt gtgttccgta cattctaata taacgtgcat ttatatcaat
     3181 ttttacagtc tgagtacctc ctgttccact ttgagtcgtt atagtagtcc aatcaagtgc
     3241 attttccgaa acttgaatct caaattgtga tgaatatgca gtttcccaca acaatgtaac
     3301 ataactgata taacaacttt gctttaaatc aatatacacc cattgtggat
//
LOCUS       DAOPID010000172         3320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_39827_length_3320_cov_3.345176, whole genome shotgun sequence.
ACCESSION   DAOPID010000172 DAOPID010000000
VERSION     DAOPID010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39827_length_3320_cov_3.345176"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            367..2499
                     /locus_tag="PK641_05860"
     CDS             367..2499
                     /locus_tag="PK641_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HPG55906.1"
                     /translation="MDYRQKISELIADNLQLTVKQVSGTIKLLDDAATIPFISRYRKE
                     MTGGLDEVAIESVKNKFEKYKELIARRVTIVNTIDEQGKMTAELKNRIENCWDSGELE
                     DIYLPYKPKRKTKAEIARQNGLEPLAKILMNQMSIMWDQTIKRFINKEINTTDEALQG
                     ARDIIAEWISENETARNIVRRRFSQDAFITSKVVKAKQEEADNYRDYFDFSEKLSRCS
                     SHRLLAIRRGEKEGFLRVDISPDPERVLEQLKKVFVKANNDSSKQVAIATDDSYKRLL
                     KSSIENEFAVLSKEKADNDAIRVFAENLRQLLMQAPLGQKRVLGIDPGFRSGCKIVCL
                     DAQGNLLHNENIYPHAPQNEISKATSALRKMVESYKIEAIAIGNGTASRETEQFISQI
                     SFDRTLQVFVVSENGASVYSASAVAREEFPDYDVTVRGAVSIGRRLIDPLAELVKIDP
                     KSIGVGQYQHDVDQTQLKNSLTRTVESVVNNVGVDINTASKHLLTYISGLGPVLAQNI
                     VDYRLQNGAFNNRKSLMNVPKMGPKTFEQAAGFLRISKGDNPLDNSSVHPESYSIVEK
                     MASDLSCTVADLMRDAQLRKQINLNKYITDRIGMPTLTDIMHELEKPSRDPRGSVQTF
                     SFDPNVRKIDDLKEGMILPGIVTNITNFGAFVDIGIKENGLVHISQMSDKFISDPNEV
                     LSLHQHLSVRVLSVDMQRKRIALSLKTN"
     gene            2506..3165
                     /locus_tag="PK641_05865"
     CDS             2506..3165
                     /locus_tag="PK641_05865"
                     /inference="COORDINATES: protein motif:HMM:NF014997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HPG55907.1"
                     /translation="MKNCKFYTDREEKANYLTHFIGLIAGMIACFFLIKRAIDANDAW
                     AILAYSIFSFGVLCCMGSSSLYHYISDPKKKAFLRHFDHGAIYIQIATSYAPFTLLLL
                     RNDGFWGWGLFIAVWVAAIIGLIFNCKELKANDNLKTASYVLMGMLVVFAVKPLIDAC
                     IDSNCLNVLYFLALGGFFYVVGAFFYALAKHEFVHAIFHIFVLLGLGCHIVAAFLIPL
                     A"
BASE COUNT         1120 a          482 c          658 g         1060 t
ORIGIN      
        1 ccaaaagtag ttgtaacttg ataggaaatc gcttcgaaat gccaaacgtt tcatacacgc
       61 atccgtaaaa aatagtaatt acttaatgca ataaagctta ttatactttt aatatttagg
      121 ttttaaatag caatatcatc accgtttggc acaaaagatg tgtgatgaga gagaaaaaaa
      181 gaattcataa atcccaaaac agaattcttt aaaatattcg taaattcaca cttatcactc
      241 gaatctatag cgagttgaac aaagacaaaa gcccatattg tgtaattatg ataatgttat
      301 atttgtttag tttgtagcta taattataat cagtaatttt ggtacttcaa attgaaaaaa
      361 ggataagtgg attatagaca aaaaatatct gaattaatag ctgataatct tcaacttaca
      421 gttaaacaag tatcaggaac aataaaactg cttgacgatg ctgcgacaat tccgtttata
      481 agtcgatata ggaaagaaat gacaggtgga ttggatgaag tagctattga gtctgtaaaa
      541 aataaatttg aaaaatataa agaacttata gctcgccgag taactattgt aaacacgata
      601 gatgaacaag gcaaaatgac tgctgagtta aaaaatcgta ttgaaaattg ctgggattca
      661 ggcgaacttg aggatatata tcttccctac aaacctaaac gtaaaacgaa ggccgaaatt
      721 gctcgtcaaa acggcttgga gccattagca aagattctga tgaatcaaat gtctataatg
      781 tgggaccaaa ccattaaacg ctttatcaac aaagaaatta atactactga tgaggctttg
      841 caaggagctc gcgatattat tgcagagtgg attagtgaaa acgaaacagc aagaaatatt
      901 gtaagacgca gattttctca agatgcattt attacttcaa aagtagttaa agcaaagcaa
      961 gaagaagctg ataattacag agattatttt gatttctcgg agaaattatc tcgttgctct
     1021 tcacatcgtt tattggcaat acgcagaggc gaaaaagaag gatttttaag agtcgatatt
     1081 tcgcctgatc ctgaaagagt attagaacaa ttaaaaaagg tatttgtaaa agcaaataat
     1141 gattcttcaa aacaagtggc tattgctaca gatgatagtt acaagcgttt gttaaaatcg
     1201 tcgatcgaaa acgaatttgc agtattgtca aaagaaaagg ctgataatga tgcaattagg
     1261 gtttttgcag aaaatttacg tcaattattg atgcaggcgc ctcttggtca gaaaagagtt
     1321 ttaggtatag atcccggttt tagaagtgga tgcaaaatag tatgtttgga tgcgcaggga
     1381 aatttattgc ataatgaaaa tatatatccc catgctcctc aaaatgaaat aagcaaagct
     1441 acatctgctt tacgaaaaat ggttgaatct tataaaatag aggccattgc tatagggaat
     1501 ggtacagcga gtagggaaac ggaacaattt attagtcaaa tttcgtttga tcgcactttg
     1561 caggtatttg tagttagtga gaatggtgca tctgtatatt ctgcatccgc agtagcaaga
     1621 gaagaatttc ctgattatga cgttaccgta cgtggtgcag tttctattgg caggagacta
     1681 attgatccgc tggcagaact tgttaagata gatcctaaat ctataggcgt aggtcaatac
     1741 caacatgatg tagatcaaac gcaacttaaa aattcactta caagaacagt tgaaagtgtt
     1801 gttaataatg taggtgtaga tattaatact gcaagtaagc atttacttac atatatttca
     1861 ggattaggtc ctgtgctggc tcaaaatatt gtagattaca gattacagaa tggtgctttt
     1921 aataatcgca agagtttaat gaatgtgcct aaaatgggac cgaaaacatt tgaacaggca
     1981 gcaggttttt tacgaatcag taagggtgat aatcccttgg ataattcttc tgtgcatcct
     2041 gaatcttata gtatagttga gaaaatggca tccgatttga gttgtacggt tgccgatttg
     2101 atgcgtgatg ctcaattgcg caagcagatt aatctaaata aatatattac cgacagaata
     2161 ggtatgccca cattgactga cattatgcat gaattagaaa agccaagcag agatccgcgt
     2221 ggaagcgtac agacattttc ttttgatcct aatgttagaa aaatagatga tttaaaagaa
     2281 gggatgattt taccgggaat agtcaccaac ataactaatt ttggtgcatt tgtagatata
     2341 ggtataaaag aaaatggatt ggttcatata tctcaaatgt cagataaatt tatttccgac
     2401 cccaatgagg ttttaagctt gcatcaacat ttatctgtaa gagtcttgtc ggtagatatg
     2461 cagcgcaagc gcatagcatt aagtttgaaa actaattaat acaatatgaa aaactgtaag
     2521 ttttatacag atcgtgaaga gaaggctaat taccttactc actttatagg tttaatagct
     2581 ggaatgatag cttgcttttt tcttataaaa agggcaatag atgctaatga tgcttgggct
     2641 atacttgctt attcgatatt tagttttggt gtactttgtt gtatggggtc ttcgtctttg
     2701 tatcattata ttagtgatcc caagaaaaaa gcatttttgc gtcattttga ccatggtgct
     2761 atatatattc aaattgctac aagttatgca ccgtttactt tgttactact cagaaatgat
     2821 ggattttggg gatggggatt atttatagca gtatgggtag ctgctattat aggattgatt
     2881 tttaattgca aggaacttaa agcaaacgac aatttaaaga ctgcctctta tgttttaatg
     2941 ggcatgctgg ttgtttttgc tgtcaaacct ttgattgatg cctgtattga ttcaaattgt
     3001 ttgaatgtct tgtatttctt agctttagga ggatttttct atgttgtagg ggcattcttc
     3061 tatgctttag caaaacacga attcgtgcat gcaatatttc atatttttgt gctccttggc
     3121 ttaggatgcc atatagtagc ggccttttta atccctcttg cctgaaactt tttccttcga
     3181 gacgataatg taggtattgc tatttagagg agcccatatt gtgtgagtat cccatagttt
     3241 tttaaaatct ttaatagaga taactctgtt atataaagtg ctatccttgc tatttgttaa
     3301 gtaatcaagt gattctgcca
//
LOCUS       DAOPID010000173         3316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_39883_length_3316_cov_4.026066, whole genome shotgun sequence.
ACCESSION   DAOPID010000173 DAOPID010000000
VERSION     DAOPID010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39883_length_3316_cov_4.026066"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /gene="pyrH"
                     /locus_tag="PK641_05870"
     CDS             <1..589
                     /gene="pyrH"
                     /locus_tag="PK641_05870"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPG55908.1"
                     /translation="SMGVQIAIVIGGGNIFRGLIGAAKGFDRVKGDQMGMLATVINSM
                     ALSSALDALNVKNRVLTAIGMEPIGELYSSSKAIKCIEAGEIVIVSGGTGNPFFTTDT
                     ASALRGIEVKADLMLKGTRVDGVYTTDPEKDPKAEKFSEITFDEIYNRNLKIMDLTAT
                     TLCKENRLPIYVFNMDVYGNLKRVISGEKIGTLVK"
     gene            607..1167
                     /gene="frr"
                     /locus_tag="PK641_05875"
     CDS             607..1167
                     /gene="frr"
                     /locus_tag="PK641_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPG55909.1"
                     /translation="MLDVQKNLKDAIEKMENSMFFLDESLAHIRAGKANPRILDGVKV
                     EYYGTLTPLSNVANINTPDAKSIVVQPWEKSMLREIEKAILASEVGITPENNGEVIRL
                     GIPPLTEERRRNLVKQSKQEGEDAKISIRTARREAIDNLKKSVKEGMPEDVEKDAESE
                     IQKVHDKYIKQVDEIVFEKEKEIMTV"
     gene            1177..2301
                     /locus_tag="PK641_05880"
     CDS             1177..2301
                     /locus_tag="PK641_05880"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPG55910.1"
                     /translation="MRVFLFVLTALLATQVVYSSNDSIQHTESDTLTQVYCPVVKKSW
                     FAVPIVFYQEETSAGVGASGGYYFKSQNLKKISSISGSIIYTFLNQAKININPRFYTP
                     KEKFYFTGNLNLRYYPELFFGIGNDALDESVKYTSEAFSLNFQPFYAVTPNFHVGLYL
                     SCLGEKLKLDEETEKKRPELIEKYGKAGWDPYFMWAFGAQLMYDTRDNQFYPQKFSNF
                     IKLSAFTNLKALGSSYDVTSLTLDIRQYIPTWVGQVFAWQIYADARFGKEIPFRMLPT
                     LGGSDNLRGFRERKFTDNFMFEFQAEYRIPIWWRLKAAVFCSVGDVIDIYDPHIQKIK
                     VGYGLGLRCRLNDARVHLRVDVAGNNYGEFKFYITATEAF"
     gene            2303..3211
                     /gene="rsgA"
                     /locus_tag="PK641_05885"
     CDS             2303..3211
                     /gene="rsgA"
                     /locus_tag="PK641_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HPG55911.1"
                     /translation="MQFGLVLRTTGSWYEICTDNGDMVSSRIKGKFRLQGIRTTNPIA
                     VGDRVGIDGDSIVEICPRVNYMIRKSSNLSKKSQIIAANIDQAFLMVSVREPETPAEF
                     IDRFLVTAEAYKIPVTIVFNKIDLYNTTDKEMLNAYMRLYSILGYHCHAVSVKTGEGI
                     EEIKEKLRNKITLFSGNSGVGKSSFINILHPSFNTKTAAISEVHHTGMHTTTFSEMLP
                     YNGGYVIDTPGVKGFGVIDMVAGEVGHYFPEIFSASKECRFANCIHKDEPGCAVIKAV
                     EKHYISKSRYASYLSILEDESENKYR"
BASE COUNT         1123 a          454 c          695 g         1044 t
ORIGIN      
        1 ttcaatgggg gtgcagatag caatagttat tggtggcgga aatatattta gaggtttgat
       61 tggtgctgca aaaggatttg atcgtgttaa aggagatcag atgggaatgt tggctactgt
      121 gattaatagt atggcactaa gctctgcttt agatgctttg aatgtaaaga atagggtact
      181 tacagctata ggtatggaac ctataggtga attatatagt agttctaagg caataaaatg
      241 tattgaagca ggtgagattg taattgtatc aggaggtaca ggaaatccat tctttactac
      301 tgatacagct tctgccttgc gtggtataga agtgaaagct gatttgatgc taaaaggtac
      361 tcgtgttgat ggtgtttata caacagatcc ggaaaaagat cctaaagcag aaaaattctc
      421 tgagataact ttcgatgaga tatacaacag aaatttgaag attatggatt taacggcgac
      481 tacgctgtgt aaagaaaaca gactgccaat atatgtattt aatatggatg tgtatggtaa
      541 tttgaaacgt gtcatctctg gtgaaaaaat aggaacttta gtaaaataac aataatatat
      601 acaacaatgc ttgatgttca gaaaaattta aaagatgcta tcgagaaaat ggaaaattca
      661 atgttctttt tggatgagtc tcttgcacac atacgtgccg gtaaggcaaa tccaagaatc
      721 ttagatggcg tgaaagttga atattatgga actctgactc ctcttagtaa tgtagctaat
      781 ataaacacac ctgatgctaa atcgatagta gtacaaccat gggaaaaatc gatgcttcgt
      841 gaaattgaaa aagcaatttt agcatcagag gttggtatta ctccagagaa taatggtgaa
      901 gttatccgtt taggaatacc tccgttaaca gaagaaagac gtcgtaattt agtaaaacaa
      961 tctaaacaag agggtgaaga tgctaagatt agtatacgta cagcacgtag agaggctatt
     1021 gataatctta agaaatcagt aaaagaagga atgcctgagg atgttgaaaa agatgctgaa
     1081 tctgaaattc agaaagtaca cgataaatat attaaacaag ttgacgagat agtctttgaa
     1141 aaagaaaaag agattatgac ggtataactt ccttgtatga gggtatttct tttcgtttta
     1201 actgcgctcc ttgcgacaca agttgtatat tcttcaaatg attcaataca acatactgaa
     1261 tcagacactt taacgcaagt ttattgtccc gttgtaaaaa agagttggtt cgctgtgccg
     1321 attgtttttt atcaagagga aacaagtgca ggtgtaggtg cttcaggagg ttattatttt
     1381 aaaagtcaaa atcttaaaaa gataagtagt atttcgggaa gtattattta cacattttta
     1441 aatcaagcta aaattaatat taatccacgc ttttatacac ctaaagaaaa attttatttt
     1501 acggggaatt tgaacttacg ttattatccc gaattgtttt ttggaatagg taatgatgct
     1561 cttgatgagt ctgttaaata cacgtctgag gcttttagtt tgaattttca gccattctat
     1621 gcggttactc ccaattttca tgtgggttta tatttgtctt gtttagggga aaaattgaaa
     1681 ttggacgagg aaacagagaa aaagagacct gaattaatag aaaagtatgg aaaagcagga
     1741 tgggatcctt attttatgtg ggcttttgga gctcagttga tgtatgatac tcgtgataat
     1801 caattctatc cacagaaatt tagcaatttc ataaaattgt cagcatttac caatttaaaa
     1861 gctttaggta gtagttatga cgttacttcg cttacattag acataagaca atatattcca
     1921 acatgggttg gtcaagtttt tgcatggcaa atatatgcag atgcgcgttt tggaaaagaa
     1981 attccttttc gtatgttgcc gaccttaggt ggcagtgata atttacgcgg tttcagggaa
     2041 cgtaaattca ccgataattt tatgttcgaa tttcaagcag agtatagaat tcctatttgg
     2101 tggaggttaa aagctgcagt attttgctct gttggagatg taatagatat atatgatcct
     2161 catatacaaa agataaaagt cggatacggc ttaggtctaa ggtgtagatt gaatgacgcg
     2221 agggttcatt taagggtcga tgtggccgga aataattatg gcgaatttaa gttttatata
     2281 acagcaacag aggctttcta atatgcaatt cggattggtt ctacgaacaa caggtagttg
     2341 gtatgagatt tgtaccgata atggtgacat ggtttcatct cgtatcaagg gtaaatttcg
     2401 tttacaaggg attcgtacaa ccaatcccat agcagtgggt gatagggtag gtatagatgg
     2461 cgatagtatt gtagaaattt gtccacgtgt aaattatatg atacgtaaat cgtccaactt
     2521 atcaaaaaaa tcgcaaatta tagcggcaaa tattgatcag gcttttttaa tggtttctgt
     2581 acgtgagcct gaaacccccg cagaatttat agatcgtttt ttagttaccg cagaagccta
     2641 taaaataccg gttactatag tttttaataa aatcgatttg tataatacta ctgacaagga
     2701 aatgctgaat gcgtatatgc gtttatattc tattttagga tatcactgcc atgcggtttc
     2761 tgttaaaaca ggtgagggaa ttgaagaaat aaaagaaaag cttcgtaata aaataacatt
     2821 attttcaggt aattcaggtg taggtaaatc atcatttata aatattctcc atccttcttt
     2881 taatacaaaa acagcagcta tatctgaagt tcatcacaca ggaatgcata caacaacttt
     2941 ttcagagatg ctaccatata atggtggata tgtaatagat actcctgggg taaaaggctt
     3001 cggagtaata gatatggttg cgggagaggt tggacactat ttcccggaaa ttttttcagc
     3061 ttcaaaggaa tgcagatttg ctaattgtat acataaagat gaacccggct gtgcagtgat
     3121 aaaggccgta gaaaaacatt atatcagcaa gtcaagatat gcaagctact taagcatatt
     3181 ggaagatgaa agtgaaaata aatatagata gttttaataa taaacttact tatctttcgt
     3241 tgtattatta atggcatcat caatttacga tattcaacaa tacactaagg ctgtatttat
     3301 agttcttgca gtgata
//
LOCUS       DAOPID010000174         3299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_40164_length_3299_cov_3.362207, whole genome shotgun sequence.
ACCESSION   DAOPID010000174 DAOPID010000000
VERSION     DAOPID010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40164_length_3299_cov_3.362207"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1299
                     /locus_tag="PK641_05890"
     CDS             <1..1299
                     /locus_tag="PK641_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HPG55912.1"
                     /translation="EVLGFVDGKTSLEWIQNAGCKVLNLLCKGSENHCKYQLRKTPEE
                     HLKDIINVLNDADEMGISVNVYLEDWSNGIQNSPDYVYMLVDELRGHNIKHFMLPDTL
                     GILNPTQVSDYCTLMLNRYPELSFDFHPHNDYDLAVANVYAAVKAGIQCVHVTVNGLG
                     ERAGNAPMSSVLAVIHDQLHLQTNINEREINRVSKIIETYSGIRIPNNKPIIGENVFT
                     QVAGVHADGDAKNKLYYNDLLPERFGRTREYALGKTSGKANIRKNLESLGIDLDEESM
                     KKVTSRIIELGDKKQQVTPEDLPYIVSDILKNENLEEKVKLNNYSLSLTRGLKPVANV
                     SVEIDGKVHQESASGDGQYDAFMKALWSIYDKLGKQRPILFDYAVTIPPGGHTDAFVQ
                     AIITWNMNGKIFKTHGLDADQTEAAIKATIKMLNIIEKYQ"
     gene            1330..1797
                     /locus_tag="PK641_05895"
     CDS             1330..1797
                     /locus_tag="PK641_05895"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HPG55913.1"
                     /translation="MKKLTIALVAHDQRKVDMVEWAEYNVDLLKQHKLVCTGTTGYLI
                     QKALESKGAVVDITRMHSGPLGGDAEVAAMVVRHEIDLAIFLIDDLNPQPHEADIQML
                     LRQCRVHNVPIACNRYSADLMITSSLWDDQDYKPSIPKYISFDREEFDIKHSK"
     gene            1805..2866
                     /gene="leuB"
                     /locus_tag="PK641_05900"
     CDS             1805..2866
                     /gene="leuB"
                     /locus_tag="PK641_05900"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HPG55914.1"
                     /translation="MNFKIAVLPGDGIGPEISIQGVNVMTAVCKKFGHNVSYEYGICG
                     ADAIDKVGDPFPAETLNICKNADAVLFSAVGDPKFDNNPSAKVRPEQGLLAMRKQLGL
                     FANIRPVQTFKCLIHHSPLKPELVEGADFICIRELTGGMYFGEKYQDNDKAYDTNLYT
                     RPEIERILKVGFEYSMKRQKHLTVVDKANVLASSRLWRQIAQEMEPSYPEVKVDYMYV
                     DNAAMRMILSPTFFDVMVTENTFGDILTDEGSVISGSMGLLPSASTGESTPVFEPIHG
                     SWPQAKGLNIANPLAQILSVAMLFEYFDLKEEGKLIRKAVDCSLESNVRTPEIQIESY
                     PKYGTKEVGEWIVDYINKQ"
     gene            complement(2887..3111)
                     /locus_tag="PK641_05905"
     CDS             complement(2887..3111)
                     /locus_tag="PK641_05905"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HPG55915.1"
                     /translation="MSDTDRTIVIKNYDNLTLAHYEKSVIEDAGIDCFISGDNMAQPF
                     TMFAPIGGIKLHIYEKDAEKALEILNSLGK"
     gene            complement(3114..>3299)
                     /locus_tag="PK641_05910"
     CDS             complement(3114..>3299)
                     /locus_tag="PK641_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPG55916.1"
                     /translation="PDETGVFLETAHPAKFKDTVENILGTQIQIPAKLQEFMKGTKQS
                     IYLSKDFEDFKSFLFKE"
BASE COUNT         1128 a          469 c          642 g         1060 t
ORIGIN      
        1 gaagtattag gatttgtaga tggtaagaca tcgctcgagt ggatacaaaa tgctggttgt
       61 aaggtgttga atttactttg taaaggatct gagaatcatt gtaaatatca gttgagaaaa
      121 acaccggaag aacatttgaa agacattatt aatgtcctta atgacgccga tgaaatgggc
      181 atatctgtaa atgtttattt agaagactgg agtaatggaa ttcaaaacag cccggattat
      241 gtatatatgt tagttgatga attgagagga cataatatta agcatttcat gcttcctgat
      301 acattaggaa ttcttaatcc tactcaagta agcgattatt gtacattgat gctgaatcgt
      361 tatcctgaat tgtcttttga ttttcatcct cataacgatt acgatttagc tgtagcaaat
      421 gtatatgcgg cagttaaagc aggaattcaa tgtgttcacg taactgtcaa tggattaggc
      481 gagagggcag gaaatgcacc catgtctagt gtattagctg ttattcatga tcaattacat
      541 ttgcaaacta atataaacga gcgtgaaata aatagggtaa gtaaaattat agaaacttat
      601 tcgggcatta gaattccgaa taataagcct ataattggtg aaaatgtatt tactcaagta
      661 gccggagttc atgctgacgg agacgctaaa aataaattat actataacga tttattacct
      721 gaaagatttg gtcgtacacg agaatatgca ttaggtaaaa cttcaggtaa agcaaacatc
      781 agaaaaaatc ttgagtcttt gggcattgat ttggatgaag aatcaatgaa aaaagtaaca
      841 tcacgtatta ttgagttagg agataagaaa caacaggtaa ctcctgaaga tcttccttat
      901 attgtttctg atatacttaa gaacgaaaat ttggaagaaa aagtcaaatt aaataactat
      961 tctttatctt tgacacgagg tttgaaacct gttgccaatg tttctgttga aattgatgga
     1021 aaagtacatc aagaatctgc atccggagat ggacaatatg atgcatttat gaaagcattg
     1081 tggagtatat acgataagct tggtaaacag cgtcctattt tgtttgacta tgctgttaca
     1141 attcctccgg gaggtcatac agatgccttt gtacaagcca taattacatg gaatatgaat
     1201 ggtaaaatat ttaaaacaca tggcttagat gcagaccaaa cagaggcagc tattaaagct
     1261 acaataaaga tgttgaatat tattgagaaa tatcagtaaa cagattcatc ttaactaaaa
     1321 cgaaaaaata tgaaaaaact aactattgct ttggttgcgc atgatcaacg taaagttgat
     1381 atggtagagt gggcagagta taatgttgat ttgctaaagc aacataaatt agtttgcaca
     1441 ggaaccaccg gttatttgat acaaaaagca cttgaatcta aaggagctgt tgttgatata
     1501 acacgtatgc attcaggtcc attaggagga gatgcagagg tagcagctat ggttgtaaga
     1561 catgaaatag atttagcaat attcttgatt gatgacttaa atccacaacc tcacgaggct
     1621 gatattcaaa tgttattgcg tcagtgtaga gttcataacg tacctattgc atgtaataga
     1681 tatagtgcag atttaatgat aacaagtagt ttgtgggatg atcaagatta taaaccatca
     1741 atccctaaat atatctcatt tgatcgcgaa gaatttgata taaaacattc aaaataatta
     1801 aaaaatgaat tttaaaatag ctgtattgcc gggtgatggc ataggtcctg aaatatctat
     1861 tcagggtgtt aatgtaatga ctgcagtatg taaaaagttt ggacataatg taagttatga
     1921 gtatggaata tgtggtgctg atgcaattga taaagtagga gatccctttc ctgcagaaac
     1981 tttaaatatt tgtaaaaatg ctgatgctgt attattttcg gcagtaggtg atcctaaatt
     2041 tgataataat ccatcggcaa aagtgcgtcc ggaacaaggt ttattggcta tgcgcaagca
     2101 attaggactt tttgcaaata ttcgtccggt gcaaacattt aagtgtctga tacaccattc
     2161 tccattaaaa cctgaattag tagaaggtgc agattttatt tgtattagag aattgacagg
     2221 aggtatgtat tttggagaga agtatcaaga taatgataaa gcatatgata ctaatcttta
     2281 tacacgtcct gaaatagaac gtattttgaa agtaggcttt gaatattcaa tgaaacgcca
     2341 aaaacatctg acagtagttg ataaagctaa tgtattggca tctagccgtt tatggagaca
     2401 aattgctcaa gaaatggaac catcatatcc tgaagttaaa gtagattata tgtatgttga
     2461 caatgctgca atgcgtatga ttttatctcc aacttttttt gatgtaatgg ttacagaaaa
     2521 tactttcgga gatatattaa cagacgaagg ttctgttatt tccggttcaa tgggattgct
     2581 gccatctgca tccacaggtg aaagtactcc tgtttttgaa ccgatacacg gatcatggcc
     2641 gcaggctaaa ggtttaaata ttgcaaatcc tttggcgcaa atattgtcag tagctatgct
     2701 atttgaatat tttgatttaa aagaagaagg taaattgatt cgtaaagcag ttgattgtag
     2761 tttggaatct aatgttagaa ctcctgaaat tcaaattgaa tcatatccaa aatatggaac
     2821 aaaagaagta ggcgagtgga ttgtagatta tatcaacaag caatagcatt aaaaaatagc
     2881 cttggtttat ttaccaaggc tatttaatat ttctaatgct ttttcagcgt ctttttcgta
     2941 tatatgtaat ttgatacctc caattggagc aaacatggta aagggttgcg ccatattatc
     3001 tccacttata aaacaatcaa ttccggcatc ttctattaca cttttttcgt aatgtgctaa
     3061 tgttaaatta tcatagtttt taatgactat tgtcctatcg gtatcgctca tatttattct
     3121 ttaaaaagga atgatttgaa atcttcgaaa tccttactca aatatatact ttgttttgtc
     3181 cctttcatga attcttgcaa ttttgcagga atttgaattt gagttcctag tatattttca
     3241 accgtatctt taaattttgc aggatgagca gtttctaaga aaacacccgt ttcatctgg
//
LOCUS       DAOPID010000175         3286 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_40372_length_3286_cov_4.052925, whole genome shotgun sequence.
ACCESSION   DAOPID010000175 DAOPID010000000
VERSION     DAOPID010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40372_length_3286_cov_4.052925"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="PK641_05915"
     CDS             complement(<1..685)
                     /locus_tag="PK641_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPG55917.1"
                     /translation="MNEISLLNRAADNIRILAASMVEKAKSGHPGGAMGGADFINVLY
                     SEFLVYDYSDPNWENRDRFFLDPGHMSPMLYSVLSLAGKYTIDDLKSFRQWGSITPGH
                     PEVDLIHGIENTSGPLGQGHTMAVGAAIAERFMAARFGEWTKHKIYTFISDGGIQEEV
                     SQGAGRIAGLLGLSNLIMFYDSNDIQLSTFVNQVTNEDTAKKYESWGWNVITINGNDA
                     SQIRRALQSA"
     gene            complement(885..1637)
                     /locus_tag="PK641_05920"
     CDS             complement(885..1637)
                     /locus_tag="PK641_05920"
                     /inference="COORDINATES: protein motif:HMM:NF039966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6048 family protein"
                     /protein_id="HPG55918.1"
                     /translation="MTSILKYFFSFSLLLWATSIYAQSSQTTVNDSAAQRRVQLDVMN
                     KVNKNKYIYQGAYLNLDIYDPIATLFNGGRFQFEVSADVSLWQRLFPAIDFGMMFMDQ
                     KNTDYRYESNGFFFRVGANYNFINFKDTRRYDHILAVGVRYGYAHLGYKLSDAVCTNT
                     YWNESTSAETEWKPVDTGWVEFLATVRAQIYKSFFMGLTIRVQTFQHFYQASTDYPSY
                     IPGYGVYGSSSTNWGVDFTLCYQFPYKKKPTL"
     gene            complement(1609..2091)
                     /locus_tag="PK641_05925"
     CDS             complement(1609..2091)
                     /locus_tag="PK641_05925"
                     /inference="COORDINATES: protein motif:HMM:NF040109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6452 family protein"
                     /protein_id="HPG55919.1"
                     /translation="MKLKTIYCIFSAIIIAVCMASCSGDCYVPTKARMGVAFMDSTTL
                     KPYTINGLTVQGVGSDSILYNKVNTSVIYLPLHDNAEQTDFKITIASTVEGEQDKEYI
                     MHIDHTPRPQLISPECGCVMFQTVISADVSPVMSDYTIEIYNAEVKNIENDIHLKIFF
                     "
     gene            complement(2096..2620)
                     /locus_tag="PK641_05930"
     CDS             complement(2096..2620)
                     /locus_tag="PK641_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55920.1"
                     /translation="MDAFFEILKYTIPALIVFAVVYLMLRKYMQVEYSRRKYLLYKES
                     QKTTLPIRLTAYERIVLFLERISPDSLLVRIQDGSMNSLQFHAALLNAIRSEYEHNVA
                     QQVYISEEAWNVVKNSKENIVQLINACAAQVSPQDPSYDLAKLILSTYEANSDSPTMA
                     AIAFLKNEIKNYFT"
     gene            complement(2624..>3286)
                     /locus_tag="PK641_05935"
     CDS             complement(2624..>3286)
                     /locus_tag="PK641_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPG55921.1"
                     /translation="PEEIPGLYNMIKKEGYDLVSGWKKKRYDPKLTKNLPSKLFNATA
                     RKVSGIYLHDFNCGLKAYRSEVVKSIEVYGEMHRYIPYLAKNAGFSRIGEKEVNHQMR
                     KFGKSKFGFSRFVNGYLDLITLWFLSKFGVKPMHFFGVWGSIMFLFGLISVIIVGVMK
                     LNAIIQGLRAPLITSNPYFYLAILAMILGTQLFLTGFLGELISRSSQERNNYQIAENL
                     NL"
BASE COUNT         1108 a          632 c          497 g         1049 t
ORIGIN      
        1 tagcagactg taacgctcta cgtatttgag aagcatcatt accatttatt gtaataacat
       61 tccagcccca tgattcgtat tttttagctg tatcttcatt tgttacttga ttaacaaatg
      121 ttgaaagctg tatatcattt gaatcataga acataatcaa gtttgataaa cctaacaaac
      181 cggctatacg tcccgctcct tgcgatactt cttcttgaat accaccatca gagataaaag
      241 tatatatttt atgttttgtc cattctccaa acctagcagc cataaaacgc tctgcaatag
      301 cggcacctac tgccattgtg tgtccttgac caagtggacc tgatgtattt tcaattccat
      361 gtataaggtc tacttcagga tgtcccggag ttatacttcc ccactgacga aatgacttca
      421 aatcatctat cgtatattta cctgccaaag acaaaacaga atacaacata ggcgacatgt
      481 gacccggatc taaaaagaag cgatctctat tctcccagtt tggatcggaa taatcatata
      541 ccaaaaattc ggaatataat acattgataa aatcagcacc acccatggct ccaccaggat
      601 gtccggactt tgctttttca accattgatg ccgccagaat gcgaatgtta tctgcagctc
      661 tgttaagtaa ggatatctcg ttcatgaaac taaaattgta atctatagat tgcacaaagg
      721 tacaaaaata cggtgagcct acctcatatc atagcaagat atattgttac aacacagaaa
      781 aagatttcaa caattttaag taaatactta aaaatgaaga cataaaaaat ctcaatgtat
      841 attatgcatc tatcaaaaga aaaagattat atcacccgaa aaaattacag tgtaggtttc
      901 tttttatatg ggaattgata acaaagtgta aaatcaaccc cccagttagt ggaagaagag
      961 ccataaacgc cgtatcccgg tatatatgaa ggataatcag tagatgcttg ataaaagtgt
     1021 tggaatgttt gaacgcgtat cgtcaagccc ataaaaaagc ttttatatat ttgagcacga
     1081 actgttgcca aaaattcaac ccaacctgta tcaaccggtt tccattccgt ttcggcagat
     1141 gtactctcat tccaataagt atttgtacat acagcatcag ataatttata accaaggtgt
     1201 gcataaccat atcgcactcc gacggctaaa atatgatcgt aacgccttgt atctttaaaa
     1261 ttgataaaat tataattagc cccgacgcgg aagaaaaatc cattagattc gtaacgataa
     1321 tcagtgtttt tttgatccat aaacatcatt ccgaaatcta ttgcagggaa taaacgttgc
     1381 cataatgata catctgctga aacctcaaat tggaatcgtc ctccattaaa cagagtcgct
     1441 attgggtcat aaatatccaa attcaggtat gcaccttgat atatatattt attcttgttg
     1501 actttattca taacatccaa ttgcacacgc ctctgtgcag cagaatcatt cacagtagtt
     1561 tgtgatgact gtgcataaat agaagtagcc caaagcaata atgagaaact aaaaaaatat
     1621 tttaagatgg atgtcatttt ctatgttttt aacttcagca ttataaattt ctatagtata
     1681 atcactcatc accggtgata catcagcaga tattactgtc tgaaacataa cacaaccgca
     1741 ttccggactt attaattgag gtctgggagt atgatcaata tgcatgatat actctttatc
     1801 ctgttcacct tcaactgttg atgctatagt aattttaaaa tcagtctgtt ctgcattatc
     1861 atgcaatggt aaatatataa cagaagtatt aactttgttg tatagaatag aatcggagcc
     1921 aacaccttgc actgttaatc cattaattgt ataaggcttc agtgtagttg aatccataaa
     1981 tgcaaccccc attcttgctt ttgtaggcac atagcaatcg ccactacaag acgccatgca
     2041 gactgcaatt attattgcag aaaatataca atatatcgtt ttgagtttca ttaaactatg
     2101 taaaataatt ctttatctca tttttaagaa aagctatagc agccatagtt ggagaatctg
     2161 aatttgcctc gtatgttgaa agtatcaatt ttgcaagatc gtatgaagga tcttgtggag
     2221 atacctgagc tgcacatgca ttaatcaatt gaacaatatt ttcttttgaa tttttaacga
     2281 cattccaagc ttcttcgcta atataaacct gctgtgctac attatgctca tattcagagc
     2341 gtattgcatt aagtaaagct gcatgaaact gcaaactatt cattgaacca tcttgaatac
     2401 gtacaagtaa agaatcagga cttattcgct ctaggaacaa aacaattctt tcgtaggcag
     2461 taagacgtat tggaagagtc gttttctgag attccttata tagtaaatac ttgcgtctgg
     2521 aatattctac ttgcatatat ttgcgtaaca tcaaatatac aactgcaaaa acaattagtg
     2581 caggaatagt gtatttcaat atttcaaaaa aagcatccat ttactataaa tttaagtttt
     2641 cagctatttg ataattattt cgctcttgag atgatctact tattaattct cctaaaaatc
     2701 cggtcaaaaa caattgtgtt ccaagtatca tcgctaatat agctaagtaa aaatatgggt
     2761 tacttgtaat aagcggtgct cttaatcctt gtattattgc atttaatttc attacgccaa
     2821 caataatcac tgaaattaaa ccaaataaga acattatcga cccccatact ccaaaaaaat
     2881 gcatcggctt aactccgaat tttgataaaa accaaagagt tattaaatcc aagtaaccat
     2941 ttacgaaacg actgaagcca aatttacttt tgccgaattt acgcatttga tgatttactt
     3001 ctttctcgcc tattctagaa aatccggcat tctttgcaag atatggaatg tatctatgca
     3061 tttctccgta aacctcgata ctcttaacaa cctcagatct atatgctttt aaaccacaat
     3121 taaaatcatg taaataaata cctgaaactt ttcttgcagt tgcattaaac aacttactag
     3181 gtagattttt cgttaacttt ggatcatatc ttttcttttt ccagcctgat accaaatcat
     3241 agccttcttt tttaatcata ttgtacaatc cgggaatttc ttcagg
//
LOCUS       DAOPID010000176         3282 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_40429_length_3282_cov_4.146266, whole genome shotgun sequence.
ACCESSION   DAOPID010000176 DAOPID010000000
VERSION     DAOPID010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40429_length_3282_cov_4.146266"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="PK641_05940"
     CDS             complement(<1..426)
                     /locus_tag="PK641_05940"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPG55922.1"
                     /translation="MSKIAIAGLINIESTLKIDAFPIKYEPITYADFKIKTAVSGVAY
                     NITKALHTLGDEVNLISMTGDDKEAKQIETELLNEGLSMQYILPALKETPQSVILYDS
                     EGKRRIYCDLKDIQTCSYNTNIANDIFETCDAAVLCNINF"
     gene            complement(577..1272)
                     /locus_tag="PK641_05945"
     CDS             complement(577..1272)
                     /locus_tag="PK641_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55923.1"
                     /translation="MPSTNITMLQTVANGLEELKDDMVFVGGSVAELYVDNPAASDIR
                     PTLDVDCVIELSSRTAHAKLEEALRAKRFANDTSQGAPICRWIYQDIKVDIMPTDSNV
                     LGFTNIWYDEGVDNKIIKTLPDGTEIFVFPPEYYLAAKFEAHRGRGGNDLRQSHDFED
                     IIYIFDNCPEIVENIRNANQTVKNYLKQECRNLLGNDNLTEGIETALPYGSDEENTDI
                     ILELINRIAEIEL"
     gene            complement(1265..1768)
                     /locus_tag="PK641_05950"
     CDS             complement(1265..1768)
                     /locus_tag="PK641_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55924.1"
                     /translation="MRPQDIVVLIKIISIQQPNWRNIDIANSILISASEVSEALNRCK
                     IAGLIDSKKKKVNINSFLEFLIYGLKYVFPVQPGSIVKGIPTAHSASPIKEHISTGND
                     IYVWPYIKGGSRGQAIEPLYKNLPKAVENDVLFYELLAIIDTIRVGRVREIEIAIEEL
                     KKRLKNA"
     gene            complement(1839..2501)
                     /locus_tag="PK641_05955"
     CDS             complement(1839..2501)
                     /locus_tag="PK641_05955"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPG55925.1"
                     /translation="MSKRIFAIGDIHGCFDSLIELIENVISLKKEDKLILLGDYIDRG
                     LDSKKVLDYIITLQSNNYDIVTLHGNHESMLIDAYYNEDVTSKWIQNGGSETLKSFEV
                     DSLKAIDPKYINFCNNLKSHYELDEYIFVHAGLNDNLEDPFSDTYSMLWSSKEQYLNP
                     LLVDKIIIHAHSPVSLNFTQKQISNNSRVINIDGGRVYNNANWGKLIAIEVNTRTILF
                     VS"
     gene            2764..>3282
                     /gene="dapF"
                     /locus_tag="PK641_05960"
     CDS             2764..>3282
                     /gene="dapF"
                     /locus_tag="PK641_05960"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HPG55926.1"
                     /translation="MRFTKMHGIGNDYIYIDATKNVITNPELLAKKLSDRHYGIGSDG
                     LVLIMPSVICDFRMRMFNPDGTEAEMCGNASRCVAKYVYDNKMTDKTNFTLETGAGIK
                     ILSIHKNAENIVDTVTIDMGTPILEPEKIPVLASEEMVLNKSLVVLDKKYKINCISMG
                     NPHCVIFTEGIGL"
BASE COUNT         1086 a          553 c          473 g         1170 t
ORIGIN      
        1 aaaatttata ttacatagca cagccgcatc acatgtttca aaaatatcat ttgcaatatt
       61 tgtgttgtaa gagcaagtct gaatatcttt caaatcgcag tagatgcgtc ttttgccctc
      121 agaatcatac agtataaccg attgtggagt ctcttttagt gcaggtaaaa tgtattgcat
      181 acttaaaccc tcatttagca actctgtttc tatttgcttt gcctctttat catcccctgt
      241 catcgatatc aaattaactt catcgcccag tgtgtgtaag gcttttgtaa tattataggc
      301 tacgcctgat actgctgttt ttattttaaa atccgcataa gtaataggct catatttaat
      361 aggaaatgca tcaattttaa gtgtgctttc aatgttgata agtcctgcaa ttgcgatttt
      421 actcataatc gtatttctag attttataag aatctgatta gatgaatata attgaacaag
      481 tgtattttac agaaacttac taagtttgaa aaagcctaag acttctttag cgttttttgg
      541 acaacattga taatttatat gttgtttcct tattctctat agttctattt ccgcaatacg
      601 attgattaat tccaaaatta tatctgtatt ttcttcatcc gacccatatg gtaaagctgt
      661 ttcaatacct tccgttaaat tgtcattccc caacaagttc cggcattctt gttttagata
      721 attttttaca gtttgatttg cattcctaat attttctact atctctggac aattgtcaaa
      781 aatgtaaata atatcttcaa aatcatgact ttgtctgaga tcatttcctc ctcttcctct
      841 atgagcctcg aatttggctg caagataata ttccggtggg aatacaaata tttcggtacc
      901 atcaggtaaa gtttttataa tcttattgtc aactccttcg tcataccaga tattggtaaa
      961 tccaagtaca ttggagtcgg tgggcataat atcaacttta atatcctgat atatccatct
     1021 acaaattgga gctccttgag aggtgtcatt cgcaaatcgt tttgcccgta atgcttcttc
     1081 taattttgca tgagcagttc tcgaacttaa ttctataaca caatctacat ctaatgtagg
     1141 cctaatatca gatgcagccg gattatctac ataaagttct gcgacagaac ctccaacgaa
     1201 aaccatatca tcttttaact cctctaaccc attagcaaca gtttggagca ttgttatatt
     1261 tgtactaggc attctttagt cgttttttta attcttcaat agcaatctct atttcccgta
     1321 cacgaccaac tcgtattgta tcaataattg ctaataattc atagaataaa acatcatttt
     1381 caactgcttt aggtagattt ttatacagtg gttcaattgc ttgtccacga gaccctcctt
     1441 taatgtatgg ccacacataa atatcgttac ctgtagatat atgttcttta attggagaag
     1501 cagaatgagc agttgggatt ccttttacta tagaaccagg ttgaacagga aaaacatatt
     1561 ttaatccata gattaagaac tctagaaagg agttaatgtt tactttcttt tttttagaat
     1621 caataagtcc agcaatttta catctattta gtgcttcaga tacttcagaa gcactaataa
     1681 gaatagaatt agcgatatct atatttcgcc agttcggttg ctgtatagat attatcttta
     1741 tcaataccac tatgtcctgt ggtcgcattc cattgtgttt cttcatataa ataatgtata
     1801 attcgcgatt cgcgaattgc aaatatataa ctattatctt aactaacaaa tagaatagtt
     1861 ctagtattta cttcaatagc aataagttta ccccaattag cgttattata tactctacct
     1921 ccatcaatat tgatgactct actattattt gaaatttgtt tctgagtaaa attaagagat
     1981 accggagaat gagcatgaat gattatttta tctactaata aaggatttaa atattgctcc
     2041 ttactactcc atagcatact ataagtgtca gaaaaaggat cttcaagatt atcgtttaag
     2101 cctgcgtgaa caaagatgta ctcatctaat tcataatgcg atttcaaatt attgcagaag
     2161 tttatgtatt tggggtcaat agcttttaat gaatcgactt caaaactttt caatgtttca
     2221 gaaccgccat tttgtatcca tttagatgtt acatcttcat tgtaatatgc atcaatcaac
     2281 attgattcat gattaccatg caatgttact atatcataat tattactctg taaagtaata
     2341 atataatcaa ggactttttt actatctaat cctctatcaa tataatcacc tagaagaatt
     2401 agtttatctt ctttcttgag agatattaca ttttctatca attctataag agagtcaaaa
     2461 catccgtgaa tatcacctat ggcaaagatt cttttactca taatcgtatt tgttttgctc
     2521 tattctgtaa agataataca tttttttata atgcttatac tattaacgca agtatcgaat
     2581 tataataaaa ccagttgatt ttattataat ctataactat taaagcttta aatattagat
     2641 attatcacaa tgcaaattaa ttgtgtctaa aataactaaa aaataagttt ttcgaataca
     2701 aatgatagta tttttgcatt attaaaaaca ttatgatatg ttttaattaa aaataatgac
     2761 aaaatgagat ttaccaaaat gcatggtata ggaaatgatt acatttatat tgatgcaaca
     2821 aaaaatgtaa ttacgaatcc ggaacttctg gcaaaaaaat taagcgatcg ccactatgga
     2881 atcggttctg atggtttggt acttattatg ccttctgtca tttgtgattt tagaatgaga
     2941 atgtttaatc ctgacggaac agaagcagaa atgtgtggaa atgcttcacg ttgtgtagca
     3001 aaatatgttt acgacaacaa aatgaccgac aaaacaaatt ttacactcga aacaggagca
     3061 ggaataaaaa tcctaagcat acacaaaaat gccgaaaata tagtcgatac agtaacaatt
     3121 gacatgggaa caccaatatt agaacctgaa aaaatacctg ttttggcatc agaagagatg
     3181 gttttaaata agtccttagt tgttctcgat aaaaaatata aaataaactg tatttcaatg
     3241 ggtaatcctc actgcgtaat ctttacagaa ggcataggat ta
//
LOCUS       DAOPID010000177         3264 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_40742_length_3264_cov_3.302275, whole genome shotgun sequence.
ACCESSION   DAOPID010000177 DAOPID010000000
VERSION     DAOPID010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40742_length_3264_cov_3.302275"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1677)
                     /gene="gldF"
                     /locus_tag="PK641_05965"
     CDS             complement(<1..1677)
                     /gene="gldF"
                     /locus_tag="PK641_05965"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HPG55927.1"
                     /translation="MLVLLRKEIRTFFSSATGPIIIGVFLLLTGSFIWLLPGAYNVLD
                     SGYASLDGLFLIAPWVYLFLIPALTMRMFSEEYRTGTMELLLTKPVSRWSVLMSKFMA
                     GWILVVAALIPTLIYLYSVCLLGVPAGNIDFGGFWGSFLGLILLASVYVAIGVCASSL
                     TDNPVVSFILAAFVSFLLYAGFDSFSELFTDGRIQEIVSSLGIASHYDSMSRGVIDTR
                     DLVYFLTVSVFFIVITWWRMERKAWKHILEMVLIVVAINVVSSFFFTRFDLTTEKRYT
                     LSDNTKELMGSLQEELKITIYLDGDMNKGFLRLKKSVRDILEELNVYAVKGINYKFIN
                     PSDAANDKERNMRYAQLEARGMKPTVVYDKDNEGRMLQKVIFPWAECVSGKDTVRVNL
                     LKNISGKSGEENLNVSIETLEYEFTDAIRVFSNKNPDRIAFIEGHGEWDEAYVYDITK
                     SLSKYYYVDRGMLGSNISDLEPYKVIIIASPIAKYTESEKFIIDQYIMNGGRVLWLVD
                     GVRTDKENIGIANDVNLDDQLFTYGVRIDPVLLLDQQCALVPMNVSPEGQE"
     gene            1726..2973
                     /locus_tag="PK641_05970"
     CDS             1726..2973
                     /locus_tag="PK641_05970"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HPG55928.1"
                     /translation="MRKILFLSICLFTFGLIHANDFAPYKVKAQFIGGYIYPHDKNII
                     SPLVKGPALGGEIAFEWSLDGSKPWHLHYNKPDIGVALQVLDLSNPQIAGQMIATYTY
                     INIPMVKTNIVSFNTKIGGGLGFCTKPTDLAAAIADTRAIKDKAGDYNFAIGSPVNIN
                     INAGLSLDIKVHTNISLSAEILYNHFSNGSLAQPNGGLNLFDGHIGLKYMPVYKEAPQ
                     QAVVTKPAKRWYGEFIVSGGAKKLYYKDTQYFGCASINLGGYYRTCNQHRIGIGVDLF
                     YDGAYVPTARFDPNTSTYVDDNTYTSFGRTFITEDKIENKLRCGINIANDLIIGKFLI
                     GFQAGIYLYDPIKNCEPYDDAKEGKVNKGLIYGYNIQKEDGWNYFRISAKYYITKHLL
                     ANISIKTHLQKVEFVEFGIGVGF"
     gene            2986..>3264
                     /locus_tag="PK641_05975"
     CDS             2986..>3264
                     /locus_tag="PK641_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55929.1"
                     /translation="MKKVIIIIAALVALGFSIWGYYRMKSASVSQNTDIFISLPENAF
                     SILKINNLQNFSDALLYNNNYWRDLAKINDIGDIHYILSTLDSLKESSP"
BASE COUNT         1187 a          620 c          503 g          954 t
ORIGIN      
        1 ttcctgccct tccggagata cattcattgg cacaagtgca cattgttgat ctaataacaa
       61 tacagggtca atcctcacac cataagtaaa taattgatca tcaagattta cgtcattagc
      121 aataccaata ttctctttgt cagttcgtac tccatcaact aaccataaaa ccctgccacc
      181 attcattata tattggtcaa taataaattt ttcagattcg gtatattttg caatcggtga
      241 tgcaattatt ataactttat aaggttcaag atctgatata ttagaaccaa gcattcctct
      301 atctacgtaa tagtatttag acaatgattt tgttatatca tatacataag cttcatccca
      361 ttctccgtgt ccttctataa aagcaattct atcaggattc ttattcgaaa agactctaat
      421 tgcatcagta aattcatatt ctaatgtttc gatagataca ttcaagtttt cttcacctga
      481 cttaccggat atattcttta acaaattaac tcttactgta tccttaccac tcacacactc
      541 agcccacgga aaaataactt tttgcaacat gcgtccttca ttatccttat cataaacaac
      601 agtcggtttc ataccgcgag cttctaattg agcataacgc atatttcttt ctttgtcatt
      661 tgctgcatca gaaggattga taaatttata attaatacct tttactgcat atacatttaa
      721 ttcttccaaa atatcacgaa ctgatttctt taaacgcaag aaacctttat tcatatctcc
      781 atctaaataa atagtaattt ttaattcttc ttgcaaagaa cccattaact cctttgtatt
      841 atcagataga gtatagcgtt tttcggtagt aaggtcaaaa cgagtaaaaa agaaagacga
      901 aacgacatta atagcaacaa caatcaaaac catttcaaga atatgtttcc atgccttgcg
      961 ctccatcctc caccaagtaa ttacaataaa gaatacagat acggttagga aatatactaa
     1021 atcgcgagta tctataacac ccctactcat cgaatcataa tgcgaagcaa tacctaaact
     1081 cgatacaatt tcttgtatac gtccatctgt aaacaattca gaaaaagaat caaatccggc
     1141 atacaataag aaactgacaa aagcagctaa tataaatgat acaacaggat tatcagtcaa
     1201 tgaagatgca caaacaccaa tagcgacata gactgatgct aacaatataa gccctaagaa
     1261 cgagccccaa aatcctccga aatcaatatt tccggcaggc acacctaaaa gacatacact
     1321 atataaatat attaaggtgg gtattaatgc agctactacc aatatccaac cggccataaa
     1381 ttttgacatt aacacagacc aacgtgatac cggttttgta agcaaaagtt ccatggttcc
     1441 tgttctgtac tcttccgaaa acatgcgcat ggtcaatgcc gggataagga acaaatatac
     1501 ccacggagct attaaaaata aaccatcgag tgatgcataa ccggagtcta agacattata
     1561 tgcacccggc aaaagccaaa taaaagaacc tgtcagcaac aaaaacacac caataataat
     1621 tggtcctgtg gctgatgaaa aaaaagtacg aatctctttt cttaacaata caagcataat
     1681 aaagattttt gctaaatttg caaattgcaa agatagttta tttaaatgag gaaaatactt
     1741 tttttatcaa tatgtttatt tacgtttgga ttgatccatg cgaatgattt tgcaccatac
     1801 aaagtcaaag cacaattcat aggcggatac atatacccac acgataagaa tataatttca
     1861 ccattggtaa aaggtccggc acttggtggc gaaattgcat ttgaatggag tctggatggt
     1921 tcaaagccat ggcacttaca ctataataag cccgacatag gggttgcact acaagtactt
     1981 gatttgagta atccgcaaat cgcaggacaa atgattgcaa cctacaccta tataaatata
     2041 ccaatggtaa aaaccaatat agtatctttc aatacaaaaa taggtggagg acttggtttt
     2101 tgtaccaaac cgactgattt agctgcagca attgccgaca ctcgtgctat aaaagataaa
     2161 gcaggtgatt acaatttcgc aataggaagt ccggtaaata taaacattaa tgccggtttg
     2221 tctttagaca taaaagtaca tacaaacata agtttaagtg ctgaaatatt gtacaatcat
     2281 ttttcaaatg gcagccttgc gcaaccaaat ggcggattaa atttatttga cgggcatata
     2341 ggactaaaat atatgcctgt ttataaggaa gcaccacaac aagctgttgt aacaaaaccg
     2401 gccaaacgtt ggtacgggga atttatagta tcaggcgggg caaaaaaact atactataag
     2461 gatactcaat atttcggatg cgcaagcatc aatttaggag gatactatcg cacatgtaat
     2521 caacatcgca taggtatagg agtcgattta ttttatgatg gagcatacgt tcctaccgcc
     2581 agatttgacc caaataccag tacttatgta gatgataata catatacttc ttttggcagg
     2641 acattcatca cagaagataa gatagaaaat aaattaagat gtggaattaa tatagctaac
     2701 gatttgatta taggtaaatt cctaatagga tttcaggcag gtatatatct atacgatcca
     2761 ataaaaaatt gcgaaccata cgatgatgca aaagaaggta aagtaaataa aggacttatt
     2821 tacggctaca acatacagaa agaagacgga tggaattatt tccgaatttc tgctaaatat
     2881 tacattacaa aacatttact tgcaaatatc agtattaaaa cacatcttca aaaagttgag
     2941 ttcgttgaat ttggaatagg tgtaggattt taataatttc atagaatgaa aaaagtcatt
     3001 atcataatag cagcacttgt tgcactaggt ttttcaatat gggggtacta tcgcatgaaa
     3061 tcggcttctg tatcacaaaa tacagatatc ttcatttctt tgcctgaaaa cgcattcagc
     3121 atattaaaaa tcaacaactt acaaaacttt tcagacgccc tgttatacaa taataactat
     3181 tggagagatt tagctaaaat aaacgatatc ggtgatattc actacatact tagcacctta
     3241 gactcactta aagaaagttc tcct
//
LOCUS       DAOPID010000178         3239 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_41150_length_3239_cov_4.974874, whole genome shotgun sequence.
ACCESSION   DAOPID010000178 DAOPID010000000
VERSION     DAOPID010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41150_length_3239_cov_4.974874"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="PK641_05980"
     CDS             complement(<1..1052)
                     /locus_tag="PK641_05980"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPG55930.1"
                     /translation="MKYDIVIIGSGLGGLECGYILSKKGLKVCVLERANLLGGCLQSF
                     HRGNCLFDTGFHYVGGLDEGQSLNRLFKYFNLMNLDWVKMDNNFDDVCINGKTYPFVS
                     GHDNFRETLSSYFPHQKENLKAYTNFLQNVGIHTFDSFKPRSENDFYSSSLFAQSAHE
                     FLNNTISDPVLKDVLAGTSLKMELSEQLPLYVFAQINDSYIQGAWRLQGGGFQIAESL
                     ANSIIEMGGEVRKNSAVTRIIETNGKITSVEINNNESVDCSWVISNAHPIHTLSLIPE
                     ESIIRKLYRNRINRMENTFGVFTANIKLKSGSVKYKNKNLYAYHNADLWKYTEGKTDR
                     ALVSFYCPKNDDSCDC"
     gene            complement(1049..>3239)
                     /locus_tag="PK641_05985"
     CDS             complement(1049..>3239)
                     /locus_tag="PK641_05985"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPG55931.1"
                     /translation="SLLAKDKDSIINDITYNIPEEFLIDGIAYLYDNLPLYLDSTDYT
                     AMDTIFTKSHIERQMSDNYEELLSASGMYSYDIIRQDPAGLRYALKSKGKNISESMGG
                     SYKIINKHFFTPDSTVAVAFITPNFKAFDSKTGIRLTENIEAEIKAMNKIHPEIEILF
                     HGAPIQSVFNSRQIKTDLMMTMGLSLLFVCIIIAICFRNKSTIPFLLSPVIYGTFFAL
                     ACMYLTQGTMSLMAVGIGAIVLGVALSYCLHVLTHYKYLSDPEQVLRDQTRPVILGSL
                     TTIGAFMGLLFTESALLRDFGLFASLAMVGTTIFCLIFLPQFLNPEKNRKSEKVFRWL
                     EHINAYPLDKQKWLIITLVIISVICTIYSQWVCFDTNLKNIGYTNPNVARSSQILSDK
                     TAHGKLSTYFAVTSDNLDSALVYNERLGMRLDTMKQNGDISEFAHTSNILMPKYRQEA
                     NIKLWNSYWTADKKLEVKRILIECGAPYGFNAEVFEPFFTMIDRIYYPSSICESNVLP
                     KGLMSNWVEYTDSTYMLFTPVQMPKNNYRSTCDAIVAKPHTVVIDPFYYTQDMVRILN
                     NDFNIVLAISSLFVFLVLLISYRSLSLAIIAFIPMGLSWYIVLGIMSILGIQFNLINI
                     IVSSFIFGVGVDYSIFVMDGLLAGTRNYNKLLMFHKTAIFLSAIVLIISISSLIFATH
                     PAISSIGLTTLIGMTTTLLITYSLEPFLFHWLQKKSFFNRILQKRKA"
BASE COUNT         1061 a          568 c          515 g         1095 t
ORIGIN      
        1 aaacaatcgc acgaatcgtc atttttcggg caataaaaac taactaaagc tctatcagtt
       61 tttccttcag tatatttcca caagtcagcg ttatggtaag cgtaaagatt tttattttta
      121 tacttaacgc tgccactttt taattttata ttagcagtaa aaacaccgaa agtattttcc
      181 attctattga ttcggttcct atataacttg cgtattatac tttcttcagg tatcaacgat
      241 agggtatgaa tagggtgcgc atttgaaata acccaactac aatcaacaga ttcattatta
      301 ttgatttcga ctgatgttat tttgccatta gtttcaataa tacgtgtaac agcagaattt
      361 tttctaactt cgccacccat ttcgattatt gagttggcta aagattctgc aatttggaat
      421 ccaccgcctt gcagacgcca tgcaccttgt atataagaat catttatctg tgcaaaaaca
      481 tatagcggca attgctccga taattccatt tttaaagaag ttccggcaag aacatctttt
      541 aaaacaggat cacttattgt gttgtttaaa aattcgtgtg ccgattgcgc aaataatgaa
      601 gaactataaa agtcattttc agaacgaggt ttgaagctat cgaatgtatg aatcccgaca
      661 ttttgtaaga aattagtata cgctttcaaa ttttctttct gatgaggaaa atacgagctt
      721 aatgtttctc taaaattatc atgtccggat acaaaaggat atgttttacc atttatgcat
      781 acatcatcga aattgttatc catttttacc caatccaggt tcatcagatt aaaatactta
      841 aacaatctgt ttagtgattg tccttcatct aatccgccta cataatggaa acctgtgtca
      901 aatagacaat tacctctgtg aaaggattgc aagcaccctc caagtaaatt tgctctttcc
      961 agaacacata ctttcaaacc ttttttcgaa agtatgtaac cgcattcaag accgccaagc
     1021 ccacttccta tgataacaat gtcgtacttc acgcttttct tttttgtaat atgcggttaa
     1081 aaaaagattt cttttgcaac caatggaata agaatggttc taatgaatat gtaataagta
     1141 atgttgtagt cattcctatt aatgtagtta gtccgattga tgaaattgca gggtgagtag
     1201 caaaaatcaa ggacgaaata cttattatta gaacaattgc agacaagaat atcgctgttt
     1261 tatggaacat cagtaactta ttataattac gtgttccggc cagcaatccg tccataacga
     1321 atatactata atctacacct actccaaata tgaatgatga aacaataata ttgattaggt
     1381 taaactgtat accgagaata ctcataatac ccaacacaat gtaccaactt aatcccatcg
     1441 gtataaatgc aattattgct aaagacaaac ttctgtatga aatcaataat acaagaaata
     1501 caaataacga tgatatagct aaaactatat tgaagtcatt atttagtatg cggaccatat
     1561 cttgtgtata gtagaatggg tcaattacta cagtatgtgg ctttgcaact atagcatcac
     1621 atgtacttct atagttattt ttaggcatct gtacaggtgt aaataacatg tatgttgaat
     1681 cagtatattc tacccagtta gacattaaac ctttgggtaa aacattagat tcgcatattg
     1741 aagatggata atatattctg tctatcatag taaagaatgg ctcaaatact tctgcattaa
     1801 atccgtaagg agcaccgcat tctatcaata ttcttttaac ttctaatttt ttatctgcag
     1861 tccaatatga gttccacaat tttatgttgg cctcttgtct gtattttggc atcaatatgt
     1921 ttgacgtatg agcaaattca gaaatatcac cattctgttt catagtgtct aaacgcatcc
     1981 ctaaacgttc gttgtataca agtgcagaat ctaaattatc agaagttaca gcaaagtaag
     2041 ttgacaattt tccgtgtgca gttttgtctg atagtatttg agatgaacgt gcaacatttg
     2101 gattagtata accgatattt tttaaattag tatcgaagca tacccattgt gaatatatag
     2161 tacaaattac acttataatt actaatgtaa taattaacca tttttgcttg tctaatgggt
     2221 atgcattgat gtgctctaac caacggaata ccttttcaga tttacgattt ttctcaggat
     2281 ttaagaattg aggtaagaaa ataagacaga atattgttgt accaaccatt gccaaagatg
     2341 caaacaaacc gaaatcacgc agtaatgcag actctgtaaa aagaagtccc ataaaagctc
     2401 cgattgtggt taaactacct aaaattacag gtcttgtctg atctcttaat acttgttcag
     2461 gatcactcaa gtatttgtag tgtgttagta cgtgtaaaca ataacttaat gctacgccaa
     2521 gtacaattgc acctattccg actgccataa gtgacattgt accttgtgtt aaatacatgc
     2581 aagccaatgc aaagaaagta ccatatatta ccggagataa tagaaatgga attgttgatt
     2641 tatttcgaaa acatatagca attattatgc atacaaatag taatgacaat cccattgtca
     2701 tcatcaaatc ggtttttatt tgtcgcgagt taaaaacact ttgaattggg gccccatgaa
     2761 acaatatttc tatttcagga tgaattttat tcattgcttt tatttcggct tcaatatttt
     2821 ctgtcaagcg tatacctgtc tttgaatcaa aggctttaaa attaggagtg ataaatgcta
     2881 ctgcaacagt tgagtcgggt gtgaagaaat gtttgtttat gattttgtaa cttccaccca
     2941 tgctttcgct aatatttttc cccttgcttt ttaaagcata tcttaaacct gcagggtctt
     3001 gtctgataat atcatatgag tacattccac tggccgaaag caattcctca taattgtctg
     3061 acatttgtct ttcaatgtgc gattttgtga atattgtatc catcgctgta taatctgttg
     3121 aatccagata caaaggcagg ttatcatata aatatgcgat tccgtcaatt aaaaattctt
     3181 ccggaatatt atatgtgatg tcgttgataa tagaatcctt gtcttttgcc aacagcgaa
//
LOCUS       DAOPID010000179         3206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_41754_length_3206_cov_4.608061, whole genome shotgun sequence.
ACCESSION   DAOPID010000179 DAOPID010000000
VERSION     DAOPID010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41754_length_3206_cov_4.608061"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PK641_05990"
     CDS             complement(<1..390)
                     /locus_tag="PK641_05990"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPG55932.1"
                     /translation="METIQSILSSDTFPWLSAILLGLLTAINPCQLAMNLMAIGYISK
                     ETPKHAFKYSLIYALGRIFVYTILGIALIYILHQGAAILPIQEFFGQYGELLLSPVLI
                     VVGSVILIHGLGHDHSLTDGDNCHHHTK"
     gene            613..969
                     /locus_tag="PK641_05995"
     CDS             613..969
                     /locus_tag="PK641_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55933.1"
                     /translation="MKKIYSFLFVALISLCLASCNKVEPSSLVGAVYETPEDYDMKLT
                     FNTTTTCILSVGYGDGRYDENYATYSVQGDKITLVPSGVVSEQMEFLIKENGNLLMLT
                     MGEGEGSIGDIPFYKK"
     gene            1288..2313
                     /locus_tag="PK641_06000"
     CDS             1288..2313
                     /locus_tag="PK641_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HPG55934.1"
                     /translation="MKEIDKKLIDAVKQYRSSGIEQQVDYERFYLYSLITHSTAIEGS
                     TITELENQLLFDEGIAAKGRSIDEQMMNLDLKNAYLFGFEWAKTFAPYTVDFLCQLSA
                     KVMRRTGAGYSTMGGNFDSSKGELRLCNVSAGIGGKSYLSFQKVPGATIDFCNWLNNE
                     ISSVNKTDIVACYRLSFEAHYRLVSIHPWVDGNGRTTRLLMNIVQQQLGLVPSIVAKE
                     TKEAYIQALIDSRESKDSTIIQDLMIAQHIANINRRIIQYIGNAIDDTIDDTVNDTVN
                     DTVNSSDMDSQLLTLIKEHPEYTYEEYAQKLNVGRATIARHIKKLSGTMIERIGSDKN
                     GYWVLIK"
     gene            complement(2404..>3206)
                     /gene="ilvC"
                     /locus_tag="PK641_06005"
     CDS             complement(2404..>3206)
                     /gene="ilvC"
                     /locus_tag="PK641_06005"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HPG55935.1"
                     /translation="DIEEACQRGTIIQYLLSDAAQIELWPVVKRNLTAGKTLYFSHGF
                     GITWNDRTGIVPPKDVDVIMVAPKGSGTSLRRMFLQGRGLNSSYAIWQDATGHAFDTV
                     VALGVGVGSGYLFETTFLREATSDLTGERGSLMGAIQGLLLAQYETLRENGHEPSEAF
                     NETVEELTQSLMPLFAENGMDWMYANCSTTAQRGALDWMGPFHDAIKPVMQKLYASVK
                     SGNEAQISIDSNSKPDYRAGLEKELKALRESEMWQAGAVVRKLRPENN"
BASE COUNT         1048 a          513 c          629 g         1016 t
ORIGIN      
        1 ctttgtgtga tggtggcagt tgtctccatc tgtaagacta tgatcgtgac ctaggccatg
       61 tattaaaatc acagaaccta caacaataag caccgggctc aataacaatt ctccatattg
      121 accaaagaac tcttgtatag ggagaattgc tgctccttgg tgtaatatgt aaatcagcgc
      181 gattccaagt atggtatata caaatattcg accaagtgca tatatcaaac tgtatttaaa
      241 agcatgtttt ggtgtctctt tggaaatata tcctattgcc atcagattca ttgccagctg
      301 acatggatta atagctgtta gtagtcccaa aagtatggca ctaagccatg gaaaagtatc
      361 agaacttaaa atcgattgta tggtttccat aaatgcatcc ggttgatttt actgcaaaga
      421 tactaagaag ttggtgatta aggcttatgc gcattgcgct ttgattgtgg tggtaaaaat
      481 tattaaataa gttagaaata tttatttaac taattttaac tattgggggg gtaaaattga
      541 ttttttttac cacctttgtg gtgaaaatat aggtactcat aaatttatta attattgttt
      601 taatttatat atatgaaaaa aatttattcg tttttgtttg tagcattaat ttcgctttgc
      661 ttagcatcgt gtaataaagt agaaccaagt tctttggttg gcgcagttta tgaaactccg
      721 gaagactatg acatgaaact aacgtttaat acaactacaa catgtattct tagtgttggt
      781 tatggcgatg ggagatatga cgaaaattat gctacttatt cagtgcaagg tgataaaatt
      841 acactggttc cttctggtgt agtaagcgaa caaatggagt tccttattaa agagaatggt
      901 aatctattaa tgttaactat gggagaagga gaaggtagta ttggagatat acctttctac
      961 aaaaaataat ctcaattaaa tagttttgat taaaaagggg ctgagtttca gtcccttttt
     1021 tttgttgtaa atattgaaac tcaaaagtag acataagaaa aatagcattt ttggtaatgc
     1081 aaaaattgcg taattaaatc aggagattat tgtaaaatgc cattgtaaga ttgcgggatg
     1141 ggaaatactt aatatatttg cattaagttc agagttgtat tgaaaagtag tcaactattt
     1201 ttttgaaaga aaacataagc aacaccttat attgagttaa taaaagatag cttgagataa
     1261 ctgatatttt tcataaaaca gactattatg aaagagatag acaaaaaact aatagatgcg
     1321 gtcaaacaat atcgttcttc gggtatagag caacaggtag attatgagag attctacttg
     1381 tattcgctta ttacccattc tacggctatt gagggatcta ctattacaga acttgaaaat
     1441 caattgctat ttgacgaggg cattgctgct aaaggaagaa gcattgacga gcagatgatg
     1501 aatcttgacc ttaaaaacgc ttatcttttt ggctttgagt gggcaaagac tttcgcaccc
     1561 tatacggtag atttcttgtg tcaattgtct gctaaggtga tgagaagaac aggagcaggg
     1621 tattctacaa tgggaggaaa ttttgattcg tcaaaaggag aattgcgtct ttgcaatgtg
     1681 agtgccggaa taggtggtaa aagttatcta tcatttcaaa aagtgcctgg ggcaactatc
     1741 gatttctgta attggcttaa taatgaaatc tcatctgtaa ataaaacgga tatagttgct
     1801 tgttatcgat tgagttttga agcgcactat cgtcttgtta gcattcatcc ttgggttgat
     1861 ggcaatggtc gtactacaag attacttatg aatattgtgc aacagcaatt aggtttagtc
     1921 ccgtctattg ttgcaaaaga gacaaaagaa gcctatattc aagctttgat tgacagcaga
     1981 gagagtaaag atagtactat tattcaagac ttaatgattg ctcagcatat tgctaatatt
     2041 aacaggagaa tcatacaata tataggtaat gctatagatg atactataga tgatactgta
     2101 aatgatactg taaatgatac tgtaaatagc tcggatatgg attctcaact tttgacactg
     2161 ataaaagagc atcctgaata tacttatgaa gaatatgcac agaagttgaa cgtagggcgt
     2221 gccaccattg ccagacacat aaaaaaactt agcggcacta tgattgagcg catcggatct
     2281 gataaaaacg gatattgggt attaataaag tgataagcta attcgagtat ttcacctaca
     2341 taaaataata aaggagatgc aaattaaacc gcatctcctt tatttattgt gattaaatta
     2401 atattaattg ttttcaggac gcaatttacg tacaactgca ccggcttgcc acatttcgct
     2461 ttcgcgtagg gctttcaatt ctttttctaa accggcacga tagtctggtt ttgaattaga
     2521 atcaattgaa atttgagctt cgttaccgct ttttacgctg gcatataatt tttgcataac
     2581 aggtttaatt gcatcgtgga aaggtcccat ccagtccaat gcaccgcgtt gtgctgttgt
     2641 agagcagtta gcatacatcc aatccatacc gttttcagca aacaaaggca tcaatgactg
     2701 agttaactct tcaactgttt cgttgaatgc ttctgatggt tcgtgtccgt tttcacgcaa
     2761 tgtttcgtat tgtgctaaaa gcaaaccttg tatagcaccc atcaaagaac cgcgttcgcc
     2821 tgtaaggtct gatgtagctt cgcgtaagaa tgttgtttcg aacaaatagc cggaaccaac
     2881 acctacacct agagcaacaa cagtatcaaa agcatgacct gtagcatctt gccaaatagc
     2941 ataagatgag tttaaaccac ggccttgtaa gaacatacga cgaagtgatg ttcctgaacc
     3001 tttaggagca accatgataa catcaacgtc ttttggagga acaatgcctg tacgatcatt
     3061 ccatgtaatt ccaaatccgt gagagaaata taatgttttt cctgctgtaa gatttctttt
     3121 tacaaccggc cataattcga tttgagctgc atcagacaat aaatattgta tgatagtacc
     3181 acgttgacaa gcttcctcaa tatcaa
//
LOCUS       DAOPID010000180         3188 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_42080_length_3188_cov_2.585701, whole genome shotgun sequence.
ACCESSION   DAOPID010000180 DAOPID010000000
VERSION     DAOPID010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42080_length_3188_cov_2.585701"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3188)
                     /locus_tag="PK641_06010"
     CDS             complement(<1..>3188)
                     /locus_tag="PK641_06010"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55936.1"
                     /translation="NQCYGVPSGSIQLSVVGGSPAYTYTWSGPFVNPNQKDQFNIPGG
                     LYQVRVQDANQCEISEHYDLIKPTELDVSVSQTDVSCFGLSDGRLHASIVGGSKPYVV
                     KWENQSGTLVVNDTIAEGLPVGTYTLTIVDSLSCVLNRTYTITQPTALDAQMDITSVI
                     CTGATDGRASVSVTSGSGTAPYSYKWTKNPNTDLGSGYSKSNLGEGSYTVVVTDHNGC
                     MVSISNGITESIPIQIGVVDIENVQIIGAATGHIILNTTGGFGNLTYTWHGDGIQPGA
                     EHDRDQYQLKAGWYYLTVEDDLSCSETMQFEITEPSVLVVKADVHQITCFGLNNAYIE
                     MSVTGGTNTTYSWKYNGVDISTDKNIYDLSPGDYLLTLTDVSGIFTYNYLIIEPSELT
                     LNQLASSKLALNCAGDKDGVINIEVNGGTPQYTLSWTGPDIPHVVTNPYGVSDLGKGL
                     YRVDITDANKCYVSRSFQITEPDTLLLSANIGLNTCNGDANGTITLAVNGGTPAYIYK
                     WTGIGVDEYAQNQSSLIGGRYNLRITDQNLCEIDTAFVINNPASLQANITGISDICEG
                     DEVTLYMVSSGVAPWTFVYTDGLNDYTLVSDRPDTSVVVKPLIDTEYSLVSVEDGMGC
                     EAKLSGKVSVTVHKYPSLQVLSVSEDCCLGDEILVDLVISNEGPWTLKYSDDGGSNVI
                     YDITKMRDTLRIAPLRAGNIQYTIDSISTAYCTTKIDYSFDVIVYPYPNLIVTMPAFV
                     CQSDTLHIALEPQGVGPWQLVYYENGIRYFEDIDTSPYDLVRTPDKIDNNYLFESITT
                     GQYCTKNLNKDYTVSLESLPGDAMAIIGNNAACRGTLERYYLPEIINADKYEWILPKG
                     FTVMSGTNTNDITVSIGLDAEKGTIRVHGVNSCGNGGDATLAVDLDEPFGPVGDISAP
                     LYICENEALFQLSIDDVEGATLYEWAVPEGYSIISGEHSSSILVSIDSYAKSGTVSIT
                     PTNRCASADKKEKYLSIRTLPLAEAGVDLATNCINSVRLGATDVAGTVAKWTSLRGEV
                     VFSDPAAYNSEVSGLSYGRNPFRWDVNDGYCL"
BASE COUNT          986 a          664 c          495 g         1043 t
ORIGIN      
        1 tcaagcaata gccgtcatta acatcccatc taaaaggatt ccgtccataa gacaaacctg
       61 acacctctga attatatgct gcaggatctg agaagaccac ttcaccacga agtgaagtcc
      121 atttagcaac tgttcctgca acgtcggttg cacctaatct tacagaatta atacagtttg
      181 ttgctaaatc gacacctgct tctgctaatg gcaatgttct aatgcttagg tatttttctt
      241 ttttgtcggc actggcacaa cgattagttg gtgttataga aacggttcca ctcttagcgt
      301 acgaatcaat agaaaccaat atacttgatg aatgttctcc ggatattatt gaatagcctt
      361 caggaacagc ccattcatac aaagttgccc cttctacatc gtctattgat aattggaata
      421 atgcttcgtt ttcgcaaatg tatagaggtg cgcttatatc accaacaggt ccaaacggtt
      481 cgtctaaatc tactgccaat gttgcatcac caccatttcc gcaagaattt acaccatgta
      541 ctctgatagt tcctttctct gcatctaatc caattgatac ggtgatatca ttagtattag
      601 ttccactcat taccgtaaag ccttttggta atatccattc atatttatct gcattaataa
      661 tttcaggcag ataataacgt tctaacgtgc ctctacatgc tgcattattt cctatgattg
      721 ccattgcatc accgggtagg ctttctaatg aaactgtgta atctttattt aagttctttg
      781 tacaatattg acctgtggta atactttcga ataaataatt gttatctatt ttatcaggag
      841 tacgtaccaa atcgtatggg cttgtgtcaa tatcttcgaa ataacgtatt ccattttcgt
      901 aataaactaa ctgccaagga ccgactcctt gtggttctaa tgctatatgc aaagtatctg
      961 attgacatac aaaagcaggc attgttacaa tcaaattagg atacggatat actataacat
     1021 caaagctata atcaatcttt gttgtacaat atgcagtact gatactatcg atggtatatt
     1081 gaatattacc tgcacgcaat ggagcaatac gtaaggtatc tctcatttta gtaatatcat
     1141 atattacgtt agaaccacca tcatcactat atttaagagt ccaaggacct tcgtttgata
     1201 ttactaaatc tactaatatt tcgtcaccaa gacagcaatc ttcagataca gataaaacct
     1261 gcaaactagg atatttgtgt actgttactg aaaccttacc acttaattta gcttcacatc
     1321 ccataccatc ttctacagat acaagactat attctgtatc aatcaatggt ttaacaacaa
     1381 cactcgtatc aggacgatcg gaaactaagg tatagtcatt caaaccatct gtatatacaa
     1441 atgtccaagg agctactccc gaagatacca tgtataatgt aacttcatca ccttcacaaa
     1501 tatctgaaat gcctgtaata tttgcttgta aacttgccgg attattaatt acaaaagctg
     1561 tatctatttc acaaagattc tgatcagtta tgcgcaaatt ataacgacct cctataagtg
     1621 agctttggtt ttgcgcatat tcatcaactc ctattcctgt ccatttgtat atatatgcag
     1681 gagtgccgcc attaactgct aatgtaattg ttccatttgc atctccatta cacgtattca
     1741 aaccgatatt tgcagataat aacaatgtat ccggttctgt tatttggaaa cttctactaa
     1801 catagcattt attggcatca gtaatatcta ctcgatacaa gcccttgcct aaatcagaga
     1861 ctccataagg atttgttact acatgaggaa tgtcaggtcc tgtccatgat aatgtatatt
     1921 gtggagtgcc gccattaact tctatattaa taacaccatc tttatctccg gcacaattca
     1981 atgctagttt gcttgatgcc aattgattaa gtgttaattc agaaggctca ataattaaat
     2041 aattgtatgt aaatatgcca cttacgtctg tcaatgtaag tagatagtca cccggagaca
     2101 aatcgtatat attcttatca gttgaaatgt ccacaccatt atatttccaa ctatatgtag
     2161 tattagttcc acctgtaaca gacatttcta tgtaagcatt attcaatcca aaacatgtaa
     2221 tctgatgcac atctgctttt acaaccaata cgcttggttc ggtaatttca aactgcatag
     2281 tttcgctgca agacaaatca tcttcaacag ttaaataata ccaacctgct tttaattgat
     2341 actgatcacg atcgtgttct gctcctggct gtattccatc accatgccat gtataagtca
     2401 aattgccaaa gcctccagta gtattgagta ttatatgtcc tgttgctgca cctataattt
     2461 gtacattttc gatatctacc actccaattt gaatagggat tgattcggtt atgccattgg
     2521 atatcgatac catacaacca ttatggtctg taactacaac tgtatatgaa ccttcaccta
     2581 aatttgactt gctataacct gaaccaaggt cagtatttgg attttttgtc catttgtaac
     2641 tataaggagc tgtacctgat ccactggtta cagatactga tgcgcgacca tctgtagcac
     2701 ctgtacaaat aactgaggta atatccattt gagcatccaa tgctgttggc tgagttattg
     2761 tatatgttct attaagaaca catgacagac tatctactat agttaatgta tatgttccaa
     2821 caggaagacc ttctgcaatt gtatcgttaa caactaaagt acctgattga ttttcccact
     2881 taactacata tggtttactg cctccaacaa tagacgcatg taaacgtccg tctgaaagac
     2941 caaaacaact cacatctgtt tgcgaaacag aaacatctaa ttcagtaggt ttaattaaat
     3001 cataatgttc gcttatttca cattgatttg catcctgtac acgaacttga tataaacctc
     3061 cggggatatt aaattgatct ttttgatttg gattaacaaa tggccctgac caagtatatg
     3121 tataagcagg agatccgcca actacactta actgtattga acctgatggt actccataac
     3181 actgattc
//
LOCUS       DAOPID010000181         3181 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_42211_length_3181_cov_3.246641, whole genome shotgun sequence.
ACCESSION   DAOPID010000181 DAOPID010000000
VERSION     DAOPID010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42211_length_3181_cov_3.246641"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..1987)
                     /locus_tag="PK641_06015"
     CDS             complement(125..1987)
                     /locus_tag="PK641_06015"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPG55937.1"
                     /translation="MKTFEELGLQASILESISELGFEHPSPIQEEVIPYLLANTGDVV
                     GLAQTGTGKTAAFGLPMLQLVDSNSRSTQGLILAPTRELCVQIATEMGRYGAKLPNTR
                     IVAVYGGEDIRKQLKDLDRTPQILVATPGRLLDLIKRKKVFLDKISLLVLDEADEMLN
                     MGFLDDITEIIEQTSDDRHTLLFSATMPREIASIASRYMHNAKEISVGKRNEAAKNVE
                     HNYVLTKPTCRYEVLKRILDFNPDIYGIVFCRTRQETKDVADKLMIDGYNADALHGDL
                     SQAQRDSVMSKFRIRNLQILVATDVAARGLDVNDLTHVIHYNLPDDRDTYTHRSGRTG
                     RVNKSGISIALITPREKGAIRPIEKEINRTFIQMDIPSGLEVCQRQVLNMAERMQQVE
                     VDEKLQKYLPNILELLKDMSKEEVISRFISLEFNRFLEYYKNAPDLNSMMKREAVSGD
                     GDFKKSRRDVRRIDKGNTIRLKIDKGRRDGFEPKRLLGLINDVTNDKSINIGDIDVSP
                     KFTFFDVDKSQLSRLIDAFANNPKAKGVQINEVKGEQKSSSGRSGGDRGERRDDNRRS
                     SSGSRDSRSSRDSRDSRDGQSFGRRRERVEDKRPSEHSTSSSQKEDRISKRKRW"
     gene            2235..3181
                     /locus_tag="PK641_06020"
                     /pseudo
     CDS             2235..3181
                     /locus_tag="PK641_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844660.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
BASE COUNT          974 a          633 c          541 g         1033 t
ORIGIN      
        1 caaagaaaat gaactggggt ccggggcgaa gccacggtag gcacgaagtg catatgttta
       61 atttgaaggg ttatgggtga agggtgaagg gataaaaaaa gaggcttcag cctctttttt
      121 tatcttacca acgtttacgt ttacttatgc gatcttcttt ttgcgaagag cttgttgaat
      181 gttcacttgg tcgtttgtct tcgactcttt cgcgtcttct tccaaaagat tgtccgtctc
      241 tcgaatctct cgaatcccta gaacttcttg aatctctcga accgctgcta ctacgtctgt
      301 tatcatctct tctttcgcct ctgtcaccac cagatcttcc tgatgatgat ttttgctctc
      361 cttttacctc attaatttga acacctttag cttttggatt gtttgcaaag gcatcaatta
      421 aacgcgataa ctgactttta tcaacatcaa agaatgtaaa tttaggactt acatcaatat
      481 caccgatatt tatactctta tcgttagtta catcattgat taaaccgagt aaacgtttag
      541 gttcaaaacc atctcgtcta cctttgtcaa ttttcaaacg aatagtattg cctttgtcta
      601 ttctacgtac atctcgtctg ctctttttga aatctccatc acccgaaacg gcttcgcgtt
      661 tcatcataga gtttaagtca ggagcatttt tataatactc aaggaaacga ttaaattcca
      721 aagaaataaa tcggcttata acttcctctt tcgacatatc tttcagtaat tcaagtatgt
      781 taggaagata tttctgaagt ttttcgtcaa cttctacttg ttgcatacgt tcagccatat
      841 ttaatacttg acgttggcat acttcaagtc ccgatggaat atccatctgt ataaatgttc
      901 tgtttatttc cttctcaata gggcggattg cgcctttttc tctaggagtt atcaaagcaa
      961 tagaaatacc cgatttgttt acacgtcctg ttctaccact tctatgtgta taggtatcgc
     1021 ggtcgtcagg taaattgtaa tggattacgt gtgtaagatc gtttacatcc aaaccacgtg
     1081 cagcaacgtc agtagcaacc aatatttgca gattcctgat tctgaactta ctcataacag
     1141 aatcacgttg agcttgcgac aaatcaccat gcaaagcatc agcattatat ccgtcaatca
     1201 tcaatttatc cgcaacatcc ttagtttctt gtcttgtacg gcaaaatact ataccatata
     1261 tatcaggatt gaaatctaaa atgcgtttta aaacctcata acgacatgtt ggtttggtca
     1321 aaacgtaatt atgttctaca tttttagcag cctcattacg tttacctacc gaaatttctt
     1381 tagcattatg catataacga cttgcgattg atgcaatttc gcgtggcata gttgcagaaa
     1441 ataacaaagt atgacgatcg tctgatgtct gttcgataat ttctgtaata tcgtccaaaa
     1501 atcccatatt aagcatttca tcagcttcgt ccaaaaccag caacgatatc ttatctaaaa
     1561 ataccttttt acgtttaatt aaatcaagta gacgtccagg agtggcaact aaaatttgcg
     1621 gtgttctgtc taaatctttt aattgtttgc gaatatcctc acctccatat accgcaacaa
     1681 tacgtgtatt aggaagtttt gctccataac ggcccatttc tgtagctatc tgaacgcata
     1741 attcgcgagt aggagctaat atcaaaccct gagtactcct cgaatttgaa tcaactaatt
     1801 gcaacatagg caatccgaat gcagctgttt ttcctgttcc tgtttgtgca agtccaacaa
     1861 cgtcgcctgt gtttgccaat aaatacggaa taacttcttc ctgaatagga gaaggatgtt
     1921 caaacccgag ttccgaaata ctctcgagaa tggaggcctg taggcctaac tcttcaaatg
     1981 ttttcatgaa aatttttctg tgcagcaata gaaggtaggg actatgaacc ctgtcagccg
     2041 catctctttt taaatttatt taccccttag aagaatctgt ttatcttccc aagcgtataa
     2101 acaaattcca aaatcgctgc aaagatagta aaaataattg acatacaaaa gtttataaat
     2161 aaagcgtaca aataccgcta ttacttccaa ctaaatttta tatatttgtg gaatattttc
     2221 gaaaaaatga aaaaatgatc aacaagctac atagaaaaaa caattctcct tggattaagg
     2281 ttatttggat attatttgca tcagtggtag ttacagcagt acttatgttt actctaatct
     2341 cctatggggt aataggttac ataccggata tcgaagaact acagaaccct aaaaacaaat
     2401 ttgcaacaga gttatataca tcagacggcg ttcttttagg tcgttatttc tatggtaaag
     2461 acaatagagt tgcagttgct tatgccgaca tatctcctta tatggtacaa gctttaattt
     2521 ctactgaaga tgctcgtttt tacggccatt cgggtattga tgttaaatct ttgattcgtt
     2581 ctatagtttt tagaggctta ttaggtatta aaagtgcagg cggtggtagt acaatcactc
     2641 aacaattggc aaaattgtta tattcgccaa ctgccgaaag tatttttcaa agagccttac
     2701 aaaaacctat tgaatgggtt atagctgcga agctcgaaaa gtattatact aaagaagaaa
     2761 tcatagccat gtaccttaat cagttcgact ttttgaacaa tgctgtgggt attaaatcgg
     2821 catctcaaat ttattttaat acaacacccg ataaacttaa catagagcaa tctgcaatgt
     2881 tggtcggaat gtgtaaaaat ccatcatatt ttaatcctaa tcgctttccc gaacgcactc
     2941 aaggcagacg aaatacagtt ttgtcgcaga tggaaaagat ggattacatt actaccgagc
     3001 agtgtgattc gttacaactg atacctatta aattagattt taggaaagcc gatcacaaag
     3061 ccggtctagc tccatatttc agagaatatt tacgtcaaat tttaacggca cccgaaccta
     3121 aacgcaaaaa ttacgcatca tggcaataaa aaatattacg aagattcaat tgcctgggtt
     3181 a
//
LOCUS       DAOPID010000182         3155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_42665_length_3155_cov_3.642581, whole genome shotgun sequence.
ACCESSION   DAOPID010000182 DAOPID010000000
VERSION     DAOPID010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42665_length_3155_cov_3.642581"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PK641_06025"
     CDS             complement(<1..279)
                     /locus_tag="PK641_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55938.1"
                     /translation="MKKYAPHLVAVLVFLILTFMYLSPVFQGKTIFGQDTRSYVGMSK
                     EATDYNATHDEQTLWTNSMFGGMPTYQISMDQPSSILKSTASVDVRECS"
     gene            384..456
                     /locus_tag="PK641_06030"
     tRNA            384..456
                     /locus_tag="PK641_06030"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:417..419,aa:Pro,seq:cgg)
     gene            512..586
                     /locus_tag="PK641_06035"
     tRNA            512..586
                     /locus_tag="PK641_06035"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:547..549,aa:Pro,seq:ggg)
     gene            713..1108
                     /locus_tag="PK641_06040"
     CDS             713..1108
                     /locus_tag="PK641_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55939.1"
                     /translation="MKNSTPKYYRFYNTATLKYSSLSLLFCITLCFALLSSYSVEDKK
                     QDNIPQPNTGFIWETQNLPIQYLCTPTRQIIISDGRHSVNYGFSAFYLLKTNTLKIAQ
                     RISKMTKPVFLPELAELKTCLLYPFHGFW"
     assembly_gap    1508..1517
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1585..>3155)
                     /locus_tag="PK641_06045"
     CDS             complement(1585..>3155)
                     /locus_tag="PK641_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55940.1"
                     /translation="VSINANYTGVALVIDDKVYSTLTEAVNNASNKDVVLLNDVEENL
                     VIKTSLVLDGGNYSINNLIVNANGSLVLNGDITVASFNIQAQNGASGEFREDGGHLTV
                     LGSVSMDKSLDPSGTVSPKKWYAFSVPFPVSLENGIYRKSGDQIIKMVYGDQYYVQEY
                     DGAKRASTGSGWVMTDVKSTLQPGKFYIFASNGYNDVRFVKTEGSPLVSSQTTLSVSE
                     FASAKIKDTGWNGLANTQLFHVDVNSDVMFAQVYKNGAKEYLTVSLWDESFVVGVPFF
                     IQQGNGMSSVLDLTKRTRNSLRGASENINSIYNLRIATAGGEMSDQLFVSANESASES
                     YVIGKDVEKMGTPTSASTAQLWCVDYDNMNLSVYEAKLKDGAAHLAFRMYAPAAGNYS
                     LYLNTNKIPNDGSTLYLLKDGVAVHNFNNDGEYSFASSKGLISNYELLIKAADDITTV
                     VSENMGNGVFAYIEGNVLYIKNMSEFSSYAVGNSIRTLYSGESDGSNLALPVIEKGMY
                     WIKVGNQTIKLIKK"
BASE COUNT         1014 a          630 c          482 g         1019 t
ORIGIN      
        1 ggagcattcc ctcacgtcca cgctcgcagt gctcttcaaa atactcgaag gctgatccat
       61 tgatatttga tatgtaggca taccaccaaa catcgaatta gtccacaaag tttgttcgtc
      121 gtgagttgcg ttgtagtcag tagcttcctt actcattcct acataacttc ttgtatcttg
      181 tccgaatatc gttttaccct gaaatacagg agacaaatac ataaatgtca gaattaagaa
      241 aactaaaact gccacaaggt gtggtgcata ttttttcata taaaaacagt ttgcattaaa
      301 agtgccaaag ataagcattt tttttgccta atacttgcac aaggactaaa aactaattaa
      361 ttttgtcgtc ctattttttt aatcggggtg tagcgcagat ggtagcgtgc tacgttcggg
      421 acgtagaggt cgcaagttcg aatcttgtca ccccgacaaa ataaaatgga agttgcattt
      481 ttataaatgc taactttatt ttatttcagc gcgggatgta gctcagcccg gttagagtac
      541 gcgtctgggg ggcgcgtggt cgcaagttcg aatcttgtca tcccgacaat aaataaagac
      601 cggttgtttg atttttcaaa tgattggtct ttattatatt ctgcaatttg ttctgctttt
      661 taatttttac gtacatatat atttggcaat aaataattgc tacatttgct tcgtgaagaa
      721 tagcactcct aaatattacc gattttataa tactgctacg ctcaaatata gtagtttaag
      781 cttattgttt tgcatcacac tatgttttgc actactaagt tcatactctg ttgaagacaa
      841 aaagcaagac aatatccccc aacctaatac cggctttatt tgggaaacgc agaatctacc
      901 aatacaatat ctatgtacac caacacgaca aataataata tcagacggta gacactctgt
      961 caattacgga ttctctgcat tttatttatt gaaaacgaac acactaaaga ttgcacaaag
     1021 aatcagtaaa atgacaaagc ctgtttttct gcctgaacta gcagagttaa aaacctgctt
     1081 attataccca tttcatggtt tttggtagaa gaagtcctct ttctttaata tgatgttcgc
     1141 acccccaaat taataaacta ctaaaaatac aatcatgggt acaactattg ttggagttat
     1201 tctattagca ttatttattg ttcctttcgc agtattacaa ataagtaaac atagcaaaaa
     1261 aaagaaggat aaaaaagata atgaacctgc ataaaataat aagccctcgg aactccgagg
     1321 gcttattatt ttatatgtac tatttaaaat ctttgcactg ctaaaattta tcactaatcc
     1381 aatcaaacgc agattcaata ggattcggtt tagatcctag agattcgggt tttgcaggtg
     1441 ccccaccagg ttctactgtt gaaccatacc aatccattat atcatgttca acctccatta
     1501 caataacnnn nnnnnnntcc ttacaagccg cgcccaagga gaagtgaccg ttaacttcag
     1561 gcttaatata ctttttcata actcttattt ctttattaat ttaatcgttt gattgcctac
     1621 cttaatccaa tacatacctt tctcgataac aggtaaagct aaattagaac catcagactc
     1681 accggagtaa agagtacgaa tactattacc tacagcgtat gaagaaaatt cactcatatt
     1741 cttaatatac aatacatttc cttctatgta tgcaaataca ccattcccca tattttctga
     1801 taccacagta gtaatgtcat cggcagcttt tataagaagt tcgtaattag atattagtcc
     1861 tttgctggaa gcgaacgaat attcaccatc attattgaaa ttatgcactg caacaccatc
     1921 tttcaacaaa tacaatgtag aaccatcgtt aggaatttta tttgtattta gatacaaact
     1981 ataattacct gcagcagggg catacatacg gaatgctaaa tgtgcagcac catcttttag
     2041 ttttgcttca taaacgctta agttcatatt gtcataatca acacaccata actgagcagt
     2101 tgaagcagat gttggggttc ccattttttc tacatctttt cctattacat aagattcact
     2161 agctgattca ttagcggata caaataattg gtctgacatt tcaccgcccg cagttgctat
     2221 ccttaggttg tatatactgt taatattttc tgaagcgcca cgtaatgaat tacgggttct
     2281 ttttgttaaa tcaagcacag acgacattcc atttccttgt tgaataaaga aaggaacacc
     2341 tactacaaaa gactcatccc aaagagatac tgttaaatac tcttttgcgc cgtttttata
     2401 aacttgagca aacataacat ccgaatttac atctacgtgg aataattgtg tatttgctaa
     2461 accattccaa cccgtatctt taatttttgc agaagcaaac tcactaactg ataaagttgt
     2521 ttgacttgaa acaagtggag atccttctgt ttttacaaaa cgtacgtcat tataaccatt
     2581 tgatgcaaaa atatagaact tacccggttg caatgtagac ttaacatcag tcataaccca
     2641 accagaacca gtagaagcac gttttgcacc atcatattct tgtacataat attgatcacc
     2701 atataccatc tttataatct gatcacctga cttacgatat atgccatttt ctaaacttac
     2761 agggaatgga acactaaatg cataccattt cttaggtgat acggtgcctg acggatctaa
     2821 acttttatcc atgcttacag aacctaaaac tgttaaatgg ccaccatctt cgcgaaattc
     2881 gcctgatgca ccattttgtg cttgaatatt aaatgatgct acagtaatgt cgccatttaa
     2941 taccaagcta ccgtttgcgt ttactattaa attattaata gagtaatttc caccatctaa
     3001 cactaatgag gtttttatta ccaaattttc ttctacatcg ttcaataaaa ccacatcttt
     3061 gtttgaagca ttattgacag cctcagttaa tgttgaataa actttgtcgt ctattacaag
     3121 tgcaacacca gtataattag cattaattga aacta
//
LOCUS       DAOPID010000183         3150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_42753_length_3150_cov_3.664297, whole genome shotgun sequence.
ACCESSION   DAOPID010000183 DAOPID010000000
VERSION     DAOPID010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42753_length_3150_cov_3.664297"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="PK641_06050"
     CDS             <1..312
                     /locus_tag="PK641_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPG55941.1"
                     /translation="TLVSISDKQITVQSIRDVVCNYFQISVNSLISESRKREVVQARQ
                     IAMYFAKQMTKDSLTSIGNVIGKRNHATVLHACKTVKDLIDTDKSFRYSIEEIERKLN
                     A"
     gene            319..885
                     /locus_tag="PK641_06055"
     CDS             319..885
                     /locus_tag="PK641_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55942.1"
                     /translation="MIYKFILISDEVDGFMREISIDSEAKFSELHNAILDSVGYAKDV
                     ITSFFICNDDWEKQQEVTLLEMDTSADVDNYVMDETVISEFVSEEGQKLLYVFDYLSD
                     RAFFIQLKEIIPGKDLEIAACTASTGNPPEQSALVNFDDVKMPSSSTTDPSSIDENFY
                     GDENFDIEELDADSFSDMNFDEDFDALK"
     gene            882..1787
                     /gene="miaA"
                     /locus_tag="PK641_06060"
     CDS             882..1787
                     /gene="miaA"
                     /locus_tag="PK641_06060"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPG55943.1"
                     /translation="MKTLLVLLGPTGVGKTALGIKIAQHFSIPVISADSRQIYKGLKI
                     GTAAPSFEEQKLAQHFFIDTKELDELYSAGQFEIDVINLLPELFKKSDFALMVGGSMM
                     YIDAVCNGMDDIPNADPEVRKNLNRIYENEGIESLQAMLKLLDPEHYQRVDLKNYRRV
                     IHAVEVCFLTGRPYSEILTTPKKKRDFNIIKIGITRPTEDLYNRINHRVDAMIAEGLE
                     AEAADVYKQKQLNALNTVGYKEWFAYWDGEIESKEKVIELIKRNSRHYAKKQLTWFKR
                     DKSIKWFTPDDYQLIINHIEDICKN"
     gene            1775..2458
                     /locus_tag="PK641_06065"
     CDS             1775..2458
                     /locus_tag="PK641_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55944.1"
                     /translation="MQKLVILIITLLTYSHSINAQGSLSAYCTVGNNYIENTYVEIDA
                     IGAYKYNNWNFSLGVGLTTAPNHTAYFHALLADASYQFNIPKFPMRLGCKYLFNPHTY
                     SGLREHDWAIIVSYIPKHFDVELGYMIRYNYAANSGYGEYCNVLYNFAGYIWSRDNYY
                     NIRISLKNFDNLYFGRSTDIMLDIKLSYAYPRNLSYFIQGQCRAAGIGNIQFNHFDCK
                     IRAGIIWEI"
     gene            2446..>3150
                     /locus_tag="PK641_06070"
     CDS             2446..>3150
                     /locus_tag="PK641_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPG55945.1"
                     /translation="MGNIKLYISIIIAIVLCSCEFDPLDFIRTTYDVDKRTADSIKKN
                     EQNPVLNITTTSDNYSFIIASDLHLHTDEVMMPEFFSMASNSDASFVVINGDIYNSKS
                     EFASFAKDQFDNFNAKPCYFVAGNHDIYFDWKLYLDNFGSSTYSFKVQTPTTQDLYIT
                     LESSSATLGKTQLDWLKEQLNTRNQYRYCIIVTHVNFFPQQVNNGIYQQEELHVLFKL
                     FSDNNVNAVITGHSHDT"
BASE COUNT         1095 a          516 c          565 g          974 t
ORIGIN      
        1 acattggtat ctatatcaga caaacagatt acagttcaat ccatacgcga tgtcgtatgt
       61 aactacttcc aaatttccgt taatagtttg atatctgaat cacgcaaaag agaggttgtt
      121 caagcgcgtc aaatagctat gtattttgca aaacaaatga caaaagactc gcttacatca
      181 ataggtaatg ttatcgggaa aagaaatcac gcaactgttt tacatgcatg caaaacagtt
      241 aaagatctta ttgacactga caagagtttc cgctatagca tagaagaaat tgaacgtaaa
      301 cttaatgcat aattctgtat gatttacaaa tttatactca tttctgacga agttgatgga
      361 ttcatgcgcg agataagcat tgattccgaa gctaaatttt ctgagttaca taatgcaatc
      421 ttagattctg taggatatgc aaaagatgtt attacatcat tctttatatg taatgatgat
      481 tgggaaaaac agcaagaagt tacattgctt gaaatggaca caagtgccga cgtagataat
      541 tacgtaatgg atgaaacagt aatcagcgaa tttgtttcag aagaaggaca aaaattgcta
      601 tacgtctttg attatctatc agacagagcc ttctttatac aacttaaaga aattatcccc
      661 gggaaagatt tggaaattgc tgcttgcacc gcatcaaccg gtaatcctcc tgaacaatct
      721 gctcttgtaa attttgatga cgtaaaaatg cctagttcat cgacaacaga tccgagttct
      781 attgatgaga acttttatgg agacgaaaat tttgatatag aagagttaga tgcagatagt
      841 tttagtgaca tgaattttga cgaagacttt gatgcactaa aatgaaaaca ttgctcgtgt
      901 tgcttggccc cacaggtgta gggaaaacag cattgggaat aaagattgcc caacactttt
      961 ccataccggt aatttcggct gactcacgac agatatataa gggacttaaa ataggaacgg
     1021 ctgcaccaag ttttgaagaa caaaagttgg cgcagcattt tttcatagat acaaaggaac
     1081 ttgatgaatt atacagcgca gggcaatttg aaattgatgt aattaatttg cttcctgaat
     1141 tatttaaaaa gtctgatttt gcactgatgg ttggtggctc aatgatgtat attgatgctg
     1201 tttgcaatgg catggacgat atccctaatg cagatcctga agtaagaaaa aatctaaaca
     1261 gaatatacga aaacgaaggc attgagagtt tgcaagccat gctaaaattg cttgatcctg
     1321 aacattacca acgagtcgat ttaaaaaatt atcgcagagt aattcatgca gtagaggtat
     1381 gttttcttac cggacgtccc tattctgaaa ttcttaccac accaaagaaa aaacgtgatt
     1441 tcaacataat taaaataggc attactcgac ccactgaaga tttatacaat cggataaacc
     1501 atagagtaga tgctatgata gcagaagggc tggaagcaga agctgctgat gtgtataaac
     1561 aaaaacaact taatgctctt aatacagttg gatataaaga gtggtttgca tactgggatg
     1621 gcgaaataga atcgaaagag aaagttattg aactaattaa gcgaaattca cgtcactatg
     1681 ctaaaaaaca acttacatgg tttaagcgtg acaaatctat aaaatggttt actcctgatg
     1741 attatcaatt aataatcaac catatagaag atatatgcaa aaactagtta tactaattat
     1801 tacattattg acatacagcc atagcataaa cgcgcaaggc agtttgtctg catattgtac
     1861 tgttggtaat aactacattg agaatactta tgttgagatt gatgctattg gtgcttacaa
     1921 atacaataac tggaattttt ctttaggtgt cggacttaca actgctccta accatacggc
     1981 atactttcac gcactgctag ctgatgcatc atatcaattc aacataccaa agtttcctat
     2041 gcgtcttggc tgtaaatact tatttaatcc acacacatat tccggtctta gagaacacga
     2101 ttgggctatt attgtcagct atattccaaa gcactttgat gtagaattag gatatatgat
     2161 tagatataat tacgctgcta attctggata tggtgagtac tgtaatgtat tatacaattt
     2221 tgcaggttat atttggagtc gagataatta ttataacata cgaatcagtc taaagaattt
     2281 tgacaactta tattttggga gaagtacgga tattatgtta gatattaaat tatcatacgc
     2341 atatcctcgt aatttgagct attttataca aggccagtgt agagctgccg gaataggaaa
     2401 tattcaattc aatcattttg attgcaagat tcgtgcaggt attatatggg aaatataaag
     2461 ttatacatat caattataat agctattgtg ctatgctcgt gcgaatttga tccattagat
     2521 tttattcgta ccacgtatga tgtagataaa cgaactgccg attctattaa aaaaaacgaa
     2581 cagaatccgg ttttaaacat cacaacaaca agcgataatt attcatttat cattgcaagt
     2641 gacttgcatc tacatacaga tgaagttatg atgcctgaat tcttctctat ggcatccaat
     2701 tctgatgcaa gttttgttgt cattaatgga gatatataca atagtaagtc tgaatttgct
     2761 tcatttgcca aagatcaatt tgacaatttt aatgctaagc catgctattt tgtagcaggc
     2821 aatcacgata tctattttga ttggaaacta tatctcgata attttggaag ttctacatat
     2881 agtttcaagg ttcaaacgcc taccactcaa gatttataca taacacttga aagtagtagt
     2941 gctactcttg gaaagacaca attagactgg ctaaaagaac aacttaatac tcgtaatcaa
     3001 tatcgatact gcataatagt tacacatgtc aattttttcc ctcaacaagt taataacggt
     3061 atctatcaac aagaagaatt gcatgtacta tttaagttat tctcagacaa taatgtaaac
     3121 gcagttatta caggtcactc tcatgataca
//
LOCUS       DAOPID010000184         3150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_42758_length_3150_cov_3.472698, whole genome shotgun sequence.
ACCESSION   DAOPID010000184 DAOPID010000000
VERSION     DAOPID010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42758_length_3150_cov_3.472698"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="PK641_06075"
     CDS             complement(<1..686)
                     /locus_tag="PK641_06075"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPG55946.1"
                     /translation="MIIRDWTSDYKELRNATNNFVKKDNIAKLHQIYKNCDGVSSLFQ
                     KRSTNALKIAYIAVDEIGLGNSAVIAILLYPLYAEKKIDAASIEKQFGNETAVIVGGL
                     NRIQQLYTKTSAMESENFRKLLLTFAEDVRVIFIMLSERLFEMRNLKEREAEIQQNIA
                     RETAYLYAPMAHRMGLYKIKTELEDLSLKFTAPDTYKTIAQKLSESKRSRDAYIEIFI
                     GPLNEKVAAAG"
     gene            complement(683..1294)
                     /locus_tag="PK641_06080"
     CDS             complement(683..1294)
                     /locus_tag="PK641_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55947.1"
                     /translation="MKRLRYTASIIVYLIMILCLNSCLETKHFEVDESLLEEQINDND
                     IQISYRMPSGFKPIKKSMVDSIASVELQHDPFAAKILAIYVDTLLRNASAVVSDMRNI
                     PAERTENKLDFYFTEFNPNNYWDKVEISRYKFNQFPKVVQLDMYNSNRRLIKLFFYDE
                     NKVKFSVDYVLPEEYYLDIKPFIESSIGSFNEGYTIVIEQTKQ"
     gene            complement(1340..>3150)
                     /gene="alr"
                     /locus_tag="PK641_06085"
     CDS             complement(1340..>3150)
                     /gene="alr"
                     /locus_tag="PK641_06085"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPG55948.1"
                     /translation="FRNSDTLIFRKGYGVENAIKQINPNIKLFSWNMDDENATLNITS
                     INIKLNNTIIGYKYKNVEGSTILPFSDNASIENGIHCLACCLLIGIKTSLENLERISM
                     RLELKQGKNNCLLIDDAYNSDINSLTIALDFLQANAENKYEKRTVIISDLSQTGEIDD
                     ILYEKVISLINEKKITRVIGIGDKIAKYWHRFPFESRFFVNANEMLHSNISFQDEIIL
                     IKGARKYHLEELVSALELKRHETVMEIDLNALVHNFKKLRSYLKPETKTVAMIKANGY
                     GCGAIEVAQTLSHHHCDYFGVAVADEGVELRQAGIRTNIMIMNPEQSSFGLLLQYRLE
                     PEIYSFNILHAFDEYADKQGVKNFPIHIKIDTGMHRLGFSPEEIDELIKDLSKMPSVK
                     VDSAFSHLAAADDPNMDTFTLNQIDAFMNATDKLRANLKYPFLRHILNSYGVQRFDKY
                     QFEMVRLGIGLYGIGFNEKSDIKNIATLKTVILQIKHIKKDETIGYNRKGVLVRDTTI
                     AAIPIGYADGLNRAFGNRNGYAIVNDKPAPFIGNICMDVCMIDITDIPNVKEGDSVTI
                     FGDDLTISDLAQKTHTIPYEILTGISLRVKRVYFQE"
BASE COUNT          931 a          539 c          489 g         1191 t
ORIGIN      
        1 cctgctgctg caactttttc atttaatggg cctataaata tttcaatata agcatcacgc
       61 gaacgtttac tttcgcttaa tttttgtgct atagttttat aagtatcagg agcagtgaat
      121 tttagagata aatcttcaag ttcggttttt attttatata gtcccattct gtgagccatg
      181 ggagcatata aatatgccgt ttcgcgtgct atgttttgtt gaatctcagc ttctcgttct
      241 tttagatttc gcatttcaaa caaacgttcg gagagcataa taaatattac tcgaacgtct
      301 tctgcgaatg tcaacaatag ttttctgaaa ttttcagatt ccattgccga tgtttttgta
      361 tatagttgct gtattctgtt taaaccacca acaataactg cagtttcatt tccaaattgt
      421 ttttcgattg atgccgcatc aattttcttt tctgcgtaga gtgggtataa taggattgct
      481 attactgctg aattacctaa tccgatctca tctacagcta tataagcgat ttttaaagca
      541 tttgtactgc gcttttgaaa caaagaacta acgccatcgc aatttttgta tatttgatgt
      601 aatttagcaa tgttgtcttt cttgacaaaa ttatttgtgg cattgcgtaa ctctttgtag
      661 tctgatgtcc aatctcttat tatcattgtt tagtttgttc tataactata gtgtatccct
      721 cattaaatga tccaatagaa gactcaatga atggtttaat gtcaagataa tattcttcgg
      781 gtaaaacgta atctacgcta aatttgactt tgttttcatc ataaaaaaac aatttaataa
      841 gtcttctatt ggaattatac atatccagtt gaacaacctt tggaaattga ttgaatttat
      901 atcttgaaat ttcaacctta tcccaataat tattagggtt gaattctgta aaataaaaat
      961 ctaatttatt ttctgtacgc tcggccggta tattacgcat atcagataca actgcactag
     1021 cgttgcgtaa taatgtatca acatatattg ctaatatttt agctgcaaat gggtcgtgtt
     1081 gcaattcgac cgatgcaata gaatcaacca tactcttttt tataggttta aaacctgacg
     1141 gcatcctata tgatatttga atatcattat cgtttatttg ttcttccaat aatgattcgt
     1201 ctacttcgaa atgctttgtt tctaaacatg aatttaagca taatatcatt attaagtata
     1261 caataattga agctgtgtat ctgagtcttt tcatatcaca aatattttat tcgctagctg
     1321 caaatataat aaaaaaatgc tattcttgga agtaaacgcg cttaactctc aatgatatac
     1381 cggttaatat ttcgtaaggt attgtatgtg ttttctgtgc taaatcgctg attgttaaat
     1441 catcaccaaa tattgttaca gaatcacctt cctttacatt tggtatatca gtaatgtcta
     1501 tcatacatac atccatgcaa atattgccta taaatggtgc cggtttgtcg tttacaattg
     1561 catatccgtt tctattaccg aatgctctgt tcaagccatc tgcatatccg ataggtatgg
     1621 cagctattgt cgtatcacgc acaagaaccc cttttctgtt atagccaatg gtctcgtctt
     1681 tctttatgtg tttaatttgt aatatcacgg tttttagggt ggctatattt tttatgtctg
     1741 atttctcatt aaaaccaatt ccatataaac cgatgcctaa tctaaccatt tcgaattgat
     1801 acttatcaaa acgttgtact ccgtatgagt ttagaatgtg tcttaagaat ggatatttaa
     1861 gattggctct taatttgtcc gttgcattca taaatgcatc gatctgatta agtgtaaatg
     1921 tatccatgtt aggatcatcg gccgcagcta agtgagaaaa cgctgaatca acttttacag
     1981 aaggcatctt actaaggtct ttaatcaact cgtcaatttc ttcgggtgag aagcccaagc
     2041 ggtgcatacc tgtatctatt ttgatgtgaa tagggaagtt tttaactcct tgtttgtctg
     2101 catattcgtc gaatgcatgc aatatattaa aactatatat ttcaggttcc aatctatatt
     2161 gtaacaataa accaaagctg ctttgctccg gattcataat cataatattt gtacgtatac
     2221 cggcttgacg cagttcaaca ccttcatctg ctactgcaac accaaaatag tcgcaatggt
     2281 ggtgacttaa agtttgagca acttctattg ccccacagcc gtagccatta gctttaatca
     2341 ttgccactgt ttttgtctca ggctttagat atgagcgaag tttcttgaag ttatgaacta
     2401 atgcatttaa atcaatttcc atcacggttt cgtgacgttt taattctaat gcagagacta
     2461 attcttcaag gtggtatttt cgagcacctt ttattaagat tatttcatct tgaaaactta
     2521 tattagaatg tagcatttcg ttagcgttta cgaaaaatct actttcaaac ggaaaacgat
     2581 gccaatattt agctatttta tctcctatgc ctataactct tgtaattttc ttttcgttta
     2641 ttagagatat tactttctcg tataatatat cgtcaatttc gcctgtttga cttaaatcag
     2701 atataattac agtacgtttt tcatatttat tttcggcatt tgcttgtaaa aagtccaaag
     2761 caattgtcaa tgaatttatg tctgaattat atgcatcatc aataagtaag caattgtttt
     2821 ttccttgttt caattcaaga cgcatactaa tgcgctctaa attttctaat gatgttttta
     2881 tacctattaa taaacaacac gctaagcagt gtataccgtt ttcgattgat gcattatctg
     2941 aaaaaggaag gatggtagaa ccttcaacat ttttgtattt atagcctatt attgtattat
     3001 ttaatttaat attaatggat gtgatgttta gagttgcatt ttcatcgtcc atattccatg
     3061 aaaataattt tatattaggg ttgatttgtt taattgcatt ttcaacacca tatccttttc
     3121 taaaaatgag agtatcagaa ttgcgaaata
//
LOCUS       DAOPID010000185         3139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_42938_length_3139_cov_3.768482, whole genome shotgun sequence.
ACCESSION   DAOPID010000185 DAOPID010000000
VERSION     DAOPID010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42938_length_3139_cov_3.768482"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..1247
                     /locus_tag="PK641_06090"
     CDS             18..1247
                     /locus_tag="PK641_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPG55949.1"
                     /translation="MYNIIFFIIIAFLILNFVSEQWVSYLNAKCFGKPIPSILKGIYD
                     EEKYQKQQAYSKENYRFSLLSSSVSMLIIFIALFVGFFGWLDTYLRLYISNEVFLPLA
                     FFAVLYVINEIIDTPFEYYQTFAIEDKYGFNKTTKKIFISDKFKNLLLSAVIGGVLLG
                     LIVWFYTLIPDYFWLLAWLVISLFTIFILMFYSEWIVPMFNKQTPLEEGDLRTKIEEF
                     ATKAGFTIKNIYVIDGSKRSTKANAYFSGLGSKKRIVLYDTLIKELSSEEIVAVLAHE
                     IGHYTKKHTLSMLFVSLLSNLFMLWLLGFFLANPLLSQAMGGSEPSFHLGLLAFGILY
                     TPISVLLGLAVNAFSRKHEYQADAYAKSFGLSQALISALKKLSETSLSNLQPHKAYVF
                     MHYSHPTLLQRIEALQS"
     gene            1244..1699
                     /locus_tag="PK641_06095"
     CDS             1244..1699
                     /locus_tag="PK641_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008155516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HPG55950.1"
                     /translation="MNTEFTISTAESCTGGNISHLITMVAGSSSYFKGSVVSYANEVK
                     INVLGVPESYINQFGAVSKVVVEAMANGVRKLMDTDYAVATSGIAGPDGGSEDKPVGT
                     VWVAICNRKEVKSRCLHLSGNRIENIRDTSKIVLQWLRDEYNLSLPEGE"
     gene            complement(1679..2662)
                     /locus_tag="PK641_06100"
     CDS             complement(1679..2662)
                     /locus_tag="PK641_06100"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG55951.1"
                     /translation="MEKKYEILSTRKLVIGYKKKELLPPQNLSLYRGEMVCLIGQNGC
                     GKSTLIRTLSGLQVAISGDVTIKEKKISQLNNAAKAKLIGLVLTDSTEIRSLTVRDMV
                     SMGRFPHTNWFGKLNNKDEDIISQSIEKVHLTNKSDNYFSELSDGEKQRTLIAKALAQ
                     DTPIIFLDEPTAHLDLPNKIDIMLLLRHLSNDMQKTILLSTHELELAIQTADKIWMMQ
                     SSGIQKGVPEDMILENKIQSNFYSKQFSFNNKTGGFEIKYTGNKTICLNGDDPVLLFW
                     TKRALLRAGYVLTEDAYTRITVTEKPEWIIYRDNKTEKRYNIENLLFTLRQ"
     gene            complement(2665..>3139)
                     /locus_tag="PK641_06105"
     CDS             complement(2665..>3139)
                     /locus_tag="PK641_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005775944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HPG55952.1"
                     /translation="YIVWTMGSLSSVTWNYMYVMAPILLFGMLISFSLQKKLNAMLLG
                     ENYARGLGISVFWTRLAIIFATCLLAGASTAFTGPIAFIGIAVPHIIRGIFKTSDHRI
                     ILPGCIIGGAALLLICDIASQLPGTGYSLPINSISAIVGAPIILWVILKNKHLHI"
BASE COUNT          977 a          497 c          542 g         1123 t
ORIGIN      
        1 aatataaaat aatagctatg tataatataa tattttttat cattattgca tttttgatac
       61 tcaattttgt atcagagcaa tgggtgtcat atctaaacgc taaatgtttt ggaaaaccga
      121 taccatctat tttaaaaggt atatatgatg aagaaaaata tcaaaaacag caagcgtatt
      181 ctaaagaaaa ctacagattt tctttattat cttcttctgt atcaatgcta ataatattta
      241 ttgcactatt tgtaggattt tttggatggt tagatactta tttaagatta tatatatcaa
      301 atgaagtatt cttgccttta gctttttttg ctgtcttata tgttataaat gaaataattg
      361 atacgccatt cgaatattat caaacttttg ccattgaaga taaatacgga ttcaataaaa
      421 caacaaaaaa aatatttata tcagataaat ttaaaaatct attattatcg gcagttatcg
      481 gtggtgtttt gttaggtctt atagtttggt tttacacgct tattcctgac tatttttggt
      541 tattggcttg gttagtaata agtttgttta caatctttat tttgatgttt tattctgagt
      601 ggattgtccc aatgtttaat aaacagactc cattagagga aggcgattta cgtacaaaaa
      661 ttgaggagtt tgcaactaaa gcaggcttta ccataaaaaa tatatacgtt attgatggat
      721 caaaaagaag tacaaaagct aatgcgtatt ttagtggctt aggttcaaaa aaacgtatag
      781 tattgtatga tactttgata aaagaattga gttcagaaga aattgtggca gtattagcac
      841 acgagatagg tcattatacg aaaaaacaca ccttgtcaat gctatttgta tcattattgt
      901 caaatctatt tatgttatgg cttcttggat tctttttagc aaatccttta ttgtcacagg
      961 caatgggagg ctcagaacct agtttccatc ttggtttgtt ggcatttgga attttatata
     1021 caccgatttc agtgttatta ggacttgcgg ttaatgcatt ttcaagaaaa catgaatatc
     1081 aagctgatgc atacgcaaaa tcttttggtt tgtctcaagc tttgatttca gctttgaaga
     1141 aattatctga gacatcattg tctaatttgc aaccacataa agcctatgta tttatgcact
     1201 attctcatcc tactttgttg caaagaatag aagcactaca gtcatgaaca ctgaatttac
     1261 aatatctaca gccgaaagtt gcacaggagg aaatatttcg catctcataa ctatggttgc
     1321 aggaagttct tcttatttta aaggctctgt tgtctcgtac gcaaatgagg taaagattaa
     1381 tgttctagga gttcctgaat catacattaa ccaatttggt gctgtaagta aagtcgttgt
     1441 tgaagcaatg gcaaatggtg tacgtaaatt gatggacacg gattacgcag tagctacttc
     1501 gggaattgca ggacctgatg gcggttctga agataaacct gttgggactg tttgggttgc
     1561 aatctgtaat aggaaagaag ttaaaagtcg ctgcttacat ttgtcaggaa atagaataga
     1621 aaatattagg gatacttcta aaatagtatt acaatggctt cgtgatgaat ataatttatc
     1681 actgcctgag ggtgaataat aagttttcta tattgtatct tttttctgtt ttgttgtctc
     1741 tatagataat ccattcaggc ttttctgtaa cagttatcct agtgtatgca tcttccgtaa
     1801 gtacgtatcc ggctcttaat aatgcacgtt ttgtccagaa tagcaaaacc ggatcatctc
     1861 cattcaaaca aatagtttta tttcccgtat attttatttc aaatccacct gttttattat
     1921 taaaagagaa ttgttttgaa taaaaattcg attgaatttt gttttccagt atcatgtcct
     1981 ccggaactcc tttttgtata cctgacgatt gcatcatcca tattttatct gctgtttgaa
     2041 ttgccaattc caattcgtga gtagataaaa gaatcgtctt ttgcatatca tttgataagt
     2101 gtctcaacaa caacattatg tctattttgt taggcaaatc aaggtgtgca gttggctcgt
     2161 ctaagaaaat aataggagta tcttgcgcca atgctttagc aataagagtc ctttgttttt
     2221 ctccatcaga taattcagaa aaataattat cacttttatt tgtaagatgc actttttcaa
     2281 ttgattgaga tataatatct tcgtctttat tattcaattt accaaaccaa ttggtgtgag
     2341 ggaatcgccc catacttacc atgtctcgca ctgttaatga ccttatttcg gtagagtcag
     2401 ttaatacaag tcctattagt tttgcttttg ctgcattatt cagttgagat atttttttct
     2461 ccttgatagt cacatcaccg gatattgcaa cttgtaaacc tgaaagagtt cttattagag
     2521 ttgattttcc acatccgttt tgtccaatca gacagaccat ttctcctctg tacaaactta
     2581 aattttgagg aggtagcaac tcttttttct tataaccgat aacgagtttg cgagtgctta
     2641 atatttcata ttttttttcc aatgtcagat atgtagatgt ttgtttttaa gtataaccca
     2701 taaaattata ggcgcaccaa ctattgcgct aattgaattg ataggtaaac tgtatcctgt
     2761 accgggcaat tgtgatgcga tatcgcatat aagtagtagg gcagcgccac ctattataca
     2821 tcccggtaat ataattctgt ggtctgatgt tttaaaaatg cctcttatga tatgtggaac
     2881 tgcgatgccg ataaatgcga tcggtcctgt aaatgcggtt gatgcaccgg ccagtagaca
     2941 tgttgcaaaa attatcgcca aacgtgtcca aaatactgat atacctaatc cacgagcata
     3001 attttctcct agaagcatcg cattaagttt tttctgtaag gaaaatgaaa taagcatacc
     3061 gaataataaa attggagcca ttacatacat gtaattccat gttacagaac ttagactgcc
     3121 cattgtccaa actatgtat
//
LOCUS       DAOPID010000186         3121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_43296_length_3121_cov_4.291259, whole genome shotgun sequence.
ACCESSION   DAOPID010000186 DAOPID010000000
VERSION     DAOPID010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43296_length_3121_cov_4.291259"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PK641_06110"
     CDS             <1..630
                     /locus_tag="PK641_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55953.1"
                     /translation="YDPELFYPEKLATERFVICYTGRIIGTDMQDPTLLFESLKKMND
                     SNIINPETLVVRWYVDDASANIIKQFANKYRVKSYMDIKTYIPVSEIPSVLKSSSIIL
                     VLTNKQTVYGPKGIMTTKFFEALATCKPILCVRSDEAGLSNIIKETNCGIAATTTNEV
                     CNFIDKWQEYWKTNSYTIIEQDSDKISVYSRKLQAKQFSDLFDKLIKNV"
     gene            656..1675
                     /gene="murB"
                     /locus_tag="PK641_06115"
     CDS             656..1675
                     /gene="murB"
                     /locus_tag="PK641_06115"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007845527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HPG55954.1"
                     /translation="MKTYENYSLLKNNTFGIDVKANYFIEYDSVEELQQLLNSDLLSE
                     NKFLQIGGGSNLLFINDYQGVVLHSQIKGIDAVEGTRDTVVVRAGAGEVWDDLVAYCA
                     QKNYGGIENLSGIPGEVGATPIQNIGAYGTEVKDVIDKVECVNVSSSEERIFTNAECK
                     FGYRDSIFKRELKNKYIVTAVHYKLRKKNWDLNVNYGSLADEIKNYDEISIQNIRKAV
                     LDIRKKKLPEPKVWGNAGSFYMNPVISSKEYENLLKIYPDMPCYKLSNDEVKVPAGWL
                     IEQCGWKGKSIGNVAVHGSQALVLINKGGANGLDIINLSDSIRHSIRERFGIDLIPEV
                     NFIFG"
     gene            complement(1762..>3121)
                     /locus_tag="PK641_06120"
     CDS             complement(1762..>3121)
                     /locus_tag="PK641_06120"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPG55955.1"
                     /translation="AAKILVQRIKDTVGDEPVFLTGDFNSAEGDAVTQWLKSGSDNTL
                     KFRDSYRDIDPSGNVTTGFGVKFDYIYYPNSDKYSCTSSWVVTNPAGASDHMPIVANV
                     KYSGSSNPVVVESVSFKDIKTTYESDDTYTLDVDYVAKENRDVYLCMYDNAGTWNVIG
                     EVHKAVTEGSGSLRFVMPVDPKPAVGEGYIFKCDIRPTGGAWDSFIDMKLAEDITITV
                     NEDELDFSGCNIMDQIDAEISYYAPDWNETHTASASVENNVVEFSFPTATTDLWMSQA
                     KIRSANGINLTEGVQYTLSCTITSNVNIADFDIKVEEKVNSFFGKANVALQAGVPCKI
                     KVSETAAALSNAYIIIGTGGHGDNSIISISNIILRESSCEGVNTDINEEESENISIYP
                     NPVTDVLHINSSNEEEKHNVINTNGIQVLQTIGNNVNLSGLSSGLYFINVQDKYYRFI
                     KQ"
BASE COUNT         1019 a          492 c          570 g         1040 t
ORIGIN      
        1 tatgatcctg aactgtttta tccggaaaaa cttgctacag aacgctttgt aatatgttat
       61 acaggtcgaa ttataggtac tgacatgcaa gatccgactt tgttattcga atcattaaag
      121 aaaatgaatg attcaaatat aattaatccg gaaacattag ttgtgcgttg gtatgttgat
      181 gatgcatcgg caaatataat taaacaattt gctaataaat atcgagtgaa atcgtacatg
      241 gatatcaaaa catacatacc tgtgtctgaa ataccatctg tattaaaatc gagttcaata
      301 attttggtac tcacaaataa gcaaacagtt tatggaccta aaggcataat gactactaag
      361 tttttcgaag cattagctac ttgtaaacca atactttgcg tacgtagtga tgaagccggg
      421 ctgtcgaata ttataaaaga aacgaattgt ggaatcgctg caactacaac aaatgaagtc
      481 tgtaatttta tagataagtg gcaggagtat tggaaaacta attcttatac aataatagaa
      541 caagattcgg ataagattag tgtgtactca agaaagttac aagcaaaaca attctcagat
      601 ttgtttgaca aactgattaa aaacgtttaa ttttagggcg taataaaagc tatatatgaa
      661 aacttacgag aattattctt tattaaagaa caacaccttt ggtattgacg ttaaagctaa
      721 ttattttatt gaatatgatt cggtagaaga actacagcaa ttacttaata gtgatttact
      781 gtctgaaaat aagtttttac aaattggtgg tggaagtaat ctattattta ttaatgatta
      841 ccaaggagtt gtgttgcatt ctcaaattaa aggtatagat gctgttgaag gtacacgtga
      901 cactgttgtt gttcgcgcag gtgcaggtga agtttgggat gatttagtag catattgtgc
      961 tcaaaaaaac tatggtggta tcgagaatct ttcaggtata ccgggtgaag taggagcgac
     1021 tccgatacaa aatataggtg cttatggtac agaagttaaa gatgttattg ataaggttga
     1081 atgtgtaaat gtgagttcga gtgaagaacg catctttact aatgcagaat gtaaattcgg
     1141 gtaccgcgat agcatattta aaagagaatt aaaaaataaa tatattgtta cggcagttca
     1201 ttataagtta cgtaaaaaga attgggattt gaatgtaaac tacggtagtt tggctgatga
     1261 aatcaaaaac tacgacgaaa tttcaattca gaatataaga aaggctgtct tagatatacg
     1321 taaaaagaaa ttacctgaac caaaagtatg gggtaatgcc ggaagttttt atatgaatcc
     1381 cgtaatatca tcgaaagaat acgaaaactt gcttaaaata tatccggaca tgccttgtta
     1441 taagttatcc aatgatgagg taaaagtgcc tgcaggttgg ttgattgaac aatgcggatg
     1501 gaaaggtaaa tctataggca atgtagcagt tcatggaagt caggcattag tattaattaa
     1561 taaaggtggg gcaaatggct tggatataat caatttatca gattcgattc gccattcgat
     1621 acgtgaacgt tttggaatag atttaattcc cgaggttaac tttatatttg gttagttaat
     1681 tgtcatataa acggtgtatt taataaaaaa gtgagatgtt gtaaaaaaca tctcactttt
     1741 ctatttgtta accgacctat attactgttt aataaaacga taatatttat cttgtacgtt
     1801 tatgaaatat aatccgctcg ataatccgga tagatttaca ttattaccta tagtctgcaa
     1861 aacttgaatt ccgtttgtgt ttattacatt gtgtttttct tcttcattag atgaattaat
     1921 gtgtaaaacg tccgttaccg gattcggata tattgaaata ttttctgatt cctcctcgtt
     1981 aatatcagta tttacgcctt cgcaagatga ttctcttagt ataatattac ttatgcttat
     2041 gattgaatta tctccatgtc ctccggttcc gattatgata taggcatttg acaatgcagc
     2101 cgcggtttcg cttactttta ttttacatgg aactcctgct tgtaaagcta cattagcttt
     2161 tccaaaaaag gagtttacct tctcttctac ctttatatca aagtcagcga tgtttacatt
     2221 tgaggtaata gtacacgaaa gagtgtattg aacaccttct gttagattaa ttccattagc
     2281 agaccttatt tttgcctgac tcatccaaag atctgtagtt gctgttggaa acgaaaattc
     2341 tacaacatta ttctcaacag atgcagaagc tgtatgtgtt tcattccaat cgggagcgta
     2401 gtatgatatt tcagcatcta tctgatccat tatattacat cctgagaagt ctaattcgtc
     2461 ttcgttaact gtgattgtga tatcttcagc tagtttcata tcaatgaaac tatcccatgc
     2521 tcctcctgtt ggacgtatat cgcatttgaa tatatatcct tcaccaacag ccggtttggg
     2581 gtctacaggc atcacaaaac gaagactacc tgaaccttca gtaactgctt tgtgaacttc
     2641 tccaataacg ttccacgttc cggcattgtc gtacatacat agatatacat cacgattttc
     2701 ttttgcaaca taatcaacat caagtgtata tgtgtcatct gattcatatg ttgttttaat
     2761 atctttaaat gatactgatt ctactacaac aggatttgat gaacctgaat atttaacgtt
     2821 ggcaactatc ggcatgtgat ctgatgctcc tgcaggattt gtcacaaccc aagaagatgt
     2881 gcaactatat ttatcactat ttggatagta aatataatcg aatttaacac cgaatccggt
     2941 agttacattg ccacttggat ctatatcgcg ataactatct ctgaatttta aagtattatc
     3001 agagcctgat tttagccatt gtgttacagc atcaccctct gcagaattaa agtcacctgt
     3061 taagaatact ggttcatctc ctacagtgtc tttgatgcgt tgaaccaata tttttgcagc
     3121 t
//
LOCUS       DAOPID010000187         3106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_43569_length_3106_cov_3.941658, whole genome shotgun sequence.
ACCESSION   DAOPID010000187 DAOPID010000000
VERSION     DAOPID010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43569_length_3106_cov_3.941658"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1883
                     /locus_tag="PK641_06125"
     CDS             <1..1883
                     /locus_tag="PK641_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55956.1"
                     /translation="TADGYNPYKVTSEGIDWEIIEPENPWSNIGYWGDHQMIYLLKLM
                     ELSRKYYPEALGSSLNDLRYAFANVPYRLKSYSEIVADPKNSITFEDKLHNHIFDILP
                     AYGSDARLMLDKNDEVILASFTEKLLATLLAKLSNFIPEAGIWMNTLRPEWNDANNAL
                     VGYGASMVTLYYMRRFVAFSKNLFAESNENSFEVSAEMESFFNGLSAAFEGSASVVKN
                     GFNDKQRRVVVDALGKAGEAYRSQIYKGFTGKKQSISKAELVKFFETVLSYIDESIRI
                     NKREDGMYHSYNLVSFGEGTVSVNHLYEMLEGQVAVLTSMELSASEACSLLDSLRKSS
                     LYRADQNSYILYPNKRLASFLEKNNLPNDAVSLPVVKQLLAAKDGKILSEDHLGGVHF
                     NANFNNAGFLSAALDKLPTKVSEKDRQLLLDIYEKIFDHKSFTGRSGSFYKYEGLGSI
                     YWHMVSKLLLAIGENIQKAMSEGADANVIARLKAHYAAVKDGIGAHKSPENYGSFPFD
                     PYSHTPIMAGVQQPGMTGQVKEDIINRFFELGIFINNACLSINKDMLNYSEFIKADVP
                     YIKFTYCSIPFTYLKDGKEGIDVIYADKSTERIVTYALDQKLSARIFARDSDIKGLIV
                     HL"
     gene            1990..>3106
                     /locus_tag="PK641_06130"
     CDS             1990..>3106
                     /locus_tag="PK641_06130"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPG55957.1"
                     /translation="MKKYYIILAIIFIACFTACQKNEIKDTTDEKVEALLSKMTLQEK
                     IGQLNQRDAGDVSDEIIQNVKDGNIGSILNCDPEKVNELQRVAIEESRLGIPLLYARD
                     VIHGFKTVFPIPLGQACSWDTTIITEGARVAAAEAASTGIRYTFAPMIDVTRDPRWGR
                     IAESLGEDPYLTSVLGVAMIKGFQGSDMSAPDRVAACAKHFAAYGLTESGRDYNVVFT
                     SEQRLRDYVLPPFEAAAKAGVATFMAGFNELNGVPVSGSTFLLRDILREEWGYKGMVV
                     SDYFSIEQMAVQGFCENEAESAKLGMLAGVDMDMMSFVYVNYLDSLVQANAVDEKLID
                     DAVRRVLRLKFDLGLFDNPYTDLSKKDSVLYSEEHLRA"
BASE COUNT         1013 a          462 c          640 g          991 t
ORIGIN      
        1 caacagcaga tggctataat ccatataaag taacaagcga aggtattgat tgggaaataa
       61 ttgaacctga aaatccatgg tcaaatatcg gatattgggg cgaccaccaa atgatctacc
      121 tacttaaatt gatggaacta tctcgtaaat actatccgga agcattaggt tcctcactaa
      181 acgatttgcg ttatgcattt gcgaatgttc catatcgtct taaatcatat tctgaaatag
      241 ttgcagatcc aaagaactcc ataacatttg aagacaaatt acataatcat atttttgaca
      301 tattacctgc gtacggatct gatgcacgtt tgatgttaga taaaaatgat gaggtaatat
      361 tggcatcctt caccgaaaaa ttattggcaa ctctacttgc aaaattgagt aactttattc
      421 ctgaagcagg tatttggatg aacaccttac gtcctgaatg gaatgatgca aataatgctt
      481 tagtaggata tggtgcctca atggttactt tgtattacat gcgcagattt gttgcgtttt
      541 ctaagaatct atttgcagaa tcaaacgaaa atagttttga agtatcagca gaaatggaaa
      601 gtttcttcaa tggcttatct gctgcatttg aaggatctgc atctgttgtt aaaaatggtt
      661 tcaatgacaa acaacgtcgt gttgttgtag atgctttagg caaagccggt gaggcatatc
      721 gttcgcaaat atataaaggt tttacaggca agaagcaaag catttcaaaa gcagaattag
      781 ttaaattctt cgaaacagta ctttcttata ttgatgagtc aatacgaatt aacaagcgtg
      841 aagacggaat gtatcattcg tataatttag tttcttttgg agagggaaca gtttcggtaa
      901 atcacttata cgagatgctt gaaggacagg ttgctgttct tacatcaatg gaattgtcgg
      961 ctagtgaagc atgttcttta ttagattctt tgcgtaaaag ttctttatat cgtgcagatc
     1021 aaaatagcta tattttatat cctaacaagc gtttagctag ctttttagaa aaaaacaatc
     1081 tgcctaatga tgctgtttca ttacctgtag ttaagcaatt gttggcagca aaagacggta
     1141 agattttatc agaagaccat ttaggtggtg tgcattttaa tgcaaatttc aataatgcag
     1201 gctttttatc tgctgcatta gataaattac caactaaggt gtcagaaaaa gatcgtcagt
     1261 tattgctcga tatatacgaa aagatatttg atcataagtc atttacagga cgttcaggat
     1321 cattctataa gtacgaaggt ttaggttcta tttattggca catggtatca aaattacttt
     1381 tggcaatagg cgaaaatatt caaaaagcca tgtcagaagg tgctgatgca aatgttattg
     1441 ctcgtctaaa agcgcattat gctgctgtaa aagacggaat aggtgctcat aaatcgcccg
     1501 aaaattatgg ttcattccca ttcgatccat attctcatac tcctattatg gcaggagttc
     1561 aacaacccgg tatgacaggt caggtaaaag aagatatcat aaatcgtttc tttgaactgg
     1621 gaatcttcat aaataatgct tgtctgtcaa ttaacaaaga catgttgaat tattctgagt
     1681 ttattaaggc tgatgttccg tatattaagt ttacatattg ttcaatacca tttacatatt
     1741 taaaagatgg taaagaaggt atagatgtta tttatgcaga taaatcaacc gaaaggattg
     1801 taacttatgc tttagatcaa aaattaagtg cacgcatatt tgcacgcgat tcagatatta
     1861 agggactgat tgttcattta tagtaactgt atttgctcaa gaaatttaga gtatgtagat
     1921 gataattagg aggaatacag taaatataac tgtttgtcct ccttttattt aataataaat
     1981 tttatgtgta tgaaaaagta ttacatcata ttagcgatta tatttattgc ctgctttaca
     2041 gcatgtcaaa aaaatgaaat taaagataca accgatgaaa aggttgaggc cctattgtct
     2101 aaaatgacct tacaagaaaa gataggccaa ttaaatcagc gtgatgccgg tgatgtgtct
     2161 gacgaaataa ttcagaatgt aaaagacggg aatataggtt caatattaaa ttgcgatccc
     2221 gaaaaggtaa atgagttaca acgtgttgct atagaagaga gtcgtttagg aatacctttg
     2281 ctatatgctc gtgatgtgat tcatggtttt aaaacagtat ttcctattcc tttagggcaa
     2341 gcttgtagtt gggatactac aattattaca gaaggtgctc gcgtagcagc agctgaagct
     2401 gcatcaacag gtatacgcta tacttttgct ccaatgattg acgttactcg tgatccaaga
     2461 tggggtagaa tagccgagag tttaggtgag gatccatatt taacatcagt attaggtgtt
     2521 gcaatgataa aaggatttca aggatctgat atgtctgccc ctgatagggt tgcggcttgt
     2581 gcaaaacatt ttgctgccta tggtttaaca gaaagcggta gagattataa tgtagtattt
     2641 acttcagaac aacgtttacg cgattatgtt ttgcctccat ttgaagctgc tgcgaaagca
     2701 ggtgttgcta catttatggc cggatttaat gaattaaacg gagttcctgt atcaggaagc
     2761 acattcttgt tgagagatat attgagagaa gaatggggat ataaaggaat ggttgtaagt
     2821 gattatttct caatagaaca aatggcagtc caaggttttt gcgagaacga agccgaatca
     2881 gcaaagttgg gaatgcttgc cggagttgat atggatatga tgagttttgt atatgtcaat
     2941 tatttagatt cactagtaca agcaaatgca gttgatgaaa agttgataga cgatgctgta
     3001 aggcgtgttt taagacttaa atttgattta ggtttattcg acaacccata cactgatttg
     3061 tcgaagaaag attctgtatt gtattcggaa gagcatttaa gggccg
//
LOCUS       DAOPID010000188         3101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_43672_length_3101_cov_3.397571, whole genome shotgun sequence.
ACCESSION   DAOPID010000188 DAOPID010000000
VERSION     DAOPID010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43672_length_3101_cov_3.397571"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..101)
                     /locus_tag="PK641_06135"
     tRNA            complement(29..101)
                     /locus_tag="PK641_06135"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(66..68),aa:Thr,seq:cgt)
     gene            286..483
                     /locus_tag="PK641_06140"
     CDS             286..483
                     /locus_tag="PK641_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HPG55958.1"
                     /translation="MQNVGTNAGLVWQALNGAEGALEVKNLKKVTKLADKDLYASFGW
                     LLREGKISIKEEGKEVFISLV"
     gene            complement(518..1720)
                     /locus_tag="PK641_06145"
     CDS             complement(518..1720)
                     /locus_tag="PK641_06145"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HPG55959.1"
                     /translation="MQKRILLLIIICLSACPIGIYAFPQITDSAKVSILYCSPGEEIY
                     ELFGHTGLRVCDSSKNIDIVFNYGLFDFNQKDFIMRFVRGKTDYQVGVNMYNDFIYIY
                     ESRGSGINECVLDLSPEEKKEIIDYLLWNIKPENCVYRYNFIFNNCATKIRDIIEQSV
                     HGKIIYPSITESISFREAIMQYTKNAAWSQFGFDICLGMGMDRMATERELMFLPENLN
                     LTYAESTVVDSTYNVKNIVSSNEQIIPILREEGKQWFTPTLCGYILLALIIALSVFGY
                     INHKSFKVFDITIFTINGLFGCVILFLILFSQHPFTNDNYNIIWLNPLAFFPLLTLIF
                     KSLKKIKVIYYGFVSIVLTFFLVSMPIIPQFFNDAVVPFTLIYLVRSASHALYWGYHR
                     NTKKGQNN"
     gene            complement(1770..3095)
                     /locus_tag="PK641_06150"
     CDS             complement(1770..3095)
                     /locus_tag="PK641_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPG55960.1"
                     /translation="MGAKKNFVLDTNVILHDYKCIYNFQENDIYLPIVVLEELDKFKK
                     GNEEINYNARQFSRELDQISDNDFFKKGASLGEGLGKLFIVMSQAPSETLKQAFIEKT
                     SDHRILAVTDNLQTSDKKTKTILVTKDINLRMKARALNLLSEDYFNDKISSKDIFEKD
                     QEIFENIDSALIDRMYSTAAGVPIDSFEFKKDIVPNQYFVLKSDRNTIMARYDPNIES
                     VVKVRKERSFGIEPRNSEQSFAMNALLDDNIKLVCITGKAGTGKTLLALAAALEKSHD
                     YKQILLARPIVALANKDIGFLPGTEKDKIAPYMQPLFDNLNVIKHQFSNESREANSIS
                     EMQQSGQLVIEALAFIRGRSLSDAYIIVDEAQNLTPHEIKTIITRAGENTKVVFTGDV
                     QQIDQPYLDMLSNGLVYTIDKMKGQNIFAHVNLIKGERSYLSELASNLL"
BASE COUNT         1036 a          507 c          490 g         1068 t
ORIGIN      
        1 ttcacacaac agattataac attttttgag cctctaaagg gactcgaacc ctcgacctac
       61 gcattacgaa tgcgttgctc taccaactga gctaaagagg cttgaacgcc tgcaaaagta
      121 caataaaaat cgaatgttac aaagaaattt catttaaaaa gaaagtaccg atttttatgt
      181 taaagaagtt aaaaatatat gttttaaaaa catatattat aatataattg ttacttttgc
      241 cgcatgtaac aaggatagtc aattcactat taaatattta gtaaaatgca aaatgtagga
      301 acaaacgctg gtctagtatg gcaagcatta aacggagcag aaggtgctct agaagtaaaa
      361 aacttgaaaa aagtaacaaa attggcagat aaagatttat atgcttcatt cggttggttg
      421 ttacgcgaag gaaaaatttc cattaaggaa gaaggaaaag aagtatttat ttctcttgta
      481 taaataaaaa cacttctatt ataaaaaaag gtcggaatta attattctga cctttttttg
      541 tgtttctgtg atatccccaa tatagtgcat gtgatgctga tcttacaaga tagataagtg
      601 taaatggtac tacagcatca ttaaaaaatt gcggtattat gggcattgaa actaaaaaaa
      661 atgtaaggac tatactcaca aaaccataat aaatcacttt gatttttttt aggcttttga
      721 aaatcaatgt cagcagcggg aagaatgcca aaggatttaa ccaaatgata ttataattgt
      781 cattcgtaaa tggatgctgt gaaaacaata ttaagaatag aataacacat ccgaataatc
      841 catttatagt aaatattgtt atatcaaata ctttaaatga tttatgattg atatatccaa
      901 aaacagataa tgctattatt aaagctaata agatgtagcc gcatagagta ggagtaaacc
      961 attgtttgcc ttcttctcgt aaaataggaa taatttgctc gtttgatgat actatgtttt
     1021 taacattata tgtgctatca actacagttg attctgcgta tgtgagattt aaattctcag
     1081 gtaagaacat aagttctcgt tctgtagcca ttctgtccat acccattcct aagcaaatgt
     1141 caaatccgaa ttgagaccat gctgcatttt ttgtgtattg cataatggct tctctgaagc
     1201 taatagattc tgtaatagaa gggtatatga ttttgccatg aacagattgc tcaattatat
     1261 ctctaatttt tgtagcacaa ttattaaaaa tgaagttata tctgtataca caattttcag
     1321 gctttatatt ccataataaa taatcaatga tttccttctt ttcttcaggt gaaagatcta
     1381 atacacattc gttaataccc gatccacgag attcatatat gtatataaaa tcattataca
     1441 tattgacacc tacttgataa tctgttttac cgcgaacaaa gcgcataatg aagtcttttt
     1501 gattaaaatc gaaaagacca taattgaaaa cgatgtctat atttttagat gaatcacata
     1561 cgcgaagccc tgtgtgtccg aatagttcat atatctcttc gcccggagaa caatatagta
     1621 tggatacttt tgcactatct gttatttgtg gaaaagcata tatgcctatc ggacaagccg
     1681 acaggcatat tataattaac aacagtatcc ttttctgcat aattttcgtt taaaaataca
     1741 cgctgtaaat gtaaatataa atatcgtatt tacaataaat tactagctaa ttcggataaa
     1801 taacttcttt ctccttttat taaattgaca tgtgcaaaaa tgttttgacc tttcatttta
     1861 tcgatggtat ataccaatcc attcgacaac atatccaagt aaggttgatc aatttgttga
     1921 acatcacccg taaaaacaac ctttgtattt tcgcctgcac gtgtaataat tgtttttatt
     1981 tcgtgaggtg ttaaattttg agcttcatct acaattatat aagcatccga caaacttcgt
     2041 cctctgataa aggctaatgc ttcaattaca agttgacccg attgttgcat ttcgctaatt
     2101 gaattagctt cgcgactttc gttcgaaaac tgatgcttaa taacgttcaa attatcgaac
     2161 aatggttgca tataaggtgc tatcttatcc ttttcggttc caggtaaaaa tccgatatct
     2221 ttatttgcca aagctacaat aggtctggca agtaaaatct gcttgtaatc atgtgatttc
     2281 tcgagtgcgg cagctaatgc aagtaaagtc tttcctgtac cggctttacc tgtaatacat
     2341 acaagtttaa tattgtcgtc aagtagtgca ttcatagcaa atgattgctc cgaatttcga
     2401 ggttcaatac cgaatgagcg ttctttgcgt actttaacta cagactcaat atttggatcg
     2461 tacctagcca taattgtatt acgatcactt tttagtacaa aatattgatt aggtacaata
     2521 tcctttttaa attcgaatga gtcgataggc actccggcag cagtgctata cattctatca
     2581 atcaaagcac tgtcaatatt ttcaaatatc tcttgatctt tttcaaatat atctttgctg
     2641 cttattttgt cgttaaaata gtcttcagat agtaaattaa gcgcacgagc cttcatgcgt
     2701 aagtttatat cttttgttac taagatagtt tttgtttttt tgtcacttgt ctgcaagttg
     2761 tcagtaactg caagaattcg atgatctgaa gttttttcaa tgaatgcctg cttaagggtt
     2821 tctgaaggtg cttgtgacat tacaatgaat aatttcccta aaccctcacc aagacttgct
     2881 ccttttttaa agaaatcatt atcagatatt tgatctaatt cgcgagaaaa ttgacgtgca
     2941 ttgtaattga tttcttcgtt accttttttg aatttgtcta attcttctaa aacaacaatt
     3001 ggtaaataaa tgtcgttttc ttgaaagttg tagatacatt tataatcgtg aagaataaca
     3061 ttggtatcca gcacaaagtt ttttttagct cccatagcga t
//
LOCUS       DAOPID010000189         3099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_43703_length_3099_cov_4.514126, whole genome shotgun sequence.
ACCESSION   DAOPID010000189 DAOPID010000000
VERSION     DAOPID010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43703_length_3099_cov_4.514126"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..559)
                     /locus_tag="PK641_06155"
     CDS             complement(104..559)
                     /locus_tag="PK641_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HPG55961.1"
                     /translation="MEKVDELDKEILRIIIKNARIPFAEVAETCGVSRAAIHQRVQKL
                     MDTSVIEGSGYAVNAQKLGYSTCTYIGIRLEKGSMYKTVAPELKKIPEITECHFTTGA
                     YTMLIKLYARNNEHLMELLNDKIQEIHGVVSTETLISLDRSFQREIPIF"
     gene            complement(636..1502)
                     /locus_tag="PK641_06160"
     CDS             complement(636..1502)
                     /locus_tag="PK641_06160"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPG55962.1"
                     /translation="MKKTTILTVLAVAAGLLMTSCNNKSVKVESNLDSISYAAGIVNS
                     YGLTPYLSEKMEIDTTYMDEVIRGIKEASGAKSEKEKAYLAGLQIGSQIQSQIPYMNE
                     SIFSSDTLTKIDENIFYEAFFASLKKDSVMMNVEDASSFIKAERDKRNVAEMEKLYGS
                     NKQAGIAFLKDNKSQEGVVETASGLQYKVVKMGNGAKPLATDRVKVNYHGTLIDGTVF
                     DSSVERGTPAEFGLNQVIQGWTEGLQLMPVGSKFTFYVPYQLGYNDRESGSIKPYSTL
                     IFDVELLEIVKK"
     gene            complement(1529..2230)
                     /locus_tag="PK641_06165"
     CDS             complement(1529..2230)
                     /locus_tag="PK641_06165"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPG55963.1"
                     /translation="MMKKLVFILCAFAFCFNVIQAKKKTVQKPISFELATEADSASYA
                     LGVNMGSQIATNLTKDGYRLDIFAQGFNAALFSAPTKISVDSAEIFLNNYMRKMEEAA
                     TKFKIKEQETFLAENKKREGVKETESGLQYLIVKLGTGEKPTATDKVKVNYEGSLLNG
                     TKFDSSIDRGEPLVMQLNRVISGWQEGVQLMPVGSKFVFYIPYELAYGKQGAGQVIPP
                     YSTLIFEVELLEIVK"
     gene            complement(2227..2814)
                     /locus_tag="PK641_06170"
     CDS             complement(2227..2814)
                     /locus_tag="PK641_06170"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPG55964.1"
                     /translation="MDKVSYALGLNVGNNILSSGIKNLDLANFGQGIKDVMTGENPQM
                     SYEEANDVLNKFFSELAEKINGKNKEIGAAFLAQNKSKEGVIELASGLQYQVLKQGEG
                     IKPAATDRVQCHYEGMLLDGKVFDSSVKRGEPAVFGVNQVIKGWIEALQLMSEGSKWR
                     LFIPSELAYGASGAGQDIPPHATLIFDVELIKVLK"
BASE COUNT         1009 a          591 c          421 g         1078 t
ORIGIN      
        1 gaacgtgggc tcatgaatta tcactgatat atgttattaa accgaaacgc aaagatcttt
       61 gtcctgtggt ttattggtaa acctcagaca aagaattatt gcactaaaat ataggtatct
      121 cgcgctgaaa actgcgatca agtgaaatta aggtctcggt cgaaacaact ccatgtatct
      181 cctgaatttt atcattaaga agttccatta gatgttcatt attacgtgcg tacaacttaa
      241 tcaacatcgt atatgcacct gttgtgaaat ggcattcagt tatttcagga atttttttca
      301 attcaggcgc aacagtttta tacatagaac ctttttcaag acggattcct atatatgtac
      361 atgttgaata tcctaatttt tgagcattaa ctgcatatcc tgaaccttct atcacactgg
      421 tatccatcaa tttctgaact ctttgatgta tagcagcacg cgatacaccg catgtttcag
      481 ccacttctgc gaatggaatt ctagcatttt taattataat gcgcagaatc tctttatcaa
      541 gttcatctac tttttccatt atttaattat attgaagtta aaaaacccgc catataaaaa
      601 tacggcggga ttattataaa attgatttaa ttatcttact tttttacaat ttccaataat
      661 tcaacatcga atattaatgt tgaatatggt ttaatgctgc ctgattcacg atcattgtat
      721 cctaattgat aaggaacata aaatgtaaat ttagagccga caggcattaa ttgcaaacct
      781 tctgtccaac cttgaataac ttgatttaaa ccaaattctg caggagtacc acgttcaact
      841 gagctatcaa atacagtacc atctattaat gtaccatgat aattaacttt aacgcgatcc
      901 gttgcaagag gtttagcacc attacccatt tttacgacct tatattgcaa accactagct
      961 gtttcaacta caccctcttg tgatttatta tccttcaaga aagcgatacc tgcttgtttg
     1021 tttgacccat ataatttttc catttcagca acgttacgtt tgtctctttc tgctttaata
     1081 aatgaagaag cgtcttctac attcatcata actgaatctt tctttaaaga agcaaaaaat
     1141 gcttcataaa aaatattctc atctatttta gtcaatgtat ctgaagaaaa aatagattca
     1201 ttcatataag gaatctgact ttgtatttga gaaccaattt gcaaacctgc tagatatgct
     1261 ttttctttct cggatttagc acctgatgcc tctttaatac cacgtataac ctcatccatg
     1321 tatgtggtat caatctccat tttctctgac aaataaggag tcagaccata tgaattaaca
     1381 atacctgctg cataagaaat tgaatccaaa ttactctcta ctttcactga tttgttattg
     1441 caagatgtca tcaacaaacc tgctgcgaca gctaaaactg tcaaaattgt cgtttttttc
     1501 atgattaaaa taaattaaat tataattgtt attttactat ttctaataat tcaacttcaa
     1561 atattaaagt cgaatatggg gggataactt gtcctgctcc ttgttttccg tatgctagtt
     1621 catatggaat ataaaaaaca aattttgagc cgacaggcat caattgtact ccttcttgcc
     1681 aacctgatat tactctattt agctgcataa ctaatggttc acctctatca atagagctat
     1741 caaatttagt tccatttagt aatgagcctt cgtaattaac tttcacctta tctgtagccg
     1801 taggtttttc tcctgttcct aattttacaa tcaaatattg caaaccgctc tcagtttctt
     1861 taacaccttc gcgcttttta ttttccgcta aaaaagtttc ttgttcttta attttgaatt
     1921 tcgttgcagc ttcttccatt ttacgcatat agttattcaa aaatatttcg gcagaatcaa
     1981 ccgaaatttt tgtaggagct gaaaataatg cagcattaaa accctgagca aatatatcaa
     2041 gtcgataacc atctttagtt aaatttgtag ctatctgact tcccatattt acaccaagtg
     2101 catagcttgc tgaatcagcc tcagttgcca attcaaaaga aataggtttt tgaacagttt
     2161 tctttttcgc ctgaattacg ttaaaacaaa atgcaaaagc gcataaaatg aaaacaagct
     2221 ttttcatcat ttcaatactt taattagttc tacatcaaaa attaacgttg catgaggagg
     2281 aatatcttga cctgcaccgg atgcaccata tgctaattct gaaggtataa ataaacgcca
     2341 ttttgaacct tccgacatca attgtaaagc ttcaatccaa cctttaataa cttgatttac
     2401 accgaataca gcaggttctc cacgtttaac agagctgtca aatacttttc cgtctaacaa
     2461 cattccttca taatgacatt gtacacgatc ggttgctgcc ggtttaatcc cttcaccttg
     2521 tttcaaaact tggtattgca aaccgctagc taactcaata actccttctt ttgacttatt
     2581 ttgagccaaa aaagcagctc ctatttcttt attcttgccg ttgatttttt cggccaactc
     2641 actaaaaaac ttatttaaca cgtcatttgc ttcttcgtaa gacatttgtg gattttctcc
     2701 tgtcattaca tcttttattc cttgaccgaa attagccaaa tctaaatttt taattccact
     2761 gcttaaaata ttgttgccaa catttaagcc taaggcataa cttactttat ccataataaa
     2821 taattgaggt acaaagatac aatatactat tagacaataa aacagaatta ctgcaaaata
     2881 aatataatta tagcatatca tattagtggt taccactata tgtgaaaaaa aaatcatttt
     2941 gtttatatat aatcgtcaat tatttacgtt atataaaaca tgttgttaat aaaaataata
     3001 agaaatcgga catttgttcg aaaaaaggta ttagttttgt gccttgaaaa ttcaaataag
     3061 aagtgcgaaa cgcaaaaagc ataaaagaat tgaatttga
//
LOCUS       DAOPID010000190         3086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_43941_length_3086_cov_2.700099, whole genome shotgun sequence.
ACCESSION   DAOPID010000190 DAOPID010000000
VERSION     DAOPID010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43941_length_3086_cov_2.700099"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="PK641_06175"
     CDS             complement(<1..859)
                     /locus_tag="PK641_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014298623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase"
                     /protein_id="HPG55965.1"
                     /translation="MQKLLLLADEAIALGALDGGLSGVYAYPGTPSTEITEYIQQSKE
                     ASKKNVHCKWSTNEKTAMEAAVGMSFVGKRAMVCMKHVGLNVAADPFMNSAVTGVNGG
                     LIVLSADDPSMHSSQNEQDSRYYGQFAQIPILEPSNQQEAYDMAYEGLELSERLGRPI
                     MLRITTRLAHSRSGVERRPVKAQNELKYPKDPKQFILLPAIARKRYSDLLASQSLFQE
                     SSEKSKFNLLKEGSNKKTGIIACGIAYNYLMENINIAADYPILKICQYPLPRKQVEKI
                     MSDCDSILVI"
     gene            complement(879..2330)
                     /gene="dnaB"
                     /locus_tag="PK641_06180"
     CDS             complement(879..2330)
                     /gene="dnaB"
                     /locus_tag="PK641_06180"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HPG55966.1"
                     /translation="MGKLPPQALELEEAVIGALMLEKDAYFDISEILRPESFYNDHHR
                     YIFEAITQLALQRKPIDMLTVGEQLKVMGKLEEAGSQIYLAELTSKVASATSIVYHSQ
                     IIAQKYLARELIRVATDIQTKAFDEKTDVDDLMQNAEGQLFEVTQRNIKKDVTPVGEI
                     LSEAINRIQLAGQQENGYTGVPSGFNRLDAITNGWQPSTLNIIAARPAMGKTAFVLSM
                     AKNMAVDYQKAVAIFSLEMSNLELVNRLVVNVSEIEGEKIKRGKLVEYEWMQLQSRTT
                     QLVNAPIYIDDTPSLSVFELCSKSRRLKNEHKIDCIIIDYLQLMNASGMSYGSREQEV
                     SIISRSLKALAKELNIPIIALSQLNRGVEGRTGDEKRPQLSDLRESGAIEQDADMVLM
                     IHRPEYYKIMEDRDHNSTEGIAEIIIAKHRSGSVGDVRLRFKKEFIKFTNLDENNDSN
                     YFDTPAGWVPNNDSLYNNPLDNDPLGNNINPLN"
     gene            2540..>3086
                     /gene="ispE"
                     /locus_tag="PK641_06185"
     CDS             2540..>3086
                     /gene="ispE"
                     /locus_tag="PK641_06185"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HPG55967.1"
                     /translation="MITYYPNAKINLGLNIVEKRPDGYHNIETIFYPIPLCDSLSIKS
                     AQKLDFKILGITIDGNIEDNLVMKAYRLIEKDYNIPPISIILEKKIPFGAGLGGGSAD
                     AAYMLRLLSDYFKLSISNDKLEHYASKLGADCPFFIKNEAVFASGIGNIFELSNISLK
                     GYYMMLFKPNISVSTADAYRMV"
BASE COUNT          933 a          560 c          474 g         1119 t
ORIGIN      
        1 ctattaccaa tatactatca caatctgaca ttattttttc aacttgttta cgaggtagtg
       61 gatattgaca tattttcaaa atagggtagt ctgcagcaat atttatattc tccattaaat
      121 aattatatgc tataccacaa gctataatac ctgttttttt attggatcct tcttttagaa
      181 gattaaattt tgatttttca gaactttctt gaaataatga ctgcgaagct aataaatctg
      241 aatatctttt tcttgcaatc gcaggcaata atataaattg tttaggatct tttggatatt
      301 tcaattcatt ttgagcttta acgggacgac gttctactcc tgaacgagaa tgagccaatc
      361 ttgtggttat acgaagcatt ataggacgtc ctaaacgttc tgacaattct aaaccttcgt
      421 atgccatatc gtatgcttct tgttgatttg atggctctaa tattggaatt tgtgcaaatt
      481 gaccataata tcttgaatct tgctcatttt gcgaggagtg catagatgga tcgtcagctg
      541 aaagtacaat taaaccacca ttaacacccg taacagcgct attcataaaa ggatctgcag
      601 ctacattcaa tcctacgtgt ttcatacata ccatagctcg tttacctaca aacgacatgc
      661 caacagcagc ttccatagcc gttttttcgt ttgttgacca tttacaatga acgttttttt
      721 tactagcttc ctttgattgc tgtatgtatt cagttatttc ggtagatggt gtacctggat
      781 aagcatatac accagaaagc cctccatcta aggctcctaa tgctatagct tcatctgcta
      841 ataaaagtaa tttttgcata tttattagat atttttttct agtttaaagg attaatatta
      901 ttgcctagag ggtcattgtc taagggatta ttgtataggg aatcattatt tggaacccat
      961 cctgcaggtg tatcaaaata gttgctatcg ttgttttcat caagatttgt aaatttgatg
     1021 aattcttttt taaacctcaa gcgcacatca ccaacagaac cactacgatg tttagcaatt
     1081 atgatttctg ctattccctc tgtagaattg tgatctctat cttccattat tttataatat
     1141 tcaggacgat gtatcattaa taccatatct gcatcttgtt caatagcacc agattcacgt
     1201 aaatctgata attgtggacg tttctcatca cctgttcttc cttcaacgcc acggtttaat
     1261 tgtgatagtg cgataatcgg tatgtttaat tcttttgcca aggctttgag tgacctggat
     1321 atgatactta cttcttgttc acggcttcca taactcatac cgcttgcatt catcaattgt
     1381 aagtagtcaa ttattataca atctatttta tgctcatttt ttaagcgtct tgatttgctg
     1441 cataattcaa aaacagaaag gctaggggta tcatctatat aaataggtgc attaaccaac
     1501 tgtgtagtac gagattgtaa ttgcatccac tcatactcaa ccaattttcc tctttttatc
     1561 ttttcgcctt cgatttcaga tacatttaca accaaacgat tgaccaattc gaggtttgac
     1621 atttctaatg agaatatggc aacagctttt tgatagtcaa cagccatatt tttcgccata
     1681 gataatacaa atgccgtttt acccatagca ggacgagctg caataatatt taaagtagat
     1741 ggttgccaac catttgtaat agcatccaat cgattaaaac ctgaaggaac acctgtatat
     1801 ccgttttctt gttgtccggc taattgaatt ctattaattg cttcagaaag tatttctcca
     1861 acaggagtaa catctttttt tatgtttcgt tgagtaactt caaataattg tccttcagca
     1921 ttttgcatca aatcatctac atctgttttt tcatcaaaag ccttagtttg aatatctgta
     1981 gctactcgta taagttcgcg tgctaaatat ttttgtgcaa taatttgtga atgatacaca
     2041 atacttgttg cagaggccac tttactagtt agttcagcta aataaatctg actcccggct
     2101 tcttccagtt tacccattac ttttagttgt tctccaacag taagcatatc tataggttta
     2161 cgttgtaaag ctaattgtgt tatggcttca aatatatagc gatgatggtc attataaaaa
     2221 ctttcaggac gtaatatctc gcttatgtca aaatatgcat ctttttctag cattaatgca
     2281 ccaattacag cctcctctaa ttcaagcgct tgtggtggta atttccccat ttctttaata
     2341 accttatccg aaacagtacc gtaagctgta gtagtttttt ttctttttac ttgagaagat
     2401 tccatacttt tattttatag cccaaaagta ctaaatttac acttagaact aacattaaaa
     2461 acagagtttt acttaacaat ttatcgacaa ccttatgttg atatgtatta ttttgtaatt
     2521 ttgtggaaac ttcacatata tgattacata ttatccaaac gcaaaaatca atctaggact
     2581 taatatagta gagaaacgtc ctgacggata tcataacata gaaacaatat tttacccaat
     2641 tccattatgt gacagtttat caataaaatc agctcaaaaa cttgatttta agatacttgg
     2701 cattacaatt gatggcaata ttgaagataa tctggtaatg aaagcttatc gtcttattga
     2761 aaaagactac aacatacctc cgatatctat tatacttgag aaaaagattc cttttggagc
     2821 cggtttaggt ggtggttctg ctgatgcagc atacatgctc cgcttattat ctgactattt
     2881 taaattgtca atatctaatg ataaactcga acattatgca tcaaaattag gtgcagattg
     2941 tccgttcttt attaaaaacg aagctgtttt tgcatccgga ataggtaata tttttgaatt
     3001 atcaaacatc tcattaaaag gatattacat gatgttgttt aaacctaata tttctgtttc
     3061 aactgctgat gcatatcgaa tggttg
//
LOCUS       DAOPID010000191         3045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_44783_length_3045_cov_5.151839, whole genome shotgun sequence.
ACCESSION   DAOPID010000191 DAOPID010000000
VERSION     DAOPID010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44783_length_3045_cov_5.151839"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="PK641_06190"
     CDS             complement(<1..859)
                     /locus_tag="PK641_06190"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPG55968.1"
                     /translation="MKDKINSLLQEIKNLSPTSKEDVEQLRIKYLSKKGEISLLFNDF
                     KNVAGEEKRIMGQMLNQLKDIAQNKFNEMKEVYENAASKNDEIDLTRTASAIELGTRH
                     PLSIVRNEIIDIFSRIGFIIAEGPEIEDDWHVFGALNFPPEHPARDMQDTFFIEKNPD
                     VLLRTHTSSVQSHIMTTQKPPIRVLCPGRVYRNEAVSYRAHCFFHQVEGLYIDKNVSF
                     ADLKQALLYFAKEMFGEDTQIRLRPSYFPFTEPSAEMDISCDLCGGKGCNFCKHTGWV
                     EILGCGMVDP"
     gene            1008..1778
                     /locus_tag="PK641_06195"
     CDS             1008..1778
                     /locus_tag="PK641_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPG55969.1"
                     /translation="MGKIIALANQKGGVGKTTTTVNLGASLAVLEKKVLIVDADPQAN
                     ASSGLNIDVTTLENSIYECLINGVDVKKAIVKTEVEGLDLLPSHIDLVGAEIEMLKLE
                     NREKFLKNMLTPLKEEYDYILIDCSPSLGLITVNVLTAADSVIIPVQAEYYALEGISK
                     LLNTIKIIKSRLNPELEIEGFLLTMYDSRLRLANQVYTEVKQHFQNMVFDTIIQRNIK
                     LSESPSHGVPVLLYDADSKGAQNHMQLAQELINKNGKK"
     gene            1779..2660
                     /locus_tag="PK641_06200"
     CDS             1779..2660
                     /locus_tag="PK641_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004345513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPG55970.1"
                     /translation="MNSNNKKQALGRGLGALINNDIVETKGSSSISEVELSLIEANPN
                     QPRTHFDEEALSELSASIKEIGVVQPITLREMPSGKYQIIAGERRCRASKMASLERIP
                     AYIKTVDDESMMEMALVENIQREDLNAIEIALSYQRLIDEYQFTQDKLSDRVGKKRAT
                     ISNYLRLLKLPAEIQLGIKYKKIDMGHARALVSIDDTAEMIRLYEDIIKHAYSVRKVE
                     EMAHNISNKTENKTAKKQVNDMSDYISLCGKLSKLFGTAVNIERNSKGKGRICLQFST
                     DDELENLIATFDKMRDA"
     gene            2653..>3045
                     /locus_tag="PK641_06205"
     CDS             2653..>3045
                     /locus_tag="PK641_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HPG55971.1"
                     /translation="MRNTLRYIQFLIIFLLPLQVFAGEVLLSDSAKLAVTDSIFVEKK
                     LQELPDSTYSVVKKSFKPDADKAVWFAAVVPGLGQIYNRQYWKLPIIYGGAMGLAYAI
                     SWNNQMYVDYRKGYVDIIDGDPDSRAYES"
BASE COUNT          998 a          485 c          588 g          974 t
ORIGIN      
        1 taggatctac cataccgcaa cctaaaattt ctacccaacc ggtatgttta cagaaattgc
       61 atcctttccc gccgcataaa tcacaagata tatccatttc agcacttggt tcagtgaatg
      121 ggaaatatga agggcgtaat cttatttgag tgtcttcacc aaacatttct tttgcaaaat
      181 atagcaatgc ttgttttaaa tcagcaaaag aaacattttt gtcaatatac aaaccttcta
      241 cctgatggaa aaagcaatgc gctctatatg aaacagcctc atttcgatag acacgtcccg
      301 gacatagtac tctgatagga ggtttctgtg ttgtcataat atgagattga acactcgatg
      361 tatgtgtacg caataaaaca tcaggattct tttctataaa gaatgtgtct tgcatatcgc
      421 gtgcaggatg ctccggtgga aaattcaaag ctccaaatac atgccaatcg tcttctattt
      481 cgggaccttc tgctatgata aatccaatac gcgaaaatat atctatgatt tcgtttctaa
      541 caattgacaa tggatgtctt gttcctaatt caatagcaga tgcagtacga gtcaaatcaa
      601 tttcgtcgtt tttcgaggca gcattttcat aaacctcttt catttcattg aatttgtttt
      661 gtgctatatc ttttaactga ttgagcattt gacccattat gcgtttttct tcgccggcta
      721 cgtttttgaa atcattaaac aacaaagata tttcaccttt tttgctaaga tattttatac
      781 gtagttgttc tacatcttct ttacttgtcg gtgacaaatt ttttatttcc tgtaataggc
      841 tgtttatctt atccttcatg ctttaaaatt ttctcacggt tgcaaaaata cgacttttat
      901 ccgagaataa aggaatttta tattactttt acatgtgcat cattcctttt ttttttactt
      961 ttgcagttta gatttatgtg gaaaggaata aagtaaagat tatttatatg ggaaaaatta
     1021 ttgcattggc aaatcaaaaa ggcggcgtag gaaaaactac gactacggtc aatcttggcg
     1081 catccttagc tgttcttgag aagaaagtat taatagttga tgcagatcct caagcaaatg
     1141 cttcatccgg tctgaacatt gacgtcacga cattagaaaa tagtatttat gagtgtttga
     1201 taaatggggt tgatgtcaaa aaggcaatag taaaaacaga ggtcgaaggc ttggatttat
     1261 taccatctca tatagatttg gttggagcag aaatagagat gctaaagttg gaaaacaggg
     1321 aaaagttttt gaaaaatatg ttgacaccgc ttaaagagga gtatgattat attttgattg
     1381 attgttcgcc atctttaggt ttaataactg taaatgtatt aaccgctgct gattctgtaa
     1441 ttattcctgt acaagcagaa tactatgcac tagagggtat tagtaaatta cttaatacta
     1501 ttaaaataat taaaagtcgc ttgaatcctg aattggaaat agaaggcttt ttgttgacaa
     1561 tgtatgattc acgtttacgc ttggcaaatc aagtatatac agaggttaaa caacatttcc
     1621 agaatatggt tttcgatacg attattcaac gtaacataaa acttagtgaa tctcctagtc
     1681 atggcgttcc tgttttgtta tatgatgccg attcaaaagg agctcagaat cacatgcaat
     1741 tggctcagga attgattaat aaaaacggaa agaaataaat gaacagcaat aataagaaac
     1801 aagccttagg gcgtggttta ggtgctttaa tcaataatga tattgtagaa acaaaaggtt
     1861 cttcttctat aagtgaggtc gaattgtcat tgattgaagc gaatcctaat cagccgcgaa
     1921 cacattttga cgaagaagct ttgtctgagt tatcagcatc aataaaggaa ataggtgtcg
     1981 ttcaacctat cactttgcgt gaaatgcctt caggaaagta tcaaattatt gcaggtgaaa
     2041 gacgttgcag agcttcaaaa atggcaagct tagagcgcat acctgcatat attaaaactg
     2101 ttgatgatga gtcaatgatg gaaatggctt tggttgaaaa tattcaacgt gaggatttga
     2161 acgccatcga aattgcattg agttatcaac gtttgataga tgaatatcaa ttcactcagg
     2221 ataaactaag tgatagggta ggaaagaagc gtgctactat ttcaaattat ctacgtttac
     2281 taaaattgcc ggcagaaata caacttggta taaaatataa aaaaattgat atggggcacg
     2341 caagagcttt agtgtcgatt gacgatactg ccgagatgat acgtttgtat gaagatatta
     2401 ttaagcatgc ctattctgtg cgcaaagttg aagaaatggc gcataatata tcaaataaga
     2461 cagaaaataa aactgctaaa aagcaagtta atgatatgtc cgactatatt tcattgtgcg
     2521 gcaaactttc caaacttttc ggaactgcgg ttaatataga aaggaattca aaaggcaaag
     2581 gcagaatatg cttacagttt tctacagatg atgaattgga aaatttgatt gctacattcg
     2641 ataaaatgag agatgcgtaa tactttacgc tacatacaat tccttattat tttcctcctg
     2701 ccattgcaag tctttgcagg agaggttctt ctttctgatt cagcaaagtt agctgtaacc
     2761 gacagcattt ttgttgagaa gaaattgcaa gaactacctg attctactta tagtgtagtt
     2821 aaaaagtcgt ttaaaccgga cgctgataag gctgtatggt ttgcagcagt agttccgggt
     2881 ttaggccaaa tatataacag gcagtattgg aaactaccta ttatttatgg tggagctatg
     2941 ggcttagctt atgctataag ttggaataat cagatgtatg tagattacag aaaaggatat
     3001 gttgacatta ttgatggaga tcccgattcg agagcatacg aaagc
//
LOCUS       DAOPID010000192         3011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_45495_length_3011_cov_4.476319, whole genome shotgun sequence.
ACCESSION   DAOPID010000192 DAOPID010000000
VERSION     DAOPID010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45495_length_3011_cov_4.476319"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1657
                     /locus_tag="PK641_06210"
     CDS             <1..1657
                     /locus_tag="PK641_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPG55972.1"
                     /translation="PFEYLNEVLNIKKGDVYNQKKISQRLFEDDDCVSNLYLDNGYLF
                     FNMQPVETNISNDSIDMEIRMAEGRQATINRVNIQGSEYLYEHVVRRELRTKPGDLFR
                     KSDIMRSMRELAATGHFSTDPGKMDIKTVPDPENGTVDLTYVLESKRNDQIELSAGWG
                     QTGVVGTLKLKFTNFSLRNIFNFKSYRPLPQGDGQTLTLSAQTNGMYYQAYSLSFIEP
                     WLGGKRPNSFSFSAYYSIQSGVSNSYSSSYNNGYYYNNNSYNTWYSSSYDPDKYIKIL
                     GISAGIGTRLNWPDDYFTIYGQISYQNYNLSKWDYFALNTGVANNLNISAVLARNSLD
                     QPIYTTRGSEISLSVALTPPYSLWDNIDYSKASQQDIYRWIEYYKIKFKSRFFLPLTK
                     NNKLVLMGRAELGFLGYYNINKRSPFETFWVGGDGMTGYSSTYATETVALRGYTNGSL
                     TPYYNGYQAGNIYARFSIELRYPIVLKESTMVWALVFAEAGNCWRDYNNFNPFDLKRS
                     AGVGLRVYMPMIGLLGVDWAYGFDKVNGTVSGSQFHFVIGQEF"
     gene            1709..2221
                     /locus_tag="PK641_06215"
     CDS             1709..2221
                     /locus_tag="PK641_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPG55973.1"
                     /translation="MKRLALLTVCALCVTGSTLAQKFAFVDTEYLMKNIPLYESATQQ
                     LEQSSKKWQKEVDALNTEAQTMYKDYQSNMVYLSDEQKKEQEQAIVAKETEAKELKRK
                     YFGSDGELFKKKESLMKPIYDELYNALKDLSEGKGYTAIWDRATGMILFVNPSIDISD
                     DVLTKMGYAK"
     gene            2266..2772
                     /locus_tag="PK641_06220"
     CDS             2266..2772
                     /locus_tag="PK641_06220"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPG55974.1"
                     /translation="MKKLIIALFIVLPLGVFAQQDLKIGYINKQELITSMSEYTTAMK
                     QLEDMNLKYITEGKKLQEEFQKKYQEYAETQDSLDATIKKYKESELMRLQQSIEEFSK
                     SAEENLKKKQQELVAPILEKMDKAIKQVGDENGFTYIIDNSAGIISYTSAKSINVLPL
                     AKKALNIQ"
     gene            2783..>3011
                     /locus_tag="PK641_06225"
     CDS             2783..>3011
                     /locus_tag="PK641_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HPG55975.1"
                     /translation="MSSNKSIGIFDSGYGGLTILEEIRALLPEYDYIYLGDNARNPYG
                     TRSFEVVYEYTLQCVKKLFEMDCKLIILACNT"
BASE COUNT         1087 a          496 c          541 g          887 t
ORIGIN      
        1 tccttttgaa taccttaatg aagtgttgaa catcaaaaaa ggagatgttt ataatcagaa
       61 aaaaatttct caacgcttat ttgaagatga tgattgtgta agcaatttat atttggataa
      121 cggatatcta ttctttaaca tgcaacctgt cgaaactaat attagcaacg attctattga
      181 catggaaata aggatggcag aaggtagaca agcaacaatc aatcgagtta atatacaagg
      241 tagcgaatat ctatatgagc atgtagttag acgtgaactt agaacaaaac ccggtgattt
      301 gtttagaaaa tctgatatta tgcgttctat gcgtgaactt gccgcaacag gacatttctc
      361 aacagacccg ggtaagatgg atattaaaac agtccctgac cctgaaaacg gcactgttga
      421 tttaacctat gtactcgaat caaaacgtaa tgaccaaatt gaattatctg caggttgggg
      481 acaaacaggt gttgtaggaa ctcttaaatt aaagttcacc aatttctcgc tgcgtaatat
      541 atttaatttc aaaagttatc gtcctctacc acaaggtgat ggacaaactt taaccctgag
      601 tgcacaaaca aatggtatgt actatcaagc atatagttta tcgtttattg agccgtggct
      661 tggtggcaaa cgtcctaaca gtttttcttt ctctgcatac tattcaatac aatcaggtgt
      721 aagcaatagc tacagctcat cttataacaa cggatactat tataacaata actcatataa
      781 cacttggtat tcatcatctt acgaccctga taaatacata aaaatccttg gtatttctgc
      841 cggtatagga acaagactta attggccgga tgactatttt actatatatg gccaaatttc
      901 gtaccaaaat tataatttaa gtaaatggga ctattttgca ttaaatacgg gtgtcgcaaa
      961 caacttgaat atctctgctg ttttagctag aaactcattg gatcaaccaa tctacacaac
     1021 acgtgggtca gaaatatcac tatcagtagc tttaactcca ccctattctc tttgggataa
     1081 tattgactac agcaaagcaa gtcaacaaga tatttatcgc tggattgagt attacaagat
     1141 taaatttaag tcaagattct ttttacctct aactaagaac aataagttag ttcttatggg
     1201 acgagcggaa ttaggatttt taggatatta caatataaac aaacgctctc cgtttgaaac
     1261 tttctgggtt ggtggtgatg gtatgacagg atacagcagt acatacgcaa ctgaaacagt
     1321 agctttacgt ggatatacaa atggttcact tacaccatat tacaatggat atcaggctgg
     1381 taatatatat gctcgtttct ccattgaatt gagatatcct atcgtactaa aagagagtac
     1441 tatggtttgg gctttagtat ttgctgaagc aggtaattgc tggcgcgatt ataacaattt
     1501 caatccattc gacttaaaaa gatcagccgg tgttggtcta cgtgtatata tgccaatgat
     1561 cgggctgtta ggcgttgatt gggcttacgg atttgacaaa gtgaatggta ctgtcagtgg
     1621 aagtcaattc cacttcgtaa taggacaaga gttttaaact ttttctctat atttgcatct
     1681 aattcaacgt gaacataaaa ttatagatat gaaaagatta gcattattaa cagtatgcgc
     1741 actttgcgta acaggttcaa cgttagcaca aaaattcgca ttcgttgata ctgaatattt
     1801 aatgaaaaat attcctttgt acgaatcagc gacccaacaa ctggaacaaa gttctaagaa
     1861 atggcaaaaa gaagttgatg ctcttaatac agaggcacaa acaatgtaca aagactatca
     1921 atcgaacatg gtttatctta gtgatgagca aaaaaaagaa caagaacaag ctattgtagc
     1981 caaagaaaca gaagcaaaag aattgaaacg taaatatttc ggttctgatg gcgaattatt
     2041 caagaaaaaa gagagtttga tgaaacccat ttatgacgaa ttatacaatg cgcttaaaga
     2101 cctatctgaa ggcaaaggct acactgcaat ttgggataga gccaccggca tgattctttt
     2161 tgtaaatcca agcattgaca taagcgatga cgttttaaca aaaatgggtt atgcaaaata
     2221 actgattagc aattaattaa tattcaatta aatattttta taaacatgaa aaaacttatt
     2281 atcgccctat ttattgtact acctttaggt gtatttgcac aacaagattt aaaaataggc
     2341 tacatcaaca aacaagaatt aatcacatcc atgtctgaat ataccactgc aatgaagcaa
     2401 ttggaagaca tgaacttaaa gtacattaca gaaggtaaaa aattacaaga agaatttcaa
     2461 aagaagtatc aggaatatgc agaaacacaa gattctttgg atgctactat caagaaatac
     2521 aaagaaagtg aattgatgcg tttacaacaa agcattgaag aattttctaa aagcgctgaa
     2581 gaaaacctaa aaaagaaaca acaagaattg gtagctccca ttttagaaaa gatggataaa
     2641 gcaatcaaac aagttggcga cgaaaatgga tttacatata ttattgacaa ctctgcaggt
     2701 attatttcat atacatctgc aaaaagcatt aatgtacttc cacttgcaaa gaaggcttta
     2761 aatattcaat aacataaggt ctatgtcctc caacaaaagc attggcatat ttgactcagg
     2821 ctacggtgga ctgacgattt tagaagaaat tcgggcatta cttcctgagt atgattatat
     2881 atacttaggg gataatgccc gaaatcctta tggaacacgt tcatttgaag ttgtatacga
     2941 atacacacta caatgcgtaa agaaattatt tgagatggat tgcaagttga ttattttagc
     3001 atgtaataca g
//
LOCUS       DAOPID010000193         3008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_45561_length_3008_cov_3.675584, whole genome shotgun sequence.
ACCESSION   DAOPID010000193 DAOPID010000000
VERSION     DAOPID010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45561_length_3008_cov_3.675584"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="PK641_06230"
     CDS             <1..800
                     /locus_tag="PK641_06230"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993399.1"
                     /note="converts 2-oxoglutarate to glutamate; in
                     Escherichia coli this enzyme plays a role in glutamate
                     synthesis when the cell is under energy restriction; uses
                     NADPH; forms a homohexamer; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HPG55976.1"
                     /translation="RKLQHEFTGTFTGKGLEFGGSLIRPEATGYGNIYFLLSMLKTRN
                     IELKGKKCLVSGSGNVAQYTCEKLIELGAIPLTLSDSNGYIYDPNGITTEKLEFIKDL
                     KNVQRGRIKEYADKYGCKYVEGARPWGEKADIALPSATQNEINAEDAQKLVNNGIIAV
                     SEGANMPTTPEAIDIFQKAKLLYAPGKAANAGGVATSGLEMSQNSERLSWSREEVDAK
                     LKGIMADIHANCVKYGTEPDGYINYAKGANIAGFMKVAKAMMAQGII"
     gene            955..1779
                     /locus_tag="PK641_06235"
     CDS             955..1779
                     /locus_tag="PK641_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HPG55977.1"
                     /translation="MNKIISKENFSENVVSLDIEAPLIAKARRAGHFVIVRIDKDGER
                     IPLTIAGSDIEKGTIRLVVQKIGVSSTKLCSLNVGDYIEDVVGPLGKATHIEKFGTVV
                     CACGGVGTAPMLPIVEALKKAGNKVIVVLAARTKDLIILEEQMRQYADEIIIMTDDGS
                     YGTKGLVTNGVENVINREKVDLCVTIGPAIMMKFVSLLTKKYNVPTVASLNTIMVDGT
                     GMCGACRVSIGGETKFVCVDGPEFDAHQVNFDEMMMRLGAYREQEQAAFAKYKEQL"
     gene            1796..>3008
                     /locus_tag="PK641_06240"
     CDS             1796..>3008
                     /locus_tag="PK641_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPG55978.1"
                     /translation="MELTNRTAAWREELRKAMKNKERTEITRVNMNELDGEYRSRNYE
                     EVNQGLNKDQALLEAKRCLDCLNPSCITGCPVNIDIPSFIKNIERGAFLEAAQVLKMT
                     SALPAVCGRVCPQERQCESQCIHLKMGKKAVAIGYLERFAADYERESGSISIPEIAAK
                     NGIKIGVVGSGPAGLSFAGDMAKKGYDVTVFEALHEIGGVLKYGIPEFRLPNSIVDVE
                     IGNLEKMGVKFIKDCIVGKTLSFDDLKEQGFKGLFVASGAGLPNFMGIKGENLIGIMS
                     SNEYLTRINLMGAAQDQFDTPVLKGKRVAVIGGGNTAMDSVRTAKRLGAELAMIVYRR
                     SEEEMPARIEEVKHAKEEGIEFRTLHNPIEYYGDEKGRVKQMLLQKMELGEADASGRR
                     KPVEIEGATELI"
BASE COUNT         1025 a          482 c          661 g          840 t
ORIGIN      
        1 atcgcaaatt acaacacgaa tttacaggta catttaccgg taaaggtttg gaattcggtg
       61 gatcattaat tcgtcctgaa gcaaccggtt atggtaacat ctacttcttg ctaagtatgt
      121 tgaaaacacg taacatcgaa cttaaaggca aaaaatgtct tgtatcaggt tcaggtaacg
      181 ttgctcaata cacatgcgag aaattaatag aattaggagc aattcctttg actctttctg
      241 attctaatgg ttatatatat gatccaaatg gtatcacaac agaaaaattg gaattcataa
      301 aagatctgaa gaacgttcaa cgtggacgta ttaaagaata tgctgataaa tatggttgca
      361 aatatgtaga aggcgctcgt ccttggggag agaaagctga tatcgcattg ccatcagcaa
      421 cacaaaacga aatcaatgct gaagacgctc aaaaactcgt taataacgga atcatagcag
      481 tatctgaagg tgccaacatg cctacaactc ctgaggctat tgatatcttc caaaaagcta
      541 aattattata tgctccgggt aaagctgcaa acgcaggtgg tgttgcaaca tcaggtttgg
      601 aaatgagtca aaactctgaa cgtttaagtt ggtctcgcga agaagttgat gcaaaattaa
      661 aaggtatcat ggctgacatt catgcaaact gtgttaaata tggcactgaa cctgacggat
      721 atatcaacta tgctaagggt gcaaatatcg caggctttat gaaagttgct aaagctatga
      781 tggctcaagg tatcatctaa gcactaacat taactattaa atgcacggat agaaacgggc
      841 gggttaaatt aacccgccca tatccgtttt tataaattga tggttctgaa tattgtattt
      901 atctaaaaat ttaaaaataa cacatatttt gaacttataa ttcaaaaaaa attcatgaat
      961 aagattatat caaaagaaaa cttttcggaa aatgtggtta gcttagacat tgaagcccca
     1021 cttatagcta aagctcggcg agcagggcat tttgttattg ttcgtattga caaagatggc
     1081 gaaagaattc ctttgacaat tgcaggatct gatattgaaa aaggcacaat cagattggtt
     1141 gtacaaaaaa ttggagtttc atcaacaaaa ctatgctcgc taaatgtagg cgactatatt
     1201 gaagatgtag ttggtccact tgggaaagca acacacatcg agaaattcgg tactgttgtt
     1261 tgcgcatgcg gtggtgttgg aacagctccg atgttgccta ttgttgaagc tttaaaaaaa
     1321 gcaggcaaca aagtaattgt agtacttgct gcaagaacta aagatttaat aatcttggaa
     1381 gaacaaatgc gtcaatacgc agacgaaatc attattatga cagatgatgg ttcttatgga
     1441 acaaaaggac ttgttactaa tggcgtcgag aatgtaatta accgcgaaaa agttgatttg
     1501 tgcgtaacta taggtcctgc tataatgatg aaattcgtat ctcttctaac taaaaaatat
     1561 aatgtaccaa cagtagcttc gttaaataca attatggttg atggtacggg aatgtgtggt
     1621 gcatgtcgtg taagcattgg aggcgaaacg aaatttgtat gtgttgacgg cccggaattt
     1681 gatgctcacc aagttaactt cgatgaaatg atgatgcgtt taggagctta tagagaacaa
     1741 gaacaagcag cttttgctaa atataaagaa caattataac acggagaata ttagcatgga
     1801 actaacaaat agaactgctg cttggcgtga agaattgcgc aaagcaatga aaaataagga
     1861 acgtacggag attacccgtg ttaacatgaa tgaattagat ggtgaatacc gtagtcgtaa
     1921 ttatgaggaa gtaaaccaag gattaaacaa agaccaagca ttgcttgaag caaaacgctg
     1981 cttagattgt cttaatccaa gttgtattac aggttgtcct gtaaacatcg acattccgtc
     2041 atttatcaaa aatattgaac gtggcgcatt tttagaggct gcacaggttt taaaaatgac
     2101 atctgcacta cctgcggtgt gcggtcgtgt ttgcccacaa gaaagacaat gcgaaagtca
     2161 atgtattcat ttaaaaatgg gtaaaaaggc tgttgccatt ggttatttgg aacgttttgc
     2221 cgctgactac gaacgcgaaa gcggatctat ttcaataccc gaaattgctg ccaaaaatgg
     2281 cattaaaata ggtgttgtag gttcaggtcc tgcaggatta tcgttcgccg gagatatggc
     2341 aaaaaaagga tatgacgtaa ctgtatttga agcattacac gagattggtg gtgtgttaaa
     2401 atatggtatt cccgaattcc gtttaccgaa ttctattgtt gatgttgaaa taggaaatct
     2461 agaaaaaatg ggcgttaaat ttatcaagga ttgcatcgtt ggtaaaactc tttctttcga
     2521 cgatttaaaa gaacaaggat ttaagggatt atttgttgca agtggagcgg gcctacctaa
     2581 tttcatgggt attaagggag agaaccttat cggtatcatg tcatcaaacg aatatctaac
     2641 acgtattaat cttatggggg ctgcacaaga tcaatttgac actcctgtat taaaagggaa
     2701 acgtgtagca gttattggtg gtggtaatac cgccatggat tctgttcgta cagcaaaacg
     2761 tttgggcgct gaattggcaa tgattgtata tcgtcgcagc gaagaagaaa tgcctgcacg
     2821 tatcgaggaa gtaaaacatg cgaaagaaga aggtattgag tttagaacac ttcacaaccc
     2881 tattgaatat tacggagacg agaaaggtcg tgttaaacaa atgctcttgc aaaaaatgga
     2941 attaggcgaa gctgatgcat cgggacgtcg taaacctgta gaaattgaag gtgcaacaga
     3001 acttattg
//
LOCUS       DAOPID010000194         2977 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_46244_length_2977_cov_4.488706, whole genome shotgun sequence.
ACCESSION   DAOPID010000194 DAOPID010000000
VERSION     DAOPID010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46244_length_2977_cov_4.488706"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..1192
                     /locus_tag="PK641_06245"
     CDS             203..1192
                     /locus_tag="PK641_06245"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPG55979.1"
                     /translation="MTSLKSDNTHYQFNKRTLKYERVEHTWKYHFKRAISVLSLGLIF
                     GAAFFLLFIFTIQSPNEKTLAAENERIGTQFELLSHQLDDAIKILNIIRERDDNFYRV
                     MLEADSIPTTLRTGNYDATARYSQWDDLRTASIVKESSMKMDILNRMLYVQSNSFDEL
                     ISLAKQSEDKLLHVPAIQPVLNKDLKRTASGYGRRIDPIYKTLRFHEGMDFAAPIGTE
                     IYATGNGTIVSVGWKQGYGNCIVIDHGYGYSTVYAHISKFQKSIRKGTKVVRGDVIAY
                     VGNTGKSTGPHLHYEVRFKGKAQNPQNYYFLDLSPAEYDEMVRISANNAKTFD"
     gene            1192..1530
                     /locus_tag="PK641_06250"
     CDS             1192..1530
                     /locus_tag="PK641_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPG55980.1"
                     /translation="MLKEGKLYYSISEVAEEFDLTPSLLRYWEKNFSIIKPKTNDRGT
                     RHYTQKDIQNIRMIKYLLKEKRMTIKGAQKQLQKNNANNVNTTSVIDKLNIIKARLHD
                     IKNEMKYLEE"
     gene            1542..2552
                     /locus_tag="PK641_06255"
     CDS             1542..2552
                     /locus_tag="PK641_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HPG55981.1"
                     /translation="MKRYNFLLVAFMLTMPIMAVNRPNNVIVIIGDGMGLNHICTLNS
                     PNNFERAQAIGLSKTYSANSPVTDSAAGGTAIACGVKTNNGMLGVSQDSIPVKSILTI
                     LKENDLSTGIVVSCRINHATPAAFYAHQPNRGMDSAIVSDLYKSNIDVIVGGGAKFVS
                     TDSLKNLGYEVCNNLDDLKSVKKVGKIACLLAKEDMPVAEQRKDTIPQAVQQALRLLS
                     QNKKGFFLMIEASQIDWASHGNNAKYLKTEIQDINAVLGACMDFADANPGTLIIVTAD
                     HETGGVSLLENEKEYKFSTGKHTGVMVPIYSYGTGAENFSKIQENTGFKNKILDLMHI
                     SK"
     gene            2650..>2977
                     /gene="rplS"
                     /locus_tag="PK641_06260"
     CDS             2650..>2977
                     /gene="rplS"
                     /locus_tag="PK641_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPG55982.1"
                     /translation="MDLIKIAEESLATGKEKTLPSFKSGDTVTISYKIVEGNKERIQQ
                     YRGVVIKIAGHGDKKRFTVRKMSEGIGVERIFPLDSPFIEKLEVNKIGKVRRAKLYYL
                     RDLTGKK"
BASE COUNT         1116 a          460 c          534 g          867 t
ORIGIN      
        1 acttacgtat ttctttagaa atcatcatta tactatatga tttttttgac ttgcaaaaat
       61 agttaatata attggaaatt ttaccaaggt atgtataaaa ataagggcaa gtctttattt
      121 ttgcaaaata gaacaaaaac ttgactgcat atttacaaat agttgagatt taattgtaaa
      181 tttgcgactc aaaaaagtta taatgacctc attaaaatca gataatacac actaccaatt
      241 taacaaacgt acactcaagt atgaacgtgt tgaacacact tggaagtatc attttaaaag
      301 ggctatcagc gttttaagtt taggacttat atttggagct gcattctttt tattattcat
      361 tttcacaatc caatctccta acgaaaaaac tttagcagct gaaaatgaac gtattggaac
      421 tcaatttgaa ttattatcac atcaacttga tgatgctatt aaaattttaa atattattcg
      481 agaaagagat gataattttt acagagtaat gttagaggct gattcaattc ctactactct
      541 tcgtaccggt aattacgatg caacagctcg ttacagccaa tgggatgatt tgcgtaccgc
      601 atctattgta aaagaatcgt caatgaaaat ggacattctt aatcgtatgc tttatgttca
      661 atcaaattct tttgacgaat taattagttt ggccaaacaa agtgaagata aattattgca
      721 tgtacccgct atacaacctg ttttaaacaa agatttaaaa cgtacagcat ccggttatgg
      781 cagacgtata gacccaatat acaagacttt aagatttcac gaaggaatgg actttgctgc
      841 accaataggc acagaaatat acgcaacagg taatggtacg attgtatctg taggttggaa
      901 acaaggatat ggtaattgca ttgttattga ccacggatat ggttattcaa cagtatatgc
      961 acatataagt aaatttcaaa aatcaattcg taaaggcact aaagtggtac gaggagatgt
     1021 gattgcatat gttgggaata ccggaaaatc aacaggcccc catcttcatt acgaagtaag
     1081 atttaaaggt aaggcacaaa atccacaaaa ctattatttc ttggatttgt cgcctgcaga
     1141 atatgatgaa atggtacgta tttctgcaaa taatgctaaa acatttgatt gatgctaaaa
     1201 gaaggaaaat tatattattc aataagcgaa gttgccgaag agtttgactt aacaccatcg
     1261 ttattaagat attgggaaaa gaatttttca attatcaagc caaaaacaaa tgacagaggc
     1321 acaagacatt acactcaaaa ggatattcaa aatattcgaa tgattaagta tttgctaaaa
     1381 gaaaaacgaa tgactattaa aggcgcacaa aagcaattac aaaagaacaa tgccaataat
     1441 gtcaacacaa ctagtgtaat agataaatta aatatcataa aagcaagatt gcatgatatt
     1501 aaaaatgaaa tgaaatattt agaagagtaa aatcaaaaac aatgaaacga tacaattttc
     1561 ttttagttgc ttttatgtta acaatgccaa ttatggctgt aaacagacct aataacgtta
     1621 ttgtcataat tggagatgga atgggattaa atcatatatg cacattaaat agtcctaata
     1681 attttgaacg agctcaagca atcggattat caaaaactta ctctgctaat agtcctgtca
     1741 cagattctgc tgcaggagga actgcaatag cttgcggtgt aaagacaaac aatggtatgt
     1801 taggtgtatc gcaagactct attccggtga aatctattct tactatatta aaagagaatg
     1861 acttatcgac aggtatagtt gtaagttgcc gtatcaatca tgccacaccg gctgcttttt
     1921 atgcgcacca acctaacaga ggtatggaca gtgccatcgt gtcagattta tacaaatcaa
     1981 atatagatgt gattgttgga ggtggagcaa aatttgttag cacagattca ttgaaaaacc
     2041 ttggatatga agtatgcaac aatcttgacg atttaaaatc agtaaaaaaa gtcggtaaaa
     2101 ttgcttgttt acttgccaaa gaagatatgc ctgttgccga acaaagaaaa gatacaattc
     2161 cacaagcagt acaacaagca ctaagattat tatcgcaaaa taaaaaagga tttttcttga
     2221 tgattgaagc ttctcaaata gattgggcat ctcacggaaa caatgcaaaa tacttaaaga
     2281 ctgagattca agatataaat gctgtacttg gcgcttgtat ggattttgct gatgcaaacc
     2341 cggggacatt aattattgta actgccgacc acgaaacagg tggagtaagc ttattagaga
     2401 acgaaaaaga gtataaattc tcaacaggaa agcataccgg agtgatggtt cctatatata
     2461 gctatggtac aggagccgaa aacttctcga aaatacaaga aaatactgga tttaaaaata
     2521 aaatattgga tttaatgcat atttcaaaat aaattcatta actttgcagc cgtttttgac
     2581 gcaatctctg tcggagcaga ggaatccggt atattgcgca gcaagtcaaa ccgaaaaaca
     2641 aaatttaaaa tggacttaat caaaatcgcc gaagagtcgt tagcgacagg caaagaaaaa
     2701 actcttccct catttaagag cggagacacc gtaacgattt catataaaat cgttgaaggt
     2761 aacaaggaac gtattcagca atatcgtggt gttgttatta aaatagcagg tcatggtgac
     2821 aaaaaacgtt tcactgttcg taaaatgtca gaaggtattg gagtagaacg tattttccca
     2881 ttggattctc cattcatcga aaaattggaa gtaaataaaa ttggtaaagt tcgtcgtgct
     2941 aaattatatt acttacgcga tttaacgggt aaaaaag
//
LOCUS       DAOPID010000195         2941 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_47057_length_2941_cov_5.080042, whole genome shotgun sequence.
ACCESSION   DAOPID010000195 DAOPID010000000
VERSION     DAOPID010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47057_length_2941_cov_5.080042"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1256
                     /locus_tag="PK641_06265"
     CDS             <1..1256
                     /locus_tag="PK641_06265"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPG55983.1"
                     /translation="KINPEVIPADGTPTLIVSNHQNCLNDPLGVLFTFRDRKPHFITR
                     ADVFALHPIANKFLRSIGLLPAFRLEHEGESALGKNEETFKMTEQELVNGCTIMMYPE
                     SGHQDKRWLGNFSLGYVKLAFEAAELSDFKTDIVLLPSCNHYSDYFGIQNEFMVKFGK
                     PIHLKDFYELYKTKPRTAQRQANALAREQIENLMLNITDLDNYEAIDFLRDSYRQEYA
                     KNHNLSTADLPSKLLVDKDFYSHLEDAKKSNSATISEIYNEAIQLRDGLKEARIDEQC
                     LQNKPSLLLNILGLLLSVILFPVWIISLWPAGIQYLLPKLFGKSLDNMFQGTFLYALN
                     ILFFIPIFYLLSLILIGIYINWWIALIYVLLLPLISLFAWYYRKYSLRFITNIRYGLY
                     KNSDKIKNLSILRKTIHEQLNNILK"
     gene            1253..2470
                     /locus_tag="PK641_06270"
     CDS             1253..2470
                     /locus_tag="PK641_06270"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HPG55984.1"
                     /translation="MKRVVITGMGIYSCLGNGLDAVKESLYNGKSGIVFDPERKEMGF
                     RSALTAKLDVPDLKKELSRNQRVYMPEQAKYAYVATAEALRNSNLDIDFLETQEVGLL
                     YGNDSSAEAVMNSIDTMREKKDTTLIGSGGIFQSMNSTVTMNLACIFKLKGMNLTVSG
                     ACASGSHAIGLGTLLIRNGLQDIVVCGGAQEVNPYAVGSFDGISAFSTRESDPTKASR
                     PFDRDRDGLVPGGGAATVIIESYESAIRRGAPILAEVCGYGFSSNGDHISVPNVDGPS
                     RSLRMAIKDANIDIREIGYVNAHATSTPVGDRNEAKAIDNVFGDYKPEITSTKAMTGH
                     EMWMAGASEVIYSMLMMKNDFIAPNINFENPDEDSAKLNIPAKRIIKHFDTFLSNSFG
                     FGGTNSTLVIKNL"
     gene            2509..2754
                     /locus_tag="PK641_06275"
     CDS             2509..2754
                     /locus_tag="PK641_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPG55985.1"
                     /translation="MEKEQLIKEINEVLVEEFEVEPEVITPDADIKQTLQLDSLSLVD
                     MVALIESSFKVKIKGTEIAQLKTFGLLYDFIYERIEK"
     gene            2755..>2941
                     /locus_tag="PK641_06280"
     CDS             2755..>2941
                     /locus_tag="PK641_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HPG55986.1"
                     /translation="MATTALFEGERIKELIPQRNPIMMVDTFYNATENDANTGLTILK
                     DNFFCENGKFTEPGLIEH"
BASE COUNT         1001 a          489 c          549 g          902 t
ORIGIN      
        1 ataaaataaa tccggaagtg attccggctg atggaacccc gactttaatt gtttcaaatc
       61 atcaaaattg cttaaacgac ccacttgggg tattgtttac atttcgcgac agaaaaccgc
      121 actttataac acgcgcagat gtctttgctt tacatcctat agctaacaaa tttttacgtt
      181 caataggatt attacctgca tttcgtttgg aacatgaggg tgaatctgcc ttaggtaaaa
      241 acgaagaaac atttaagatg actgagcaag aattggtaaa cggctgcaca ataatgatgt
      301 atccggaatc aggtcatcaa gacaaacgct ggctgggaaa tttttcacta ggctacgtta
      361 aattagcatt tgaagctgcc gaattaagcg attttaaaac agatatcgta ttattgcctt
      421 catgcaacca ttattcagac tattttggca tacaaaacga gtttatggta aagttcggaa
      481 agcctattca tttgaaagat ttctacgaat tatacaaaac taaacctcgt acagctcaac
      541 gtcaagcaaa cgctttagca cgcgaacaaa ttgaaaattt gatgttgaat attactgact
      601 tggataatta cgaagctata gactttttgc gcgattcgta tcgtcaagaa tatgctaaaa
      661 atcataattt aagtactgcg gatttgccat caaaattatt ggttgacaaa gacttttaca
      721 gtcatttgga ggatgccaaa aaatctaata gtgctactat tagtgaaata tataatgaag
      781 ccatacaatt aagagatggt ttaaaagaag cacgcattga cgagcaatgt ttacaaaaca
      841 aaccatcatt attacttaat atactaggat tattattatc agttatatta tttcctgtat
      901 ggattatatc tttatggcct gcaggaatac aatatttatt acctaaatta tttggcaaaa
      961 gcttagataa tatgtttcaa ggtacatttt tgtatgcgct taacatattg tttttcatcc
     1021 ctatatttta tcttctaagc cttattttga ttggcattta cataaattgg tggattgcat
     1081 taatatatgt attacttttg ccgctcatta gtttattcgc atggtactat agaaaataca
     1141 gtctccgttt tataacaaat atccgctacg gactttataa gaattcggac aaaataaaga
     1201 atctcagcat attacgtaag actatacatg aacaattaaa caatatatta aaatgaaaag
     1261 agtcgttata actggtatgg gcatctactc ttgtttaggt aatggtttgg atgcggtaaa
     1321 agaatctcta tataatggca aatcaggtat tgtatttgat cctgagcgca aagaaatggg
     1381 atttcgctct gcacttactg caaaattaga tgtacctgat ttaaagaaag aattgagccg
     1441 caatcaacgt gtatacatgc cggaacaagc caaatacgca tacgtagcta cagcagaagc
     1501 attacgcaac tctaatttgg atatagattt tttagaaaca caggaagttg gtttgttata
     1561 tggtaacgat agtagtgcag aagctgtcat gaatagtatt gacacaatgc gcgagaaaaa
     1621 agatactaca ttaatcggtt cgggtggtat tttccaatct atgaactcaa ccgtaacaat
     1681 gaatcttgca tgtatattca aattaaaagg aatgaacttg accgtttccg gcgcttgcgc
     1741 cagtggttct cacgctatag gcttaggtac attattgata cgcaacggtt tacaagatat
     1801 agtagtatgt ggtggtgcac aagaagttaa tccttatgca gttggaagtt ttgatggtat
     1861 cagcgctttt tctacacgcg aaagtgatcc tactaaagca tcccgcccat ttgaccgcga
     1921 tcgtgatggc ttagtaccag gaggtggtgc tgcaactgtt atcatcgaaa gttatgaatc
     1981 tgccatacgc cgtggtgctc ctattctagc tgaggtttgc ggatatggat tttcgtctaa
     2041 cggtgaccac atttcagttc ctaacgtaga tggtccaagt cgttcattaa gaatggctat
     2101 taaagatgca aacatcgaca ttcgcgaaat aggttatgta aatgctcatg ctacctcaac
     2161 tccggttggg gataggaatg aagctaaagc tatagataat gtatttggtg attacaaacc
     2221 ggaaatcacc tcaacaaaag ctatgacagg tcacgaaatg tggatggcag gtgctagtga
     2281 agttatttat tcaatgttga tgatgaagaa tgatttcatt gctccaaata tcaactttga
     2341 aaatcctgac gaagattctg caaaattgaa tatccctgca aaacgcatta taaaacattt
     2401 tgacacattc ttgtcaaact catttgggtt tggcggaaca aactcaacat tggttataaa
     2461 aaacttataa ttatattcat cattattaat aatttatcct gaaaagtcat ggaaaaagaa
     2521 caattaatca aagaaatcaa tgaagtatta gtagaagaat ttgaagtaga accggaagta
     2581 atcactcctg atgcagacat caaacaaaca ttacaattag atagtttaag cttagttgat
     2641 atggttgctc ttatagaatc atcttttaaa gtaaagatta aaggcactga aatagctcaa
     2701 cttaaaacct ttggtttgct ttacgacttt atttacgaac gtatcgaaaa atagatggca
     2761 acaacagctc tttttgaggg agagcggatt aaagaactaa ttccgcaaag aaatccaata
     2821 atgatggttg acacctttta taatgcaaca gaaaatgatg ctaatacagg tttaaccata
     2881 ttgaaagaca atttcttttg cgaaaatggt aaattcacag aaccgggact gattgagcat
     2941 a
//
LOCUS       DAOPID010000196         2892 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_48196_length_2892_cov_4.462813, whole genome shotgun sequence.
ACCESSION   DAOPID010000196 DAOPID010000000
VERSION     DAOPID010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48196_length_2892_cov_4.462813"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..2129
                     /locus_tag="PK641_06285"
     CDS             84..2129
                     /locus_tag="PK641_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005467428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HPG55987.1"
                     /translation="MAKKDNMQNLTEVVDAVKEAAVKVKEAVKSAVNSENEVETKIPE
                     GPLAEKWSNYKAHQKLVNPSNKRRLDIIVVGTGLAGASAAASLAEMGFNVLNFCIQDS
                     PRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTIKGGDYRAREANVYRLAEVSNNIID
                     QCVAQGVPFAREYGGLLDNRSFGGAQVSRTFYAKGQTGQQLLLGAYSALSRQIGKGTV
                     KMYSRYEMLDVVVIDGRARGIIARNLVSGRIERFFAHAVVVGSGGYGNAFFLSTNAMA
                     SNGSAAMQMYRKGAYFANPCYAQIHPTCIPVHGAFQSKLTLMSESLRNDGRIWVPKKL
                     EDAKALQAGKMKGRDIPEEDRDYYLERRYPAFGNLVPRDVASRAAKERCDAGFGVNST
                     GLAVFLDFSDAIKRLGKDVVAAKYGNLFQMYDKIVDENPYENPMMIFPAIHYTMGGIW
                     VDYELQTSVKGLFCIGEANFSDHGANRLGASALMQGLADGYFVLPYTIQNYLADQITV
                     PRMSTNLPEFVEAEKAVDDKIKKLMSIQGKRSVDSIHRELGLIMWDFVGMGRTKEGLQ
                     QAIDKIQALRKEFWSNVFIPGKAEDLNSELEKAIRLADFIEIGDLMARDALMREESCG
                     GHFREEYQTPEGEALRQDDKFSFASCWKYAGEGKEPVMLKEKLEYEFVKRQTRNYKS"
     gene            2134..2889
                     /locus_tag="PK641_06290"
     CDS             2134..2889
                     /locus_tag="PK641_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HPG55988.1"
                     /translation="MDKSINITLKVWRQAGPKAKGGFETYKLKDVSVDSSFLEMLDVL
                     NEQLISERKEPVVFDHDCREGICGMCSLYINGHPHGPDSAVTTCQLHMRKFKDGDTIT
                     VEPWRSNAFPVIKDLMVDRRAYDKIIQAGGFISVNTGGVPDANSIPISKVNADEAMDS
                     ASCIGCGACAAACKNGSAMLFVAAKVSQLALLPQGRVEASKRAKAMVAKMDELGFGNC
                     TNTGACAAECPKSISLTNIARLNREFICAKFKD"
BASE COUNT          874 a          458 c          652 g          908 t
ORIGIN      
        1 tgtattaatg tttgcatttg tggttgtgtt tttttgcgtg agtagtttaa tcgcataatt
       61 actgataatt aaattaacta tatatggcta agaaagataa tatgcaaaat ttaaccgaag
      121 tggttgatgc tgtaaaagaa gctgctgtta aagttaaaga ggctgtaaaa tctgctgtca
      181 attcagaaaa tgaagttgag acaaagattc ctgaaggtcc tttggctgaa aaatggagta
      241 attataaagc acatcaaaaa ttagtaaatc cttcgaataa acgtcgtttg gatataatag
      301 ttgtaggtac gggtttagcc ggagcttctg ctgctgcatc tttagcagaa atgggcttca
      361 atgttttaaa cttctgtatt caagattctc caagacgcgc ccattcaatt gccgctcaag
      421 gtggtattaa tgccgcaaag aattatcaaa acgatggtga ctctgtttat cgtttgtttt
      481 atgatacaat aaaaggtggt gactatcgtg ctcgtgaagc taacgtttat cgtttggctg
      541 aggtttcgaa taatatcatt gaccaatgtg ttgctcaagg tgttcctttt gctcgcgaat
      601 acggcggttt gcttgataac cgttcttttg gcggtgctca agtatcgcgt actttttatg
      661 ctaaaggaca aacgggacaa caactgttgt taggtgctta ttctgcatta agtcgtcaaa
      721 taggcaaggg tacagttaaa atgtattctc gttacgagat gcttgacgtt gttgtaatag
      781 acggacgtgc tcgcggtatc atcgcaagaa acttggtttc aggtcgtatc gaaagatttt
      841 ttgctcatgc agtagttgtt ggatcaggag gttacggaaa tgcattcttc ttgtcaacaa
      901 atgcaatggc atcaaacgga tcagctgcaa tgcagatgta tcgtaaaggt gcatattttg
      961 caaatccttg ctacgcacaa attcacccca catgtattcc tgttcatggt gcattccaat
     1021 ctaaattaac tttgatgtct gaatcgttgc gtaacgacgg acgtatatgg gttccaaaga
     1081 aattggaaga tgctaaagca ttgcaagcag gtaagatgaa aggtcgtgat attcctgagg
     1141 aagatcgtga ttattacttg gaacgtagat atcctgcatt tggtaatttg gttcctcgtg
     1201 atgttgcatc acgtgcagct aaagaacgtt gtgatgccgg cttcggtgtt aattcaacag
     1261 gcttggctgt atttcttgat ttttcagatg caattaaacg tttaggtaaa gacgttgtag
     1321 ctgctaaata tggcaactta ttccaaatgt atgataaaat tgtcgacgaa aatccatacg
     1381 aaaatcctat gatgatattc ccggcgattc actatacaat gggtggtata tgggttgact
     1441 acgaattaca aacttctgtt aaaggcttgt tctgtatagg tgaagcaaac ttctctgatc
     1501 atggtgcaaa ccgtttaggt gcatctgctt tgatgcaagg tttggctgat ggttactttg
     1561 ttcttcctta cacaattcaa aattatttgg ctgatcaaat tacggtacct cgtatgagta
     1621 ccaatcttcc tgaatttgtt gaggcagaaa aagctgttga cgataagatt aaaaaattga
     1681 tgtcaataca aggtaaacgt tctgttgata gtattcaccg cgaattaggt cttatcatgt
     1741 gggactttgt tggtatggga cgtacaaaag aaggtttaca acaagctatt gataaaattc
     1801 aggcattacg taaagaattc tggagtaatg tatttatccc aggtaaggct gaagatttaa
     1861 atagtgagtt agaaaaagct attagattgg ctgactttat agaaataggt gatttgatgg
     1921 ctcgtgatgc tttaatgcgt gaagaatcat gtggtggaca tttccgcgaa gaatatcaaa
     1981 ctcctgaagg cgaagctctt cgtcaagatg acaaattctc atttgcttct tgctggaagt
     2041 atgcaggtga aggtaaagag cctgtaatgc tgaaagaaaa attggaatat gaatttgtaa
     2101 aaagacaaac aagaaattat aaatcataat aacatggaca aatctataaa tataacgctt
     2161 aaagtatggc gtcaagcagg tcctaaagca aaaggcggtt ttgagaccta taaattaaag
     2221 gatgtttcgg tagacagttc atttctagaa atgttggatg ttcttaacga acaattaata
     2281 agcgagcgta aagaaccggt tgtatttgat cacgattgcc gcgaaggtat ctgcggtatg
     2341 tgctctttgt acattaacgg acatcctcat ggtccagaca gtgctgttac aacatgccaa
     2401 ttgcatatgc gtaagtttaa agacggtgat actattactg ttgagccatg gcgttcaaat
     2461 gcatttcctg taattaaaga cttaatggta gatcgtcgtg cttatgataa aataattcaa
     2521 gcaggaggtt ttatatctgt taatacaggt ggtgtacctg atgcaaattc aattccaatt
     2581 tctaaagtta atgctgacga ggcaatggat tcagcttctt gtataggttg tggagcatgt
     2641 gctgcagcat gtaagaatgg ttctgcaatg ttgtttgtag cagctaaggt tagtcaattg
     2701 gctttattgc cacaaggacg cgtagaggct tctaaacgtg caaaagcaat ggttgcaaaa
     2761 atggatgagc ttggttttgg taattgtacc aatacaggtg catgtgctgc tgaatgcccc
     2821 aaaagcatat cattaacaaa tatagctcgt ctaaaccgcg agtttatttg cgctaaattt
     2881 aaggactaat ta
//
LOCUS       DAOPID010000197         2891 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_48215_length_2891_cov_4.240832, whole genome shotgun sequence.
ACCESSION   DAOPID010000197 DAOPID010000000
VERSION     DAOPID010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48215_length_2891_cov_4.240832"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..1374
                     /gene="serB"
                     /locus_tag="PK641_06295"
     CDS             151..1374
                     /gene="serB"
                     /locus_tag="PK641_06295"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HPG55989.1"
                     /translation="MTSEIILINISGDDRPGVTASITDILGRYNAVILDIGQADIHHT
                     LSLGILFKMDNEIQSGHILKEILFKCSELGVQVRFTPIPEDRYNNWVGRQGKSRYVTT
                     ILGRVITANQLSLVTKSILDQELNIDSIKRLTGRPPLDENNSDEVRSCIELSLRGELK
                     DKNIISSRFMEISAKEGIDISFQKDDMYRRARRLICFDMDSTLIKTEVIDELADKAGV
                     GSEVRAITESAMRGEIDFKESFTKRVALLKGLDESVMVDIAEHLPLMDGAERLMKVLK
                     KFGFKIAILSGGFTYFGEYLQKQFGIDFVYANNLEIENGKLTGRYLGDIVDGKRKAEL
                     LHLIAQVEKIDLEQVVAVGDGANDLPMLNLAGLGIAFHAKPKVIANASQSISTIGLDG
                     ILYFLGFRDIHMDIK"
     gene            complement(1367..2086)
                     /locus_tag="PK641_06300"
     CDS             complement(1367..2086)
                     /locus_tag="PK641_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPG55990.1"
                     /translation="MKAMIFAAGLGTRLKPLTDTTPKALIPVKGKTLLEHVIIKLKDA
                     GFKDIVINIHHFGEQIIDFLQANNNFGINIVISDERGMLLETGGGIKKAANLLGNEPF
                     LIHNVDILSNADLGALYRANTQSNAVATLLVNKRESSRYLLFAESRLNGWTNVKTGEI
                     KSPYTNIDIERTDRFAFSGIQVFSPQLFKYMDNFPDKFSIIDFYLAICNKEKISYYIQ
                     EDLKLLDVGKIDSLEQAESFI"
     gene            complement(2083..>2891)
                     /locus_tag="PK641_06305"
     CDS             complement(2083..>2891)
                     /locus_tag="PK641_06305"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HPG55991.1"
                     /translation="GEPWFIDFQGGRKGPIYYDLASFIYQAKAKFSEDTKESLIKTYL
                     EALKPYRDISFDKFKKEFSYFVLFRVLQTLGAYGFRGLIEHKSHFIQSIPYAIANLKQ
                     TLNTYSFSEFPYLKKLLEQLINKYQISETTDSDQLIVRVFSFSYKKGIPDDYSGNGGG
                     FVFDCRALHNPGRYDEYKQLTGLDKPVIDFIENNGEMQLFLNNVFAIADSSVERYIKR
                     GFTNLMFSFGCTGGRHRSVYAAQKLAEHISKKYGVKVALTHREQSIMTSL"
BASE COUNT          920 a          440 c          533 g          998 t
ORIGIN      
        1 ttacagaagc ctcataagtt tatgcttatg gggctttctt atttatatta cttcaaatgt
       61 atattcacta ttatttaatt tagataatgc agcaaatttg tgtgttacta aaaaaacaaa
      121 tatctttgca aacgttataa atgacttatt atgacaagtg aaattatact tataaatata
      181 tcgggcgatg atcgtcctgg agtaactgca tcaataacag atatattagg acgttataac
      241 gctgtaatat tggatattgg tcaggctgac attcatcata cactatcttt gggcatccta
      301 tttaaaatgg ataacgagat tcaatccgga catatattga aagaaatatt atttaaatgt
      361 tctgaattag gtgtgcaagt gcgttttact ccaataccgg aagatagata caataattgg
      421 gttggtcgtc agggcaaaag taggtatgta actactattc ttggtcgtgt tattacagct
      481 aatcaacttt cattagttac aaagtctatt ttagatcagg aactaaatat tgattcaatt
      541 aaaaggctta caggtcgtcc acctttagat gaaaataatt ctgatgaagt acggtcgtgt
      601 attgagcttt ctttacgcgg tgaattgaaa gataaaaata taatatcatc acgttttatg
      661 gaaatttctg caaaggaggg aatagatatt tcatttcaga aagatgatat gtatcgtcgt
      721 gcaaggagat taatatgttt tgatatggat tctacattaa ttaaaacaga agtgattgat
      781 gaacttgccg ataaggccgg agttggctcg gaagtaagag ctattacaga atctgcaatg
      841 cgtggtgaga ttgattttaa agaaagtttt actaagcgtg tggctttgct aaaaggtctt
      901 gatgaatcag ttatggttga tattgctgaa catttaccat taatggatgg cgcagaaaga
      961 cttatgaaag tgttgaaaaa gttcggtttt aagattgcaa tactttcagg aggtttcaca
     1021 tatttcggcg aataccttca aaaacaattt ggaattgatt ttgtatatgc aaataacttg
     1081 gaaatagaga atggaaaact aacaggccgc tatttaggtg atattgtgga tggaaaacgt
     1141 aaggcggagt tattacattt aattgctcaa gtagaaaaga ttgatttaga gcaggtagtc
     1201 gctgtaggtg atggagcaaa tgatttgcca atgcttaatt tagcaggact tggaatagca
     1261 tttcatgcaa aaccaaaggt tatagctaat gcaagtcaat caatatcaac aataggattg
     1321 gatggtattt tgtatttcct aggttttagg gatatacaca tggatattaa ataaatgatt
     1381 cagcttgttc gagtgaatca attttaccta catcgagtag ttttaagtct tcttgtatat
     1441 aatagctgat tttttcctta ttgcatattg caagataaaa atctataata gagaacttgt
     1501 caggaaaatt gtccatgtat ttgaatagtt gtggagaaaa tacctgaatg ccgctaaagg
     1561 caaaacgatc ggttctttct atgtcaatat tagtgtatgg acttttaatc tctccggttt
     1621 ttacattagt ccatccattt aatcttgatt ctgcaaatag taggtaacga gatgattctc
     1681 gtttgtttac cagtaatgtt gcaactgcgt tagactgcgt attagcccta tataaggcgc
     1741 ctaaatcagc atttgataga atatcaacat tgtgaatcaa gaatggctca tttccgagta
     1801 aattggcggc tttttttata cctccaccgg tttcaagtaa cattccacgt tcatcagaaa
     1861 taacgatatt gataccgaaa ttattgttag cttgtaaaaa gtctattatt tgttctccga
     1921 aatgatgtat atttataact atatccttaa aacctgcatc ttttagtttt ataataacat
     1981 gctcaagtaa agttttacct ttaaccggaa ttaatgcttt tggcgtagta tcggtcaaag
     2041 gttttagacg agtgcctaat cctgccgcga aaatcattgc tttcataaac tagtcattat
     2101 tgattgctca cgatgcgtaa gcgctacttt aactccgtat ttttttgaga tatgttctgc
     2161 gagtttttgc gctgcataaa cagatctgtg acgtccgccg gtacaaccaa aactaaacat
     2221 tagattagta aatcctcgtt taatatatct ttcaacagac gaatcggcta ttgcaaatac
     2281 attatttaaa aacaattgca tttcaccatt gttttctata aaatcaatta ccggtttatc
     2341 taagcccgta agttgtttgt attcgtcgta tcttccaggg ttatgtaatg ctctacaatc
     2401 aaacacaaaa ccacctccat tacctgaata atcatcaggt attccttttt tataagagaa
     2461 actgaatacg cgaactatca attgatcaga gtcggttgtt tcggatatct ggtacttatt
     2521 tattagttgc tctaaaagct tttttaggta cggaaattcc gagaaagagt atgtgtttaa
     2581 tgtctgtttc aggtttgcga tggcgtatgg tatgctttgt ataaaatgtg atttatgctc
     2641 tataagtcct ctaaaaccat atgctcctaa tgtttgtagc actctaaaaa gcacaaagta
     2701 tgaaaattct ttcttaaatt tatcgaaaga tatgtctcta tacggcttaa gtgcttcaag
     2761 ataagttttt attagagatt ccttcgtgtc ttctgaaaat ttagcttttg cttgatatat
     2821 aaacgatgca agatcgtaat atattggacc ttttcttcct ccttgaaaat caataaacca
     2881 tggttctcca t
//
LOCUS       DAOPID010000198         2891 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_48217_length_2891_cov_4.000705, whole genome shotgun sequence.
ACCESSION   DAOPID010000198 DAOPID010000000
VERSION     DAOPID010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48217_length_2891_cov_4.000705"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..2425)
                     /locus_tag="PK641_06310"
     CDS             complement(164..2425)
                     /locus_tag="PK641_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPG55992.1"
                     /translation="MFTEDLNQPQCEAVMYNEGPSLIIAGAGSGKTRVLTYKVAYLLH
                     LGLAPHQILALTFTNKAAREMRERIALMVDAEQAKRLAMGTFHSVFARILRTEANLVG
                     LKSDYTIYDTADSKNLIKSILKEMSLDEKIYKVNLILSRISSAKNNFIAANLYAGSEH
                     VKQDFLMQIPRVKDIYSTYVQRCMSANAVDFDDILLLTTQLFKEHPEVLEKYQNRFQF
                     ILVDEYQDTNLVQFILIKSLAEKNHKICVVGDDAQSIYAFRGANIENILKFTKNYPEA
                     KLFKLERNYRSSRNIVAAAGSLIAKNRRQIPKNIFSEKEEGDKIKILPAYSDVEEALI
                     VSNKISDLKNKFGCDWNDFAVLYRTNSQSRLIEDTLRKRNIPYVIYGGLSFYQRKEVK
                     DVLAYIRLILNTDDEEAFKRVVNYPIRGIGDTTILKLSSTANLHHVSLFEVASNVLNY
                     ALPVNAGTAAKLKSFVNLINELKSLESTMSLYEFAREILLRSNILQTLNEENTAESES
                     ARENIEELLNSIHLFEDMSREEGRDGVKLSDFMAEVALLTDMDLDKNVETSRVTLMTI
                     HSAKGLEFKNVFVTGLEESLFPSIHAMENDTELEEERRLLYVAITRSEEHCFLSYAKS
                     RYRNGSLQYPSRSRFIDDIDPRYLEFPTDKNMSQKPIDFSKRDESFFNSFSKPQLPKR
                     LVPISNNPKQITNNNTNLSVKEGMRIEHERFGIGTINRIDDTPDGTRIYVDFETSGSR
                     VLLLKFTKFKILS"
     gene            complement(2439..>2891)
                     /locus_tag="PK641_06315"
     CDS             complement(2439..>2891)
                     /locus_tag="PK641_06315"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPG55993.1"
                     /translation="VNNDESNLGVSLISAGNNTSYLGSAKDIFPGQDHITEYLPLDIS
                     NIVDWQDENLSPQNLNVSFKYKGGLVDISKQTINTCKLSYSNSILTVSGLSGVSNCEI
                     YDMSGLLHSSTLSDNDICKILVPKLSNGLYLLKISNDLGDSIHKWVKY"
BASE COUNT          959 a          533 c          395 g         1004 t
ORIGIN      
        1 cttaagtcgc tggaatgtac atcaacattt tccaacaaat ttccattagt atctcgtata
       61 taaccggata caacaaaatc tttcttatcc gttagtgagg gtaaggctaa tgatattaga
      121 aatatgagga aggtaaattt cactttattc tataacgtaa aaactaagat aatattttaa
      181 acttagtaaa tttaagcaac aacacacgac tacctgaagt ttcaaaatca acatagattc
      241 ttgtaccatc aggtgtatca tctattctat tgatagtacc tattccaaag cgttcatgtt
      301 caatacgcat tccttcctta accgataaat ttgtattatt attggtaatt tgctttggat
      361 tattacttat aggtactaaa cgttttggta attgaggttt tgaaaaagaa ttaaaaaagc
      421 tctcatcccg tttagaaaaa tctatcggtt tttgtgacat atttttatcc gtgggaaatt
      481 caagataacg cgggtctata tcgtcaataa aacgactacg agatggatat tgaagtgaac
      541 catttctata tctactttta gcatagctca aaaaacaatg ttcttccgaa cgtgttattg
      601 ccacataaag caaacgcctt tcttcttcta gttctgtgtc attttccata gcatgaatac
      661 ttggaaacaa ggattcttcc aatccggtaa caaacacatt tttaaactcc aaacctttgg
      721 ctgaatgtat agtcataagt gttacacgtg atgtctctac atttttatct aaatccatgt
      781 ctgtaagtaa tgcgacttca gccataaaat ccgatagttt aacaccatca cgtccttctt
      841 ctctactcat atcctcaaac aagtgaatac tattaagtaa ctcctcaata ttttcacgag
      901 cggattctga ttctgcagta ttttcctcat ttaaagtttg aagtatatta gaacgcaaca
      961 atatttctcg tgcaaattca tacagagaca ttgttgattc caatgatttt aattcattga
     1021 ttaaattaac aaaagattta agttttgctg ctgttcctgc attaacaggt aaagcataat
     1081 tcaatacatt tgaagcgact tcaaataatg atacatgatg caaattagca gtgctactta
     1141 attttaatat tgtagtatcg ccaatacctc ttataggata atttacaacg cgcttgaacg
     1201 cctcttcatc atctgtattt aaaattaatc ttatatacgc cagaacatct tttacctctt
     1261 ttctttgata aaaagataga cctccatata taacatatgg aatatttcgc ttacgtagag
     1321 tatcttcgat caaacgagat tgagaatttg ttctatataa tacggcaaaa tcattccaat
     1381 cgcatccaaa tttattcttc aaatcactaa ttttatttga tacaattaat gcttcttcta
     1441 catcagagta ggcaggtaaa attttaattt tatcaccttc ttctttttct gaaaaaatat
     1501 ttttaggaat ttgtcgtctg ttttttgcga ttaaacttcc cgctgcggca acaatattac
     1561 gagatgaacg ataattcctt tccaatttga ataactttgc ctcaggatag ttctttgtaa
     1621 attttaatat attctcaata tttgcacctc taaaagcata tatactttgc gcatcatcgc
     1681 ctactacaca tattttgtga ttcttttcag ctaaactttt tattaaaata aactgtacca
     1741 aatttgtatc ttgatactca tcaactaaaa taaattgaaa tcggttctga tatttttcga
     1801 gaacttcagg atgttcctta aacaattgag tagtcaacaa taatatatca tcaaaatcca
     1861 cagcatttgc agacatacat ctttgtacat aagtagaata aatatccttt acgcgaggaa
     1921 tctgcattaa aaaatcttgt tttacatgtt cacttccggc atataagttt gcagcaataa
     1981 aattattctt cgcactagat attcgactca atattaaatt aactttgtat atcttttcat
     2041 ccaaagacat ctctttaaga atcgacttta ttaaattttt tgagtctgct gtatcatata
     2101 ttgtataatc agatttcaat ccgacaagat ttgcttcagt gcgcagtata cgtgcaaaaa
     2161 cagagtgaaa tgtacccata gctaagcgct tagcttgttc ggcatcaacc attaaagcaa
     2221 tacgttcacg catttcacga gcagctttat tagtaaaagt caaagccaat atctggtgcg
     2281 gagctaaacc caaatgcaac aaatatgcaa ctttataggt taatacacgc gtttttcctg
     2341 aaccggctcc ggctattatt aacgaaggac cctcattata cataacagct tcgcactgcg
     2401 gttgatttaa atcttctgta aacataaaac taaaaaaatc aatatttcac ccatttatgg
     2461 atgctatcac ctaaatcatt actaatcttg agcaaataca agccatttga taatttaggt
     2521 accaagattt tgcatatatc attatcagat agagttgacg aatgcaacaa tcctgacata
     2581 tcatatattt cgcagtttga tacacctgat aacccgctta cagtcaatat gctatttgag
     2641 taagatagtt tacaagtatt aatggtttgt ttcgatatat cgactaaacc acctttatac
     2701 ttaaaactta catttaagtt ttgtggagat aaattttcat cttgccagtc aacaatattg
     2761 cttatatcta atggtaaata ctcagtaata tgatcttgcc ccgggaaaat atcttttgca
     2821 gagccaagat atgaagtatt atttcctgct gaaatcaaag atactcctaa attgctttca
     2881 tcattattta c
//
LOCUS       DAOPID010000199         2865 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_48801_length_2865_cov_3.101779, whole genome shotgun sequence.
ACCESSION   DAOPID010000199 DAOPID010000000
VERSION     DAOPID010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48801_length_2865_cov_3.101779"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2865)
                     /locus_tag="PK641_06320"
     CDS             complement(<1..>2865)
                     /locus_tag="PK641_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55994.1"
                     /translation="TTLPTITWTAYDEYQKITKVEGGVNGDTRITVKAQSGAMTVYVL
                     SFSVAKSSNSLLSDIKVGGVSLADFASETFEYGYVLASGTTILPEISYTKADSYQKVS
                     VLKNGVNGITNINVTAEDGTESIYKISFSVEKSENAGLKDIKVGGVSLADFDPNTLNY
                     TYVLAAGVTQCPDVTVEKNEGQSVTIVVPKLIGIARIEVVPEIGSKNVYTINFTYQQS
                     DNSKLADLLVDGVSISGFSPETTQYNYVLPQGTTELPAITYLKAEDSQIVELNENGVN
                     GTSTISVKAENASVTTYQITFSVVKSSNAYLSDIQIAGASLIGFDETTYSYNYVLPEG
                     TTSLPLFKAIKADQGQVISVSLPSLQGTASVVVTSEDASATNNYFVNFAFESKSDAAL
                     QNISIDGIALTDFSPSVYSYNITLPVGSGKPVLSYEKADGSQVVVLSTDINKTVLAVY
                     AEDGSYNEYVINYSYSANTSALLADLQVYDNASQTFVSLADFSSDKFDYSKILSLGST
                     DLPTVNPIPATAGQIITITYGKVNEITNIHVVAEDGVTTADYTIEFSVEKSTENSLIN
                     ISVGGNDVPGFDPGILDYVVTLPYGTTTVPAITYDKNYFGEMVQLTASDINSTSYLKV
                     YAEDGSSRTYSLQFNVSESGLDNVLYTIMLDDEAISGFSENQYTYDVVLPFGTTQMPD
                     ISYVKRYAEQTVVTTISGMSKIELRVKSNQDGVADAIYSINFTVAQYQSTVLSDIKLD
                     NVSLTGFDSHKTKYVVSIANKPASIDAVSPASNIIAEKIIDTNNHVRFTVSNTFNSET
                     ITYDLYFHYTNDVIPNGEFTQWTTAKYNSGPKPVGWKVPADAVEKSSTGWEWMFGTAK
                     TGSEVANSSNQAARLKTTYWAVLAGTIPSVMTIGDISCELKVAIGSTTNFSGGITFRN
                     TPDFAAIRYNFVKKQGDGALFAFRFDSDARDYVKTEATSG"
BASE COUNT          907 a          529 c          463 g          966 t
ORIGIN      
        1 agcctgaggt tgcttctgtt ttaacataat cacgtgcatc actatcgaaa cggaatgcaa
       61 ataaggctcc atctccttgt tttttaacaa aattatagcg aattgccgca aaatcgggag
      121 tatttctaaa agttatacca ccgctaaaat tagtagtaga acctatcgct actttcaatt
      181 cgcaactgat gtcgcctatt gtcataacag atggtattgt gcctgccaat acagcccaat
      241 aagttgtttt aagacgggca gcttgattac tactatttgc aacttctgaa ccggttttag
      301 ctgttccaaa catccattcc caacctgtgc tactcttttc tacagcatct gctggcacct
      361 tccagcctac aggcttaggt ccactattgt atttagctgt tgtccattgt gtaaattcac
      421 cattaggtat aacatcattt gtataatgga aatacaaatc ataagtaata gtttctgagt
      481 taaaggtatt agataccgta aatcttacat gattatttgt atctataatc ttttctgcaa
      541 ttatgttact tgcaggacta acagcatcaa ttgatgccgg tttgttagcg atagacacaa
      601 catattttgt tttgtgagaa tcaaaaccag tcaatgaaac attatctagt ttaatatctg
      661 ataaaacagt gctctgatat tgagctacag tgaaattaat tgaataaata gcatccgcaa
      721 caccatcttg attagattta actcttaatt ctatttttga catcccggaa atagttgtaa
      781 caactgtttg ttctgcatat ctcttaacat aagagatatc aggcatttga gtcgttccaa
      841 acggtaatac tacatcgtaa gtatattgat tttctgaaaa accgcttatt gcttcatcat
      901 caagcattat tgtatacaat acgttgtcta gacctgattc cgaaacattg aactgcaaag
      961 aatatgtgcg agaagaacca tcttcagcat atacctttag atatgatgtc gaattaatat
     1021 ctgatgcggt taattgcacc atttcgccaa agtaattttt atcataagta attgcaggaa
     1081 cagttgtcgt accataaggc aaagtcacaa cataatctaa tatgccggga tcaaaacccg
     1141 gaacatcatt acctccaact gaaatattta tcaaagaatt ttcagtagat ttttcaacag
     1201 aaaactcaat tgtataatct gcagttgtta ctccgtcttc tgcaactaca tgaatatttg
     1261 taatttcatt aacttttcca taagtaattg taataatctg accggcagtt gcaggtatcg
     1321 gattaacagt tggtaggtct gtacttccta aagataaaat ttttgaataa tcaaacttat
     1381 cagaagagaa gtcggctaat gatacaaatg tttgagaagc attatcgtaa acttgtaagt
     1441 ctgctaataa tgcacttgta tttgcgctat atgagtaatt aattacatat tcattatagc
     1501 ttccatcttc tgcgtataca gctaatactg tcttattaat atcagttgat aatacgacta
     1561 cttgtgagcc atcggctttt tcgtatgata aaacaggttt tccgctgcct acaggcaatg
     1621 taatgttata cgaatacaca cttggagaaa agtctgtcaa tgcaatacca tctattgaaa
     1681 tattttgaag agcagcatct gacttacttt cgaatgcaaa atttacaaaa taattattgg
     1741 ttgcagatgc atcttctgat gttacaacaa cagatgcagt tccttgtaat gaaggtaatg
     1801 atactgaaat tacctgaccc tgatcagcct ttattgcttt gaacaacggc aaacttgtag
     1861 taccttcagg taatacataa ttatagctat aggttgtttc gtcgaatcct attaatgaag
     1921 cacctgcaat ttggatatct gataaatatg catttgaaga tttaacaaca gagaatgtaa
     1981 tctgatatgt tgttactgaa gcattttctg cttttacaga tattgtactc gtgccgttta
     2041 caccattctc gtttaattca actatttgag aatcttcagc tttaagatat gtgatagcag
     2101 gtaattctgt agtaccttga ggcaatacat aattatactg agtagtttca ggtgaaaaac
     2161 cgctaattga aactccatct accaacaaat cggcaagttt agaattatct gattgctgat
     2221 atgtaaagtt tatagtataa acatttttag atccaatctc aggcacaacc tcgatacgag
     2281 ctatacctat taattttgga actactatag taactgattg tccttcgttc ttttctacag
     2341 taacatcagg acattgtgtt acgcctgcag ctaatacata agtatagttt aatgtattag
     2401 ggtcgaaatc agctagcgaa acaccgccta ctttaatatc ttttaaacct gcattctctg
     2461 atttttcaac agagaaagat attttgtaaa tcgattcggt tccatcttct gcagtcacat
     2521 taatattagt gataccatta acaccattct tcaatactga taccttttgg tatgaatcag
     2581 ctttagtata gctgatctca ggaagaatag ttgttccact tgctaatacg tatccatatt
     2641 caaaagtttc agaagcaaaa tctgctaatg atactccacc tactttgata tcactaagta
     2701 atgagtttga tgatttagct actgagaatg ataatacata tacagtcatc gctcctgatt
     2761 gagcttttac cgtaatacgc gtatctccgt ttacaccacc ttctacttta gtaatctttt
     2821 ggtattcatc ataagccgtc caagtaatgg taggtaatgt agttg
//
LOCUS       DAOPID010000200         2830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_49601_length_2830_cov_3.940541, whole genome shotgun sequence.
ACCESSION   DAOPID010000200 DAOPID010000000
VERSION     DAOPID010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49601_length_2830_cov_3.940541"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..1139)
                     /locus_tag="PK641_06325"
                     /pseudo
     CDS             complement(57..1139)
                     /locus_tag="PK641_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            complement(1230..1817)
                     /locus_tag="PK641_06330"
     CDS             complement(1230..1817)
                     /locus_tag="PK641_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPG55995.1"
                     /translation="MATKIRLQRHGRKGYAYYHIVIADSRAPRDGRFVERIGSYNPNT
                     NPATVDINFELALKWVNSGAQPTDTVRNILSDEGVMLKKHLMGGVAKGAFTEEVAEKK
                     FQAWKAEKAKSIAKISEKANASAAEKAKARLTAEKAVNAAKAAEAAQKRAEKLAAEQE
                     AVKAAQPIVEAAAEEAPVVEAAAEEASVVETPAAE"
     gene            complement(1938..>2830)
                     /locus_tag="PK641_06335"
     CDS             complement(1938..>2830)
                     /locus_tag="PK641_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55996.1"
                     /translation="NNTPITYKVEYDAQNRIKSFSASQVVIDDEGKKEKIKINTLQPK
                     YNRKTGEHMGITTFDGTLVRIIMPVYDKINSISDKMAAETFCKTENLVGGFIKMKTET
                     TEEVQLSMNTDGTINFEGKSVSSRPFSFLKAIADSNNIKFPSLVSENHTQFKSLVKIN
                     SDGVITLLDWEGSILLEKNKGYDNYAYILADNIKHQIEVLEEDSYGQPQKIRSSVSMD
                     IKNNWGNITKWNYIEIFGDSKNNIKLNEAKKARKEAAMAADQSIDFSFDWDVSTKYDD
                     RGNWTRMELGPYIFERTISY"
BASE COUNT          840 a          578 c          430 g          982 t
ORIGIN      
        1 aaatcttctt ggaaacgcat catcgaaaaa acaccatatt tagcatattc gggctaatct
       61 tccggacgta tttccactac catacccgaa ttagcccatt ttgaacctct gtttgatggt
      121 gacattccgt tcacaacaac ttgttgtggt ccacttgcag caggtactac aaaaccaccc
      181 gggcacatac aaaatgaata aactccccta ttttgaattt gcgttacaaa attgtattca
      241 gcaggaggca aatacaatcc tcttccttgt acattatgat attgaatttg gtcaattata
      301 atttgaggat gctctaaacg cactcctacg gcaattcctt ttgactccat tgtaactccg
      361 cgtttatcta aaaaggcata cacatcgcgt gccgaatgac ctgttgccat aattaccggt
      421 ccatggtatt catcaccatt ggcagtaatt actcccttta cagattcttt attgagtatt
      481 aaatcaacaa ctttagtttc aaaatggact tctccgcccg attgtataat tctttcacga
      541 attttacgaa taaggtcagg taatttatct gttcctatat gtggatgcac gtcaaccaaa
      601 atattaggat tagctccatg catacaaaaa atcttcaata tgcgttcgtt atttcctttc
      661 tttttacttc gtgtatataa tttaccatca gaataagccc cggcaccacc ttctccaaaa
      721 caataatttg attcgggatc aactatttga tctcgcgaaa ttaatgcaat gtcgcgtttt
      781 cgagtagata catcttttcc tctttcaagt aaaattggtt ttagtcctaa ttcaattaaa
      841 cgaagagcgg caaacaaacc tgcaggacct gcaccaacaa taataacagg cttcgatttt
      901 gacacatcac catactcagc tatacaataa tctatttgtg gagccgattc attttcaaac
      961 acgcgtatac tcagattaac ataaactgtt ttctgacgtg catcaataga acgttttaaa
     1021 atttgaaccg ccttgattga tacagaattt aagccgtatt cttttgatac aaaatcaatc
     1081 aaaacacgtt catcagctgc tatagccggt aatacacgca gattatattc atgaatcata
     1141 cttcaatatt tgtgatgtaa acaataagtt ttatcgaatg aaaaagcccc ggcttttgcc
     1201 gaggcttaaa tattgatacc gaaacaaaat tattcagcgg caggagtttc aacaactgaa
     1261 gcttcttcag cagcagcttc aacaactgga gcttcttcag cagcagcttc aacaatcggt
     1321 tgagcagcct ttacagcttc ttgttcagca gctaattttt cagctctttt ctgtgcagct
     1381 tcagcagctt tagcagcgtt aacagctttt tctgctgtta gtctggcttt tgctttttca
     1441 gcagcagaag cattagcttt ttctgaaatc ttagcgattg atttagcttt ttcagctttc
     1501 catgcttgga attttttttc agctacttct tcagtaaatg cgcccttagc tacaccaccc
     1561 attaagtgtt tcttcaacat tacgccttca tccgaaagga tattacgtac tgtatctgtt
     1621 ggttgtgcgc cggaattaac ccatttaaga gctagttcga agttaatgtc taccgttgca
     1681 ggattggtgt tagggttgta agaaccaatt ctttcgacaa acctgccatc acgtggcgct
     1741 ctactgtcag caataacgat atgataatat gcatatcctt tgcgaccatg tctttgcaat
     1801 ctaattttag ttgccatttt ttattgtttt aaattattac ttctgtgcat tactcgaatg
     1861 cgggtgcaaa gatacaaaat aaattctatc tatcaacaat ttatatgtgt ttttgcaatg
     1921 aagaaataat aatctattta atagcttata gttctttcaa atatatatgg acctaactcc
     1981 atgcgagtcc agtttcctct atcgtcatat tttgttgaaa catcccaatc aaaagaaaaa
     2041 tcgatagatt gatcggcagc cattgcagct tctttacgag cttttttagc ttcattaagc
     2101 tttatattat tttttgaatc gccaaaaatc tcaatataat tccacttagt tatattaccc
     2161 caattgtttt taatatccat agaaacactg gaacggattt tttgaggttg tccgtaactg
     2221 tcttcttcta agacttcaat ctgatgttta atattatcag ctaaaatata tgcgtaatta
     2281 tcgtagccct tatttttttc taataaaata ctcccttccc aatcaagtaa agtaataact
     2341 ccatcagagt tgatttttac caaagattta aattgagtat gattttcact aaccaaagaa
     2401 ggaaatttaa tgttatttga atcagctatt gccttaagga aagagaatgg acgtgatgat
     2461 accgatttac cttcaaaatt aatagtacca tcagtattca ttgacaactg cacttcttca
     2521 gttgtttccg ttttcatctt aatgaagcca ccaactaaat tctctgtttt acaaaatgtt
     2581 tctgctgcca ttttgtcgga aattgaattt attttatcat aaacaggcat aataatacgt
     2641 accaaagtgc catcaaaggt cgttataccc atatgttctc ctgtttttct attgtactta
     2701 ggctgcagtg tattaatctt tattttctct tttttacctt cgtcatctat tacaacttga
     2761 gatgcagaaa aacttttaat tctattttgt gcatcatatt caactttgta tgtaataggc
     2821 gtattattta
//
LOCUS       DAOPID010000201         2776 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_50967_length_2776_cov_3.749357, whole genome shotgun sequence.
ACCESSION   DAOPID010000201 DAOPID010000000
VERSION     DAOPID010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50967_length_2776_cov_3.749357"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..605)
                     /locus_tag="PK641_06340"
     CDS             complement(93..605)
                     /locus_tag="PK641_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG55997.1"
                     /translation="MKKTLLVLSVLMSLLLGSCTNGFEKQSPYIQIFDSKINGADFAH
                     NDTLFIGDTLQIYAALNPYYNKLIEFDISCDRNYIKDSVFSDADFETFCDVSQSNRSE
                     GKYVFKNLNVGDILIISPIQFIAVNAKNPDTDDITITFSLESDVDMEPLYNPFVFSTH
                     VKIVNPDNNP"
     gene            683..1357
                     /locus_tag="PK641_06345"
     CDS             683..1357
                     /locus_tag="PK641_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPG55998.1"
                     /translation="MIDGFLPKAFLFDMDGVLFDSMPCHANAWTKAFESVGIEFSEYQ
                     VYLQEGCTGSKTINDAFVAQKNRKATQAEINEIYARKSKFFEQSGDPVPMQYAKEVLD
                     CVRDKGIKIGMVTGSGQKSLLATLNHYYPGIFSKNNMVTAFDVVLGKPNPEPYLKGLE
                     KLSVSANEAVVVENAPMGVKSAVSAGIYTIAVNTGILKEDDLKQYGANLVLGGMRQLL
                     DLLSSK"
     gene            complement(1358..2632)
                     /locus_tag="PK641_06350"
     CDS             complement(1358..2632)
                     /locus_tag="PK641_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPG55999.1"
                     /translation="MSKKQVSPWAWIPSLYFGQGIPYVVAATTFSMIMYKKLGISNAD
                     AALYTSWLYLPWVIKPFWSPFVDLFKTKRWWVVSMQFIMGVALAGVGFCIPAPFFFQS
                     TLAVLWLIAFSSATHDIAADGFYMLALDEHQQSLFVGIRSTFYRIAQIVGQGLLVILA
                     GYLEQKFNNIALAWSVTFYGIAAFFICIAIYHYFILPKPNNDNAAAGNKYPIEGFIST
                     FASFFNKKGIILSLAFVLLYRLGEAQLVKIASPFMLDPIDKGGLGLSTSDVGFVYGTV
                     GIIALTVGGILGGIAAATHGLKNCLWTMVLAMNIPNLVYVYFAYAQPTNYAIICSGVA
                     IEQFGYGFGFTAFMLYLIYISKGEFKTAHYAICTGFMALGMMLPGLGAGIIQESIGYL
                     NFFIWVCICTIPGFILTALLKIDKQFGIKQKE"
BASE COUNT          922 a          487 c          478 g          889 t
ORIGIN      
        1 agaagcctcg cctttaaatt cgtctctagc acacgaatat ggctttaatg cccaaatatt
       61 aggtcttact aatttttcta aatttatcat tcttatgggt tattatccgg gtttacaatt
      121 ttaacatgag tgctgaatac aaaagggtta tataagggtt ccatatctac atcactttct
      181 aaagagaacg ttattgtaat gtcatctgta tcaggatttt ttgcgtttac tgcaataaac
      241 tgaataggac ttataattaa tatatcaccg acattaagat ttttaaaaac atacttacct
      301 tcagaacgat ttgattgtga tacatcgcaa aatgtttcaa aatctgcatc agaaaaaaca
      361 gaatccttta tatagttgcg gtcgcaagat atgtcaaatt caatcaactt attgtaatat
      421 gggtttagcg cagcatatat ctgtaatgtg tctccaataa atagtgtatc gttgtgagca
      481 aaatctgcgc catttatttt ggaatcaaat atttgtatat atgggctttg tttctcaaat
      541 ccattagtac atgagcctaa taatagactc attaatacgc ttaaaactaa aagtgtcttt
      601 ttcatatggc aatcgctttt tgcaaaggta caaactattt gaaaaataaa tgtacctttg
      661 catctcaaaa aatgtttata agatgattga tggttttttg ccaaaggctt ttttgtttga
      721 tatggatggc gttttgtttg attcaatgcc gtgtcacgca aatgcttgga ccaaagcatt
      781 tgaaagtgtc ggtatcgaat tttccgaata ccaagtgtat ctacaagaag gttgtacagg
      841 atccaaaaca attaacgatg cttttgttgc tcaaaaaaat cgcaaagcaa cacaagcaga
      901 aattaatgag atttacgcaa gaaaaagtaa attctttgaa caatcaggtg atcctgttcc
      961 aatgcaatat gccaaagaag ttttagattg tgttcgcgat aaaggcatta aaataggaat
     1021 ggtaacaggc tcaggtcaaa aaagtttgtt agctactcta aatcattatt atccgggaat
     1081 attttctaaa aataatatgg taacagcctt tgatgtagta cttggcaaac caaatcccga
     1141 accatattta aaaggacttg aaaaattatc tgtatcagca aacgaggctg ttgtggttga
     1201 aaatgctcct atgggcgtaa aatccgcagt ttcagcaggc atatacacaa tagctgtaaa
     1261 taccggaatc ttaaaagagg atgatttgaa gcaatatggt gcaaatttag tacttggcgg
     1321 tatgcgtcag ttactcgatt tattatcaag taagtaatta ttctttttgt ttgatgccga
     1381 attgcttgtc tatcttcaat aaagcagtta aaataaatcc cggtattgtg caaatacaaa
     1441 cccatataaa gaaattcagg tatcctatgg attcttgtat gattcctgct cccaaaccgg
     1501 gaagcatcat tcctaatgcc ataaaacctg tacatatcgc ataatgtgct gttttaaatt
     1561 cacctttcga aatatagata agatataaca taaatgcagt aaatccgaat ccgtagccga
     1621 actgttcaat tgctactcct gagcatatta ttgcataatt tgtgggttga gcataggcaa
     1681 aataaacata taccaagtta gggatattca tagctaatac catagtccac aagcaattct
     1741 ttaatccatg tgtagctgct gctatgcctc caagtatacc gcctactgta agtgctataa
     1801 tgcctactgt accgtaaacg aatcccacat cacttgtaga taagccaagt cctcctttat
     1861 caatggggtc taacataaat ggagatgcaa tttttaccag ttgagcttct ccaagtcggt
     1921 ataataatac aaatgcaaga gataatataa ttcctttttt attaaagaat gaagcaaagg
     1981 tagatataaa tccttctata ggatatttgt tgcctgcagc tgcattgtcg ttatttggtt
     2041 taggcagtat aaaataatgg taaattgcga tgcaaataaa aaatgccgca atgccataaa
     2101 atgtaacact ccaagctaag gcgatattat taaatttttg ctctaagtag cctgctaaaa
     2161 taaccaatag accttgtcca acaatttgtg cgattctata aaaagtactt cttataccaa
     2221 caaaaagcga ctgttgatgt tcgtctagag caagcatgta aaatccatcg gctgcaatat
     2281 catgtgtggc agaactaaag gcaattaacc acaaaacagc caatgtactt tgaaagaaga
     2341 atggggcagg tatacaaaat ccgactcctg ctaatgcaac tcccataata aattgcatac
     2401 ttacaaccca ccagcgtttt gtcttaaata aatcaacaaa agggctccaa aacggtttta
     2461 ttacccatgg taagtatagc caactggtat ataaagctgc gtcagcatta gaaatgccca
     2521 gctttttgta cataatcatt gaaaaagtgg ttgctgccac tacgtacgga attccctgac
     2581 caaaatataa tgatggtatc cacgcccaag gagatacttg tttttttgac atttaattga
     2641 cttttgattc gtcttattgt tcttgtgttt tcttttttga cttaatggct tctaatttta
     2701 cttttaattt gcgccaaaaa tccggagatt gagaactatt gttgtttgct tgatttaacc
     2761 ctgattctgc taatgc
//
LOCUS       DAOPID010000202         2770 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_51117_length_2770_cov_3.363904, whole genome shotgun sequence.
ACCESSION   DAOPID010000202 DAOPID010000000
VERSION     DAOPID010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51117_length_2770_cov_3.363904"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="PK641_06355"
     CDS             complement(<1..916)
                     /locus_tag="PK641_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG56000.1"
                     /translation="MKRLLIVALCILFHANVFAAWNIPVENYKQKDYNAGTQNWQIKQ
                     QKNQWMYFANNYGLLEFDGQEWQLYGIWSSSVVRSVEIVPEDSAIYVGGTNEYGIFKP
                     TSTGKLDFKPMSRNIPDEYKNFGQAWNLHWQNPYLYVQTDKYIFRQNKNGDFSVINPN
                     AHIFCSAMIRDALFVATDQGIYLITGTQLNALQGSELLHGKVIKAIREYSSNKILIAT
                     DFNGIYVFDGDAIKPFKTSVDNFIINNQLMDMVVNDNHIAFGTILNGVAVTNLDGNDA
                     IYINTENGLQTNTVLSMSFDKSGNLWLGL"
     gene            1161..>2770
                     /locus_tag="PK641_06360"
     CDS             1161..>2770
                     /locus_tag="PK641_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007156 - homophilic cell adhesion via
                     plasma membrane adhesion molecules [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin repeat domain-containing protein"
                     /protein_id="HPG56001.1"
                     /translation="MKTKHLLLSLSALILGSFAAQAVVPIGSAYGSDLMLYDQHNTES
                     VDPLTVKRNGDDFITLSYKIVSKGNKVWACTNLANAQIGGASWASQLRYWSATNQKTE
                     NNLVGRVTGTAVTYGSSANSYPDPDSFLMTFFQAAEPGGFAETECFAYTMGVNNNANP
                     AEVLAPQITECVVQNIEETSANLAISVSKGDCAEYYYEVTDAEHGIDEIFFFDNVVLN
                     DLISSTEYNLTITPRDFSGNAGTPYEVNFTTGGFIQISYGVANDIRFVLKSTESTLEV
                     YMEPTDTNKTFIGAAAYLTPAGSGSFEPAGKISPDGKYFYVKTSDNSLNGKIITIDCR
                     YNIDGEWVMSNNVITEGEMSGKPIKHLMGGAVAPETELPVLNGVEFIGRSDNSIILDL
                     DGQDNTIVYYEITGGEEPVNAFRTGQYYFTSFKPGNVYNLTITAMDLAGNRSSNSIQV
                     PFKAASRSNVFDGDNFNYNTTLKPEIPGGELAAIIQYNGETLTLGCTTISDKLPTGNQ
                     NRTFWSEQKSAFLPTVKIDGVSYSLESNV"
BASE COUNT          917 a          443 c          499 g          911 t
ORIGIN      
        1 ccagacccaa ccacaaattt cctgacttat caaaactcat acttaaaact gtattggttt
       61 gtagtccatt ttctgtatta atataaatag catcattacc atcaagattg gttacagcaa
      121 caccattcaa aattgtacca aaggctatgt gattgtcatt tacgaccata tccatcaatt
      181 gattattaat tataaaatta tcaactgagg ttttaaatgg tttaatagca tcgccatcaa
      241 aaacatatat accattaaaa tcggtagcga ttaaaatttt atttgatgaa tattcgcgaa
      301 tggctttaat aactttacca tgcaatagtt ctgaaccttg taaagcattt aattgcgtgc
      361 cggtaattaa atatatacct tggtctgtgg caacaaataa agcatcacga atcattgcag
      421 aacaaaatat gtgtgcattc gggtttataa cagaaaaatc cccattttta ttttgtctaa
      481 aaatgtattt gtctgtttgt acatataaat atgggttttg ccaatgcaga ttccatgctt
      541 gtccgaaatt tttatattca tcaggaatat tacgagacat aggtttgaag tcaagtttgc
      601 ctgttgaggt aggtttgaag ataccatact catttgtgcc accaacgtag attgcactat
      661 cttctggtac aatttctact gatcttacta cagaagaact ccatattcca tatagttgcc
      721 attcttgacc gtcaaattca agtaaaccgt aattgttggc aaaatacatc cattggtttt
      781 tttgttgttt aatttgccag ttctgggtgc ctgcgttgta gtctttttgt ttgtagtttt
      841 caacaggaat attccatgca gcgaaaacgt tcgcgtgaaa tagaatacac aatgcaacga
      901 ttaataatct tttcatacgt tttgattgat tggttaactg caaagttata gcatttattc
      961 taaattaata acattaatat gaaaagatta ctgcgtaagt attatagtta atagtttgtt
     1021 tattaatata ttaatatttt ttttgagaat atttgtcgta ttagataaag tgtaatgtcg
     1081 taatattgta ggataataaa ttttgatttc cttgaaacaa cttgcatctt tgctgtataa
     1141 accatttaaa cacatactat atgaaaacaa aacatttact cttaagcttg tcagcactta
     1201 tattaggaag ctttgctgcg caagctgttg tcccaatagg cagtgcctat ggcagcgatt
     1261 taatgttata tgatcaacat aatactgaat cagttgatcc tttaacagtg aaaagaaatg
     1321 gtgatgattt cattacctta tcttataaga tagtttctaa gggaaacaaa gtatgggcat
     1381 gtaccaattt ggcaaatgct caaataggag gagctagttg ggcatcacaa ttacgttatt
     1441 ggtcagctac aaatcaaaaa acagaaaata atctcgtagg cagagttacc ggaacagctg
     1501 ttacgtacgg cagttcggca aactcgtatc ctgatcctga ttcattctta atgactttct
     1561 tccaagctgc tgaacctgga ggatttgcag aaacagaatg cttcgcttat acaatggggg
     1621 ttaataataa tgcaaatcct gcagaagttt tagctcctca aattacagaa tgtgttgtac
     1681 aaaatatcga agaaacatct gcaaaccttg caattagtgt ttcaaaaggc gattgtgctg
     1741 aatattatta cgaagttaca gatgctgaac acggaattga cgaaatattt ttctttgaca
     1801 atgtagtgtt gaatgattta ataagctcaa ctgagtataa tttaactata acaccacgcg
     1861 attttagtgg taatgccgga actccttacg aagtaaactt tacaacaggc ggtttcattc
     1921 aaatatctta tggtgtagca aatgatatac gctttgtgct taagagcacc gaaagcacat
     1981 tagaagttta tatggagcca acagatacta ataaaacttt tattggagct gcagcttatc
     2041 taacacctgc aggaagtggt agttttgaac ctgccggaaa aattagtcct gatggaaagt
     2101 acttctatgt taaaacatct gataattctt tgaatggtaa aattataact attgattgca
     2161 gatataatat agatggtgaa tgggtaatgt ctaataatgt aattacagaa ggtgaaatgt
     2221 cgggtaaacc aattaagcat ttaatgggtg gtgcagtggc tcctgaaaca gaacttcccg
     2281 tattaaatgg tgtagaattt attggtagaa gtgataatag tataatattg gatttggacg
     2341 gtcaagataa tactatcgtt tactatgaga ttacaggcgg cgaagaacct gttaatgcat
     2401 tccgtacggg tcaatattac tttacgtcat ttaaaccggg taatgtgtat aacttaacta
     2461 ttactgcaat ggatttagca ggcaatcgtt caagtaattc aattcaagtg ccatttaagg
     2521 ctgcttcccg ttctaatgtt tttgatggcg acaattttaa ttacaatact accctaaaac
     2581 cggaaattcc tggtggtgag ttggctgcaa ttattcaata taacggagaa acacttactc
     2641 ttggatgtac aacaataagt gataaattac cgacaggaaa ccaaaataga acattttggt
     2701 cagaacaaaa gtcggcattt cttcccactg ttaaaataga tggagtgtca tattcattgg
     2761 aaagtaatgt
//
LOCUS       DAOPID010000203         2764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_51275_length_2764_cov_3.332964, whole genome shotgun sequence.
ACCESSION   DAOPID010000203 DAOPID010000000
VERSION     DAOPID010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51275_length_2764_cov_3.332964"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="PK641_06365"
     CDS             complement(<1..1044)
                     /locus_tag="PK641_06365"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPG56002.1"
                     /translation="MSIYKTAINKPVTTILIFVAVIIIGIFSFLKLPIDQFPQMDPPY
                     VMVLTTYAGANASEIETNVSKLLENSLNSVDGLKEITSTSKDNISVVTMEFEWGINMD
                     EVVNDVRSYIDMVKDNLPDGTSIPFIFKFSSSAMPIAQYAITADETYSGLDKLLNDEV
                     VPQLNRVDGIGNISLSGSPERYVYVDIDQEKLDAYRIPIETVGAAVSNNNLNLSSGSV
                     KMNKEQYQLQVRSEYVESSEINDLVVTTTLQGKQVYIRDIATVRDTIKDLTMEEKING
                     RDGVRMVIMKQSGANTVQICNDVRKEMAKIQKTLPSDVHIDIIYDSSENIQNSINSLQ
                     ESILYALLFVVLVI"
     gene            complement(1072..2103)
                     /locus_tag="PK641_06370"
     CDS             complement(1072..2103)
                     /locus_tag="PK641_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPG56003.1"
                     /translation="MKKFSILTVAAIVTLSFTACDGNKKEEKTEERVERVQTQILQQK
                     EISREINLATTLQGYETMNIASSISGIIEHIYVEVGDKVSQGQLLVRMDPTQYTTTKL
                     AYANLGVEMGRMEALKQAGSISQQNYDKIKVQYDQTKETLALYETNTFVKAQYSGYIS
                     AKNFENGELCAGQPILTLTQINFLKALINIPEAYFPFVKVGMPISIVSDIYPNKKFNG
                     TIEIVYPTIDINSHTFTIKVKIPNAQELLRPGMYVHTTIPVGKAKTLIAPYQAVLKLI
                     GSNDRYVFLNNNGRAKRVAVTLGQRFDENVEILSDEIKEGDELVVTGQARLIDGVKLN
                     VLNDSKSQQ"
     gene            complement(2205..>2764)
                     /locus_tag="PK641_06375"
     CDS             complement(2205..>2764)
                     /locus_tag="PK641_06375"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPG56004.1"
                     /translation="SSDLILDNNYDYQLVSKNTELAKKQITLTAMDYVPSLSGYYQYS
                     AKTYFGAAEGMNTTPPNMVGVSLGIPIWSSGKRASAVTEKKLAYAAAQNTLADTEDLL
                     KVKDKQLKYNLKSAYETYEVQKKNINVTQSVFENVSKKFEFGYASSTDVTTATQELLT
                     AQTNYISSMLEMVNAYIDLKNLLNK"
BASE COUNT          854 a          481 c          391 g         1038 t
ORIGIN      
        1 tattactaaa actacgaaaa gtaatgcata taatattgat tcttgtaaag aattaattga
       61 gttctgaata ttttctgatg aatcgtatat aatatctata tgcacatctg atggcaatgt
      121 tttttgtatt ttagccatct cttttctaac atcattacat atttgaacag tatttgcccc
      181 tgattgtttc ataataacca tacgaacacc atcgcgacca ttaatttttt cttccatggt
      241 aagatcctta atggtatcac gtactgttgc aatatctcta atgtaaactt gttttccttg
      301 taatgttgtt gttacaacaa gatcgtttat ctcagaactt tcgacatatt cactacgcac
      361 ctgtaattga tactgttcct tattcatctt aacactaccc gaagataagt tcaagttgtt
      421 attgctaaca gcagcaccaa cagtttctat aggaatacga tatgcatcca atttttcttg
      481 atcaatatct acatatacgt aacgttcggg agatcctgac aaagatatat tacctatacc
      541 atcaacacga ttcaattgag ggacaacttc atcattcaac aatttatcca atcccgaata
      601 agtttcatct gcagtaattg catattgagc aatgggcatt gcactggaac taaatttaaa
      661 gataaatgga attgatgtcc catcaggtag gttgtctttt accatatcta tatacgatcg
      721 gacatcattt actacttcat ccatatttat accccactca aactccattg taacaacaga
      781 aatgttatcc ttagatgttg atgtgatttc ttttaatcca tcaacagagt ttaaactatt
      841 ttctagaagt ttacttacat ttgtttcaat ttcactggca ttggctcctg cgtatgttgt
      901 taataccata acataaggag gatccatttg aggaaattga tcaataggta atttcaaaaa
      961 ggagaatata cctattatta taacagcaac gaaaataaga atggtcgtta ccggtttatt
     1021 aatggctgtc ttgtaaatac tcataattat atgactttat tcattattct attattgttg
     1081 tgatttagag tcatttaaga cattcaattt cacaccatca ataaggcgag cttgccctgt
     1141 aactactaat tcatcacctt ctttaatctc atcgcttagt atctcaacat tttcatcaaa
     1201 acgttgaccc aatgttacag caactctttt tgctctgcca ttattattta aaaatacata
     1261 acgatcattt gaaccgatta attttaaaac agcttgataa ggagcaatta aagtttttgc
     1321 tttaccaacc ggtattgttg tatgtacata catacccgga cgcaacaatt cttgtgcatt
     1381 cggtattttt acctttattg taaatgtatg agaattaata tctatggtag gatacacaat
     1441 ttcaattgta ccattaaatt ttttattagg ataaatatca gaaactatag aaataggcat
     1501 tccgactttt acaaatggaa agtatgcttc aggtatattt attaaagctt ttaaaaaatt
     1561 aatttgcgta agagtaagaa tcggttgtcc tgcacacaat tcaccatttt caaaattttt
     1621 tgcagatata tatcccgaat attgtgcttt tacaaaagtg tttgtttcgt ataaagccaa
     1681 ggtttctttt gtttgatcgt attgaacttt tattttatca taattttgtt gcgaaatact
     1741 tcctgcctgt ttcaatgctt ccatacgtcc catttctaca cctagatttg cataagctaa
     1801 ttttgtagtt gtatattgag taggatccat gcgaactaat aattgtcctt gcgatacttt
     1861 atcacctacc tcgacatata tatgttcaat aattccgctt attgaagacg caatattcat
     1921 ggtttcgtag ccttgcaatg ttgttgcaag attaatctca cgagaaatct ctttttgttg
     1981 aagaatttgt gtttgaaccc tctcaacacg ttcttcggtt ttttcttctt ttttgtttcc
     2041 atcacaagct gtgaaagaaa gtgttactat ggctgcaact gtcagaatac taaacttttt
     2101 catttttgta tattattata tttgttattt ttttgttttg tccaagaact tccatactaa
     2161 agaaacttaa aaggtatttt gtatcttaaa attaatttga ggaattattt attcaacaag
     2221 ttttttaaat caatgtatgc gttaaccatt tccaacatag aagaaatata attagtttga
     2281 gccgttaata attcttgagt ggccgtagta acatcagtac ttgaggcata tccgaattca
     2341 aattttttag aaacattttc aaatacactt tgtgttacat taatattctt tttttgaact
     2401 tcgtatgttt cgtaagcaga tttaagatta tacttcaatt gtttatcttt aacttttaat
     2461 aaatcttcag tatcagccaa agtgttctga gcagccgcat atgccaattt cttttcagtt
     2521 acggcagatg ccctttttcc gcttgaccat attggaatac ctaatgaaac tcctaccata
     2581 ttagggggag ttgtattcat accttctgct gccccaaaat atgttttagc agaatattga
     2641 taatatccac ttaaacttgg aacataatcc atagctgtaa gtgttatctg ttttttcgct
     2701 aattctgtat ttttacttac caattgataa tcgtaattat tatcaaggat taagtctgac
     2761 gaaa
//
LOCUS       DAOPID010000204         2730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_52129_length_2730_cov_5.157757, whole genome shotgun sequence.
ACCESSION   DAOPID010000204 DAOPID010000000
VERSION     DAOPID010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52129_length_2730_cov_5.157757"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1835
                     /gene="dnaE"
                     /locus_tag="PK641_06380"
     CDS             <1..1835
                     /gene="dnaE"
                     /locus_tag="PK641_06380"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPG56005.1"
                     /translation="IKKSRGIDLNIDAIPIDDPETYKLYSEGKTIGTFQFESAGMQKY
                     LRELKPTTFEDLIAMNALYRPGPMDYIPQFIARKHGREPIVYDIPITERYLKDTYGVT
                     VYQEQVMLLSRLLAGFTRGESDVLRKAMGKKLIAVLNNLKPKFIEGGHKNGYDPNVLE
                     KIWADWEKFASYAFNKSHATCYSWIAYQTAYLKAHYPAEFMAANLTRNKDEITEVTKF
                     MEECKSMGIKVLNPDVNESSLHFISNADGDIRFGLGGIKGVGEGAVEAIVKEREKNGR
                     YKSTFDFFERIDLSACNKKAMESLTLSGAFDSFGDVSREQLMEANSKGETVLEQLMRY
                     ATKYQADNVMQGSLFGIEEIKIQRPILPTVQPWSTLERLSKEKSLIGVYLSAHPLDDY
                     GIVINCLCNETMNELGNIDNVIERHDLNSQIVVAGIVTKVDRRISRANRPYILMTVED
                     YSGSHEFSLYGKNVEQFSSFAELNRYLMLDIIVQEKGADYNKWKKEPDNTNKLKEYEY
                     KITAINNLADIKKSAIKSLTLELQLENVDAGLISDLSSYLVSNPKDEDPDELITDLMI
                     NVRDLASNMVINCKSKQKIKVNMNLWNLLVTMKNNSVLSYFVNK"
     gene            1910..2224
                     /gene="trxA"
                     /locus_tag="PK641_06385"
     CDS             1910..2224
                     /gene="trxA"
                     /locus_tag="PK641_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPG56006.1"
                     /translation="MAEQFTDANFSEKLNSFPVVVVDFWAEWCGPCRMVGPIIDELAK
                     DYAGKALIGKVDVDNNDEVSMKYGIRNIPTILFFKNGELVDKQVGAASKSAFEQKINA
                     LL"
     gene            complement(2320..2568)
                     /locus_tag="PK641_06390"
     CDS             complement(2320..2568)
                     /locus_tag="PK641_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HPG56007.1"
                     /translation="MKQGIHPESYRPVVFKDMSNGDMFLSRSTAASKETIEFEGATYP
                     LIKLDISSSSHPFYTGKSTLVDTAGRVDKFNSRYKRNK"
BASE COUNT          955 a          355 c          549 g          871 t
ORIGIN      
        1 acataaagaa atcaagagga attgatttaa atatagatgc aataccaata gatgatcccg
       61 aaacctataa gttgtatagc gaaggcaaaa ctatcggaac attccaattt gagtctgccg
      121 gtatgcaaaa gtatctgcga gaactaaagc cgactacttt cgaagatttg atagccatga
      181 acgctttgta tcgtccgggt ccaatggatt acatccctca atttattgca agaaaacatg
      241 gaagggagcc tattgtttac gatatcccta taacagaacg ttatttaaaa gatacatatg
      301 gtgttactgt gtatcaggaa caagtcatgc ttctaagtag attattagca ggttttacca
      361 gaggtgagtc tgatgtatta cgtaaggcca tgggtaagaa attaatagct gttttgaata
      421 atcttaagcc aaaatttata gaagggggtc ataaaaatgg ttatgatcct aatgtattgg
      481 aaaaaatatg ggcagattgg gaaaaattcg catcatacgc atttaataaa tcacatgcta
      541 catgctattc gtggattgca tatcaaacag cgtatttaaa agctcattat ccggctgaat
      601 ttatggcagc taatcttaca cgtaataaag atgaaataac tgaggttacc aaatttatgg
      661 aagagtgtaa atctatgggt attaaggtgc taaatcctga tgtaaatgaa agtagtttac
      721 actttatttc taatgcagat ggtgatattc gttttggttt aggtggaatt aagggagttg
      781 gtgaaggtgc agttgaagcc attgtaaagg aaagagaaaa aaatggacgt tataagagta
      841 cttttgattt ctttgaacgt attgatttgt ccgcatgtaa taagaaagca atggagagtt
      901 taactttgag tggtgctttc gatagttttg gagatgttag tagagagcaa cttatggagg
      961 caaattcgaa gggcgaaact gtactcgaac aattaatgcg gtatgctact aagtatcagg
     1021 cagataatgt catgcaagga tcgctttttg gaatagaaga gataaaaata caacgtccta
     1081 ttttaccaac tgttcagcct tggtctacgc tcgaacgttt atcaaaggag aaatctttaa
     1141 taggagttta cttgtcagcg catccacttg atgattatgg tattgtaatt aattgtctgt
     1201 gcaatgaaac tatgaacgaa cttggtaata ttgataatgt tattgagaga catgacctta
     1261 attcgcagat tgttgttgcc ggcattgtta ctaaagttga tagacgtata tctcgtgcaa
     1321 atagaccata tattttgatg acagttgaag attattcagg atctcacgaa ttctccttat
     1381 atggtaaaaa tgtagaacaa ttttcgtctt tcgctgaact taatcgttat ctgatgttag
     1441 atataatagt acaggagaag ggtgcagatt ataataaatg gaaaaaagaa ccggataata
     1501 caaataaact gaaagagtat gaatataaaa tcactgcaat taataatttg gctgatatta
     1561 aaaaatcagc aatcaagtct ttaactttag agttgcaatt agaaaatgta gatgccggtt
     1621 taataagcga tttatcttca tatttggttt caaatccaaa agatgaagat cccgatgaat
     1681 taataacaga tttaatgata aatgtgcgtg atttagcgtc taatatggta attaattgta
     1741 aatccaaaca aaaaattaag gttaatatga atttatggaa tttattagta acaatgaaaa
     1801 ataattctgt tttatcctac tttgttaata aataaagttg gaatcatggc atttacttat
     1861 tatttttgta aaattatatg gttgaattta atttaataaa taaagaatta tggcagagca
     1921 atttactgat gcaaattttt cagagaagct aaatagcttc ccggttgttg tagttgattt
     1981 ttgggcagaa tggtgtgggc catgtagaat ggttggtcca attattgatg agcttgctaa
     2041 agattacgca ggtaaggctt taattggtaa agttgatgtt gataataatg atgaggtttc
     2101 aatgaaatac ggaattcgga atatcccgac tattttgttt ttcaagaatg gcgagttggt
     2161 tgataaacaa gttggagccg cttctaaaag tgcttttgag caaaaaatta atgctctact
     2221 ttaaacatcg aagtgatgaa ataaaaaaaa tcctccagaa atggaggatt tttttatgcg
     2281 ttaaatttaa atctacaaca caatataggc gaagtaaatt tatttatttc ttttataacg
     2341 agagttgaat ttatctacac gacctgctgt gtcgactaat gtcgatttgc ctgtgtagaa
     2401 tggatgtgaa ctactagaaa tatccaattt aattaaagga taagttgcgc cttcaaactc
     2461 aatggtttct ttgctggccg ctgtagagcg ggataaaaac atatcgccgt tagacatgtc
     2521 cttaaacacg acgggacgat aactttcagg atgaattcct tgtttcatat ttgtgataat
     2581 aattaattaa tttcggtaca aatcagggtg caaagataag tataagaact taatttacaa
     2641 aataactgat attatttttt gcaagttgtg agaaatgaaa attcaaataa gaagtgcgaa
     2701 acgcaaaaag cataaaagaa ttgaatttga
//
LOCUS       DAOPID010000205         2683 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_53353_length_2683_cov_5.552131, whole genome shotgun sequence.
ACCESSION   DAOPID010000205 DAOPID010000000
VERSION     DAOPID010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53353_length_2683_cov_5.552131"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /gene="ribD"
                     /locus_tag="PK641_06395"
     CDS             complement(<1..873)
                     /gene="ribD"
                     /locus_tag="PK641_06395"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HPG56008.1"
                     /translation="MYLENEKKYMRRCFQLSKLGLGKVAPNPMVGCVIVHNDKIIGEG
                     FHRQFGEPHAEINALNSVANKSLLKDSVLYVNLEPCSHYGKTPPCALRLINEGIKNVV
                     VCNLDPNPKVAGRGVTMLRDAGIEVSVGLLEQEGWVLNRRFFTYHTKHRPYIILKWAQ
                     TTDGYIDGIDEKPLLISSNITKALVHQMRAEEMAILVGTKTALKDNPKLHTRRWFGKN
                     PLRIAVDRNLSIPNYYNLYDGSVSTIIYNSVKSDNDCLVKLDFKSDIITQILADLYER
                     GIQSLIVEGGKFTLD"
     gene            893..1741
                     /gene="prmC"
                     /locus_tag="PK641_06400"
     CDS             893..1741
                     /gene="prmC"
                     /locus_tag="PK641_06400"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HPG56009.1"
                     /translation="MENLKTYFINTLKSIYSPNEIGILYRTSMEYVCCIPKQETYFRK
                     DTDFNDKQAAEMKSIAYRLQKNEPIEYILGETEFLSLKFKLNNKVLIPRPETEELVDW
                     IIKDVDNNKKLSIIDIGTGSGCIAVAIAKWVSNATVTALDISEDALQIAKKNAELNNV
                     QIQALCADILNISNTLQNKYDIIVSNPPYIRECEKTDMEANVLKYEPHTALFVSDENP
                     LIFYDGIAKFASSYLNENGLIYVEINRYLGSETLKMFESYNFNCIMKNDLSGANRMIK
                     AIKRKK"
     gene            1820..>2683
                     /locus_tag="PK641_06405"
     CDS             1820..>2683
                     /locus_tag="PK641_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme N-terminal
                     domain-containing protein"
                     /protein_id="HPG56010.1"
                     /translation="MNYLNFDKTVLSNLEKSLTKEMLRTNRSGAYNSTTIVDCNTRKY
                     HGQLVVPLPDIDKNNHVLLSSLDETIIQHKAEFNLGIHEYSGQHFSPNGHKYIREFSC
                     ELISKTVYRVGGVIISKERIMVSFEPRIITKYTLIEAHSPTIIRFKPFLAFRSVNKLS
                     KKNNAANTNYEDVNSGIKCQLYPQYPHLFMQFSKKAEYIHQPYWYENLSYNKEQERGY
                     EYEEDLVVPGYFEMPIKKGESIFFTAGLSEASPKKLKTIWEEELAKRNPRTDMFNCLK
                     NAAQQFYKKQED"
BASE COUNT          958 a          462 c          426 g          837 t
ORIGIN      
        1 atccaaagta aattttccgc cctcaactat taatgactga attcctcttt cgtataaatc
       61 ggccaatatt tgagtaataa tatctgactt aaaatctagt tttactaaac aatcattatc
      121 gctcttaacc gaattgtata taatagttga aaccgagcca tcatataaat tataataatt
      181 tggaatactc aagtttctgt caactgctat tcttaaagga ttcttcccga accaacggcg
      241 tgtatgaagt ttagggttgt cctttaaagc tgttttagtg ccaactaaaa ttgccatttc
      301 ctcagctctc atttgatgaa ctaaagcctt tgttatattt gatgatataa gtaaaggttt
      361 ttcatcgatt ccatcgatat aaccatcagt ggtttgagcc cattttaaaa taatatacgg
      421 acgatgtttt gtgtgataag tgaagaatct tcggtttaat acccaacctt cttgttctag
      481 aagtcctaca gaaacttcaa tacccgcatc acgcaacata gtaacacctc tgccggctac
      541 ctttggattc ggatctaagt tgcaaaccac aacatttttt attccttcat ttattaatct
      601 caaggcgcat ggaggagttt taccataatg agaacaaggt tcaagattta catacaaaac
      661 cgagtccttg agtaaagact tgttagcaac agaatttaaa gcatttattt cagcatgggg
      721 ttcaccaaat tgcctatgaa agccttctcc tataattttg tcgttatgta caataacaca
      781 accaaccatt ggattaggag ctacctttcc aagtccaagt ttggataatt ggaaacatct
      841 tcgcatatat tttttttcat tttccaaata catttttttg tagctttgcg atatggaaaa
      901 cctaaaaacc tattttatta atactctaaa gagtatttac agccctaacg agattggtat
      961 cctttacaga acaagtatgg agtatgtttg ctgcatacca aaacaagaaa cctactttcg
     1021 caaagataca gattttaacg ataaacaagc agcagaaatg aaaagtattg cttatcgttt
     1081 gcaaaaaaat gaacctatcg aatatatttt gggcgaaact gagtttctaa gtcttaaatt
     1141 taaactcaac aataaggttc taattcctcg acccgaaacc gaagaacttg ttgattggat
     1201 tattaaagat gttgataaca ataaaaaact tagcattatt gacataggaa ccgggagcgg
     1261 atgtatagct gtagctatag ccaagtgggt ttctaatgca acagttacag cccttgatat
     1321 ttctgaagat gcattgcaaa ttgcaaaaaa aaatgcagaa ttgaataatg tacaaataca
     1381 agctttatgt gctgatattt tgaacatatc aaatacatta caaaacaaat atgatataat
     1441 agtaagtaac ccgccttaca ttcgcgaatg tgaaaaaact gatatggaag caaatgtatt
     1501 gaaatatgaa cctcacacag cattgtttgt atcagacgaa aatcctttaa ttttttacga
     1561 tggaatcgca aaatttgcta gttcgtattt aaatgaaaat ggtttaatat atgtagaaat
     1621 taatcgctac ttaggtagtg aaactttaaa aatgtttgaa tcatacaatt ttaattgcat
     1681 aatgaaaaat gatttatcag gcgcaaaccg catgataaaa gcaattaaaa gaaaaaaatg
     1741 acgtatattt tgtcttataa atgctttgat ttgtggtttt tttatactat ttttgcaaac
     1801 gattgcaaat tttcaacata tgaattacct gaattttgac aagacagtac tctccaattt
     1861 ggaaaaatct ttaacaaaag agatgttacg caccaatcgt tcaggtgcat acaacagcac
     1921 tactattgtt gattgtaaca cgcgtaaata tcacggacaa ttagtagttc cacttcctga
     1981 tatagacaaa aataatcacg ttttgctatc ttcgttagat gaaacaataa ttcagcataa
     2041 agctgaattt aacttgggca tacacgaata ttcaggccaa catttcagtc caaacgggca
     2101 caaatacata cgtgaattta gttgtgaact tatttcaaaa actgtttaca gagtgggtgg
     2161 tgttattata tctaaagaaa gaattatggt ttcttttgag ccgcgcatca tcacaaaata
     2221 cactcttatc gaagcacact ctcctacaat aatcagattt aaaccatttc ttgcttttag
     2281 aagtgtaaac aaactttcga agaagaataa tgctgcaaat acaaattatg aagatgtgaa
     2341 tagtggaatt aaatgccaat tgtacccgca atatcctcat ttgtttatgc aattttcgaa
     2401 aaaagcagaa tatattcatc aaccatattg gtacgaaaat ttatcatata acaaagagca
     2461 agaaagagga tacgaatacg aagaagatct tgttgttccc ggatatttcg agatgccaat
     2521 aaaaaaaggc gaatcaattt tctttactgc aggcttatct gaagcatcac ctaaaaaact
     2581 aaaaactatt tgggaagaag aacttgcaaa aaggaacccg cgtaccgaca tgtttaactg
     2641 ccttaaaaac gctgcacaac agttttacaa gaaacaagag gat
//
LOCUS       DAOPID010000206         2682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_53381_length_2682_cov_4.083746, whole genome shotgun sequence.
ACCESSION   DAOPID010000206 DAOPID010000000
VERSION     DAOPID010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53381_length_2682_cov_4.083746"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="PK641_06410"
     CDS             complement(<1..438)
                     /locus_tag="PK641_06410"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HPG56011.1"
                     /translation="MNKSKINANHLFNSRITSIISISLVIFLIGVTTALLLMAGELSV
                     LVRENLTLSVVLKDNVKDADVQKLLKTLDASEYSKSTEYIDKEAASKELAEELGQDPV
                     EFLGYNPLLASVEMKLHSEYANNDSIARIETELRRMPEVKEVIY"
     gene            complement(431..>2682)
                     /locus_tag="PK641_06415"
     CDS             complement(431..>2682)
                     /locus_tag="PK641_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG56012.1"
                     /translation="ASPYLSMGGAIAFAFGSYNLIIIVAGHNTKAVAIAYMATIIASV
                     YVAFKKNPWLGSAMLALFLGLELYANHVQITYYTLIMIICFGVSELIFAIKEKTLPKF
                     FKNIGILLIGAVLAVGMNATRLITTAEYVKATMRGESNGLTVKGSDSHGLDKDYITQW
                     SYGINESMTLLIPNFMGGASSGTLDENSNTAQTISELTGYKKTGKELTKPERERLDYI
                     IQNNPDNFQARNLTELMYSFNLPLYWGTQPFTAGPVYVGAIICFLFVLGLLIVPARQR
                     WWLFAAAVIGLLLSWGKNFMPLTDFFIDYVPMYNKFRTVSMTLTITCLAMCIMAILAL
                     KEMLSDNLSKSVKNKSLYIAFGIVGGISLLLWMIPSLAGDFTSPNDQSFTDSYAFLQE
                     TLPLDRKEMLSSDAIRSFAFVFAAAVVLWISVKRKINTKWVVLVFAVLFTADMLPIAN
                     RYLNDSNFVSTRNLKNENKPSTADSYILDDKTLDYRVLDLTVDIFNSSQPSYFHKTVG
                     GYSAVKLRRYQELIDVQLAPEIMRLQKELSTIRSEQDADSVFVNMNILNMLNTRYLIY
                     HPSALPLLNKSAFGNAWLVDNIKFVDTPDDEISLLDKTNSKQTLLVDKQYASECPKTD
                     FKKGDAFIQLISYSPNELKYAFKSDTPQMAVFSEIFYYKGWQAYNGDKKLPHIRANYL
                     LRAMYLEPGSYDITFKFEPKSYDIGNILALISSILLTLAFAYIIFAKLKEKRSKTLVN
                     QVKNTSSNE"
BASE COUNT          870 a          482 c          437 g          893 t
ORIGIN      
        1 gtagataact tctttcactt caggcatacg acgaagttct gtttcaattc gagcaatact
       61 atcattattg gcatactccg aatgtaattt catttctacg gatgccaaca aaggattata
      121 tcctaaaaac tctacagggt cttggcctaa ctcttcagcc aattctttcg atgccgcctc
      181 tttatctata tactctgttg attttgaata ttccgatgca tctaatgttt taaggagctt
      241 ctgcacatca gcatctttaa cattgtcttt aagtacaacc gataatgtta ggttttctct
      301 aacaagtact gacaattcgc ctgccatcaa taataatgca gtagtaacac ctattaagaa
      361 aataaccaat gagatactga ttatagatgt tatacgtgaa ttaaaaagat gattcgcatt
      421 tattttagat ttattcattt gaactcgtgt ttttaacttg atttacaagt gtttttgaac
      481 gtttctcttt aagttttgca aagataatat atgcaaaagc caatgtcaat aggatagaac
      541 taattaatgc taaaatattt cctatatcgt atgacttcgg ttcaaattta aatgtaatat
      601 cgtatgaacc gggttcaaga tacattgcac gtaataagta gtttgctctt atatgtggca
      661 attttttatc tccattataa gcctgccaac ctttataata aaaaatttca gagaatacag
      721 ccatttgcgg tgtatcagac ttaaatgcat acttaagttc gttaggacta tatgaaatta
      781 attgtataaa tgcatctcct tttttaaaat cggttttagg gcactctgac gcatattgtt
      841 tatcaacaag cagcgtttgt tttgaattgg tcttgtccaa tagagagatt tcatcatcag
      901 gagtatcaac aaatttaata ttatcaacaa gccatgcatt tccaaaggct gatttattaa
      961 gtaatggcaa tgctgatgga tgataaataa gatatctggt attaagcata ttaagaatat
     1021 tcatattcac aaatacacta tcagcatctt gctcactacg tatagttgat aattcttttt
     1081 gcaaacgcat tatttccgga gctaattgta catctataag ttcttgataa cgccttaatt
     1141 ttacggcact atatccacca acggttttat ggaaatatga aggttgagaa ctgttgaaaa
     1201 tatctacagt taagtctaaa actctatagt ctaatgtttt atcatctaaa atatagctat
     1261 ctgctgtaga aggtttgttt tcgttcttca agtttcgtgt tgatacaaag ttcgaatcgt
     1321 ttaagtaacg gttagctatt gggagcatat cagccgtaaa taaaacagca aataccaata
     1381 caacccattt tgtattaatc ttgcgtttta cagaaatcca taaaactaca gcagcggcaa
     1441 atacaaaagc aaatgaacgt atggcatcag aactaagcat ttcttttctg tctaatggca
     1501 acgtttcttg caaaaatgca taactatcgg taaatgactg atcgttaggg cttgtgaaat
     1561 cgccggctaa agaaggtatc atccataata acaaagaaat tcctccgact attccgaagg
     1621 caatgtaaag actcttattt ttaactgatt tggataaatt atcagataac atttctttta
     1681 aggctaatat tgccatgata cacatcgcca aacaagttat ggtcaatgtc atagaaactg
     1741 ttctgaattt gttgtacatc ggtacataat ctataaaaaa gtctgtaagt ggcatgaaat
     1801 ttttacccca cgacaatagc aagcctataa ctgctgctgc aaaaagccac caacgctgac
     1861 gagcaggtac aataagtagt cctaatacaa acaagaaaca aataatagcg cctacatata
     1921 caggacctgc agtaaatggc tgcgttcccc aatacaaagg taaattaaag gagtacatca
     1981 actcggttaa gtttcttgct tggaaattat caggattatt ctgtatgata taatcaagtc
     2041 tttctctttc cggtttagtt aattctttac ctgttttctt gtatcctgta agttcagaaa
     2101 ttgtttgtgc tgtgtttgag ttttcatcta aagttccgga cgatgcccct cccataaagt
     2161 taggtataag caaggtcata gattcgttaa tgccgtaact ccattgagtt atatagtctt
     2221 tatccaaacc atgactatcc gaacctttaa ctgtaaggcc attactctcg ccacgcattg
     2281 ttgctttaac atactctgca gtagttatga gtcgagttgc attcatgcct acggcaagaa
     2341 cagctccaat aagtaaaatt cctatgttct tgaaaaattt gggtagggtt ttttctttta
     2401 tggcaaatat taattcggat actccgaaac atattatcat tattaaagtg taatatgtaa
     2461 tttgaacgtg atttgcatat agttctaaac ctaaaaacaa ggctaacata gcagagccta
     2521 gccatggatt tttcttgaaa gctacataaa cactggctat tatagtagcc atatatgcaa
     2581 ttgcaactgc ttttgtatta tgccctgcaa caataattat taaattatac gagccgaagg
     2641 cgaaagctat tgctcctccc attgacaaat atggacttgc ac
//
LOCUS       DAOPID010000207         2636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_54653_length_2636_cov_4.357613, whole genome shotgun sequence.
ACCESSION   DAOPID010000207 DAOPID010000000
VERSION     DAOPID010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54653_length_2636_cov_4.357613"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /locus_tag="PK641_06420"
     CDS             <1..1092
                     /locus_tag="PK641_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPG56013.1"
                     /translation="AVIIAGLSFYVTHNLVNSLSIEEKARMEIWANATRELVSMPDGC
                     DLTFVQEVISENTTIPVVLADTSFRVLSYRNLEKDDYTEAELTAIARSFAAARKSIVV
                     TMDDGEKQYICYGNSLLLKKLAYFPIVQFMVVALYLILVVLVLLAMKRAEQNKVWVGL
                     SKETAHQLGTPISSMLAWTEILKTKYPDDKMMFEMEKDVNRLRTIAERFSQIGSKTEF
                     ELVEINQLITKSLTYIRTRISKKVNIEFCSGSTYYVMINIPLFEWVIENLCKNAVDAM
                     NGEGGITIYIGDKESNVFIDITDTGKGISKKNIKKVFNPGFTTKKRGWGLGLSLAHRI
                     IEEYHKGKIYVKHSEINKGTTFRIELKKV"
     gene            1202..1714
                     /locus_tag="PK641_06425"
     CDS             1202..1714
                     /locus_tag="PK641_06425"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HPG56014.1"
                     /translation="MPIVSKYLIPLKSDDNLNATYSFVLNDNFFESLEDAEIKKGDVK
                     AYLKVKKLGEAFCIDVLMDGSVEVPCVRCLDPVTVEIKVDEHLKVSYGEAYLEEDDDR
                     VIIPEDSEGIDMEWLMYEFIVLSIPIQHKHKEGDCNKQMESLLSNYENQLNEQADPRW
                     DALKSLIKNN"
     gene            1727..1912
                     /gene="rpmF"
                     /locus_tag="PK641_06430"
     CDS             1727..1912
                     /gene="rpmF"
                     /locus_tag="PK641_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPG56015.1"
                     /translation="MAHPKRRQSKTRTAKRRTHDKAVAPTMATCPNCGALHISHTVCG
                     ECGYYRGKLAVEQTANA"
     gene            2287..>2636
                     /locus_tag="PK641_06435"
     CDS             2287..>2636
                     /locus_tag="PK641_06435"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259613.1"
                     /note="catalyzes the formation of L-tryptophan from indole
                     and L-serine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPG56016.1"
                     /translation="MKTTKKIVLSEDEMPKYWYNVLADMPNKPLPPLNPKTKQPVSLS
                     DLEPIFAKGLIAQEVSTERWVEIPEEVRNLYRIYRPTPLVRASGLEKALDTPAKIYFK
                     NESVSPVGSHKLNSA"
BASE COUNT          941 a          359 c          509 g          827 t
ORIGIN      
        1 gcagtgataa tagcaggcct gtctttttat gttacacata atttggtaaa tagtttgtct
       61 atagaagaga aggctagaat ggaaatatgg gcaaatgcaa ctcgcgaatt agtaagtatg
      121 cctgatggat gtgatttaac attcgttcaa gaggtaatat ccgaaaatac tacgattcct
      181 gttgttcttg cagacacttc atttagggtc ttgtcttata gaaatttaga aaaagatgat
      241 tatacagagg cagaattaac tgcaatagcg cgaagttttg ctgctgctcg taaatcaata
      301 gtagtaacaa tggatgatgg tgagaaacaa tatatctgtt atggtaattc tttattgctt
      361 aaaaagttgg catattttcc catagttcaa tttatggttg ttgcattgta tcttatactt
      421 gtagtattgg tgttgttagc aatgaagcgt gctgaacaaa ataaagtatg ggtaggttta
      481 tctaaagaaa cagctcacca gttgggtact ccaatttcgt caatgttagc ttggactgag
      541 atattaaaga ctaaatatcc tgatgataag atgatgttcg aaatggaaaa agatgtcaat
      601 cgtttaagaa ctattgccga gcgtttttca caaataggtt ctaaaacaga atttgaattg
      661 gttgaaataa atcaactcat aacaaaatca ctaacatata ttcgcacacg tatttcaaaa
      721 aaagtaaaca tagaattttg ttcagggagt acatattatg taatgattaa tattccatta
      781 ttcgaatggg ttatagaaaa tctatgtaaa aatgcagtag atgcaatgaa tggggaaggt
      841 ggcataacta tatatattgg agataaagaa tcgaatgtat tcatagatat tacagatacg
      901 gggaagggga tctctaaaaa aaatattaaa aaagtattta atcccggctt tactacaaaa
      961 aaacgtggct ggggcttagg tttgtcattg gcacatcgaa tcatagaaga gtatcataaa
     1021 ggaaaaatct acgtcaaaca ctctgaaatt aataaaggta caaccttcag aatcgaatta
     1081 aaaaaagtat gatgttgata aatctgcatt tgttttctaa tcactaaaaa tatcgtgtaa
     1141 aaagtttttt gattcgtgaa aaatacttac ctttgtcccc acttttatag aagtatttgg
     1201 tatgccgata gtttccaagt atctgattcc gctgaaaagc gatgataatc tgaatgcaac
     1261 atatagcttt gtgctaaatg ataatttctt tgagtcgtta gaagatgcag aaattaaaaa
     1321 aggtgacgta aaagcttatt tgaaagttaa aaaactaggt gaagcttttt gtatcgatgt
     1381 gttgatggat ggttctgttg aagttccttg tgttcgttgc ttagatcctg tgactgtgga
     1441 aataaaagta gatgaacact taaaggtaag ttatggtgag gcttatttag aggaagatga
     1501 tgatagagtt ataattcctg aagattctga gggtatagat atggaatggt tgatgtatga
     1561 attcatcgtt ttatcaatac cgatacagca taaacacaaa gaaggagatt gtaataaaca
     1621 aatggaaagt ttacttagca attacgaaaa tcaattaaac gaacaagcag atccccgttg
     1681 ggatgcttta aaatcattaa taaagaacaa ttaaatatag aaaattatgg cacatcctaa
     1741 aagaagacaa tcaaaaacaa gaacagcaaa acgtagaact catgataaag ccgttgctcc
     1801 tacaatggca acttgtccta attgcggtgc cttacatata tctcacacag tatgtggtga
     1861 atgtggctat tatcgtggaa aattggcagt agaacaaact gcaaacgcat aaatctgatt
     1921 actaagcaaa tacaaattat tttgtaattt tattagtaat tgttggtatt tttaaaaata
     1981 agtttgtacg tttgtgtcgt agtattgata catgataata taaagagttt ttataaaaat
     2041 gaaaaattta gcattaaaca ataattggtg gtggcaacgc tttatgaatt aaggtggatg
     2101 tgcgctcgtg ttgtttaatg aatctcataa agagtcgtag ggaatccacc tgcgactctt
     2161 tttttattaa ttataatgtc aaaatatgga aaaaattgtg tttcgatggc gtaagttgtg
     2221 ccgatattaa taaaaaggtg tttcttaaaa atttagtact caataataat tttaaatata
     2281 tttatcatga aaacaacaaa aaaaatagtc ctttcagaag acgagatgcc gaaatactgg
     2341 tataatgtgt tggccgatat gccaaataaa ccacttcctc ctcttaatcc aaaaaccaaa
     2401 caaccggttt ctctatcaga tttggaacct atatttgcaa aaggtcttat agcacaagaa
     2461 gtttcaacag aacgttgggt tgaaattcct gaagaggtgc gtaatttgta tcgtatatat
     2521 cgcccaacac cattggtacg tgcttccggt ttggaaaaag ccttagatac tcccgcaaag
     2581 atttatttta aaaacgaaag tgttagtccg gtaggttcgc acaaattgaa ttctgc
//
LOCUS       DAOPID010000208         2611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_55410_length_2611_cov_4.092332, whole genome shotgun sequence.
ACCESSION   DAOPID010000208 DAOPID010000000
VERSION     DAOPID010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55410_length_2611_cov_4.092332"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..1596
                     /gene="groL"
                     /locus_tag="PK641_06440"
     CDS             4..1596
                     /gene="groL"
                     /locus_tag="PK641_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941147.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPG56017.1"
                     /translation="MKKGVDELANAVKVTLGPKGRNVILAKKFGAPHITKDGVTVAKE
                     IELEDAFQNMGAQMLKEVASKTGENAGDGTTTATVLAQSIISVGLKNVAAGANPMDLK
                     RGIDKAVVKVVEHIKSQAEEVGDDIKKIEQVATISANNDSEIGKLIADAMAKVKKEGV
                     ITVEEAKGTDTTVDVVEGMQFDRGYLSPYFVTNADKMEAVMEKPYILIHDKKISSLKD
                     ILPLLEAVVQGSRPLLIIAEDVDSEALTALVVNRLRANLKICAVKAPGFGDRRKEMLQ
                     DIAILTGGTVISEEQGLKLETATMDMLGSADTVTINKDNTTIVNGAGDKKAIANRVAQ
                     IKVQIDTTTSQYDKEKLQERLAKLAGGVAVLYIGAPSEIEMKEKKDRVDDALSATRAA
                     VEEGIVAGGGVAYIRAISALNNLKGENEDETTGIEIIKRAIEEPLRQIVANAGKEGAV
                     IVQKVSEGKNDFGYNARTDTFENLFSTGVIDPAKVARVALENAASIAGMMLTTECVIA
                     DKKEDNPVPAMPPMGGGMGGMM"
     gene            1827..2552
                     /locus_tag="PK641_06445"
     CDS             1827..2552
                     /locus_tag="PK641_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HPG56018.1"
                     /translation="MNNSPLLRALIEQKESKLHGGIYHHTQINLTYNSNHIEGSRLTK
                     DQTRYIFETNTIDISKEAINVDDILETTNHFRCINYIIDHANDDLSENFIKRLHEMLK
                     SGTRDASKDCFAIGAYKRLPNEVGGMETSTPEDVEEHIQKLLKNYIEKKNPNFEDIID
                     FHQCFEAIHPFQDGNGRVGRLIMFKECLKNNIVPFIITDDLKMFYYRGLREWPNIKGF
                     LTDTCLTAQDQYKESLDYFRIEY"
BASE COUNT          911 a          453 c          525 g          722 t
ORIGIN      
        1 cgcttgaaaa aaggtgtcga cgaattggcg aatgccgtaa aagtaacact tggacctaaa
       61 ggacgcaatg tgattttagc taagaaattt ggagctcctc acatcacaaa agatggtgtt
      121 acagtagcaa aagaaatcga attggaagat gcattccaga atatgggtgc tcaaatgctt
      181 aaagaagtag cttcaaaaac aggcgaaaat gcaggtgacg gaacaactac tgctacagta
      241 ttggcacaat ctatcatttc tgtgggtctt aaaaacgtag ccgcaggtgc aaatccaatg
      301 gatttaaaaa gaggtataga taaagctgtt gtaaaagttg ttgagcacat taaatcacaa
      361 gctgaagaag ttggtgatga tatcaaaaaa attgagcaag ttgctacaat ttctgccaat
      421 aatgatagtg aaatcggtaa acttattgcc gatgcaatgg ctaaagttaa aaaagaaggc
      481 gttatcactg tagaagaagc aaaaggtaca gatactacag ttgatgttgt tgaaggtatg
      541 cagtttgacc gcggctattt atctccttat tttgtaacta atgcagataa gatggaagca
      601 gtaatggaga aaccatatat cttgattcat gacaaaaaga tttcttcatt aaaagatatt
      661 cttcctttgt tggaagctgt tgttcaaggc agccgtcctc ttttaattat tgctgaagac
      721 gttgatagtg aagcgttaac agctttagta gttaatcgtc tacgtgctaa tttgaagatt
      781 tgtgcagtta aagcaccggg atttggtgat cgtcgtaaag aaatgttgca agatatcgca
      841 atcctaacag gtggaacagt aattagcgaa gaacaaggtt tgaaacttga aacagctact
      901 atggatatgt taggttctgc tgacactgtt acaatcaaca aagacaatac tactatagta
      961 aacggagccg gtgacaaaaa agcaatcgca aaccgtgttg ctcaaatcaa ggttcaaatc
     1021 gatacaacaa catctcaata cgataaagaa aaattgcaag aacgtttggc taaattagcc
     1081 ggtggtgttg cagtccttta cattggcgct ccttctgaaa ttgaaatgaa agagaaaaaa
     1141 gaccgtgttg atgatgctct tagcgcaacc agagctgctg ttgaagaagg tatcgttgcc
     1201 ggtggcggtg ttgcatacat tcgtgcaata agtgctttaa ataacctaaa aggcgaaaac
     1261 gaagatgaaa caaccggtat agaaatcatt aaacgcgcta ttgaagaacc attacgtcaa
     1321 atcgttgcta atgcaggcaa agaaggtgct gtaattgttc agaaagttag cgaaggcaaa
     1381 aatgacttcg gatacaacgc acgtaccgat acttttgaaa acctatttag tacaggtgta
     1441 atagaccctg ctaaagtagc tcgtgtagct cttgaaaatg cagcttcaat tgcaggtatg
     1501 atgttaacta cagaatgcgt aattgcagat aagaaagaag acaatccggt acctgcaatg
     1561 cctcctatgg gtggcggcat gggtggaatg atgtaatctc ctactctatt ttataacaaa
     1621 agcctttgtt ctttatgagc aaaggctttt ttattataaa actccctact gatctacttt
     1681 cagcaaaaga aatcactaaa ttattctaag caaaaaatgc gaaggatcac ctcttttttc
     1741 tccgaaagga tattcaaaat tattttattg ctacggtaaa aaatgattat atttgtatct
     1801 gtaaaaattc ttgtcaataa acaattatga acaactctcc gcttcttcga gcattgatag
     1861 aacaaaaaga gtcaaagttg catggcggca tctaccacca tacacagata aaccttacct
     1921 ataactctaa ccatatcgaa ggtagtcggc taactaaaga tcagacacgt tatatttttg
     1981 aaaccaatac gatagacata agcaaagagg caatcaatgt tgatgatata cttgaaacaa
     2041 caaatcactt tcggtgtatc aactacatta ttgaccatgc aaacgatgat ttatctgaaa
     2101 attttataaa gcgtcttcat gaaatgctaa agtcaggcac gcgagatgct tcaaaagatt
     2161 gttttgccat aggtgcatat aaacgcttac ccaacgaagt cggaggtatg gagacttcta
     2221 caccggagga tgttgaggag catattcaga aattgttgaa aaattacata gaaaaaaaga
     2281 acccgaactt tgaagacatc attgattttc accagtgttt tgaagccatc catccttttc
     2341 aagacggcaa tggcagagtc ggtaggctca ttatgtttaa agaatgtctt aaaaacaaca
     2401 tagttccatt catcataacc gatgatctga aaatgtttta ttaccgtgga ttacgcgaat
     2461 ggccgaatat taagggtttt cttacagaca cttgccttac tgctcaagac caatacaagg
     2521 aatcactaga ttattttaga atagaatatt agtagcgcag aatcactgcc cttaaaaata
     2581 tctatcaaat acagcttttt cgttcgtcaa a
//
LOCUS       DAOPID010000209         2590 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_56010_length_2590_cov_3.606312, whole genome shotgun sequence.
ACCESSION   DAOPID010000209 DAOPID010000000
VERSION     DAOPID010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56010_length_2590_cov_3.606312"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2388)
                     /locus_tag="PK641_06450"
     CDS             complement(<1..2388)
                     /locus_tag="PK641_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HPG56019.1"
                     /translation="MKTSRFIHIITLVCLLIGSISVSATNVTLKVIDKTKGSYNNGSV
                     YAWIGQYDFSWEAIGNISNVNSWYADFSNNNTGSKITKTSDEWIWEFTFNAAAGNYSW
                     NPYMGDPNGTSIEICKTLNITSNNIDFFIDNNGSLSGETSVVIYSASAGAKGDIAFGN
                     NNITGSIDSNAPTDLNATVFGTSSNSFSLNLSASDDNSVYYTIRYRRIDETNIIPYYS
                     ALHVTGTSIYNAGNKEIKLRGTNLGSWLVQEDWMTGDICGDQRSTINTLYNRFGAQKA
                     EELIDLWEENWITEKDLDNIKNLGMNLVRVPFTWMNLMDMNTREWKPDAWERLDWILK
                     ECSKRDLWVILCMHGAPGSQNGRDHSGVSGGSDEQAASEFWFGANAPANQTLYYEIWE
                     TIAARYKNNATIAGFDLLNEPFCQYRYTSTLGGDVLHSILYSVYNNTYKKIRKIDPNR
                     ILIMEAVWDPWDLPNPSSYSWSNVIYEYHNYEYSDYDNYEGKQITSMTNKINSINGAK
                     NAYNLPSYMGEFCYFNTPATWETGMALLNNNDIHWTSWSYKVQAYYGNWGLYNCTKRN
                     INLSNASELEIRDAWSNSTVTKNTNIADIISYYASNGIDKSPTSGIYYNTWTENPSGY
                     TASTIITGLKPNATYEYIVNAYDKYGNMTSDLKATVKTLNTEEQDSYDYWVIYRDNVS
                     DMSNYLDLRSNSFADWAGTTSQIEADKYESENAMAYIVNSAKGSWFGFGIINISGTYN
                     FEELSNYKLHFAYKTNYNGPLYVKLGGTNGEYSVEFSPISDNKWHTQEIAMSSFTD"
BASE COUNT          768 a          498 c          380 g          944 t
ORIGIN      
        1 atcagtaaag ctactcattg ctatttcctg agtatgccac ttgttatccg aaatagggct
       61 gaactcaact gaatattctc cattagtacc tcctaatttt acatataaag gtccgttgta
      121 atttgttttg taagcaaagt gtaatttgta attgcttaat tcttcaaaat tatatgttcc
      181 gcttatattt attataccaa aaccgaacca actccctttt gcagagttaa ctatatatgc
      241 catagcattt tcactttcgt atttatctgc ctcgatttga cttgtagttc ctgcccaatc
      301 tgcaaaacta ttactgcgta aatccaaata atttgacata tcagaaacat tatctctata
      361 tattacccaa taatcgtaac tatcttgctc ttcagtattc aaagttttga cagtagcttt
      421 taaatcagaa gtcatattgc catatttgtc gtaagcattt acaatatatt cgtatgttgc
      481 atttggcttc aatcctgtaa taatagttga tgcagtgtat ccacttggat tttccgtcca
      541 tgtattgtaa tagattccgc ttgttggact tttatcaata ccattacttg cataatacga
      601 aattatatct gctatgttag tgttttttgt tactgtagaa tttgaccaag catcacgaat
      661 ttctaactcc gatgcatttg acaaatttat gttacgcttt gtgcaattat ataatcccca
      721 attaccatag tatgcttgaa ctttgtatga ccaacttgtc caatgtatat cgttattatt
      781 tagcaaagcc atacctgttt cccaagtggc aggagtgttg aaatagcaaa attcacccat
      841 ataacttggt aaattgtaag catttttagc accattaatg ctattaattt tatttgtcat
      901 gcttgtaatc tgtttacctt catagttgtc gtaatcgcta tattcataat tatgatattc
      961 gtatattacg tttgaccatg aataactcga cggattaggt aaatcccacg gatcccatac
     1021 tgcttccata ataagtatgc gattagggtc tatctttctg atttttttgt atgtattatt
     1081 gtaaacagag tatagtatgc tatgcaatac atcaccacct agtgtacttg tgtaacgata
     1141 ttggcaaaat ggttcgttaa gtaagtcgaa tccggctata gttgcattgt ttttataacg
     1201 agctgcaata gtttcccata tttcataata caaagtttga tttgcaggcg catttgctcc
     1261 aaaccaaaat tcgcttgcgg cctgttcgtc gctacctccg gatacgcccg agtggtctct
     1321 gccattttgc gaacctggag ctccatgcat acataaaata acccataaat cacgtttgga
     1381 acattctttt aatatccaat ctaatcgttc ccaagcatcc ggtttccatt ctcttgtatt
     1441 catatccata agattcatcc aagtgaaagg tacacgaact aaattcattc ctagattctt
     1501 aatattatct aagtcttttt cggtaatcca gttttcttcc cacaaatcaa taagttcttc
     1561 tgctttttgt gctccgaatc tattatacaa tgtgtttatt gtagaacgtt gatcaccaca
     1621 aatatctcct gtcatccaat cttcttgaac taaccaagaa cccaaattag tgccgcgtaa
     1681 tttaatttct ttatttccgg cattgtatat tgatgttccg gttacgtgta acgcgctgta
     1741 atatgggatt atatttgttt catcaattct gcgataacgt attgtgtaat aaaccgaatt
     1801 atcgtctgat gcagacaaat taagcgagaa actattactg cttgtgccaa aaactgttgc
     1861 atttaagtca gtaggggcat tagaatcaat acttccggtg atattgttat tgccgaaagc
     1921 aatatcgcct tttgctcctg ctgatgcaga atatattaca acagatgttt ctccactcaa
     1981 agaaccatta ttatcaataa agaaatcaat attattagat gtgatattca atgttttaca
     2041 aatttcaatg cttgtaccat tgggatctcc catatatgga ttccacgaat aattaccggc
     2101 tgctgcatta aaagtaaatt cccaaatcca ctcatcactt gtttttgtta ttttacttcc
     2161 ggtattatta ttactaaaat ctgcatacca actgtttaca ttggatatat tgccaattgc
     2221 ctcccaagaa aaatcatatt gacctatcca tgcatatacc gaaccattgt tataagaacc
     2281 ctttgtcttg tcaataactt ttaatgtaac gtttgttgca cttacactaa tactaccaat
     2341 tagcaggcat actagtgtaa taatgtgaat aaatcttgat gttttcattt taatatggtt
     2401 aatccgaatg gttatttact taataagaac tttaattaca ttgctatcaa tgcgtaataa
     2461 atataatccg gaaggcattg aatttatcga caaataactt gtaccatcta tagcctcagt
     2521 ctttatcata ctgattccat tcatatgtat tagttctatt tgtgaacctt ttgatgtgcc
     2581 attaatgcat
//
LOCUS       DAOPID010000210         2580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_56308_length_2580_cov_2.833267, whole genome shotgun sequence.
ACCESSION   DAOPID010000210 DAOPID010000000
VERSION     DAOPID010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56308_length_2580_cov_2.833267"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="PK641_06455"
     CDS             complement(<1..904)
                     /locus_tag="PK641_06455"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPG56020.1"
                     /translation="MKKLAIIGAGYLQEPLVLKAKEMGIYTICFAWKEGAVCAEVADK
                     FYPISIIEKEEILAICISEKIDGICTIASDVAAPTVAYVANNMGIIGNNYDAAVRANN
                     KALMREAFYKADVPCPWFQKITSYTDLKNKDLKFPLIVKPVDRSGSMGVSKVNNNAEL
                     RIAINQALSDSFLKQAMVEQFVDGREISVEFISYKGQHYPLAITDKVTTEAPHFVELE
                     HHQPSTLAADIQQNIFKITEKALNALGITNGASHTEYKITKDNDIYIIELGGRMGGDF
                     IGSDLVQLSTGYDFVKAVIDVALNQ"
     gene            complement(976..2283)
                     /locus_tag="PK641_06460"
     CDS             complement(976..2283)
                     /locus_tag="PK641_06460"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HPG56021.1"
                     /translation="MNIAIVGTGYVGLVSGACFAEMGTNVTCVDVDANKINALKQGEI
                     PIFEPGLEEIVLRNVSAGRLSFTTDLTEVLNTVEVVFSAVGTPPDEDGSADLKYVLEV
                     ARTVGKNMNKHILLVTKSTVPVGTSKKVRQAIQAELDKRNSKLTFDVASNPEFLKEGA
                     AIKDFMSPDRVVVGVESDTAKEIMSRLYKPFLLNNFRVIFMDIPSAEMTKYAANSMLA
                     TRISFMNDIANLCELVGADINNVRRGIGSDPRIGSKFLYAGCGYGGSCFPKDVKALIK
                     TAQQNGYNMQLLNAVEEVNDNQKSILYQKLANKFNNNLNDKAVAIWGLTFKPNTDDMR
                     EAPSVVLINKLLEAGCKLRVFDPVVKEINITDKNLTFASDIYDAALNVDAICLVTEWQ
                     EFRLPSWQVLLKTMRTPIIIDGRNIYDKQELENLGFYYEGIGV"
BASE COUNT          767 a          502 c          385 g          926 t
ORIGIN      
        1 attgattaag tgctacatca attacagctt ttacaaaatc gtatcctgta gataattgta
       61 caagatctga cccaataaaa tcaccaccca tacgtccacc taactctatg atataaatat
      121 cattatcttt agttatctta tattcggtat gtgatgcacc atttgttata cccaaagcat
      181 ttaatgcctt ttctgtgatt ttgaatatat tttgttgtat atcagcagct aaggtagatg
      241 gctgatgatg ctccaattca acaaaatgag gtgcttctgt tgtaacctta tctgtaattg
      301 ccaatggata atgttgccct ttataactga taaattcaac gctgatttct cggccatcaa
      361 caaactgctc taccattgct tgttttaaaa aagaatcaga aagggcttga ttaatagcta
      421 ttctaagttc ggcattatta ttaacctttg aaactcccat actgccggaa cgatcaacag
      481 gtttaacaat taaaggaaac tttaaatctt tgttttttaa atcggtataa gatgtaatct
      541 tttgaaacca aggacatgga acatcagcct tataaaaagc ttcgcgcatt aaagctttat
      601 tatttgctct aactgcagca tcataattat tacctataat ccccatatta ttagcgacgt
      661 atgcaacagt tggtgcagca acatccgaag caattgtaca tataccatct atcttttctg
      721 atatacagat tgctaatatt tcttccttct caataataga aataggataa aatttatcgg
      781 ccacttctgc gcatacggcc ccttctttcc atgcaaaaca aatggtatat atacccattt
      841 ctttagcttt taaaaccaaa ggttcttgta agtagccggc tcctataatt gcaagttttt
      901 tcattaaaat aaattaccat atatctaaaa aactaccttt ctgcacgcat tggattctgt
      961 ataaaatctt gattactata cccctatacc ctcgtaataa aatccgagat tttctaattc
     1021 ttgcttatca taaatattgc gaccatcaat tataattggc gtacgcattg ttttaagcaa
     1081 tacctgccaa cttggcaacc tgaattcttg ccattctgtt accaaacaaa ttgcatcaac
     1141 atttaatgct gcatcatata tatcggaagc aaatgtcagg tttttgtctg tgatgtttat
     1201 ttcctttaca acaggatcga ataccctaag cttacaaccc gcttccaaca acttattaat
     1261 caaaactact gatggggcct cgcgcatgtc atctgtattt ggcttaaatg taagccccca
     1321 tattgcgact gccttatcat tcaaattatt attaaactta ttcgcaagtt tctggtataa
     1381 gatagacttt tgattatcat taacctcttc aacagcattt agcaattgca tattgtagcc
     1441 gttttgctgt gcagttttaa taagagcctt tacatcctta gggaagcaag agccaccgta
     1501 tccacaacct gcatacaaaa acttactacc tatacgagga tcgctaccta tacctcgtct
     1561 tacattgttt atatcagccc ctaccaattc acaaagattt gcaatatcgt tcataaaact
     1621 aatacgagta gccaacatcg aatttgcagc atatttagtc atttcggctg atggtatgtc
     1681 cataaatatt actcggaaat tatttagtag gaatggttta taaagcctgc tcataatttc
     1741 ctttgcagta tcactttcta cgccaacaac cactctgtcg gggctcataa aatctttaat
     1801 tgcagcgccc tcctttaaaa attcggggtt ggaagcaaca tcgaatgtaa gttttgaatt
     1861 acgtttatct aattctgctt gtatagcttg ccttactttt tttgatgtgc caactggtac
     1921 tgtacttttt gtaacaagca atatgtgctt gttcatattt ttacctacag tgcgtgctac
     1981 ttctaataca tatttaagat ctgcactgcc gtcttcatca ggtggagtac ctacagcact
     2041 gaatactact tctactgtat tcagcacctc tgttaaatct gtagtaaaac ttagtcgacc
     2101 ggcagataca tttcttagta ctatttcttc taatcccggt tcaaatatgg gtatttcgcc
     2161 ttgctttaaa gcattgattt tatttgcatc aacatcaaca caagtaacgt ttgtacccat
     2221 ttcggcaaaa caagcaccac ttaccaaacc tacatatcct gtacctacta ttgctatgtt
     2281 catattttat cgtgtaattg tgtaatcgtg taatcgttta atcgtttaat gtttaatgtg
     2341 taatgtgtaa tgtgtaatgt gtaatgtgta atgtgtaatt gtgtaattgt gtaattgtgt
     2401 aatcgtttaa tgtttaatgt ttaatgttta atgtttaatg tgtaatgtgt aatgtttatt
     2461 ttgttgtgct gcgttttatg taactgcgtt tcagtgcaca taatttattt gctattttgt
     2521 ctatataatt ttgttgtaat tcgtctattt gaattttagt aatataactt aaatccattg
//
LOCUS       DAOPID010000211         2567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_56686_length_2567_cov_5.375000, whole genome shotgun sequence.
ACCESSION   DAOPID010000211 DAOPID010000000
VERSION     DAOPID010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56686_length_2567_cov_5.375000"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(370..714)
                     /locus_tag="PK641_06465"
     CDS             complement(370..714)
                     /locus_tag="PK641_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG56022.1"
                     /translation="MKKFTRLVAVSLIALSFFACSSYERIGSFTSISTRNIESKADYV
                     LLTKYTEVKVSAKKAKKQNVDPLTFAAEELVKKQPGGEFIKNAKFFITKDGKKYKIEG
                     DIWGYPMAPIQK"
     gene            complement(773..1021)
                     /locus_tag="PK641_06470"
     CDS             complement(773..1021)
                     /locus_tag="PK641_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HPG56023.1"
                     /translation="MKAVGKELAKSAGVPIYQLDKDQKIGVVLDRFLQRNPGINILLI
                     DDVIYTGKTANLALKNLSRYKQVGKISFYPFAKSQSYK"
     gene            complement(1277..2023)
                     /locus_tag="PK641_06475"
     CDS             complement(1277..2023)
                     /locus_tag="PK641_06475"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HPG56024.1"
                     /translation="MKRNSYFFSDAHLGGRAFSNDKERELKLIRFLDSIKDEASDIYL
                     LGDIFDFWYEYKYVVPKGNVRLLGKMAELADMGIKIHFFIGNHDIWTFGYLQDEIGAK
                     VYTTPQIIDISGKKFYMTHGDMVGYRPLPVRFMQEIFHSRIAQILFSSLHPWFGMKFG
                     LTWSRYNRLNKHLVEPEFMGEDKEYLVQFAKETLKTQAIDYFIFGHRHIMLDLMLANR
                     SRVIYLGDWVHNFSYGVFDGSDFRLEIFEG"
     gene            2200..>2567
                     /locus_tag="PK641_06480"
     CDS             2200..>2567
                     /locus_tag="PK641_06480"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HPG56025.1"
                     /translation="MVESLYEGFLIGLFTSAPVGPIALLCIQRTLQKGRWHGFYSGLG
                     AATSDFLYALIAMLGLSFVMNFIQQNEFLIQVIGSTVVMLFGVYIFFQNPASGLKKNE
                     EGKTSYSQEYVTSFLLTLSNP"
BASE COUNT          780 a          435 c          432 g          920 t
ORIGIN      
        1 acgtttacaa aaaattgctc tttcaggtct gcattgtcga gcaacagtcc aatcaattct
       61 tcgtcaaaat tctgtgcttt gttcagcacc acccattttt tcaattctcc gttgtctgtc
      121 acaaagttgg gctctttctt gagttgttgc tctaatgttt cgtatagttt catctaaaat
      181 tgattttaaa gaatctcata attatgccat tcatagatat ttcaaagaaa attattgaaa
      241 ggaatttttt agtgtttaaa ggtaaaaata atttcacaaa cccaatcaac ttataatcgt
      301 tttatttctc taaaaaaagc acagactaat ttgtctgtgc ttttgtggaa ataaggtaag
      361 gaaaggttat tatttttgta ttggtgccat tggataaccc caaatatcgc cttcaatttt
      421 gtatttttta ccgtcttttg ttataaagaa ttttgcattt ttgatgaact ccccgcctgg
      481 ttgttttttt acgagttctt cagctgcaaa agtcaaagga tctacatttt gtttttttgc
      541 ttttttagcg gatactttta cttctgtgta ttttgtcagt aatacataat cagctttaga
      601 ttcaatatta cgtgttgaaa tagatgtaaa acttcctatt ctttcatagg aactacaggc
      661 aaaaaaagat aaagcaatta aggatactgc tactagacgt gtaaattttt tcatatgatc
      721 tattatttta aaagtttaac actgcaaatg tagtattttt tagttaaaat aatcacttgt
      781 acgattgact ttttgcgaat ggataaaaag aaattttgcc aacttgtttg tatctggaaa
      841 ggtttttaag tgctaaatta gctgtttttc ctgtgtaaat tacatcatcg ataagcagaa
      901 tatttatacc aggattacgt tgcaaaaaac ggtccagtac tacacctatt ttttggtctt
      961 tgtcgagttg gtaaattggt acacctgctg actttgccag ttctttgcct actgctttca
     1021 cgatgttggt tttggaatcg tttgttgcta tagccacaat aatatcaaat ttaatgcctt
     1081 tttttattat ttctttttta aagtcagtta atacgaattt tgtcaggtac tcgagggctg
     1141 ttttttgatt aaaaaacttg gctacaagta ggttattaca ctttcttttt ttcttaaata
     1201 tttaatgatt ttaacttttg ttttgtcaaa tccacaatat caatagaatg ttttgaaaaa
     1261 atcatctatt tctctttcac ccctcaaata tctctaatct gaagtcactt ccatcaaaga
     1321 caccatacga gaagttatga acccagtcgc cgaggtagat tacgcggctg cggtttgcaa
     1381 gcattaagtc gagcattata tggcgatgtc caaaaatgaa atagtcgata gcctgcgttt
     1441 tgagagtttc tttggcaaac tgaacaaggt attctttgtc ctcacccata aattcaggct
     1501 ctacaaggtg tttattaagt ctgttatatc tagaccatgt taaaccgaat ttcattccaa
     1561 accaaggatg tagactgcta aacaaaattt gtgctatgcg cgaatgaaag atttcctgca
     1621 taaaacgaac cggcaaagga cgataaccca ccatatcacc atgagtcatg taaaattttt
     1681 tgccgctaat atctattatt tgtggtgtag tgtaaacctt tgctccgatt tcatcttgta
     1741 agtagccaaa tgtccagatg tcgtgatttc cgatgaagaa atgtatcttt atacccatat
     1801 ctgcgagttc tgccatttta ccgagtagtc tgacattacc cttgggtaca acatatttgt
     1861 actcgtacca aaaatcgaaa atatcaccaa gaagatatat gtctgaagct tcgtctttga
     1921 tgctatctaa gaaacgaatc agcttaagtt ctctttcttt gtcgttggag aatgcgcgac
     1981 cacctaaatg cgcatccgaa aaaaaatatg aattcctttt catgctcaaa tctttgatag
     2041 cgacaaagat aatttaaata cttgaaatat ttatacttgc atcaccatag agttaatgta
     2101 gcgaatatat gttattctaa ttgactaaaa atgcttgcat aaagagaata atttaatcga
     2161 ttgtatgtaa aagccgaaaa aaagtgagat atttgcacta tggttgaaag tttatacgaa
     2221 ggtttcttaa taggcttatt tacatctgca ccggtaggcc caattgcttt gctgtgcatt
     2281 caacgaactt tacaaaaagg tcgctggcac ggtttttatt ccggtttagg tgctgcaaca
     2341 tccgattttc tgtatgcact gatagccatg ttgggattgt cgtttgtgat gaactttata
     2401 cagcaaaacg aatttcttat acaagttatt ggtagtacag tagtaatgct gttcggagta
     2461 tatatctttt ttcagaatcc ggcaagtgga ttaaagaaaa atgaagaagg taaaacaagc
     2521 tactctcaag agtatgttac ctctttttta ttgacactaa gtaatcc
//
LOCUS       DAOPID010000212         2560 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_56889_length_2560_cov_5.404391, whole genome shotgun sequence.
ACCESSION   DAOPID010000212 DAOPID010000000
VERSION     DAOPID010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56889_length_2560_cov_5.404391"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1466
                     /locus_tag="PK641_06485"
     CDS             <1..1466
                     /locus_tag="PK641_06485"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPG56026.1"
                     /translation="NIIFDNQIVPKEYTNHSNRKEFAIAKKQGQGFAIRKSDTTKQSY
                     FYYIKKYNKHYIRTAKPFNRLSDVLETDNLAIILILFFFLSASGAFYMVARRFGNTIN
                     ALKNLIEKVDSGDRYNFPDNEFREISEYIVNMYKRLDHTQKALNMEREKLMSHLKLSK
                     RGLAIFSPQRKEILSNELFIQYINLISDKQRRYPESVFDISQFGDITEFLDDRQRNDT
                     NIFETKSIQIHKNDSIISIHCIIFPDKSFEITIDDITQQEEQALLKRQLTQNISHELK
                     TPISSIQGFMETLLNNPDMEEDKKTFYIERCYNQAIRLSYLLQDISMLNKLDEASNIF
                     AKDDLDLHNIIDSVVQDVALELEEKNFNVHVDVASPLPIKGNQSLLYSVFRNLLDNSL
                     HYAGSDINIEISCYRSDKEFYYLSFADNGVGVEETHLNRIFDRFYRVDKGRSRKLGGT
                     GLGLAIVKNAIHFHQGRISAKNRQGGGLEFLFTLRKE"
     gene            complement(1469..>2560)
                     /gene="pepD"
                     /locus_tag="PK641_06490"
     CDS             complement(1469..>2560)
                     /gene="pepD"
                     /locus_tag="PK641_06490"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HPG56027.1"
                     /translation="AVASMMAVLASTNIKHPALECLFTVDEEIGMLGAFGLDSNALKG
                     EVLLNLDSEEEGELIVGCCGATRAECKFMYSKVSVPKGDAAYKISIQNLKGGHSGLDI
                     HLQRANAIKCLSHFLKTLVRDFEARVASIEGGTVCNAIPNEASAIITIPAEGEDDLYG
                     VAQEFQEIFENEYKLSDPEFELTVESVSLPEGLLPESVQDDIINAIVAVPNGVYRYSP
                     TLKDVVETSSNLGSIHTKDNNIVLDFLFRSTLDSMKGEVKSMIESIFLLAGASVRFWG
                     NYDGWELDMESEILKKAVKCYKYLYNVEPRVSIIHAGLECGIFSSIYPNLDMLSFGPT
                     IKYPHSVNENLDINSVERFWKLLVAILED"
BASE COUNT          911 a          469 c          383 g          797 t
ORIGIN      
        1 gtaatattat tttcgacaat cagatagtgc ctaaggaata cacaaatcat tctaatagaa
       61 aggaatttgc aatagcaaaa aaacaagggc aaggatttgc aattcgaaaa tcagacacta
      121 caaaacaatc atatttttat tatataaaga aatataacaa gcattatatt cgaacagcta
      181 aaccttttaa taggctatct gatgtactcg aaacagataa tcttgcaata atactaatat
      241 tgtttttctt cttatctgca tcaggcgcat tttatatggt tgcaagacgc ttcgggaata
      301 ctataaatgc tttaaaaaat cttattgaaa aggtagattc aggtgataga tataatttcc
      361 cggataatga attcagggaa attagcgaat acatagttaa tatgtataag agacttgatc
      421 atactcaaaa ggctttaaat atggaacgag aaaaactaat gtcacacctt aagttatcca
      481 aacgaggctt ggctattttt tctccgcaac gcaaagaaat tttatcgaac gaattattta
      541 ttcaatatat aaacctcatc tcggataaac aaagacgata tcctgaaagt gttttcgaca
      601 tatcccaatt tggagatatc acagagtttt tagatgacag acaacgtaac gacacaaata
      661 tatttgaaac aaagtcaata caaatacata aaaatgactc tataatatca atacattgta
      721 tcatttttcc tgacaagagt tttgaaataa caatagatga tattacacag caagaagaac
      781 aagctcttct gaaacgtcaa cttacacaaa acatatcaca cgagttaaaa actcctataa
      841 gcagcataca gggctttatg gaaacgcttc tcaataatcc tgatatggaa gaagataaaa
      901 aaacgttcta tatagaaagg tgttataatc aggctattag attaagttat ctattacaag
      961 acatatcgat gcttaacaaa cttgatgaag catctaatat ttttgcaaaa gacgatcttg
     1021 atttacacaa cattattgat agcgtagtcc aagatgttgc cctagaattg gaagagaaga
     1081 actttaatgt tcatgttgac gtagcatcgc cactccctat taaaggcaat caatctttac
     1141 tatattcggt atttaggaat ctattagaca atagtcttca ctatgcaggt tctgatatta
     1201 atattgaaat aagttgctac cgaagcgata aagaatttta ttatttatct tttgcagaca
     1261 atggcgttgg tgttgaagaa acacacctaa atcgtatttt tgatagattc tatagagttg
     1321 acaaaggaag atcaaggaaa cttggaggaa caggtttagg tttagctatt gtaaaaaatg
     1381 caattcattt ccatcaaggt cgtatatcag caaagaatcg tcagggtggc ggacttgaat
     1441 tcctatttac tctccgtaaa gagtagagtt aatcctccaa tattgcaact aacaatttcc
     1501 aaaaacgttc tacactatta atatctaagt tttcattaac tgaatgcgga tacttgattg
     1561 ttggtccgaa tgacagcata tccaaattcg gatatataga gctaaaaata ccacattcca
     1621 aacctgcatg aataatactt acacgaggct ctacattata taaatactta tagcatttta
     1681 cagctttttt gagtatttca gattccatat ctagttccca accatcataa tttccccaga
     1741 aacggactga tgcacctgct aataaaaata tgctctcaat catcgatttt acttcacctt
     1801 tcatcgaatc taaagtgctt ctaaacaaaa aatcaagtac aatattatta tcttttgtat
     1861 gaatactgcc taaattagag gatgtttcta ctacatcttt taatgtcgga gaatatctat
     1921 atacaccatt tggtacggca actatagcat ttataatatc atcctgtacc gactcaggta
     1981 ataaaccttc cggtaatgat actgattcaa ctgtcaattc aaattcaggg tcagataatt
     2041 tatattcatt ttcaaatatc tcctgaaatt cttgagcaac tccatataaa tcatcttcac
     2101 cttctgccgg aattgtaata attgcagatg cttcattagg tattgcatta cataccgtac
     2161 ctccttcaat agatgcaact ctggcttcaa aatctctaac taatgttttt agaaaatgtg
     2221 acagacattt aatcgcatta gcacgctgta agtgtatatc taaacccgaa tgcccacctt
     2281 taagattttg tatcgaaatt ttatatgccg catcaccttt tggaacagac actttagaat
     2341 acataaattt acactctgcg cgagttgcac cacaacatcc cactatcaat tcaccttctt
     2401 cttccgaatc aagattaagc aaaacttctc ccttcaatgc gtttgaatca agtccaaagg
     2461 ctccaagcat accaatttct tcatctaccg taaatagaca ttctaaagcc gggtgtttaa
     2521 tgttagttga tgctaaaaca gccatcattg aggctacggc
//
LOCUS       DAOPID010000213         2552 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_57135_length_2552_cov_4.401282, whole genome shotgun sequence.
ACCESSION   DAOPID010000213 DAOPID010000000
VERSION     DAOPID010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57135_length_2552_cov_4.401282"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..203)
                     /locus_tag="PK641_06495"
     tRNA            complement(130..203)
                     /locus_tag="PK641_06495"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(167..169),aa:Pro,seq:tgg)
     gene            complement(250..1236)
                     /locus_tag="PK641_06500"
     CDS             complement(250..1236)
                     /locus_tag="PK641_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HPG56028.1"
                     /translation="MPVTLYPMKFRPILKQVLWGGSKICSYKNLDVQESKVGESWEIS
                     DVKGSVSVVAEGALAGMNIDDLLEKYQELFVGEKNYQRFGNEFPLLVKFIDAEQDLSV
                     QVHPNDEMARKKHQSNGKTEWWYILDSKPDAYLYAGFNRNIDKQIFSKSLNDNTVCEL
                     LNKEMVQPGDVFFLPAGRIHSICSGIFLAEVQQTSNVTYRVWDYNRKDANGNLRELHT
                     DLALDSLDYHHYKQYKTSYKTRLNKQVKLQECSYFTVNLVEADTVMSFDYKGLDSFII
                     WMCVEGSAVYASSKKHKGHITRGETILFPACDQPVAVIPEIDRVKFLEIYVE"
     gene            complement(1249..2517)
                     /gene="serS"
                     /locus_tag="PK641_06505"
     CDS             complement(1249..2517)
                     /gene="serS"
                     /locus_tag="PK641_06505"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPG56029.1"
                     /translation="MLTLKYITDNTDEVIARLAKKHFDGKESIGKVVELDSKRKKTQA
                     QLDACLSEINQLSRQVGDLYKQGKTEEANALKTKTAELKEASKDFSASMDTIETEITD
                     ILLTVPNIPNPNVPEGVGAADNLVERIGGVIPELPEDALPHWDLAKKYDLIDFELGVK
                     ISGAGFPVYKGQGARLQRALINFFLDEAREAGYTEIMPPTVVNAASGYGTGQLPDKEG
                     QMYNCNLDDLYLIPTAEVPVTNIYRDVILTEQYLPIKNCAYTQCFRREAGSYGKDVRG
                     LNRLHEFSKVEIVRIDTPEHSYQSLEEMIAHVENLVKKLELPYRILRLCGGDMSFTSA
                     ITFDFEVFSSAQKRWLEVSSVSNFESYQANRLKCRYRVDKKTALCHTLNGSALALPRI
                     VAALLENNQTPEGIRIPKSLVPYTGFDMIK"
BASE COUNT          807 a          547 c          394 g          804 t
ORIGIN      
        1 cctaacgaac tgctaaacac ggctcaaaaa tactggtcaa aacctatgtg cgaagtatta
       61 gtaggtgatt ttaaagcaga taaataaaca aaataaaaaa gactaacctt aaaagttagt
      121 ctttttttgt cgggatgaca ggattcgaac ctgcgaccac ctggtcccaa accaggcgcg
      181 ctaccggact gcgctacatc ccgcaaaagc agcacaaagg tataactatt ttttcaataa
      241 acaagcaaat tattccacat aaatttccaa aaatttcacc ctatctattt caggtatcac
      301 tgctacaggt tggtcacaag ccgggaataa tatggtttct cctcttgtaa tatgtccctt
      361 gtgtttcttt gaagaagcat atactgcact tccttcaaca cacatccata taataaaaga
      421 gtctaaaccc ttgtaatcaa aagacataac ggtatcggcc tcaacaagat taactgtaaa
      481 gtaagaacat tcttgcagtt taacttgttt atttagtcga gttttatatg aagttttgta
      541 ttgtttataa tgatgataat ctagcgaatc caatgccaaa tcagtatgta attcacgtaa
      601 attaccatta gcatcctttc tattataatc ccatacacga taagttacat ttgaagtttg
      661 ttgcacttca gccaagaaaa ttccgctgca tattgaatga attcgacctg ccggcaaaaa
      721 aaacacatcg ccgggttgca ccatctcctt attcaataat tcacatacag tattatcgtt
      781 taaacttttt gaaaatattt gtttatcaat attccgatta aatcctgcat acaaataagc
      841 atcgggctta gagtctagta tataccacca ctctgtcttt ccattagatt gatgcttctt
      901 acgagccatc tcatcattcg gatgaacttg cacagataag tcctgctcag catcaataaa
      961 tttaaccaac aggggaaatt catttccaaa acgttgataa tttttttctc caacaaaaag
     1021 ttcctgatat ttttctaata aatcatcaat attcataccg gccaatgctc cttctgcaac
     1081 aacagaaaca ctacccttta catcagagat ttcccaactt tcaccaacct tggattcttg
     1141 cacatccaga ttcttataag aacatatttt cgagccaccc cacaacacct gtttgaggat
     1201 gggtctaaat ttcattggat atagggttac cggcataata atgctttttt atttaatcat
     1261 atcaaatccg gtatatggca caagagactt cggaatacga attccttcag gtgtttgatt
     1321 attttccagc aatgcagcaa ctatacgcgg caatgccaat gcgctaccat ttaatgtgtg
     1381 acataatgcc gtttttttat ctacacgata acgacatttc aaacggtttg cttggtagct
     1441 ttcgaaattt gatacagagc taacttccaa ccaacgcttt tgcgcagatg agaatacttc
     1501 aaaatcgaat gttattgcag aagtaaatga catatcacca ccacaaagac gtaatatacg
     1561 atatggcaac tccaattttt taactaagtt ctcgacatgt gcaatcatct cttctaaact
     1621 ctgataagaa tgttcaggag tatcaatacg aactatttct actttagaaa attcgtgaag
     1681 acgatttaat ccacgtacat ctttaccata agaaccggcc tcacgacgaa aacattgtgt
     1741 gtatgcacaa ttcttgattg gcaaatattg ttctgtaaga ataacatcac gatagatatt
     1801 ggtaacaggt acttctgccg tcggaatcaa atacaaatca tctaaattgc aattatacat
     1861 ttgaccttct ttatcaggca attgacccgt tccgtaaccg gatgccgcat ttacaaccgt
     1921 agggggcatt atttctgtat atcctgcttc acgagcctcg tctaaaaaga aattgatcaa
     1981 agcacgttga agtcttgcac cctgtccttt ataaacaggg aagccggctc ccgaaatctt
     2041 aacgcctaat tcaaaatcta taagatcata tttctttgcc aaatcccaat gaggaagtgc
     2101 atcttcaggc aattcgggga taacaccgcc aatgcgttct acaagattat ccgcagcacc
     2161 tacgccttcc gggacattcg gatttggtat atttggaact gtcaacaaaa tatctgtaat
     2221 ttcagtttca attgtatcca tagatgcaga aaaatccttt gaagcctctt ttaattctgc
     2281 agttttagtt tttaaagcat tggcttcttc tgttttacct tgtttgtaca aatcacctac
     2341 ttgacgtgat aattggttaa tttctgacaa acatgcatcc aattgtgctt gtgttttttt
     2401 acgtttgcta tctaactcaa ccacctttcc tattgattct ttgccatcga aatgcttctt
     2461 tgccaaacgg gcaattacct catcagtatt atcagtaatg tacttaagtg tcaacatata
     2521 tttaaatttt acaattcgac ctcaaaggta ta
//
LOCUS       DAOPID010000214         2497 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_58870_length_2497_cov_3.958231, whole genome shotgun sequence.
ACCESSION   DAOPID010000214 DAOPID010000000
VERSION     DAOPID010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58870_length_2497_cov_3.958231"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /gene="argS"
                     /locus_tag="PK641_06510"
     CDS             complement(<1..898)
                     /gene="argS"
                     /locus_tag="PK641_06510"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008023236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPG56030.1"
                     /translation="MEQELIANIQSVVKQLYGQEVDASILSLQKTKKEFEGDLTLVVF
                     PLLKISHKKPEETAQEIGNKLKSDCPLVSDFNVIKGFLNLTLADKGFVDTLASIDSDE
                     YFGFKKATETSPLIMVEYSSPNTNKPLHLGHIRNNLLGYSLSKILEANGNKVVKTNIV
                     NDRGIHICKSMLAWQKFGAGATPESTSLKGDHLVGDYYVAFDKAYKAEVKQLMEEKSM
                     LEDDAKRQAPLMLEAQEMLRKWEANDADVRNLWKTMNEWVYKGFDETYKRLGVSFDKI
                     YYESETYLVGKDKVAEGLSKGIF"
     gene            complement(982..1254)
                     /locus_tag="PK641_06515"
     CDS             complement(982..1254)
                     /locus_tag="PK641_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPG56031.1"
                     /translation="MNKTELISAIAANSGLSKVDSKKALDGFMKAVSQSLKKGDKVSL
                     VGFGSFAVAKRSARVGVNPATKKKMTIAAKKVVKFKAGAELADKVK"
     gene            1690..2454
                     /locus_tag="PK641_06520"
     CDS             1690..2454
                     /locus_tag="PK641_06520"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPG56032.1"
                     /translation="MDSYKPINPNFPPAIKNLIIINFIFWLASIILPKYANIDIVDWL
                     GLHYWLSDKFNPAQLITYMFLHDTSSFSHIFFNMFGLWMFGKNIEMFWGTKRFLIFYF
                     VTGIGAGIIQELTWMVDYVPIVNAINEYASSKDISQLMPYLKNIQENTIIPIDQLMDF
                     KTQILNQPITVGASGALFGILLAFAMLFPQARMFMIFIPIPIRAPYFVAFYALAELFM
                     GIGGNADGIAHYAHLGGMLFGLILILIWKKKGTLYQ"
BASE COUNT          813 a          452 c          349 g          883 t
ORIGIN      
        1 caaatatgcc ttttgacaaa ccttctgcta ctttatcttt accaactaaa tatgtttctg
       61 attcgtaata tattttgtcg aatgatacgc ctaatctttt ataagtttcg tcaaaaccct
      121 tataaaccca ttcattcatc gttttccata aattgcgtac atctgcatca tttgcttccc
      181 acttacgcaa catctcttgt gcctcaagca tcaatggagc ttgtctcttt gcatcatctt
      241 ccaacatcga cttttcttcc atcaattgct taacttcggc cttgtaggcc ttatcaaaag
      301 caacgtagta atctcctacc aaatgatctc cttttaaaga agtagattcg ggagttgcac
      361 ctgcaccaaa tttttgccat gccaacattg acttgcaaat atgtataccc ctatcattaa
      421 ctatatttgt ttttaccact ttgtttccgt tagcttctaa aattttagac aagctataac
      481 ctaacaagtt atttcggata tgtcctaagt gtaatggttt atttgtattg ggagatgaat
      541 attcgaccat aatcaatggc gaagtttctg tggctttctt aaaaccgaaa tattcgtctg
      601 aatcaataga tgccaatgta tcaacaaaac ctttatctgc caatgtcagg ttcaagaacc
      661 cttttattac attaaaatcc gaaaccaaag gacaatctga ttttagttta tttcctattt
      721 cttgagctgt ttcctccggt tttttatgag atatctttaa caaagggaac acgactaagg
      781 tcaaatcgcc ttcaaattct ttttttgttt tttgcaaaga caaaattgaa gcatccacct
      841 cttgtccgta aagttgtttt acaacgctct gaatatttgc tattaattcc tgttccatat
      901 tatgtgatac aaattatagg acacaaaagt acaaaaaaaa gagcaccttt cgatgctctt
      961 ttttaattta tgcttattga cttatttaac tttatcagcc aactctgcac ctgctttaaa
     1021 tttaacaacc ttcttggctg caatagtcat ttttttctta gttgcaggat taacaccaac
     1081 acgtgcgcta cgtttagcta ccgcgaaaga accaaaccca actaaagaca ctttatcacc
     1141 ttttttcaaa gattgagaaa ctgctttcat aaaaccatcc aaagcttttt tagaatcaac
     1201 tttgcttaag ccagaattag ctgcaattgc gctaattaat tcagttttgt tcatactttt
     1261 tacaatttta ggttaacaaa tagatttatt tatgtgtgat tttttgtaag gtctcgtaca
     1321 actacctgca aatataatgc ttataaattc atgcgcaact cctatactaa aaaaatacat
     1381 ttaaaaatat aagaaaacta aataattaac tttattttag tataaatttt aactattgat
     1441 taaatgggtt ctctatcgta taggatatat tacttaatgc ttgacataat gaattaaaat
     1501 tatattaatg ccacaaacaa ataacatatt atatcatttg taaaaaaaat gatacaaaaa
     1561 aaatcaaaca cactacttat gtatatgtac acttatacag atattataag tatacaaaaa
     1621 aacattaatt agaagggtac ttcactaaat tttcatactt ttgtaattgc aaaaaacaca
     1681 gagcatatca tggactcata caaacctata aaccctaatt ttccacccgc tatcaagaac
     1741 cttataataa ttaattttat tttctggcta gcgtcgatta ttcttccaaa atatgcaaat
     1801 atagatattg tggattggtt gggattgcat tattggttgt cggacaaatt caatccggct
     1861 cagttaatca catatatgtt tttacacgac acaagcagtt tttcacatat atttttcaat
     1921 atgtttggtt tgtggatgtt tggaaaaaac attgaaatgt tttggggtac aaaacgattt
     1981 ctcatattct attttgtaac cggtatagga gctggtataa ttcaagaatt aacttggatg
     2041 gtagattatg ttcccattgt aaatgcaata aatgagtatg catcaagcaa ggatatttcg
     2101 caactgatgc cgtatctaaa aaatatacaa gaaaatacta ttattccgat agatcaacta
     2161 atggatttca agactcaaat acttaatcaa cctattacag ttggtgcatc cggtgcttta
     2221 ttcggtattt tattggcatt tgcaatgcta ttcccacagg ctagaatgtt tatgatcttt
     2281 ataccaattc ctatacgtgc tccttatttt gttgcatttt acgcattagc agaattgttc
     2341 atgggaattg gaggtaatgc tgacggcata gcacactatg cacacttagg cggcatgctt
     2401 ttcggtctta tattaatatt aatatggaag aagaaaggta ctttgtacca ataaatcatg
     2461 tctattgcat ctgaattaaa aattagcttt aaagaag
//
LOCUS       DAOPID010000215         2494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_58972_length_2494_cov_3.651907, whole genome shotgun sequence.
ACCESSION   DAOPID010000215 DAOPID010000000
VERSION     DAOPID010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58972_length_2494_cov_3.651907"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="PK641_06525"
     CDS             <1..634
                     /locus_tag="PK641_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HPG56033.1"
                     /translation="WYPGDKDGKGGIYNFVTKRALCKGVDSKVFWTQVETGSAITWKY
                     PSCILRGDNSVGEFYSVAVTNNYQQADTGTKMIHIGKNTRSRIVSKGISAGHSQNSYR
                     GLVKMSENADFARNFSQCDSLLLGDKCGAHTFPSIDVHNQTAIVEHEASTSKINEDQL
                     FYCNQRGISTEDAVGLIVNGFAKEVFNKLPMEFAVEAQKLLQISLEGTVG"
     gene            636..1388
                     /gene="sufC"
                     /locus_tag="PK641_06530"
     CDS             636..1388
                     /gene="sufC"
                     /locus_tag="PK641_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HPG56034.1"
                     /translation="MLEIKDLHAVIGGKEILRGVNLSVKPGEVHAIMGPNGSGKSTLS
                     AVLAGHPAYSVTGGTVTFNGKNLLELSPEERSREGLFLSFQYPVEIPGVSMVNFMRTA
                     VNEHRKYKNLPQLSAAEFLKLMRDKKNIVELDSKLANRSVNEGFSGGEKKRNEIFQMA
                     MLEPSLSILDETDSGLDIDALRIVANGVNKLKRPDSATILITHYQRLLDYIVPDVVHI
                     LYKGRIVKTAGKELALTLEEKGYDWIKQEVGE"
     gene            1390..>2494
                     /locus_tag="PK641_06535"
     CDS             1390..>2494
                     /locus_tag="PK641_06535"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HPG56035.1"
                     /translation="MVQDAYISLYKEQSELIDSYSSDLLNTNRAAACESLSKLGLSIY
                     AQEYERDFGLNINRYKINVDPYLSFKCDVPNIGAYIYFVLNDTFYPNSQQAKLPEGVI
                     VCGISEAISKYSDLVKPYINRQMTINTDGNQAFNAMFAQDGLFVYIPKGLKMDKPLQL
                     INLMHGNSDILAVSHNLIILEDDSSAQLLICDHASGDSHYLTNRVTEVFVGKNAIYEH
                     YKLENTHEKMTNICSLLLDQADNSQVLSNIITLHNNKTRNTVVANINGSHSSLSLNGL
                     VLGDKDQNIDNTTTINHIGPDSSSAELFKYVLDGQAKGKFYGMVKVYPNAQKTSAIQT
                     NRNMCISNKANMQTKPQLEIYADDVKCNHGATVG"
BASE COUNT          893 a          390 c          474 g          737 t
ORIGIN      
        1 ctggtatcca ggcgacaaag acggcaaagg tggcatatac aattttgtga caaaaagagc
       61 attatgtaag ggcgttgatt caaaagtttt ttggacacaa gttgaaacag gctctgcaat
      121 tacatggaaa tatccaagtt gtatattacg cggagataat tcggtaggcg aattctactc
      181 agttgctgta acaaataact atcaacaggc tgatacaggg actaaaatga tacatatcgg
      241 caaaaatact cgtagtagaa ttgtatcaaa aggtatttct gccggacata gtcaaaactc
      301 atacagagga ttggtaaaaa tgtctgaaaa tgctgatttt gcacgtaatt tttcacaatg
      361 tgacagcctt ttgttaggcg ataagtgcgg agctcataca ttcccttcaa ttgatgttca
      421 taaccaaaca gcaatagttg aacatgaagc gagtacctca aaaatcaatg aagatcaact
      481 tttctattgt aatcaacgcg gtatttcaac agaagatgct gtaggtctta ttgtaaatgg
      541 tttcgctaaa gaagtattta ataaattacc aatggaattt gcagtagaag cacaaaaact
      601 acttcaaata tctctagaag gaacagtagg ataatatgtt ggaaataaaa gatttacatg
      661 ccgtaattgg cgggaaagag atacttagag gtgttaacct ttcggttaaa ccgggtgaag
      721 ttcatgccat catgggacct aatggttccg ggaaaagtac actatcagct gttcttgcag
      781 gtcatcctgc ttattcagta accggtggaa cagtaacttt caatggtaaa aacttattag
      841 aattatcgcc ggaggaacgt tctcgcgaag gattattctt gagttttcaa tatccggttg
      901 aaatccccgg agtaagcatg gtaaatttta tgcgtacagc agtcaacgag catagaaaat
      961 ataaaaatct gccacaatta tcagctgcag aattccttaa gttgatgcgt gacaaaaaaa
     1021 atattgtaga attagacagc aaactcgcta atcgctcagt taatgaaggt ttttcgggag
     1081 gagaaaaaaa acgtaacgaa atatttcaaa tggcaatgtt agagccatct ttgtctattt
     1141 tagatgaaac agattccgga ttagacattg atgctttaag aattgttgca aatggcgtaa
     1201 ataaattaaa acgtcctgat tcggctacca ttttaataac ccactaccaa aggctactag
     1261 actacatcgt gcctgatgtt gtacacattt tatataaagg cagaattgtt aaaacagcag
     1321 gtaaagaatt ggctttaaca ttggaagaaa aaggatacga ttggattaaa caagaagtag
     1381 gagaataaga tggttcagga tgcctacata tcattatata aagaacaatc agagcttata
     1441 gattcttata gttcagatct attaaatact aatagagcag ctgcttgtga atccttgtca
     1501 aaattaggtt taagcattta tgcacaagaa tatgagcgag attttggttt aaatattaat
     1561 agatataaaa ttaatgttga tccttatttg tcatttaaat gtgatgtccc gaatattgga
     1621 gcttatatat attttgtttt aaatgacact ttttatccca attcgcaaca agcaaaatta
     1681 ccagaaggag taatagtttg cggcatatca gaagcgatta gtaaatattc tgatttggtt
     1741 aagccctaca ttaatcgtca aatgactatt aatactgacg ggaatcaagc tttcaatgcg
     1801 atgtttgctc aagatggcct attcgtttat attcccaaag ggcttaaaat ggataaacca
     1861 ttgcaattaa taaatctgat gcatggcaat tcagacatat tagctgtaag tcataattta
     1921 ataatattgg aagatgattc atctgctcag ttactaatat gcgatcatgc atcaggagat
     1981 tctcattatt tgacaaatag agtaactgaa gtatttgttg gtaaaaatgc aatatacgaa
     2041 cattacaaat tagagaacac tcacgaaaaa atgactaata tttgttcttt gttgttagat
     2101 caagctgata acagtcaggt gctatcaaat attattactc tacataataa caaaactaga
     2161 aacacagtag ttgcaaatat taacggatct cattcatcat tatctttaaa tggtttggtc
     2221 ttgggtgata aagatcaaaa tattgataac acaactacga ttaatcatat cggaccggat
     2281 tcttcaagtg ctgaattgtt taaatatgtg ttagatggac aagctaaagg caaattctat
     2341 ggtatggtaa aagtctatcc aaatgctcaa aaaacatctg caattcagac aaaccgaaat
     2401 atgtgcattt caaataaagc aaatatgcaa acaaaaccgc agctcgaaat atatgcagac
     2461 gatgttaagt gtaatcacgg agcaacagtt gggc
//
LOCUS       DAOPID010000216         2446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_60573_length_2446_cov_4.249268, whole genome shotgun sequence.
ACCESSION   DAOPID010000216 DAOPID010000000
VERSION     DAOPID010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60573_length_2446_cov_4.249268"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="PK641_06540"
     CDS             <1..1074
                     /locus_tag="PK641_06540"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPG56036.1"
                     /translation="VHKWPKCTSSNYRMTAWTDPHGADIDRQVLEKGWFSYNMPDLNL
                     ENPLLFKYLSQVYIWWIEYGNVDGIRVDTYPYNNINIASAFMKIFRDEYPAINIVGEC
                     WVKSPLEISYYQSGNNNKDGFDSNLPSVMDFVLKDVLHMAFNENDGWDSGMSRFYSHY
                     AQDFAYPNINNLMNFLDNHDIDRFSGSVDYDINKYKMGVAMLLTTRGFPQIYAGDEIM
                     LDGIQGNYEGYRFDFPGGWKEDSRNAFTQEGRTPKENEVFNYMRKILNYRKQNTALQT
                     GKMKQFIPYDGIYLFSRYDQSKTIVMIFNNNNQTKVVSLERYEEVFDNYTSGTEITTE
                     KQIDLTQYIAIPAKTAWIIELNK"
     gene            1117..2424
                     /locus_tag="PK641_06545"
     CDS             1117..2424
                     /locus_tag="PK641_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007214797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPG56037.1"
                     /translation="MNRPDLSFAKLFNISFGFFGVQIAYALQSANISRIFSTLGADPH
                     NLSYFWILPPLMGIIVQPIVGWASDKTWIKSIGRRKPYLLLGSFVAILVMCLLPNAGS
                     YQKYIDAMTFGLIMLMLLDTSINMAMQPFKMMVGDMVNEKQKGLAYSIQSFLCNAGSL
                     IGYLFPFIFTWIGISNTAAEGIIPDSVIYSFYIGAAILILCVLYTSFSVKEMNPEEFN
                     AYHNITESENKKSENVLSLLIHAPKQFWTIGLVQLFSWVAFMYMWTYTHGAIAENVWG
                     TTETTSEGFQAAGNWVGVLFAVQAIGSVCWAILLPLFKSRKLGYSLSLLLGGIGFIST
                     LFIHNQYILFASYFIIGFAWAAMLAMPFAIFTNSVSGKNMGIYLGLFNGTICLPQIIA
                     AIIGGDILKMVGGAQVSMLAVAGASLILGACSVFIISEKKTNS"
BASE COUNT          824 a          400 c          456 g          766 t
ORIGIN      
        1 gtccacaaat ggccgaaatg cacttcatca aattatcgca tgacagcatg gacagatcca
       61 catggagcag atattgacag acaagttttg gagaaaggct ggttctcgta caatatgcct
      121 gacttaaatt tagagaatcc attattattc aagtacttaa gtcaagtata tatttggtgg
      181 attgaatatg gaaatgttga tggaataaga gttgatacct atccttataa caacatcaat
      241 attgcatctg cgtttatgaa aatctttaga gatgaatatc ctgcaattaa catagtagga
      301 gaatgttggg ttaaatcacc acttgaaatt tcctattacc aatctggaaa caacaataaa
      361 gatggatttg attcaaatct tccttctgtt atggattttg tgttgaaaga tgtgctacac
      421 atggcattta atgaaaatga tggctgggat agcggaatgt ctcgttttta ctcacattat
      481 gcacaagact ttgcataccc caacatcaac aacctaatga attttcttga caatcacgat
      541 attgaccgct ttagtggatc tgttgattat gacataaaca aatacaaaat gggagttgca
      601 atgttgctaa caacacgcgg atttccacaa atatatgccg gagatgaaat catgttagat
      661 ggtattcagg gaaattacga aggataccgc ttcgatttcc ccggtggatg gaaagaagat
      721 tcacgcaatg catttacgca agaaggacgc actcctaaag agaacgaagt gtttaactac
      781 atgcgaaaaa tacttaatta taggaaacaa aatactgccc tacagacagg aaaaatgaaa
      841 caatttattc cttatgacgg catatattta ttttcgagat acgaccaaag taaaacgata
      901 gtaatgatat ttaataacaa taatcaaact aaagttgtat ctcttgaacg ctatgaagaa
      961 gtttttgata actatacaag cggaacagaa attactacag aaaaacaaat tgacttgaca
     1021 cagtatatag ctatacctgc aaaaactgca tggattattg aactaaacaa gtaaaaacta
     1081 gaaatataat acagcctaac ttaactatta aacgatatga acagaccgga tttgtccttt
     1141 gcgaaactat ttaatattag tttcggcttt ttcggagtgc aaattgctta tgctttacaa
     1201 agtgctaaca taagcagaat tttctctaca cttggagctg atccacataa cttaagctat
     1261 ttttggatat tacctccact aatgggtatt atagttcaac ctattgttgg ttgggctagc
     1321 gacaaaacat ggattaaaag cattggcaga cgtaaaccat acttactttt aggttcattt
     1381 gttgctattt tagtaatgtg ccttcttcca aatgccggca gttaccaaaa atatattgat
     1441 gcaatgacat tcggcttaat tatgcttatg ctgcttgata catcaattaa catggcaatg
     1501 caaccattta aaatgatggt tggagatatg gtaaacgaga agcaaaaagg attggcctac
     1561 tctattcaga gttttttatg taatgcaggt agtttaatag gatatttatt cccattcata
     1621 tttacatgga ttggcataag caataccgcc gctgaaggta taataccgga ttctgtaata
     1681 tactcattct atataggcgc tgcaatttta atattatgcg ttttatacac aagtttttct
     1741 gttaaagaaa tgaatccgga ggagtttaac gcataccata acatcaccga atctgaaaac
     1801 aaaaagtctg agaacgtatt atctttatta atacatgccc caaaacaatt ttggacaatc
     1861 ggacttgttc aattattctc gtgggtagca tttatgtata tgtggacata cactcacgga
     1921 gcaattgccg agaacgtatg gggtacaaca gaaactacta gtgaaggatt tcaagctgca
     1981 ggtaattggg ttggcgtttt atttgcagta caagcaatcg gttctgtttg ctgggcaata
     2041 ttacttcctc tgtttaaatc tagaaaactc ggatactcgt tgagtttgtt acttggtggc
     2101 attggattta taagtactct gtttattcac aaccaataca tactttttgc atcatatttt
     2161 ataataggtt ttgcatgggc tgcaatgctt gcaatgccat ttgcaatttt cacaaactct
     2221 gtatcgggca aaaacatggg aatttaccta ggtttattta atggaactat ttgtttaccg
     2281 caaataatag ctgcaattat tggtggagat atattaaaaa tggtaggagg agctcaagta
     2341 agtatgcttg ctgttgcagg ggcttcatta attcttggag cttgttcggt atttatcatt
     2401 agcgagaaga aaacaaatag ttaaataagt aataagttta taaaac
//
LOCUS       DAOPID010000217         2445 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_60605_length_2445_cov_5.516318, whole genome shotgun sequence.
ACCESSION   DAOPID010000217 DAOPID010000000
VERSION     DAOPID010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60605_length_2445_cov_5.516318"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PK641_06550"
     CDS             complement(<1..408)
                     /locus_tag="PK641_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HPG56038.1"
                     /translation="MDNTKIRVRFAPSPTGALHIGGVRTALYNYLFAKKHAGSMILRI
                     EDTDSQRFVPGAEKYIIEALSWLGIKFDEGIENGQATCRQSERKEIYSKYVDQLLDAG
                     KAYIAFDTPSELDAKRQEIPNFQYDAHTRGLMKN"
     gene            517..1674
                     /locus_tag="PK641_06555"
     CDS             517..1674
                     /locus_tag="PK641_06555"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPG56039.1"
                     /translation="MKSSKQKIILIAQILLIVSTQLMISQTKAVVVDKTTKQPIPYVS
                     IYTKNADLVLGSMSNEQGQFEIDFSFNTLFLSHINYGKVEIKKENVQDTIFLIPTSVL
                     LNEVVVNKRQPKWINAVLSRVIKGKGKNYQTLEKTFSYKYETYTLTDSNGYEFKSKGN
                     IEFPKMKKDVQFKIDAQSNTIKYKNKTAGVDFSNLRRMLYDDFVNGFDNKFLNEYDFS
                     QNSSFVGEDKNVIQLIFSSKKFQDDEGYIVLDTLNNVILEAEKNSGTECNIKTKTSSI
                     LRNVVASRGFNYDTWITKSNTKYSKIGDCYYISECKYKFYMKSVYKNKKVDSEYFTSI
                     ESKLFLSDKIERASTSFKILPKPYYIVAIMTKKMRLEEEALNKVPVSFEGF"
     gene            1885..>2445
                     /locus_tag="PK641_06560"
     CDS             1885..>2445
                     /locus_tag="PK641_06560"
                     /inference="COORDINATES: protein motif:HMM:NF023583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HPG56040.1"
                     /translation="MPLNILQYESDIWSSADSLRSAGLKQSDFPKYMMPFFALRMVES
                     RLIREHKRLSAEFGKTDDNTEDYIEYFQTLKLGYNDYVVRKGKKLSTICANDKTFESD
                     FKEYLNAFDTETRHLLGVDKSNDEEKYLDISGVCGILRAKKILFSIVQGWSKIDLEPF
                     DNSEITTLEEHIKRKWADISAETAGEQ"
BASE COUNT          833 a          370 c          429 g          813 t
ORIGIN      
        1 gtttttcatt aatcctcgtg tatgtgcatc atactgaaaa ttaggtattt cttgtctttt
       61 tgcatctagc tctgaaggag tgtcgaacgc gatataagct tttcctgcat ccagcaattg
      121 atccacatat ttactatata tctctttgcg ttcgctttgg cgacatgtgg cctgtccatt
      181 ctcaattccc tcgtcaaatt ttatgcccaa ccagcttagt gcttcaatta tatatttttc
      241 tgctccgggt acaaaacgtt gcgagtctgt atcttcaata cgtaaaatca tgcttcctgc
      301 atgctttttt gcgaataaat agttataaag cgcagtccta actccaccta tatgtagtgc
      361 tcctgtaggg cttggcgcaa atcttacgcg tatttttgta ttatccataa ttgtaatgtg
      421 tttcaagtgc caaagatagt gatttttggc ataattataa ttgttttgtg acttgagagt
      481 tttattctac atttgcgata atattgtagt actattatga aatcatctaa acaaaaaatc
      541 attttgatag cgcagatact actaattgtt tctacgcaat taatgatatc gcaaactaaa
      601 gctgttgtgg ttgataaaac aaccaaacaa ccaatccctt atgtaagtat ttatacaaaa
      661 aatgctgact tggttttagg ttctatgagt aatgagcaag gtcaattcga aatagatttt
      721 tcttttaata cacttttttt gtcgcatatc aactatggaa aagttgaaat aaaaaaagag
      781 aatgttcaag acacgatttt tttaatacct acttcagttt tattaaacga agtagttgtg
      841 aataaacgac aacccaagtg gataaatgct gtattatcta gagtaatcaa gggaaaaggg
      901 aaaaactatc aaacattaga aaagactttt tcatataagt atgaaacata tactttgaca
      961 gattccaatg gctatgaatt taaaagtaaa ggaaatattg aatttccaaa gatgaaaaaa
     1021 gatgttcaat ttaagattga tgctcaaagt aatacgatta aatacaagaa taaaactgcc
     1081 ggtgtagatt tctcaaattt aaggcggatg ttatatgatg attttgtaaa cggattcgat
     1141 aacaaatttt taaatgaata tgatttcagt caaaatagtt cttttgtggg cgaggataaa
     1201 aatgtaattc aattgatatt tagctcaaaa aagtttcagg atgatgaggg ttatattgtt
     1261 ttagatactt taaacaatgt tattcttgaa gctgaaaaga attcaggaac tgaatgtaat
     1321 atcaaaacaa aaacttcttc aatacttaga aatgtggttg catccagggg ttttaattat
     1381 gatacttgga ttacaaaaag caatacaaaa tactctaaaa taggagattg ctattatata
     1441 tccgagtgca aatataaatt ttacatgaag tcggtttaca aaaacaaaaa agttgattct
     1501 gaatatttta cttcaataga gtcgaaatta tttctttcag ataaaattga aagggcaagc
     1561 acttcattta agattctccc aaaaccttat tatatagttg ctatcatgac aaaaaagatg
     1621 agattggagg aggaggcttt aaacaaagtt cctgttagtt ttgaaggttt ttgagagtga
     1681 gggggaatat gtaattcttg aatctgcatg gcattctcag ttttattgtt tgattaattt
     1741 actcgtttat gggcaaaaaa cataaatgag aactaccgct tctaataaaa ataaacacaa
     1801 tattaggtgc atctttttac attcttttta aaatcacatt cttaaataat ttttgtactt
     1861 ttgtgactaa acaagtctaa aaatatgcca ttaaatattc tacaatacga gtccgacatt
     1921 tggtcgtcag ccgattccct tcgctctgcc ggtcttaaac aaagtgattt tcctaaatat
     1981 atgatgcctt tctttgcact ccgtatggtg gaaagccgtt tgatacgcga gcataaacgc
     2041 ctatctgctg agtttgggaa aactgacgat aatacagagg actatattga atacttccaa
     2101 acgttgaaac ttggttataa cgattatgtg gttcgtaagg gtaaaaagct aagcacaatt
     2161 tgtgccaatg ataaaacttt cgaatctgac tttaaagaat atttaaatgc ttttgatact
     2221 gaaactcgcc acttgttggg tgtggataaa agcaatgatg aagaaaaata cctcgatatt
     2281 tcaggagttt gtggcattct cagggctaaa aagattctgt tctctatcgt acaagggtgg
     2341 agcaaaatag atttagaacc tttcgataac tctgaaataa caactcttga ggaacatatc
     2401 aaacgcaaat gggctgatat ttcggcagaa acagcggggg aacaa
//
LOCUS       DAOPID010000218         2436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_60940_length_2436_cov_3.706426, whole genome shotgun sequence.
ACCESSION   DAOPID010000218 DAOPID010000000
VERSION     DAOPID010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60940_length_2436_cov_3.706426"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1364)
                     /gene="gldM"
                     /locus_tag="PK641_06565"
     CDS             complement(<1..1364)
                     /gene="gldM"
                     /locus_tag="PK641_06565"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HPG56041.1"
                     /translation="MMYLVLTAMLALNVSTDVLNGFTLVQKSLSQSLASTTAKNQSLY
                     GQFEDLKSQNPEKVAEWLNKANIVRQKTDSMNEVLEQLKYDIIVKADGQEGGDLYKEC
                     IKNGTQYAIGARDNLDASAQVAMAVSMQGASANRANQLKVGLNKYAALMEQMVQKDST
                     RIKTIRDNFNTEDTKTQNGDPITWEASRFEMIPVSASITLLTKIQTDLRYTESEVVNY
                     LKNQVDASDFRVNKITAEVIPTSSYVTRGGVYEARIILAAIDSTKRPKITVNGKVLEN
                     EKYTVGCSSVGTFDIKGQIDLPRADGTIQSYPFESSYIVGEPTAIISADMMNVFYAGI
                     ENPMSVSVPGVPTQNVSVSITNGILTRKGSGWTVRPTKVGQDCKISVSAKMEGGKVQS
                     IGSKPFRVKALPPPIGYIEYRENGNPAKYKGGKAFAKANLISSNGVKAELDDADLDVR
                     YNVLG"
     gene            complement(1448..2293)
                     /gene="gldL"
                     /locus_tag="PK641_06570"
     CDS             complement(1448..2293)
                     /gene="gldL"
                     /locus_tag="PK641_06570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HPG56042.1"
                     /translation="MAQGNFLTSPKGKKITGMAYGLGASVVIIGALFKIMHFPGAGPM
                     LMLGMGCEAILFALSSFEPPHKDYHWDMVFPQLEGGEGAPITGGGFGGATASAKQANS
                     IEDLAKAGSLSQSDVQRLNDGIRRLSETASQISDVTAAVSASSNYARNMNAASDAISA
                     FASTQNTLKASSDSLFASYKTVAESMTAVAGDAKGYINEMQGIAKNLSSINAGYEMQV
                     RSINTQAELMEGVNQNLSKMQNAMDSASAETEAFKAQATKLTQQVSSLNNVYGNMLNA
                     FNARV"
BASE COUNT          649 a          477 c          429 g          881 t
ORIGIN      
        1 ccgagtacat tatagcgaac atctaagtct gcatcatcaa gttctgcttt gacaccattt
       61 gaacttatta aattagcttt tgcgaatgct tttccacctt tgtattttgc aggattgcca
      121 ttttcacgat attctatata accaattgga ggtggtagtg cttttacacg gaaaggtttg
      181 cttcctatac tttgtacttt tccaccttcc attttcgcag atactgaaat tttacaatct
      241 tgtcctactt ttgtaggacg aactgtccaa cctgagcctt tacgagttaa tataccattt
      301 gtaatgctaa cactcacatt ttgtgtaggt acaccaggta cagacacgct cattggattt
      361 tcgataccgg catagaatac attcatcata tcagcagaaa tgatagcagt aggctcacca
      421 actatgtatg aactttcaaa aggatatgat tgtattgttc cgtctgcacg aggtaagtca
      481 atttgacctt taatatcgaa agttccaaca gaactgcatc cgactgtgta tttctcattt
      541 tcaagaactt tgccgtttac tgttatttta ggacgttttg ttgagtcaat agctgcaaga
      601 atgattcttg cttcatatac tcctccacgt gttacataag atgaagtagg gataacttct
      661 gctgtaatct tattaacacg gaagtcacta gcatcaactt ggttctttaa ataattgaca
      721 acttcagatt ctgtatagcg taaatctgtt tgaatctttg tcaacagtgt gatagatgca
      781 gaaacaggaa tcatttcaaa tcttgatgct tcccacgtaa taggatcgcc attttgggtt
      841 tttgtatctt ctgtattaaa gttgtcacga atagttttta ttctggttga gtctttttga
      901 accatttgtt ccataagagc ggcgtacttg ttcaaaccaa cttttaattg atttgcacga
      961 ttagcacttg ctccttgcat tgaaactgcc atagcaacct gtgcgcttgc atcaagattg
     1021 tcacgagcac ctatagcata ttgcgtgccg ttttttatac attctttata caagtcaccg
     1081 ccttcttgtc catcagcttt aactataata tcatatttca attgttccaa gacctcgttc
     1141 atactatcag tcttttgacg gacaatattt gctttattta accactctgc aaccttttcg
     1201 gggttttgac tctttaagtc ttcgaattgt ccgtataaag attggttttt agcagtggta
     1261 cttgccaaac tctgacttaa acttttctgt actaaagtaa agccgttaag tacatcggta
     1321 gatacattca aagccaacat ggctgtaagt accaagtaca tcatcgatat cattttctgc
     1381 ctcggggttt ccgggcaatt tgtagcactc atatattatt ttgtattaac aattagcggt
     1441 ttgattatta aactcttgcg ttgaatgcgt taagcatatt tccgtaaacg ttgttcaaac
     1501 tgcttacttg ttgtgttaat tttgttgctt gtgctttgaa tgcttctgtt tctgcagatg
     1561 ctgaatccat tgcattttgc atctttgata ggttttgatt tacgccttcc attaattcgg
     1621 cttgtgtatt tatgcttcta acttgcattt catatccggc gttgattgat gaaagatttt
     1681 tagcaatacc ttgcatttcg ttaatataac ctttagcatc tccagctact gctgtcatgc
     1741 tttctgctac tgttttatat gatgcgaata aagaatcaga tgaggctttt aaagtatttt
     1801 gtgtagatgc aaatgctgat atagcatcag atgctgcatt catgttgcgt gcgtaattag
     1861 atgaagcaga tactgcagct gtaacatcag aaatctgaga tgctgtttca cttaagcgtc
     1921 taattccgtc gtttaatctt tgtacgtcac tttgtgataa tgatccagct tttgctaaat
     1981 cttcaattga atttgcttgt tttgcagaag cagttgcacc accaaatcct cctccggtta
     2041 taggagcacc ttctccaccc tctaattgtg gaaataccat atcccaatga taatctttat
     2101 gtggcggttc gaatgaagat aatgcgaaaa gtatagcctc acaacccata cctagcatca
     2161 acataggacc tgcaccgggg aagtgcataa ttttaaacaa tgctccgata attactacag
     2221 atgctcctaa tccataagcc atgcctgtga ttttcttccc tttaggagaa gtaagaaaat
     2281 ttccttgtgc catttttttt gattttttaa ttgttttgat ataaagttat ttgtcttagt
     2341 cttgataatt tgctccgatt atactacgta cacagcggaa gccaatgtat gatttagttt
     2401 ctgtctgata ttcgtaagta cgggttgcac attgta
//
LOCUS       DAOPID010000219         2435 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_60974_length_2435_cov_4.715966, whole genome shotgun sequence.
ACCESSION   DAOPID010000219 DAOPID010000000
VERSION     DAOPID010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60974_length_2435_cov_4.715966"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="PK641_06575"
     CDS             <1..813
                     /locus_tag="PK641_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG56043.1"
                     /translation="TISDSDAKAKEEALYRSTYAAFMKGDASTVRANLANAEKAYPDS
                     KLMPKFIFLDAISSGRIDGREVFVKRLSDLVSKYPDSEIAPLARDMIALSAQGKELKA
                     TLPVSTISERREAVVTEAEFAENIKKAGFQYNPEDSHSFIMIIEGTLKQKNQVVFEVA
                     SYNFTRFMIKDFDLDVRTLNDSVYSISVGGLENLDEAIWYQNALLSDPQVLSSLQNVK
                     YKAFAVSDDNYRSIFDKESVEKYLEFYQQNKLEIKEPDKIKELEQSSGFVNP"
     gene            complement(872..>2435)
                     /locus_tag="PK641_06580"
     CDS             complement(872..>2435)
                     /locus_tag="PK641_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPG56044.1"
                     /translation="EYMQKAKDMHNALVEAAAEHDEGLMDKYFSDGSLSEDEMRLGIR
                     KGLTTHDILPVFCVCAGKDMGVRRLMEFLGNVVPFVSDMPYPETTDGTKVAPDKNAST
                     NLYVFKTSVEPHIGEISYFKVMSGTVKEGDDLFNATRNSKERINQLFSVAGQTKEKVE
                     ELVAGDIGATVKLKNTRTANTLNDGASEKQFGFVHYPDWKYRRAIKAVNASDDAKLGE
                     VLNRMHEEDPTWNIEYSKELKQTLVLGQGEFHLKTLKWRIENNDKIPVVFSDPKIPYR
                     ETIRKAARADYRHKKQSGGAGQFGEVHLIVEPLIEGEAAKELYKFNGQEFKISVRDKQ
                     ITPLEWGGKLEFINSIVGGAIDARFLPAIQKGIMDCIEQGPLTGSYARDVRVIVYDGK
                     MHPVDSNEISFRLAGRHAFSEAFKNAGPKILEPVYDVEITVPSARMGDVISDLQGRRA
                     VIMGMGSEKGMEKINAKVPLKEMNNYSIALSSNTGGRASFNMKFSSYELVPAEVQDVL
                     LKEYADTQKDED"
BASE COUNT          733 a          474 c          413 g          815 t
ORIGIN      
        1 acaatatccg atagtgatgc aaaagcaaaa gaggaggctt tatataggtc aacttatgct
       61 gcatttatga aaggtgatgc atctactgtg cgagctaatc ttgcaaatgc agaaaaagca
      121 tatccggatt ctaagctaat gccaaaattc atattcttgg atgctattag ctcaggacga
      181 attgacggac gtgaagtttt tgtaaaacgt ttatccgatt tagtaagcaa atatcctgat
      241 agcgaaatag ctcctttggc acgcgatatg attgcattat cggctcaagg taaagaattg
      301 aaagcaacat tgccggtaag tacaattagt gagcgtcgcg aagctgttgt aacagaagca
      361 gaatttgcag agaacattaa aaaggccgga ttccaatata atcctgaaga ttctcacagt
      421 tttataatga taatagaagg aactttgaaa caaaaaaatc aagtagtttt tgaagttgca
      481 tcatataact ttacaaggtt tatgataaaa gattttgacc ttgatgttcg tacacttaat
      541 gatagcgtct attctatttc ggtaggagga ttagagaatc ttgatgaggc tatatggtat
      601 cagaatgcat tgttgagtga tccacaggta ttaagttcat tacagaacgt taaatacaag
      661 gcatttgccg tttcagatga taattatcgc tctattttcg ataaagaatc tgtagaaaag
      721 tatttggagt tctatcagca gaacaagcta gaaataaaag aacctgataa aattaaagaa
      781 ctagagcaat catcaggttt tgttaatcca taaataataa acgccaagtt tttagcttgg
      841 cgtttattgt taaagcatac tctaatttac atcaatcttc atccttttga gtatctgcgt
      901 attcctttag taaaacatcc tgtacttcag caggtactaa ttcgtaacta gaaaatttca
      961 tattgaatga tgctctacca ccggtatttg aacttagtgc aatagaataa ttattcattt
     1021 cttttagagg aaccttggca tttatttttt ccataccttt ttcgcttccc atacccataa
     1081 taacagcacg tctgccttgc aaatcgctta ttacatcacc catacgtgca gaaggtactg
     1141 tgatttctac atcataaacc ggttccaaaa ttttcggtcc ggcattctta aatgcttcac
     1201 taaatgcatg tctaccggct agtctgaatg aaatctcatt agaatcaacc gggtgcattt
     1261 tgccatcgta aacaattact ctaacatctc ttgcataaga accggtaaga ggaccttgtt
     1321 caatgcaatc cattatacct ttttgtatag caggtaagaa acgagcatca attgcaccac
     1381 ctactatgct attaataaat tccaatttac ctccccattc gagtggtgta atttgtttgt
     1441 ctctaacaga gattttaaat tcttgaccat taaatttata caattctttg gcagcttcac
     1501 cttctataag gggttcaaca ataagatgta cttcaccgaa ttgacctgca cctcccgatt
     1561 gtttcttatg acgataatct gcgcgtgctg ccttccttat agtttctcta tatggtattt
     1621 tcggatcaga aaatacaaca ggaatcttgt cgttattttc aatacgccat tttaaagttt
     1681 taaggtgaaa ttctccttgt ccaagcacta aggtttgttt caattctttt gagtattcga
     1741 tattccatgt ggggtcttcc tcgtgcatac gatttagtac ttcgcctaat tttgcatcat
     1801 cacttgcatt aactgccttt atagctcttc tatatttcca atcaggataa tgaacaaaac
     1861 caaattgttt ttctgaagcc ccatcgttaa gagtattagc tgttctggtg ttttttagtt
     1921 ttacagtggc tcctatatct cctgcaacta gttcctcaac tttctctttc gtttgaccgg
     1981 caacagaaaa taattgattt atacgttctt tcgagtttcg agtagcatta aataggtcat
     2041 caccctcttt tacagttcct gacatcacct tgaagtatga aatttcgccg atgtgtggct
     2101 caacagaggt tttgaaaaca tatagatttg ttgaggcatt tttatcaggt gcaactttag
     2161 ttccatccgt agtttccgga tatggcatat cgcttacaaa aggcactaca ttacctaaaa
     2221 attccatcaa acgtcttacg cccatatcct ttcctgcaca tacgcagaaa acaggtaaaa
     2281 tatcatgagt agttaatccc ttacgtatac ctaggcgcat ttcatcttcg ctcaaacttc
     2341 catcgctaaa gtatttgtcc attaatcctt catcatgttc tgcagcagct tcaactaatg
     2401 cattgtgcat gtcttttgct ttttgcatat attct
//
LOCUS       DAOPID010000220         2419 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_61526_length_2419_cov_3.252961, whole genome shotgun sequence.
ACCESSION   DAOPID010000220 DAOPID010000000
VERSION     DAOPID010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61526_length_2419_cov_3.252961"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PK641_06585"
     CDS             <1..302
                     /locus_tag="PK641_06585"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA dimethylallyltransferase"
                     /protein_id="HPG56045.1"
                     /translation="NERLQNGMIDEVKGLLDKGIKSEDLIYYGLEYKYLTQWIIGELS
                     YNEMVSQLEIAIHQFAKRQMTWFRGMEKRGFKIHWIDAMLPNADKVSIVMDLLQD"
     gene            complement(291..923)
                     /locus_tag="PK641_06590"
     CDS             complement(291..923)
                     /locus_tag="PK641_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPG56046.1"
                     /translation="MADNYLENKYENLIKNKTSMGTKKATAVSSLMLKNRSYRGYDQR
                     CVVTLKHLKHIVSVNDKIPSARNQQVLRFKLVDSNNADKVLNLIKLGSALSDLHLPIK
                     GTEPRAFIIICSIIDADKWTYTDLGISCQSMLLQAVEMGLNGICIGAFNKEKIKDEFN
                     LEAEPLLILAIGKGAEKIRIKSIEADKDHKYYRENGEHIVPKVKIDELIL"
     gene            complement(1028..1112)
                     /locus_tag="PK641_06595"
     tRNA            complement(1028..1112)
                     /locus_tag="PK641_06595"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1076..1078),aa:Ser,seq:tga)
     gene            1248..>2419
                     /locus_tag="PK641_06600"
     CDS             1248..>2419
                     /locus_tag="PK641_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG56047.1"
                     /translation="MKKTVRYIIEFLIGTPEYADMIGYTGDVSLWSKYKVVIVPSDFF
                     NDGVFGTNNSMPKLPLQKINEIPLLYGEPVIKKQNDIVILKADIIASSFFLLTRYEET
                     INKKRDIHGRFRGKDSLSYRAGFIDRCVVDEYGELLRSCLKLVGVEVKNLDQKFSCVM
                     THDADKIEQYQNLRGFVGGLIHNHFKDAFVSLFGGIEKDLLYTFPWMKSIESELPNLS
                     SKVFIRVPNNTSIFDKPYYSLSSNGIKRLFELYPNYGLHISYEAGLNPELIADEKQKL
                     ELAGACKITECRNHYLASREPEDMMALVNCGITDDYTMGYADVAGFRLGTCKPVKWIN
                     PRTCYLTTLSLHSLVVMDCTLDRTEYMNLNYDDAYKCCLKLINIVRRHNGEWVGLW"
BASE COUNT          799 a          355 c          454 g          811 t
ORIGIN      
        1 ttaatgagcg cttacaaaac ggaatgattg acgaagttaa gggtttattg gataaaggta
       61 ttaaatcaga ggatcttata tattacggtt tagaatataa atatctaaca caatggataa
      121 taggtgagtt aagttataac gaaatggtat cacaattaga aatagcaata catcaatttg
      181 caaagcgtca aatgacttgg tttagaggta tggaaaaacg tggatttaaa attcattgga
      241 ttgacgcaat gttaccaaat gcagataaag tttctattgt gatggattta ctacaagatt
      301 aactcgtcga tttttacttt aggaacaata tgctccccat tttctctgta atatttgtgg
      361 tctttgtctg cttcaattga cttgatacgt atcttttcag cacctttgcc tatagccaaa
      421 attaaaagag gttcagcttc taaattaaat tcatctttaa ttttttcttt attaaatgca
      481 ccgatgcata taccgtttaa tcccatttca acagcttgca gtagcatgct ttgacaagag
      541 atacccaaat cggtataagt ccatttatcg gcatcaataa tactgcatat tatgataaaa
      601 gcacgtggtt ccgttccttt tattggaaga tgtaaatcgg atagtgcgct acctaatttt
      661 atcagattca atactttatc tgcattattc gaatctacta acttaaaacg taggacttgt
      721 tgatttctgg ctgatggaat tttatcatta acagaaacaa tatgtttaag atgctttaaa
      781 gtaactacac aacgttggtc gtatcctcta taacttctat ttttgagcat caaacttgat
      841 acggcggtag ctttttttgt gcccatactt gtcttatttt taataagatt ttcgtatttg
      901 ttttctaaat aattatcagc catagttgaa atatgatttt ggcaaagtta caaaaagctt
      961 tgatagagta gatatatata aaaaaagaaa agccctgcaa gtataaattg cagagcttca
     1021 tcttatgcgg aaagggaggg attcgaaccc ccggtacctt gcggtacagc ggttttcaag
     1081 accgctgtaa tcgaccactc taccaccttt ccaatggttt ttgtgttcgt ttttaataaa
     1141 ccgcgacaaa ggtaaatgtt tttatttaaa ctagaaaatt tatttttgcc aaactaacta
     1201 aaaagtgcta tatttgtgat cacaagacag aaaaactaca ttaatttatg aaaaagacag
     1261 tccgttatat catagaattt ttgataggga cgcctgaata tgccgatatg attggctata
     1321 caggtgatgt gtctttatgg agtaaataca aagtagttat tgtcccatct gattttttta
     1381 atgatggtgt atttggaacc aacaattcaa tgccgaaatt accattgcaa aaaatcaatg
     1441 aaatacctct attgtatgga gaaccggtaa ttaaaaaaca gaatgatatt gtaatactta
     1501 aggcggatat tattgcttca agtttctttc tgttgactag atacgaagag acgataaata
     1561 aaaaaagaga tatacacgga cgttttagag gaaaagattc tttatcatat agagcaggtt
     1621 ttatagatag atgtgttgtt gatgaatatg gggagttgtt gcgctcttgt ttaaaacttg
     1681 tcggagtaga agtgaaaaac ttggatcaaa aattttcgtg tgttatgacg cacgatgcag
     1741 acaaaataga acaatatcaa aatttaagag gatttgtagg aggtctgatt cataatcatt
     1801 ttaaagacgc ttttgtttct ttattcggcg gaatagaaaa ggatttgctg tatacatttc
     1861 catggatgaa atcaatcgaa agtgagcttc ctaatttgtc gtcaaaagtg tttattagag
     1921 ttccgaataa tacttctata tttgataaac catactattc gctatcatca aacggcataa
     1981 aacgtttgtt tgaactatat ccgaattatg gcttacatat aagctacgaa gcgggtttaa
     2041 atcctgaatt aatagccgac gaaaagcaaa aattagaact tgcaggtgct tgtaagataa
     2101 ccgaatgtag aaatcactat ttagcatcgc gtgagccgga ggatatgatg gctttagtga
     2161 attgcggtat aacggatgac tatactatgg gttatgctga tgttgcaggt tttagattgg
     2221 gaacgtgtaa accggtaaaa tggattaatc ctcgtacttg ttatctaaca actttaagct
     2281 tacattcttt agtagttatg gattgtaccc ttgatcgtac tgaatatatg aatcttaact
     2341 atgatgatgc gtataaatgt tgtttaaaat taatcaatat tgtacgtagg cataatggtg
     2401 aatgggtagg actctggca
//
LOCUS       DAOPID010000221         2414 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_61718_length_2414_cov_3.816448, whole genome shotgun sequence.
ACCESSION   DAOPID010000221 DAOPID010000000
VERSION     DAOPID010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61718_length_2414_cov_3.816448"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..168
                     /locus_tag="PK641_06605"
     CDS             <1..168
                     /locus_tag="PK641_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HPG56048.1"
                     /translation="PTATIKSSPDNSGTDLFVLHEGTKLTIKSVLGDWSEITTEDGNT
                     GWILSKSIEKI"
     gene            173..907
                     /locus_tag="PK641_06610"
     CDS             173..907
                     /locus_tag="PK641_06610"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPG56049.1"
                     /translation="MSVDIFQSFFEFIREIEQWDKSCFLQLNALHNDFFDGFMFWITD
                     KFVWIPLYISIIYVIIKNKKRETLLIAIGIILTIVFADQFASGFCKPLFERLRPSHDP
                     SIDLLVHTVRGDKGDLYGFISSHAANTFGLAMFTALLFKNTWYSFMIFMWAILNSYSR
                     IYLGVHFPIDILFGALSGVFIAWMFYALYLLLKKKLPQYRYSIHKTNIRTNVDYPQKD
                     LRIVFLTMLTTLFFIMLFASKNIFAY"
     gene            1022..1273
                     /locus_tag="PK641_06615"
     CDS             1022..1273
                     /locus_tag="PK641_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPG56050.1"
                     /translation="MVKQMTFNELRKIKDSLPNGSIHKIAEQLGIQEETVRNYFGGHN
                     FKQGGSCGLHIEPGPDGGIVILDDTTILDVALKMIAEKA"
     gene            complement(1338..>2414)
                     /locus_tag="PK641_06620"
     CDS             complement(1338..>2414)
                     /locus_tag="PK641_06620"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HPG56051.1"
                     /translation="VVKAMENATRELSKIETFRGYGPETGYRFLIDKIIKYDYNTRGV
                     SLEPDEVFISDGAKSDVGNIGHIFGRDNIIAITDPVYPVYENAAIMGGRSGHLTEQNI
                     WSNIVYIPCTPENNFIPELPKEKVDIIYLCYPNNPTGVALNKIELKKWVDYALENQAI
                     IIYDGAYAAYIKEDDIPHSIYEIKGAKKCAIEIRSYSKTAGFTGLRCSYTIVPKDIIG
                     YTLMNEKIKVNKLWNRRATTYYNGTSYIVQRGAEALYSRKGFKQYQTNIDYYLTNASI
                     IRQEMIGAGYEVYGGVNAPHIWIKTPKNTPSWRFFQELLYEINVVCTPGAGFGQGGEG
                     FFRFTAFNTRENTIEAMNRIKKRI"
BASE COUNT          735 a          381 c          408 g          890 t
ORIGIN      
        1 cctaccgcaa caattaaaag ttcgcctgat aatagcggta cagatttatt tgtactacat
       61 gaaggaacta agttgactat taaatcagtt ttaggtgatt ggtcagagat tacaacagaa
      121 gatggtaata cgggatggat tttgtcaaaa tcaatagaga aaatatagta tcatgagtgt
      181 tgatattttt cagtcttttt ttgagtttat tcgtgagata gagcaatggg ataagagttg
      241 ttttttacaa ctaaatgcat tgcataatga cttctttgat gggtttatgt tctggataac
      301 ggataaattt gtttggatac ctttgtatat atcaattatt tacgttatta ttaagaataa
      361 aaaacgtgag actcttctaa tagcaatagg tattattttg acaattgttt ttgctgatca
      421 atttgcatcc ggattttgta agcccttatt cgaaagatta agaccatctc atgatccttc
      481 aattgatctt cttgttcata cagttagggg cgataagggc gatttatacg gatttatttc
      541 ttcacatgct gcaaatactt tcggcttagc aatgtttact gcattactat ttaagaatac
      601 atggtattct ttcatgattt ttatgtgggc aatattaaac agttattcaa gaatatattt
      661 aggtgtacat ttcccgattg atattttatt tggagcattg tccggagtgt ttatagcttg
      721 gatgttttat gcattatatt tattattgaa aaagaaatta ccccaatacc gatatagtat
      781 acataaaact aatatcagaa caaatgttga ttatccgcaa aaagatttac gtatagtttt
      841 tttaacgatg cttacaacat tgttttttat tatgctattt gcttcaaaga atatatttgc
      901 ctattgattt atataactga tattttttag cataaaaagt ttgcatatca taaaaaaaag
      961 tattaagttt attgcagttt aattttacaa acattattta ttaacttaat actactggat
     1021 tatggtaaag cagatgacat tcaacgagtt gagaaagatt aaagacagtc tgcccaatgg
     1081 cagtattcat aaaattgccg aacaacttgg cattcaagaa gaaactgtca gaaattattt
     1141 cggtgggcat aattttaaac aaggaggttc ttgcggatta cacatagaac cgggtcccga
     1201 cggtggaatc gttattttgg atgatacaac cattttggat gtagctttga aaatgatagc
     1261 agaaaaagct taggagaaaa aaacaaatgg atacttaatt aagtatccat tttgtttata
     1321 tttcatttga cgttttttta tattctcttc ttaatgcgat tcatggcttc tattgtattc
     1381 tctcttgtgt tgaaagcagt gaaacgaaag aagccttcac ctccttgtcc aaaaccggct
     1441 ccgggagtgc aaactacgtt tatttcataa agtaattctt ggaaaaacct ccaagaaggt
     1501 gtgtttttag gcgttttaat ccaaatatgt ggagcattta ctccgccgta aacttcatat
     1561 cctgcaccga tcatttcttg tcgtattatg ctagcgtttg ttagatagta atcaatgttt
     1621 gtttgatact gtttgaatcc cttacgagag tatagtgctt ctgcacctct ttgtacaatg
     1681 tatgatgtgc cattataata tgtagtagct cttcgattcc acaatttatt tactttaatt
     1741 ttctcgttca taagagtgta ccctattata tctttcggta caattgtata tgaacaacgc
     1801 aatccggtaa aacctgcggt ttttgaataa ctacgtattt cgatagcaca tttcttagct
     1861 cctttaattt cgtatattga gtgtggaata tcatcctctt tgatatatgc agcgtatgcg
     1921 ccgtcatata taattatagc ttgattttct aatgcataat caacccattt tttaagttct
     1981 attttgttta gagcaactcc tgtcggattg ttggggtagc acaaatatat aatatcaact
     2041 ttttcttttg gtaattctgg aataaaatta ttctcaggcg tacaaggtat atatacaata
     2101 ttgctccaaa tgttttgttc tgtcagatga ccggaacgtc ctcccattat agctgcattt
     2161 tcgtatacag ggtatacagg atcagttatt gcaatgatat tgtctcttcc aaatatatgt
     2221 ccgatattac ccacatcaga ttttgcacca tcgcttataa aaacttcgtc aggttctaaa
     2281 gagactccac gtgtgttata atcatattta attattttat caatcaagaa acgataacct
     2341 gtttccggtc catatcctct aaatgtttct atttttgata attcgcgagt agcattttcc
     2401 attgccttca ccac
//
LOCUS       DAOPID010000222         2412 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_61801_length_2412_cov_3.759440, whole genome shotgun sequence.
ACCESSION   DAOPID010000222 DAOPID010000000
VERSION     DAOPID010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61801_length_2412_cov_3.759440"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="PK641_06625"
     CDS             <1..259
                     /locus_tag="PK641_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG56052.1"
                     /translation="YTTKDWDLLSPYCGLSFFETLPANLGKATSHNMAYYKSYPENKY
                     RAKFYKLFSKEKLPLAKMVTISLRPSIRMRIINKIKRLLEL"
     gene            256..1341
                     /locus_tag="PK641_06630"
     CDS             256..1341
                     /locus_tag="PK641_06630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HPG56053.1"
                     /translation="MRIALLNLPIDTNYGGNLQRFALIKTLENMGYEVEHIQLRSQSA
                     VKPLLRRCLSFVKRVLLMTAGRKDVHLFEEQFMREERKKRMANTDYFYNTYIPHTNHI
                     FYNRSDIESFDWSKYDAFLVGSDQVWRPAYAAFIGVENYFFNFVKDDSKPKIAYAVSF
                     GEKNTVFSEALHSQLCALYSKFKAVSVRESIGVDILESNGMAKPKAIPVLDPTFLLTP
                     ENYGRVIKESKISEHNNKYVLSYFIVNTPEKDSILKAKAKELELDTVFMSTESSKDIS
                     IPQWLYNFNHADFIVTDSFHGTVFSILYNKPFKLLSNKLNDESRTANLLHMFDITDTT
                     NVDWDKVNSLIEKYRKESFNFLKSSLS"
     gene            1348..2397
                     /locus_tag="PK641_06635"
     CDS             1348..2397
                     /locus_tag="PK641_06635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPG56054.1"
                     /translation="MAKKLIYVGFPFKHHKGTHAGYYHIKDTGIYNEIIDCTHEIEFP
                     NKCSKNKLTWFFFRVYRKIVGEGTPITVLHCIFISLFSRNNIFHFIHSENTYKWLAKF
                     KGKTNKIVLTVHQPASWFENHPIWINRIKEADKIILLSTDEEEKFKQWTGKDNVDFIP
                     HGIFTDFYKPDINIPHERMILMVGNWLRDFETANKVFKILLKQDTNLHINVVTLPVNE
                     KYFDRNERLHFLHGISDEDLRDLYRRSSMLFLPIKEYTANNAVLEAAATGCRIVVATN
                     HANMSYFSEDQIIVLSLDVNIIVDELSKLLETPCDIIKQAELRDFVVNNYSWSSIGKR
                     TKNIVLCNINYFIQK"
BASE COUNT          857 a          328 c          426 g          801 t
ORIGIN      
        1 gtatacaaca aaagattggg atttgcttag tccgtattgc ggattatcat tttttgaaac
       61 gttgcctgca aatttgggaa aagcaactag ccataatatg gcatattata aatcatatcc
      121 ggaaaacaag tatagagcaa aattctataa acttttttct aaagaaaaac tccctttggc
      181 taaaatggta acaattagtt tgagaccatc catccggatg agaataatta acaaaataaa
      241 acgtttattg gaattatgag aatagctttg ttaaatctgc caattgatac taattatggc
      301 ggtaatctgc agcgttttgc tttaattaaa accctagaaa atatgggcta tgaggtagaa
      361 catattcagc ttagatcaca gtcagctgta aagccattat tgcgtagatg tctttctttt
      421 gtaaaaagag tcttattaat gactgcagga aggaaagatg ttcatttgtt tgaagaacaa
      481 tttatgcgtg aagagagaaa gaaaagaatg gcaaatacgg attatttcta taatacatat
      541 atacctcata caaatcacat cttttataat aggtctgata ttgagtcatt tgattggagc
      601 aaatacgatg catttttggt aggttctgac caagtttggc gtccggctta tgctgcgttt
      661 ataggagtag aaaattattt ctttaatttt gtaaaggatg atagtaagcc taaaattgct
      721 tatgcagtgt catttggtga gaaaaataca gttttctcag aagcactaca ttcgcaatta
      781 tgtgccttgt actcaaaatt caaggcagtg tctgttagag aaagtattgg agtagatatc
      841 ttggaaagta atggaatggc aaaaccaaag gcaattcctg ttttagaccc gactttctta
      901 ttaaccccag aaaactatgg tcgtgttatt aaagaatcaa agatcagtga gcataataac
      961 aaatatgtat taagctactt tattgtcaat acgcctgaga aagactctat actcaaagca
     1021 aaagcaaaag aattagaatt agatacagtt tttatgagca ctgaaagttc taaggatata
     1081 tcaatacctc aatggttata taattttaac catgcagatt ttattgtaac agattctttc
     1141 catggcacag tgttcagtat tctgtataat aagcctttta aattattaag taacaagtta
     1201 aacgacgaat cacgtacagc aaaccttttg catatgtttg acataacaga tactaccaat
     1261 gtagattggg ataaagttaa ttcgttgata gaaaaatata ggaaagaatc atttaatttt
     1321 ttaaaatcct cattaagtta attttgaatg gcaaagaaac tcatttatgt aggtttccca
     1381 tttaaacacc acaagggaac ccatgctgga tattatcata ttaaagatac tggtatttac
     1441 aatgaaatta tagattgtac tcatgaaatc gagtttccaa ataagtgctc taaaaataaa
     1501 cttacttggt tcttttttcg tgtctatcgc aaaattgtag gagaaggaac tccaattact
     1561 gttttgcatt gtatttttat tagtcttttc tctagaaata atattttcca tttcattcat
     1621 tctgagaata catataagtg gcttgcaaag tttaaaggaa agactaataa gatagtttta
     1681 actgtgcatc agcctgcatc atggtttgaa aatcatccta tatggattaa taggataaaa
     1741 gaggctgata aaataattct tttgtcaact gatgaagaag aaaaatttaa gcaatggaca
     1801 ggaaaggaca atgtggattt tattcctcat ggtatattta cagactttta taagccagat
     1861 ataaatatac cacatgagcg tatgatactt atggtcggta actggcttag agattttgaa
     1921 accgcaaata aggtctttaa gattttgttg aaacaagata ctaatttgca tattaatgtt
     1981 gttacattac cagtaaacga aaaatatttt gatagaaatg agagattaca ttttcttcat
     2041 ggcatatcag atgaggattt aagagactta tatagaagaa gttctatgtt atttttgcca
     2101 attaaagaat atacagctaa taatgccgta cttgaggctg ctgccacagg ttgtaggata
     2161 gttgtagcca caaatcatgc aaatatgtca tatttttctg aagatcaaat tatagtttta
     2221 tctttggatg tgaacattat tgtagatgaa ttgtctaaat tgcttgaaac gccatgtgat
     2281 attataaaac aagcagagct acgtgacttt gtagttaata attattcatg gagtagtatc
     2341 ggtaagagga caaaaaatat tgttttgtgt aacataaatt attttattca aaaatgaaaa
     2401 attgcctgtt at
//
LOCUS       DAOPID010000223         2404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_62102_length_2404_cov_2.931886, whole genome shotgun sequence.
ACCESSION   DAOPID010000223 DAOPID010000000
VERSION     DAOPID010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62102_length_2404_cov_2.931886"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2404)
                     /locus_tag="PK641_06640"
     CDS             complement(<1..>2404)
                     /locus_tag="PK641_06640"
                     /inference="COORDINATES: protein motif:HMM:NF013957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HPG56055.1"
                     /translation="NRAISRYGNSKSDSIEIVKIYDSIIRLNAYNREAAAYAEAQKAA
                     YLGQSFDNILNRYSDTQISVYITYLKCLKLPKKQAHEICVQTLKSNPNNKYNSSLQNL
                     LSSIEKPELSVNMNSLAYPGDSIKLKCSYVNINKLHCNIYRLNISVTDALKYRYKNIP
                     DSLKKLIGTVSYSLSKTDDYETKNVSLMMKGLDPGYYGIDLVADTCKSKPLSFNMMAV
                     SRFLLFKKQDGTNLSCYVTDRKTGKYIKNVSVDIYKANKIMHTYKTNSIGIAEINLPK
                     STNYYIGLSMGKDMYYPLDEIYTYERSKYYQSDIQTQFLLDRKIFRPGQTLYFKGISG
                     IADTAQFSLQTNKSIHVVLRDANYKIINESDFITNEFGTFSGTFKLPSNALQGAYYIQ
                     TNNGSETFRVESYKRPTFELKIEQTNTAYTFGDTMNIKGSAIYYAGYPLRKADVKYKL
                     VRQVMSFRTFYPILSRQETIEIGTTKLDENGMFNINAAIGGYNNSDFIDESRVGNFYR
                     YVIYVDVTDDAGETQSAEYSISIGKKSMCLNIDIPQFVNKSSQAIFTVYAKSLDGKEI
                     AVNGNYEILKDAQVIAKADLVGGKAVIIKDLSKLVSGKYLLKVSCSDKNGKTLTDEKS
                     FVIFGEKDKKPAVDTIMWFMPLNKSCKADEKFKFVIGSSEKTYILCEVYDAQKLLMRK
                     IIYLNNKNKTVTMPYKNNYGDNISVRVMSVKNASVYKDETNINKEQIDDKISISVESF
                     RDKLEPGDKESWTILIKDANGKPIKSEVLADMYDLSLDAFVSNNWTLNMDKRLKLSAA
                     MWS"
BASE COUNT          760 a          423 c          308 g          913 t
ORIGIN      
        1 gaccacatag ctgcagataa ttttaaccgt ttgtccatgt tcaatgtcca attattggac
       61 acgaatgcat ccaaagacaa atcatacata tcagctaaaa cctcagattt aatcggtttt
      121 ccattagcat ctttaataag tattgtccaa gattctttat cacccggctc taacttatct
      181 ctaaaacttt ctacggatat agatatctta tcatctattt gctccttatt tatatttgtt
      241 tcatccttgt aaactgaagc gtttttaaca gacataactc taacagaaat attatcacca
      301 taattatttt tatatggcat agtcactgtt ttatttttat tgttaagata tataatcttg
      361 cgcatcaata atttttgtgc atcatatacc tcgcatagta tatatgtttt ttctgatgag
      421 cctataacga acttaaattt ttcgtcggcc ttacacgatt tatttaaagg cataaaccac
      481 ataatcgtat caacagcagg ttttttatct ttttcaccaa atatcacaaa tgatttttca
      541 tctgtcaatg ttttaccatt tttatcagag catgatactt ttaataaata ctttccggaa
      601 acaagcttcg ataaatcttt tataatcaca gctttaccac ctactaaatc agcttttgca
      661 attacttgag catcttttaa aatttcataa ttaccattaa cagctatttc tttaccatct
      721 aatgatttag catatacagt aaatatcgct tgcgaactct tattcacgaa ctgcggtata
      781 tcaatattca gacacatcga ttttttgcca atcgatattg aatattctgc cgactgcgtt
      841 tcgcctgcat catcagtaac atctacataa ataacatatc tatagaaatt acctacacgc
      901 gactcatcta taaaatcact attattatac ccaccaatag ctgcgttaat attaaacatt
      961 ccattctcat caagtttagt cgtacctatt tcaatagttt cttgacgact taagattggg
     1021 tagaaggttc taaaactcat tacttgccta acaagtttat atttaacatc ggcttttctg
     1081 agtggatatc ctgcataata aatagccgag cctttaatgt tcattgtatc accaaatgtg
     1141 tacgctgtat tagtttgctc tatctttaat tcaaatgttg gacgtttgta tgattctact
     1201 ctaaatgttt ccgatccatt gttagtttga atatagtatg ctccttgcaa agcatttgaa
     1261 ggtaatttaa acgtacctga gaatgttcca aactcattgg taatgaaatc actttcattt
     1321 attatcttat aatttgcatc tcttaaaaca acatggatgg atttatttgt ttgcaaacta
     1381 aattgtgcag tatccgcaat accggaaatt cctttaaaat acaatgtttg acccggacgg
     1441 aatatctttc tatctaacaa gaactgcgtt tgaatatctg attggtaata ttttgacctt
     1501 tcgtaagtat atatttcatc taaaggatag tacatatctt tacccataga taatcctatg
     1561 taataattgg tagatttagg aagatttatt tctgcaatac ctattgagtt tgttttataa
     1621 gtatgcataa ttttatttgc tttataaata tcaacactta catttttgat gtatttacct
     1681 gtttttctgt ctgttacata acaagataaa tttgttccgt cttgtttctt gaaaagaaga
     1741 aaacgtgata ctgccatcat attaaatgat aatggctttg atttacaagt atcagcaacc
     1801 aaatcaatac catagtaacc cggatccaat cctttcatca ttaacgatac attctttgtt
     1861 tcataatcat cggttttaga caaactatac gaaacggtac caatcagttt ttttaatgaa
     1921 tcaggaatgt ttttatagcg atactttaat gcatctgtaa ctgatatatt taatctatat
     1981 atattacaat gcaatttatt gatattcaca taagaacatt tcaatttaat cgaatcaccg
     2041 ggatatgcca aagaattcat atttacagac aactccggtt tttctataga tgataataaa
     2101 ttctgtaaag aactgttgta cttattatta ggattcgatt ttaaagtttg cacacatatt
     2161 tcatgtgctt gtttctttgg taattttaga catttaagat atgtaatata aacagatatt
     2221 tgcgtatctg aatagcgatt caagatatta tcaaatgatt gccctaaata agctgctttt
     2281 tgagcttcgg cataagctgc tgcctctctg ttataagcat ttaatcttat tattgaatca
     2341 taaattttga ctatttctat actatctgat ttactatttc catatctact aattgctcta
     2401 tttg
//
LOCUS       DAOPID010000224         2399 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_62299_length_2399_cov_2.983788, whole genome shotgun sequence.
ACCESSION   DAOPID010000224 DAOPID010000000
VERSION     DAOPID010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62299_length_2399_cov_2.983788"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..1493
                     /locus_tag="PK641_06645"
     CDS             162..1493
                     /locus_tag="PK641_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG56056.1"
                     /translation="MKNIILYINLIIVTIIGFSSCSKQNATQDRTAAEIINNPSYTAM
                     SFGGYRHLTRDTVPSVTDLKEDVRILSAMGIKLIRLYDTQLGQTPNLLQAISELKQED
                     KSLELYVMLGAWIEAENSRTDSVNHHKENIIGNTAEIEKAVEYTNKYPDIVKCISVGN
                     EAMVHWAASYNVTQDVILKWVKYLQALKQEDKLPKDVWITSSDNFASWGGGDGSYHTD
                     LLLELYKSVDFISVHTYPFHDTFYNPSYWYVPEEEANLDAIVQIDRAMERAVAYTKMQ
                     YMSVVNYMHSIGINKPVVIGETGWASLDGAYSPSADASADISINIQKIINKEQTMYGL
                     NGSKAADEYKEKLYYDRLREWTAKDSITCFYFEIFDEPWKDAKHPSGTENHFGLIDIN
                     GHAKYALWNLVDDGTFKGLKRNGKAISKSFNGNRESLMKTVLPPPLITTKE"
     gene            complement(1534..>2399)
                     /locus_tag="PK641_06650"
     CDS             complement(1534..>2399)
                     /locus_tag="PK641_06650"
                     /inference="COORDINATES: protein motif:HMM:NF039873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG56057.1"
                     /translation="RINAKGEYYFTPYPYGTSNFSLGVDYNGINIYEYGKRACNLTYV
                     KPSGSFYFSDQSVADHDFRICAGVNVEYYDYTDTLYTDGSERLNLIQDGLMFNYFAKI
                     NYDTFDRKYFPKYGTELDFTATLHTDNTYAYAGGNPMLSVMAYWQSVFPCNSYFAIVP
                     SVYGRFIFMTDGRPVPFHISNIMGGYTPGRYYAQQLLFEALSHLETFENYLLATGLGF
                     RGCILKRHYITLTGNFAMTNDYIFSMHKEMIYGGALRYSYDSIVGPLTLSMGYTNRSR
                     FGIYLHVGFMF"
BASE COUNT          829 a          398 c          426 g          746 t
ORIGIN      
        1 cagaagcaaa tatcaatgca ggtattgctg ccgctaacca aattgttgga ttcttgaaag
       61 atggtatcga gaaattccgc gtaaacaaat aattttatat tattctgcat aaaagccgaa
      121 gccatataac ttcggctttt tttaccttaa tactcattgc aatgaaaaat attattttat
      181 acatcaatct aatcatagta actataatcg gatttagctc ttgttcaaaa caaaatgcaa
      241 cacaagatcg cactgctgcc gaaataataa acaatccgag ctacactgct atgtcgtttg
      301 gaggatatag acatttaact agagatacag tgccttctgt tactgattta aaagaagatg
      361 tacgcatttt gtctgctatg ggtataaaat taatacgctt atatgatacc caattaggac
      421 aaacgcctaa cttattacaa gcaataagtg aattaaaaca agaggataaa agtttggaac
      481 tctatgttat gttaggagct tggattgaag cagaaaattc cagaacagac tcggtaaacc
      541 accacaaaga aaatattata ggtaatactg ccgaaataga aaaagctgtt gaatacacta
      601 ataaatatcc tgatattgtt aaatgtattt ctgttggtaa tgaagctatg gttcactggg
      661 ctgcaagtta taatgttaca caagacgtca ttttaaaatg ggttaaatat ctgcaagcat
      721 taaaacaaga agataaatta ccaaaagatg tatggattac atcatcagac aattttgcat
      781 catggggagg tggcgatggt agttatcata cagatttgct tttggaatta tacaaatcgg
      841 tcgattttat ttctgtacat acctatcctt ttcatgatac attttataac ccctcatatt
      901 ggtacgttcc tgaagaggaa gctaatttag atgctattgt acaaattgac agagcaatgg
      961 aacgtgctgt tgcatacact aaaatgcaat atatgtcggt tgttaattat atgcattcaa
     1021 ttggtattaa taaacctgta gttattggag aaaccggttg ggcaagccta gatggtgcat
     1081 atagccctag tgctgatgca tcagcagata ttagtataaa tattcaaaaa atcattaata
     1141 aagagcaaac aatgtacgga ttaaatggtt cgaaagcggc tgacgagtat aaagagaaat
     1201 tatactatga cagattacgc gaatggacag ctaaagacag tatcacttgt ttttattttg
     1261 agatatttga cgaaccttgg aaagatgcta agcatcctag cggaacagag aatcacttcg
     1321 gattaattga cattaacggg catgcaaaat atgctttatg gaatcttgtt gatgacggta
     1381 cttttaaagg actaaaacgt aatggaaaag ctattagcaa atcttttaat ggcaatcgag
     1441 aatcactaat gaaaactgtt ttacctcctc ctcttattac aactaaggag taattataaa
     1501 ataaaaagag agccgtaaag ctctcttttg tttttagaac ataaatccaa catgaagata
     1561 tattccaaac ctcgatctgt tagtatatcc cattgataaa gttaatggcc ctactatgct
     1621 atcgtaagaa tatcttaatg ctccaccata tatcatttct ttgtgcattg agaagatata
     1681 atcattagtc attgcaaagt ttccggtaag cgttatgtag tgccttttta atatgcatcc
     1741 cctaaaacct aaaccggtag caagaagata gttttcaaat gtttctaagt gtgacagtgc
     1801 ttcgaaaagc agttgctgcg cataatatcg cccgggcgtg tatcctccca taatatttga
     1861 aatgtggaat ggaacgggac gtccgtctgt cataaaaata aatcgtccat acactgatgg
     1921 tactattgca aaatagctgt tacacggaaa tacagattgc caatatgcca taacagacaa
     1981 catcggatta ccaccggcat atgcataggt attatcggta tgtaatgtag ctgtaaaatc
     2041 taattcggtt ccatatttgg ggaaatactt acgatcaaat gtatcataat taatttttgc
     2101 aaaataatta aacattaaac catcttgaat caaatttaaa cgttcagatc catctgtata
     2161 taatgtatca gtataatcat agtactcgac atttacccct gcacaaattc taaagtcatg
     2221 atcggcaact gattgatctg aaaaatagaa tgatcctgat ggttttacgt atgtcaggtt
     2281 acatgctctt ttcccgtatt cgtatatgtt tatgccatta taatcaacac ctagtgaaaa
     2341 atttgatgtt ccgtatgggt atggtgtgaa atagtattca ccttttgcat ttattcgtc
//
LOCUS       DAOPID010000225         2386 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_62755_length_2386_cov_3.739597, whole genome shotgun sequence.
ACCESSION   DAOPID010000225 DAOPID010000000
VERSION     DAOPID010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62755_length_2386_cov_3.739597"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="PK641_06655"
     CDS             complement(<1..811)
                     /locus_tag="PK641_06655"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HPG56058.1"
                     /translation="MSDNLKEKMIGALTWSSIDRIGQQGVQFIIGIVLARLLCPADYG
                     LIGMVMIFAQFAYAMVESGLGAALVRTKNLTETHCNTVFYTNLSISIILYTILFFCAP
                     MLADFFNQPILTIISRVTFAAILFNSMYLVPFNIAGRELDYKTITKVNITSTIISGTA
                     GITMAYYNMGVWALVVQQTGYHFVRMICFHAFSKWKPKLLFSFKILGEYFQFSSHMLG
                     TSLLTVTFNNIYTFLLGKYYSVNQVGFYTQGYKLSDSVNFTFIAILSTSYNL"
     gene            complement(827..>2386)
                     /locus_tag="PK641_06660"
     CDS             complement(827..>2386)
                     /locus_tag="PK641_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPG56059.1"
                     /translation="RDYAFAKRFNLPIIPLIEGCDISEESYDAKEGIMMNSGFMTGLS
                     VKEAITTAKKYITEKGIGKVKVNYRLRDAIFSRQRYWGEPFPIYYKDGQPYTLDETCL
                     PLELPEIDKYVPTETGEPPLGRAKNWQTKDGYPLELNTMPGFAGSSAYYLRYMDPHND
                     NELVNKDVNLYWKNVDLYIGGTEHATGHLIYSRFWNKFLFDLGLICKDEPFNKLINQG
                     MIQGRSNFVYRIIGTNKYVSYNLKDKYETQEIHVNVNIVSNDILDIEAFKNWMPEYKD
                     AEFILEDGKYICGWAVEKMSKSMFNVVNPDDIINKYGADTLRLYEMFLGPLEQSKPWD
                     TNGIDGVHRFLKKFWGLFFNGDSVVISDVEPTAEELKSLHKTIKKITSDIENFSYNTS
                     VSAFMICVNELSQMKCNKRAILEPLTILIAPFAPHIAEEMYNMLGYKTSVCNAKYPEY
                     DEKFLIESKVKYPISFNGKVRFTIELPADTDKTEVERIALSNNQTEKYTEGKTPKKVI
                     VVPGKIVNIVL"
BASE COUNT          761 a          425 c          371 g          829 t
ORIGIN      
        1 aaagattgta actggtgctt aatattgcta taaaagtaaa attgacacta tcacttaatt
       61 tatatccttg agtgtaaaaa cctacttgat ttacagaata atatttacct aaaaggaagg
      121 tatatatatt attaaaagta actgtaagta aagaggtacc aagcatgtgt gaactaaatt
      181 ggaaatattc gcccaatatc ttaaaagaga aaagcaattt aggtttccat ttactaaatg
      241 cgtgaaaaca tatcattcta acaaaatgat aacccgtttg ttgtacaact aaagcccata
      301 cacccatgtt gtaatatgcc atagtaatac cggctgttcc tgaaataatg gtacttgtaa
      361 tgtttacttt ggtaattgtt ttataatcta gttcgcgtcc ggcaatatta aatggtacca
      421 gatacattga attaaataat atggctgcaa atgttacacg cgaaatgatt gtcagtattg
      481 gctgattaaa aaaatcagca agcataggtg cgcaaaaaaa caggatagta tataatatta
      541 ttgaaatgct tagatttgtg tagaatactg tgttgcaatg agtttctgtt agatttttag
      601 tacgcacaag tgcagctcct aaaccacttt cgaccattgc gtaagcaaat tgagcaaaaa
      661 tcataaccat accgattagt ccgtaatcgg cagggcacaa taatcgagcc aatacaatcc
      721 caattataaa ttgcactcct tgttgtccaa ttctatcaat tgaactccaa gttagagcac
      781 ctatcatttt ttcttttaaa ttgtcgctca taattggcga aatcttttac aatactatat
      841 tgacaatctt tccgggaaca acgattactt ttttaggagt tttaccctcg gtgtattttt
      901 ctgtttgatt attagataag gctattcgtt caacttctgt tttatctgtg tcagccggta
      961 attctatggt aaaacgaact tttccgttaa atgagatagg gtatttgacc tttgattcaa
     1021 tcaagaactt ttcatcatat tcaggatatt tagcattaca aacacttgtc ttatatccaa
     1081 gcatattgta catctcttct gctatatgtg gagcaaatgg agcgataagt attgtaagcg
     1141 gttctagaat tgctctctta ttgcatttca tttgcgataa ctcattgacg caaatcataa
     1201 atgcagatac agaagtatta tatgagaaat tttctatatc agatgtaatc ttcttaattg
     1261 ttttatgtaa tgatttaagt tcttctgcag tgggttcaac atcacttata actacagaat
     1321 caccattaaa gaataatccc cagaatttct ttagaaatct atgaactcca tcaataccat
     1381 ttgtgtccca tggtttagat tgttctagtg ggcctaaaaa catttcgtat aaacgtaagg
     1441 tgtcagcacc atacttgttt ataatatcgt caggattcac tacattgaac attgatttgc
     1501 tcattttttc tacagcccaa ccgcaaatgt atttaccgtc ttctaaaata aattctgcat
     1561 ccttgtactc cggcatccaa tttttgaatg cttcaatgtc aagaatatca tttgaaacga
     1621 tgtttacatt tacatgtatc tcttgggttt catatttgtc tttcaaatta tatgatacat
     1681 atttgttggt tccaattatg cgatatacaa aattagaacg tccttgtatc ataccctgat
     1741 taattaattt attgaatggt tcatctttac atattaaacc caaatcaaat aaaaatttat
     1801 tccagaaacg gctgtatatt aggtgtcctg ttgcatgctc cgtacctccg atatacaaat
     1861 caacattttt ccaatataaa tttacatctt tgttaaccaa ttcattatca ttatgaggat
     1921 ccatgtacct aagatagtat gccgatgaac ctgcaaaacc gggcattgtg ttcaattcta
     1981 aaggataacc gtcttttgtc tgccaatttt tagcacgacc aagtggaggt tcacccgttt
     2041 cggtaggaac atacttgtct atttcaggta attcaagagg caaacaagtt tcgtctaatg
     2101 tgtagggctg tccatcctta taataaatag ggaatggttc gccccaataa cgctgacgac
     2161 taaatattgc atcacgtaat cggtaattta cttttacttt accaatacct ttttctgtaa
     2221 tgtatttctt ggcagttgtt attgcttctt ttacagagag tcctgtcata aatcctgaat
     2281 tcatcatgat accttctttt gcatcataac tttcttcact tatatcgcaa ccttcaatta
     2341 aaggaattat aggcaaatta aagcgttttg caaaagcata gtcacg
//
LOCUS       DAOPID010000226         2341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_64430_length_2341_cov_3.774278, whole genome shotgun sequence.
ACCESSION   DAOPID010000226 DAOPID010000000
VERSION     DAOPID010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64430_length_2341_cov_3.774278"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PK641_06665"
     CDS             complement(<1..310)
                     /locus_tag="PK641_06665"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HPG56060.1"
                     /translation="MKYSDNLSEIIKYSQEEAERLGNNYIIPEHLLLAIIRAGEGNAF
                     NLLIALNLNLTELKKLIESNIRTNNNVMGQVLPYLQSTEKILKYVVLEARMQHCDTAD
                     S"
     gene            520..>2341
                     /gene="gyrA"
                     /locus_tag="PK641_06670"
     CDS             520..>2341
                     /gene="gyrA"
                     /locus_tag="PK641_06670"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPG56061.1"
                     /translation="MTEEERIIKVDIEEEMRTAYIDYSMSVIVSRALPDARDGLKPVQ
                     RRVLYGMELLNNTYDKQTKKSARIVGEVMGKFHPHGDSSIYGTLVHMVQSWAMRYPLI
                     YGQGNFGSVDGDSPAAMRYTEAKLQRLAEEMLADIDKNTVDMVKNFDETLDEPTVLPG
                     LLPNLLVNGTTGIAVGMATNMAPHNLSDTVDAIVAYIDNNAIEDDDLISILKAPDFPT
                     GGILYGYSGIREAYLTGRGRVVIRARVEIETEADQERLIITELPYMVNKSELQKHIAL
                     LVNEKRIEGISNIADESNHEGMRLIIDIKRDANANVVLNKLYKETALQSSFSINNIAL
                     VKGRPRLLCLRELIDCYVQHRHEIITRRVSFDLKKAQDRLHIVEGLIIASDNIDEVIA
                     IIKAAHTVGDAIEKLIERFSLSEIQAKAIVEMRLRQLTGLSQDELHAERDELMLKIED
                     LNDILSRIERRMGIIKDELLSLKEKYGDERRSEIVYASEEFNPEDFYADDEMIITISH
                     LGYIKRTPLSEYKSQNRGGVGSKGSDSRDEDFIEYIYPASMHSYMLFFTQKGRCYWLK
                     VYEIPEGSKNSKGRAIQNMLNIEKEDKVNAFIKVKQLQDPE"
BASE COUNT          787 a          339 c          456 g          759 t
ORIGIN      
        1 cagaatcagc agtatcacaa tgttgcatac gagcttctaa aacaacgtat tttaatattt
       61 tttcggttga ttgcagatat ggtaatacct gacccattac attgttattg gtgcgtatat
      121 tggattcgat tagcttttta agctctgtta gattaagatt aagtgcaatc aacaaattaa
      181 atgcattacc ctcaccggca cgtattatgg ctaacaataa gtgttcagga ataatgtaat
      241 tattacctaa tcgttctgct tcttcttggc tatatttaat gatctcagat aaattatctg
      301 aatatttcat gttcatagtt ctattttttt acaaagttat aatttttgac taattatttg
      361 acatttatag tgtctcattt tatcaatagc aaataatatg ccaaaaataa tatgattgtt
      421 aataagattt tcgacttgaa ttttggttaa ttcggcaaaa ggcagtaact tagcgtgtct
      481 ttttgaagat agtagataat agcaaaaata aatttataaa tgactgaaga agagagaatt
      541 ataaaagttg atattgaaga agagatgcgg actgcctaca tcgattattc aatgtcagta
      601 atagtgtctc gtgcattacc ggatgcaaga gatggtttga aacctgtaca acgtcgtgtt
      661 ttatatggta tggaattact taataacaca tacgacaaac agactaagaa atcagccaga
      721 atagtaggtg aagttatggg taagtttcat ccacacggcg attcctcaat ttatggaact
      781 ttggttcata tggtacaaag ttgggcaatg cgttatcctt tgatatatgg acaaggaaac
      841 tttggttctg ttgatggaga ttcacctgct gcaatgcgtt atactgaggc taaattacaa
      901 cgtttggcag aagaaatgtt agctgacatt gacaagaata cagttgacat ggtcaaaaac
      961 tttgacgaaa ccttggatga accaactgta ttacccggtc ttcttcctaa tcttttagtt
     1021 aatggtacaa cgggtatagc tgtaggtatg gctacaaata tggctcccca taatttgtcg
     1081 gatactgttg atgcgattgt tgcttacatt gataataatg caattgaaga cgatgattta
     1141 atctcaatat taaaagctcc tgattttcca accggaggta tcttatatgg ttattcgggt
     1201 attcgtgaag catatctgac aggtcgtggt cgtgtagtga ttagagcgcg tgttgaaatt
     1261 gaaacagaag cagatcaaga gcgtttgata atcactgagt taccatacat ggttaacaaa
     1321 tcagaattac aaaaacatat tgcattactt gtaaacgaaa aacgtataga gggtatttca
     1381 aatattgctg atgaaagtaa ccacgaaggt atgcgtttaa ttattgatat taaacgtgat
     1441 gctaatgcta atgtagttct taacaaatta tataaggaaa ctgcactaca atcatcattt
     1501 agtattaaca atatagcctt ggtaaaaggt cgtccacgtt tgttatgctt gcgcgaactt
     1561 attgattgct atgtacaaca tcgtcatgag ataataacta gaagggtaag ctttgatttg
     1621 aaaaaggctc aagaccgttt acatattgtt gaaggtttaa taatagcatc agacaatatt
     1681 gacgaagtta ttgctattat aaaagcagct cacacagttg gtgatgccat tgaaaaatta
     1741 atcgagcgtt tctctctatc agaaattcaa gctaaggcaa tcgtagaaat gcgtttgcgt
     1801 caattaacag gattatctca ggatgagtta catgctgaac gtgatgaatt gatgcttaag
     1861 atagaggatt tgaatgatat tttatctcgt attgaacgca gaatgggaat aattaaagat
     1921 gaattgctct cattaaaaga aaaatacgga gatgaacgtc gttctgaaat tgtatatgca
     1981 tcagaagaat ttaatcctga agatttttat gctgatgacg aaatgattat tactatttca
     2041 catcttggat atattaaacg tactccatta agtgagtata aatctcaaaa ccgtggtggt
     2101 gtaggatcta agggtagtga tagccgtgat gaagacttta ttgagtatat atatcctgca
     2161 tcaatgcata gttatatgtt attctttact caaaaaggac gttgctattg gcttaaagtg
     2221 tacgaaatac ccgaaggatc taagaattca aagggtcgtg ctatacaaaa catgcttaat
     2281 attgagaaag aagacaaagt caatgcattt attaaggtta aacaattaca ggatcctgag
     2341 t
//
LOCUS       DAOPID010000227         2316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_65453_length_2316_cov_3.458647, whole genome shotgun sequence.
ACCESSION   DAOPID010000227 DAOPID010000000
VERSION     DAOPID010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65453_length_2316_cov_3.458647"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="PK641_06675"
     CDS             complement(<1..1008)
                     /locus_tag="PK641_06675"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG56062.1"
                     /translation="MNLPLYIAGRYLFSKKSHHAINIVSLISVCGVAVGTMALVCVLS
                     VFNGFQGIVEGLFGNFDPQLKITAKQVKVFDPVFVSKISEMPDVEYCREVLQENALLV
                     FRDKQTPVVVKGVPDDYAKINHISDIILDGDFKTKEGSFDMAVGGIGLVRTIGCGVHY
                     VEPLQLYAPKRSGNVNMIRPDEAFKREIAFLTGIFMVQQEKYDNNLLIVSMSLARRLF
                     DYEKEVSAVEIKLKDGANEKKVQASISKMLGDDFSVQNRYEQQEDFFRMMEIEKWITY
                     LILSFILLIAVFNIIGSLSMLMIDKKEDIKILENMGASQKLIFRIFLLEGWLISFVGM
                     IF"
     gene            complement(1005..1343)
                     /gene="rbfA"
                     /locus_tag="PK641_06680"
     CDS             complement(1005..1343)
                     /gene="rbfA"
                     /locus_tag="PK641_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HPG56063.1"
                     /translation="MEATRQNKIARLIQKDISEIFLGMTHTMPGTLVTTTNVRVSPDL
                     SVARIYLSIFPTERSQELLDLINSKSASIRYDLSQRTRFQLRTVPTLSFFIDDSLDYI
                     EHIDQLLKKL"
     gene            complement(1408..2040)
                     /locus_tag="PK641_06685"
     CDS             complement(1408..2040)
                     /locus_tag="PK641_06685"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPG56064.1"
                     /translation="MTEEIEQYIKQHSQKEDPLLTRLNRDANVKLLNPRMVSGHIQGR
                     LLSMISYMIRPKRILEIGTYVGYSAMCLAEGLAEGGKVITIDIDEEIEDMARAYIEQS
                     EYVDKIDFRIADALKLIPELDEVFDLVFIDGDKREYKAYYEASYDKLHQGGFMLIDNT
                     LWNGKVLNPAASWDHQTQALIEFNDFLAADERVDRFILDLRDGLTFVRKR"
     gene            complement(2037..>2316)
                     /locus_tag="PK641_06690"
     CDS             complement(2037..>2316)
                     /locus_tag="PK641_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPG56065.1"
                     /translation="NLVHKEIENLKSKKLSASQLQKLKAQWIAQMIIAGENRENWLMG
                     VVKQFMNTGKIESKKEIESIVSAISENELQTVAEEVFDEGKFTYLYYR"
BASE COUNT          747 a          486 c          306 g          777 t
ORIGIN      
        1 aaaaatcata cctacaaatg aaataagcca tccctcaagt aagaatattc taaaaataag
       61 tttttgtgat gcccccatat tttcaagtat cttgatatcc tcctttttat ctatcatcag
      121 catcgacaat gaacctatta tattaaacac agctatcaac aatataaaag acaaaataag
      181 atatgtaatc cacttttcaa tttccatcat tctaaaaaag tcttcttgtt gctcatatct
      241 attttgcaca gaaaaatcat cacctaacat tttagatata cttgcttgta cttttttttc
      301 atttgcacca tccttcaatt tgatttcgac ggcagatact tccttttcgt aatcaaataa
      361 acggcgagcc agtgacattg aaactatcaa caagttattg tcatattttt cttgttgcac
      421 cataaaaata cccgttaaaa aagcaatttc tcttttaaaa gcttcatccg gccttatcat
      481 attgacattc ccgcttcgtt taggcgcata taattgtaaa ggctctacat aatgcacacc
      541 acaacctata gttcgaacta agccaattcc acccactgcc atatcaaatg agccttcttt
      601 tgttttaaaa tcaccatcta atataatgtc ggatatgtga ttaattttag cataatcatc
      661 aggaacacct tttactacaa ccggagtttg cttatcacga aacactaaaa gcgcattttc
      721 ttgtaacacc tctctacaat attcaacatc tggcatttca gatatttttg acacaaaaac
      781 agggtcaaaa accttaactt gttttgcagt tatcttaagt tgaggatcaa aattgccgaa
      841 taagccttca acaattcctt gaaaaccatt aaatacagat aatacacata ccaaagccat
      901 tgtccccacc gcaacaccac acacagatat aagtgatact atattaattg cgtgatgcga
      961 tttttttgag aacaaataac gtccggctat gtatagcggc agattcacag tttctttagc
     1021 aattggtcta tgtgttcaat gtaatcaaga gagtcatcta taaagaatga caatgttggt
     1081 accgtacgta gttgaaaacg agtacgttgt gataaatcgt aacgaatgct tgcagattta
     1141 ctattaatta aatccaacaa ttcttgcgat ctttcagtag gaaatatact taaatatatt
     1201 ctagcaacgc ttaaatcggg actaacacga acatttgtag tagtaaccaa tgttccgggc
     1261 atcgtatgtg tcatgcccaa gaaaatttcg ctgatatctt tttgtatcag tcttgcaatt
     1321 ttattttgtc ttgttgcttc cataattttt tacaaaggta ttagttttca attaaaaaac
     1381 aagatttctt atgctaggca ttatttgtta acgcttacgc acaaaagtca aaccatcacg
     1441 cagatctaat ataaaacgat caacacgctc gtcagctgcc aaaaaatcgt tgaattcgat
     1501 aagagcttga gtttgatgat cccaagaagc agcaggattt aaaactttac cattccacaa
     1561 ggtattatca atcaacataa atccaccctg atgtaattta tcgtaagatg cttcataata
     1621 ggctttgtat tcgcgtttat ccccatcaat aaagaccaaa tcaaatacct catccaattc
     1681 gggtatcaac tttaaggcat ctgcaattct aaaatctatt ttatctacat attcagactg
     1741 ctcaatataa gcccttgcca tgtcttcaat ctcctcatct atatcaatag taattacttt
     1801 accaccttcg gccaaaccct cagccaaaca cattgcagag tatcctacat aagttccaat
     1861 ttcaagtatc cttttaggac gtatcatata cgatatcata cttaacaatc gaccttgtat
     1921 atgacctgat accatacgcg gattaagtag ctttacattt gcatctctat ttaaacgcgt
     1981 taataatggg tcttcttttt gcgagtgctg tttaatatat tgttcaattt cttcggtcat
     2041 ctatagtaca aataagtaaa tttaccttca tcaaaaacct cttctgcaac agtttgtaat
     2101 tcgttttcac tgatggcaga aacgattgat tcgatttcct tcttcgattc aatctttccg
     2161 gtattcataa attgtttcac tacacccatc aaccaattct ctctattctc acctgctata
     2221 atcatttgag ctatccattg agccttaagc ttttggagtt gactagctga taatttttta
     2281 cttttaagat tttcgatttc cttatgtacc aagttc
//
LOCUS       DAOPID010000228         2271 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_67279_length_2271_cov_4.779783, whole genome shotgun sequence.
ACCESSION   DAOPID010000228 DAOPID010000000
VERSION     DAOPID010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67279_length_2271_cov_4.779783"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="PK641_06695"
     CDS             complement(<1..787)
                     /locus_tag="PK641_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPG56066.1"
                     /translation="MEAEQIIRCLIIGSGPAGLTAAIYASRAGLKPVLYEGMLPGGQL
                     TMTTEIENFPGYPEGITGNEMMEDLHKQAARFGADIRFGVVTEVDFSQKPYKVVIEEK
                     TIYAHTVIIATGASAKYLGLADEHKYAGSGVSACATCDGFFYRKKTVAVVGGGDTACE
                     EALYLASLCKKVYLIVRRNVLRASLVMQERVLNKENIEVLFECQTLGLTGNGVVEGAD
                     LIKAKGSDTEEKFHIEIDGFFLAIGHQPNSKVFQKYITTDDVGY"
     gene            complement(805..1464)
                     /locus_tag="PK641_06700"
     CDS             complement(805..1464)
                     /locus_tag="PK641_06700"
                     /inference="COORDINATES: protein motif:HMM:NF027898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HPG56067.1"
                     /translation="MKLRLTIITWTISSLFCFVATAEGLVNQQSVSILDKVSETYKNS
                     SGTELDIKISMNDEKTKSSNSTTGNLKTKGNKFILTSDFATLIFDGKTLYVHSKITEE
                     ITISEPAKEDISGVDPTAILGIYKQGFKVNAPQEKSEKDKKTYLIELYPESHSAEYFK
                     IEIKIEADTYKPISIATYAKNGITNTILINKIQTNKEFTDKDFAFDSRKFPKAQIIDI
                     R"
     gene            complement(1466..2032)
                     /locus_tag="PK641_06705"
     CDS             complement(1466..2032)
                     /locus_tag="PK641_06705"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HPG56068.1"
                     /translation="MKIQWKPGTLIYPLPALMVSCGETETEYNIFTVSWTGTICSDPP
                     MCYISVRKSRHSHEIISRTKSFVLNLTTKQLAFATDWCGVRSGKDYNKFAEMKLTAEK
                     AQRVKAPIICESPLNIECEVTEIKEFGSHDMFIAKVVNIQADEKFIDPETGAFNMEEA
                     ELIAYSHGMYYKMGSRIGKFGWSVQKKK"
     gene            complement(2029..>2271)
                     /locus_tag="PK641_06710"
     CDS             complement(2029..>2271)
                     /locus_tag="PK641_06710"
                     /inference="COORDINATES: protein motif:HMM:NF014768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HPG56069.1"
                     /translation="IALIAPTAKINIIKNYEVVEKIDLALPNEVTEILQCVNPMCITN
                     KEKGITTRFNIIDKAKGTVKCHYCERIINQEDLILK"
BASE COUNT          618 a          405 c          375 g          873 t
ORIGIN      
        1 tatagccaac atcatccgta gttatgtatt tttgaaatac ttttgaatta ggttgatgtc
       61 ctatggctaa aaagaatccg tcgatttcta tatgaaattt ctcttcagtg tcactgcctt
      121 tagcttttat aaggtcagct ccttcaacaa cgccattacc cgttaaacct aatgtttgac
      181 attcaaacaa aacttctata ttctctttgt ttagtactcg ttcttgcatc actaatgatg
      241 ctctaagtac gtttctacga acaataagat atactttttt acataatgaa gctaagtata
      301 aggcttcttc acatgcagta tcacctccac ctacaacagc aactgttttt ttgcgataga
      361 agaatccatc gcaagtagca caagccgaaa caccggaacc tgcgtattta tgttcatcag
      421 ctaatccaag atattttgca gatgcacctg ttgcaataat aactgtatgt gcgtaaatag
      481 tcttttcttc tattacaact ttgtatggtt tttgtgagaa gtcaacttca gttacaactc
      541 caaatctgat atcagcacca aaacgtgcag cttgtttgtg caaatcttcc atcatttcgt
      601 tgcccgttat gccttcgggg tatcccggaa aattctctat ttctgtagtc attgttagtt
      661 gacctccggg cagcatacct tcgtatagaa caggcttaag acctgcgcga gatgcgtaaa
      721 ttgcagctgt taatccggca ggaccggaac caataattaa acaacgaatg atttgttcgg
      781 cttccatgat gttgattctt ttatttatct aatatcaatt atttgtgctt tgggaaattt
      841 tcttgaatca aatgcaaaat ccttatctgt aaactctttg tttgtttgaa tcttgtttat
      901 aaggattgta tttgttatgc cattttttgc ataagtggct atgctaatgg gtttgtaggt
      961 gtctgcttca atctttattt caattttaaa gtattcagca ctatgacttt caggatataa
     1021 ttcgattagg tatgtttttt tgtctttttc gcttttttct tgtggagcat ttactttaaa
     1081 tccttgcttg tagataccta aaatagctgt tggatcaact cctgaaatat cttcttttgc
     1141 cggctcacta atggtaattt cttcagttat tttagaatgt acatataaag tttttccatc
     1201 aaatattagt gtggcaaaat ctgaagttag tataaactta ttgcctttgg tttttaaatt
     1261 tccggtagtg gaattacttg atttagtttt ttcatcattc atagatattt taatatccaa
     1321 ttcagttcca cttgaattct tgtaagtttc agaaactttg tctaatatac taacagattg
     1381 ttgattaaca agaccttctg ctgttgcaac aaagcaaaaa agacttgaaa tagtccatgt
     1441 gatgattgtc aaacgtaatt tcatattatt tttttttttg tacagaccaa ccgaatttac
     1501 cgattctgga tcccatttta taatacattc catgtgagta agctatcagt tcagcttctt
     1561 ccatgttaaa tgcacctgtt tcagggtcta tgaatttttc gtcagcttga atatttacaa
     1621 cttttgcaat aaacatatcg tgtgaaccga attcttttat ttcggttact tcacattcaa
     1681 tgttaagtgg cgattcgcat ataataggag cttttactct ttgagctttt tcggctgtca
     1741 gtttcatttc tgcgaatttg ttgtaatctt ttcctgatct aactccacac caatcagttg
     1801 caaaagctag ttgttttgta gttaaattca atacaaaact tttggttcgt gagatgattt
     1861 cgtgtgagtg tcttgattta cgaactgata tataacacat tggtgggtca ctacaaattg
     1921 tgccggtcca actaactgta aatatattgt attcggtttc tgtttcgcca cagctaacca
     1981 ttaatgcggg gagtgggtat attaaagtac ccggtttcca ttgtattttc atttaagaat
     2041 aaggtcttct tggtttataa tccgttcgca atagtggcat tttacagtgc cttttgcttt
     2101 gtcaataata ttaaaacgtg ttgttattcc tttttcttta tttgtaatgc acataggatt
     2161 aacacattgt agtatttctg tgacttcatt cggtagcgct aaatcaattt tttctacaac
     2221 ctcgtagttc ttaattatgt tgatttttgc agttggtgca attaatgcaa t
//
LOCUS       DAOPID010000229         2246 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_68311_length_2246_cov_3.591511, whole genome shotgun sequence.
ACCESSION   DAOPID010000229 DAOPID010000000
VERSION     DAOPID010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68311_length_2246_cov_3.591511"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="PK641_06715"
     CDS             complement(<1..529)
                     /locus_tag="PK641_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPG56070.1"
                     /translation="MTTQDIIKPENLVYAKPSIMNDNTMHYCPGCSHGVVHKLIAEVI
                     EEMGIVDKAIGISPVGCAVFAYNYIDIDWQEAAHGRAPAIATAIKRLLPDRCVFTYQG
                     DGDLAAIGTAETIHACNRGENIAVIFINNAIYGMTGGQMAPTTLEGMKTSTCPYGRDV
                     TLNGYNLPITNLIAQL"
     gene            complement(537..1619)
                     /locus_tag="PK641_06720"
     CDS             complement(537..1619)
                     /locus_tag="PK641_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HPG56071.1"
                     /translation="MSEEVRLMKGNEAIAEAAIRCGCDGYFGYPITPQSEILETLMLL
                     KPWETTGMVVLQAESEVSSINMVYGGAGCGKKVMTSSSSPGFSLMQEGMSYLAGANVP
                     ALVVNVMRGGPGLGTIQPSQADYFQTVKGGGHGDYRQITLAPSSVQEMADFTVLGFEL
                     AFKYNIVCMILSDGLIGQMMEKVVLPPYTPRRTEEEIRKQCPWASLGKPANRKPNVIT
                     SLELDPARMEAINVSLQEKYRKIEENEVRFEETEMKDAEYLFMAFGSSARICQKAVEL
                     ARAEGLKVGLLRPITLFPFPTKQIKNQLSHIKGILSVELNAGQMVEDIRLAVEGKVPV
                     EHFGRTGGIVPTPEEVVEALKKQIVK"
     gene            complement(1621..1848)
                     /locus_tag="PK641_06725"
     CDS             complement(1621..1848)
                     /locus_tag="PK641_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPG56072.1"
                     /translation="MAKIKGAVLVNTDRCKGCELCITACPTNVLAMSKQVNAKGYNYA
                     YMENEDACIGCASCGIVCPDGCITVYKIKID"
     gene            complement(1916..>2246)
                     /locus_tag="PK641_06730"
     CDS             complement(1916..>2246)
                     /locus_tag="PK641_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG56073.1"
                     /translation="FSLWMLRIGVLLFLISKYTYVFKSFDYKNINYIIDAVTILSGIL
                     LFFGGFSKKSTLTIISGLCLCLLSLYQLYPLFINDKSITILLNTNVYTSFTIAATALL
                     FAAKGNK"
BASE COUNT          718 a          451 c          362 g          715 t
ORIGIN      
        1 ctaattgtgc aataagattt gtaataggta aattataacc atttaatgtt acatcacgac
       61 cataaggaca tgtagatgtt ttcatacctt caagggtagt tggtgccatc tgtccaccgg
      121 tcattccata tattgcgttg tttataaaga tgactgcaat attttcgcca cgattacaag
      181 catggattgt ttctgctgta ccaattgcag ctaaatcacc atcaccttga tatgtaaata
      241 cgcaacgatc aggaagtaaa cgtttaattg ctgttgcaat tgcaggtgca cgtccgtgtg
      301 cagcttcttg ccaatcaata tcgatgtaat tatatgcaaa aacagcgcat cctacaggtg
      361 aaattcctat agctttgtca acaatgccca tttcttcaat tacttctgca ataagcttat
      421 gaactacacc atggctacaa cccgggcagt agtgcatagt attgtcattc ataatcgaag
      481 gtttggcata taccaagttt tcaggtttta taatatcttg agttgtcatg attctgttat
      541 tttacaatct gttttttcaa tgcttcaact acttcttcag gagtgggaac tataccacct
      601 gttctaccaa aatgttcaac aggaacttta ccctctacag ctaaacgaat gtcttcaacc
      661 atttgacctg catttaattc aacagataaa atacctttaa tatgagatag ttgattttta
      721 atctgcttag tagggaatgg gaataaagtt attggacgta ataatccgac tttcaaacct
      781 tcagcacgag ctaattcaac agctttttga catatacgag cagatgaacc aaatgccata
      841 aacaaatatt cggcatcttt catttcagtt tcctcgaaac gaacttcgtt ctcttcaatt
      901 ttacgatact tctcttgcaa cgatacgttt atagcttcca tacgtgcagg atctaattcc
      961 aaagacgtga taacgttagg tttgcgatta gcaggtttac caagtgatgc ccaagggcat
     1021 tgttttctta tttcttcctc tgttctacga ggagtatatg gaggtaaaac aactttttcc
     1081 atcatctgac cgatcaagcc gtctgatagt atcatacata ctatattgta tttgaatgcc
     1141 aattcaaatc caagtactgt aaaatcagcc atttcttgta cagacgaagg tgccaatgta
     1201 atctgacgat aatcaccatg tccaccgcct tttactgttt gaaaatagtc tgcttggctt
     1261 ggttgaatag tacctaatcc aggacctcca cgcataacat taacaactaa tgccggaaca
     1321 ttagctcctg ctaaatagga cataccttct tgcatcaaac taaaaccggg gctggatgaa
     1381 gatgtcatta cttttttacc acatccggca ccgccataaa ccatatttat tgatgatact
     1441 tcactttctg cctgtaaaac taccattcct gttgtttccc acggcttaag tagcatcaaa
     1501 gtttctaaaa tttctgactg aggagtaata ggatagccaa aataaccatc acaaccacaa
     1561 cgaattgcag cctcggctat ggcttcatta cccttcatta gtcttacttc ttcactcata
     1621 ttaatcaatt tttattttat atactgtaat gcacccatcc gggcatacaa taccacaact
     1681 cgcacaacca atgcaggcat cttcattttc catgtatgca tagttgtacc ctttagcgtt
     1741 tacttgcttc gacatagcca atacatttgt tggacaagca gtaatgcata attcgcaacc
     1801 cttgcaacga tcggtattaa ccagtacagc gcctttaatt tttgccataa taagctattt
     1861 taaacaagct gcaaatttag taataatagt tgtaaaaaag aaacttaaaa agttattatt
     1921 tgttgccttt agcagcaaat aacaaagctg tagcagctat agtaaaagat gtgtaaacat
     1981 tagtgtttag taaaatagta atggatttat cgtttataaa cagaggatat aattggtaaa
     2041 gtgataataa acacaagcat aagcctgata ttatagtaag tgtcgatttt ttagaaaatc
     2101 ctccgaaaaa caataaaata cctgataata ttgttactgc gtcaataatg tagttaatat
     2161 ttttgtagtc aaaagactta aatacgtaag tgtattttga aattaagaac aacagaacgc
     2221 caatacgtag catccacaaa gaaaat
//
LOCUS       DAOPID010000230         2242 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_68481_length_2242_cov_4.039323, whole genome shotgun sequence.
ACCESSION   DAOPID010000230 DAOPID010000000
VERSION     DAOPID010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68481_length_2242_cov_4.039323"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PK641_06735"
     CDS             <1..328
                     /locus_tag="PK641_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPG56074.1"
                     /translation="KYLEDARLLNLIYAQGESFPKKPTCVYLNNSNLLYAIYGADVSA
                     QNISKTFVFSTLHGKCKLSKDAKKTNILVNGIYRLGLKDADTIICIDKSGRNTEIPIW
                     LFGMQY"
     gene            complement(332..1324)
                     /locus_tag="PK641_06740"
     CDS             complement(332..1324)
                     /locus_tag="PK641_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008822898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HPG56075.1"
                     /translation="MSKYLFMMFLLMLVGCTQQQNSYRKSVGRIFGTYYAVTYQSDED
                     LQAGIEQVLNDINSTFSTFDSTSIVSKINRNVNVKLNDDFVTVYNTAIRVSKQTKGAF
                     DITVAPLVNLWGFGYDPKRSRTQGEIDSIMLYVGYDKVSLRDSIIVKENPLTCLDASA
                     IAKGYACDAVAEYLKKNNINNFLIDIGGELTVMGKNPKGDNWVVGISKAIDDSTQTNS
                     ELERTLFITNKSIATSGNYRQYYITKERKVSHTIDPRTGYPTNHKLLSATVLASSCMI
                     ADAYATSMMVIGDTIEIDKIVKSSPDTLEWFLIVADSANVHHEVYSEGFNQYLR"
     gene            1477..1896
                     /locus_tag="PK641_06745"
     CDS             1477..1896
                     /locus_tag="PK641_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HPG56076.1"
                     /translation="MTKQNLLDKRYMRMAYVWAENSYCTRRQVGALLVKDKMIISDGY
                     NGTPCGFENICEDENTGKTKPYVLHAEANAITKVARSHNSSEGATMYVTTSPCIECAK
                     LMIQSGIKRVVYAEDYHITDGVELLKRAHIEVCKINL"
     gene            1901..>2242
                     /locus_tag="PK641_06750"
     CDS             1901..>2242
                     /locus_tag="PK641_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HPG56077.1"
                     /translation="MNNKRILYPIIIALVLCLGMIIGSYMSISTQKNDTESFDNTQSS
                     YDKLNAILNLLDQSYVDNIDRNKLTEDVLPEILSNLDPHSTYIPASEVEEANEDLEGS
                     FSGIGVQFNIQN"
BASE COUNT          780 a          390 c          355 g          717 t
ORIGIN      
        1 taaatattta gaggatgcac gtctgttgaa tttaatttat gcacaaggtg aatcgttccc
       61 taaaaaacca acttgcgtat atttaaataa ttctaactta ctatatgcca tatatggagc
      121 tgatgtatct gcacaaaata tcagtaaaac atttgttttt agcacactac atgggaaatg
      181 caaattaagc aaagacgcga agaaaaccaa catattggtt aatggcattt accgactagg
      241 tttaaaggat gcggacacca ttatatgcat tgacaaatca ggacgtaata ccgaaattcc
      301 tatatggtta tttggaatgc aatactgata tttaacgcaa gtattgatta aacccttcgc
      361 tataaacctc gtgatgcaca tttgcagaat cagcgacaat caaaaaccat tctaaagtat
      421 ccggtgatga ttttacaatc ttatcaattt caattgtatc acctataacc atcatacttg
      481 ttgcataagc atctgcaatc atacaacttg aagctaacac tgttgcacta agtaatttat
      541 gattagtcgg atatccggtt cgcgggtcaa tagtgtgtga aaccttgcgt tctttggtta
      601 tataatattg cctataattt cctgaagtag caatactttt gtttgtaata aataatgttc
      661 tttccaattc gctgtttgtt tgagtagaat cgtctattgc tttacttata ccaactaccc
      721 aattatcgcc ttttggattt ttacccatta cagtcaactc accacctata tcaatcaaaa
      781 agttattaat gttatttttc ttcagatact ctgccactgc atcgcaagca taaccttttg
      841 ctattgcact tgcatctaaa catgttaatg gattttcttt tacaattatt gaatctcgaa
      901 ggcttacttt atcataacct acatataaca ttatgctatc tatttcgcct tgtgtacgac
      961 tacgtttagg atcataacca aagccccata aattaaccaa aggggctaca gtaatatcaa
     1021 aagcaccttt tgtctgtttg gatacacgta tcgcagtatt atataccgtc acaaaatcgt
     1081 cattaagttt tacattaacg ttcctattta ttttagacac aattgaagta ctatcgaatg
     1141 tagagaaagt agaattaata tcgttaagaa cttgttcaat acctgcctgt aaatcctcat
     1201 cagactgata tgttactgca taataggtac caaatattct acccacagat tttcgatagc
     1261 tattttgttg ttgcgtacag cctactaaca tcaacaaaaa catcataaat aggtacttac
     1321 tcataaaatt gattttgatg tgcaaagata caatcattta ccaatttgta gtatatttgt
     1381 actcaattta ggattaaaaa ttgaatacaa aggactttta gagggattat taaaaatcta
     1441 cttaaagtat atatatactc taattttact ataatcatga caaaacaaaa tctacttgac
     1501 aaaagatata tgcgaatggc gtatgtatgg gcagaaaact catattgcac acgacgccaa
     1561 gtaggtgcac tattagtaaa agacaagatg ataatttctg atggttacaa tggtactccg
     1621 tgcggttttg agaatatttg tgaagacgaa aatacaggta aaacaaaacc atacgttttg
     1681 cacgctgaag caaatgctat tacaaaagta gcaagatcgc acaatagtag cgaaggagca
     1741 acaatgtatg ttacgacttc gccttgtata gaatgtgcca aacttatgat tcaatcaggc
     1801 ataaaacgtg tagtttacgc tgaagactat cacattacag atggtgttga attattaaag
     1861 cgtgctcata ttgaagtatg caaaataaat ttataataat atgaataata aaaggattct
     1921 atatccgata ataattgctt tagtactttg cctcggaatg attatcgggt cgtacatgtc
     1981 aataagtaca caaaagaacg atacagaatc atttgataat acgcaatcat catatgataa
     2041 actgaatgcc atattaaatt tattagacca atcttatgtt gataatattg acaggaacaa
     2101 gttaactgag gatgttttac ctgaaatact cagcaattta gacccccact ctacatacat
     2161 tcctgcaagc gaagttgaag aagccaacga ggatttggaa ggtagtttta gcggtatagg
     2221 tgtacaattc aatattcaaa ac
//
LOCUS       DAOPID010000231         2221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_69410_length_2221_cov_3.289012, whole genome shotgun sequence.
ACCESSION   DAOPID010000231 DAOPID010000000
VERSION     DAOPID010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69410_length_2221_cov_3.289012"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="PK641_06755"
     CDS             complement(<1..487)
                     /locus_tag="PK641_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPG56078.1"
                     /translation="MENIEYTAKDYKIGQPRWCPGCGDHAFLASLHKAMAELGVPPHE
                     IAVISGIGCSSRLPYYMNTYGMHTIHGRAAAIATGVKIANQNLTIWQISGDGDGLAIG
                     GNHFIHAVRRNIDLNMVLLNNKIYGLTKGQYSPTSERGFVSKSSPYGTIEDPFHPAEL
                     CF"
     gene            complement(490..>2221)
                     /locus_tag="PK641_06760"
     CDS             complement(490..>2221)
                     /locus_tag="PK641_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007567193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HPG56079.1"
                     /translation="GISTFPDYPADIRAPQGTLTGVSGFQVHIGTNKVLTPGDKCDVL
                     VAMNPAALKTQYKFCTPSTVIIIDSDSFKENDLKKALFTTLDPFEELGIKNEVVSAPI
                     AEICKEALKDSGLDFKSIIRCKNMFALGLVCWLFDRKLSLAEDMIRMKFAKKQEIADA
                     NIKVLNAGYDYGHNTHASTTTRYRIESTGIKEKGLYMDIDGNKATSYGFIAAAEKAGL
                     KLYLGSYPITPATDVLHELSKHKSLGVVTVQAEDEIAGCASAVGAAFAGALAVTSTSG
                     PGVCLKSEAMNLAVMTELPLIVLNVQRGGPSTGMPTKSEQTDLLQALYGRNGESPMPV
                     IAAASPTDCFDAAFSAAKIALEHMTPVVLLTDGFIANGSAAWKLPDISKYPSIKPPYV
                     TSDMEGKWTPFKRNEETGVRYWALPGTKGFMHRIGGLEKSNETGVISTEPENHDLMTR
                     LRQAKVDKIANSIPLQEVEGDPDADLLVVGFGGTYGHLSDAVGQMNKNGKKVALAHFK
                     YINPLPKNTETLLRKYKRIVVAEQNLGQLAAYLRIKIEGIHFYQFNQVKGQPFVTSEL
                     VSAFTKIMEE"
BASE COUNT          673 a          461 c          354 g          733 t
ORIGIN      
        1 caaaacaaag ctcagccgga tgaaaaggat cttcaatagt tccataaggt gatgatttac
       61 ttacaaaacc tctttctgaa gttggtgaat attgaccttt agttaaacca taaattttat
      121 tattcaatag aaccatattt aagtcaatat ttctacgaac agcgtgaata aaatggttac
      181 cgccaatagc caatccatct ccatcaccgg atatttgcca tatagttaaa ttctgatttg
      241 caatttttac accggttgca attgctgccg cacgcccgtg tattgtatgc ataccatagg
      301 tattcatata ataaggcaaa cgagaagaac aaccaatacc agatataaca gctatttcgt
      361 gaggtggtac acctaattcc gccatagctt tatgcaatga cgccaaaaat gcatggtcac
      421 cgcatccggg acaccaacgt ggttgtccta tcttataatc ttttgctgta tattctatat
      481 tttccatgat tattcctcca taattttagt aaatgcagat acaagttctg acgtaacaaa
      541 tggctgtcct tttacctgat taaactgata aaaatgtata ccttcaattt ttatacgaag
      601 atatgctgcc aattgaccta aattttgctc tgctactact attcttttgt atttacgtaa
      661 caaagtttct gtatttttag gtagtggatt tatatattta aagtgtgcta atgcaacctt
      721 cttaccattc ttgttcattt gacctacagc atcagaaaga tgtccgtatg ttccaccgaa
      781 accaacaact agcaaatctg catcaggatc accttcaact tcttgcaaag gaatactatt
      841 agcaatctta tcaacttttg cttgacgtaa acgcgtcatc aaatcgtgat tttcaggttc
      901 tgttgaaatt acacctgttt cattgctttt ctctaaacca ccaattctat gcataaatcc
      961 ttttgttcct ggtaatgccc aataacgcac gcctgtttct tcattacgtt tgaatggagt
     1021 ccatttgcct tccatatcag aagtaacata aggtggtttt attgaaggat attttgatat
     1081 atcaggtaat ttccatgctg ctgatccatt ggctataaac ccatctgtta ataaaacaac
     1141 aggtgtcatg tgctctaaag caatttttgc cgctgaaaat gctgcatcaa aacaatcagt
     1201 aggtgaagct gctgcaatta caggcattgg actttcacca tttctaccat acaaggcttg
     1261 cagtaagtct gtttgttctg acttagttgg cataccggtt gaaggaccac cacgttgcac
     1321 atttaatact attaatggca actctgtcat aacagcaagg ttcattgcct cactttttaa
     1381 gcaaactccg ggacctgacg tcgatgttac agctaaggca cctgcaaaag cagcacccac
     1441 tgctgatgca caaccggcaa tttcatcctc tgcttgcact gtaacaacac ccagagattt
     1501 gtgttttgac agttcatgta gaacgtcggt tgcaggggtt attggatatg agcctaaata
     1561 caattttaag ccggcttttt cagcagctgc aataaaacca taagatgtag ctttattacc
     1621 atcgatatcc atgtataaac ctttttcttt tatacctgta ctttcaatac gataccttgt
     1681 tgttgtagat gcatgagtat tatgaccata atcataacct gcattcaaca cttttatatt
     1741 tgcatctgca atttcctgtt tctttgcaaa tttcatacgt atcatatctt ctgccaatga
     1801 taattttctg tcaaaaagcc agcaaactaa acctaaggca aacatattct tgcaacgaat
     1861 aattgatttg aaatccaaac ctgaatcttt caatgcttct ttacatattt ctgctattgg
     1921 agctgaaact acttcatttt tgatacctaa ctcttcaaaa ggatctaaag ttgtaaataa
     1981 tgctttcttt aaatcatttt ctttaaatga atcactatct ataataatta cagttgaagg
     2041 agtacaaaat ttatattgtg ttttaagagc tgcaggattc atagcaacca aaacatcaca
     2101 tttatcaccc ggagttaata ccttgtttgt tccgatatgt acttgaaagc ctgaaactcc
     2161 tgtcaaagtt ccctgcggag cacgaatatc tgccggataa tcagggaatg tagatattcc
     2221 g
//
LOCUS       DAOPID010000232         2177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_71297_length_2177_cov_3.574929, whole genome shotgun sequence.
ACCESSION   DAOPID010000232 DAOPID010000000
VERSION     DAOPID010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71297_length_2177_cov_3.574929"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PK641_06765"
     CDS             <1..380
                     /locus_tag="PK641_06765"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HPG56080.1"
                     /translation="RNMLFLTFAAVLAKTKSCRYLITGVCETDFSGYPDCRDVFVKSL
                     NVTLNLAMDYNFVIYTPLMWLDKSQTWELADSLGYLDYVINNTLTCYNGIVGDGCGHC
                     PACKLRSRGLHNYLESKNKISDK"
     gene            382..858
                     /gene="queF"
                     /locus_tag="PK641_06770"
     CDS             382..858
                     /gene="queF"
                     /locus_tag="PK641_06770"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HPG56081.1"
                     /translation="MDDKLSLLGNKHTVYPKEYAPEVLETFVNKHPENDYFVKFNCPE
                     FTSLCPITGQPDFASIYISYIPAEKMIESKSLKIYLFSFRNHGDFHENCVNTIMKDLI
                     KLMSPKYIEVWGKFTPRGGISIDPYCNYGIPNSEYEKMALYRLQNHDLYPEKIDNR"
     gene            complement(870..>2177)
                     /locus_tag="PK641_06775"
     CDS             complement(870..>2177)
                     /locus_tag="PK641_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HPG56082.1"
                     /translation="GFTGGIALTIFSTQMNDLFGLGLTNVPADFIDKWICYIQNFVKI
                     DLWSLAIGLGSLLIIIFVPKISKKIPGSLIAIIFATLFVWLLKEYTDIAGIHGITTIA
                     DLYDLPSGMPSPKLPSIELKDGQTIMNVIQELFPAAFTIAMLGAIESLLSAIVADGVI
                     GDKHNSNTELIGQGIANIASPFFGGIPATGAIARTMTNINNGGRTPVAGIIHALVLLF
                     VLLLFGNLVGLIPMPCLAAVLIMVSYNMSGWKTFVSLAKNPKSDFAVLITTFVLTVVF
                     DLTIAIEIGLLLAIVLFLKRTSESTEIKVFHNEIDPSNDTDINLNEEKLKIPDGVEVY
                     EIDGPYFFGIANKFDEVMLETNEKPNVRIIRMRKVPFIDSTGLHNLETLCLQAKKEGI
                     KIVLSGVNDNVRKTFQKAGFEKYLSEEYICSNINEALEKAAKM"
BASE COUNT          687 a          356 c          404 g          730 t
ORIGIN      
        1 gtcgaaatat gttatttttg acctttgctg cagtattggc aaagacaaag tcatgtagat
       61 atcttattac aggagtgtgc gaaacagatt ttagtggtta tcctgattgc agagatgttt
      121 ttgtaaagtc tcttaatgtt acattaaatc tggctatgga ttataatttt gtaatttata
      181 cacctctaat gtggttggat aaatctcaga catgggaatt ggcagactct ttaggttact
      241 tagattacgt tattaataat accttgacat gctataatgg tattgttggt gacggttgtg
      301 gtcattgtcc tgcatgtaaa ttgcgcagta gggggctaca taactattta gaatcaaaaa
      361 ataaaatttc tgataagtaa tatggatgat aaactatcgt tattagggaa taagcacaca
      421 gtgtatccaa aggaatatgc acctgaggtt ttggagactt ttgttaataa gcatcctgag
      481 aatgactatt ttgtaaaatt caattgccct gaatttacca gtttgtgtcc aattacggga
      541 caacctgatt ttgcatcaat atatattagc tatatacctg ctgaaaaaat gattgaaagt
      601 aaatcactca agatttatct tttcagtttt agaaaccacg gcgattttca tgaaaattgc
      661 gtgaatacca ttatgaagga tttgattaaa ttaatgtctc ccaaatatat tgaagtatgg
      721 ggtaagttta ctccacgcgg tggcattagt attgatcctt actgcaatta tggaattcct
      781 aatagcgaat atgaaaaaat ggctctgtat cgtttacaga accatgattt gtatcccgaa
      841 aaaatagata atagataatt atctactaat tacatttttg cagctttctc cagtgcttcg
      901 ttaatattag agcatatata ttcttccgat aggtactttt caaatccggc tttttgaaat
      961 gttttgcgaa cgttgtcgtt aactccggaa agaactatct ttataccttc ttttttagct
     1021 tgtaggcata gagtttccaa gttgtgtaaa ccggttgagt caataaatgg tactttacgc
     1081 attctgatga tgcgaacgtt tggtttttcg tttgtttcaa gcattacttc gtcaaactta
     1141 tttgcaattc caaagaagta agggccatcg atttcgtata cttcaactcc atcaggaatc
     1201 tttaattttt cttcgttcaa attgatatct gtgtcgttgc ttggatcaat ttcgttatgg
     1261 aacactttga tctcggttga ttcgctggta cgttttaaaa acagtacaat agctagtagt
     1321 aagcctattt caattgctat ggttaaatcg aatactacag ttagcacgaa tgttgtaatt
     1381 aaaactgcga agtctgattt aggattttta gcaagtgata cgaatgtttt ccaaccgctc
     1441 atgttgtaag aaaccattat aagtacagct gctaagcatg gcatcggaat aagtccgact
     1501 aaattaccaa acaaaagtag aacaaatagt agaaccaatg cgtgtatgat tccggcaaca
     1561 ggagtgcgac ctccattgtt tatgtttgtc atagtacgtg ctattgcacc tgtggcaggt
     1621 atacctccaa aaaatggtga tgctatattt gcaatacctt gtcctattaa ttctgtattg
     1681 gaattatgtt tgtcgccaat aacaccatca gcaactattg cagatagaag agattcaata
     1741 gcgcctaaca tagcaatagt aaatgctgca gggaataatt cctgaataac attcataatg
     1801 gtttgaccat cttttaattc gattgaaggt aatttcggtg aaggcatccc cgaaggtaaa
     1861 tcatataaat cagcaatagt agtaatgcca tgtattcctg caatatctgt gtattccttt
     1921 agtaaccaca caaataaggt ggcaaaaata atagcaatta atgagcccgg tatctttttt
     1981 gagattttag gaacaaatat aattattaga agactgccta aaccgattgc caaagaccat
     2041 aagtctattt ttacaaaatt ttggatgtag caaatccact tatcaataaa gtctgcaggt
     2101 acatttgtca ggcctagtcc aaacaaatcg ttcatttgtg tagagaatat ggttagcgca
     2161 ataccaccgg taaaacc
//
LOCUS       DAOPID010000233         2151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_72542_length_2151_cov_3.263359, whole genome shotgun sequence.
ACCESSION   DAOPID010000233 DAOPID010000000
VERSION     DAOPID010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72542_length_2151_cov_3.263359"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1387
                     /locus_tag="PK641_06780"
     CDS             <1..1387
                     /locus_tag="PK641_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPG56083.1"
                     /translation="GWCKKNRKPDGSPYNIYTDGLRIYTTIDSRMQQYAEDAVREHVT
                     ETLQPAFFKEKKKKSYAPFSRLVSEEEIKGIMERSKRQSERYLKLKQANVPAAQINKI
                     FNTPVQMRVFDGKNDVDTIMTPLDSIRWDKYFLRCGFMSMDPISGHVKAYVGGPNFSE
                     FQYDMVTQGKRQVGSTIKPFLYTLAMEEGSQPCDKVPNIQPTFYLPSGDVWQPRNSLK
                     KRIGEMVTLRWGLANSNNWISAYLIKEYSPQSLVNLMHSFGILSQLDPVVSLCLGPAE
                     VSVKEMVGAYTTFVNRGIRVEPVYVTNIRDNKGNIITSFSPRTTEIINEKTSYEMLIM
                     LQSVINEGTGVRLRYRYGFRGQIGGKTGTTQNNSDGWFIGITPQLVNGAWVGGEDRSI
                     HFDSMSEGQGASMALPIWALYMKKVYADKTLGYSEDASFNIPSEISQLYDCKSQVESS
                     DDSDNIFGTDF"
     gene            1418..>2151
                     /locus_tag="PK641_06785"
     CDS             1418..>2151
                     /locus_tag="PK641_06785"
                     /inference="COORDINATES: protein motif:HMM:NF012889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding module family 20
                     domain-containing protein"
                     /protein_id="HPG56084.1"
                     /translation="MKLHFKISYFTHWGQNMAIIGSLPQLGENDSKKAALMNFQWRED
                     WTYDLEIKSSTPLEFKYKYVLKDVNGIDSHEWGDFRTVKINPNEVNDLYLVDTWNAPS
                     AIENVFMTSPFQDVLFRDNYIAVEAKETKKYTQIFKVKAPMLKHGEALCMVGDAKALG
                     SWDTTKALLMSNTESPWWTAKVDLSKETKNSVSYKFAVYDLEDKCFKYFENGPDRYAE
                     IKHEPKTQSIISASFANFDNKQFHGAG"
BASE COUNT          721 a          393 c          414 g          623 t
ORIGIN      
        1 tggttggtgt aagaaaaatc gcaaacccga tggctcaccc tataacattt atacagacgg
       61 cttacgtatt tacactacta ttgattcgcg tatgcaacaa tatgccgaag atgctgttcg
      121 tgagcatgta acagaaactc ttcaacctgc attttttaaa gaaaagaaaa agaagtctta
      181 tgctcctttc tctcgtttag tatcagaaga agaaatcaag ggcattatgg aacgttcaaa
      241 acgtcaatcg gaacgttatc tcaaacttaa acaggcaaat gtacctgctg cccaaataaa
      301 taaaattttc aacactcctg ttcaaatgcg agtgtttgac ggcaaaaacg atgtagatac
      361 cattatgaca ccgctcgact caattcgttg ggataagtat tttttacgtt gcggttttat
      421 gtctatggat cctataagcg ggcatgttaa ggcatacgtc ggaggtccta attttagcga
      481 attccaatac gatatggtta cccaagggaa acgtcaagta ggctcaacaa taaaaccatt
      541 cttgtacacc cttgcaatgg aagaaggctc gcagccatgc gataaggttc ctaatataca
      601 acctacattc tacttgccaa gtggtgatgt gtggcaacct cgtaattctt tgaaaaaacg
      661 tattggcgag atggtaactc ttcgatgggg attggcaaat tcaaacaact ggatatctgc
      721 ctatcttatt aaagaataca gccctcaatc attagttaat ttgatgcatt cattcggtat
      781 cttaagtcaa ttggatcctg tagtttctct ttgcttaggt cctgccgaag tgtctgttaa
      841 agagatggtt ggagcatata ctacctttgt aaacagaggt atccgtgtag agcctgttta
      901 tgttaccaat attagagata ataaaggaaa cataataaca agtttctcgc ctcgtactac
      961 cgaaataata aatgaaaaaa cctcatacga gatgcttata atgctgcaat cggttattaa
     1021 cgaaggtacc ggtgttcgct tacgttatcg ttacggcttt aggggtcaaa taggaggtaa
     1081 aaccggaaca acacagaata attctgatgg ttggtttatc ggcatcactc ctcaattagt
     1141 caacggagca tgggtaggag gtgaagatcg ttcaattcac ttcgatagta tgtccgaagg
     1201 tcaaggagca tcaatggcat taccgatatg ggccttgtat atgaaaaaag tttatgcaga
     1261 taaaacatta ggatattcag aggatgcctc atttaacata ccaagtgaaa tatcacagct
     1321 ttacgattgt aaatctcaag ttgagagttc tgatgattct gataatatat tcggtaccga
     1381 tttttaaaac aatataaacc ctttaaacaa aatacttatg aaactccatt ttaaaatcag
     1441 ctattttacg cactgggggc aaaatatggc aatcattggc tcattacctc aattaggaga
     1501 aaatgattca aaaaaagcag cactaatgaa ttttcaatgg cgcgaagatt ggacttacga
     1561 cttagaaatt aaaagttcca caccattaga atttaaatac aaatatgttc ttaaagatgt
     1621 aaatggtatc gacagccacg agtggggcga tttcagaacc gttaagatta atcctaatga
     1681 agttaacgat ttatatttgg ttgatacttg gaatgcacct tctgctatcg aaaacgtgtt
     1741 tatgacatct cctttccaag atgtactatt tcgtgataat tatatagctg tagaagctaa
     1801 agaaacgaaa aaatatacac aaatcttcaa ggtaaaagct cctatgctta aacacggcga
     1861 agccctttgt atggttggtg atgccaaagc cttaggctca tgggacacta ctaaagcact
     1921 gcttatgtct aatactgagt ctccatggtg gaccgcaaaa gttgatttaa gtaaagaaac
     1981 aaaaaattca gtaagctata aatttgcagt atacgacctt gaggataaat gctttaagta
     2041 ttttgaaaac ggtcccgaca gatatgccga aataaaacat gaacctaaaa cacaatctat
     2101 aatttccgca tcctttgcaa attttgataa taaacaattc catggtgcag g
//
LOCUS       DAOPID010000234         2145 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_72832_length_2145_cov_3.365072, whole genome shotgun sequence.
ACCESSION   DAOPID010000234 DAOPID010000000
VERSION     DAOPID010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72832_length_2145_cov_3.365072"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="PK641_06790"
     CDS             <1..481
                     /locus_tag="PK641_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HPG56085.1"
                     /translation="VELLKQFKNKYPNLQLILEPGSAFAWRTGVLVSTVQDIVENHGI
                     KTAMLDVSFACHMPDCLEMPYKPSIVGATDAVNEKPTYRMGGNSCLSGDYIGDWSFDK
                     ELSIGDKIVFEDMIHYTIVKTTMFNGVSHPSIGMLTADNKFILLKTFAYDDYKNRMS"
     gene            816..888
                     /locus_tag="PK641_06795"
     tRNA            816..888
                     /locus_tag="PK641_06795"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:849..851,aa:Gly,seq:gcc)
     gene            990..1073
                     /locus_tag="PK641_06800"
     tRNA            990..1073
                     /locus_tag="PK641_06800"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1024..1026,aa:Leu,seq:cag)
     gene            1082..1165
                     /locus_tag="PK641_06805"
     tRNA            1082..1165
                     /locus_tag="PK641_06805"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1116..1118,aa:Leu,seq:gag)
     gene            1181..1975
                     /locus_tag="PK641_06810"
     CDS             1181..1975
                     /locus_tag="PK641_06810"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPG56086.1"
                     /translation="MKFCSFASGSDGNCYYIGTDTCGILIDAGISIKTIKKNLKQLGI
                     SLSSIYAVFVTHDHYDHIASVGTLGEVYNIPIYSTQAILEGINRCYKVSDKLYQSKHV
                     QELGTTINIANISITSFHVNHDSTQCVGYYVESEGDNLVIATDLGYINDEAIPFLLKS
                     NNLVIETNYDEEMLLQGSYPLFLKKRILGNNGHLANHVTARFLAENHQDHWKHIFFCH
                     LSKENNSADAVLSMLSKQYDTCGIKSEERPELTILPRTTPIDLCII"
BASE COUNT          710 a          330 c          402 g          703 t
ORIGIN      
        1 agttgaatta cttaagcaat ttaaaaacaa gtatcctaat ttacaattga tactagaacc
       61 cggtagtgct ttcgcatggc gtaccggagt attagtatct acagttcaag atattgtaga
      121 aaatcatggc attaaaacag caatgttaga tgtgtctttt gcctgccaca tgcccgattg
      181 tttggaaatg ccatataaac cttctatagt tggcgctaca gatgctgtta atgaaaaacc
      241 tacatatcga atggggggta atagctgctt gtcgggcgat tatattggcg actggtcttt
      301 tgataaagag ctttctattg gcgataaaat agtgtttgag gatatgatac attatacaat
      361 agttaaaacc actatgttta acggagtttc gcatccatca ataggtatgt tgactgcaga
      421 caataaattt atattgctaa aaacatttgc ttatgatgat tataaaaatc gcatgtcata
      481 atatcgctta tttcattctt tacttttcaa tatatccggc tagttaaaca ttattagata
      541 aaggattcca aatttttatt tgattctaaa ataacgatat atgcacactt gcattgcatg
      601 tataattttt ataactacat cattagcaga tgctttggta aaagcatgga agccaccatc
      661 ttagccaatt gattagcatt cttgccatat ttaagttttc tcatttgaag gtcaaatttg
      721 agtctggcat tactttttta ttatttttct tgcataatat aaaaaatgcg tttatctttg
      781 caccgcaatt gacgaagagg ggtaatgatc ataatgcgaa aatagctcag ttggtagagc
      841 acgaccttgc caaggtcggg gtcgcgggtt cgagtcccgt ttttcgctct cggaaaaaat
      901 aaggttaggg ttgtaaaatt ttacagagaa cctaaaatcc aattgaaata gaaagaggcg
      961 tccgttttgg atgcctcttt tggttagatg cccaaatggc ggaattggta gacgcgctag
     1021 tttcaggtac tagtaatcga aaggtcgtgc aggttcgagt cctgttttgg gcacccttta
     1081 cgcataggtg gtgaaattgg tatacacgct actttgaggg ggtagtaccg gcaacggtgt
     1141 gtgagttcga gtctcatcct atgcacaaaa aaaatctata atgaaatttt gtagttttgc
     1201 cagcggaagt gatggtaatt gttattatat aggcacagat acatgcggca ttttaattga
     1261 tgcaggcatc agtataaaga caattaaaaa aaatctaaaa caattaggca tatcgctttc
     1321 ttcaatatat gctgtatttg ttactcatga tcattacgat catatagctt ctgtcgggac
     1381 cttaggcgaa gtctataata ttcctatata ttctactcaa gcgatattag agggcattaa
     1441 tcgttgttac aaggtaagtg ataagttgta tcagagtaag catgtgcaag aattaggtac
     1501 aacaataaat atagcaaata tttcaattac atcttttcat gttaatcatg attcgaccca
     1561 atgtgtagga tattatgttg aatcagaagg cgataacctt gttattgcta cagatttggg
     1621 atacattaac gatgaagcaa ttccattttt gctgaaatca aataatttag ttatagaaac
     1681 aaattatgat gaagaaatgc tattgcaagg ttcttatcca ttgtttctga aaaaacgaat
     1741 attgggcaat aatggacatt tggcaaatca tgttactgca cgttttttag cagaaaatca
     1801 tcaagatcat tggaaacata ttttcttttg ccatctaagt aaagaaaaca attcagctga
     1861 tgcagtatta agtatgttgt caaagcaata cgacacttgc ggaataaaat cagaagaacg
     1921 tcccgaatta actatattgc ctcgtactac tccaatagat ttatgcataa tataacatca
     1981 aaaatgcata aaaatgcaat aaaattataa tttattccta aatattgaat aaattttcat
     2041 tttcttttgt aaagaatgat ttttttacta cttttgtgtc gatttaaaag aaaagaagat
     2101 gagtgaaaac acgaaaatca aaacattaga ccaggttgta gttcg
//
LOCUS       DAOPID010000235         2132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_73407_length_2132_cov_4.269138, whole genome shotgun sequence.
ACCESSION   DAOPID010000235 DAOPID010000000
VERSION     DAOPID010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73407_length_2132_cov_4.269138"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            218..523
                     /locus_tag="PK641_06815"
     CDS             218..523
                     /locus_tag="PK641_06815"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPG56087.1"
                     /translation="MKKLLFVILLSSASIFAFAENLSLDVVAGQQSVSVSFEQSESQI
                     VTIYIYNMLGKVIVAEKINAVSGKNNLELYTTDMVKGAYIAKIITSKGETKAIRFNY"
     gene            complement(675..>2132)
                     /locus_tag="PK641_06820"
     CDS             complement(675..>2132)
                     /locus_tag="PK641_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPG56088.1"
                     /translation="NKCAYVCPHACIRPFVMNEEESAGLNAEMIEMKAPATMKGMNFR
                     IQVSVMDCLGCGNCADVCPGNPKAGGPALKMVPLEQELKEAANWEYCINNVKSKQDLV
                     DIASNVKNSQFATPLFEFSGACSGCGETPYLKLISQLYGSRQMVANATGCSSIYSGSV
                     PSTPYTTNEKGQGTAWSNSLFEDFCEYGMGMELANEKMRARLTKLMKDAQSCTCCSDE
                     LKALFNEWVENQNDAAKTQELYDKIMPQVKACNCDTCKKIEALSGYIVKRSQWIIGGD
                     GASYDIGYGGLDHVIASGKNVNILVLDTEVYSNTGGQASKSTPVGAIAKFAASGKRVR
                     KKDLGMIATTYGYVYVAQIAMGADQAQCLKAIREAEAYDGPSLIIAYSPCINHGLKNG
                     MGKAQEEEAKAVACGYWHLWRYNPSLEAEGKNPFILDSKEPNWEGFKDFLKSEVRYSS
                     VMKQYPLEAEQLFEAAEDNAKWRYKSYQRMLNQQF"
BASE COUNT          647 a          391 c          368 g          726 t
ORIGIN      
        1 tcttgattta gataaactgc ggcaaatgta caaattattt actgcataat aagaattaaa
       61 gtgtgagtgg gtaagtcaga attgattata aaaataacaa cattttcttg ttatcgtttg
      121 cgtaaattga atttaaatgt gaattttatt aagaaaatgc ttaaataatt tggaaatcat
      181 atcatttttt tttaattttg tggcgtaacc gtgaagtatg aaaaaattat tatttgtcat
      241 attattatct tctgcttcaa ttttcgcttt tgccgaaaat ttgagtttag atgttgtagc
      301 cggtcaacag tctgtatctg ttagttttga acagtcagaa tctcagattg taacgattta
      361 catatacaat atgttaggga aggtgattgt agcagaaaag attaatgctg ttagcggtaa
      421 aaataatctg gaactttata ctacagacat ggtaaaaggt gcttacatag caaaaattat
      481 tacttcaaaa ggcgaaacaa aagctattag atttaattac taatacattt caaatactta
      541 taattaagag tctctttatc gaggctcttt tttttgtctt gttatataca aaaaagagtc
      601 gcagtttaaa actgcgactc tttttgattc atgtcttaat gtttaagcga taagacatat
      661 aagttaaggt tctattagaa ctgttgattc aacatacgtt gatagctctt ataacgccat
      721 tttgcattat cttcggcagc ttcaaacaat tgttctgctt ctaaaggata ttgtttcata
      781 actgaagagt agcgaacttc actttttaag aagtctttaa atccttccca attaggttct
      841 ttgctatcta agatgaatgg gttctttcct tctgcttcca aagatgggtt ataacgccac
      901 aagtgccaat atccgcaagc aacagctttt gcttcttcct cttgtgcttt acccatacca
      961 tttttcaagc catggtttat acaaggagag taagcaataa ttaatgaagg accatcgtaa
     1021 gcttctgctt cgcgaatagc tttcaagcat tgtgcttggt ctgcgcccat tgctatttgt
     1081 gcaacatata cgtatccgta tgtagttgca atcataccaa ggtctttctt acgtacacgt
     1141 ttacctgaag cagcaaattt tgcaattgca ccaacaggag tagattttga tgcttgacca
     1201 ccggtatttg agtaaacctc agtatctaat accaagatat ttacattctt accggatgcg
     1261 attacgtggt ctaagccacc ataacctata tcgtaagaag caccatcacc accaataatc
     1321 cattgtgaac gtttaacgat gtatccgctc agagcttcta ttttcttgca tgtatcacaa
     1381 ttgcaagcct taacttgagg cataatcttg tcatacaatt cttgtgtttt agcagcatca
     1441 ttttgattct caacccattc gttgaataat gcttttaatt cgtcagaaca gcaagtgcaa
     1501 gattgggcat ctttcatcaa ttttgttaga cgtgcacgca ttttttcgtt tgccaattcc
     1561 atacccatac cgtattcgca gaaatcttca aataaagaat ttgaccatgc ggtaccttgt
     1621 cctttttcat ttgttgtata aggagtagaa ggaactgagc ctgaataaat tgaagagcaa
     1681 ccagttgcat ttgcaaccat ttgacgacta ccatataatt gagagatcaa ttttaaataa
     1741 ggtgtttcgc cgcaacctga gcaagctcct gaaaactcga atagtggagt tgcaaattgt
     1801 gagtttttga cattagatgc aatatcaacc aaatcttgtt tagattttac gttgttaatg
     1861 caatattccc aatttgctgc ttcttttaat tcttgttcaa gtggaaccat ctttaatgca
     1921 ggaccaccgg cttttggatt acccggacaa acgtcagcgc agttaccgca accaaggcag
     1981 tccataacgc taacttgaat acggaaattc attcccttca ttgtagcagg tgctttcatt
     2041 tcaatcattt cggcatttaa gcctgcagat tcctcttcgt tcattacaaa tggacggata
     2101 caagcgtgag gacaaacata agcacatttg tt
//
LOCUS       DAOPID010000236         2117 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_74136_length_2117_cov_4.326382, whole genome shotgun sequence.
ACCESSION   DAOPID010000236 DAOPID010000000
VERSION     DAOPID010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74136_length_2117_cov_4.326382"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="PK641_06825"
     CDS             complement(<1..757)
                     /locus_tag="PK641_06825"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HPG56089.1"
                     /translation="MLNTPYQLIRVCAAVPKTIVADTIANADAICEAVCKADDECSHI
                     VVFPELCVSGYTCADLFFQNSLIEACKDAISHIIQATAHKKMCIIIGSPLIIDNNLYN
                     CAIVINQGQIIGIVPKSLLPNYNEFYEKRWFCPADMLSSDSIKLCGQNVPVGTDLIFN
                     INDNFKFGIEICEDLWSPIPPSSFLCLAGADIIFNLSASNELVSKRAYRQRLIRQQSA
                     RAICGYVYCNAGNGESTSDIVFSGHCMIAENGRI"
     gene            complement(761..>2117)
                     /locus_tag="PK641_06830"
     CDS             complement(761..>2117)
                     /locus_tag="PK641_06830"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPG56090.1"
                     /translation="SLSANKSVDGTFDSPYENIAKKRKFSHIDVVAQTDNGNTEYVYD
                     GDKSAILIYKNKDGKWRKSGKLKFWKNRVRKIGFNSNCEQIVFSSEGKKSLGEQDLFI
                     VNKNGKKWTKPENLGNQVNTEYDETAPVFVNDSTIYFSSTGHNSMGGYDIFKTTLSNG
                     KWGQAENIGTPINTAFDDVFYTPTNKDLKAYISSNRDGGKGGMDIYALTYILPKELAE
                     DFDAPAGTIGDKLLAGINIQQEANIDSSKVTLMKGIIMGDDSLLLKAKIELADNELQQ
                     IIATFEADSTGNYEVILLGGLNYGISVSYPGYLFHSENFDLPEQSEFQEIDKDIILKK
                     IAIGKNIVLKNIFFETAKADLNDESIIELNNVIKLLKENPTMHVEISGHTDNVGSKAY
                     NKNLSNFRAKSVVDYLIQNGIEEGRLTSKGYGYDNPVASNKTEEGRKENRRTEFLVTK
                     I"
BASE COUNT          639 a          387 c          334 g          757 t
ORIGIN      
        1 aaatacgacc attttctgca atcatgcaat gaccgctaaa aactatatca ctagttgatt
       61 ctccattgcc ggcattacaa taaacatatc cgcaaatagc tcttgccgat tgttgtctga
      121 ttaagcgttg tctgtacgca cgttttgaaa ctagttcgtt gctggcagat aaattaaaga
      181 tgatgtcagc acctgccaaa cacaaaaatg aactaggagg gataggtgac cataaatctt
      241 cgcatatttc aataccgaat ttaaaattgt cgtttatatt gaagattaaa tcagttccga
      301 caggaacatt ttgtccgcac aatttaatag agtcagagct aagcatatca gcaggacaaa
      361 accaacgttt ctcgtaaaat tcgttgtagt ttggcagtaa acttttgggt acaatgccga
      421 ttatttgtcc ttgattaatt acaatagcac aattgtatag attgttatca ataattagag
      481 gactgccaat aataatacac atttttttat gcgcagttgc ctgtattata tgcgatattg
      541 catctttaca agcttcaatc aaagagtttt gaaagaataa atccgcacaa gtatatccgg
      601 aaacacacaa ttcaggaaat acgactatat gagagcattc gtcatctgct ttgcaaacag
      661 cttcgcaaat ggcatctgca ttggcaatag tatcagctac tattgttttt ggaacagctg
      721 cacaaactcg tattaattga tatggtgtat ttagcataat ttagattttt gtaacaagaa
      781 attctgtgcg gcgattttct ttacgacctt cttctgtttt gtttgatgct acaggattat
      841 catatccata tccctttgaa gttaatcgtc cttcttctat accattttga attaaataat
      901 ctactactga ttttgctcta aagttagata agtttttatt gtatgcttta gaacctacgt
      961 tatcggtgtg tcccgatatt tcaacatgca ttgtaggatt ctctttcaat agtttaataa
     1021 cattattcaa ctctatgata gattcatcat ttagatcagc tttggcagtt tcaaagaaaa
     1081 tattcttaag tacgatattt ttgcctatag caatcttttt aagaataata tctttgtcga
     1141 tctcttgaaa ttctgattgt tcaggtaaat caaagttttc agaatggaat aaatatcccg
     1201 gataactaac agagatacca taattcaagc ctccgagaag tataacttcg taatttccgg
     1261 tactatcagc ttcaaaagtt gcaatgattt gttgtaattc attatcagcc aattcaattt
     1321 ttgcttttag aagtaggcta tcatctccca taataatacc tttcatcaaa gttacttttg
     1381 aactatcaat attagcctct tgttgaatat taattccggc taataattta tctccaatag
     1441 tacctgcggg tgcatcaaaa tcttctgcaa gttcttttgg aaggatgtaa gtaagtgcat
     1501 atatatccat gccacctttg ccgccatctc ggtttgaaga tatgtatgcc tttaagtctt
     1561 tgttggtagg agtgtaaaac acatcatcaa aagctgtgtt tataggagtc ccaatatttt
     1621 ccgcttgtcc ccatttccca ttacttaatg ttgttttaaa tatatcatat ccacccatag
     1681 agttatgacc ggttgacgaa aaatatatgg tcgagtcatt tacaaataca ggagctgttt
     1741 cgtcatattc agtgttaacc tgattaccta aattctccgg ttttgtccac tttttaccat
     1801 ttttatttac tataaaaaga tcttgttcac ctaaactctt tttgccttcg ctagaaaata
     1861 ctatttgctc acaatttgag ttaaatccaa tttttctgac acgatttttc caaaatttaa
     1921 gtttgcctga ttttctccat ttaccgtcct tgtttttgta aataaggata gctgatttat
     1981 ctccgtcata aacatattct gtattgccgt tatcggtttg tgcaacaaca tcaatatgag
     2041 aaaattttct tttttttgcg atattctcgt atggagagtc aaatgttcca tcaacgcttt
     2101 tgtttgcaga caaagag
//
LOCUS       DAOPID010000237         2064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_76887_length_2064_cov_4.576406, whole genome shotgun sequence.
ACCESSION   DAOPID010000237 DAOPID010000000
VERSION     DAOPID010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76887_length_2064_cov_4.576406"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1879
                     /locus_tag="PK641_06835"
     CDS             <1..1879
                     /locus_tag="PK641_06835"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPG56091.1"
                     /translation="GRVIPVIEDEYVDIEFGTGCLKVTPAHDVNDYMLGEKYNLPTID
                     IFNDNGTISEAAGIYVGMDRFDVRKQIEKDLEAADLLEKTEAYTNKVGYSERTRVVIE
                     PKLSMQWFLKMEHLAKPALDEVMNDNLKFYPPKYKNTYRHWMENIKDWCISRQLWWGH
                     RIPAYYLPQGGFVVAKSAEEALVIAKQKTGDEFLTIKDLKQDEDCLDTWFSSWLWPIS
                     LFDGINKPGNKEISYYYPTDDLVTGPDIIFFWVARMIMAGYEYKGDMPFKHVYFTGIV
                     RDKLGRKMSKSLGNSPDPLDLISKYGADGVRMGMMLAAPAGNDVHFDEALCEQGRNFN
                     NKIWNALRLVKGWQVSDSTQPEIAKMAVEWFDEQLSATIEEVNDQFDKYRLSEALLRV
                     YKLFWDEFSSWYLEMIKPAYGQAIDNVTYQATLKFFEKLCLLLHPFMPFITEEVWQVI
                     SERKNGESIMYASWPFSGKTNPKFLDSFERTKELVSAIRAVRLEKNIPFKDDLRLQVL
                     SNGHDNSYNTVVAKMCNISSIEEISEKEAMSVSFLVRTVEYAIPLAKSLNVEEEIKKT
                     DAEIKYLEGFLVSVEKKLSNEKFVANAKPEVVEIERKKKADAESKLSSMRLTLSQLSK
                     N"
BASE COUNT          717 a          301 c          411 g          635 t
ORIGIN      
        1 tggacgtgtt attcctgtaa ttgaagatga atatgttgat atagagtttg ggaccggttg
       61 cttaaaagta actccggcac atgatgttaa cgactatatg ttgggtgaaa aatataattt
      121 acctacaata gacatcttta atgataatgg tacaataagt gaagctgccg gtatttatgt
      181 aggtatggat aggtttgatg tacgcaagca aattgaaaaa gatttggaag ctgcagattt
      241 actcgaaaaa accgaagcct atactaataa agtcggatat tccgaacgta cccgtgtagt
      301 tattgaacct aaattgtcga tgcagtggtt cctaaaaatg gagcatcttg caaaacccgc
      361 tttagatgaa gtaatgaacg ataatcttaa attctatcct ccaaaataca aaaatacata
      421 tcgtcattgg atggagaata ttaaggattg gtgcatcagt cgtcaacttt ggtgggggca
      481 tcgaatacct gcttattatt tgcctcaagg tggttttgta gtagctaaat cggctgaaga
      541 ggctttggtt atagcaaaac agaaaacagg agatgagttc ttgaccatta aagacttaaa
      601 acaagacgaa gattgcttgg atacatggtt ttcatcatgg ttatggccta tatcactatt
      661 cgacggaata aacaaacccg gaaataaaga aatctcatat tactatccaa ctgacgactt
      721 ggttacagga cctgatatta tattcttttg ggtagcacgt atgataatgg caggctatga
      781 atataaaggt gatatgccat ttaaacatgt ttatttcaca ggtatcgtgc gtgataagtt
      841 aggtagaaag atgtcaaaat cattaggtaa ttcgcctgat ccacttgatt tgatttcgaa
      901 atacggagca gatggtgttc gtatgggtat gatgttggca gcccctgccg gcaatgatgt
      961 acattttgat gaggcattat gcgaacaagg acgtaatttt aataataaga tttggaatgc
     1021 actgcgtttg gttaaaggtt ggcaagtttc tgattctaca caacctgaaa tagcaaaaat
     1081 ggcagttgaa tggtttgatg aacaattgag cgcaactata gaggaagtaa atgatcaatt
     1141 cgataaatat cgtttatcgg aagcattact tcgtgtttat aaattgtttt gggatgagtt
     1201 ttcttcttgg tatcttgaaa tgattaaacc ggcttatggc caggctatcg acaatgttac
     1261 atatcaagca actttgaaat tctttgaaaa attatgtttg ctattgcatc catttatgcc
     1321 gtttattaca gaagaagtat ggcaagttat ttcagaacgt aaaaatggtg aaagtataat
     1381 gtatgcatct tggccatttt caggcaaaac aaatcctaaa tttttagatt cttttgaacg
     1441 taccaaagaa ttggtatcag ccatacgtgc agtccgttta gaaaaaaata ttccatttaa
     1501 agatgatttg cgtttacaag tattatctaa cggacatgat aattcttaca atacagttgt
     1561 cgctaaaatg tgtaatatat cttcaataga agagatatct gaaaaggaag ctatgtcagt
     1621 ttctttctta gtgcgtactg ttgaatatgc tattccatta gcaaaaagtc tgaatgttga
     1681 agaagaaata aaaaaaacag atgctgaaat caaatattta gaaggatttt tagtttctgt
     1741 tgaaaaaaaa ttaagtaacg aaaaatttgt tgctaatgca aaacctgaag tcgttgaaat
     1801 cgaacgcaag aaaaaggctg atgcagaaag caagttgtct agtatgcgtc ttacattgtc
     1861 gcaattatct aagaactaat ctaataatca acaaataatt attttaaaat cgttaacgca
     1921 atcatgagtg agcaaccttc aaaaatttca ttctacaaag gagataatat tcctttggag
     1981 atgcacaaag ttagaattgt acaaaaacta tttttaccaa aaatagaaga tcgtaaacaa
     2041 aacatgaatg atgccggcaa caat
//
LOCUS       DAOPID010000238         2032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_78637_length_2032_cov_3.790086, whole genome shotgun sequence.
ACCESSION   DAOPID010000238 DAOPID010000000
VERSION     DAOPID010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78637_length_2032_cov_3.790086"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="PK641_06840"
     CDS             complement(<1..1259)
                     /locus_tag="PK641_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HPG56092.1"
                     /translation="MKHPKGLYLLFFTEMWERFSYYGMRALLVLYLTKSFIDGGLGFS
                     DGSATMLYGIFTGLVYFSPLIGGWIADNYIGQRKSIIIGSIVMMLGQFTLASGQNTGC
                     LYIGLIFLIIGNGFFKPNISVLLGSLYPQGDSRRDSAFTIFYMGINLGAFLAPLLTGY
                     VAVVYDYRYGFLISGLGLLIGFVMYLLGGEKLLGDAGKLPSAKKNIDTDIDSKLTLEE
                     KDRTWVIVVLTLFAVAFFAGYEQAGSSMTLYADKYIDRVVFDKLVPTEWFQAVNPLFI
                     IILAPLLSMLWGFLSRRGKEPSVPVKMGYGLILLGLGFMILLMAIVQRGGDVSSTHVK
                     ANVWFLIGAYFMHTVGELCLSPIGLSMVTKLSPLKLSSLMMGVWLASSSIANLLAGFL
                     ATFASNAGASYVFIALAISSVFLGLVL"
     gene            complement(1269..>2032)
                     /locus_tag="PK641_06845"
     CDS             complement(1269..>2032)
                     /locus_tag="PK641_06845"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004346057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPG56093.1"
                     /translation="EHVNESIYNIIKNVFYKSETTLPYKADTGAEDGYLIGAEDGAEA
                     YENGLHFLIDWEKGQKTGFFIDQRENRALLEHYAKGRKVLNMFCYTGGFSCYAMRGEA
                     SAVYSVDSSAKAIALTEKNLINNFKDDSRHKEFALDAFKYLDDIKKDDYDLIILDPPA
                     FAKHRAALHNALQGYKRLNLKAFEKIKSGGILFTFSCSQVVTKEQFRLAVFSAAAMSG
                     RKVRILHQLTQPADHPVNIYHPEGEYLKGLVLYVE"
BASE COUNT          715 a          444 c          286 g          587 t
ORIGIN      
        1 attaagacta aacccaaaaa tactgaactt attgctaatg caataaagac gtatgatgct
       61 cctgcattcg aagcaaatgt tgctaaaaat cctgccaaaa ggttagctat tgaactactt
      121 gctaaccaaa cacccatcat taaagatgat aatttaagtg gagacaattt tgtaaccata
      181 gacaaaccta ttggtgacaa acacaattcg ccgactgtgt gcataaaata agcaccgatc
      241 agaaaccaaa catttgcttt tacatgcgtt gaactaacat ctccaccacg ctgaacaata
      301 gccattaaca aaatcataaa accaagtccc aaaagaatca agccatatcc catcttaacc
      361 ggtaccgatg gctcttttcc tcgtcttgat aaaaatcccc acaacataga tagcaaagga
      421 gctaatatta tgataaacaa cggattaact gcttgaaacc attctgtagg taccaattta
      481 tcaaaaacca cacgatcaat atatttgtcg gcatacaaag tcattgaact acctgcttgt
      541 tcataacctg caaagaatgc cactgcaaat aatgtcaaaa caacaataac ccaagttcta
      601 tctttctctt cgagtgtaag ttttgaatca atatcggtat caatattttt ctttgcagac
      661 ggaagtttac cggcatcacc aagcaatttt tcacctccaa gtaaatacat tacaaaacct
      721 attaacaagc ctaaacctga aatcaaaaaa ccatacctgt aatcatacac aactgcaaca
      781 taacctgtta aaagaggagc taaaaatgca ccaagattga tacccatgta aaatattgta
      841 aacgctgaat cacggcgcga atcgccttgt ggatacaaac tgcctaacaa tacagaaata
      901 ttaggtttaa agaagccatt accaattatt agaaagatca agccgatata caaacatcct
      961 gtattttgac cggaagccaa ggtaaattga cctagcatca ttactatcga ccctattatt
     1021 atggattttc tctgacctat gtaattatca gctatccatc cacctatcaa tggcgagaaa
     1081 tatactaaac cggtaaatat accgtatagc atcgttgctg agccatccga aaatcctaac
     1141 ccaccatcta taaagctttt agttaaatac agcactaata atgcacgcat gccataataa
     1201 ctaaaacgtt cccacatttc agtaaaaaac aatagatata atcctttggg atgcttcata
     1261 cagtttatct attctacgta caaaaccaga ccttttagat attctccttc cggatgataa
     1321 atattaacgg gatgatcagc cggttgagtc aactgatgta agattctaac ttttcgacca
     1381 ctcatggctg ctgcagaaaa aacagctaat cgaaattgtt ctttagtaac aacctgcgaa
     1441 cacgagaatg taaataggat tccaccggac ttaatcttct caaatgcctt taaatttaga
     1501 cgtttatatc cttgtaaagc attgtgcaag gctgctctgt gcttagcaaa tgcaggagga
     1561 tcaagaataa tcaaatcgta gtcatctttt ttaatatcat caagatactt aaatgcatca
     1621 agtgcaaatt ctttatgtcg actatcgtcc ttaaaattat ttataagatt cttttccgtt
     1681 aaagctatcg ccttagcaga actatcaacc gaataaactg ctgatgcctc accacgcatg
     1741 gcatagcaag aaaagcctcc tgtgtaacaa aacatattca acacctttct tccttttgca
     1801 taatgctcaa gcaatgccct gttttcacgc tgatcaataa aaaagcctgt tttttgacct
     1861 ttttcccaat ctattaaaaa gtgtaagcca ttctcataag cctctgcacc atcttcagca
     1921 ccaattaagt atccatcttc tgctcctgta tctgctttat atggtaaagt agtttctgat
     1981 ttatagaata catttttaat aatattataa atagattcat tgacatgttc tt
//
LOCUS       DAOPID010000239         2018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_79503_length_2018_cov_3.447784, whole genome shotgun sequence.
ACCESSION   DAOPID010000239 DAOPID010000000
VERSION     DAOPID010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79503_length_2018_cov_3.447784"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..678)
                     /gene="purE"
                     /locus_tag="PK641_06850"
     CDS             complement(163..678)
                     /gene="purE"
                     /locus_tag="PK641_06850"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HPG56094.1"
                     /translation="MEKLQAQVSIIMGSTSDLAIMEKAAKVLDEFQIPFEMNALSAHR
                     TPQAVEEFAKNAEKRGVKVIIAAAGMAAHLGGVIASMTTLPVIGVPIKSSLEGMDALL
                     AIVQMPPGIPVATVGINGSMNAGILAVQIIALQDERVNKQLKEYKENLKKKIEKANEE
                     LKDIKFAYKTN"
     gene            complement(680..1753)
                     /locus_tag="PK641_06855"
     CDS             complement(680..1753)
                     /locus_tag="PK641_06855"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPG56095.1"
                     /translation="MRTILHILFLIISVISFGALIITDVIAYIQPSSVLSVFSLLSVA
                     FPFLFITSFCLLLFWIIQKRWIYATALFLLLLISTPNLLKVYSFKPSRIEQSSEDAYS
                     ISSFNVHYFSFFEKKDGKRLGISILDSISKSKVDIICIQEMGYENTYIYTLEYIKRKL
                     KDYPYMQIRNVDNNSHFQKAIATFSKYPIIAKHNIEFDSRYHCAIASDILINNDTLRV
                     INCYLESNRLTEQEKEIYHSDDKENLIKRIYKKLGNASVKRNAQAAKIASFSKQSKLK
                     TIICGDINDVPTSYVYRTIRGDYEDTFLSLCTGMGNTFHEKIYRFRIDYIFTDKRITP
                     LSFSIGKQDDSDHYPISMSFKLN"
     gene            complement(1750..>2018)
                     /locus_tag="PK641_06860"
     CDS             complement(1750..>2018)
                     /locus_tag="PK641_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG56096.1"
                     /translation="YALLLKKHNIDISFQITQIINFFSTFSFTKPKMKVSTNSNYKTD
                     EQWNMERKSKNSRIDEILDKIKKSGYEQLTEDEKKELFDLSNKK"
BASE COUNT          611 a          309 c          328 g          770 t
ORIGIN      
        1 aaatatattg tagagtttcc gaaaataatg aggcatcatg tttgatgctg gaaaaatcgg
       61 gatttgtgag aacttgtgtg cttaaagatt ggctcagaac agagtctggt tttgaaaacg
      121 tagttatgta ccaattgata gcacataact acgtagatta atttagttgg tcttataagc
      181 aaactttata tctttaagtt cttcgtttgc tttttcgatt ttctttttaa gattttcttt
      241 gtattccttt agttgcttgt ttacacgttc atcttgcaaa gcaatgattt gtacagctaa
      301 gatgccggca ttcattgatc cattgattcc tacagtagca acaggtattc ccggaggcat
      361 ttgtactata gccaatagtg catccatacc ttctaaactt gatttgatag gtactccgat
      421 aacaggtaat gttgtcatag atgcaataac tcctcccaaa tgagctgcca taccggcggc
      481 agcaataatt actttaacac ctcttttttc tgcatttttt gcaaattctt caacagcttg
      541 tggtgttcta tgagcagaca atgcgttcat ttcaaatggg atttgaaatt catccaatac
      601 ttttgcagct ttttccatta tagctaaatc gctggtgctt cccattatga tacttacttg
      661 ggcttgtagt ttttccatat tagtttaatt tgaatgacat agaaatcgga taatggtctg
      721 aatcatcttg tttgccaata ctaaaagaca atggcgtaat tcttttatct gtaaatatat
      781 agtcgattct gaacctgtaa attttttcgt ggaatgtatt tcccatacca gtgcataaag
      841 ataagaaagt atcttcataa tctccgcgta tggttctata aacatacgaa gtaggcacat
      901 catttatatc accgcagata atagtcttaa gctttgattg cttgctgaat gaggctattt
      961 ttgcggcttg tgcattcctt ttgacagatg cattacctag ttttttgtat attcttttaa
     1021 ttaaattttc tttatcatca gaatgatata tttctttttc ttgttccgtt aacctgttcg
     1081 attcgaggta gcaattaatg actcttaatg tatcattatt aataagtatg tctgacgcaa
     1141 tagcgcaatg atatctggaa tcaaattcaa tattgtgttt tgcaataata gggtatttcg
     1201 agaatgttgc tattgctttt tgaaagtggc tattattgtc aacatttctt atttgcatgt
     1261 aaggataatc ttttaatttt ctttttatat attcgagagt atatatatat gtattttcat
     1321 atcccatctc ttgaatacat ataatgtcaa cttttgactt tgatattgaa tctaatatac
     1381 ttattccaag tcttttccca tctttttttt caaaaaacga gaaataatga acattgaaac
     1441 tagatatgct gtaggcatct tcagaagatt gttcgatcct agaaggttta aatgaataaa
     1501 cctttaataa gtttggtgta gatataagta gtaagagaaa taatgctgta gcgtagatcc
     1561 atcgtttttg aataatccaa aataataata aacaaaagct tgtaataaat aagaaaggaa
     1621 aagctacgct caacaaacta aaaacagata aaacagatga gggttgtatg taagcaataa
     1681 cgtcagtaat gatcagtgcg ccaaatgaaa tcacgctgat tatgagaaat aatatatgta
     1741 atatggttct cattttttgt tgctcaaatc aaataattct tttttttcat cttctgttaa
     1801 ctgctcataa cctgattttt tgattttgtc taatatttca tcaatgcggc tattctttga
     1861 tttgcgttcc atattccatt gttcatcagt cttatagttg ctatttgtgc ttactttcat
     1921 tttaggtttt gtaaatgaaa atgtcgaaaa aaagtttatt atttgtgtta tctgaaaact
     1981 gatatcaata ttgtgctttt ttagtaataa tgcatata
//
LOCUS       DAOPID010000240         1949 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_83449_length_1949_cov_4.363252, whole genome shotgun sequence.
ACCESSION   DAOPID010000240 DAOPID010000000
VERSION     DAOPID010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83449_length_1949_cov_4.363252"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PK641_06865"
     CDS             <1..385
                     /locus_tag="PK641_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG56097.1"
                     /translation="KVRFDETIVAYGYEDALFSWALYQNGVPMYHINNPLENRLVSNK
                     CFLKQCSTAVRNLSYMVKNDNFPQNVRLVKIYNKIKSFHMANLCAYVSYAINPLIYAL
                     LYLNVPFLFLLDAYKLSLFCRYMKS"
     gene            493..1110
                     /gene="recR"
                     /locus_tag="PK641_06870"
     CDS             493..1110
                     /gene="recR"
                     /locus_tag="PK641_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007133351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HPG56098.1"
                     /translation="MSDIQSYPSFLLENAVNQFAKLPGIGRKTALRLALHMLKQEKND
                     VDDFAQSILLLKNEVKYCKICKNISDNDVCQICSDLTRDAGLVCVVETIKEVMLIEHT
                     GQYKGLYHVLGGVISPMDGIRPNDLEIDSLVKRVSEGGVREIVMALSTTMEGDTTAYY
                     ISKKLSEFDLTLTTIARGIAVGDELQYADELTLGRSIVNRVPYKN"
     gene            1115..1537
                     /locus_tag="PK641_06875"
     CDS             1115..1537
                     /locus_tag="PK641_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG56099.1"
                     /translation="MNKKQAQKQLFAIYYAIYGLLVLELVIAHLLPNNLSLSANTIMI
                     MQGVVICLLLALIPTALKLYSVKIKIKSDLSYYIHWSRIQMILLYIPGFASGVLYYFV
                     RDKSSLFCLLIAFVALIFSKPTPAKIDNYINEDEGIES"
     gene            1537..>1949
                     /locus_tag="PK641_06880"
     CDS             1537..>1949
                     /locus_tag="PK641_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG56100.1"
                     /translation="MTIAVDFDGTIVTHEYPKIGEEIPFAIETLKRLQSEIGCRLILW
                     SVRSGKLLDEAVEYCLSKGLEFYAINKDFPEEEYDEASNRKINADIYIDDRNVGGLPD
                     WGVIYAMVKGQTRPIYDTQYIKPKPKNLIIRFGEW"
BASE COUNT          669 a          282 c          362 g          636 t
ORIGIN      
        1 aaaagttcgt ttcgacgaaa caatagttgc ttatggttac gaagatgcat tgttttcatg
       61 ggcattatat caaaatggag tacctatgta ccacataaat aatccattgg aaaatagatt
      121 ggtatctaat aagtgtttct tgaagcaatg ctcaacagca gtaagaaatc tgtcatatat
      181 ggtgaaaaat gacaatttcc cacaaaacgt gcgcttggtt aaaatataca acaaaataaa
      241 atcctttcat atggcaaact tatgtgctta tgtaagttac gcaatcaacc cacttattta
      301 tgctttacta tatttgaatg ttcctttttt atttttgttg gatgcttata aattgagtct
      361 tttctgccga tacatgaaat cgtgatagta tttcttctta tgtgctactc ttagttaaaa
      421 gtactaatat tcaatcctat atctgttgtt agaaattata agaataacta tatttgtagc
      481 ttatttaata taatgtcaga tattcaatca tatccttctt ttttactgga gaatgctgta
      541 aatcagttcg ccaagttgcc cggaatcggt aggaagactg cactccgact agccttgcat
      601 atgttaaagc aagagaaaaa cgatgttgat gattttgcac aatcaatatt gttgctaaaa
      661 aacgaagtca aatattgcaa aatatgtaaa aatatctctg ataatgatgt ctgtcaaatt
      721 tgctcagatc taacacgtga tgcaggtttg gtttgtgtgg tcgaaacaat taaagaagtt
      781 atgttgattg aacacacagg tcaatacaaa ggactatatc atgtattagg tggagtaatc
      841 tctccgatgg atggaatacg cccaaatgat ttggaaatcg atagccttgt taaacgggtg
      901 tctgaaggcg gagttagaga aatagtaatg gctttaagta ccacaatgga aggtgacact
      961 actgcatatt atatctctaa aaaattgtct gaatttgatc tgacactaac aactatagca
     1021 cgtggaatag cagtgggtga tgaacttcaa tatgccgacg aattgacatt aggccgatct
     1081 atagtaaaca gagtacctta taaaaactaa ttatatgaat aaaaagcaag cccaaaaaca
     1141 attatttgca atatactatg caatttatgg tttactggtt ttagaattgg tgatagctca
     1201 tttactacca aataatctta gcttatctgc aaataccatt atgataatgc aaggagtagt
     1261 gatatgttta ttactagcct taatccctac agcattaaaa ttgtattcgg taaaaattaa
     1321 aattaaatct gatttgtcgt attatataca ttggtcaaga attcagatga ttttacttta
     1381 tatacccgga tttgcatcag gggttttgta ttattttgta agagacaaat caagtttatt
     1441 ttgccttttg atagcttttg ttgcattgat attcagcaag ccgacaccgg caaaaataga
     1501 taattatatt aacgaagatg aggggataga atcatgataa ctatagcggt tgattttgat
     1561 ggaaccattg taacgcatga gtatccaaaa ataggagaag agattccttt tgcaatagaa
     1621 actttaaaac gcttacaatc agaaatcgga tgtagattga tactttggtc tgtgcgctcc
     1681 ggtaaattat tagatgaggc tgttgaatat tgtctaagta aaggtttgga attttacgca
     1741 attaataaag attttcctga agaagaatat gatgaggcga gcaatagaaa aattaatgcc
     1801 gatatatata ttgatgatcg aaatgttgga ggattacccg attggggagt tatatacgcc
     1861 atggtaaaag gtcaaacgcg accgatttat gatactcaat acataaaacc gaaacctaaa
     1921 aatttaatta ttcgttttgg cgagtggtt
//
LOCUS       DAOPID010000241         1935 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_84258_length_1935_cov_4.789894, whole genome shotgun sequence.
ACCESSION   DAOPID010000241 DAOPID010000000
VERSION     DAOPID010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84258_length_1935_cov_4.789894"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PK641_06885"
     CDS             complement(<1..532)
                     /locus_tag="PK641_06885"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HPG56101.1"
                     /translation="MFTNKTKRINKKKSSNESSERPSIIGKTTLVIVTTIAAIFLVTL
                     LINAIIIGFPTLRISKTEFPSSYFIQNSNRIDIQTKHECAGYATAYAIRALGKEADGM
                     KIYEALKKTGSGDVYAKNICKYIEKEGFYANFHLGNIDQIQRDLSNGNIVIAIIKTYQ
                     GSLLTHYVPVVGYDEKN"
     gene            complement(505..>1935)
                     /gene="aspS"
                     /locus_tag="PK641_06890"
     CDS             complement(505..>1935)
                     /gene="aspS"
                     /locus_tag="PK641_06890"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPG56102.1"
                     /translation="FTIEDDTDGGDDLRMKYRYLDLRRSFVRKNLELRHKMTMEVRRY
                     LDSQGFLEVETPMLIGSTPEGARDFVVPSRMNPGQFYALPQSPQTLKQLLMVSGFDRY
                     FQIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVINLFESMTKHLFKTLRGIELKET
                     FPRMTWHDAMKYYGSDKPDLRFGMKFVELDMVLKGCGFGVFDNAAYIGGICAHNAAVY
                     TRKQLDNLTEFVKKPQIGANGLVYARVEADGSVKSSVDKFYTQEKLQELKVAMNANPG
                     DLILIMSGDNVNKTRKQLCELRLEMGNQLGLRDKNKFACLWVIDFPMFEWSDEENRLM
                     AMHHPFTHPKDEDIPLLDTNAAEVRADAYDMVVNGVEVGGGSIRIHDPKLQARMFEIL
                     GFTPEKAQEQFGFLMNAFKFGAPPHGGLAYGLDRWVSLFAGLDSIRDCIAFPKNNSGR
                     DVMLDAPAPIEQKQLDELCLQTKQKE"
BASE COUNT          543 a          388 c          332 g          672 t
ORIGIN      
        1 tgttcttttc gtcatatcct acaaccggaa cataatgcgt tagtaaagaa ccttgataag
       61 tcttgattat tgcgataaca atattaccat tactaagatc tcgttgtatt tggtcaatat
      121 tgccaagatg aaaatttgca taaaatcctt ctttttcaat gtatttgcaa atattttttg
      181 cgtaaacatc gccagaaccg gttttcttaa gtgcctcata tattttcatt ccatcagctt
      241 cttttcctaa tgctctaatg gcataagctg tagcgtatcc tgcacattca tgtttagtct
      301 gtatatcaat gcggttggaa ttttgtatga agtatgatga agggaattct gttttgctga
      361 ttctcagagt tggaaatcca attattattg cattaataag taatgttaca agaaaaatag
      421 cagcaatagt tgtgactatc acaagagtag tctttccaat tatggaggga cgttctgatg
      481 attcattgga gctctttttt ttgtttattc tttttgtttt gtttgtaaac ataattcgtc
      541 taattgcttt tgttcgattg gagccggtgc atctaacata acatcgcgtc cgctattgtt
      601 tttagggaaa gcgatacagt cgcgaatact gtctaagccg gcaaacaaac taacccaacg
      661 atccaaacca tatgctaaac ctccgtgagg tggtgctcca aatttgaatg cgttcattaa
      721 aaatccgaat tgttcttggg ctttttcagg tgtgaaacct aaaatctcaa acattcttgc
      781 ttgtaactta ggatcgtgga tacgtattga accaccacct acttcaactc cattaactac
      841 catatcgtat gcatcagccc taacttctgc agcattggta tcgagtaggg gaatatcttc
      901 gtcttttgga tgagtaaatg ggtgatgcat cgccataagt ctgttttctt catcgctcca
      961 ttcaaacatc gggaagtcaa taacccaaag acatgcaaat ttatttttat ctcttaagcc
     1021 taattgatta cccatttcta aacgcaattc gcataattgt ttccgagttt tattaacatt
     1081 atcgccactc attatcaata ttaaatcgcc tggatttgcg ttcattgcaa cttttagctc
     1141 ttgtaatttc tcttgtgtat agaatttatc tacacttgat tttacactac catcagcttc
     1201 aacacgtgcg tatactaaac cattagctcc tatttgcggt ttttttacaa attcggtaag
     1261 attatccaat tgttttcttg tgtaaacggc tgcgttatgt gcgcatatac cgccaatata
     1321 agcagcattg tcaaacacac cgaatccgca tccttttaaa accatatcta attctacaaa
     1381 cttcattcca aaacggaggt ccggtttgtc actcccatag tatttcatag catcatgcca
     1441 tgtcattctt ggaaatgttt ctttaagttc gatgccccgt aatgttttga ataaatgttt
     1501 tgtcattgac tcaaacaagt taattacgtc ttcttgctca acaaacgaca tttcgcagtc
     1561 tatttgtgta aattcaggtt gacggtctgc tcgtaagtct tcatcgcgaa agcattttac
     1621 tatttggaag tatctatcaa aaccacttac cattagtaat tgcttaagag tttgaggtga
     1681 ttggggtagt gcatagaatt gtccgggatt catacgactt ggtacaacaa agtcgcgtgc
     1741 tccttcggga gtagaaccta ttagcatagg agtttctact tctaaaaatc cttggctatc
     1801 aagataacgg cgaacttcca ttgtcatttt gtgccttaac tccaggtttt tgcggacaaa
     1861 actacgacgt aagtccaaat aacgatattt catgcgtaga tcatcgcctc catcggtatc
     1921 atcttcaatt gtaaa
//
LOCUS       DAOPID010000242         1920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_85237_length_1920_cov_4.854692, whole genome shotgun sequence.
ACCESSION   DAOPID010000242 DAOPID010000000
VERSION     DAOPID010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85237_length_1920_cov_4.854692"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1217
                     /locus_tag="PK641_06895"
     CDS             <1..1217
                     /locus_tag="PK641_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HPG56103.1"
                     /translation="TPDEQLPEAVAVLDPKRLDSTYPYGHLSGCGVGFKLMQAFAINN
                     HIPFERLTQHLDLLAVSIASDIVPITGENRVLMTFGLKQLNSNPSMGLQAILDICGLQ
                     GKEVTVSDIVFKIGPRINASGRMYSGREAVELLTSKDRDFAKEKSNCIDQYNQERKDL
                     DKKTTVDAQDFINGDEHFENRNSIVVYNPEWSKGVIGIVASRLSEQYYRPSVVLTLSN
                     GFATGSARSVSGFDIYKAIDSCRDLLENFGGHIYAAGLTMKEENVSEFSRRFDEYVTS
                     NMNEDQKIPQIEVDSIVHFSEITPKFFSILQKFAPFGPENMKPVFVTENVKDYGTSRL
                     VGRDLDHFKLDLTEDESPNVIMNGIAFGKGKEFNDHIKAKKSFNVCYTIEENNFAGKE
                     AIQLLIKDIKTD"
     gene            complement(1270..>1920)
                     /locus_tag="PK641_06900"
     CDS             complement(1270..>1920)
                     /locus_tag="PK641_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPG56104.1"
                     /translation="IRTEKVAIKDIKLRTFISEGNIRNELAAHVYDITYGSIVPHKDN
                     LVVIDDSIVRGTTLRQSIIRILARLEPKKIVIVSSSPQIRYPDFYGIDMSSLEDFIAF
                     RAAVALLHDNNSEYILDEVYEKSKQQEFFPKEKIVNYIKEIYAPFSAKQISAKIAELL
                     TPNEVNCHIDVIFQSLEGLHEACKNNVGDWYFSGDYPTPGGNKRLNQSYINYYENK"
BASE COUNT          648 a          325 c          344 g          603 t
ORIGIN      
        1 acactcctga tgaacaatta cctgaagcag ttgcagtatt agatcccaag cgtttggaca
       61 gtacataccc atacggacat ttatccggat gcggtgtagg ttttaagttg atgcaggcct
      121 ttgcaattaa caatcacatt ccttttgaac gtttaactca acatcttgat ttactggctg
      181 taagtatcgc atcagacata gttcccataa caggagaaaa tagagttttg atgactttcg
      241 gattaaagca actaaattca aatccgagca tggggctaca agctattctt gatatatgcg
      301 gacttcaagg taaggaagta actgttagtg atatagtatt taaaataggt cctcgtatta
      361 acgcatcagg aagaatgtat tcgggtcgtg aagctgttga acttctaact tcaaaagatc
      421 gcgattttgc aaaggaaaag agcaactgta ttgatcaata taatcaagaa agaaaagact
      481 tagataaaaa gactacggtt gatgctcaag actttataaa tggagatgaa cattttgaaa
      541 accgcaattc tatagtagtt tataaccctg aatggagtaa aggagtgata ggaattgttg
      601 catcaagatt gagcgaacaa tattatcgtc cttctgtggt actaactcta tcgaatggat
      661 tcgcaacagg ttcggcacgt tctgtaagcg gatttgatat ttacaaggct attgattctt
      721 gcagagattt attagaaaac tttggaggtc atatttatgc agcaggctta acaatgaaag
      781 aagaaaacgt tagtgagttt tcgcgcagat ttgatgaata tgtaacttca aacatgaacg
      841 aagaccaaaa gattccacaa attgaggttg attcaatagt acatttcagc gaaattactc
      901 ctaagttttt tagcatcctt caaaaatttg caccatttgg ccctgaaaat atgaaacctg
      961 tgtttgtaac tgaaaacgta aaagactatg gtacaagcag attggtaggg cgcgatttag
     1021 accacttcaa attagacttg acagaagacg aatcgccaaa cgtaattatg aatggcatag
     1081 cttttggtaa agggaaggaa tttaatgatc acataaaagc taaaaaatca tttaatgtct
     1141 gctacacaat agaagaaaac aactttgcag gaaaagaagc cattcaactt ctgattaaag
     1201 atattaaaac cgactaatca actgattata atatattatg attcatacat tctaataaga
     1261 gtgtgtgaat catttgtttt cataatagtt tatatacgat tgatttaaac gcttattacc
     1321 tccgggtgta ggataatctc ctgagaaata ccaatcaccg acattatttt tgcatgcctc
     1381 atgtaatcct tccaaactct gaaatataac atcaatgtga caattaacct cattaggagt
     1441 taataactcc gctatttttg cagaaatttg tttcgctgaa aatggagcgt aaatttcttt
     1501 tatataattc acgattttct cttttggaaa aaattcttgt tgttttgatt tctcgtaaac
     1561 ctcatctaag atatattcag aattattgtc atgcagcaat gcaacagcag ctctaaatgc
     1621 tatgaaatct tctaaagatg acatgtctat tccatagaaa tcaggatagc gtatttgagg
     1681 agatgaggac actataacaa tctttttagg ttctaatctt gctaatattc taataatact
     1741 ctgcctcaat gttgtgcctc taacaatgct atcatcaata accacaagat tgtctttatg
     1801 cgggactata ctaccataag taatatcata tacatgtgcc gctaattcgt tacggatatt
     1861 tccttccgat ataaacgttc gtaacttaat gtccttaatt gccacttttt ctgtgcgtat
//
LOCUS       DAOPID010000243         1887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_87419_length_1887_cov_3.402838, whole genome shotgun sequence.
ACCESSION   DAOPID010000243 DAOPID010000000
VERSION     DAOPID010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87419_length_1887_cov_3.402838"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1342
                     /locus_tag="PK641_06905"
     CDS             <1..1342
                     /locus_tag="PK641_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPG56105.1"
                     /translation="EKPTLIIGKTLMGKGAKGPDGQSFENKVSTHGQPLTNAGASIEN
                     TIVNLGGDPQNPFCIFPEVAALYEKRKNELAEIVAKRKAEAASWKKENPELSEKYTKF
                     YDKSFSKINFSEIQHKLDQSTRSASGSMLSLFSGKIENMIVASADLSNSDKTDGFLKN
                     SKPLAKGDFSGGFLNAGVSELTMACIMNGLALHGGVIPVCGTFFVFSDYMKPAFRMSA
                     LMRLQVIYVWSHDAFRVGEDGPTHQPIEQELQVRLMESLKNHKGKNSMLVLRPADESE
                     TTVAWQMALENTDSPTGLILSRQNIKSLPRPADRYADALNMKKGAYVVKNVEKPDVIL
                     LASGSEVATLFEAANLLEDKKNLKVKIVSVPSEGVYFTQSESYRSTVIDYNIPIFGLT
                     AGLPMTLQRIAGLKGRVFGLDHFGHSAPYNVLDEKFGFTAENIYNQVCDYLANY"
     gene            1342..>1887
                     /locus_tag="PK641_06910"
     CDS             1342..>1887
                     /locus_tag="PK641_06910"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HPG56106.1"
                     /translation="MDIITIVLIAIGLSMDCFAVSIAKAGTLNKFDFGKFFAMALLFG
                     AFQGFMPFIGYLLGYNFAAYIESVDHWLALLILSIIGGKMIFEDLKPKNINEDIAEKN
                     PYSIQTLLLLSVATSIDALATGLVFIPYKEWLWIAISVIAIVSFVFSFAGSMLGLFFG
                     KKFSFKFGIVGGIILIAIGVKI"
BASE COUNT          597 a          289 c          380 g          621 t
ORIGIN      
        1 agaaaaaccc acattaatta ttggcaaaac attaatgggg aaaggtgcaa aaggtcctga
       61 tggtcaaagc tttgagaata aagtttcaac tcacggacaa ccacttacaa acgcgggtgc
      121 atcaatcgaa aatacaatcg tcaacttagg gggtgatcct caaaatccat tttgcatatt
      181 ccctgaagtt gctgctttat acgaaaaaag aaagaatgaa ttagctgaaa ttgttgcaaa
      241 acgtaaagct gaagcagcaa gttggaagaa agaaaatccg gaattgagcg agaagtatac
      301 taaattctac gataagtcat tctcaaaaat aaacttctct gagattcaac ataaattaga
      361 tcaatcaaca cgttcagctt ccggctcgat gttatctttg ttttccggta aaattgaaaa
      421 catgattgtt gcatctgccg atttatctaa ttcagataaa acagacggat tcttaaaaaa
      481 tagcaaacct ttagctaaag gcgatttttc aggaggtttc ttaaatgcag gtgtaagtga
      541 gcttacaatg gcttgtatta tgaatggatt ggcattacat ggaggtgtta ttccggtatg
      601 tggtacattc tttgttttct ctgattacat gaaacctgca ttcagaatgt ctgctcttat
      661 gcgtttacaa gtaatttacg tatggtcgca tgatgcattt agagtaggtg aggatggtcc
      721 tacacatcaa cctattgaac aagagctaca agttcgtttg atggaatctt taaagaacca
      781 taaaggtaaa aattcaatgt tggtattgcg ccctgcagac gaaagcgaaa ctactgttgc
      841 atggcagatg gcattagaaa atactgattc tccaacaggg ctaatattgt ctagacaaaa
      901 tattaaaagt ttgcctagac ctgcagatcg ttatgctgat gctttaaata tgaagaaagg
      961 agcttacgtt gttaaaaatg tagagaaacc ggatgtaata ttattagcaa gcggttctga
     1021 agttgcaaca ttatttgagg ctgctaattt attggaggat aagaaaaatc tgaaagttaa
     1081 aatcgtatca gttcctagcg aaggtgtata ctttacacaa tctgaaagtt atcgtagcac
     1141 tgttatagac tataatattc ctatttttgg tttaaccgcc ggtctaccta tgacattgca
     1201 acgtattgca ggcttaaaag ggcgcgtatt cggcttagat catttcggac attctgctcc
     1261 atataatgtg ttagatgaaa aattcggctt tacagctgaa aatatttata atcaggtttg
     1321 tgattattta gctaactact aatggatata attaccattg tattaattgc cattggctta
     1381 tctatggatt gttttgcggt atctattgca aaagcaggga cattaaataa gtttgatttc
     1441 ggtaagtttt ttgcaatggc tctgttattc ggcgcttttc aaggttttat gccttttatt
     1501 ggttacttgc tcggatataa ttttgctgcg tatatcgaaa gtgttgatca ttggcttgct
     1561 ttattgattc tatcaataat tggaggcaaa atgatttttg aagatctaaa acctaagaat
     1621 atcaacgaag atattgcaga gaaaaatcct tattcgatac aaacattgtt acttttatcg
     1681 gtagcaacaa gtattgatgc acttgcaacc ggtttggtat ttattccata taaagagtgg
     1741 ttgtggatag ctatttcagt aatcgcaatt gtaagttttg tgttttcatt tgccggttca
     1801 atgctgggtt tgttttttgg taagaaattt tctttcaaat ttggtatcgt cggcggaata
     1861 attttaatag ctattggtgt aaaaatc
//
LOCUS       DAOPID010000244         1820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_92110_length_1820_cov_3.411331, whole genome shotgun sequence.
ACCESSION   DAOPID010000244 DAOPID010000000
VERSION     DAOPID010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92110_length_1820_cov_3.411331"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="PK641_06915"
     CDS             complement(<1..948)
                     /locus_tag="PK641_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HPG56107.1"
                     /translation="MAIIKPFKGIRPPKAMVEKVASRPYDVLNSEEARKEAEGNEKSL
                     YHIIKPEIDFPAGTDEHDAKVYEKAAENFKKFQDKGWLVQDKEDLYYVYAQTMNGRTQ
                     YGLCVCAYVDDYMTGKIKKHELTRRDKEEDRMKHVRVNNANIEPVFFAYPHNDELDAI
                     VAKVCSKPAEYDFVSADGFGHHFWIIDDKNSIARITEIFGNIPSLYIADGHHRSAAAA
                     LVGDEKRKQNPNHTGKEEYNYFLAVCFPDNQLNIIDYNRVLKDLNGLSTEQFLNALKE
                     NFIVEEKGAEIYKPNKLHNFSIYIAGKWYSLTAKIGTYND"
     gene            1207..1557
                     /locus_tag="PK641_06920"
     CDS             1207..1557
                     /locus_tag="PK641_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG56108.1"
                     /translation="MKCNSDRSCPAMPCPNLTEEQRKALDEIVEKWEKFDELSESEQK
                     ELIMMHKAFVDQREAEMKAKKEAFEAAWANFDNLSIKEQKALLEAKCCCPKGGCPQQG
                     PRPEAPRGGTCPNK"
BASE COUNT          557 a          293 c          319 g          651 t
ORIGIN      
        1 atcattgtat gtaccaatct ttgcagttag tgaataccat ttaccggcta tgtaaattga
       61 gaaattgtgt agtttgtttg gtttgtatat ttctgcacct ttttcttcta cgataaaatt
      121 ctcttttaag gcatttaaaa attgttctgt gctcaatccg ttcaagtctt ttaaaaccct
      181 attatagtct ataatgttta attgattgtc ggggaaacaa acagctaaga aataattgta
      241 ctcttcttta cccgtgtgat taggattttg tttgcgtttt tcatctccaa ctaacgcggc
      301 agctgcacta cgatggtgtc cgtctgcaat gtaaagtgaa ggtatgttac cgaatatttc
      361 ggttatgcga gcaatgctat ttttgtcatc aataatccaa aaatgatgtc caaaaccatc
      421 tgcgctaaca aaatcgtatt ctgcaggttt tgaacatact tttgcaacaa ttgcatcaag
      481 ttcgtcatta tgtggatatg caaaaaatac aggttcaatg tttgcgttgt taacacgtac
      541 atgtttcatg cggtcttctt ctttgtctct acgtgttaat tcatgttttt taattttacc
      601 tgtcatatag tcatctacgt atgcacatac gcataaacca tattgggtgc gaccattcat
      661 tgtttgcgca tatacgtaat ataaatcttc tttatcttgt acaagccatc ctttatcttg
      721 gaacttttta aaattctctg ctgctttttc gtacacctta gcatcgtgtt cgtctgtacc
      781 tgccggaaaa tcgatttccg gtttaataat atggtacaat gatttttcgt taccttcagc
      841 ttctttacga gcttcttctg agtttaaaac atcgtatgga cgtgatgcaa ctttttccac
      901 catagccttt ggtgggcgga tacctttgaa tggtttaata atagccatat attaatatta
      961 attttgataa taaactttaa tgtccggcaa atttacgaaa ataatgtacc tatatcaata
     1021 cgtaggcaga ttttttttat acctttggca ttgtacttaa taatttaata aacattttaa
     1081 agtatgaaga agatttattt ggttattgtt ggagcagttt gtgtcctata aatggctgct
     1141 tcatgttgca attgttgcaa taacgatgaa aagaaatgtt gttttaaaga ttgtaaagga
     1201 ccaaaaatga aatgtaattc cgacagatca tgtccggcaa tgccatgtcc taaccttaca
     1261 gaagaacaac gtaaagcact tgatgagatt gttgaaaagt gggagaaatt tgacgaacta
     1321 tctgaaagtg aacaaaaaga gttgataatg atgcataaag cttttgttga tcaacgtgag
     1381 gctgaaatga aagctaaaaa agaagccttt gaagctgctt gggcaaattt tgacaattta
     1441 tctattaaag aacaaaaagc tttattggaa gcaaaatgtt gttgtcctaa gggaggatgt
     1501 ccacaacaag gacctagacc tgaagctcca cgtggcggta cttgtccaaa caagtaaaaa
     1561 agcctatttt taatctttta ataagccttg ctcaatgagt aaggcttatt ttttttgcat
     1621 gaataataat ttattggaaa cgtttgttta aatctgtgaa ttttttgtaa cttgcgatgt
     1681 tttgtctgaa aacacatcag attaactatt aacctaaaat ctaaatatta atgaagagga
     1741 atcattgcat attattggta ctatgtttat gcctatttca aaatcttgtt gcaatttcac
     1801 aaaatgttgc tgaattagat
//
LOCUS       DAOPID010000245         1809 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_92934_length_1809_cov_2.815279, whole genome shotgun sequence.
ACCESSION   DAOPID010000245 DAOPID010000000
VERSION     DAOPID010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92934_length_1809_cov_2.815279"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PK641_06925"
     CDS             complement(<1..414)
                     /locus_tag="PK641_06925"
                     /inference="COORDINATES: protein motif:HMM:NF027798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,3-glucanase family protein"
                     /protein_id="HPG56109.1"
                     /translation="MKLSHLFFTACLCLFSVFARAEETFPFEFLNNSVFSDDQIYIAM
                     VGKQNSTDIWIDFSENTSANPAIKPMNASYNTIHKTAGDWGYADIFTKLSDIKSKIVY
                     MPKVHACRMFISFNQPLYIHFHATGGYAGVDLNNPS"
     gene            complement(565..1002)
                     /locus_tag="PK641_06930"
     CDS             complement(565..1002)
                     /locus_tag="PK641_06930"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HPG56110.1"
                     /translation="MVTVESFTMDHTKVKAPFVRKCGIQIGSNGDIVTKFDLRFTQPN
                     KESIPTAAIHALEHLLAGYIREEIQGVIDLSPMGCRTGFYLIVWDEHDIKEVQKALIS
                     ALNKVLLATEIPAANDIQCGNYRDLSLFGAKEYAKYVIQGFDA"
     gene            complement(1156..>1809)
                     /locus_tag="PK641_06935"
     CDS             complement(1156..>1809)
                     /locus_tag="PK641_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG56111.1"
                     /translation="VYNEDPELANSAIVSFTDFIGEKPLTEGAIITVQWNVFWSSNSG
                     NFFTGVFDYKIGDYGQADIVAPTLPNLQNTGTSVTWPPCFDALSEVEKYVVNIDGDDV
                     ATILDLNEESYEYTLPETFTTMSVKAYDFAGNYSESQLPTSVCETANLNMLIISSNNG
                     FNVVGVEVKALDLCDLTGKTIASSDNAIIQCNKAGVYLLKVTTADGNVVIEKVLKAK"
BASE COUNT          613 a          344 c          268 g          584 t
ORIGIN      
        1 tgacggatta ttcaaatcaa ctcccgcgta gccacctgtt gcgtgaaagt gtatatataa
       61 aggttgatta aacgagataa acatacgaca agcatgaact tttggcatat acacaatttt
      121 agattttata tcactaagtt ttgtgaatat gtcagcgtat ccccaatctc ctgcagtttt
      181 atgaatcgta ttataactgg cattcatagg ctttattgcc ggatttgctg aagtattctc
      241 agaaaaatct atccatatat cggtactatt ttgttttccg accatagcaa tataaatttg
      301 atcgtcagaa aaaacagaat tattaagaaa ttcaaaaggg aaagtttctt ctgctctagc
      361 aaagacagaa aacaggcata ggcatgccgt aaaaaataaa tgacttagtt tcatttttat
      421 ttggtattaa gattcggttg caagttatga atttataaca taccgcaaac gtttcccact
      481 acatctaaaa tactacaatg aaaaaatgtg gattataact atgaaaacat acaatacatc
      541 aatagtacat caatcggaga ttaatcaagc atcaaaacct tgaattacgt actttgcata
      601 ctctttagct ccaaataaag ataggtctct gtagtttccg cattgtatat cgtttgcagc
      661 aggtatttct gtcgccaata ataccttatt taatgcagaa attagagctt tttgtacttc
      721 ttttatgtcg tgctcgtccc atacaataag atagaagcct gtacgacaac ccattggaga
      781 taaatcaatc acgccttgta tttcctctct tatataaccg gctaaaagat gttctaatgc
      841 atgaatagca gcagtaggaa tagattcttt attcggttgc gtaaagcgta agtcaaattt
      901 tgttacaata tcgccattgc tacctatttg aataccacat ttacgaacaa agggagcttt
      961 aacctttgta tgatccatcg taaaactttc tactgttacc atatacttaa atttaattat
     1021 gcaaaattat cgaaaataat cgaaaagcat acatgattta taatatttgc gcttaacaaa
     1081 aagagtccgg cataactgcc gaactcttta aagaatcgaa caagcattcg attcaaacta
     1141 ttaatgtaac acaaatcact tagctttcaa aactttttct attacaacat tcccatcagc
     1201 agttgtaacc ttcaacaaat acacacctgc cttattacat tgaattattg cattatctga
     1261 agatgctatt gtcttgccgg tcaagtcaca taaatcaagt gcttttactt ctacacccac
     1321 cacattgaaa ccattatttg aacttataat aagcatgttt agatttgcag tttcgcaaac
     1381 tgatgttgga agttgagact cgctataatt acctgcaaag tcataagctt tcactgacat
     1441 cgttgtaaat gtttcaggca aagtatattc ataactttct tcatttaaat ctaatatcgt
     1501 tgctacatcg tcgccatcta tgtttactac atatttttca acttcactaa gagcatcaaa
     1561 gcaaggaggc catgttacag aagtacctgt gttttgtaag tttggaagtg taggagccac
     1621 aatatctgct tgaccataat cacctatctt ataatcaaaa acccctgtaa agaagttacc
     1681 actattactt gaccaaaata cgttccattg aacagtaata atagcaccct ctgttagtgg
     1741 cttttcacct ataaaatcag taaatgatac aatagcagaa tttgcaagtt caggatcttc
     1801 attataaac
//
LOCUS       DAOPID010000246         1801 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_93529_length_1801_cov_3.097365, whole genome shotgun sequence.
ACCESSION   DAOPID010000246 DAOPID010000000
VERSION     DAOPID010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93529_length_1801_cov_3.097365"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /locus_tag="PK641_06940"
     CDS             complement(<1..1119)
                     /locus_tag="PK641_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HPG56112.1"
                     /translation="MKNKYVDLIEQTSEFPTEEFQVKDNELQFNGIPLMDVIEQYGTP
                     LRLTYLPKIAQQIQKARRMFNVAMAKVDYNADYHYCYCTKSSHFSFVMEEVLKHGVHI
                     ETSSAFDINLIEDLYETGSFKKDNYIICNGFKRPQYVENIQHLVNEGFENTVAILDNK
                     FELDLLLNGLDKKCKVGIRIASEEEPKFDFYTSRLGIRYNDILPYYYEKIKDNPKTEL
                     KLLHFFINTGIKDTAYYWSELVKAVELYCELKKICPELDSLDIGGGFPIATYLDYNYD
                     YEYMAEEIVAQIKTICSQNGVQEPNIFTEFGSFTVGESGAIIYSIVNQKQQNDRELWN
                     MIDSSFITTLPDTWSINQRYILLAINNWDKEYQRVHLGG"
     gene            complement(1291..>1801)
                     /locus_tag="PK641_06945"
     CDS             complement(1291..>1801)
                     /locus_tag="PK641_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HPG56113.1"
                     /translation="NSGMVVEIRPEDLPEYAKYGVFSMMRFQEDLERKAWLSGGSTQV
                     APAQRMTDFIHKKESSTLPDSSYVPGLKSTQLNTVLPRFIDARLRDGLATFGKWSSNF
                     LTNEAALIGVETRTSAPIRVLRDTETLAHIQIEGLYPCGEGAGYAGGIVSAAIDGERC
                     AEMCAAATL"
BASE COUNT          579 a          352 c          247 g          623 t
ORIGIN      
        1 tcctccaaga tgtacacgtt gatattcttt gtcccaatta tttatagcca ataaaatgta
       61 tcgctgatta attgaccatg tatcaggcaa agtagttata aatgaactgt ctatcatatt
      121 ccataactca cgatcattct gttgcttttg gtttactata ctataaataa tagcaccgct
      181 ttcaccaaca gtaaacgacc cgaactctgt gaatatattc ggttcttgaa ctccgttctg
      241 tgaacatatt gttttaattt gtgctacgat ttcttctgcc atatattcat aatcgtagtt
      301 ataatccaaa tatgtagcta ttgggaatcc tccgcctata tccaaactat caagttccgg
      361 acaaatcttt ttcaattcgc aatacagttc taccgctttt accaattcac tccaataata
      421 agcagtatct ttaattcctg tattgataaa gaaatgcaat aatttaagtt ccgttttagg
      481 attatctttt atcttttcat aataatatgg aagaatgtca ttatatctga tacctaaacg
      541 agatgtataa aaatcaaact ttggttcttc ttcagatgca atacgaatac ctaccttaca
      601 ttttttatct aaaccattaa gtaatagatc caattcaaat ttattatcta atatagcaac
      661 ggtattttca aaaccttcat ttacaagatg ttgaatattt tcaacatact gcggacgttt
      721 aaatccgtta catataatat aattatcctt tttaaatgaa ccggtttcgt acaaatcttc
      781 aatcagattt atatcaaaag cagaagatgt ttcaatatgt acaccatgtt tcaacacttc
      841 ttccattaca aatgaaaaat gtgaactctt agtacaatag caataatgat agtcggcatt
      901 ataatcaacc ttagccattg ctacattaaa cattctgcga gctttttgta tttgctgagc
      961 tattttaggc agatatgtaa gccttagtgg tgtaccatac tgttctatta catccatcaa
     1021 tggtatccca ttaaactgca actcattatc cttaacttga aactcctctg tagggaattc
     1081 tgatgtctgt tcaataagat caacgtactt gtttttcatt atagttacaa atttaactta
     1141 cttactttgt gttttttttg acaatgtaac tcactcggat tgtaaaaatc attaaagttg
     1201 acaaagataa ataataatat gtaccactat aactgttttt ataaaaaaaa cgatgatagt
     1261 atataaaaaa actatatgtt ttaactaaaa ctataatgta gcagcggcac acatctctgc
     1321 acaacgttct ccatctattg cagccgaaac aattccgcct gcatatccgg ctccttctcc
     1381 gcaaggatat aagccttcta tttgtatatg tgccaatgtt tctgtatcac gtaatacacg
     1441 aattggagct gatgttcttg tttcaacacc tatcaaagca gcttcattag ttaagaaatt
     1501 tgacgaccat ttaccaaaag tagctaagcc atcacgcaaa cgagcatcta taaaccttgg
     1561 taaaacagta tttaactgtg tagatttaag tccgggtaca taagaactat cgggcaatgt
     1621 agaagattct tttttatgta taaagtcggt cattctttgt gccggagcta cttgtgtgct
     1681 tcctcctgac agccatgcct ttctttctaa atcttcttgg aaacgcatca tcgaaaaaac
     1741 accatattta gcatattcgg gcaaatcttc cggacgtatt tccactacca tacccgaatt
     1801 a
//
LOCUS       DAOPID010000247         1783 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_94874_length_1783_cov_3.880787, whole genome shotgun sequence.
ACCESSION   DAOPID010000247 DAOPID010000000
VERSION     DAOPID010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94874_length_1783_cov_3.880787"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1418)
                     /locus_tag="PK641_06950"
     CDS             complement(<1..1418)
                     /locus_tag="PK641_06950"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005820521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPG56114.1"
                     /translation="MSKTKYIFVTGGVTSSLGKGIISASIGKLLQSRGYSVTIQKFDP
                     YINVDPGTLNPYEHGECYVTVDGHETDLDLGHYERFTDIETHSANSITTGRIYLNVIN
                     KERHGDYLGRTVQIIPHITDEIKRNILLLGQKNNLDFVITEIGGVAGDIESLPFLESV
                     RQLKWELGTDCICVHLTYVPYLAAAKELKTKPTQHSVKKLQELGIQPDILVLRTEHDI
                     NEEIRRKVSQFCNVLPEAVIQSKDVSTIYEVPLKIQEQHIDSIILKKVGLSPDDTKSD
                     LTKWKSFLNMLKSSNEIVEIGLVGKYVELQDAYKSISEALFQAATYNKHKLKLVYIHS
                     ENIDAINVEEQLKDMDGIVIAPGFGQRGIEGKFVALKYAREHNIPTFGICLGMQCMVI
                     EYARNVLGLHQANSTEMDPKTKQNVIDLMESQKAITDKGGTMRLGAYRCDLVPNSLVL
                     KAYEKSTIYERHRHRYEFNNKY"
     gene            complement(1447..>1783)
                     /locus_tag="PK641_06955"
     CDS             complement(1447..>1783)
                     /locus_tag="PK641_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPG56115.1"
                     /translation="KVWDFENDKDAIKLKSKVPVILDFYADWCRPCKMLEPELTALQK
                     DYKGKLIIYRINVDKERNLAALFQASSLPTIFFIDKSGTATYVRGYRTKEELKQMVDT
                     YLITAKKAK"
BASE COUNT          544 a          338 c          249 g          652 t
ORIGIN      
        1 ttatatttat tgttaaattc gtaacgatga cgatgacgtt cgtatatagt tgatttttca
       61 tatgccttta aaacaagtga attaggtact aaatcacatc tataagcacc taaacgcatt
      121 gtacctcctt tatctgttat agctttttga gattccatta agtcaataac attttgcttg
      181 gttttaggat ccatttctgt tgaatttgct tgatgcaatc ccaaaacatt acgtgcatat
      241 tcaataacca tacattgcat acctaaacat ataccaaaag taggtatatt gtgttcacga
      301 gcgtatttca atgcaacaaa cttaccttca atgccacgtt gaccaaatcc aggagcaata
      361 acaataccat ccatatcttt taattgctct tcaacattta tagcatctat attctcagaa
      421 tgtatatata ctaattttaa tttatgttta ttatacgtag cagcttggaa taatgcctca
      481 ctgatagatt tatacgcatc ttgtaattca acatacttac ctaccaaacc aatttcaact
      541 atctcattag atgattttaa catattaagg aatgacttcc atttagttaa atctgattta
      601 gtatcatccg gtgatagacc gactttcttt aaaattatgc tgtctatatg ttgctcctga
      661 atttttagtg gaacttcata tatagtagaa acatctttgc tttgaattac agcttccggc
      721 aacacattgc agaattgaga tactttacgc ctaatctctt cattaatatc atgttctgtc
      781 ctaagtacta aaatatcagg ctgaatgcct aattcttgca acttcttaac agaatgttgt
      841 gtaggcttag tttttaattc tttggcagca gctaaataag gtacgtatgt cagatgaacg
      901 caaatacaat cagttccaag ttcccacttt aattggcgta cactttccaa aaatggaaga
      961 gattctatat cgcctgcaac accaccgatt tctgtaatta caaagtccaa attattcttc
     1021 tgtcctaaca acaaaatgtt tcgtttaatt tcatctgtaa tatgaggaat aatctgaact
     1081 gttcggccta aataatcgcc atgacgttct ttgtttataa catttagata tattctaccc
     1141 gtcgtaatac tgttggcgct atgtgtttct atgtccgtaa aacgctcata atgtcctaag
     1201 tccaaatctg tttcgtgacc atctactgtt acataacatt caccatgttc gtatggattc
     1261 aatgtacccg gatctacatt tatatatgga tcaaattttt gaatcgttac actatatcca
     1321 cgcgattgta gtaatttccc aatcgaagcg gatattatac ctttacctag tgaagaagta
     1381 acccctcctg ttacgaaaat atactttgtc tttgacatat gatataagtt aggtaaattc
     1441 taatgcttat ttagcttttt ttgccgtaat tagatatgta tctaccattt gtttaagttc
     1501 ttcctttgtg cgataacctc ttacgtatgt agcagttcct gatttgtcta taaagaaaat
     1561 tgttggtagc gaactagctt gaaataatgc agccaaatta cgttccttat ctacatttat
     1621 tctgtatatt atcaatttac ctttataatc tttttgtaaa gctgttaatt caggctctaa
     1681 cattttgcat ggacggcacc aatctgcata aaaatctaat attacaggca ccttactttt
     1741 aagtttaatg gcatctttgt cattttcaaa atcccaaact ttt
//
LOCUS       DAOPID010000248         1718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_100358_length_1718_cov_3.098016, whole genome shotgun
            sequence.
ACCESSION   DAOPID010000248 DAOPID010000000
VERSION     DAOPID010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100358_length_1718_cov_3.098016"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /locus_tag="PK641_06960"
     CDS             complement(<1..1149)
                     /locus_tag="PK641_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPG56116.1"
                     /translation="MRLITYVIGVVVLCGIPDAILFRLFYKWKNKTLIRLQWISMALF
                     LAVFAYAMVAIRFTVSMIPMLAFGFVFFLIYLPKWFFWPFALAGHLKIGSIFAAIAAC
                     LILYGTIYGRRDIVTRQIDIYSDKIPESFDGYRIAMFSDIHLGSLSKTSKWINALPDS
                     ISKNNPDIVLFTGDLVNMYAKETNGWDNMFLRINAPDGKYGVMGNHDYSVYLKNRSIK
                     ADSAINTDNIRNAFNRFGFTLLNDKNIFLRRGNDSIALIGTENIGNPPFPVIGNIDEA
                     LSGTENTKCKIMMTHDPNIWDDIIVDKRNVLLTLSGHSHALQMGFDHLGIHWSPAQFI
                     HEYWDGPYQKKDQILFVNRGMGYVGYPFRIGMKPEITLIILHPSKKHGT"
     gene            complement(1128..>1718)
                     /locus_tag="PK641_06965"
     CDS             complement(1128..>1718)
                     /locus_tag="PK641_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009041136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPG56117.1"
                     /translation="DEIEKAHPDVFNLLLQVLDEGQLTDNVGRTVSFKNTIIIMTSNI
                     GTRQLKEFGNGVGFTTASRAADSEYASSVIKKALQKTFSPEFLNRIDDIIVFDQLSKE
                     SIIKIVDIELKNVVNRIENLGYKLNLTDAAKSFIADKGYDVQYGARPLKRTLQKYVED
                     EIAELMVKTDSIPDGSTINIDYNSETKEIECDLLHM"
BASE COUNT          548 a          328 c          251 g          591 t
ORIGIN      
        1 tgttccgtgt ttcttcgagg gatgtaaaat aattagagta atttcaggct tcattccgat
       61 tctaaatgga tatcctacgt atcccatacc tctgtttaca aacaatattt gatccttttt
      121 ttggtaaggt ccgtcccaat attcatgaat aaactgtgcc ggtgaccaat gaatacctaa
      181 atggtcaaaa cccatttgta gcgcgtgcga atgccctgaa agtgtaagca aaacatttct
      241 cttatcaaca ataatatcat cccaaatatt tggatcgtgc gtcatcatga ttttgcattt
      301 ggtattttca gtaccgctta atgcttcatc tatatttcca ataacaggga atggtggatt
      361 tcctatattt tcagtaccaa taagagctat cgaatcattt ccacgcctca aaaatatatt
      421 tttatcattt aaaagagtaa acccaaatct attaaatgca ttacgtatat tatcagtatt
      481 aatagccgaa tctgctttta tacttctatt tttaagataa acactataat cgtgattacc
      541 cattactccg tattttccgt caggcgcatt tatacgtaag aacatattat cccatccatt
      601 agtctctttt gcatacatat taaccaagtc ccctgtaaat aatacaatgt caggattatt
      661 tttggatatg ctatccggta aagcattaat ccattttgat gttttactta agctacctaa
      721 atggatatct gaaaacatag caattctata accatcaaat gattccggaa tcttatctga
      781 gtatatatca atttggcgcg taactatatc acgtctacca taaatagtgc cgtataatat
      841 aagacatgcg gctatggctg caaaaataga gcctatttta aggtgtccgg ctaatgcaaa
      901 tggccaaaag aaccatttag gtaaatatat taagaagaag acaaaaccga atgctagcat
      961 tggtatcata ctgactgtga atcttatagc aaccattgcg tatgcaaaca cagccaaaaa
     1021 tagagccata ctaatccatt gtagtctaat taatgtctta tttttccact tataaaatag
     1081 tcgaaataat atggcatcag gtattccaca cagtacaaca actccaatta catatgtaat
     1141 aagtcgcatt ctatttcttt ggtttctgaa ttataatcaa tatttattgt actgccgtca
     1201 ggaatagagt ctgttttaac catcagttct gcaatctcat cttcaacata tttttgcaat
     1261 gttcttttta gtggacgagc cccatactga acatcgtatc ctttatctgc aataaaactt
     1321 tttgctgcat cagtaagatt tagcttatat ccgagatttt ctatacgatt aacaacattt
     1381 ttaagttcta tatccacaat tttaataata gactcttttg ataactgatc aaaaacaatt
     1441 atatcatcga ttctatttaa aaattcgggt gagaatgtct tttgtaatgc tttttttata
     1501 acacttgatg catattcgct atctgctgca cgagaagcag ttgtgaatcc gacaccattt
     1561 ccaaactctt tcaattggcg tgtacctata tttgatgtca tgataatgat ggtgttcttg
     1621 aaactaactg tacggcctac attatcagtt agttgacctt catccaagac ttgtaacaac
     1681 aagttaaaca cgtcaggatg agctttttca atttcatc
//
LOCUS       DAOPID010000249         1697 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_102156_length_1697_cov_3.927527, whole genome shotgun
            sequence.
ACCESSION   DAOPID010000249 DAOPID010000000
VERSION     DAOPID010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102156_length_1697_cov_3.927527"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="PK641_06970"
     CDS             complement(<1..551)
                     /locus_tag="PK641_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HPG56118.1"
                     /translation="MNAQNVVEGLKKRFPNEPEYIQAVSEVLNSIEEEYNKHPEFEKA
                     NLIERLCIPDRIFTFRVSWVDDKGNVQTNMAYRVQHNMAIGPYKGGIRFHSSVNLSIL
                     KFLAFEQTFKNALTTLPMGGAKGGSDFSPRGKSNNEVMRFCQAYLLELARHIGPNTDI
                     PAGDIGVGGREVGFMFGMYRKLQ"
     gene            871..1263
                     /locus_tag="PK641_06975"
     CDS             871..1263
                     /locus_tag="PK641_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG56119.1"
                     /translation="MFKNCVISTKAKVISIISAVLLLTLAGFIYFRFYFVFGEGVKAG
                     ELNYCTYKGYIFKTYEGKLIQSGFKAQSIGTIQSYEFPFSVENKLIADSLMRCSGRHV
                     ELHYKEYIAPIPWRGVSKFVVDDIISVK"
     gene            1383..>1697
                     /locus_tag="PK641_06980"
     CDS             1383..>1697
                     /locus_tag="PK641_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPG56120.1"
                     /translation="MELESKYKPSEVEPKWYQYWLDNGMFNSKPDNREPYTIVIPPPN
                     VTGVLHMGHMLNNTIQDILVRRARMLGKNACWVPGTDHASIATEAKVVNRLAQQGIKK
                     TDL"
BASE COUNT          556 a          262 c          291 g          588 t
ORIGIN      
        1 tgttgtaatt tgcgatacat gccgaacatg aaaccaactt cacgaccacc aacacctata
       61 tcacctgcag gtatgtcagt gttagggcct atgtggcgag ctaattcaag caaataagct
      121 tggcaaaaac gcatcacttc gttgttagat ttacctcttg gtgagaaatc cgaaccgcct
      181 tttgcgccac ccataggcaa tgttgtaagt gcgtttttaa atgtttgctc gaaagcaagg
      241 aattttaaaa ttgacaagtt aaccgaagag tggaaacgaa taccaccttt ataaggtccg
      301 atagccatgt tgtgttgtac acgatacgcc atattggttt gtacgtttcc tttatcatct
      361 acccatgata cgcggaatgt aaaaatacga tccggaatgc aaagtctctc aattaaatta
      421 gctttttcaa actcgggatg tttgttgtac tcctcttcga ttgaatttaa tacttcactt
      481 acagcttgaa tatattcagg ttcatttgga aaccttttct tcaagccttc tactacattt
      541 tgtgcattca taattatgtt gtttattaga aataacttat gttttgagtt cttttgatgt
      601 gcaaagttaa acaaaaaaat agttgaagca caaaagccat aaataaaata gatacaaaaa
      661 aacgatatag tgcaatttgt tatattttta ctattaaaac taccacattg taattaattg
      721 caattatttg cttataaatt gatattttgg tgtttctaaa aatacgatta ttgtgaaaaa
      781 gatatcattt atatttttat tgtgcataat tttcttaatt ttgctgttca aagcatttgt
      841 ctctaaattt atatttatac ctattatatt atgtttaaaa attgcgttat tagtacaaag
      901 gcaaaagtta ttagcataat ttctgccgta ttgctcctga cattagcagg atttatatat
      961 ttcagatttt attttgtatt tggagaaggt gtaaaagctg gtgaacttaa ttactgtact
     1021 tataagggat atatatttaa aacatatgaa ggaaaattga ttcaatcggg ttttaaagca
     1081 caatcaatag gaaccataca atcatatgaa tttccttttt ctgtagagaa taaattaatt
     1141 gctgattctt tgatgcgctg tagtggacgt catgtagaac ttcactataa ggagtatatt
     1201 gctccgatcc cttggcgagg agttagcaag tttgttgtag atgatatcat ttcagtaaaa
     1261 taattttttt gctatttagc aatcccaact caaaagagtt gggatttttt ttattatatg
     1321 atatacatta tgtaataaca ttttatgtac ctttgtcctt tcatattttt agagaccttt
     1381 ttatggaatt agaaagcaaa tataagcctt cagaagttga accgaagtgg tatcagtatt
     1441 ggttggataa tggtatgttt aattcgaaac cggataatcg cgaaccctat accatagtaa
     1501 tacctccacc aaatgtaaca ggtgtcttac acatggggca catgcttaat aataccatac
     1561 aagatatttt agtaagacgt gccagaatgt tagggaaaaa tgcatgttgg gtaccaggta
     1621 cagatcatgc atcaattgca acagaagcaa aagttgtaaa tagattggct caacagggta
     1681 ttaaaaaaac agatctt
//
LOCUS       DAOPID010000250         1678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_103884_length_1678_cov_3.337646, whole genome shotgun
            sequence.
ACCESSION   DAOPID010000250 DAOPID010000000
VERSION     DAOPID010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103884_length_1678_cov_3.337646"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..1658
                     /locus_tag="PK641_06985"
     CDS             192..1658
                     /locus_tag="PK641_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family protein"
                     /protein_id="HPG56121.1"
                     /translation="MYNNVLILLDNLDDWKPYYDTNSVMSVSNYLQAQLIGKERKLII
                     NLSNDYTYNSEGYYCSLLAQTRGHQVLPDIETINRLKIGSFIRMPASLQKVCYQWIQS
                     QNIQTETWTLNIYFGKCAEAGLERIARFIFENFPCPILNVTFNNHSKNQIESIQALGI
                     NMLNESEQDCFAAALDTFNKKIWRIPRSSKPARYNLAILYDPEEKFPPSNKQAINKFL
                     EIAKKMDIRAELVTEEDITRLMEFDALFIRTTTSLNHYTFHWSQTARQNGLAVIDDPV
                     SIIRCTNKVYLNELFYKEHIPAPKSTLLFKSNPKTFDEIAEILGAPFILKIPDGSFSI
                     GMYKIENQKALDDALNVLYVHSSIILAQAFIPTEFDWRVSILNGEPIYVCKYYMAKNH
                     WQIYHHYKSGKSRCGIVETLPIYKVPRKIIDTALKAANLIGKGLYGIDLKTVDDKVLV
                     IEINDNPSIDHGCEDIVLGDELYYRILNYFEREVDKNR"
BASE COUNT          614 a          273 c          271 g          520 t
ORIGIN      
        1 tggaaagtat atatactaca tcaatacttc aactacttta tttctttcaa ttctctcctt
       61 aacagcttat taatcgtttt gaatttcgta tgaattgaac taagacgcaa tgaactttga
      121 taaaataaaa attttttgaa ccacgtatgg cttttctatt tatcttcgca ccgtcaaatt
      181 tgtaatatat tatgtataat aatgttctta ttttgttaga caatcttgat gattggaaac
      241 catactatga caccaatagt gttatgtctg tatcaaatta cttacaagca cagctaattg
      301 gtaaagagcg gaaacttata attaatttga gcaatgacta cacttataat tcggagggtt
      361 attattgttc tttattagct caaacacgcg gacaccaagt tttaccggac attgagacaa
      421 taaacaggct caaaatagga tcattcatta ggatgcccgc atcactgcag aaagtatgtt
      481 accaatggat acaaagtcaa aacattcaaa ctgaaacttg gacacttaat atttattttg
      541 gtaaatgtgc agaagccgga ttggaaagaa tcgcacgttt tatttttgag aatttcccat
      601 gccccatttt aaatgtcaca tttaataacc atagcaaaaa tcaaattgaa agcattcaag
      661 cactaggtat taatatgcta aatgaatcag agcaagattg ttttgctgct gcacttgata
      721 catttaataa gaaaatatgg aggattccca gatcttctaa gccggctaga tataatttag
      781 ctatactcta cgatcctgaa gagaaatttc cgccaagcaa caaacaagca ataaacaaat
      841 tccttgagat tgcaaaaaaa atggatatta gagcagaact tgttaccgaa gaggatataa
      901 caagattaat ggaatttgat gctttgttta taaggactac aacatcgttg aatcattata
      961 catttcattg gtctcaaaca gcaagacaaa acggactggc agtaattgac gaccctgttt
     1021 caataattcg ttgtacaaat aaggtctatt taaacgaact tttttacaaa gaacatattc
     1081 cggcacctaa atcgactttg ttatttaaat ctaatcccaa aacttttgat gaaatcgcag
     1141 aaatattagg agctccattt attttaaaaa tacctgatgg atctttctct attggtatgt
     1201 acaagattga aaatcagaaa gcattagacg atgccttaaa tgtattatat gttcattcat
     1261 ctattatact ggcacaagcc tttataccta ccgaattcga ttggcgtgta agtatattaa
     1321 atggagaacc tatttacgtg tgcaaatatt atatggctaa aaaccattgg caaatatatc
     1381 atcactataa gtcaggaaaa agcagatgcg gaattgttga gacgctacct atatacaaag
     1441 taccacgtaa aataattgac acagcgttaa aagcagcaaa tttaataggt aaaggcttat
     1501 atggcatcga tttaaaaact gttgacgata aagtcttagt aatagaaatc aacgacaatc
     1561 caagcataga tcatggttgt gaagacattg tacttggaga cgaattatat tacagaatac
     1621 taaactattt tgaacgtgaa gtcgataaaa acagataaaa tgaatacatc gatggaat
//
LOCUS       DAOPID010000251         1667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_104888_length_1667_cov_4.949132, whole genome shotgun
            sequence.
ACCESSION   DAOPID010000251 DAOPID010000000
VERSION     DAOPID010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104888_length_1667_cov_4.949132"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PK641_06990"
     CDS             complement(<1..453)
                     /locus_tag="PK641_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HPG56122.1"
                     /translation="MKRIYCLFFILNCCCGLMFGNNDALIQKIQNSNGGHKSASCPFT
                     QSKHLKMMEKSVDSKGTLYVMNDKMSMIYSEPAGDFLVINGDKFVMNVRGRLIEHNVK
                     EGSPMRILRNTLLLCMKGDIKAIEAETKSEISYNGDGKYHEFTITRKEK"
     gene            complement(498..1226)
                     /gene="fabG"
                     /locus_tag="PK641_06995"
     CDS             complement(498..1226)
                     /gene="fabG"
                     /locus_tag="PK641_06995"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HPG56123.1"
                     /translation="MKYALVTGGSRGIGRAVSVKLASMGYTVIVNFVSNETEAQKTLE
                     LIREVGSNGELLKFDVADNAAACKAIETWQDAHPDNYIEVLVNNAGIRRDNLMIFMEP
                     EDWFRVLNTSLTAFYNVTRPLLKSMLVKKYGRIINMASLSGLKGLPGQTNYSAAKGGL
                     IAATKALAQEVARKKVTVNAVAPGFVKTEMVEGLDEKELSAQIPIARFGDPEEIAAVV
                     GFLASKEASYITGEVISVNGGLYT"
     gene            complement(1231..>1667)
                     /locus_tag="PK641_07000"
     CDS             complement(1231..>1667)
                     /locus_tag="PK641_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPG56124.1"
                     /translation="DSCDCLDILTPMNWNEVEAWSDKPIGKRGEDYVAMKNKKTGECI
                     KLVSQYIPELEEAVERAYTSTPLSYRNYTNTANGSAYGVRKDFNSPMLTILPAKTPIP
                     NLLMTGQNLNLHGVLGVSMTSFFTCAEIVGMDTLIKDLDINE"
BASE COUNT          480 a          335 c          267 g          585 t
ORIGIN      
        1 tttctctttg cgagtaattg taaattcatg atattttccg tcaccattgt atgaaatttc
       61 agattttgtt tctgcttcga tagctttaat atcacctttc atgcaaagta gtaatgtgtt
      121 gcgtaatatc ctcattggag aaccttcttt tacattgtgt tcaataaggc ggcctcgtac
      181 attcattaca aacttatctc cattaatcac aagaaaatca cctgcgggtt cgctgtaaat
      241 catagacatt ttgtcgttca taacatataa agttccctta gaatctacag acttttccat
      301 cattttaaga tgtttacttt gtgtaaaagg gcagcttgca cttttgtgtc caccattgct
      361 gttttgaatt ttttgaatca gggcgtcatt gtttccaaac atcagtccgc aacagcagtt
      421 aagaataaaa aacaaacaat atattcgttt cataatgtta tatatattag ttacttatat
      481 gattaattaa ttgctaatta ggtgtataat ccgccattta cagaaataac ttcacctgtt
      541 atatatgaag cttcttttga ggccaaaaag ccaacgacag ctgctatttc ttcaggatct
      601 ccaaagcgag ctataggaat ctgggcagat aattcttttt catctaagcc ttctaccatt
      661 tcagttttta caaaaccggg tgcaacagca ttaactgtaa cttttttgcg agcaacttct
      721 tgagctaatg cttttgttgc tgcaataagt ccgcctttag ctgcagaata attagtctga
      781 cccggtaaac ctttgagtcc tgataaagaa gccatattaa tgatacgtcc gtatttttta
      841 accagcatag attttaaaag cggtcgtgta acattgtaaa aggctgttaa acttgtattt
      901 aaaaccctga accaatcttc aggctccata aatatcataa gattatctct gcgaatgcct
      961 gcattattta ccaaaacttc aatataattg tcgggatgtg catcttgcca tgtttcaata
     1021 gctttgcatg cggctgcatt gtcggccaca tcaaatttga gtagttcgcc gttacttcca
     1081 acttctctaa tcaattccaa agttttttgt gcctccgttt cgttagatac gaaattgact
     1141 attacggtat aacccatgct tgccaatttt acacttactg ctctgcctat accacggctt
     1201 ccacctgtta ctaatgcgta tttcatatta ttattcatta atgtctaagt ctttaattaa
     1261 agtgtccatt cctacaattt ctgcacatgt aaaaaatgat gtcatagaga cacctaatac
     1321 gccatgtagg tttaaatttt gtcctgtcat aagcaaatta ggtataggcg tttttgccgg
     1381 taaaatcgtt agcatagggc tattgaaatc tttgcgaaca ccatatgccg aaccatttgc
     1441 tgtatttgta taatttctat aagatagagg agtgcttgta taggcacgtt caactgcttc
     1501 ttccaactca ggaatgtact gtgataccaa cttgatacat tcaccggttt ttttgttttt
     1561 catagcaacg taatcttcac ctcttttacc aataggttta tccgaccatg cttcaacttc
     1621 gttccaattc ataggagtaa ggatatctaa acaatcgcac gaatcgt
//
LOCUS       DAOPID010000252         1654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_106135_length_1654_cov_4.841151, whole genome shotgun
            sequence.
ACCESSION   DAOPID010000252 DAOPID010000000
VERSION     DAOPID010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106135_length_1654_cov_4.841151"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="PK641_07005"
     CDS             <1..1041
                     /locus_tag="PK641_07005"
                     /inference="COORDINATES: protein motif:HMM:NF012481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HPG56125.1"
                     /translation="VDAAFSNVFIDKLDACLQGRAIDYLVINHMEPDHSASIKAVKNR
                     WPNIKIVGNSKTFTMLNGYFGINENLVEVKEVSEICIGKRCLQFIMTPMVHWIETMMT
                     YEKNEGILFSGDAFGCFGALNGAVLDCDMDTEMYFPEMERYYAGIVGKYGSPVQKAIE
                     KLSNKQINMICSTHGPVWKQHVTKVIDCYNTLSLYKAKNSCVIIYGSMHGNTAKMAET
                     IADSMAAAGLKHIVMHDLSRTAPSELLADIFRCKGLILGCPTYSNELFPPMSALLDKI
                     KNREIKHRYYACFGSCSWAGTAKKKLEEFGEQMQFDSVYPAIEEKHAMKEENFEKFVE
                     MGRAMAIKLLED"
     gene            complement(1038..>1654)
                     /locus_tag="PK641_07010"
     CDS             complement(1038..>1654)
                     /locus_tag="PK641_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HPG56126.1"
                     /translation="LACNTASAKALRSIQQLDLPKLGSEKRVLGVIRPTIESINDITN
                     TKHIGIFGTEGTIQSESYPIEIHKLHPDIVVNGQACPLWVPLIENNEFNSDGADFFVE
                     KYCSNLINKDPLIDTIILGCTHYPLLMDKIRKYLPQNVSIIAQGPIIANSLKDYLTRH
                     KEMETTLSKSGSCEFYTTESVDKFTSSASVFLKDNVNAKHICLS"
BASE COUNT          535 a          246 c          345 g          528 t
ORIGIN      
        1 gttgatgctg catttagtaa tgtttttata gacaaattgg atgcgtgttt gcaaggacgt
       61 gcgattgatt atttggtaat caatcacatg gagccggatc actctgctag tataaaggct
      121 gtaaaaaata gatggcctaa tattaagatt gtaggaaatt ccaaaacatt tacgatgtta
      181 aatggctatt ttggaataaa cgaaaacttg gtagaggtaa aagaagtaag tgagatttgt
      241 ataggcaaac gttgtttaca atttatcatg acaccgatgg tgcattggat agaaacaatg
      301 atgacttacg aaaagaacga aggcatactg ttttcgggtg atgctttcgg atgctttggt
      361 gcacttaatg gtgctgtatt ggattgtgat atggataccg agatgtattt tcccgaaatg
      421 gaacgctatt atgccggaat tgttggaaag tacggttcac cagtgcaaaa agcaatagaa
      481 aaattatcga acaaacaaat aaatatgatt tgttcaacac acgggccggt atggaaacag
      541 catgtaacaa aagttattga ctgctacaat acattgagtc tgtacaaagc taaaaactcg
      601 tgtgttatta tatacggcag tatgcatggg aatacagcta aaatggcgga aaccattgcc
      661 gattcgatgg cggctgcagg attgaaacat attgtaatgc atgatttgtc gcgaactgca
      721 ccatcagaac tattggcaga tattttccgc tgtaaaggtt taattttggg ctgccctaca
      781 tactcaaacg aattattccc tcctatgtca gctttattgg acaaaattaa gaacagagaa
      841 attaagcatc gttattatgc ttgttttgga agttgctcgt gggctggaac agccaagaaa
      901 aaacttgagg agtttggtga acaaatgcag tttgattctg tttatcctgc tatagaagaa
      961 aaacatgcaa tgaaagaaga aaattttgaa aagtttgtag aaatgggtag ggctatggct
     1021 ataaaacttt tggaagatta gcttaagcaa atatgcttgg catttacgtt atcttttaga
     1081 aatacagatg cagatgaagt gaatttatcg accgattcgg tagtataaaa ttcacaactt
     1141 ccacttttgc ttaaagtagt ctccatttct ttgtgacgag tcaaatagtc ttttaaacta
     1201 ttggcaataa taggaccttg agctataata cttacatttt gaggtaggta cttccgtatt
     1261 ttatccatta ataaggggta gtgtgtacag cctaagatta tagtgtctat tagtggatct
     1321 ttgttaatta agttggaaca atatttttct acaaaaaaat ctgctccatc agaattaaat
     1381 tcattgtttt caattaaagg aacccataaa gggcaagcct gaccatttac aacaatgtca
     1441 ggatgaagtt tgtgtatttc aatagggtaa gattctgatt gaatagtacc ttctgttcca
     1501 aaaatcccta tgtgtttagt attagttata tcattgatac tttcgatagt aggtctaatt
     1561 actcccaata ctcgtttttc ggatcctaat ttgggtaaat ccaattgttg tatactgcgt
     1621 agtgcctttg cagatgctgt attacatgct aaaa
//
LOCUS       DAOPID010000253         1639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_107569_length_1639_cov_3.635732, whole genome shotgun
            sequence.
ACCESSION   DAOPID010000253 DAOPID010000000
VERSION     DAOPID010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107569_length_1639_cov_3.635732"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1639)
                     /locus_tag="PK641_07015"
     CDS             complement(<1..>1639)
                     /locus_tag="PK641_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HPG56127.1"
                     /translation="HLLLAILKDTTNLVSEMFHTQGISYNTIRNLVEKKDTLPASGSN
                     DNDKFDNDSEDDDDNYMANTVSNNGKTTDTGTYTKQSTKTPALDSFGSDITAAAAQGK
                     LDPVVGREKEIERLAQILSRRKKNNPVLIGDPGVGKSAIVEGLANHIVEHRAPMILSD
                     KRIISLDMASLVAGTKYRGQFEERLKAILKELENNDNIILFIDEIHTIIGAGATAGSL
                     DAANMMKPALARGQIQCIGATTLDEYRQSIEKDGALERRFQKVIVKPTSKEETIEILN
                     NIKERYEAHHNVIYTDEAIKACVTLTDRYINDRQFPDKAIDAMDEAGSRAHISNAVIP
                     QDIQDLEHNIAEINRLKLKAVKDEDFKLANEYKDKEKSLNISLAESKKNWEIQVRKNA
                     LVIDADKIAEVISMIAGVPVQKVEEAEFAKLRRMDSALKGLIIGQDKAVDTLCKSILR
                     NRIGLKDPNKPIGSFIFLGPTGVGKTYLTKVLSEYMFDSKNALIRVDMSEYMEKFSVS
                     RLIGAPPGYVGYEEGGQLTEKVRRNPYAIVLFDEIEKA"
BASE COUNT          446 a          340 c          261 g          592 t
ORIGIN      
        1 gctttttcaa tttcatcaaa caatactatg gcatatggat ttctacggac tttctcggtc
       61 aattgtccac cttcttcgta acctacatat cccggaggtg ctccgattaa tctcgatacg
      121 ctgaatttct ccatatattc gctcatatca actctaataa gagcattctt tgagtcaaac
      181 atatattctg ataacacttt cgtcaagtat gttttaccaa cacctgtagg ccctaaaaat
      241 ataaaagaac ctattggttt gttaggatcc tttagtccga ttctatttcg caaaatcgat
      301 ttacataggg tatcaaccgc tttatcttga cctataataa gacctttcaa agcagaatcc
      361 atacgacgta attttgcaaa ttctgcttct tctacctttt gtacgggaac tcctgcaatc
      421 attgaaataa cttctgcaat cttatctgca tcaataacta gtgcattttt gcgaacttga
      481 atttcccaat tctttttact ttcagctaaa gaaatattaa gtgatttctc tttgtcttta
      541 tattcgtttg ccaatttgaa atcttcgtct tttacagctt ttagttttaa tcgattaatt
      601 tctgcaatat tatgctctaa atcttgaata tcttgcggaa taactgcatt gcttatgtga
      661 gctcttgatc ctgcttcgtc cattgcatca atcgccttgt cagggaactg acgatcgtta
      721 atatatctgt cagtaagagt tacacaagct ttaattgcct catcggtata aataacatta
      781 tgatgtgcct cataacgttc ttttatgtta tttaatattt cgatagtctc ttcttttgat
      841 gtaggtttta caataacctt ttgaaaacga cgttccagcg caccatcttt ttcaatactt
      901 tgacgatatt cgtctaaagt cgtagctcca atacattgta tttgacctcg ggccaaagcc
      961 ggcttcatca tattggctgc atcaagcgaa cctgctgttg caccggcacc aataatggtg
     1021 tgtatttcat caataaaaag aataatattg tcgttattct ccaattcttt aagaatggct
     1081 ttaagacgtt cttcaaactg tccgcgatat tttgttccgg caactaacga tgccatatcc
     1141 aatgaaataa ttcgcttgtc cgataaaatc ataggagcac gatgctctac gatgtgattt
     1201 gccaagcctt caacaatggc tgattttcct acacccggat cgcctattaa tacaggattg
     1261 tttttcttgc gtctacttaa aatttgtgct aaacgttcaa tttctttttc acgtccaact
     1321 acaggatcca actttccctg tgctgcggct gccgtaatgt cagaaccaaa agaatctagg
     1381 gcaggggttt tggttgattg tttggtatat gtgccggtat ctgtcgtttt tccattatta
     1441 ctgacagtat ttgccatgta gttgtcatca tcatcttccg aatcattatc gaatttgtca
     1501 ttatcattag aaccggatgc cggcagagtg tcttttttct ctactaaatt acgaatagta
     1561 ttatagctaa ttccttgtgt atgaaacatt tcagacacta agtttgttgt atcttttaaa
     1621 atggctaaca acaaatgtt
//
LOCUS       DAOPID010000254         1592 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_112267_length_1592_cov_3.227066, whole genome shotgun
            sequence.
ACCESSION   DAOPID010000254 DAOPID010000000
VERSION     DAOPID010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112267_length_1592_cov_3.227066"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="PK641_07020"
     CDS             complement(<1..631)
                     /locus_tag="PK641_07020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HPG56128.1"
                     /translation="MNSIILSLIVLGGIGLISALILYYVLAKFKVDENPYIDKVEALL
                     PGANCGGCSFAGCRNFAEKCVSSESMDGLFCPIGKDETMQSIAKLLGKSAQIEEPKIA
                     VVRCQGSCDVRPRTNIYDGVTSCKIANTLYIGESGCPNGCLGYGDCEFSCPFDSIHID
                     LKTNLPVVDEDLCTACGKCVVACPRDIIQIRRKGPKNRRVFVSCINQEKG"
     gene            complement(635..1051)
                     /locus_tag="PK641_07025"
     CDS             complement(635..1051)
                     /locus_tag="PK641_07025"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HPG56129.1"
                     /translation="MASSVTHKGIVTKVSDGMAYVRIEQLSACAECHSSSLCSTAEKQ
                     EKIIETLTDGKKVAVGDLVNIVGKQSQAYTAILIAFVLPMILMLSVLLITKLIALWSE
                     TSCALAALGTLALFYAVLWLFRNNIKAKFTFHIENI"
     gene            complement(1134..>1592)
                     /locus_tag="PK641_07030"
     CDS             complement(1134..>1592)
                     /locus_tag="PK641_07030"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPG56130.1"
                     /translation="SMESTISYRLAIASDLDAVVNIENNCFDTEKFNRRQLSYLMFKA
                     KGCCFVAIDEDKIVGYISLLKHTKRKNLRIYSLAVDKSAQRKHIGQGFIDIAKAYAKE
                     NRLSSITLEVRLDNAAALSLYKRNGFLEEEILPNYFKYNIDAFRMKVSIE"
BASE COUNT          505 a          293 c          244 g          550 t
ORIGIN      
        1 ctcctttttc ttgattgata caagatacaa atacacgtct gtttttagga cctttacgtc
       61 ttatctgaat tatatcgcgt ggacaagcaa ccacacattt accgcacgcg gtacataagt
      121 cttcatcaac aaccggtaaa ttagttttaa ggtctatatg tatgctgtca aaaggacatg
      181 aaaattcaca atctccataa cctaagcatc cgttaggaca tcctgattca ccaatgtata
      241 atgtatttgc aattttacaa gatgtaaccc catcgtatat atttgtacgt ggtctgacat
      301 cacaactacc ttggcaacga actactgcaa ttttaggttc ttcaatttgc gccgatttgc
      361 ctaaaagttt ggctattgac tgcatcgttt catctttacc aatagggcaa aataggccat
      421 ccatactttc tgatgacacg catttttctg caaaatttct acatccggca aaactacatc
      481 caccacagtt tgcaccgggt agtaaagctt caactttatc aatatatgga ttttcgtcta
      541 ctttgaattt ggctaataca taataaagga tgagtgcgga aataagaccg atacctccta
      601 aaacaattaa agataaaatt atgctgttca taacttatat attttcaata tggaacgtaa
      661 atttggcttt tatattgttc cgaaaaagcc ataaaactgc gtaaaataat gcaagggttc
      721 ctaatgcagc taaagcgcat gacgtttcag accataaagc gattaatttt gtgattaaaa
      781 gaactgaaag catcaagatc atcggtaata caaatgcaat taatattgct gtatatgctt
      841 ggctttgttt tcctactatg tttactaaat ctcctacagc aacttttttt ccatcagtaa
      901 gggtttcgat aattttttct tgcttttctg ctgtactgca taatgatgaa gaatggcatt
      961 ctgcacatgc tgataattgc tctattcgaa cataagccat accatcagag actttggtaa
     1021 caataccttt atgtgtaact gaagatgcca tagctggtta aaaaaatcta gtaaatttag
     1081 gctgcaaagt tacaatattt ttttgtttta aatgcgaaaa atatataagg tttctattct
     1141 attgaaactt tcattctaaa agcatcaata ttatatttaa agtaattcgg cagaatttct
     1201 tcctctaaga atccgtttct tttatatagt gataatgcag ctgcgttgtc taatctaact
     1261 tctaatgtta ttgaacttaa tctattttct ttggcgtatg ctttagcaat gtcaataaaa
     1321 ccttgaccaa tgtgtttgcg ttgagcagat ttatcaactg ctaaagaata tattctcaaa
     1381 ttttttcgtt ttgtatgctt aagaagcgaa atatatccga ctattttgtc ttcgtcaata
     1441 gcaacaaaac agcatccttt agctttaaac attagatatg acaattgccg tctgttgaat
     1501 ttttcagtgt caaaacaatt gttttctata tttacaactg catctaaatc tgatgctatt
     1561 gctagacgat acgaaatagt cgattccatc ga
//
LOCUS       DAOPID010000255         1584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_113138_length_1584_cov_3.988882, whole genome shotgun
            sequence.
ACCESSION   DAOPID010000255 DAOPID010000000
VERSION     DAOPID010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113138_length_1584_cov_3.988882"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PK641_07035"
     CDS             complement(<1..403)
                     /locus_tag="PK641_07035"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPG56131.1"
                     /translation="MANRKFITNLFDSIAPAYDNLNHILSLNIDKIWRRRAVQTIRKG
                     RHKYILDVACGTGDFSLALASTGGFVTGIDLSEGMLQIGRKKVAKSKFSSKITMIQGD
                     CEHLPFEDGSFDVVGVAFGVRNFENLRDCICE"
     gene            complement(472..1413)
                     /gene="mdh"
                     /locus_tag="PK641_07040"
     CDS             complement(472..1413)
                     /gene="mdh"
                     /locus_tag="PK641_07040"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HPG56132.1"
                     /translation="MSKVTVIGAGNVGATCANVLAINEVASEVVLIDIKEGLSEGKAM
                     DIMQTAQLIGFDTVVKGVTNDYSQTANSDVVVITSGIPRKPGMTREDLIGVNAGIVKT
                     VAENALKYSPNAIIIMVSNPMDPMAYLTLKSMKLPRNRVIGMGGALDSSRFKYFLSQA
                     LNCNANEVEGLVIGGHGDTTMIPLTRFATYKGIPVSTLMSAEKLEEVAKSTMVGGATL
                     TGLLGTSAWMAPGAAISYLVESIIKDQKKMIVCATSLEGEYGMNDIMIGVPVIIGKNG
                     IEKIVELELNDDEKAKLAASEDAVRKTNSILNDMKLI"
BASE COUNT          489 a          328 c          237 g          530 t
ORIGIN      
        1 tttcacatat acaatcgcgc aaattttcaa aatttctgac tccaaaagct acacccacaa
       61 catcgaaact accatcttca aaaggcaaat gttcgcaatc gccttgtatc attgtgattt
      121 ttgaactgaa ttttgatttt gctactttct ttctacctat ttgtagcatt ccttcagaca
      181 agtctattcc tgtaacaaat ccacctgtcg aagctaaagc caacgaaaaa tctccggtgc
      241 cgcaggcaac atccaaaata tatttgtgtc gacctttgcg aatagtttgc acagctcttc
      301 ttctccatat cttatcaata tttaaagata aaatatgatt tagattatcg taagcaggag
      361 ctatagaatc aaaaagattt gtaataaatt ttcggttggc cataataaaa aaaatggtag
      421 agatgcagat attattcaca catctctacc gtttaatttg caaaaatcac attagattaa
      481 tttcatgtcg tttaggatac tgttagtctt acgaacagcg tcttcgctgg cagctaattt
      541 agctttctcg tcatcgttta attctaattc aacgattttc tctataccat tttttcctat
      601 gattactgga acaccaatca tgatgtcgtt cataccatat tcaccttcaa gtgaagttgc
      661 acaaacaatc atcttctttt ggtcttttat aattgattca actaagtaag aaattgcagc
      721 acccggagcc atccatgcag aagtgcctag taagccggta agagttgcac caccaaccat
      781 tgttgatttt gctacttctt ccaatttttc agcagacatt aaagtgctaa caggaatacc
      841 tttatatgtt gcaaaacgag ttaaaggaat catagttgta tcaccatgac caccaattac
      901 taaaccttca acttcattag cattgcagtt taatgcttgg ctcaagaaat atttgaaacg
      961 gctgctatcc aatgcaccac ccatacctat aacccggtta cgaggtaatt tcattgattt
     1021 taatgtgagg tatgccatag ggtccatagg gtttgaaacc ataataatta tagcatttgg
     1081 agaatatttc aaagcgtttt ctgctactgt ttttactatt ccggcattaa cgcctataag
     1141 atcttcacga gtcatacccg gtttacgtgg aattcctgat gtaattacaa ccacatcaga
     1201 attggctgtt tgagaataat cgtttgttac acccttaaca acagtgtcga atccaattag
     1261 ttgagcagtt tgcatgatat ccatcgcttt tccttcggat aagccttctt ttatatcaat
     1321 caatacaact tcgctcgcta cttcgttgat cgctaataca ttagcacatg tagcacctac
     1381 attacctgca cctatgaccg ttacttttga catgatttat ttatttaaat ggttaattga
     1441 ttcttaattg cccctgcaaa aatacgcttt tttatcaaaa agtagtacat ttgcaagtct
     1501 aatttcagat gagaaacaga attatttata tatccgcttg tattgccatc acaatactat
     1561 ttactcagtg ttcagtaaaa aaga
//
LOCUS       DAOPID010000256         1578 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_113806_length_1578_cov_2.775443, whole genome shotgun
            sequence.
ACCESSION   DAOPID010000256 DAOPID010000000
VERSION     DAOPID010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113806_length_1578_cov_2.775443"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="PK641_07045"
     CDS             <1..670
                     /locus_tag="PK641_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPG56133.1"
                     /translation="GSYIHVGPEIGVASTKAFTGQITVLSMLALTIAKLRGTISNEHY
                     VEIIQELQDIPNKLQEVLKLDDKIRDLSKIFTYAHNFIYLGRGYNYPAALEGALKLKE
                     ISYIHAEGYPAAEMKHGPIALIDNEMPVVALATSDRIYEKTVSNIQEIKARKGKVIAV
                     VTEGDEVVSSIVDYCIEIPKTLEMFAPIVSAIPLQLFAYYIAVNKGRDVDQPRNLAKS
                     VTVE"
     gene            676..>1578
                     /locus_tag="PK641_07050"
     CDS             676..>1578
                     /locus_tag="PK641_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005837255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPG56134.1"
                     /translation="METLKHECGVAMVRLRKPLQFYEQKYGSWMYGLNKLYLLMEKQH
                     NRGQEGAGMACVKLDMQAGEEFMFRERAEGSGSISEIFNTAHTPINNIPSEKLHDAEY
                     AKSAIPFAGELYMGHLRYSTTGKSGLSYVHPFMRRDNYRSRNLTICGNFNLTNVDEVF
                     DSISIMGQHPRQYADTFIILEQLGHRLDREVERLYSKAKKSKKSPTCMSKYIEQNICI
                     ENVLSSAVTMWDGGYVICGLTGSGESFTVRDPWGIRPAFYYADEELVVVASERPVIQT
                     ALNLSVDKICELEPGQALIIGNNGS"
BASE COUNT          523 a          224 c          349 g          482 t
ORIGIN      
        1 cggatcttat atacatgtcg gtcctgagat aggtgttgca tcaacaaaag catttaccgg
       61 acaaataaca gtattatcga tgttagcact tactatagct aaattacgtg gaactattag
      121 taatgagcat tacgtcgaaa ttattcaaga attgcaagat attcctaata aactgcagga
      181 ggtcttgaaa cttgacgata aaatacgtga tttgtcaaaa atctttacct acgctcacaa
      241 ctttatttat ttaggcagag gatataatta tccggcagct ttggaaggag ctttaaagct
      301 taaagaaatt tcttatatac atgccgaagg ctatcctgct gcagaaatga aacatggccc
      361 aattgcttta atagacaacg aaatgcctgt agttgcattg gctacatcgg atagaattta
      421 tgaaaaaacg gttagtaata ttcaagagat aaaagcaaga aaaggtaagg ttatagcagt
      481 tgttactgaa ggcgatgagg tggtttcttc tattgtagat tattgtatag aaataccaaa
      541 aacattagaa atgtttgcac ctatagtatc ggctatacca ctacaattat ttgcttatta
      601 tatagcagtt aacaaaggta gagatgttga tcaacctcgt aacttggcaa aatcggttac
      661 ggtagaataa ttaatatgga aactctaaag catgaatgtg gtgtcgcaat ggttcgttta
      721 agaaaacctt tacagtttta cgaacaaaaa tacggttcat ggatgtatgg tttgaataaa
      781 ctatacttgc ttatggagaa acagcataat agagggcaag agggtgcagg aatggcctgt
      841 gtaaaacttg atatgcaagc gggtgaggag tttatgttta gagaaagggc agaaggctcc
      901 ggttcaatta gtgagatatt taatactgct catacaccaa ttaataatat tccatcagaa
      961 aagcttcatg atgcagaata tgctaagtct gcaattcctt ttgcaggtga attatatatg
     1021 gggcatctaa ggtatagtac taccggcaaa tcgggtttat cttatgtaca tccatttatg
     1081 cggcgagata attatcgctc aaggaatctt acaatatgtg gtaattttaa tttgactaat
     1141 gttgatgagg tttttgattc tataagcatt atgggacaac atcctcgtca gtatgctgat
     1201 acatttatta tattggagca attggggcat cgcttggata gagaggttga gagattatac
     1261 tcaaaggcaa agaagagcaa gaaatctcca acatgtatgt ctaaatatat tgagcaaaat
     1321 atatgtattg aaaatgtact gagtagtgct gttactatgt gggacggagg ctatgttata
     1381 tgcggtttga caggcagtgg tgagagtttt acggtgagag atccatgggg aattagacct
     1441 gctttttatt atgctgatga agagttagta gtagtagcat ctgagcgacc ggttattcaa
     1501 acagccttaa atctttctgt agataagatt tgtgagttag aaccgggaca agctttgatt
     1561 atagggaata atggtagt
//
LOCUS       DAOPID010000257         1570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_114721_length_1570_cov_3.328053, whole genome shotgun
            sequence.
ACCESSION   DAOPID010000257 DAOPID010000000
VERSION     DAOPID010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114721_length_1570_cov_3.328053"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /gene="uppS"
                     /locus_tag="PK641_07055"
     CDS             <1..562
                     /gene="uppS"
                     /locus_tag="PK641_07055"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HPG56135.1"
                     /translation="LYVFSTENWNRPEDEVSALMKLLVSTIESETVNLNKNNVRLKTI
                     GNTDRLIKQVNNKLQEGIELTSKNTGLTLVLALSYSARWEITNAVKQIALNVTTQGID
                     PNTIDETTVASFLTTKEIPDPELLIRTSGEYRISNFLLWQIAYTELYFTPVLWPDFNK
                     EDFYGAIIDFQQRERRFGKTSEQVAK"
     gene            566..>1570
                     /locus_tag="PK641_07060"
     CDS             566..>1570
                     /locus_tag="PK641_07060"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HPG56136.1"
                     /translation="MLHKYISILVILTIVGTLSAYADILSTDSINNQQNNVFFSSSDS
                     VNVLVGENLPELDYANPREYEIGEITISGKNNYEDYVLIGFSGLKVGEKILIPSDAIT
                     NVIRRFWKQGLFSNIQVEIVSIRENKIWLNIQLDQRPRISNINIIGAKKKEKEDIEKG
                     IGMSKGTQITPYIIDKAKKFIERYYIEKGYYYTKVSIVPREDDAEGNNNIVDIRINKG
                     SKITVNRLIIDGNASISKRKLDGAMKKTNDRHNILNIFRSKKFLPKEYENDKNLFIEK
                     YNEYGFRDAEIVCDSVFPDSIKPTKYVNVYIKVNEGKRYYFRDINWMGNTIYTTDYLR
                     R"
BASE COUNT          606 a          251 c          266 g          447 t
ORIGIN      
        1 tttgtacgta ttttcaactg aaaattggaa tagacctgaa gacgaagtta gcgcattgat
       61 gaagttacta gtttcaacta ttgaatccga aactgtaaat ttaaacaaaa acaatgtacg
      121 cttaaaaacc atcggaaata cagatagact tataaaacaa gttaacaaca agttacaaga
      181 aggcatagag ttaacatcaa aaaatacagg attaacttta gtattggcac tcagttattc
      241 tgctagatgg gaaatcacta atgctgtaaa acaaatcgca ttaaacgtaa ctacacaagg
      301 tattgatcct aatacaattg atgaaacaac tgtagcatct tttctcacaa ctaaagaaat
      361 acccgatcct gaattactaa tcagaacaag cggtgaatat cgcataagta attttttatt
      421 gtggcaaata gcatacaccg aattatattt tacccctgta ttatggcctg attttaataa
      481 ggaggatttt tatggtgcca ttattgattt tcaacaacgt gaacgtcgtt ttggaaagac
      541 gagcgaacaa gtagccaaat aatttatgtt gcacaaatat atatctatcc ttgttatttt
      601 gacgattgtt ggaacattgt ctgcatatgc agatatatta tcgaccgaca gtattaacaa
      661 tcagcaaaac aatgtttttt tctcgtcatc cgacagcgtc aatgttttgg ttggagaaaa
      721 tctacccgag ctcgactacg caaatccacg tgaatacgaa atcggagaga ttaccatatc
      781 cggaaaaaat aattatgaag attacgtact tataggattt tccggtttaa aagttggaga
      841 aaaaatatta attcccagtg atgccatcac taatgttatt agacgtttct ggaaacaagg
      901 attattctca aatattcaag tagagattgt ctcgattaga gagaataaaa tatggctaaa
      961 catacaatta gaccaacgtc cacgcatttc aaacataaat attatcggtg ctaaaaagaa
     1021 agaaaaagaa gatattgaaa aaggcatcgg catgtcgaaa ggcactcaaa taacaccata
     1081 cataattgac aaagcgaaaa aatttattga acgctactat attgaaaaag ggtactatta
     1141 cacgaaggta tctatcgttc cacgcgaaga cgatgcagaa ggcaataata acattgttga
     1201 tataagaatc aataagggct caaaaattac tgttaataga ttaattattg atggcaatgc
     1261 gtctatctct aaacgaaaat tagacggagc catgaaaaag actaatgata gacataacat
     1321 attgaacata ttccgcagta agaaatttct acctaaagaa tacgaaaacg ataaaaacct
     1381 atttattgaa aaatataatg aatacggctt tagagatgct gaaattgttt gtgatagtgt
     1441 attccccgat tctattaagc ctacaaaata cgtaaatgtt tacattaagg taaacgaagg
     1501 taaacgatat tatttcagag acatcaattg gatgggtaat accatttaca caaccgatta
     1561 tttacgcaga
//
LOCUS       DAOPID010000258         1551 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_116854_length_1551_cov_3.891043, whole genome shotgun
            sequence.
ACCESSION   DAOPID010000258 DAOPID010000000
VERSION     DAOPID010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116854_length_1551_cov_3.891043"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="PK641_07065"
     CDS             complement(<1..522)
                     /locus_tag="PK641_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG56137.1"
                     /translation="MKNRIKLLLVSMFFVVACFATIYEKLWNEVEVYEQDGLPKSAKK
                     TTDSIYVIAQKEKNYPQLIRSMVYQLKEDMSIDEDSFFVHLNQFKIFAKDCPDKVASS
                     VAYSIMAELMQSYYQANSFKIDDRNQIAVNLDDITEWTSEMYKNVIVDWTKKSIEAEK
                     ALASTDIDMYRPII"
     gene            complement(519..>1551)
                     /locus_tag="PK641_07070"
     CDS             complement(519..>1551)
                     /locus_tag="PK641_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPG56138.1"
                     /translation="KDLLNHRLITLSSGQLRRLLITKALLEKPRMLIFDNPFIGLDVE
                     TRELMNIVFEKLMKNGVQILFLIPVLRDIPACTTHVLYLDNCSPIFQGKYSDFKNVVP
                     SESVVIEKKFDFSKLSLLMHSEYQNVVKMEELDINYETIVYQKGINWTIKKGEKWALS
                     GPNGSGKSTLLSYIFADNPQAYGKPLYLFDKKRGSGESIWDIKKRIGFTSSEMHLYYL
                     ENVPCISVVESGFFDSIGLFRKCSPSQAALAESLMDYLGLSELKNVSFLNVSSGEQRL
                     LLFARALVKNPELLILDEPFHGLDYGKKQLCLSMINDYAIQAEKTLIFVTHYKEEIPA
                     CVTNIKRLS"
BASE COUNT          513 a          288 c          196 g          554 t
ORIGIN      
        1 tataatcggt ctatacatgt caatatcggt tgatgccaat gctttttcag cttcaattga
       61 tttctttgtc caatcaacaa ttacgttctt gtacatttca gaagtccact cggtaatatc
      121 atccaaatta acagcaatct gatttctgtc atctatttta aaactatttg cttggtaata
      181 tgattgcatc aactcagcca taattgaata tgcaacagat gatgcaactt tatccggaca
      241 atctttcgca aagattttaa attgatttaa atggacaaaa aatgaatctt catcaatact
      301 catatcctct ttcaattgat ataccatact tctaatcaat tgaggataat tcttctcttt
      361 ttgagcgatt acgtaaattg aatctgtagt ttttttagca gacttgggca aaccatcttg
      421 ctcataaact tctacttcat tccaaagctt ttcataaata gttgcaaagc aagcaactac
      481 aaaaaacatc gatactaata ataatttaat tctgtttttc atgatagtct ttttatattg
      541 gtaacgcagg cgggtatttc ttccttataa tgagtaacaa agattagtgt tttttctgct
      601 tgtatagcat aatcattaat catacttaag cataattgtt ttttgccata atcaagaccg
      661 tgaaaaggtt catctaagat taataattcc ggatttttaa ctaatgctcg ggcaaataat
      721 aataatcgtt gttcgcctga cgatacattt aaaaatgaaa cgttttttag ttcacttaaa
      781 cctaaataat ccattaatga ctctgccaat gccgcttgtg atggcgaaca tttgcgaaat
      841 aatcctatac tatcaaagaa acctgattca actacagata tacaaggtac attttctaaa
      901 taatataaat gcatctctga agatgtaaat cctatacgtt tttttatatc ccaaatactc
      961 tcaccggatc ctcttttttt atcaaataaa tataatggtt tcccgtatgc ctgaggatta
     1021 tccgcaaaaa tataactcaa tagtgtggat tttcctgaac cattaggacc tgacaatgcc
     1081 catttttctc ctttttttat tgtccaattt ataccttttt gatacactat agtttcataa
     1141 ttaatatcca attcttccat ctttaccaca ttctgatatt ctgaatgcat cagtaatgac
     1201 aacttagaaa aatcaaattt tttttcaata actactgatt ctgatggtac tacattctta
     1261 aaatctgaat atttaccttg aaaaatcgga ctgcaattat ccaaatataa tacatgtgtt
     1321 gtacatgccg gtatatctcg caaaacaggt atcaaaaaca atatttgaac accatttttc
     1381 atcaatttct caaagactat attcatcaac tcacgagttt caacatctaa gcctataaac
     1441 ggattatcaa atatcagcat tctaggtttt tccaataaag cctttgttat taacagtctg
     1501 cgtaattgcc cgctagacaa agttataaga cggtgattca gaagatcttt a
//
LOCUS       DAOPID010000259         1548 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_117191_length_1548_cov_3.808439, whole genome shotgun
            sequence.
ACCESSION   DAOPID010000259 DAOPID010000000
VERSION     DAOPID010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117191_length_1548_cov_3.808439"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1526)
                     /locus_tag="PK641_07075"
     CDS             complement(<1..1526)
                     /locus_tag="PK641_07075"
                     /inference="COORDINATES: protein motif:HMM:NF020926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HPG56139.1"
                     /translation="MALSLYLKINPDLYSANNAKTFINEKGYLGFIDWLKSEPIELSD
                     LLRNSISITNKQLKNKYINSFEWLEDFIGEQGTFSKIIKEFEQNVEYLKKEYEKAMRA
                     RDEKTASVFSHKLERYQKNDLIDFLVRGNILPKYGFPIDSVELTQNIASQSFKSLNLS
                     RDLSVAIAEYAPSSEVVADGGLYTSRYIRKPIVNKSEMSEFETAYLATCTNPKCGNIN
                     YSKMPIGSDGVSCAICGKELKNRDFYKSIEPRAGFIAEEVIKDVPLSSQERKYKTEAI
                     YIGDKDAYPISKYEYEFENIKIEVESTANDSLVVKSTDFFYVCPKCGYSIASDETNKL
                     KEYDYKGIGCGHIETNKDIHNNPFGKGKCVNNSLTRYSLHHEFKTDVAKISFGCDTSD
                     YPTMLSVMYALLNSFAHELNIERRDIKACLTYKISNGRTENKIIIYDAVPGGAGHSRR
                     LVTENGEVLKAVIKRAINLLDSCVCFPSCYRCLRSYENQKKHEILDRENALNFLKQLG
                     "
BASE COUNT          478 a          283 c          211 g          576 t
ORIGIN      
        1 tatcctaatt gctttaaaaa attcagtgca ttttctctgt caagaatttc atgttttttt
       61 tggttttcat agctacgtaa acaacgatag caagaaggaa agcatacaca agaatccaat
      121 aaatttattg ctctttttat taccgctttc aatacttctc cattttctgt tacaagacgt
      181 cttgaatgtc ctgcgccacc cggaactgca tcgtaaatta ttatcttatt ctctgttctt
      241 ccgtttgaaa tcttataagt taaacaagcc ttgatatctc gtctttcaat gttaagttca
      301 tgtgcaaaag aattaagcag agcatacata accgacaaca ttgttggata gtctgaagtg
      361 tcacaaccaa aagatatctt tgccacatct gtcttaaatt catgatgcaa actatatctt
      421 gtgagtgaat tatttacaca ttttcctttc ccgaaaggat tattatgtat gtctttattg
      481 gtttcaatat gcccacatcc aattccttta taatcatatt cttttaattt attagtctca
      541 tcgcttgcaa ttgaatatcc acattttgga cacacataga agaaatcagt agatttaaca
      601 accagagaat cattcgcagt tgattcaact tctatcttga tattttcaaa ctcatattca
      661 tatttactaa tgggataggc atctttatca ccaatataga ttgcttctgt tttatatttt
      721 ctttcttgtg aacttagcgg aacatcttta attacctctt ctgcaataaa acctgctctt
      781 ggttcaatac ttttatagaa atctctattt tttagttctt taccacaaat tgcgcaagat
      841 actccatctg aacctattgg catcttagaa taatttatat taccgcattt aggattagta
      901 caagtagcaa gataagctgt ttcaaactcg ctcatttcgc ttttgtttac aataggtttt
      961 ctaatgtaac ggcttgtata taagccacca tcggcaacta cttctgaaga aggtgcatat
     1021 tcggcaatag caactgataa atcacgactt aagtttaaag atttgaaact ttgagatgca
     1081 atgttttgtg ttaattctac tgaatctata gggaatccat atttgggaag aatattacct
     1141 cgaacaagaa aatcaatcaa atcgtttttt tgataccttt ctaatttatg actaaataca
     1201 gaagcagttt tttcatctct tgctcgcatt gccttttcgt attccttttt taaatactca
     1261 acgttctgtt caaactcctt aataattttt gaaaatgttc cctgctctcc aataaaatct
     1321 tccaaccatt caaaactgtt gatgtatttg tttttcagtt gtttatttgt gattgaaata
     1381 gaatttctta ataaatcgga taattcaatt ggttcagact taagccaatc aataaaaccc
     1441 agatatccct tttcgttaat aaatgttttc gcattattag cggaatacaa atcaggattg
     1501 atttttaagt ataagcttag agccacggca taaatatgac gttttaaa
//
LOCUS       DAOPID010000260         1514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_121246_length_1514_cov_4.244688, whole genome shotgun
            sequence.
ACCESSION   DAOPID010000260 DAOPID010000000
VERSION     DAOPID010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121246_length_1514_cov_4.244688"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="PK641_07080"
     CDS             <1..532
                     /locus_tag="PK641_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility lipoprotein GldJ"
                     /protein_id="HPG56140.1"
                     /translation="LYNMAGNVNEWTMDVYRQTTTDEVQEYNPFRGNVYMAPVKDSLN
                     KFELDSLGRLKYAVEYSKEDSMAAYAKLDVRNYNDGDARSSYSRDKWKEVIDPDLATK
                     RLYDADEPTDPESLLTTKISNTSRVYKGGSWKDRSYWLSPATRRYMEQSKRSNDIGFR
                     CAMNRVGSDKGNNSKK"
     gene            603..>1514
                     /locus_tag="PK641_07085"
     CDS             603..>1514
                     /locus_tag="PK641_07085"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPG56141.1"
                     /translation="MNIETTTLSNGIRLIHVRCKGDVAYCGVMVNVGTRDELESENGM
                     AHLTEHILFKGTEKRNYIQIINRIEDVGGEMNAYTTKEETTIYALFLKRYLERAMELI
                     ADMIFNYKFDENLLQKEKEVVADEIDSYTDNPSELILDDFEDELFAGHSLGRNTLGSK
                     KILSKITVDALKSFIGRCYTTDEMVFYSMGEFSFERVEKLAQKMFGIYPSTQRMFKRT
                     APINYEPKTLTKHKATSQVHLTFGNRAYNAFDDKRYSFFLLNNILGGGNMNSRLNMAL
                     REKHALVYSVESMYTPYSDVGNWNVYLG"
BASE COUNT          521 a          217 c          308 g          468 t
ORIGIN      
        1 tctgtataat atggccggta acgttaacga gtggacaatg gatgtttatc gtcaaacaac
       61 tacagatgag gttcaagaat acaatccatt ccgtggtaac gtatacatgg ctcctgtaaa
      121 agattctttg aacaaatttg aattagatag tcttggtcgt cttaaatatg ctgttgaata
      181 ttcgaaagaa gatagcatgg cagcgtatgc taaattagat gtacgtaact acaatgatgg
      241 tgatgcacga agcagctata gccgtgacaa atggaaagaa gttattgatc ctgatttagc
      301 tacaaaacgt ttgtatgatg ctgacgaacc cactgatcct gaaagtctgt tgacaactaa
      361 gatttcaaat acatctcgtg tttataaagg tggtagctgg aaagatcgtt catactggtt
      421 aagccctgca acacgtcgtt atatggaaca atcaaaacgt tctaacgata taggtttccg
      481 ttgtgccatg aatcgtgtag gttctgataa gggtaataat tctaaaaagt aatctgaaat
      541 aataagatat taaaaggata gcttccggct atcctttttt ttatatatta agagtctgtt
      601 ttatgaatat agaaactaca acattatcga atggaattag gcttattcat gtgcgctgta
      661 agggtgatgt agcttattgc ggagtgatgg taaatgtagg aacacgtgat gagttggaat
      721 ccgaaaatgg tatggcacat ctaacagaac atattctatt taagggtact gaaaaaagaa
      781 attatattca gataataaat agaattgaag atgttggagg cgaaatgaat gcctatacaa
      841 caaaagaaga gacaacaata tatgcactct ttttaaaacg ttatcttgaa cgtgcaatgg
      901 agcttattgc agatatgatt ttcaattata aatttgatga gaatttattg caaaaagaaa
      961 aagaggttgt tgcagatgaa atagattcat atacagataa tccatctgaa cttatattag
     1021 acgattttga agatgaacta tttgcaggac attccttagg aaggaataca ttaggctcta
     1081 agaaaatact gtcaaaaatt actgtagatg ctctaaaatc ttttatcggc agatgttata
     1141 ctaccgacga aatggtattt tacagtatgg gtgagtttag ttttgaaagg gttgagaagt
     1201 tggctcaaaa aatgtttgga atatatccaa gtacacaacg tatgtttaag cgcaccgctc
     1261 cgataaatta tgaacctaaa acactcacaa agcataaagc gacttcacaa gttcatttga
     1321 cttttggcaa cagagcatat aacgcttttg atgataaacg ttattcgttt tttttactaa
     1381 acaatattct tggaggaggt aatatgaata gtcgtttaaa catggcttta cgcgaaaaac
     1441 atgccttggt ttattcggtt gaatctatgt acacaccata ttctgatgtg ggtaattgga
     1501 atgtatatct tggc
//
LOCUS       DAOPID010000261         1463 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_127808_length_1463_cov_2.909091, whole genome shotgun
            sequence.
ACCESSION   DAOPID010000261 DAOPID010000000
VERSION     DAOPID010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127808_length_1463_cov_2.909091"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..1058
                     /locus_tag="PK641_07090"
     CDS             75..1058
                     /locus_tag="PK641_07090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPG56142.1"
                     /translation="MKLSVIIVNYKVQDYLLQCLDSLSKSLSEIVAEVFVVDNASNDE
                     SQKLVKNYFPETIFISNAENVGFAKANNQAMQMAKGDYVLLLNPDVIIGENCIKHVCD
                     FADTASDFGALGVRMINSNGNFLPESKRNIPSLWAAFCKFSGLSKLFKHSRFFNMYYN
                     TILTDDQTGESQILAGAFMLLNKKTLGKEMLFDEQFFMYGEDIDLSYRILRSGLKNYY
                     YPETILHYKGESSKSDVKKYISSFFDAMILYYKKHSKSRYAYYSVNMAANMLKMFAYF
                     TKKIRQSPKDNREIITYSSSDYSYSDLIQKIESNKGVNRICIYHPNFSITI"
     gene            1055..>1463
                     /locus_tag="PK641_07095"
     CDS             1055..>1463
                     /locus_tag="PK641_07095"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPG56143.1"
                     /translation="MKLLDNEILKLRALEPEDIDMFSLWENDSAYWKQGQTIAPYSRY
                     ALKQYIAAAQSDIYETKELKLVIVRICDNQAIGIVDLFNIDHYHSRLEFGILIDPKYK
                     RKGYAIQTIEIVSKYVLDFLHIHQIYCRVSENNE"
BASE COUNT          522 a          206 c          261 g          474 t
ORIGIN      
        1 atttatgata ctcaatacat aaaaccgaaa cctaaaaatt taattattcg ttttagcgag
       61 tggttggcaa aaagatgaaa ctatcagtta ttatagttaa ttataaagta caagattatc
      121 tattacaatg tttggattct ctttctaagt ctctgtcaga aattgtcgca gaggtttttg
      181 ttgtagataa tgcctcaaat gatgaatctc agaaacttgt aaaaaactat ttcccagaaa
      241 ctatatttat aagtaatgcc gaaaatgtag gatttgccaa agctaataat caagctatgc
      301 aaatggctaa aggtgattat gtgttattat tgaatccgga tgtgattatt ggagaaaact
      361 gcattaagca tgtatgtgat tttgcagaca ctgcatccga ttttggagcc ttaggagtga
      421 gaatgataaa ttcgaacgga aattttttgc ctgaatctaa aaggaacata ccatctttat
      481 gggctgcatt ttgtaagttt tcaggtttat ctaagttgtt taagcattca agatttttca
      541 atatgtatta taatacaatc ttgactgatg accaaaccgg cgaatctcag atccttgccg
      601 gagcatttat gttgctaaat aagaaaacat taggtaaaga aatgttattt gacgaacaat
      661 tttttatgta tggcgaagac attgacttat cgtacagaat attgcgatca ggcttaaaaa
      721 attactatta tcccgaaacc atattgcatt ataaaggaga atcttccaag agtgatgtaa
      781 aaaaatatat atcatcattc ttcgatgcga tgatattgta ttataaaaaa cattctaaaa
      841 gtagatatgc ttattatagt gtgaatatgg ctgctaacat gctcaaaatg tttgcttatt
      901 ttaccaaaaa gatacgtcaa agtcctaaag ataataggga aataataaca tactcaagct
      961 cagactattc gtattctgat ttgatacaaa aaatagaaag taacaagggt gtaaatcgta
     1021 tttgcattta tcatccgaat ttcagcataa caatatgaaa ctactggata acgaaatatt
     1081 gaagcttcgt gcgttggaac cagaagatat agatatgttt agtctttggg aaaatgatag
     1141 tgcctattgg aaacaaggtc aaactatagc tccgtattcg cgttatgctt taaaacagta
     1201 tattgctgca gctcagtctg atatatacga gactaaggag cttaaattag taatagtaag
     1261 aatttgtgat aatcaagcta ttggcatagt cgatcttttt aatatcgatc attatcatag
     1321 tcgtttggag tttgggatac taatagaccc gaagtacaaa cgaaaaggct atgctataca
     1381 aactatcgaa attgtaagta aatacgtgtt agacttcttg cacattcatc aaatatattg
     1441 tagagtctcc gaaaataatg agg
//
LOCUS       DAOPID010000262         1457 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_128583_length_1457_cov_3.655492, whole genome shotgun
            sequence.
ACCESSION   DAOPID010000262 DAOPID010000000
VERSION     DAOPID010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128583_length_1457_cov_3.655492"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PK641_07100"
     CDS             complement(<1..187)
                     /locus_tag="PK641_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG56144.1"
                     /translation="MKKIFICLFAVIFYISSASASSESQAFAMAKNLDIFNSVFKELN
                     LHYVDSIPIEKIVSAGIN"
     gene            complement(348..421)
                     /locus_tag="PK641_07105"
     tRNA            complement(348..421)
                     /locus_tag="PK641_07105"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Asn,seq:gtt)
     gene            complement(529..957)
                     /locus_tag="PK641_07110"
     CDS             complement(529..957)
                     /locus_tag="PK641_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HPG56145.1"
                     /translation="MLDKILSISGKPGLYKLISGTKKMIIVESLADKKRMPAYSNEKV
                     ISLNDIAIYTDEEEVPLRTVFETIKTKEDGKEIKLDAKTASPEELRNYLTSVLPNFDK
                     ERVHNSDIKKLILWYNILTNSGENDFSEPKKDDDDEKAEA"
     gene            complement(980..>1457)
                     /locus_tag="PK641_07115"
     CDS             complement(980..>1457)
                     /locus_tag="PK641_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HPG56146.1"
                     /translation="MKQSEIHGMSQRGGDVQSNLRISDSPIASDLIPLKGADLILSLE
                     PMEALRYIPFLSDNGWIVSSSLAFKNIPDYPDEESVLNEIRNIKNSCLLDVEAIAKEV
                     GSVRTSNMVLLGAALPYLGIPELYIENGIKSIFAKKGEEIVQLNLKALRAGYNHKK"
BASE COUNT          450 a          240 c          223 g          544 t
ORIGIN      
        1 catttattcc ggcagataca attttttcga taggtataga atctacataa tgcaaattca
       61 attctttaaa tacggagtta aaaatatcta gattttttgc cattgcaaag gcctgagatt
      121 cagaagatgc tgatgccgat gatatataaa aaattacagc gaacagacag ataaaaattt
      181 ttttcataga aaatattatg ctgatatagt ttgtttatag tattatcata caaaaatagc
      241 tttttttgtg gtacttacta tttttcttta taaactttaa tagcttggag tagtatttta
      301 ttaagaacat aaaaaaagca tccaactaaa agtcagatgc ttttttgctc tccctcttgg
      361 acttgaacca aggaccctct gattaacagt cagatgctct aaccgactga gctaaggaag
      421 agtgttattg cttctcaaaa gcgatgcaaa agtaatgctt atttggctat tgtgcaagtt
      481 ttttgtcaaa atttttgata tttttaattc aaatgcacac tttagagttt aagcctctgc
      541 tttttcgtcg tcgtcgtctt ttttaggttc agaaaagtcg ttttcgccgc tattggtcaa
      601 gatattatac cacaatataa gtttcttaat atcactatta tgtacacgtt ctttatcaaa
      661 attaggtaat acgcttgtta aatagttgcg taattcttca ggagatgctg ttttggcatc
      721 taatttaatt tctttaccgt cttcttttgt tttaattgtt tcaaaaactg tgcgcaatgg
      781 aacttcttct tcgtctgtat agatagctat atcatttaat gatataacct tttcgtttga
      841 atatgccggc attctttttt tatctgcgag tgattctact ataatcattt ttttagttcc
      901 agatattaat ttatacaaac ccggttttcc tgaaatggat aaaattttgt ctaacataat
      961 gtttaaatta ttaattgtat tattttttgt gattatatcc ggctctcaaa gcctttaaat
     1021 ttaattgaac aatttcttcg ccctttttag caaaaataga tttgattcca ttttcaatgt
     1081 ataactcagg tatacctagg taaggtaggg cagcacctaa taataccatg ttggatgtac
     1141 gcactgagcc tacttctttt gcaatagctt ctacatcgag taaacaagaa ttcttgatat
     1201 ttcttatttc atttaaaaca gactcttcat cgggataatc aggtatgttt ttaaatgcta
     1261 acgaactact tacaatccat ccattatcag ataagaatgg aatataacga agtgcttcca
     1321 tgggttcaag tgacaatatt aaatcagctc cttttaatgg aatcaaatca gaagcaatag
     1381 gactgtcaga gatacgtaaa tttgattgta catctccacc acgctggctc ataccgtgta
     1441 tctcagattg cttcata
//
LOCUS       DAOPID010000263         1420 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_133771_length_1420_cov_3.076923, whole genome shotgun
            sequence.
ACCESSION   DAOPID010000263 DAOPID010000000
VERSION     DAOPID010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133771_length_1420_cov_3.076923"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="PK641_07120"
     CDS             complement(<1..802)
                     /locus_tag="PK641_07120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HPG56147.1"
                     /translation="MNGQDYTFSNHNIVPFSLNPALAGNANAIRIATNYRQQWMNLGN
                     RYHTARISYDQNFYKQMCSLGFAYTYDNMSNGIFQTNEFALVYSHNIRLSDGYFIRLG
                     IQGSLFLNYFGYGNLIFEDQYDPNSGSINPTTLESFENDNRIIGDFSAGIAFVMENKL
                     TIGFSANHLSEPDNGFIDKNRNKIYRKYVAHINYVYDFKHQSGLYKKRDLSSTYLFIN
                     ANYQHQYNFNAAYLGAGVAFKPLILGIAYKSNFIDSNIASAMVGVHYKG"
     gene            complement(862..>1420)
                     /locus_tag="PK641_07125"
     CDS             complement(862..>1420)
                     /locus_tag="PK641_07125"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPG56148.1"
                     /translation="SVLLVPQARDSIHFEVYPTYLEEGNSQVRLTGITPTNTTWNWNM
                     DDNSDILVGNNITYNYNIAGRDSFLINIVAVDAYGCVYTGEEAVYTWRDFWAPNAFTP
                     DGNELNDSFGFKAREYFTKFEFIIYNRLGEIVYSGKSTADRWDGNYKGVPCPWGIYGW
                     VVNYTSDYKGVVKSGTEKGYVTIVR"
BASE COUNT          444 a          262 c          232 g          482 t
ORIGIN      
        1 gacctttata atgtacgcca accatggcag atgctatatt agagtctata aagttactct
       61 tataggcgat acctaatatt aaaggtttaa atgcaactcc tgcacccaaa tatgctgcat
      121 taaaattata ttggtgttga tagtttgcat tgatgaataa gtatgtagat gacagatctc
      181 tttttttata caagccgctc tgatgtttaa agtcgtacac ataatttatg tgggcaacat
      241 attttctata aattttattc ctattcttat cgataaagcc attgtccggt tcagataagt
      301 ggtttgctga aaatcctatt gtcagtttat tctccataac aaaggcaata ccggcagaaa
      361 agtctccaat aattcgatta tcattttcaa aactttcaag tgtagtaggg ttgatgcttc
      421 ctgagttggg atcgtattgg tcttcaaata taagattgcc gtatccaaaa taatttaaga
      481 acagagaacc ctgtatacct aaacgaataa aatatccatc tgataaacgt atattatgag
      541 agtataccag cgcaaattcg ttagtttgaa atattccgtt agacatatta tcgtaagtgt
      601 aggcaaaacc aagagagcac atctgcttat aaaaattttg gtcgtatgat atacgtgcgg
      661 tatgatatct attacctaaa ttcatccatt gttggcgata attagtagca atacgtatgg
      721 cgtttgcatt acctgccaat gcaggattta aactaaatgg gactatgtta tgattgctga
      781 aggtatagtc ttgtccgttc acttttacac aaaaaagcaa gattaaaatg atagtgcatg
      841 acaagaactt cttcatgtta tttatctaac aattgtaaca tatccttttt cagttcctga
      901 tttgacaaca cccttatagt cgcttgtata attaacaacc catccatata taccccatgg
      961 acaaggaact cctttataat ttccatccca acgatcggct gtactttttc cggagtatac
     1021 aatttctcct aaacggttat aaataatgaa ttcgaatttt gtaaaatact ctcgtgcttt
     1081 aaatccaaaa ctgtcattca gttcgtttcc atcaggtgta aatgcattcg gtgcccagaa
     1141 atctcgccat gtatatacag cttcttctcc cgtgtataca catccgtaag catctaccgc
     1201 aacaatatta attaagaatg aatctctgcc tgctatattg taattgtatg taatattatt
     1261 tcctactaaa atatcagaat tatcatccat attccaattc catgttgtat ttgttggtgt
     1321 gattccggtt aatctaactt gactgttacc ttcttcaagg taagtagggt atacttcaaa
     1381 gtgtatgctg tcacgtgctt gcggaactag cagtacgcta
//
LOCUS       DAOPID010000264         1408 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_135558_length_1408_cov_3.628234, whole genome shotgun
            sequence.
ACCESSION   DAOPID010000264 DAOPID010000000
VERSION     DAOPID010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135558_length_1408_cov_3.628234"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PK641_07130"
     CDS             <1..285
                     /locus_tag="PK641_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010799990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HPG56149.1"
                     /translation="GVLDVTISSKLILDKILGIKDLRSDKRVDFVGGIRGLGEIKKRV
                     DSGEMQVALALYPVSMNQLIAIADSGNIMPPKTTWFEPKLRSGLIIHKLV"
     gene            301..1209
                     /gene="pyrB"
                     /locus_tag="PK641_07135"
     CDS             301..1209
                     /gene="pyrB"
                     /locus_tag="PK641_07135"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HPG56150.1"
                     /translation="MKSNGLVSIDDYTKEDILRILDLAAQFEKNPNTKLLEGKVIATL
                     FFEPSTRTRLSFETAINRLGGRVIGFSDAATSSSSKGETLKDTIKMVSNYVDLIVMRH
                     PVEGAARYASEVASVPIINAGDGANQHPTQTMLDLYSIKKTQGRLDNLIISMVGDLKY
                     GRTVHSLLMAMRYFNPTYHFVSPPELRIPEQYKLFCDKNKISYDELEDINSILPVSDI
                     LYMTRVQQERFTDLIEYEKVRNTYTLRNSMLDVTKDNLRILHPLPRITEIADDVDENP
                     KAYYFEQARNGVFARQAVICSTLGLV"
     gene            1228..>1408
                     /locus_tag="PK641_07140"
     CDS             1228..>1408
                     /locus_tag="PK641_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HPG56151.1"
                     /translation="MKASHTELIVRALENGTVIDHIPADKLFKVVWLLHLDNLPNQIT
                     IGNNLESKKIICKGII"
BASE COUNT          496 a          250 c          258 g          404 t
ORIGIN      
        1 ggagtacttg atgtaacaat atcatctaag cttattttag acaaaattct aggaattaaa
       61 gatttgcgaa gtgacaaacg tgttgacttt gttggcggca ttcgcggtct aggagagatt
      121 aaaaagcgtg ttgatagcgg agaaatgcaa gttgcacttg ccctataccc tgtttctatg
      181 aatcagttaa ttgcaatagc agattcaggt aacataatgc caccaaaaac cacatggttt
      241 gaacccaaac ttcgctcagg acttatcatt cacaaattgg tttaataata tacttacagc
      301 atgaaaagca atggtttagt atccatagac gactatacaa aagaagacat tctgcgtatc
      361 ctcgatttag cagctcaatt tgaaaaaaat ccaaatacca agttgctcga aggcaaagta
      421 attgcaacat tattttttga gccttcaaca cgtaccagac taagttttga aactgcaatt
      481 aatagactcg gtggacgtgt tatcggtttt tcagatgctg caacatctag ttcgtcaaaa
      541 ggcgaaacct tgaaagacac cataaaaatg gttagcaact atgttgatct aattgtaatg
      601 agacatccgg ttgaaggagc tgcaagatac gcatccgaag tagcaagtgt acctattata
      661 aatgccggag atggtgcaaa ccaacatcct acccaaacga tgcttgattt atactcgatt
      721 aaaaagaccc aaggtagact tgataatctt atcatcagta tggttggaga tttaaaatac
      781 ggacgcaccg tacattcgct attaatggct atgagatatt ttaatcctac atatcatttt
      841 gtatcaccac ctgaattacg tattccggaa caatacaagc tattttgcga taaaaataaa
      901 ataagctatg atgaacttga agatatcaat agtatattac ctgtatcgga tatattgtat
      961 atgacacgcg ttcagcaaga gcgctttacc gatttgattg aatacgagaa ggttcgcaac
     1021 acatatacct tacgtaatag tatgttggac gtaacaaaag acaatcttcg aatattacat
     1081 cccctacctc gcataactga gattgctgat gatgttgatg aaaatccaaa agcatattac
     1141 tttgagcaag ctagaaatgg cgtttttgca cgtcaggctg taatatgttc aactttggga
     1201 ttagtttaac ctttaattga gaaaaacatg aaagcaagtc atacagaact catagtaaga
     1261 gctctagaaa atggaacagt aattgatcac atccctgcag acaaattatt taaagttgta
     1321 tggttattac atttggataa tttaccaaat caaataacta ttggcaacaa ccttgaaagc
     1381 aaaaaaatca tctgcaaagg gataatta
//
LOCUS       DAOPID010000265         1405 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_136065_length_1405_cov_2.913333, whole genome shotgun
            sequence.
ACCESSION   DAOPID010000265 DAOPID010000000
VERSION     DAOPID010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136065_length_1405_cov_2.913333"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /gene="atpG"
                     /locus_tag="PK641_07145"
     CDS             <1..753
                     /gene="atpG"
                     /locus_tag="PK641_07145"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HPG56152.1"
                     /translation="QFLPYKDKLTEVLAQYIGSIEKEELNIPLAQSREIKKVALVAIS
                     SNTGLCGTFNTNTARLLNEALSEYKNKGIDIVVYPIGKKIADYAKRLNVEICTDFLHA
                     ADKPNYELSSDIAIKLADLFLSGKIDRVELLYNHYKNAGVQIPSREIFLPLSTQTDKN
                     TNTNTLYFVEPDRNTFINDLVPIVVRMRLYATILDSSTAEHGARTTAMQIASENAEKM
                     IGTIKQLYNRARQEVITTELIDIVGGSEALRK"
     gene            772..>1405
                     /locus_tag="PK641_07150"
     CDS             772..>1405
                     /locus_tag="PK641_07150"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPG56153.1"
                     /translation="MEVTRLFDLLSHYKEIKPNQDVALAYKRNGEWKKYSLDDYINQA
                     NYVSAGLLKLGVKKGDKIAIISSNRPEYNILDMGIMQIGAIPVPIYPTISASDYNYIL
                     NHAEIKYVFSEGEDLLRKIESILPTVPTLKGIYTFIDRGRHQYFAQLIDLGKSNLNLE
                     QIEMIKGTILPEDVACMIYTSGTTGMPKGVMLSHNNIVMQVMGVKDTPAKW"
BASE COUNT          533 a          230 c          238 g          404 t
ORIGIN      
        1 caatttctgc cgtataaaga caaacttact gaagtattag ctcaatatat tggttctatc
       61 gaaaaagaag aattaaatat accattagca caatcaagag aaatcaagaa agttgcacta
      121 gtagctattt catctaacac aggactatgt ggaacgttca acacaaatac agctcgtttg
      181 ttgaatgagg ctttatctga gtataaaaac aaaggaatag atattgtggt ataccctatc
      241 ggcaaaaaaa tagctgatta tgcaaaacgc ttgaatgtgg aaatctgtac tgatttttta
      301 catgcagcag acaagcccaa ttacgaattg tcatctgaca tagctattaa attagccgat
      361 ttgttcttat ccggaaaaat tgatcgtgtt gagttacttt ataatcatta taagaacgca
      421 ggagttcaaa tacctagtag agaaattttc ttacctttgt caacacaaac tgacaaaaat
      481 acaaatacca atactttgta ctttgttgaa cctgacagaa acacatttat taatgactta
      541 gtaccaattg ttgttcgtat gcgcttatac gcaaccatac tcgattcttc aacagccgag
      601 catggagcaa gaacaacagc aatgcaaatt gcatcagaaa atgcggaaaa aatgattggc
      661 actattaaac aattatacaa cagagcaaga caagaagtta tcacaactga acttattgat
      721 attgtaggcg gttctgaagc tttgcgtaaa taatcctaaa attttataaa catggaagta
      781 accagactat ttgatcttct gtcacattac aaagaaatta aaccaaatca agatgttgca
      841 ttagcataca aaagaaatgg cgaatggaaa aaatatagtt tggacgatta cattaatcaa
      901 gccaactatg taagtgcagg cttattaaaa cttggtgtaa aaaagggtga taaaatagca
      961 attatcagtt ctaatcgtcc tgaatacaat atattggata tgggaatcat gcaaataggt
     1021 gcaatacctg tgcctatata tccaactatc agtgcatctg actataatta tattttaaat
     1081 catgccgaaa taaaatacgt attttcggaa ggcgaagatt tattacgtaa aatagaaagt
     1141 attttaccaa cagttccaac actaaaaggt atttacacat ttattgaccg cggcagacat
     1201 caatattttg ctcaattaat tgatttaggg aaaagtaacc ttaatcttga acaaattgaa
     1261 atgattaaag gcacaatact acctgaggat gtcgcttgta tgatatatac atcaggtaca
     1321 acaggtatgc caaaaggtgt tatgctttca cataacaaca tagtaatgca agtaatggga
     1381 gtgaaagaca ctcctgctaa atgga
//
LOCUS       DAOPID010000266         1372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_141052_length_1372_cov_3.643888, whole genome shotgun
            sequence.
ACCESSION   DAOPID010000266 DAOPID010000000
VERSION     DAOPID010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141052_length_1372_cov_3.643888"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1372)
                     /locus_tag="PK641_07155"
     CDS             complement(<1..>1372)
                     /locus_tag="PK641_07155"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HPG56154.1"
                     /translation="SPVLVQDKRTNVYYIDFSFDTLKLGGSVLYQTLNKVGEKVPTVQ
                     NSEYFALAFEAIQKLINENLILAGHDISAGGIITTLLEMTFANKNGGLHVNFSDIAEK
                     DIVKILFSENPGVCIQVKDNLKVEKILKNAGIGYAKIAYPIDSRTLEIVKEEEHYNFD
                     IDALRDKWYKTSYLLDRKQSGEKCAKERFDNYKNQPLDLYIKNSFSGQMSQFGIKGTH
                     STSGIKAAIIREKGTNGDREMAFSLYLAGFDVKDVHMTDLASGRENLEDVNMIVFCGG
                     FSNSDVLGSAKGWAGGFRYNEKAKRALENFYKRKDTLSLGICNGCQLMIELNLINPEH
                     SKPTKMLHNNSHKFESEFIGINIPENNSVMFNTLKGSRLGIWVAHGEGKFSLPLAENE
                     YKVVAKYRYDGYPANPNGSDYNVAGICSADGRHLAMMPHLERAIFPWQWAYYPADRKN
                     DEITPWV"
BASE COUNT          402 a          262 c          224 g          484 t
ORIGIN      
        1 caacccatgg agttatttcg tcatttttac gatcagcagg atagtatgcc cattgccatg
       61 ggaatattgc gcgttctaag tgtggcatca ttgctaaatg gcgaccatct gcagaacaga
      121 ttccggctac gttataatcc gaaccgtttg gatttgcagg ataaccatcg taacgatatt
      181 tcgctacaac cttgtattcg ttttcagcta atggtaaact gaatttaccc tcgccatgtg
      241 caacccatat acctaatctg ctgcctttta aagtgttaaa catcacagag ttgttctcag
      301 gtatgtttat gccaataaat tctgactcaa atttatgtga gttgttatga agcatcttgg
      361 taggtttcga atgttcagga ttgataagat ttaattcaat cattaattga catccgttac
      421 agatacctaa gcttaatgta tctttgcgtt tatagaaatt ttcaagagca cgcttagctt
      481 tttcgttgta tctgaatccg ccggcccaac ccttggctga acctaagaca tcagaatttg
      541 aaaaacctcc acagaataca atcatattta catcttcaag attttcgcgt ccacttgcta
      601 aatcggtcat gtgtacgtct tttacatcga agcctgccaa atatagagag aatgccattt
      661 ctctatctcc atttgtaccc ttttcacgaa taatagctgc ttttattcca ctggtagagt
      721 gtgtgccttt aatgccgaat tgtgacattt gacctgagaa actatttttt atatacaaat
      781 caagaggctg atttttataa ttatcaaaac gttcttttgc acatttttct ccactttgtt
      841 tgcggtctaa caaatatgat gttttgtacc atttatcgcg taatgcatca atatcaaaat
      901 tgtagtgttc ttcttctttt acaatttcta atgtccgact gtctataggg taagctattt
      961 ttgcatagcc gatacctgca ttttttaaaa tcttttctac tttaagatta tctttaactt
     1021 gaatacaaac gccggggttt tcagagaata gtattttaac gatgtctttc tctgctatat
     1081 cagagaaatt tacgtgtaag ccaccatttt tattagcaaa tgtcatttct aataatgtgg
     1141 tgatgatacc acctgcagaa atatcgtgtc ctgctaatat caaattttca ttaattaatt
     1201 tttgaattgc ttcaaaagca agtgcaaagt attctgaatt ttgaacagta ggaacttttt
     1261 caccaacctt atttaaagtt tgatataaaa cagaaccacc tagtttaagt gtatcaaacg
     1321 agaagtcaat gtaataaaca ttagtgcgtt tatcttgtac caaaacagga ct
//
LOCUS       DAOPID010000267         1313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_151120_length_1313_cov_2.624006, whole genome shotgun
            sequence.
ACCESSION   DAOPID010000267 DAOPID010000000
VERSION     DAOPID010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151120_length_1313_cov_2.624006"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="PK641_07160"
     CDS             complement(<1..602)
                     /locus_tag="PK641_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HPG56155.1"
                     /translation="MNDKFKIILNGLFKENPIFVLLLGMCPTLGTTTSSLNGMSMGLA
                     TTFVLIGSNIVISLLKNLIPDKVHIPAYIIVIAAFVTVLQMCMEAYTPELYSSLGLFI
                     PLIVVNCIVLGRAEAYAAKHSVFDSALDGLGMGFGFTFALGLLGFVRELLGSGSILSI
                     PIINEQYNMLLFVLAPGGFIVLGLLIALSNHIKNNTRKNAL"
     gene            complement(631..1230)
                     /locus_tag="PK641_07165"
     CDS             complement(631..1230)
                     /locus_tag="PK641_07165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HPG56156.1"
                     /translation="MKKVKSNFTGMTISLTVISLIVGGLLAFVNDITTDPIKESKQQN
                     QSKAIKAVVPEFTYMETDTVEQMIIFKCYNENKLVGTAVSIVGQGFGGDFNIMVGFNA
                     NGEIINYTILSHKETPGLGSQMAEWFKKKSSIIGKSPKKGDLKVSKDGGDIDAITAAT
                     ITSRAFLNTINEAYKTYNSGNHDAVSSATSVEHHEEKNH"
BASE COUNT          412 a          229 c          207 g          465 t
ORIGIN      
        1 agtgcgtttt tacgcgtgtt gttttttata tgatttgaaa gggcaataag tagacctaat
       61 acaatgaaac cgccgggtgc caacacaaat aatagcatgt tatactgctc attaatgatg
      121 ggtattgata atatactgcc acttccaagt aattctctta caaatcctaa taagccaagt
      181 gcaaaagtaa atccgaatcc cattccaagt ccgtctaatg cagaatcaaa tacgctgtgt
      241 tttgcagcat atgcttcagc acgtccaagc actatgcaat tgactactat aagtggaata
      301 aatagtccta aactactata taattcagga gtatacgctt ccatgcacat ttgtagaacg
      361 gttacaaatg ctgctattac aataatatat gcaggaatgt gaaccttgtc aggaataaga
      421 ttttttaata atgaaatcac tatatttgag ccaataagta caaatgttgt agcaagtccc
      481 atactcatac cgttcaatga ggaagttgta gtacccaagg ttggacacat tccgagtagc
      541 agtacaaata tcggattttc tttaaataat ccgtttagaa tgattttaaa tttatcgttc
      601 atgatgtatg gttaatgagt atgtatttta ttaatgattt ttttcttcgt gatgttcaac
      661 tgatgttgca gaacttactg catcatggtt gccactatta tatgttttat atgcttcatt
      721 tatagtattc aaaaaagctc ttgaagttat cgttgcagca gtaatagcat caatatcgcc
      781 accatcttta gatactttta aatcgccttt tttaggtgat ttacctataa tagagctctt
      841 tttcttaaac cattcagcca tttgtgatcc aagtccgggt gtttccttat gagataaaat
      901 agtataattt attatttctc cattggcatt aaaaccgacc attatattaa agtctcctcc
      961 aaatccttga ccaacaatac ttactgctgt tccaacaagt ttgttctcat tatagcattt
     1021 gaatatgatc atttgttcaa cagtatccgt ctccatatat gtaaactcag gaactactgc
     1081 tttgatagcc ttgctttgat tttgttgttt tgattcttta ataggatcgg ttgttatatc
     1141 atttacaaaa gcaagtaatc caccaactat taaactaatt acagttagag aaatagtcat
     1201 tcccgtaaag tttgatttta cttttttcat ttttgccttc ctccaaaacg tttaggtttt
     1261 atataattgt taagtaaagg tgttacagca ttcataataa gtatggcaaa gga
//
LOCUS       DAOPID010000268         1308 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_152030_length_1308_cov_2.523543, whole genome shotgun
            sequence.
ACCESSION   DAOPID010000268 DAOPID010000000
VERSION     DAOPID010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152030_length_1308_cov_2.523543"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="PK641_07170"
     CDS             <1..1156
                     /locus_tag="PK641_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HPG56157.1"
                     /translation="RVDGMDIILPSGDKLNIKGTNLGHWLNPEGYMIGIHKNANSASR
                     INQAFCEMVGPEKTAEFWQKFKDNYITQSDIAFIKSSGANTIRIPFHYKLFTREDYMG
                     LNDENEGFKRLDSLVTWCKKEQIYLILDMHDAPGGQTGDNIDDSYGYPWLFESPRCQD
                     LFVDIWVRISEHYKNETIILGYDLMNEPIATYFKDNEEELNKELEPLYIRTTKAIREV
                     DKNHIIILGGAQWNSVFSVFTDWKFDDNIMYSCHRYKGGTNKKAIQSFMDFRDSTNLP
                     MWMGETGHNTYEWLRDFRITMEENNIGWTYWPYKKMGESSFVNVKEPANWNLIIQYAE
                     SDRSWYALIRKNRPDAQLINKAMDEYIENIKFKNCIVESKYIEALGLEAE"
BASE COUNT          482 a          208 c          249 g          369 t
ORIGIN      
        1 ccgtgtagat ggaatggata ttatattgcc gtcgggtgat aagctcaata ttaaaggtac
       61 aaatctagga cattggctta atcccgaagg atatatgata ggtattcata aaaatgcaaa
      121 ttctgcatca cgtattaatc aagcattttg cgaaatggtc ggcccggaaa agaccgctga
      181 gttttggcaa aaattcaaag ataattatat cactcaaagc gatatagcat ttattaaatc
      241 aagcggtgct aacacaatac gcattccttt tcattataaa ttatttactc gcgaagatta
      301 tatgggctta aatgatgaga acgaaggatt caagcgactt gacagccttg taacctggtg
      361 taaaaaagaa caaatctatc ttattttaga tatgcatgat gcacccggag gtcaaacggg
      421 cgataatatt gatgatagtt acggctatcc ttggttattt gaatcgccaa gatgtcaaga
      481 tttatttgta gatatctggg taagaatttc tgaacattat aaaaatgaga ccattatttt
      541 aggatatgat ttaatgaacg aacctattgc cacctacttt aaagataatg aagaagaact
      601 taataaagag cttgaacctc tttatataag aactacaaaa gcaatacgtg aggtcgataa
      661 aaatcatatc attatacttg gaggcgcaca atggaacagt gttttcagtg tatttacaga
      721 ttggaaattt gacgataaca taatgtattc atgccacaga tacaaaggag gtacaaacaa
      781 aaaggccatt caaagtttta tggattttag agatagtaca aacctgccta tgtggatggg
      841 agaaacagga cataatacat acgaatggtt acgcgatttt cgcataacta tggaagagaa
      901 caatatcggt tggacttatt ggccttataa aaaaatggga gaatcatcct ttgtaaacgt
      961 aaaagaacct gcaaattgga atctgattat tcaatacgca gaaagcgatc gtagttggta
     1021 tgctctaata cgtaaaaata gacctgatgc acaattaatc aacaaagcaa tggatgaata
     1081 cattgaaaat ataaagttca aaaactgcat agttgaatca aaatacatcg aagcattagg
     1141 actagaggcc gaataaacta acaagcttac aaaataaaaa agagactgcc atatcatagt
     1201 atatgtgcag cctctgaaat tatattaaga tattcgctta agcgtttcta ccgcagcaat
     1261 ttttgtattt tttgccactg ccgcaaggac acggatcatt acgaccta
//
LOCUS       DAOPID010000269         1301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_153196_length_1301_cov_4.308989, whole genome shotgun
            sequence.
ACCESSION   DAOPID010000269 DAOPID010000000
VERSION     DAOPID010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153196_length_1301_cov_4.308989"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..>1301
                     /locus_tag="PK641_07175"
     CDS             178..>1301
                     /locus_tag="PK641_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG56158.1"
                     /translation="MNKKYSLLFCIYILSTYSLFASNVSYFQAPTKSVQFEAANVYLM
                     IDNERTSKEGWTGRDSLRLMLEENEINKVVDKVGRFAVWKFVPAYIYCSDASKKRAMV
                     EILVSNIPLESSADSIKGNGDLIAVPYKIKCRYRISDNEGHVLNEIDLGVLQGVDSTK
                     NEQYAKVLPASSLVGIHAAYRRVRQDVFSKYGFGTFETPFNLADIPGISNLSELKSRV
                     IDAVENKKHLLLTKNEKNILRLYTDVLSYNIGLCKEKQLVDAYHNLSICYAWLEEPEK
                     AREAYEQYGQYVEGELALSEYNALRPFVLNYPVAAVKYASLFNVINRNMSRFVDYYTY
                     NDLICQVFRLNYPFQFFPLNDCKGKPKSIEGNITRLNNTP"
BASE COUNT          426 a          197 c          246 g          432 t
ORIGIN      
        1 aacataatca caccccgaat cccacgggcg aatgcaattc gcccctacac attacgcgtc
       61 cttcacccta taaaaaatgt tattctacga tgctaattca tggcttttta aacatttttg
      121 cccgatagaa atataaaatt attacctttg catcattact aataatgctc gtatcaaatg
      181 aataaaaaat atagccttct tttttgcata tacatactat ctacatacag tttgtttgca
      241 agtaatgtat cttattttca ggcacctaca aagagtgtac aatttgaagc tgctaatgtg
      301 tatttgatga ttgataacga acgtacttct aaagagggat ggacgggaag agattcttta
      361 cgcttgatgc ttgaagaaaa tgaaataaat aaggtggttg ataaggttgg tcggtttgct
      421 gtatggaaat ttgttcctgc ttatatttat tgtagtgatg ccagtaagaa acgagcaatg
      481 gttgaaatac ttgtatcaaa tatacctttg gaaagtagtg ctgatagtat aaaagggaat
      541 ggtgatttga tcgcggttcc gtataaaatt aagtgcagat accgtatcag cgataatgaa
      601 ggtcatgttt taaatgaaat tgatttaggc gttttacagg gtgttgattc aacaaaaaac
      661 gaacaatatg ctaaggtatt gccggcatca tcattggtag gaatacatgc agcatacaga
      721 agagttagac aagatgtgtt ttcgaaatat ggtttcggta ctttcgaaac tccgtttaat
      781 ttggcggata tacccggaat ttcaaactta tcagaactta aatctcgtgt tattgatgca
      841 gtagagaata aaaagcatct tcttttaaca aaaaacgaga aaaacatatt aagactatat
      901 actgatgtct tatcgtacaa cataggactt tgtaaagaga aacaattggt tgatgcatat
      961 cataatttat ctatttgtta tgcatggctt gaagagcctg aaaaagctcg agaagcatat
     1021 gaacaatatg gtcaatatgt tgaaggtgag ctggctctgt ctgaatataa cgctttgcgt
     1081 ccctttgtgt tgaattatcc tgttgcggct gttaaatatg catctttatt taacgtgatc
     1141 aatagaaata tgtctcgttt tgttgattat tatacatata atgatcttat ttgtcaggta
     1201 ttcagattga attatccttt ccaatttttc cctttaaatg attgtaaggg aaaaccaaag
     1261 tctattgagg gtaatataac tcgtttaaat aatacgccta t
//
LOCUS       DAOPID010000270         1290 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_155262_length_1290_cov_3.310931, whole genome shotgun
            sequence.
ACCESSION   DAOPID010000270 DAOPID010000000
VERSION     DAOPID010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155262_length_1290_cov_3.310931"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="PK641_07180"
     CDS             complement(<1..506)
                     /locus_tag="PK641_07180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPG56159.1"
                     /translation="MLSICIPIYNYDIRPLFYNLIKQKSTLTIPIEIVLADDGSDYSY
                     TSINEEIASLADVYLKMDKNVGRAKIRNILSDNAQYDYLLFIDCDSVIVSDTFLSDYV
                     AQIDGKEKVVYGGVCYSDNASWNEKLRYLYGLKRECRPVEFRNSHPYQTFLSGNFLIH
                     KKVFEKVR"
     gene            596..>1290
                     /locus_tag="PK641_07185"
     CDS             596..>1290
                     /locus_tag="PK641_07185"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPG56160.1"
                     /translation="MIKHLRNIVCVVFGLLFIAPNMQSEEANKSIGICLSGGGAIGFT
                     HLGVLQALEENNIKPQYVSGSSMGALIGTLYAAGYSPSEIMVLVEQKQLYKIINFVSS
                     PKSRKGLSSMKNVQKILLKYVPHNNFEMLNKKLYICVANLTTGQCEIISSGKQLSEYA
                     CASAAIPVVFEPQEINGSQYADGGLLNNLPAEPLRQICDVVIGVDVLEYPKNKKISDK
                     MESLYLSILVNNA"
BASE COUNT          465 a          215 c          217 g          393 t
ORIGIN      
        1 aaacgaactt tttcaaaaac ctttttatga atcagaaaat taccggataa aaatgtttgg
       61 tatggatgtg aatttctaaa ttctacaggt ctgcattccc ttttcaaacc atataagtag
      121 cgcaactttt cgttccatga tgcattgtca gaataacaaa ctcctccata aacaactttt
      181 tctttaccat caatttgggc aacataatca gacaagaaag tatctgagac tataaccgaa
      241 tcgcagtcaa taaataacaa ataatcgtat tgagcgttat ccgacaaaat attacgaatt
      301 tttgcacgcc ccacgttttt gtccatcttt aaataaacat cagcaagtga tgcaatttct
      361 tcatttatag atgtatacga ataatcagag ccatcatcag ctaagacgat ttcaataggt
      421 atagtaagag tagacttttg ttttattaaa ttataaaaga gcggtctaat gtcgtaatta
      481 tatattggga tgcatataga gagcatgtcg ttttccacaa aagtttcaca aaagtaagta
      541 aattattgct catacgaaca aaatatacga tatttgtcaa taaaataaga cattcatgat
      601 taaacatctg agaaacatag tttgtgttgt ttttggatta ttatttatcg ctcctaatat
      661 gcaatcagaa gaagctaaca aaagtattgg catctgccta agtggaggtg gagctattgg
      721 atttacacat cttggcgtac tacaagcctt ggaagaaaat aacattaaac ctcaatatgt
      781 atccggatca agcatgggag cgttaatagg aacattgtat gcagccggat attctccttc
      841 agaaataatg gttttagtag aacaaaagca attgtataaa ataattaatt tcgtatcatc
      901 tcctaaatca agaaaaggtt tgtcgagcat gaagaacgta caaaaaatct tgctcaaata
      961 tgtcccgcac aacaatttcg aaatgcttaa taaaaaactg tatatatgcg ttgctaattt
     1021 aactaccggt cagtgcgaaa tcatttcaag cggtaaacaa ctcagcgaat atgcatgtgc
     1081 atcagctgca attcctgttg tattcgaacc acaagaaata aatggcagcc aatatgcaga
     1141 tggaggttta ttaaacaatc taccggctga accattaagg caaatttgtg acgtagttat
     1201 tggtgttgac gtacttgagt atccgaagaa taaaaaaata tcagacaaaa tggaatcttt
     1261 gtatttgtct atattagtca ataatgcaga
//
LOCUS       DAOPID010000271         1276 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_157918_length_1276_cov_2.988534, whole genome shotgun
            sequence.
ACCESSION   DAOPID010000271 DAOPID010000000
VERSION     DAOPID010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157918_length_1276_cov_2.988534"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            336..944
                     /locus_tag="PK641_07190"
     CDS             336..944
                     /locus_tag="PK641_07190"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPG56161.1"
                     /translation="MNKDDIIQSQTEPLLNYFQKVIPLNPKEKQLVIQLFKPRLYRKK
                     QYVLQEGDVCNQFNFIVRGCLRMYKVDDKGTTYILQFATENWWLSNIGSFHERKPSEL
                     NIDALEDTMVLQISHENLLLLYTSAPKFDRIFRVLIENSFVSLQKRLLQNISSTASER
                     YESFLDSYSHLTNRLPQTQIASFLGITPEFLSRLRNQQIKKP"
     gene            1047..>1276
                     /locus_tag="PK641_07195"
     CDS             1047..>1276
                     /locus_tag="PK641_07195"
                     /inference="COORDINATES: protein motif:HMM:NF013641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HPG56162.1"
                     /translation="MTTSSKVAVIGLGNIGKTIAQNLVKGNRPVIIASRKLEDATALA
                     KELGSLATAAETGEAIQNADIIILSVWFNIIQ"
BASE COUNT          443 a          216 c          246 g          371 t
ORIGIN      
        1 ccggtatatt ggctgcacaa attttagcct tgcatgatcc ttccatcgct ggcaagttag
       61 aagcttacaa agaataccag gatgatattg taatggagag tgtgaagaat gtgcagatgc
      121 tgggttttac aaatgatttt gataagtaag gttgacggtt tggggtttgg aattggaggt
      181 tgttggagaa tttgggttct tagggcaaag ccgatgtaaa ccagggcaat tatttgtcaa
      241 gctctgttga caaatggact gctatttgat tctgcataaa tagaagttgt gatcaataca
      301 aaagaaagca acattctaat caacagaaaa atgacgtgaa caaagacgac attatacaat
      361 cacaaactga accgctgttg aattactttc aaaaagtaat tccattaaac cctaaggaaa
      421 aacaattggt tatccaatta ttcaaaccaa gactctaccg aaagaaacaa tatgttttgc
      481 aggaaggcga tgtgtgcaac cagttcaatt ttattgtgag gggttgcctg cgtatgtaca
      541 aagtggacga caaaggaact acatatattt tacaatttgc taccgaaaac tggtggttga
      601 gtaatattgg cagcttccat gaacgcaagc cttcagaact caatattgat gctttggaag
      661 acacaatggt tttgcagatt agtcacgaga atttactttt actttataca agcgcaccca
      721 agttcgaccg tatcttcagg gtgttaatcg aaaatagttt tgtatcactt caaaaacgat
      781 tactacaaaa tatcagttct actgcatcgg aaagatatga gtcatttctt gacagttatt
      841 cgcatctcac caaccggcta ccacaaacac aaattgcttc gtttttgggc attacacctg
      901 agtttttaag tcgtttaagg aatcagcaaa ttaaaaaacc ttaatctaca tcaatactat
      961 ttcttaatgc agtttattgg agagaacctt ctttccaata aaactttgca ttatcaaaat
     1021 atcattaaaa tttaaaaaga aatagtatga caacatcttc aaaagtagcc gtgatcggtt
     1081 taggaaatat cggaaaaaca atagctcaga atctcgttaa aggcaacaga ccagtaatta
     1141 ttgcaagccg taaattagag gatgcaacag cattggcaaa agaattagga agtcttgcaa
     1201 cagcagctga aactggtgaa gcaattcaaa atgcagacat aattatactt tctgtttggt
     1261 ttaatatcat tcaaga
//
LOCUS       DAOPID010000272         1182 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_178167_length_1182_cov_3.063886, whole genome shotgun
            sequence.
ACCESSION   DAOPID010000272 DAOPID010000000
VERSION     DAOPID010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178167_length_1182_cov_3.063886"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PK641_07200"
     CDS             complement(<1..301)
                     /locus_tag="PK641_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG56163.1"
                     /translation="MFDLDNWQEIWTTITRNKLRSILTAFGVFWGIYMLVVMAGMGNG
                     IQNKMIGELGKFSVNSTFVFSRNTTEPYAGFAKGREIVFNDADFAAVIKQFPELEA"
     gene            complement(304..>1182)
                     /locus_tag="PK641_07205"
     CDS             complement(304..>1182)
                     /locus_tag="PK641_07205"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPG56164.1"
                     /translation="PGYDKMRNMYVISGRLLDESDERECRKVIILHPKTAETLFKGKD
                     PIGKYVNVQGLLFKVVGIYDIKGGSGWNKSYYIPFSTSQKIFNPGGYITDLTFAINGI
                     ENAAQSEELSNKLRRMLSARMQFDPEDKNAMYIEDRMSSFEQSQVVFNGISLFIWIIG
                     IGTLIAGIVGISNIMLITVKERTREFGIRKALGATPSSIIKLVVLESLTITLLFGYIG
                     MLLGVALTETVAKVMSSMPVESGDHNMNMFMNPTVNLDVAVAAMAILVLAGVIAGYVP
                     AHKAVKIKPIEAMQAK"
BASE COUNT          363 a          250 c          169 g          400 t
ORIGIN      
        1 ctgcttctaa ttcaggaaac tgtttaatta ctgcagcgaa atctgcatca ttaaatacta
       61 tttcacgtcc tttggcaaat ccggcataag gttcggttgt attcctcgaa aaaacgaagg
      121 ttgaattaac tgaaaatttt cctaattcac caatcatttt attttgaata ccattgccca
      181 ttccggccat aactaccaac atatatatgc cccaaaacac accgaaggcg gtaaggatac
      241 tacgcaattt attgcgagtt atagtagtcc atatttcttg ccaattatct aaatcaaaca
      301 taattatttt gcttgcattg cttctatagg tttaatctta acagccttgt gtgcaggtac
      361 atacccggct attacaccgg ccaaaaccaa tatcgccatc gctgctacag caacatccaa
      421 atttacggtt ggattcataa acatattcat attatgatca ccactttcaa ccggcattga
      481 tgacataact ttagctacag tttcggttaa tgcaactcct aataacatgc ctatatatcc
      541 aaataatagt gttattgtaa gagattctaa tacgactagt tttattattg aacttggagt
      601 tgctcctaag gctttacgaa taccaaattc tctagttctt tcttttacag ttatcaacat
      661 aatattgcta atacctacaa tacctgcgat taatgtgcct attcctatta tccatataaa
      721 taatgagatt ccgttaaaaa ccacttgaga ttgttcgaaa gaagacatcc tatcctcaat
      781 atacatcgca tttttatctt ccggatcaaa ttgcatgcgt gctgacaaca ttcgtctaag
      841 tttattcgac aattcctcac tttgagctgc attttcaatt ccgttaattg caaatgtcag
      901 gtctgtaatg tatcctcccg gattaaatat tttttgagaa gtagagaatg gtatgtagta
      961 agatttgttc catccggagc ctccttttat gtcatatata ccaacaacct taaacaaaag
     1021 accttgcaca ttaacatatt tgcctatcgg gtctttacct ttaaacaaag tctcggctgt
     1081 ttttggatgt aatattatta cctttctaca ttcacgttcg tctgattcat caagtaaacg
     1141 tcctgatata acatacatat tacgcatttt atcataaccg gg
//
LOCUS       DAOPID010000273         1142 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_188154_length_1142_cov_2.767249, whole genome shotgun
            sequence.
ACCESSION   DAOPID010000273 DAOPID010000000
VERSION     DAOPID010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188154_length_1142_cov_2.767249"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..320)
                     /gene="atpC"
                     /locus_tag="PK641_07210"
     CDS             complement(75..320)
                     /gene="atpC"
                     /locus_tag="PK641_07210"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HPG56165.1"
                     /translation="MTLEILTPEKKLYSGEASAVTLPGIDGKFQLLERHAPIISALGK
                     GKVVYKSATGVNEIEISGGFAECQNNKVIVLVEGALA"
     gene            complement(348..>1142)
                     /gene="atpD"
                     /locus_tag="PK641_07215"
     CDS             complement(348..>1142)
                     /gene="atpD"
                     /locus_tag="PK641_07215"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HPG56166.1"
                     /translation="QMNEPPGSRARVALSGLTIAEYFRDGDKNDAKGGRDILFFIDNI
                     FRFTQAGSEVSALLGRMPSAVGYQPTLATEMGLMQERITSTKRGSITSVQAVYVPADD
                     LTDPAPATTFAHLDATTVLNRKIAEQGIYPAVDPLDSTSRILNEAVLGKEHYGCAMRV
                     KEILQRYKELQDIIAILGMDELSEEDKLVVHRARRVQRFLSQPFHVAEQFTGLAGVLV
                     PIEDTIKGFNMILNGDVDKYPEAAFNLVGSIEDAIAKGEKMLAEVK"
BASE COUNT          318 a          245 c          215 g          364 t
ORIGIN      
        1 aattttggtt gtagagcgaa acaaaaaagc catctgaaat cagatggctt ttttgttatt
       61 gagtatgttt aatattaggc taatgctcct tctactaata caattacttt attgttctga
      121 cattctgcaa aaccaccgga aatttcaatt tcatttacac ctgttgcaga tttatatact
      181 actttacctt taccaagggc agagataata ggagcgtgtc tttccaacaa ttgaaatttc
      241 ccgtcaatac ctggaagtgt tacagcagat gcctctcctg aatatagttt tttctccggt
      301 gttaagattt ctaaggtcat tattaatgtc gttttttgtt acgatgatta ttttacttca
      361 gccaacattt tttctccttt agcgattgca tcttcgatag aacctaccaa gttgaaagca
      421 gcttcaggat atttatctac gtctccgttc aaaatcatat taaacccttt gatggtatct
      481 tcgataggta ccaatacccc tgccaaacct gtaaattgct cagcaacgtg gaaaggttga
      541 gataagaaac gttgaacacg tcttgcacgg tgaactacca atttatcttc ttcacttaac
      601 tcgtccatac ccaagatggc gatgatatct tgcaattctt tataacgttg taagatttct
      661 ttcacgcgca ttgcgcaacc ataatgctct tttcctagaa cggcttcgtt aaggatacga
      721 gatgttgaat ccaatggatc cacagcaggg tagatacctt gctcagcaat tttacggttc
      781 aataccgttg ttgcatctaa gtgggcaaat gttgtagccg gagccgggtc agttaaatca
      841 tccgcaggaa cgtataccgc ttgtacagaa gtaattgaac ctctttttgt agaagtgata
      901 cgctcttgca tcaatcccat ctctgtagcc aacgttggtt ggtaacctac cgcagaaggc
      961 atacgaccta atagagccga tacctcagaa cctgcttggg tgaaacggaa gatattgtcg
     1021 ataaagaaca agatatctcg tccgcctttt gcatcatttt tatctccgtc acggaagtat
     1081 tctgcaattg tcaagcctga caaagctact cgagcacgag atcccggtgg ttcgttcatt
     1141 tg
//
LOCUS       DAOPID010000274         1116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_195225_length_1116_cov_2.359095, whole genome shotgun
            sequence.
ACCESSION   DAOPID010000274 DAOPID010000000
VERSION     DAOPID010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195225_length_1116_cov_2.359095"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..1007)
                     /locus_tag="PK641_07220"
     CDS             complement(195..1007)
                     /locus_tag="PK641_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG56167.1"
                     /translation="MRKVILFFAAISCQTIFSQELFVVTDPASNVPANSLAVNVMQSA
                     FKEKFEPGYNYHLMPEVTYGINKNLMVRGSAFISTRTNALYAEGGGFMAKYRFYSTDD
                     LNSHFRLATYGRFSFNRADIHQEQIEIVGHNTGFETGIIATKLVKKVAISSSLSFEKA
                     LDNKPKYPFPDTIGDNATNYTLSFGKLMYPKKYTSFKQTNINLMVEFVGQTINENGKS
                     YLDVVPAVQFIFNSQARLDLAYRQELISSMIRSAPNGFYFNLYYTFFNLKKQ"
BASE COUNT          347 a          186 c          174 g          409 t
ORIGIN      
        1 catttggaac attcttctcc tttttttcct gtaatctctg ggtgcatgga acacgcatat
       61 atttcatttg aatttgttga agtttctgtg gtcttgtttt ctgtctcttt gtttttttga
      121 ttacaagaaa ctaaagtgat agctataata gctgatagaa taagtgcttt catgacatgt
      181 tattattaag atttttattg tttttttaaa ttgaaaaaag tataatacaa attgaagtaa
      241 aacccatttg gagcagaacg tatcattgaa cttattaact cttgacggta ggcaagatct
      301 aatcgtgcct gactgttaaa aatgaattgt accgccggca caacgtctaa ataggattta
      361 ccattttcgt tgatggtttg acctacaaat tcaaccatta aattgatatt ggtttgcttg
      421 aaactcgtat attttttagg gtacatcaat tttccaaaag acaaagtata gttcgttgca
      481 ttatcaccaa tagtatccgg aaaaggatat tttggtttgt tatccaatgc tttttcaaaa
      541 ctaagcgatg aacttatggc tacctttttg accaactttg ttgcgattat cccggtttca
      601 aaaccagtat tgtgtcctac tatttcaatt tgctcttgat gaatatcagc acgattgaag
      661 ctaaatcttc cataagttgc taagcgaaag tgactgttta aatcatctgt agaataaaac
      721 ctgtacttag ccataaaacc accaccttct gcatacaatg cattagttct tgtgctgata
      781 aaagcggaac cacgaaccat taaattttta tttataccat aagttacctc gggcattaaa
      841 tgatagttgt aacctggctc aaatttttct ttaaaagcag attgcatcac atttacggct
      901 aatgaattag caggaacatt acttgcagga tctgttacta caaataattc ttgactaaaa
      961 atagtttggc aactgatggc tgcaaaaaat aaaattacct ttctcattag tttatcattt
     1021 taatgtgaaa atcatcttca ttattcgaag catacgtatc aatattctta taggactttg
     1081 atagtttttt aaattctttc tcggttacat atcatt
//
LOCUS       DAOPID010000275         1079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Enterocola sp. isolate AMR_MDS_3312
            NODE_206105_length_1079_cov_4.077148, whole genome shotgun
            sequence.
ACCESSION   DAOPID010000275 DAOPID010000000
VERSION     DAOPID010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865047
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Enterocola sp. (wastewater metagenome)
  ORGANISM  Candidatus Enterocola sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Candidatus
            Enterocola.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,446
            CDSs (total)                      :: 1,420
            Genes (coding)                    :: 1,399
            CDSs (with protein)               :: 1,399
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 4 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Candidatus Enterocola sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_206105_length_1079_cov_4.077148"
                     /isolate="AMR_MDS_3312"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:3022872"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..602)
                     /gene="rimM"
                     /locus_tag="PK641_07225"
     CDS             complement(96..602)
                     /gene="rimM"
                     /locus_tag="PK641_07225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HPG56168.1"
                     /translation="MIKKEDLFVAGKLLKTHGVKGEICLLINVDVLADSNYFICEIDG
                     LLVPFYIEKRRAKSDTVDIVKIEGVDSESDASIMLGSLVYLPKELQEEIEQASYSFFI
                     GYSVVADGQNLGKIVSVDDKTANVLFEIKSATDDYMIPVTEDFIESIDKKKKCIYMCL
                     PDGLLSIN"
     gene            complement(599..>1079)
                     /locus_tag="PK641_07230"
     CDS             complement(599..>1079)
                     /locus_tag="PK641_07230"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG56169.1"
                     /translation="KSYAYGSAFWQYTEDIDKIKSNNSVLYVDRKLFVEKNGILTPID
                     SSNVKVGDRICVRITLKTDRDIEFVHLKDLRAAGTEPQKQMSEYVWDRGLSYYKVVKD
                     ASVEMFFTNLRKGAYILEYDLWASLPGKYQAGYASVQCLYSPDLRANSSSLNFTILK"
BASE COUNT          339 a          211 c          129 g          400 t
ORIGIN      
        1 cgctgactta atataatcat gtttttttta tttttctaga ctacaatttt gagttgcaaa
       61 tttacttctt ttttctgaaa gaaatagtta aatagctaat taattgacaa cagcccgtcg
      121 ggaaggcaca tataaataca tttcttcttt ttatcaatcg attctataaa atcctctgtt
      181 acaggaatca tataatcatc tgtagcactt ttaatttcaa ataaaacatt tgctgtttta
      241 tcatcaaccg atactatttt acctaaattt tgaccatcgg ctacaaccga atatcctata
      301 aagaatgaat acgatgcttg ttctatttct tcttgtaatt ctttaggaag atacactaaa
      361 ctacctaaca ttatcgaggc atcagactca gaatcaacgc cttctatttt tacaatatca
      421 accgtatcac tttttgctct tcgtttttct atataaaagg gtaccaacaa tccgtcaatt
      481 tcgcaaataa aataattact atctgccaaa acatcgacgt taattaataa acatatttct
      541 cctttaactc cgtgagtttt tagaagtttg cctgcaacaa ataaatcttc tttttttatc
      601 attttaatat tgtaaaattc agacttgatg aattagctct taaatcagga gaatataagc
      661 actgtacaga agcatagccg gcttgatatt tacccggtaa tgatgcccat aaatcgtatt
      721 caagtatgta tgcacctttt cgtaaattag taaaaaacat ttccacagat gcatccttta
      781 ccactttata gtacgataat cccctatccc atacatactc agacatttgt ttttgaggct
      841 cggttcctgc tgctctcaaa tcttttagat gtacaaattc tatgtcgcga tctgttttta
      901 atgtaattct tacacatatc ctatcgccta ctttcacatt cgaactatct attggcgtca
      961 atattccatt tttttcaaca aacaattttc tatcaacata taaaactgaa ttattcgatt
     1021 tgattttgtc aatatcttct gtatattgcc agaaggcact tccgtatgcg tatgattta
//
